Selective cytotoxicity of recombinant STXA1-GM-CSF protein in hematopoetic cancer cells.
Chimeric proteins are composed of a cell-targeting moiety and a cell-killing moiety. In this study, a chimeric protein, STXA1-GM-CSF, composed of catalytic domain of Shiga toxin (A1) and granulocyte-macrophage colony-stimulating factor (GM-CSF) was constructed and expressed in E. coli. Cytotoxicity, receptor blocking, and neutralization experiments revealed that the chimeric protein induced cytotoxic effect on different cell lines. This effect was found to be specific, due to the presence of the killing moiety (A1), which exerts its effect through a specific GM-CSF-targeting domain, by binding to its receptor present on those cell lines. These initial investigations indicate that the chimeric protein was functional; further analyses are required for its application.
Roudkenar
M Habibi
MH
Molecular Biology Unit, Pasteur Institute of Iran, Tehran, Iran.
Jafari
A
A
Oloomi
M
M
Bouzari
S
S
D017209:Apoptosis; D002470:Cell Survival; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000633:toxicity*; D018922:HL-60 Cells; D019337:Hematologic Neoplasms / Q000503:physiopathology*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000503:physiopathology*; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor