[Production and identification of monoclonal antibody against N terminal of AIB1].
OBJECTIVE
To make and identify the monoclonal antibody against AIB1-N.


METHODS
BALB/c mice were immunized with purified GST-AIB1-N protein, McAb against AIB1-N was produced by hybridoma technique. ELISA and Western-blot were used to identify the immunoglobin subtype and specificity. Results A hybridoma cell was successfully produced to secrete the McAb against AIB1-N, which was identified to belong in IgG1 subtype. By western-blot, the McAb against AIB1 displayed strongly specificity and high affinity.


CONCLUSIONS
The McAb against AIB1 protein may be a useful tool for studying the biological properties of AIB1 expression and the clinical laboratory detection.
Pang
Qiu-Xia
QX
West China Second Hospital, Sichuan University, Chengdu 610041, China.
Peng
Xiao-Dong
XD
Xi
Ming-Rong
MR
Zhu
Yue-Ming
YM
Peng
Xu-Hong
XH
Dong
Wei
W
Zhang
Lin
L
Qu
Yi
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000912:Antibodies, Neoplasm / Q000096:biosynthesis / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology*; D005260:Female; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D056921:Nuclear Receptor Coactivator 3; D014157:Transcription Factors / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D014157:Transcription Factors; D056921:Nuclear Receptor Coactivator 3