Mapping novel traits by array-assisted bulk segregant analysis in Saccharomyces cerevisiae.
We demonstrate a new method, microarray-assisted bulk segregant analysis, for mapping traits in yeast by genotyping pooled segregants. We apply a probabilistic model to the progeny of a single cross and as little as two microarray hybridizations to reliably map an auxotrophic marker, a Mendelian trait, and a major-effect quantitative trait locus.
Brauer
Matthew J
MJ
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
Christianson
Cheryl M
CM
Pai
Dave A
DA
Dunham
Maitreya J
MJ
D002874:Chromosome Mapping / Q000379:methods*; D020090:Chromosome Segregation; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011336:Probability; D040641:Quantitative Trait Loci; D012441:Saccharomyces cerevisiae / Q000235:genetics*