[The transformation of betA gene into the pollen plantlets of Populus simonii x P. nigra].
In this study, betA gene was introduced into the pollen plantlets of Populus simonii x P. nigra using Agrobacterium-mediated transformation. The four kanamycine-resistant plants obtained were identified as transgenic plants by PCR detection and the results were all positive. The result of quantitative real-time PCR detection showed that the betA gene was transcribed and expressed in all the transformed plants, but the transcript levels are different. Test of salt-tolerance of the transgenic plants showed that 80%-00% of transgenic plants were rooted while 0 of non-transgenic plants were rooted at 0.55% NaCl stress, and 0 of transgenic plants were rooted at 0.70%-0.80% NaCl stress. The betaine content analysis showed the betaine content of the transgenic plants are obviously higher than that in non-transgenic plants, so transformation betA gene raised the salt tolerance to the transgenic plants.
Liu
Gui-Feng
GF
Heilongjiang Provincial Key Laboratory of Teaching and Research Section of Forestry Genetics and Breeding, Northeast Forestry University, Harbin 150040, China. liuguifeng@126.com
Cheng
Gui-Lan
GL
Jiang
Jing
J
Bai
Shuang
S
Yu
Ying
Y
Cai
Zhi-Jun
ZJ
Dong
Jing-Xiang
JX
Li
Shu-Juan
SJ
D001622:Betaine / Q000378:metabolism; D050545:Choline Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D011058:Pollen / Q000166:cytology / Q000235:genetics*; D032107:Populus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology; D014170:Transformation, Genetic
D001622:Betaine; D012965:Sodium Chloride; D050545:Choline Dehydrogenase