[Effects of soy isoflavone on gene expression of adiponectin in insulin-resistance rats induced by high-fat and high-sugar diet].
OBJECTIVE
To study the effects and mechanisms of soy isoflavone (SIF) on improving insulin resistance (IR) status in IR rats induced by high-fat and high-sugar diet.


METHODS
IR rats induced by high-fat and high-sugar diet were assigned into four groups and gavaged with SIF at doses of 0 (model control group), 50, 150 and 450 mg/kg bw for 4 weeks, respectively. Insulin, glucose, adiponectin in serum and adiponectin mRNA in perirened adipocyte were detected.


RESULTS
Compared with model control group, body weight and fat pad weight were lower, and serum adiponectin and adiponectin mRNA expression were higher in the 450 mg/kg bw group, insulin and insulin-resistance index were lower in the 150 mg/kg bw group and 450 mg/kg bw. There is no differences in plasma glucose levels among groups. There is an negative correlation between insulin-resistance index and serum adiponectin level (Y = 24.802 - 2.856X, r = - 0.881, n = 47, P < 0.01).


CONCLUSIONS
These results suggest that soy isoflavone may decrease adipose deposition, increase serum adiponectin level, and ameliorate insulin sensitivity.
Chen
Shi-Wei
SW
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Zhang
Hong-Min
HM
Zhang
Li-Shi
LS
Feng
Xiao-Fan
XF
Peng
Xiaoli
X
D052242:Adiponectin / Q000097:blood* / Q000235:genetics; D000818:Animals; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004041:Dietary Fats / Q000008:administration & dosage*; D007333:Insulin Resistance; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max / Q000737:chemistry
D052242:Adiponectin; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D007529:Isoflavones; D012333:RNA, Messenger