The effects of desferrioxamine and quercetin on liver injury induced by hepatic ischaemia-reperfusion in rats.
BACKGROUND
This study was designed to examine the effects of desferrioxamine and quercetin on hepatic ischaemia-reperfusion injury in rat.


METHODS
Thirty Wistar albino rats were randomized into five groups. Group I was the control group. Group II received no treatment. Group III and group IV received intramuscular injections of desferrioxamine (100 mg/kg per day) and quercetin (50 mg/kg per day) respectively. Group V was administered desferrioxamine and quercetin in combination. After treatment for 3 days, groups II, III, IV, and V were exposed to total hepatic ischaemia for 45 minutes. Plasma alanine aminotransferase levels, malondialdehyde and reduced glutathione activities were measured after reperfusion for 1 hour. Histopathological analysis of liver tissues was carried out.


RESULTS
Our results indicated that tissue malondialdehyde levels and histopathological liver damage scores were significantly higher in the ischaemia-reperfusion group than in the control group. Administration of desferrioxamine, quercetin, and desferrioxamine+quercetin significantly decreased these parameters. Plasma alanine aminotransferase levels were also increased after ischaemia-reperfusion. Quercetin and desferrioxamine + quercetin significantly decreased the activity of this enzyme when compared to ischaemia-reperfusion group.


CONCLUSIONS
The present data suggest that both desferrioxamine and quercetin may be useful to protect against ischaemia-reperfusion induced liver damage.
Tokyol
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C
Department of Pathology, Kocatepe University, Faculty of Medicine, Afyon, Turkey. ctokyol@yahoo.com
Yilmaz
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S
Kahraman
A
A
Cakar
H
H
Polat
C
C
D000818:Animals; D003676:Deferoxamine / Q000494:pharmacology / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology; D008111:Liver Function Tests; D018384:Oxidative Stress / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D017262:Siderophores / Q000494:pharmacology / Q000627:therapeutic use*
D017262:Siderophores; D011794:Quercetin; D003676:Deferoxamine