The binding of BmK IT2 on mammal and insect sodium channels by surface plasmon resonance assay.
The binding of BmK IT2 to insect and mammal sodium channels was investigated by surface plasmon resonance technique. The results showed that BmK IT2 could bind not only to cockroach neuronal membranes but also to rat cerebrocortical and hippocampal synaptosomes with distinct affinity. The binding of BmK IT2 could be competed significantly by BmK AS and BmK abT, but not by AaH II, BmK I and veratridine. Furthermore, BmK alphaIV could partially inhibit the binding of BmK IT2 to rat cerebrocortical synaptosomes and cockroach neuronal membranes, but not to rat hippocampal synaptosomes. These results suggested that BmK IT2 had diverse binding properties on the mammal and insect sodium channels.
Chai
Zhi-Fang
ZF
Graduate School of the Chinese Academy of Sciences, Shanghai Institute of Physiology, Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, PR China.
Bai
Zhan-Tao
ZT
Liu
Tong
T
Pang
Xue-Yan
XY
Ji
Yong-Hua
YH
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology; D003058:Cockroaches / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology; D007700:Kinetics; D008297:Male; D009474:Neurons / Q000166:cytology; D010455:Peptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012604:Scorpion Venoms / Q000378:metabolism* / Q000494:pharmacology; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D020349:Surface Plasmon Resonance; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
C417087:BmK IT2 scorpion toxin; D010455:Peptides; D012604:Scorpion Venoms; D015222:Sodium Channels