title: Oral delivery of replication-competent adenovirus vectors is well tolerated by SIV- and SHIV-infected rhesus macaques.
abstract: Although replication-competent adenovirus (Ad) vectors are promising in AIDS vaccine design, their safety in immune compromised hosts is unknown. To initially address this question, enteric-coated tablets containing a replicating Ad vector were orally administered to SHIV- and SIV-infected rhesus macaques with normal, intermediate or low CD4 T cell counts and stable disease. The vector was detected within a week after tablet administration in stools of all animals but not in nasal secretions, indicating no spread of virus to the upper respiratory tract. CD4 T cell counts and viral loads remained stable in all animals and no signs of fever, weight loss, or other clinical symptoms of Ad-induced disease were observed during 10 weeks of follow-up. Oral delivery of the replicating Ad vector was safe and well tolerated by SHIV- and SIV-infected hosts. Oral enteric-coated tablets may prove safe for administering replicating Ad-vectored vaccines in areas with high HIV prevalence.
authors.affiliation: NIH, NCI, Vaccine Branch, Bethesda, MD 20892, USA.
authors.forename: V Raúl
authors.initials: VR
authors.lastname: Gómez-Román
authors.forename: George J
authors.initials: GJ
authors.lastname: Grimes
authors.forename: Gopal K
authors.initials: GK
authors.lastname: Potti
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.forename: Thorsten
authors.initials: T
authors.lastname: Demberg
authors.forename: Luisa
authors.initials: L
authors.lastname: Gravlin
authors.forename: James
authors.initials: J
authors.lastname: Treece
authors.forename: Ranajit
authors.initials: R
authors.lastname: Pal
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Lee
authors.forename: W Gregory
authors.initials: WG
authors.lastname: Alvord
authors.forename: Phillip D
authors.initials: PD
authors.lastname: Markham
authors.forename: Marjorie
authors.initials: M
authors.lastname: Robert-Guroff
mesh_terms: D016915:AIDS Vaccines / Q000009:adverse effects* / Q000235:genetics / Q000378:metabolism; D000260:Adenoviruses, Human / Q000235:genetics* / Q000502:physiology*; D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001831:Body Temperature; D018791:CD4 Lymphocyte Count; D005822:Genetic Vectors / Q000235:genetics / Q000502:physiology; D008253:Macaca mulatta / Q000502:physiology* / Q000821:virology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000821:virology*; D014766:Viremia; D014779:Virus Replication
chemical_list: D016915:AIDS Vaccines; D000914:Antibodies, Viral