title: Simultaneous determination of pancuronium, vecuronium and their related compounds using LC-ESI-MS.
abstract: A simultaneous determination method of quaternary amino steroidal muscle relaxants, pancuronium (PAN), vecuronium (VEC), and 17-monodesacetyl pancuronium (17-OH-PAN), 3,17-bisdesacetyl pancuronium (3,17-OH-PAN), 3-monodesacetyl vecuronium (3-OH-VEC), 3,17-bisdesacetyl vecuronium (3,17-OH-VEC) in human serum was developed using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The weak cation exchange cartridge was useful for the extraction of these compounds. Under optimized LC-ESI-MS conditions, these compounds were almost fully separated within 6.5 min. Linear responses over the concentration range 0.25-50.0 ng/mL were demonstrated for each compound. The developed method successfully detected VEC, 3-OH-VEC and 3,17-OH-VEC in serum intravenously administered with VEC. The level of 3-OH-VEC was higher than other compounds. This suggested that 3-OH-VEC was useful as a forensic probe in VEC administration.
authors.affiliation: Division of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Usui
authors.forename: Takanori
authors.initials: T
authors.lastname: Hishinuma
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Toshihide
authors.initials: T
authors.lastname: Saga
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Wagatsuma
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Hoshi
authors.forename: Naoki
authors.initials: N
authors.lastname: Tachiiri
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Miura
authors.forename: Junichi
authors.initials: J
authors.lastname: Goto
mesh_terms: D002853:Chromatography, Liquid; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D003473:Neuromuscular Nondepolarizing Agents / Q000097:blood*; D010197:Pancuronium / Q000031:analogs & derivatives / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization; D014673:Vecuronium Bromide / Q000031:analogs & derivatives / Q000097:blood*
chemical_list: C515389:17-(monodesacetyl)pancuronium; D003473:Neuromuscular Nondepolarizing Agents; C056450:(dideacetoxy)vecuronium; C056447:(dideacetoxy)pancuronium; D014673:Vecuronium Bromide; C056449:3-(deacetoxy)vecuronium; D010197:Pancuronium