Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles.
A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A(260)-A(320)):(A(280)-A(320)) ratio of the extracted DNA was 1.9+/-0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods.
Ota
Hiroyuki
H
Malcom Co., Ltd.,4-15-10 Honmachi Shibuya-ku, Tokyo 151-0071, Japan.
Lim
Tae-Kyu
TK
Tanaka
Tsuyoshi
T
Yoshino
Tomoko
T
Harada
Manabu
M
Matsunaga
Tadashi
T
D001331:Automation / Q000295:instrumentation / Q000379:methods*; D001426:Bacterial Proteins / Q000737:chemistry; D018744:DNA, Plant / Q000302:isolation & purification*; D004268:DNA-Binding Proteins / Q000138:chemical synthesis; D018628:Gene Dosage; D018745:Genome, Plant; D016014:Linear Models; D008280:Magnetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012821:Silanes / Q000737:chemistry*; D003313:Zea mays / Q000235:genetics*
D001426:Bacterial Proteins; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D012821:Silanes