Determination of telmisartan in rat tissues by in-tube solid-phase microextraction coupled to high performance liquid chromatography.
A poly(methacrylic acid-ethylene glycol dimethacrylate, MAA-EGDMA) monolithic capillary was used for the direct and on-line extraction of telmisartan from Sprague-Dawley rat tissue (heart, kidney, and liver) homogenates. Under optimized conditions, the tissue homogenates were simply diluted with a mixture of phosphate buffer (pH 2)/ACN (90:8 v/v), and then injected for extraction only after centrifugation and filtration. Coupled to HPLC with fluorescence detection, the method was linear over the range of 1.25-1500 ng/g for telmisartan in heart and kidney, 12.5-15 000 ng/g in liver with correlation coefficients over 0.9992. The detection limits were found to be in the range from 0.24 to 1.8 ng/g. RSDs for intra- and inter-day ranged from 1.2 to 8.1%. The determination of telmisartan in treated rat tissues was achieved by using the proposed method.
Nie
Jing
J
Department of Chemistry, Wuhan University, Wuhan, P. R. China.
Zhao
Qun
Q
Huang
Jingfang
J
Xiang
Bingren
B
Feng
Yu-Qi
YQ
D047228:Angiotensin II Type 1 Receptor Blockers / Q000032:analysis*; D000818:Animals; D001562:Benzimidazoles / Q000032:analysis*; D001565:Benzoates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D000077333:Telmisartan; D014020:Tissue Extracts / Q000737:chemistry*
D047228:Angiotensin II Type 1 Receptor Blockers; D001562:Benzimidazoles; D001565:Benzoates; D014020:Tissue Extracts; D000077333:Telmisartan