Rapid detection and identification of Mycobacterium tuberculosis by Real Time PCR and Bactec 960 MIGT.
We have developed a Real-Time PCR assay to detect M. tuberculosis using the iCycler iQ detection system by TaqMan assay directly on the clinical specimen. A total of 513 clinical samples were taken from patients with suspected tuberculosis and other patients that had an active mycobacterial infection, as well as patients with diagnosed tuberculosis who were receiving antitubercular therapy. The sensitivity and specificity of this assay, 10% and 100%, respectively, were compared to those of conventional microbiological methods.
Ortu
Silvia
S
Dip. di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli Studi di Sassari, Italy. silviaortu@vahoo.it
Molicotti
Paola
P
Sechi
Leonardo Antonio
LA
Pirina
Pierp
P
Saba
Franca
F
Vertuccio
Cono
C
Deriu
Antonella
A
Maida
Ivana
I
Mura
Maria Stella
MS
Zanetti
Stefania
S
D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000737:chemistry; D014376:Tuberculosis / Q000382:microbiology*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D019914:Taq Polymerase