Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene.
We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F2 panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM.
Niimi
Teruyuki
T
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
Sahara
Ken
K
Oshima
Hiroyuki
H
Yasukochi
Yuji
Y
Ikeo
Kazuho
K
Traut
Walther
W
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000196:embryology / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004625:Embryo, Nonmammalian; D005091:Exons; D017344:Genes, Insect; D008040:Genetic Linkage; D016678:Genome; D017404:In Situ Hybridization, Fluorescence; D019476:Insect Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020033:Protein Isoforms
D018076:DNA, Complementary; D019476:Insect Proteins; D020033:Protein Isoforms; D004247:DNA