title: STRO-1+ mesenchymal precursor cells located in synovial surface projections of patients with osteoarthritis.
abstract: OBJECTIVE
  To investigate the presence of mesenchymal precursor cells (MPCs) in synovial surface projections of patients with osteoarthritis (OA), to characterize their phenotype and to show their localization.
  
  
  METHODS
  Progenitor cells in synovial surface projections were identified by immunohistochemistry, morphometric analysis and confocal laser scanning microscopy using the following phenotypic markers: STRO-1, CD34, and alpha smooth muscle actin (alpha-SMA).
  
  
  RESULTS
  In the synovial tissue of all 21 patients with OA MPCs were detected. Immunohistochemistry and subsequent morphometric analysis showed that approximately twice as many STRO-1+ cells/mm2 were observed in synovial tissue of patients with OA as compared to healthy organ donors and that number of STRO-1+ cells/mm2 correlated with total cell number/mm2. Interestingly, in the synovial tissue of patients with OA, twice as many STRO-1+ cells/mm2 were found in synovial surface projections as compared to the sublining area without villi. Using confocal laser scanning microscopy two populations of STRO-1+ MPCs could be detected in synovial surface projections. Single STRO-1+ cells that co-expressed alpha-SMA resemble a population of pericyte precursors required to stabilize the immature vasculature. The second STRO-1+ cell population that was found lacked alpha-SMA but co-expressed CD34 on their surface with low intensity.
  
  
  CONCLUSIONS
  Here we can show that in the synovial tissue of patients with OA twice as many STRO-1+ MPCs can be found in synovial surface projections as compared to the sublining area. These cells are preferentially located at the basis and in the protruding end of the synovial surface projection.
authors.affiliation: Department of Orthopaedics, Medical University of Vienna, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Giurea
authors.forename: B M
authors.initials: BM
authors.lastname: Rüger
authors.forename: D
authors.initials: D
authors.lastname: Hollemann
authors.forename: G
authors.initials: G
authors.lastname: Yanagida
authors.forename: R
authors.initials: R
authors.lastname: Kotz
authors.forename: M B
authors.initials: MB
authors.lastname: Fischer
mesh_terms: D000199:Actins / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D015415:Biomarkers / Q000032:analysis*; D016022:Case-Control Studies; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000473:pathology*; D018613:Microscopy, Confocal; D010003:Osteoarthritis / Q000473:pathology*; D015207:Osteoarthritis, Hip / Q000473:pathology; D020370:Osteoarthritis, Knee / Q000473:pathology; D013583:Synovial Membrane / Q000473:pathology*
chemical_list: D000199:Actins; D018952:Antigens, CD34; D015415:Biomarkers