[Gamma-aminobutyric acid B receptor regulates the expression of hydrogen sulfide/cystathionine-beta-synthase system in recurrent febrile seizures].
OBJECTIVE
Febrile seizure (FS) is one of the most common seizure types in children. Our previous studies have demonstrated that both gamma-aminobutyric acid B receptor (GABABR) and hydrogen sulfide (H2S) are involved in the pathogenesis of FS. This study was designed to explore the effect of GABABR on H2S/cystathionine-beta-synthase (CBS) system in recurrent FS.


METHODS
Sixty-four Sprague-Dawley rats aged 21 days were randomly assigned into four groups: Control (37 degrees C water bath exposure), FS, FS+baclofen (GABABR excitomotor), and FS+phaclofen (GABABR inhibitor) groups (n=16 each). FS was induced by warm water bath exposure (45.2 degrees C, once every 2 days, 10 times in total. The plasma level of H2S was detected by the spectrophotometer. The expression of CBS mRNA was examined by in situ hybridization. The expressions of CBS protein was observed by immunohistochemistry.


RESULTS
The plasma level of H2S increased in the FS+baclofen group (427.45 +/- 15.91 micromol/L) but decreased in the FS+phaclofen group (189.72 +/- 21.53 micromol/L) compared with that in the FS group (362.14 +/- 19.71 micromol/L). The expressions of CBS mRNA and protein were up-regulated in the FS+baclofen group but were down-regulated in the FS+phaclofen group compared with those in the FS group.


CONCLUSIONS
GABABR modulated the expression of H2S/CBS system in recurrent FS.
Han
Ying
Y
Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.
Qin
Jiong
J
Bu
Ding-Fang
DF
Chang
Xing-Zhi
XZ
Yang
Zhi-Xian
ZX
Du
Jun-Bao
JB
D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D003541:Cystathionine beta-Synthase / Q000235:genetics / Q000502:physiology*; D006862:Hydrogen Sulfide / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000502:physiology*; D012008:Recurrence; D003294:Seizures, Febrile / Q000378:metabolism*
D018080:Receptors, GABA-B; D003541:Cystathionine beta-Synthase; D001418:Baclofen; D006862:Hydrogen Sulfide