title: The p67 laminin receptor identifies human erythroid progenitor and precursor cells and is functionally important for their bone marrow lodgment.
abstract: The laminins are a group of extracellular matrix proteins with constitutive expression in all tissues, including bone marrow stroma. A functional role for the nonintegrin laminin receptor p67 has been described for cancer metastasis and lymphocyte trafficking. Expression of p67 was also reported for other subsets of mature leukocytes and for malignant hematopoietic or nonhematopoietic cells. We explored p67 expression on normal hematopoietic progenitor cells (HPCs) and its putative role in bone marrow retention of transplanted HPCs. We found p67 expression on a subset of primary human CD34(+) cells coexpressing erythroid markers. Of importance, p67 recognizes early erythroid progenitors, since sorted p67(+) cells were significantly enriched for burst-forming units-erythroid (BFU-Es) and depleted of colony-forming units--granulocyte/macrophage (CFU-GMs). Blockade of p67 binding of donor cells, using antifunctional antibody, reduced bone marrow homing of BFU-Es. These studies identify p67 as a novel phenotypic marker for erythroid HPCs of functional importance for lineage-specific homing/retention among adult transplanted HPCs.
authors.affiliation: University of Washington, Departmen of Medicine/Hematology, Seattle, WA 98195-7720, USA. hbonig@u.washington.edu
authors.forename: Halvard
authors.initials: H
authors.lastname: Bonig
authors.forename: Kai-Hsin
authors.initials: KH
authors.lastname: Chang
authors.forename: Betty
authors.initials: B
authors.lastname: Nakamoto
authors.forename: Thalia
authors.initials: T
authors.lastname: Papayannopoulou
mesh_terms: D000328:Adult; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D018952:Antigens, CD34 / Q000096:biosynthesis*; D001853:Bone Marrow / Q000378:metabolism; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000378:metabolism* / Q000637:transplantation; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D042381:Granulocyte Precursor Cells / Q000378:metabolism; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D009369:Neoplasms / Q000378:metabolism; D017474:Receptors, Laminin / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D000906:Antibodies; D018952:Antigens, CD34; D017474:Receptors, Laminin