Mannose hyperbranched dendritic polymers interact with clustered organization of DC-SIGN and inhibit gp120 binding.
DC-SIGN (dendritic cell-specific ICAM-3 grabbing non-integrin) is a C-type lectin receptor of dendritic cells and is involved in the initial steps of numerous infectious diseases. Surface plasmon resonance has been used to study the affinity of a glycodendritic polymer with 32 mannoses, to DC-SIGN. This glycodendrimer binds to DC-SIGN surfaces in the submicromolar range. This binding depends on a clustered organization of DC-SIGN mimicking its natural organization as microdomain in the dendritic cells plasma membrane. Moreover, this compound inhibits DC-SIGN binding to the HIV glycoprotein gp120 with an IC50 in the micromolar range and therefore can be considered as a potential antiviral drug.
Tabarani
Georges
G
Institut de Biologie Structurale, UMR 5075 CEA/CNRS/Université Joseph Fourier, 41, rue Jules Horowitz, 38027 Grenoble Cedex 1, France.
Reina
José J
JJ
Ebel
Christine
C
Vivès
Corinne
C
Lortat-Jacob
Hugues
H
Rojo
Javier
J
Fieschi
Franck
F
D001704:Biopolymers / Q000737:chemistry* / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000187:drug effects* / Q000378:metabolism; D037181:Lectins, C-Type / Q000737:chemistry* / Q000378:metabolism; D008358:Mannose / Q000737:chemistry*; D015394:Molecular Structure; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D001704:Biopolymers; D015815:Cell Adhesion Molecules; D015699:HIV Envelope Protein gp120; D037181:Lectins, C-Type; D008358:Mannose; D011956:Receptors, Cell Surface; D012996:Solutions; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin