Degradation of plasticizer di-n-butylphthalate by Delftia sp. TBKNP-05.
Bacterial strain Delftia sp. TBKNP-05, isolated by para-hydroxybenzoate enrichment technique, is capable of degrading di-n-butylphthalate (DBP) as a sole source of carbon and energy. Analysis of intermediates by thin-layer chromatography and high-performance liquid chromatography indicated the presence of monobutylphthalate (MBP), phthalate (PA), and protocatechuate (PCA). The washed cells grown on DBP and PA showed appreciable oxidation of DBP, MBP, PA, and PCA. The enzyme activities in cell-free extracts of Delftia sp. TBKNP-05 exhibited the presence of DBP esterase, MBP esterase, PA-dioxygenase, and PCA 4,5-dioxygenase. The PCA is metabolized by meta-cleavage pathway, leading to further mineralization of the compound in this bacterium.
Patil
Neelakanteshwar K
NK
Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka, 585 106, India.
Kundapur
Rajesh
R
Shouche
Yogesh S
YS
Karegoudar
T B
TB
D001673:Biodegradation, Environmental; D003470:Culture Media; D004275:DNA, Ribosomal / Q000032:analysis; D042742:Delftia / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D003993:Dibutyl Phthalate / Q000378:metabolism*; D049308:Dioxygenases / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010795:Phthalic Acids / Q000378:metabolism; D010802:Phylogeny; D010968:Plasticizers / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004275:DNA, Ribosomal; D010795:Phthalic Acids; D010968:Plasticizers; D012336:RNA, Ribosomal, 16S; D003993:Dibutyl Phthalate; C032279:phthalic acid; D049308:Dioxygenases; D004950:Esterases