title: Examination of the biological effects of high anionic peroxidase production in tobacco plants grown under field conditions. I. Insect pest damage.
abstract: At least 25 wild type and high peroxidase tobacco Nicotiana tabacum L. plants were examined semiweekly over several weeks for pest insect distribution and damage in a 2 year field study. Incidence and/or severity of naturally occurring caterpillar damage (dingy cutworm (Feltia ducens Walker), black cutworm (Agrotis ipsilon (Hufnagel), tobacco hornworm (Manduca sexta L.), and false tobacco budworm (= corn earworm Helicoverpa zea (Boddie)) was significantly reduced at several sample dates for high peroxidase vs. wild type plants. These results parallel those of prior laboratory studies with caterpillars. The number of adult whiteflies (Trialeurodes vaporariorum (Westwood) per plant was significantly reduced on high peroxidase compared to wild type plants on most sample dates in both years. The number of plants with leaves containing >100 aphids (primarily Myzus persicae Sulzer) per leaf on high peroxidase plants was significantly lower that on wild type plants after an equivalent invasion period in both years. A significantly higher proportion of aphids were found dead on leaf five of high peroxidase compared to wild type plants at most sample dates in both years. These results indicate that high peroxidase plants have resistance to a wide range of insects, implicating this enzyme as a broad range resistance mechanism.
authors.affiliation: Crop Bioprotection Research Unit, National Center For Agricultural Utilization Research, USDA, Agricultural Research Service, Peoria, IL 61604, USA. dowdpf@ncaur.usda.gov
authors.forename: Patrick F
authors.initials: PF
authors.lastname: Dowd
authors.forename: L Mark
authors.initials: LM
authors.lastname: Lagrimini
mesh_terms: D000818:Animals; D001042:Aphids; D007300:Insect Control; D007814:Larva; D009036:Moths; D010544:Peroxidases / Q000235:genetics*; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*
chemical_list: D010544:Peroxidases; C120494:anionic peroxidase