title: Rickettsial agents from parasitic dermanyssoidea (Acari: Mesostigmata).
abstract: Mites are often overlooked as vectors of pathogens, but have been shown to harbor and transmit rickettsial agents such as Rickettsia akari and Orientia tsutsugamushi. We screened DNA extracts from 27 mites representing 25 species of dermanyssoids for rickettsial agents such as Anaplasma, Bartonella, Rickettsia, and Wolbachia by PCR amplification and sequencing. DNA from Anaplasma spp., a novel Bartonella sp., Spiroplasma sp., Wolbachia sp., and an unclassified Rickettsiales were detected in mites. These could represent mite-borne bacterial agents, bacterial DNA from blood meals, or novel endosymbionts of mites.
authors.affiliation: Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, MS G-13, Atlanta, GA 30333, USA. cui8@cdc.gov
authors.forename: Will K
authors.initials: WK
authors.lastname: Reeves
authors.forename: Ashley P G
authors.initials: AP
authors.lastname: Dowling
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Dasch
mesh_terms: D000709:Anaplasma / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D001473:Bartonella / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification*; D008925:Mites / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013149:Spiroplasma / Q000235:genetics / Q000302:isolation & purification; D020577:Wolbachia / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S