[Study on the preparation of plasmid DNA-containing liposomes and their properties].
OBJECTIVE
To prepare plasmid DNA-containing liposomes using detergent removal method and investigate the properties of liposomes.


METHODS
Using soybean phospholipid and DDAB as lipid materials and Myrj53 as surface modified material, we prepared liposomes by means of detergent removal technique. The shape, size and zeta potential of the liposomes were characterized by TEM and laser particle size analyzer, respectively. The encapsulation efficiency was also investigated. The ability of liposomes to protect the plasmid DNA from degradation was investigated through incubation with DNase I for different time.


RESULTS
The liposomes were relatively and regularly spherical in shape and had an average size of 72.73 +/- 7.14 (n = 3) nm with the polydispersity index of 0.288+/-0.093 (n = 3). The zeta potential of the liposomes was -23.77+/- 3.51 (n = 3) mV. The encapsulation efficiency of DNA reached 88.13% +/- 4.41% (n = 3). Liposomes could provide DNA with enough protection from degradation by DNase I .


CONCLUSIONS
Detergent removal method is effective and practicable for preparing small-sized plasmid DNA-containing liposomes.
Zhang
Hong-Wei
HW
Key Lab of Drug Targeting, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Sun
Xun
X
Zhang
Zhi-Rong
ZR
D004247:DNA; D003850:Deoxyribonuclease I; D003902:Detergents / Q000737:chemistry; D015316:Genetic Therapy; D005822:Genetic Vectors; D008081:Liposomes / Q000737:chemistry*; D053758:Nanoparticles; D010316:Particle Size; D010957:Plasmids; D014162:Transfection
D003902:Detergents; D008081:Liposomes; D004247:DNA; D003850:Deoxyribonuclease I