title: Microrheological characterization of anisotropic materials.
abstract: We describe the measurement of anisotropic viscoelastic moduli in complex soft materials, such as biopolymer gels, via video particle tracking microrheology of colloid tracer particles. The use of a correlation tensor to find the axes of maximum anisotropy without prior knowledge, and hence the mechanical director, is described. The moduli of an aligned DNA gel are reported, as an application of the technique; this may have implications for high DNA concentrations in vivo. We also discuss the errors in microrheological measurement, and describe the use of frequency space filtering to improve displacement resolution, and hence probe these typically high modulus materials.
authors.affiliation: Biological and Soft Systems, Cavendish Laboratory, University of Cambridge, Madingley Road, Cambridge CB3 0HE, UK.
authors.forename: I A
authors.initials: IA
authors.lastname: Hasnain
authors.forename: A M
authors.initials: AM
authors.lastname: Donald
mesh_terms: D016880:Anisotropy; D001672:Biocompatible Materials / Q000737:chemistry*; D003198:Computer Simulation; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004548:Elasticity; D005782:Gels / Q000032:analysis / Q000737:chemistry*; D044085:Microfluidics / Q000379:methods*; D008956:Models, Chemical; D033081:Shear Strength; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D004247:DNA