title: [The effects of different transcripts of AML1 on the transactivation of M-CSF-R gene].
abstract: OBJECTIVE
  To observe the effects of AML1A and AML1B, two splicing isoforms of AML1, on the transactivation of macrophage colony-stimulating factor receptor (M-CSF-R), and explore the mechanism of hematopoietic stem cell committed differentiation and leukemogenesis.
  
  
  METHODS
  The expressive plasmids of AML1A and AML1B were constructed, and co-transfected into CV-1 cells with a luciferase reporter plasmid containing M-CSF-R promoter. The transactivity of M-CSF-R promoter was assayed by luminometer.
  
  
  RESULTS
  AML1B exhibited a distinct transactivity to M-CSF-R promoter with a sequence-specificity and dosage-dependent manner. AML1A showed no any transactivity but antagonized the effect of AML1B, causing marked reduction of M-CSF-R expression.
  
  
  CONCLUSIONS
  An intact structure of AML1 is necessary for transactivation of M-CSF-R. AML1A may interfere with the transactivation of AML1B, and play a key role in the fine regulation of committed differentiation of hematopoietic cell.
authors.affiliation: Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Xing
authors.forename: Qing
authors.initials: Q
authors.lastname: Rao
authors.forename: Jian-xiang
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic; D005822:Genetic Vectors; D000882:Haplorhini; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007668:Kidney / Q000166:cytology; D010957:Plasmids / Q000235:genetics; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D016186:Receptor, Macrophage Colony-Stimulating Factor