High-throughput RNA interference in functional genomics.
RNA interference (RNAi) refers to post-transcriptional silencing of gene expression as a result of the introduction of double-stranded RNA into cells. The application of RNAi in experimental systems has significantly accelerated elucidation of gene functions. In order to facilitate large-scale functional genomics studies using RNAi, several high-throughput approaches have been developed based on microarray or microwell assays. The recent establishment of large libraries of RNAi reagents combined with a variety of detection assays has further improved the performance of functional genome-wide screens in mammalian cells.
Janitz
M
M
Department Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Fabeckstr. 60-62, 14195 Berlin, Germany. janitz@molgen.mpg.de
Vanhecke
D
D
Lehrach
H
H
D000818:Animals; D020868:Gene Silencing; D023281:Genomics; D006801:Humans; D034622:RNA Interference / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D034743:RNA-Induced Silencing Complex / Q000235:genetics / Q000502:physiology
D034741:RNA, Small Interfering; D034743:RNA-Induced Silencing Complex