Design and synthesis of a biotin-tagged photoaffinity probe of paeoniflorin.
A trifunctional probe (binding element-photoreactive group-affinity tag) of natural product paeoniflorin was designed and synthesized based on the previous primary structure-activity relationship. This new probe is a potential tool for labeling, purification, and identification of the target proteins.
Qiu
Wen-Wei
WW
Chinese National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, 189 Guo-Shou-Jing Road, Shanghai 201203, China.
Xu
Jie
J
Liu
Da-Zhi
DZ
Li
Jing-Ya
JY
Ye
Yang
Y
Zhu
Xing-Zu
XZ
Li
Jia
J
Nan
Fa-Jun
FJ
D000818:Animals; D001565:Benzoates / Q000138:chemical synthesis / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D001952:Bridged-Ring Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D015195:Drug Design; D005960:Glucosides / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D039821:Monoterpenes; D019920:Photoaffinity Labels / Q000737:chemistry*; D051381:Rats; D006027:Glycosides
D001565:Benzoates; D001710:Biotin; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; D019920:Photoaffinity Labels; C015423:peoniflorin; D006027:Glycosides