title: Matrix metalloproteinase and tissue inhibitor of metalloproteinase in serum and synovial fluid of osteoarthritic dogs.
abstract: To better understand the mechanisms responsible for the pathological processes of osteoarthritis (OA) and to potentially identify a profile of changes that could be predictive of early OA, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in the synovial fluid and serum of normal and osteoarthritic dogs were examined. The concentration of MMP-1 in the synovial fluid of osteoarthritic dogs (0.62 +/- 0.16), as measured by densitometry, was significantly higher than that found in control dogs (0.42 +/- 0.19) (P = 0.03). The concentration of MMP-1 in the serum of osteoarthritic dogs (0.74 +/- 0.16) was significantly less than that found in control dogs (0.87 +/- 0.08) (P = 0.05). The concentration of TIMP-2 in the synovial fluid of osteoarthritic dogs (46.2 +/- 21.9 ng/ml) was significantly less than that of control dogs (122.0 +/- 66.5 ng/ml) (P = 0.009). The concentration of TIMP-2 in the serum of osteoarthritic dogs (116.2 +/- 43.1 ng/ml) was not significantly different than that of control dogs (95.1 +/- 94.4 ng/ml) (P = 0.554). In addition, a phospho-tyrosine immunoprecipitation and mass spectrometry were used to isolate and identify interferon-alpha in canine synovial fluid.
authors.affiliation: Department of Clinical Science, Kansas State University, Manhattan 66506, USA. bsalinar@vet.ksu.edu
authors.forename: B J
authors.initials: BJ
authors.lastname: Salinardi
authors.forename: J K
authors.initials: JK
authors.lastname: Roush
authors.forename: T
authors.initials: T
authors.lastname: Schermerhorn
authors.forename: K E
authors.initials: KE
authors.lastname: Mitchell
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D016022:Case-Control Studies; D004283:Dog Diseases / Q000097:blood / Q000201:enzymology* / Q000378:metabolism; D004285:Dogs; D005260:Female; D016898:Interferon-alpha / Q000032:analysis; D008297:Male; D045726:Metalloproteases / Q000032:analysis*; D010003:Osteoarthritis / Q000097:blood / Q000201:enzymology / Q000378:metabolism / Q000662:veterinary*; D013582:Synovial Fluid / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis*
chemical_list: D016898:Interferon-alpha; D019716:Tissue Inhibitor of Metalloproteinase-2; D045726:Metalloproteases