Proteomic analysis of liver tissues subjected to early ischemia/reperfusion injury during human orthotopic liver transplantation.
Knowledge of early molecular events occurring upon ischemia/reperfusion (I/R) during liver transplantation (LT) is of great importance to improve the therapeutic intervention of surgical treatment. However, nowadays, few data are available on early protein targets of I/R injury. To identify these proteins, we used a differential proteomics approach in the characterization human liver biopsies during I/R upon LT. Analyses were performed on nine donor livers during LT. By using 2-DE and MALDI-TOF MS, we identified 36 proteins which resulted significantly altered upon I/R injury. The majority of these proteins are functionally involved in lipid and energy metabolism, in different metabolic pathways, in redox signalling and in oxidative-stress response. Our data represent the first global approach in the study of I/R injury in liver.
Vascotto
Carlo
C
Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy.
Cesaratto
Laura
L
D'Ambrosio
Chiara
C
Scaloni
Andrea
A
Avellini
Claudio
C
Paron
Igor
I
Baccarani
Umberto
U
Adani
Gian Luigi
GL
Tiribelli
Claudio
C
Quadrifoglio
Franco
F
Tell
Gianluca
G
D000328:Adult; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008099:Liver / Q000098:blood supply / Q000737:chemistry* / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D020543:Proteome / Q000032:analysis*; D015427:Reperfusion Injury / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome