title: Modifications of trout (Oncorhynchus mykiss) muscle proteins by preslaughter activity.
abstract: The effect of two different preslaughter procedures (limited or 15-min intense muscular activity) on muscle trout proteins was investigated. Muscle was sampled 45 min and 24 h post-mortem, proteins were separated using two-dimensional electrophoresis, and spots of interest were tentatively identified by MALDI-TOF spectrometry. Twenty-nine and 4 spots were differentially represented between the two groups of fish at 45 min and 24 h post-mortem, respectively. Spots that could be identified corresponded mainly to proteins involved in energy-producing pathways (triosephosphate isomerase, enolase, pyruvate dehydrogenase) or to structural proteins (desmin, cap-Z, myosin heavy chain fragment). Persistent under-representation of desmin, a key cytoskeletal protein, in fish submitted to intense muscular activity suggests that such a preslaughter treatment can have an effect on post-mortem muscle integrity.
authors.affiliation: Quality of Animal Products, INRA, 63122 St Genès Champanelle, France. morzel@clermont.inra.fr
authors.forename: Martine
authors.initials: M
authors.lastname: Morzel
authors.forename: Christophe
authors.initials: C
authors.lastname: Chambon
authors.forename: Florence
authors.initials: F
authors.lastname: Lefèvre
authors.forename: Gilles
authors.initials: G
authors.lastname: Paboeuf
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Laville
mesh_terms: D000818:Animals; D051347:CapZ Actin Capping Protein / Q000032:analysis; D003893:Desmin / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005511:Food Handling / Q000379:methods*; D009124:Muscle Proteins / Q000032:analysis*; D018995:Myosin Heavy Chains / Q000032:analysis; D017686:Oncorhynchus mykiss; D011180:Postmortem Changes; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D051347:CapZ Actin Capping Protein; D003893:Desmin; D009124:Muscle Proteins; D018995:Myosin Heavy Chains