Achieving rapid low-pressure ion chromatography separations on short silica-based monolithic columns.
Short silica-based monolithic columns (0.5-1 cm) are coated with the surfactant didodecyldimethylammonium bromide (DDAB) and used for fast ion exchange separations of small inorganic anions. Sources of extra-column band broadening are assessed and minimized to obtain separations of seven analytes (iodate, chloride, nitrate, bromide, nitrite, phosphate, sulphate) in two minutes at 2 mL/min. Eluents used are 6 and 9 mM 4-cyanophenol at pH 7.3-7.4 or 5 mM 4-hydroxybenzoic acid at pH 5.6. DDAB coating stability is improved by 15-fold by the addition of a DDAB coated pre-column before the injection valve. Separations are obtained using a low-pressure glass syringe.
Pelletier
Sarah
S
Department of Chemistry, Gunning/Lemieux Chemistry Centre, University of Alberta, Edmonton, Alta., Canada T6G 2G2.
Lucy
Charles A
CA
D000399:Air Pressure; D000837:Anion Exchange Resins / Q000737:chemistry; D000838:Anions / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D007287:Inorganic Chemicals / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D012995:Solubility
D000837:Anion Exchange Resins; D000838:Anions; D007287:Inorganic Chemicals; D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium; D012822:Silicon Dioxide