Low viscous separation media for genomics and proteomics analysis on microchip electrophoresis system.
Microchip electrophoresis has widely grown during the past few years, and it has showed a significant result as a strong separation tool for genomic as well as proteomic researches. To enhance and expand the role of microchip electrophoresis, several studies have been proposed especially for the low viscous separation media, which is an important factor for the success of microchip with its narrow separation channels. In this paper we show an overview for the done researches in the field of low viscous media developed for the use in microchip electrophoresis. For genomic separation studies polyhydroxy additives have been used enhance the separation of DNA at low polymer concentration of HPMC (Hydroxypropylmethyl cellulose) which could keep the viscosity low. Mixtures of poly(ethylene oxide) as well as Hydroxyporpyl cellulose have been successfully introduced for chip separation. Furthermore high molecular mass polyacrylamides at low concentrations have been studied for DNA separation. A mixture of polymer nanoparticle with conventional polymers could show a better resolution for DNA at low concentration of the polymer. For the proteomic field isoelectric focusing on chip has been well overviewed since it is the most viscous separation media which is well used for the protein separation. The different types of isoelectric focusing such as the ampholyte-free type, the thermal type as well as the ampholyte-depended type have been introduced in this paper. Isoelectric focusing on chip with its combination with sodium dodecyl sulfate (SDS) page or free solution could give a better separation. Several application for this low viscous separation medias for either genomic or proteomic could clearly show the importance of this field.
Jabasini
Mohammad
M
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Japan. jabasini@mail.apchem.nagoya-u.ac.jp
Murakami
Yuji
Y
Kaji
Noritada
N
Tokeshi
Manabu
M
Baba
Yoshinobu
Y
D000818:Animals; D004247:DNA / Q000302:isolation & purification; D004586:Electrophoresis / Q000295:instrumentation*; D005079:Excipients; D023281:Genomics / Q000295:instrumentation*; D006801:Humans; D007202:Indicators and Reagents; D008838:Microcomputers; D011506:Proteins / Q000302:isolation & purification; D040901:Proteomics / Q000295:instrumentation*
D005079:Excipients; D007202:Indicators and Reagents; D011506:Proteins; D004247:DNA