title: DE-cadherin, a core component of the adherens junction complex modifies subcellular localization of the Drosophila gap junction protein innexin2.
abstract: The Drosophila innexin multigene family of gap junction encoding proteins consists of eight family members whose function in epithelial morphogenesis is mostly unknown. We have recently shown that innexin2 plays a crucial role in the organization of embryonic epithelia. Innexin2 protein accumulates in the epidermis in the apico-lateral membrane domain and colocalizes with core proteins of adherens junctions, such as DE-cadherin and Armadillo, the ss -catenin homolog. Innexin2 localization is altered in both armadillo and DE-cadherin mutants Biochemical interaction studies point to a direct interaction of DE-cadherin and Armadillo with innexin2 suggesting a close link between gap junction and adherens junction biogenesis. We have used the Drosophila Schneider cell tissue culture system to further study the interaction of innexin2 with DE-cadherin. Our results provide evidence that DE-cadherin may be a key component to control trafficking, and localization of Innexin2 to the plasma membrane.
authors.affiliation: Institute of Molecular Physiology and Developmental Biology, University of Bonn, Bonn, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Bauer
authors.forename: A
authors.initials: A
authors.lastname: Weimbs
authors.forename: H
authors.initials: H
authors.lastname: Lechner
authors.forename: M
authors.initials: M
authors.lastname: Hoch
mesh_terms: D022005:Adherens Junctions / Q000737:chemistry / Q000648:ultrastructure*; D000818:Animals; D051186:Armadillo Domain Proteins / Q000032:analysis; D015820:Cadherins / Q000502:physiology*; D051177:Catenins / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D017630:Connexins / Q000032:analysis* / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins / Q000032:analysis* / Q000378:metabolism / Q000502:physiology*; D004848:Epithelium / Q000196:embryology* / Q000378:metabolism*; D017629:Gap Junctions / Q000737:chemistry / Q000648:ultrastructure*; D014157:Transcription Factors / Q000032:analysis
chemical_list: C495305:ARM protein, Drosophila; D051186:Armadillo Domain Proteins; D015820:Cadherins; D051177:Catenins; D017630:Connexins; D029721:Drosophila Proteins; D014157:Transcription Factors; C120024:inx2 protein, Drosophila; C501889:shg protein, Drosophila