[Serophysiological study on the effects of high-fat diet on proliferation of human lung adenocarcinoma cell line SPCA1].
OBJECTIVE
The present study was designed to assess the effect of the diet high, in fat on proliferative activities of lung adenocarcinoma cell line SPCA1 by means of serophysiological method at the cell level.


METHODS
Female Sprague-Dawley rats were fed with common chow or high-fat diet (lard : common chow = 1 : 9) for 7 weeks after having ascertained the suitable conditions for cell culture in serophysiological study. Then the serum from the rat fed with different diet was obtained and used for culturing human lung adenocarcinoma cell line SPCA1. The cells were harvested and used for analysis in MTT assay, [3H] thymidine incorporation trial and flow cytometric analysis to identify cell proliferative activity, DNA duplication and cell cycle distribution, which were examined to discuss the effects of rat's serum treated with high-fat diet (RSTHFD) on proliferation of cancer cells.


RESULTS
(1) Fifteen percent of inactivated rat serum supplemented in culture medium was suitable condition for culturing human lung adenocarcinoma cell line SPCA1. (2) RSTHFD can accelerate DNA duplication, cell proliferation and cell mitosis.


CONCLUSIONS
(1) Serophysiological methods can be used to study the effects of diet on proliferation of SPCA1 cell line. (2) High-fat diet can stimulate the proliferative activity of SPCA1 cells in the present study.
Wang
Jun-Fang
JF
West China School of Public Health, Sichuan University, Chengdu 610041, China. 2002-annie@163.com
Huang
Cheng-Yu
CY
Xu
Jia-Yu
JY
Xiong
Ying
Y
Zhao
Qiuling
Q
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003470:Culture Media; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum
D003470:Culture Media; D004041:Dietary Fats