title: High-resolution radiation hybrid map of wheat chromosome 1D.
abstract: Physical mapping methods that do not rely on meiotic recombination are necessary for complex polyploid genomes such as wheat (Triticum aestivum L.). This need is due to the uneven distribution of recombination and significant variation in genetic to physical distance ratios. One method that has proven valuable in a number of nonplant and plant systems is radiation hybrid (RH) mapping. This work presents, for the first time, a high-resolution radiation hybrid map of wheat chromosome 1D (D genome) in a tetraploid durum wheat (T. turgidum L., AB genomes) background. An RH panel of 87 lines was used to map 378 molecular markers, which detected 2312 chromosome breaks. The total map distance ranged from approximately 3,341 cR(35,000) for five major linkage groups to 11,773 cR(35,000) for a comprehensive map. The mapping resolution was estimated to be approximately 199 kb/break and provided the starting point for BAC contig alignment. To date, this is the highest resolution that has been obtained by plant RH mapping and serves as a first step for the development of RH resources in wheat.
authors.affiliation: Department of Bioscience & Biotechnology, Drexel University, Philadelphia, Pennsylvania 19141, USA.
authors.forename: Venu
authors.initials: V
authors.lastname: Kalavacharla
authors.forename: Khwaja
authors.initials: K
authors.lastname: Hossain
authors.forename: Yong
authors.initials: Y
authors.lastname: Gu
authors.forename: Oscar
authors.initials: O
authors.lastname: Riera-Lizarazu
authors.forename: M Isabel
authors.initials: MI
authors.lastname: Vales
authors.forename: Suresh
authors.initials: S
authors.lastname: Bhamidimarri
authors.forename: Jose L
authors.initials: JL
authors.lastname: Gonzalez-Hernandez
authors.forename: Shivcharan S
authors.initials: SS
authors.lastname: Maan
authors.forename: Shahryar F
authors.initials: SF
authors.lastname: Kianian
mesh_terms: D019457:Chromosome Breakage; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D020224:Expressed Sequence Tags; D011123:Polyploidy; D021101:Radiation Hybrid Mapping; D014908:Triticum / Q000235:genetics*