Real-time in vivo blood-flow imaging by moving-scatterer-sensitive spectral-domain optical Doppler tomography.
We present a moving-scatterer-sensitive optical Doppler tomography (MSS-ODT) technique for in vivo blood flow imaging in real time by using a spectral-domain optical coherence tomography system. In MSS-ODT the influence of stationary scatterers is suppressed by subtracting adjacent complex axial scans before calculating the Doppler frequency shift. We demonstrate that MSS-ODT is a useful technique for accurate determination of blood vessel size by imaging flow in a small capillary tube with a 75 microm inner diameter. The flow profile obtained with MSS-ODT yields a substantially more accurate tube diameter than that obtained with the conventional phase-resolved method, which underestimates the diameter by about 23%. We also demonstrate that MSS-ODT provides improved sensitivity over the conventional phase-resolved method for imaging in vivo blood flow in small vessels in a mouse ear.
Ren
Hongwu
H
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA. hwren@u.washington.edu
Sun
Tao
T
MacDonald
Daniel J
DJ
Cobb
Michael J
MJ
Li
Xingde
X
D000465:Algorithms; D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D003199:Computer Systems; D004423:Ear / Q000098:blood supply* / Q000502:physiology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008027:Light; D051379:Mice; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000379:methods*