Lactose-induced production of human soluble B lymphocyte stimulator (hsBLyS) in E. coli with different culture strategies.
Over-production of human soluble B lymphocyte stimulator (hsBLyS) was carried out with four different fed-batch culture strategies using lactose as inducer, instead of IPTG, in a fed-batch culture of Escherichia coli. As lactose acted as both inducer and carbon source, the best and simplest culture strategy was direct feeding of lactose after batch culture, thereby giving hsBLyS at 3.7 g l(-1) and a productivity of 0.11 g l(-1) h(-1).
Li
Zhaopeng
Z
College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, PR China.
Zhang
Xu
X
Tan
Tianwei
T
D053264:B-Cell Activating Factor / Q000235:genetics* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007785:Lactose / Q000008:administration & dosage* / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism
D053264:B-Cell Activating Factor; D011994:Recombinant Proteins; D007785:Lactose