A human-horse comparative map based on equine BAC end sequences.
In an effort to increase the density of sequence-based markers for the horse genome we generated 9473 BAC end sequences (BESs) from the CHORI-241 BAC library with an average read length of 677 bp. BLASTN searches with the BESs revealed 4036 meaningful hits (E <or= 10(-5)) in the human genome that provide useful markers for the human-horse comparative map. The 4036 BLASTN hits allowed the anchoring of 3079 BAC clones to the human genome, on average one corresponding equine BAC clone per megabase of human DNA. We used the BLASTN anchored BESs for an in silico prediction of the gene content and chromosome assignment of comparatively mapped equine BAC clones. As a first verification of our in silico mapping strategy we placed 19 equine BESs with matches to HSA6 onto the RH map. All markers were assigned to the predicted localizations on ECA10, ECA20, and ECA31, respectively.
Leeb
Tosso
T
Institute of Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Bünteweg 17p, 30559 Hannover, Germany. Tosso.Leeb@itz.unibe.ch
Vogl
Claus
C
Zhu
Baoli
B
de Jong
Pieter J
PJ
Binns
Matthew M
MM
Chowdhary
Bhanu P
BP
Scharfe
Maren
M
Jarek
Michael
M
Nordsiek
Gabriele
G
Schrader
Frank
F
Blöcker
Helmut
H
D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D015723:Gene Library; D005819:Genetic Markers; D015894:Genome, Human; D006736:Horses / Q000235:genetics*; D006801:Humans; D021101:Radiation Hybrid Mapping / Q000379:methods*; D013045:Species Specificity
D017931:DNA Primers; D005819:Genetic Markers