Solution structure of Asl1650, an acyl carrier protein from Anabaena sp. PCC 7120 with a variant phosphopantetheinylation-site sequence.
Cyanobacteria, such as Anabaena, produce a variety of bioactive natural products via polyketide synthases (PKS), nonribosomal peptide synthetases (NRPS), and hybrid peptide/polyketide pathways. The protein Asl1650, which is a member of the acyl carrier protein family from the cyanobacterium Anabaena sp. PCC 7120, is encoded in a region of the Anabaena genome that is rich in PKS and NRPS genes. To gain new insight into the physiological role of acyl carriers in Anabaena, the solution structure of Asl1650 has been solved by NMR spectroscopy. The protein adopts a twisted antiparallel four-helix bundle fold, with a variant phosphopantetheine-attachment motif positioned at the start of the second helix. Structure comparisons with proteins from other organisms suggest a likely physiological function as a discrete peptidyl carrier protein.
Johnson
Margaret A
MA
The Scripps Research Institute (TSRI), Department of Molecular Biology and Joint Center for Structural Genomics (JCSG), La Jolla, California 92037, USA.
Peti
Wolfgang
W
Herrmann
Torsten
T
Wilson
Ian A
IA
Wüthrich
Kurt
K
D000213:Acyl Carrier Protein / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D017033:Anabaena / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010204:Pantetheine / Q000031:analogs & derivatives* / Q000378:metabolism; D010453:Peptide Synthases / Q000737:chemistry; D048630:Polyketide Synthases / Q000737:chemistry; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
D000213:Acyl Carrier Protein; D010204:Pantetheine; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase; C003129:4'-phosphopantetheine