First molecular detection of Rickettsia felis in fleas from Algeria.
Fleas collected in Algeria in the district of Oran between July and September 2003 were tested by polymerase chain reaction for the presence of Rickettsia spp. DNA using primers amplifying gltA and OmpA genes. Two gltA sequences identical to those of an emerging pathogen, Rickettsia felis, were detected including i) R. felis California 2 in Ctenocephalides canis from rodents and ii) R. felis RF2125 in Archeopsylla erinacei from hedgehogs.
Bitam
Idir
I
Unité d'Entomologie Médicale, Institut Pasteur d'Algérie, Algiers, Algeria.
Parola
Philippe
P
De La Cruz
Katharina Dittmar
KD
Matsumoto
Koutaro
K
Baziz
Belkacem
B
Rolain
Jean-Marc
JM
Belkaid
Miloud
M
Raoult
Didier
D
D000462:Algeria / Q000453:epidemiology; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007303:Insect Vectors / Q000145:classification / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012282:Rickettsia Infections / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission*; D041161:Rickettsia felis / Q000235:genetics / Q000302:isolation & purification*; D012377:Rodentia / Q000469:parasitology; D005423:Siphonaptera / Q000145:classification / Q000382:microbiology*
D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C078167:OMPA outer membrane proteins