title: [Biodegradation of dibutyl phthalate by diatomite adsorptive immobilized microorganism].
abstract: OBJECTIVE
  To study the biodegradation characteristics of seeding type immobilized microorganism on dibutyl phthalate (DBP).
  
  
  METHODS
  The immobilized microorganism was made to adsorb DBP degradation dominant bacteria by using modified diatomite as carrier, then it degraded DBP under different primary concentration, vibration rate, pH, temperature and at the presence of metal compounds. The degradation kinetics was analyzed.
  
  
  RESULTS
  When DBP primary concentration was 100-500 mg/L, the adsorptive immobilized microorganism could maintain relatively high activity and the DBP degraded rate was above 80% in 24h. Dissociative and immobilized microorganism could get higher degradation activity in vibration than in stillness. When pH was 6.0 - 9.0, the degradation rate of immobilized microorganism on DBP was above 82% in 24h and its activity is higher than dissociative microorganism. In the range of 20 degrees C to 40 degrees C, the DBP degraded rate by immobilized microorganism could reach 84.5% in 24h. If mental compounds existed in the DBP water sample, the degradation activities of dissociative and immobilized microorganism were inhibited obviously. The form of DBP degradation kinetics could be described as the first-order model.
  
  
  CONCLUSIONS
  The immobilized microorganisms using diatomite as carrier could degrade DBP effectively. The adsorptive immobilized microorganism was more adapted to DBP load, temperature and pH than dissociative microorganism. The mental compounds could inhibited their activities. The degradation reaction of adsorptive immobilized microorganisms on DBP was according with the first-order model.
authors.affiliation: School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Qi-Fang
authors.initials: QF
authors.lastname: Luo
mesh_terms: D000327:Adsorption; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018914:Cells, Immobilized; D007692:Diatomaceous Earth / Q000737:chemistry*; D003993:Dibutyl Phthalate / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000378:metabolism*; D007700:Kinetics
chemical_list: D052244:Endocrine Disruptors; D003993:Dibutyl Phthalate; D007692:Diatomaceous Earth; C033787:diatomite