Role of the kallikrein-kinin system in Ang-(1-7)-induced vasodilation in mesenteric arterioles of Wistar rats studied in vivo-in situ.
Angiotensin-(1-7) [Ang-(1-7)], exerts a variety of actions in the cardiovascular system, with an important effect being vasodilation. In this work, we investigated the relationship between the vasodilatory activity of Ang-(1-7) and the kallikrein-kinin system. Intravital microscopy was used to study the vasodilation caused by Ang-(1-7) in the mesenteric vascular bed of anesthetized Wistar rats. The topical application of Ang-(1-7) caused vasodilation of mesenteric arterioles that was reduced by A-779, JE 049 and peptidase inhibitors (aprotinin, SBTI, PKSI 527, E-64, PMSF). These results indicated that the vasodilation induced by Ang-(1-7) in the mesenteric arterioles of Wistar rats was heavily dependent on the activation of kallikrein and subsequent kinin formation.
Marangoni
Rossana Anderson
RA
Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes 1524, Cidade Universitária, 05508-900 São Paulo, SP, Brazil.
Carmona
Adriana Karaoglanovic
AK
Passaglia
Rita Cássia A Tostes
RC
Nigro
Dorothy
D
Fortes
Zuleica Bruno
ZB
de Carvalho
Maria Helena Catelli
MH
D000818:Animals; D007611:Aprotinin / Q000737:chemistry / Q000378:metabolism; D001160:Arterioles / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry*; D016234:Kallikrein-Kinin System; D007610:Kallikreins / Q000737:chemistry*; D008297:Male; D010447:Peptide Hydrolases / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012694:Serine / Q000737:chemistry*; D014664:Vasodilation
D012694:Serine; D007611:Aprotinin; D010447:Peptide Hydrolases; D007610:Kallikreins; D003545:Cysteine