Molecular response of leukemia HL-60 cells to genistein treatment, a proteomics study.
Genistein (GEN) is a natural protein tyrosine kinase inhibitor. We analyzed the molecular response of HL-60 cells to GEN treatment by gel-based proteomics approach. Fourteen differentially expressed proteins which are functionally involved in metabolism, cell signaling, RNA processing, cell proliferation and motility, and chaperones were identified. Both the dose- and time-dependent up-regulation of Hsp70 protein 8 and heterogeneous nuclear ribonucleoprotein (hnRNP) H1, and the down-regulation of Rab14, hnRNP C and stathmin-1 by GEN were verified by immunoblot analysis. Our novel findings provide insightful clues to the potential therapeutic targets for leukemia treatment in diverse tyrosine kinase-dependent cellular pathways.
Zhang
Daohai
D
Department of Laboratory Medicine, National University Hospital, 5 Lower Kent Ridge, Singapore 119074, Singapore.
Tai
Yan-Chin
YC
Wong
Ching-Ho Stephen
CH
Tai
Lee-Kian
LK
Koay
Evelyn Siew-Chuan
ES
Chen
Chien-Shing
CS
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D040901:Proteomics; D015203:Reproducibility of Results; D012261:Ribonucleoproteins / Q000235:genetics / Q000302:isolation & purification; D014357:Trypsin
D016588:Anticarcinogenic Agents; D004791:Enzyme Inhibitors; D009363:Neoplasm Proteins; D012261:Ribonucleoproteins; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D014357:Trypsin