title: Evaluation of a novel Vi conjugate vaccine in a murine model of salmonellosis.
abstract: Immunisation of BALB/c mice with a vaccine containing Vi polysaccharide conjugated to the Klebsiella pneumoniae outer membrane 40 kDa protein (rP40), in combination with Escherichia coli heat-labile toxin adjuvant (LT), elicited anti-Vi IgG antibodies after administration using different routes. Testing of the immune serum in opsonisation assays demonstrated the specific enhancement of Vi-positive bacterial uptake by cultured murine bone marrow derived macrophages. Intra-peritoneal challenge of mice immunised with the Vi-based vaccine elicited a degree of protection against virulent Vi+ Salmonella enterica serovar typhimurium (S. typhimurium). In contrast, Vi vaccination did not confer protection against oral challenge with virulent Vi-positive S. typhimurium or S. dublin.
authors.affiliation: The Wellcome Trust Genome Campus, The Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK. cbh@sanger.ac.uk
authors.forename: Christine
authors.initials: C
authors.lastname: Hale
authors.forename: Frances
authors.initials: F
authors.lastname: Bowe
authors.forename: Derek
authors.initials: D
authors.lastname: Pickard
authors.forename: Simon
authors.initials: S
authors.lastname: Clare
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Haeuw
authors.forename: Ultan
authors.initials: U
authors.lastname: Powers
authors.forename: Nathalie
authors.initials: N
authors.lastname: Menager
authors.forename: Pietro
authors.initials: P
authors.lastname: Mastroeni
authors.forename: Gordon
authors.initials: G
authors.lastname: Dougan
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012480:Salmonella Infections / Q000276:immunology / Q000517:prevention & control*
chemical_list: D001428:Bacterial Vaccines; D011135:Polysaccharides, Bacterial; C054154:capsular polysaccharide, Salmonella