Review of successful treatment for Helicobacter species in laboratory mice.
Three variations of the amoxycillin-based triple therapy (amoxycillin, metronidazole and bismuth) were administered in the diet, by oral gavage or in the diet in conjunction with cross-fostering on to Helicobacter-free foster mothers to mice naturally infected with H. hepaticus and/or H. bilis. The presence of Helicobacter species was determined by polymerase chain reaction (PCR) analysis of faecal pellets. Helicobacter infection was eliminated in 50% of strains of mice treated by oral gavage; 57% of strains of mice treated by medicated diet alone and 100% of strains of mice treated with the medicated diet in conjunction with cross-fostering on to Helicobacter-free foster mothers. Eight strains of mice were successfully treated for Helicobacter infection over a two-year period. The mouse colony has been maintained Helicobacter free, as determined by PCR analysis and has remained off treatment from December 2002 to March 2005.
Kerton
Angela
A
Central Biomedical Services, Imperial College, London, UK. a.kerton@imperial.ac.uk
Warden
Paul
P
D000284:Administration, Oral; D000658:Amoxicillin / Q000008:administration & dosage / Q000627:therapeutic use; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D001729:Bismuth / Q000008:administration & dosage / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004359:Drug Therapy, Combination; D005243:Feces / Q000382:microbiology; D005260:Female; D016998:Helicobacter / Q000235:genetics / Q000254:growth & development*; D016481:Helicobacter Infections / Q000188:drug therapy* / Q000382:microbiology / Q000662:veterinary*; D008795:Metronidazole / Q000008:administration & dosage / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D012376:Rodent Diseases / Q000188:drug therapy* / Q000382:microbiology*
D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D008795:Metronidazole; D000658:Amoxicillin; D001729:Bismuth