Single nucleotide polymorphisms for integrative mapping in the Turkey (Meleagris gallopavo).
When multiple genetic maps exist for a species, integration of these maps requires a set of common markers be genotyped across the individual mapping populations. In the turkey, three genetic maps based on separate mapping populations are available. In this study, SNP-based markers were developed for integrating the cDNA/RFLP-based map (1) with microsatellite markers of the second-generation turkey genome map (2). Forty-eight primer sets were designed and tested and 33 (69%) correctly amplified turkey genomic DNA by PCR. Putative SNPs were detected in 20 (61%) of the amplified gene fragments, and 10 SNP markers were subsequently genotyped by PCR/RFLP for segregation analysis. Eight SNP markers were incorporated into the turkey genetic map.
Reed
K M
KM
Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, Minnesota 55108, USA. reedx054@umn.edu
Hall
M K
MK
Chaves
L D
LD
Knutson
T P
TP
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods / Q000662:veterinary*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers; D020224:Expressed Sequence Tags; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D014422:Turkeys / Q000235:genetics*
D017931:DNA Primers; D004247:DNA