title: Elevated levels of fragmented laminin-5 gamma2-chain in bronchoalveolar lavage fluid from dogs with pulmonary eosinophilia.
abstract: Inflammation causes epithelial cell sloughing and basement membrane (BM) exposure in canine pulmonary eosinophilia (PE), leading to degradation of the epithelial cell attachment component, laminin-5 gamma2-chain, into small molecular weight fragments. The subsidence of inflammation after treatment down-regulates degradation. Laminin-5 gamma2-chain levels and molecular forms in bronchoalveolar lavage fluid (BALF) were analysed semiquantitatively by Western immunoblotting to compare PE affected (n=20) and healthy dogs (n=16) as well as PE dogs (n=6) before and after corticosteroid treatment. PE dogs expressed significantly elevated levels of total (P<0.01), 36 kDa (P<0.05) and 53 kDa (P<0.05) laminin-5 gamma2-fragments. The 36 Da fragment decreased significantly (P<0.05) after treatment. The laminin-5 gamma2-chain degradation products may be linked to epithelial cell sloughing and BM exposure or healing.
authors.affiliation: Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, 00014 University of Helsinki, P.O. Box 57, Hämeentie 57, Helsinki, Finland. minna.m.rajamaki@helsinki.fi
authors.forename: Minna M
authors.initials: MM
authors.lastname: Rajamäki
authors.forename: Anna-Kaisa
authors.initials: AK
authors.lastname: Järvinen
authors.forename: Timo A
authors.initials: TA
authors.lastname: Sorsa
authors.forename: Taina I
authors.initials: TI
authors.lastname: Tervahartiala
authors.forename: Päivi S
authors.initials: PS
authors.lastname: Maisi
mesh_terms: D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D004283:Dog Diseases / Q000276:immunology / Q000378:metabolism*; D004285:Dogs; D007797:Laminin / Q000378:metabolism*; D008970:Molecular Weight; D011657:Pulmonary Eosinophilia / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D011897:Random Allocation
chemical_list: D000305:Adrenal Cortex Hormones; D007797:Laminin