title: [Outsourced real-time PCR diagnosis of cutaneous leishmaniasis in the outbreak region of Constatine, Algeria].
abstract: Taking into account the re-emergence of leishmaniasis in the world, the geographic variability of its epidemiology and the growing numbers of travellers, a pilot study on the diagnosis of cutaneous leishmaniasis was undertaken in Constatine, one of the outbreak regions in eastern Algeria. A total of 143 specimens were collected on blotters and tested by real-time PCR. Results were compared with those of direct examination. Diagnosis was positive for leishmaniasis in 81% of cases using PCR versus 48% of cases using microscopy. Real-time PCR showed a significant quantitative difference between patients for whom microscopic diagnosis was positive and those for whom direct examination was negative. The results presented in this study demonstrated the effectiveness and sensitivity of PCR in the diagnosis of cutaneous leishmaniasis from blotter specimens. This technique enabled in-field collection of specimens from each patient and provided prompt results. North-South cooperation based on the use of simple means for transmission of specimens for molecular diagnosis allowed creation of an effective partnership for daily diagnosis and promoted exchange between investigators in preparation for technology transfer.
authors.affiliation: Laboratoire de Parasitologie et Mycologie du CHU Ben Badis, Constantine, Algérie. mihoubii@yahoo.fr
authors.forename: I
authors.initials: I
authors.lastname: Mihoubi
authors.forename: F
authors.initials: F
authors.lastname: de Monbrison
authors.forename: N
authors.initials: N
authors.lastname: Romeuf
authors.forename: T
authors.initials: T
authors.lastname: Moulahem
authors.forename: S
authors.initials: S
authors.lastname: Picot
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000462:Algeria / Q000453:epidemiology; D000818:Animals; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000032:analysis; D005260:Female; D006801:Humans; D007223:Infant; D007891:Leishmania / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D016054:DNA, Protozoan