Validation of osmotic fragility test and dichlorophenol indophenol precipitation test for screening of thalassemia and Hb E.
The strategy for screening of thalassemia and Hb E by a combination of osmotic fragility (OF) test and dichlorophenol indophenol precipitation (DCIP) test was validated with 436 unrelated Thai subjects. Hemoglobin (Hb) typing, Hb A2 quantitation, PCR and DNA sequence analysis were used as confirmatory methods for diagnosis of thalassemia and hemoglobinopathy. The sensitivity and specificity of this strategy was 100% and 79.7%, respectively. The results assessed by two medical scientists were exactly the same with 93.3% accuracy in comparison with the confirmatory methods. A combination of OF and DCIP has been shown to be a reliable, rapid, simple and sensitive strategy for screening thalassemia and Hb E in the Thai population.
Sangkitporn
Siripakorn
S
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Sangkitporn
Somchai
S
Sangnoi
Areerat
A
Supangwiput
Ornchira
O
Tanphaichitr
Voravarn S
VS
D015086:2,6-Dichloroindophenol; D005820:Genetic Testing / Q000379:methods*; D006403:Hematologic Tests; D006446:Hemoglobin E / Q000032:analysis*; D006453:Hemoglobinopathies / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D017422:Sequence Analysis, DNA; D013789:Thalassemia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics
D006446:Hemoglobin E; D015086:2,6-Dichloroindophenol