title: Regulation of human neurotropic JCV in colon cancer cells.
abstract: BACKGROUND
  Recently, the genome of the human polyomavirus, JC (JCV), and expression of its early and late regulatory proteins (T-antigen and agnoprotein) have been demonstrated in neoplastic cells of colonic cancer cases.
  
  
  METHODS
  Regulation of JCV was investigated in a human colon cancer cell line (SW480) and compared to a human glioblastoma cell line (U87-MG) that is permissive for JCV replication.
  
  
  RESULTS
  SW480 cells supported basal transcription of both early and late JCV promoters. The expression of TCF-4, a component of Wnt signaling, modulated JCV transcription in a cell type-specific manner. Both TCF-4 and T-antigen bound to the JCV promoter region and bound to each other. In addition, the expression of TCF-4 caused a decrease in the ability of the T-antigen to stimulate viral DNA replication in U87-MG cells.
  
  
  CONCLUSIONS
  Wnt pathway signaling proteins and T-antigen interact to regulate JCV in colonic epithelial cells.
authors.affiliation: Center for Neurovirology, Department of Neuroscience, Temple University School of Medicine, Philadelphia, PA 19122, USA.
authors.forename: Sahnila
authors.initials: S
authors.lastname: Enam
authors.forename: Dai-Di
authors.initials: DD
authors.lastname: Gan
authors.forename: Martyn K
authors.initials: MK
authors.lastname: White
authors.forename: Luis
authors.initials: L
authors.lastname: Del Valle
authors.forename: Kamel
authors.initials: K
authors.lastname: Khalili
mesh_terms: D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D005909:Glioblastoma / Q000235:genetics / Q000821:virology; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D011401:Promoter Regions, Genetic; D015398:Signal Transduction; D051820:TCF Transcription Factors / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic; D014162:Transfection; D014779:Virus Replication; D051153:Wnt Proteins / Q000235:genetics / Q000502:physiology
chemical_list: D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D051820:TCF Transcription Factors; D051153:Wnt Proteins