Influence of pertussis toxin on CD1a isoform expression in human dendritic cells.
Pertussis toxin (PTX) is an exotoxin produced by Bordetella pertussis. It is known to exert adjuvant activities inducing Th1-launched immune responses. In this study, we show that PTX can selectively block the expression of CD1a isoform during the differentiation of human monocytes into dendritic cells. In fact, dendritic cells differentiated from monocytes in the presence of PTX do not express CD1a on their surface, unlike CD1b and CD1c isoforms, which are normally regulated. The impaired CD1a expression on cell membrane depends, at least partially, on decreased mRNA transcription and does not affect cellular capability to respond to other maturation stimuli. Since CD1a(+) dendritic cells are involved in the early steps of primary immune response, the interference of PTX in the CD1a expression may be relevant for its employment as adjuvant.
Martino
Angelo
A
National Institute for Infectious Diseases, "Lazzaro Spallanzani," IRCCS, Rome, Italy. martino@inmi.it
Volpe
Elisabetta
E
Auricchio
Giovanni
G
Colizzi
Vittorio
V
Baldini
Patrizia Morena
PM
D018949:Antigens, CD1 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods; D015870:Gene Expression; D006801:Humans; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D037342:Pertussis Toxin / Q000276:immunology / Q000494:pharmacology*; D018417:Th1 Cells / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000276:immunology
D018949:Antigens, CD1; C419393:CD1a antigen; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D037342:Pertussis Toxin