title: Morphological and molecular characteristics of Echinococcus multilocularis and Echinococcus granulosus mixed infection in a dog from Xinjiang, China.
abstract: The Xinjiang plateau of western China has been shown to have a high prevalence for human cystic echinococcosis (CE) caused by Echinococcus granulosus, and human alveolar echinococcosis (AE) caused by Echinococcus multilocularis. The domestic dog is suspected to be the primary definitive host for the transmission of both E. granulosus and E. multilocularis to humans in this locality. Seventeen of 30 stray dogs from Hejing County of Xinjiang were found positive for E. granulosus post mortem, and one double infection was suspected. Worm samples were collected, dyed by carmine, and observed microscopically. Carmine staining examination clearly revealed the differences in number of proglottids and appearance of uterine branches and lateral genital pore for those two species of Echinococcus. Furthermore, gene target DNA fragments were amplified for formal identification of the two parasite species, based on 12s rRNA mitochondrial gene. The PCR products were purified and sequenced. Compared with NCBI GenBank, the DNA sequences demonstrated 100% identity with E. granulosus (sheep strain, G1 genotype) and E. multilocularis.
authors.affiliation: Xinjiang Hydatid Clinical Research Institute, First Teaching Hospital, Xinjiang Medical University, Urumqi 830054, PR China [corrected]
authors.forename: Yalou
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jean-Mathieu
authors.initials: JM
authors.lastname: Bart
authors.forename: Patrick
authors.initials: P
authors.lastname: Giraudoux
authors.forename: Philip
authors.initials: P
authors.lastname: Craig
authors.forename: Dominique
authors.initials: D
authors.lastname: Vuitton
authors.forename: Hao
authors.initials: H
authors.lastname: Wen
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D018509:DNA, Helminth / Q000032:analysis*; D004197:Disease Reservoirs / Q000662:veterinary; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D004443:Echinococcosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D048210:Echinococcus multilocularis / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012335:RNA, Ribosomal / Q000032:analysis*; D016415:Sequence Alignment
chemical_list: D018509:DNA, Helminth; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S