title: Insights into human CD34+ hematopoietic stem/progenitor cells through a systematically proteomic survey coupled with transcriptome.
abstract: Hematopoietic stem cells are capable of self-renewal and differentiation into different hematopoietic lineages. To gain a comprehensive understanding of hematopoietic stem/progenitor cells, a systematic proteomic survey of human CD34+ cells collected from human umbilical cord blood was performed, in which the proteins were separated by 1- and 2-DE, as well as by nano-LC, and subsequently identified by MS. A total of 370 distinct proteins identified from those cells provided new insights into the potential of the stem/progenitor cells because the nerve, gonad, and eye-associated proteins were reliably identified. Interestingly, the transcripts of 133 (35.9%) identified proteins were not found by the prevalent transcriptome approaches, although several selected transcripts could be detected by RT-PCR. Moreover, the heterogeneity of 33 proteins identified from 2-DE was attributable primarily to post-translational processes rather than to alternative splicing at transcriptional level. Furthermore, the biosyntheses of 15 proteins identified in this study appears not to be completely interrupted in spite of the fact that corresponding antisense RNAs were found in the existing transcriptome data. The integrated proteomic and transcriptomic analyses employed here provided a unique view of the human stem/progenitor cells.
authors.affiliation: Chinese National Human Genome Center at Shanghai, Shanghai, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Jiong
authors.initials: J
authors.lastname: Lu
authors.forename: Hua-Hua
authors.initials: HH
authors.lastname: Fan
authors.forename: Zhi-Qin
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Shu-Jian
authors.initials: SJ
authors.lastname: Cui
authors.forename: Guo-An
authors.initials: GA
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Chi
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Peng-Yuan
authors.initials: PY
authors.lastname: Yang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Ze-Guang
authors.initials: ZG
authors.lastname: Han
mesh_terms: D018952:Antigens, CD34 / Q000096:biosynthesis; D002458:Cell Fractionation; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000145:classification*; D020543:Proteome / Q000737:chemistry* / Q000235:genetics; D040901:Proteomics / Q000379:methods; D016372:RNA, Antisense / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D018952:Antigens, CD34; D011506:Proteins; D020543:Proteome; D016372:RNA, Antisense