Immuno-magnetic separation and agar layer methods for the isolation of freeze-injured Yersinia enterocolitica O:8 from water.
To develop an effective method to isolate an injured pathogenic Yersinia enterocolitica O:8 organism from environmental samples, we compared the isolation of freeze-injured and non-injured Y. enterocolitica O:8 and found that the isolation was more successful when immuno-magnetic separation (IMS) with anti-Y. enterocolitica O:8 antibody was used. Plating onto cefsulodin-irgasan-novobiocin (CIN) agar and Virulent Yersinia enterocolitica (VYE) agar by means of the agar layer method was found to be effective in isolating the injured cells. The alkali treatment which is generally used for selective detection of Yersinia organism failed to isolate freeze-injured pathogenic Y. enterocolitica O:8 cells. Recovery methods without using the alkali treatment were superior for detecting freeze-injured Y. enterocolitica O:8. Our results demonstrate that the IMS and the agar layer methods should be used to isolate injured pathogenic Yersinia organisms from environmental samples such as water.
Koujitani
Eiko
E
Division of Animal Life Science, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Horisaka
Tomoko
T
Nomura
Yoshihiro
Y
Hara-Kudo
Yukiko
Y
Okatani
Alexandre Tomomitsu
AT
Iwata
Taketoshi
T
Kumagai
Susumu
S
Hayashidani
Hideki
H
D015169:Colony Count, Microbial; D005615:Freezing; D018189:Immunomagnetic Separation / Q000379:methods*; D019081:O Antigens / Q000737:chemistry; D011634:Public Health; D045483:Rivers / Q000382:microbiology; D014871:Water Microbiology; D015008:Yersinia enterocolitica / Q000302:isolation & purification*
D019081:O Antigens