Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs.
The aim of this study was to investigate differences in concentrations of vitamin A, transthyretin (TTR) and retinol-binding protein (RBP) between plasma and cerebrospinal fluid (CSF) in dogs. RBP was detected using ELISA, and both RBP and TTR by Western blot analysis after separation on SDS-PAGE. Vitamin A was determined by high performance liquid chromatography. RBP and TTR as well as vitamin A were detected in all samples but at substantially lower concentrations in CSF compared to plasma. RBP in dog plasma showed a similar molecular mass to that of humans, whereas canine TTR had a lower molecular mass. Comparison between plasma and CSF showed that both RBP and TTR were of lower molecular mass in CSF. In CSF, RBP and retinol were present at 10-100-fold lower concentrations compared to plasma. Retinyl esters were present only in minute amounts in 5/17 samples. In conclusion, the CSF of dogs compared to humans is significantly different in terms of both quality and quantity of transport proteins for vitamin A.
Forterre
Simone
S
Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Potsdam-Rehbrücke, Germany. forterre@rz.uni-potsdam.de
Raila
Jens
J
Forterre
Franck
F
Brunnberg
Leo
L
Schweigert
Florian J
FJ
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004285:Dogs / Q000134:cerebrospinal fluid*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006801:Humans; D008970:Molecular Weight; D011228:Prealbumin / Q000032:analysis / Q000134:cerebrospinal fluid* / Q000737:chemistry; D012177:Retinol-Binding Proteins / Q000032:analysis / Q000134:cerebrospinal fluid* / Q000737:chemistry; D054839:Retinol-Binding Proteins, Plasma; D013045:Species Specificity; D014801:Vitamin A / Q000097:blood / Q000134:cerebrospinal fluid*
D011228:Prealbumin; D012177:Retinol-Binding Proteins; D054839:Retinol-Binding Proteins, Plasma; D014801:Vitamin A