title: The effect of hypoxia on photocytotoxicity of TICS tricarbocyanine dye in vitro.
abstract: OBJECTIVE
  To evaluate the effect of cell oxygenation on photocytotoxicity of a novel tricarbocyanine indolenine dye covalently bound to glucose (TICS).
  
  
  METHODS
  HeLa cells were incubated with 5 microM TICS, 2 h later irradiated by laser at 740 nm with a light dose of 10 J/cm(2), delivered at a power density of 10, 20, 25 or 30 mW/cm(2), in air or in argon atmosphere, and then scored for viability.
  
  
  RESULTS
  The photocytotoxicity of TICS increased dramatically as the power density was reduced. Under hypoxia TICS-photosensitized cell death was determined but its value was lowered, compared to photoirradiation in the air.
  
  
  CONCLUSIONS
  Photosensitizing effect of TICS is only partially dependent on the oxygenation of tumor cells.
authors.affiliation: NN Alexandrov Research Institute of Oncology and Medical Radiology, Minsk, Belarus. Istomin@users.med.by
authors.forename: Y P
authors.initials: YP
authors.lastname: Istomin
authors.forename: E N
authors.initials: EN
authors.lastname: Alexandrova
authors.forename: E A
authors.initials: EA
authors.lastname: Zhavrid
authors.forename: E S
authors.initials: ES
authors.lastname: Voropay
authors.forename: M P
authors.initials: MP
authors.lastname: Samtsov
authors.forename: K N
authors.initials: KN
authors.lastname: Kaplevsky
authors.forename: A P
authors.initials: AP
authors.lastname: Lugovsky
authors.forename: A A
authors.initials: AA
authors.lastname: Lugovsky
mesh_terms: D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D010778:Photochemotherapy / Q000379:methods