title: [Gamma-aminobutyric acid B receptor regulates the expression of hydrogen sulfide/cystathionine-beta-synthase system in recurrent febrile seizures].
abstract: OBJECTIVE
  Febrile seizure (FS) is one of the most common seizure types in children. Our previous studies have demonstrated that both gamma-aminobutyric acid B receptor (GABABR) and hydrogen sulfide (H2S) are involved in the pathogenesis of FS. This study was designed to explore the effect of GABABR on H2S/cystathionine-beta-synthase (CBS) system in recurrent FS.
  
  
  METHODS
  Sixty-four Sprague-Dawley rats aged 21 days were randomly assigned into four groups: Control (37 degrees C water bath exposure), FS, FS+baclofen (GABABR excitomotor), and FS+phaclofen (GABABR inhibitor) groups (n=16 each). FS was induced by warm water bath exposure (45.2 degrees C, once every 2 days, 10 times in total. The plasma level of H2S was detected by the spectrophotometer. The expression of CBS mRNA was examined by in situ hybridization. The expressions of CBS protein was observed by immunohistochemistry.
  
  
  RESULTS
  The plasma level of H2S increased in the FS+baclofen group (427.45 +/- 15.91 micromol/L) but decreased in the FS+phaclofen group (189.72 +/- 21.53 micromol/L) compared with that in the FS group (362.14 +/- 19.71 micromol/L). The expressions of CBS mRNA and protein were up-regulated in the FS+baclofen group but were down-regulated in the FS+phaclofen group compared with those in the FS group.
  
  
  CONCLUSIONS
  GABABR modulated the expression of H2S/CBS system in recurrent FS.
authors.affiliation: Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Jiong
authors.initials: J
authors.lastname: Qin
authors.forename: Ding-Fang
authors.initials: DF
authors.lastname: Bu
authors.forename: Xing-Zhi
authors.initials: XZ
authors.lastname: Chang
authors.forename: Zhi-Xian
authors.initials: ZX
authors.lastname: Yang
authors.forename: Jun-Bao
authors.initials: JB
authors.lastname: Du
mesh_terms: D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D003541:Cystathionine beta-Synthase / Q000235:genetics / Q000502:physiology*; D006862:Hydrogen Sulfide / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000502:physiology*; D012008:Recurrence; D003294:Seizures, Febrile / Q000378:metabolism*
chemical_list: D018080:Receptors, GABA-B; D003541:Cystathionine beta-Synthase; D001418:Baclofen; D006862:Hydrogen Sulfide