Determination of zonisamide by a coated monolithic column.
In this paper, a monolithic ODS-silica gel column dynamically coated with cetylpyridinium chloride (CPC) was used to demonstrate the high-speed and efficient separation of zonisamide (1,2-benzisoxazole-3-methanesulfonamide, ZNS), its raw material (1,2-benzisoxazole-3-methanecarbonic acid) and intermediate (sodium 1,2-benzisoxazole-3-methanesulfonate) in drugs. Using a 40 mmol/L sodium perchlorate solution (pH 7.0) containing 10% acetonitrile as eluent, the analytes were eluted with a sharp and symmetrical peak within 3.0 min, detected with UV detection at 210 nm. The column demonstrates excellent stability over time, and exhibits unusual selectivity for pharmaceutical analysis. Thus, by this developed method, zonisamide in drug samples can be determined rapidly with high recoveries and good selectivity.
Li
Jing
J
Department of Chemistry, Xixi campus, Zhejiang University, Hangzhou 310028, China.
Wu
Genfu
G
Zhu
Yan
Y
D000927:Anticonvulsants / Q000032:analysis / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007555:Isoxazoles / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D000078305:Zonisamide
D000927:Anticonvulsants; D002412:Cations; D007555:Isoxazoles; D012997:Solvents; D013501:Surface-Active Agents; D000078305:Zonisamide; D012822:Silicon Dioxide