title: [A comparative study on the resistance of bacteriophage T4, phiXM174D, and f2 to glutaraldehyde in laboratory].
abstract: OBJECTIVE
  To identify the best indicative bacteriophage in disinfection tests through comparing the resistance of bacteriophage T4, phiX174D, and f2 to glutaraldehyde.
  
  
  METHODS
  The virucidal activities of glutaraldehyde against bacteriophage T4, phiX174D, and f2 were assessed with suspension tests along with neutralizer tests. The double-agar-layer plaque technique was used to detect the bacteriophage T4, phiX174D, and f2.
  
  
  RESULTS
  (1)In a condition of 3000 mg/L of glutaraldehyde and 20 minutes of contact or 6000 mg/L of glutaraldehyde and 5 minutes of contact, "disinfection" level for bacteriophage T4 was achieved, with the log10 inactivation value (LIV) or log10o reduction value (LRV) (=log10No-log10Nt) > or = 4. 00 log10. (2) In a condition of 2500 mg/L of glutaraldehyde and 20 minutes of contact or 5000 mg/L of glutaraldehyde and 5 minutes of contact, the LIV for bacteriophage phiX174D reached "disinfection" level; (3) In a condition of 4000 mg/L of glutaraldehyde and 40 minutes of contact or 8000 mg/L of glutaraldehyde and 10 minutes of contact, the LIV for bacteriophage f2 reached "disinfection" level.
  
  
  CONCLUSIONS
  Bacteriophage f2 and bacteriophage phiX174D has the strongest and weakest resistance to glutaraldehyde respectively.
authors.affiliation: Department of Medical Laboratory Technology, West China School of Public health, Sichuan University, Chengdu 610041, China.
authors.forename: Zhao-Bin
authors.initials: ZB
authors.lastname: Chen
authors.forename: Chao-Wu
authors.initials: CW
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Wang
mesh_terms: D017122:Bacteriophage T4 / Q000187:drug effects*; D010584:Bacteriophage phi X 174 / Q000187:drug effects*; D001435:Bacteriophages / Q000187:drug effects; D004203:Disinfection; D024882:Drug Resistance, Viral; D005976:Glutaral / Q000494:pharmacology*
chemical_list: D005976:Glutaral