Efficient differentiation into skin cells of bone marrow cells recovered in a pellet after density gradient fractionation.
We had previously demonstrated the participation of whole bone marrow cells from adult mice in the reconstitution of skin, including the epidermis and hair follicles. To get an insight into cell populations that give rise to the epithelial components of the reconstituted skin, we fractionated bone marrow cells derived from green fluorescent protein-transgenic mice by density gradient. Unexpectedly, we found that a substantial amount of mononucleated cells (approximately 30%) was recovered in the pellet fraction and that the cells in the pellet fraction preferentially differentiated into epithelial components of skin, rather than the cells in the mononuclear cell fraction. The pellet fraction contained more CD45-negative (thus uncommitted to the hematopoietic cell lineage) cells than the mononuclear cell fraction. These results indicate that density gradient fractionation results in significant loss of specific progenitor cells into the usually discarded pellet fraction.
Medina
Reinhold J
RJ
Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700-8558, Japan.
Kataoka
Ken
K
Miyazaki
Masahiro
M
Huh
Nam-Ho
NH
D000818:Animals; D001854:Bone Marrow Cells / Q000737:chemistry / Q000166:cytology*; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018859:Hair Follicle / Q000737:chemistry / Q000166:cytology; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000032:analysis; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology; D017493:Leukocyte Common Antigens / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D008819:Mice, Nude; D008822:Mice, Transgenic; D012627:Sebaceous Glands / Q000737:chemistry / Q000166:cytology; D012867:Skin / Q000737:chemistry / Q000166:cytology*; D016038:Skin Transplantation / Q000379:methods
D019012:Integrin beta1; D049452:Green Fluorescent Proteins; D017493:Leukocyte Common Antigens