title: Reliable amplification method for bacterial RNA.
abstract: DNA microarray technology has been increasingly applied for studies of clinical samples. Frequently, RNA probes from clinical samples are available in limited amounts. We describe a reliable amplification method for bacterial RNA. We verified this method on mycobacterial RNA applying mycobacterial genome-directed primers (mtGDPs). Glass slide-based oligoarrays were employed to assess the quality of the amplification method. We observed a relatively small bias in amplified RNA pool when compared to the unamplified one. Up to 1000-fold linear RNA amplification in a single amplification round was obtained. To our knowledge, this study describes the first amplification method for mycobacterial RNA.
authors.affiliation: Max Planck Institute for Infection Biology, Department of Immunology, Schumannstrasse 21-22, 10117 Berlin, Germany.
authors.forename: Helmy
authors.initials: H
authors.lastname: Rachman
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Joerg
authors.initials: J
authors.lastname: Angermann
authors.forename: Jane
authors.initials: J
authors.lastname: Kowall
authors.forename: Stefan H E
authors.initials: SH
authors.lastname: Kaufmann
mesh_terms: D008979:Moloney murine leukemia virus / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000494:pharmacology
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012194:RNA-Directed DNA Polymerase