Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216.
We isolated two lipase genes LIPY7, LIPY8 from Yarrowia lipolytica CGMCC (China general microbiological culture collection center) AS 2.1216. The LIPY7 and LIPY8 genes encode a 366 and a 371-amino acid protein, respectively. The lipase genes with 6 x His tag sequence were cloned into expression vector pPIC9K and successfully integrated into a heterologous fungal host Pichia pastoris KM71, respectively. The recombinants were induced by methanol to secrete active lipases into cultural medium. The recombinant lipases were also purified and characterized.
Song
Hui-Ting
HT
College of Chemistry and Material Science, Hubei University, Wuhan, PR China.
Jiang
Zheng-Bing
ZB
Ma
Li-Xin
LX
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D003001:Cloning, Molecular; D015870:Gene Expression; D008049:Lipase / Q000096:biosynthesis / Q000302:isolation & purification*; D010843:Pichia; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D025062:Yarrowia / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D008049:Lipase