2,6-Dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester: a fluorogenic hydrophilic derivatizing reagent for liquid chromatographic analysis of aliphatic amines.
The use of a fluorogenic, hydrophilic, and amine-reactive reagent, 2,6-dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester (DMQC-OSu) has been investigated in the procolumn derivatization for the LC separation of aliphatic amines. In pH 8.0 aqueous medium, DMQC-OSu reacted with amines at 50 degrees C within 20 min to form highly fluorescent carboxamides and the excess reagent hydrolyzed to the corresponding carboxylic acid. The separation of representative amine derivatives with DMQC-OSu has been performed using a C18 column with the fluorescence detection at 326/409 nm. The detection limits reached nanomolar level. The proposed method has been applied to the analysis of real samples with recoveries of 94-108%. Compared with other succinimidyl esters used in the derivatization of amino compounds, DMQC-OSu and its hydrolysate had negligible fluorescence (phi(fl) = 0.09 and 0.02, respectively), which implied that small peaks appeared in chromatograms and slight interference was introduced to the determination.
Wang
Hong
H
Department of Chemistry, Wuhan University, Wuhan, China.
Liu
Xin
X
Zhang
Niu
N
Cao
Li-Wei
LW
Zhang
Hua-Shan
HS
D000588:Amines / Q000032:analysis*; D002264:Carboxylic Acids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D004952:Esters / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008422:Materials Testing; D015394:Molecular Structure; D011804:Quinolines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013388:Succinimides / Q000737:chemistry*
D000588:Amines; D002264:Carboxylic Acids; D004952:Esters; D005456:Fluorescent Dyes; D011804:Quinolines; D013388:Succinimides