Three-dimensional reconstruction of the ankle by means of ultrathin slice plastination.
Computerized reconstruction of anatomical structures is becoming very useful for developing anatomical teaching modules and animations. Although databases exist consisting of serial sections derived from frozen cadaver material, plastination represents an alternate method for developing anatomical data useful for computerized reconstruction. Plastination is used as an excellent tool for studying different anatomical and clinical questions. The sheet plastination technique is unique because it offers the possibility to produce transparent slices series, which can easily be processed morphometrically. The purpose of this study was to describe a method for developing a computerized model of the human ankle using plastinated slices. This method could be applied to reconstruct any desired region of the human body.A human ankle was obtained, plastinated, sectioned, and subjected to 3D computerized reconstruction using WinSURF modeling system (SURFdriver Software). Qualitative observations revealed that the morphological features of the model were consistent with those displayed by typical cadaveric specimens. Morphometric analysis indicated that the model did not significantly differ from a sample of cadaveric specimens. These data support the use of plastinates for generating tissues sections useful for 3D computerized modeling.
Sora
Mircea-Constantin
MC
Plastination Laboratory, Center for Anatomy and Cell Biology, Medical University of Vienna, Austria. mircea-constantin.sora@meduniwien.ac.at
Genser-Strobl
Birgit
B
Radu
Jilavu
J
Lozanoff
Scott
S
D000842:Ankle / Q000098:blood supply* / Q000294:innervation*; D006801:Humans; D021621:Imaging, Three-Dimensional; D008297:Male; D008867:Microtomy / Q000379:methods*; D016613:Plastic Embedding / Q000379:methods*; D012984:Software; D016909:Tibial Arteries / Q000033:anatomy & histology; D013979:Tibial Nerve / Q000033:anatomy & histology