Electrogenerated chemiluminescence detection of amino acids based on precolumn derivatization coupled with capillary electrophoresis separation.
A novel method for highly sensitive detection of primary and secondary amino acids with selective derivatization using acetaldehyde as a new derivatization reagent was proposed by capillary electrophoresis (CE) coupled with electrogenerated chemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(II). The precolumn derivatization of these amino acids with acetaldehyde was performed in aqueous solution at room temperature for 1 h. Upon optimized derivatization, the ECL intensities and detection sensitivities of the amino acids were significantly enhanced by 20-70 times. Using four amino acids, arginine, proline, valine, and leucine, as model compounds, their derivatives could be completely separated by CE and sensitively detected by ECL within 22 min. The linear ranges were 0.5-100 microM for arginine and proline and 5-1000 microM for valine and leucine with the detection limits of 1 x 10(-7) (0.5 fmol, arginine), 8 x 10(-8) (0.4 fmol, proline), 1 x 10(-6) (5 fmol, valine), and 1.6 x 10(-6) M (8 fmol, leucine) at a signal-to-noise ratio of 3. The derivatization reactions and ECL process of amino acids were also proposed based on in situ Fourier transform infrared and ultraviolet spectrometric analyses.
Li
Jianguo
J
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China.
Yan
Qinyi
Q
Gao
Yunlong
Y
Ju
Huangxian
H
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D000079:Acetaldehyde / Q000737:chemistry*; D000596:Amino Acids / Q000032:analysis*; D056831:Coordination Complexes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D000079:Acetaldehyde; D000596:Amino Acids; D056831:Coordination Complexes; D012996:Solutions; D014867:Water; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds