The structural basis for the serospecificity of Actinobacillus suis serogroup O:2.
Actinobacillus suis is an important bacterial pathogen of healthly pigs. An O-antigen (lipopolysaccharide; LPS) serotyping system is being developed to study the prevalence and distribution of representative isolates from both healthy and diseased pigs. In a previous study, we reported that A. suis serogroup O:1 strains express LPS with a (1-->6)-beta-D-glucan O-antigen chain polysaccharide that is similar in structure to a key cell-wall component in yeasts, such as Saccharomyces cerevisiae and Candida albicans. This study describes the O-antigen polysaccharide chemical structure of an O:2 serogroup strain, A. suis H91-0380, which possesses a tetrasaccharide repeating block with the structure: -->3)-beta-D-Galp-(1-->4)-[alpha-D-Galp-(1-->6)]-beta-D-Glcp-(1-->6)-beta-D-GlcpNAc-(1-->. Studies have shown that A. suis serogroup O:2 strains are associated with severely diseased animals; therefore, work on the synthesis of a glycoconjugate vaccine employing O:2 O-antigen polysaccharide to vaccinate pigs against A. suis serogroup O:2 strains is currently underway.
Rullo
Anthony
A
Department of Chemistry, University of Guelph, Canada.
Papp-Szabo
Erzsebet
E
Michael
Frank St
FS
Macinnes
Janet
J
Monteiro
Mario A
MA
D000189:Actinobacillus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D044132:Actinobacillus suis / Q000737:chemistry* / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D012703:Serotyping; D016339:Spectrometry, Mass, Fast Atom Bombardment; D034421:Sus scrofa; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control; D014774:Virulence / Q000276:immunology
D001428:Bacterial Vaccines; D019081:O Antigens