title: Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099.
abstract: This study reports the production of xylanolytic and cellulolytic enzymes by a thermophilic fungal isolate Myceliophthora sp. using a cheap medium containing rice straw and chemically defined basal medium under solid-state culture. A combination of one factor at a time approach followed by response surface methodology using Box-Behnken design of experiments resulted in 2.5, 1.25, 1.28 and 4.23 fold increase in xylanase, endoglucanase, beta-glucosidase and FPase activity, respectively. The zymograms developed against IEF gels showed that multiple isoforms of xylanase (5), endoglucanase (4) and beta-glucosidase (2) were produced under optimized culture conditions. Moreover, thiol containing serine proteases produced during the growth of the culture had no role in the post-translational modification of these xylanases.
authors.affiliation: Department of Microbiology, Guru Nanak Dev University, Amritsar 143 005, Punjab, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Badhan
authors.forename: B S
authors.initials: BS
authors.lastname: Chadha
authors.forename: Jatinder
authors.initials: J
authors.lastname: Kaur
authors.forename: H S
authors.initials: HS
authors.lastname: Saini
authors.forename: M K
authors.initials: MK
authors.lastname: Bhat
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D002480:Cellulase / Q000378:metabolism*; D003470:Culture Media; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D006358:Hot Temperature; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D003470:Culture Media; D001617:beta-Glucosidase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases