Characterisation of protein and antigen variability among Mycoplasma mycoides subsp. mycoides (LC) and Mycoplasma agalactiae field strains by SDS-PAGE and immunoblotting.
Mycoplasma mycoides subsp. mycoides (LC) (Mmm LC) and Mycoplasma agalactiae are the most important mycoplasma species involved in the contagious agalactia syndrome. A total of 25 field strains from Spain and the two type strains were analysed by SDS-PAGE and immunoblotting. Two polyclonal antisera (PAbs) raised against a pool of strains of each mycoplasma species were used. The results revealed a high degree of protein variability among the field strains. The type strain of Mmm LC appeared to be representative of the field strains of this species, whereas this was not the case with the M. agalactiae type strain. Whereas M. agalactiae is known to possess a gene family regulating surface antigen diversity, there is a need to study the mechanisms used byMmm LC to generate antigenic variability in more detail.
de la Fe
C
C
Unit of Epidemiology and Preventive Medicine, Veterinary Faculty, University of Las Palmas, Trasmontaña s/n. 35416 Arucas, Gran Canaria, Spain. cdelafe@becarios.ulpgc.es
Assunção
P
P
Rosales
R S
RS
Antunes
T
T
Poveda
J B
JB
D000818:Animals; D000940:Antigenic Variation; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary; D045644:Mycoplasma agalactiae / Q000145:classification / Q000235:genetics*; D009176:Mycoplasma mycoides / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011002:Pleuropneumonia, Contagious / Q000382:microbiology; D013045:Species Specificity