Anti-complementary activity of ursane-type triterpenoids from Weigela subsessilis.
A new ursane-type triterpenoid, weigelic acid (1), and seven known compounds, ursolic acid (2), ilekudinol A (3), corosolic acid (4), ilekudinol B (5), esculentic acid (6), pomolic acid (7), and asiatic acid (8) were isolated from the leaf and stem of Weigela subsessilis. The structure of the new triterpenoid was established as 1beta,2alpha,3alpha,23-tetrahydroxyurs-12-en-28-oic acid on the basis of spectroscopic analyses. In addition, the isolated compounds were evaluated for their anti-complement activity against the classical pathway of the complement system. Of these, compounds 1-2 and 4-8 exhibited anti-complement activity with IC50 values of 152, 90, 130, 51, 56, 4, and 163 microM, respectively, whereas 3 was inactive. This shows that a carboxylic group of ursane-type triterpenoids seems to play an important role in inhibiting the hemolytic activity of human serum against erythrocytes.
Thuong
Phuong Thien
PT
College of Pharmacy, Chungnam National University, Daejeon, Korea.
Min
Byung-Sun
BS
Jin
WenYi
W
Na
MinKyun
M
Lee
JongPill
J
Seong
RackSeon
R
Lee
Young-Mi
YM
Song
KyungSik
K
Seong
YeonHee
Y
Lee
Hyeong-Kyu
HK
Bae
KiHwan
K
Kang
Sam Sik
SS
D000818:Animals; D003165:Complement System Proteins / Q000502:physiology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012756:Sheep; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000494:pharmacology*
D014315:Triterpenes; D003165:Complement System Proteins