title: Identification of Aeromonas culicicola by 16S rDNA RFLP.
abstract: Studies were conducted on the improvement of A. culicicola identification. This species is phenotypically very similar to A. veronii biotype sobria, A. sobria, and A. allosaccharophila. The sequences of 16S rDNA of A. culicicola isolates show the highest similarity with A. jandaei, A. veronii, and A. caviae. Digestion of 16S rDNA PCR product with AluI and MboI restriction endonucleases allowed discriminating A. culicicola from all other Aeromonas species with the exception of A. jandaei. Additional digestion of 16S rDNA PCR product with BceAI showed a possibility of distinguishing A. jandaei from A. culicicola.
authors.affiliation: Department of Microbiology, Institute of Experimental Biology, A Mickiewicz University, Poznań, Poland. akazn@amu.edu.pl
authors.forename: Adam
authors.initials: A
authors.lastname: Kaznowski
authors.forename: Edyta
authors.initials: E
authors.lastname: Konecka
mesh_terms: D000333:Aeromonas / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005798:Genes, Bacterial; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004262:DNA Restriction Enzymes