title: Rapid bidirectional allele-specific PCR identification for triazine resistance in higher plants.
abstract: Triazine resistance is reported to be due to chloroplast herbicide target insensitivity in most species, and this is most often caused by a Ser(264)-Gly mutation at the D1 protein. In order to ascertain whether this mutation is really predominant amongst resistant plants, and also for gene flow studies, a rapid test is needed that allows the testing of large quantities of plants. Here a bidirectional allele-specific PCR (polymerase chain reaction) identification is proposed. The designed primers were shown to be universal in the three grass and three broadleaf species examined.
authors.affiliation: Unité Mixte de Recherche Biologie et Gestion des Adventices, INRA, BP 86510, F-21065 Dijon, France.
authors.forename: Xin
authors.initials: X
authors.lastname: Tian
authors.forename: Henri
authors.initials: H
authors.lastname: Darmency
mesh_terms: D000483:Alleles; D001483:Base Sequence; D004252:DNA Mutational Analysis; D017931:DNA Primers; D004351:Drug Resistance / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006540:Herbicides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D045332:Photosystem II Protein Complex / Q000235:genetics*; D006109:Poaceae; D016133:Polymerase Chain Reaction / Q000379:methods*; D014227:Triazines / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D006540:Herbicides; D045332:Photosystem II Protein Complex; D014227:Triazines