Genotyping of Ureaplasma diversum isolates using pulsed-field electrophoresis.
Ureaplasma diversum has been associated with reproductive disorders in cattle and in the present study genotypic variations among U. diversum isolates obtained from the vaginal mucus of healthy cattle and sick animals were analyzed by enzymatic digestion and pulsed-field gel electrophoresis (PFGE). The influence of time and broth volume was important in obtaining sufficient cell sediment and DNA for PFGE. The method presented a high discriminatory power and satisfactory reproducibility for the analysis of detected variations among U. diversum isolates and strains. Different band profiles and wide genotypic heterogeneity were detected but no association between DNA polymorphism and sick or healthy animals could be established.
Buzinhani
Melissa
M
Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de São Paulo, Rua Professor Lineu Prestes, 1374 São Paulo, Brazil.
Buim
Marcos R
MR
Yamaguti
Maurício
M
Oliveira
Rosângela C
RC
Mettifogo
Elena
E
Timenetsky
Jorge
J
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005260:Female; D005838:Genotype; D011110:Polymorphism, Genetic; D014509:Ureaplasma / Q000145:classification / Q000235:genetics*; D016869:Ureaplasma Infections / Q000382:microbiology / Q000662:veterinary*
D004269:DNA, Bacterial