Automated, high-resolution cellular retention and uptake studies in vitro.
This report describes an automated method for the measurements of cellular retention and uptake of radiolabeled proteins interacting with cell-surface receptors on intact cancer cells. A complete uptake and retention measurement was performed in one cell dish using a rotating radioimmunoassay (RIA) principle. Compared to common manual measurements, rotating RIA saved both labor time and reagents and provided real-time binding traces with superior time-resolution. The rotating RIA retention profiles for different interactions agreed with retention times reported in the literature.
Björke
Henrik
H
Division of Biomedical Radiation Sciences, Uppsala University, Sweden.
Andersson
Karl
K
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism*; D002277:Carcinoma / Q000000981:diagnostic imaging / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008657:Metabolic Clearance Rate; D011863:Radioimmunoassay / Q000295:instrumentation* / Q000379:methods; D011865:Radioisotope Dilution Technique; D011877:Radionuclide Imaging; D015203:Reproducibility of Results; D012371:Robotics / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity
D000906:Antibodies; D000954:Antigens, Surface