Expression of the Arabidopsis high-affinity hexose transporter STP13 correlates with programmed cell death.
We report the biochemical characterization in Xenopus oocytes of the Arabidopsis thaliana membrane protein, STP13, as a high affinity, hexose-specific H(+)-symporter. Studies with kinase activators suggest that it is negatively regulated by phosphorylation. STP13 promoter GFP reporter lines show GFP expression only in the vascular tissue in emerging petals under non-stressed conditions. Quantitative PCR and the pSTP13-GFP plants show induction of STP13 in programmed cell death (PCD) obtained by treatments with the fungal toxin fumonisin B1 and the pathogen Pseudomonas syringae. A role for STP13 in PCD is supported by microarray data from e.g. plants undergoing senescence and a strong correlation between STP13 transcripts and the PCD phenotype in different accelerated cell death (acd11) mutants.
Norholm
Morten H H
MH
Plant Biochemistry Laboratory, center for Molecular Plant Physiology, the royal Veterinary and Agricultural University, Fredericksberg C, Denmark.
Nour-Eldin
Hussam H
HH
Brodersen
Peter
P
Mundy
John
J
Halkier
Barbara A
BA
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D037341:Fumonisins / Q000494:pharmacology; D015870:Gene Expression; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009865:Oocytes; D027981:Symporters / Q000096:biosynthesis* / Q000235:genetics; D014981:Xenopus
C451029:ACD11 protein, Arabidopsis; D051017:Apoptosis Regulatory Proteins; D029681:Arabidopsis Proteins; D004791:Enzyme Inhibitors; D037341:Fumonisins; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; C510622:STP13 protein, Arabidopsis; D027981:Symporters; C056933:fumonisin B1