title: [Screening methods for detection of metallo-beta-lactamase producing gram negative rods].
abstract: Modified Hodge test and a method using a disk with imipenem plus 1000 mg of EDTA were used to determine the presence of metallo-beta-lactamase producing gram-negative rods among 166 clinical isolates from hospitals in Iaşi and Galaţi. Of 9 imipenem resistant strains found, only one Pseudomonas aeruginosa gave positive results with both tests and other two P. aeruginosa clinical isolates gave negative results with both tests. The rest of the strains (2 P. aeruginosa, 2 Acinetobacter baumanii, 1 Sphingomonas paucimobilis) did not give conclusive results. These screening methods are useful, simple and accessible to clinical laboratories. PCR is needed to confirm the presence of metallo-beta-lactamase gene in bacteria and to determine the type of the enzymes.
authors.affiliation: Universitatea de Medicină si Farmacie Gr.T. Popa Iaşi, Facultatea de Medicină, Disciplina de Microbiologie Medicală.
authors.forename: Ana-Irina
authors.initials: AI
authors.lastname: Mereuţă
authors.forename: Antonia
authors.initials: A
authors.lastname: Poiati
authors.forename: Cristina
authors.initials: C
authors.lastname: Tuchiluş
authors.forename: Olivia
authors.initials: O
authors.lastname: Dorneanu
authors.forename: Silvia
authors.initials: S
authors.lastname: Nistor
authors.forename: Brînduşa
authors.initials: B
authors.lastname: Copăcianu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis* / Q000096:biosynthesis; D001769:Blood / Q000382:microbiology; D015780:Carbapenems / Q000494:pharmacology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D008403:Mass Screening; D008826:Microbial Sensitivity Tests; D015337:Multicenter Studies as Topic; D010996:Pleural Effusion / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology*; D012383:Romania; D013492:Suppuration / Q000382:microbiology; D014556:Urine / Q000382:microbiology; D001618:beta-Lactamases / Q000032:analysis* / Q000096:biosynthesis
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C082063:cphA protein, Aeromonas hydrophila