title: 7,12-dimethylbenz[a]anthracene interferes with the development of cultured mouse mandibular molars.
abstract: Clinical studies suggest that maternal smoking during pregnancy can reduce the crown size of the child's teeth. Delayed dental age compared with chronological age has also been reported in children whose parents smoke. Among the main components of tobacco smoke are nonhalogenated polycyclic aromatic hydrocarbons (PAHs), many of which are highly toxic. Humans are exposed to PAH compounds mainly via tobacco smoke and diet. The aim of our study was to investigate the effect of PAHs on tooth formation and the function of tooth-forming cells. We exposed mouse (NMRI) E18 mandibular first and second molar explants to 7,12-dimethylbenz[a]anthracene (DMBA), a toxic PAH compound, in organ culture for 7 or 12 days. DMBA concentrations used were 0.1, 0.5, 1, and 2 microM. The mesiodistal width of each first molar (12-day culture) was measured in stereomicroscopic images, and the teeth were analysed histologically. DMBA exposure significantly reduced the mesiodistal width of the first molars. DMBA impaired or delayed amelogenesis and dentinogenesis in both molars at the lowest concentration of 0.1 microM. DMBA affected enamel formation more severely than dentin formation and occasionally prevented amelogenesis completely. Elongation and polarization of ameloblasts were impaired, and blood vessel architecture of the dental papilla (future pulp) was altered. Cusps were thin and sharp. In line with the finding that maternal smoking during pregnancy has an adverse effect on child's tooth development, this study shows the toxic influence of PAHs on tooth development in vitro.
authors.affiliation: Department of Pediatric and Preventive Dentistry and Department of Oral Pathology, Institute of Dentistry, 00014 University of Helsinki, FL-00014 Helsinki, Finland. eija.peltonen@helsinki.fi
authors.forename: Eija
authors.initials: E
authors.lastname: Peltonen
authors.forename: Pirjo-Liisa
authors.initials: PL
authors.lastname: Lukinmaa
authors.forename: Carin
authors.initials: C
authors.lastname: Sahlberg
authors.forename: Anna-Maija
authors.initials: AM
authors.lastname: Partanen
authors.forename: Anu
authors.initials: A
authors.lastname: Kiukkonen
authors.forename: Satu
authors.initials: S
authors.lastname: Alaluusua
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000254:growth & development; D002478:Cells, Cultured; D008334:Mandible / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D051379:Mice; D008963:Molar / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development
chemical_list: D015127:9,10-Dimethyl-1,2-benzanthracene