title: Cloning and characterization of a cDNA encoding cholesterol side-chain cleavage cytochrome P450 (CYP11A1): tissue-distribution and changes in the transcript abundance in ovarian tissue of Japanese eel, Anguilla japonica, during artificially induced sexual development.
abstract: Cholesterol side-chain cleavage cytochrome P450 (CYP11A1: P450scc) is a crucial steroidogenic enzyme that catalyzes an initial step in the production of all classes of steroids. A cDNA encoding Japanese eel P450scc was cloned and characterized. The cDNA putatively encoded 521 amino acid residues with high homology to those of other vertebrate forms. The recombinant P450scc produced in COS-7 cells efficiently catalyzed the conversion of 25-hydroxycholesterol into pregnenolone. By northern blot, a single P450scc transcript of approximately 3.3 kb was detected in both ovary and head kidney. Transcript levels of this enzyme significantly increased throughout ovarian development artificially induced by salmon pituitary homogenate, which suggests that gonadotropic stimuli can induce ovarian expression of the P450scc gene in teleosts, as has been reported in mammals. Furthermore, RT-PCR analysis revealed that gene expression of three steroidogenic enzymes, P450scc, P450c17 and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) show distinctly different tissue-specific patterns of expression in the Japanese eel. The P450scc gene was expressed in ovary and head kidney while the sole source of the P450c17 transcript was ovary. In contrast, 3beta-HSD transcript was detected in all tissues examined, brain, liver, spleen and trunk kidney, etc. These suggest that some steroidogenic enzymes are also expressed in non-endocrine tissues and could potentially regulate the local and/or circulating steroid levels in teleosts, as they do in mammals.
authors.affiliation: Division of Marine Life Sciences, Research Faculty of Fisheries Science, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan. kazeto@fish.hokudai.ac.jp
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Kazeto
authors.forename: Shigeho
authors.initials: S
authors.lastname: Ijiri
authors.forename: Shinji
authors.initials: S
authors.lastname: Adachi
authors.forename: Kohei
authors.initials: K
authors.lastname: Yamauchi
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics; D000595:Amino Acid Sequence; D000811:Anguilla / Q000235:genetics* / Q000254:growth & development; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006888:Hydroxycholesterols / Q000378:metabolism; D008969:Molecular Sequence Data; D010053:Ovary / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D011284:Pregnenolone / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D046468:Sexual Development / Q000235:genetics; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D006888:Hydroxycholesterols; D011284:Pregnenolone; C007997:25-hydroxycholesterol; D015096:3-Hydroxysteroid Dehydrogenases; D013254:Steroid 17-alpha-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme