title: Development of an HPLC method for the determination of nifedipine in human plasma by solid-phase extraction.
abstract: Nifedipine, a dihydropyridine calcium channel antagonist, is widely used in the treatment of hypertension and other cardiovascular disorders. A selective, sensitive and accurate high-performance liquid chromatographic method has been developed, validated and applied for determination of nifedipine in human plasma samples. A series of studies were conducted in order to investigate the effects of mobile phase composition, buffer concentration, mobile phase pH and concentration of organic modifiers, and to develop a convenient and easy-to-use method for quantitative analysis of nifedipine. The method involves solid-phase extraction on C18 cartridges. The chromatographic separation was accomplished on a Lichrocart Lichrospher 60 RP selectB column with a mobile phase composed of 0.020 mol/L KH2PO4 (pH 4.8) and acetonitrile (42:58, v/v). UV detection was set at 240 nm. The calibration curve was linear in the concentration range of 5.0-200.0 ng/mL for nifedipine in plasma and the limit of quantification was 5.0 ng/mL.
authors.affiliation: Institute of Preclinical and Clinical Pharmacology and Toxicology, St. Cyril and Methodius University, Medical Faculty, 50 Divizija bb, 1000 Skopje, Macedonia. pharma@on.net.mk
authors.forename: Dragica
authors.initials: D
authors.lastname: Zendelovska
authors.forename: Suzana
authors.initials: S
authors.lastname: Simeska
authors.forename: Olgica
authors.initials: O
authors.lastname: Sibinovska
authors.forename: Elena
authors.initials: E
authors.lastname: Kostova
authors.forename: Kalina
authors.initials: K
authors.lastname: Milosevska
authors.forename: Krume
authors.initials: K
authors.lastname: Jakovski
authors.forename: Emilija
authors.initials: E
authors.lastname: Jovanovska
authors.forename: Igor
authors.initials: I
authors.lastname: Kikerkov
authors.forename: Jasmina
authors.initials: J
authors.lastname: Trojacanec
authors.forename: Dimce
authors.initials: D
authors.lastname: Zafirov
mesh_terms: D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009543:Nifedipine / Q000097:blood* / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D009543:Nifedipine