Partnering between monomers of cyclooxygenase-2 homodimers.
Prostaglandin endoperoxide H synthases (PGHSs) 1 and 2 convert arachidonic acid to prostaglandin H2 in the committed step of prostanoid biosynthesis. These enzymes are pharmacological targets of nonsteroidal antiinflammatory drugs and cyclooxygenase (COX) 2 inhibitors. Although PGHSs function as homodimers and each monomer has its own COX and peroxidase active sites, the question of whether there is cross-talk between monomers has remained unresolved. Here we describe two heterodimers in which a native subunit of human PGHS-2 has been coupled to a subunit having a defect within the COX active site at some distance from the dimer interface. Native/G533A PGHS-2, a heterodimer with a COX-inactive subunit, had the same specific COX activity as the native homodimer. Native/R120Q PGHS-2, a heterodimer in which both subunits can oxygenate arachidonic acid but in which the R120Q subunit cannot bind the COX inhibitor flurbiprofen, was inhibited by flurbiprofen to about the same extent as native PGHS-2. These results imply that native PGHS-2 exhibits half-of-sites reactivity. Isothermal titration calorimetry established that only one monomer of the native PGHS-2 homodimer binds flurbiprofen tightly. In short, binding of ligand to the COX site of one monomer alters its companion monomer so that it is unable to bind substrate or inhibitor. We conclude that PGHS monomers comprising a dimer, although identical in the resting enzyme, differ from one another during catalysis. The nonfunctioning subunit may provide structural support enabling its partner monomer to catalyze the COX reaction. This subunit complementarity may prove to be characteristic of other dimeric enzymes having tightly associated monomers.
Yuan
Chong
C
Department of Biological Chemistry, University of Michigan Medical School, 5416 Medical Science Building I, 1301 East Catherine Street, Ann Arbor, MI 48109-0606, USA.
Rieke
Caroline Jill
CJ
Rimon
Gilad
G
Wingerd
Byron A
BA
Smith
William L
WL
D016718:Arachidonic Acid / Q000737:chemistry; D001665:Binding Sites; D051546:Cyclooxygenase 2 / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D019281:Dimerization; D005480:Flurbiprofen / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008024:Ligands; D021122:Protein Subunits / Q000737:chemistry / Q000187:drug effects / Q000235:genetics; D013379:Substrate Specificity
D016861:Cyclooxygenase Inhibitors; D008024:Ligands; D021122:Protein Subunits; D016718:Arachidonic Acid; D005480:Flurbiprofen; D051546:Cyclooxygenase 2