Lignan compounds and 4,4'-dihydroxybiphenyl protect C2C12 cells against damage from oxidative stress.
Lignan compounds are known to have various biological activities, especially antioxidative effects. We investigated whether lignan compounds show antioxidative activity in myoblast C2C12 cells. Among 14 lignan compounds investigated, two lignans containing two phenolic functional groups, namely Gomisin J and GR-12, prevented hydrogen peroxide (H(2)O(2))-induced cell death. A simple compound, 4,4'-dihydroxybiphenyl, which was found to be a common component of Gomisin J and GR-12, also largely prevented H(2)O(2)-induced cell death and almost completely prevented H(2)O(2)-induced increases in p38 MAPK phosphorylation. Our present results provide a useful in vitro system for clarifying the molecular mechanisms of lignan-mediated antioxidative effects and evaluating lead molecules toward the development of therapeutic drugs.
Yoshikawa
Ayumu
A
Department of Pharmacology, Saitama Medical School of Medicine, 38 Moro-Hongo, Moroyama-cho, Saitama 350-0492, Japan. yoshi3@saitama-med.ac.jp
Saito
Yumiko
Y
Maruyama
Kei
K
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002478:Cells, Cultured; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D017705:Lignans / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D032446:Myoblasts / Q000187:drug effects / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects*; D011083:Polycyclic Compounds / Q000737:chemistry / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors
D000975:Antioxidants; D001713:Biphenyl Compounds; C511184:GR-12 compound; D017705:Lignans; D011083:Polycyclic Compounds; D006861:Hydrogen Peroxide; D048051:p38 Mitogen-Activated Protein Kinases; C027439:4,4'-dihydroxybiphenyl; C082091:gomisin J