Hemangioblastomas share protein expression with embryonal hemangioblast progenitor cell.
Hemangioblastomas are central nervous system (CNS) tumors of unknown histogenesis, which can occur sporadically or in von Hippel-Lindau disease. Hemangioblastomas are composed of neoplastic "stromal" cells of unknown origin, accompanied by intensive reactive angiogenesis. Failure to specify the cytologic origin of the stromal cell has precluded the development of nonsurgical therapies and limits understanding of its basic biology. We report that the stromal cells express proteins (Scl, brachyury, Csf-1R, Gata-1, Flk-1, and Tie-2) that characterize embryonic progenitor cells with hemangioblastic differentiation potential and conclude that embryonic progenitors with hemangioblast potential represent a possible cytologic equivalent of the stromal cell. We also identified a new autocrine/paracrine stimulatory loop between the receptor Tie-2 and the hypoxia-inducible factor target Ang-1, which, combined with previous observations, suggests that a variety of autocrine loops may be initiated in hemangioblastomas, depending on the differentiation status of the tumor cells and the extent of HIF downstream activation. Finally, the consistent identification of Scl in the stromal cells may help explain the unique and characteristic topographical distribution of hemangioblastomas within the CNS.
Gläsker
Sven
S
Surgical Neurology Branch, National Institutes of Neurological Disorders and Stroke, NIH, Bethesda, Maryland 20892, USA.
Li
Jie
J
Xia
John B
JB
Okamoto
Hiroaki
H
Zeng
Weifen
W
Lonser
Russell R
RR
Zhuang
Zhengping
Z
Oldfield
Edward H
EH
Vortmeyer
Alexander O
AO
D042683:Angiopoietin-1 / Q000096:biosynthesis; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis; D002528:Cerebellar Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D005326:Fetal Proteins / Q000096:biosynthesis; D050982:GATA1 Transcription Factor / Q000096:biosynthesis; D018325:Hemangioblastoma / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D014176:Protein Biosynthesis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D042787:Receptor, TIE-2 / Q000096:biosynthesis; D017154:Stromal Cells / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000096:biosynthesis; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis; D000098908:Brachyury Protein
D042683:Angiopoietin-1; D051792:Basic Helix-Loop-Helix Proteins; D005326:Fetal Proteins; D050982:GATA1 Transcription Factor; D011518:Proto-Oncogene Proteins; D016186:Receptor, Macrophage Colony-Stimulating Factor; D042787:Receptor, TIE-2; D020825:T-Box Domain Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D040301:Vascular Endothelial Growth Factor Receptor-2; D000098908:Brachyury Protein; C494688:GATA1 protein, human; C067143:TAL1 protein, human