title: Production of butter fat rich in trans10-C18:1 for use in biomedical studies in rodents.
abstract: Trans fatty acids are suspected to be detrimental to health, particularly to cardiovascular function. Trans fatty acids include a wide range of fatty acids, with isomers of C18:1, conjugated and non-conjugated C18:2 as major components. A vaccenic acid (trans11-C18:1) + rumenic acid (cis9,trans11-CLA)-rich butter has been shown previously to exhibit health beneficial effects, but less is known concerning another trans-C18:1 present in hydrogenated vegetable oil-based products and sometimes in milk fat, the trans10-isomer. The present experiment was conducted to produce butters from milk of variable fatty acid composition for use in biomedical studies with rodents, with the overall aim of evaluating the specific effect of trans10-C18:1 and trans11-C18:1 + cis9,trans11-CLA on cardiovascular function. Milks from lactating dairy cows fed two types of maize-based diets supplemented (5% of dry matter)--or not--with sunflower oil were collected, and used to manufacture butters either rich in trans10-C18:1 (14% of total fatty acids, 64.5% of fat content) or rich in trans11-C18:1 + cis9,trans11-CLA (7.4 and 3.1% of total fatty acids, respectively, 68.5% of fat content), or with standard fatty acid composition (70% of fat content). Additionally, total saturated fatty acid percentage was reduced by more than one third in the enriched butters compared with the standard butter. An understanding of the role of nutrition on milk fatty acid composition in cows allows for the production of dairy products of variable lipid content and composition for use in biomedical studies in animal models and human subjects.
authors.affiliation: Herbivore Research Unit INRA-Theix, 63122 St-Genès-Champanelle, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Roy
authors.forename: Anne
authors.initials: A
authors.lastname: Ferlay
authors.forename: Yves
authors.initials: Y
authors.lastname: Chilliard
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D050197:Atherosclerosis / Q000178:diet therapy; D002079:Butter / Q000032:analysis; D002320:Cardiovascular Physiological Phenomena / Q000187:drug effects*; D002417:Cattle / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D007536:Isomerism; D007774:Lactation / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000032:analysis / Q000378:metabolism; D008892:Milk / Q000737:chemistry*; D010938:Plant Oils / Q000008:administration & dosage* / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D012377:Rodentia; D000074242:Sunflower Oil; D044242:Trans Fatty Acids / Q000032:analysis* / Q000378:metabolism
chemical_list: D044243:Linoleic Acids, Conjugated; D010938:Plant Oils; D000074242:Sunflower Oil; D044242:Trans Fatty Acids; D002079:Butter