title: Characterization of pbpA and pbp2 encoding penicillin-binding proteins located on the downstream of clavulanic acid gene cluster in Streptomyces clavuligerus.
abstract: Two genes, pbpA (orf18) and pbp2 (orf19) located on the downstream of clavulanic acid (CA) gene cluster of Streptomyces clavuligerus were cloned into pET-28a(+), and confirmed to encode a family of high molecular-weight penicillin-binding proteins (PBPs). Both genes were amplified from genomic DNA by PCR and expressed in E. coli BL21 (DE3). Hydropathy plots of the proteins revealed a single stretch of hydrophobic amino acids indicating them to be transmembrane proteins. Pbp2 had lower affinity to penicillin G compared to PbpA, and was essential to the cell growth in contrast to PbpA.
authors.affiliation: Institute of Biomolecule Reconstruction, Sun Moon University, 100, Kalsanri, Tangjeonmyun, 336-708, Asansi, Chungnam, Korea.
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishida
authors.forename: Trinh Viet
authors.initials: TV
authors.lastname: Hung
authors.forename: Kwangkyoung
authors.initials: K
authors.lastname: Liou
authors.forename: Hei Chan
authors.initials: HC
authors.lastname: Lee
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: Shin
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
mesh_terms: D000595:Amino Acid Sequence; D019818:Clavulanic Acid / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D046915:Penicillin-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D046915:Penicillin-Binding Proteins; D011994:Recombinant Proteins; D019818:Clavulanic Acid