title: Polymerase chain reaction identification of a hymenopteran insect in the cornea: a case report.
abstract: The type of corneal injuries associated with insect encounters is related to the composition of the foreign body. However, previous reports on corneal foreign bodies as insects were rarely based on scientific evidence. Here, we report on a 49-year-old male who was stung in his left eye by an unknown insect. Emergent keratotomy was performed to remove the embedded corneal foreign body. The removed foreign body was observed under light microscopy, and a fragment of insect was suspected. The sample was sent for molecular analysis. The polymerase chain reaction product was sequenced, subjected to a BLAST search, and identified as an ichneumonoid member of the insect order Hymenoptera.
authors.affiliation: Department of Ophthalmology, Kaohsiung Medical University Hospital, Taiwan.
authors.forename: Hsien-Chung
authors.initials: HC
authors.lastname: Lin
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Yeh
authors.forename: Cheng-Hsien
authors.initials: CH
authors.lastname: Chang
authors.forename: Yaw-Syan
authors.initials: YS
authors.lastname: Fu
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Cheng
authors.forename: Horng-Jiun
authors.initials: HJ
authors.lastname: Wu
authors.forename: Hwei-Zu
authors.initials: HZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D065306:Corneal Injuries; D005129:Eye Foreign Bodies / Q000175:diagnosis*; D006801:Humans; D006927:Hymenoptera / Q000235:genetics*; D007299:Insect Bites and Stings / Q000150:complications*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*