Bovine alveolar macrophage neurokinin-1 and response to substance P.
In this study bovine alveolar macrophage neurokinin-1 (NK-1) and the in vitro response to substance P (SP) exposure were investigated. Bovine alveolar macrophage membrane extracts separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted using anti-NK-1 antiserum demonstrated the presence of an approximately 60kDa band. Phagocytosis of fluorescent bioparticles by SP-exposed macrophages was 39% greater than that of non-exposed macrophages (P=0.0089). Likewise, there was 28% greater TNF production by macrophages following SP exposure compared to non-exposed controls (P=0.116). These results suggest that bovine alveolar macrophages respond to SP at least in part by enhancing phagocytosis and TNF production.
Rogers
Donna P
DP
Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, USA.
Wyatt
Carol R
CR
Walz
Paul H
PH
Drouillard
James S
JS
Mosier
Derek A
DA
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle / Q000276:immunology*; D005260:Female; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000276:immunology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D010587:Phagocytosis / Q000276:immunology; D018040:Receptors, Neurokinin-1 / Q000276:immunology*; D013373:Substance P / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D018377:Neurotransmitter Agents; D018040:Receptors, Neurokinin-1; D014409:Tumor Necrosis Factor-alpha; D013373:Substance P; D009240:N-Formylmethionine Leucyl-Phenylalanine