Differential glycosylation of rhLf expressed in the mammary gland of transgenic mice.
Differential glycosylation of natural hLf and rhLf from hLf-transgenic mice, which harbored a 146 Kb BAC insert that includes the intact hLf gene sequence, was studied in the present report. There were significant differences between the immunoblotting results of rhLf and natural hLf, which were denatured with nonreducing SDS sample buffer. The differences disappeared after rhLf and natural hLf samples were digested with N-glycosidase F, respectively. The results showed that there were significant differences (P<0.01) between the glycosylation of natural hLf and rhLf that were purified, respectively, from milk samples of seven hLf-transgenic mouse lines.
Zhao
Chunjiang
C
Key Laboratories of Ministry of Agriculture of P.R. China for Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing, P.R. China.
Liu
Zhaoliang
Z
Fan
Baoliang
B
Dai
Yunping
Y
Wang
Lili
L
Zheng
Min
M
Wang
Meili
M
Niu
Huiling
H
Xi
Fengong
F
Li
Ning
N
Zhang
Dinglian
D
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006031:Glycosylation; D006801:Humans; D015151:Immunoblotting; D007781:Lactoferrin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008892:Milk / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
D011994:Recombinant Proteins; D007781:Lactoferrin; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase