Splenectomy impairs diffusive oxygen transport in the lung of dogs.
The spleen acts as an erythrocyte reservoir in highly aerobic species such as the dog and horse. Sympathetic-mediated splenic contraction during exercise reversibly enhances convective O2 transport by increasing hematocrit, blood volume, and O2-carrying capacity. Based on theoretical interactions between erythrocytes and capillary membrane (Hsia CCW, Johnson RL Jr, and Shah D. J Appl Physiol 86: 1460-1467, 1999) and experimental findings in horses of a postsplenectomy reduction in peripheral O2-diffusing capacity (Wagner PD, Erickson BK, Kubo K, Hiraga A, Kai M, Yamaya Y, Richardson R, and Seaman J. Equine Vet J 18, Suppl: 82-89, 1995), we hypothesized that splenic contraction also augments diffusive O2 transport in the lung. Therefore, we have measured lung diffusing capacity (DL(CO)) and its components during exercise by a rebreathing technique in six adult foxhounds before and after splenectomy. Splenectomy eliminated exercise-induced polycythemia, associated with a 30% reduction in maximal O2 uptake. At any given pulmonary blood flow, DL(CO) was significantly lower after splenectomy owing to a lower membrane diffusing capacity, whereas pulmonary capillary blood volume changed variably; microvascular recruitment, indicated by the slope of the increase in DL(CO) with respect to pulmonary blood flow, was also reduced. We conclude that splenic contraction enhances both convective and diffusive O2 transport and provides another compensatory mechanism for maintaining alveolar O2 transport in the presence of restrictive lung disease or ambient hypoxia.
Dane
D Merrill
DM
Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390-9034, USA.
Hsia
Connie C W
CC
Wu
Eugene Y
EY
Hogg
Richard T
RT
Hogg
Deborah C
DC
Estrera
Aaron S
AS
Johnson
Robert L
RL
D000818:Animals; D001810:Blood Volume / Q000502:physiology; D002248:Carbon Monoxide / Q000097:blood; D004285:Dogs / Q000502:physiology*; D004911:Erythrocyte Volume / Q000502:physiology; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D000860:Hypoxia / Q000097:blood / Q000503:physiopathology; D008168:Lung / Q000098:blood supply / Q000502:physiology*; D008297:Male; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology; D011086:Polycythemia / Q000097:blood / Q000503:physiopathology; D011653:Pulmonary Diffusing Capacity / Q000502:physiology*; D015657:Respiratory Transport / Q000502:physiology*; D012146:Rest / Q000502:physiology; D013154:Spleen / Q000502:physiology; D013156:Splenectomy
D006454:Hemoglobins; D002248:Carbon Monoxide; D010100:Oxygen