An evaluation of cytosolic erythrocyte carbonic anhydrase and catalase in carcinoma patients: an elevation of carbonic anhydrase activity.
OBJECTIVE
The antioxidant enzyme catalase and the CO2/HCO3- exchange enzyme carbonic anhydrase are both present in significant amounts in the cytosol of erythrocytes. The aim of the present study was to investigate whether these erythrocyte enzyme activities are altered in patients who have carcinoma.


METHODS
Cytosolic erythrocyte enzyme activities were measured in 108 cancer patients presenting with carcinoma at one of variable sites, prior to clinical treatment. These were compared with an age- and sex-matched control group of 31 healthy volunteers.


RESULTS
Mean (+/-SD) catalase activities did not differ significantly, i.e. 0.0035 (+/-0.0015) EU/ml in carcinoma patients vs. 0.0031 (+/-0.00075) EU/ml in controls. However, mean carbonic anhydrase activities of 204 (+/-91) EU/ml in the carcinoma patients were significantly higher than the 158 (+/-35) EU/ml in controls (P value of 0.0065).


CONCLUSIONS
Cytosolic erythrocyte carbonic anhydrase levels may warrant further investigation as a potential peripheral marker in cancer.
Ozensoy
Ozen
O
Balikesir University Faculty of Science and Literature, Department of Chemistry, Soma Street, 10100 Balikesir, Turkey. ozensoy@balikesir.edu.tr
Kockar
Feray
F
Arslan
Oktay
O
Isik
Semra
S
Supuran
Claudiu T
CT
Lyon
Malcolm
M
D000328:Adult; D002256:Carbonic Anhydrases / Q000097:blood*; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood*; D003600:Cytosol / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000097:blood / Q000201:enzymology*
D002374:Catalase; D002256:Carbonic Anhydrases