Is current therapy of malignant gliomas beneficial for patients? Proteomics evidence of shifts in glioma cells expression patterns under clinically relevant treatment conditions.
The most common human brain tumours - gliomas - have poor prognosis with and without treatment. The current therapy conditions act sub-lethally and cannot effectively suppress the proliferation of glioma cells. Here we show differential protein expression patterns in surviving human malignant U87-MG glioma cells under clinically relevant chemo/radiotherapy. In parallel experiments, the cells underwent either irradiation (2 Gy, 200 KV X-ray) or chemotreatment with 30 microg/mL of temozolomide in the cultivation medium or combined chemo/radiation treatment. The cell cultures were treated during 5 days from day 4 until day 9 of growth. Modulated expression patterns of vimentin and RhoA GTPase indicate a potentially increasing grade of malignancy in treated cell fractions correlating well with extremely aggressive tumour phenotypes observed clinically at recidivation of treated malignant gliomas.
Trog
Daniela
D
Department of Radiology, Division of Molecular/Experimental Radiology, Friedrich-Wilhelms-University of Bonn, Bonn, Germany.
Fountoulakis
Michael
M
Friedlein
Arno
A
Golubnitschaja
Olga
O
D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D003606:Dacarbazine / Q000031:analogs & derivatives / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism / Q000532:radiotherapy*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000070796:Perilipin-3; D011257:Pregnancy Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D000077204:Temozolomide; D033921:Vesicular Transport Proteins; D020742:rhoA GTP-Binding Protein / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D018906:Antineoplastic Agents, Alkylating; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C038196:PLIN3 protein, human; D000070796:Perilipin-3; D011257:Pregnancy Proteins; D033921:Vesicular Transport Proteins; D003606:Dacarbazine; D020742:rhoA GTP-Binding Protein; D000077204:Temozolomide