Quantitative proteome analysis of breast cancer cell lines using 18O-labeling and an accurate mass and time tag strategy.
Proteome comparison of cell lines derived from cancer and normal breast epithelium provide opportunities to identify differentially expressed proteins and pathways associated with specific phenotypes. We employed 16O/18O peptide labeling, FT-ICR MS, and an accurate mass and time (AMT) tag strategy to simultaneously compare the relative abundance of hundreds of proteins in non-cancer and cancer cell lines derived from breast tissue. A cell line reference panel allowed relative protein abundance comparisons among multiple cell lines and across multiple experiments. A peptide database generated from multidimensional LC separations and MS/MS analysis was used for subsequent AMT tag-based peptide identifications. This peptide database represented a total of 2299 proteins, including 514 that were quantified in five cell lines using the AMT tag and 16O/18O strategies. Eighty-six proteins showed at least a threefold protein abundance change between cancer and non-cancer cell lines. Hierarchical clustering of protein abundance ratios revealed that several groups of proteins were differentially expressed between the cancer cell lines.
Patwardhan
Anil J
AJ
UCSF Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Strittmatter
Eric F
EF
Camp
David G
DG
Smith
Richard D
RD
Pallavicini
Maria G
MG
D001943:Breast Neoplasms / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D010103:Oxygen Isotopes; D020543:Proteome; D017550:Spectroscopy, Fourier Transform Infrared
D010103:Oxygen Isotopes; D020543:Proteome