[Expression of human LNalpha4LG4-5 in Pichia yeast].
To carry out the secretive expression of human laminin alpha4 chain LG4-5 module (hLNalpha4LG4-5), recombinant expression plasmid pPICZalphaA-LG45 was constructed by inserting of hLNalpha4LG4-5 cDNA into yeast expression vector pPICZaA. The hLNalpha4LG4-5 protein was expressed in GS115 Pichia yeast strain after induced by methanol, and purified target protein can obviously promote the expansion and adhesion of 293 cells.
Lian
Ji-Qin
JQ
Faculty of Basic Medical Science, Third Military Medical University, Chongqing 400038, China.
Dai
Xu-Fang
XF
Li
Xiao-Hui
XH
He
Feng-Tian
FT
D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D007797:Laminin / Q000096:biosynthesis* / Q000235:genetics; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*
C501369:LAMA4 protein, human; D007797:Laminin; D011993:Recombinant Fusion Proteins