title: Universal primers for detection of common bacterial pathogens causing prosthetic joint infection.
abstract: The diagnosis of low grade prosthetic joint infection is difficult and time consuming. Nested-PCR for universal bacterial DNA segments detection of "orthopaedic" bacteria was tested in a laboratory setting. This method is based on amplification of the 16S bacterial ribosomal RNA coding sequences. 11 species of the most frequent bacterial pathogens (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus agalactiae, Enterococcus faecium, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens) involved in prosthetic joint infections were studied. All could be detected rapidly and sensitively by this method.
authors.affiliation: Institute of Microbiology, Palacký University, Teaching Hospital, Olomouc, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Sauer
authors.forename: Jirí
authors.initials: J
authors.lastname: Gallo
authors.forename: Michaela
authors.initials: M
authors.lastname: Kesselová
authors.forename: Milan
authors.initials: M
authors.lastname: Kolár
authors.forename: Dagmar
authors.initials: D
authors.lastname: Koukalová
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000382:microbiology*; D017931:DNA Primers; D006801:Humans; D007595:Joint Prosthesis; D007596:Joints / Q000382:microbiology; D016133:Polymerase Chain Reaction; D016459:Prosthesis-Related Infections / Q000382:microbiology*
chemical_list: D017931:DNA Primers