Determination of the 5'- and 3'-terminal sequences completes the sequences of the two double-stranded RNAs of Penicillium stoloniferum virus S.
The two genomic segments of Penicillium Stoloniferum virus S (PsV-S), a member of the Partitiviridae, were recently sequenced and published. We independantly sequenced PsV-S and showed that the original sequence was missing nucleotides at both the 5' and 3' termini of both segments. We determined the correct sequence in three independent experiments and found the segments to be 1753 bp (encoding the RNA-dependant RNA polymerase) and 1581 bp (encoding the Capsid Protein). Homology was shown between the 5' and 3' ends of PsV-S and other members of the Partitiviridae.
Hacker
Caroline V
CV
Faculty of Life Sciences, Division of Biology, Imperial College, London, UK. c.hacker@oxfordbiomedica.co.uk
Brasier
Clive M
CM
Buck
Kenneth W
KW
D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010407:Penicillium / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid
D036022:Capsid Proteins; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase