[A study on K469E polymorphism of ICAM1 gene and ICAM1 plasma level in patients with coronary heart disease].
OBJECTIVE
To study the linkage between K469E polymorphism of intercellular adhesion molecule 1(ICAM1) gene with ICAM1 plasma level and coronary heart disease (CHD) in Han population of China.


METHODS
One hundred and sixty-four controls without CHD and 160 patients with CHD were enrolled in our study. By nested PCR with allele-specific oligonucleotide primers, all patients and controls were genotyped for the ICAM1 polymorphism. And the ICAM1 plasma level was measured by ELISA.


RESULTS
In the patients with CHD, both K allele frequency and the plasma level of ICAM1 were higher than those in control (P<0.05). The individual with K allele had higher plasma level of ICAM1 than that without K allele (344.34+/-128.59 microg/L vs 303.54+/-108.74 microg/L, P=0.008). K allele enhanced the risk of CHD (P<0.01, OR=2.158, 95%CI: 1.250-3.727). There was the K allele cooperation with smoking in influencing the risk of CHD.


CONCLUSIONS
There is the polymorphism of ICAM1 K469E gene in Han population of China, and the K allele may be a genetic factor influencing the risk of CHD.
Lu
Fang-hong
FH
Department of Cardiovascular Disease, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, Shandong, 250062 P.R.China. lufanghongming@tom.com
Shang
Qing
Q
Wen
Pei-e
PE
Su
Guo-hai
GH
Wu
Jian-mei
JM
Tian
Qi
Q
Zhao
Ying-xin
YX
D002681:China / Q000208:ethnology; D003327:Coronary Disease / Q000097:blood* / Q000235:genetics; D005787:Gene Frequency; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood / Q000235:genetics*; D011110:Polymorphism, Genetic; D011188:Potassium / Q000037:antagonists & inhibitors
D018799:Intercellular Adhesion Molecule-1; D011188:Potassium