Enhancement of transcriptional activity of DNA complexes by amphoteric PEG derivative.
A water-soluble PEG derivative having both amino and carboxylic acid side chains (PEG-AC) was synthesized and explored for its transcription- and transfection-enhancing activity. PEG-AC could be deposited onto the surface of DNA/polycation complexes to form a ternary complex with slightly negative surface potential. PEG-AC-coating on the plasmid/PEI complexes obviously enhanced their transcriptional activity, and 31-fold higher consumption of UTP was observed. Amphoteric PEG-AC would loosen the tightly compacted DNA/PEI complex and facilitate the approach of transcriptional factors. PEG-AC also evidently improved the transgene expression level on the cultured CHO cells.
Koyama
Yoshiyuki
Y
Department of Textile Science and Department of Food Science, Otsuma Women's University, Tokyo, 102-8357 Japan. koyama@otsuma.ac.jp
Yamashita
Misa
M
Iida-Tanaka
Naoko
N
Ito
Tomoko
T
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D016466:CHO Cells; D002264:Carboxylic Acids / Q000737:chemistry; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008156:Luciferases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011094:Polyethyleneimine / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
D000596:Amino Acids; D002264:Carboxylic Acids; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D004247:DNA; D008156:Luciferases