title: Interaction between Snapin and G-CSF receptor.
abstract: Granulocyte colony-stimulating factor (G-CSF) is the major cytokine involved in the control of neutrophil development. G-CSF activates the special receptor, the G-CSF receptor (GCSF-R), which subsequently triggers multiple signaling events. To obtain more interactive molecules with GCSF-R and to further understand the cellular signaling mechanism of GCSF-R, yeast two-hybrid system was used to screen a mouse liver library. Here, the interaction of GCSF-R and Snapin was found by yeast two-hybrid experiment, and the interaction of the two proteins was further confirmed by GST pull-down experiment, mammalian two-hybrid experiment and co-immunoprecipitation study. Moreover, the immuno-fluorescence assay was shown that the two proteins of GCSF-R with Snapin were co-localized in the cytoplasm and plasma membrane. The region of C-terminal GCSF-R between box2 and box3, including the residue Tyr703, was responsible for the interaction with Snapin. These data suggested that Snapin is a new interactive protein of GCSF-R.
authors.affiliation: Department of Pathophysiology, Beijing Institute of Radiation Medicine, No. 27 Taiping Road, Beijing 100850, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Yuan
authors.forename: Yajun
authors.initials: Y
authors.lastname: Shan
authors.forename: Zhenhu
authors.initials: Z
authors.lastname: Zhao
authors.forename: Jiapei
authors.initials: J
authors.lastname: Chen
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Cong
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D051379:Mice; D011485:Protein Binding; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011993:Recombinant Fusion Proteins; C498451:Snapin protein, mouse; D033921:Vesicular Transport Proteins