title: Evaluation of phenolic compounds in virgin olive oil by direct injection in high-performance liquid chromatography with fluorometric detection.
abstract: Hydrophilic phenols are the most abundant natural antioxidants of virgin olive oil (VOO), in which tocopherols and carotenes are also present. The prevalent classes of hydrophilic phenols found in VOO are phenyl alcohols, phenolic acids, secoiridoids such as the dialdehydic form of decarboxymethyl elenolic acid linked to (3,4-dihydroxyphenyl)ethanol or (p-hydroxypheny1)ethanol (3,4-DHPEA-EDA or p-HPEA-EDA) and an isomer of the oleuropein aglycon (3,4-DHPEA-EA), lignans such as (+)-1-acetoxypinoresinol and (+)-pinoresinol, and flavonoids. A new method for the analysis of VOO hydrophilic phenols by direct injection in high-performance liquid chromatography (HPLC) with the use of a fluorescence detector (FLD) has been proposed and compared with the traditional liquid-liquid extraction technique followed by the HPLC analysis utilizing a diode array detector (DAD) and a FLD. Results show that the most important classes of phenolic compounds occurring in VOO can be evaluated using HPLC direct injection. The efficiency of the new method, as compared to the liquid-liquid extraction, was higher to quantify phenyl alcohols, lignans, and 3,4-DHPEA-EA and lower for the evaluation of 3,4-DHPEA-EDA and p-HPEA-EDA.
authors.affiliation: Dipartimento di Scienze Economico, Estimative e degli Alimenti, Sezione di Tecnologie e Biotecnologie degli Alimenti, Università degli Studi di Perugia, Via S. Costanzo, 06126 Perugia, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Selvaggini
authors.forename: Maurizio
authors.initials: M
authors.lastname: Servili
authors.forename: Stefania
authors.initials: S
authors.lastname: Urbani
authors.forename: Sonia
authors.initials: S
authors.lastname: Esposto
authors.forename: Agnese
authors.initials: A
authors.lastname: Taticchi
authors.forename: GianFrancesco
authors.initials: G
authors.lastname: Montedoro
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D039823:Iridoids; D017705:Lignans; D000069463:Olive Oil; D010636:Phenols / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000975:Antioxidants; D039823:Iridoids; D017705:Lignans; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils