Culture conditions of Roseobacter strain 27-4 affect its attachment and biofilm formation as quantified by real-time PCR.
The fish probiotic bacterium Roseobacter strain 27-4 grows only as rosettes and produces its antibacterial compound under static growth conditions. It forms three-dimensional biofilms when precultured under static conditions. We quantified attachment of Roseobacter strain 27-4 using a direct real-time PCR method and demonstrated that the bacteria attached more efficiently to surfaces during static growth than under aerated conditions.
Bruhn
Jesper Bartholin
JB
Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, DTU Bldg. 221, DK-2800 Kongens Lyngby, Denmark. jbb@dfu.min.dk
Haagensen
Janus Anders Juul
JA
Bagge-Ravn
Dorthe
D
Gram
Lone
L
D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D011137:Polystyrenes; D041904:Roseobacter / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*
D003470:Culture Media; D004269:DNA, Bacterial; D011137:Polystyrenes