title: 8-(Pyren-1-yl)-2'-deoxyguanosine as an optical probe for DNA hybridization and for charge transfer with small peptides.
abstract: 8-(Pyren-1-yl)-2'-deoxyguanosine (Py-G) was incorporated synthetically as an optical probe into oligonucleotides. The Py-G group in DNA does not discriminate between any of the four natural nucleosides as a counterbase and exhibits altered optical properties in single strands versus double strands. Thus, the duplex hybridization of Py-G-modified DNA can be observed by both fluorescence and absorption spectroscopy. Moreover, Py-G in DNA can be applied as photoinducable donor for charge transfer processes with small peptides.
authors.affiliation: University of Regensburg, Institute for Organic Chemistry, D-93040 Regensburg, Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Valis
authors.forename: Elke
authors.initials: E
authors.lastname: Mayer-Enthart
authors.forename: Hans-Achim
authors.initials: HA
authors.lastname: Wagenknecht
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes / Q000032:analysis* / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D013057:Spectrum Analysis; D017186:Titrimetry; D044366:Transition Temperature
chemical_list: C512289:8-(pyren-1-yl)-2'-deoxyguanosine; D015341:Nucleic Acid Probes; D010455:Peptides; D011721:Pyrenes; D004247:DNA; D003849:Deoxyguanosine