Protection of peroxynitrite-induced DNA damage by dietary antioxidants.
The present study was undertaken to test the hypothesis that dietary antioxidants protect DNA damage induced by peroxynitrite, a potent physiological inorganic toxin. The present study showed that dietary antioxidants such as (-)-epigallocatechin gallate, quercerin, rutin, resveratrol, and ursolic acid inhibit single strand breaks in supercoiled plasmid DNA induced by 3-morpholinosydnomine N-ethylcarbamide (SIN-1), a generator of peroxynitrite through the reaction between nitric oxide and superoxide anion. The formation of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in calf thymus DNA by SIN-1 was also inhibited by dietary antioxidants. When U937 cells were incubated with 1 mM SIN-1 bolus, a significant increase of 8-OH-dG level was observed. However, oxidative DNA damage was significantly lower in the cells pre-treated with dietary antioxidants when cells were exposed to SIN-1.
Moon
Hye Kyung
HK
School of Life Sciences and Biotechnology and Agro-Biotechnology Education Center, Kyungpook National University, Taegu, Korea.
Yang
Eun Sun
ES
Park
Jeen Woo
JW
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D004032:Diet; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D008981:Molsidomine / Q000031:analogs & derivatives; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology; D014315:Triterpenes / Q000494:pharmacology; D020298:U937 Cells; D000097245:Ursolic Acid
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002392:Catechin; D004247:DNA; D003849:Deoxyguanosine; D016166:Free Radical Scavengers; D008981:Molsidomine; D030421:Peroxynitrous Acid; D000077185:Resveratrol; D013267:Stilbenes; D014315:Triterpenes; D000097245:Ursolic Acid; C002385:linsidomine; C427203:calf thymus DNA; C045651:epigallocatechin gallate