title: [Morphological changes of ECV304 cells infected by herpes simplex virus type 2].
abstract: OBJECTIVE
  To observe the pathological changes and morphological alterations of ECV304 cells after the infection by herpes simplex virus type 2 (HSV-2) in vitro.
  
  
  METHODS
  Passaged ECV304 cells were infected with HSV-2, TCID50 and morphological changes were observed by optical microscopy and tissue staining.
  
  
  RESULTS
  One day after HSV-2 infection, swelling, rounding, and increase of thickened cytoplasmic granules occurred in the ECV304 cells, and on day 2, cell fusion was observed with weakened nuclear staining.
  
  
  CONCLUSIONS
  ECV304 cells mostly undergo necrosis after HSV-2 infection without obvious evidence of cell apoptosis.
authors.affiliation: Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Hai-quan
authors.initials: HQ
authors.lastname: Zhao
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Ma
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Zhang
authors.forename: Xiao-yang
authors.initials: XY
authors.lastname: Mo
authors.forename: Chang-wen
authors.initials: CW
authors.lastname: Ke
authors.forename: Huan-ying
authors.initials: HY
authors.lastname: Zheng
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000473:pathology* / Q000821:virology*; D006566:Herpesviridae Infections / Q000473:pathology*; D018258:Herpesvirus 2, Human; D006801:Humans; D009336:Necrosis; D014471:Umbilical Veins / Q000473:pathology