title: Solution structures of spinach acyl carrier protein with decanoate and stearate.
abstract: Acyl carrier protein (ACP) is a cofactor in a variety of biosynthetic pathways, including fatty acid metabolism. Thus, it is of interest to determine structures of physiologically relevant ACP-fatty acid complexes. We report here the NMR solution structures of spinach ACP with decanoate (10:0-ACP) and stearate (18:0-ACP) attached to the 4'-phosphopantetheine prosthetic group. The protein in the fatty acid complexes adopts a single conformer, unlike apo- and holo-ACP, which interconvert in solution between two major conformers. The protein component of both 10:0- and 18:0-ACP adopts the four-helix bundle topology characteristic of ACP, and a fatty acid binding cavity was identified in both structures. Portions of the protein close in space to the fatty acid and the 4'-phosphopantetheine were identified using filtered/edited NOESY experiments. A docking protocol was used to generate protein structures containing bound fatty acid for 10:0- and 18:0-ACP. In both cases, the predominant structure contained fatty acid bound down the center of the helical bundle, in agreement with the location of the fatty acid binding pockets. These structures demonstrate the conformational flexibility of spinach ACP and suggest how the protein changes to accommodate its myriad binding partners.
authors.affiliation: Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706-1549, USA.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Zornetzer
authors.forename: Brian G
authors.initials: BG
authors.lastname: Fox
authors.forename: John L
authors.initials: JL
authors.lastname: Markley
mesh_terms: D000213:Acyl Carrier Protein / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D003651:Decanoates / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010204:Pantetheine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D020836:Protein Structure, Quaternary; D012694:Serine / Q000378:metabolism; D012996:Solutions; D018724:Spinacia oleracea / Q000737:chemistry*; D013228:Stearates / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000213:Acyl Carrier Protein; D003651:Decanoates; D012996:Solutions; D013228:Stearates; D012694:Serine; D010204:Pantetheine; C003129:4'-phosphopantetheine