title: Acanthamoeba castellanii promotion of in vitro survival and transmission of coxsackie b3 viruses.
abstract: This work was undertaken to determine whether Acanthamoeba could play a role in the survival and transmission of coxsackieviruses and focused on in vitro interactions between Acanthamoeba castellanii and coxsackie B3 viruses (CVB-3). Residual virus titer evaluations and immunofluorescence experiments revealed a remarkable CVB-3 adsorption on amoeba surfaces and accumulation inside cells. The survival of viruses was independent of the dynamics of amoeba replication and encystment. In addition, our results indicated that virus-infected amoebas can release infectious viruses during interaction with human macrophages. On the basis of these data, Acanthamoeba appears to be a potential promoter of the survival of coxsackieviruses and their transmission to human hosts.
authors.affiliation: Dipartimento di Scienze del Farmaco, Via Muroni 23/A, 07100 Sassari, Italy. dsfanto@uniss.it
authors.forename: A
authors.initials: A
authors.lastname: Mattana
authors.forename: C
authors.initials: C
authors.lastname: Serra
authors.forename: E
authors.initials: E
authors.lastname: Mariotti
authors.forename: G
authors.initials: G
authors.lastname: Delogu
authors.forename: P L
authors.initials: PL
authors.lastname: Fiori
authors.forename: P
authors.initials: P
authors.lastname: Cappuccinelli
mesh_terms: D048248:Acanthamoeba castellanii / Q000254:growth & development / Q000502:physiology* / Q000821:virology*; D000818:Animals; D002462:Cell Membrane / Q000821:virology; D004199:Disease Vectors; D004770:Enterovirus / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008264:Macrophages / Q000648:ultrastructure / Q000821:virology; D050296:Microbial Viability; D011991:Receptors, Virus / Q000502:physiology*; D013997:Time Factors; D014779:Virus Replication
chemical_list: D011991:Receptors, Virus; C105941:coxsackievirus B receptor