Evidence for folate-salvage reactions in plants.
Folates in vivo undergo oxidative cleavage, giving pterin and p-aminobenzoylglutamate (pABAGlu) moieties. These breakdown products are excreted in animals, but their fate is unclear in microorganisms and unknown in plants. As indirect evidence from this and previous studies strongly suggests that plants can have high folate-breakdown rates (approximately 10% per day), salvage of the cleavage products seems likely. Four sets of observations support this possibility. First, cleavage products do not normally accumulate: pools of pABAGlu (including its polyglutamyl forms) are equivalent to, at most, 4-14% of typical total folate pools in Arabidopsis thaliana, Lycopersicon esculentum and Pisum sativum tissues. Pools of the pterin oxidation end-product pterin-6-carboxylate are, likewise, fairly small (3-37%) relative to total folate pools. Second, little pABAGlu built up in A. thaliana plantlets when net folate breakdown was induced by blocking folate synthesis with sulfanilamide. Third, A. thaliana and L. esculentum tissues readily converted supplied breakdown products to folate synthesis precursors: pABAGlu was hydrolysed to p-aminobenzoate and glutamate, and dihydropterin-6-aldehyde was reduced to 6-hydroxymethyldihydropterin. Fourth, both these reactions were detected in vitro; the reduction used NADPH as cofactor. An alternative salvage route for pABAGlu, direct reincorporation into dihydrofolate via the action of dihydropteroate synthase, appears implausible from the properties of this enzyme. We conclude that plants are excellent organisms in which to explore the biochemistry of folate salvage.
Orsomando
Giuseppe
G
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.
Bozzo
Gale G
GG
de la Garza
Rocío Díaz
RD
Basset
Gilles J
GJ
Quinlivan
Eoin P
EP
Naponelli
Valeria
V
Rébeillé
Fabrice
F
Ravanel
Stéphane
S
Gregory
Jesse F
JF
Hanson
Andrew D
AD
D017360:Arabidopsis / Q000378:metabolism; D004094:Dihydropteroate Synthase / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry / Q000378:metabolism*; D005971:Glutamates / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D018551:Solanum lycopersicum / Q000378:metabolism; D018532:Pisum sativum / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D011622:Pterins / Q000737:chemistry / Q000378:metabolism
D005971:Glutamates; D011622:Pterins; D005492:Folic Acid; C011398:4-aminobenzoylglutamic acid; D004094:Dihydropteroate Synthase