[Detection of mdr1 gene by real-time fluorescence quantitative polymerase chain reaction using Taq Man-MGB probe].
Primer Express 2.0 software was used to design the primers and the MGB probe. With the plasmid pHaMDR1/A containing mdr1 cDNA as the template, we established a real-time fluorescent quantitative polymerase chain reaction system, which, at the template concentration of 3.061 x 10(3) to 3.061 x 10(9) cps/ml, had a correlation coefficient of 0.988243 between template concentration and threshold cycle value. This PCR method allows sensitive, specific and quantitative detection of human mdr1 gene.
Zou
Ya-wei
YW
Department of Pediatrics, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China. zouyawei@163.com
Feng
Zhi-chun
ZC
Hu
Bin
B
Qiao
Ying-sa
YS
Wu
Zi-liang
ZL
Chen
Fu-xiong
FX
Ye
Tie-zhen
TZ
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis* / Q000235:genetics; D017931:DNA Primers; D005260:Female; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods; D019450:Genes, MDR / Q000235:genetics*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D017931:DNA Primers; D005456:Fluorescent Dyes; D019914:Taq Polymerase