title: New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum.
abstract: Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of developmental stages (cercariae, hepatic schistosomula, female and male adults, eggs, and miracidia) and tissues at the host-parasite interface (eggshell and tegument) by interrogating the protein database deduced from the contigs. Comparative analysis of these transcriptomic and proteomic data, the latter including 3,260 proteins with putative identities, revealed differential expression of genes among the various developmental stages and sexes of S. japonicum and localization of putative secretory and membrane antigens, enzymes, and other gene products on the adult tegument and eggshell, many of which displayed genetic polymorphisms. Numerous S. japonicum genes exhibited high levels of identity with those of their mammalian hosts, whereas many others appeared to be conserved only across the genus Schistosoma or Phylum Platyhelminthes. These findings are expected to provide new insights into the pathophysiology of schistosomiasis and for the development of improved interventions for disease control and will facilitate a more fundamental understanding of schistosome biology, evolution, and the host-parasite interplay.
authors.affiliation: Chinese National Human Genome Center, Shanghai, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Jiong
authors.initials: J
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Sheng-Yue
authors.initials: SY
authors.lastname: Wang
authors.forename: Shu-Jian
authors.initials: SJ
authors.lastname: Cui
authors.forename: Ming
authors.initials: M
authors.lastname: Chi
authors.forename: Qing
authors.initials: Q
authors.lastname: Yan
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Wang
authors.forename: Huai-Dong
authors.initials: HD
authors.lastname: Song
authors.forename: Xue-Nian
authors.initials: XN
authors.lastname: Xu
authors.forename: Ju-Jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Xiang-Lin
authors.initials: XL
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhi-Qin
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Chun-Liang
authors.initials: CL
authors.lastname: Xue
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Brindley
authors.forename: Donald P
authors.initials: DP
authors.lastname: McManus
authors.forename: Peng-Yuan
authors.initials: PY
authors.lastname: Yang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Feng
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Ze-Guang
authors.initials: ZG
authors.lastname: Han
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D017238:Genes, Helminth; D023281:Genomics; D006790:Host-Parasite Interactions / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010273:Parasitic Diseases, Animal / Q000235:genetics / Q000469:parasitology*; D040901:Proteomics; D011817:Rabbits / Q000469:parasitology; D012376:Rodent Diseases / Q000469:parasitology; D012549:Schistosoma japonicum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012554:Schistosomiasis japonica / Q000235:genetics / Q000378:metabolism / Q000469:parasitology*; D012908:Snails / Q000469:parasitology