Induction of interleukin-8 expression in porcine peripheral blood mononuclear cells by trans10-cis12 conjugated linoleic acid.
The chemotaxis of porcine peripheral blood polymorphonuclear cells (PMN) was enhanced by culture supernatant from peripheral blood mononuclear cells (PBMC) treated with trans10-cis12 (10t-12c) conjugated linoleic acid (CLA) or by porcine recombinant (pr) interleukin (IL)-8. To identify this chemotactic inducing factor, the culture supernatant from 10t-12c CLA treated PBMC was partially purified by gel filtration. Only fraction 9 contained enhanced PMN chemotactic activity. The chemotactic activity of fraction 9 was inhibited by an anti-prIL-8 polyclonal antibody (pAb). Porcine IL-8 mRNA expression analyzed by a reverse transcriptase-polymerase chain reaction (RT-PCR) assay was increased in PBMC by the addition of 10t-12c CLA. This study strongly suggests that the immunoenhancing effect of CLA on the chemotactic response of porcine PMN is mediated through IL-8 produced by CLA treated PBMC.
Son
Sung-Mok
SM
Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Kang
Ji-Houn
JH
Lee
Geun-Shik
GS
Jeung
Eui-Bae
EB
Yang
Mhan-Pyo
MP
D000818:Animals; D002633:Chemotaxis / Q000276:immunology; D002850:Chromatography, Gel; D003470:Culture Media; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000097:blood / Q000276:immunology*
D003470:Culture Media; D016209:Interleukin-8; D044243:Linoleic Acids, Conjugated; D012333:RNA, Messenger