Newcastle disease virus (strain Herts 33/56) in tissues and organs of chickens infected experimentally.
Six-week-old susceptible specific pathogen free chickens were infected intranasally with the virulent Newcastle disease virus strain Herts 33/56 and the levels of virus present in blood, faeces, breast muscle, leg muscle and a pool of heart/kidney/spleen were estimated in birds killed humanely at each day post inoculation. Highest titres were recorded at day 4 post inoculation when titres of virus were 10(6) median egg infectious doses (EID50)/g in the heart/kidney/spleen pool, 10(4.2) EID50/g in the leg muscle and 10(4) EID50/g in the breast muscle and faeces. A median oral infectious dose of Newcastle disease virus strain Herts 33/56 for 3-week-old chickens was estimated to be equivalent to 10(4) EID50.
Alexander
Dennis J
DJ
Virology Department, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey, KT15 3NB, UK. d.j.alexander@vla.defra.gsi.gov.uk
Manvell
Ruth J
RJ
Parsons
Graham
G
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D005243:Feces / Q000821:virology; D006321:Heart / Q000821:virology; D007668:Kidney / Q000821:virology; D018482:Muscle, Skeletal / Q000821:virology; D009521:Newcastle Disease / Q000097:blood / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000821:virology