Regulation of TCRbeta gene assembly by a promoter/enhancer holocomplex.
Antigen receptor gene assembly is governed by transcriptional promoters and enhancers that communicate over large distances and modulate chromatin accessibility to V(D)J recombinase. The precise role of these cis-acting elements in opening chromatin at recombinase targets and the mechanisms underlying their crosstalk remain unclear. We show that the TCRbeta enhancer (Ebeta) directs long-range chromatin opening over both DbetaJbeta clusters. Strikingly, chromatin associated with the Dbeta1 gene segment is refractory to Ebeta-mediated opening. Accessibility at Dbeta1 is accompanied by the formation of a stable holocomplex between a Dbeta-proximal promoter and Ebeta. These findings indicate a stepwise process for Dbeta --> Jbeta recombination that relies on distinct aspects of Ebeta activity: an intrinsic function that directs general chromatin opening and a cooperative function that facilitates the assembly of a promoter/enhancer holocomplex, unmasks the Dbeta1 gene segment, and triggers TCRbeta gene assembly.
Oestreich
Kenneth J
KJ
Department of Microbiology and Immunology, Vanderbilt University, Nashville, Tennessee 37232, USA.
Cobb
Robin Milley
RM
Pierce
Steven
S
Chen
Jianzhu
J
Ferrier
Pierre
P
Oltz
Eugene M
EM
D000818:Animals; D002460:Cell Line; D004742:Enhancer Elements, Genetic; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics*; D019674:Genes, T-Cell Receptor beta; D051379:Mice; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D013601:T-Lymphocytes / Q000276:immunology*; D014158:Transcription, Genetic