[Effect of gamma-aminobutyric acid B receptor on nitric oxide/nitric oxide synthase system during recurrent febrile seizures].
OBJECTIVE
To explore the effect of gamma-aminobutyric acid B receptor (GABA(B)R)on nitric oxide (NO)/nitric oxide synthase (NOS) system during recurrent febrile seizures (FS).


METHODS
Sprague-Dawley rats aged 21 days were randomly divided into four groups:control group, FS group, FS + baclofen group, FS + phaclofen group. FS in rats were induced ten times in a bath of warm water,once every 2 days. The plasma level of NO was detected by the spectrophotometer; the expression of nNOS mRNA was examined by in situ hybridization; the expression of nNOS protein was observed by immunohistochemistry.


RESULTS
Compared with those in FS group,the plasma level of NO decreased [(19.02+/-9.31) micromol/L vs (40.03+/-9.12)micromol/L], and the expression of nNOS was down-regulated in FS + baclofen group; the plasma level of NO increased [(66.46+/-8.15) micromol/L vs (40.03+/-9.12) micromol/L] and the expression of nNOS was up-regulated in FS + phaclofen group.


CONCLUSIONS
GABA(B)R modulated the expression of NO/NOS system during recurrent FS.
Han
Ying
Y
Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.
Qin
Jiong
J
Bu
Ding-fang
DF
Yang
Zhi-xian
ZX
Chang
Xing-zhi
XZ
Du
Jun-bao
JB
D000818:Animals; D009569:Nitric Oxide / Q000097:blood*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000378:metabolism*; D003294:Seizures, Febrile / Q000378:metabolism*
D012333:RNA, Messenger; D018080:Receptors, GABA-B; D009569:Nitric Oxide; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat