title: Vastus lateralis oxygenation during prolonged cycling in healthy males.
abstract: This study examined the relationship between acute cardiorespiratory and muscle oxygenation and blood volume changes during prolonged exercise. Eight healthy male volunteers (mean maximum oxygen uptake VO(2max) = 41.6 +/- 2.4 mL/kg/min) performed 60 min submaximal cycling at 50% VO(2max). Oxygen uptake VO(2) was measured by indirect spirometry, cardiac output (CO) was estimated using a Portapre, and right vastus lateralis oxyhemoglobin/ myoglobin (oxyHb/Mb), deoxyhemoglobin/myoglobin (deoxyHb/Mb), and total hemoglobin/myoglobin (total Hb/Mb) were recorded using near-infrared spectroscopy (NIRS). After 40 min of exercise, there was a significant increase in VO(2) due to a significantly higher arteriovenous oxygen difference ((a - v)O(2diff). After 30 min of exercise CO remained unchanged, but there was a significant decrease in stroke volume and a proportionate increase in heart rate, thus indicating the occurrence of cardiovascular drift. During the first few minutes of exercise, there was a decline in oxyHb/Mb and total Hb/Mb, whereas deoxyHb/Mb remained unchanged. Thereafter, oxyHb/Mb and total Hb/Mb increased systematically until the termination of exercise while deoxyHb/Mb declined. After 40 min of exercise, these changes were significantly different from the baseline values. There were no significant correlations between the changes in the NIRS variables and systemic VO(2) or mixed (a - v)O(2)diff during exercise. These results suggest that factors other than localized changes in muscle oxygenation and blood volume account for the increased VO(2) during prolonged submaximal exercise.
authors.affiliation: Division of Physical Therapy and Occupational Therapy Sciences, Graduate School of Health Sciences, Hiroshima University, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Kawaguchi
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Kiyokazu
authors.initials: K
authors.lastname: Sekikawa
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Tabusadani
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Inamizu
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Onari
authors.forename: Yagesh
authors.initials: Y
authors.lastname: Bhambhani
mesh_terms: D000328:Adult; D001642:Bicycling / Q000502:physiology*; D006801:Humans; D007866:Leg; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D013997:Time Factors
chemical_list: D010100:Oxygen