title: Electrofocusing of methanolic extracts for identification of individual flavonol biomolecules in Camellia species.
abstract: An effort has been made to isolate individual catechin compounds from green tea leaves in their pure form by electrophoresis. In the present study total polyphenol extraction was carried out initially and estimated through spectrophotometric and HPLC methods. Extracted polyphenol was separated on 0.7% agarose gel and visualized at 360 nm. Fragmented individual compounds were gel eluted with methanol and confirmed as (-)-epigallocatechin (EGC), (-)-epicatechin (EC), (-)-epicatechin gallate (ECG), and (-)-epigallocatechin gallate (EGCG) by HPLC. The method developed describes a suitable method for the isolation of valuable molecules in tea.
authors.affiliation: Division of Plant Physiology, UPASI Tea Research Institute, Nirar Dam BPO, Valparai, 642 127 TN, India. johnupasi@rediffmail.com
authors.forename: K M Mariya
authors.initials: KM
authors.lastname: John
authors.forename: Deepu
authors.initials: D
authors.lastname: Vijayan
authors.forename: P R
authors.initials: PR
authors.lastname: Rahul
authors.forename: S D
authors.initials: SD
authors.lastname: Joshi
authors.forename: R Raj
authors.initials: RR
authors.lastname: Kumar
authors.forename: A K A
authors.initials: AK
authors.lastname: Mandal
mesh_terms: D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D044948:Flavonols / Q000032:analysis*; D007525:Isoelectric Focusing; D000432:Methanol; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D013053:Spectrophotometry
chemical_list: D044948:Flavonols; D010936:Plant Extracts; D002392:Catechin; C062669:epicatechin gallate; C045651:epigallocatechin gallate; C057580:gallocatechol; D000432:Methanol