title: D-Galactose induces cellulase gene expression in Hypocrea jecorina at low growth rates.
abstract: Lactose (1,4-O-beta-d-galactopyranosyl-d-glucose) is a soluble and economic carbon source for the industrial production of cellulases or recombinant proteins by Hypocrea jecorina (anamorph Trichoderma reesei). The mechanism by which lactose induces cellulase formation is not understood. Recent data showed that the galactokinase step is essential for cellulase induction by lactose, but growth on d-galactose alone does not induce cellulases. Consequently, the hypothesis was tested that d-galactose may be an inducer only at a low growth rate, which is typically observed when growing on lactose. Carbon-limited chemostat cultivations of H. jecorina were therefore performed at different dilution rates with d-galactose, lactose, galactitol and d-glucose. Cellulase gene expression was monitored by using a strain carrying a fusion between the cbh2 (encoding cellobiohydrolase 2, Cel6A) promoter region and the Aspergillus niger glucose oxidase gene and by identification of the two major cellobiohydrolases Cel7A and Cel6A. The results show that d-galactose indeed induces cbh2 gene transcription and leads to Cel7A and Cel6A accumulation at a low (D=0.015 h(-1)) but not at higher dilution rates. At the same dilution rate, growth on d-glucose did not lead to cbh2 promoter activation or Cel6A formation but a basal level, lower than that observed on d-galactose, was detected for the carbon-catabolite-derepressible Cel7A. Lactose induced significantly higher cellulase levels at 0.015 h(-1) than d-galactose and induced cellulases even at growth rates up to 0.042 h(-1). Results of chemostats with an equimolar mixture of d-galactose and d-glucose essentially mimicked the behaviour on d-galactose alone, whereas an equimolar mixture of d-galactose and galactitol, the first intermediate of a recently described second pathway of d-galactose catabolism, led to cellulase induction at D=0.030 h(-1). It is concluded that d-galactose indeed induces cellulases at low growth rate and that the operation of the alternative pathway further increases this induction. However, under those conditions lactose is still a superior inducer for which the mechanism remains to be clarified.
authors.affiliation: Department of Microbiology and Biotechnology, Faculty of Sciences, University of Debrecen, H-4010, PO Box 63, Debrecen, Hungary.
authors.forename: Levente
authors.initials: L
authors.lastname: Karaffa
authors.affiliation: Department of Microbiology and Biotechnology, Faculty of Sciences, University of Debrecen, H-4010, PO Box 63, Debrecen, Hungary.
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Fekete
authors.affiliation: Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9/1665, A-1060 Wien, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Gamauf
authors.affiliation: Department of Microbiology and Biotechnology, Faculty of Sciences, University of Debrecen, H-4010, PO Box 63, Debrecen, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Szentirmai
authors.affiliation: Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9/1665, A-1060 Wien, Austria.
authors.forename: Christian P
authors.initials: CP
authors.lastname: Kubicek
authors.affiliation: Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9/1665, A-1060 Wien, Austria.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Seiboth
mesh_terms: D019677:Artificial Gene Fusion; D015153:Blotting, Western; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000032:analysis / Q000235:genetics; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D004376:Galactitol / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D035901:Hypocrea / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D007785:Lactose / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D004376:Galactitol; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; D005947:Glucose; D007785:Lactose; D005690:Galactose