Cloning of the feline GADD45 cDNA and analysis of its mutation in feline lymphoma cell lines.
Growth arrest and DNA damage-inducible protein 45 (GADD45) plays an important role in suppressing multistep carcinogenesis. In this report, we describe the isolation of the complete wild-type feline GADD45 cDNA from feline tissues. Expression of feline GADD45 mRNA was detected in the liver, spleen, kidney, lung, and testis. The predicted amino acid sequences encoded by the full-length feline GADD45 cDNA display sequence homology with those from other vertebrates, and as in the case of human GADD45, cell growth suppression was observed by ectopic expression of feline GADD45. However, no mutations were detected by sequence analysis of feline GADD45 in several feline lymphoma cell lines, indicating that the GADD45 mutation might be uncommon in feline oncogenesis.
Oikawa
Tatsuo
T
Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan.
Okuda
Masaru
M
Kaneko
Naoki
N
Watanabe
Malaika
M
Hiraoka
Hiroko
H
Itamoto
Kazuhito
K
Nakaichi
Munekazu
M
Mizuno
Takuya
T
Inokuma
Hisashi
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000235:genetics*; D002415:Cats; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016153:Genes, Suppressor; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008223:Lymphoma / Q000235:genetics / Q000662:veterinary*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D014162:Transfection / Q000662:veterinary; D000097642:GADD45 Proteins
D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D000097642:GADD45 Proteins