Differential gene expression of human CD34+ hematopoietic stem and progenitor cells before and after culture.
Ex vivo expanded CD34+ hematopoietic stem and progenitor cells (HSPCs) have compromised homing and engraftment capacities. To investigate underlying mechanisms for functional changes of expanded HSPCs, we compared gene expression profiling of cultured and fresh CD34+ cells derived from cord blood using SMART-PCR and cDNA array: 20 genes were up-regulated while 25 genes were down-regulated in cultured CD34+ HSPCs. These differentially expressed genes are involved primarily in proliferation, differentiation, apoptosis, and homing.
Li
Qunliang
Q
The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China.
Cai
Haibo
H
Liu
Qiwei
Q
Tan
Wen-Song
WS
D018952:Antigens, CD34; D018929:Cell Culture Techniques / Q000379:methods*; D003114:Colony-Forming Units Assay / Q000379:methods; D005312:Fetal Blood / Q000166:cytology; D015870:Gene Expression / Q000235:genetics*; D020869:Gene Expression Profiling; D006412:Hematopoietic Stem Cells; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction
D018952:Antigens, CD34