Molecular typing of Pasteurella pneumotropica isolated from rodents by amplified 16S ribosomal DNA restriction analysis and pulsed-field gel electrophoresis.
A total of 52 isolates of Pasteurella pneumotropica obtained from rodents were examined for their genetic heterogeneity. On the basis of DNA restriction analysis, including amplified 16S ribosomal DNA restriction analysis (ARDRA) and pulsed-field gel electrophoresis (PFGE), differences were identified among the isolates. ARDRA typing with Hae III revealed 4 different banding patterns of the P. pneumotropica isolates. Eighty-two percent of the 23 isolates identified as a-1 were derived from mice, whereas all the isolates identified as a-3 were derived from rats. Most of the isolates, which showed hemolytic activity on blood agar, obtained from mice and rats, were identified as a-2 and a-4, respectively. By restriction analysis of genomic DNA, Apa I and Not I digestion differentiated 9 variants and an undiscriminating group. However, no close relation with regard to the phenotypic characteristics was observed among the variants. The isolates identified as a-2 and a-4 could not be distinguished by PFGE analysis. DNA restriction analysis revealed that the genetic diversity of the P. pneumotropica isolates was more complex than the phenotypic characteristics among the species, and that at least the P. pneumotropica isolates were clearly differentiated into 4 groups by ARDRA typing with Hae III.
Sasaki
Hiraku
H
Animal Research Center, Tokyo Medical University, Shinjuku-ku, Tokyo, Japan. h-sasaki@tokyo-med.ac.jp
Kawamoto
Eiichi
E
Okiyama
Emi
E
Ueshiba
Hidehiro
H
Mikazuki
Katsumi
K
Amao
Hiromi
H
Sawada
Takuo
T
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D014644:Genetic Variation; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D044142:Pasteurella pneumotropica / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D015183:Restriction Mapping / Q000379:methods
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases; C038485:GGGCCC-specific type II deoxyribonucleases