Luminescent metal-ligand complexes as probes of macromolecular interactions and biopolymer dynamics.
The knowledge of microsecond dynamics is important for an understanding of the mechanism and function of biological systems. Fluorescent techniques are well established in biophysical studies, but their applicability to probe microsecond timescale processes is limited. Luminescent metal-ligand complexes (MLCs) have created interest mainly due to their unique luminescent properties, such as the exceptionally long decay times and large fundamental anisotropy values, allowing examination of microsecond dynamics by fluorescence methods. MLC properties also greatly simplify instrumentation requirements and enable the use of light emitting diode excitation for time-resolved measurements. Recent literature illustrates how MLC labels take full advantage of well developed fluorescence techniques and how those methods can be extended to timescales not easily accessible with nanosecond probes. MLCs are now commercially available as reactive labels which give researchers access to methods that previously required more complex approaches. The present paper gives an overview of the applications of MLC probes to studies of molecular dynamics and interactions of proteins, membranes and nucleic acids.
Piszczek
Grzegorz
G
Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA. grzegorz_piszczek@nih.gov
D001704:Biopolymers / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D007700:Kinetics; D008024:Ligands; D008163:Luminescent Measurements / Q000379:methods*; D046911:Macromolecular Substances / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D015336:Molecular Probe Techniques
D001704:Biopolymers; D008024:Ligands; D046911:Macromolecular Substances; D008670:Metals