title: [The detection of dysplastic lesions of the gallbladder mucosa in polypoid cholelithiasis].
abstract: OBJECTIVE
  To investigate the hyperplastic and/or dysplastic lesions in the gallbladder mucosa.
  
  
  METHODS
  We used the immunohistochemical Ardeleanu-Hsu-Bussolati and Gugliotta methods, with avidin and peroxide biotin, adapted in the laboratory of University Hospital "St. Spiridon" Iaşi. There were studied ten cases of polypoid cholelithiasis, with the following markers: Ki67, PCNA, and p53.
  
  
  RESULTS
  The immunohistochemical methods using Ki67, PCNA and p53 protein allowed the evaluation of the abnormal proliferation abilities characteristic for some hyperplastic or dysplastic lesions, found in our cases of cholelithiasis.
  
  
  CONCLUSIONS
  The utilization of these markers permitted us to establish a good correlation between the high activity of Ki67 and the morphological aggression indicators in hyperplastic and dysplastic gallbladder epithelium. There is also a correlation between the percentage of the cells marked with anti-PCNA antibody and the existence of p53 protein in these lesions.
authors.affiliation: Universitatea de Medicină şi Farmacie Gr T Popa Iaşi, Facultatea de Medicină, Disciplina de Histologie.
authors.forename: Maria Elisabeta
authors.initials: ME
authors.lastname: Bădescu
authors.forename: Manuela
authors.initials: M
authors.lastname: Stancu
authors.forename: G I
authors.initials: GI
authors.lastname: Pandele
authors.forename: Laurette
authors.initials: L
authors.lastname: Cozma
authors.forename: Gioconda
authors.initials: G
authors.lastname: Dobrescu
authors.forename: Cornelia
authors.initials: C
authors.lastname: Amălinei
authors.forename: Adriana
authors.initials: A
authors.lastname: Grigoraş
authors.forename: Adriana
authors.initials: A
authors.lastname: Bădescu
authors.forename: Dana
authors.initials: D
authors.lastname: Baran
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D002769:Cholelithiasis / Q000737:chemistry / Q000150:complications / Q000473:pathology* / Q000601:surgery; D005704:Gallbladder / Q000473:pathology; D005706:Gallbladder Neoplasms / Q000737:chemistry / Q000150:complications / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D009092:Mucous Membrane / Q000473:pathology; D011127:Polyps / Q000737:chemistry / Q000150:complications / Q000473:pathology* / Q000601:surgery; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53