title: [PLAC1/CP1 gene expression and autologous humoral immunity in gastric cancer patients].
abstract: OBJECTIVE
  To investigate PLAC1/CP1 as a potential candidate gene in gastric cancer therapy.
  
  
  METHODS
  Reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the expression of PLAC1/CP1 mRNA in gastric cancer tissues and paired adjacent non-cancerous tissues resected from 28 patients with gastric cancer. Specific antibodies against PLAC1/CP1 were also detected by ELISA method in gastric cancer patients.
  
  
  RESULTS
  Fourteen (50%,14/28) out of the 28 gastric cancer samples were PLAC1/CP1 mRNA positive. Of the 28 serum samples tested,eight (29%,8/28) displayed positive seroreactivity against PLAC1/CP1 antigen, accounting for 57% (8/14) of PLAC1/CP1 mRNA positive samples.
  
  
  CONCLUSIONS
  PLAC1/CP1 mRNA was expressed in high frequency and induced spontaneous antibody responses in gastric cancer patients. PLAC1/CP1 may be a valuable candidate antigen in gastric cancer immunotherapy.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100083, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Xue-wen
authors.initials: XW
authors.lastname: Pang
authors.forename: Fang-fang
authors.initials: FF
authors.lastname: Liu
authors.forename: Xue-yuan
authors.initials: XY
authors.lastname: Dong
authors.forename: Hong-cheng
authors.initials: HC
authors.lastname: Wang
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei-feng
authors.initials: WF
authors.lastname: Chen
mesh_terms: D000912:Antibodies, Neoplasm / Q000276:immunology; D005260:Female; D015870:Gene Expression; D006801:Humans; D056724:Immunity, Humoral; D008297:Male; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000912:Antibodies, Neoplasm; C415583:PLAC1 protein, human; D011257:Pregnancy Proteins