title: Adjuvant effect of the Propionibacterium acnes and its purified soluble polysaccharide on the immunization with plasmidial DNA containing a Trypanosoma cruzi gene.
abstract: In the present work we investigated the role of killed Propionibacterium acnes or a soluble polysaccharide extracted from bacterium cell wall in modulated experimental immunization with plasmidial DNA. We used a plasmid, p154/13, containing a gene-encoding catalytic domain of Trypanosoma cruzi (T. cruzi) trans-sialidase. As previously described, immunization of BALB/c mice with p154/13 elicited humoral, cell-mediated and protective immune responses against T. cruzi infection. In this study we describe that both P. acnes and its soluble polysaccharide fraction have the ability to modulate the immune response elicited by p154/13. Treatment with these adjuvants enhanced specific trans-sialidase Th1 immune response, as revealed by a lower IgG1/IgG2a ratio and stronger in vitro IFN-gamma synthesis by CD4+ T cells. The most important fact was that treatment with P. acnes or its soluble polysaccharide fraction in the presence of p154/13 significantly reduced the peak of parasitemia observed 7 to 8 days after T. cruzi challenge. These data suggest that P. acnes or its soluble polysaccharide fraction may improve the protective potential of a DNA vaccine against experimental T. cruzi infection.
authors.affiliation: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo -Escola Paulista de Medicina, São Paulo, Brasil.
authors.forename: Juliana Sekeres
authors.initials: JS
authors.lastname: Mussalem
authors.forename: José Ronnie C
authors.initials: JR
authors.lastname: Vasconcelos
authors.forename: Carla Cristina
authors.initials: CC
authors.lastname: Squaiella
authors.forename: Renata Zeigler
authors.initials: RZ
authors.lastname: Ananias
authors.forename: Eleni Gonçalves
authors.initials: EG
authors.lastname: Braga
authors.forename: Maurício M
authors.initials: MM
authors.lastname: Rodrigues
authors.forename: Ieda Maria
authors.initials: IM
authors.lastname: Longo-Maugéri
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D014355:Chagas Disease / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology; D018512:Parasitemia / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D011134:Polysaccharides / Q000276:immunology / Q000302:isolation & purification; D011425:Propionibacterium acnes / Q000737:chemistry / Q000276:immunology*; D016052:Protozoan Vaccines / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology*; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000276:immunology*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D011134:Polysaccharides; D016052:Protozoan Vaccines; D019444:Vaccines, DNA; D007371:Interferon-gamma; D009439:Neuraminidase