Cold-inducible RNA binding protein is required for the expression of adhesion molecules and embryonic cell movement in Xenopus laevis.
We have previously shown that the Xenopus homologue of cold-inducible RNA binding protein, XCIRP-1, is required for the morphogenetic migration of the pronephros during embryonic development. However, the underlying molecular mechanisms remain elusive. Here, we report that XCIRP is essential for embryonic cell movement, as suppression of XCIRP by microinjection of anti-sense mRNA and morpholino antisense oligonucleotides (MOs) significantly reduced protein expression, inhibited the cell migration rate, and inhibited eFGF and activin-induced animal cap elongation. By immunoprecipitation and RT-PCR, we further showed that the mRNA of a panel of adhesion molecules, including alphaE- and beta-catenin, C- and E-cadherin, and paraxial proto-cadherin, are the targets of XCIRP. Consistently, in animal cap explant studies, suppression of XCIRP by MOs inhibited the expression of these adhesion molecules, while over-expression of sense XCIRP-1 mRNA fully rescued this inhibition. Taken together, these results suggest for the first time that XCIRP is required to maintain the expression of adhesion molecules and cell movement during embryonic development.
Peng
Ying
Y
Department of Neurology, The Second Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
Yang
Pai-Hao
PH
Tanner
Julian A
JA
Huang
Jian-Dong
JD
Li
Ming
M
Lee
Henry F
HF
Xu
Ren-He
RH
Kung
Hsiang-Fu
HF
Lin
Marie C M
MC
D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D019070:Cell Lineage / Q000235:genetics; D002465:Cell Movement / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D016372:RNA, Antisense / Q000235:genetics / Q000494:pharmacology; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D029867:Xenopus Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism
C112949:CIRBP protein, Xenopus; D015815:Cell Adhesion Molecules; D009419:Nerve Tissue Proteins; D016372:RNA, Antisense; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins