Isolation and characterization of novel denitrifying alkalithermophiles, AT-1 and AT-2.
Two novel denitrifying alkalithermophilic bacteria, AT-1 and AT-2, were isolated from manure-amended soil. The isolates grew at 35-65 degrees C with an optimum temperature at 50-60 degrees C, and pH 6.5-10.0 with an optimum pH at 9.5. Both isolates were Gram-positive, facultative anaerobic, non-motile rod-shaped bacteria. A phylogenetic analysis based on 16S rRNA sequence data indicated that both AT-1 and AT-2 are members of the genus Anoxybacillus. DNA-DNA hybridization revealed moderate relatedness between AT-1 and AT-2 and one phylogenetically related strain, A. pushchinensis K1 (69.5 and 69.1%, respectively). Comparative analysis of morphology and biochemical characteristics of the two isolates also showed similarity to A. pushchinensis K1. Based on these results, we identified AT-1 and AT-2 as A. pushchinensis. To our knowledge, this is the first report of denitrifying bacterium isolated from alkalithermophilic Anoxybacillus spp.
Yamamoto
Mami
M
REDS Group (Saitama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST), Saitama Small Enterprise Promotion Corporation, 3-12-18 Kamiaoki, Kawaguchi, Saitama 333-0844, Japan.
Ishii
Akihiro
A
Nogi
Yuichi
Y
Inoue
Akira
A
Ito
Masahiro
M
D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D010641:Phenotype; D010802:Phylogeny; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009566:Nitrates