title: Interleukin-2 gene-encoded stromal cells inhibit the growth of metastatic cholangiocarcinomas.
abstract: OBJECTIVE
  To demonstrate bone marrow stromal cells (BMSCs) can be used as an attractive target for genetic modification in the treatment of malignant diseases.
  
  
  METHODS
  Using a hamster model of biliary cancer, we investigated the therapeutic effects of interleukin-2 (IL-2) gene-modified BMSCs. Syrian golden hamsters were injected via the femoral vein with 5 x 10(5) cells of the KIGB-5 biliary cancer cell line (n=20). One week later, the hamsters were injected intraperitoneally with BMSCs containing Ad/hIL-2 and Ad/ Delta E1, unmodified BMSCs, or RPMI only (control) and observed for 12 wk (n=5 /each group).
  
  
  RESULTS
  All hamsters treated with BMSCs containing Ad/hIL-2 survived with no evidence of the disease during this period. In contrast, hamsters in the other three groups showed disseminated metastases involving the lungs as early as 4 wk.
  
  
  CONCLUSIONS
  Ad/IL-2 therapy is effective in the treatment of biliary cancer.
authors.affiliation: Department of Gastroenterology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: Myung-Hwan
authors.initials: MH
authors.lastname: Kim
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Lee
authors.forename: Sung Koo
authors.initials: SK
authors.lastname: Lee
authors.forename: Seung-Gyu
authors.initials: SG
authors.lastname: Lee
authors.forename: Chul-Won
authors.initials: CW
authors.lastname: Suh
authors.forename: Gyung-Yub
authors.initials: GY
authors.lastname: Gong
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Young-Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms / Q000473:pathology / Q000628:therapy*; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000556:secondary / Q000628:therapy*; D006224:Cricetinae; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D008175:Lung Neoplasms / Q000556:secondary; D008647:Mesocricetus; D017154:Stromal Cells / Q000502:physiology*; D014161:Transduction, Genetic; D023041:Xenograft Model Antitumor Assays
chemical_list: D007376:Interleukin-2