title: [Application of micronucleus test of buccal mucosal cells in assessing the genetic damage of workers exposed to acrylonitrile].
abstract: OBJECTIVE
  To investigate the application of micronucleus test of buccal mucosal cells in monitoring the genetic effect of acrylonitrile in the population exposed to the acrylonitrile.
  
  
  METHODS
  Forty-one healthy male workers in a chemical factory in Shanghai were selected as the low concentration acrylonitrile exposed group while forty-seven healthy male workers in an acrylonitrile factory in Shanghai were selected as the intermediate concentration acrylonitrile exposed group. At the same time, thirty-one male workers who had no toxicant exposure and lived in the same community were selected as the control group. The micronucleus test in buccal mucosal cells and lymphocytes were used respectively for assessing the genetic damage status of these men.
  
  
  RESULTS
  The rate of micronucleus in buccal mucosal cells in both acrylonitrile groups (the low concentration group: 3.68% +/- 2.72%; the intermediate concentration group: 4.00% +/- 2.38%) was significantly higher than that in the control group (2.03% +/- 2.20%) (P < 0.05). The rate of micronucleus in the intermediate concentration group (4.23% +/- 3.34%) was significantly higher than that in the control group (2.48% +/- 1.46%) (P < 0.05). There was the correlation between the micronucleus test of buccal mucosal cells and the micronucleus test of the lymphocytes in the peripheral blood in the acrylonitrile exposed population (r = 0.299-0.359, P < 0.05).
  
  
  CONCLUSIONS
  The micronucleus test of buccal mucosal cells replacing the micronucleus test of the lymphocytes in the peripheral blood can be used as one of the screening indexes in the surveillance of the genetic damage in the acrylonitrile exposed population.
authors.affiliation: Department of Occupational Health, Jin Shan Hospital, Fudan University, Shanghai 200540, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fan
authors.forename: Wei-lan
authors.initials: WL
authors.lastname: Wang
authors.forename: Sheng
authors.initials: S
authors.lastname: Ding
authors.forename: Yuan-ling
authors.initials: YL
authors.lastname: Zhou
authors.forename: Fu-sheng
authors.initials: FS
authors.lastname: Jin
mesh_terms: D000181:Acrylonitrile / Q000633:toxicity*; D000328:Adult; D002273:Carcinogens / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D009061:Mouth Mucosa / Q000166:cytology*; D016273:Occupational Exposure
chemical_list: D002273:Carcinogens; D000181:Acrylonitrile