title: Dehydroepiandrosterone sulphate sulphohydrolase [correction of sulphoydrolase] from human placenta microsomes--properties of the purified enzyme.
abstract: A form of steroid sulphate sulphohydrolase (EC 3.1.6.2) hydrolysing the dehydroepiandrosterone sulphate (DHEAS-ase) was purified from human placenta microsomes. During the purification procedure the DHEAS-ase was separated from the oestrone sulphate sulphohydrolase (OS-ase). The purified DHEAS-ase revealed specific activity of 1520 nmolxmin-1xmgprotein-1 and exhibited optimal activity at pH 8.4. The Km value was established to be 3.3+/-0.07x10(-5) M. The pI value was around 8.7. The molecular weight estimated by gel filtration was 7.4 kDa. The purified DHEAS-ase was not sensitive to the common sulphohydrolase inhibitors, such as phosphate, sulphate and sulphide ions, but was inhibited by several phosphohydrolase inhibitors (ammonium molybdate, vanadium oxide(V), zinc acetate). Steroids effected inhibition or activation of the purified enzyme. The data concerning substances reacting with -SH groups suggest that in the physiological conditions DHEAS-ase is controlled by the redox status of the cell.
authors.affiliation: Mikołaj Kopernik University, Institute of Biology and Molecular Biology, Biochemistry Department, 87-100 Toruń, ul. Gagarina 7/9, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Niewiadomska
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Gniot-Szulzycka
mesh_terms: D004791:Enzyme Inhibitors / Q000737:chemistry; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008861:Microsomes / Q000201:enzymology*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000502:physiology; D043266:Steryl-Sulfatase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D004791:Enzyme Inhibitors; D011257:Pregnancy Proteins; D043266:Steryl-Sulfatase