[Screening of aryl hydrocarbon receptor gene specific RNA interference fragment by quantitative competitive RT-PCR].
OBJECTIVE
To evaluate and screen the specific RNAi fragments which can effectively inhibit Aryl hydrocarbon receptor(AHR) gene mRNA expression in human bronchial epithelial cell line (16HBE).


METHODS
AHR mRNA of 16HBE cells transfected 4 different AHR gene interfere sites were determined quantitatively with the quantitative competitive RT-PCR by using self-prepared internal standard as competitive templates, and the RNA interfere effect wasevaluated.


RESULTS
AHR mRNA average expression per 40ng total RNA of 16HBE cells transfected 4 different AHR gene interfere fragments were 5.65fg, 14.78fg, 3.14fg and 0.68fg respectively, the average rates of inhibition were 61.6%, -0.5%, 78.6% and 95.4% respectively.


CONCLUSIONS
AHR gene specific effective RNA interfere sequence ware screened by quantitative competitive RT-PCR which could accurately quantify gene mRNA level, and offered condition for studying the gene function of AHR.
Lai
Yan-Dong
YD
Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou 510182, China.
Li
Xiu-Ying
XY
Bin
Xiao-Nong
XN
Lü
Jia-Chun
JC
D001667:Binding, Competitive; D001980:Bronchi / Q000166:cytology*; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018336:Receptors, Aryl Hydrocarbon