title: Ghrelin stimulates phagocytosis and superoxide production in fish leukocytes.
abstract: To clarify the role of ghrelin in the fish immune system, the in vitro effect of ghrelin was examined in phagocytic leukocytes of rainbow trout (Oncorhynchus mykiss). Administration of trout ghrelin and des-VRQ-trout ghrelin, in which three amino acids are deleted from trout ghrelin, increased superoxide production in zymosan-stimulated phagocytic leukocytes from the head kidney. Gene expression of growth hormone (GH) secretagogue-receptor (GHS-R) was detected by RT-PCR in leukocytes. Pretreatment of phagocytic leukocytes with a GHS-R antagonist, [D-Lys3]-GHRP-6, abolished the stimulatory effects of trout ghrelin and des-VRQ-trout ghrelin on superoxide production. Ghrelin increased mRNA levels of superoxide dismutase and GH expressed in trout phagocytic leukocytes. Immunoneutralization of GH by addition of anti-salmon GH serum to the medium blocked the stimulatory effect of ghrelin on superoxide production. These results suggest that ghrelin stimulates phagocytosis in fish leukocytes through a GHS-R-dependent pathway, and also that the effect of ghrelin is mediated, at least in part, by GH secreted by leukocytes.
authors.affiliation: Freshwater Fisheries Research Department, National Research Institute of Fisheries Science, 2482-3 Chugushi, Nikko, Tochigi 321-1661, Japan. yadat@fra.affrc.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Yada
authors.forename: H
authors.initials: H
authors.lastname: Kaiya
authors.forename: K
authors.initials: K
authors.lastname: Mutoh
authors.forename: T
authors.initials: T
authors.lastname: Azuma
authors.forename: S
authors.initials: S
authors.lastname: Hyodo
authors.forename: K
authors.initials: K
authors.lastname: Kangawa
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000235:genetics; D054439:Ghrelin; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D036361:Peptide Hormones / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis; D043562:Receptors, G-Protein-Coupled / Q000032:analysis / Q000037:antagonists & inhibitors; D054440:Receptors, Ghrelin; D013482:Superoxide Dismutase / Q000032:analysis; D013481:Superoxides / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology
chemical_list: D054439:Ghrelin; D009842:Oligopeptides; D036361:Peptide Hormones; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D054440:Receptors, Ghrelin; D013481:Superoxides; C041048:growth hormone releasing hexapeptide; D013006:Growth Hormone; D015054:Zymosan; D013482:Superoxide Dismutase