Use of a short fragment of the C-terminal E gene for detection and characterization of two new lineages of dengue virus 1 in India.
Here we propose the use of a 216-nucleotide fragment located in the carboxyl terminus of the E gene (E-COOH) and a pairwise-based comparison method for genotyping of dengue virus 1 (DENV-1) strains. We have applied this method to the detection and characterization of DENV-1 in serum samples from travelers returning from the tropics. The results obtained with the typing system correlate with the results obtained by comparison of the sequences of the entire E gene of the strains. The approach demonstrates utility in plotting the distribution and circulation of different genotypes of DENV-1 and also suggests the presence of two new clades of Indian strains. The integration of the method with an online database and a typing characterization tool enhances its strength. Additionally, the analysis of the complete E gene of DENV-1 strains suggested the occurrence of a nondescribed recombination event in the China GD23-95 strain. We propose the use of this methodology as a tool for real-time epidemiological surveillance of dengue virus infections and their pathogenesis.
Domingo
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Laboratorio de Arbovirus y Enfermedades Víricas Importadas, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo Km 2, Majadahonda (28220), Madrid, Spain. domingoc@isciii.es
Palacios
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Jabado
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Reyes
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Niedrig
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Gascón
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Cabrerizo
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Lipkin
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Tenorio
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D004279:DNA, Viral / Q000032:analysis*; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D016679:Genome, Viral; D006801:Humans; D007194:India / Q000453:epidemiology; D017720:Molecular Epidemiology / Q000379:methods*; D010802:Phylogeny
D004279:DNA, Viral