First successful pregnancy outcome after preimplantation genetic diagnosis for aneuploidy screening in embryos generated from natural-cycle in vitro fertilization combined with an in vitro maturation procedure.
OBJECTIVE
To report on the first successful pregnancy performing preimplantation genetic diagnosis (PGD) on embryos obtained after combining natural IVF with in vitro maturation procedure.


METHODS
Case report.


METHODS
University hospital.


METHODS
A 35-year-old patient with polycystic ovaries who was attending the fertility clinic.


METHODS
In vitro maturation of immature oocytes and preimplantation genetic diagnosis.


METHODS
Preimplantation genetic diagnosis of aneuploidy screening in embryos produced from in vitro maturation procedure.


RESULTS
Thirteen immature and two mature oocytes were collected, and eight mature oocytes were normally fertilized, of which six embryos were biopsied for chromosome analysis. Two chromosomally normal embryos were transferred on day 5, resulting in the birth of a healthy child.


CONCLUSIONS
This case report demonstrates that an acceptable number of embryos can be generated after in vitro maturation procedure to perform PGD for aneuploidy screening and that this approach can be extended to patients with polycystic ovaries or polycystic ovary syndrome who are undergoing PGD for other genetic diseases.
Ao
Asangla
A
Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada. asangla.ao@muhc.mcgill.ca
Jin
Shaoguang
S
Rao
Durga
D
Son
Weon-Young
WY
Chian
Ri-Cheng
RC
Tan
Seang Lin
SL
D000782:Aneuploidy; D001755:Blastocyst; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D050498:Live Birth; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*; D016896:Treatment Outcome