Nanoparticles of cationic amphiphilic cyclodextrins entangling anionic porphyrins as carrier-sensitizer system in photodynamic cancer therapy.
The photodynamic activity of a carrier-sensitizer system consisting of heterotopic colloidal nanoparticles (diameter 100-1000 nm) of a cationic amphiphilic cyclodextrin, heptakis(2-omega-amino-O-oligo(ethylene oxide)-6-hexylthio)-beta-CD (SC6CDNH2) encapsulating the anionic 5,10,15,20-tetrakis(4-sulfonatophenyl)-21H,23H-porphyrin (TPPS) is investigated by an interdisciplinary approach involving the combination of time-resolved absorption and emission techniques with in vitro studies on cultured tumor cells. In a range of TPPS:SC6CDNH2 molar ratios between 1:10 and 1:50 these nanoparticles preserve the photodynamic properties of the entrapped photoactive agent. In fact, the triplet state of TPPS is efficiently populated, very long-lived and, as a consequence, able to produce singlet oxygen (the essential species for the photodynamic action) with quantum yield comparable to the free TPPS. Photodynamic efficacy of the carrier/sensitizer system is proven by in vitro studies on tumor Hela cells treated with TPPS:SC6CDNH2 at different molar ratio, showing significant cells death upon illumination with visible light.
Sortino
Salvatore
S
Dipartimento di Scienze Chimiche, Università di Catania,Viale Andrea Doria 8, I-95125 Catania, Italy. ssortino@unict.it
Mazzaglia
Antonino
A
Monsù Scolaro
Luigi
L
Marino Merlo
Francesca
F
Valveri
Vincenza
V
Sciortino
Maria Teresa
MT
D003505:Cyclodextrins; D016503:Drug Delivery Systems; D006367:HeLa Cells; D006801:Humans; D049329:Nanostructures; D009369:Neoplasms / Q000188:drug therapy*; D010778:Photochemotherapy; D011166:Porphyrins; D013501:Surface-Active Agents
D003505:Cyclodextrins; D011166:Porphyrins; D013501:Surface-Active Agents