title: Bilayer fibril formation by genetically engineered polypeptides: preparation and characterization.
abstract: A de novo, genetically engineered 687 residue polypeptide expressed in E. coli has been found to form highly rectilinear, beta-sheet containing fibrillar structures. Tapping-mode atomic force microscopy, deep-UV Raman spectroscopy, and transmission electron microscopy definitively established the tendency of the fibrils to predominantly display an apparently planar bilayer or ribbon assemblage. The ordered self-assembly of designed, extremely repetitive, high molecular weight peptides is a harbinger of the utility of similar materials in nanoscience and engineering applications.
authors.affiliation: Department of Chemistry and College of Nanoscale Science and Engineering, The University at Albany, State University of New York, Albany, New York 12222, USA.
authors.forename: Natalya I
authors.initials: NI
authors.lastname: Topilina
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Higashiya
authors.forename: Narender
authors.initials: N
authors.lastname: Rana
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Ermolenkov
authors.forename: Christopher
authors.initials: C
authors.lastname: Kossow
authors.forename: Autumn
authors.initials: A
authors.lastname: Carlsen
authors.forename: Silvana C
authors.initials: SC
authors.lastname: Ngo
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Wells
authors.forename: Eric T
authors.initials: ET
authors.lastname: Eisenbraun
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Dunn
authors.forename: Igor K
authors.initials: IK
authors.lastname: Lednev
authors.forename: Robert E
authors.initials: RE
authors.lastname: Geer
authors.forename: Alain E
authors.initials: AE
authors.lastname: Kaloyeros
authors.forename: John T
authors.initials: JT
authors.lastname: Welch
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D008051:Lipid Bilayers / Q000138:chemical synthesis* / Q000737:chemistry* / Q000302:isolation & purification; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010316:Particle Size; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D015202:Protein Engineering; D017433:Protein Structure, Secondary; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman
chemical_list: D008051:Lipid Bilayers; D010455:Peptides; D004247:DNA