Effective therapy of transected quadriceps muscle in rat: Gastric pentadecapeptide BPC 157.
We report complete transection of major muscle and the systemic peptide treatment that induces healing of quadriceps muscle promptly and then maintains the healing with functional restoration. Initially, stable gastric pentadecapeptide BPC 157 (GEPPPGKPADDAGLV, M.W. 1419, PL-10, PLD-116, PL 14736 Pliva, Croatia; in trials for inflammatory bowel disease; wound treatment; no toxicity reported; effective alone without carrier) also superiorly accelerates the healing of transected Achilles tendon. Regularly, quadriceps muscle completely transected transversely 1.0 cm proximal to patella presents a definitive defect that cannot be compensated in rat. BPC 157 (10 microg, 10 ng, 10 pg/kg) is given intraperitoneally, once daily; the first application 30 min posttransection, the final 24 h before sacrifice. It consistently improves muscle healing throughout the whole 72-day period. Improved are: (i) biomechanic (load of failure increased); (ii) function (walking recovery and extensor postural thrust/motor function index returned toward normal healthy values); (iii) microscopy/immunochemistry [i.e., mostly muscle fibers connect muscle segments; absent gap; significant desmin positivity for ongoing regeneration of muscle; larger myofibril diameters on both sides, distal and proximal (normal healthy rat-values reached)]; (iv) macroscopic presentation (stumps connected; subsequently, atrophy markedly attenuated; finally, presentation close to normal noninjured muscle, no postsurgery leg contracture). Thus, posttransection healing-consistently improved-may suggest this peptide therapeutic application in muscle disorders.
Staresinic
Mario
M
Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, P.O.B. 916, 10000 Zagreb, Croatia.
Petrovic
Igor
I
Novinscak
Tomislav
T
Jukic
Ivana
I
Pevec
Damira
D
Suknaic
Slaven
S
Kokic
Neven
N
Batelja
Lovorka
L
Brcic
Luka
L
Boban-Blagaic
Alenka
A
Zoric
Zdenka
Z
Ivanovic
Domagoj
D
Ajduk
Marko
M
Sebecic
Bozidar
B
Patrlj
Leonardo
L
Sosa
Tomislav
T
Buljat
Gojko
G
Anic
Tomislav
T
Seiwerth
Sven
S
Sikiric
Predrag
P
D000595:Amino Acid Sequence; D000818:Animals; D001696:Biomechanical Phenomena; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000293:injuries* / Q000503:physiopathology; D010446:Peptide Fragments / Q000627:therapeutic use*; D011506:Proteins / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013708:Tendon Injuries / Q000188:drug therapy* / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects*
D010446:Peptide Fragments; D011506:Proteins; C083833:BPC 157