title: [Purification of a new phospholipase A2 homologue from Agkistrodon blomhoffii siniticus and its effects on gene expression of Hep3B cells].
abstract: A new PLA2 homologue was purified from Agkistrodon blomhoffii Siniticus by applying reverse phase (HPLC) C18 column. The molecular weight of PLA2 homologue is 13900Da and its purity is 97.2%. The results of N-terminal sequence analysis were HLLQFRKMIKKMTKK. All the fragments were determined with the protein-protein BLAST software of GenBank. BLAST analysis showed that the N-terminal sequences of 15 amino acids were highly homologous with the sequences of other PLA2.
authors.affiliation: Instrumental Analysis and Research Center Southern Medical University, Guangzhou 510515, China.
authors.forename: An-de
authors.initials: AD
authors.lastname: Ma
authors.forename: Shao-yu
authors.initials: SY
authors.lastname: Wu
authors.forename: Jia-jie
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Zhi-qin
authors.initials: ZQ
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Le
authors.initials: L
authors.lastname: Yu
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Wen
authors.forename: Shu-guang
authors.initials: SG
authors.lastname: Wu
mesh_terms: D017836:Agkistrodon; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010741:Phospholipases A / Q000737:chemistry / Q000302:isolation & purification*; D054467:Phospholipases A2; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D012910:Snake Venoms / Q000737:chemistry*
chemical_list: D012910:Snake Venoms; D010741:Phospholipases A; D054467:Phospholipases A2