title: Role of the kallikrein-kinin system in Ang-(1-7)-induced vasodilation in mesenteric arterioles of Wistar rats studied in vivo-in situ.
abstract: Angiotensin-(1-7) [Ang-(1-7)], exerts a variety of actions in the cardiovascular system, with an important effect being vasodilation. In this work, we investigated the relationship between the vasodilatory activity of Ang-(1-7) and the kallikrein-kinin system. Intravital microscopy was used to study the vasodilation caused by Ang-(1-7) in the mesenteric vascular bed of anesthetized Wistar rats. The topical application of Ang-(1-7) caused vasodilation of mesenteric arterioles that was reduced by A-779, JE 049 and peptidase inhibitors (aprotinin, SBTI, PKSI 527, E-64, PMSF). These results indicated that the vasodilation induced by Ang-(1-7) in the mesenteric arterioles of Wistar rats was heavily dependent on the activation of kallikrein and subsequent kinin formation.
authors.affiliation: Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes 1524, Cidade Universitária, 05508-900 São Paulo, SP, Brazil.
authors.forename: Rossana Anderson
authors.initials: RA
authors.lastname: Marangoni
authors.forename: Adriana Karaoglanovic
authors.initials: AK
authors.lastname: Carmona
authors.forename: Rita Cássia A Tostes
authors.initials: RC
authors.lastname: Passaglia
authors.forename: Dorothy
authors.initials: D
authors.lastname: Nigro
authors.forename: Zuleica Bruno
authors.initials: ZB
authors.lastname: Fortes
authors.forename: Maria Helena Catelli
authors.initials: MH
authors.lastname: de Carvalho
mesh_terms: D000818:Animals; D007611:Aprotinin / Q000737:chemistry / Q000378:metabolism; D001160:Arterioles / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry*; D016234:Kallikrein-Kinin System; D007610:Kallikreins / Q000737:chemistry*; D008297:Male; D010447:Peptide Hydrolases / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012694:Serine / Q000737:chemistry*; D014664:Vasodilation
chemical_list: D012694:Serine; D007611:Aprotinin; D010447:Peptide Hydrolases; D007610:Kallikreins; D003545:Cysteine