A novel N-chlorotaurine-corticosteroid combination as a preservative-free local disinfectant: influence on the ciliary beat frequency in vitro.
CONCLUSIONS
The combination of N-chlorotaurine (NCT) and a corticosteroid seems to be a very promising substance for the local therapy of ENT infections. As it can be used without any preservatives, the effect on the ciliary beat frequency (CBF) is much less than that of products containing benzalkonium chloride (BAC). The in vitro results obtained in this study encourage us to perform clinical trials on this novel combination for intranasal application.


OBJECTIVE
To investigate the influence of a novel mixture of NCT and a corticosteroid [fluticasone propionate (FP)] on the CBF of human ciliated cells in vitro.


METHODS
The study was designed as an in vitro study. CBF was measured by means of a photometric technique involving the combination of a light microscope, a photometer, a photographic multiplier and a computerized analyzing unit.


RESULTS
The combination of 1% NCT + 0.5 mg/ml FP decreased the CBF to 42.17% of its original value after 20 min. Treatment with BAC lowered the CBF depending on the concentration to 96.61% of its original value with 0.04 mg/ml, to 91.90% with 0.1 mg/ml, to 63.46% with 0.2 mg/ml and to 0% with 0.5 mg/ml. After rinsing in saline, the CBF of samples treated with 1% NCT + 0.5 mg/ml FP recovered to 68.93% of its original value.
Arnitz
Roland
R
Department of Otorhinolaryngology, Innsbruck Medical University, Innsbruck, Austria.
Ott
Helmut Werner
HW
Gstöttner
Michaela
M
Nagl
Markus
M
Scholtz
Arne W
AW
Neher
Andreas
A
D000730:Androstadienes / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002478:Cells, Cultured; D002923:Cilia / Q000187:drug effects; D004202:Disinfectants; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D000068298:Fluticasone; D006801:Humans; D066298:In Vitro Techniques; D020714:Maximum Tolerated Dose; D009079:Mucociliary Clearance / Q000187:drug effects*; D009297:Nasal Mucosa / Q000166:cytology*; D012680:Sensitivity and Specificity; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
D000730:Androstadienes; D000891:Anti-Infective Agents, Local; D004202:Disinfectants; D004338:Drug Combinations; D013654:Taurine; C043410:N-chlorotaurine; D000068298:Fluticasone