Analysis of ovarian tumors for the presence of human papillomavirus DNA.
OBJECTIVE
The role of human papillomavirus (HPV) infection in ovarian tumorigenesis is uncertain. The objective of this study was to screen a collection of ovarian tumors for the presence of high-risk oncogenic HPV types 16, 18 and 33.


METHODS
Twenty benign and malignant ovarian tumors were obtained from women undergoing pelvic surgery at a regional comprehensive cancer institution in North America. DNA was isolated from the snap-frozen tumors, and commercial polymerase chain reaction (PCR) detection sets were used to analyze the tumor DNA samples for the presence of DNA from HPV types 16, 18 and 33.


RESULTS
The DNA from HPV types 16, 18 and 33 was not detected in any of the ovarian tumors.


CONCLUSIONS
Our findings do not support an association between infection with HPV types 16, 18 and 33 and ovarian neoplasia in this patient population.
Quirk
Jeffrey T
JT
Department of Biology, State University of New York - Jamestown Community College, Jamestown, New York 14702, USA. jtq@velocity.net
Kupinski
John M
JM
DiCioccio
Richard A
RA
D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000601:surgery / Q000821:virology*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral