Decolorization of triphenylmethane, azo, and anthraquinone dyes by a newly isolated Aeromonas hydrophila strain.
A broad-spectrum dye-decolorizing bacterium, strain DN322, was isolated from activated sludge of a textile printing wastewater treatment plant. The strain was characterized and identified as a member of Aeromonas hydrophila based on Gram staining, morphology characters, biochemical tests, and nearly complete sequence analysis of 16S rRNA gene and the gyrase subunit beta gene (gyrB). Strain DN322 decolorized a variety of synthetic dyes, including triphenylmethane, azo, and anthraquinone dyes. For color removal, the most suitable pH and temperature were pH 5.0-10.0 and 25-37 degrees C, respectively. Triphenylmethane dye, e.g., Crystal Violet, Basic Fuchsin, Brilliant Green, and Malachite Green (50 mg l(-1)) were decolorized more than 90% within 10 h under aerobic culture condition and Crystal Violet could be used as sole carbon source and energy source for cell growth. The color removal of triphenylmethane dyes was due to a soluble cytosolic enzyme, and the enzyme was an NADH/NADPH-dependent oxygenase; For azo and anthraquinone dyes, e.g., Acid Amaranth, Great Red GR, Reactive Red KE-3B, and Reactive Brilliant Blue K-GR (50 mg l(-1)) could be decolorized more than 85% within 36 h under anoxic condition. This strain may be useful for bioremediation applications.
Ren
Suizhou
S
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Guo
Jun
J
Zeng
Guoqu
G
Sun
Guoping
G
D016980:Aeromonas hydrophila / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D000880:Anthraquinones / Q000378:metabolism*; D001391:Azo Compounds / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004396:Coloring Agents / Q000378:metabolism*; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D005840:Gentian Violet; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000378:metabolism; D010619:Phenazines; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012722:Sewage / Q000382:microbiology; D013696:Temperature; D014320:Triphenylmethyl Compounds / Q000378:metabolism*; D014871:Water Microbiology
D000880:Anthraquinones; D001391:Azo Compounds; D004396:Coloring Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C035863:Gram's stain; D007220:Industrial Waste; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014320:Triphenylmethyl Compounds; C046945:triphenylmethane; D010105:Oxygenases; D027081:DNA Gyrase; D005840:Gentian Violet