title: Thrombospondin 1 mRNA as a candidate for a marker to detect thyroid-derived fibroblasts in fine needle aspiration biopsy of the thyroid.
abstract: Possible interference of fibroblasts is suggested in aspiration biopsy nucleic acid diagnosis (ABND). However, detection of fibroblasts in the aspirates is difficult, because the gene expression profiles of thyroid malignancies and fibroblasts are much alike in many aspects. To identify a specific marker for thyroid-derived fibroblasts, the data of the serial analysis of gene expression (SAGE) were compared to screen differentially expressed genes between fibroblasts and thyroid normal and tumor tissues. In the SAGE data, 5 genes were identified to be differentially expressed. Among these, thrombospondin 1 (TSP-1, THBS1) mRNA was the most differentially expressed. Further, the overexpression of TSP-1 mRNA in fibroblasts was confirmed by real-time RT-PCR analysis using 73 thyroid normal and tumor tissues and 7 cultures of thyroid-derived fibroblasts. These results suggest that TSP-1 mRNA is a possible marker for contamination of thyroid-derived fibroblasts.
authors.affiliation: Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Takano
authors.forename: Akira
authors.initials: A
authors.lastname: Miyauchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Kanji
authors.initials: K
authors.lastname: Kuma
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Amino
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D044963:Biopsy, Fine-Needle; D002478:Cells, Cultured; D005240:Feasibility Studies; D005347:Fibroblasts / Q000378:metabolism*; D018628:Gene Dosage; D020869:Gene Expression Profiling; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019700:Thrombospondin 1 / Q000032:analysis / Q000235:genetics*; D013959:Thyroid Diseases / Q000175:diagnosis; D013961:Thyroid Gland / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D019700:Thrombospondin 1