[Inhibition effect of fludarabine on the growth of myelodysplastic syndrome cell line MUTZ-1 and its mechanism].
To investigate the inhibition effect of fludarabine on the growth of human MDS-RAEB cell line MUTZ-1 and to explore the possible cellular and molecular mechanism.



The apoptosis of MUTZ-1 cells induced by fludarabine was studied by transmission electron microscope, MTT assay, DNA ladder test, flow cytometry and RT-PCR method.



Treatment with fludarabine remarkably inhibited the growth of MUTZ-1 cells, the 24 h IC(50), 48 h IC(50) and 72 h IC(50) of fludarabine for MUTZ-1 cells were 137.65 mg/L, 6.27 mg/L and 0.51 mg/L, respectively. Fludarabine inhibited the growth of MUTZ-1 cells in a dose-dependent and time-dependent manner. After treated by fludarabine (1 mg/L-16 mg/L)for 24 h, MUTZ-1 cells showed the typical features of apoptosis. After fludarabine treatment the mRNA expression of Bcl-2, Bax, survivin, XIAP, cIAP-1 and cIAP-2 was not changed, but the mitochondrial membrane potential (MMP) was decreased.



With a certain range of dose fludarabine (1 mg/L-16 mg/L)could inhibit MUTZ-1 cell growth by inducing cells apoptosis. MMP may play a certain role in apoptosis of MUTZ-1 cells induced by fludarabine.
Wu
Wen-jun
WJ
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Cai
Zhen
Z
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D000077022:Survivin; D014740:Vidarabine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics
D000970:Antineoplastic Agents; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D000077022:Survivin; D051028:bcl-2-Associated X Protein; D014740:Vidarabine; C024352:fludarabine