Effects of sodium arsenite on catalase activity, gene and protein expression in HaCaT cells.
The objective of this research work is to study the effects of sodium arsenite on activity, mRNA and protein expression of catalase (CAT) in established human cell lines of keratinocytes (HaCaT). Based on the AlamarBlue assay, we found that a high level (100 micromol/l) of sodium arsenite inhibited cell viability. The reactive oxygen species (ROS) content in cells was increased in all treated cultures dose-dependently. CAT activity, mRNA expression and protein levels were decreased by 5-20 micromol/l of sodium arsenite. It is highly suggestive that CAT gene expression and protein levels are affected by arsenic.
Sun
Xiance
X
Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang, Liaoning, PR China.
Li
Bing
B
Li
Xin
X
Wang
Yi
Y
Xu
Yuanyuan
Y
Jin
Yaping
Y
Piao
Fengyuan
F
Sun
Guifan
G
D018053:Arsenites / Q000494:pharmacology*; D002374:Catalase / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017670:Sodium Compounds / Q000494:pharmacology*
D018053:Arsenites; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D017670:Sodium Compounds; C017947:sodium arsenite; D002374:Catalase