title: [Expression of human LNalpha4LG4-5 in Pichia yeast].
abstract: To carry out the secretive expression of human laminin alpha4 chain LG4-5 module (hLNalpha4LG4-5), recombinant expression plasmid pPICZalphaA-LG45 was constructed by inserting of hLNalpha4LG4-5 cDNA into yeast expression vector pPICZaA. The hLNalpha4LG4-5 protein was expressed in GS115 Pichia yeast strain after induced by methanol, and purified target protein can obviously promote the expansion and adhesion of 293 cells.
authors.affiliation: Faculty of Basic Medical Science, Third Military Medical University, Chongqing 400038, China.
authors.forename: Ji-Qin
authors.initials: JQ
authors.lastname: Lian
authors.forename: Xu-Fang
authors.initials: XF
authors.lastname: Dai
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Li
authors.forename: Feng-Tian
authors.initials: FT
authors.lastname: He
mesh_terms: D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D007797:Laminin / Q000096:biosynthesis* / Q000235:genetics; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*
chemical_list: C501369:LAMA4 protein, human; D007797:Laminin; D011993:Recombinant Fusion Proteins