title: Cationic amylopectin derivatives as additives for analysis of proteins in capillary electrophoresis.
abstract: Positively charged amylopectin, which is a major constituent of cationic starch, was used to modify the inner surface of fused-silica capillaries by addition to the running solution, which was subsequently employed in CE. Capillaries filled with cationic amylopectin derivatives were shown to generate a stable reversed EOF in the investigated range of pH 4-8. Among the additives studied, quaternary ammonium amylopectin derivatives with high amino and low hydroxypropyl groups showed fast electroosmotic mobility and very effectively suppressed the adsorption of proteins. The run-to-run and batch-to-batch repeatability of the procedures were satisfactory with RSDs of 0.5% and 2.4%, respectively. A basic protein, alpha-chymotrypsinogen, migrated within 6 min and the theoretical plate number of it reached 560 000 plates/m.
authors.affiliation: Department of Analytical Chemistry, School of Pharmaceutical Sciences and COE Program in the 21st Century, University of Shizuoka, Shizuoka, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Kato
authors.forename: Eriko
authors.initials: E
authors.lastname: Imamura
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sakai-Kato
authors.forename: Tohru
authors.initials: T
authors.lastname: Nakajima
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Toyo'oka
mesh_terms: D000687:Amylopectin / Q000031:analogs & derivatives* / Q000737:chemistry; D002412:Cations; D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000032:analysis*
chemical_list: D002412:Cations; D011506:Proteins; D000687:Amylopectin