Proteomic analysis of rat aorta during atherosclerosis induced by high cholesterol diet and injection of vitamin D3.
1. Atherosclerosis (AS) in rats displays important clinical similarities to human AS. 2. After the experimental model of AS in rat was established and using a proteomic approach, we compared the protein profiling of aorta tissues from healthy and AS rats. 3. Using two-dimensional electrophoresis (2-DE), over 1878 protein species were separated; among them, 1239 protein spots were matched between different gels with average matching rate of approximately 66%. Gel analysis and protein characterization have identified 58 protein spots whose abundance is significantly altered in AS rats. 4. By using matrix-associated laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) and NCBInr database, 46 proteins were successfully identified. Among them, 18 proteins were of increased abundance in diseased tissues including a group of oxidization-related enzymes such as peroxiredoxin2 and NADH dehydrogenase Fe-S protein 6, components of inflammatory pathways such as lamin A, while 28 proteins were of decreased abundance in the diseased state, including CaM-KII inhibitory protein, transferring, fructose-bisphosphate aldolase. 5. We believe that these results would give insights into the cellular and molecular mechanisms involved in AS development and might lead to the discovery of novel diagnostic markers and new therapeutic opportunities.
Almofti
Mohamad Radwan
MR
Center of Proteome Research & Department of Chemistry, Fudan University, Shanghai, China.
Huang
Zhenyu
Z
Yang
Pengyuan
P
Rui
Yaocheng
Y
Yang
Pengyuan
P
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D050197:Atherosclerosis / Q000378:metabolism*; D015415:Biomarkers; D002762:Cholecalciferol / Q000494:pharmacology*; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D030541:Databases, Genetic; D004036:Diet, Atherogenic; D015180:Electrophoresis, Gel, Two-Dimensional; D007202:Indicators and Reagents; D008297:Male; D010449:Peptide Mapping; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014815:Vitamins / Q000494:pharmacology*
D015415:Biomarkers; D002791:Cholesterol, Dietary; D007202:Indicators and Reagents; D014815:Vitamins; D002762:Cholecalciferol