title: Improved method for the determination of hydroxymethylfurfural in baby foods using liquid chromatography-mass spectrometry.
abstract: An improved analytical method for the rapid, reliable, and sensitive determination of hydroxymethylfurfural (HMF) in baby foods is described. It entailed aqueous extraction from food matrix with simultaneous clarification using Carrez I and II reagents, solid-phase extraction cleanup using Oasis HLB, and analysis by liquid chromatography-mass spectrometry. A narrow-bore column allowed fast chromatographic separation with good resolution of HMF and matrix coextractives. In positive atmospheric pressure chemical ionization conditions, precursor and compound-specific ions were sensitively detected in selected ion monitoring mode. Sample preparation with efficient cleanup followed by fast chromatographic analysis allowed the analysis to be completed in <20 min. Recovery ranged between 91.8 and 94.7% for spiking levels of 0.25, 1.0, and 5.0 mg/kg HMF in cereal-based baby foods. The method was shown to be successful when using liquid chromatography coupled to ultraviolet detection at 285 nm.
authors.affiliation: Department of Food Engineering, Hacettepe University, 06532 Beytepe, Ankara, Turkey. vgokmen@hacettepe.edu.tr
authors.forename: Vural
authors.initials: V
authors.lastname: Gökmen
authors.forename: Hamide Z
authors.initials: HZ
authors.lastname: Senyuva
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D007202:Indicators and Reagents; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde