title: [With genechip technique to investigate HLA-DQB1 gene polymorphisms in south and north regions of China].
abstract: OBJECTIVE
  To research and compare HLA-DQB1 gene frequency(GF) and polymorphism distribution between south and north population of Chinese in China.
  
  
  METHODS
  Combining PCR-sequence specific primers(SSP) and sequence specific oligonucleotide probe(SSOP) techniques and DNA Microarray Kit for HLA-DQB1 Low Res Genotyping from Shenzhen Yi-Shengtang Biological LTD. Co. was used to type HLA-DQB1 gene polymorphisms of 700 individuals living in south China and 320 individuals in north China.
  
  
  RESULTS
  We inspected 10 alleles of HLA-DQB1 and got a series of comprehensive and accurate statistic data.
  
  
  CONCLUSIONS
  It is tested that HLA-DQB1*02, 05, 0601, 0602, 0603 gene frequencies are different obviously(P<0.05) between south and north Chinese. And those data will be useful to kinds of research associated with disease relevant and anthropology research.
authors.affiliation: Shenzhen Yi-Shengtang Biological LTD. Company, Shenzhen, Guangdong, 518054 P.R.China. wendyrabbit56@163.com
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Da-lin
authors.initials: DL
authors.lastname: Wu
authors.forename: A-dong
authors.initials: AD
authors.lastname: Shen
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Ya-qin
authors.initials: YQ
authors.lastname: Liu
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D015345:Oligonucleotide Probes