Enzymatic synthesis of prebiotic oligosaccharides.
Prebiotic oligosaccharides are nondigestible carbohydrates that can be obtained by enzymatic synthesis. Glucosyltransferases can be used to produce these carbohydrates through an acceptor reaction synthesis. When maltose is the acceptor a trisaccharide composed of one maltose unit and one glucose unit linked by an alpha-1,6-glycosidic bond (panose) is obtained as the primer product of the dextransucrase acceptor reaction. In this work, panose enzymatic synthesis was evaluated by a central composite experimental design in which maltose and sucrose concentration were varied in a wide range of maltose/sucrose ratios in a batch reactor system. A partially purified enzyme was used in order to reduce the process costs, because enzyme purification is one of the most expensive steps in enzymatic synthesis. Even using high maltose/sucrose ratios, dextran and higher-oligosaccharide formation were not avoided. The results showed that intermediate concentrations of sucrose and high maltose concentration resulted in high panose productivity with low dextran and higher-oligosaccharide productivity.
Rabelo
Maria C
MC
Departamento de Tecnologia de Alimentos, Universidade Federal do Ceara, Av. Mister Hull, 2977, bloco 858, Campus do Pici, CEP 60356-000 Fortaleza, CE, Brazil.
Honorato
Talita L
TL
Gonçalves
Luciana R B
LR
Pinto
Gustavo A S
GA
Rodrigues
Sueli
S
D001426:Bacterial Proteins / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry; D016695:Glycosyltransferases / Q000737:chemistry*; D007934:Leuconostoc / Q000201:enzymology*; D008320:Maltose; D009844:Oligosaccharides / Q000138:chemical synthesis*; D013395:Sucrose / Q000737:chemistry
D001426:Bacterial Proteins; D005936:Glucans; D009844:Oligosaccharides; C008763:panose; D013395:Sucrose; D008320:Maltose; D016695:Glycosyltransferases