The effect of beta-bungarotoxin, or geniculate ganglion lesion on taste bud development in the chick embryo.
Chick taste bud (gemmal) primordia normally appear on embryonic day (E) 16 and incipient immature, spherical-shaped buds at E17. In ovo injection of beta-bungarotoxin at E12 resulted in a complete absence of taste buds in lower beak and palatal epithelium at developmental ages E17 and E21. However, putative gemmal primordia (solitary clear cells; small, cell groupings) remained, lying adjacent to salivary gland duct openings as seen in normal chick gemmal development. Oral epithelium was immunonegative to neural cell adhesion molecule (NCAM) suggesting gemmal primordia are nerve-independent. Some NCAM immunoreactivity was evident in autonomic ganglion-like cells and nerve fibers in connective tissue. After unilateral geniculate ganglion/otocyst excision on E2.5, at developmental ages E18 and posthatching day 1, approximately 12% of surviving ipsilateral geniculate ganglion cells sustained approximately 54% of the unoperated gemmal counts. After E18, proportional stages of differentiation in surviving developing buds probably reflect their degree of innervation, as well as rate of differentiation. Irrespective of the degree of geniculate ganglion damage, the proportion of surviving buds can be sustained at the same differentiated bud stage as on the unoperated side, or may differentiate to a later bud stage, consistent with the thesis that bud maturation, maintenance, and survival are nerve-dependent.
Ganchrow
Donald
D
Department of Anatomy and Anthropology, Sackler Faculty of Medicine, Tel Aviv University, 69978, Ramat Aviv, Tel-Aviv, Israel. dganchrow@ucsd.edu
Ganchrow
Judith
J
Witt
Martin
M
Arki-Burstyn
Eve
E
D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology*; D002642:Chick Embryo; D005830:Geniculate Ganglion / Q000166:cytology / Q000502:physiology*; D009061:Mouth Mucosa / Q000737:chemistry; D019006:Neural Cell Adhesion Molecules / Q000032:analysis; D013650:Taste Buds / Q000737:chemistry / Q000187:drug effects* / Q000196:embryology*
D002038:Bungarotoxins; D019006:Neural Cell Adhesion Molecules