[Enhanced-real time PCR: a highly sensitive method for SARS-coronavirus detection].
An enhanced real-time polymerase chain reaction (ERT-PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-Cov) has been designed for detection of SARS-Cov with high sensitivity and easy-to-interpret results, in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. The limit of detection of the ERT-PCR method was 10(-2) higher than the standard real-time PCR assay and 10(-7) higher than conventional PCR methods. The increased sensitivity of the assay would have major benefits in screening suspected SARS patients rapidly and efficiently and may help control the spread of SARS and other infectious diseases during future outbreak.
Yu
Chang-hai
CH
Neuroscience Research Institue, Peking University, Beijing 100083, China. achy@bjmu.edu.cn
Liu
Le-ting
LT
Liu
Shuang
S
Feng
Yan-yun
YY
Wang
Chen-ran
CR
Li
Hui-li
HL
Wang
Chen
C
Han
Ji-sheng
JS
D006801:Humans; D057230:Limit of Detection; D012367:RNA, Viral / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D012367:RNA, Viral