title: Vacuolar protein sorting receptor in Schizosaccharomyces pombe.
abstract: The mechanism by which soluble proteins, such as carboxypeptidase Y, reach the vacuole in Saccharomyces cerevisiae is very similar to the mechanism of lysosomal protein sorting in mammalian cells. Vps10p is a receptor for transport of soluble vacuolar proteins in S. cerevisiae. vps10(+), a gene encoding a homologue of S. cerevisiae PEP1/VPS10, has been identified and deleted from the fission yeast Schizosaccharomyces pombe. Deletion of the vps10(+) gene resulted in missorting and secretion of Sch. pombe vacuolar carboxypeptidase Cpy1p, indicating that it is required for targeting Cpy1p to the vacuole. Sch. pombe Vps10p (SpVps10p) is a type I transmembrane protein and its C-terminal cytoplasmic tail domain is essential for Cpy1p transport to the vacuole. Cells expressing green fluorescent protein-tagged SpVps10p produced a punctate pattern of fluorescence, indicating that SpVps10p was largely localized in the Golgi compartment. In addition, Sch. pombe vps26(+), vps29(+) and vps35(+), encoding homologues of the S. cerevisiae retromer components VPS26, VPS29 and VPS35, were identified and deleted. Fluorescence microscopy demonstrated that SpVps10p mislocalized to the vacuolar membrane in these mutants. These results indicate that the vps26(+), vps29(+) and vps35(+) gene products are required for retrograde transport of SpVps10p from the prevacuolar compartment back to the Golgi in Sch. pombe cells.
authors.affiliation: Research Center, Asahi Glass Co. Ltd, Kanagawa, Yokohama 221-8755, Japan.|Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Iwaki
authors.affiliation: Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hosomi
authors.affiliation: Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Sanae
authors.initials: S
authors.lastname: Tokudomi
authors.affiliation: Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kusunoki
authors.affiliation: Research Center, Asahi Glass Co. Ltd, Kanagawa, Yokohama 221-8755, Japan.|Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Fujita
authors.affiliation: Research Center, Asahi Glass Co. Ltd, Kanagawa, Yokohama 221-8755, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Giga-Hama
authors.affiliation: Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Naotaka
authors.initials: N
authors.lastname: Tanaka
authors.affiliation: Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
mesh_terms: D019677:Artificial Gene Fusion; D002268:Carboxypeptidases / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D017930:Genes, Reporter; D006056:Golgi Apparatus / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; D002268:Carboxypeptidases