title: Differential restoration of myeloid and plasmacytoid dendritic cells in HIV-1-infected children after treatment with highly active antiretroviral therapy.
abstract: Numerical and functional deficits in myeloid (mDC) and plasmacytoid dendritic cell (pDC) subsets have been found in both adult and pediatric HIV-1 carriers. Whether these impaired DC subsets can be restored after treatment with highly active antiretroviral therapy (HAART) is currently unknown, especially in HIV-1-infected children. In this report, we characterized mDC and pDC subsets in 18 HIV-1-infected children who received HAART treatment and compared them with those in 6 untreated HIV-1-infected children and 27 HIV-1-uninfected healthy children. Among children treated with HAART, 11 were found to suppress HIV-1 replication successfully below the detection limit (HAART-suppressed group) while the remaining 7 failed (HAART-failure group). In HAART-suppressed children, a gradual and complete restoration of the frequency and function of mDCs was observed while the recovery of pDCs was only partial. However, mDC and pDC subsets in HARRT failure children were indistinguishable from the HAART-naive infected children. We also found that mDC frequency and IFN-alpha-releasing capacity of pDC positively correlated with CD4 T cell percentages in all HIV-1-infected children. In HAART-naive children, the mDC frequency correlated the HIV-1-specific CTL frequency. Our findings suggest that HAART has a differential impact on the restoration of mDC and pDC subsets. These findings may help guide the development of HIV-1-specific immune therapy aimed at fully restoring host immune function in chronically HIV-1-infected children.
authors.affiliation: Research Center of Biological Therapy, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Beijing 100039, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Junliang
authors.initials: J
authors.lastname: Fu
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Zhao
authors.forename: Yun
authors.initials: Y
authors.lastname: He
authors.forename: Lei
authors.initials: L
authors.lastname: Jin
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Jinxia
authors.initials: J
authors.lastname: Yao
authors.forename: Linqi
authors.initials: L
authors.lastname: Zhang
authors.forename: Fu-Sheng
authors.initials: FS
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D023241:Antiretroviral Therapy, Highly Active; D018791:CD4 Lymphocyte Count; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D005260:Female; D015658:HIV Infections / Q000188:drug therapy* / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology; D008297:Male; D022423:Myeloid Cells / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology