Identification of Candida dubliniensis in a diagnostic microbiology laboratory.
Candida dubliniensis is an emerging yeast pathogen isolated mainly from immunocompromised patients. As molecular tests are currently unsuitable for use in routine diagnostic laboratories, we compared a variety of phenotypic techniques for differentiating C. albicans and C. dubliniensis. The tests included: colony colour on CHROMagar Candida medium; growth at 37 degrees C and 45 degrees C; ability to produce germ tubes and chlamydospores; and the Auxacolor system. The organisms included 105 isolates previously identified as C. albicans, 10 reference strains of C. albicans, 2 reference strains of C. dubliniensis and 102 fresh clinical isolates identified as C. albicans. None of the tests alone was satisfactory but a combination of 3 tests may be suitable for presumptive identification of C. dubliniensis.
Momani
O M
OM
Department of Microbiology, Omar Medical Laboratories, Amman, Jordan. momaniomar@hotmail.com
Qaddoomi
A
A
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D000362:Agar; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D002176:Candida albicans / Q000145:classification / Q000235:genetics; D002177:Candidiasis / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology*; D002863:Chromogenic Compounds; D003116:Color; D021821:Communicable Diseases, Emerging / Q000382:microbiology; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods* / Q000592:standards; D004271:DNA, Fungal / Q000235:genetics; D003937:Diagnosis, Differential; D025141:Drug Resistance, Fungal; D006801:Humans; D016867:Immunocompromised Host; D016533:Mycological Typing Techniques / Q000379:methods* / Q000592:standards; D010641:Phenotype; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors
D002863:Chromogenic Compounds; D003470:Culture Media; D004271:DNA, Fungal; D000362:Agar