The Schizosaccharomyces pombe cfr1+ gene participates in mating through a new pathway that is independent of fus1+.
Conjugation is a complex event directed to ensure the transfer of genetic material, which is achieved by the union of two cells. In fungi, success of this relevant process requires digestion of the cell wall at the point where both cells have agglutinated and, later, the union of the plasma membranes and nuclei from the mating partners. In order to gain information about cell fusion, we have cloned and disrupted the cfr1+ gene from the fission yeast Schizosaccharomyces pombe. cfr1+ gene is slightly induced at the beginning of mating but Cfr1p protein is degraded soon after the cells are transferred to nitrogen-lacking medium. cfr1Delta mutants present a defect in cell fusion owing to a failure in the digestion of the cell walls between the two parental cells. Finally, cytological and genetic analyses show that cfr1+ acts in a new pathway involved in conjugation that is independent of fus1+, the only gene that has been found to be specifically required for cell fusion during mating in the fission yeast.
Cartagena-Lirola
Hugo
H
Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica, Universidad de Salamanca/CSIC, Edificio Departamental, Campus Miguel de Unamuno, Salamanca, Spain.
Durán
Angel
A
Valdivieso
M-Henar
MH
D015152:Blotting, Northern; D002687:Chitin Synthase / Q000378:metabolism; D003001:Cloning, Molecular; D003227:Conjugation, Genetic / Q000235:genetics*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D049770:Genes, Mating Type, Fungal / Q000502:physiology*; D005964:Glucosyltransferases / Q000378:metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D012568:Schizosaccharomyces / Q000235:genetics* / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics*
D004271:DNA, Fungal; D029702:Schizosaccharomyces pombe Proteins; C490025:fus1 protein, S pombe; D005964:Glucosyltransferases; C040232:glucan synthase; D002687:Chitin Synthase