title: Expression pattern of Filamin-240 in Drosophila blood cells.
abstract: The expression pattern of Filamin-240 was studied in subsets of Drosophila blood cells by means of immunofluorescent staining and Western blot analysis with use of an antibody specific to a "filamin-folding domain", a consensus motif profile generated from the 20 existing filamin repeats. Expression of Filamin-240 is restricted to lamellocytes - a special blood cell type of the cellular immune response - and is involved in the regulation of lamellocyte development. In the cher1 homozygous larvae, which lack Filamin-240 protein, a vigorous lamellocyte differentiation occurs which is further enhanced upon in vivo immune challenge by a parasitic wasp, Leptopilina boulardi. By introducing a full-length transgene encoding the Drosophila Filamin-240 protein into the cher1 Filamin-deficient homozygous mutant, the mutant blood cell phenotype was rescued. These data demonstrate that the expression of Filamin-240 is strictly lamellocyte specific in Drosophila blood cells and that the protein is a suppressor of lamellocyte development.
authors.affiliation: Biological Research Center of the Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62, Hungary. tina@brc.hu
authors.forename: Florentina
authors.initials: F
authors.lastname: Rus
authors.forename: Eva
authors.initials: E
authors.lastname: Kurucz
authors.forename: Róbert
authors.initials: R
authors.lastname: Márkus
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Sinenko
authors.forename: Barbara
authors.initials: B
authors.lastname: Laurinyecz
authors.forename: Csilla
authors.initials: C
authors.lastname: Pataki
authors.forename: János
authors.initials: J
authors.lastname: Gausz
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Hegedus
authors.forename: Andor
authors.initials: A
authors.lastname: Udvardy
authors.forename: Dan
authors.initials: D
authors.lastname: Hultmark
authors.forename: István
authors.initials: I
authors.lastname: Andó
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001773:Blood Cells / Q000166:cytology / Q000378:metabolism* / Q000469:parasitology; D002454:Cell Differentiation / Q000276:immunology; D003285:Contractile Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018076:DNA, Complementary / Q000302:isolation & purification; D004330:Drosophila / Q000254:growth & development / Q000378:metabolism* / Q000469:parasitology; D064448:Filamins; D020869:Gene Expression Profiling; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism; D007107:Immune System / Q000166:cytology / Q000378:metabolism; D007814:Larva / Q000166:cytology / Q000378:metabolism / Q000469:parasitology; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011485:Protein Binding; D020033:Protein Isoforms; D014018:Tissue Distribution; D014863:Wasps / Q000276:immunology
chemical_list: D003285:Contractile Proteins; D018076:DNA, Complementary; D064448:Filamins; D008840:Microfilament Proteins; D020033:Protein Isoforms