[Effect of new qingkailing injection on cerebral edema following intracerebral hemorrhage in rats].
OBJECTIVE
To investigate the effect of New Qingkailing injection (NQKLI) on cerebral edema following intracerebral hemorrhage (ICH) induced by collagenase VII in rats.


METHODS
After being established into ICH model by collagenase VII, rats were treated with NQKLI by intraperitoneal injection. Forty-eight hrs later, brain contents of water was detected with wet and dry method, calcium content in brain was detected by atomic spectrophotometer, tumor necrosis factor-alpha (TNF-alpha) in brain tissue was determined by liquid competitive ibhibitory immunoassay, and serum matrix metalloproteinase-9 (MMP-9) were detected with enzyme-linked immunosorbent assay (ELISA) respectively.


RESULTS
NQKLI reduced the contents of water, calcium and TNF-alpha content of brain tissue and serum MMP-9 in rats with ICH.


CONCLUSIONS
NQKLI could alleviate both vasogenic and cytotoxic cerebral edema by prohibiting calcium over-load, protecting basilar membrane and eliminating inflammation.
Liu
Min
M
College of Basic Medicine, Beijing University of Traditional Chinese Medicine, Beijing (100029).
Guo
Ming-Zhang
MZ
Li
Peng-Ying
PY
Zhang
Hanrui
H
Li
Lina
L
Li
Pengtao
P
Wang
Qingguo
Q
D000818:Animals; D001929:Brain Edema / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000150:complications*; D017364:Collagenases; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D007267:Injections; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D018696:Neuroprotective Agents / Q000008:administration & dosage; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D014409:Tumor Necrosis Factor-alpha; D017364:Collagenases; D020780:Matrix Metalloproteinase 9