AlOH3-adjuvanted vaccine-induced macrophagic myofasciitis in rats is influenced by the genetic background.
Macrophagic myofasciitis (MMF) is a specific histopathologic lesion involved in the persistence for years of aluminum hydroxide [Al(OH)(3)] at the site of previous intramuscular (i.m.) injection. In order to study mechanisms involved persistence of MMF lesions, we set up an experimental model of MMF-lesion in Sprague-Dawley and Lewis rat, by i.m. injections of 10 microL of an Al(OH)(3)-adjuvanted vaccine. An evaluation carried out over a 12-month period disclosed significant shrinkage of MMF lesions with time. A radioisotopic study did not show significant aluminium uptake by Al(OH)(3)-loaded macrophages. A morphometric approach showed that Lewis rats with Th1-biased immunity had significantly smaller lesions than Sprague-Dawley rats with balanced Th1/Th2 immunity. Concluding, our results indicate that genetic determinatives of cytotoxic T-cell responses could interfere with the clearance process and condition the persistence of vaccine-induced MMF-lesions.
Authier
François-Jérôme
FJ
Centre de Référence Pour Maladies Neuromusculaires, CHU Henri Mondor, AP-HP, Créteil, France. francois-jerome.authier@hmn.aphp.fr
Sauvat
Stéphane
S
Christov
Christo
C
Chariot
Patrick
P
Raisbeck
Grant
G
Poron
Marie-Françoise
MF
Yiou
Françoise
F
Gherardi
Romain
R
D000536:Aluminum Hydroxide / Q000009:adverse effects*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D007111:Immunity, Cellular / Q000187:drug effects / Q000276:immunology; D007273:Injections, Intramuscular / Q000009:adverse effects; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D009209:Myofascial Pain Syndromes / Q000139:chemically induced* / Q000235:genetics* / Q000503:physiopathology; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology; D014612:Vaccines / Q000009:adverse effects*
D014612:Vaccines; D000536:Aluminum Hydroxide