title: Rapid detection and identification of Mycobacterium tuberculosis by Real Time PCR and Bactec 960 MIGT.
abstract: We have developed a Real-Time PCR assay to detect M. tuberculosis using the iCycler iQ detection system by TaqMan assay directly on the clinical specimen. A total of 513 clinical samples were taken from patients with suspected tuberculosis and other patients that had an active mycobacterial infection, as well as patients with diagnosed tuberculosis who were receiving antitubercular therapy. The sensitivity and specificity of this assay, 10% and 100%, respectively, were compared to those of conventional microbiological methods.
authors.affiliation: Dip. di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli Studi di Sassari, Italy. silviaortu@vahoo.it
authors.forename: Silvia
authors.initials: S
authors.lastname: Ortu
authors.forename: Paola
authors.initials: P
authors.lastname: Molicotti
authors.forename: Leonardo Antonio
authors.initials: LA
authors.lastname: Sechi
authors.forename: Pierp
authors.initials: P
authors.lastname: Pirina
authors.forename: Franca
authors.initials: F
authors.lastname: Saba
authors.forename: Cono
authors.initials: C
authors.lastname: Vertuccio
authors.forename: Antonella
authors.initials: A
authors.lastname: Deriu
authors.forename: Ivana
authors.initials: I
authors.lastname: Maida
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Mura
authors.forename: Stefania
authors.initials: S
authors.lastname: Zanetti
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000737:chemistry; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D019914:Taq Polymerase