title: Estimating equivalency values of microbial phytase for amino acids in growing and finishing pigs fitted with steered ileo-cecal valve cannulas.
abstract: Ten crossbred barrows (48.3 +/- 2.3 kg of initial BW) fitted with steered ileo-cecal valve cannulas were used to investigate the effects of supplemental microbial phytase on the apparent ileal digestibilities (AID) of AA, Ca, P, N, and DM, and the apparent total tract digestibilities of Ca, P, N, and DM. All diets were corn-soybean meal-based, and contained 0.44% Ca and 0.40% total P. Diets 1, 2, and 3 contained 12.0, 11.1, and 10.2% CP, respectively. Diets 4 and 5 had the same ingredient composition as diet 3, plus 250 and 500 U/kg phytase (Natuphos), respectively. Pigs were randomly allotted to 1 of 5 dietary treatments in a paired 5 x 5 Latin square with an extra period to test for carryover effects. Each 14-d period consisted of a 7-d adjustment followed by a 3-d total collection, a 12-h ileal digesta collection, a 3-d readjustment, and a second 12-h ileal digesta collection. Pigs were housed individually in metabolism pens (1.2 x 1.2 m). Water was supplied ad libitum, and feed was supplied at a level of 9% of the metabolic BW (BW(0.75)) per day in 2 equal daily feedings. As the dietary CP concentration increased, the AID of CP and all AA measured increased linearly (P < 0.05) with the exception of proline. In addition, the apparent total tract digestibilities (grams per day) and retention of N (grams per day) increased linearly (P < 0.01) with increasing CP levels. Supplementing diets with phytase increased the AID of Ca (P < 0.01), P (P < 0.001), CP (P = 0.07), and the AA (P < 0.10) Gly, Ala, Val, Ile, Thr, TSAA, Asp, Glu, Phe, Lys, and Arg. Protein and phytase response equations were generated for those AA affected (P < 0.10) by both CP level and phytase supplementation. Based on these equations, 500 U/kg of phytase can replace 0.52 percentage units of the dietary CP, which includes a 0.03 percentage unit improvement in Lys AID. The results of this study show that supplementing pig diets with microbial phytase improves CP and AA digestibilities in addition to Ca and P digestibilities.
authors.affiliation: Department of Animal Sciences, Purdue University, West Lafayette, IN, USA. jradclif@purdue.edu
authors.forename: J S
authors.initials: JS
authors.lastname: Radcliffe
authors.forename: R S
authors.initials: RS
authors.lastname: Pleasant
authors.forename: E T
authors.initials: ET
authors.lastname: Kornegay
mesh_terms: D010832:6-Phytase / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004032:Diet; D004063:Digestion; D007082:Ileum / Q000502:physiology; D008297:Male; D010758:Phosphorus / Q000378:metabolism; D013552:Swine / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D010758:Phosphorus; D010832:6-Phytase; D002118:Calcium