Study of the antifungal ability of Bacillus subtilis strain PY-1 in vitro and identification of its antifungal substance (iturin A).
A Bacillus strain, denoted as PY-1, was isolated from the vascular bundle of cotton. Biochemical, physiological and 16S rDNA sequence analysis proved that it should belong to Bacillus subtilis. The PY-1 strain showed strong ability against many common plant fungal pathogens in vitro. The antibiotics produced by this strain were stable in neutral and basic conditions, and not sensitive to high temperature. From the culture broth of PY-1 strain, five antifungal compounds were isolated by acidic precipitation, methanol extraction, gel filtration and reverse-phase HPLC. Advanced identification was performed by mass spectrometry and nuclear magnetic resonance spectroscopy. These five antifungal compounds were proved to be the isomers of iturin A: A2, A3, A4, A6 and A7. In fast atom bombardment mass spectrometry/mass spectrometry collision-induced dissociation spectra, fragmentation ions from two prior linear acylium ions were observed, and the prior ion, Tyr-Asn-Gln-Pro-Asn-Ser-betaAA-Asn-CO+, was first reported.
Gong
Meng
M
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610064, China.
Wang
Jiang-Dong
JD
Zhang
Jing
J
Yang
Hao
H
Lu
Xiao-Feng
XF
Pei
Yan
Y
Cheng
Jing-Qiu
JQ
D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000737:chemistry / Q000145:classification; D004338:Drug Combinations; D005670:Fusarium / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D009821:Oils; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D010456:Peptides, Cyclic; D010636:Phenols; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
D000935:Antifungal Agents; D004338:Drug Combinations; D009821:Oils; C027222:P & S Liquid; D010455:Peptides; D010456:Peptides, Cyclic; D010636:Phenols; C013579:iturin A