Development and validation of a RP-HPLC method for quantification of isoflavone aglycones in hydrolyzed soy dry extracts.
Isoflavones are widely used as an alternative treatment to hormone replacement therapy and also for prevention of several chronic diseases, including cancers. Genistein, daidzein and glycitein are the most abundant isoflavone aglycones found in soy extracts, where they also occur as glycosides. This paper describes the development and validation of an isocratic reversed-phase HPLC (RP-HPLC) method for the analysis of isoflavone aglycones, released after acid hydrolysis of soy dry extracts, used as pharmaceutical raw material. The quantification was carried out in a C(18) endcapped column, using a mobile phase composed of 0.1% acetic acid and methanol (52:48), at a flow rate of 1.0 ml/min and diode array detection (DAD) at 254 nm. The method showed to be linear (r(2)>0.99), precise (R.S.D.<2%), accurate (recovery of 98.88% for daidzein and 98.12% for genistein), robust and specific.
César
Isabela da Costa
Ida C
Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil. isaccesar@bol.com.br
Braga
Fernão Castro
FC
Soares
Cristina Duarte Vianna
CD
Nunan
Elzíria de Aguiar
Ede A
Pianetti
Gerson Antônio
GA
Condessa
Felipe Antonacci
FA
Barbosa
Thiago Assis F
TA
Campos
Ligia Maria Moreira
LM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006868:Hydrolysis; D007529:Isoflavones / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry*
D007529:Isoflavones; D010936:Plant Extracts