Nuclear DNA content of Vitis vinifera cultivars and ploidy level analyses of somatic embryo-derived plants obtained from anther culture.
Flow cytometry was employed to determine the ploidy level of Vitis vinifera L. somatic embryo-derived plants obtained from anther culture. Only one among the 41 analysed plants (2.4%) presented somaclonal variation (tetraploidy); the other plants were diploid. No significant differences (P<or=0.05) were detected between diploid and parental field plants. No haploid or aneuploid plants were observed. The nuclear DNA content of nine V. vinifera cultivars was also estimated using flow cytometry. A non-significant variation was found among the cultivars, with DNA content ranging from 1.17 pg/2C (cv. 'Tinta Barroca' and 'Viosinho') to 1.26 pg/2C (cv. 'Cabernet Sauvignon'). These results and previous studies on other Vitis species suggest that Vitis genome is stable with regard to nuclear DNA content.
Leal
F
F
Center of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
Loureiro
J
J
Rodriguez
E
E
Pais
M S
MS
Santos
C
C
Pinto-Carnide
O
O
D002467:Cell Nucleus / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D035264:Flowers / Q000254:growth & development*; D018515:Plant Leaves; D011003:Ploidies; D012639:Seeds; D046509:Tissue Culture Techniques; D027843:Vitis / Q000166:cytology / Q000196:embryology* / Q000235:genetics*
D018744:DNA, Plant