Protein expression dynamics during replicative senescence of endothelial cells studied by 2-D difference in-gel electrophoresis.
Endothelial senescence contributes to endothelium dysfunctionality and is thereby linked to vascular aging. A dynamic proteomic study on human umbilical vein endothelial cells, isolated from three umbilical cords, was performed. The cells were cultured towards replicative senescence and whole cell lysates were subjected to 2-D difference gel electrophoresis (DIGE). Despite the biological variability of the three independent isolations, a set of proteins was found that showed senescence-dependent expression patterns in all isolations. We focused on those proteins that showed significant changes, with a paired analysis of variance (RM-ANOVA) p-value of < or =0.05. Thirty-five proteins were identified with LC-Fourier transform MS, and functional annotation revealed that endothelial replicative senescence is accompanied by increased cellular stress, protein biosynthesis and reduction in DNA repair and maintenance. Nuclear integrity becomes affected and cytoskeletal structure is also changed. Such important changes in the cell infrastructure might accelerate endothelium dysfunctionality. This study provides biological information that will initiate studies to further unravel endothelial senescence and gain more knowledge about the consequences of this process in the in vivo situation.
Eman
Michael R
MR
Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Regan-Klapisz
Elsa
E
Pinkse
Martijn W H
MW
Koop
Inge M
IM
Haverkamp
Johan
J
Heck
Albert J R
AJ
Verkleij
Arie J
AJ
Post
Jan A
JA
D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D003599:Cytoskeleton / Q000502:physiology; D004260:DNA Repair / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D042783:Endothelial Cells / Q000502:physiology*; D006801:Humans; D018384:Oxidative Stress / Q000502:physiology; D014176:Protein Biosynthesis / Q000502:physiology*; D014471:Umbilical Veins / Q000166:cytology