Immunological basis for IgE hyper-production in enfuvirtide-treated HIV- positive patients.
We previously reported that enfuvirtide (ENF) treatment is accompanied by a selective increase of serum IgE. We asked whether ENF had intrinsic capability to direct B-lymphocytes to switch to IgE and/or if it could drive CD4 T cells to a Th2 phenotype. ENF was added in vitro: (a) to B-lymphocytes stimulated with IgE-switch inducing stimuli; (b) to peripheral blood mononuclear cells. Total IgE production by B cells and IL4 and IFN-gamma production by CD4 T lymphocytes were evaluated, respectively. ENF had no measurable effect on the IgE production by B-lymphocytes. In contrast, it sharply increased the IL4 to IFN-gamma (a correlate of the Th2 phenotype) when added in vitro to T cells from healthy donors or from single ENF-treated patients. The hyper-IgE production in ENF-treated patients is associated with the in vitro induction of a type-2 phenotype in CD4 T cells.
Burastero
Samuele E
SE
Unit of Leukocyte Biology, San Raffaele Scientific Institute, Milan, Italy. s.burastero@hsr.it
Paolucci
Clara
C
Breda
Daniela
D
Soldarini
Armando
A
Dorigatti
Fernanda
F
Soprana
Elisa
E
Hasson
Hamid
H
Biswas
Priscilla
P
Lazzarin
Adriano
A
Castagna
Antonella
A
D019380:Anti-HIV Agents / Q000627:therapeutic use*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D000077560:Enfuvirtide; D015700:HIV Envelope Protein gp41 / Q000627:therapeutic use*; D015658:HIV Infections / Q000097:blood / Q000188:drug therapy* / Q000276:immunology*; D006801:Humans; D017578:Immunoglobulin Class Switching / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000276:immunology; D010446:Peptide Fragments / Q000627:therapeutic use*; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology
D019380:Anti-HIV Agents; D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments; D000077560:Enfuvirtide; D007073:Immunoglobulin E