title: Does Botox effect neural tube development in early chick embryos?
abstract: Botulinum toxin (BTX) is a potent neurotoxin produced by Clostridium botulinum and has wide usage in different areas. The current study aimed to analyze the effects of C. botulinum toxin on the central nerve system in chick embryos. Forty fertile Hubbard Broil eggs, all at Stage 8 of development, were divided into four equal groups: Group 1 embryos (n=10), the control group, were explanted and grown for 18 h in a nutrient medium (thin albumin). Group 2 embryos (n=10) were grown in medium containing 5 U BTX, Group 3 embryos (n=10) in a medium containing 10 U BTX and Group 4 embryos (n=10) in medium containing 20 U BTX. After the incubation period, 80% of Group 1 and 2 embryos and 90% of Group 3 and 4 embryos had intact neural tubes (P>0.05). The results of this study suggest that BTX had no additional effect on neural tube development in early chick embryos.
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Olcay
authors.initials: O
authors.lastname: Eser
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yaman
authors.forename: Emine
authors.initials: E
authors.lastname: Cosar
authors.forename: Abdullah
authors.initials: A
authors.lastname: Konak
authors.forename: Murat
authors.initials: M
authors.lastname: Cosar
authors.forename: Onder
authors.initials: O
authors.lastname: Sahin
authors.forename: Onder
authors.initials: O
authors.lastname: Güney
mesh_terms: D000818:Animals; D019274:Botulinum Toxins, Type A / Q000494:pharmacology*; D002490:Central Nervous System / Q000196:embryology* / Q000473:pathology; D002642:Chick Embryo; D003014:Clostridium botulinum; D047108:Embryonic Development / Q000187:drug effects*; D009498:Neurotoxins / Q000494:pharmacology*
chemical_list: D009498:Neurotoxins; D019274:Botulinum Toxins, Type A