title: Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry.
abstract: Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.
authors.affiliation: Lawrence Livermore National Laboratory, Livermore, California, USA.
authors.forename: Herbert J
authors.initials: HJ
authors.lastname: Tobias
authors.forename: Maurice E
authors.initials: ME
authors.lastname: Pitesky
authors.forename: David P
authors.initials: DP
authors.lastname: Fergenson
authors.forename: Paul T
authors.initials: PT
authors.lastname: Steele
authors.forename: Joanne
authors.initials: J
authors.lastname: Horn
authors.forename: Matthias
authors.initials: M
authors.lastname: Frank
authors.forename: Eric E
authors.initials: EE
authors.lastname: Gard
mesh_terms: D000336:Aerosols; D001407:Bacillus / Q000737:chemistry / Q000254:growth & development*; D013058:Mass Spectrometry / Q000379:methods*; D013171:Spores, Bacterial / Q000254:growth & development
chemical_list: D000336:Aerosols