Microscopy reveals disease control through novel effects on fungal development: a case study with an early-generation benzophenone fungicide.
The benzophenones are a new class of agricultural fungicides that demonstrate protectant, curative and eradicative/antisporulant activity against powdery mildews. The chemistry is represented in the marketplace by the fungicide metrafenone, recently introduced by BASF and discussed in the following paper. The benzophenones show no evidence of acting by previously identified biochemical mechanisms, nor do they show cross-resistance with existing fungicides. The value of microscopy in elucidating fungicide mode of action is demonstrated through identification of the effects of an early benzophenone, eBZO, on mildew development. eBZO caused profound alterations in the morphology of powdery mildews of both monocotyledons and dicotyledons, affecting multiple stages of fungal development, including spore germination, appressorial formation, penetration, surface hyphal morphology and sporogenesis. Identification of analogous effects of eBZO on sporulation in the model organism Aspergillus nidulans (Eidam) Winter provides a unique opportunity to elucidate important morphogenetic regulatory sites in the economically important obligate pathogens, the powdery mildews. Benzophenones provide a further example of the benefits of whole-organism testing in the search for novel fungicide modes of action.
Schmitt
Mark R
MR
USDA-ARS, Cereal Crops Research Unit, 501 Walnut Street, Madison, WI 53726, USA. markschmitt@wisc.edu
Carzaniga
Raffaella
R
Cotter
H Van T
HV
O'Connell
Richard
R
Hollomon
Derek
D
D001203:Ascomycota / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D001233:Aspergillus nidulans / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D001577:Benzophenones / Q000494:pharmacology*; D048690:Fruiting Bodies, Fungal / Q000187:drug effects / Q000648:ultrastructure; D005659:Fungicides, Industrial / Q000494:pharmacology*; D025301:Hyphae / Q000187:drug effects / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010944:Plants / Q000382:microbiology*; D013172:Spores, Fungal / Q000187:drug effects
D001577:Benzophenones; D005659:Fungicides, Industrial; C047723:benzophenone