The effect of sulfide on alpha-glucosidases: implications for starch degradation in anaerobic bioreactors.
Membrane associated alpha-glucosidase activity was investigated in a methanogenic bioreactor (MR) and a biosulfidogenic bioreactor (SR). Temperature and pH optima studies showed temperature optima of 50 degrees C and pH optima of 8.0 for the alpha-glucosidases from both the MR and SR. Sulfide (at a concentration of 150 mg l(-1)) resulted in the complete loss of all alpha-glucosidase activity in both the MR and SR. beta-Glucosidase activities in our bioreactors were previously shown to be stimulated in the presence of sulfide. alpha-Glucosidases, in contrast, are inhibited by sulfide. This differential effect of sulfide on alpha-glucosidase and beta-glucosidase activities is highlighted and is of crucial consequence to the respective degradation and utilization of starch and cellulose substrates in natural anaerobic environments and anaerobic bioreactors specifically designed for the accelerated digestion of wastewater sludge under biosulfidogenic conditions.
Watson
S D
SD
Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, Grahamstown 6140, South Africa.
Pletschke
B I
BI
D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D017018:Methanomicrobiales / Q000201:enzymology / Q000254:growth & development; D013213:Starch / Q000032:analysis*; D013440:Sulfides / Q000737:chemistry*; D016999:Sulfur-Reducing Bacteria / Q000201:enzymology / Q000254:growth & development; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification / Q000379:methods*; D000520:alpha-Glucosidases / Q000378:metabolism*
D013440:Sulfides; D014874:Water Pollutants, Chemical; D013213:Starch; D000520:alpha-Glucosidases