Characterization of storage proteins in wild (Glycine soja) and cultivated (Glycine max) soybean seeds using proteomic analysis.
A combined proteomic approach was applied for the separation, identification, and comparison of two major storage proteins, beta-conglycinin and glycinin, in wild (Glycine soja) and cultivated (Glycine max) soybean seeds. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) with three different immobilized pH gradient (IPG) strips was an effective method to separate a large number of abundant and less-abundant storage proteins. Most of the subunits of beta-conglycinin were well-separated in the pH range 3.0-10.0, while acidic and basic glycinin polypeptides were well-separated in pH ranges 4.0-7.0 and 6.0-11.0, respectively. Although the overall distribution pattern of the protein spots was similar in both genotypes using pH 3.0-10.0, variations in number and intensity of protein spots were better resolved using a combination of pH 4.0-7.0 and pH 6.0-11.0. The total number of storage protein spots detected in wild and cultivated genotypes was approximately 44 and 34, respectively. This is the first study reporting the comparison of protein profiles of wild and cultivated genotypes of soybean seeds using proteomic tools.
Natarajan
Savithiry S
SS
Soybean Genomics and Improvement Laboratory, Agricultural Research Service, U.S. Department of Agriculture, PSI, Beltsville, Maryland 20705, USA. natarajs@ba.ars.usda.gov
Xu
Chenping
C
Bae
Hanhong
H
Caperna
Thomas J
TJ
Garrett
Wesley M
WM
D052179:Antigens, Plant; D015180:Electrophoresis, Gel, Two-Dimensional; D005916:Globulins / Q000032:analysis / Q000302:isolation & purification; D005998:Glycine / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010940:Plant Proteins / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics; D055314:Seed Storage Proteins; D012639:Seeds / Q000737:chemistry*; D030262:Soybean Proteins / Q000032:analysis / Q000302:isolation & purification; D013025:Glycine max / Q000737:chemistry*
D052179:Antigens, Plant; D005916:Globulins; D010940:Plant Proteins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C011474:glycinin; D005998:Glycine