title: A polysaccharopeptide complex and a condensed tannin with antioxidant activity from dried rose (Rosa rugosa) flowers.
abstract: In this study, the fraction (P) from an aqueous extract of dried rose (Rosa rugosa) flowers was obtained by ethanol precipitation. P was chromatographed on DEAE-cellulose. The components retained on DEAE-cellulose were eluted with a linear gradient of 0-2 M NaCl solution. Two fractions, eluted at concentrations of 0.5 M NaCl and 1 M NaCl, respectively, were obtained. These two components were designated as P1 and P2, respectively. P1 was further purified using gel filtration on Sephadex G-200. P(1) yielded two peaks, and the two components were designated as P(1-a) and P(1-b), respectively. P(1-a) was a polysaccharide-peptide complex, and P(1-b) exhibited chemical properties of a condensed tannin as revealed by FTIR and NMR assay of carbohydrate and protein contents and HPLC-ESI-MS. The molecular masses of P(1-a) and P(1-b) were 150 kDa and 8 kDa, respectively. Both P(1-a) and P(1-b) possessed antioxidant activity, with the activity of P(1-b) higher than that of P(1-a). This study demonstrated that different components from rose flowers exhibited antioxidant activity.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
authors.forename: Z F
authors.initials: ZF
authors.lastname: Pi
authors.forename: H
authors.initials: H
authors.lastname: Yue
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.forename: M
authors.initials: M
authors.lastname: Fu
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Hou
authors.forename: L S
authors.initials: LS
authors.lastname: Shi
authors.forename: R R
authors.initials: RR
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: F
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D035264:Flowers; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011509:Proteoglycans / Q000302:isolation & purification* / Q000494:pharmacology; D027826:Rosa; D013634:Tannins / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011509:Proteoglycans; D013634:Tannins; C093216:polysaccharide peptide