Fanciful FRET.
The validity of experiments based on Förster resonance energy transfer (FRET), an imaging technique widely used to measure protein-protein interactions in living cells, critically depends on the accurate and precise measurement of FRET efficiency. The use of FRET standards to determine FRET efficiency, and a consideration of such factors as how the abundance of FRET acceptors and the stoichiometry of donors and acceptors in a molecular complex can affect measured FRET efficiency, will enhance the usefulness with which FRET experiments can be interpreted.
Vogel
Steven S
SS
National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Laboratory of Molecular Physiology, 5625 Fishers Lane, Bethesda, MD 20892, USA. stevevog@mail.nih.gov
Thaler
Christopher
C
Koushik
Srinagesh V
SV
D000465:Algorithms; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry; D015706:Bibliometrics; D004574:Electromagnetic Fields; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods / Q000592:standards / Q000639:trends; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D005470:Fluorometry / Q000379:methods; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D038761:Photobleaching; D025941:Protein Interaction Mapping / Q000379:methods*; D039781:PubMed / Q000706:statistics & numerical data; D012015:Reference Standards; D012107:Research Design; D020798:Two-Hybrid System Techniques
D001426:Bacterial Proteins; C485182:Cyan Fluorescent Protein; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins