title: Comment on "PDK1 nucleates T cell receptor-induced signaling complex for NF-kappaB activation".
abstract: We observe that protein kinase C (PKC) is phosphorylated on the activation loop at threonine 538 (Thr-538) before T cell activation. Our results are inconsistent with the conclusions of Lee et al. (Reports, 1 April 2005, p. 114) that the Thr-538 phosphorylation of PKC is regulated by T cell receptor activation. Other mechanisms, such as autophosphorylation of Thr-219, might orchestrate the cellular function of PKC in T cells.
authors.affiliation: Department for Medical Genetics, Molecular and Clinical Pharmacology, Innsbruck Medical University, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Gruber
authors.forename: Michael
authors.initials: M
authors.lastname: Freeley
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Thuille
authors.forename: Isabelle
authors.initials: I
authors.lastname: Heit
authors.forename: Stephen
authors.initials: S
authors.lastname: Shaw
authors.forename: Aideen
authors.initials: A
authors.lastname: Long
authors.forename: Gottfried
authors.initials: G
authors.lastname: Baier
mesh_terms: D064413:3-Phosphoinositide-Dependent Protein Kinases; D000818:Animals; D000906:Antibodies / Q000276:immunology; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D000074121:Protein Kinase C-theta; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism
chemical_list: D000906:Antibodies; D018106:CD28 Antigens; D017252:CD3 Complex; D007527:Isoenzymes; D016328:NF-kappa B; D011948:Receptors, Antigen, T-Cell; D013912:Threonine; D064413:3-Phosphoinositide-Dependent Protein Kinases; C578562:PDPK1 protein, human; C578564:Pdpk1 protein, mouse; D017346:Protein Serine-Threonine Kinases; C076233:PRKCQ protein, human; C489593:Prkcq protein, mouse; D011493:Protein Kinase C; D000074121:Protein Kinase C-theta