title: Phospholipase D couples survival and migration signals in stress response of human cancer cells.
abstract: MDA-MB-231 human breast cancer cells belong to a highly invasive metastatic cell line that depends on phospholipase D (PLD) activity for survival when deprived of serum growth factors. In response to the stress of serum withdrawal, there is a rapid and dramatic increase in PLD activity. Concomitant with increased PLD activity, there was an increase in the ability of MDA-MB-231 cells to both migrate and invade Matrigel. The ability of MDA-MB-231 cells to both migrate and invade Matrigel was dependent on both PLD and mTOR, a downstream target of PLD signals. Serum withdrawal also led to a PLD-dependent increase in the expression of the stress factor, hypoxia-inducible factor-1alpha. These data reveal that PLD survival signals not only prevent apoptosis but also stimulate cell migration and invasion, linking the ability to suppress apoptosis with the ability to metastasize.
authors.affiliation: Department of Biological Sciences, Hunter College of the City University of New York, New York, New York 10021, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Vanessa
authors.initials: V
authors.lastname: Rodrik
authors.forename: Alfredo
authors.initials: A
authors.lastname: Toschi
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.forename: Li
authors.initials: L
authors.lastname: Hui
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Shen
authors.forename: David A
authors.initials: DA
authors.lastname: Foster
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D002470:Cell Survival; D016895:Culture Media, Serum-Free; D006801:Humans; D009362:Neoplasm Metastasis; D018384:Oxidative Stress; D010739:Phospholipase D / Q000378:metabolism*
chemical_list: D016895:Culture Media, Serum-Free; D010739:Phospholipase D