title: Invasive potential of bacterial isolates associated with subclinical bovine mastitis.
abstract: This work describes differences in the invasive ability of bacterial isolates associated with mastitis. Invasion ability was determined by the uptake and survival in a primary culture of bovine mammary epithelial cells (BMEC). BMEC were isolated from a healthy lactating cow and characterized by their morphology, immunostaining for cytokeratin and the detection of beta- and kappa-casein mRNAs. Ten bacterial isolates comprising the staphylococcal species Staphylococcus aureus (3), Staphylococcus epidermidis (1), Staphylococcus haemolyticus (1), Staphylococcus equorum (2), Staphylococcus xylosus (1) and Brevibacterium stationis (2) obtained from raw milk of cows with mastitis from backyard farms were assayed for their ability to invade BMEC. Only two S. aureus and one S. epidermidis isolates were able to invade BMEC, at similar levels to the S. aureus control strain ATCC 27543. In conclusion, using the in vitro model of infection used in this study, differences in bacterial invasion capability may be detected.
authors.affiliation: Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Apartado Postal 53, Administración Chapultepec Oriente, C.P. 58262 Morelia, Michoacán, México.
authors.forename: José L
authors.initials: JL
authors.lastname: Anaya-López
authors.forename: Oscar E
authors.initials: OE
authors.lastname: Contreras-Guzmán
authors.forename: Alfonso
authors.initials: A
authors.lastname: Cárabez-Trejo
authors.forename: Victor M
authors.initials: VM
authors.lastname: Baizabal-Aguirre
authors.forename: Joel E
authors.initials: JE
authors.lastname: López-Meza
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Valdez-Alarcón
authors.forename: Alejandra
authors.initials: A
authors.lastname: Ochoa-Zarzosa
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000382:microbiology*; D005260:Female; D008321:Mammary Glands, Animal / Q000166:cytology / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013210:Staphylococcus / Q000302:isolation & purification* / Q000502:physiology