[High-level expression of phenylalanine ammonia-lyase in Lactococcus Lactis via synthesized sequence based on bias codons].
To construct a safer and more efficient gene engineering Lactococcus Lactis for expressing phenylalaine ammonia lyase (PAL) which will be benefit for PKU therapy, pal cDNA of Parsly and synthesized sequence based on Lactococcus Lactis bias codons were recombined into two Lactococcus Lactis NICE systems. The activities of the expressed PAL were detected, and the effect of Lactococcus Lactis bias codons on the expression of exterior protein was analyzed. The results showed that the expression level of PAL was increased by using Lactococcus Lactis bias codons in both Lactococcus Lactis NICE systems. Through which several safer andmore efficient strains of the gene engineering Lactococcus Lactis were obtained.
Chen
Xing
X
Beijing Chaoyang Hospital Affiliate of Capital University of Medical Sciences, Beijing 100020, China.
Gao
Bin
B
Jia
Xing-Yuan
XY
Su
Chang
C
Lü
Yue-Ping
YP
Wang
Zhan-Yong
ZY
Fan
Xin-Ping
XP
Xiao
Bai
B
Liu
Jing-Zhong
JZ
D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000378:metabolism; D014169:Transformation, Bacterial
D003062:Codon; D011994:Recombinant Proteins; D010650:Phenylalanine Ammonia-Lyase