Usefulness of intraovarian artery pulsatility and resistance indices measurement on the day of follicle aspiration for the assessment of oocyte quality.
OBJECTIVE
To examine the correlation between the pulsatility and resistance indices (PI and RI) of the intraovarian artery on the day of follicle aspiration and the oocyte quality, intrafollicular oxidative stress, and luteinization. Pulsatility index and RI on the day of hCG administration also were measured.


METHODS
Prospective study.


METHODS
Obstetrics and gynecology department of a university medical school in Japan.


METHODS
Thirty-five patients in an IVF-ET program.


METHODS
The PI and RI of the intraovarian artery were measured by transvaginal color ultrasonographic pulsed wave Doppler on the day of hCG administration and the day of follicle aspiration. Follicular fluids and the granulosa cells were collected at follicle aspiration.


METHODS
The PI and RI of the intraovarian artery blood flow on the day of hCG administration and of follicle aspiration, as well as the rate of development of mature oocytes, follicular fluid steroid levels, and the incidence of apoptosis in the granulosa cells.


RESULTS
The PI and RI on the day of follicle aspiration were correlated positively with the rate of mature oocytes retrieved (PI: r = 0.429; RI: r = 0.348), were correlated negatively with the incidence of apoptotic mural (PI: r = -0.383; RI: r = -0.459) and cumulus (PI: r = -0.378; RI: r = -0.469) granulosa cells, and were negatively correlated with the concentration of P in the follicular fluid (PI: r = -0.429; RI: r = -0.359). The PI and RI on the day of hCG administration were negatively correlated only with the total number of retrieved oocytes (PI: r = -0.393; RI: r = -0.374).


CONCLUSIONS
The PI and RI of the intraovarian artery blood flow measured on the day of follicle aspiration may be good indicators of the follicle luteinization and oxidation as well as of oocyte quality.
Du
Botao
B
Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata, Japan.
Takahashi
Kazuhiro
K
Ishida
Gabriela M
GM
Nakahara
Kenji
K
Saito
Hidekazu
H
Kurachi
Hirohisa
H
D000328:Adult; D017209:Apoptosis; D001158:Arteries / Q000000981:diagnostic imaging; D006063:Chorionic Gonadotropin / Q000627:therapeutic use; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry; D006107:Granulosa Cells; D006801:Humans; D007246:Infertility / Q000503:physiopathology*; D009865:Oocytes; D009994:Osmolar Concentration; D006080:Ovarian Follicle; D010053:Ovary / Q000098:blood supply*; D011374:Progesterone / Q000032:analysis; D011674:Pulse; D013396:Suction; D013997:Time Factors; D020858:Tissue and Organ Harvesting; D018617:Ultrasonography, Doppler, Pulsed; D014655:Vascular Resistance
D006063:Chorionic Gonadotropin; D011374:Progesterone