title: Steady state fluorescence spectroscopy of the photosystem II core complex.
abstract: Spectroscopic properties within the core complex of photosystem II were investigated by studying the influence of the wavelength of excitation on the fluorescence emission spectrum. At two temperatures, when the core complex of PSII isolated from spinach was excited at six different excitation wavelengths ranging from 436 nm to 520 nm, there is no difference in the maxima of the emission spectra of the core complex, and when the core complex was excited at 480, 489, 495 and 507 nm respectively, fluorescence intensities of maxima decrease with increasing of the absorbance of the beta-carotene molecules at the four excitation wavelengths. The extent of change of the shoulder of the spectra beyond 700 nm depends on the kind of pigment molecule excited. The excitation wavelength can influence the way of energy transfer in the core complex of photosystem II. By Gaussian deconvolution analysis, at least seven groups of chlorophyll a molecules were discovered. They are Chl a(660), Chl a(670), Chl a(680), Chl a(682), Chl a(684), Chl a(687) and Chl a(690).
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069, China. caixiah2003@sina.com
authors.forename: Xia
authors.initials: X
authors.lastname: Cai
authors.forename: Shui-Cai
authors.initials: SC
authors.lastname: Wang
authors.forename: Jun-Fang
authors.initials: JF
authors.lastname: He
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Ju-Fang
authors.initials: JF
authors.lastname: Peng
authors.forename: Ting-Yun
authors.initials: TY
authors.lastname: Kuang
mesh_terms: D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D018724:Spinacia oleracea / Q000378:metabolism*; D013696:Temperature; D019207:beta Carotene / Q000737:chemistry / Q000378:metabolism
chemical_list: D045332:Photosystem II Protein Complex; D019207:beta Carotene; D002734:Chlorophyll