title: One-step preparation of chitosan-coated cationic liposomes by an improved supercritical reverse-phase evaporation method.
abstract: High-pressure carbon dioxide in contact with water dissolves to form carbonic acid, causing a decrease in pH. By use of these characteristics of a CO2/H2O biphasic system, chitosan-coated cationic liposomes of l-alpha-dipalmitoylphosphatidylcholine were successfully prepared by an improved supercritical reverse-phase evaporation (ISCRPE) method. Liposome-chitosan complexes carrying a positive charge were prepared in a single-step procedure without the use of acid or organic solvent, including ethanol. The maximum trapping efficiency of liposomes prepared by the ISCRPE method was 17%, with or without the addition of chitosan, compared to only 2% for liposomes prepared by the Bangham method. Furthermore, the liposomal dispersion was stable at room temperature in a sealed tube for over 30 days.
authors.affiliation: Nanotechnology Research Institute and Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan. katsuto-otake@aist.go.jp
authors.forename: Katsuto
authors.initials: K
authors.lastname: Otake
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimomura
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Goto
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Furuya
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yoda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Takebayashi
authors.forename: Hideki
authors.initials: H
authors.lastname: Sakai
authors.forename: Masahiko
authors.initials: M
authors.lastname: Abe
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002412:Cations; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems; D005614:Freeze Fracturing; D066298:In Vitro Techniques; D008081:Liposomes / Q000737:chemistry / Q000302:isolation & purification*; D008722:Methods; D010316:Particle Size
chemical_list: D002412:Cations; D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D048271:Chitosan