Qualitative identification of doxacurium and its breakdown products in postmortem fluids by liquid chromatography-tandem mass spectrometry.
During a death investigation at the Office of the Cuyahoga County Coroner in Cleveland, OH, doxacurium became a drug of interest. The Coroner's Office enlisted the aid of the Federal Bureau of Investigation Laboratory for the doxacurium analysis. Following the request, a method for the extraction and qualitative analysis of the drug in biological fluids was developed. The procedure relies on a simple solid-phase extraction procedure followed by qualitative analysis with liquid chromatography-tandem mass spectrometry. During the development of the new analytical procedure, two breakdown products of doxacurium were detected. Structures for these breakdown products are proposed. This procedure was used to analyze heart blood, cerebrospinal fluid, and bile specimens from the decedent. Doxacurium and its breakdown products were identified in all three specimens.
Montgomery
Madeline A
MA
Chemistry Unit, Federal Bureau of Investigation Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USA. Madeline.Montgomery@ic.fbi.gov
LeBeau
Marc A
MA
Jenkins
Amanda J
AJ
D002555:Cerebrospinal Fluid / Q000737:chemistry*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D007546:Isoquinolines / Q000032:analysis* / Q000378:metabolism; D009467:Neuromuscular Depolarizing Agents / Q000032:analysis* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013405:Suicide
D007546:Isoquinolines; D009467:Neuromuscular Depolarizing Agents; C049429:doxacurium