[Protective effect of selenium against T-2 toxin-induced inhibition of chondrocyte aggrecan and collagen II synthesis].
OBJECTIVE
To study the inhibitory effect of T-2 toxin on the expression of aggrecan and collagen II in chondrocytes and the protection of selenium against this effect.


METHODS
Human chondrocytes cultured in vitro were treated with T-2 toxin at different concentrations for varied time periods (1-5 days), and the cell viability was measured by MTT assay. Aggrecan expression was detected by toluidine blue staining and collagen II expression by immunostaining using monoclonal antibody of collagen. Aggrecan and collagen II mRNA expressions were measured by semiquantitative RT-PCR.


RESULTS
T-2 toxin dose- and time-dependently affected chondrocyte viability within the concentration range of 0.001-2 mg/L, the prolonged treatment time further enhanced the dose dependence of the inhibitory effect. T-2 toxin lowered aggrecan and collagen II synthesis in the chondrocytes and reduced their mRNA expressions. Selenium could partly attenuate the inhibitory effects of T-2 toxin on aggrecan mRNA expression, but showed no such effect against T-2-induced collagen II expression.


CONCLUSIONS
T-2 toxin can obviously inhibit aggrecan and collagen II synthesis in human chondrocytes, and selenium can partly antagonize the inhibitory effects of T-2 toxin on aggrecan.
Chen
Jing-hong
JH
Institute of Endemic Diseases, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
Cao
Jun-ling
JL
Chu
Yong-lie
YL
Yang
Zhan-tian
ZT
Shi
Zhong-li
ZL
Wang
Hong-lin
HL
Guo
Xiong
X
Wang
Zhi-lun
ZL
D053674:Aggrecans / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D024043:Collagen Type II / Q000096:biosynthesis* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005333:Fetus; D006801:Humans; D020011:Protective Agents / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012643:Selenium / Q000494:pharmacology*; D013605:T-2 Toxin / Q000633:toxicity*
D053674:Aggrecans; D024043:Collagen Type II; D020011:Protective Agents; D012333:RNA, Messenger; D012643:Selenium; D013605:T-2 Toxin