Use of semiconductor-based oligonucleotide microarrays for influenza a virus subtype identification and sequencing.
In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here we describe a genome assay and microarray design that can be used to rapidly identify influenza A virus hemagglutinin subtypes 1 through 15 and neuraminidase subtypes 1 through 9. Also described is an array-based enzymatic assay that can be used to sequence portions of both genes or any other sequence of interest.
Lodes
Michael J
MJ
CombiMatrix Corporation, 6500 Harbour Heights Parkway, Suite 301, Mukilteo, WA 98275, USA. mlodes@combimatrix.com
Suciu
Dominic
D
Elliott
Mark
M
Stover
Axel G
AG
Ross
Marty
M
Caraballo
Marcelo
M
Dix
Kim
K
Crye
James
J
Webby
Richard J
RJ
Lyon
Wanda J
WJ
Danley
David L
DL
McShea
Andrew
A
D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D009980:Influenza A virus / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D009439:Neuraminidase / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012666:Semiconductors; D017422:Sequence Analysis, DNA
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase