[Protective effect of mouse 2.5s nerve growth factor on PC12 cells from injury induced by 2, 5-hexanedione].
OBJECTIVE
To explore whether the nerve growth factor has protective effects on PC12 cells from injury induced by 2, 5-hexanedione.


METHODS
With PC12 cells as the model of neurons, different concentrations of NGF were added into the culture of PC12 cells. Then cell viability was tested with MTT. The DNA fragment was observed with agarose gel electrophoresis. The apoptosis ratio was tested with flow cytometry (FACS). The p53 protein was detected with western blot. The differences among the groups were compared.


RESULTS
Cell viabilities were increased with the increase of the concentrations of NGF (P < 0.05). The DNA fragment, the apoptosis ratio and the expression of p53 were all decreased with the increase of the concentrations of NGF (P < 0.05).


CONCLUSIONS
The NGF might have direct nutritional effects on PC12 cells, and protect them from injury induced by 2, 5 HD. Moreover, it might also have anti-apoptosis effect to some extent.
Sun
Ling-cong
LC
Department of Biochemistry and Molecular Biology, School of Medicine, Wuhan University, Wuhan 430071, China.
Xia
La-ju
LJ
Meng
Xiang-ping
XP
Liu
Li
L
Gao
Xing-hua
XH
Yang
Guo-cheng
GC
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D006588:Hexanones / Q000633:toxicity*; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
D006588:Hexanones; D009414:Nerve Growth Factors; D016159:Tumor Suppressor Protein p53; C011269:2,5-hexanedione