Brain amyloid accumulates in aged rats with kaolin-induced hydrocephalus.
Amyloid beta-peptide (Abeta) accumulation in aged Sprague-Dawley rats (12 months) with kaolin-induced hydrocephalus was investigated by Abeta(1-40) and Abeta(1-42) immunohistochemistry at 2, 6 and 10 weeks after induction. The low-density lipoprotein receptor-related protein-1 transporting Abeta across the blood-brain barrier was assayed. Age-matched controls showed some positive Abeta staining, mainly in the choroid plexus. At 2 weeks after induction, Abeta staining of the arachnoid and subependymal layer was observed. At 6 weeks, larger Abeta accumulations were prominent at the endothelial and perivascular sites. Intraparenchymal Abeta positively stained accumulations occurred at 10 weeks. Microvessel lipoprotein receptor-related protein-1 staining was progressively reduced from 2 to 10 weeks. The pattern of Abeta deposition and lipoprotein receptor-related protein-1 loss suggests reduced Abeta clearance in chronic hydrocephalus.
Klinge
Petra M
PM
International Neuroscience Institute Hannover, Hannover, Germany. klinge@ini-hannover.de
Samii
Amir
A
Niescken
Sabine
S
Brinker
Thomas
T
Silverberg
Gerald D
GD
D000375:Aging / Q000378:metabolism*; D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006849:Hydrocephalus / Q000139:chemically induced / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D007616:Kaolin / Q000633:toxicity*; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000682:Amyloid; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D007616:Kaolin