title: Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216.
abstract: We isolated two lipase genes LIPY7, LIPY8 from Yarrowia lipolytica CGMCC (China general microbiological culture collection center) AS 2.1216. The LIPY7 and LIPY8 genes encode a 366 and a 371-amino acid protein, respectively. The lipase genes with 6 x His tag sequence were cloned into expression vector pPIC9K and successfully integrated into a heterologous fungal host Pichia pastoris KM71, respectively. The recombinants were induced by methanol to secrete active lipases into cultural medium. The recombinant lipases were also purified and characterized.
authors.affiliation: College of Chemistry and Material Science, Hubei University, Wuhan, PR China.
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Song
authors.forename: Zheng-Bing
authors.initials: ZB
authors.lastname: Jiang
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Ma
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D003001:Cloning, Molecular; D015870:Gene Expression; D008049:Lipase / Q000096:biosynthesis / Q000302:isolation & purification*; D010843:Pichia; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D025062:Yarrowia / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D008049:Lipase