Depletion of abundant proteins from non-human primate serum for biomarker studies.
Non-human primates are an important biomedical research model organism and offer great promise for serum biomarker proteomic studies. However, potential obstacles to these studies include affinity serum depletion methods based on human antigens, depletion methods altering quantitation, and incomplete non-human primate genome sequences for protein identification. In the present study, high-abundance protein removal from monkey serum using a human multiple affinity removal system (MARS) was shown to be specific and did not alter quantitation. Depleted serum also demonstrated greater sensitivity for previously masked, lower-abundance proteins.
Freeman
Willard M
WM
Department of Pharmacology H078, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA. wfreeman@psu.edu
Lull
Melinda E
ME
Guilford
Michael T
MT
Vrana
Kent E
KE
D000818:Animals; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008252:Macaca fascicularis; D015203:Reproducibility of Results; D044967:Serum
D015415:Biomarkers; D001798:Blood Proteins