title: Characterization of human D-amino acid oxidase.
abstract: D-Amino acid oxidase (DAAO) has been proposed to be involved in the oxidation of D-serine, an allosteric activator of the NMDA-type glutamate receptor in the brain, and to be associated with the onset of schizophrenia. The recombinant human DAAO was expressed in Escherichia coli and was isolated as an active homodimeric flavoenzyme. It shows the properties of the dehydrogenase-oxidase class of flavoproteins, possesses a low kinetic efficiency, and follows a ternary complex (sequential) kinetic mechanism. In contrast to the other known DAAOs, the human enzyme is a stable homodimer even in the apoprotein form and weakly binds the cofactor in the free form.
authors.affiliation: Department of Biotechnology and Molecular Sciences, University of Insubria, Varese, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Molla
authors.forename: Silvia
authors.initials: S
authors.lastname: Sacchi
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Bernasconi
authors.forename: Mirella S
authors.initials: MS
authors.lastname: Pilone
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Fukui
authors.forename: Loredano
authors.initials: L
authors.lastname: Polegioni
mesh_terms: D001921:Brain / Q000201:enzymology; D003067:Coenzymes / Q000737:chemistry / Q000378:metabolism; D003605:D-Amino-Acid Oxidase / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D020536:Enzyme Activators / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D012559:Schizophrenia / Q000201:enzymology; D012694:Serine / Q000737:chemistry / Q000378:metabolism
chemical_list: D003067:Coenzymes; D020536:Enzyme Activators; D016194:Receptors, N-Methyl-D-Aspartate; D012694:Serine; C417983:DAO protein, human; D003605:D-Amino-Acid Oxidase