title: Permeability of ovine primordial follicles to different cryoprotectants.
abstract: OBJECTIVE
  To determine the behavior of isolated primordial follicles that were exposed to different concentrations of dimethyl sulfoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH), and glycerol (GLY).
  
  
  METHODS
  Isolated primordial follicles were exposed to the cryoprotectant (CPA) solution and photographed to calculate their volume at different periods of exposure.
  
  
  METHODS
  Laboratorio Renzo Giuliani, University of Florence, Italy.
  
  
  METHODS
  Lambs, 30-40 days old.
  
  
  METHODS
  Isolation of primordial follicles and subsequent exposure to CPA.
  
  
  METHODS
  Follicular volume.
  
  
  RESULTS
  At 2 minutes of CPA exposure, all follicles appeared to be shrunken. At approximately 5 minutes, shrinkage ceased, and follicles started to swell, absorbing the CPA and water to maintain osmotic equilibrium. When DMSO was tested, follicular dehydration in all concentrations did not exceed 17%; with PROH and EG, it reached 33% and 27%, respectively. The highest degree of dehydration (48%) was seen with GLY. In almost all tested concentrations, follicular shrinkage occurred up to 5 minutes.
  
  
  CONCLUSIONS
  Volume changes in isolated primordial follicles can fluctuate according to the CPA used and its concentration.
authors.affiliation: Hospital Veterinário Prédio 97, University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil. amorimchris@yahoo.com
authors.forename: Christiani Andrade
authors.initials: CA
authors.lastname: Amorim
authors.forename: Davide
authors.initials: D
authors.lastname: Rondina
authors.forename: Carolina Madeira
authors.initials: CM
authors.lastname: Lucci
authors.forename: Paulo Bayard Dias
authors.initials: PB
authors.lastname: Gonçalves
authors.forename: José Ricardo de
authors.initials: JR
authors.lastname: Figueiredo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Giorgetti
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D003451:Cryoprotective Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D019610:Cytoprotection / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D019188:Organ Preservation Solutions / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006080:Ovarian Follicle / Q000502:physiology*; D010539:Permeability / Q000187:drug effects; D012756:Sheep
chemical_list: D003451:Cryoprotective Agents; D019188:Organ Preservation Solutions