Rapid bidirectional allele-specific PCR identification for triazine resistance in higher plants.
Triazine resistance is reported to be due to chloroplast herbicide target insensitivity in most species, and this is most often caused by a Ser(264)-Gly mutation at the D1 protein. In order to ascertain whether this mutation is really predominant amongst resistant plants, and also for gene flow studies, a rapid test is needed that allows the testing of large quantities of plants. Here a bidirectional allele-specific PCR (polymerase chain reaction) identification is proposed. The designed primers were shown to be universal in the three grass and three broadleaf species examined.
Tian
Xin
X
Unité Mixte de Recherche Biologie et Gestion des Adventices, INRA, BP 86510, F-21065 Dijon, France.
Darmency
Henri
H
D000483:Alleles; D001483:Base Sequence; D004252:DNA Mutational Analysis; D017931:DNA Primers; D004351:Drug Resistance / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006540:Herbicides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D045332:Photosystem II Protein Complex / Q000235:genetics*; D006109:Poaceae; D016133:Polymerase Chain Reaction / Q000379:methods*; D014227:Triazines / Q000494:pharmacology*
D017931:DNA Primers; D006540:Herbicides; D045332:Photosystem II Protein Complex; D014227:Triazines