title: Two new Fusicoccum species from Acacia and Eucalyptus in Venezuela, based on morphology and DNA sequence data.
abstract: Botryosphaeria spp. are common endophytes of woody plants, and they also include some serious pathogens of Eucalyptus and Acacia species. Numerous anamorphs have been associated with Botryosphaeria, of which the species Fusicoccum are amongst the most common. Here, we characterize two new Fusicoccum species, isolated from Eucalyptus and Acacia trees in Venezuela, based on morphological features in culture and comparisons of DNA sequence data. The two taxa named Fusicoccum andinum and F. stromaticum spp. nov, reside in two well-supported clades (BS values = 100%) based on a combined data set of the ITS of the rDNA operon and translation elongation factor 1-alpha (EF1- alpha) gene sequences. The conidia of F. andinum are unusually large amongst Botryosphaeria anamorphs, and peripherally resemble those of B. mamane and B. melanops. F. stromaticum is characterized by large conidiomata in cultures, growth at 35 degrees C and slightly thickened conidial walls, which is different to most other Fusicoccum spp. No teleomorphs were observed for these fungi, but DNA sequence data show that they are anamorphs of Botryosphaeria.
authors.affiliation: Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, 74 Lunnon Road, Hillcrest, Pretoria 0002, South Africa. msari@ula.ve
authors.forename: Sari
authors.initials: S
authors.lastname: Mohali
authors.forename: Bernard
authors.initials: B
authors.lastname: Slippers
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Wingfield
mesh_terms: D000045:Acacia / Q000382:microbiology*; D001203:Ascomycota / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D001483:Base Sequence; D002965:Classification; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005052:Eucalyptus / Q000382:microbiology*; D020648:Peptide Elongation Factor 1 / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014687:Venezuela
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D020648:Peptide Elongation Factor 1