title: Enzymatic synthesis of prebiotic oligosaccharides.
abstract: Prebiotic oligosaccharides are nondigestible carbohydrates that can be obtained by enzymatic synthesis. Glucosyltransferases can be used to produce these carbohydrates through an acceptor reaction synthesis. When maltose is the acceptor a trisaccharide composed of one maltose unit and one glucose unit linked by an alpha-1,6-glycosidic bond (panose) is obtained as the primer product of the dextransucrase acceptor reaction. In this work, panose enzymatic synthesis was evaluated by a central composite experimental design in which maltose and sucrose concentration were varied in a wide range of maltose/sucrose ratios in a batch reactor system. A partially purified enzyme was used in order to reduce the process costs, because enzyme purification is one of the most expensive steps in enzymatic synthesis. Even using high maltose/sucrose ratios, dextran and higher-oligosaccharide formation were not avoided. The results showed that intermediate concentrations of sucrose and high maltose concentration resulted in high panose productivity with low dextran and higher-oligosaccharide productivity.
authors.affiliation: Departamento de Tecnologia de Alimentos, Universidade Federal do Ceara, Av. Mister Hull, 2977, bloco 858, Campus do Pici, CEP 60356-000 Fortaleza, CE, Brazil.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Rabelo
authors.forename: Talita L
authors.initials: TL
authors.lastname: Honorato
authors.forename: Luciana R B
authors.initials: LR
authors.lastname: Gonçalves
authors.forename: Gustavo A S
authors.initials: GA
authors.lastname: Pinto
authors.forename: Sueli
authors.initials: S
authors.lastname: Rodrigues
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry; D016695:Glycosyltransferases / Q000737:chemistry*; D007934:Leuconostoc / Q000201:enzymology*; D008320:Maltose; D009844:Oligosaccharides / Q000138:chemical synthesis*; D013395:Sucrose / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D005936:Glucans; D009844:Oligosaccharides; C008763:panose; D013395:Sucrose; D008320:Maltose; D016695:Glycosyltransferases