Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for biotechnological production of organic acids and amino acids.
Industrial microorganisms have been developed as biocatalysts to provide new or to optimize existing processes for the biotechnological production of chemicals from renewable plant biomass. Rational strain development by metabolic engineering is crucial to successful processes, and is based on efficient genetic tools and detailed knowledge of metabolic pathways and their regulation. This review summarizes recent advances in metabolic engineering of the industrial model bacteria Escherichia coli and Corynebacterium glutamicum that led to efficient recombinant biocatalysts for the production of acetate, pyruvate, ethanol, d- and l-lactate, succinate, l-lysine and l-serine.
Wendisch
Volker F
VF
Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Münster, 48149 Münster, Germany. wendisch@uni-muenster.de
Bott
Michael
M
Eikmanns
Bernhard J
BJ
D000596:Amino Acids / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D009930:Organic Chemicals / Q000378:metabolism*; D011995:Recombination, Genetic
D000596:Amino Acids; D009930:Organic Chemicals