title: [Enhanced-real time PCR: a highly sensitive method for SARS-coronavirus detection].
abstract: An enhanced real-time polymerase chain reaction (ERT-PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-Cov) has been designed for detection of SARS-Cov with high sensitivity and easy-to-interpret results, in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. The limit of detection of the ERT-PCR method was 10(-2) higher than the standard real-time PCR assay and 10(-7) higher than conventional PCR methods. The increased sensitivity of the assay would have major benefits in screening suspected SARS patients rapidly and efficiently and may help control the spread of SARS and other infectious diseases during future outbreak.
authors.affiliation: Neuroscience Research Institue, Peking University, Beijing 100083, China. achy@bjmu.edu.cn
authors.forename: Chang-hai
authors.initials: CH
authors.lastname: Yu
authors.forename: Le-ting
authors.initials: LT
authors.lastname: Liu
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Yan-yun
authors.initials: YY
authors.lastname: Feng
authors.forename: Chen-ran
authors.initials: CR
authors.lastname: Wang
authors.forename: Hui-li
authors.initials: HL
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Ji-sheng
authors.initials: JS
authors.lastname: Han
mesh_terms: D006801:Humans; D057230:Limit of Detection; D012367:RNA, Viral / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral