title: [High-level expression of phenylalanine ammonia-lyase in Lactococcus Lactis via synthesized sequence based on bias codons].
abstract: To construct a safer and more efficient gene engineering Lactococcus Lactis for expressing phenylalaine ammonia lyase (PAL) which will be benefit for PKU therapy, pal cDNA of Parsly and synthesized sequence based on Lactococcus Lactis bias codons were recombined into two Lactococcus Lactis NICE systems. The activities of the expressed PAL were detected, and the effect of Lactococcus Lactis bias codons on the expression of exterior protein was analyzed. The results showed that the expression level of PAL was increased by using Lactococcus Lactis bias codons in both Lactococcus Lactis NICE systems. Through which several safer andmore efficient strains of the gene engineering Lactococcus Lactis were obtained.
authors.affiliation: Beijing Chaoyang Hospital Affiliate of Capital University of Medical Sciences, Beijing 100020, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Gao
authors.forename: Xing-Yuan
authors.initials: XY
authors.lastname: Jia
authors.forename: Chang
authors.initials: C
authors.lastname: Su
authors.forename: Yue-Ping
authors.initials: YP
authors.lastname: Lü
authors.forename: Zhan-Yong
authors.initials: ZY
authors.lastname: Wang
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Fan
authors.forename: Bai
authors.initials: B
authors.lastname: Xiao
authors.forename: Jing-Zhong
authors.initials: JZ
authors.lastname: Liu
mesh_terms: D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000378:metabolism; D014169:Transformation, Bacterial
chemical_list: D003062:Codon; D011994:Recombinant Proteins; D010650:Phenylalanine Ammonia-Lyase