title: A genetic interaction between the vesicular acetylcholine transporter VAChT/UNC-17 and synaptobrevin/SNB-1 in C. elegans.
abstract: Acetylcholine, a major excitatory neurotransmitter in Caenorhabditis elegans, is transported into synaptic vesicles by the vesicular acetylcholine transporter encoded by unc-17. The abnormal behavior of unc-17(e245) mutants, which have a glycine-to-arginine substitution in a transmembrane domain, is markedly improved by a mutant synaptobrevin with an isoleucine-to-aspartate substitution in its transmembrane domain. These results suggest an association of vesicular soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) components with vesicular neurotransmitter transporters.
authors.affiliation: Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Gisela M
authors.initials: GM
authors.lastname: Sandoval
authors.forename: Janet S
authors.initials: JS
authors.lastname: Duerr
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hodgkin
authors.forename: James B
authors.initials: JB
authors.lastname: Rand
authors.forename: Gary
authors.initials: G
authors.lastname: Ruvkun
mesh_terms: D000818:Animals; D001120:Arginine / Q000235:genetics; D001522:Behavior, Animal; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D005455:Fluorescent Antibody Technique / Q000379:methods; D007532:Isoleucine / Q000235:genetics; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology; D009154:Mutation / Q000502:physiology; D050683:R-SNARE Proteins / Q000235:genetics*; D050494:Vesicular Acetylcholine Transport Proteins / Q000235:genetics*
chemical_list: D029742:Caenorhabditis elegans Proteins; D050683:R-SNARE Proteins; C082365:Unc-17 protein, C elegans; D050494:Vesicular Acetylcholine Transport Proteins; D007532:Isoleucine; D001120:Arginine