[Inhibitory effects of mizolastine on substance P-induced production of leukotriene B4 and interleukin 5 in mouse skin].
To observe the inhibitory effect of mizolastine on substance P(SP)-induced production of leukotriene B(4) (LTB(4)) and interleukin 5 (IL-5) in mouse skin.



Mice were fed with different doses of mizolastine or other control drugs, 30 min after administration animals were injected intradermally with SP on the back. The treated skin samples were taken and competitive enzyme-link immunoassay (ELISA) method was applied to detect LTB (4) and IL-5 in the skin samples.



The LTB(4) and IL-5 levels in 10 mg/kg mizolastine group were (1.23 +/-0.29)pg/ml and (34.28 +/-11.00)pg/ml, respectively, which were lower than those in saline control group [(5.52+/-1.88)pg/ml and (179.62 +/-46.25)pg/ml respectively] or loratadine group [(3.89+/-1.27)pg/ml and (127.74 +/-43.27)pg/ml respectively]. No significant difference was found between 10 mg/kg mizolastine group and dexamethasone group (P=0.161 and P=0.508).



Mizolastine might inhibit the production of LTB(4) and IL-5 induced by substance P in mouse skin, suggesting that anti-inflammatory effect and the blockade of histamine H1 receptors might be involved in its anti-pruritic mechanisms.
Wang
Yi-na
YN
The First Affiliated Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310003, China.
Fang
Hong
H
Zhou
Zong-li
ZL
Yao
Hang-ping
HP
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D005260:Female; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D015848:Interleukin-5 / Q000096:biosynthesis*; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000378:metabolism*; D013373:Substance P / Q000037:antagonists & inhibitors*
D001562:Benzimidazoles; D006634:Histamine H1 Antagonists; D015848:Interleukin-5; D007975:Leukotriene B4; C076170:mizolastine; D013373:Substance P