A recombinant pseudorabies virus encoding the HA gene from H3N2 subtype swine influenza virus protects mice from virulent challenge.
The hemagglutinin (HA) gene of A/Swine/Inner Mogolian/547/2001 (H3N2) swine influenza virus (SIV) was recombined into the genome of pseudorabies virus (PRV) Bartha-K61 vaccine strain, generating a recombinant PRV expressing the HA gene, designated as rPRV-HA. One group of 15 mice was inoculated intranasally (i.n.) with 10(5.0) PFU of rPRV-HA, and another two control groups of mice (15 mice per group) were mock-inoculated or inoculated with Bartha-K61. Mice inoculated with rPRV-HA developed hemagglutination inhibition antibodies 3 weeks post-inoculation. Twenty-eight days post-inoculation, all mice were challenged i.n. with 10(5.0) TCID50 of A/Swine/Heilongjiang/74/2000 (H3N2). No challenge virus was isolated from vaccinated mice, and mild pathological lesions were observed only in lungs following challenge. The results demonstrate that the recombinant rPRV-HA expressing the HA gene from H3N2 SIV can protect mice from heterologous virulent challenge, and may represent a candidate vaccine against SIV.
Tian
Zhi-Jun
ZJ
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
Zhou
Guo-Hui
GH
Zheng
Bao-Liang
BL
Qiu
Hua-Ji
HJ
Ni
Jian-Qiang
JQ
Yang
Huan-Liang
HL
Yin
Xun-Nan
XN
Hu
Shou-Ping
SP
Tong
Guang-Zhi
GZ
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology*; D011558:Herpesvirus 1, Suid / Q000235:genetics; D006651:Histocytochemistry / Q000662:veterinary; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D008171:Lung Diseases / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D051379:Mice; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary* / Q000821:virology; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology*; D014611:Vaccination / Q000662:veterinary; D014709:Vero Cells
D000914:Antibodies, Viral; D006388:Hemagglutinins; D007252:Influenza Vaccines