Protein free diet feeding: effects on sympathetic activity and salivary evoked secretion in the submandibular gland of the rat.
Protein restriction impairs the salivary flow rate and composition in human and rats. The aim of the present work was to establish the effect of low protein (casein 5%) and protein free (casein 0%) isocaloric diets on sympathetic activity and salivary evoked secretion in the submandibular gland (SMG) of the rat. After 21 days, rats fed casein 0% presented: (a) a significant shift to the left of the dose-response curves (DRC) to the autonomic agonists-norepinephrine (NE), methoxamine, isoproterenol (ISO) and methacholine; (b) increased food consumption (p<0.001); (c) decreased body (p<0.001) and SMG (p<0.001) weights maintaining SMG/body (w/w) relation; (d) enhanced submandibular alpha1-adrenoceptor number without changes in the apparent dissociation constant (Kd); (e) increased submandibular NE content (p<0.05) and phosphoinositoside hydrolysis (p<0.001); (f) decreased submandibular tyrosine hydroxylase activity (TH) (p<0.01). Casein 5% feeding increased food consumption (p<0.01) and reduced body weight (p<0.05). This protein restriction increased metacholine-evoked salivation, but it altered neither submandibular sympathetic activity nor sympathetic-induced salivary secretion as compared to the Control group (C) fed a similar diet containing 25.5% protein. Present results suggest that in the adult rat, a protein free diet during 21 days lowers SMG sympathetic and cholinergic activity leading to supersensitivity as revealed by up-regulation of alpha1-adrenergic receptor number and increased autonomic-evoked salivation.
Elverdin
Juan C
JC
Cátedra de Fisiología, Facultad de Odontología, Universidad de Buenos Aires, M.T. de Alvear 2142, 3(er) Piso A (CP1122), Buenos Aires, Argentina. jelverdin@hotmail.com
Chiarenza
A P
AP
Luchelli
M A
MA
Vatta
M
M
Bianciotti
L G
LG
Boyer
P
P
Vacas
M I
MI
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002364:Caseins / Q000008:administration & dosage* / Q000378:metabolism; D018679:Cholinergic Agonists / Q000494:pharmacology; D018753:Diet, Protein-Restricted; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D008729:Methoxamine / Q000494:pharmacology; D023421:Models, Animal; D010716:Phosphatidylinositols / Q000378:metabolism; D011502:Protein-Energy Malnutrition / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012472:Salivation / Q000187:drug effects*; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis / Q000378:metabolism
D000316:Adrenergic alpha-Agonists; D000318:Adrenergic beta-Agonists; D015415:Biomarkers; D002364:Caseins; D018679:Cholinergic Agonists; D010716:Phosphatidylinositols; D016210:Methacholine Chloride; D014446:Tyrosine 3-Monooxygenase; D008729:Methoxamine; D007545:Isoproterenol