A mammalian organelle map by protein correlation profiling.
Protein localization to membrane-enclosed organelles is a central feature of cellular organization. Using protein correlation profiling, we have mapped 1,404 proteins to ten subcellular locations in mouse liver, and these correspond with enzymatic assays, marker protein profiles, and confocal microscopy. These localizations allowed assessment of the specificity in published organellar proteomic inventories and demonstrate multiple locations for 39% of all organellar proteins. Integration of proteomic and genomic data enabled us to identify networks of coexpressed genes, cis-regulatory motifs, and putative transcriptional regulators involved in organelle biogenesis. Our analysis ties biochemistry, cell biology, and genomics into a common framework for organelle analysis.
Foster
Leonard J
LJ
Center for Experimental BioInformatics (CEBI), Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
de Hoog
Carmen L
CL
Zhang
Yanling
Y
Zhang
Yong
Y
Xie
Xiaohui
X
Mootha
Vamsi K
VK
Mann
Matthias
M
D000818:Animals; D002499:Centrifugation, Density Gradient; D016000:Cluster Analysis; D030562:Databases, Protein; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D023281:Genomics; D008099:Liver / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D015388:Organelles / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D021381:Protein Transport; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D040901:Proteomics; D014157:Transcription Factors / Q000378:metabolism
D011506:Proteins; D014157:Transcription Factors