title: PslD is a secreted protein required for biofilm formation by Pseudomonas aeruginosa.
abstract: The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region restored the wild-type phenotype. A C-terminal, hexahistidine tag fusion enabled the identification of PslD. LacZ and PhoA translational fusions with PslD indicated that PslD is a secreted protein required for biofilm formation, presumably via its role in exopolysaccharide export.
authors.affiliation: Institute of Molecular Sciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.
authors.forename: Andrea
authors.initials: A
authors.lastname: Campisano
authors.forename: Christine
authors.initials: C
authors.lastname: Schroeder
authors.forename: Mirle
authors.initials: M
authors.lastname: Schemionek
authors.forename: Joerg
authors.initials: J
authors.lastname: Overhage
authors.forename: Bernd H A
authors.initials: BH
authors.lastname: Rehm
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial; D008856:Microscopy, Fluorescence; D009154:Mutation; D019897:Periplasm / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins