title: Histological changes of the testis and epididymis in adult rats as a result of Leydig cell destruction after ethane dimethane sulfonate treatment: a morphometric study.
abstract: OBJECTIVE
  To quantitatively study the histological changes of the testis and epididymis as a result of a drastic reduction of testosterone secretion.
  
  
  METHODS
  Fourteen adult Sprague-Dawley rats were injected intraperitoneally with ethane dimethane sulfonate (EDS, 75 mg/kg) and the same number of animals were injected with normal saline as a control. At days 7 and 12 (after treatment), respectively, half of the animals from each group were killed. The testes and epididymides were removed and tissue blocks embedded in methacrylate resin. The cell number per testis was estimated using the stereological optical disector and some other parameters were obtained using other morphometric methods.
  
  
  RESULTS
  The EDS treatment resulted in an almost complete elimination of Leydig cells but had no effect on the numbers of Sertoli cells per testis. At day 7 after EDS treatment, many elongated spermatids were retained in the seminiferous epithelium and many round spermatids could be seen in the epididymal ducts. At day 12, a looser arrangement of spermatids and spermatocytes became evident, with apparent narrow empty spaces being formed between germ cells in an approximately radial direction towards the tubule lumen; the numbers (per testis) of non-type B spermatogonia and spermatocytes were similar to controls, whereas that of type B spermatogonia increased by 59%, and that of early round, elongating and late elongated spermatids decreased by 37%, 72% and 52%, respectively.
  
  
  CONCLUSIONS
  The primary spermatogenic lesions following EDS administration were (i) spermiation failure and (ii) detachment of spermatids and spermatocytes associated with impairment in spermiogenesis and meiosis.
authors.affiliation: Morphometric Research Laboratory, North Sichuan Medical College, 234 Fujiang Road, Nanchong, Sichuan 637007, China. zwyang@mail.nctele.com
authors.forename: Zheng-Wei
authors.initials: ZW
authors.lastname: Yang
authors.forename: Ling-Shu
authors.initials: LS
authors.lastname: Kong
authors.forename: Yang
authors.initials: Y
authors.lastname: Guo
authors.forename: Jin-Qi
authors.initials: JQ
authors.lastname: Yin
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Mills
mesh_terms: D000818:Animals; D004822:Epididymis / Q000187:drug effects / Q000473:pathology*; D007274:Injections, Intraperitoneal; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology*; D008297:Male; D008698:Mesylates / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012671:Seminiferous Tubules / Q000473:pathology; D013737:Testis / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000473:pathology
chemical_list: D008698:Mesylates; C002994:ethylene dimethanesulfonate