[Effect of ginkgo biloba extract and dipyridamole on transcription and translation of inducible NO synthbase in rabbits after myocardial ischemia-reperfusion injury].
OBJECTIVE
To investigate the effects of Egb761, an extract of ginkgo biloba , and dipyridamole on inducible NO synthase (iNOS) in rabbits after myocardial ischemia-reperfusion injury.


METHODS
After being established into ischemia-reperfusion injury model, 35 rabbits were divided randomly into 5 groups: Group A (the sham group), Group B (the model group), Group C (treated with dipyridamole 0.8 mg/kg), Group D (treated with Egb761, 40 mg/kg), and Group E (treated with Egb761 40 mg/kg combined with dipyridamole 0.8 mg/kg), all the medications were administered by intravenous injection 30 min after reperfusion. After administration, myocardial iNOS mRNA expression was detected by RT-PCR and western blot.


RESULTS
Myocardial iNOS mRNA transcriptive expression in the 5 groups were A 0, B 157.11 +/- 17.73, C 202.6 +/- 21.84, D 356.13 +/- 24.18 and E 562.34 +/- 35.19 respectively, showing significant difference between the treated groups and group B (P <0.01). The translative expression of myocardial iNOS in the 5 groups were A 34.24 +/- 15.78, B 75.70 +/- 13.71, C 116.89 +/- 22.57, D 143.75 +/- 16.05 and E 195.09 +/- 22.25 respectively, showing significant difference between the treated groups and group B as well (P < 0.05, P < 0.01).


CONCLUSIONS
Both Egb761 and dipyridamole could increase myocardial iNOS expression in transcriptive and translative levels in rabbits after myocardial ischemia-reperfusion injury, and the combined treatment of them shows a more significant effect.
Song
Qing-Jiang
QJ
Department of Internal Medicine, Zibo Hospital of Traditional Chinese Medicine Affiliated to Shandong University of TCM, Shandong (255300).
Wang
Shao-Hua
SH
Yang
Jie
J
Sun
Jie
J
Yan
Quanjian
Q
Zhu
Miaozhang
M
Guo
Zhikun
Z
Chen
Zhiheng
Z
D000818:Animals; D004176:Dipyridamole / Q000627:therapeutic use*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D005260:Female; D020441:Ginkgo biloba; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics; D009206:Myocardium / Q000201:enzymology; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics*; D008517:Phytotherapy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits; D011897:Random Allocation; D014158:Transcription, Genetic
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D004176:Dipyridamole; D052247:Nitric Oxide Synthase Type II