Identification of CD36 as a new surface marker of marginal zone B cells by transcriptomic analysis.
Follicular (FO) B cells and marginal zone (MZ) B cells belong to the mature B cell population in spleen of mice. To identify new surface markers of these mature B cell subsets, we compared gene expression profiles of FO and MZ B cells by DNA microarray using FACS-sorted mouse FO and MZ B cells. From 14,000 mouse genes, 27 membrane proteins were expressed mainly in MZ B cells while another 22 membrane proteins expressed largely in FO B cells. Using FACS analysis, we identified that CD36, CD68, and CD49e were expressed on MZ B cells but not on FO B cells. In addition, using semi-quantitative PCR, we found that the mRNA of CD131 were much more abundant in MZ B cells. These results revealed new phenotypic properties of MZ and FO B cells, and would facilitate further studies in the differentiation and functions of these mature B cells.
Zhang
Ping
P
State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, and Department of Dermatology, Xijng Hospital, Xi'an 710032, China.
Li
Wei
W
Wang
Yaochun
Y
Hou
Lihong
L
Xing
Ying
Y
Qin
Hongyan
H
Wang
Jishu
J
Liang
Yingmin
Y
Han
Hua
H
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D015415:Biomarkers; D018955:CD36 Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014158:Transcription, Genetic
D000954:Antigens, Surface; D015415:Biomarkers; D018955:CD36 Antigens