title: Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section.
abstract: Received October 14, 2005; revised January 7, 2006; accepted January 9, 2006; posted January 12, 2006 (Doc. ID 65391) The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wavelength in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.
authors.affiliation: Graduate Institute of Electro-Optical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Tsung-Han
authors.initials: TH
authors.lastname: Tsai
authors.forename: Cheng-Yung
authors.initials: CY
authors.lastname: Lin
authors.forename: Huai-Jen
authors.initials: HJ
authors.lastname: Tsai
authors.forename: Szu-Yu
authors.initials: SY
authors.lastname: Chen
authors.forename: Shih-Peng
authors.initials: SP
authors.lastname: Tai
authors.forename: Kung-Hsuan
authors.initials: KH
authors.lastname: Lin
authors.forename: Chi-Kuang
authors.initials: CK
authors.lastname: Sun
mesh_terms: D000818:Animals; D001704:Biopolymers / Q000378:metabolism; D006321:Heart / Q000196:embryology*; D007089:Image Enhancement / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D014018:Tissue Distribution; D015027:Zebrafish; D000097573:Red Fluorescent Protein
chemical_list: D001704:Biopolymers; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein