Effect of different organotins on DNA of mollusk (Scapharca inaequivalvis) erythrocytes assessed by the comet assay.
The alkaline comet assay, employing a single-cell gel-electrophoresis, is a rapid, simple and sensitive technique for visualizing and measuring DNA damage leading to strand breakage in individual cells. In this study, we report data about the effect of different organotin compounds (MBTC, DBTC and TBTC) on DNA from erythrocytes of the Scapharca inaequivalvis bivalve mollusc. Our results show significant DNA damage after 30 min in vitro incubation with 10microM of organotins. Since TBTC turned out to be the most genotoxic compound, followed by MBTC and DBTC, we exposed the molluscs to 50ppb of TBTC for 11 days. A significant increase of comet parameters was measured in our experimental conditions. The use of the comet test as a high-throughput screening assay to monitor the effect of environmental pollutants on marine organisms has been proposed.
Gabbianelli
R
R
Dipartimento di Biologia Molecolare, Cellulare e Animale, Università degli Studi di Camerino, Via Camerini 2, I-62032 Camerino (MC) Italy. rosita.gabbianelli@unicam.it
Moretti
M
M
Carpenè
E
E
Falcioni
G
G
D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D006434:Hemocytes / Q000187:drug effects* / Q000378:metabolism; D009947:Organotin Compounds / Q000633:toxicity*; D049894:Scapharca / Q000187:drug effects* / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D009947:Organotin Compounds; D014874:Water Pollutants, Chemical