title: Genotyping of Ureaplasma diversum isolates using pulsed-field electrophoresis.
abstract: Ureaplasma diversum has been associated with reproductive disorders in cattle and in the present study genotypic variations among U. diversum isolates obtained from the vaginal mucus of healthy cattle and sick animals were analyzed by enzymatic digestion and pulsed-field gel electrophoresis (PFGE). The influence of time and broth volume was important in obtaining sufficient cell sediment and DNA for PFGE. The method presented a high discriminatory power and satisfactory reproducibility for the analysis of detected variations among U. diversum isolates and strains. Different band profiles and wide genotypic heterogeneity were detected but no association between DNA polymorphism and sick or healthy animals could be established.
authors.affiliation: Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de São Paulo, Rua Professor Lineu Prestes, 1374 São Paulo, Brazil.
authors.forename: Melissa
authors.initials: M
authors.lastname: Buzinhani
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Buim
authors.forename: Maurício
authors.initials: M
authors.lastname: Yamaguti
authors.forename: Rosângela C
authors.initials: RC
authors.lastname: Oliveira
authors.forename: Elena
authors.initials: E
authors.lastname: Mettifogo
authors.forename: Jorge
authors.initials: J
authors.lastname: Timenetsky
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005260:Female; D005838:Genotype; D011110:Polymorphism, Genetic; D014509:Ureaplasma / Q000145:classification / Q000235:genetics*; D016869:Ureaplasma Infections / Q000382:microbiology / Q000662:veterinary*
chemical_list: D004269:DNA, Bacterial