title: MIB-1 and MCM-2 immunohistochemical analysis does not aid in identification of serrated colorectal polyps with abnormal proliferation.
abstract: We investigated the staining characteristics of serrated polyps with abnormal proliferation (SPAP) using MIB-1 and MCM-2 to determine if they could provide assistance in delineating SPAPs from traditional hyperplastic polyps (HPs). Using published morphologic criteria we reviewed H&E slides of 107 polyps from 80 patients. Thirty-nine (36.4%) polyps met the criteria for SPAP Within a given region, polyps in the transverse colon had the largest percentage of SPAPs (50.0%) followed by the right colon (40.9%). The majority of SPAPs (82.1%) and HPs (72.1%) showed MIB-1 staining confined to the basal third of the crypts. The majority of SPAPs (59.0%) and HPs (52.9%) showed MCM-2 staining extending into the apical third of the crypts. We do not recommend MIB-1 or MCM-2 staining to differentiate SPAPs from conventional HPs, since staining characteristics are not significantly different between the 2 groups, and frequent variable crypt staining within a given polyp is difficult to interpret.
authors.affiliation: Department of Pathology, University of Vermont College of Medicine, Burlington 05405, USA.
authors.forename: David
authors.initials: D
authors.lastname: Gray
authors.forename: Ellen C
authors.initials: EC
authors.lastname: Obermann
authors.forename: Mark
authors.initials: M
authors.lastname: Evans
authors.forename: Arndt
authors.initials: A
authors.lastname: Hartmann
authors.forename: Kumarasen
authors.initials: K
authors.lastname: Cooper
authors.forename: Hagen
authors.initials: H
authors.lastname: Blaszyk
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D018797:Cell Cycle Proteins / Q000032:analysis*; D049109:Cell Proliferation; D003111:Colonic Polyps / Q000737:chemistry / Q000145:classification / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000145:classification / Q000473:pathology*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000737:chemistry / Q000473:pathology; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2; D009687:Nuclear Proteins / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2