title: A new tool for experimental tumour research.
abstract: Surgical resected tumours are often stored for hours in the clinic upon transfer to the bench leading to apoptosis of tumour cells making them no longer suitable for molecular analysis and diagnostic procedures. The way out of this problem may be a new oxygen-enriched solution (OES). We tested this agent using surgical resections of carcinomas of lung, rectum and pancreas. Immediately after resection, one part of each individual tumour was stored in PBS and the other part in OES, and the content of viable or dead cells was determined by trypan blue exclusion and MTT-assay. We found that OES keeps tumour cells up to 3 days and longer more viable than PBS and reduces the percentage of dead cells without inducing therapy resistance and affecting the outcome of experimental procedures. Thus, storing freshly resected tumours in OES may save time for tumour transfer and initiation of experiments.
authors.affiliation: Molecular Urooncology, German Cancer Research Center, Heidelberg, Germany.
authors.forename: Chengwen
authors.initials: C
authors.lastname: Zhang
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Gassler
authors.forename: Philipp A
authors.initials: PA
authors.lastname: Schnabel
authors.forename: Hendrik
authors.initials: H
authors.lastname: Dienemann
authors.forename: Armin
authors.initials: A
authors.lastname: Kolb
authors.forename: Peter
authors.initials: P
authors.lastname: Buchler
authors.forename: Markus
authors.initials: M
authors.lastname: Buchler
authors.forename: Helmut
authors.initials: H
authors.lastname: Friess
authors.forename: Ingrid
authors.initials: I
authors.lastname: Herr
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D035843:Biomedical Research; D015687:Cell Hypoxia; D002945:Cisplatin / Q000494:pharmacology; D015925:Cryopreservation; D006801:Humans; D009374:Neoplasms, Experimental / Q000628:therapy*; D019188:Organ Preservation Solutions; D010100:Oxygen / Q000378:metabolism*; D012107:Research Design; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D019188:Organ Preservation Solutions; D002945:Cisplatin; D010100:Oxygen