title: Determination of scutellarin in mouse plasma and different tissues by high-performance liquid chromatography.
abstract: A simple HPLC-UV method was developed for the determination of scutellarin in plasma and different tissues of mice (heart, liver, spleen, lungs and kidneys). The separation was achieved by HPLC on a Hypersil C(18) column with a mobile phase composed of methanol-water-glacial acetic acid (40:60:1). UV detection was used at 335 nm. The calibration curves were linear in all matrices (r(2)>0.997) in the concentration range of 0.1-10 microg/ml for plasma and 0.1-20 microg/g for tissue homogenates, respectively. The method described is suitable for studies on the distribution of scutellarin in different tissues of mice.
authors.affiliation: Pharmaceutical Research Institute, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiong
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Ji
authors.initials: J
authors.lastname: Cheng
authors.forename: Jiabi
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D047310:Apigenin / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005965:Glucuronates / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D051379:Mice; D009206:Myocardium / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013154:Spleen / Q000737:chemistry
chemical_list: D005965:Glucuronates; C484876:scutellarin; D047310:Apigenin