[A comparative study on the resistance of bacteriophage T4, phiXM174D, and f2 to glutaraldehyde in laboratory].
OBJECTIVE
To identify the best indicative bacteriophage in disinfection tests through comparing the resistance of bacteriophage T4, phiX174D, and f2 to glutaraldehyde.


METHODS
The virucidal activities of glutaraldehyde against bacteriophage T4, phiX174D, and f2 were assessed with suspension tests along with neutralizer tests. The double-agar-layer plaque technique was used to detect the bacteriophage T4, phiX174D, and f2.


RESULTS
(1)In a condition of 3000 mg/L of glutaraldehyde and 20 minutes of contact or 6000 mg/L of glutaraldehyde and 5 minutes of contact, "disinfection" level for bacteriophage T4 was achieved, with the log10 inactivation value (LIV) or log10o reduction value (LRV) (=log10No-log10Nt) > or = 4. 00 log10. (2) In a condition of 2500 mg/L of glutaraldehyde and 20 minutes of contact or 5000 mg/L of glutaraldehyde and 5 minutes of contact, the LIV for bacteriophage phiX174D reached "disinfection" level; (3) In a condition of 4000 mg/L of glutaraldehyde and 40 minutes of contact or 8000 mg/L of glutaraldehyde and 10 minutes of contact, the LIV for bacteriophage f2 reached "disinfection" level.


CONCLUSIONS
Bacteriophage f2 and bacteriophage phiX174D has the strongest and weakest resistance to glutaraldehyde respectively.
Chen
Zhao-Bin
ZB
Department of Medical Laboratory Technology, West China School of Public health, Sichuan University, Chengdu 610041, China.
Zhang
Chao-Wu
CW
Xu
Xin
X
Zhu
Min
M
Wang
Guo-Qing
GQ
D017122:Bacteriophage T4 / Q000187:drug effects*; D010584:Bacteriophage phi X 174 / Q000187:drug effects*; D001435:Bacteriophages / Q000187:drug effects; D004203:Disinfection; D024882:Drug Resistance, Viral; D005976:Glutaral / Q000494:pharmacology*
D005976:Glutaral