title: Isolation and characterization of a novel glutathione S-transferase-activating peptide from the oriental medicinal plant Phellodendron amurense.
abstract: The aim of this study was to elucidate the characteristics of glutathione S-transferase (GST)-activating compounds from medicinal plants. Among 265 kinds of medicinal plants, Phellodendron amurense showed the highest GST activity at 174.8%. The GST-activating compound of P. amurense was maximally extracted when treated with distilled water at 30 degrees C for 12 h. The compound was purified by ultrafiltration, Sephadex G-10 gel filtration chromatography, and reverse-phase HPLC. The purified GST-activating compound from P. amurense was a novel tetrapeptide with an amino acid sequence of Ala-Pro-Trp-Cys and its molecular weight was estimated to be 476 Da. It also displayed a clear detoxicative effect in 1-chloro-2,4-dinitrobenzene treated mice at a dosage of mg/kg body weight.
authors.affiliation: Department of Life Science and Genetic Engineering, Paichai University, Daejeon 302-735, Republic of Korea.
authors.forename: Ju Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Dae Hyoung
authors.initials: DH
authors.lastname: Lee
authors.forename: Hyung Eun
authors.initials: HE
authors.lastname: Yu
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Jong Soo
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D004137:Dinitrochlorobenzene / Q000494:pharmacology / Q000633:toxicity; D005982:Glutathione Transferase / Q000378:metabolism*; D007723:Korea; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D032102:Phellodendron / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry* / Q000166:cytology
chemical_list: D004137:Dinitrochlorobenzene; D009842:Oligopeptides; D010936:Plant Extracts; C514606:alanyl-prolyl-tryptophyl-cysteine; D005982:Glutathione Transferase