title: Cytoplasmic Listeria monocytogenes stimulates IFN-beta synthesis without requiring the adapter protein MAVS.
abstract: The mitochondria-associated adapter protein MAVS (also called IPS-1, VISA or CARDIF, designated MAVS for reasons of simplicity in our manuscript) relays signals from cytoplasmic sensors of viral RNA to the IRF3 kinase complex and the interferon-beta (IFN-beta) gene. Using siRNA-mediated knock-down in macrophages we show that IFN-beta synthesis in response to transfected, intracellular double-stranded RNA (dsRNA), a pathogen-associated molecular pattern of viruses, is decreased in absence of MAVS. By contrast, the Gram-positive bacterium Listeria monocytogenes targets the IFN-beta gene without detectable MAVS requirement. The data show that MAVS is not a central adapter protein for all cytoplasmic pathogen sensors that stimulate IFN-beta synthesis.
authors.affiliation: Max F. Perutz Laboratories, University of Vienna, Austria.
authors.forename: Didier
authors.initials: D
authors.lastname: Soulat
authors.forename: Angela
authors.initials: A
authors.lastname: Bauch
authors.forename: Silvia
authors.initials: S
authors.lastname: Stockinger
authors.forename: Giulio
authors.initials: G
authors.lastname: Superti-Furga
authors.forename: Thomas
authors.initials: T
authors.lastname: Decker
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000276:immunology*; D000818:Animals; D002460:Cell Line; D003593:Cytoplasm / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D012330:RNA, Double-Stranded / Q000235:genetics / Q000276:immunology; D034741:RNA, Small Interfering / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D016899:Interferon-beta; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; C512612:MAVS protein, mouse