title: Patients with gastrointestinal complains due to enteric parasites, with reference to Entamoeba histolytica/dispar as dected by ELISA E. histolytica adhesion in stool.
abstract: A total of 210 patients with gastrointestinal troubles, of both sex and a mean age of 32 +/- 6.1 years, selected from the outpatient's clinics of Al-Azhar University Hospitals. 115 (54.76%) had dysentery, 95 (45.23%) did not have dysentery, 15 (14%) suffered flatulence, 20 (9.52%) had epi-gastric pain, 19 (9.05%) had vague abdominal pain, 5 vomiting (5.2%) and 10 (4.9%) had fever. Two symptoms were in 29 (13.81%) patients and three symptoms in 12 (5.71%). Of the 210 patients, 20 (9.9%) had helminthes infection, 121 (57.6%) had intestinal protozoa and 69 (32.9%) had no parasitic infection. Of these parasite-free patients, 16 had Shigella sp. and nine had Campylobacter sp. Of the patients with intestinal protozoa, 34 (16.2%) had E. histolytica/dispar by stool examination of stained smears. By using ELISA for detection of E. histolytica adhesion in stool samples of 115 with diarrhea only 18 had true E. histolytica infection and of 3 without diarrhea only one had E. histolytica infection. Mean-while, ELISA did not cross-reacted E. coli, Giardia lamblia, Cryptosporidium parvum, Endolimax nana or Blastocystis hominis. So, ELISA for detection of E. histolytica adhesion in stool samples was more specific than microscopy and safe direction to the E. histolytica treatment. Apart from intestinal protozoan and bacteria, helminthes were seen in stool analysis. These were Schistosoma mansoni (0.95%), Capillaria sp. (0.95%), Enterobius vermicularis (1.90%) macroscopically, Hymenolepis nana (4.3%) and Ascaris lumbricoides (1.43%).
authors.affiliation: Department of Parasitology, Faculty of Medicine, Al Azhar University, Cairo, Egypt.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: El-Kadi
authors.forename: Ahmad O
authors.initials: AO
authors.lastname: Dorrah
authors.forename: Nahla M
authors.initials: NM
authors.lastname: Shoukry
mesh_terms: D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D003429:Cross Reactions; D003937:Diagnosis, Differential; D003967:Diarrhea / Q000382:microbiology / Q000469:parasitology*; D004404:Dysentery, Amebic / Q000175:diagnosis* / Q000453:epidemiology; D004748:Entamoeba histolytica / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005243:Feces / Q000382:microbiology / Q000469:parasitology*; D005260:Female; D005414:Flatulence / Q000209:etiology; D006373:Helminthiasis / Q000175:diagnosis / Q000453:epidemiology; D006801:Humans; D008297:Male; D010146:Pain / Q000209:etiology; D012680:Sensitivity and Specificity
chemical_list: D000913:Antibodies, Protozoan