[Study on the purification and scavenging free radical activity of water soluble polysaccharide of leave in Hippohae rhamnoides L].
The crude polysaccharide was extracted from Leave in Hippohae rhamnoides L. with hot water, and precipitated by ethanol. The crude polysaccharide has been fractionated by acidic ethanol. Three fractions (SJ1, SJ2, SJ3) were got respectively. SJ2 deproteinizationed by the combined methods of enzyme and Seveage, purified by DEAE-Sephadex A-25 gel filtration. PC analysis indicated that SJ22 is composed of Xyl,Ara,Glc,Gal,GaL A. The identification of purify by Sepharose CL-4B, paper chromatography and cellulous acetate electrophoresis showed it was homogeneous. Typical absorption of polysaccharides was shown in its IR spectrum. It contained a-glucosidic bonds by IR analysis. It had typical absorption of protein by UV scaning. SJ22 is first isolated from Leave in Hippohae rhamnoides L Scavenging free radical experiment showed that SJ22 was effective in scavenging superoxideradical and hydroxylradical, but only a little effective in scavenging lipid radical.
Liu
Chun-lan
CL
College of Life and Environmental Science, Central University for Nationalitites, Beijing 100081, China. Liuchunlan@cun.edu.cn
Liu
Hai-qing
HQ
Deng
Yi-hong
YH
Liu
Ming-liang
ML
D002854:Chromatography, Paper; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D031281:Hippophae / Q000737:chemistry*; D006358:Hot Temperature; D008970:Molecular Weight; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D012995:Solubility; D014867:Water / Q000737:chemistry
D016166:Free Radical Scavengers; D005609:Free Radicals; D011134:Polysaccharides; D014867:Water