Fast simultaneous determination of urinary 1-hydroxypyrene and 3-hydroxybenzo[a]pyrene by liquid chromatography-tandem mass spectrometry.
A fast analysis method using liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry was developed for the simultaneous determination of the 1-hydroxypyrene (1-OHP) and 3-hydroxybenzo[a]pyrene (3-OHBaP) in urine. Mass transitions were monitored at m/z 219.3-200.0 for 1-OHP and m/z 269.2-252.2 for 3-OHBaP. Only 10 min was needed for the analysis. The recovery was 60% for 3-OHBaP and 91% for 1-OHP, respectively. And the method detection limits were 0.49 microg/L for 1-OHP and 1.03 microg/L for 3-OHBaP. The inter- and intra-day relative standard deviations were in the range of 2.8-8.9% for 1-OHP and 9.7-20.8% for 3-OHBaP, respectively. The developed method was successfully used to measure urinary PAH metabolites of student volunteers in a high school.
Fan
Ruifang
R
Guangdong Key Laboratory of Environmental Protection & Resource Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR China.
Dong
Yulian
Y
Zhang
Wenbing
W
Wang
Yu
Y
Yu
Zhiqiang
Z
Sheng
Guoying
G
Fu
Jiamo
J
D000293:Adolescent; D001580:Benzopyrenes / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011721:Pyrenes / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016482:Urinalysis
D001580:Benzopyrenes; D011721:Pyrenes; C021338:3-hydroxybenzo(a)pyrene; C033146:1-hydroxypyrene