Cell growth and shikonin production of Arnebia euchroma in a periodically submerged airlift bioreactor.
Arnebia euchroma was grown in a 2-l periodically submerged, airlift bioreactor (PSAB) in which the non-submerged (immobilization culture) and submerged (suspension culture) operations were controlled automatically. PSAB had advantages in improving cell growth, shikonin content, shikonin production and cell aggregation compared with suspension culture. Under the optimal submerged/non-submerged period of 10 min/15 h, the shikonin content (4.6%, w/w) and, cell dry mass (16.8 g/l) were 229 and 26% higher than those in suspension culture.
Ge
Feng
F
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P. O. Box 353, Beijing 100080, China. gefeng79@yahoo.com.cn
Yuan
Xiaofan
X
Wang
Xiaodong
X
Zhao
Bing
B
Wang
Yuchun
Y
D018533:Biomass; D019149:Bioreactors; D028082:Boraginaceae / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D002455:Cell Division; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D009285:Naphthoquinones / Q000378:metabolism*; D013997:Time Factors
D009285:Naphthoquinones; C016101:shikonin