title: Nucleophosmin is selectively deposited on mRNA during polyadenylation.
abstract: Nucleophosmin (NPM), an abundant, predominantly nucleolar protein that influences numerous cellular processes, was shown to specifically associate with the bodies of messenger RNAs as a result of the process of 3'-end formation. NPM deposition requires polyadenylation but not the 3' cleavage event to occur on the transcript. Furthermore, the protein does not associate with RNAs bearing a preformed poly(A) tail or with mRNAs that have undergone cleavage but not polyadenylation. A region within 10 bases upstream of the AAUAAA element is required for NPM association, but deposition of the protein seems to be sequence independent. NPM association with poly(A)(+) mRNAs was also demonstrated in vivo. NPM, therefore, represents a mark left on transcripts as a result of 3'-end processing and may have a role in one or more of a variety of post-transcriptional processes influenced by the polyadenylation event.
authors.affiliation: Department of Microbiology, Immunology & Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, Colorado 80523, USA.
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Palaniswamy
authors.forename: Karen C M
authors.initials: KC
authors.lastname: Moraes
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Wilusz
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Wilusz
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D026723:Polyadenylation; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D013379:Substrate Specificity
chemical_list: C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D000090243:Nucleophosmin