[Association between vitamin D receptor gene polymorphism and vitamin D deficiency rickets].
OBJECTIVE
To explore the genetic susceptibility of children to vitamin D deficiency rickets through studying the association between Vitamin D receptor (VDR) gene polymorphism and vitamin D deficiency rickets.


METHODS
One hundred and fifty-nine children (100 boys and 59 girls, aged 0 to 2 years), with new-onset vitamin D deficiency rickets were enrolled. The patients sampled from a community of Jiamusi City, Heilongjiang Province. Seventy-eight healthy age-matched children (46 boys and 32 girls) were used as the controls. VDR gene polymorphism (cleaved by restriction endonuclease Fok I) was analyzed by polymerase chase reaction-restriction fragment length polymorphism (PCR-RFLP). The frequencies of the VDR genotype and allele were compared between the two groups.


RESULTS
The frequencies of FF, Ff and ff genotypes were 37%, 51% and 12% in the Rickets group, and 18%, 55% and 27% in the Control group. A significant difference was found in the frequency distribution of the VDR genotype between the two groups (chi(2)(0.01(2))=9.210, chi(2)=13.3880, P < 0.01). In the Rickets group, f allele frequency was lower (37% vs 54%), while the F allele was more common than the Control group (63% vs 46%).


CONCLUSIONS
There is an association between the VDR gene Fok I polymorphism and vitamin D deficiency rickets. The individuals with the F allele are more susceptible to vitamin D deficiency rickets.
Wu
Sheng-Hu
SH
Department of Child Health Care, Shanghai Institute for Pediatric Research, Xinhua Hospital, Second Medical University, Shanghai 200092, China. wshenghu@hotmail.com
Yu
Xiao-Dan
XD
Yan
Chong-Huai
CH
Shen
Li-Xiao
LX
Yu
Xiao-Gang
XG
Zhang
Yan-Ping
YP
Zhang
Jin-Song
JS
Jin
Xing-Ming
XM
Shen
Xiao-Ming
XM
D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011110:Polymorphism, Genetic; D018167:Receptors, Calcitriol / Q000235:genetics*; D012279:Rickets / Q000235:genetics*
D018167:Receptors, Calcitriol