Control of drug accessibility on functional polyelectrolyte multilayer films.
A surface coating based on polylysine/hyaluronic acid multilayers was designed and acted as a reservoir for an antiproliferative agent, paclitaxel (Taxol). Absolutely no chemical modification of polyelectrolytes or of the drug was needed and the final architecture was obtained in an extremely simple way using the layer-by-layer method. The paclitaxel dose available for human colonic adenocarcinoma cells HT29 seeded on the films could be finely tuned. Moreover, the accessibility of the drugs was controlled by adding on the top of the drug reservoir a capping made of synthetic polyelectrolyte multilayers. This capping was also required to allow adhesion of HT29 cells. Paclitaxel activity was maintained after embedding in the polyelectrolyte multilayers and cellular viability could be reduced by about 80% 96 h after seeding. The strategy described in this paper could be valuable for various other drug/cell systems.
Vodouhê
Constant
C
INSERM Unité 595, 11 rue Humann, F-67085 Strasbourg Cedex, France. constant.vodouhe@odonto-ulp.u-strasbg.fr
Le Guen
Erell
E
Garza
Juan Mendez
JM
Francius
Gregory
G
Déjugnat
Christophe
C
Ogier
Joëlle
J
Schaaf
Pierre
P
Voegel
Jean-Claude
JC
Lavalle
Philippe
P
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002470:Cell Survival; D016503:Drug Delivery Systems; D004573:Electrolytes; D006801:Humans; D008422:Materials Testing / Q000379:methods; D018613:Microscopy, Confocal; D017239:Paclitaxel / Q000008:administration & dosage / Q000493:pharmacokinetics; D013499:Surface Properties; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods
D000972:Antineoplastic Agents, Phytogenic; D001672:Biocompatible Materials; D004573:Electrolytes; D017239:Paclitaxel