title: [Determination of cotinine in human urine with gas chromatograph-mass spectrometry].
abstract: OBJECTIVE
  To establish the method of gas chromatograph-mass spectrometry (GC-MS) for the determination of the cotinine (COT) in human urine.
  
  
  METHODS
  The conjugated trans-3'-hydroxycotinine (THOC) and COT were hydrolyzed in human urine with beta-glucuronidase. The composition of COT was extracted with the mixture of dichloromethane and n-butyl acetate (2:1) and was separated with HP-5MS fused-silica capillary column. The GC-MS was used for determining its content.
  
  
  RESULTS
  The monitoring limit of this method was 0.02 microg/L. Its recovery rate was higher than 90%, Its accuracy rate was 4.30%. It was used for the determination of the cotinine in human urine in Guangzhou Biological Bank the Elderly Cohort.
  
  
  CONCLUSIONS
  The GC-MS method is a good microanalysis for monitoring the cotinine in human urine rapidly and accurately with little background disturbance. It has been applied in our Guangzhou Cohort Study for determining cotinine in human urine.
authors.affiliation: Guangzhou Monitoring Centre of Occupational Health, Guangzhou 510620 China.
authors.forename: Min
authors.initials: M
authors.lastname: Cao
authors.forename: Yi-lun
authors.initials: YL
authors.lastname: Zhang
authors.forename: Chao-qiang
authors.initials: CQ
authors.lastname: Jiang
authors.forename: Tai-hing
authors.initials: TH
authors.lastname: Lam
authors.forename: Kar-keung
authors.initials: KK
authors.lastname: Cheng
authors.forename: Wei-sen
authors.initials: WS
authors.lastname: Zhang
mesh_terms: D003367:Cotinine / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D012680:Sensitivity and Specificity; D012907:Smoking / Q000652:urine
chemical_list: D003367:Cotinine