title: Characterization of volatile constituents of Haplopappus greenei and studies on the antifungal activity against phytopathogens.
abstract: Essential oil of Haplopappus greenei A. Gray was obtained by hydrodistillation of aerial parts, which were subsequently analyzed by gas chromatography and gas chromatography-mass spectrometry. Major components were identified as carvacrol (8.7%), beta-pinene (7.6%), trans-pinocarveol (6.2%), and caryophyllene oxide (5.8%), respectively. In total, 104 components representing 84.9% of the investigated essential oil were characterized. Furthermore, the essential oil was evaluated for antimalarial, antimicrobial, and antifungal activities. However, only antifungal activity was observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, Colletotrichum fragariae, and Colletotrichum gloeosporioides using the direct overlay bioautography assay. Major essential oil components were also evaluated for antifungal activity; the carvacrol standard demonstrated nonselective activity against the three Colletotrichum species and the other compounds were inactive.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey. bdemirca@anadolu.edu.tr
authors.forename: Betul
authors.initials: B
authors.lastname: Demirci
authors.forename: Kemal Husnu Can
authors.initials: KH
authors.lastname: Baser
authors.forename: Nurhayat
authors.initials: N
authors.lastname: Tabanca
authors.forename: David E
authors.initials: DE
authors.lastname: Wedge
mesh_terms: D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds / Q000032:analysis; D002849:Chromatography, Gas; D020231:Colletotrichum / Q000187:drug effects*; D000081007:Cymenes; D005659:Fungicides, Industrial / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D031206:Haplopappus / Q000737:chemistry*; D039821:Monoterpenes / Q000032:analysis / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D010944:Plants / Q000382:microbiology*
chemical_list: D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D000081007:Cymenes; D005659:Fungicides, Industrial; D039821:Monoterpenes; D009822:Oils, Volatile; C010789:beta-pinene; C073316:carvacrol