Recombinant tegumental protein Shistosoma japonicum very lowdensity lipoprotein binding protein as a vaccine candidate against Schistosoma japonicum.
A polyhistidine-tagged recombinant tegumental protein Schistosoma japonicum very lowdensity lipoprotein binding protein (SVLBP) from adult Schistosoma japonicum was expressed in Escherichia coli. The affinity purified rSVLBP was used to vaccinate mice. The worm numbers and egg deposition recovered from the livers and veins of the immunized mice were 33.5% and 47.6% less than that from control mice, respectively (p<0.05). There was also a marked increase in the antibody response in vaccinated mice: the titer of IgG1 and IgG2a, IgG2b in the vaccinated group was significantly higher than that in the controls (>1:6,400 in total IgG). In a comparison of the reactivity of sera from healthy individuals and patients with rSVLBP, recognition patterns against this parasite tegumental antigen varied among different groups of the individuals. Notably, the average titres of anti-rSVLBP antibody in sera from faecal egg-negative individuals was significantly higher than that in sera from the faecal egg-positives, which may be reflect SVLBP-specific protection. These results suggested that the parasite tegumental protein SVLBP was a promising candidate for further investigation as a vaccine antigen for use against Asian schistosomiasis.
Gan
Xiao-Xian
XX
Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
Shen
Li-Ying
LY
Wang
Yue
Y
Ding
Jian-Zu
JZ
Shen
Hui-Ying
HY
Zeng
Xiao-Peng
XP
McManus
Donald P
DP
Brindley
Paul J
PJ
Fan
Jinjiang
J
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006639:Histidine / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008079:Lipoproteins, VLDL / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010270:Parasite Egg Count; D011485:Protein Binding / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000517:prevention & control; D014614:Vaccines, Synthetic / Q000276:immunology
D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D008079:Lipoproteins, VLDL; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; C033223:polyhistidine; D006639:Histidine