title: Comparative analysis of 35S and lectin promoters in transgenic soybean tissue using an automated image acquisition system and image analysis.
abstract: Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimensional robotics table and a programmable image acquisition system, which was used to semi-continuously monitor gfp gene expression during development of transgenic soybean [Glycine max (L.) Merrill] somatic embryos. Quantitative analysis of GFP expression showed that, during somatic embryo proliferation and early development, expression of lectin-GFP was not detected. During late embryo development, expression of lectin-GFP gradually increased until the levels were similar to those of 35S-GFP. The use of an automated image collection system and image analysis facilitated the frequent monitoring and quantification of gfp gene expression on a large number of samples over an extended period of time.
authors.affiliation: Department of Horticulture and Crop Science, The Ohio State University/OARDC, Wooster, OH 44691, USA.
authors.forename: Marco Tulio
authors.initials: MT
authors.lastname: Buenrostro-Nava
authors.forename: Peter P
authors.initials: PP
authors.lastname: Ling
authors.forename: John J
authors.initials: JJ
authors.lastname: Finer
mesh_terms: D001331:Automation; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D037102:Lectins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015183:Restriction Mapping; D013025:Glycine max / Q000235:genetics*
chemical_list: D037102:Lectins