title: Positive electrospray ion trap multistage mass spectrometric fragmentation of synthetic analogs of saccharide part of lipopolysaccharides of Vibrio cholerae O:1.
abstract: Oligosaccharides (mono- to hexamers) that mimic the terminal epitopes of O-antigens of Vibrio cholerae O:1, serotypes Ogawa and Inaba, have been studied by electrospray ion trap (ESI IT) mass spectrometry. Sodium or potassium-cationized adducts are characteristic ions under the conditions of ESI-MS analysis. The tentative pathways of fragmentation have been proven by multistage ion trap MS (MS(n), n = 1-3). The predominant pathway of fragmentation of the oligomers is the neutral loss of monosaccharide residue shortening the length of the oligosaccharide. In this way, conversion of the Ogawa to Inaba fragments takes place under the conditions of measurement. ESI MS/MS provided sufficient information about molecular mass, the number of saccharide residues, and the structure of saccharides, about the C (4)-amide of 3-deoxy-L-glycero-tetronic acid (DGT) of the compounds investigated, and allows to distinguish between Ogawa and Inaba serotypes.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravska cesta 9, 84538, Bratislava, Slovakia. chemvkov@savba.sk.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Kováčik
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravska cesta 9, 84538, Bratislava, Slovakia.
authors.forename: Slavka
authors.initials: S
authors.lastname: Bekešová
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravska cesta 9, 84538, Bratislava, Slovakia.
authors.forename: Igor
authors.initials: I
authors.lastname: Tvaroška
authors.affiliation: NIDDK, National Institute of Health, Bethesda, Maryland, USA.
authors.forename: Pavol
authors.initials: P
authors.lastname: Kováč
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry*; D002236:Carbohydrate Conformation; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014734:Vibrio cholerae / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D008070:Lipopolysaccharides