title: Preliminary study of simultaneous multi-anticoagulant deficiency diagnosis by fiber optic multi-analyte biosensor.
abstract: Protein C (PC), protein S (PS), antithrombin III, and plasminogen are four important anticoagulants in blood plasma. Deficiency of any of these biomolecules may lead to thrombo-embolic complications including lung embolism, heart attack, and stroke. A multi-factor sensing system is beneficial for identifying the cause of abnormal blood clotting more effectively, rapidly, and cost-effectively. As an initial effort toward simultaneous multi-anticoagulant detection, a PC and PS dual-sensing system has been under development in our research group. A fiberoptic PC biosensor utilizing fluorophore-mediated sandwich immunoassay was already developed for rapid (-5 minutes) PC deficiency diagnosis. After a single PS sensor was developed for the PS deficiency diagnosis, the two sensors were connected in series to form a dual-sensing system. The cross-reactivity between the analytes and the sensors was found to be minimal. For easier sensing operation, a mixture of fluorophore-linked anti-PC and anti-PS was applied. The results showed that the mixture can be used with a slight signal reduction. When PC and PS was mixed in a sample, the signal intensity was decreased by approximately 5% for both sensors. A study is currently being performed to overcome the signal reduction by increasing the flow velocity and incubation time.
authors.forename: Liang
authors.initials: L
authors.lastname: Tang
authors.forename: Kyung A
authors.initials: KA
authors.lastname: Kang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003429:Cross Reactions; D005336:Fiber Optic Technology / Q000295:instrumentation; D006801:Humans; D051379:Mice; D055100:Optical Fibers; D011486:Protein C / Q000032:analysis* / Q000276:immunology; D020151:Protein C Deficiency / Q000097:blood / Q000175:diagnosis*; D017293:Protein S / Q000032:analysis* / Q000276:immunology; D018455:Protein S Deficiency / Q000097:blood / Q000175:diagnosis*
chemical_list: D000911:Antibodies, Monoclonal; D011486:Protein C; D017293:Protein S