[Morphological changes of ECV304 cells infected by herpes simplex virus type 2].
OBJECTIVE
To observe the pathological changes and morphological alterations of ECV304 cells after the infection by herpes simplex virus type 2 (HSV-2) in vitro.


METHODS
Passaged ECV304 cells were infected with HSV-2, TCID50 and morphological changes were observed by optical microscopy and tissue staining.


RESULTS
One day after HSV-2 infection, swelling, rounding, and increase of thickened cytoplasmic granules occurred in the ECV304 cells, and on day 2, cell fusion was observed with weakened nuclear staining.


CONCLUSIONS
ECV304 cells mostly undergo necrosis after HSV-2 infection without obvious evidence of cell apoptosis.
Zhao
Hai-quan
HQ
Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China.
Ma
Wen-li
WL
Zhang
Ya-li
YL
Mo
Xiao-yang
XY
Ke
Chang-wen
CW
Zheng
Huan-ying
HY
Zheng
Wen-ling
WL
D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000473:pathology* / Q000821:virology*; D006566:Herpesviridae Infections / Q000473:pathology*; D018258:Herpesvirus 2, Human; D006801:Humans; D009336:Necrosis; D014471:Umbilical Veins / Q000473:pathology