title: Identification of CD36 as a new surface marker of marginal zone B cells by transcriptomic analysis.
abstract: Follicular (FO) B cells and marginal zone (MZ) B cells belong to the mature B cell population in spleen of mice. To identify new surface markers of these mature B cell subsets, we compared gene expression profiles of FO and MZ B cells by DNA microarray using FACS-sorted mouse FO and MZ B cells. From 14,000 mouse genes, 27 membrane proteins were expressed mainly in MZ B cells while another 22 membrane proteins expressed largely in FO B cells. Using FACS analysis, we identified that CD36, CD68, and CD49e were expressed on MZ B cells but not on FO B cells. In addition, using semi-quantitative PCR, we found that the mRNA of CD131 were much more abundant in MZ B cells. These results revealed new phenotypic properties of MZ and FO B cells, and would facilitate further studies in the differentiation and functions of these mature B cells.
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, and Department of Dermatology, Xijng Hospital, Xi'an 710032, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yaochun
authors.initials: Y
authors.lastname: Wang
authors.forename: Lihong
authors.initials: L
authors.lastname: Hou
authors.forename: Ying
authors.initials: Y
authors.lastname: Xing
authors.forename: Hongyan
authors.initials: H
authors.lastname: Qin
authors.forename: Jishu
authors.initials: J
authors.lastname: Wang
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Liang
authors.forename: Hua
authors.initials: H
authors.lastname: Han
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D015415:Biomarkers; D018955:CD36 Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D018955:CD36 Antigens