title: Expression profiling of auxin-treated Arabidopsis roots: toward a molecular analysis of lateral root emergence.
abstract: Treating Arabidopsis roots with exogenous auxin results in dramatic changes in cellular processes including de novo induction of lateral roots which later emerge through the overlying cells. Microarray experiments reveal approximately 80 genes that are substantially up-regulated in the root over the first 12 h following auxin treatment. We hypothesize that the observed increase in expression of pectate lyase family genes leads to degradation of the pectin-rich middle lamellae, allowing cells in the parent root to separate cleanly. Differences in the degree of pectin methylation in lateral and parent roots may explain why lateral roots are not degraded themselves.
authors.affiliation: Biology Department, Oberlin College, Oberlin, OH 44074, USA. mlaskows@oberlin.edu
authors.forename: Marta
authors.initials: M
authors.lastname: Laskowski
authors.forename: Steven
authors.initials: S
authors.lastname: Biller
authors.forename: Ken
authors.initials: K
authors.lastname: Stanley
authors.forename: Tymoteusz
authors.initials: T
authors.lastname: Kajstura
authors.forename: Reeta
authors.initials: R
authors.lastname: Prusty
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D002473:Cell Wall / Q000187:drug effects / Q000502:physiology; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008745:Methylation; D020411:Oligonucleotide Array Sequence Analysis; D010741:Phospholipases A / Q000032:analysis / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*; D011133:Polysaccharide-Lyases / Q000032:analysis / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D007210:Indoleacetic Acids; D010741:Phospholipases A; D011133:Polysaccharide-Lyases; C468914:AT1G67750 protein, Arabidopsis; C513206:PLA2 protein, Arabidopsis; C023911:pectate lyase