title: Global gene expression profiling of Bovine immature B cells using serial analysis of gene expression.
abstract: The Peyer's patches of the small intestine are important sites of antigen processing. The follicles of the Peyer's patches receive the antigen transported by the M cells at the mucosal surface and then play a major role in development of both protective humoral and mucosal immune responses. Serial analysis of gene expression (SAGE) was employed to derive the global gene expression profile of B lymphocytes isolated from the IPPF. Analysis of the SAGE data revealed the identity of genes and the level to which they are expressed by IPPF B lymphocytes. This analysis indicated that they were metabolically active and that the transcripts encoding proteins necessary for a response to antigen presentation were expressed. These transcripts included the B cell receptor components CD76a and b (Ig-alpha and Ig-beta) and accessory c-Src family kinases Lyn and Blk. Furthermore, many of the positive and negative regulators, and accessory proteins that are necessary for B cell antigen receptor signaling were identified. Also present were transcripts encoding interleukins and their cognate receptors. Overall, this SAGE analysis yielded a global picture of gene expression in IPPF B lymphocytes and provides a starting point for the comparison of gene expression in further functional studies.
authors.affiliation: Virus and Prion Diseases of Livestock Research Unit, USDA, ARS, National Animal Disease Center, Ames, Iowa 50010, USA. jneill@nadc.ars.usda.gov
authors.forename: John D
authors.initials: JD
authors.lastname: Neill
authors.forename: Julia F
authors.initials: JF
authors.lastname: Ridpath
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Liebler-Tenorio
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D002417:Cattle / Q000276:immunology*; D020869:Gene Expression Profiling; D007378:Interleukins / Q000096:biosynthesis / Q000235:genetics; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA; D019061:src-Family Kinases / Q000096:biosynthesis / Q000235:genetics
chemical_list: D015703:Antigens, CD; C063411:CD76 antigen; D007378:Interleukins; D018123:Receptors, Interleukin; D019061:src-Family Kinases