[Detecting the polymorphism of methylenetetrahydrofolate reductase gene by denaturing high performance liquid chromatography].
OBJECTIVE
To establish a method for detecting the polymorphism of methylenetetrahydrofolate reductase gene (MTHFR).


METHODS
The MTHFR was amplified, and the amplified products were detected by denaturing high performance liquid chromatography (DHPLC), and the amplified MTHFR was confirmed by sequencing and restriction enzyme digesting.


RESULTS
A total of 334 individuals of Han people in southern China were recruited in our study, and their polymorphisms of MTHFR were detected. The accurate rate of the DHPLC method, that was very sensitive with 100% detection rate available, was over 99%. The frequencies of CC, CT and TT genotypes were 56.9%, 38.3% and 4.8% individually, and the frequencies of T and C alleles were 23.95% and 76.05% individually.


CONCLUSIONS
The DHPLC method can detect polymorphism of MTHFR rapidly, effectively and economically. And there is the existence of different MTHFR polymorphisms in area and race.
Li
Cai-ming
CM
Department of Neurology, Huizhou Central Hospital, Huizhou, Guangdong, 516001 P.R.China.
Zhang
Cheng
C
Lu
Xi-lin
XL
Feng
Hui-yu
HY
Su
Quan-xi
QX
Zeng
Ying
Y
Zhang
Hong-lian
HL
Qiu
Shu-lian
SL
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002681:China / Q000208:ethnology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D008297:Male; D045102:Methylenetetrahydrofolate Dehydrogenase (NAD+) / Q000235:genetics; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic
D045102:Methylenetetrahydrofolate Dehydrogenase (NAD+); D042965:Methylenetetrahydrofolate Reductase (NADPH2)