Molecular identity of the electrophoretic pattern and the major cross-reacting immunogens of Toxoplasma gondii (RH strain) and Neospora caninum.
The complex electrophoretic profile of the antigenic components of Toxoplasma gondii and Neospora caninum was studied. T. gondii (RH strain) trophozoite's antigens were resolved by gel electrophoresis under denaturating condition. Resolved polypeptides were tested by immunoblotting against hyper-immune serum prepared in Boscat rabbits. Immunoblot analysis revealed 6 reactive bands, which are 158, 111, 102, 86, 55 and 33 kDa, out of 10 antigenic bands of the parasite. Concerning N. caninum trophozoite's antigens, immunoblot analysis revealed 5 reactive bands, which are 159, 119, 106, 97 & 39 kDa, out of 8 antigenic bands of the parasite. Cross reactivity trials between the 2 parasites explore 3 cross-reactive bands, indicating high antigenic similarity in between.
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Department of Parasitology, Animal Health Research Institute, Dokki, Giza, Egypt.
Barakat
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Hassan
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D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry* / Q000276:immunology; D003429:Cross Reactions; D004587:Electrophoresis, Agar Gel; D007106:Immune Sera; D008970:Molecular Weight; D018688:Neospora / Q000276:immunology*; D011817:Rabbits; D013045:Species Specificity; D014122:Toxoplasma / Q000276:immunology*
D000953:Antigens, Protozoan; D007106:Immune Sera