Specific phospholipid recognition by human immunodeficiency virus type-1 neutralizing anti-gp41 2F5 antibody.
HIV-1 neutralizing monoclonal antibody (Mab) 2F5 recognizes a membrane-partitioning gp41 sequence. Just recently its capacity to react with cardiolipin has been demonstrated. Here, we have studied the specificity of Mab2F5-phospholipid interactions comparing partitioning into lipid bilayers with recognition of molecular species dispersed in solution. Using a liposome-based ELISA we demonstrate a preferential association with cardiolipin bilayers. When different soluble lysoderivatives were compared in their capacity to inhibit Mab2F5 binding to immobilized HIV-1 peptide epitope, only dilysocardiolipin resulted effective in blocking the process. Dilyso-cardiolipin also competed with native-functional gp41 for 2F5 recognition. Thus, our data support specific cardiolipin recognition by 2F5 that is not dependent on lipid bilayer assembly and involves the epitope-binding site. These findings might be of relevance for understanding the molecular basis of HIV-1 immune evasion.
Sánchez-Martínez
Silvia
S
Biofisika Unitatea (CSIC-UPV/EHU) and Biokimika Saila, Euskal Herriko Unibertsitatea, Posta Kutxa 644, 48080 Bilbao, Spain.
Lorizate
Maier
M
Hermann
Katinger
K
Kunert
Renate
R
Basañez
Gorka
G
Nieva
José L
JL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology; D002308:Cardiolipins / Q000737:chemistry / Q000276:immunology*; D018604:Epitope Mapping / Q000379:methods; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000737:chemistry / Q000276:immunology*; D015497:HIV-1 / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry / Q000276:immunology*; D008561:Membrane Fusion / Q000276:immunology; D010455:Peptides / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D002308:Cardiolipins; D000939:Epitopes; D015700:HIV Envelope Protein gp41; D008051:Lipid Bilayers; D010455:Peptides