[Establishment and application of fetal rolling palate organ culture model of mouse].
OBJECTIVE
To investigate the effect of all-trans retinoic acid (atRA) on the development of mouse palates and its possible mechanism, a model of fetal mouse rolling plate organ culture was establishted.


METHODS
Mouse embryonic palates were explanted on GD12 and cultured in a roller device for 72h induced by atRA in different concentrations from 10(-5) micromol/L to 10(-1) micromol/L.


RESULTS
Similar to development in vivo, palates in control normal fused. Compared with the control, palates development and fusion were promoted in group of 10(-15) micromol/L, while were inhibited in groups of 10(-4) micromol/L or greater, resulting in cleft palate. The proportion of fusion was reduced in a significant dose-response pattern.


CONCLUSIONS
In the model, the cultured palates continued developing and fusing. Palatal fusion was promoted by normally atRA in 10(-5) micromol/L level and were inhibited by atRA in 10(-4) micromol/L level or greater, which resulting in cleft palate. These mentioned confirm the validity and reliability of this model.
Han
Jing
J
Department of Food Science and Nutrition, School of Public Health, Peking University, Beijing 100083, China.
Xiao
Ying
Y
Lin
Jiu-Xiang
JX
Zhang
Zhao-Feng
ZF
Li
Yong
Y
D000818:Animals; D002972:Cleft Palate / Q000139:chemically induced*; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D023421:Models, Animal; D009924:Organ Culture Techniques / Q000379:methods*; D010159:Palate / Q000002:abnormalities / Q000196:embryology*; D011247:Pregnancy; D014212:Tretinoin / Q000633:toxicity*
D014212:Tretinoin