title: Comparison of the polymerase chain reaction-restriction fragment length polymorphism pattern of the fiber gene and pathogenicity of serotype-1 fowl adenovirus isolates from gizzard erosions and from feces of clinically healthy chickens in Japan.
abstract: The fiber gene sequence and pathogenicity of the serotype-1 fowl adenovirus (FAdV-1) isolated from gizzard erosions and from clinically normal chickens were compared among isolates. The FAdV-99ZH strain, which induced gizzard erosions, had a nucleotide sequence of the long fiber gene that was different from that of the Ote strain, which did not induce gizzard erosions. The differences could be distinguished by use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The long fiber gene of 16 FAdV-1 isolates from gizzard erosions and 10 FAdV-1 isolates from the feces of clinically normal chickens was examined by use of PCR-RFLP analysis. All 16 FAdV-1 isolates from gizzard erosions had the same restriction patterns as those of strain 99ZH; however, 10 FAdV-1 isolates from normal chickens were classified into 3 groups. Specific-pathogen-free (SPF) chickens were inoculated orally with 2 FAdV-1 isolates from gizzard erosions or 3 FAdV-1 isolates from clinically normal chickens to determine the pathogenicity of each strain. Two of 2 FAdV-1 isolates from gizzard erosions induced gizzard erosions. Two of 3 FAdV-1 isolates from normal chickens had the same PCR-RFLP patterns as those of the Ote strain, but did not induce any gizzard erosions. However, 1 FAdV-1 isolate from clinically normal chickens had the same PCR-RFLP pattern as that of strain 99ZH and induced gizzard erosions. These results indicate that there are FAdV-1 strains that have different pathogenicity; one strain induces gizzard erosions, and the other does not. Use of PCR-RFLP analysis of long fiber genes may be able to distinguish between these two strains.
authors.affiliation: JA Zen-noh (National Federation of Agricultural Co-operative Associations), Institute of Animal Health, Sakura, Chiba, Japan. okudayo@zk.zennoh.or.jp
authors.forename: Yo
authors.initials: Y
authors.lastname: Okuda
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ono
authors.forename: Isao
authors.initials: I
authors.lastname: Shibata
authors.forename: Shizuo
authors.initials: S
authors.lastname: Sato
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
mesh_terms: D000257:Adenoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D002645:Chickens / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000821:virology; D025681:Fowl adenovirus A / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005895:Gizzard, Avian / Q000473:pathology / Q000821:virology*; D007564:Japan; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D011201:Poultry Diseases / Q000473:pathology / Q000821:virology*; D013272:Stomach Diseases / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D014774:Virulence
chemical_list: D004279:DNA, Viral