EJC-independent degradation of nonsense immunoglobulin-mu mRNA depends on 3' UTR length.
Inconsistent with prevailing models for nonsense-mediated mRNA decay (NMD) in mammals, the mRNA levels of immunoglobulin-mu (Ig-mu) genes with premature termination codons (PTCs) in the penultimate exon are still reduced by NMD when the intron furthest downstream is deleted. As in yeast, this exon junction complex-independent NMD of Ig-mu mRNAs depends on the distance between the termination codon and the poly(A) tail and suggests an evolutionarily conserved mode of PTC recognition.
Bühler
Marc
M
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH-3012 Bern, Switzerland.
Steiner
Silvia
S
Mohn
Fabio
F
Paillusson
Alexandra
A
Mühlemann
Oliver
O
D020413:3' Untranslated Regions / Q000235:genetics* / Q000378:metabolism*; D018389:Codon, Nonsense / Q000235:genetics*; D039601:Eukaryotic Initiation Factor-4A / Q000378:metabolism; D005091:Exons / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D020871:RNA Stability / Q000235:genetics*
D020413:3' Untranslated Regions; D018389:Codon, Nonsense; D007148:Immunoglobulin mu-Chains; D011061:Poly A; D039601:Eukaryotic Initiation Factor-4A