Molecular structure and organization of the wheat genomic manganese superoxide dismutase gene.
The genomic structure of a manganese superoxide dismutase (MnSOD) gene in wheat was elucidated by sequencing a clone from a BAC library of a stripe rust resistant wheat line. The clone was identified by hybridization with a wheat MnSOD cDNA. The gene consisted of 6 exons interrupted by 5 introns with a total length of 4770 nucleotides from the start codon to the termination codon. The wheat MnSOD gene was the longest among those sequenced from plant species. The transcription initiation site was preceded by a G+C-rich promoter without a TATA or CAAT box. The promoter contained many putative cis-acting regulatory elements, including an abscisic acid (ABA)-responsive element, a stress-responsive element, and a GC-repeat, as well as several other structural features in common with the promoter of the rice MnSOD gene. A Stowaway-like transposable element was found in intron 5 of the wheat MnSOD gene, but further investigation revealed the transposable element was not present in all copies of the MnSOD genes.
Baek
Kwang-Hyun
KH
Department of Crop and Soil Sciences, 210 Johnson Hall, Washington State University, Pullman, WA 99164-6420, USA.
Skinner
Daniel Z
DZ
Ling
Peng
P
Chen
Xianming
X
D001483:Base Sequence; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant; D016384:Consensus Sequence; D004251:DNA Transposable Elements; D018744:DNA, Plant / Q000032:analysis; D005091:Exons; D015723:Gene Library; D018745:Genome, Plant; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics*; D014908:Triticum / Q000201:enzymology* / Q000235:genetics*
D004251:DNA Transposable Elements; D018744:DNA, Plant; D013482:Superoxide Dismutase