The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane.
Fluorescent cell division cycle (CDC)48 proteins were studied in living plant protoplasts. CDC48A and somatic embryogenesis receptor like kinase 1 (SERK1) were found to co-localize in the endoplasmatic reticulum (ER) and at the plasma membrane (PM), but not in endosomal compartments. Fluorescent lifetime imaging microscopy (FLIM) was used to detect Förster resonance energy transfer (FRET) between CrFP/YFP-tagged CDC48A and SERK1. FRET is indicative of direct protein-protein interaction. CDC48A was found to interact only with SERK1 in small areas at the PM, but not in endosomes. These findings confirm and extend our previous findings that CDC48A in plants directly interacts with SERK1.
Aker
José
J
Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands.
Borst
Jan Willem
JW
Karlova
Rumyana
R
de Vries
Sacco
S
D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D008164:Luminescent Proteins / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism
D029681:Arabidopsis Proteins; D008164:Luminescent Proteins; D010770:Phosphotransferases