Aminoacyl RNA domain of turnip yellow mosaic virus Val-RNA interacting with elongation factor Tu.
Turnip yellow mosaic virus (TYMV) Val-RNA forms a complex with the peptide elongation factor Tu (EF-Tu) in the presence of GTP: the Val-RNA is protected by EF-Tu.GTP from non-enzymatic deacylation and nuclease digestion. The determination of the length of the shortest TYMV Val-RNA fragment that binds EF-Tu.GTP leads us to conclude that the valylated aminoacyl RNA domain equivalent in tRNAs to the continuous helix formed by the acceptor stem and the T arm is sufficient for complex formation. Since the aminoacyl RNA domain is also sufficient for adenylation by the ATP(CTP):tRNA nucleotidyltransferase, an analogy can be drawn between these two tRNA-specific proteins.
Joshi
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Institut Jacques Monod, CNRS and Université Paris VII, 2 Place Jussieu, 75251 Paris Cedex 05, France.
Faulhammer
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Chapeville
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Sprinzl
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Haenni
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