Picoliter-volume aqueous droplets in oil: electrochemical detection and yeast cell electroporation.
An electrochemical detection method was introduced for aqueous droplet analysis in oil phase of microfluidic devices. This method is based on the electrochemical signal difference between aqueous and oil. Applying a low alternating current (AC) voltage to a couple of Au microelectrodes, this method can offer size information and ion concentration range from 0.02 mmol/L to 1 mol/L of tens of picoliter to nanoliter aqueous droplets. Alternatively, applying a relative high AC voltage (18 Vpp) at a frequency of 1 kHz leads to electroporation of yeast cells encapsulated into picoliter droplets. We believe that this simple technique is useful for a number of aqueous droplet-based chemical and biological analyses as well as cell electroporation.
Luo
Chunxiong
C
Center for Microfluidic and Nanotechnology, School of Physics, Peking University, Beijing, China.
Yang
Xiaojing
X
Fu
Qiang
Q
Sun
Manhui
M
Ouyang
Qi
Q
Chen
Yong
Y
Ji
Hang
H
D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D018274:Electroporation; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D009821:Oils; D012441:Saccharomyces cerevisiae; D014867:Water
D009821:Oils; D014867:Water