[Production purification and characterization of polyantibodies against microcystin-LR].
OBJECTIVE
Production and characterization the polyantibodies against MCYST-LR.


METHODS
microcystin-LR (MC-LR) was conjugated to KLH, then immune the rabbit by the routine method. Purify the antiserum by centrifugal and saturated sulfuric ammonium precipitated methods. Count the affinity constant and measure the titers, IC50 Value and the sensitivity.


RESULTS
The titers of the antiserum is over 25600. IC50 value is about from 0.1 ng/ml to 1 ng/ml. The across-reactivity ratio to MC-LW and MC-LR is about from 0.1% to 20%.
Zhao
Xiao-Lian
XL
School of Food Science of Southern Yangtze University, Wuxi 214036, China.
Sun
Wei-Rong
WR
Liu
Yuan
Y
Liu
Xian-Jin
XJ
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification*; D008297:Male; D008387:Marine Toxins; D052998:Microcystins / Q000276:immunology*; D011817:Rabbits
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR