Cleavage of the N-linked oligosaccharide from the surfaces of Chlamydia species affects infectivity in the mouse model of lung infection.
Previous studies determined that the Chlamydia glycan contains a high-mannose oligosaccharide, which is involved in attachment and infectivity of the organism, and that removal of the glycan decreases infectivity in vitro. The present study demonstrates that treatment of the organism with N-glycanase decreases or ablates infectivity in vivo.
Campbell
Lee Ann
LA
Department of Pathobiology, University of Washington, Box 357238, Seattle, WA 98195, USA. lacamp@u.washington.edu
Lee
Amy
A
Kuo
Cho-chou
CC
D000818:Animals; D002690:Chlamydia Infections / Q000188:drug therapy / Q000209:etiology / Q000517:prevention & control*; D008171:Lung Diseases / Q000517:prevention & control*; D051379:Mice; D009844:Oligosaccharides / Q000378:metabolism*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000494:pharmacology; D011134:Polysaccharides / Q000502:physiology*
D009844:Oligosaccharides; D011134:Polysaccharides; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase