title: Ovicidal and larvicidal activity of Melia azedarach extracts on Haemonchus contortus.
abstract: Haemonchus contortus is responsible for severe economic losses in sheep and goat breeding in the Northeast of Brazil. However, the effectiveness of control is compromised due to anthelmintic resistance and misuse. In the search for natural anthelmintics, Melia azedarach L., a plant indigenous to India but now distributed throughout Brazil, was selected due to the reported anthelmintic properties of its seeds. The aim of this study was to evaluate the anthelmintic activity of the seed and leaf extracts of the Brazilian adapted plant and investigate the type of organic chemical compounds present in the most active extracts. The ovicidal and larvicidal activity of M. azedarach extracts on H. contortus was evaluated through egg hatching and larval development tests. Hexane and ethanol extracts of seeds and chloroform and ethanol extracts of leaves of M. azedarach were used in the tests. To perform the larval development test, feces of an animal free from parasites were mixed with third instar H. contortus larvae and extracts in several concentrations. The coprocultures were incubated for 7 days at 30 degrees C, then the larvae were recovered and counted. LC50 was calculated by probits using the SPSS 8.0 program. The seed ethanol extract was the most active on eggs (LC50=0.36mgmL(-1)) and the leaf ethanol extract showed the best inhibition of larval development (LC50=9.18mgmL(-1)). Phytochemical analysis of the most active extracts revealed the presence of condensed tannins, triterpenes and alkaloids.
authors.affiliation: College of Veterinary Medicine, Ceará State University, Av. Paranjana 1700, Campus do Itaperi, CEP 60740-000, Fortaleza, Ceará, Brazil.
authors.forename: M V
authors.initials: MV
authors.lastname: Maciel
authors.forename: S M
authors.initials: SM
authors.lastname: Morais
authors.forename: C M L
authors.initials: CM
authors.lastname: Bevilaqua
authors.forename: A L F
authors.initials: AL
authors.lastname: Camurça-Vasconcelos
authors.forename: C T C
authors.initials: CT
authors.lastname: Costa
authors.forename: C M S
authors.initials: CM
authors.lastname: Castro
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005243:Feces / Q000469:parasitology; D015511:Goat Diseases / Q000188:drug therapy / Q000469:parasitology; D006041:Goats; D006188:Haemonchiasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects* / Q000254:growth & development; D066298:In Vitro Techniques; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D008018:Life Cycle Stages / Q000187:drug effects; D031586:Melia azedarach / Q000737:chemistry*; D010063:Ovum / Q000187:drug effects; D010572:Pest Control, Biological; D008517:Phytotherapy / Q000662:veterinary*; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy / Q000469:parasitology; D016896:Treatment Outcome
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts