[Introduce Tagsk1 into salt-sensitive callus to improve the capacity of salt-tolerance by micropartical bombardment].
The Tagsk1 (Triticum asetium L. glycogen synthase kinase 1) gene derived from the genome of wheat salt-tolerance mutant RH8706-49 was cloned by PCR. The special primers designed according to full length cDNA sequence of Tagsk1 (AF525086). A binary expression vector pBI121-gsk1 containing Gus and Tagsk1 was constructed. And pBI121-gsk1 was introduced into the callus induced from mature embryos of salt-sensitive wheat H8706-34 and cv. China Spring by particle bombardment. The transformed callus were screened by Kanamycin and 0.5% NaCl. The salt-tolerance callus were obtained, which showed higher ability of salt-tolerance and could diffirentiate roots and buds on the medium containing 0.5% NaCl.
Xu
Tao
T
The College of Biology, Hebei Normal University, Shijiazhuang 050016, China.
Zhao
Bao-Cun
BC
Ge
Rong-Chao
RC
Shen
Yin-Zhu
YZ
Huang
Zhan-Jing
ZJ
D000222:Adaptation, Physiological; D019470:Biolistics; D018744:DNA, Plant / Q000235:genetics; D038341:Glycogen Synthase Kinases / Q000235:genetics*; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D055051:Salt-Tolerant Plants / Q000235:genetics*; D012639:Seeds / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism*; D014170:Transformation, Genetic; D014908:Triticum / Q000201:enzymology / Q000235:genetics* / Q000502:physiology
D018744:DNA, Plant; D010940:Plant Proteins; D012965:Sodium Chloride; D038341:Glycogen Synthase Kinases