title: Extended resolution wide-field optical imaging: objective-launched standing-wave total internal reflection fluorescence microscopy.
abstract: Standing-wave total-internal-reflection fluorescence (SW-TIRF) microscopy uses a super-diffraction-limited standing evanescent wave to extract the high-spatial-frequency content of an object through a diffraction-limited optical imaging system. The effective point-spread function is better than a quarter of the emission wavelength. With a 1.45 numerical aperture objective and 532 nm excitation wavelength, a Rayleigh resolution of approximately 100 nm can be achieved, which is better than twice the resolution of conventional TIRF microscopy. This first experimental realization of SW-TIRF in an objective-launched geometry demonstrates the potential for extended resolution imaging at high speed by using wide-field microscopy.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA. ogong50@mit.edu
authors.forename: Euiheon
authors.initials: E
authors.lastname: Chung
authors.forename: Daekeun
authors.initials: D
authors.lastname: Kim
authors.forename: Peter T C
authors.initials: PT
authors.lastname: So
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D012031:Refractometry / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity