Murine strain differences in 8-hydroxy-deoxyguanosine formation in hepatic DNA induced by oxidized lard and dietary oils.
Difference of 8-hydroxy-deoxyguanosine (8-OH-dG) formation in liver DNA in C3H/HeN and in C57BL/6 mice--fed oxidized lard and dietary oils (soybean and sardine)--was investigated. The blank levels of 8-OH-dG were higher in C3H/HeN mice (highly sensitive to liver tumorigenesis) than in C57BL/6 mice (resistant strain). The level of 8-OH-dG increased much more in C3H/HeN mice than in the C57BL/6 mice fed by oxidized lard and dietary oil treatment. Feeding oxidized lard and dietary oils increased 8-oxo-guanine DNA glycosylase I (OGG1) and mRNA 8-oxo-dGTPase in C57BL/6 mice. On the other hand, no appreciable change of mRNA in the C3H/HeN mice was observed. The formation differences of 8-OH-dG from the two murine strains fed with oxidized lard and dietary oils may be associated with the different mRNA levels in the DNA repair enzymes because the mRNA levels in the DNA repair enzymes were much lower in C3H/HeN mice than in C57BL/6 mice.
Sadakane
K
K
Department of Health Sciences, Oita University of Nursing and Health Sciences, 2944-9, Notsuharu, Oita 870-1201, Japan.
Ichinose
T
T
Takano
H
H
Abe
M
M
Sera
N
N
Yanagisawa
R
R
Ochi
H
H
Fujioka
K
K
Lee
K-G
KG
Shibamoto
T
T
D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D045647:DNA Glycosylases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D045643:DNA Repair Enzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage / Q000633:toxicity*; D005395:Fish Oils / Q000008:administration & dosage / Q000633:toxicity; D006147:Guanine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008113:Liver Neoplasms / Q000139:chemically induced / Q000209:etiology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013024:Soybean Oil / Q000008:administration & dosage / Q000633:toxicity
D004041:Dietary Fats; D005395:Fish Oils; D012333:RNA, Messenger; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D013024:Soybean Oil; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D010744:Phosphoric Monoester Hydrolases; D045647:DNA Glycosylases; C485990:Ogg1 protein, mouse; C078207:8-oxodGTPase; D045643:DNA Repair Enzymes; C029310:lard