Determination of conductivity of bacteria by using cross-flow filtration.
An important property of the bacterial surface is its conductivity. To obtain reliable conductivity values, it is essential to handle the cells as gently as possible during the measurement procedure. We have developed a method where a standard conductivity meter is used in combination with cross-flow filtration, thus avoiding repeated centrifugation and resuspension. With this method, the conductivity of Bacillus subtilis was determined to be 7,000 microS/cm, which is a deviation from previously published data by almost an order of a magnitude.
Johansson
LarsErik
L
Department of Biology and Chemical Engineering, Mälardalen University, P.O. Box 325, SE-631 05 Eskilstuna, Sweden. lars-erik.johansson@mdh.se
Aldaeus
Fredrik
F
Jonsson
Gunnar
G
Hamp
Sven
S
Roeraade
Johan
J
D001412:Bacillus subtilis / Q000166:cytology / Q000502:physiology*; D018407:Bacterial Physiological Phenomena; D004553:Electric Conductivity; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D009997:Osmotic Pressure; D015203:Reproducibility of Results