Application of a novel oscillatory flow micro-bioreactor to the production of gamma-decalactone in a two immiscible liquid phase medium.
A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of gamma-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred tank bioreactor or shake flask). A three-fold increase in gamma-decalactone productivity was obtained by increasing oscillatory mixing intensity from Re(o) approximately 482 to Re(o) approximately 1447. This was presumably related to the effective contribution of the reactor geometry to enhanced mass transfer rates between the two immiscible liquid phases involved in the process by increasing the interfacial area.
Reis
N
N
Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Gonçalves
C N
CN
Aguedo
M
M
Gomes
N
N
Teixeira
J A
JA
Vicente
A A
AA
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D007783:Lactones / Q000378:metabolism*; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D009991:Oscillometry / Q000295:instrumentation / Q000379:methods; D044367:Phase Transition; D012996:Solutions; D025062:Yarrowia / Q000378:metabolism*
D003470:Culture Media; D004655:Emulsions; D007783:Lactones; D012996:Solutions; C109757:decan-4-olide