The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription.
The WSTF (Williams syndrome transcription factor) protein is involved in vitamin D-mediated transcription and replication as a component of two distinct ATP-dependent chromatin remodeling complexes, WINAC and WICH, respectively. We show here that the WICH complex (WSTF-SNF2h) interacts with several nuclear proteins as follows: Sf3b155/SAP155, RNA helicase II/Gualpha, Myb-binding protein 1a, CSB, the proto-oncogene Dek, and nuclear myosin 1 in a large 3-MDa assembly, B-WICH, during active transcription. B-WICH also contains RNAs, 45 S rRNA, 5 S rRNA, 7SL RNA, and traces of the U2 small nuclear RNA. The core proteins, WSTF, SNF2h, and nuclear myosin 1, are associated with the RNA polymerase III genes 5 S rRNA genes and 7SL, and post-transcriptional silencing of WSTF reduces the levels of these transcripts. Our results show that a WSTF-SNF2h assembly is involved in RNA polymerase III transcription, and we suggest that WSTF-SNF2h-NM1 forms a platform in transcription while providing chromatin remodeling.
Cavellán
Erica
E
Department of Cell Biology, The Wenner-Gren Institute, Arrhenius Laboratories E5, Stockholm University, SE-106 91 Stockholm, Sweden.
Asp
Patrik
P
Percipalle
Piergiorgio
P
Farrants
Ann-Kristin Ostlund
AK
D000251:Adenosine Triphosphatases / Q000737:chemistry*; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D008954:Models, Biological; D009218:Myosins / Q000737:chemistry; D011485:Protein Binding; D000090063:Proto-Oncogene Mas; D012313:RNA / Q000737:chemistry; D012320:RNA Polymerase III / Q000378:metabolism; D013347:Subcellular Fractions; D014157:Transcription Factors / Q000737:chemistry*; D014158:Transcription, Genetic; D000092468:Bromodomain Containing Proteins
D000251:Adenosine Triphosphatases; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D009218:Myosins; D000090063:Proto-Oncogene Mas; D012313:RNA; D012320:RNA Polymerase III; D014157:Transcription Factors; C116750:BAZ1B protein, human; D000092468:Bromodomain Containing Proteins; C000715470:MAS1 protein, human; C409462:SMARCA5 protein, human