[Determination of cotinine in human urine with gas chromatograph-mass spectrometry].
OBJECTIVE
To establish the method of gas chromatograph-mass spectrometry (GC-MS) for the determination of the cotinine (COT) in human urine.


METHODS
The conjugated trans-3'-hydroxycotinine (THOC) and COT were hydrolyzed in human urine with beta-glucuronidase. The composition of COT was extracted with the mixture of dichloromethane and n-butyl acetate (2:1) and was separated with HP-5MS fused-silica capillary column. The GC-MS was used for determining its content.


RESULTS
The monitoring limit of this method was 0.02 microg/L. Its recovery rate was higher than 90%, Its accuracy rate was 4.30%. It was used for the determination of the cotinine in human urine in Guangzhou Biological Bank the Elderly Cohort.


CONCLUSIONS
The GC-MS method is a good microanalysis for monitoring the cotinine in human urine rapidly and accurately with little background disturbance. It has been applied in our Guangzhou Cohort Study for determining cotinine in human urine.
Cao
Min
M
Guangzhou Monitoring Centre of Occupational Health, Guangzhou 510620 China.
Zhang
Yi-lun
YL
Jiang
Chao-qiang
CQ
Lam
Tai-hing
TH
Cheng
Kar-keung
KK
Zhang
Wei-sen
WS
D003367:Cotinine / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D012680:Sensitivity and Specificity; D012907:Smoking / Q000652:urine
D003367:Cotinine