Embryonic stem cells as a novel cell source of cell-based biosensors.
To investigate the use of embryonic stem cells as biosensor elements, mouse embryoid bodies were cultured on the surface of the light-addressable potentiometric sensor and induce to in vitro differentiate into cardiomyocytes and neurons. Extracellular potentials of the cells were recorded by sensor, to detect stem cells potential applications in drugs screening. The experimental results show that known cardiac stimulants (isoproterenol) and relaxants (carbamylcholine) have characteristic effects on the cardiomyocytes in terms of the changes of beat frequency, amplitude and duration. Thus, the embryonic stem cells potentially represent a renewable cell source for the cell-based biosensors.
Liu
Qingjun
Q
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
Huang
Huarong
H
Cai
Hua
H
Xu
Ying
Y
Li
Yan
Y
Li
Rong
R
Wang
Ping
P
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002217:Carbachol / Q000008:administration & dosage; D002316:Cardiotonic Agents / Q000008:administration & dosage*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007545:Isoproterenol / Q000008:administration & dosage; D051379:Mice; D008839:Microelectrodes; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D002316:Cardiotonic Agents; D002217:Carbachol; D007545:Isoproterenol