Aspects of large-scale chromatin structures in mouse liver nuclei can be predicted from the DNA sequence.
The large amount of non-coding DNA present in mammalian genomes suggests that some of it may play a structural or functional role. We provide evidence that it is possible to predict computationally, from the DNA sequence, loci in mouse liver nuclei that possess distinctive nucleosome arrays. We tested the hypothesis that a 100 kb region of DNA possessing a strong, in-phase, dinucleosome period oscillation in the motif period-10 non-T, A/T, G, should generate a nucleosome array with a nucleosome repeat that is one-half of the dinucleosome oscillation period value, as computed by Fourier analysis of the sequence. Ten loci with short repeats, that would be readily distinguishable from the pervasive bulk repeat, were predicted computationally and then tested experimentally. We estimated experimentally that less than 20% of the chromatin in mouse liver nuclei has a nucleosome repeat length that is 15 bp, or more, shorter than the bulk repeat value of 195 +/- bp. All 10 computational predictions were confirmed experimentally with high statistical significance. Nucleosome repeats as short as 172 +/- 5 bp were observed for the first time in mouse liver chromatin. These findings may be useful for identifying distinctive chromatin structures computationally from the DNA sequence.
Cioffi
Alfred
A
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Fleury
Tomara J
TJ
Stein
Arnold
A
D000818:Animals; D019295:Computational Biology; D023281:Genomics; D008099:Liver / Q000737:chemistry*; D051379:Mice; D009707:Nucleosomes / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*
D009707:Nucleosomes