title: Capillary electrophoretic determination of glucosamine in osteoarthritis tablets via microwave-accelerated dansylation.
abstract: A robust capillary electrophoretic method has been established to separate aminosaccharides including glucosamine, galactosamine, N-methyl-glucamine, N-acetyl-glucosamine and amino glucuronic acid. All the aminosaccharides were dansylated fast under microwave irradiation at 385 W for 6 min (about 50-fold faster than common methods) and detected via on-line UV adsorption at 214 nm. Baseline separation of the dansylatied products was achieved in 20 min using a running buffer of 320 mM borate at pH 9.50. Quantitation of glucosamine in osteoarthritis tablets was then conducted. A linear working range was found in between 2.00 microg/mL and 1.80 mg/mL with linear regression coefficient of 0.9964. The limit of detection reached 1.00 microg/mL glucosamine (signal-to-noise, S/N=3). Recoveries were determined by spiking a known amount of glucosamine in tablet-extracted solutions, giving a range of 88.0-99.7%. The run-to-run relative standard deviation was 0.24% (n=5) for migration time and 2.72% for peak area.
authors.affiliation: Laboratory of Analytical Chemistry for Life Science, Department of Chemical Biology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qi
authors.forename: Shu-Feng
authors.initials: SF
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Zuo
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
mesh_terms: D002021:Buffers; D003619:Dansyl Compounds / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D005944:Glucosamine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D016014:Linear Models; D008872:Microwaves; D008970:Molecular Weight; D010003:Osteoarthritis / Q000188:drug therapy*; D012015:Reference Standards; D015203:Reproducibility of Results; D013607:Tablets
chemical_list: D002021:Buffers; D003619:Dansyl Compounds; D007202:Indicators and Reagents; D013607:Tablets; D005944:Glucosamine