Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection.
A simple method for separation of different anionic and zwitterionic phospholipid classes by capillary zone electrophoresis (CZE), using indirect UV detection with adenosine monophosphate (AMP) as background electrolyte and the UV-absorbing additive, was successfully developed in this study. The separation conditions including apparent pH (pH*) of running buffer, concentration of AMP, organic solvent, applied voltage and capillary temperature were systematically optimized. The application of this method to human blood sample was also briefly examined.
Gao
Fei
F
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemistry, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Dong
Juan
J
Li
Wei
W
Wang
Tao
T
Liao
Jie
J
Liao
Yiping
Y
Liu
Huwei
H
D000249:Adenosine Monophosphate / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010743:Phospholipids / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013696:Temperature
D010743:Phospholipids; D012997:Solvents; D000249:Adenosine Monophosphate