[Experimental study of T lymphocyte reactivity inhibited by allogeneic bone marrow mesenchymal stem cells].
OBJECTIVE
To address the question whether bone marrow mesenchymal stem cells (MSCs) could lower responsiveness of allogeneic T lymphocytes against alloantigens, and explore a feasible strategy for prevention of graft versus host disease (GVHD) occurred in allogeneic bone marrow transplantation.


METHODS
T cells were co-cultured with (60)Co-irradiated bone marrow MSCs from different individuals. The proliferative activity of T cells and their reactivity to allogeneic cells and ConA were evaluated with (3)H-TdR incorporation assay.


RESULTS
T cells could not be activated upon primary or even secondary exposure to allogeneic MSCs (compared the CPM value of 27,529 +/- 969 of T cell alone with that of primary and secondary exposures to allogeneic MSCs were 9,126 +/- 654 and 13,260 +/- 874, respectively). When MSCs were induced to express HLA-DR, they still could not elicit T cell activation. The proliferation rate of allogenous T cells exposed to MSCs was dramatically declined when T cells from the same donor's MSCs were used as stimulator (CPM value decreased from 45,876 +/- 5285 before coculture to 9850 +/- 1618 after coculture). Furthermore, the results remained unchanged even ConA was added into the culture system.


CONCLUSIONS
Heterogenetic MSCs could suppress T cell activation. MSCs pretreatment might be useful in the prevention of GVHD in HLA-mismatched bone marrow transplantation.
Chen
Jian-lin
JL
Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of Chinese Academy of Military Medical Science, Hematopoietic Stem Cell Transplantation Center of PLA, Beijing 100039, China.
Feng
Kai
K
Guo
Zi-Kuan
ZK
U
Ren-na
RN
Xu
Chen
C
Li
Yu-hang
YH
Liu
Xiao-dan
XD
Mao
Ning
N
Chen
Hu
H
D001854:Bone Marrow Cells / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D006086:Graft vs Host Disease / Q000276:immunology / Q000517:prevention & control; D006801:Humans; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*