Increased ammonium in culture medium reduces the development of human embryos to the blastocyst stage.
The objective of this prospective study was to evaluate the effect of ammonium accumulated in sequential media and determined by enzymatic spectrophotometric method on the blastocyst development in 281 human embryos from 100 stimulated and natural in vitro fertilization (IVF) cycles. Ammonium concentration was increased in 62% of cycles and was correlated negatively with the blastocyst development after classical IVF, but not after intracytoplasmic sperm injection (ICSI).
Virant-Klun
Irma
I
Department of Obstetrics and Gynecology, Ljubljana, Slovenia. irma.virant@kclj.si
Tomazevic
Tomaz
T
Vrtacnik-Bokal
Eda
E
Vogler
Andrej
A
Krsnik
Mladen
M
Meden-Vrtovec
Helena
H
D001755:Blastocyst / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry*; D004622:Embryo, Mammalian / Q000502:physiology*; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D009994:Osmolar Concentration; D011446:Prospective Studies; D000644:Quaternary Ammonium Compounds / Q000032:analysis* / Q000494:pharmacology*; D013053:Spectrophotometry; D020554:Sperm Injections, Intracytoplasmic
D003470:Culture Media; D000644:Quaternary Ammonium Compounds