title: Differential abilities of mouse liver parenchymal and nonparenchymal cells in HDL and LDL (native and oxidized) association and cholesterol efflux.
abstract: The aim of this study was to quantify the abilities of mouse liver parenchymal and nonparenchymal cells with respect to (i) cholesteryl ester (CE) selective uptake from low-density lipoproteins (LDL), oxidized LDL (OxLDL), and high-density lipoprotein (HDL); and (ii) their free cholesterol efflux to HDL. The preparations of cells were incubated with lipoproteins labelled either in protein with iodine-125 or in CE with 3H-cholesterol oleate, and lipoprotein-protein and lipoprotein-CE associations were measured. The associations of LDL-protein and LDL-CE with nonparenchymal cells were 5- and 2-fold greater, respectively, than with parenchymal cells. However, in terms of CE-selective uptake (CE association minus protein association) both types of cell were equivalent. Similar results were obtained with OxLDL, but both types of cell showed higher abilities in OxLDL-CE than in LDL-CE selective uptake (on average by 3.4-fold). The association of HDL-protein with nonparenchymal cells was 3x that with parenchymal cells; however, nonparenchymal cells associated 45% less HDL-CE. Contrary to parenchymal cells, nonparenchymal cells did not show HDL-CE selective uptake activity. Thus parenchymal cells selectively take CE from the 3 types of lipoproteins, whereas nonparenchymal cells exert this function only on LDL and OxLDL. Efflux was 3.5-fold more important in nonparenchymal than in parenchymal cells.
authors.affiliation: Département des Sciences Biologiques, Université du Québec à Montréal, Canada.
authors.forename: Jany
authors.initials: J
authors.lastname: Lapointe
authors.forename: To Quyen
authors.initials: TQ
authors.lastname: Truong
authors.forename: Louise
authors.initials: L
authors.lastname: Falstrault
authors.forename: Louise
authors.initials: L
authors.lastname: Brissette
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002469:Cell Separation; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D005434:Flow Cytometry; D022781:Hepatocytes / Q000145:classification / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction
chemical_list: D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D002784:Cholesterol