Regulation of expression of the arabinose and glucose transporter genes in the thermophilic archaeon Sulfolobus solfataricus.
Sugar uptake in Sulfolobus solfataricus, a thermoacidophilic archaeon, occurs through high-affinity binding of protein-dependent ABC transporters. We have investigated the expression patterns of two sugar transport operons, that is, the glucose and arabinose transporters. Analysis of the araS promoter activity, and the mRNA and protein levels in S. solfataricus cells grown on different carbon sources showed that expression of the arabinose transporter gene cluster is highly regulated and dependent on the presence of arabinose in the medium. Glucose in the growth medium repressed the expression of the arabinose transport genes. By means of primer extension, the transcriptional start site for the arabinose operon was mapped. Interestingly, expression of the arabinose transporter is down-regulated by addition of a selective set of amino acids to the medium. Expression of the glucose transporter genes appeared constitutive. These data confirm the earlier observation of a catabolite repression-like system in S. solfataricus.
Lubelska
Joanna M
JM
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands.
Jonuscheit
Melanie
M
Schleper
Christa
C
Albers
Sonja-Verena
SV
Driessen
Arnold J M
AJ
D000596:Amino Acids / Q000494:pharmacology; D001089:Arabinose / Q000378:metabolism*; D019843:Archaeal Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics; D019848:Gene Expression Regulation, Archaeal / Q000187:drug effects; D019766:Genes, Archaeal; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism; D009876:Operon; D011401:Promoter Regions, Genetic; D019642:RNA, Archaeal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D048229:Sulfolobus solfataricus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
D000596:Amino Acids; D019843:Archaeal Proteins; D015342:DNA Probes; D051246:Glucose Transport Proteins, Facilitative; D009004:Monosaccharide Transport Proteins; D019642:RNA, Archaeal; D012333:RNA, Messenger; D001089:Arabinose