Direct determination of p-hydroxymethamphetamine glucuronide in human urine by high-performance liquid chromatography.
High-performance liquid chromatography (HPLC) with UV detection for the simultaneous determination of the free form of p-hydroxymethamphetamine (p-OHMA) and its metabolite, glucuronide (p-OHMAG) was accomplished for the first time. We achieved this by employing 1) an ion pair reagent for retention of sample to a solid-phase extraction (SPE) cartridge, Sep-Pak Light C18 and 2) a simple two-step stepwise elution technique for subsequent ion pair RP-HPLC. The proposed method was optimized for resolution of p-OHMAG, p-OHMA and MA. The method was successfully applied to urine samples collected from MA abusers.
Nakajima
Rika
R
School of Medicine, Keio University, Tokyo, Japan. nakaji@sc.itc.keio.ac.jp
Morishita
Muneo
M
Aiso
Sadakazu
S
Yajima
Takehiko
T
D019969:Amphetamine-Related Disorders / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D020719:Glucuronides / Q000652:urine*; D006801:Humans; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000652:urine; D013237:Stereoisomerism
D020719:Glucuronides; D008694:Methamphetamine; C005877:pholedrine