Effect of pretreatment of salt, copper and temperature on ultraviolet-B-induced antioxidants in diazotrophic cyanobacterium Anabaena doliolum.
Effect of salt, copper, and temperature pretreatments on the UV-B-induced oxidative damage, measured in terms of peroxide and MDA (lipid peroxidation) contents, was studied in the diazotrophic cyanobacterium Anabaena doliolum. To understand the survival strategy enzymatic (superoxide dismutase, catalase, glutathione reductase, and ascorbate peroxidase) and non-enzymatic (glutathione, ascorbate, alpha-tocopherol and carotenoid) antioxidants were studied. Among the various pretreatments salt was found to decrease and copper and temperature pretreatments increased the deleterious effects of UV-B. This study is the first to demonstrate that physical stress (high temperature) enhanced the damaging effect of UV-B more profoundly than chemical stresses (salt and copper).
Srivastava
Ashish Kumar
AK
Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, India.
Bhargava
Poonam
P
Mishra
Yogesh
Y
Shukla
Bideh
B
Rai
Lal Chand
LC
D017033:Anabaena / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000032:analysis; D002338:Carotenoids / Q000032:analysis; D002374:Catalase / Q000032:analysis; D003300:Copper / Q000494:pharmacology*; D005978:Glutathione / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D006358:Hot Temperature; D008054:Lipid Peroxides / Q000032:analysis; D010544:Peroxidases / Q000032:analysis; D010545:Peroxides / Q000032:analysis; D012492:Salts / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000032:analysis; D024502:alpha-Tocopherol / Q000032:analysis
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D008054:Lipid Peroxides; D010545:Peroxides; D012492:Salts; D002338:Carotenoids; D003300:Copper; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D024502:alpha-Tocopherol; D001205:Ascorbic Acid