title: Mannose hyperbranched dendritic polymers interact with clustered organization of DC-SIGN and inhibit gp120 binding.
abstract: DC-SIGN (dendritic cell-specific ICAM-3 grabbing non-integrin) is a C-type lectin receptor of dendritic cells and is involved in the initial steps of numerous infectious diseases. Surface plasmon resonance has been used to study the affinity of a glycodendritic polymer with 32 mannoses, to DC-SIGN. This glycodendrimer binds to DC-SIGN surfaces in the submicromolar range. This binding depends on a clustered organization of DC-SIGN mimicking its natural organization as microdomain in the dendritic cells plasma membrane. Moreover, this compound inhibits DC-SIGN binding to the HIV glycoprotein gp120 with an IC50 in the micromolar range and therefore can be considered as a potential antiviral drug.
authors.affiliation: Institut de Biologie Structurale, UMR 5075 CEA/CNRS/Université Joseph Fourier, 41, rue Jules Horowitz, 38027 Grenoble Cedex 1, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Tabarani
authors.forename: José J
authors.initials: JJ
authors.lastname: Reina
authors.forename: Christine
authors.initials: C
authors.lastname: Ebel
authors.forename: Corinne
authors.initials: C
authors.lastname: Vivès
authors.forename: Hugues
authors.initials: H
authors.lastname: Lortat-Jacob
authors.forename: Javier
authors.initials: J
authors.lastname: Rojo
authors.forename: Franck
authors.initials: F
authors.lastname: Fieschi
mesh_terms: D001704:Biopolymers / Q000737:chemistry* / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000187:drug effects* / Q000378:metabolism; D037181:Lectins, C-Type / Q000737:chemistry* / Q000378:metabolism; D008358:Mannose / Q000737:chemistry*; D015394:Molecular Structure; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D001704:Biopolymers; D015815:Cell Adhesion Molecules; D015699:HIV Envelope Protein gp120; D037181:Lectins, C-Type; D008358:Mannose; D011956:Receptors, Cell Surface; D012996:Solutions; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin