title: [Preparation and purification of polyclonal antibody against chymopapain].
abstract: OBJECTIVE
  To prepare and purify polyclonal antibody against chymopapain, and to make a foundation for establishing an immunossay for chymopapain.
  
  
  METHODS
  New Zealand rabbit was immunized with chymopapain. Antiserum was purified by Protein A and analyzed by ELISA.
  
  
  RESULTS
  The titer of the antiserum obtained in this experiment by ELISA was up to 1:380000 and the purity was proved to be high by SDS-PAGE.
authors.affiliation: Guangzhou General Hospital of Guangzhou Military Area, Guangzhou 510010, China.
authors.forename: Yu-lan
authors.initials: YL
authors.lastname: Ding
authors.forename: Tai-cheng
authors.initials: TC
authors.lastname: Yang
authors.forename: Shu-jin
authors.initials: SJ
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D031042:Asimina / Q000737:chemistry*; D002917:Chymopapain / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D010206:Papain / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits
chemical_list: D000906:Antibodies; D010206:Papain; D002917:Chymopapain