title: Determination of geniposide in rat urine after oral administration of the traditional Chinese medicinal preparation yin-zhi-ku decoction by high-performance liquid chromatography.
abstract: A high-performance liquid chromatography method with solid-phase extraction is introduced for the determination of geniposide in rat urine after oral administration of yin-zhi-ku decoction. Geniposide and an internal standard (paeoniflorin) are extracted from urine using Strata cartridges. Analysis of the extract is then performed on a reversed-phase C18 column using acetonitrile-water (14:86, v/v) as eluting solvent system. UV detection is set at 238 nm. The calibration curve for geniposide is linear (r = 0.9996) in the concentration range of 2.0-240 microg/mL. Both intra- and interday precision of the geniposide are determined, and their relative standard deviation does not exceed 10%. The validated method is successfully applied to determine geniposide from rat urine after oral administration of yin-zhi-ku decoction.
authors.affiliation: Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, PR China. yg4847@yahoo.com.cn
authors.forename: Guan
authors.initials: G
authors.lastname: Ye
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Hao-Long
authors.initials: HL
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Cheng-Gang
authors.initials: CG
authors.lastname: Huang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D039823:Iridoids / Q000652:urine*; D008297:Male; D011714:Pyrans / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D039823:Iridoids; D011714:Pyrans; C513823:Yin-Zhi-Ku; C007835:geniposide