title: Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato.
abstract: The aim of this work was isolation and purification of the major immunodominant protein, Outer surface protein C (OspC) of three members of the species group Borrelia burgdorferi, the causative agent of Lyme disease. Our aim was to obtain this protein in a quantity and purity sufficient for immunization of experimental animals. For optimalization of protein purification's yield we used immobilized metal ion affinity chromatography (IMAC) under different conditions. The greatest efficiency was achieved by using of HiTrap Chelating Column under native conditions.
authors.affiliation: Department of Immunology, Palacký University, Olomouc, Czech Republic. majkl.krupka@centrum.cz
authors.forename: Michal
authors.initials: M
authors.lastname: Krupka
authors.forename: Jana
authors.initials: J
authors.lastname: Bĕláková
authors.forename: Martina
authors.initials: M
authors.lastname: Sebestová
authors.forename: Jana
authors.initials: J
authors.lastname: Tuhácková
authors.forename: Milan
authors.initials: M
authors.lastname: Raska
authors.forename: Vladimír
authors.initials: V
authors.lastname: Vrzal
authors.forename: Evzen
authors.initials: E
authors.lastname: Weigl
mesh_terms: D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D004926:Escherichia coli; D005822:Genetic Vectors; D022123:Lyme Disease Vaccines / Q000096:biosynthesis* / Q000302:isolation & purification; D019444:Vaccines, DNA / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D022123:Lyme Disease Vaccines; C079616:OspC protein; D019444:Vaccines, DNA