title: Measurement of estrogenic activity in sediments from Haihe and Dagu River, China.
abstract: Sediments from two rivers in China, the Haihe and Dagu Rivers, were examined for estrogenic activity using an estrogen receptor (ER)-mediated in vitro bioassay system. ER-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a florisil column into three fractions. The estrogenic activity of each extract was detected by measuring luciferase activity in the human breast cancer cell line MCF-7 transfected with a luciferase receptor gene. Significant estrogenic activity was observed in each total extract. The 17beta-estradiol equivalents (E2-EQs) ranged from 8.24 to 95.28 ng E2 g(-1) dw. As a result, the relative estrogenic potencies of three fractions in this study descended in the order of Fraction 3>Fraction 2>Fraction 1. The results of the bioassay analysis indicated the heavy pollution status of these sites with estrogenic contaminants. In this study, five selected chemicals, the natural estrogens 17beta-estradiol (E2) and estrone (E1), and the xeno-estrogens 4-octylphenol (OP), 4-nonylphenol (NP), and Bisphenol A (BPA) were also analyzed using the in vitro bioassay. The estrogenic activity of these chemicals were E2>E1>NP>OP>BPA.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085, China.
authors.forename: Maoyong
authors.initials: M
authors.lastname: Song
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Qinting
authors.initials: Q
authors.lastname: Jiang
authors.forename: Paul K S
authors.initials: PK
authors.lastname: Lam
authors.forename: Desmond K
authors.initials: DK
authors.lastname: O'Toole
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.forename: Guibin
authors.initials: G
authors.lastname: Jiang
mesh_terms: D001681:Biological Assay / Q000379:methods; D045744:Cell Line, Tumor; D002681:China; D004784:Environmental Monitoring / Q000379:methods; D004967:Estrogens / Q000032:analysis* / Q000737:chemistry; D005260:Female; D019015:Geologic Sediments / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D045483:Rivers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D004967:Estrogens; D014874:Water Pollutants, Chemical