Role of secondary attachment sites in changing the specificity of site-specific recombination.
We previously proposed that lambdoid phages change their insertion specificity by adapting their integrases to sequences found in secondary attachment sites. To test this model, we quantified recombination between partners that carried sequences from secondary attachment sites catalyzed by wild-type and by mutant integrases with altered specificities. The results are consistent with the model, and indicate differential core site usage in excision and integration.
Rutkai
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E
Department of Molecular Biotechnology, Bay Zoltán Institute for Biotechnology, Szeged, Hungary.
György
Andrea
A
Dorgai
László
L
Weisberg
Robert A
RA
D001287:Attachment Sites, Microbiological / Q000235:genetics / Q000502:physiology*; D010582:Bacteriophage lambda / Q000201:enzymology / Q000502:physiology*; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D011995:Recombination, Genetic; D016662:Virus Integration
D019426:Integrases