Promoter motifs of olfactory receptor genes expressed in distinct topographic patterns.
Novel olfactory receptor-encoding genes that are expressed in olfactory sensory neurons arranged in a clustered pattern in the nasal epithelium, typical of the mOR262 (approved gene symbol Olfr) family, were identified. The genes share sequence motifs upstream of their transcription start sites that are highly related to those previously identified as characteristic of the mOR262 genes, suggesting that these regulatory elements may contribute to governing their unique expression pattern. Promoter analyses of genes encoding class I receptors that are expressed in the dorsal region of the epithelium revealed a different, but again common set of sequence motifs. A prominent feature of the class I gene promoters are multiple O/E-like binding sites, and O/E-type transcription factors that bind to the putative promoter region of class I OR genes were in fact identified. The findings support the concept that common elements in the promoter region of these OR genes may determine their congenic expression pattern in the epithelium.
Hoppe
Reiner
R
Institute of Physiology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany.
Breer
Heinz
H
Strotmann
Joerg
J
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D018034:Olfactory Receptor Neurons / Q000033:anatomy & histology* / Q000378:metabolism*; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D018035:Receptors, Odorant / Q000235:genetics*
D018035:Receptors, Odorant; D004247:DNA