Interleukin-2 gene-encoded stromal cells inhibit the growth of metastatic cholangiocarcinomas.
OBJECTIVE
To demonstrate bone marrow stromal cells (BMSCs) can be used as an attractive target for genetic modification in the treatment of malignant diseases.


METHODS
Using a hamster model of biliary cancer, we investigated the therapeutic effects of interleukin-2 (IL-2) gene-modified BMSCs. Syrian golden hamsters were injected via the femoral vein with 5 x 10(5) cells of the KIGB-5 biliary cancer cell line (n=20). One week later, the hamsters were injected intraperitoneally with BMSCs containing Ad/hIL-2 and Ad/ Delta E1, unmodified BMSCs, or RPMI only (control) and observed for 12 wk (n=5 /each group).


RESULTS
All hamsters treated with BMSCs containing Ad/hIL-2 survived with no evidence of the disease during this period. In contrast, hamsters in the other three groups showed disseminated metastases involving the lungs as early as 4 wk.


CONCLUSIONS
Ad/IL-2 therapy is effective in the treatment of biliary cancer.
Kim
Myung-Hwan
MH
Department of Gastroenterology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
Lee
Sang Soo
SS
Lee
Sung Koo
SK
Lee
Seung-Gyu
SG
Suh
Chul-Won
CW
Gong
Gyung-Yub
GY
Park
Jung-Sun
JS
Kim
Young-Hoon
YH
Kim
Sang-Hee
SH
D000818:Animals; D001650:Bile Duct Neoplasms / Q000473:pathology / Q000628:therapy*; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000556:secondary / Q000628:therapy*; D006224:Cricetinae; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D008175:Lung Neoplasms / Q000556:secondary; D008647:Mesocricetus; D017154:Stromal Cells / Q000502:physiology*; D014161:Transduction, Genetic; D023041:Xenograft Model Antitumor Assays
D007376:Interleukin-2