title: A lag-phase in the reduction of flavin dependent thymidylate synthase (FDTS) revealed a mechanistic missing link.
abstract: An unexpected substrate-dependent lag-phase, found in the single turnover reduction of FDTS bound flavin, sheds light on the molecular mechanism of this alternative thymidylate synthase.
authors.affiliation: Department of Chemistry, University of Iowa, Iowa City, IA, USA. amnon-kohen@uiowa.edu
authors.forename: Aaron
authors.initials: A
authors.lastname: Mason
authors.forename: Nitish
authors.initials: N
authors.lastname: Agrawal
authors.forename: M Todd
authors.initials: MT
authors.lastname: Washington
authors.forename: Scott A
authors.initials: SA
authors.lastname: Lesley
authors.forename: Amnon
authors.initials: A
authors.lastname: Kohen
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D003856:Deoxyuracil Nucleotides / Q000737:chemistry / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D009249:NADP / Q000737:chemistry / Q000378:metabolism; D020124:Thermotoga maritima / Q000201:enzymology*; D013940:Thymidylate Synthase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003856:Deoxyuracil Nucleotides; D005182:Flavin-Adenine Dinucleotide; D009249:NADP; C007267:2'-deoxyuridylic acid; D013940:Thymidylate Synthase