title: RECS1 deficiency in mice induces susceptibility to cystic medial degeneration.
abstract: RECS1 is a novel shear stress-responsive gene that encodes a protein putatively forming seven-span transmembrane domains. We reports here that mouse RECS1 (mRECS1) transcripts is detected in most tissues except for thymus, spleen and testis. The putative cytoplasmic N-terminus of mRECS1 has a high content of proline (23%) and glycine (12%) residues, contains one PPXY motif, multiple PXXP motifs and one overlapping P(T/S)AP and PPXY motif (P(T/S)APPXY). The PPXY motif lies within one potential PEST sequence (PEST score: +7.65). We prepared anti-RECS1 polyclonal antibody and found by western blot analysis that the mRECS1 protein in the lung and aorta was detected as a 34.4 kDa band. However, one shifted 58 kDa band or three shifted bands (48, 69, 82 kDa) were detected in the heart or the liver, respectively. Since northern blot detected only one species of mRECS1 mRNA in heart and liver tissues, as well as other tissues (approximately 2.2 kb), these differences in molecular weight seem to be due to posttranslational modification. Biochemical fractionation and RECS1-GFP fusion protein revealed that RECS1 localizes at the endosomal/lysosomal membranes in the cytoplasm. To understand the function of RECS1 in the body, we made RECS1 knockout (KO) mice and found that RECS1 KO mice (older than 14 months) are prone to cystic medial degeneration (CMD). Taken together, we conclude that RECS1 is an endosomal/lysosomal membrane protein which plays protective roles in vascular remodeling.
authors.affiliation: Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Suita, Japan.
authors.forename: Hanjun
authors.initials: H
authors.lastname: Zhao
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ito
authors.forename: Shinya H
authors.initials: SH
authors.lastname: Kimura
authors.forename: Norikazu
authors.initials: N
authors.lastname: Yabuta
authors.forename: Naohiko
authors.initials: N
authors.lastname: Sakai
authors.forename: Masahito
authors.initials: M
authors.lastname: Ikawa
authors.forename: Masaru
authors.initials: M
authors.lastname: Okabe
authors.forename: Yuji
authors.initials: Y
authors.lastname: Matsuzawa
authors.forename: Shizuya
authors.initials: S
authors.lastname: Yamashita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nojima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001014:Aortic Aneurysm / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D045744:Cell Line, Tumor; D011992:Endosomes / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D008565:Membrane Proteins / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology*; D013314:Stress, Mechanical
chemical_list: D008565:Membrane Proteins; C523829:RECS1 protein, mouse