title: Misexpression screen for genes altering the olfactory map in Drosophila.
abstract: Despite the identification of a number of guidance molecules, a comprehensive picture has yet to emerge to explain the precise anatomy of the olfactory map. From a misexpression screen of 1,515 P{GS} lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of the Drosophila antennal lobes. These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling. We analyzed Akap200, which encodes a Protein Kinase A-binding protein. Overexpression of Akap200 resulted in fusion of the glomeruli, while its loss resulted in misshapen and ectopic glomeruli. The requirement of Akap200 validates our screen as an effective approach for recovering genes controlling glomerular map patterning. Our finding of diverse classes of genes reveals the complexity of the mechanisms that underlie olfactory map development.
authors.affiliation: Cell and Developmental Biology, University of Illinois at Urbana-Champaign, 601 South Goodwin Avenue, Urbana, IL 61801, USA.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhang
authors.forename: Weiguang
authors.initials: W
authors.lastname: Zhou
authors.forename: Chong
authors.initials: C
authors.lastname: Yin
authors.forename: Weitao
authors.initials: W
authors.lastname: Chen
authors.forename: Rie
authors.initials: R
authors.lastname: Ozawa
authors.forename: Lay-Hong
authors.initials: LH
authors.lastname: Ang
authors.forename: Lavanya
authors.initials: L
authors.lastname: Anandan
authors.forename: Toshiro
authors.initials: T
authors.lastname: Aigaki
authors.forename: Huey
authors.initials: H
authors.lastname: Hing
mesh_terms: D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004330:Drosophila / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D017344:Genes, Insect; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008953:Models, Anatomic; D008957:Models, Genetic; D009833:Olfactory Pathways / Q000033:anatomy & histology / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000502:physiology*
chemical_list: D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; C121679:Akap200 protein, Drosophila; D029721:Drosophila Proteins; D008565:Membrane Proteins