[Preparation and purification of polyclonal antibody against chymopapain].
OBJECTIVE
To prepare and purify polyclonal antibody against chymopapain, and to make a foundation for establishing an immunossay for chymopapain.


METHODS
New Zealand rabbit was immunized with chymopapain. Antiserum was purified by Protein A and analyzed by ELISA.


RESULTS
The titer of the antiserum obtained in this experiment by ELISA was up to 1:380000 and the purity was proved to be high by SDS-PAGE.
Ding
Yu-lan
YL
Guangzhou General Hospital of Guangzhou Military Area, Guangzhou 510010, China.
Yang
Tai-cheng
TC
Zhao
Shu-jin
SJ
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D031042:Asimina / Q000737:chemistry*; D002917:Chymopapain / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D010206:Papain / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits
D000906:Antibodies; D010206:Papain; D002917:Chymopapain