The effect of asbestos and stone-wool fibres on some chemokines and redox system of pulmonary alveolar macrophages and pneumocytes type II.
The in vitro effect of stone-wool has been studied in primary cultures of pulmonary alveolar macrophages (AM) and type II pneumocytes (T2) by morphological, biochemical and immunological methods. UICC crocidolite was applied as a positive control. Although stone-wool brought about frustrated phagocytosis, it did not induce serious membrane damage, whereas crocidolite gave rise to very severe membrane alterations. Stone-wool significantly reduced the activity of Cu,Zn/superoxide dismutase (SOD) in alveolar macrophages and significantly decreased the activity of gamma-glutamyl transpeptidase (GGT) in pneumocytes type II. Crocidolite, on the other hand, decreased the activity of all enzymes (glutathione peroxidase - GSH-Px, glutathione reductase - GSH-Rd) of glutathione metabolism in alveolar macrophages. It decreased the activity of all enzymes in pneumocytes type II except for Cu,Zn/SOD. After exposure to stone-wool, the production of inflammatory proteins, macrophage chemoattractant protein-1 (MCP-1) and macrophage inhibitory protein-1alpha (MIP-1alpha) increased in both cultured cells but did not reach the level induced by crocidolite. Although this study provided a useful insight in the toxicity of the stone-wool, we can not draw the conclusions how the intact pulmonary tissue may respond on the exposure to these fibres, exclusively based on the in vitro tests.
Tátrai
Erzsébet
E
Fodor József National Center for Public Health, Budapest, Hungary. tatrai@fjokk.hu
Brózik
Marta
M
Kováciková
Zuzana
Z
Horváth
Magdolna
M
D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D017638:Asbestos, Crocidolite / Q000633:toxicity; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism*; D002630:Chemotactic Factors / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D055436:Growth Differentiation Factor 15; D016676:Macrophages, Alveolar / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D019016:Mineral Fibers / Q000633:toxicity*; D010084:Oxidation-Reduction; D011650:Pulmonary Alveoli / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism*
D018925:Chemokines; D002630:Chemotactic Factors; D016207:Cytokines; C526142:Gdf15 protein, rat; D055436:Growth Differentiation Factor 15; D019016:Mineral Fibers; D017638:Asbestos, Crocidolite; D001194:Asbestos; D013482:Superoxide Dismutase