title: Electroantennogram and behavioral responses of Culex quinquefasciatus (Diptera: Culicidae) females to chemicals found in human skin emanations.
abstract: The southern house mosquito, Culex quinquefasciatus Say (Diptera: Culicidae), is closely associated with humans and is a vector of filariasis. Use of semiochemicals for control of mosquitoes is a novel and potentially ecofriendly control approach. Human skin emanations are known to attract mosquitoes. In the current study, olfactory and behavioral responses of host-seeking female Cx. quinquefasciatus to various chemical components of human skin emanations (carboxylic acids, alcohols, and aldehydes) were evaluated separately at three doses (0.01, 0.1, and 1 microg) for electroantennogram (EAG) and three doses (0.1, 1, and 10 microg) for behavioral assay. Results of EAG studies indicated that all carboxylic acids elicited significant olfactory responses except tetradecanoic acid (C14) and octadecanoic acid (C18). In particular, hexanoic acid (C6) elicited a maximum, eight-fold olfactory response compared with the solvent control. Ethylene glycol and benzyl alcohol exhibited significant EAG and behavioral responses, whereas among aldehydes, nonanal elicited high EAG and behavioral responses, but only at all three doses tested compared with control. Some carboxylic acids elicited significant behavioral responses, attracting approximately 75% of females tested toward chemical stimuli in a Y-tube olfactometer.
authors.affiliation: Defense Research and Development Establishment, Jhansi Road, Gwalior 474002, India.
authors.forename: Sarika N
authors.initials: SN
authors.lastname: Puri
authors.forename: M J
authors.initials: MJ
authors.lastname: Mendki
authors.forename: D
authors.initials: D
authors.lastname: Sukumaran
authors.forename: K
authors.initials: K
authors.lastname: Ganesan
authors.forename: Shri
authors.initials: S
authors.lastname: Prakash
authors.forename: K
authors.initials: K
authors.lastname: Sekhar
mesh_terms: D000438:Alcohols / Q000494:pharmacology*; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D002264:Carboxylic Acids / Q000494:pharmacology*; D003465:Culex / Q000187:drug effects / Q000502:physiology*; D004563:Electrochemistry; D005260:Female; D005426:Flight, Animal / Q000502:physiology; D006801:Humans; D007303:Insect Vectors / Q000187:drug effects / Q000502:physiology*; D012867:Skin / Q000737:chemistry; D014919:Wind
chemical_list: D000438:Alcohols; D000447:Aldehydes; D002264:Carboxylic Acids