[Analysis of SNPs in exons 2 and 3 of the leptin gene in wild boar, swine and hybrid swine].
Leptin is a 167-amino acid hormone mainly expressed in the fat tissue. It serves as an important regulator of energy uptake and consumption through action on the hypothalamus. Herein single nucleotide polymorphisms (SNPs) were detected in exons 2 and 3 of the Leptin gene in wild boar, swine and half-bred swine by PCR-SSCP. Cloning and sequencing of homozygous fragments revealed the following variations in the coding region: a silent T to C transversion at nucleotide 214; CG to GC at nucleotides 364 and 365, which converts Arg to Ala; a silent G to A transversion at nucleotide 426; a T insertion at nucleotide 451 resulting in frameshift; and a G to T transversion at nucleotide 462.
Li
Jing-Fen
JF
Animal Science Technical College, Northeast Agricultural University, Harbin 150030, China. chenxi8689@163.com
Yu
Hao
H
Liu
Di
D
D019943:Amino Acid Substitution; D000818:Animals; D002678:Chimera; D005091:Exons; D016368:Frameshift Mutation; D020738:Leptin / Q000235:genetics*; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA / Q000379:methods; D034421:Sus scrofa; D013552:Swine
D020738:Leptin