Preimplantation genetic diagnosis of leukocyte adhesion deficiency type I.
OBJECTIVE
To describe the application of preimplantion genetic diagnosis (PF to a carrier couple for leukocyte adhesion deficiency type I disease (LAD-1), to achieve a healthy pregnancy.


METHODS
Case report.


METHODS
Reproductive center and university hospital.


METHODS
A couple in which both partners were carriers for LAD-1; the female partner carried a G400A substitution in exon 4, and the male partner carried a C562T substitution in exon 5 in the CD18 gene.


METHODS
Day-3 cleavage-stage biopsy after standard in vitro fertilization (IVF) and genetic analysis of blastomeres for two mutations, along with a marker from chromosome 21.


METHODS
Birth of a child unaffected with LAD-1.


RESULTS
Fifteen oocytes were retrieved, of which 10 were fertilized; eight embryos were suitable for embryo biopsy. After genetic analysis, three embryos were found to be unaffected. According to embryo morphology, two embryos were transferred, resulting in the birth of an unaffected child.


CONCLUSIONS
This is the first report of preimplantion genetic diagnosis for LAD-1. The successful birth of a healthy child provides evidence that for carrier couples of diseases for which traditional prenatal diagnosis and the decision of whether to terminate a pregnancy might not be acceptable, the application of PGD provides an alternative.
Lorusso
Filomenamila
F
Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
Kong
Dewen
D
Jalil
Ahmad Kamal Abdul
AK
Sylvestre
Camille
C
Tan
Seang Lin
SL
Ao
Asangla
A
D000225:Adenine; D000328:Adult; D003596:Cytosine; D004624:Embryo Transfer; D005091:Exons; D005260:Female; D005307:Fertilization in Vitro; D006147:Guanine; D006801:Humans; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000145:classification / Q000235:genetics*; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis; D013941:Thymine
D006147:Guanine; D003596:Cytosine; D000225:Adenine; D013941:Thymine