title: Otomicroscopic and histologic findings of induced myringosclerosis in rats: a critical study of an experimental model.
abstract: Myringosclerosis is characterized by hyaline changes of the lamina propria of the tympanic membrane. Experimental studies have used otomicroscopy or histology to evaluate myringosclerosis in animals, but they do not correlate precisely these two methods.
  
  
  OBJECTIVE
  The present study evaluates the accuracy of otomicroscopy in the diagnosis of myringosclerosis in rats.
  
  
  METHODS
  Experimental.
  
  
  METHODS
  Myringosclerosis was induced by transtympanic inoculation of Streptococcus pneumoniae in 25 Wistar rats, which were examined weekly through otomicroscopy and sacrificed after eight weeks for histologic study of their tympanic membranes.
  
  
  RESULTS
  From the comparison of the otomicroscopic data in relation to the histologic findings, we could observe sensibility of 80% and specificity of 75% for the otomicroscopy.
  
  
  CONCLUSIONS
  Considering the results in this study, otomicroscopy did not represent a good method to evaluate myringosclerosis in this experimental model.
authors.affiliation: Center of Health Sciences, Federal University of Pernambuco.
authors.forename: Patrícia F
authors.initials: PF
authors.lastname: Santos
authors.forename: Mariana C
authors.initials: MC
authors.lastname: Leal
authors.forename: Cristina
authors.initials: C
authors.lastname: Peixoto
authors.forename: Silvio
authors.initials: S
authors.lastname: Caldas Neto
authors.forename: Silvania Tavares Paz
authors.initials: ST
authors.lastname: Rosas
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D010033:Otitis Media / Q000473:pathology*; D038781:Otoscopy / Q000379:methods; D011008:Pneumococcal Infections / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D012598:Sclerosis; D013296:Streptococcus pneumoniae; D014432:Tympanic Membrane / Q000473:pathology* / Q000648:ultrastructure