Identification of disialic acid-containing glycoproteins in mouse serum: a novel modification of immunoglobulin light chains, vitronectin, and plasminogen.
Serum glycoproteins are involved in various biologic activities, such as the removal of exogenous antigens, fibrinolysis, and metal transport. Some of them are also useful markers of inflammation and disease. Although the amount of sialic acid increases following inflammation, little attention has been paid to the presence of linkage-specific epitopes in serum, especially the alpha2,8-linkage. In a previous study, we demonstrated that four components in mouse serum contain alpha2,8-linked disialic acid (diSia), based on immunoreactivity with monoclonal antibody 2-4B, which is specific to N-glycolylneuraminic acid (Neu5Gc)alpha2-->(8Neu5Gc alpha2-->)(n-1), n > or = 2 [Yasukawa et al., (2005) Glycobiology, 15, 827-837]. In this study, we purified three components, 30-, 70-, and 120-kDa gp, and identified them as an immunoglobulin (Ig) light chain, vitronectin, and plasminogen, respectively, using matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analyses. Modifications of these proteins with alpha2,8-linked diSia were chemically confirmed by fluorometric C7/C9 analyses and mild acid hydrolysates-fluorometric anion-exchange chromatography analyses. We also demonstrated that the IgG, IgM, and IgE light chains are commonly modified with alpha2,8-linked diSia. In addition, both mouse and rat vitronectin contained diSia, and the amount of disialylation in vitronectin dramatically decreased after hepatectomy. These results indicate that a novel diSia modification of serum glycoproteins is biologically important for immunologic events and fibrinolysis.
Yasukawa
Zenta
Z
Laboratory of Animal Cell Function, Bioscience and Biotechnology Center, Nagoya University, Japan.
Sato
Chihiro
C
Sano
Kotone
K
Ogawa
Haruko
H
Kitajima
Ken
K
D000595:Amino Acid Sequence; D000818:Animals; D019281:Dimerization; D006023:Glycoproteins / Q000097:blood / Q000737:chemistry* / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000097:blood / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010958:Plasminogen / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D051381:Rats; D012794:Sialic Acids / Q000032:analysis*; D019096:Vitronectin / Q000097:blood / Q000737:chemistry* / Q000378:metabolism
D006023:Glycoproteins; D007147:Immunoglobulin Light Chains; D012794:Sialic Acids; D019096:Vitronectin; D010958:Plasminogen