title: Preliminary HPLC study on some polyphenols of Geranium robertianum L. (Geraniaceae).
abstract: In order to continue our previous studies concerning Geranium robertianum L., herb Robert (Geraniaceae), we have realised a HPLC study of some polyphenols using an original method created by a group of young researchers from the University of Medicine and Pharmacy of Cluj-Napoca. We have identified and measured in the dried Geranii robertiani herba (harvested from Valea Runcului, district of Alba-Iulia) the following compounds: hyperoside (3.64 microg/100 mg), ellagic acid (7599.76 microg/100 mg), isoquercitrin (49.49 microg/100 mg), quercetrin (83.92 microg/100 mg), kaempferols (143.43 microg/100 mg), caftaric acid (166.92 microg/100 mg), rutoside (72.23 microg/100 mg). We have also analysed a hydrolysed sample of the same drug in which we have identified and measured: caffeic acid (6.62 microg/100 mg), ellagic acid (10550.65 microg/100 mg), quercetrin (203.44 microg/100 mg), kaempferols (231.80 microg/100 mg), caftaric acid (47.41 microg/100 mg). We have indirectly proved the presence of ellagic tannins (the amount of ellagic acid increases after acid hydrolise) and the existence of bi- or polycaffeoil derivatives (the caffeic acid is present only in the hydrolysed sample). The flavonoid aglycones exist in both forms: as free compounds and as part of the flavonoid molecules.
authors.affiliation: Ovidius University Constanţa, School of Dental Medicine and Pharmacy, Department of Pharmacology.
authors.forename: Cristina-Stefania
authors.initials: CS
authors.lastname: Fodorea
authors.forename: L
authors.initials: L
authors.lastname: Vlase
authors.forename: Simona
authors.initials: S
authors.lastname: Suciu
authors.forename: M
authors.initials: M
authors.lastname: Tămaş
authors.forename: S E
authors.initials: SE
authors.lastname: Leucuţa
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002109:Caffeic Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004610:Ellagic Acid / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D031315:Geranium / Q000737:chemistry*; D044949:Kaempferols / Q000032:analysis; D010636:Phenols / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D012431:Rutin / Q000031:analogs & derivatives / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D005419:Flavonoids; D044949:Kaempferols; D010636:Phenols; D059808:Polyphenols; C016527:isoquercitrin; C056067:rutin sulfate; D004610:Ellagic Acid; C012526:quercitrin; D012431:Rutin; C021304:hyperoside; D011794:Quercetin; C040048:caffeic acid