G-specific RNA-cleaving conjugates of short peptides and oligodeoxyribonucleotides.
Artificial ribonucleases, conjugates of short oligodeoxyribonucleotides and peptides built of arginine, leucine, proline, and serine, were synthesized and assessed in terms of ribonuclease activity and specificity of RNA cleavage. A specific group of the conjugates was identified that display T1-ribonuclease-like activity and cleave RNA predominantly at G-X sequences. Circular dichroism study of the structures of the most active conjugates, free peptide (LR)4G, and oligonucleotides revealed that conjugation of oligonucleotide to the peptide results in a specific peptide folding that possibly provides ribonuclease activity to the conjugate.
Mironova
Nadezhda L
NL
Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Russian Federation, Novosibirsk, 630090.
Pyshnyi
Dmitrii V
DV
Stadler
Dmitry V
DV
Prokudin
Ivan V
IV
Boutorine
Yuri I
YI
Ivanova
Eugenia M
EM
Zenkova
Marina A
MA
Gross
Hans J
HJ
Vlassov
Valentin V
VV
D020029:Base Pairing; D002942:Circular Dichroism; D006147:Guanine / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D009842:Oligopeptides; D006147:Guanine; D012313:RNA; D012260:Ribonucleases