Simultaneous determination of pancuronium, vecuronium and their related compounds using LC-ESI-MS.
A simultaneous determination method of quaternary amino steroidal muscle relaxants, pancuronium (PAN), vecuronium (VEC), and 17-monodesacetyl pancuronium (17-OH-PAN), 3,17-bisdesacetyl pancuronium (3,17-OH-PAN), 3-monodesacetyl vecuronium (3-OH-VEC), 3,17-bisdesacetyl vecuronium (3,17-OH-VEC) in human serum was developed using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The weak cation exchange cartridge was useful for the extraction of these compounds. Under optimized LC-ESI-MS conditions, these compounds were almost fully separated within 6.5 min. Linear responses over the concentration range 0.25-50.0 ng/mL were demonstrated for each compound. The developed method successfully detected VEC, 3-OH-VEC and 3,17-OH-VEC in serum intravenously administered with VEC. The level of 3-OH-VEC was higher than other compounds. This suggested that 3-OH-VEC was useful as a forensic probe in VEC administration.
Usui
Kiyotaka
K
Division of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Hishinuma
Takanori
T
Yamaguchi
Hiroaki
H
Saga
Toshihide
T
Wagatsuma
Toshihiro
T
Hoshi
Kunihiko
K
Tachiiri
Naoki
N
Miura
Kuniaki
K
Goto
Junichi
J
D002853:Chromatography, Liquid; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D003473:Neuromuscular Nondepolarizing Agents / Q000097:blood*; D010197:Pancuronium / Q000031:analogs & derivatives / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization; D014673:Vecuronium Bromide / Q000031:analogs & derivatives / Q000097:blood*
C515389:17-(monodesacetyl)pancuronium; D003473:Neuromuscular Nondepolarizing Agents; C056450:(dideacetoxy)vecuronium; C056447:(dideacetoxy)pancuronium; D014673:Vecuronium Bromide; C056449:3-(deacetoxy)vecuronium; D010197:Pancuronium