title: Functional display of Bacillus thuringiensis Cry1Ac toxin on T7 phage.
abstract: The Cry1Ac toxin from Bacillus thuringiensis was displayed on the surface of T7 phage. The cry1Ac gene was fused to the C-terminal end of T7-10B capsid protein and displayed on the surface of T7 phage as revealed by Western blot analysis of the purified phage particles. The T7-Cry1Ac phages retained toxicity against Manduca sexta larvae. We demonstrated that the T7-Cry1Ac phage interacts with Cry1Ac receptors present in M. sexta BBMV either in solution or in overlay binding assays.
authors.affiliation: Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
authors.forename: Sabino
authors.initials: S
authors.lastname: Pacheco
authors.forename: Isabel
authors.initials: I
authors.lastname: Gómez
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sato
authors.forename: Alejandra
authors.initials: A
authors.lastname: Bravo
authors.forename: Mario
authors.initials: M
authors.lastname: Soberón
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000502:physiology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017123:Bacteriophage T7 / Q000235:genetics*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D007814:Larva; D018402:Manduca / Q000378:metabolism; D010572:Pest Control, Biological / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins