Detection of recurrent copy number loss at Yp11.2 involving TSPY gene cluster in prostate cancer using array-based comparative genomic hybridization.
Prostate cancer is the second leading cause of cancer deaths among American men. The loss of Y chromosome has been frequently observed in primary prostate cancer as well as other types of cancer. Earlier, we showed that introduction of the human Y chromosome suppresses the in vivo tumorigenicity of the prostate cancer cell line PC-3. To further characterize the Y chromosome, we have developed a high-density bacterial artificial chromosome (BAC) microarray containing 178 BAC clones from the human Y chromosome. BAC microarray was used for array comparative genomic hybridization on prostate cancer samples and cell lines. The most prominent observation on prostate cancer specimens was a deletion at Yp11.2 containing the TSPY tandem gene array. Out of 36 primary prostate tumors analyzed, 16 (44.4%) samples exhibited loss of TSPY gene copies. Notably, we observed association between the number of TSPY copies in the blood and the incidence of prostate cancer. Moreover, PC-3 hybrids with an intact Yp11.2 did not grow tumors in nude mice, whereas PC-3 hybrids with a deletion at Yp11.2 grew tumors in nude mice.
Vijayakumar
Sapna
S
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229-3900, USA.
Hall
Devon C
DC
Reveles
Xavier T
XT
Troyer
Dean A
DA
Thompson
Ian M
IM
Garcia
Dawn
D
Xiang
Ruihua
R
Leach
Robin J
RJ
Johnson-Pais
Teresa L
TL
Naylor
Susan L
SL
D000368:Aged; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D041322:Chromosomes, Human, Y / Q000235:genetics*; D018628:Gene Dosage; D006801:Humans; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D009367:Neoplasm Staging; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018797:Cell Cycle Proteins; C497749:TSPY1 protein, human