Proteomic pattern-based analyses of light responses in Arabidopsis thaliana wild-type and photoreceptor mutants.
Light critically affects the physiology of plants. Using two-dimensional gel electrophoresis, we used a proteomics approach to analyze the responses of Arabidopsis thaliana to red (660 nm), far-red (730 nm) and blue (450 nm) light, which are utilized by type II and type I phytochromes, and blue light receptors, respectively. Under specific light treatments, the proteomic profiles of 49 protein spots exhibited over 1.8-fold difference in protein abundance, significant at p <0.05. Most of these proteins were metabolic enzymes, indicating metabolic changes induced by light of specific wavelengths. The differentially-expressed proteins formed seven clusters, reflecting co-regulation. We used the 49 differentially-regulated proteins as molecular markers for plant responses to light, and by developing a procedure that calculates the Pearson correlation distance of cluster-to-cluster similarity in expression changes, we assessed the proteome-based relatedness of light responses for wild-type and phytochrome mutant plants. Overall, this assessment was consistent with the known physiological responses of plants to light. However, we also observed a number of novel responses at the proteomic level, which were not predicted from known physiological changes.
Kim
Dong Su
DS
Division of Molecular and Life Sciences and Systems Bio-Dynamics Research Center, POSTECH, Hyoja-dong, Pohang, Kyungbuk 790-784, Republic of Korea.
Cho
Dae Shik
DS
Park
Won-Man
WM
Na
Hyung Jin
HJ
Nam
Hong Gil
HG
D017360:Arabidopsis / Q000378:metabolism / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000096:biosynthesis*; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D008027:Light; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics* / Q000502:physiology; D010834:Phytochrome / Q000235:genetics / Q000502:physiology; D020543:Proteome / Q000096:biosynthesis*; D015398:Signal Transduction
D029681:Arabidopsis Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D020543:Proteome; D010834:Phytochrome