title: Differential glycosylation of rhLf expressed in the mammary gland of transgenic mice.
abstract: Differential glycosylation of natural hLf and rhLf from hLf-transgenic mice, which harbored a 146 Kb BAC insert that includes the intact hLf gene sequence, was studied in the present report. There were significant differences between the immunoblotting results of rhLf and natural hLf, which were denatured with nonreducing SDS sample buffer. The differences disappeared after rhLf and natural hLf samples were digested with N-glycosidase F, respectively. The results showed that there were significant differences (P<0.01) between the glycosylation of natural hLf and rhLf that were purified, respectively, from milk samples of seven hLf-transgenic mouse lines.
authors.affiliation: Key Laboratories of Ministry of Agriculture of P.R. China for Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University, Beijing, P.R. China.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Zhao
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Liu
authors.forename: Baoliang
authors.initials: B
authors.lastname: Fan
authors.forename: Yunping
authors.initials: Y
authors.lastname: Dai
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.forename: Meili
authors.initials: M
authors.lastname: Wang
authors.forename: Huiling
authors.initials: H
authors.lastname: Niu
authors.forename: Fengong
authors.initials: F
authors.lastname: Xi
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Dinglian
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006031:Glycosylation; D006801:Humans; D015151:Immunoblotting; D007781:Lactoferrin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008892:Milk / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D007781:Lactoferrin; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase