title: Src homology 2 domain of overexpressed Lyn kinase is responsible for the acceleration of granulocyte colony-stimulating factor-induced neutrophilic nuclear lobulation.
abstract: We have previously shown that the overexpression of a Src family kinase, Lyn, and its kinase-negative form, LynKN, in a granulocyte progenitor cell line, GM-I62M, accelerates neutrophilic nuclear lobulation when the cells are cultured in the presence of granulocyte colony-stimulating factor. In this study, we investigated the role of the Src homology 2 (SH2) and SH3 domains of Lyn in the accelerated induction of nuclear lobulation. In contrast to wild-type Lyn, the overexpression of its SH2 domain mutant did not induce the accelerated nuclear morphological changes, but the overexpressed SH3 domain mutant had the same effects as wild-type Lyn. Therefore, the SH2 domain of Lyn is responsible for the accelerated induction of neutrophilic nuclear lobulation upon G-CSF stimulation.
authors.affiliation: Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, Okayama 700-8530, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Oka
authors.forename: Akiko
authors.initials: A
authors.lastname: Suzuki
authors.forename: Tomomi
authors.initials: T
authors.lastname: Omura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D042381:Granulocyte Precursor Cells / Q000201:enzymology* / Q000648:ultrastructure*; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism; D014162:Transfection; D018909:src Homology Domains; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D010750:Phosphoproteins; D016179:Granulocyte Colony-Stimulating Factor; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases