Determination of scutellarin in mouse plasma and different tissues by high-performance liquid chromatography.
A simple HPLC-UV method was developed for the determination of scutellarin in plasma and different tissues of mice (heart, liver, spleen, lungs and kidneys). The separation was achieved by HPLC on a Hypersil C(18) column with a mobile phase composed of methanol-water-glacial acetic acid (40:60:1). UV detection was used at 335 nm. The calibration curves were linear in all matrices (r(2)>0.997) in the concentration range of 0.1-10 microg/ml for plasma and 0.1-20 microg/g for tissue homogenates, respectively. The method described is suitable for studies on the distribution of scutellarin in different tissues of mice.
Xiong
Fei
F
Pharmaceutical Research Institute, China Pharmaceutical University, Nanjing 210009, PR China.
Wang
Hao
H
Cheng
Ji
J
Zhu
Jiabi
J
D000818:Animals; D047310:Apigenin / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005965:Glucuronates / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D051379:Mice; D009206:Myocardium / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013154:Spleen / Q000737:chemistry
D005965:Glucuronates; C484876:scutellarin; D047310:Apigenin