Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099.
This study reports the production of xylanolytic and cellulolytic enzymes by a thermophilic fungal isolate Myceliophthora sp. using a cheap medium containing rice straw and chemically defined basal medium under solid-state culture. A combination of one factor at a time approach followed by response surface methodology using Box-Behnken design of experiments resulted in 2.5, 1.25, 1.28 and 4.23 fold increase in xylanase, endoglucanase, beta-glucosidase and FPase activity, respectively. The zymograms developed against IEF gels showed that multiple isoforms of xylanase (5), endoglucanase (4) and beta-glucosidase (2) were produced under optimized culture conditions. Moreover, thiol containing serine proteases produced during the growth of the culture had no role in the post-translational modification of these xylanases.
Badhan
A K
AK
Department of Microbiology, Guru Nanak Dev University, Amritsar 143 005, Punjab, India.
Chadha
B S
BS
Kaur
Jatinder
J
Saini
H S
HS
Bhat
M K
MK
D001203:Ascomycota / Q000201:enzymology*; D002480:Cellulase / Q000378:metabolism*; D003470:Culture Media; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D006358:Hot Temperature; D001617:beta-Glucosidase / Q000378:metabolism*
D003470:Culture Media; D001617:beta-Glucosidase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases