Bovine deciduous dentine is more susceptible to osteoclastic resorption than permanent dentine: results of quantitative analyses.
Many clinical reports suggest that deciduous teeth exhibit a greater susceptibility to resorption than permanent ones. To examine the difference between deciduous and permanent dentine in their susceptibility to osteoclastic resorption, osteoclast-like cells (OCLs) were cultured on deciduous and permanent dentine slices. The number, area, depth, and volume of resorption pits were then measured, using image-analyzing systems. We measured the level of degraded collagen (cross-linked N-telopeptide of type I collagen; NTx) in culture medium using an enzyme-linked immunosorbent assay (ELISA). The levels of cathepsin K, matrix metallo proteinase (MMP)-9, and MMP-13 mRNAs in the cells attached to dentine were also analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR). Deciduous dentine slices exhibited a significant (twofold) increase in resorbed area compared with the permanent slices. Three-dimensional analysis revealed that the volume of pits in deciduous dentine differed significantly (fourfold) compared to that in the permanent dentine. The depth of pits also followed the same trend. However, there was no significant difference in the number of pits or osteoclasts on the dentine slices. The NTx level in deciduous media was significantly more than that in permanent media. The mRNA levels also followed the same trend. These results suggest that deciduous dentine is more susceptible to resorption than permanent dentine and signals from the substrate play an important role in physiological resorption.
Varghese
Bobby John
BJ
Developmental Oral Health Science, Department of Orofacial Development and Function, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Tokyo, 113-8549, Japan.
Aoki
Kazuhiro
K
Shimokawa
Hitoyata
H
Ohya
Keiichi
K
Takagi
Yuzo
Y
D000818:Animals; D001862:Bone Resorption / Q000503:physiopathology*; D056657:Cathepsin K; D002403:Cathepsins / Q000096:biosynthesis; D002417:Cattle; D002478:Cells, Cultured; D024042:Collagen Type I / Q000032:analysis / Q000737:chemistry; D017364:Collagenases / Q000096:biosynthesis; D003804:Dentin / Q000473:pathology / Q000503:physiopathology*; D003809:Dentin, Secondary / Q000503:physiopathology*; D008297:Male; D053509:Matrix Metalloproteinase 13; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D051379:Mice; D008853:Microscopy; D010010:Osteoclasts / Q000502:physiology; D010455:Peptides / Q000032:analysis; D014094:Tooth, Deciduous / Q000503:physiopathology*
D024042:Collagen Type I; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide; D002403:Cathepsins; D056657:Cathepsin K; C534132:Ctsk protein, mouse; D017364:Collagenases; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse; D020780:Matrix Metalloproteinase 9