Regulation of human neurotropic JCV in colon cancer cells.
BACKGROUND
Recently, the genome of the human polyomavirus, JC (JCV), and expression of its early and late regulatory proteins (T-antigen and agnoprotein) have been demonstrated in neoplastic cells of colonic cancer cases.


METHODS
Regulation of JCV was investigated in a human colon cancer cell line (SW480) and compared to a human glioblastoma cell line (U87-MG) that is permissive for JCV replication.


RESULTS
SW480 cells supported basal transcription of both early and late JCV promoters. The expression of TCF-4, a component of Wnt signaling, modulated JCV transcription in a cell type-specific manner. Both TCF-4 and T-antigen bound to the JCV promoter region and bound to each other. In addition, the expression of TCF-4 caused a decrease in the ability of the T-antigen to stimulate viral DNA replication in U87-MG cells.


CONCLUSIONS
Wnt pathway signaling proteins and T-antigen interact to regulate JCV in colonic epithelial cells.
Enam
Sahnila
S
Center for Neurovirology, Department of Neuroscience, Temple University School of Medicine, Philadelphia, PA 19122, USA.
Gan
Dai-Di
DD
White
Martyn K
MK
Del Valle
Luis
L
Khalili
Kamel
K
D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D005909:Glioblastoma / Q000235:genetics / Q000821:virology; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D011401:Promoter Regions, Genetic; D015398:Signal Transduction; D051820:TCF Transcription Factors / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic; D014162:Transfection; D014779:Virus Replication; D051153:Wnt Proteins / Q000235:genetics / Q000502:physiology
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D051820:TCF Transcription Factors; D051153:Wnt Proteins