title: Modeling a whole organ using proteomics: the avian bursa of Fabricius.
abstract: While advances in proteomics have improved proteome coverage and enhanced biological modeling, modeling function in multicellular organisms requires understanding how cells interact. Here we used the chicken bursa of Fabricius, a common experimental system for B cell function, to model organ function from proteomics data. The bursa has two major functional cell types: B cells and the supporting stromal cells. We used differential detergent fractionation-multidimensional protein identification technology (DDF-MudPIT) to identify 5198 proteins from all cellular compartments. Of these, 1753 were B cell specific, 1972 were stroma specific and 1473 were shared between the two. By modeling programmed cell death (PCD), cell differentiation and proliferation, and transcriptional activation, we have improved functional annotation of chicken proteins and placed chicken-specific death receptors into the PCD process using phylogenetics. We have identified 114 transcription factors (TFs); 42 of the bursal B cell TFs have not been reported before in any B cells. We have also improved the structural annotation of a newly sequenced genome by confirming the in vivo expression of 4006 "predicted", and 6623 ab initio, ORFs. Finally, we have developed a novel method for facilitating structural annotation, "expressed peptide sequence tags" (ePSTs) and demonstrate its utility by identifying 521 potential novel proteins from the chicken "unassigned chromosome".
authors.affiliation: Department of Basic Science, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762-6100, USA. fmccarthy@cvm.msstate.edu
authors.forename: Fiona M
authors.initials: FM
authors.lastname: McCarthy
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Cooksey
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Susan M
authors.initials: SM
authors.lastname: Bridges
authors.forename: G Todd
authors.initials: GT
authors.lastname: Pharr
authors.forename: Shane C
authors.initials: SC
authors.lastname: Burgess
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002060:Bursa of Fabricius; D002478:Cells, Cultured; D002645:Chickens; D030541:Databases, Genetic; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D010802:Phylogeny; D040901:Proteomics; D016415:Sequence Alignment