title: Top-down proteomic analysis of the soluble sub-proteome of the obligate thermophile, Geobacillus thermoleovorans T80: insights into its cellular processes.
abstract: We report the first analysis of the soluble sub-proteome of the obligate thermophile, Geobacillus thermoleovorans T80, utilizing a robust multidimensional protein identification protocol. A total of 1,336 proteins were initially identified utilizing automated MS/MS identification software. Intensive manual curation resulted in a final list containing a total of 294 unique proteins. Physiochemical characterization and functional classification of the soluble sub-proteome was carried out. The strategy has allowed us to gain an insight into the cellular processes of this obligate thermophile, identifying a variety of proteins known to play a role in stress response. Included within these were a number of sigma factors such as sigma(A) that initiate transcription of the heat shock operons controlled by the HrcA-CIRCE complex within gram positive bacteria. In addition, it has enabled us to assign a degree of functionality to 29 out of 36 gene products detected in this study that were hitherto described as being only hypothetical conserved proteins.
authors.affiliation: School of Biomedical Sciences, University of Ulster, Coleraine, County Londonderry, BT52 1SA, United Kingdom. rl.graham@ulster.ac.uk
authors.forename: Robert Leslie James
authors.initials: RL
authors.lastname: Graham
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Pollock
authors.forename: Nigel G
authors.initials: NG
authors.lastname: Ternan
authors.forename: Geoff
authors.initials: G
authors.lastname: McMullan
mesh_terms: D001406:Bacillaceae / Q000737:chemistry* / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry* / Q000502:physiology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D030562:Databases, Protein; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012995:Solubility; D014357:Trypsin / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D014357:Trypsin