[Expression of survivin mRNA in HHT-induced cell apoptosis of hematological malignancy cell lines].
To investigate the expression of survivin mRNA in hematological malignancy cells and its correlation with HHT-induced cell apoptosis.



Hematological malignancy cell lines MUTZ-1, K562, Jurkat, RPMI and HL60 were treated with HHT in vitro. Cell apoptosis was observed by flow cytometry (FCM) and the expression of surviving and XIAP mRNA was evaluated with semi-quantitative RT-PCR.



The expression of survivin mRNA on cell lines was negatively correlated to HHT-induced cell apoptotic rate (r=-0.980, P=0.003). There were no significant differences in XIAP expression among these 5 cell lines.



Survivin could be used as a new marker for drug-sensitivity and a new target for treatment of hematological malignancies.
Bao
Han-ying
HY
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Cai
Zhen
Z
D017209:Apoptosis / Q000187:drug effects*; D018922:HL-60 Cells; D006248:Harringtonines / Q000494:pharmacology*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D020014:K562 Cells; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D009190:Myelodysplastic Syndromes / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D000077022:Survivin; D014407:Tumor Cells, Cultured
C107489:BIRC5 protein, human; D006248:Harringtonines; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000077022:Survivin; C062500:harringtonin