Development of the corticospinal tract in the mouse spinal cord: a quantitative ultrastructural analysis.
The growth of corticospinal tract (CST) axons was studied quantitatively at the 7th cervical (C7) and the 4th lumbar (L4) spinal segments in the balb/cByJ mice at the ages of postnatal day (P) 0, 2, 4, 6, 8, 10, 14, and 28. The cross-sectional area of the CST increased progressively with time. Unmyelinated axons, the most prominent CST element during early development, reached maximum at C7 and L4 on P14. Two phases of increase in the number of unmyelinated axons were observed at C7, while only one surge of axonal outgrowth was found at the L4 level. Pro-myelinated axons, defined as axons surrounded by only one layer of oligodendrocytic process, were first seen at P2 and P4 in the C7 and the L4 level, respectively, followed by a dramatic increase in the number of myelinated axons from P14 onwards at both spinal levels. Myelination of the CST axons occurred topographically in a dorsal-to-ventral pattern. The number of growth cones increased rapidly at the C7 level to reach its maximum at P4, while those at L4 increased steadily to the peak at P10. Growth cones with synapse-like junctions were occasionally observed in the growing CST. Degenerating axons and growth cones partly accounted for the massive axon loss at both spinal segments during CST development. Overall, the mouse CST elements changed dynamically in numbers during postnatal development, suggesting a vigorous growing and pruning activity in the tract. The mouse CST also showed a similar growth pattern to that of the rat CST.
Hsu
Jung-Yu C
JY
Department of Anatomy and Neurobiology, Saint Louis University School of Medicine, St. Louis, MO 63108, USA. jung-yu.hsu@ucsf.edu
Stein
Stuart A
SA
Xu
Xiao-Ming
XM
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D007839:Functional Laterality; D020439:Growth Cones / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009412:Nerve Fibers / Q000145:classification / Q000648:ultrastructure; D009413:Nerve Fibers, Myelinated / Q000473:pathology / Q000648:ultrastructure; D036421:Nerve Fibers, Unmyelinated / Q000473:pathology / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology; D011712:Pyramidal Tracts / Q000254:growth & development* / Q000648:ultrastructure*; D013116:Spinal Cord / Q000254:growth & development* / Q000648:ultrastructure*; D013119:Spinal Cord Injuries / Q000473:pathology