title: An inverse correlation of human peripheral blood regulatory T cell frequency with the disease activity of ulcerative colitis.
abstract: Evidence suggests that CD4+CD25+ regulatory T cells play a crucial role in the suppression of intestinal inflammation. However, their role in the suppression of inflammatory bowel disease has not yet been addressed. We examined the proportion of regulatory T cells in inflammatory bowel disease. First, we isolated CD4+CD45RO+CD25+ T cells from the peripheral blood of healthy persons and showed that these cells suppressed T cell proliferation profoundly and expressed FoxP3 abundantly, revealing that they are regulatory cells. Then the proportion of CD45RO+CD25+ in peripheral blood CD4+ T cells was analyzed in patients and healthy controls by flow cytometry. CD4+CD45RO+CD25+ T cell frequency was significantly lower in active ulcerative colitis than in the control and inactive ulcerative colitis. CD4+CD45RO+CD25+ T cell frequency was inversely correlated with the clinical and endoscopic severity of ulcerative colitis. These results suggest that a deficiency of regulatory T cells is associated with the progression of ulcerative colitis.
authors.affiliation: Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Maidashi, Fukuoka, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakamura
authors.forename: Kuniomi
authors.initials: K
authors.lastname: Honda
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Takahiro
authors.initials: T
authors.lastname: Mizutani
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Araki
authors.forename: Teppei
authors.initials: T
authors.lastname: Kabemura
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Chijiiwa
authors.forename: Naohiko
authors.initials: N
authors.lastname: Harada
authors.forename: Hajime
authors.initials: H
authors.lastname: Nawata
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D015415:Biomarkers / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016022:Case-Control Studies; D002478:Cells, Cultured; D003093:Colitis, Ulcerative / Q000097:blood* / Q000503:physiopathology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D017493:Leukocyte Common Antigens / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011336:Probability; D015375:Receptors, Interleukin-2 / Q000276:immunology; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D015415:Biomarkers; D015375:Receptors, Interleukin-2; D017493:Leukocyte Common Antigens