Electrofocusing of methanolic extracts for identification of individual flavonol biomolecules in Camellia species.
An effort has been made to isolate individual catechin compounds from green tea leaves in their pure form by electrophoresis. In the present study total polyphenol extraction was carried out initially and estimated through spectrophotometric and HPLC methods. Extracted polyphenol was separated on 0.7% agarose gel and visualized at 360 nm. Fragmented individual compounds were gel eluted with methanol and confirmed as (-)-epigallocatechin (EGC), (-)-epicatechin (EC), (-)-epicatechin gallate (ECG), and (-)-epigallocatechin gallate (EGCG) by HPLC. The method developed describes a suitable method for the isolation of valuable molecules in tea.
John
K M Mariya
KM
Division of Plant Physiology, UPASI Tea Research Institute, Nirar Dam BPO, Valparai, 642 127 TN, India. johnupasi@rediffmail.com
Vijayan
Deepu
D
Rahul
P R
PR
Joshi
S D
SD
Kumar
R Raj
RR
Mandal
A K A
AK
D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D044948:Flavonols / Q000032:analysis*; D007525:Isoelectric Focusing; D000432:Methanol; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D013053:Spectrophotometry
D044948:Flavonols; D010936:Plant Extracts; D002392:Catechin; C062669:epicatechin gallate; C045651:epigallocatechin gallate; C057580:gallocatechol; D000432:Methanol