Analysis of aromatase and 17beta-hydroxysteroid dehydrogenase type 2 messenger ribonucleic acid expression in deep endometriosis and eutopic endometrium using laser capture microdissection.
OBJECTIVE
To investigate mRNA expression of aromatase and 17beta-hydroxysteroid dehydrogenase type 2 (17betaHSD2) in epithelial and stromal cells from eutopic and ectopic endometrium of patients with deep endometriosis.


METHODS
Prospective study.


METHODS
University hospital.


METHODS
Patients with deep endometriosis and fertile women with macroscopically normal pelvic cavities.


METHODS
During surgery, 30 endometrial and 16 endometriotic samples were obtained from 30 patients with deep endometriosis. Control endometrial samples were obtained from 24 fertile women with macroscopically normal pelvic cavities who underwent laparoscopic tubal ligation or reversal of tubal sterilization. Epithelial cells and stromal cells from endometrial or endometriotic tissues were microdissected using laser capture microdissection.


METHODS
Expression levels of aromatase and 17betaHSD2 mRNA in microdissected epithelial and stromal cells were determined using quantitative real-time reverse transcriptase polymerase chain reaction.


RESULTS
Aromatase mRNA expression was significantly higher in epithelial cells than in stromal cells in both eutopic and ectopic endometrium obtained from endometriosis patients. In the ectopic endometrium of 8 patients (8/16, 50%), 17betaHSD2 expression was not detected in either epithelial or stromal cells. In eutopic endometrium from endometriosis patients, 17betaHSD2 expression in epithelial cells was significantly increased during the early, middle, and late secretory phases compared with the late proliferative phase, whereas no significant cyclical difference was detected in control endometrium.


CONCLUSIONS
Local estrogen concentration may be much higher in epithelial cells than in stromal cells in deep endometriotic tissue.
Matsuzaki
Sachiko
S
Department of Gynecology, Polyclinique de l'Hôtel-Dieu, CHU, Clermont-Ferrand, France. sachikoma@aol.com
Canis
Michel
M
Pouly
Jean-Luc
JL
Déchelotte
Pierre J
PJ
Mage
Gérard
G
D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D001141:Aromatase / Q000378:metabolism*; D004715:Endometriosis / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D004717:Endometrium / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D004847:Epithelial Cells / Q000201:enzymology; D004960:Estradiol Dehydrogenases; D005260:Female; D005498:Follicular Phase; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D053685:Laser Therapy; D008183:Luteal Phase; D042282:Microdissection; D011446:Prospective Studies; D012333:RNA, Messenger / Q000378:metabolism*; D017154:Stromal Cells / Q000201:enzymology; D014018:Tissue Distribution
D007527:Isoenzymes; D012333:RNA, Messenger; D015067:17-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases; C523763:HSD17B2 protein, human; D001141:Aromatase