Influence of the cerebrospinal fluid laboratory parameters in the ELISA test for neurocysticercosis using a total cysticerci antigen.
To evaluate if the cerebrospinal fluid (CSF) parameters may influence the cysticercosis immunoreactivity response in the CSF. CSF samples of 109 patients were analyzed and classified in three groups, according to the neurological manifestations and the reactivity in antibody-enzyme linked immunosorbent assay (Ab-ELISA) testing in CSF for neurocysticercosis (NC): group A, 18 patients with neurological disorders compatible with NC and reactive Ab-ELISA in CSF for NC; group B, 50 patients with neurological disorders non-compatible with NC and reactive Ab-ELISA for NC; group C, 41 patients with neurological disorders non-compatible with NC and non-reactive Ab-ELISA in CSF for NC. The CSF analysis in group A was compatible with NC. The group B in comparison to the groups A and C presents higher frequency and intensity of hypercytosis, presence of red blood cells in CSF, protein concentration and immunological reactive test for other etiological agents (p<0.05). Based on the present data, we suggest that the inflammatory process and high protein concentration may determine false positive reactions in the Ab-ELISA test for NC in the CSF.
Casanova
Cristiane S
CS
Neurology Service, Gaffrèe Guinle University Hospital, Federal University of Rio de Janeiro State, Rio de Janeiro, RJ, Brazil.
Ribeiro
Maria José S P
MJ
Gonçalves
Reizer R
RR
Faria
Luiz Cláudio
LC
Peralta
José Mauro
JM
Puccioni-Sohler
Marzia
M
D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis; D003552:Cysticercus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D020019:Neurocysticercosis / Q000134:cerebrospinal fluid* / Q000276:immunology; D012372:ROC Curve; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
D000947:Antigens, Helminth; D002556:Cerebrospinal Fluid Proteins; D007074:Immunoglobulin G