title: Amperometric phenol biosensor based on laponite clay-chitosan nanocomposite matrix.
abstract: A novel strategy to fabricate an amperometric biosensor for phenol determination based on chitosan/laponite nanocomposite matrix was described. The composite film was used to immobilize PPO on the surface of a glassy carbon electrode. Chitosan was utilized to improve the analytical performance of the pure clay-modified bioelectrode. The biosensor exhibited a series of properties: good affinity to its substrate (the apparent Michaelis-Menten constant for the sensor was found to be 0.16 mM), high sensitivity (674 mA M(-1)cm(-2) for catechol) and remarkable long-term stability in storage (it retains 88% of the original activity after 60 days). In addition, optimization of the biosensor construction as well as effects of experimental variables such as pH, operating potential and temperature on the amperometric response of the sensor were discussed.
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Fan
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
authors.forename: Huaiguo
authors.initials: H
authors.lastname: Xue
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: He
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004156:Catechol Oxidase / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D000077215:Clay; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D019800:Phenol / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000538:Aluminum Silicates; D004800:Enzymes, Immobilized; D019800:Phenol; D048271:Chitosan; D004156:Catechol Oxidase; D000077215:Clay