Grafting of poly(ethylene glycol) onto poly(acrylic acid)-coated glass for a protein-resistant surface.
The surface of solid glass supports for samples in optical microscopy and for biosensors needs to be protein-resistant. A coating of a poly(ethylene glycol) monomethyl ether (mPEG) on the surface of the glass is one promising method for preventing the nonspecific adsorption of proteins. In this study, we have developed a novel technique for achieving an optimal coverage of a glass surface with mPEG to prevent protein adhesion. A clean glass substrate previously treated with (3-aminopropyl)dimethylethoxysilane (APDMES) was treated sequentially with poly(acrylic acid) and subsequently a primary amine derivative of mPEG in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. The resultant glass surface was demonstrated to be highly protein-resistant, and the adsorption of bovine serum albumin decreased to only a few percentage points of that on a glass surface treated with APDMES alone. Furthermore, to extend the present method, we also prepared a glass substrate on which biotinylated poly(ethylene glycol) was cografted with mPEG, and biotinylated myosin subfragment-1 (biotin-S1) was subsequently immobilized on this substrate by biotin/avidin chemistry. Actin filaments were observed to glide on the biotin-S1-coated glass surface in the presence of ATP, and thus, the method is capable of immobilizing the protein specifically without any loss in its biological function.
Wazawa
Tetsuichi
T
OMRON-Endowed Chair in Nano Optical Devices, and Soft Biosystem Laboratory, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871 Japan. wazawa@fbs.osaka-u.ac.jp
Ishizuka-Katsura
Yoshiko
Y
Nishikawa
So
S
Iwane
Atsuko Hikikoshi
AH
Aoyama
Shigeru
S
D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D002234:Carbodiimides / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D005898:Glass; D018625:Microscopy, Atomic Force; D015879:Myosin Subfragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry*; D020005:Propanols / Q000737:chemistry; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties
D000180:Acrylic Resins; D001672:Biocompatible Materials; D002234:Carbodiimides; D004800:Enzymes, Immobilized; D015879:Myosin Subfragments; D020005:Propanols; D012821:Silanes; C114992:dimethylaminopropanol; D011092:Polyethylene Glycols; C006903:carbopol 940; D001710:Biotin; C028210:monomethoxypolyethylene glycol