[Differential trypan blue staining of tumor cells for the determination of apoptosis].
The present paper suggests that trypan blue should be used for differential staining of tumor cells in order to determine apoptosis. The proposed method makes it possible to simplify operations, to reduce the time required to carry out a test, and to enhance the precision of determining the course of apoptosis during routine investigations. The paper also gives data of the comparative studies of the content of apoptotic cells in experimental melanoma B-16 though differential trypan blue staining and classical morphostructural analysis. The difference in the values obtained by these two methods is shown to be 35%.
Abduvaliev
A A
AA
Gil'dieva
M S
MS
D017209:Apoptosis; D001943:Breast Neoplasms / Q000473:pathology*; D004396:Coloring Agents; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008545:Melanoma / Q000473:pathology*; D014343:Trypan Blue
D004396:Coloring Agents; D014343:Trypan Blue