title: Induction of micronuclei and other nuclear abnormalities in mussels exposed to bisphenol A, diallyl phthalate and tetrabromodiphenyl ether-47.
abstract: Analysis of micronuclei, nuclear buds, bi-polynucleated and fragmented-apoptotic cells was performed in gills of blue mussels exposed for 3 weeks to sublethal concentrations of bisphenol A, diallyl phthalate (for the both nominal concentration 50 ppb) and to tetrabromodiphenyl ether-47 (nominal concentration 5 ppb). Fourteen specimens from each treatment and control group were used for the analysis. Our results demonstrated a significant increase in micronuclei frequency after the treatment with bisphenol A (P=0.0243), diallyl phthalate (P=0.0005) and tetrabromodiphenyl ether-47 (P<0.0001; Mann-Whitney U-test). Induction of bi-nucleated (P=0.0028), fragmented-apoptotic (P=0.0004) cells and nuclear buds (P=0.0101) was found in mussels exposed to tetrabromodiphenyl ether-47 while treatment with diallyl phthalate increased the level of fragmented-apoptotic cells (P=0.0283). Bisphenol A was the only agent that resulted only in induction of micronuclei but not any other kind of nuclear injuries.
authors.affiliation: Institute of Ecology of Vilnius University, Akademijos 2, 08412 Vilnius, Lithuania. janbar@ekoi.lt
authors.forename: Janina
authors.initials: J
authors.lastname: Barsiene
authors.forename: Janina
authors.initials: J
authors.lastname: Syvokiene
authors.forename: Anne
authors.initials: A
authors.lastname: Bjornstad
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D002467:Cell Nucleus / Q000187:drug effects*; D005880:Gills / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers; D048629:Micronuclei, Chromosome-Defective / Q000662:veterinary; D015162:Micronucleus Tests / Q000662:veterinary; D049880:Mytilus edulis / Q000187:drug effects*; D010636:Phenols / Q000633:toxicity*; D010795:Phthalic Acids / Q000633:toxicity*; D011075:Polybrominated Biphenyls / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000494:pharmacology / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D055768:Halogenated Diphenyl Ethers; D010636:Phenols; D010795:Phthalic Acids; D011075:Polybrominated Biphenyls; D014874:Water Pollutants, Chemical; C511295:2,2',4,4'-tetrabromodiphenyl ether; C049098:diallyl phthalate; C006780:bisphenol A; D000098826:Bisphenol A Compounds