A preparative isolation and purification of arctigenin and matairesinol from Forsythia koreana by centrifugal partition chromatography.
Centrifugal partition chromatography was applied to separate arctigenin and matairesinol from Forsythia koreana extract with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5:5:5:5 v/v). Using this method, arctigenin and matairesinol were successfully separated from partially purified F. koreana extracts in only one step. The purities of isolated compounds were determined to be over 90% by HPLC analysis.
Kim
Chul Young
CY
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
Ahn
Mi-Jeong
MJ
Kim
Jinwoong
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D031657:Forsythia / Q000737:chemistry*; D005663:Furans / Q000032:analysis / Q000302:isolation & purification*; D017705:Lignans / Q000032:analysis / Q000302:isolation & purification*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
D005663:Furans; D017705:Lignans; D010936:Plant Extracts; D012997:Solvents; C068935:matairesinol; C071942:arctigenin