title: Use of semiconductor-based oligonucleotide microarrays for influenza a virus subtype identification and sequencing.
abstract: In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here we describe a genome assay and microarray design that can be used to rapidly identify influenza A virus hemagglutinin subtypes 1 through 15 and neuraminidase subtypes 1 through 9. Also described is an array-based enzymatic assay that can be used to sequence portions of both genes or any other sequence of interest.
authors.affiliation: CombiMatrix Corporation, 6500 Harbour Heights Parkway, Suite 301, Mukilteo, WA 98275, USA. mlodes@combimatrix.com
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Lodes
authors.forename: Dominic
authors.initials: D
authors.lastname: Suciu
authors.forename: Mark
authors.initials: M
authors.lastname: Elliott
authors.forename: Axel G
authors.initials: AG
authors.lastname: Stover
authors.forename: Marty
authors.initials: M
authors.lastname: Ross
authors.forename: Marcelo
authors.initials: M
authors.lastname: Caraballo
authors.forename: Kim
authors.initials: K
authors.lastname: Dix
authors.forename: James
authors.initials: J
authors.lastname: Crye
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Webby
authors.forename: Wanda J
authors.initials: WJ
authors.lastname: Lyon
authors.forename: David L
authors.initials: DL
authors.lastname: Danley
authors.forename: Andrew
authors.initials: A
authors.lastname: McShea
mesh_terms: D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D009980:Influenza A virus / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D009439:Neuraminidase / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012666:Semiconductors; D017422:Sequence Analysis, DNA
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase