Mass spectrometry and protein analysis.
Mass spectrometry is a central analytical technique for protein research and for the study of biomolecules in general. Driven by the need to identify, characterize, and quantify proteins at ever increasing sensitivity and in ever more complex samples, a wide range of new mass spectrometry-based analytical platforms and experimental strategies have emerged. Here we review recent advances in mass spectrometry instrumentation in the context of current and emerging research strategies in protein science.
Domon
Bruno
B
Institute of Molecular Systems Biology, ETH Zurich, CH-8093 Zurich, Switzerland.
Aebersold
Ruedi
R
D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D010449:Peptide Mapping; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods
D011506:Proteins; D020543:Proteome