In vivo performance of a sol-gel glass-coated collagen.
Synthetic bioactive materials offer possibilities to repair large tissue defects. It is well known that bioactivity, angiogenesis, and inflammation are key events in implant incorporation. Using glass-coated and glass-free collagen as potential bone graft substitutes, we carried out in vitro bioactivity and an in vivo angiogenesis and inflammation studies. The in vitro study showed bioactivity when the glass-coated samples were left in SBF for 5 days. This was confirmed by FTIR results, which presented P--O vibration bands characteristic of hydroxyapatite close to 1060 cm(-1) and 600 cm(-1). The in vivo response was evaluated following subcutaneous implantation of the biomaterial in the mouse dorsa. Angiogenesis, as determined by hemoglobin content extracted from implants 7 and 14 days after implantation, increased progressively in both glass-coated and glass-free collagen implants. However, vascularization was higher in the glass-coated collagen implants 14 days after implantation (mug Hb per mg wet tissue 6.0 +/- 0.3) compared with the glass-free group (1.6 +/- 0.1). The inflammatory process, determined by the levels of myeloperoxidase and N-acetylglucosaminidase, was similar for both implants. This study shows that glass-coated collagen implants hold osteogenic and angiogenic potential and may be used in clinical conditions requiring improvement of these biological processes.
Andrade
Angela Leão
AL
Department of Chemistry-ICEX, Federal University of Minas Gerais, CP 702, CEP 31270-901, Belo Horizonte, MG, Brazil.
Andrade
Sílvia Passos
SP
Domingues
Rosana Zacarias
RZ
D000818:Animals; D020099:Coated Materials, Biocompatible; D003094:Collagen; D005898:Glass; D007249:Inflammation / Q000503:physiopathology; D008297:Male; D051379:Mice; D009389:Neovascularization, Pathologic / Q000503:physiopathology; D044367:Phase Transition
D020099:Coated Materials, Biocompatible; D003094:Collagen