title: Suppressive effects of young radish cultivated with sulfur on growth and metastasis of B16-F10 melanoma cells.
abstract: The oral administration of extracts of young radishes cultivated with sulfur after intravenous tumor cell injection achieved a marked reduction of pulmonary colonization in mice. Treatment of the mice with extracts of young radish cultivated with sulfur did not show any increase in the number of CD8+ or NK T cells in the spleen, indicating no influence on host immunity. Sulforaphane, which could be a candidate for an active compound from young radishes cultivated with sulfur, inhibited cell growth of B16-F10 melanoma cells. In addition, extracts of the young radish cultivated with sulfur-fed group showed enhanced quinine reductase (QR) activities in the liver and lung and a slight increase of glutathione S-transferase (GST) activity in the liver. These results suggested that the administration of extracts of young radishes cultivated with sulfur suppressed pulmonary tumorigenesis, possibly due to increased activity of detoxification enzymes in the liver and lung, and partly due to cell cytotoxicity.
authors.affiliation: Department of Biological Sciences and Immunomodulation Research Center, University of Ulsan, Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Byung-Sam
authors.initials: BS
authors.lastname: Kim
authors.forename: Tae-Wook
authors.initials: TW
authors.lastname: Kyung
authors.forename: Sang-Chul
authors.initials: SC
authors.lastname: Lee
authors.forename: Chi-Woong
authors.initials: CW
authors.lastname: Rho
authors.forename: Kyung-Rak
authors.initials: KR
authors.lastname: Choi
authors.forename: Hae-Jun
authors.initials: HJ
authors.lastname: Hwang
authors.forename: Hye-Seon
authors.initials: HS
authors.lastname: Choi
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005982:Glutathione Transferase / Q000378:metabolism; D017879:Isothiocyanates; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008168:Lung / Q000187:drug effects / Q000201:enzymology; D008175:Lung Neoplasms / Q000473:pathology / Q000517:prevention & control / Q000556:secondary*; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D031224:Raphanus / Q000737:chemistry; D013454:Sulfoxides; D013455:Sulfur; D013861:Thiocyanates / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017879:Isothiocyanates; D010936:Plant Extracts; D013454:Sulfoxides; D013861:Thiocyanates; D013455:Sulfur; D016660:NAD(P)H Dehydrogenase (Quinone); D005982:Glutathione Transferase; C016766:sulforaphane