title: Role of the alpha/beta interferon response in the acquisition of susceptibility to poliovirus by kidney cells in culture.
abstract: Replication of poliovirus (PV) is restricted to a few sites, including the brain and spinal cord. However, this neurotropism is not conserved in cultured cells. Monkey kidney cells become susceptible to PV infection after cultivation in vitro, and cell lines of monolayer cultures from almost any tissue of primates are susceptible to PV infection. These observations suggest that cellular changes during cultivation are required for acquisition of susceptibility. The molecular basis for the cellular changes during this process is not known. We investigated the relationship between PV susceptibility and interferon (IFN) response in primary cultured kidney and liver cells derived from transgenic mice expressing human PV receptor and in several primate cell lines. Both kidneys and liver in vivo showed rapid IFN response within 6 h postinfection. However, monkey and mouse kidney cells in culture and primate cell lines, which were susceptible to PV, did not show such rapid response or showed no response at all. On the other hand, primary cultured liver cells, which were partially resistant to infection, showed rapid IFN induction. The loss of IFN inducibility in kidney cells was associated with a decrease in expression of IFN-stimulated genes involved in IFN response. Mouse kidney cells pretreated with a small dose of IFN, in turn, restored IFN inducibility and resistance to PV. These results strongly suggest that the cells in culture acquire PV susceptibility during the process of cultivation by losing rapid IFN response that has been normally maintained in extraneural tissues in vivo.
authors.affiliation: Department of Microbiology and Immunology, Tokyo Metropolitan Institute for Neuroscience, Tokyo Metropolitan Organization for Medical Research, Musashidai, Fuchu-shi, Tokyo, 183-8526, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Takuya
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Miki
authors.initials: M
authors.lastname: Ida-Hosonuma
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Yoneyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujita
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Horie
authors.forename: Miwako
authors.initials: M
authors.lastname: Miyazawa
authors.forename: Shinobu
authors.initials: S
authors.lastname: Abe
authors.forename: Bunsiti
authors.initials: B
authors.lastname: Simizu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Koike
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004198:Disease Susceptibility; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D016899:Interferon-beta / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000473:pathology / Q000821:virology*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D051379:Mice; D017955:Poliovirus / Q000187:drug effects* / Q000502:physiology; D014779:Virus Replication
chemical_list: D016898:Interferon-alpha; D016899:Interferon-beta