Hepatoprotective compounds of the roots of Cudrania tricuspidata on tacrine-induced cytotoxicity in Hep G2 cells.
The bioassay-guided fractionation of the MeOH extract of the root barks of Cudrania tricuspidata furnished three hepatoprotective compounds, gerontoxanthone A (2), cudraflavone B (4), gericudranin E (5), together with four prenylated xanthones, cudraxanthone B (3), isocudraxanthone K (6), cudraxanthone C (7), cudraxanthone H (8), and a prenylated flavanone, euchrestaflavanone C (1). Compounds 4 and 5 showed significant hepatoprotective effects with the EC50 values of 37.39+/-0.4 and 39.87+/-0.7 microM, respectively, and compound 2 showed moderate hepatoprotective effect with an EC50 value of 125.9+/-1.5 microM, against tacrine-induced cytotoxicity in Hep G2 cells. Silybin as positive control showed an EC50 value of 84.76+/-0.5 microM. Isocudraxanthone K (6) is a new compound.
An
Ren-Bo
RB
College of Pharmacy, Wonkwang University, Iksan, Korea.
Sohn
Dong-Hwan
DH
Kim
Youn-Chul
YC
D045744:Cell Line, Tumor; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D029586:Moraceae / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013619:Tacrine / Q000633:toxicity*
D013619:Tacrine