Comparative analysis of the S-intergenic region in class-II S haplotypes of self-incompatible Brassica rapa (syn. campestris).
In the Brassica self-incompatibility (SI) system, a pollen determinant, SP11, is involved in dominance/recessive relationships in pollen SI phenotypes. In order to gain some insights into the genomic structure around the SP11 and the mechanisms that give dominance/recessive relationships, we characterized the genomic region containing SP11 and SRK genes in three pollen recessive class-II S haplotypes. The direction of transcription of S genes was completely conserved among class-II S haplotypes. However, the region between SP11 and SRK (S-intergenic region) was highly polymorphic without short repetitive sequences. In addition, we found a sequence similarity between the short repetitive sequence and 5'-upstream region of SP11. This sequence similarity was found to be potentially related to the expression of dominance relationships through the change of chromatin structure.
Kakizaki
Tomohiro
T
Faculty of Agriculture, Iwate University, Morioka, Japan.
Takada
Yoshinobu
Y
Fujioka
Tomoaki
T
Suzuki
Go
G
Satta
Yoko
Y
Shiba
Hiroshi
H
Isogai
Akira
A
Takayama
Seiji
S
Watanabe
Masao
M
D001483:Base Sequence; D029741:Brassica rapa / Q000235:genetics*; D021901:DNA, Intergenic; D018745:Genome, Plant; D006023:Glycoproteins / Q000235:genetics*; D006239:Haplotypes; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
D021901:DNA, Intergenic; D006023:Glycoproteins; D010940:Plant Proteins; C064156:self-incompatibility locus specific glycoprotein, Brassica