New group-specific 16S rDNA primers for monitoring foaming mycolata during saline waste-water treatment.
Newly designed group-specific PCR primers for denaturing gradient gel electrophoresis (DGGE) were used to investigate foaming mycolata from a bioreactor treating an industrial saline waste-water. Genetic profiles on DGGE gels were different with NaCl at 1.65 and 8.24 g l(-1), demonstrating that mycolata community was affected by salinity. A semi-nested PCR strategy resulted in more bands in community genetic profiles than direct amplification. DNA sequencing of bands confirmed the efficacy of the novel primers with sequences recovered being most similar to foam producing mycolata. The new group-specific primers/DGGE approach is a new step toward a more complete understanding of functionally important groups of bacteria involved in biological treatment of waste-water.
de Azeredo
L A I
LA
Universidade Federal do Rio de Janeiro, Coppe, Brasil. lucianadeazeredo@gmail.com
da Cunha
C D
CD
Rosado
A S
AS
Macrae
A
A
Freire
D M G
DM
Mendonça-Hagler
L C S
LC
Sant'Anna
G L
GL
D039903:Actinobacteria / Q000235:genetics* / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D017931:DNA Primers / Q000235:genetics*; D017753:Ecosystem; D004591:Electrophoresis, Polyacrylamide Gel; D007220:Industrial Waste / Q000032:analysis*; D009171:Mycolic Acids / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000145:classification; D017422:Sequence Analysis, DNA / Q000379:methods; D018505:Waste Management / Q000379:methods*; D018508:Water Purification / Q000379:methods
D017931:DNA Primers; D007220:Industrial Waste; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S