1H NMR spectroscopy for the prediction of therapeutic outcome in patients with fulminant hepatic failure.
A high-resolution (1)H NMR study of serum and urine of fulminant hepatic failure patients (n = 22) [surviving (n = 12) and non-surviving (n = 10)] is reported. Glutamine in serum and urine glutamine:creatinine ratio were higher in non-surviving patients compared with surviving patients [serum glutamine, 3.08 (1.68-7.11) vs 0.56 (0.34-0.99) mM, median and range; p = 0.0001 and urine glutamine:creatinine ratio, 1.72 (0.24-7.76) vs 0.39 (0.1-0.84), p = 0.1], and urine urea:creatinine ratio was higher in surviving patients compared with non-surviving patients [10.83 (0.2-22.6) vs 2.09 (0.96-4.0), p = 0.002]. On the other hand, no significant differences were found in the conventionally employed clinical parameters such as serum alanylaminotransferase, aspartylaminotransferase and bilirubin except prothrombin time (p = 0.02). The difference in serum glutamine and urine urea was significant in the two categories of patients and distinctly different values of serum glutamine for both the categories of patients correctly predicted the outcome. These results promise immense potential for NMR spectroscopy in rapidly deciding on the need for advanced therapeutic intervention such as artificial liver support or emergency liver transplantation in FHF.
Saxena
Varsha
V
Centre of Biomedical Magnetic Resonance, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226 014, India.
Gupta
Ashish
A
Nagana Gowda
G A
GA
Saxena
Rajan
R
Yachha
S K
SK
Khetrapal
C L
CL
D000293:Adolescent; D000328:Adult; D003404:Creatinine / Q000097:blood / Q000652:urine; D005973:Glutamine / Q000097:blood / Q000652:urine; D006801:Humans; D017114:Liver Failure, Acute / Q000097:blood / Q000628:therapy / Q000652:urine; D008875:Middle Aged; D019906:Nuclear Magnetic Resonance, Biomolecular; D011237:Predictive Value of Tests; D011379:Prognosis; D016896:Treatment Outcome
D005973:Glutamine; D003404:Creatinine