[Cathepsin B expression and malignances and angiogenesis in gliomas].
OBJECTIVE
To test the expression of Cathepsin B (CB) protease in gliomas and to investigate its correlation with the malignance and angiogenesis of gliomas.


METHODS
Immunochemistry technique of strept actividin-biotin complex (SABC) was used to identify the CB expression and to measure the microvessel density (MVD) in 47 gliomas samples and 5 normal human brain tissues from patients with spontaneous cerebral hemorrhage.


RESULTS
No CB expression was detected in the normal brain tissues. The expression levels of CB in astrocytoma, anaplastoma, and glioblastoma were 30% (6/20 weak positive), 86.7% (3/15 weak and, 10/15 moderate positive), and 100.0% (2/12 moderate and 10/12 strong positive), respectively. The malignances of gliomas increased with CB expression (r=0. 77, P<0.05). The MVD also increased with CB expression (r= 0.56, P<0. 05), with an average of 15.57 +/- 8.52, 22.50 +/- 14.42, 39.85 +/- 8.75 and 49.90 +/- 11.30 for the negative, weak, moderate, and strong CB expression gliomas respectively.


CONCLUSIONS
CB expression is positively correlated with the malignances and angiogenesis of gliomas. CB expression could be one of the indicators for the degree of malignances and invasion of gliomas.
Tang
Jian-Jian
JJ
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, China.
Jiang
Shu
S
Mao
Bo-Yong
BY
D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology*; D002401:Cathepsin B / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005909:Glioblastoma / Q000378:metabolism; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D002401:Cathepsin B