[Sodium transport and its regulation of acutely isolated alveolar type II cells].
OBJECTIVE
To observe the sodium current of acutely isolated alveolar type II cells and its regulation.


METHODS
After isolation the AT II cells, the whole cell sodium current were recorded in 5 hours with patch-clamp in whole-cell mode, and the effect of amiloride and terbutaline on the current was investigated.


RESULTS
The amiloride-sensitive current of AT II cells were recorded, which could be obviously stimulated by terbutaline.


CONCLUSIONS
Acutely isolated AT II cells can be valuable for studying electrophysiological characteristics of sodium channels even under pathological conditions.
Shen
Hai-yan
HY
Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Li
Tao-ping
TP
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D002469:Cell Separation; D018408:Patch-Clamp Techniques; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*; D026941:Sodium Channel Blockers / Q000494:pharmacology; D015222:Sodium Channels / Q000378:metabolism* / Q000502:physiology; D013726:Terbutaline / Q000494:pharmacology
D026941:Sodium Channel Blockers; D015222:Sodium Channels; D000584:Amiloride; D012964:Sodium; D013726:Terbutaline