title: Mutation of p53 in skin papilloma and tubular breast adenoma of albino mice.
abstract: OBJECTIVE
  To determine the mutation of p53 in chemically induced carcinogenesis on albino mice in skin papilloma and tubular adenoma breast by immunohistochemistry.
  
  
  METHODS
  An experimental study.
  
  
  METHODS
  The animal house of Postgraduate Medical Institute and Pathology Department of King Edward Medical College University, Lahore, for the duration of 20 weeks, from 15 February, 2004 to 15 July, 2004.
  
  
  METHODS
  Twenty five albino mice (male and female) were selected for a study on chemical carcinogenesis. These animals were divided into five groups (A-E), five animals in each. DMBA (Dimethylebenz[a] Anthracene) and TPA (Tetradecanoyl-phorbal-13-Acetic Acid) [chemical carcinogens] were given to produce the tumors and mutation of p53 expression was evaluated on the tumors appearing during this period of carcinogenesis. Squamous cell papillomas and tubular adenoma breast were selected for this study.
  
  
  RESULTS
  All the papillomas showed faint reactivity for immunomarker p53, while tubular adenomas were nonreactive.
  
  
  CONCLUSIONS
  The results of this study show that p-53 is a marker for premalignant lesions and helps in selecting patients for constant monitoring, upon the clinical verification of these results.
authors.affiliation: Department of Pathology, KEMC University, Lahore. drmhbukhari@yahoo.com
authors.forename: Mulazim Hussain
authors.initials: MH
authors.lastname: Bukhari
authors.forename: Saroosh Majid
authors.initials: SM
authors.lastname: Salaria
authors.forename: Zafar
authors.initials: Z
authors.lastname: Niaz
authors.forename: Allyia
authors.initials: A
authors.lastname: Muzzafar
authors.forename: Zahra
authors.initials: Z
authors.lastname: Batool
authors.forename: G R
authors.initials: GR
authors.lastname: Qureshi
authors.forename: I A
authors.initials: IA
authors.lastname: Naveed
authors.forename: N A
authors.initials: NA
authors.lastname: Chaudhry
mesh_terms: D000236:Adenoma / Q000235:genetics* / Q000473:pathology; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D009374:Neoplasms, Experimental; D010212:Papilloma / Q000235:genetics* / Q000473:pathology; D011897:Random Allocation; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor