Kinetic analysis of the ex vivo expansion of human hematopoietic stem/progenitor cells.
The total cell expansion of human umbilical cord blood (CB) and adult bone marrow (BM) CD34+-enriched cells cultured in supplemented serum-free media, either over irradiated human feeder layers or in stroma-free systems, were characterized by a simple kinetic model using only two parameters: the specific cell expansion rate, mu, and the death rate constant, k(k). Both CB and BM cells can expand at approximately the same rate (0.21 day(-1)) in this culture system however, cell death depends on the presence of stroma and the environment in which the cells are cultured.
Gonçalves
Raquel
R
Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisboa, Portugal.
da Silva
Cláudia Lobato
CL
Ferreira
Bruno S
BS
Lemos
Maria Amélia N D A
MA
Lemos
Francisco
F
Almeida-Porada
Graça
G
Cabral
Joaquim M S
JM
D017209:Apoptosis / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D003198:Computer Simulation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D023822:Tissue Engineering / Q000379:methods*