title: Molecular response of leukemia HL-60 cells to genistein treatment, a proteomics study.
abstract: Genistein (GEN) is a natural protein tyrosine kinase inhibitor. We analyzed the molecular response of HL-60 cells to GEN treatment by gel-based proteomics approach. Fourteen differentially expressed proteins which are functionally involved in metabolism, cell signaling, RNA processing, cell proliferation and motility, and chaperones were identified. Both the dose- and time-dependent up-regulation of Hsp70 protein 8 and heterogeneous nuclear ribonucleoprotein (hnRNP) H1, and the down-regulation of Rab14, hnRNP C and stathmin-1 by GEN were verified by immunoblot analysis. Our novel findings provide insightful clues to the potential therapeutic targets for leukemia treatment in diverse tyrosine kinase-dependent cellular pathways.
authors.affiliation: Department of Laboratory Medicine, National University Hospital, 5 Lower Kent Ridge, Singapore 119074, Singapore.
authors.forename: Daohai
authors.initials: D
authors.lastname: Zhang
authors.forename: Yan-Chin
authors.initials: YC
authors.lastname: Tai
authors.forename: Ching-Ho Stephen
authors.initials: CH
authors.lastname: Wong
authors.forename: Lee-Kian
authors.initials: LK
authors.lastname: Tai
authors.forename: Evelyn Siew-Chuan
authors.initials: ES
authors.lastname: Koay
authors.forename: Chien-Shing
authors.initials: CS
authors.lastname: Chen
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D040901:Proteomics; D015203:Reproducibility of Results; D012261:Ribonucleoproteins / Q000235:genetics / Q000302:isolation & purification; D014357:Trypsin
chemical_list: D016588:Anticarcinogenic Agents; D004791:Enzyme Inhibitors; D009363:Neoplasm Proteins; D012261:Ribonucleoproteins; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D014357:Trypsin