Sensitivity enhancement of spectral surface plasmon resonance biosensors for the analysis of protein arrays.
A novel method for sensitivity enhancement of spectral surface plasmon resonance (SPR) biosensors was presented by reducing the refractive index of the sensing prism in the analysis of protein arrays. Sensitivity of spectral SPR biosensors with two different prisms (BK-7, fused silica) was analyzed by net shifts of resonance wavelength for specific interactions of GST-GTPase binding domain of p21-activated kinase-1 and anti-GST on a mixed thiol surface. Sensitivity was modulated by the refractive index of the sensing prism of the spectral SPR biosensors with the same incidence angle. The sensitivity of a spectral SPR biosensor with a fused silica prism was 1.6 times higher than that with a BK-7 prism at the same incidence angle of 46.2 degrees. This result was interpreted by increment of the penetration depth correlated with evanescent field intensity at the metal/dielectric interface. Therefore, it is suggested that sensitivity enhancement is readily achieved by reducing the refractive index of the sensing prism of spectral SPR biosensors to be operated at long wavelength ranges for the analysis of protein arrays.
Yuk
Jong Seol
JS
Department of Molecular and Cellular Biochemistry and Nano-Bio Sensor Research Center, Kangwon National University School of Medicine, Chunchon, Kangwon-Do, 200-701, South Korea.
Hong
Duk-Geun
DG
Jung
Jae-Wan
JW
Jung
Se-Hui
SH
Kim
Hyun-Soo
HS
Han
Jeong-A
JA
Kim
Young-Myeong
YM
Ha
Kwon-Soo
KS
D001703:Biophysics / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods*; D018625:Microscopy, Atomic Force; D040081:Protein Array Analysis / Q000379:methods*; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D054462:p21-Activated Kinases
D017346:Protein Serine-Threonine Kinases; D054462:p21-Activated Kinases