[Effect of feeding pre-L-methionine on high-cell-density fermentation for S-adenosyl-L-methionine production].
The yield of S-adenosyl-L-methionine (SAM) by saccharomyces cerevisiae fermentation was affected by the strategy of feeding L-methionine. The effects that feeding strategies and the amount of precursor L-methionine had on the production of SAM by saccharomyces cerevisiae G14 were investigated. The results showed that feeding L-methionine could obviously improve the accumulation of SAM, and both the biomass and SAM yield relied heavily on different feeding strategies. In our work, it was found that total amount of L-methionine added should be no less than 0.7g per 10 grams of dry cell weight. Five different feeding strategies had been investigated in our experiment, and such comparison indicated that favorable results could be achieved as the biomass reached the status of high cell density (120g/L). If 9 grams of the precursor L-methionine was introduced once and for all, the accumulation of SAM reached maximum of 4.31g/L at the 18th hour after addition; if the precursor amino acid was fed at a rate of 2g/h in 5 h, maximum yield of 4.98g/L was achieved at the 28th hour after feeding. Thus high cell density fermentation can be successfully applied to SAM production by Saccharomyces cerevisiae with the consequence of over 130g/L of biomass gained using the above two strategies.
Liu
Pei-Yi
PY
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Dong
Han-Zhu
HZ
Tan
Tian-Wei
TW
D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D005285:Fermentation; D008715:Methionine / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D012436:S-Adenosylmethionine; D008715:Methionine