Coenzyme Q10 modulates cognitive impairment against intracerebroventricular injection of streptozotocin in rats.
Coenzyme Q10 (CoQ10), a peculiar lipophilic antioxidant, is an essential component of the mitochondrial electron-transport chain. It is involved in the manufacturing of adenosine triphosphate (ATP) and has been linked with improving cognitive functions. The present study shows the neuroprotective effect of CoQ10 on cognitive impairments and oxidative damage in hippocampus and cerebral cortex of intracerebroventricular-streptozotocin (ICV-STZ) infused rats. Male Wistar rats (1-year old) were infused bilaterally with an ICV injection of STZ (1.5 mg/kg b.wt., in normal saline), while sham group received vehicle only. After 24 h, the rats were supplemented with CoQ10 (10 mg/kg b.wt. i.p.) for 3 weeks. The learning and memory tests were monitored 2 weeks after the lesioning. STZ-infused rats showed the loss of cognitive performance in Morris water maze and passive avoidance tests. Three weeks after the lesioning, the rats were sacrificed for estimating the contents of thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), protein carbonyl (PC), ATP and the activities of glutathione peroxidase (GPx), glutathione reductase (GR), cholineacetyltransferase (ChAT) and acetylcholinesterase (AChE). Significant alteration in the markers of oxidative damage (TBARS, GSH, PC, GPx and GR) and a decline in the level of ATP were observed in the hippocampus and cerebral cortex of ICV-STZ rat. A significant decrease in ChAT activity and a concomitant increase in AChE activity were observed in the hippocampus. However, supplementation with CoQ10 in STZ-infused rats reversed all the parameters significantly. Thus, the study demonstrates that CoQ10 may have a therapeutic importance in the treatment of Alzheimer's type dementia.
Ishrat
Tauheed
T
Neurotoxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi, India.
Khan
M Badruzzaman
MB
Hoda
Md Nasrul
MN
Yousuf
Seema
S
Ahmad
Muzamil
M
Ansari
Mubeen A
MA
Ahmad
Abdullah S
AS
Islam
Fakhrul
F
D000110:Acetylcholinesterase / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology; D002540:Cerebral Cortex / Q000201:enzymology* / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003067:Coenzymes; D003072:Cognition Disorders / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D005978:Glutathione / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology* / Q000378:metabolism; D007276:Injections, Intraventricular; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000378:metabolism*; D010088:Oxidoreductases / Q000187:drug effects / Q000378:metabolism; D050050:Protein Carbonylation / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D013311:Streptozocin; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
D000975:Antioxidants; D003067:Coenzymes; D018696:Neuroprotective Agents; D017392:Thiobarbituric Acid Reactive Substances; D014451:Ubiquinone; D013311:Streptozocin; D000255:Adenosine Triphosphate; D010088:Oxidoreductases; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; C024989:coenzyme Q10; D005978:Glutathione