Simultaneous determination of (fluoro)quinolone antibiotics in kidney, marine products, eggs, and muscle by enzyme-linked immunosorbent assay (ELISA).
A direct competitive enzyme-linked immunosorbent assay (ELISA) was developed to detect a broad range of (fluoro)quinolones in various matrices. In the optimized generic test, anti-sarafloxacin antibodies in combination with norfloxacin conjugate showed 50% binding inhibition at 0.21 ng mL(-)(1) for sarafloxacin in buffer. Screening for this class of antibiotics is accomplished using a simple, rapid extraction carried out with a 1:1 mixture of methanol and phosphate-buffered saline adjusted to pH 7.4. This common extraction was able to detect 15 (fluoro)quinolone residues such as sarafloxacin, norfloxacin, difloxacin, ciprofloxacin, pefloxacin, ofloxacin, cinoxacin, danofloxacin, enrofloxacin, marbofloxacin, lomefloxacin, enoxacin, flumequine, oxolinic acid, and nalidixic acid in pig kidney, poultry muscle, egg, fish, and shrimp. The assay's detection capabilities (CCbeta) for most of these compounds were <10 microg kg(-)(1) except for the sarafloxacin-, oxolinic acid-, flumequine-, and cinoxacin-spiked matrices, the estimated CCbeta values of which were <4, <25, <100, and <200 microg kg(-)(1), respectively.
Huet
Anne-Catherine
AC
Centre d'Economie Rurale, laboratoire d'Hormonologie Animale, rue Point du Jour 8, 6900 Marloie, Belgium.
Charlier
Caroline
C
Tittlemier
Sheryl A
SA
Singh
Gurmit
G
Benrejeb
Samuel
S
Delahaut
Philippe
P
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis; D002417:Cattle; D002645:Chickens; D004531:Eggs / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005396:Fish Products / Q000032:analysis*; D024841:Fluoroquinolones / Q000032:analysis*; D006041:Goats; D007668:Kidney / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D012756:Sheep; D013552:Swine
D000890:Anti-Infective Agents; D024841:Fluoroquinolones