Glycolytic sequence and respiration of Debaryomyces hansenii as compared to Saccharomyces cerevisiae.
The fermentation and respiration activities of Debaryomyces hansenii were compared with those of Saccharomyces cerevisiae grown to stationary phase with high respiratory activity. It was found that: (a) glucose consumption, fermentation and respiration were lower than for S. cerevisiae; (b) fasting produced a much smaller decrease of respiration; (c) glucose consumed and not transformed to ethanol was higher; (d) in S. cerevisiae, full oxygenation prevented ethanol production but this effect was reversed by CCCP, whereas D. hansenii still showed some ethanol production under aerobiosis, which was moderately increased by CCCP. ATP levels were similar in the two yeasts. Levels of glycolytic intermediaries after glucose addition, and enzyme activities, indicated that the main difference and limiting step to explain the lower fermentation of D. hansenii is phosphofructokinase activity. Respiration and fermentation, which are lower in D. hansenii, compete for the re-oxidation of reduced nicotinamide adenine nucleotides; this competition, in turn, seems to play a role in defining the fermentation rates of the two yeasts. The effect of CCCP on glucose consumption and ethanol production also indicates a role of ADP in both the Pasteur and Crabtree effects in S. cerevisiae but not in D. hansenii. D. hansenii shows an alternative oxidase, which in our experiments did not appear to be coupled to the production of ATP.
Sánchez
N S
NS
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, D. F. 04510, México.
Calahorra
M
M
González-Hernández
J C
JC
Peña
A
A
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006019:Glycolysis / Q000502:physiology; D006593:Hexokinase / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology*; D025221:Phosphofructokinases / Q000378:metabolism; D011767:Pyruvate Decarboxylase / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D004718:Saccharomycetales / Q000201:enzymology / Q000378:metabolism*; D014475:Uncoupling Agents / Q000494:pharmacology
D014475:Uncoupling Agents; D009243:NAD; D000431:Ethanol; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000426:Alcohol Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D025221:Phosphofructokinases; D006593:Hexokinase; D011767:Pyruvate Decarboxylase; D005634:Fructose-Bisphosphate Aldolase; D005947:Glucose