title: Determination of major carotenoids in vegetables by capillary electrochromatography.
abstract: A simple and rapid method for the isocratic separation and determination of carotenoids (carotenes and xanthophylls) in vegetables by CEC is described. The capillary column (100 microm ID, 25 cm effective length) was packed with 3 microm Hypersil ODS particles. The optimized mobile phase contained 60% ACN, 35% THF and 5% of a 5 mM Tris aqueous buffer of pH 8. beta-Carotene, lycopene and lutein were separated with efficiencies of 66 000-128 000 plates/m within a short time (less than 12 min for the last peak eluted, 13/13'-cis-beta-carotene). An excellent resolution of the three carotenoids, as well as partial resolution of their geometrical isomers, was achieved. Application to the determination of the analytes in carrot, tomato, spinach and corn was demonstrated.
authors.affiliation: Department of Analytical Chemistry, University of Valencia, Burjassot, Spain. jmherrer@uv.es
authors.forename: José M
authors.initials: JM
authors.lastname: Herrero-Martínez
authors.forename: Sebastiaan
authors.initials: S
authors.lastname: Eeltink
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Schoenmakers
authors.forename: Wim Th
authors.initials: WT
authors.lastname: Kok
authors.forename: Guillermo
authors.initials: G
authors.lastname: Ramis-Ramos
mesh_terms: D002338:Carotenoids / Q000032:analysis / Q000302:isolation & purification; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D013237:Stereoisomerism; D014675:Vegetables / Q000737:chemistry*
chemical_list: D002338:Carotenoids