A genetic interaction between the vesicular acetylcholine transporter VAChT/UNC-17 and synaptobrevin/SNB-1 in C. elegans.
Acetylcholine, a major excitatory neurotransmitter in Caenorhabditis elegans, is transported into synaptic vesicles by the vesicular acetylcholine transporter encoded by unc-17. The abnormal behavior of unc-17(e245) mutants, which have a glycine-to-arginine substitution in a transmembrane domain, is markedly improved by a mutant synaptobrevin with an isoleucine-to-aspartate substitution in its transmembrane domain. These results suggest an association of vesicular soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) components with vesicular neurotransmitter transporters.
Sandoval
Gisela M
GM
Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02115, USA.
Duerr
Janet S
JS
Hodgkin
Jonathan
J
Rand
James B
JB
Ruvkun
Gary
G
D000818:Animals; D001120:Arginine / Q000235:genetics; D001522:Behavior, Animal; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D005455:Fluorescent Antibody Technique / Q000379:methods; D007532:Isoleucine / Q000235:genetics; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology; D009154:Mutation / Q000502:physiology; D050683:R-SNARE Proteins / Q000235:genetics*; D050494:Vesicular Acetylcholine Transport Proteins / Q000235:genetics*
D029742:Caenorhabditis elegans Proteins; D050683:R-SNARE Proteins; C082365:Unc-17 protein, C elegans; D050494:Vesicular Acetylcholine Transport Proteins; D007532:Isoleucine; D001120:Arginine