Shedding UV light on the phase problem.
In this issue of Structure, Nanao and Ravelli (2006) describe the use of UV-induced radiation damage (UV-RIP) to solve the phase problem for proteins, employing single-wavelength X-ray radiation, without the need for derivatization. This should also permit data collection for many proteins on home sources, without travel to a synchrotron.
Sussman
Joel L
JL
Silman
Israel
I
D001671:Biochemistry / Q000379:methods*; D055592:Biophysical Phenomena; D001703:Biophysics; D018360:Crystallography, X-Ray / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry; D012542:Scattering, Radiation; D017356:Synchrotrons; D014466:Ultraviolet Rays; D014961:X-Ray Diffraction; D014965:X-Rays
D011506:Proteins