Nickel(II) affects poly(ADP-ribose) polymerase-mediated DNA repair in normal and cancer cells.
Nickel(II) can be genotoxic, but the mechanism of its genotoxicity is not fully understood and the process of DNA repair may be considered as its potential target. We studied the effect of nickel chloride on the poly(ADP-ribose) polymerase (PARP)-mediated repair of DNA damaged by gamma-radiation and idarubicin with the alkaline comet assay in normal and cancer cells. Our results indicate that nickel chloride at very low, non-cytotoxic concentration of 1 microM can affect PARP-mediated DNA repair of lesions evoked by idarubicin and gamma-radiation. We also suggest that in the quiescent lymphocytes treated with gamma-radiation, nickel(II) could interfere with DNA repair process independent of PARP.
Wozniak
Katarzyna
K
Department of Molecular Genetics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. wozniak@biol.uni.lodz.pl
Czechowska
Agnieszka
A
Blasiak
Janusz
J
D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D015255:Idarubicin / Q000494:pharmacology; D020014:K562 Cells; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D009532:Nickel / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
C022838:nickel chloride; D009532:Nickel; D011065:Poly(ADP-ribose) Polymerases; D015255:Idarubicin