title: Visualization of molecular interactions by fluorescence complementation.
abstract: The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides.
authors.affiliation: Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0650, USA. kerppola@umich.edu
authors.forename: Tom K
authors.initials: TK
authors.lastname: Kerppola
mesh_terms: D008856:Microscopy, Fluorescence; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D011506:Proteins; D025801:Ubiquitin