RNA interference by 2',5'-linked nucleic acid duplexes in mammalian cells.
Synthetic small interfering RNA (siRNA) mediated silencing of a specific gene is emerging as a powerful tool for gene regulation. However, their utility is limited for therapeutic applications primarily due to poor stability. The 2',5'-linked oligonucleotides are known to be more stable to nucleolytic degradation than 3',5'-linked oligonucleotides. The 2',5'-linkage is tolerated in the sense strand of the siRNA duplex. However, the 2',5'-linkage is not tolerated in the antisense strand of the siRNA duplex.
Prakash
Thazha P
TP
Department of Medicinal Chemistry, Isis Pharmaceuticals, Inc., Carlsbad, CA 92008, USA. tprakash@isisph.com
Kraynack
Bryan
B
Baker
Brenda F
BF
Swayze
Eric E
EE
Bhat
Balkrishen
B
D000818:Animals; D020029:Base Pairing; D045744:Cell Line, Tumor; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry* / Q000235:genetics*; D051059:PTEN Phosphohydrolase / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism
D009696:Nucleic Acids; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051059:PTEN Phosphohydrolase