RNA aptamers directed against oligosaccharides.
Nucleic acid molecules are designed to interact predominantly with proteins or complementary nucleic acids. Interaction of nucleic acids with carbohydrates, abundant constituents of glycoproteins and glycolipids, are not common in cells. Biomedical applications of nucleic acids targeted against oligosaccharides, which are involved in the function of receptors, immune answer, host interaction with invading infectious agents, and cancer metastasis, are feasible. In vitro selection of nucleic acids interacting with oligoand polysaccharides is a promising strategy to identify potential inhibitors of biochemical recognition processes in which carbohydrates are involved. Several RNA and DNA aptamers directed against carbohydrates have already been isolated and characterized. The results are summarized in this article, and an attempt is made to draw initial conclusions concerning the perspectives of the outlined approach.
Sprinzl
M
M
Laboratorium für Biochemie, Universität Bayreuth, 95440 Bayreuth, Germany. mathias.sprinzl@uni-bayreuth.de
Milovnikova
M
M
Voertler
C S
CS
D000818:Animals; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000037:antagonists & inhibitors; D006801:Humans; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000037:antagonists & inhibitors*; D012313:RNA / Q000494:pharmacology*
D002241:Carbohydrates; D009844:Oligosaccharides; D012313:RNA