Investigation of false-positive reactions for CBH351 maize in screening PCR analysis.
Examination for CBH351 maize was conducted by the qualitative polymerase chain reaction (PCR) method in maize grain and maize processed foods obtained in the Tokyo area. The numbers of samples possibly positive in the screening test were 7 of 22 (31.8%) for maize grain samples, 4 of 14 (28.6%) for semi-processed foods, 11 of 30 (36.7%) for canned products, 3 of 30 (10.0%) for maize snacks, 3 of 4 (75%) for tacos and 1 of 3 (33.3%) for tortillas. However, CBH351 maize was not detected in the confirmation test. Therefore, the results of the screening test were false-positive. Since the reaction might have been caused by the base sequences of the 3'-end of primers CaM03-5' and CBH02-3' used in the screening test, a new primer pair was designed. The PCR products obtained with the new primer pair TMC2-5'--TMS2-3' were specific for CBH351 and were not obtained with barley, wheat, rice, RRS, Bt11, or Event176. Thus, the new primer pair shows high specificity. CBH351 maize was detected from samples containing at least 0.05% CBH 351 maize DNA by using this primer pair.
Monma
Kimio
K
Tokyo Metropolitan Institute of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
Moriuchi
Rie
R
Sagi
Naoki
N
Ichikawa
Hisatsugu
H
Satoh
Kazue
K
Tobe
Takashi
T
Kamata
Kunihiro
K
D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D005189:False Positive Reactions; D005504:Food Analysis / Q000379:methods*; D030841:Food, Genetically Modified; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D014041:Tokyo; D003313:Zea mays / Q000235:genetics
D017931:DNA Primers; D018744:DNA, Plant