EST-based identification of genes expressed in the liver of adult seabass (Dicentrarchus labrax, L.).
The scarcity of the genomic resources for some fish species, in spite of their commercial interest, could retard the positive effects that modern biotechnology can offer to aquaculture industry. Then an effort should be made to reduce, as far as it concerns genomic resources, the gap that separates farming species from "model organisms". In this paper, we present an EST project in which we performed single pass sequencing on 1229 randomly selected clones from a sea bass cDNA library. The sequences are deposited in the NCBI database with the following accession numbers: from , from , from and from . EST cataloguing and profiling of seabass will set the basis for functional genomic research in this species, but will also serve for comparative and environmental genomics, for the identification of polymorphic markers useful, for example, to survey the disease resistance of fish, for the discovery of new molecular markers of exposure and for the production of micro- and macro-arrays.
Chini
Valentina
V
Dipartimento di Biotecnologie e Scienze Molecolari, Università dell'Insubria, 3 Via Dunant, I-21100 Varese, Italy.
Rimoldi
Simona
S
Terova
Genciana
G
Saroglia
Marco
M
Rossi
Federica
F
Bernardini
Giovanni
G
Gornati
Rosalba
R
D000818:Animals; D001483:Base Sequence / Q000235:genetics*; D001492:Bass / Q000235:genetics*; D018076:DNA, Complementary; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D008099:Liver; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary