CTCF mediates interchromosomal colocalization between Igf2/H19 and Wsb1/Nf1.
Gene transcription may be regulated by remote enhancer or insulator regions through chromosome looping. Using a modification of chromosome conformation capture (3C) and fluorescence in situ hybridization, we found that one allele of the insulin-like growth factor 2 (Igf2)/H19 imprinting control region (ICR) on chromosome 7 colocalized with one allele of Wsb1/Nf1 on chromosome 11. Omission of CCCTC-binding factor (CTCF) or deletion of the maternal ICR abrogated this association and altered Wsb1/Nf1 gene expression. These findings demonstrate that CTCF mediates an interchromosomal association, perhaps by directing distant DNA segments to a common transcription factory, and the data provide a model for long-range allele-specific associations between gene regions on different chromosomes that suggest a framework for DNA recombination and RNA trans-splicing.
Ling
Jian Qun
JQ
Medical Service, Department of Veterans Affairs, Palo Alto Health Care System, and Department of Medicine, Stanford University, Palo Alto, CA 94304, USA.
Li
Tao
T
Hu
Ji Fan
JF
Vu
Thanh H
TH
Chen
Hui Ling
HL
Qiu
Xin Wen
XW
Cherry
Athena M
AM
Hoffman
Andrew R
AR
D000483:Alleles; D000818:Animals; D001665:Binding Sites; D000076246:CCCTC-Binding Factor; D047369:Chromatin Immunoprecipitation; D033481:Chromosomes, Mammalian / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004843:Epistasis, Genetic; D005260:Female; D005786:Gene Expression Regulation; D018392:Genomic Imprinting; D017404:In Situ Hybridization, Fluorescence; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D025542:Neurofibromin 1 / Q000235:genetics; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics; D050436:Regulatory Elements, Transcriptional; D012097:Repressor Proteins / Q000378:metabolism*; D014158:Transcription, Genetic; D044767:Ubiquitin-Protein Ligases / Q000235:genetics
D000076246:CCCTC-Binding Factor; C000619698:Ctcf protein, mouse; D004268:DNA-Binding Proteins; C094335:H19 long non-coding RNA; D047908:Intracellular Signaling Peptides and Proteins; D025542:Neurofibromin 1; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D012097:Repressor Proteins; C503398:Wsb1 protein, mouse; D007335:Insulin-Like Growth Factor II; D044767:Ubiquitin-Protein Ligases