title: Determinants of vector specificity of o'nyong nyong and chikungunya viruses in Anopheles and Aedes mosquitoes.
abstract: The alphaviruses o'nyong nyong virus (ONNV) and chikungunya virus (CHIKV) provide a unique system to study the viral genes involved in vector specificity. ONNV infects both anopheline and culicine mosquitoes, whereas CHIKV infects only culicine mosquitoes. In this study, chimeric viruses were constructed that contained genes from both ONNV and CHIKV. These chimeras and previously described full-length infectious clones of ONNV and CHIKV were evaluated in Anopheles gambiae and Aedes aegypti mosquitoes. Virus derived from the infectious clones of ONNV and CHIKV retained the vector specificity of the parental viruses. All six of the chimeras were found to infect Ae. aegypti mosquitoes at high rates but only the chimera containing viral genes encoding all of the structural proteins of ONNV was able to infect An. gambiae mosquitoes. These data indicate that all of the viral structural proteins are necessary for ONNV to infect An. gambiae mosquitoes.
authors.affiliation: Department of Pathology, University of Texas Medical Branch, Galveston 77555-0609, USA. dlvanlan@utmb.edu
authors.forename: Dana L
authors.initials: DL
authors.lastname: Vanlandingham
authors.forename: Konstantin
authors.initials: K
authors.lastname: Tsetsarkin
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Klingler
authors.forename: Chao
authors.initials: C
authors.lastname: Hong
authors.forename: Kate L
authors.initials: KL
authors.lastname: McElroy
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Lehane
authors.forename: Stephen
authors.initials: S
authors.lastname: Higgs
mesh_terms: D000330:Aedes / Q000821:virology*; D000524:Alphavirus / Q000502:physiology*; D000818:Animals; D000852:Anopheles / Q000821:virology*; D002646:Chikungunya virus / Q000502:physiology*; D002678:Chimera; D005260:Female; D007303:Insect Vectors / Q000821:virology*; D008969:Molecular Sequence Data