title: Direct interaction of post-synaptic density-95/Dlg/ZO-1 domain-containing synaptic molecule Shank3 with GluR1 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor.
abstract: A class of scaffolding protein containing the post-synaptic density-95/Dlg/ZO-1 (PDZ) domain is thought to be involved in synaptic trafficking of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors during development. To clarify the molecular mechanism of AMPA receptor trafficking, we performed a yeast two-hybrid screening system using the cytoplasmic tail of the GluR1 subunit of AMPA receptor as a bait and identified a synaptic molecule, Shank3/ProSAP2, as a GluR1 subunit-interacting molecule. Shank3 is a PDZ domain-containing multidomain protein and is predominantly expressed in developing neurons. Using the glutathione S-transferase pull-down assay and immunoprecipitation technique we demonstrated that the GluR1 subunit directly binds to the PDZ domain of Shank3 via its carboxyl terminal PDZ-binding motif. We raised anti-Shank3 antibody to investigate the expression of Shank3 in cortical neurons. The pattern of Shank3 immunoreactivity was strikingly punctate, mainly observed in the spines, and closely matched the pattern of post-synaptic density-95 immunoreactivity, indicating that Shank3 is colocalized with post-synaptic density-95 in the same spines. When Shank3 and the GluR1 subunit were overexpressed in primary cortical neurons, they were also colocalized in the spines. Taken together with the biochemical interaction of Shank3 with the GluR1 subunit, these results suggest that Shank3 is an important molecule that interacts with GluR1 AMPA receptor at synaptic sites of developing neurons.
authors.affiliation: Department of Neurochemistry, National Institute of Neuroscience, Kodaira, Tokyo, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Uchino
authors.forename: Hidenori
authors.initials: H
authors.lastname: Wada
authors.forename: Shizuyo
authors.initials: S
authors.lastname: Honda
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Ondo
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Uchiyama
authors.forename: Moe
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: Eri
authors.initials: E
authors.lastname: Suzuki
authors.forename: Takae
authors.initials: T
authors.lastname: Hirasawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kohsaka
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000502:physiology; D016466:CHO Cells; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism* / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D006224:Cricetinae; D049229:Dendritic Spines / Q000378:metabolism / Q000648:ultrastructure; D000076122:Disks Large Homolog 4 Protein; D018698:Glutamic Acid / Q000378:metabolism; D051528:Guanylate Kinases; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism* / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D002352:Carrier Proteins; D000076122:Disks Large Homolog 4 Protein; D018698:Glutamic Acid; D051528:Guanylate Kinases; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D018091:Receptors, AMPA; C501447:Dlg4 protein, mouse; C492356:Shank3 protein, mouse