title: Chiral separation of cefadroxil by capillary electrochromatography.
abstract: In this paper, the chiral separation of cefadroxil was studied by capillary electrochromatography. Monolithic capillary column was prepared for the separation of cefadroxil enantiomers. The optimum buffer contained 28.5 mmol/L sodium acetate, 0.95% (v/v) acetic acid, 19 mmol/L beta-cyclodextrin (beta-CD) and 5% (v/v) isopropanol in formamide solution (pH 7.0), with the running voltage of 12 kV, the UV detector wavelength of 254 nm, the sample injected time of 8s and the temperature of 25 degrees C. Under these conditions, the column efficiency of cefadroxil enantiomers were N1=5324 and N2=23,768 with a selectivity factor (alpha) of 1.056 and resolution (Rs) of 0.978. The effect of buffer pH value, beta-CD concentration, organic modifier (isopropanol) concentration and voltage was also investigated for the separation by CEC.
authors.affiliation: Department of Chemistry, Weifang University, Weifang 261061, China. haixingliu@tom.com
authors.forename: Haixing
authors.initials: H
authors.lastname: Liu
authors.forename: Aimin
authors.initials: A
authors.lastname: Yu
authors.forename: Fengqin
authors.initials: F
authors.lastname: Liu
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Shi
authors.forename: Likun
authors.initials: L
authors.lastname: Han
authors.forename: Yunfa
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002434:Cefadroxil / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods; D013237:Stereoisomerism
chemical_list: D000900:Anti-Bacterial Agents; D002434:Cefadroxil