title: Development of the rapid and simple elisa (whole blood-ELISA) using coconut water-tween as a wash solution for whole blood sample from Schistosoma japonicum-infected rabbit and human.
abstract: PBS-Tween as a wash solution, prepared with distilled water, is used in ELISA. In areas where schistosomiasis is endemic, however, distilled water is hard to come by. We have modified a WHOLE BLOOD-ELISA test to use coconut water-Tween as a wash solution, because coconut water is easy to come by and cheap in the tropics. We applied the test to whole blood samples from rabbits and humans infected with Schistosoma japonicum. This modified WHOLE BLOOD-ELISA was confirmed to be a rapid, simple, and cost-effective method.
authors.affiliation: Department of Tropical Medicine and Parasitology, Dokkyo University School of Medicine, Mibu, Shimotsuga, Tochigi, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Hirose
authors.forename: Ignacio L
authors.initials: IL
authors.lastname: Lipayon
authors.forename: Masashi
authors.initials: M
authors.lastname: Kirinoki
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Chigusa
authors.forename: Hajime
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D003059:Cocos; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008403:Mass Screening / Q000379:methods; D011817:Rabbits; D012549:Schistosoma japonicum / Q000201:enzymology / Q000302:isolation & purification*; D012552:Schistosomiasis / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology; D012698:Serologic Tests / Q000379:methods; D012996:Solutions
chemical_list: D012996:Solutions