title: First report of CTX-M-15 and CTX-M-3 beta-lactamases among clinical isolates of Enterobacteriaceae in Béjaia, Algeria.
abstract: We assessed the prevalence and phenotypic characteristics of extended-spectrum beta-lactamase producers among 365 clinical isolates of members of the family Enterobacteriaceae recovered from two hospitals in Béjaia, Algeria, between March 2004 and April 2005. Twenty-one strains were resistant to cefotaxime and/or ceftazidime. A double-disk synergy test yielded a positive result in five cases (three Escherichia coli, one Klebsiella pneumoniae and one Enterobacter cloacae). Using polymerase chain reaction and sequencing, the three E. coli isolates and the K. pneumoniae isolate were found to produce extended-spectrum beta-lactamase CTX-M-15 and the E. cloacae isolate produced CTX-M-3. The three CTX-M-15-producing E. coli isolates were not isolated in the same wards, although genotyping by randomly amplified polymorphic DNA analysis revealed that they were clonally related. The bla(CTX-M-15) genes were transferred from E. coli by conjugation, whilst conjugative transfer of bla(CTX-M) genes from K. pneumoniae and E. cloacae was not detectable.
authors.affiliation: Laboratoire de Microbiologie Appliquée, FSNV, Université A/MIRA de Béjaia 06000, Algeria. ziz1999@yahoo.fr
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Touati
authors.forename: Said
authors.initials: S
authors.lastname: Benallaoua
authors.forename: Dominique
authors.initials: D
authors.lastname: Forte
authors.forename: Janick
authors.initials: J
authors.lastname: Madoux
authors.forename: Lucien
authors.initials: L
authors.lastname: Brasme
authors.forename: Christophe
authors.initials: C
authors.lastname: de Champs
mesh_terms: D000462:Algeria; D016972:Enterobacter cloacae / Q000201:enzymology*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D004926:Escherichia coli / Q000201:enzymology*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: C473109:beta-lactamase CTX-M-15; C417271:beta-lactamase CTX-M-3; D001618:beta-Lactamases