[Purification of a new phospholipase A2 homologue from Agkistrodon blomhoffii siniticus and its effects on gene expression of Hep3B cells].
A new PLA2 homologue was purified from Agkistrodon blomhoffii Siniticus by applying reverse phase (HPLC) C18 column. The molecular weight of PLA2 homologue is 13900Da and its purity is 97.2%. The results of N-terminal sequence analysis were HLLQFRKMIKKMTKK. All the fragments were determined with the protein-protein BLAST software of GenBank. BLAST analysis showed that the N-terminal sequences of 15 amino acids were highly homologous with the sequences of other PLA2.
Ma
An-de
AD
Instrumental Analysis and Research Center Southern Medical University, Guangzhou 510515, China.
Wu
Shao-yu
SY
Zhang
Jia-jie
JJ
Li
Zhi-qin
ZQ
Xu
Wei
W
Yu
Le
L
Wen
Xiao-yun
XY
Wu
Shu-guang
SG
D017836:Agkistrodon; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010741:Phospholipases A / Q000737:chemistry / Q000302:isolation & purification*; D054467:Phospholipases A2; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D012910:Snake Venoms / Q000737:chemistry*
D012910:Snake Venoms; D010741:Phospholipases A; D054467:Phospholipases A2