title: Proteomic analysis of the transition from quiescent to proliferating stages in rat liver hepatectomy model.
abstract: The 70% (or 2/3) partial hepatectomy (PHx) rat liver model provides an effective medium for study of the transition and regulation of hepatocytes from quiescent to proliferating phase. Although the gene expression pattern has come under intense scrutiny, a differential proteomic study could help to reveal the mechanism of how the process is initiated and regulated. The proteomic changes were analyzed in two groups, 7 h after 70% PHx test group and sham-operation control group, by two-dimensional gel electrophoresis with 907 +/- 33 and 910 +/- 64 spots on gels, respectively. Twelve down-regulated spots and twenty-six up-regulated spots were recognized using ImageMaster software and were identified by matrix-assisted laser desorption/ionization-mass spectrometry-quadrupole time of flight mass spectrometry and/or tandem mass spectrometry reconfirmation. Some of the differential proteins were associated with stress defense, lipid metabolism, and macromolecular biosynthesis while the others were shown to be involved in regulating transcript factors associated with liver regeneration. A "proteomic model" for liver regeneration was suggested based on our data and related scientific literature to interpret the differential proteome pattern that reflected the transition of cells from quiescent to proliferating state, including but not limited to the rat liver after 70% PHx.
authors.affiliation: Institute of Cell Biology and Key Laboratory for Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University, Beijing, P.R. China 100875.
authors.forename: Fuzheng
authors.initials: F
authors.lastname: Guo
authors.forename: Hui
authors.initials: H
authors.lastname: Nian
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Lingyun
authors.initials: L
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Xiao
authors.forename: Dacheng
authors.initials: D
authors.lastname: He
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D006498:Hepatectomy; D022781:Hepatocytes / Q000378:metabolism / Q000502:physiology; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D008115:Liver Regeneration; D020543:Proteome / Q000096:biosynthesis*; D051381:Rats; D016192:Resting Phase, Cell Cycle; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome