HGF-induced capillary morphogenesis of endothelial cells is regulated by Src.
The signal transduction pathway involved in hepatocyte growth factor (HGF)-induced capillary morphogenesis of endothelial cells was investigated. HGF-induced capillary morphogenesis of the murine spleen endothelial cell line MSS31 was inhibited by a Src family kinase inhibitor, PP2. Stable expression of kinase-inactive Src in MSS31 cells inhibited HGF-induced activation of Src as well as capillary morphogenesis. The HGF-induced capillary morphogenesis of human umbilical vein endothelial cells was also inhibited by PP2 and was reduced by the downregulation of Src by small interfering RNA. These results suggest that HGF induces capillary morphogenesis of endothelial cells through Src.
Kanda
Shigeru
S
Department of Molecular Microbiology and Immunology, Division of Endothelial Cell Biology, Nagasaki, Japan. skanda-jua@umin.net
Kanetake
Hiroshi
H
Miyata
Yasuyoshi
Y
D000818:Animals; D002196:Capillaries / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D017228:Hepatocyte Growth Factor / Q000008:administration & dosage*; D051379:Mice; D009024:Morphogenesis / Q000187:drug effects / Q000502:physiology*; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D019061:src-Family Kinases / Q000378:metabolism*
D017228:Hepatocyte Growth Factor; D019061:src-Family Kinases