Effects of fibrin sealant on single-layer uterine incision closure in the New Zealand white rabbit.
OBJECTIVE
To determine if the addition of fibrin sealant to incision closure in a rabbit uterine horn myomectomy model affects adhesion formation or strength of incision closure.


METHODS
Prospective randomized controlled trial.


METHODS
Academic research center.


METHODS
New Zealand white female rabbits.


METHODS
A pilot study revealed that the time interval for maximal uterine incision healing was eight weeks. Thirty New Zealand white rabbits underwent a 1-cm standardized myotomy incision on both uterine horns. A single interrupted stitch of 3-0 polygalactin suture was placed to reapproximate each incision. Fibrin sealant was then applied to one of each rabbit's horns (randomized). After eight weeks, the rabbits were killed, and the strength of myotomy closure was determined by measurement of maximal burst pressure for each uterine horn. Adhesion presence was recorded.


METHODS
Uterine burst pressure, adhesion presence.


RESULTS
The mean burst pressure was 267.8 (+/-75.8) mm Hg in the suture only group and 247.8 (+/-92.3) mm Hg in the suture and fibrin sealant group. There was no statistical difference in the presence of adhesions.


CONCLUSIONS
Fibrin sealant did not strengthen myotomy repair nor did it reduce postoperative adhesion formation. There is no apparent advantage to fibrin sealant in this myomectomy model.
Peacock
Kelly E
KE
Department of Obstetrics and Gynecology, Carolinas Medical Center, Charlotte, North Carolina, USA.
Hurst
Bradley S
BS
Marshburn
Paul B
PB
Matthews
Michelle L
ML
D000268:Adhesiveness / Q000187:drug effects; D000818:Animals; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000008:administration & dosage*; D013509:Gynecologic Surgical Procedures / Q000009:adverse effects / Q000379:methods*; D010535:Laparoscopy / Q000009:adverse effects / Q000379:methods*; D010865:Pilot Projects; D011183:Postoperative Complications / Q000517:prevention & control; D011817:Rabbits; D013718:Tensile Strength; D014014:Tissue Adhesives / Q000008:administration & dosage; D014599:Uterus / Q000187:drug effects* / Q000503:physiopathology / Q000601:surgery*; D014945:Wound Healing / Q000187:drug effects*
D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives