Effects of mycelial culture of Phellinus linteus on ethanol-induced gastric ulcer in rats.
The gastroprotective effects of a mycelial culture of Phellinus linteus (MCPL) were evaluated by determining the ulcer index, gastric mucus content, histopathological observation and histochemical properties of mucin in an ethanol-induced ulcer model of rats. Preadministration with MCPL at doses of 20 and 60 mg/kg, showed a significant decrease of bleeding and ulcer index and alleviated the histopathological changes induced by ethanol such as hemorrhage and necrosis. Ethanol treatment decreased the gastric adhesion mucus content, but a higher level of gastric mucus persisted after preadministration of MCPL. As for the histochemical properties of mucins, marked changes were observed in both the surface and gland mucous cells in ethanol-treated rats, but these changes were detected only in the surface mucous cells in rat preadministered with MCPL. Using conventional methods for mucins, ethanol-treated rats revealed a decrease of neutral and acid mucin in the surface epithelium and mucous neck cells compared with normal rats. A marked decrease of BSL-1 by lectin histochemistry was also revealed in the ethanol-treated rats. But the MCPL preadministered rats showed similar stainabilities and lectin affinity patterns for mucins as the normal rats. These results indicate that pretreatment with MCPL provided protection of the gastric mucosa from ethanol-induced injury by maintaining the mucus barrier in rats.
Lee
Jun Hyuk
JH
Department of Anatomy, College of Oriental Medicine, Dong-Eui University, Busan, Republic of Korea.
Lee
Soo Jin
SJ
Choi
Yung Hyun
YH
Chung
Kyung Tae
KT
Jeong
Young Kee
YK
Choi
Byung Tae
BT
D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002478:Cells, Cultured; D000431:Ethanol; D048690:Fruiting Bodies, Fungal; D005752:Gastric Mucins / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D008297:Male; D003904:Mitosporic Fungi; D025282:Mycelium; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*
D000897:Anti-Ulcer Agents; D005752:Gastric Mucins; D010936:Plant Extracts; D000431:Ethanol