Green fluorescent protein is a reliable reporter for screening signal peptides functional in Lactobacillus reuteri.
A signal peptide (SP)-probe vector pNICE-gfpSP, which employed a green fluorescent protein (Gfp) as the SP-selection marker, was constructed for use in Lactobacillus reuteri. This chimerical plasmid allowed cloning and screening DNA fragments with the SP function by direct visualization of the expressed fluorescence activity around cells. Assay of fluorescent intensity in their culture supernatant with spectrofluorometry further enabled quantifying the secretion efficiency of the identified SP fragment.
Wu
Chi-Ming
CM
Institute of Veterinary Microbiology, National Chung-Hsing University, Taichung, Taiwan, ROC.
Chung
Tung-Ching
TC
D000595:Amino Acid Sequence; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics*; D052200:Limosilactobacillus reuteri / Q000235:genetics*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics*
D021382:Protein Sorting Signals; D049452:Green Fluorescent Proteins