title: Aminoacyl RNA domain of turnip yellow mosaic virus Val-RNA interacting with elongation factor Tu.
abstract: Turnip yellow mosaic virus (TYMV) Val-RNA forms a complex with the peptide elongation factor Tu (EF-Tu) in the presence of GTP: the Val-RNA is protected by EF-Tu.GTP from non-enzymatic deacylation and nuclease digestion. The determination of the length of the shortest TYMV Val-RNA fragment that binds EF-Tu.GTP leads us to conclude that the valylated aminoacyl RNA domain equivalent in tRNAs to the continuous helix formed by the acceptor stem and the T arm is sufficient for complex formation. Since the aminoacyl RNA domain is also sufficient for adenylation by the ATP(CTP):tRNA nucleotidyltransferase, an analogy can be drawn between these two tRNA-specific proteins.
authors.affiliation: Institut Jacques Monod, CNRS and Université Paris VII, 2 Place Jussieu, 75251 Paris Cedex 05, France.
authors.forename: R L
authors.initials: RL
authors.lastname: Joshi
authors.forename: H
authors.initials: H
authors.lastname: Faulhammer
authors.forename: F
authors.initials: F
authors.lastname: Chapeville
authors.forename: M
authors.initials: M
authors.lastname: Sprinzl
authors.forename: A L
authors.initials: AL
authors.lastname: Haenni