Complete assignment of the chromosomes of Gossypium hirsutum L. by translocation and fluorescence in situ hybridization mapping.
Significant progress has been made in the construction of genetic maps in the tetraploid cotton Gossypium hirsutum. However, six linkage groups (LGs) have still not been assigned to specific chromosomes, which is a hindrance for integrated genetic map construction. In the present research, specific bacterial artificial chromosome (BAC) clones constructed in G. hirsutum acc. TM-1 for these six LGs were identified by screening the BAC library using linkage group-specific simple-sequence repeats markers. These BAC clones were hybridized to ten translocation heterozygotes of G. hirsutum. L as BAC-fluorescence in situ hybridization probes, which allowed us to assign these six LGs A01, A02, A03, D02, D03, and D08 to chromosomes 13, 8, 11, 21, 24, and 19, respectively. Therefore, the 13 homeologous chromosome pairs have been established, and we have proposed a new chromosome nomenclature for tetraploid cotton.
Wang
Kai
K
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. cotton@njau.edu.cn
Song
Xianliang
X
Han
Zhiguo
Z
Guo
Wangzhen
W
Yu
John Z
JZ
Sun
Jing
J
Pan
Jiaju
J
Kohel
Russell J
RJ
Zhang
Tianzhen
T
D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D032461:Chromosomes, Plant / Q000145:classification / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D003368:Gossypium / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D018598:Minisatellite Repeats; D011123:Polyploidy; D009626:Terminology as Topic; D014178:Translocation, Genetic
D018744:DNA, Plant; D005819:Genetic Markers