title: Crystals of sarcoplasmic reticulum Ca(2+)-ATPase.
abstract: High-resolution structures of the Ca(2+)-ATPase have over the last 5 years added a structural dimension to our understanding of the function of this integral membrane protein. The Ca(2+)-ATPase is now by far the membrane protein where the most functionally different conformations have been described in precise structural detail. Here, we review our experience from solving Ca(2+)-ATPase structures: a purification scheme involving minimum handling of the protein to preserve natural and essential lipids, a rational approach to screening for crystals based on a limited number of polyethyleneglycols and many different salts, improving crystal quality using additives, collecting the data and finally solving the structures. We argue that certain of the lessons learned in the present study are very likely to be useful for crystallisation of eukaryotic membrane proteins in general.
authors.affiliation: Department of Molecular Biology, Aarhus University, Denmark.
authors.forename: Thomas Lykke-Møller
authors.initials: TL
authors.lastname: Sørensen
authors.forename: Claus
authors.initials: C
authors.lastname: Olesen
authors.forename: Anne-Marie Lund
authors.initials: AM
authors.lastname: Jensen
authors.forename: Jesper Vuust
authors.initials: JV
authors.lastname: Møller
authors.forename: Poul
authors.initials: P
authors.lastname: Nissen
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000737:chemistry* / Q000302:isolation & purification; D018360:Crystallography, X-Ray / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*
chemical_list: D000252:Calcium-Transporting ATPases