Carbohydrate recognition by Clostridium difficile toxin A.
Clostridium difficile TcdA is a large toxin that binds carbohydrates on intestinal epithelial cells. A 2-A resolution cocrystal structure reveals two molecules of alpha-Gal-(1,3)-beta-Gal-(1,4)-beta-GlcNAcO(CH(2))(8)CO(2)CH(3) binding in an extended conformation to TcdA. Residues forming key contacts with the trisaccharides are conserved in all seven putative binding sites in TcdA, suggesting a mode of multivalent binding that may be exploited for the rational design of novel therapeutics.
Greco
Antonio
A
Alberta Ingenuity Centre for Carbohydrate Science, University of Calgary, Calgary, Canada T2N 1N4.
Ho
Jason G S
JG
Lin
Shuang-Jun
SJ
Palcic
Monica M
MM
Rupnik
Maja
M
Ng
Kenneth K-S
KK
D001427:Bacterial Toxins / Q000737:chemistry* / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D016360:Clostridioides difficile / Q000737:chemistry* / Q000378:metabolism*; D004768:Enterotoxins / Q000737:chemistry* / Q000378:metabolism*; D006860:Hydrogen Bonding; D008958:Models, Molecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary
D001427:Bacterial Toxins; D002241:Carbohydrates; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile