A soluble LAG-3 protein as an immunopotentiator for therapeutic vaccines: Preclinical evaluation of IMP321.
A soluble LAG-3 (CD223) molecule (sLAG-3) is a natural high affinity ligand for MHC class II. It is known to induce maturation of monocyte-derived dendritic cells in vitro and is used as a vaccine adjuvant to induce CD4 Th1 and CD8 T cell responses in vivo. The work presented herein was performed to study the biological activity of a preclinical grade human sLAG-3 protein, called IMP321: (i) binding on murine APC, (ii) induction of dendritic cell maturation, (iii) safety of repeated injections in mice. The results show that IMP321 binds MHC class II(+) murine APC, induces the maturation of dendritic cells and is well tolerated either injected alone or as an adjuvant with strongly immunogenic antigens.
Fougeray
Sylvie
S
Immutep S.A., Parc Club Orsay, 2 Rue Jean Rostand 91893 Orsay, France.
Brignone
Chrystelle
C
Triebel
Frédéric
F
D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000494:pharmacology*; D000818:Animals; D015703:Antigens, CD / Q000008:administration & dosage* / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005802:Genes, MHC Class II; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011485:Protein Binding; D014612:Vaccines / Q000276:immunology*; D000093322:Lymphocyte Activation Gene 3 Protein
D000276:Adjuvants, Immunologic; D015703:Antigens, CD; D014612:Vaccines; D000093322:Lymphocyte Activation Gene 3 Protein