The effect of hypoxia on photocytotoxicity of TICS tricarbocyanine dye in vitro.
OBJECTIVE
To evaluate the effect of cell oxygenation on photocytotoxicity of a novel tricarbocyanine indolenine dye covalently bound to glucose (TICS).


METHODS
HeLa cells were incubated with 5 microM TICS, 2 h later irradiated by laser at 740 nm with a light dose of 10 J/cm(2), delivered at a power density of 10, 20, 25 or 30 mW/cm(2), in air or in argon atmosphere, and then scored for viability.


RESULTS
The photocytotoxicity of TICS increased dramatically as the power density was reduced. Under hypoxia TICS-photosensitized cell death was determined but its value was lowered, compared to photoirradiation in the air.


CONCLUSIONS
Photosensitizing effect of TICS is only partially dependent on the oxygenation of tumor cells.
Istomin
Y P
YP
NN Alexandrov Research Institute of Oncology and Medical Radiology, Minsk, Belarus. Istomin@users.med.by
Alexandrova
E N
EN
Zhavrid
E A
EA
Voropay
E S
ES
Samtsov
M P
MP
Kaplevsky
K N
KN
Lugovsky
A P
AP
Lugovsky
A A
AA
D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D010778:Photochemotherapy / Q000379:methods