Selection of RNase-resistant RNAs.
All RNA types are susceptible to ribonuclease (RNase) digestion, which might be a serious problem for several in vitro and in vivo applications. RNase resistance can be reached through chemical modifications or the selection of stable secondary structures via SELEX (systematic evolution of ligands by exponential enrichment). This chapter focuses on the selection of natural RNase-resistant RNAs, enriched by a selection process in the presence of RNase T1. Results of these investigations led to the identification of a particular structural motif, the tetraloop. Further applications could be the advised use of such motifs in order to reach higher stability of RNA molecules.
Kainz
S
S
Institut für Biochemie und Lebensmittelchemie, Abteilung Biochemie und Molekularbiologie, Universität Hamburg, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany.
Czaja
R
R
Greiner-Stöffele
T
T
Hahn
U
U
D000818:Animals; D006801:Humans; D009690:Nucleic Acid Conformation; D012313:RNA / Q000378:metabolism*; D006163:Ribonuclease T1 / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*
D012313:RNA; D012260:Ribonucleases; D006163:Ribonuclease T1