Molecular diagnosis of avian nephritis: preliminary report.
Kidney samples from chickens diagnosed with acute nephritis and gout were subjected to histological and electron microscopic examination. The investigations revealed cytoplasmic inclusion bodies in the tubular epithelial cells containing round virions of about 30 nm in diameter. Since avian nephritis virus (ANV) is known as a potential causative agent of the so-called baby chick nephropathy, an RT-PCR assay was developed for the molecular detection of ANV-specific nucleic acid in the specimen. The specificity of the assay was confirmed by direct sequencing of the amplicon obtained in the reaction. The nucleotide sequence of the PCR product showed 92% identity with the reference ANV sequence deposited in the GenBank database. After having been validated on some other suspicious cases of avian nephritis, the PCR method described in this study can be a potential tool for routine diagnostic examination of samples submitted from cases of gout and nephropathy in chickens.
Mándoki
Mira
M
Department of Pathology and Forensic Veterinary Medicine, Veterinary Faculty, Szent István University, H-1400 Budapest, P.O. Box 2, Hungary. Mandoki.Mira@aotk.szie.hu
Dobos-Kovács
M
M
Bakonyi
T
T
Rusvai
M
M
D000208:Acute Disease; D000818:Animals; D019200:Astroviridae / Q000235:genetics / Q000302:isolation & purification*; D019350:Astroviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D001483:Base Sequence; D002645:Chickens; D017931:DNA Primers; D006073:Gout / Q000175:diagnosis / Q000662:veterinary*; D008969:Molecular Sequence Data; D009393:Nephritis / Q000175:diagnosis / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology; D011237:Predictive Value of Tests; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
D017931:DNA Primers; D012367:RNA, Viral