The C-terminal domain of the transcriptional corepressor CtBP is intrinsically unstructured.
C-terminal binding proteins (CtBPs) are moonlighting proteins involved in nuclear transcriptional corepression and in Golgi membrane tubule fission. Structural information on CtBPs is available for their substrate-binding domain, responsible for transcriptional repressor recognition/binding, and for the nucleotide-binding domain, involved in NAD(H)-binding and dimerization. On the contrary, little is known about the structure of CtBP C-terminal region ( approximately 90 residues), hosting sites for post-translational modifications. In the present communication we apply a combined approach based on bioinformatics, nuclear magnetic resonance, circular dichroism spectroscopy, and small-angle X-ray scattering, and we show that the CtBP C-terminal region is intrinsically unstructured in the full-length CtBP and in constructs lacking the substrate- and/or the nucleotide-binding domains. The flexible nature of this protein region, and its structural transitions, may be instrumental for CtBP recognition and binding to diverse molecular partners.
Nardini
Marco
M
Department of Biomolecular Sciences and Biotechnology, and CNR-INFM, University of Milano, I-20131 Milano, Italy.
Svergun
Dmitri
D
Konarev
Peter V
PV
Spanò
Stefania
S
Fasano
Mauro
M
Bracco
Chiara
C
Pesce
Alessandra
A
Donadini
Alessandra
A
Cericola
Claudia
C
Secundo
Francesco
F
Luini
Alberto
A
Corda
Daniela
D
Bolognesi
Martino
M
D000429:Alcohol Oxidoreductases; D000595:Amino Acid Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D004268:DNA-Binding Proteins; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010750:Phosphoproteins; D017510:Protein Folding; D012097:Repressor Proteins / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D012097:Repressor Proteins; D014157:Transcription Factors; D000429:Alcohol Oxidoreductases; C096794:C-terminal binding protein