title: Synthesis of a library of polycationic lipid core dendrimers and their evaluation in the delivery of an oligonucleotide with hVEGF inhibition.
abstract: This article follows on from our previous work in the area of non-viral gene delivery using polycationic dendrimers (PCDs). Herein we report on the synthesis and efficacy of a new library of lipid core PCDs in the delivery of the anti-angiogenic oligonucleotide (ODN-1) to retinal pigment epithelial cells. ELISA was used to monitor hVEGF levels in cells transfected with dendriplexes, Cytofectin GSV and control (non-transfected). At 48 h, hVEGF titres had returned to that of the untransfected control for Cytofectin GSV however, a number of dendriplexes continued to exhibit a marked reduction in hVEGF titres.
authors.affiliation: School of Pharmacy, The University of Queensland, St Lucia, Qld 4072, Australia. hparekh@pharmacy.uq.edu.au
authors.forename: Harendra S
authors.initials: HS
authors.lastname: Parekh
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Marano
authors.forename: Elizabeth P
authors.initials: EP
authors.lastname: Rakoczy
authors.forename: Joanne
authors.initials: J
authors.lastname: Blanchfield
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry; D016503:Drug Delivery Systems; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage* / Q000235:genetics; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D050091:Dendrimers; D008055:Lipids; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A