title: The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
abstract: Dendritic cells (DCs) that capture apoptotic cells (ACs) in the steady state mediate peripheral tolerance to self-antigens. ACs are recognized by an array of receptors on DCs, the redundancy of which is not completely defined. We made use of an AC surrogate system to address the individual roles of the alphavbeta5 and complement receptors (CRs) in the phagocytosis and induction of immunity. CR3 and CR4, while substantially less efficient than alphavbeta5 in internalizing ACs, initiate signals that render DCs tolerogenic. Responding T cells show impaired proliferation and IFNgamma production and subsequently die by apoptosis. While tolerogenic DCs are not induced via alphavbeta5, coligation of CR3 and alphavbeta5 maintains the DC's tolerogenic profile. This immunomodulatory role, however, is countered by a significant inflammatory stimulus such as bacterial infection. Overall, our data suggest that under steady-state conditions, signaling via CRs predominates to render DCs tolerogenic.
authors.affiliation: New York University School of Medicine, 550 First Ave, MSB 507, New York, NY 10016, USA.
authors.forename: Mojca
authors.initials: M
authors.lastname: Skoberne
authors.forename: Selin
authors.initials: S
authors.lastname: Somersan
authors.forename: Wanda
authors.initials: W
authors.lastname: Almodovar
authors.forename: Tuan
authors.initials: T
authors.lastname: Truong
authors.forename: Kseniya
authors.initials: K
authors.lastname: Petrova
authors.forename: Peter M
authors.initials: PM
authors.lastname: Henson
authors.forename: Nina
authors.initials: N
authors.lastname: Bhardwaj
mesh_terms: D017209:Apoptosis / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000276:immunology / Q000502:physiology; D016023:Integrins / Q000276:immunology / Q000502:physiology*; D016177:Macrophage-1 Antigen / Q000276:immunology / Q000502:physiology*; D010587:Phagocytosis / Q000276:immunology; D019030:Receptors, Vitronectin / Q000276:immunology / Q000502:physiology*; D017634:Self Tolerance / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D016167:Integrin alphaXbeta2; D016023:Integrins; D016177:Macrophage-1 Antigen; D019030:Receptors, Vitronectin; C064660:integrin alphaVbeta5