title: [Effect of gamma-aminobutyric acid B receptor on nitric oxide/nitric oxide synthase system during recurrent febrile seizures].
abstract: OBJECTIVE
  To explore the effect of gamma-aminobutyric acid B receptor (GABA(B)R)on nitric oxide (NO)/nitric oxide synthase (NOS) system during recurrent febrile seizures (FS).
  
  
  METHODS
  Sprague-Dawley rats aged 21 days were randomly divided into four groups:control group, FS group, FS + baclofen group, FS + phaclofen group. FS in rats were induced ten times in a bath of warm water,once every 2 days. The plasma level of NO was detected by the spectrophotometer; the expression of nNOS mRNA was examined by in situ hybridization; the expression of nNOS protein was observed by immunohistochemistry.
  
  
  RESULTS
  Compared with those in FS group,the plasma level of NO decreased [(19.02+/-9.31) micromol/L vs (40.03+/-9.12)micromol/L], and the expression of nNOS was down-regulated in FS + baclofen group; the plasma level of NO increased [(66.46+/-8.15) micromol/L vs (40.03+/-9.12) micromol/L] and the expression of nNOS was up-regulated in FS + phaclofen group.
  
  
  CONCLUSIONS
  GABA(B)R modulated the expression of NO/NOS system during recurrent FS.
authors.affiliation: Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Jiong
authors.initials: J
authors.lastname: Qin
authors.forename: Ding-fang
authors.initials: DF
authors.lastname: Bu
authors.forename: Zhi-xian
authors.initials: ZX
authors.lastname: Yang
authors.forename: Xing-zhi
authors.initials: XZ
authors.lastname: Chang
authors.forename: Jun-bao
authors.initials: JB
authors.lastname: Du
mesh_terms: D000818:Animals; D009569:Nitric Oxide / Q000097:blood*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000378:metabolism*; D003294:Seizures, Febrile / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D018080:Receptors, GABA-B; D009569:Nitric Oxide; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat