title: The ABCG5 polymorphism contributes to individual responses to dietary cholesterol and carotenoids in eggs.
abstract: The ATP binding cassette G5 (ABCG5) polymorphisms have been postulated to play a role in the response to dietary cholesterol. The objective of this study was to examine the contribution of the ABCG5 polymorphism on the plasma response to consumption of cholesterol and carotenoids from eggs. For this purpose, genotyping was conducted for 40 men and 51 premenopausal women who were randomly assigned to consume an egg (EGG, 640 mg/d additional dietary cholesterol and 600 microg lutein+ zeaxanthin) or placebo (SUB, 0 mg/d cholesterol, 0 microg lutein + zeaxanthin) diet for 30 d. The two arms of the dietary intervention were separated by a 3-wk washout period. Plasma concentrations of total cholesterol, LDL cholesterol (LDL-C), and HDL cholesterol were determined. Because eggs are an excellent source of lutein and zeaxanthin, the plasma levels of these carotenoids were also measured in a subset of subjects to determine whether the response to carotenoid intake was similar to that seen for dietary cholesterol and to evaluate the contribution of ABCG5 polymorphism to both responses. Individuals possessing the C/C genotype experienced a greater increase in both LDL-C (P < 0.05) and a trend for lutein (P = 0.08) during the EGG period compared with those individuals with the C/G (heterozygote) or G/G genotypes (homozygotes). These results, although obtained from a small number of subjects, suggest that the ABCG5 polymorphism may play a role in the plasma response to dietary cholesterol and carotenoids.
authors.affiliation: Department of Nutritional Sciences, University of Connecticut, Storrs, CT, USA.
authors.forename: Kristin L
authors.initials: KL
authors.lastname: Herron
authors.forename: Mary M
authors.initials: MM
authors.lastname: McGrane
authors.forename: David
authors.initials: D
authors.lastname: Waters
authors.forename: Ingrid E
authors.initials: IE
authors.lastname: Lofgren
authors.forename: Richard M
authors.initials: RM
authors.lastname: Clark
authors.forename: Jose M
authors.initials: JM
authors.lastname: Ordovas
authors.forename: Maria Luz
authors.initials: ML
authors.lastname: Fernandez
mesh_terms: D000071018:ATP Binding Cassette Transporter, Subfamily G, Member 5; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000328:Adult; D002338:Carotenoids / Q000494:pharmacology*; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004531:Eggs; D005260:Female; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008074:Lipoproteins / Q000235:genetics*; D014975:Lutein / Q000494:pharmacology; D008297:Male; D011110:Polymorphism, Genetic
chemical_list: C416945:ABCG5 protein, human; D000071018:ATP Binding Cassette Transporter, Subfamily G, Member 5; D018528:ATP-Binding Cassette Transporters; D002791:Cholesterol, Dietary; D008074:Lipoproteins; D002338:Carotenoids; D004247:DNA; D014975:Lutein