[Effect of heat and BPDE on the expression of Hsp27 and Hsp70 in A549 cell line].
OBJECTIVE
To investigate the expression of the Hsp27 and Hsp70 in A549 cells treated with heat and BPDE.


METHODS
A549 cells cultured in vitro were divided into heat stress group and BPDE group. Cells cultured at 37 degrees C used as control. The heat stress group was exposed to heat at different temperature (39 degrees C, 42 degrees C and 43 degrees C) for 2h. The BPDE group was treated with different concentrations of BPDE (0, 2, 4 and 8 micromol/L). Western-blot was used for the Hsp27 and Hsp70 expression analysis.


RESULTS
In heat stress group, Hsp27 expression levels significantly increased compared to the control (P < 0.05) and reached its peak at 39 degrees C. Hsp70 expression level also increased after heat stress. It reached the peak at 42 degrees C and was higher than that of the control with significance (P < 0.05). In BPDE group, Hsp27 and Hsp70 expression levels were higher than those of the control. Hsp27 reached a maximum at 8 micromol/L, the highest concentration of BPDE in this experiment, and was significantly higher than that of the control (P < 0.05). But it was still on its elevatory phase. Hsp70 reached its peak at 4 micromol/L and was significantly higher than that of the control at 4 micromol/L and 8 micromol/L.


CONCLUSIONS
Both heat and BPDE can induce the expressions of Hsp27 and Hsp70. In heat stress group, the Hsp27 and Hsp70 levels reached their peaks at 39 degrees C and 42 degrees C. In BPDE group, the expressional levels of Hsp27 and Hsp70 reached their peaks at 8 micromol/L and 4 micromol/L.
Duan
Yan-Ying
YY
School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Long
Zong-Yan
ZY
Niu
Pi-Ye
PY
Wu
Tang-Chu
TC
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000633:toxicity*; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D002273:Carcinogens / Q000633:toxicity; D045744:Cell Line, Tumor; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006358:Hot Temperature; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D018832:Molecular Chaperones
D002273:Carcinogens; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide