title: Physiological changes in Biomphalaria glabrata Say, 1818 (Pulmonata: Planorbidae) caused by sub-lethal concentrations of the latex of Euphorbia splendens var. hislopii N.E.B (Euphorbiaceae).
abstract: Molluscides have been used as one of the strategies to control schistosomiasis. Many plant extracts with molluscidal effects have been tested, but the action of the latex of Euphorbia splendens var. hislopii is considered the most promising because it meets the recommendations of the World Health Organization (WHO). The objective of this study was to determine the lethal dose and identify the effects of the different doses of latex of E. splendens var. hislopii on the physiology of Biomphalaria glabrata submitted to treatment for 24 h. The concentrations of glucose, uric acid and total proteins in the hemolymph and of glycogen in the digestive gland and cephalopodal mass were determined. The LD50 value was 1 mg/l. The highest escape index was found to be at a concentration of 0.6 mg/l. The results showed that the latex of E. splendens var. hislopii caused a sharp reduction in the reserves of glycogen in the digestive gland and elevation of the protein content in the hemolymph of B. glabrata.
authors.affiliation: Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, 23890-000 Seropédica, RJ, Brazil.
authors.forename: Clélia Christina
authors.initials: CC
authors.lastname: Mello-Silva
authors.forename: Maurício Carvalho de
authors.initials: MC
authors.lastname: Vasconcellos
authors.forename: Jairo
authors.initials: J
authors.lastname: Pinheiro
authors.forename: Maria de Lurdes de Azevedo
authors.initials: Mde L
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000187:drug effects* / Q000502:physiology; D028481:Euphorbia / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D006003:Glycogen / Q000032:analysis; D006458:Hemolymph / Q000737:chemistry; D007840:Latex / Q000302:isolation & purification / Q000494:pharmacology*; D007928:Lethal Dose 50; D010936:Plant Extracts / Q000494:pharmacology; D011506:Proteins / Q000032:analysis; D014527:Uric Acid / Q000032:analysis
chemical_list: D007840:Latex; D010936:Plant Extracts; D011506:Proteins; D014527:Uric Acid; D006003:Glycogen; D005947:Glucose