A BAC clone-based physical map of ovine major histocompatibility complex.
An ovine bacterial artificial chromosome (BAC) library containing 190,000 BAC clones was constructed and subsequently screened to construct a BAC-based physical map for the ovine major histocompatibility complex (MHC). Two hundred thirty-three BAC clones were selected by 84 overgo probes designed on human, mouse, and swine MHC sequence homologies. Ninety-four clones were ordered by DNA fingerprinting to form contigs I, II, and III that correspond to ovine MHC class I-class III, class IIa, and class IIb. The minimum tiling paths of contigs I, II, and III are 15, 4, and 4 BAC clones, spanning approximately 1900, 400, and 300 kb, respectively. The order and orientation of most BAC clones in each contig were confirmed by BAC-end sequencing. An open gap exists between class IIa and class III. This work helps to provide a foundation for detailed study of ovine MHC genes and of evolution of MHCs in mammals.
Liu
Haibo
H
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 5 Datun Road, Chaoyang District, Beijing 100101, China.
Liu
Ka
K
Wang
Jinfu
J
Ma
Runlin Z
RZ
D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial; D020451:Contig Mapping; D015723:Gene Library; D016678:Genome; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D017385:Sequence Homology; D012756:Sheep / Q000235:genetics*