[The application of co-culture system on the in vitro development of bovine somatic nuclear transferred embryos].
To establish a co-culture system of nuclear transferred embryos in bovine, effects of co-culture cell types, passages and cryopreservation as well as addition of BFF or FBS were investigated. The results showed that embryos co-cultured with oviductal epithelial cell and granulosa cell achieved significantly higher blastocyst rate compared with the control group (P < 0.05) and co-cultured with oviductal epithelial cell had more embryo cell number than those with granulosa cell. Passages of co-culture cells significantly affected the blastocyst rate and embryo cell number (P < 0.05), and cryopreservation decreased the blastocyst rate and embryo cell number remarkably. Supplemention of BFF increased blastocyste rate significantly (P < 0.05). In conclusion, co-cultured with fresh primary oviductal epithelial cell along with addition of 10% BFF in SOFaa could improve development of nuclear transferred bovine embryo in vitro.
Wu
Yue-Hong
YH
Bioengineering Institute, Northwest Agricultural & Forstry University, Yangling 712100, China.
An
Zhi-Xing
ZX
Zhang
Yong
Y
Li
Xiang-Chen
XC
He
Yu-Long
YL
Liu
Feng-Jun
FJ
D000818:Animals; D002417:Cattle; D065150:Cellular Reprogramming; D019976:Cloning, Organism / Q000379:methods*; D018920:Coculture Techniques; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects; D047108:Embryonic Development; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects; D053652:Nuclear Transfer Techniques
D003470:Culture Media