Top-down proteomic analysis of the soluble sub-proteome of the obligate thermophile, Geobacillus thermoleovorans T80: insights into its cellular processes.
We report the first analysis of the soluble sub-proteome of the obligate thermophile, Geobacillus thermoleovorans T80, utilizing a robust multidimensional protein identification protocol. A total of 1,336 proteins were initially identified utilizing automated MS/MS identification software. Intensive manual curation resulted in a final list containing a total of 294 unique proteins. Physiochemical characterization and functional classification of the soluble sub-proteome was carried out. The strategy has allowed us to gain an insight into the cellular processes of this obligate thermophile, identifying a variety of proteins known to play a role in stress response. Included within these were a number of sigma factors such as sigma(A) that initiate transcription of the heat shock operons controlled by the HrcA-CIRCE complex within gram positive bacteria. In addition, it has enabled us to assign a degree of functionality to 29 out of 36 gene products detected in this study that were hitherto described as being only hypothetical conserved proteins.
Graham
Robert Leslie James
RL
School of Biomedical Sciences, University of Ulster, Coleraine, County Londonderry, BT52 1SA, United Kingdom. rl.graham@ulster.ac.uk
Pollock
Catherine E
CE
Ternan
Nigel G
NG
McMullan
Geoff
G
D001406:Bacillaceae / Q000737:chemistry* / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry* / Q000502:physiology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D030562:Databases, Protein; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012995:Solubility; D014357:Trypsin / Q000494:pharmacology
D001426:Bacterial Proteins; D020543:Proteome; D014357:Trypsin