Follicle-stimulating hormone (FSH) stimulates the expression of Pin1, a peptidyl-prolyl isomerase, in the bovine granulosa cells.
A peptidyl-prolyl isomerase, Pin 1, has been shown to play a role in the regulation of cell cycle progression, both in vitro and in vivo. However, the involvement of Pin 1 during follicular development is not well understood. The aim of this study was first to investigate the expression of Pin 1 mRNA in the granulosa and theca cells of the follicle at different developmental stages of follicles in the bovine ovary, and second, to examine the effects of follicle-stimulating hormone (FSH) and estradiol (E2) on the expression of Pin 1 in the cultured bovine granulosa cells. Follicles were classified into four groups based on the diameter (dominant follicles >8.5mm in diameter, subordinate follicles <8.5mm in diameter) and the relative levels of E2 and progesterone (P4) (E2:P4>1, estrogen active; E2:P4<1, estrogen inactive): i.e. preovulatory dominant follicles (POFs); E2 active dominant follicles (EADs); E2 inactive dominant follicles (EIDs); small follicles (SFs). The expression of the Pin 1 gene was significantly increased in the granulosa cells of EADs as compared with those of other follicles, whereas its expression in theca cells did not differ among follicles at different developmental stages. The concentration of 5 ng/ml FSH alone and the combination of 1 ng/ml E2 and 5 ng/ml FSH stimulated the expression of the Pin 1 gene in bovine granulosa cells. Our data provide the first evidence that Pin 1 expression in the granulosa cells but not the theca cells changes during follicular development, and that FSH stimulate the expression of the Pin 1 gene. These results suggest that Pin 1 regulates the timing of cell proliferation and may act as an intracellular signal responder in the granulosa cells during bovine follicle development.
Shimizu
Takashi
T
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine Inada-machi, Obihiro, Hokkaido 0808555, Japan. shimizut@obihiro.ac.jp
Tetsuka
Masafumi
M
Miyamoto
Akio
A
Uchida
Takafumi
T
D000818:Animals; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006107:Granulosa Cells / Q000166:cytology / Q000201:enzymology*; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development; D019696:Peptidylprolyl Isomerase / Q000235:genetics*; D012333:RNA, Messenger; D013799:Theca Cells / Q000201:enzymology
D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D012333:RNA, Messenger; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D019696:Peptidylprolyl Isomerase