[Expression, localization and interrelationship of P27kip1 and cyclin D3 in non-Hodgkin's lymphoma].
OBJECTIVE
To investigate the expression, localization and interrelationship of P27(kip1) and cyclin D3 in non-Hodgkin's lymphoma (NHL).


METHODS
The expressions of P27(kip1), cyclin D3 and index Ki-67 was detected in 100 NHL and 20 reactive lymph nodes by immunohistochemical technique. The expression and localization of P27(kip1) and cyclin D3 in 3 NHL cell lines were detected by Western blot, double immunolabelling and laser scanning confocal microscopy, respectively.


RESULTS
In general the expression of P27(kip1) in NHL was lower than in control group, and was negatively related to the tumor aggressiveness and proliferating activity; the expression of cyclin D3 in NHL was higher than in control group, and was positively related to the tumor aggressiveness and proliferating activity. There was a negative correlation between P27(kip1) and cyclin D3. Nevertheless, anomalous high P27(kip1) expression was found in a few NHL tissues with high expression of cyclin D3 and Ki-67. Overexpression and colocalization of P27(kip1) and cyclin D3 was found in Raji cell line.


CONCLUSIONS
Under expression of P27(kip1) and overexpression of cyclin D3 may play a role in the occurrence and development of NHL. Anomalous high P27(kip1) expression and its interaction with cyclin D3 may be another mechanism for tumor genesis of NHL.
Zhang
Dong-mei
DM
Department of Microbiology and Immunology, Nantong University, Nantong 226001, China.
Lu
Jian-xin
JX
Shen
Ai-guo
AG
Chen
Li
L
He
Song
S
Shao
Xiao-yi
XY
Liu
Hai-ou
HO
Cheng
Chun
C
D045744:Cell Line, Tumor; D056743:Cyclin D3; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism*; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
C534696:CCND3 protein, human; D056743:Cyclin D3; D016213:Cyclins; D050760:Cyclin-Dependent Kinase Inhibitor p27