Isolation and genetic analysis of Japanese encephalitis virus from a diseased horse in Japan.
Japanese encephalitis (JE) developed in an unvaccinated half-bred horse kept in Tottori Prefecture, Japan. The animal showed ataxia with pyrexia and low appetite, and ultimately died. A viral strain was isolated from the cerebrum of the horse and was identified as JE virus (JEV) by RT-PCR using JEV specific primers. The isolated JEV was classified into genotype I by nucleotide sequence analysis of the viral envelope gene. We believe that this is the first report of the genotype I strain being isolated from a horse.
Yamanaka
Takashi
T
Epizootic Research Center, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
Tsujimura
Koji
K
Kondo
Takashi
T
Yasuda
Wataru
W
Okada
Ayako
A
Noda
Kazuomi
K
Okumura
Toshihiro
T
Matsumura
Tomio
T
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000302:isolation & purification; D004672:Encephalitis, Japanese / Q000662:veterinary* / Q000821:virology; D017809:Fatal Outcome; D005838:Genotype; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D007564:Japan; D008297:Male; D051379:Mice; D009500:Neutralization Tests / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012689:Sequence Homology, Nucleic Acid
D000914:Antibodies, Viral