Release of vasopressin during suppression of oestrous cycle in rat by zoladex and hypovolemic challenge.
BACKGROUND
The vasopressin (AVP) response to hypovolemia has been compared in intact and chemically castrated rats. This functional ovariectomy was done to confirm the findings in surgical ovariectomy of how gonadal steroids modulate the release of AVP under hypo-volemic challenge.


METHODS
Twenty female Sprague Dawley rats were checked for oestrous over two consecutive cycles. The ten control rats were given sub-cutaneous puncture only whereas the experimental were given Zoladex implant. On the fifteenth day all the rats were given intra-peitoneal injection of poly-ehylene glycol. All the rats were de-capitated after an hour.


RESULTS
The uterine weight was significantly decreased in experimental group. The plasma AVP level was also significantly decreased in the experimental group. The pituitary AVP level was significantly increased in the experimental group.


CONCLUSIONS
The chemical castration effected the AVP secretion, this proves that the sex steroids modulate the release of AVP secretion inspite of hypo-volemic challenge.
Kundi
Salma Aslam
SA
Department of Physiology, Ayub Medical College Abbottabad. salmakundi@hotmail.com
Wazir
Saleem
S
Afzal
Saleem
S
Afzal
Naeema
N
Qayum
Iftikhar
I
Forsling
Mary L
ML
D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D004971:Estrus / Q000187:drug effects / Q000378:metabolism; D005260:Female; D017273:Goserelin / Q000494:pharmacology*; D020896:Hypovolemia / Q000378:metabolism*; D010052:Ovariectomy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000187:drug effects; D014667:Vasopressins / Q000187:drug effects* / Q000378:metabolism
D018931:Antineoplastic Agents, Hormonal; D017273:Goserelin; D014667:Vasopressins