Kinetics of lactose fermentation using a recombinant Saccharomyces cerevisiae strain.
This work presents a multi-route, non-structural kinetic model for interpretation of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this model, the values of different metabolic pathways are calculated applying a modified Monod equation rate in which the growth rate is proportional to the concentration of a key enzyme controlling the single metabolic pathway. In this study, three main metabolic routes for S. cerevisiae are considered: oxidation of lactose, reduction of lactose (producing ethanol), and oxidation of ethanol. The main bioprocess variables determined experimentally were lactose, ethanol, biomass, and dissolved oxygen concentrations. Parameters of the proposed kinetic model were established by fitting the experimental data obtained in a small lab-scale fermentor with the initial lactose concentrations ranging from 5 g/dm3 to 50 g/dm3. A very good agreement between experimental data and simulated profiles of the main variables (lactose, ethanol, biomass, and dissolved oxygen concentrations) was achieved.
Jurascík
M
M
Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.
Guimarães
P
P
Klein
J
J
Domingues
L
L
Teixeira
J
J
Markos
J
J
D019149:Bioreactors / Q000382:microbiology*; D003198:Computer Simulation; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D024861:Genetic Enhancement / Q000379:methods; D007700:Kinetics; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics; D007785:Lactose / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; D000431:Ethanol; C021499:lactose permease; D001616:beta-Galactosidase; D007785:Lactose