Isolation and characterization of a novel thermophilic Bacillus strain degrading long-chain n-alkanes.
A thermophilic Bacillus strain NG80-2 growing within the temperature range of 45-73 degrees C (optimum at 65 degrees C) was isolated from a deep subterranean oil-reservoir in northern China. The strain was able to utilize crude oil and liquid paraffin as the sole carbon sources for growth, and the growth with crude oil was accompanied by the production of an unknown emulsifying agent. Further examination showed that NG80-2 degraded and utilized only long-chain (C15-C36) n-alkanes, but not short-chain (C8-C14) n-alkanes and those longer than C40. Based on phenotypic and phylogenic analyses, NG80-2 was identified as Geobacillus thermodenitrificans. The strain NG80-2 may be potentially used for oily-waste treatment at elevated temperature, a condition which greatly accelerates the biodegradation rate, and for microbial enhancing oil recovery process.
Wang
Lei
L
TEDA School of Biological Sciences and Biotechnology, Nankai University, 23# Hong Da Street, TEDA, 300457, Tianjin, People's Republic of China.
Tang
Yun
Y
Wang
Shuo
S
Liu
Ru-Lin
RL
Liu
Mu-Zhi
MZ
Zhang
Yan
Y
Liang
Feng-Lai
FL
Feng
Lu
L
D000473:Alkanes / Q000737:chemistry* / Q000378:metabolism*; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008899:Mineral Oil / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism; D010802:Phylogeny; D013997:Time Factors
D000473:Alkanes; D010578:Petroleum; D008899:Mineral Oil