Effect of partially hydrolyzed soluble glucan produced by glucosyltrasferases of Streptococcus mutans on stimulating human T cell.
Soluble glucan, which was obtained from action of glucosyltransferases (GTFs) of Streptococcus mutans on sucrose, was partially hydrolyzed by acetic acid and examined for human T lymphoblast (MOLT-4) stimulating activity. Addition of the partially hydrolyzed glucan (15-60 microg/ml) stimulated human T cell (39-65%) in a dose dependant manner according to MTT assay. Production of interleukine-2 (IL-2) and interleukine-2 receptor (IL-2R) from T cell was increased by 44.5 and 25%, respectively, by addition of partially hydrolyzed glucan (15 microg/ml). These results indicate that stimulation of human T cells by hydrolyzed glucan is probably caused by its effects on stimulating gene expression of IL-2 and IL-2R of human T cell.
Choi
Inwook
I
Korea Food Research Institute, San 46-1, Bundang-gu, Sungnam-si, Kyunggi-do, 463-746, Korea. choiw@kfri.re.kr
Jung
Changhwa
C
Han
Yeook
Y
Lee
Eunjoo H
EH
D019342:Acetic Acid / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D005936:Glucans / Q000096:biosynthesis* / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation / Q000187:drug effects; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012995:Solubility; D013295:Streptococcus mutans / Q000201:enzymology*; D013395:Sucrose / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
D005936:Glucans; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D013395:Sucrose; D005964:Glucosyltransferases; D019342:Acetic Acid