title: [Serological detection of anti HPV 16/18 and its association with pap smear in adolescents and young women].
abstract: OBJECTIVE
  Verify the relation between HPV 16/18 antibodies and the association with cervical cytology findings in adolescents and young women.
  
  
  METHODS
  A cross sectional study with 541 healthy and sexually active women from 15 to 25 years of age was carried on from September to November 2000. At gynecological examination, a cervical sample on liquid-base for cytology-testing and a blood sample for ELISA identification of HPV 16 and 18 antibodies were collected and sent to a reference laboratory in Belgium. Statistical analysis estimated the prevalence and prevalence ratio with a 95% confidence interval.
  
  
  RESULTS
  Of these young women, 150 (27.7%) were seropositive: 79 (14.6%) to HPV 16 antibody; 35 (6.4%) to HPV 18 antibody and 36 (6.6%) to both HPV 16/18 antibodies. Cytological abnormalities were detected in 107 cases (20.5%): 63 presented with atypical squamous cells of undetermined significance (ASCUS), 41 were suggestive of low grade squamous intra-epithelial lesion (LGSIL) and 3 of high grade squamous intra-epithelial lesion (HGSIL). Prevalence of abnormal cytology with a positive serology was only 1.75 times that of prevalence of abnormal cytology with a negative serology. Detection of abnormal cytology was not correlated with positive serology.
  
  
  CONCLUSIONS
  Results of this study indicate a high prevalence of HPV 16 and 18 in young healthy women which showed no relation to the abnormal findings of cytology smears.
authors.affiliation: Faculdade de Saúde Pública, Universidade de São Paulo, SP.
authors.forename: Cristina Helena
authors.initials: CH
authors.lastname: Rama
authors.forename: Cecilia Maria
authors.initials: CM
authors.lastname: Roteli-Martins
authors.forename: Sophie Françoise Mauricette
authors.initials: SF
authors.lastname: Derchain
authors.forename: Eliane Z de
authors.initials: EZ
authors.lastname: Oliveira
authors.forename: José Mendes
authors.initials: JM
authors.lastname: Aldrighi
authors.forename: Corintio
authors.initials: C
authors.lastname: Mariani Neto
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D001938:Brazil / Q000453:epidemiology; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D052162:Human papillomavirus 16 / Q000276:immunology*; D052161:Human papillomavirus 18 / Q000276:immunology*; D006801:Humans; D065006:Papanicolaou Test; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000453:epidemiology; D015995:Prevalence; D016036:Seroepidemiologic Studies; D014626:Vaginal Smears
chemical_list: D000914:Antibodies, Viral