title: Acid inactivation of prions: efficient at elevated temperature or high acid concentration.
abstract: Scrapie prion rods isolated from hamster and non-infectious aggregates of the corresponding recombinant protein rPrP(90-231) were incubated with hydrochloric acid. The amount of PrP and of infectivity that survived incubation in HCl at varying times, acid concentrations and temperatures was quantified by Western blot densitometry and bioassays, respectively. Prion rods and rPrP aggregates showed similar HCl hydrolysis kinetics of PrP, indicating structural homology. For 1 M HCl and 25 degrees C, the rate of PrP hydrolysis follows first-order kinetics at 0.014 h(-1); the rate of infectivity inactivation is 0.54 h(-1). Hydrolysis for 1 h at 25 degrees C was only slightly proportional to HCl concentration up to 5 M, but complete loss of infectivity and PrP reduction to <2 % was observed at 8 M HCl. The temperature dependence of unhydrolysed PrP, as well as infectivity at 1 M HCl for 1 h, showed a slight decrease up to 45 degrees C, but a sigmoidal decrease by several orders of magnitude at higher temperatures. The slow hydrolysis of PrP and inactivation of infectivity by acid treatment at room temperature are attributed to solvent inaccessibility of prion rods and rPrP aggregates, respectively. The more effective hydrolysis and inactivation at temperatures above 45 degrees C are interpreted as thermally induced disaggregation with an activation energy of 50-60 kJ mol(-1). Most importantly, infectivity was always inactivated faster or to a higher extent than PrP was hydrolysed at several incubation times, HCl concentrations and temperatures.
authors.affiliation: Institut für Physikalische Biologie, Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universität Düsseldorf, Gebäude 26.12, D-40225 Düsseldorf, Germany.|IMB, Leibniz Institut für Molekulare Biotechnologie Jena eV, Jena, Germany.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Appel
authors.affiliation: Institut für Physikalische Biologie, Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universität Düsseldorf, Gebäude 26.12, D-40225 Düsseldorf, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Lucassen
authors.affiliation: Friedrich-Löffler-Institut, Greifswald Insel Riems, Germany.
authors.forename: Martin H
authors.initials: MH
authors.lastname: Groschup
authors.affiliation: Robert Koch-Institut, P24-TSE, Berlin, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Joncic
authors.affiliation: Robert Koch-Institut, P24-TSE, Berlin, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Beekes
authors.affiliation: Institut für Physikalische Biologie, Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universität Düsseldorf, Gebäude 26.12, D-40225 Düsseldorf, Germany.
authors.forename: Detlev
authors.initials: D
authors.lastname: Riesner
mesh_terms: D000818:Animals; D006224:Cricetinae; D006851:Hydrochloric Acid / Q000494:pharmacology*; D006868:Hydrolysis; D007700:Kinetics; D018620:PrPSc Proteins / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D011994:Recombinant Proteins / Q000187:drug effects; D013696:Temperature
chemical_list: D018620:PrPSc Proteins; D011994:Recombinant Proteins; D006851:Hydrochloric Acid