title: Genotoxic and stress inductive potential of cadmium in Xenopus laevis larvae.
abstract: The present investigation evaluates the toxic potential of Cd in larvae of the frog Xenopus laevis after 12 days of exposure to environmentally relevant contamination levels, close to those measured in the river Lot (France). Several genotoxic and detoxification mechanisms were analyzed in the larvae: clastogenic and/or aneugenic effects in the circulating blood by micronucleus (MN) induction, metallothionein (MT) production in whole larvae, gene analyses and Cd content in the liver and also in the whole larvae. The results show: (i) micronucleus induction at environmental levels of Cd contamination (2, 10, 30 microgL(-1)); (ii) an increased and concentration-dependent quantity of MT in the whole organism after contamination with 10 and 30 microgCdL(-1) (a three- and six-fold increase, respectively) although no significant difference was observed after contamination with 2 microgCdL(-1); (iii) Cd uptake by the whole organism and by the liver as a response to Cd exposure conditions; (4) up-regulation of the genes involved in detoxification processes and response to oxidative stress, while genes involved in DNA repair and apoptosis were repressed. The results confirm the relevance of the amphibian model and highlight the complementarity between a marker of genotoxicity, MT production, bioaccumulation and genetic analysis in the evaluation of the ecotoxicological impact.
authors.affiliation: Laboratoire d'Ecologie des Hydrosystèmes LEH, UMR CNRS UPS 5177, Université Paul Sabatier, 29 Rue Jeanne Marvig, 31400 Toulouse, France. mouchet@cict.fr
authors.forename: F
authors.initials: F
authors.lastname: Mouchet
authors.forename: M
authors.initials: M
authors.lastname: Baudrimont
authors.forename: P
authors.initials: P
authors.lastname: Gonzalez
authors.forename: Y
authors.initials: Y
authors.lastname: Cuenot
authors.forename: J P
authors.initials: JP
authors.lastname: Bourdineaud
authors.forename: A
authors.initials: A
authors.lastname: Boudou
authors.forename: L
authors.initials: L
authors.lastname: Gauthier
mesh_terms: D000818:Animals; D002104:Cadmium / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D017931:DNA Primers / Q000737:chemistry; D004781:Environmental Exposure; D015870:Gene Expression / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007814:Larva / Q000187:drug effects / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000032:analysis; D015162:Micronucleus Tests / Q000662:veterinary; D009152:Mutagenicity Tests / Q000662:veterinary; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D015854:Up-Regulation; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D014982:Xenopus laevis / Q000235:genetics / Q000502:physiology*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D002104:Cadmium; D008668:Metallothionein