title: Relationship between DNA ploidy and proliferative cell nuclear antigen index in canine hemangiopericytoma.
abstract: The mitotic index is reported to be correlated with recurrence, mean patient survival, and metastasis of canine hemangiopericytoma (CHP). However, to the authors' knowledge, studies investigating the parameters that can predict recurrence or metastasis of CHP with low mitotic index have not been done. To evaluate growth kinetics of CHP with low mitotic index, a retrospective analysis of the proliferative activity by antiproliferative cell nuclear antigen monoclonal antibody and DNA contents by flow cytometry (FCM) was performed with 21 formalin-fixed and paraffin-embedded CHP samples. Of the 21 tumors evaluated by FCM, 6 (26.6%) were aneuploid tumors, and 15 (71.4%) were diploid tumors. There was significant correlation between the PCNA index and ploidy pattern. The diploid group had 39.1 +/- 9.2 PCNA index, whereas the aneuploid group's proliferative cell nuclear antigen (PCNA) index was 63.1 +/- 8.2. The diploid group had mean mitotic index value of 1.140 +/- 0.855, and the aneuploid group had a mean value of 1.067 +/- 0.767. From these results, the CHP samples with low mitotic index were classified into either the aneuploid group with higher PCNA index or the diploid group with lower PCNA index, suggesting that DNA ploidy and proliferative activity may give an indication about malignancy of CHPs with a low mitotic index.
authors.affiliation: Veterinary Medical Research Center, College of Veterinary Medicine, Chonnam National University, Gwangju, South Korea.
authors.forename: Seong-Kwi
authors.initials: SK
authors.lastname: Kang
authors.forename: Nam-Yong
authors.initials: NY
authors.lastname: Park
authors.forename: Ho-Sung
authors.initials: HS
authors.lastname: Cho
authors.forename: Sung-Shik
authors.initials: SS
authors.lastname: Shin
authors.forename: Mun-Il
authors.initials: MI
authors.lastname: Kang
authors.forename: Sang-Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: Changbaig
authors.initials: C
authors.lastname: Hyun
authors.forename: In-Chul
authors.initials: IC
authors.lastname: Park
authors.forename: Jong-Tack
authors.initials: JT
authors.lastname: Kim
authors.forename: Cheol
authors.initials: C
authors.lastname: Jeong
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Jeong
authors.forename: You-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Kenji
authors.initials: K
authors.lastname: Ochiai
authors.forename: Takashi
authors.initials: T
authors.lastname: Umemura
authors.forename: Kyoung-Oh
authors.initials: KO
authors.lastname: Cho
mesh_terms: D000818:Animals; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D005434:Flow Cytometry / Q000662:veterinary; D006393:Hemangiopericytoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D006651:Histocytochemistry / Q000662:veterinary; D011003:Ploidies; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D012189:Retrospective Studies
chemical_list: D004273:DNA, Neoplasm; D018809:Proliferating Cell Nuclear Antigen