title: Effect of bioaugmentation of activated sludge with white-rot fungi on olive mill wastewater detoxification.
abstract: OBJECTIVE
  To test the potential use of Phanerochaete chrysosporium and other white-rot fungi to detoxify olive mill wastewaters (OMW) in the presence of a complex activated sludge. To combine the aerobic with anaerobic treatment to optimize the conversion of OMW in biogas.
  
  
  RESULTS
  A 25-l air lift reactor was used to pretreat OMW by white-rot fungi. Detoxification of the OMW was monitored by size exclusion HPLC analysis, chemical oxygen demand (COD)/biological oxygen demand (BOD(5)) ratio evolution, and bioluminescence toxicity test. Anaerobic treatment of OMW was performed in a 12-l anaerobic filter reactor. Efficiency of the treatment was evaluated by organic matter removal, and biogas production. By comparison with the pretreatment by activated sludge only, the bioaugmentation with Phanerochaete chrysosporium or Trametes versicolor led to high removal of organic matter, decreased the COD/BOD(5) ratio and the toxicity. The subsequent anaerobic digestion of the OMW pretreated with activated sludge-white-rot fungi showed higher biomethanization yields than that pretreated with activated sludge only. Higher loading rates (7 g COD l(-1) day(-1)) were reached without any acidification or inhibition of biomethanization.
  
  
  CONCLUSIONS
  The use of white-rot fungi, even in the presence of complex biological consortia to detoxify OMW, proved to be possible and made the anaerobic digestion of OMW for methane production feasible.
  
  
  CONCLUSIONS
  The use of fungi for OMW reuse and energy production could be adapted to industrial applications.
authors.affiliation: Centre de Biotechnologie de Sfax, BP: 'K', 3038 Sfax, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Dhouib
authors.forename: M
authors.initials: M
authors.lastname: Ellouz
authors.forename: F
authors.initials: F
authors.lastname: Aloui
authors.forename: S
authors.initials: S
authors.lastname: Sayadi
mesh_terms: D001673:Biodegradation, Environmental; D005227:Fatty Acids / Q000032:analysis; D007220:Industrial Waste; D000069463:Olive Oil; D020075:Phanerochaete / Q000378:metabolism*; D010938:Plant Oils; D012722:Sewage; D018508:Water Purification / Q000379:methods*
chemical_list: D005227:Fatty Acids; D007220:Industrial Waste; D000069463:Olive Oil; D010938:Plant Oils; D012722:Sewage