A novel ribonuclease from fresh fruiting bodies of the portabella mushroom Agaricus bisporus.
A 14 kDa ribonuclease with a novel N-terminal sequence was isolated from fresh fruiting bodies of the portabella mushroom. It was adsorbed on DEAE-cellulose and carboxymethyl-cellulose, and demonstrated the highest ribonucleolytic potency toward poly (A), 60% as much activity toward poly (C), 40% as much activity toward poly (U), and the least activity (7% as much) toward poly (G). It exhibited a pH optimum at pH 4.5 and a temperature optimum at 60 degrees C. Its activity at 100 degrees C was higher than that at 20 degrees C.
Wang
H X
HX
Department of Microbiology, China Agricultural University, Beijing and State Key Laboratory for Agrobiotechnology, China.
Ng
T B
TB
D000364:Agaricus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D000595:Amino Acid Sequence; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012260:Ribonucleases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D012260:Ribonucleases