title: Anti type I allergic property of Japanese butterbur extract and its mast cell degranulation inhibitory ingredients.
abstract: Pollenosis is a disease that affects 1 in 10 of the Japanese population. During the season of cedar pollen dispersal, many patients suffer from symptoms such as sniffling, sternutation, and itching of the eyes. Japanese butterbur is a popular vegetable and is one of the few domestic vegetables in Japan. The anti type I allergic effects of an aqueous ethanol extract from aerial parts of Japanese butterbur (JBE) were evaluated in rats and RBL-2H3 mast cells. In the passive cutaneous anaphylaxis reaction in rats, a single oral treatment of JBE (1000 mg/kg) was found to suppress the reaction. In IgE-sensitized RBL-2H3 cells, JBE (10-100 microg/mL) inhibited beta-hexosaminidase release, leukotriene C(4)/D(4)/E(4) synthesis, and TNF-alpha production. Moreover, a high concentration of JBE (1000 microg/mL) suppressed smooth muscle constriction induced by histamine (10 microM) and leukotriene D(4) (10 nM) in a guinea pig trachea strip. The search for components in JBE with an inhibitory activity on mast cell degranulation was guided by inhibition of beta-hexsosaminidase release. Two eremophilane-type sesquiterpenes, six polyphenolic compounds, and two triterpene glycosides were isolated. Of these compounds, fukinolic acid, a principal polyphenol constituent, showed potent inhibitory activity (IC(50) value = 2.1 microg/mL). Consequently, On the basis of its inhibition of mast cell activation and direct smooth muscle reaction induced by released mediators, JBE was found to suppress the type I allergic reaction.
authors.affiliation: Research and Development Division, Oryza Oil & Fat Chemical Company, Ltd., Ichinomiya, Aichi 493-8001, Japan. kaihatsu@mri.biglobe.ne.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimoda
authors.forename: Junji
authors.initials: J
authors.lastname: Tanaka
authors.forename: Emi
authors.initials: E
authors.lastname: Yamada
authors.forename: Toshio
authors.initials: T
authors.lastname: Morikawa
authors.forename: Naoki
authors.initials: N
authors.lastname: Kasajima
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects*; D002460:Cell Line; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D017998:Leukotriene D4 / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000503:physiopathology; D036901:Petasites / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D006255:Rhinitis, Allergic, Seasonal / Q000503:physiopathology / Q000517:prevention & control*; D014132:Trachea / Q000503:physiopathology
chemical_list: D010936:Plant Extracts; D017998:Leukotriene D4; D006632:Histamine