Human fibrinogen adsorption onto single-walled carbon nanotube films.
The adsorption behavior of human fibrinogen (Hfg) on single-walled carbon nanotube (SWNT) films was investigated using scanning electron microscopy (SEM) and near edge X-ray absorption fine structure (NEXAFS) spectroscopy. It was shown in the SEM images that fibrinogen was adsorbed strongly on the surface of SWNT when the samples were incubated in the Hfg solutions for 10 min. The dependence of adsorption on the concentration of fibrinogen was also investigated and it was found that adsorption increased with increasing concentration. In order to further explore the adsorption of fibrinogen on SWNT surface, NEXAFS spectra were obtained at the N K-edge and the C K-edge. The results confirmed the conclusion regarding the dependence of adsorption on fibrinogen concentration. It is demonstrated that, due to its high sensitivity to the surface elements, NEXAFS spectroscopy is a powerful tool to investigate the adsorption of fibrinogen on SWNT films.
Song
Li
L
Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080, China.
Meng
Jie
J
Zhong
Jun
J
Liu
Lifeng
L
Dou
Xinyuan
X
Liu
Dongfang
D
Zhao
Xiaowei
X
Luo
Sudong
S
Zhang
Zengxing
Z
Xiang
Yanjuan
Y
Xu
Haiyan
H
Zhou
Weiya
W
Wu
Ziyu
Z
Xie
Sishen
S
D000327:Adsorption; D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010316:Particle Size; D013057:Spectrum Analysis; D013499:Surface Properties; D014965:X-Rays
D008567:Membranes, Artificial; D037742:Nanotubes, Carbon; D005340:Fibrinogen