title: Effects of salinity levels on proteome of Suaeda aegyptiaca leaves.
abstract: Saline soils are the major problem of cultivated lands of Iran. Suaeda aegyptiaca is a salt-tolerant plant (halophytes) that grow naturally in salt-affected areas of Iran. We have employed proteomics to identify the mechanisms of salt responsiveness in leaves of S. aegyptiaca grown under different salt concentrations. Ten-day-old plants were treated with 0, 150, 300, 450, and 600 mM NaCl. After 30 days of treatment, leaf samples were collected and analyzed using 2-D-PAGE. Out of 700 protein spots reproducible detected within replications, 102 spots showed significant response to salt treatment compared to 0 mM NaCl. We analyzed expression pattern of salt-responsive proteins using a hierarchical and two nonhierarchical (Fuzzy ART and SOM) statistical methods and concluded that Fuzzy ART is the superior method. Forty proteins of 12 different expression groups were analyzed using LC/MS/MS. Of these, 27 protein spots were identified including proteins involved in oxidative stress tolerance, glycinebetain synthesis, cytoskeleton remodeling, photosynthesis, ATP production, protein degradation, cyanide detoxification, and chaperone activities. The expression pattern of these proteins and their possible roles in the adaptation of S. aegyptiaca to salinity is discussed.
authors.affiliation: Agricultural Biotechnology Research Institute of Iran, Karaj, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Askari
authors.forename: Johan
authors.initials: J
authors.lastname: Edqvist
authors.forename: Mohsen
authors.initials: M
authors.lastname: Hajheidari
authors.forename: Mohammad
authors.initials: M
authors.lastname: Kafi
authors.forename: Ghasem Hosseini
authors.initials: GH
authors.lastname: Salekdeh
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D019663:Chenopodiaceae / Q000378:metabolism*; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D018384:Oxidative Stress; D010788:Photosynthesis; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry*; D010944:Plants / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012492:Salts / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012492:Salts; D000255:Adenosine Triphosphate