title: A method for quantitative determination of furanocoumarins in capsules and tablets of phytochemical preparations.
abstract: A method for sample preparation and analysis by high-performance liquid chromatography with UV detection (HPLC-UV) was developed for analysis of psoralen, bergapten and 5-[3-(4,5-dihydro-5,5-dimethyl-4-oxo-2-furanyl)-butoxy]-7H-furo[3-2-g][1]benzopyran-7-one in capsules and tablets employed in Brazil for certain illnesses. The linearity, accuracy, the inter- and intra-day precision of the procedure were evaluated. Analytical curves for furanocoumarins were linear in the range of 1.0-50.0 microg/ml. The recoveries of the furanocoumarins in the products analyzed were 97.3-99.5%, and the percent coefficient of variation for the quantitative analysis of the furanocoumarins in the analyses was under 5%. For inter-equipment study gas chromatography (GC) was employed.
authors.affiliation: Curso de Química, Universidade Estadual de Mato Grosso do Sul, Dourados/MS, Brazil. claudia@uems.br
authors.forename: Claudia Andréa Lima
authors.initials: CA
authors.lastname: Cardoso
authors.forename: Adriana Elias
authors.initials: AE
authors.lastname: Pires
authors.forename: Neli Kika
authors.initials: NK
authors.lastname: Honda
mesh_terms: D000078223:5-Methoxypsoralen; D001578:Benzopyrans / Q000032:analysis*; D002214:Capsules / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005363:Ficusin / Q000032:analysis*; D011564:Furocoumarins / Q000032:analysis*; D008730:Methoxsalen / Q000031:analogs & derivatives* / Q000032:analysis; D017319:Photosensitizing Agents / Q000032:analysis*; D028321:Plant Preparations / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets / Q000737:chemistry
chemical_list: D001578:Benzopyrans; D002214:Capsules; D011564:Furocoumarins; D017319:Photosensitizing Agents; D028321:Plant Preparations; D013607:Tablets; D000078223:5-Methoxypsoralen; D005363:Ficusin; D008730:Methoxsalen