title: Intraretinal and periretinal pathology in anterior proliferative vitreoretinopathy.
abstract: OBJECTIVE
  To determine the intraretinal and periretinal pathological changes in early anterior proliferative vitreoretinopathy (APVR).
  
  
  METHODS
  Observational case series.
  
  
  METHODS
  Eighteen patients undergoing retinectomy for APVR.
  
  
  METHODS
  Retinectomy specimens removed at vitrectomy surgery were analysed by (a) semithin light microscopy, (b) immunohistochemistry and (c) electron microscopy.
  
  
  RESULTS
  The specimens showed consistent outer retinal degenerative changes, marked Muller cell hypertrophy and glial continuity to epiretinal membranes. Photoreceptor outer and inner segments were markedly disrupted and occasional photoreceptor nuclear had pyknosis and chromatin clumping consistent with apoptosis. Muller cells expressed upregulated levels of glial fibrillary acid protein (GFAP) and extended through glial bridges to complex epiretinal membranes which in some areas had a bilaminar structure with a glial-negative inner lamina.
  
  
  CONCLUSIONS
  Retinal degeneration and photoreceptor apoptosis occur in retinal detachment complicated by proliferative vitreoretinopathy (PVR), although during the early stages of the process neural retinal cells remain present, suggesting potential for recovery. The intraretinal glial response appears to be centrally involved in the formation of contractile epiretinal membranes. The retina retains the capacity for a degree of functional recovery following surgery for PVR. Surgical separation of anterior epiretinal membranes in PVR may be difficult and incomplete and alternative surgical strategies may be necessary to prevent recurrence.
authors.affiliation: Moorfields Eye Hospital, City Road, London, EC1V 2PD, UK. david.charteris@moorfields.nhs.uk
authors.forename: David G
authors.initials: DG
authors.lastname: Charteris
authors.forename: John
authors.initials: J
authors.lastname: Downie
authors.forename: G William
authors.initials: GW
authors.lastname: Aylward
authors.forename: Charanjit
authors.initials: C
authors.lastname: Sethi
authors.forename: Philip
authors.initials: P
authors.lastname: Luthert
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D019773:Epiretinal Membrane / Q000209:etiology / Q000473:pathology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D009457:Neuroglia / Q000378:metabolism / Q000648:ultrastructure; D013508:Ophthalmologic Surgical Procedures; D020419:Photoreceptor Cells, Vertebrate / Q000648:ultrastructure; D012160:Retina / Q000473:pathology* / Q000601:surgery; D012162:Retinal Degeneration / Q000209:etiology / Q000473:pathology; D012163:Retinal Detachment / Q000209:etiology / Q000473:pathology; D015854:Up-Regulation; D018630:Vitreoretinopathy, Proliferative / Q000150:complications / Q000473:pathology* / Q000601:surgery
chemical_list: D005904:Glial Fibrillary Acidic Protein