Quantitative measurement of molecular diffusion coefficients by NMR spectroscopy.
An offset-independent adiabatic inversion pulse is used in the diffusion experiment to uniformly excite a sample region that is sufficiently long to ignore the ending effects, yet is short enough to have a homogeneous RF field and to represent the pulsed field gradient with a linear approximation. Under these conditions, the diffusion decay of the peak intensity appears to be Gaussian as a function of the effective gradient field ge as if all the molecules inside the selected region experienced the same ge. Quantitative measurement of molecular diffusion coefficients is therefore made possible.
Zhang
Shanmin
S
Sealy Center for Structural Biology, Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-1157, USA. shanminz@hotmail.com
D004058:Diffusion; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D014867:Water / Q000737:chemistry
D014867:Water