Estrogen effects on the expression of Brx in the brain and pituitary of the mouse.
A member of the Dbl family of oncoproteins was discovered in breast cancer tissue extracts. This novel protein, designated Brx, contains an estrogen-receptor binding motif and is highly expressed in hormone-responsive breast tissue. Due to its ability to augment ligand-dependent activation of estrogen receptors, we analyzed the expression of Brx in the adult mouse brain and pituitary. Results indicated that Brx was expressed in specific regions of the brain and pituitary. Furthermore, the results indicate that differences exist in both brain and pituitary tissue of male and female mice with greater expression in the female. However, estrogen did not influence Brx expression in ovariectomized mice. The anatomical studies support a role for Brx in its association with the estrogen receptor and that Brx may be involved in neuronal and pituitary function in a sexually dimorphic manner.
Eddington
David O
DO
Program in Molecular and Cellular Biology, Uniformed Services University of Health Sciences, Bethesda, MD 20814, USA.
Baldwin
Emily L
EL
Segars
James H
JH
Wu
T J
TJ
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D020662:Guanine Nucleotide Exchange Factors; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010902:Pituitary Gland / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D012737:Sex Factors
D004967:Estrogens; D020662:Guanine Nucleotide Exchange Factors; C512843:Mcf2 protein, mouse; D011518:Proto-Oncogene Proteins; D004958:Estradiol