Permeability of ovine primordial follicles to different cryoprotectants.
OBJECTIVE
To determine the behavior of isolated primordial follicles that were exposed to different concentrations of dimethyl sulfoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH), and glycerol (GLY).


METHODS
Isolated primordial follicles were exposed to the cryoprotectant (CPA) solution and photographed to calculate their volume at different periods of exposure.


METHODS
Laboratorio Renzo Giuliani, University of Florence, Italy.


METHODS
Lambs, 30-40 days old.


METHODS
Isolation of primordial follicles and subsequent exposure to CPA.


METHODS
Follicular volume.


RESULTS
At 2 minutes of CPA exposure, all follicles appeared to be shrunken. At approximately 5 minutes, shrinkage ceased, and follicles started to swell, absorbing the CPA and water to maintain osmotic equilibrium. When DMSO was tested, follicular dehydration in all concentrations did not exceed 17%; with PROH and EG, it reached 33% and 27%, respectively. The highest degree of dehydration (48%) was seen with GLY. In almost all tested concentrations, follicular shrinkage occurred up to 5 minutes.


CONCLUSIONS
Volume changes in isolated primordial follicles can fluctuate according to the CPA used and its concentration.
Amorim
Christiani Andrade
CA
Hospital Veterinário Prédio 97, University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil. amorimchris@yahoo.com
Rondina
Davide
D
Lucci
Carolina Madeira
CM
Gonçalves
Paulo Bayard Dias
PB
Figueiredo
José Ricardo de
JR
Giorgetti
Alessandro
A
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D003451:Cryoprotective Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D019610:Cytoprotection / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D019188:Organ Preservation Solutions / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006080:Ovarian Follicle / Q000502:physiology*; D010539:Permeability / Q000187:drug effects; D012756:Sheep
D003451:Cryoprotective Agents; D019188:Organ Preservation Solutions