[Molecular cloning and expression analysis of a SUPERMAN-like zinc finger protein gene in upland cotton].
The zinc finger proteins belong to the largest family of regulatory transcription factors, which play an important role in growth and development in animal and plant systems. SUPERMAN-like zinc finger protein gene has only one "finger like" motif. A pair of degenerate primers was designed according to the conserved regions, and 3 kinds of EST of this family were isolated from cotton through RT-PCR. The full length of one SUPERMAN-like zinc finger protein also has been acquired. The entire coding region is 744 bp and encodes a polypeptide of 248 amino acids with 40% homology to RBE protein of Arabidopsis deposited in the GenBank. This gene was designated as GZFP. It has the conserved zinc finger domain and the leucine rich region at the carboxyl terminus but no intron in the coding region. GZFP also has the plant nuclear localization signal. GZFP shows a more expression pattern in floral buds, ovaries, petals and roots than in phloem, xylem, fibers, leaves and seeds of cotton by RT-PCR, although it has a very low detection level and there is not any homologous ESTs found in the GenBank. Analysis of the 5' flanking sequence shows there are several regulatory elements responsible for pollen and root expression, four core sites required for binding of Dof proteins and four light-regulated elements.
Yang
Yu-Wen
YW
Institute of Agrobiology Genetics and Physiolog, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. ywyang92@yahoo.com.cn
Ni
Wan-Chao
WC
Zhang
Bao-Long
BL
Shen
Xin-Lian
XL
Zhang
Xiang-Gui
XG
Xu
Ying-Jun
YJ
Yao
Shu
S
D024506:5' Flanking Region / Q000235:genetics; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D017343:Genes, Plant; D018745:Genome, Plant; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification; D020539:Sequence Analysis, Protein; D016335:Zinc Fingers / Q000235:genetics* / Q000502:physiology
D010940:Plant Proteins