title: Mechanical properties of single living cells encapsulated in polyelectrolyte matrixes.
abstract: We have studied the mechanical properties of encapsulated Saccharomyces cerevisiae yeast cells by performing AFM force measurements. Single living cells have been coated through the alternate deposition of oppositely charged polyelectrolyte layers and mechanically trapped into a porous membrane. Coated and uncoated cells in presence/absence of bud scars, i.e. scars resulting from previous budding events, have been investigated. No significant differences between encapsulated and bare cells could be inferred from AFM topographs. On the other hand, investigation on the system elasticity through the acquisition and analysis of force curves allowed us to put in evidence the differences in the mechanical properties between the hybrid cell/polyelectrolyte system and the uncoated cells. Analysis of the curves contact region indicates that the polyelectrolyte coating increases the system rigidity. Quantitative evaluation of the cell rigidity through the Hertz-Sneddon model showed that coated cells are characterized by a Young's modulus higher than the value obtained for uncoated cells and similar to the value observed on the bud scar region of uncoated cells.
authors.affiliation: Physics Department, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Svaldo Lanero
authors.forename: Ornella
authors.initials: O
authors.lastname: Cavalleri
authors.forename: Silke
authors.initials: S
authors.lastname: Krol
authors.forename: Ranieri
authors.initials: R
authors.lastname: Rolandi
authors.forename: Alessandra
authors.initials: A
authors.lastname: Gliozzi
mesh_terms: D001696:Biomechanical Phenomena / Q000295:instrumentation; D018914:Cells, Immobilized / Q000502:physiology; D018625:Microscopy, Atomic Force / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology*; D013314:Stress, Mechanical