Rapid identification of Enterococcus hirae and Enterococcus durans by PCR and detection of a homologue of the E. hirae mur-2 Gene in E. durans.
During an evaluation of PCR for identification of isolates of Enterococcus hirae, a homologue with 82% identity to E. hirae mur-2 was identified in Enterococcus durans and was named mur-2(ed). PCR using primers for two genes (copY and murG) of E. hirae strains showed amplification with E. hirae strains only. PCR (under high-stringency conditions) with primers for the mur-2(ed) gene gave the expected amplification product only with E. durans strains. A combination of murG and mur-2(ed) primers in a multiplex PCR assay differentiated E. hirae from E. durans in all cases. PCR using these primers appears to be a rapid alternative for identification of E. hirae and E. durans isolates.
Arias
Cesar A
CA
Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, TX 77030, USA.
Robredo
Beatriz
B
Singh
Kavindra V
KV
Torres
Carmen
C
Panesso
Diana
D
Murray
Barbara E
BE
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D016983:Enterococcus / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D005798:Genes, Bacterial; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D017351:N-Acetylglucosaminyltransferases; C064057:UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase