title: Pulmonary neuroendocrine cells, airway innervation, and smooth muscle are altered in Cftr null mice.
abstract: The amine- and peptide-producing pulmonary neuroendocrine cells (PNEC) are widely distributed within the airway mucosa of mammalian lung as solitary cells and innervated clusters, neuroepithelial bodies (NEB), which function as airway O2 sensors. These cells express Cftr and hence could play a role in the pathophysiology of cystic fibrosis (CF) lung disease. We performed confocal microscopy and morphometric analysis on lung sections from Cftr-/- (null), Cftr+/+, and Cftr+/- (control) mice at developmental stages E20, P5, P9, and P30 to determine the distribution, frequency, and innervation of PNEC/NEB, innervation and cell mass of airway smooth muscle, and neuromuscular junctions using synaptic vesicle protein 2, smooth muscle actin, and synaptophysin markers, respectively. The mean number of PNEC/NEB in Cftr-/- mice was significantly reduced compared with control mice at E20, whereas comparable or increased numbers were observed postnatally. NEB cells in Cftr null mice showed a significant reduction in intracorpuscular nerve endings compared with control mice, which is consistent with an intrinsic abnormality of the PNEC system. The airways of Cftr-/- mice showed reduced density (approximately 20-30%) of smooth muscle innervation, decreased mean airway smooth muscle mass (approximately 35%), and reduced density (approximately 20%) of nerve endings compared with control mice. We conclude that the airways of Cftr-/- mice exhibit heretofore unappreciated structural alterations affecting cellular and neural components of the PNEC system and airway smooth muscle and its innervation resulting in blunted O2 sensing and reduced airway tonus. Cftr could play a role in the development of the PNEC system, lung innervation, and airway smooth muscle.
authors.affiliation: Division of Pathology, Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: Jie
authors.initials: J
authors.lastname: Pan
authors.forename: Catherine
authors.initials: C
authors.lastname: Luk
authors.forename: Geraldine
authors.initials: G
authors.lastname: Kent
authors.forename: Ernest
authors.initials: E
authors.lastname: Cutz
authors.forename: Herman
authors.initials: H
authors.lastname: Yeger
mesh_terms: D000818:Animals; D002452:Cell Count; D003550:Cystic Fibrosis / Q000235:genetics / Q000473:pathology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000502:physiology*; D008168:Lung / Q000254:growth & development / Q000294:innervation* / Q000473:pathology; D051379:Mice; D018181:Mice, Inbred CFTR; D009130:Muscle, Smooth / Q000294:innervation / Q000473:pathology*; D009411:Nerve Endings / Q000473:pathology; D046568:Neuroepithelial Bodies / Q000473:pathology*; D009469:Neuromuscular Junction / Q000473:pathology; D009489:Neurosecretion / Q000235:genetics
chemical_list: D019005:Cystic Fibrosis Transmembrane Conductance Regulator