[A two-dimensional electrophoresis protocol suitable for proteomic study of rice leaves].
A 2-DE (two-dimensional electrophoresis) protocol suited for the separation of proteins from rice leaves was established. Protein extraction, quantitative loading of samples and concentrations of the gel were modified and improved to minimize markedly the interference by leaf pigments and other non-protein substances, thus resulting in satisfactory separation results.
Wang
Yu-Qi
YQ
Lab of Molecular Plant Physiology, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
Peng
Xin-Xiang
XX
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D012275:Oryza / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results
D010940:Plant Proteins