title: Resveratrol suppresses angiogenesis in gliomas: evaluation by color Doppler ultrasound.
abstract: BACKGROUND
  The effects of resveratrol (a natural polyphenol) on angiogenesis in rat gliomas were investigated by color Doppler ultrasound (CDUS). The correlation among the tumor growth rate, macroscopic angiogenesis measured by CDUS and microscopic angiogenesis was assessed by immunohistochemical staining.
  
  
  METHODS
  Fischer rats were subcutaneously inoculated with rat RT-2 glioma cells and treated with resveratrol for 4 weeks. The tumor size was measured and the animal survival followed. CDUS examination was used to measure tumor blood flow shown as the color Doppler vascularity index (CDVI). Immunohistochemical staining of CD31 was carried out to assess the microvessel density (MVD) of the tumors. The CDVI, MVD and tumor size were correlated.
  
  
  RESULTS
  Rats treated with resveratrol (40 mg/kg/day) had slower tumor growth rates than those of the control groups (p < 0.05). The CDVI, MVD and tumor size were significantly correlated (linear regression, p < 0.0001).
  
  
  CONCLUSIONS
  Resveratrol-suppressed glioma growth was significantly correlated with the inhibition of macroscopic and microscopic angiogenesis.
authors.affiliation: Department of Surgery, Buddhist Tzu Chi Dalin General Hospital, 2, Ming Shen Road, Dalin, Chia-Yi, Taiwan, ROC.
authors.forename: Jin-Cherng
authors.initials: JC
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Jyh-Herng
authors.initials: JH
authors.lastname: Lin
authors.forename: Jiann-Ming
authors.initials: JM
authors.lastname: Wu
authors.forename: Sheng-Hong
authors.initials: SH
authors.lastname: Tseng
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D001932:Brain Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000473:pathology; D048708:Cell Growth Processes / Q000187:drug effects; D005910:Glioma / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging / Q000188:drug therapy; D051381:Rats; D011916:Rats, Inbred F344; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D018615:Ultrasonography, Doppler, Color
chemical_list: D020533:Angiogenesis Inhibitors; D013267:Stilbenes; D000077185:Resveratrol