title: Deadlock, a novel protein of Drosophila, is required for germline maintenance, fusome morphogenesis and axial patterning in oogenesis and associates with centrosomes in the early embryo.
abstract: The deadlock gene is required for a number of key developmental events in Drosophila oogenesis. Females homozygous for mutations in the deadlock gene lay few eggs and those exhibit severe patterning defects along both the anterior-posterior and dorsal-ventral axis. In this study, we analyzed eggs and ovaries from deadlock mutants and determined that deadlock is required for germline maintenance, stability of mitotic spindles, localization of patterning determinants, oocyte growth and fusome biogenesis in males and females. Deadlock encodes a novel protein which colocalizes with the oocyte nucleus at midstages of oogenesis and with the centrosomes of early embryos. Our genetic and immunohistological experiments point to a role for Deadlock in microtubule function during oogenesis.
authors.affiliation: Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Kristina
authors.initials: K
authors.lastname: Wehr
authors.forename: Andrew
authors.initials: A
authors.lastname: Swan
authors.forename: Trudi
authors.initials: T
authors.lastname: Schüpbach
mesh_terms: D000818:Animals; D019521:Body Patterning; D002455:Cell Division / Q000502:physiology; D018385:Centrosome / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology* / Q000196:embryology* / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology / Q000502:physiology; D005260:Female; D017403:In Situ Hybridization; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D009866:Oogenesis / Q000502:physiology*; D010053:Ovary / Q000166:cytology / Q000502:physiology; D008941:Spindle Apparatus / Q000378:metabolism; D013234:Stem Cells / Q000502:physiology
chemical_list: D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C583035:del protein, Drosophila