Delivery of sphingosine 1-phosphate from poly(ethylene glycol) hydrogels.
While protein growth factors promote therapeutic angiogenesis, delivery of lipid factors such as sphingosine 1-phosphate (S1P) may provide better stabilization of newly formed vessels. We developed a biomaterial for the controlled delivery of S1P, a bioactive lipid released from activated platelets. Multiarm poly(ethylene glycol)-vinyl sulfone was cross-linked with albumin, a lipid-transporting protein, to form hydrogels. The rate of S1P release from the materials followed Fickian kinetics and was dependent upon the presence of lipid carriers in the release solution. Delivery of S1P from RGD-modified hydrogels increased the cell migration speed of endothelial cells growing on the materials. The materials also induced angiogenesis in the chorioallantoic membrane assay. Our data demonstrate that the storage and release of lipid factors provides a new route for the induction of angiogenesis by artificial materials.
Wacker
Bradley K
BK
Department of Biomedical Engineering, Washington University in St. Louis, One Brookings Drive, St. Louis, Missouri 63130, USA.
Scott
Evan A
EA
Kaneda
Megan M
MM
Alford
Shannon K
SK
Elbert
Donald L
DL
D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D005782:Gels / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D008246:Lysophospholipids / Q000737:chemistry*; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000737:chemistry; D013997:Time Factors
D005782:Gels; D020100:Hydrogels; D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; D011092:Polyethylene Glycols; D013110:Sphingosine