title: [Expression of peroxisome proliferator-activated receptors Y changes with age in vascular smooth muscle cells of spontaneously hypertensive rat].
abstract: OBJECTIVE
  In order to elucidate the relationship between PPAR-gamma and the development of hypertension, we detected the expression of peroxisome proliferator-activated receptors gamma (PPAR-gamma) in the vascular smooth muscle cells (VSMCs) and the VSMC proliferation of spontaneously hypertensive rats (SHR) at different ages.
  
  
  METHODS
  The expression of PPAR-gamma in the intact vascular tissues of rats at different ages were detected by immunohistochemistry. Aortic VSMCs were cultured. PPAR-gamma mRNA in primary and low-passage cultured VSMCs of various ages were determined by RT-PCR, and proteins were evaluated by immunohistochemistry and age matched Western Blot. Age matched Wistar-Kyoto rats (WKY) were used as control.
  
  
  RESULTS
  This experiment demonstrated that the expression of PPAR-gamma increased with age in the intact aorta tissues of SHR. The expression of PPAR-gamma did not continuously increase in 24w-old SHR when compared with that in 16w-old SHR. No difference between 4w-, 24w-old SHR and age matched WKY was observed, but the expression of PPAR-gamma was greater in 8w- and 16w old SHR than in age matched WKY. In primary and low-passage cultured VSMCs, the expression of PPAR-gamma mRNA and protein increased with age both in SHR VSMCs and WKY VSMCs of 4w, 8w and 16w old, and no difference between 4w- and 24w-old SHR and WKY was noted, but the expression of PPAR-gamma was higher in 8w- and 16w-old SHR than in age matched WKY. PPAR-gamma expression in 24w-old SHR did not increase and it was equal to 16w-old SHR and 24w-old WKY.
  
  
  CONCLUSIONS
  These datas on SHR suggest that the expression of PPAR-gamma changes with age and the development of high blood pressure. PPAR-gamma expression may play a compensatory role in hypertension, but this compensatory action is limited.
authors.affiliation: Main Laboratory of Environment and Genes Related to Disease, Ministry of Education, Department of Cardiology, Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Zheng-Li
authors.initials: ZL
authors.lastname: Di
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Niu
authors.forename: Jin
authors.initials: J
authors.lastname: Wei
authors.forename: Yong-Qin
authors.initials: YQ
authors.lastname: Li
authors.forename: Gai-Ling
authors.initials: GL
authors.lastname: Chen
authors.forename: Nan-Ping
authors.initials: NP
authors.lastname: Wang
mesh_terms: D000367:Age Factors; D000818:Animals; D001011:Aorta / Q000166:cytology; D049109:Cell Proliferation; D002478:Cells, Cultured; D006973:Hypertension / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D047492:Peroxisome Proliferator-Activated Receptors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
chemical_list: D047492:Peroxisome Proliferator-Activated Receptors; D012333:RNA, Messenger