title: Marsupial development in the mysid Neomysis integer (Crustacea: Mysidacea) to evaluate the effects of endocrine-disrupting chemicals.
abstract: Embryonic development is a crucial time window within an organism's life history. Relatively few studies have focused on understanding the potential effects of endocrine disruptors on embryogenesis in invertebrates. Mysids (Crustacea: Mysidacea) have been used extensively in regulatory toxicity testing and they are the only invertebrate model currently included in the U.S. EPA's Endocrine Disruptor Screening and Testing Program. We developed a method for studying mysid embryonic development in multiwell plates until the release of free-swimming juveniles. This method was used to evaluate the potential effects of the insecticide methoprene, a juvenile hormone analog, on mysid embryogenesis. Embryos were exposed to nominal concentrations 0.01, 1, and 100 microg methoprene/L. Average percentage survival, hatching success, total development time and duration of each developmental stage were analyzed. Embryos exposed to 1 and 100 microg methoprene/L had a significantly lower hatching success and lower survival rates. Our study indicates that in vitro embryogenesis can be used as a valuable tool to study the impact of endocrine disruptors in mysids.
authors.affiliation: Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, J. Plateaustraat 22, B-9000 Ghent, Belgium. an.ghekiere@ugent.be
authors.forename: A
authors.initials: A
authors.lastname: Ghekiere
authors.forename: N
authors.initials: N
authors.lastname: Fockedey
authors.forename: T
authors.initials: T
authors.lastname: Verslycke
authors.forename: M
authors.initials: M
authors.lastname: Vincx
authors.forename: C R
authors.initials: CR
authors.lastname: Janssen
mesh_terms: D000818:Animals; D003445:Crustacea / Q000187:drug effects* / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D008726:Methoprene / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D007306:Insecticides; D008726:Methoprene