title: [Expression of tissue factor induced by IL-6 in HUVEC].
abstract: OBJECTIVE
  To investigate the effects of IL-6 at the expression of tissue factor (TF) in human vein endothelial cells(HUVECs).
  
  
  METHODS
  HUVECs were incubated with IL-6 at the concentration of 0.5 ng/mL. Cell viability was measured by CCK-8 assay. The TF mRNA was detected by reverse transcript-polymerase chain reaction(RT-PCR) method.
  
  
  RESULTS
  When HUVECs were exposed to IL-6 (0.5 ng/mL) within a period of 72 h, their viability did not decrease in comparison with the control; there was no statistical difference between the two groups. After the HUVECs were exposed to IL-6 (0.5 ng/mL) for 6 h, the TF mRNA level increased, and it reached the peak at 12 h; then it began to decline. The expression of TF mRNA induced by IL-6 was evidently detected from 6 h to 48 h. After the HUVECs were treated by IL-6 over 72 h, the expression of TF mRNA was no longer detected in HUVECs.
  
  
  CONCLUSIONS
  IL-6 at the concentration of 0.5 ng/mL did not exert direct effect on cell viability. The increase of TF mRNA expression in HUVECs induced by IL-6 could play an important role in the modulation of blood coagulation disorder and in the mechanism related to coagulation system changes during
authors.affiliation: Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Tang
authors.forename: Zhen-You
authors.initials: ZY
authors.lastname: Jiang
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
authors.forename: Xiao-Cheng
authors.initials: XC
authors.lastname: Liu
authors.forename: Shu-Yu
authors.initials: SY
authors.lastname: Cai
authors.forename: Rui
authors.initials: R
authors.lastname: Xiao
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Lu
mesh_terms: D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013925:Thromboplastin / Q000096:biosynthesis* / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D012333:RNA, Messenger; D013925:Thromboplastin