Expression profiling of auxin-treated Arabidopsis roots: toward a molecular analysis of lateral root emergence.
Treating Arabidopsis roots with exogenous auxin results in dramatic changes in cellular processes including de novo induction of lateral roots which later emerge through the overlying cells. Microarray experiments reveal approximately 80 genes that are substantially up-regulated in the root over the first 12 h following auxin treatment. We hypothesize that the observed increase in expression of pectate lyase family genes leads to degradation of the pectin-rich middle lamellae, allowing cells in the parent root to separate cleanly. Differences in the degree of pectin methylation in lateral and parent roots may explain why lateral roots are not degraded themselves.
Laskowski
Marta
M
Biology Department, Oberlin College, Oberlin, OH 44074, USA. mlaskows@oberlin.edu
Biller
Steven
S
Stanley
Ken
K
Kajstura
Tymoteusz
T
Prusty
Reeta
R
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D002473:Cell Wall / Q000187:drug effects / Q000502:physiology; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008745:Methylation; D020411:Oligonucleotide Array Sequence Analysis; D010741:Phospholipases A / Q000032:analysis / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*; D011133:Polysaccharide-Lyases / Q000032:analysis / Q000235:genetics / Q000502:physiology
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D007210:Indoleacetic Acids; D010741:Phospholipases A; D011133:Polysaccharide-Lyases; C468914:AT1G67750 protein, Arabidopsis; C513206:PLA2 protein, Arabidopsis; C023911:pectate lyase