[PLAC1/CP1 gene expression and autologous humoral immunity in gastric cancer patients].
OBJECTIVE
To investigate PLAC1/CP1 as a potential candidate gene in gastric cancer therapy.


METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the expression of PLAC1/CP1 mRNA in gastric cancer tissues and paired adjacent non-cancerous tissues resected from 28 patients with gastric cancer. Specific antibodies against PLAC1/CP1 were also detected by ELISA method in gastric cancer patients.


RESULTS
Fourteen (50%,14/28) out of the 28 gastric cancer samples were PLAC1/CP1 mRNA positive. Of the 28 serum samples tested,eight (29%,8/28) displayed positive seroreactivity against PLAC1/CP1 antigen, accounting for 57% (8/14) of PLAC1/CP1 mRNA positive samples.


CONCLUSIONS
PLAC1/CP1 mRNA was expressed in high frequency and induced spontaneous antibody responses in gastric cancer patients. PLAC1/CP1 may be a valuable candidate antigen in gastric cancer immunotherapy.
Chen
Jing
J
Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100083, China.
Pang
Xue-wen
XW
Liu
Fang-fang
FF
Dong
Xue-yuan
XY
Wang
Hong-cheng
HC
Wang
Shan
S
Zhang
Yu
Y
Chen
Wei-feng
WF
D000912:Antibodies, Neoplasm / Q000276:immunology; D005260:Female; D015870:Gene Expression; D006801:Humans; D056724:Immunity, Humoral; D008297:Male; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*
D000912:Antibodies, Neoplasm; C415583:PLAC1 protein, human; D011257:Pregnancy Proteins