title: Culture conditions of Roseobacter strain 27-4 affect its attachment and biofilm formation as quantified by real-time PCR.
abstract: The fish probiotic bacterium Roseobacter strain 27-4 grows only as rosettes and produces its antibacterial compound under static growth conditions. It forms three-dimensional biofilms when precultured under static conditions. We quantified attachment of Roseobacter strain 27-4 using a direct real-time PCR method and demonstrated that the bacteria attached more efficiently to surfaces during static growth than under aerated conditions.
authors.affiliation: Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, DTU Bldg. 221, DK-2800 Kongens Lyngby, Denmark. jbb@dfu.min.dk
authors.forename: Jesper Bartholin
authors.initials: JB
authors.lastname: Bruhn
authors.forename: Janus Anders Juul
authors.initials: JA
authors.lastname: Haagensen
authors.forename: Dorthe
authors.initials: D
authors.lastname: Bagge-Ravn
authors.forename: Lone
authors.initials: L
authors.lastname: Gram
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D011137:Polystyrenes; D041904:Roseobacter / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D011137:Polystyrenes