Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model.
OBJECTIVE
The diagnostic feasibility of optical coherence tomography (OCT) and laser-induced fluorescence (LIF) have been evaluated for human colorectal cancer. This study applies these technologies to a murine model of colorectal adenoma.


METHODS
The lower colon of 10 Apc(Min) and two C57BL/6J mice was surveyed over five 4-week intervals using a prototype 2.0 mm diameter OCT-LIF endoscope-based system. Four categories were histologically classified: control C57BL/6J, adenomatous, non-diseased regions of adenomatous, and non-diseased Apc(Min). OCT images were compared to histology. Spectra from the four categories were compared via the Student's t-test.


RESULTS
Three Apc(Min) and two control mice completed the study. One adenoma was histologically identified; OCT visualized mucosal thickening/abnormal mass development over the imaging timepoints. LIF spectral comparisons revealed decreased 405 nm intensity and the presence of a peak at 680 nm in the adenomatous Apc(Min).


CONCLUSIONS
These preliminary data indicate endoscopic OCT-LIF has the potential to identify colorectal adenomas in murine models.
Hariri
Lida P
LP
Division of Biomedical Engineering, The University of Arizona, Tucson, AZ 85721, USA.
Tumlinson
Alexandre R
AR
Besselsen
David G
DG
Utzinger
Urs
U
Gerner
Eugene W
EW
Barton
Jennifer K
JK
D000236:Adenoma / Q000473:pathology*; D000818:Animals; D015179:Colorectal Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D004724:Endoscopy; D007834:Lasers; D051379:Mice; D008810:Mice, Inbred C57BL; D010865:Pilot Projects; D013050:Spectrometry, Fluorescence; D041623:Tomography, Optical Coherence