Antiplatelet activity of aporphine and phenanthrenoid alkaloids from Aromadendron elegans Blume.
Six aporphine and one phenanthrenoid alkaloids isolated from Aromadendron elegans Blume were investigated for their ability to inhibit arachidonic acid (AA), collagen and ADP induced platelet aggregation in human whole blood. The antiplatelet activity of the compounds was measured in vitro by the Chrono Log whole blood aggregometer using an electrical impedance method. Of the compounds tested, (-)-N-acetylnornuciferine, (-)-N-acetylanonaine and 1-(N-acetyl-N-methylamino)ethyl-3,4,6-trimethoxy-7-hydroxyphenanthrene showed strong inhibition on platelet aggregation caused by all three inducers. (-)-N-acetylanonaine was the most effective antiplatelet compound as it inhibited both arachidonic acid, collagen and ADP-induced platelet aggregation with IC(50) values of 66.1, 95.1 and 80.6 microm, respectively.
Jantan
Ibrahim
I
Department of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. ibj@medic.ukm.my
Raweh
Salwa M
SM
Yasin
Yusyila Harlina Mohd
YH
Murad
Shahnaz
S
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D001060:Aporphines / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D029562:Magnoliaceae / Q000737:chemistry*; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification*
D000470:Alkaloids; D001060:Aporphines; D010616:Phenanthrenes; D010975:Platelet Aggregation Inhibitors