Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells.
We investigated the intracellular uptake of different sized and shaped colloidal gold nanoparticles. We showed that kinetics and saturation concentrations are highly dependent upon the physical dimensions of the nanoparticles (e.g., uptake half-life of 14, 50, and 74 nm nanoparticles is 2.10, 1.90, and 2.24 h, respectively). The findings from this study will have implications in the chemical design of nanostructures for biomedical applications (e.g., tuning intracellular delivery rates and amounts by nanoscale dimensions and engineering complex, multifunctional nanostructures for imaging and therapeutics).
Chithrani
B Devika
BD
Institute of Biomaterials & Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada.
Ghazani
Arezou A
AA
Chan
Warren C W
WC
D006046:Gold / Q000737:chemistry* / Q000493:pharmacokinetics*; D006367:HeLa Cells; D006801:Humans; D008657:Metabolic Clearance Rate; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size
D006046:Gold