title: Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil).
abstract: In order to evaluate the taeniosis-cysticercosis complex in a population of a peasants' settlement, located at Teodoro Sampaio, state of São Paulo, Brazil (longitude 52 degrees 36'12 ", latitude 22 degrees 17'12 ") a series of laboratory markers were determined. After signing an informed consent, participants answered a standardized questionnaire. To determine anti-Taenia solium cysticercus antibodies, the samples were tested by enzyme linked immunoabsorbent assay using 18-and 14-kDa antigen proteins from vesicular fluid of Taenia crassiceps (VF-Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Total IgE levels were determined by chemmiluminescence's assay and hemogram by flow cytometer flux counter. A total of 84 individuals, 5.9% presented anti-T. solium cysticercus antibodies in ELISA and 3.6% were strongly reactive in the 18/14 kDa immunoblotting confirmatory test. All of the individuals with positive antibodies showed elevated Total IgE levels. We conclude that the frequency of anti-T. solium cysticercus antibodies in this population is higher than other regions considered endemic in São Paulo. Thus, it is important to carry out surveys in Peasants' settlement areas with the objective of establishing public health measures for prevention and control of infectious diseases such as taeniosis-cysticercosis.
authors.affiliation: Departamento de Imunologia, Universidade do Oeste Paulista, 19050-900 Presidente Prudente, SP, Brazil. luiz@unoeste.br
authors.forename: Luiz Euribel
authors.initials: LE
authors.lastname: Prestes-Carneiro
authors.forename: Selma de Bastos Zambeli de
authors.initials: Sde B
authors.lastname: Freitas
authors.forename: Sueli Cristina Schadeck
authors.initials: SC
authors.lastname: Zago
authors.forename: Nadia Araújo
authors.initials: NA
authors.lastname: Miguel
authors.forename: Oswaldo Bill
authors.initials: OB
authors.lastname: Primo
authors.forename: Alberto Hiroshi
authors.initials: AH
authors.lastname: Iha
authors.forename: Noeli Maria
authors.initials: NM
authors.lastname: Espíndola
authors.forename: Adelaide José
authors.initials: AJ
authors.lastname: Vaz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001938:Brazil / Q000453:epidemiology; D002648:Child; D003551:Cysticercosis / Q000175:diagnosis* / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood*; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D013622:Taeniasis / Q000175:diagnosis* / Q000453:epidemiology
chemical_list: D000909:Antibodies, Helminth; D015415:Biomarkers; D007073:Immunoglobulin E