Identification and characterization of Bombyx mori eIF5A gene through bioinformatics approaches.
As the genome of B. mori is available in GenBank and the EST database of B. mori is expanding, identification of novel genes of B. mori was conceivable by data-mining techniques and bioinformatics tools. In this study, we used the in silico cloning method to identify eukaryotic initiation factor 5A (eIF5A) gene in B. mori. With the hypusine formation, eIF5A is involved in the regulation of cell proliferation and apoptosis. Using the computer program MEGA3, we conducted a search for homologs of eIF5A among many eukaryotic species and confirmed that the eIF5A was conserved in all organisms investigated. This gene has been registered in GenBank under the accession number DQ104412.
Hu
ZhiGang
Z
Institute of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China
Chen
KePing
K
Wang
LinLing
L
Yao
Qin
Q
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D019295:Computational Biology; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000235:genetics*; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000235:genetics*; D012984:Software; D000097574:Eukaryotic Translation Initiation Factor 5A
D004247:DNA; D019476:Insect Proteins; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A