title: G-specific RNA-cleaving conjugates of short peptides and oligodeoxyribonucleotides.
abstract: Artificial ribonucleases, conjugates of short oligodeoxyribonucleotides and peptides built of arginine, leucine, proline, and serine, were synthesized and assessed in terms of ribonuclease activity and specificity of RNA cleavage. A specific group of the conjugates was identified that display T1-ribonuclease-like activity and cleave RNA predominantly at G-X sequences. Circular dichroism study of the structures of the most active conjugates, free peptide (LR)4G, and oligonucleotides revealed that conjugation of oligonucleotide to the peptide results in a specific peptide folding that possibly provides ribonuclease activity to the conjugate.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Russian Federation, Novosibirsk, 630090.
authors.forename: Nadezhda L
authors.initials: NL
authors.lastname: Mironova
authors.forename: Dmitrii V
authors.initials: DV
authors.lastname: Pyshnyi
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Stadler
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Prokudin
authors.forename: Yuri I
authors.initials: YI
authors.lastname: Boutorine
authors.forename: Eugenia M
authors.initials: EM
authors.lastname: Ivanova
authors.forename: Marina A
authors.initials: MA
authors.lastname: Zenkova
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Gross
authors.forename: Valentin V
authors.initials: VV
authors.lastname: Vlassov
mesh_terms: D020029:Base Pairing; D002942:Circular Dichroism; D006147:Guanine / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D009838:Oligodeoxyribonucleotides; D009842:Oligopeptides; D006147:Guanine; D012313:RNA; D012260:Ribonucleases