Cloning and expression of a GST-pi gene in Mytilus galloprovincialis. Attempt to use the GST-pi transcript as a biomarker of pollution.
Glutathione S-transferase is involved in the detoxication of many chemical compounds. Northern blot analysis of mRNA GST gene from the mussel Mytilus galloprovincialis shows the presence of a transcript of 850 bp (GenBank accession no. Gi:22094808 and AF527010). The cDNA cloned sequence is constituted by an ORF of 621 bp encoding for a protein of 23,700 Da present in the gills and digestive gland of M galloprovincialis. The sequence, called Mg (M. galloprovincialis) GST-p, is clearly related to the pi class GST. M. galloprovincialis treated with Cd (200 microg/L) and BaP (100 microg/L) or co-treated with Cd and BaP take up cadmium in the gills (16.2 +/- 4.2 and 12.6 +/- 1.2 microg Cd/g dry wt. after exposure to Cd and Cd + BaP, respectively) and in the digestive gland. The transcription of GST-pi gene, by semi-quantitative RT-PCR, shows the lowest value in the digestive glands of mussels exposed to BaP, whereas the treatment with cadmium and the co-treatment with cadmium and BaP evoke GST-pi gene expression higher than controls. Mussels collected from six sites along the south coast of Portugal show different GST-pi transcription levels, some of which are related to their pollutant content.
Hoarau
Pascal
P
Laboratory of Ecotoxicology and Environmental Chemistry, CIMA, FCMA, University of Algarve, Campus de Gambelas, Faro, Portugal.
Damiens
Gautier
G
Roméo
Michèle
M
Gnassia-Barelli
Mauricette
M
Bebianno
Maria J
MJ
D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis / Q000633:toxicity; D015415:Biomarkers / Q000378:metabolism; D002104:Cadmium / Q000032:analysis / Q000633:toxicity; D003001:Cloning, Molecular; D004064:Digestive System / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods; D015971:Gene Expression Regulation, Enzymologic; D005880:Gills / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D051549:Glutathione S-Transferase pi / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D049878:Mytilus / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D010802:Phylogeny; D011174:Portugal; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity
D015415:Biomarkers; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D002104:Cadmium; D001564:Benzo(a)pyrene; D051549:Glutathione S-Transferase pi