[Effect of estrogen on osteoprotegerin, osteoclast differentiation factor and macrophage colony stimulating factor mRNA expressions in ovariectomized rat bone tissue].
OBJECTIVE
To observe the effect of the estrogen on the mRNA expression of osteoprotegerin (OPG), osteoclast differentiation factor (ODF) and macrophage colony stimulating factor (M-CSF) in bone tissue of ovariectomized rats, and investigate the possible pathway of estrogen in preventing and treating postmenopausal osteoporosis. METHODS; Thirty healthy adult SD rats were randomly divided into sham operation group, ovariectomized group and estrogen-treated group. All rats were ovariectomized except those in the sham operation group. Bone density of the L3-L6 vertebra was detected 12 weeks after the operation. The total RNA were extracted from the femur to examine mRNA expression of OPG, ODF and M-CSF by real-time PCR.


RESULTS
Estrogen increased the bone density of the ovariectomized rat lumbar vertebra and up-regulated the expression of OPG, whereas down-regulated the expression of M-CSF and lowered ODF:OPG ratio.


CONCLUSIONS
The effect of estrogen in treating postmenopausal osteoporosis is closely correlated with the regulation of OPG and M-CSF expressions and ODF:OPG ratio.
Wang
Qiang
Q
Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. 46947814@126.com
Wang
Kun-zheng
KZ
Dang
Xiao-qian
XQ
Shi
Zhi-bin
ZB
Pei
Xian-wu
XW
Bai
Chuan-yi
CY
Jia
Xue-wu
XW
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D010024:Osteoporosis / Q000188:drug therapy; D053244:Osteoprotegerin / Q000096:biosynthesis* / Q000235:genetics; D010052:Ovariectomy; D053245:RANK Ligand / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D004967:Estrogens; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor