Development of an HPLC method for the determination of nifedipine in human plasma by solid-phase extraction.
Nifedipine, a dihydropyridine calcium channel antagonist, is widely used in the treatment of hypertension and other cardiovascular disorders. A selective, sensitive and accurate high-performance liquid chromatographic method has been developed, validated and applied for determination of nifedipine in human plasma samples. A series of studies were conducted in order to investigate the effects of mobile phase composition, buffer concentration, mobile phase pH and concentration of organic modifiers, and to develop a convenient and easy-to-use method for quantitative analysis of nifedipine. The method involves solid-phase extraction on C18 cartridges. The chromatographic separation was accomplished on a Lichrocart Lichrospher 60 RP selectB column with a mobile phase composed of 0.020 mol/L KH2PO4 (pH 4.8) and acetonitrile (42:58, v/v). UV detection was set at 240 nm. The calibration curve was linear in the concentration range of 5.0-200.0 ng/mL for nifedipine in plasma and the limit of quantification was 5.0 ng/mL.
Zendelovska
Dragica
D
Institute of Preclinical and Clinical Pharmacology and Toxicology, St. Cyril and Methodius University, Medical Faculty, 50 Divizija bb, 1000 Skopje, Macedonia. pharma@on.net.mk
Simeska
Suzana
S
Sibinovska
Olgica
O
Kostova
Elena
E
Milosevska
Kalina
K
Jakovski
Krume
K
Jovanovska
Emilija
E
Kikerkov
Igor
I
Trojacanec
Jasmina
J
Zafirov
Dimce
D
D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009543:Nifedipine / Q000097:blood* / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results
D009543:Nifedipine