Proteomic analysis of protein profiles during early development of the zebrafish, Danio rerio.
In the present study, profiles of protein expression were examined during early development of zebrafish, an increasingly popular experimental model in vertebrate development and human diseases. By 2-DE, an initial increase in protein spots from 6 h post-fertilization (hpf) to 8-10 hpf was observed. There was no dramatic change in protein profiles up to 18 hpf, but significant changes occurred in subsequent stages. Interestingly, 49% of the proteins detected at 6 hpf remained detectable by 1 week of age. To map the protein expression patterns in 2-D gels, MALDI-TOF/TOF MS was employed to identify selected protein spots from early embryos. 108 protein spots were found to match known proteins and they were derived from 55 distinct genes. Interestingly, 11 (20%) of them produced multiple protein isoforms or distinct cleavage products. Although deyolked embryos were used in the analysis, a large number of vitellogenin derivatives remained prominently present in the embryos. Other than these, most of the identified proteins are cytosolic, cytoskeletal and nuclear proteins, which are involved in diversified functions such as metabolism, cytoskeleton, translation, protein degradation, etc. Some of the proteins with interesting temporal expression profiles during development are further discussed.
Tay
Tuan Leng
TL
Department of Biological Sciences, National University of Singapore, Singapore 119260.
Lin
Qingsong
Q
Seow
Teck Keong
TK
Tan
Keng Hwa
KH
Hew
Choy Leong
CL
Gong
Zhiyuan
Z
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005775:Gastrula / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D007633:Keratins / Q000096:biosynthesis; D007814:Larva; D020033:Protein Isoforms / Q000096:biosynthesis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014819:Vitellogenins / Q000096:biosynthesis; D015027:Zebrafish / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000096:biosynthesis*
D006360:Heat-Shock Proteins; D020033:Protein Isoforms; D014819:Vitellogenins; D029961:Zebrafish Proteins; D007633:Keratins