title: Campylobacter jejuni strains of human and chicken origin are invasive in chickens after oral challenge.
abstract: The aim of the study was to evaluate the colonizing ability and the invasive capacity of selected Campylobacter jejuni strains of importance for the epidemiology of C jejuni in Danish broiler chickens. Four C. jejuni strains were selected for experimental colonization studies in day-old and 14-day-old chickens hatched from specific pathogen free (SPF) eggs. Of the four C. jejuni strains tested, three were Penner heat-stable serotype 2, flaA type 1/1, the most common type found among broilers and human cases in Denmark. The fourth strain was Penner heat-stable serotype 19, which has been shown to be associated with the Guillain Barré Syndrome (GBS) in humans. The minimum dose for establishing colonization in the day-old chickens was approximately 2 cfu, whereas two- to threefold higher doses were required for establishing colonization in the 14-day-old chickens. Two of the C. jejuni strains were shown to be invasive in orally challenged chickens as well as in three different human epithelial cell lines.
authors.affiliation: Department of Poultry, Fish and Fur Animals, Danish Institute for Food and Veterinary Research, Hangøvej 2, DK-8200 Aarhus N, Denmark.
authors.forename: Katrine Nørrelund
authors.initials: KN
authors.lastname: Knudsen
authors.forename: Dang Duong
authors.initials: DD
authors.lastname: Bang
authors.forename: Lars Ole
authors.initials: LO
authors.lastname: Andresen
authors.forename: Mogens
authors.initials: M
authors.lastname: Madsen
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002169:Campylobacter Infections / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D002460:Cell Line; D002645:Chickens / Q000382:microbiology*; D006367:HeLa Cells; D006801:Humans; D011201:Poultry Diseases / Q000382:microbiology*; D013047:Specific Pathogen-Free Organisms