title: Effect of pretreatment of salt, copper and temperature on ultraviolet-B-induced antioxidants in diazotrophic cyanobacterium Anabaena doliolum.
abstract: Effect of salt, copper, and temperature pretreatments on the UV-B-induced oxidative damage, measured in terms of peroxide and MDA (lipid peroxidation) contents, was studied in the diazotrophic cyanobacterium Anabaena doliolum. To understand the survival strategy enzymatic (superoxide dismutase, catalase, glutathione reductase, and ascorbate peroxidase) and non-enzymatic (glutathione, ascorbate, alpha-tocopherol and carotenoid) antioxidants were studied. Among the various pretreatments salt was found to decrease and copper and temperature pretreatments increased the deleterious effects of UV-B. This study is the first to demonstrate that physical stress (high temperature) enhanced the damaging effect of UV-B more profoundly than chemical stresses (salt and copper).
authors.affiliation: Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, India.
authors.forename: Ashish Kumar
authors.initials: AK
authors.lastname: Srivastava
authors.forename: Poonam
authors.initials: P
authors.lastname: Bhargava
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Mishra
authors.forename: Bideh
authors.initials: B
authors.lastname: Shukla
authors.forename: Lal Chand
authors.initials: LC
authors.lastname: Rai
mesh_terms: D017033:Anabaena / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000032:analysis; D002338:Carotenoids / Q000032:analysis; D002374:Catalase / Q000032:analysis; D003300:Copper / Q000494:pharmacology*; D005978:Glutathione / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D006358:Hot Temperature; D008054:Lipid Peroxides / Q000032:analysis; D010544:Peroxidases / Q000032:analysis; D010545:Peroxides / Q000032:analysis; D012492:Salts / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000032:analysis; D024502:alpha-Tocopherol / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D008054:Lipid Peroxides; D010545:Peroxides; D012492:Salts; D002338:Carotenoids; D003300:Copper; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D024502:alpha-Tocopherol; D001205:Ascorbic Acid