[Screening methods for detection of metallo-beta-lactamase producing gram negative rods].
Modified Hodge test and a method using a disk with imipenem plus 1000 mg of EDTA were used to determine the presence of metallo-beta-lactamase producing gram-negative rods among 166 clinical isolates from hospitals in Iaşi and Galaţi. Of 9 imipenem resistant strains found, only one Pseudomonas aeruginosa gave positive results with both tests and other two P. aeruginosa clinical isolates gave negative results with both tests. The rest of the strains (2 P. aeruginosa, 2 Acinetobacter baumanii, 1 Sphingomonas paucimobilis) did not give conclusive results. These screening methods are useful, simple and accessible to clinical laboratories. PCR is needed to confirm the presence of metallo-beta-lactamase gene in bacteria and to determine the type of the enzymes.
Mereuţă
Ana-Irina
AI
Universitatea de Medicină si Farmacie Gr.T. Popa Iaşi, Facultatea de Medicină, Disciplina de Microbiologie Medicală.
Poiati
Antonia
A
Tuchiluş
Cristina
C
Dorneanu
Olivia
O
Nistor
Silvia
S
Copăcianu
Brînduşa
B
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis* / Q000096:biosynthesis; D001769:Blood / Q000382:microbiology; D015780:Carbapenems / Q000494:pharmacology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D008403:Mass Screening; D008826:Microbial Sensitivity Tests; D015337:Multicenter Studies as Topic; D010996:Pleural Effusion / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology*; D012383:Romania; D013492:Suppuration / Q000382:microbiology; D014556:Urine / Q000382:microbiology; D001618:beta-Lactamases / Q000032:analysis* / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C082063:cphA protein, Aeromonas hydrophila