Characterization of human D-amino acid oxidase.
D-Amino acid oxidase (DAAO) has been proposed to be involved in the oxidation of D-serine, an allosteric activator of the NMDA-type glutamate receptor in the brain, and to be associated with the onset of schizophrenia. The recombinant human DAAO was expressed in Escherichia coli and was isolated as an active homodimeric flavoenzyme. It shows the properties of the dehydrogenase-oxidase class of flavoproteins, possesses a low kinetic efficiency, and follows a ternary complex (sequential) kinetic mechanism. In contrast to the other known DAAOs, the human enzyme is a stable homodimer even in the apoprotein form and weakly binds the cofactor in the free form.
Molla
Gianluca
G
Department of Biotechnology and Molecular Sciences, University of Insubria, Varese, Italy.
Sacchi
Silvia
S
Bernasconi
Mariagrazia
M
Pilone
Mirella S
MS
Fukui
Kiyoshi
K
Polegioni
Loredano
L
D001921:Brain / Q000201:enzymology; D003067:Coenzymes / Q000737:chemistry / Q000378:metabolism; D003605:D-Amino-Acid Oxidase / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D020536:Enzyme Activators / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D012559:Schizophrenia / Q000201:enzymology; D012694:Serine / Q000737:chemistry / Q000378:metabolism
D003067:Coenzymes; D020536:Enzyme Activators; D016194:Receptors, N-Methyl-D-Aspartate; D012694:Serine; C417983:DAO protein, human; D003605:D-Amino-Acid Oxidase