title: Octacalcium phosphate combined with collagen orthotopically enhances bone regeneration.
abstract: Octacalcium phosphate (OCP) is resorbable bone regenerative material, but its brittleness makes it difficult to maintain its shape without restraint. We have engineered a scaffold constructed of synthetic OCP and porcine collagen sponge (OCP/Collagen) and investigated whether OCP/Collagen composite could improve bone regeneration. To examine this hypothesis, bone regeneration by the implantation of OCP/Collagen was compared with those by OCP and collagen. Radiographic and histological examination was performed and the percentage of newly formed bone (n-Bone%) in the defect was determined by a histomorphometrical analysis. OCP/Collagen, OCP, or collagen was implanted into the critical-sized defects in rat crania and fixed at 2, 4, or 8 weeks after implantation. OCP/Collagen improved the handling performance than the granules of OCP, and synergistically enhanced the bone regeneration beyond expectation, which were composed of bone nucleation by OCP and cell infiltration by collagen. Histomorphometrical analysis showed that n-Bone% +/- standard error treated with OCP/Collagen (48.4 +/- 5.14) was significantly higher than those with OCP (27.6 +/- 4.04) or collagen (27.4 +/- 5.69) in week 8. The present study suggests that the combination OCP with collagen elicited the synergistic effect for bone regeneration.
authors.affiliation: Division of Clinical Cell Therapy, Department of Translational Research, Center for Translational and Advanced Animal Research, Tohoku University School of Medicine, 2-1 Seiryo-Machi, Aoba-Ku, Sendai 980-8574, Japan. kamakura@mail.tains.tohoku.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Kamakura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sasaki
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Honda
authors.forename: Takahisa
authors.initials: T
authors.lastname: Anada
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D003094:Collagen / Q000737:chemistry; D008297:Male; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C022045:octacalcium phosphate; D003094:Collagen