Suppression of disease phenotypes of adult mito-mice carrying pathogenic mtDNA by bone marrow transplantation.
For directly addressing the issue of gene therapy of adult patients with mitochondrial diseases, we carried out bone marrow transplantation to adult mito-mice with mutated mtDNA and expressing respiration defects for improvement of disease phenotypes. We supposed that bone marrow cells transdifferentiated into various tissues, so that their transplantation would suppress disease phenotypes. The results showed improvement of survival and delayed expression of renal failure. As most mito-mice without a transplant died due to renal failure, we examined whether transplanted bone marrow cells transdifferentiated into renal tissues carrying improved renal function. Histochemical analyses showed that the suppression of disease phenotypes was not due to transdifferentiation, but due to suppression of apoptosis of renal cells. Thus, bone marrow cells possess a novel function of supporting tissues by suppressing apoptosis induced by respiration defects.
Inoue
Shin-Ichi
S
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.
Ishikawa
Kaori
K
Nakada
Kazuto
K
Sato
Akitsugu
A
Miyoshi
Hiroyuki
H
Hayashi
Jun-Ichi
J
D000818:Animals; D017209:Apoptosis; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation; D004272:DNA, Mitochondrial / Q000378:metabolism; D015316:Genetic Therapy / Q000379:methods; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D010641:Phenotype; D051437:Renal Insufficiency / Q000378:metabolism
D004272:DNA, Mitochondrial