title: Coengagement of CD16 and CD94 receptors mediates secretion of chemokines and induces apoptotic death of naive natural killer cells.
abstract: Down-modulation of CD16 (FcgammaRIII) receptors and loss of natural killer (NK) cell function have been observed in oral cancer patients. However, neither the mechanisms nor the significance of the decrease in CD16 receptors have been fully understood. The cytotoxic activity and survival of NK cells are negatively regulated by antibodies directed against CD16 surface receptor. The addition of anti-CD94 antibody in combination with either F(ab')(2) fragment or intact anti-CD16 antibody to NK cells resulted in significant inhibition of NK cell cytotoxic function and induction of apoptosis in resting human peripheral blood NK cells. Addition of interleukin-2 to anti-CD16 and/or anti-CD94 antibody-treated NK cells significantly inhibited apoptosis and increased the function of NK cells. There was a significant increase in tumor necrosis factor-alpha (TNF-alpha) but not IFN-gamma secretion in NK cells treated either with anti-CD16 antibody alone or in combination with anti-CD94 antibodies. Consequently, the addition of anti-TNF-alpha antibody partially inhibited apoptosis of NK cells mediated by the combination of anti-CD94 and anti-CD16 antibodies. Increase in apoptotic death of NK cells also correlated with an increase in type 2 inflammatory cytokines and in the induction of chemokines. Thus, we conclude that binding of antibodies to CD16 and CD94 NK cell receptors induces death of the NK cells and signals for the release of chemokines.
authors.affiliation: The Jane and Jerry Weintraub Center for Reconstructive Biotechnology and Division of Oral Biology and Medicine, Jonsson Comprehensive Cancer Center, University of California at Los Angeles, Los Angeles, California, USA. ajewett@ucla.edu
authors.forename: Anahid
authors.initials: A
authors.lastname: Jewett
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Cacalano
authors.forename: Christian
authors.initials: C
authors.lastname: Head
authors.forename: Antonia
authors.initials: A
authors.lastname: Teruel
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000187:drug effects / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D018925:Chemokines / Q000378:metabolism*; D058851:GPI-Linked Proteins; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects / Q000502:physiology*; D051920:NK Cell Lectin-Like Receptor Subfamily D / Q000187:drug effects / Q000378:metabolism*; D017452:Receptors, IgG / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018925:Chemokines; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D015850:Interleukin-6; C497869:KLRD1 protein, human; D051920:NK Cell Lectin-Like Receptor Subfamily D; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha