Does Botox effect neural tube development in early chick embryos?
Botulinum toxin (BTX) is a potent neurotoxin produced by Clostridium botulinum and has wide usage in different areas. The current study aimed to analyze the effects of C. botulinum toxin on the central nerve system in chick embryos. Forty fertile Hubbard Broil eggs, all at Stage 8 of development, were divided into four equal groups: Group 1 embryos (n=10), the control group, were explanted and grown for 18 h in a nutrient medium (thin albumin). Group 2 embryos (n=10) were grown in medium containing 5 U BTX, Group 3 embryos (n=10) in a medium containing 10 U BTX and Group 4 embryos (n=10) in medium containing 20 U BTX. After the incubation period, 80% of Group 1 and 2 embryos and 90% of Group 3 and 4 embryos had intact neural tubes (P>0.05). The results of this study suggest that BTX had no additional effect on neural tube development in early chick embryos.
Eser
Olcay
O
Department of Neurosurgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
Yaman
Mehmet
M
Cosar
Emine
E
Konak
Abdullah
A
Cosar
Murat
M
Sahin
Onder
O
Güney
Onder
O
D000818:Animals; D019274:Botulinum Toxins, Type A / Q000494:pharmacology*; D002490:Central Nervous System / Q000196:embryology* / Q000473:pathology; D002642:Chick Embryo; D003014:Clostridium botulinum; D047108:Embryonic Development / Q000187:drug effects*; D009498:Neurotoxins / Q000494:pharmacology*
D009498:Neurotoxins; D019274:Botulinum Toxins, Type A