[Expression of AIV subtype H5HA, H7HA and H9HA hemagglutinin gene in Pichia pastoris].
The expression of the hemagglutinins of Avian influenza virus H5 H7and H9 subtypes was studied in this article by Pichia pastoris, one of the eukaryotis expression systems. Three reconstructed expression plasmids and engineering strains, named pPIC9K-H5HA, pPIC9K-H7HA, pPIC9K-H9HA and GS115/pPIC9K-H5HA, GS115/pPIC9K-H7HA, GS115/pPIC9K-H9HA repectively, were obtained. The reconstructed yeast engineering strains were identified by MD and MM plate selecting and PCR. The induced interests proteins were examined by SDS-PAGE and Western-bloting,the results showed that the interest genes were expressed exactly. And this will be helpful in the future study of antigen detection and antibody detection kit, as well in the subunit vaccines developing.
Xu
Yi-Ming
YM
The Key Genetic Engeneering Lab of PLA Academy of Military Medical Sciences, Changchun 130062, China.
Jin
Ning-Yi
NY
Xia
Zhi-Ping
ZP
Ma
Ming-Xiao
MX
Lu
Hui-Jun
HJ
Han
Song
S
Jin
Kuo-Shi
KS
Liang
Guo-Dong
GD
D000818:Animals; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis / Q000235:genetics*; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics*; D053126:Influenza A Virus, H7N7 Subtype / Q000235:genetics*; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics*; D010843:Pichia / Q000235:genetics / Q000378:metabolism
D019267:Hemagglutinin Glycoproteins, Influenza Virus