title: Large-scale identification of protein-protein interaction of Escherichia coli K-12.
abstract: Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested, partners were found for 2667, including 779 of unknown function. Proteins copurifying with hexahistidine-tagged baits on a Ni2+-NTA column were identified by MALDI-TOF MS (matrix-assisted laser desorption ionization time of flight mass spectrometry). An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Arifuzzaman
authors.forename: Maki
authors.initials: M
authors.lastname: Maeda
authors.forename: Aya
authors.initials: A
authors.lastname: Itoh
authors.forename: Kensaku
authors.initials: K
authors.lastname: Nishikata
authors.forename: Chiharu
authors.initials: C
authors.lastname: Takita
authors.forename: Rintaro
authors.initials: R
authors.lastname: Saito
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ara
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakahigashi
authors.forename: Hsuan-Cheng
authors.initials: HC
authors.lastname: Huang
authors.forename: Aki
authors.initials: A
authors.lastname: Hirai
authors.forename: Kohei
authors.initials: K
authors.lastname: Tsuzuki
authors.forename: Seira
authors.initials: S
authors.lastname: Nakamura
authors.forename: Mohammad
authors.initials: M
authors.lastname: Altaf-Ul-Amin
authors.forename: Taku
authors.initials: T
authors.lastname: Oshima
authors.forename: Tomoya
authors.initials: T
authors.lastname: Baba
authors.forename: Natsuko
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Kawamura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ioka-Nakamichi
authors.forename: Masanari
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Kanaya
authors.forename: Chieko
authors.initials: C
authors.lastname: Wada
authors.forename: Hirotada
authors.initials: H
authors.lastname: Mori
mesh_terms: D048168:Escherichia coli K12 / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D015723:Gene Library; D006639:Histidine / Q000737:chemistry; D008954:Models, Biological; D016366:Open Reading Frames; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D029968:Escherichia coli Proteins; D020543:Proteome; D006639:Histidine