title: A study on the molecular mechanism of resistance to amicarthiazol in Xanthomonas campestris pv. citri.
abstract: Three amicarthiazol-resistant mutants (Xuv10, Xuv20 and Xuv40) were obtained by UV induction and used in this study. Minimal inhibition concentrations (MICs) of amicarthiazol against the growth of mutants and wild-type isolate were 400 and 100 microg ml(-1) respectively. Inhibition by amicarthiazol of succinate dehydrogenase (SDH) activities of Xanthomonas campestris pv. citri (Hasse) Dye wild-type isolate (Xcc) and three resistant mutants derived from this isolate were assayed using triphenyltetrazolium chloride (TTC). The SDH activities of these mutants were significantly lower than that of Xcc. The complete nucleotide sequences of four subunits (SdhA, SdhB, SdhC and SdhD) of succinate-ubiquinone oxidoreductase (SQR) were cloned by polymerase chain reaction (PCR) amplification. An amino acid mutation (His229--> Leu229) in sdhB was found to confer resistance of X. campestris pv. citri to amicarthiazol. It is suggested that this mutation alters the SDH complex in some way that prevents binding of amicarthiazol.
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Mingguo
authors.initials: M
authors.lastname: Zhou
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.forename: Changjun
authors.initials: C
authors.lastname: Chen
authors.forename: Jianxing
authors.initials: J
authors.lastname: Wang
authors.forename: Yujun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D019943:Amino Acid Substitution; D000813:Anilides; D000900:Anti-Bacterial Agents; D002957:Citrus / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D042963:Electron Transport Complex II / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013844:Thiazoles; D016959:Xanthomonas campestris / Q000235:genetics*
chemical_list: D000813:Anilides; D000900:Anti-Bacterial Agents; D013844:Thiazoles; C511341:amicarthiazol; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase