title: Stability of barley and malt lipid transfer protein 1 (LTP1) toward heating and reducing agents: relationships with the brewing process.
abstract: Barley lipid transfer protein (LTP1) is a heat-stable and protease-resistant albumin that concentrates in beer, where it participates in the formation and stability of beer foam. Whereas the barley LTP1 does not display any foaming properties, the corresponding beer protein is surface-active. Such an improvement is related to glycation by Maillard reactions on malting, acylation on mashing, and structural unfolding on brewing. The structural stability of purified barley and glycated malt LTP1 toward heating has been analyzed. Whatever the modification, lipid adduction or glycation, barley LTP1s are highly stable proteins that resisted temperatures up to 100 degrees C. Unfolding of LTP1 occurred only when heating was conducted in the presence of a reducing agent. In the presence of sodium sulfite, the lipid-adducted barley and malt LTP1 displayed higher heat stability than the nonadducted protein. Glycation had no or weak effect on heat-induced unfolding. Finally, it was shown that unfolding occurred on wort boiling before fermentation and that the reducing conditions are provided by malt extract.
authors.affiliation: Unité de Recherche Biopolymères, Interactions, Assemblages, INRA, rue de la Géraudière, B.P. 71627, 44316 Nantes Cedex 03, France.
authors.forename: Ludivine
authors.initials: L
authors.lastname: Perrocheau
authors.forename: Benedicte
authors.initials: B
authors.lastname: Bakan
authors.forename: Patrick
authors.initials: P
authors.lastname: Boivin
authors.forename: Didier
authors.initials: D
authors.lastname: Marion
mesh_terms: D001515:Beer / Q000032:analysis*; D002352:Carrier Proteins / Q000737:chemistry*; D004355:Drug Stability; D002523:Edible Grain / Q000737:chemistry*; D050556:Fatty Acid-Binding Proteins; D005511:Food Handling / Q000379:methods; D006031:Glycosylation; D001467:Hordeum / Q000737:chemistry*; D006358:Hot Temperature; D015416:Maillard Reaction; D019163:Reducing Agents / Q000494:pharmacology*
chemical_list: D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; C422456:Ltp1 protein, barley; D019163:Reducing Agents