title: A new cell line from the wax moth Galleria mellonella Linne (Lepidoptera: Pyralididae).
abstract: A cell line derived from the larval-fat body tissues of the wax moth, Galleria mellonella Linne, was established in MGM-450 medium. The cells grew in suspension and were mainly spherical in shape. Population doubling time was between 1.4 and 1.7 d over a range of 15 to 35 degrees C, and the maximum growth rate was at 25 degrees C. The chromosome number ranged from 70-239, with a mode of 170. The cells were sensitive to 20-hydroxyecdysone, which stimulated their growth and induced morphological changes. The cell line was designated GaMe-LF1.
authors.affiliation: Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509 Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Eguchi
authors.forename: Kikuo
authors.initials: K
authors.lastname: Iwabuchi
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D003470:Culture Media; D004441:Ecdysterone / Q000378:metabolism; D005216:Fat Body / Q000166:cytology*; D009036:Moths
chemical_list: D003470:Culture Media; D004441:Ecdysterone