[Study on molecular genetic structure of Ael blood subgroup].
OBJECTIVE
To study the ABO allele molecular characteristics of Ael blood subgroup.


METHODS
Five individuals of diagnosed as Ael blood subgroup were subjected to PCR amplify ABO alleles using four pairs of sequence-specific primers. Exon 6 and exon 7 at ABO locus of all samples were sequenced. An individual with AelB phenotype was chosen for further analysis of transcript structure of ABO gene.


RESULTS
Sequence analysis indicated one Ael phenotype sample with reported Ael01 allele, one Ael phenotype sample with an Ael05 allele, and two AelB and one Ael individuals did not contain referred A allele, but contain O01 or O02 allele with 261G deletion.


CONCLUSIONS
Molecular bases for the Ael have highly polymorphism. The mechanism responsible for the express weak A antigen of O allele with 261G deletion awaits to be elucidated.
Yu
Qiong
Q
Shenzhen Blood Centre, Shenzhen Institute of Transfusion Medicine, Shenzhen, Guangdong, 518035 P.R.China. yuqiong7867@yahoo.com
Wu
Guo-guang
GG
Liang
Yan-lian
YL
Deng
Zhi-hui
ZH
Su
Yu-qing
YQ
Wang
Da-ming
DM
D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D000017:ABO Blood-Group System; D004247:DNA