Use of enzyme-linked immunosorbent assay for detection of natural leishmania infections in phlebotomine sand flies from southeastern Turkey.
In total, 320 phelebotomine sand flies from Alibozlu (Osmaniye), Kizyusuflu (Osmaniye), and Sanliurfa in southeastern Turkey were tested for the detection and identification of Leishmania in vector sand flies by enzyme-linked immunosorbent assay with species-specific monoclonal antibodies. We used monoclonal antibodies that recognize both Leishmania tropica and Leishmania major, and a monoclonal antibody specific only to L. tropica. Phosphate-buffered saline and monoclonal antibody M2 recognizing Leishmania amazonensis were used as controls. Infection rates of sand flies were 0.9% in Alibozlu, 0% Kizyusuflu, and 3.6% in Sanliurfa. Positive sand flies were identified as Phlebotomus sergenti Parrot and Phlebotomus major syriacus Adler & Theodor.
Pazarbasi
Ayfer
A
Department of Medical Biology and Genetics, School of Medicine, University of Cukurova, 01330 Balcali, Adana, Turkey.
Alptekin
Davut
D
Luleyap
H Umit
HU
Kasap
Mulkiye
M
Kasap
Halil
H
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D007303:Insect Vectors / Q000145:classification / Q000469:parasitology*; D007891:Leishmania / Q000276:immunology* / Q000302:isolation & purification*; D018320:Leishmania major / Q000302:isolation & purification; D007895:Leishmania tropica / Q000302:isolation & purification; D010691:Phlebotomus / Q000145:classification / Q000469:parasitology*; D015995:Prevalence; D013045:Species Specificity; D014421:Turkey
D000913:Antibodies, Protozoan