MITF and SgIGSF: an essential transcription factor and its target adhesion molecule for development and survival of mast cells.
MITF transcription factor is not produced in mutant mice of WBB6F1-Mitf(mi-vga9/Mitf(mi-vga9). When bone marrow cells of normal WBB6F1-+/+ micewere transplanted to the irradiated WBB6F1-KitW/KitW-v mice, which are genetically mast cell deficient, erythrocytes, neutrophils, macrophages, B cells and T cells of the donor origin developed in the recipients. On the other hand, when bone marrow cells of WBB6F1-Mitf(mi-vga9/Mitf(mi-vga9) mice were transplanted to similarly irradiated WBB6F1-KitW/KitW-v mice, erythrocytes, neutrophils, macrophages, B cells and T cells of the donor origin developed but mast cells never appeared. To identify the targets of MITF, we elaborated a subtracted cDNA library between cultured mast cells (CMCs) derived from +/+ mice and CMCs from MITF mutant mice. We obtained a clone encoding SgIGSF, which was expressed on the adhesion surface of +/+ CMCs attaching to fibroblasts. CMCs of WBB6F1-Mitf(mi-vga9/ Mitf(mi-vga9) mice did not express SgIGSF, did not attach to fibroblasts and did not survive in the peritoneal cavity of WBB6F1-KitW/KitW-v mice. When MITF or SgIGSF cDNA was transfected to WBB6F1-Mitf(mi-vga9/Mitf(mi-vga9) CMCs, they attached to fibroblasts and showed an improved survival in the peritoneal cavity of WBB6F1-KitW/KitW-v mice. MITF and SgIGSF appeared essential for the development and survival of mast cells in tissues of adult WBB6F1-KitW/KitW-v mice.
Kitamura
Yukihiko
Y
Developmental Research Laboratories, Shionogi & Co., Futaba-cho 3-1-1, Toyonaka, Osaka 561-0825, Japan.
D000818:Animals; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002470:Cell Survival; D017690:Cell Transplantation; D002633:Chemotaxis / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D050980:GATA Transcription Factors / Q000235:genetics / Q000378:metabolism; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D008407:Mast Cells / Q000166:cytology / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism
C498598:Cadm1 protein, mouse; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D050980:GATA Transcription Factors; D007136:Immunoglobulins; D008565:Membrane Proteins; D051739:Microphthalmia-Associated Transcription Factor; C497235:Mitf protein, mouse; D020033:Protein Isoforms; D019009:Proto-Oncogene Proteins c-kit