title: Solution structure of the antifreeze-like domain of human sialic acid synthase.
abstract: The structure of the C-terminal antifreeze-like (AFL) domain of human sialic acid synthase was determined by NMR spectroscopy. The structure comprises one alpha- and two single-turn 3(10)-helices and two beta-strands, and is similar to those of the type III antifreeze proteins. Evolutionary trace analyses of the type III antifreeze protein family suggested that the class-specific residues in the human and bacterial AFL domains are important for their substrate binding, while the class-specific residues of the fish antifreeze proteins are gathered on the ice-binding surface.
authors.affiliation: RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hamada
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ito
authors.forename: Takamasa
authors.initials: T
authors.lastname: Abe
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Hayashi
authors.forename: Peter
authors.initials: P
authors.lastname: Güntert
authors.forename: Makoto
authors.initials: M
authors.lastname: Inoue
authors.forename: Takanori
authors.initials: T
authors.lastname: Kigawa
authors.forename: Takaho
authors.initials: T
authors.lastname: Terada
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yoshida
authors.forename: Akiko
authors.initials: A
authors.lastname: Tanaka
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hirota
mesh_terms: D000595:Amino Acid Sequence; D021343:Antifreeze Proteins, Type III / Q000737:chemistry*; D019143:Evolution, Molecular; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D007652:Oxo-Acid-Lyases / Q000737:chemistry*; D010802:Phylogeny; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D012996:Solutions; D013329:Structure-Activity Relationship
chemical_list: D021343:Antifreeze Proteins, Type III; D012996:Solutions; C018730:N-acetylneuraminate synthase; D007652:Oxo-Acid-Lyases