title: Effects of HeNe laser irradiation on experimental paracoccidioidomycotic lesions.
abstract: Paracoccidioidomycosis (PCM) is the most prevalent human mycosis in Latin America. The infection is thought to take place firstly in the lungs and then may disseminate to other organs and tissues. Treatment by currently available antifungals is lengthy, the drugs may have undesirable side effects, and some are costly. Occasional resistant strains of Paracoccidioides brasiliensis, the causative agent of PCM, have been reported. So, the search for more efficient treatments or adjuvant therapies has to be continued. In this work, we evaluated the effects of HeNe laser irradiation on cutaneous inflammatory lesions caused by the inoculation of 5 x 10(6)/0.1ml yeasts cells into the back footpad of Balb/c mice. HeNe irradiation (lambda=632.8nm, 3mW, incident energy of 3J/cm(2)) was applied at days 7, 8 and 9 post-infection and histological and immunohistochemical analysis were done. Unirradiated animals were used as controls. The results showed that laser-treated mice presented reduction of footpad edema, faster cutaneous wound healing, confluent granuloma, diffuse- and more loosely distributed immunolabeling for TNF-alpha, enhanced labeling of IFN-gamma and any P. brasiliensis form detected, whereas multiple viable fungi were seen in diffuse widespread granulomas obtained from non-treated mice foot-pad. Fungi that were harvested from laser-treated animals presented no capability of growth in vitro as compared to those obtained from non-treated mice. We conclude that HeNe laser irradiation was able to inhibit the progress of inflammatory local reaction produced by P. brasiliensis infection and influence local cytokines production. We suggest that this treatment modality can be a useful coadjuvant tool to be combined with antifungal agents in the treatment of PCM ulcerations. The mechanisms involved in laser therapy of PCM lesions need further investigation.
authors.affiliation: Department of Microbiology and Immunology, Institute of Biology, State University at Campinas (UNICAMP), Caixa Postal 6109, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Maria Carolina
authors.initials: MC
authors.lastname: Ferreira
authors.forename: Vânia Nieto
authors.initials: VN
authors.lastname: Brito
authors.forename: Jacy
authors.initials: J
authors.lastname: Gameiro
authors.forename: Maria Renata Salles Nogueira
authors.initials: MR
authors.lastname: Costa
authors.forename: Elza Costa Cruz
authors.initials: EC
authors.lastname: Vasconcellos
authors.forename: Maria Alice
authors.initials: MA
authors.lastname: Cruz-Hofling
authors.forename: Liana
authors.initials: L
authors.lastname: Verinaud
mesh_terms: D000818:Animals; D006371:Helium; D007150:Immunohistochemistry; D007834:Lasers; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009356:Neon; D010229:Paracoccidioidomycosis / Q000382:microbiology / Q000473:pathology / Q000628:therapy*
chemical_list: D006371:Helium; D009356:Neon