Activating Gs a mutation rarely occurs in musculoskeletal tumors other than fibrous dysplasia.
BACKGROUND
Activating Gs a mutations have been identified in most instances of fibrous dysplasia (FD). This mutation leads to consistently elevated intracellular cyclic adenosine monophosphate (cAMP) levels, with various biological consequences. The development of secondary sarcoma in FD is a rare but well-established phenomenon. This finding raised the possibility that a common gene mutation exists in these tumors.


METHODS
The expression of the Gs a mutation was examined in 16 cell lines and 173 musculoskeletal tumor tissues, including 13 cases of FD, via RT-PCR and sequence analysis.


RESULTS
No expression of a Gs a mutation was detected in any cell line or clinical tissue sample, excluding FD tissues. Direct sequence analysis demonstrated results identical to those of RT-PCR.


CONCLUSIONS
Activating Gs a mutation rarely occurs in musculoskeletal tumors other than FD. The occurrence of most sarcomas displays no correlation with Gs a mutations.
Gu
Wenguang
W
Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata, 951-8510, Japan.
Ogose
Akira
A
Matsuba
Atsushi
A
Kawashima
Hiroyuki
H
Hotta
Tetsuo
T
Kudo
Naoko
N
Hoshino
Makiko
M
Kondo
Naoki
N
Mera
Hisashi
H
Endo
Naoto
N
D001859:Bone Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D005357:Fibrous Dysplasia of Bone / Q000235:genetics*; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000235:genetics*; D006801:Humans; D019042:Muscle Neoplasms / Q000235:genetics*; D009154:Mutation; D012516:Osteosarcoma / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012509:Sarcoma / Q000235:genetics*
D019205:GTP-Binding Protein alpha Subunits, Gs