title: Age as a determinant factor for endotoxin induced uveitis.
abstract: OBJECTIVE
  To determine if susceptibility to systemic endotoxin-induced uveitis is an age-related phenomenon in the rabbit.
  
  
  METHODS
  Young and adult rabbits were injected intravenously with 2.5 microg/kg of E. coli endotoxin or saline. Thereafter, the number of exudating cells at 2, 6, 12, 24 and 48 hours were determined. The levels of lipopolysaccharide-binding protein, total protein, prostaglandin-E2, nitric oxide and interleukin-6 in aqueous humor were also determined 24 hours after the injections.
  
  
  RESULTS
  A significant increase in the number of exudating cells and the levels of lipopolysaccharide-binding protein, total protein, prostaglandin-E2 and nitric oxide in aqueous humor was observed only in adult rabbits 24 hours after endotoxin injection. No differences were observed in the increased IL-6 levels.
  
  
  CONCLUSIONS
  Life stage seems to be a critical factor in developing an eye-inflammatory response induced by systemic endotoxin. This could be a consequence of a differential specific activation of the ocular immune response.
authors.affiliation: Cellular and Molecular Pathology Laboratory, Venezuelan Institute for Scientific Research (IVIC), Caracas, Venezuela.
authors.forename: Beatriz E
authors.initials: BE
authors.lastname: Brito
authors.forename: Junedy C
authors.initials: JC
authors.lastname: Marcano
authors.forename: Eva
authors.initials: E
authors.lastname: Salazar
authors.forename: Marisol
authors.initials: M
authors.lastname: Cano
authors.forename: Leslie
authors.initials: L
authors.lastname: Baute
authors.forename: Geraldine
authors.initials: G
authors.lastname: Bernal
authors.forename: Luis R
authors.initials: LR
authors.lastname: Gonzalez
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001082:Aqueous Humor / Q000166:cytology / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D009504:Neutrophils / Q000473:pathology*; D009569:Nitric Oxide / Q000378:metabolism; D011817:Rabbits; D014605:Uveitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*
chemical_list: D015415:Biomarkers; D005136:Eye Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D015232:Dinoprostone