[Studies on fingerprints of Bupleurum chinense from Hebei Province with HPLC-UV].
OBJECTIVE
To establish a HPLC fingerprint analysis method for identification of Bupleurum chinense of Hebei Province and compare the fingerprints of Radix Bupleuri collected from different habitats so as to establish a sensitive and specific method for controlling the quality of Radix Bupleuri.


METHODS
The HPLC-UV fingerprints of Radix Bupleuri from different habitats were obtained from Waters 1525 instruments. The HPLC separation was performed on a C18 analytical column gradient eluted with a mixture consisting of acetonitrile and water at a flow rate of 1.0 ml/min with UV detector at 203 nm. The temperature of column was 30 degrees C.


RESULTS
The mutual mode of HPLC-UV fingerprints was set up, and the similar degrees to the crude drugs of different habitats were compared.


CONCLUSIONS
It is simple and quick to differ Bupleurum chinense from different habitats with the method that can be used as a quality control item for Radix Bupleuri.
Xiao
Rong
R
Department of Pharmaceutical Analysis, School of Pharmacy of Hebei Medical University, Shijiazhuang 050017.
Wang
Chun-ying
CY
Zhang
Zhi-fei
ZF
Yuan
Zhi-fang
ZF
Zhang
Lan-tong
LT
D029972:Bupleurum / Q000737:chemistry* / Q000145:classification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D011208:Powders; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D