title: Presence of unintended Agrobacterium tumefaciens cloning vector sequences in genetically modified plants.
abstract: Agrobacterium transformation was used in the production of genetically modified plants from oilseed rape (Brassica napus) and tobacco (Nicotiana tabacum). After inoculation stop with the antibiotic timentin, a subsequent one-week treatment eliminated the vector bacterium from the oilseed rape plate explant cultures. From the tobacco, however, we recorded vector-derived signals one week after potting the regenerants in the greenhouse and still 10 weeks later. Genetically modified plants produced through Agrobacterium-transformation therefore cannot be guaranteed to be completely free of unintended vector sequences after antibiotic treatment.
authors.affiliation: Finnish Environment Institute, Research Department, Hakuninmaantie 6, FI-00430, Helsinki, Finland. katarina.bjorklof@ymparisto.fi
authors.forename: Katarina
authors.initials: K
authors.lastname: Björklöf
authors.forename: Michael
authors.initials: M
authors.lastname: Färdig
authors.forename: Kirsten S
authors.initials: KS
authors.lastname: Jørgensen
mesh_terms: D016960:Agrobacterium tumefaciens / Q000502:physiology*; D001483:Base Sequence; D029688:Brassica napus / Q000235:genetics / Q000382:microbiology; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology*; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology; D014169:Transformation, Bacterial / Q000502:physiology*