Trichinella spiralis: histamine secretion induced by TSL-1 antigens from unsensitized mast cells.
Mast cells' hyperplasia and activation are prominent features in Trichinella spiralis infection. Recently, it was shown that TSL-1 antigens from T. spiralis muscle larvae induce IL-4 and TNF release by unsensitized, normal mast cells (MC) involving an Ig-independent mechanism. In this study, we characterized histamine secretion induced by TSL-1 antigens from normal, unsensitized rat peritoneal MC. Maximum histamine secretion (30+/-5.3% SEM, n=13) was achieved with 30 ng/mL TSL-1 antigens. However, TSL-1 did not induce an increase in beta-hexosaminidase release or NADPH oxidase activity by MC. Interestingly, histamine secretion by TSL-1 was completed at 10s, and was inhibited by both Bordetella pertussis toxin and neuraminidase V, characteristics similar to those involved in substance P-induced histamine secretion. However, in contrast to substance P, TSL-1 induced histamine secretion in the absence of detectable changes in intracellular Ca(2+). We are investigating the molecular pathways involved in MC activation by TSL-1.
Arizmendi-Puga
Narcy G
NG
Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Alta., Canada.
Enciso
J Antonio
JA
Ortega-Pierres
Guadalupe
G
Zhao
Zhongwei
Z
Duszyk
Marek
M
Ulanova
Marina
M
Befus
A Dean
AD
Yépez-Mulia
Lilián
L
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006636:Histamine Release / Q000276:immunology*; D007202:Indicators and Reagents / Q000378:metabolism; D007814:Larva / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D009132:Muscles / Q000469:parasitology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009439:Neuraminidase / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology; D037342:Pertussis Toxin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016897:Respiratory Burst; D012430:Ruthenium Red / Q000378:metabolism; D013373:Substance P / Q000494:pharmacology; D013997:Time Factors; D017160:Trichinella spiralis / Q000276:immunology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000947:Antigens, Helminth; D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D012430:Ruthenium Red; D013373:Substance P; D037342:Pertussis Toxin; D009439:Neuraminidase; D001619:beta-N-Acetylhexosaminidases; D002118:Calcium; D019331:NG-Nitroarginine Methyl Ester