title: Incorporation of a flared inlet capillary tube on a fourier transform ion cyclotron resonance mass spectrometer.
abstract: Flared inlet capillary tubes have been coupled with a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer to help the ion transmission from the atmospheric pressure to the first vacuum region. We investigated different types of atmospheric pressure ionization methods using flared inlet tubes. For most of the ionization methods, such as ESI and DESI, increased ion current transmitted from the atmospheric pressure ion source to the first stage vacuum system was observed with the use of our enhanced ion inlet designs. The corresponding ion intensity detected on a FT-ICR mass spectrometer was also observed to increase two- to fivefold using ESI or DESI with the flared tube inlet. Moreover, increased spray tip positional tolerance was observed with implementation of the flared inlet tube. We also include our preliminary results obtained by coupling AP-MALDI with flared inlet tube in this paper. For AP-MALDI, the measured ion current transferred through the flared inlet tube was about 2 to 3 times larger than the ion current through the control non-flared inlet tube.
authors.affiliation: Department of Chemistry, Washington State University, P.O. Box 644630, 99164-4630, Pullman, WA, USA.
authors.forename: Si
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Washington State University, P.O. Box 644630, 99164-4630, Pullman, WA, USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Washington State University, P.O. Box 644630, 99164-4630, Pullman, WA, USA.
authors.forename: Nathan K
authors.initials: NK
authors.lastname: Kaiser
authors.affiliation: Department of Chemistry, Washington State University, P.O. Box 644630, 99164-4630, Pullman, WA, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Bruce
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Prior
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Anderson
mesh_terms: D000809:Angiotensins / Q000032:analysis; D001274:Atmospheric Pressure; D017357:Cyclotrons / Q000295:instrumentation; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation* / Q000379:methods; D013373:Substance P / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000295:instrumentation* / Q000379:methods
chemical_list: D000809:Angiotensins; D013373:Substance P