[With genechip technique to investigate HLA-DQB1 gene polymorphisms in south and north regions of China].
OBJECTIVE
To research and compare HLA-DQB1 gene frequency(GF) and polymorphism distribution between south and north population of Chinese in China.


METHODS
Combining PCR-sequence specific primers(SSP) and sequence specific oligonucleotide probe(SSOP) techniques and DNA Microarray Kit for HLA-DQB1 Low Res Genotyping from Shenzhen Yi-Shengtang Biological LTD. Co. was used to type HLA-DQB1 gene polymorphisms of 700 individuals living in south China and 320 individuals in north China.


RESULTS
We inspected 10 alleles of HLA-DQB1 and got a series of comprehensive and accurate statistic data.


CONCLUSIONS
It is tested that HLA-DQB1*02, 05, 0601, 0602, 0603 gene frequencies are different obviously(P<0.05) between south and north Chinese. And those data will be useful to kinds of research associated with disease relevant and anthropology research.
Wang
Hui
H
Shenzhen Yi-Shengtang Biological LTD. Company, Shenzhen, Guangdong, 518054 P.R.China. wendyrabbit56@163.com
Wu
Da-lin
DL
Shen
A-dong
AD
Chen
Chun-yan
CY
Zhang
Wen
W
Li
Dan
D
Liu
Ya-qin
YQ
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D015345:Oligonucleotide Probes