[Research of the feeding strategy in the fermentation of recombinant human fibreblast growth factor mutant].
Human acidic fibreblast growth factor (haFGF) was a kind of cell growth factor with wide bio-activity on cell from mesectoderm and neuro-ectoderm.In this paper, the effect of acetate concentration on the growth and expression of recombinant human acidic fibroblast growth factor mutant system E.coli BL21(DE3)/pET3C-haFGF was investigated. Four fed-batch modes: batch-fed, batch-DO static balance, DO static balance-glucose starvation, and pH-static state were investigated. The accumulation of acetate during the fermentation course was effectively inhibited. The OD600nm value was about 22, after purification, the soluble rhaFGF yielded 450mg/L. During the fermentation, no special ways such as pure oxygen, pressure were adopted, thus the established process would be easily scaled up for industry purpose.
Yuan
Hui
H
Bio-natural Pharmaceutical Institute of Wenzhou Medical College, Wenzhou 325035, China.
Li
Xiao-Kun
XK
Yang
Shu-Lin
SL
D019342:Acetic Acid / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D016220:Fibroblast Growth Factor 1 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D050505:Mutant Proteins / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*
D003470:Culture Media; D050505:Mutant Proteins; D011994:Recombinant Proteins; D016220:Fibroblast Growth Factor 1; D019342:Acetic Acid