[Investigating the correlation between polymorphisms with couple sharing rate of TAP gene and hypertensive disorder complicating pregnancy].
OBJECTIVE
To investigate and clarify whether the genetic susceptibility to women with hypertensive disorder complicating pregnancy or pre-eclampsia is associated with polymorphisms and couple sharing rate of transporter associate with antigen processing genes(TAP).


METHODS
One hundred and two severe pre-eclampsia women and their spouses served as study group, and 200 normal pregnant women and their spouses were selected as control group. All pregnant women were primipara with single fetus. Genomic DNA was extracted from 2 mL cubital venous blood. We used the amplification refractory mutation system polymerase chain reaction(ARMS-PCR) to characterize TAP gene locus 333, 637, 379, 565, 665.


RESULTS
We observed eleven TAP haplotypes. There were four kinds of haplotypes(1A-1D) existing in TAP1, and seven kinds of haplotypes(2A-2G) existing in TAP2. The gene frequencies of TAP2B(Chi2=9.19, P<0.01, RR=4.18) and TAP2F(Chi2=5.34, P<0.05, RR=4.63) of patient group with pre-eclampsia were significantly higher as compared with control group. The analyses of some TAP haplotypes such as TAP1B(Chi2=4.87, P<0.05, RR=3.14), TAP1C(Chi2=5.42, P<0.05, RR=4.90), TAP2B(Chi2=9.65, P<0.01, RR=5.39) showed that the couple sharing rate of pre-eclampsia women and their spouses had statistically a highly significant increase in comparison with that of controls.


CONCLUSIONS
Our data suggest that the presence of TAP2B or TAP2F haplotypes should be considered as a risk increased to pregnant women being susceptible to hypertensive disorder complicating pregnancy; and also the elevated couple sharing rates of TAP1B, TAP1C and TAP2B genes will increase the opportunity or possibility of pregnant women suffering from pre-eclampsia disease.
Gao
Mei-yang
MY
Department of Obstestic, Second Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong, 510260 P.R.China. gaomy2000@21cn.com
Wu
Ai-hua
AH
Wen
Chun-xia
CX
She
Da-xian
DX
Chen
Sheng-qiang
SQ
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000328:Adult; D005191:Family Characteristics / Q000208:ethnology; D005260:Female; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000150:complications*; D008297:Male; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011248:Pregnancy Complications / Q000209:etiology* / Q000235:genetics*
D018528:ATP-Binding Cassette Transporters; C477327:transporter associated with antigen processing (TAP)