title: Stimulatory effects of Antidesma madagascariense on D-glucose, L-tyrosine, fluid and electrolyte transport across rat everted intestine, comparable to insulin action in vitro.
abstract: Medicinal plants are believed to be an important source of potential therapeutic agents. This study investigates the effects of Antidesma madagascariense (AM) extract on the transport of D-glucose, L-tyrosine, fluid and electrolytes (Na+ and K+) across rat everted intestinal sacs. These sacs were mounted in an organ bath containing Krebs-Henseleit bicarbonate (KHB) buffer. Experimental findings showed that incubation with graded aqueous AM extracts above 0.375 mg/mL significantly (P < 0.05) stimulated the mucosal disappearance and serosal appearance of glucose and fluid. The concentration of glucose accumulated in the intestinal tissues also increased significantly (P < 0.05) compared to that found in the controls. Transport of the amino acid L-tyrosine was not significantly enhanced (P > 0.05) when incubated with increasing concentrations of AM extract. Effects on electrolyte (K+ and Na+) transport were assessed. Na+ uptake and transport was significantly enhanced (P < 0.05) when incubated with 0.75 mg/mL AM extract; however, K+ transport was not significantly enhanced (P > 0.05). For comparison, insulin (1 and 2 units/mL) was incubated in the mucosal solution. Aqueous AM extract produced similar stimulatory effects on the transport of glucose, fluid and Na+ as were found with insulin. It is hypothesised that bioactive phytochemicals such as flavonoids, alkaloids, leucoanthocyanins, phenols and saponins from AM leaf extract might interfere with the Na+/glucose carrier, thereby enhancing the transport of glucose, Na+ and fluid across rat everted intestinal sacs. Thus, AM may represent a possible alternative dietary supplement for the treatment of type 2 diabetes.
authors.affiliation: Department of Health Sciences, Faculty of Science, University of Mauritius, Reduit, Mauritius.
authors.forename: M F
authors.initials: MF
authors.lastname: Mahomoodally
authors.forename: A Gurib
authors.initials: AG
authors.lastname: Fakim
authors.forename: A H
authors.initials: AH
authors.lastname: Subratty
mesh_terms: D000818:Animals; D003920:Diabetes Mellitus / Q000628:therapy; D004573:Electrolytes / Q000378:metabolism; D028481:Euphorbia; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D008297:Male; D008436:Mauritius; D009924:Organ Culture Techniques; D009995:Osmosis; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014443:Tyrosine / Q000378:metabolism
chemical_list: D004573:Electrolytes; D007328:Insulin; D010936:Plant Extracts; D014443:Tyrosine; D005947:Glucose