Modification of a mammalian cell protein in the presence of [32P-adenylate]NAD: evidence for ADP ribosylation activity associated with Helicobacter pylori.
Culture filtrates from Helicobacter pylori promote the transfer of the radiolabel from [32P-adenylate]NAD to one or more heat-labile factors within extracts prepared from several mammalian cell lines, with the predominate radiolabeled species exhibiting an apparent molecular mass of greater than 130 kDa. Our results suggest that several H. pylori strains release a factor that ADP-ribosylates a mammalian target protein.
Nossa
Carlos W
CW
Department of Microbiology, University of Illinois, 601 South Goodwin Ave., Urbana, IL 61801, USA.
Blanke
Steven R
SR
D036002:ADP Ribose Transferases / Q000378:metabolism*; D006367:HeLa Cells; D016480:Helicobacter pylori / Q000201:enzymology*; D006801:Humans; D009243:NAD / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D011506:Proteins / Q000378:metabolism*
D010761:Phosphorus Radioisotopes; D011506:Proteins; D009243:NAD; D036002:ADP Ribose Transferases