title: [Production and identification of monoclonal antibody against N terminal of AIB1].
abstract: OBJECTIVE
  To make and identify the monoclonal antibody against AIB1-N.
  
  
  METHODS
  BALB/c mice were immunized with purified GST-AIB1-N protein, McAb against AIB1-N was produced by hybridoma technique. ELISA and Western-blot were used to identify the immunoglobin subtype and specificity. Results A hybridoma cell was successfully produced to secrete the McAb against AIB1-N, which was identified to belong in IgG1 subtype. By western-blot, the McAb against AIB1 displayed strongly specificity and high affinity.
  
  
  CONCLUSIONS
  The McAb against AIB1 protein may be a useful tool for studying the biological properties of AIB1 expression and the clinical laboratory detection.
authors.affiliation: West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Qiu-Xia
authors.initials: QX
authors.lastname: Pang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Peng
authors.forename: Ming-Rong
authors.initials: MR
authors.lastname: Xi
authors.forename: Yue-Ming
authors.initials: YM
authors.lastname: Zhu
authors.forename: Xu-Hong
authors.initials: XH
authors.lastname: Peng
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Qu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000912:Antibodies, Neoplasm / Q000096:biosynthesis / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology*; D005260:Female; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D056921:Nuclear Receptor Coactivator 3; D014157:Transcription Factors / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D014157:Transcription Factors; D056921:Nuclear Receptor Coactivator 3