title: Characterization of RNASET2, the first human member of the Rh/T2/S family of glycoproteins.
abstract: Ribonucleases are ubiquitous enzymes involved in RNA metabolism and are classified in several families on the basis of their structural, catalytic, and biological properties. Here, we describe characterization of the only human member of the Rh/T2/S family of acid hydrolases so far described, named RNASET2. This protein was previously reported to have an interesting biological function in the control of tumourigenesis and metastatization. We show that RNASET2 is present in multiple forms in human cell lines and mouse tissues, one of which represents the full length, glycosylated and secreted form, while the others are proteolytic products. RNASET2 is endowed with catalytic activity as demonstrated with purified recombinant protein expressed in the Baculovirus Expression Vector System and in a human cell line ectopically expressing various types of constructs. Furthermore, we document for this protein a lysosomal localization as described for other members of the Rh/T2/S family of ribonucleases. The results presented herein represent a further advancement toward the molecular understanding of the tumour suppressive properties of the human RNASET2 protein.
authors.affiliation: Department of Biotechnology and Molecular Sciences, University of Insubria, Via JH Dunant, 3, 21100 Varese, Italy. paola.compomenosi@uninsubria.it
authors.forename: Paola
authors.initials: P
authors.lastname: Campomenosi
authors.forename: Silvia
authors.initials: S
authors.lastname: Salis
authors.forename: Christer
authors.initials: C
authors.lastname: Lindqvist
authors.forename: Davide
authors.initials: D
authors.lastname: Mariani
authors.forename: Tommy
authors.initials: T
authors.lastname: Nordström
authors.forename: Francesco
authors.initials: F
authors.lastname: Acquati
authors.forename: Roberto
authors.initials: R
authors.lastname: Taramelli
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004789:Enzyme Activation; D005260:Female; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D051379:Mice; D010051:Ovarian Neoplasms / Q000378:metabolism*; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry* / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins; D012260:Ribonucleases; C425232:RNASET2 protein, human