Comparison of two automated immunohistochemical procedures for the diagnosis of scrapie in domestic sheep and chronic wasting disease in North American white-tailed deer (Odocoileus virginianus) and mule deer (Odocoileus hemionus).
Two commercially available automated immunohistochemistry platforms, Ventana NexES and DakoCytomation Autostainer Universal Staining System, were compared for diagnosing sheep scrapie and cervid chronic wasting disease. Both automated platforms used the same antiprion protein monoclonal primary antibodies, but different platform-specific linker and amplification reagents and procedures. Duplicate sections of brainstem (at the level of the obex) and lymphoid tissue (retropharyngeal lymph node or tonsil) from the same tissue block were immunostained for the comparison. Examination of 1,020 tissues from 796 sheep revealed 100% concordance of results between the Ventana NexES and DakoCytomation platforms for diagnosing sheep scrapie from lymphoid tissue (103/103 positive; 405/405 negative) and brainstem (120/120 positive; 392/392 negative). Similarly, examination of 1,008 tissues from 504 white-tailed deer revealed 100% concordance between the Ventana NexES and DakoCytomation platforms for diagnosing chronic wasting disease from lymphoid tissue (104/104 positive; 400/400 negative) and brainstem (104/104 positive; 400/400 negative). Examination of 1,152 tissues from 482 mule deer revealed a concordance of 98.6% in lymphoid tissue and 99.9% in brainstem between the Ventana NexES and DakoCytomation platforms for diagnosing chronic wasting disease. The results indicate equivalence or near equivalence between the DakoCytomation and Ventana NexES autostainer platforms for identification of the disease-associated prion protein (PrPd)-positive and PrPd-negative brain and lymphoid tissues in sheep, white-tailed deer, and mule deer.
Baszler
T V
TV
Washington Animal Disease Diagnostic Laboratory and Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman 99165-20371, USA. baszlert@vetmed.wsu.edu
Kiupel
M
M
Williams
E S
ES
Thomsen
B V
BV
Gidlewski
T
T
Montgomery
D L
DL
O'Rourke
K I
KI
Hall
S M
SM
D000818:Animals; D000911:Antibodies, Monoclonal; D001933:Brain Stem / Q000378:metabolism / Q000473:pathology; D004396:Coloring Agents; D003670:Deer; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D011328:Prions / Q000378:metabolism; D012608:Scrapie / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D012756:Sheep; D034081:Wasting Disease, Chronic / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology
D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D011328:Prions