[Evaluation of soluble platelet cell adhesion molecule sPECAM-1 and chemokine MCP-1 (CCL2) concentration in CSF of patients with tick-borne encephalitis].
Platelet-endothelial cell adhesion molecule-1 (PECAM-1) is a glycoprotein involved in the transendothelial migration of leukocytes and MCP-1 is a proinflammatory protein, member of the 1 subfamily of chemokines, acting as activator of specific leukocytes. The expression of PECAM-1 and MCP-1 was studied in various pathological processes, but very little is known about the significance of these molecules during inflammatory reaction in viral diseases of the nervous system, including tick-borne encephalitis.


METHODS
Cerebrospinal fluid was obtained from 17 patients with TBE, treated at the Department of Infection Diseases in Białystok. The level of sPECAM-1 and MCP-1 was evaluated by the ELISA method. The control group consisted of 18 patients in which the battery of tests, including CSF study, has excluded the organic character of the neurological syndrome. The U- Mann-Whitney test was applied to establish the statistical significance of the results.


RESULTS
The concentration of sPECAM-l and MCP-1 in cerebrospinal fluid of TBE patients was significantly increased in comparison with the. control group.


CONCLUSIONS
Elevated concentration of sPECAM-1 and MCP-1 may be a result of increased release from endothelical cells, activated T-cells, platelets and monocytes. Increased expression of sPECAM-1 is the significant but unspecific event in immunological processes in inflammatory reaction in TBE. The increased concentration of MCP-1 seems to be an essential event in the pathomechanism of inflammation in the central nervous system in TBE. It would be valuable in clinical conditions to search for the methods of inhibition of the increased expression of sPECAM-1 and MCP-1 in TBE to diminish the inflammatory reaction.
Michałowska-Wender
Grazyna
G
Polska Akademia Nauk w Poznaniu, Zespół Neuroimmunologii Instytutu Medycyny Doświadczalnej i Klinicznej. mwender@am.poznan.pl
Losy
Jacek
J
Kondrusik
Maciej
M
Zajkowska
Joanna
J
Pancewicz
Slawomir
S
Grygorczuk
Sambor
S
Wender
Mieczysław
M
D000328:Adult; D000368:Aged; D018932:Chemokine CCL2 / Q000134:cerebrospinal fluid*; D004675:Encephalitis, Tick-Borne / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000134:cerebrospinal fluid*
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D019408:Platelet Endothelial Cell Adhesion Molecule-1