title: Engineering a rhodopsin protein mimic.
abstract: Due to the difficulties in handling and manipulating membrane-bound proteins, such as rhodopsin, and the lack of crystallographic information on the cone opsins, we have opted to engineer a protein mimic of the transmembrane G-protein coupled receptor. Human cellular retinoic acid binding protein (CRABPII), a well studied and characterized protein, has been reengineered into a protein that now will bind retinal as a protonated Schiff base with high binding affinity (Kd = 2 nM) mimicking that of rhodopsin.
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: Rachael M
authors.initials: RM
authors.lastname: Crist
authors.forename: Chrysoula
authors.initials: C
authors.lastname: Vasileiou
authors.forename: Montserrat
authors.initials: M
authors.lastname: Rabago-Smith
authors.forename: James H
authors.initials: JH
authors.lastname: Geiger
authors.forename: Babak
authors.initials: B
authors.lastname: Borhan
mesh_terms: D001665:Binding Sites; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism; D018360:Crystallography, X-Ray; D006801:Humans; D007700:Kinetics; D008239:Lysine / Q000737:chemistry / Q000378:metabolism; D015202:Protein Engineering; D018168:Receptors, Retinoic Acid / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012243:Rhodopsin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014212:Tretinoin / Q000737:chemistry / Q000378:metabolism
chemical_list: D018168:Receptors, Retinoic Acid; C082928:retinoic acid binding protein II, cellular; D014212:Tretinoin; D012243:Rhodopsin; D008239:Lysine