title: [Production purification and characterization of polyantibodies against microcystin-LR].
abstract: OBJECTIVE
  Production and characterization the polyantibodies against MCYST-LR.
  
  
  METHODS
  microcystin-LR (MC-LR) was conjugated to KLH, then immune the rabbit by the routine method. Purify the antiserum by centrifugal and saturated sulfuric ammonium precipitated methods. Count the affinity constant and measure the titers, IC50 Value and the sensitivity.
  
  
  RESULTS
  The titers of the antiserum is over 25600. IC50 value is about from 0.1 ng/ml to 1 ng/ml. The across-reactivity ratio to MC-LW and MC-LR is about from 0.1% to 20%.
authors.affiliation: School of Food Science of Southern Yangtze University, Wuxi 214036, China.
authors.forename: Xiao-Lian
authors.initials: XL
authors.lastname: Zhao
authors.forename: Wei-Rong
authors.initials: WR
authors.lastname: Sun
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xian-Jin
authors.initials: XJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification*; D008297:Male; D008387:Marine Toxins; D052998:Microcystins / Q000276:immunology*; D011817:Rabbits
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR