Proteomic analysis of rat pheochromocytoma PC12 cells.
PC12 cell line is well documented and widely applied as many kinds of models in neurobiological and neurochemical studies. Yet a thorough proteomic analysis has not been performed so far. Here we report the construction of a large-scale 2-D protein database for PC12 cells. The proteins extracted from PC12 cells were separated by 2-DE and identified by MALDI-TOF/TOF MS. A total of 1080 protein spots, excised from three different 2-D gels, were identified with high confidence. These proteins represent 474 different gene products, mainly binding proteins and enzymes. Three hundred and seven identified protein spots were located in the low-molecular weight region below 20 kDa. This database today represents one of the largest 2-D databases for higher eukaryotic cell proteomes and for low-molecular weight proteins. In addition, fragment ion spectra obtained by TOF/TOF confirmed that calcylin in PC12 cells was N-acetylated. The database of PC12 proteome is expected to be a powerful tool for neuroscientists.
Yang
Wei
W
Department of Biochemistry and Molecular Biology, College of Life Sciences, National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, P. R. China.
Liu
Peng
P
Liu
Yashu
Y
Wang
Qingsong
Q
Tong
Yuanpeng
Y
Ji
Jianguo
J
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D016716:PC12 Cells; D010673:Pheochromocytoma; D020543:Proteome / Q000032:analysis*; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome