Distribution and heterogeneity of small ruminant lentivirus envelope subtypes in naturally infected French sheep.
Small ruminants lentiviruses (SRLV) nucleotide sequences spanning the V1V2 variable regions of the env gene were amplified by nested-PCR from 38 blood samples collected from 16 naturally infected sheep flocks in France. For the rapid SRLV group determination of field isolates, the PCR-amplified fragments were subjected to a SRLV-adapted heteroduplex mobility assay (HMA). All viral sequences were clearly assignable to the SRLV group B by HMA analysis. Twenty-seven SRLV isolates were selected for DNA sequence analysis. In each case, nucleotide comparison and phylogenetic analyses confirmed the genetic relationships inferred by HMA. Six SRLV isolates belonged to subtype B1, and 21 pertained to subtype B2, one flock being infected with both subtypes. Subtypes B1 and B2 were found with different frequencies and geographic spread, but exhibited similar genetic diversities. These results give a more complete picture of the distribution and heterogeneity of SRLV env subtypes in sheep and confirmed that multiple interspecies transmission occurred in the past. Furthermore, HMA appeared to be a rapid and reliable method to differentiate caprine arthritis encephalitis virus from maedi-visna virus.
Germain
Karine
K
Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches Caprines, B.P. 3081, 60 rue de Pied de Fond, F-79012 Niort Cedex, France.
Valas
Stephen
S
D000818:Animals; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics*; D020180:Heteroduplex Analysis; D016086:Lentivirus / Q000145:classification / Q000235:genetics*; D016180:Lentivirus Infections / Q000453:epidemiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology*; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000235:genetics*
D006023:Glycoproteins; D014759:Viral Envelope Proteins