title: Effect of solid storage on caprine semen conservation at 5 degrees C.
abstract: In this work, we investigated the effect of storage in solid-phase extender on buck semen conserved at 5 degrees C. Furthermore, we studied the effect of addition of cysteine to the extender and the washing of seminal plasma on sperm survival. In Experiment 1, milk-based extender (M) was used as a control to study the effect of solid media storage (G) and cysteine supplementation (C), and the combination of both (GC), on in vitro sperm survival of washed and non-washed semen, conserved up to 72 h at 5 degrees C. Motility, acrosome integrity (NAR) and hypo-osmotic swelling tests (HOST) were evaluated to assess in vitro sperm survival. In Experiment 2, an artificial insemination (AI) field trial was performed to compare G versus M. Solid media (G) maintained motility of spermatozoa during storage higher than any other extender (67% G versus 62% GC; 61% M and 59% C; P<0.05), but there was no difference in NAR or HOST between extenders (P>0.05). No improvement in sperm viability was obtained by addition of cysteine to the media. Washing of semen improved motility (65% versus 60%; P<0.05), NAR (70% versus 64%; P<0.05) and HOST (37% versus 28%; P<0.05). No significant differences in fertility were obtained between G and M extenders (47% versus 41%; P>0.05). In conclusion, washing of semen and dilution in gelatin-supplemented milk extender (solid storage) appears to be a successful method for goat semen storage at 5 degrees C.
authors.affiliation: Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Moncada, Valencia, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Salvador
authors.forename: J
authors.initials: J
authors.lastname: Yániz
authors.forename: M P
authors.initials: MP
authors.lastname: Viudes-de-Castro
authors.forename: E A
authors.initials: EA
authors.lastname: Gómez
authors.forename: M A
authors.initials: MA
authors.lastname: Silvestre
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects / Q000502:physiology; D000465:Algorithms; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003080:Cold Temperature; D003451:Cryoprotective Agents / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D005260:Female; D005298:Fertility / Q000187:drug effects / Q000502:physiology; D006041:Goats; D008297:Male; D008954:Models, Biological; D011247:Pregnancy; D018873:Pregnancy Rate; D012661:Semen / Q000187:drug effects; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents; D003545:Cysteine