[Functional expression of adenylyl cyclase and phosphodiesterase in ejaculated human spermatozoa].
OBJECTIVE
To compare the differences of expressions of adenylyl cyclase (AC) and phosphodiesterase (PDE) in ejaculated spermatozoa between healthy volunteers and the patients with asthenospermia.


METHODS
Ejaculated spermatozoa were collected from healthy volunteers and the patients with asthenospermia. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect mRNA expression of AC and PDE subtypes in human spermatozoa. The concentrations of cAMP and cGMP in the samples were detected by enzyme-linked immunosorbent assay (ELISA).


RESULTS
Compared with healthy volunteers, expression of sAC mRNA and concentration of cAMP were significantly decreased in the patients with asthenospermia (P < 0.01) , while the expression of PDE4C mRNA was significantly increased at the same time (P <0.01). There were no marked differences in the expression of ACIII mRNA and concentration of cGMP between the two groups.


CONCLUSIONS
The sAC down-regulation and PDE4C up-regulation are possible reasons for asthenospermia.
Cai
Zhi-ming
ZM
The Center for Reproductive Medicine, Peking University Shenzhen Hospital, Guangdong, China.
Gui
Yao-ting
YT
Guo
Lian-dian
LD
Zhang
Li-bing
LB
Zhang
Jian-rong
JR
Wang
He
H
Yu
Jie
J
D000262:Adenylyl Cyclases / Q000096:biosynthesis*; D053627:Asthenozoospermia / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism*
D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases