cDNA macroarray for analysis of gene expression profiles in prostate cancer.
BACKGROUND
Early diagnosis and timely treatment are important for improving therapeutic efficiency of prostate cancer. DNA array is a new bio-technology for disease diagnosis. This study was conducted to diagnose prostate cancer with cDNA macroarray and analysis gene expression profiles of some selective genes in prostate cancer.


METHODS
Total RNA was isolated from patients with prostate cancer and from normal people, and poly (A) RNA was further purified. Then it was analyzed for differentially expressed genes in prostate cancer and normal prostate by cDNA macroarray system.


RESULTS
There were different expressions in the nine prostate-associated specific genes in prostate cancer as compared with normal prostate, in which, 7 were significantly upregulated and 2 were down-regulated.


CONCLUSIONS
As a diagnostic approach at molecular level, the cDNA macroarray is an effectively diagnostic method for prostate cancer.
Zhong
Wei-de
WD
Department of Urology, Guangzhou First Municipal People's Hospital, Guangzhou Medical College, Guangzhou 510180, China. wdezhong@21cn.com
He
Hui-chan
HC
Bi
Xue-cheng
XC
Ou
Ru-biao
RB
Jiang
Shao-ai
SA
Liu
Liang-shi
LS
D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics*
D017430:Prostate-Specific Antigen