title: Binding of galectin-1 (gal-1) to the Thomsen-Friedenreich (TF) antigen on trophoblast cells and inhibition of proliferation of trophoblast tumor cells in vitro by gal-1 or an anti-TF antibody.
abstract: Galectin-1 (gal-1), a member of the mammalian beta-galactoside-binding proteins, recognizes preferentially Galbeta1-4GlcNAc sequences of several cell surface oligosaccharides. We demonstrate histochemically that the lectin recognizes appropriate glycotopes on the syncytiotrophoblast and extravillous trophoblast layer from second trimester human placenta and on BeWo chorion carcinoma cells. Gal-1 binding to BeWo cells was diminished by the Thomsen-Friedreich (TF)-disaccharide (Galbeta1-3GalNAc-) conjugated to polyacrylamide (TF-PAA). Gal-1 also inhibited BeWo cell proliferation in a concentration-dependent manner. Similar antiproliferative effects were also observed with an anti-TF monoclonal antibody (mAb, A78-G/A7). Therefore, we conclude that ligation of Galbeta1-4GlcNAc and Galbeta1-3GalNAc epitopes on BeWo cells may have regulatory effects on cell proliferation.
authors.affiliation: First Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Maistrasse 11, 80337, Munich, Germany. udo.jeschke@med.uni-muenchen.de
authors.forename: Udo
authors.initials: U
authors.lastname: Jeschke
authors.forename: Uwe
authors.initials: U
authors.lastname: Karsten
authors.forename: Irmi
authors.initials: I
authors.lastname: Wiest
authors.forename: Sandra
authors.initials: S
authors.lastname: Schulze
authors.forename: Christina
authors.initials: C
authors.lastname: Kuhn
authors.forename: Klaus
authors.initials: K
authors.lastname: Friese
authors.forename: Hermann
authors.initials: H
authors.lastname: Walzel
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D037483:Galectin 1 / Q000378:metabolism* / Q000494:pharmacology*; D006021:Glycophorins / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000494:pharmacology; D014328:Trophoblastic Neoplasms / Q000378:metabolism*; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000906:Antibodies; D015295:Antigens, Tumor-Associated, Carbohydrate; D037483:Galectin 1; D006021:Glycophorins; D007075:Immunoglobulin M; C016467:Thomsen-Friedenreich antigen; C025991:Thomsen-Friedenreich antibodies