title: Fine tuning of beta-glucosidase inhibitory activity in the 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) system.
abstract: Based on our extensive studies of D-glucosidase inhibiting 2,5-dideoxy-2,5-imino-D-mannitol derivatives, we have been trying to create a series of fluorescent derivatives with a view to an 'inhibitory activity ruler' based on competitive displacement reactions of non-fluorescent inhibitors by fluorescent ones and vice versa, which can be performed and followed in microtiter plates or on-chips. Thus, a set of compounds was assembled with Ki values between 2 nM and 1 microM against Agrobacterium sp. beta-glucosidase.
authors.affiliation: Glycogroup, Institut für Organische Chemie der Technischen Universität Graz, Stremayrgasse 16, A-8010 Graz, Austria. t.wrodnigg@tu-graz.ac.at
authors.forename: Tanja M
authors.initials: TM
authors.lastname: Wrodnigg
authors.forename: Arnold E
authors.initials: AE
authors.lastname: Stütz
authors.forename: Chris A
authors.initials: CA
authors.lastname: Tarling
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Withers
mesh_terms: D050112:Imino Pyranoses / Q000494:pharmacology; D007700:Kinetics; D008353:Mannitol / Q000031:analogs & derivatives* / Q000494:pharmacology; D012231:Rhizobium / Q000201:enzymology; D013329:Structure-Activity Relationship; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors*
chemical_list: C494911:2,5-dideoxy-2,5-imino-D-mannitol; D050112:Imino Pyranoses; D008353:Mannitol; D001617:beta-Glucosidase