[High values of soluble cytokeratin 18 in breast carcinomas may have different meanings].
We have investigated the cellular and serum CK18 in 26 non-treated primary ductal invasive breast carcinomas. The soluble CK18 (TPS) was detected by chemiluminescent assay, and the cellular CK18 and PCNA expression by immunocytochemistry. Flow-cytometry was used to estimate the amount of DNA in malignant cells. There was a significant correlation between soluble CK18 and the pre-menopausal status (p < 0.05), characterized in our group by a PCNA estimated low proliferation index. We have also found a significant correlation between soluble CK18 and the DNA index (p < 0.01). The intracellular CK18 has correlated with the PCNA expression (p < 0.05), while no correlation could be found between cellular and serum CK18. The values of soluble CK18 may offer information about the treatment-induced cell death, if monitored, while isolated measurements should be interpreted cautiously. Elevated levels of serum CK18 in non-treated carcinomas may rather reflect a high tumor turn-over or perhaps a more intensive tumor cell killing.
Cianga
P
P
Disciplina Imunologie, Facultatea de Medicină, Universitatea de Medicină si Farmacie "Gr. T. Popa", Iaşi.
Cianga
Corina
C
Anisie
Ecaterina
E
Zlei
Mihaela
M
Ungureanu
Didona
D
Munteanu
Dorina
D
Chifu
Camelia
C
Diaconu
C
C
Carasevici
E
E
D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000097:blood* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000097:blood*; D008163:Luminescent Measurements; D010455:Peptides / Q000097:blood; D018809:Proliferating Cell Nuclear Antigen / Q000097:blood
D014408:Biomarkers, Tumor; D010455:Peptides; D018809:Proliferating Cell Nuclear Antigen; C089984:tissue polypeptide specific antigen; D007633:Keratins