Cardiolipin in hydrogenosomes: evidence of symbiotic origin.
Hydrogenosomes are found in organisms that lack typical mitochondria. Cardiolipin is a phospholipid located exclusively in bacterial membranes and the inner membrane of mitochondria. Here we show, by cell fractionation, thin-layer chromatography, high-pressure liquid chromatography, and matrix-assisted laser desorption ionization-time of flight mass spectrometry that hydrogenosomes of Tritrichomonas foetus, a cattle vaginal parasite, contain cardiolipin, which is strong evidence for its endosymbiotic origin.
de Andrade Rosa
Ivone
I
Laboratorio de Ultraestrutura Celular, Univeridade Santa Ursula, Rio de Janeiro, Brazil.
Einicker-Lamas
Marcelo
M
Roney Bernardo
Róbson
R
Previatto
Lucia Mendonça
LM
Mohana-Borges
Ronaldo
R
Morgado-Díaz
José Andrés
JA
Benchimol
Marlene
M
D000818:Animals; D005075:Biological Evolution; D002308:Cardiolipins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D015388:Organelles / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013559:Symbiosis; D014244:Trichomonas / Q000737:chemistry*
D002308:Cardiolipins