Analysis of ingested material and urine by GC-MS and 1H NMR spectroscopy: poisoning of an adult with adulterated soda.
The purpose of this work is to characterize chemical compounds added to an ingested soda by (1)H nuclear magnetic resonance ((1)H NMR) spectroscopy and by gas chromatography-mass spectrometry in the electron impact mode. A second point was to highlight possible metabolic disturbances by considering urinary profile. Without any pretreatment, dimethylphtalate, 2-butanone, and 2,2,4-trimethylpentanediol diisobutyrate were found in the adulterated soda. Quantitative analysis was performed by relative integration of peak areas. Huge quantities of 2,2,4-trimethylpentanediol diisobutyrate and dimethylphtalate were found in the oily layer. 2-Butanone, which is miscible in water, was found in the two phases as well as small quantities of dimethylphtalate. The urine sample was collected on hospital admission and was also analyzed by (1)H NMR spectroscopy. The major abnormal compound found was 1,2-propanediol. Other disturbances concerned endogenous metabolites such as 2-ketoglutaric acid, lactic acid, and betaine.
Cartigny
Bernard
B
Laboratoire de Biochimie et de Biologie Moléculaire, Hôpital Albert Calmette, CHRU Lille, 59045 Lille Cedex, France.
Azaroual
Nathalie
N
Bochu
Christophe
C
Vinner
Elisabeth
E
Imbenotte
Michel
M
Vermeersch
Gaston
G
Lhermitte
Michel
M
D002074:Butanones / Q000652:urine; D002253:Carbonated Beverages / Q000032:analysis*; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D005517:Foodborne Diseases / Q000175:diagnosis* / Q000652:urine; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008875:Middle Aged; D009795:Octanes / Q000652:urine; D010795:Phthalic Acids / Q000652:urine; D019946:Propylene Glycol / Q000652:urine; D017132:Workplace; D015262:Xenobiotics / Q000506:poisoning* / Q000652:urine
D002074:Butanones; D009795:Octanes; D010795:Phthalic Acids; D015262:Xenobiotics; C024629:dimethyl phthalate; D019946:Propylene Glycol; C005222:methylethyl ketone; C045798:2,2,4-trimethylpentane