title: Characterization of ZAG protein expression in prostate cancer using a semi-automated microscope system.
abstract: OBJECTIVE
  Zinc-alpha-2-glycoprotein 1 (ZAG) is a 41-kD secreted protein that is known to stimulate lipid degradation in adipocytes. The aim of this study was to determine how ZAG protein expression is associated with prostate cancer (PCa).
  
  
  METHODS
  An immunohistochemistry analysis was performed on a 227 PCa tissue microarray cases. ZAG protein expression was assessed using a semi-automated cellular image analysis system.
  
  
  RESULTS
  ZAG expression was associated with tumor stage (pT2 > pT3 > metastasis cases, P < 0.001), and was inversely associated with Gleason score on pathology (P = 0.01). ZAG intensity was predictive of biochemical recurrence (P = 0.002). On multivariate analysis including pT2 patients, the predictive factors of biochemical recurrence were ZAG expression (P = 0.016), Gleason score (P = 0.011), and surgical margin status (P = 0.047).
  
  
  CONCLUSIONS
  This study characterized ZAG protein expression in PCa using a semi-automated system. ZAG expression level found to have an independent prognostic value for pT2 patients.
authors.affiliation: Department of Urology, Henri Mondor Hospital, Assistance Publique-Hopitaux de Paris, Paris, France.
authors.forename: Aurelien
authors.initials: A
authors.lastname: Descazeaud
authors.forename: Alexandre
authors.initials: A
authors.lastname: de la Taille
authors.forename: Yves
authors.initials: Y
authors.lastname: Allory
authors.forename: Hugo
authors.initials: H
authors.lastname: Faucon
authors.forename: Laurent
authors.initials: L
authors.lastname: Salomon
authors.forename: Tarek
authors.initials: T
authors.lastname: Bismar
authors.forename: Robert
authors.initials: R
authors.lastname: Kim
authors.forename: Matthias D
authors.initials: MD
authors.lastname: Hofer
authors.forename: Dominique
authors.initials: D
authors.lastname: Chopin
authors.forename: Claude C
authors.initials: CC
authors.lastname: Abbou
authors.forename: Mark A
authors.initials: MA
authors.lastname: Rubin
mesh_terms: D054392:Adipokines; D000368:Aged; D000369:Aged, 80 and over; D001331:Automation; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007150:Immunohistochemistry; D008297:Male; D008853:Microscopy / Q000379:methods*; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D011379:Prognosis; D011467:Prostate / Q000737:chemistry / Q000473:pathology; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D046888:Tissue Array Analysis; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D054392:Adipokines; D002352:Carrier Proteins; D006023:Glycoproteins; C488891:AZGP1 protein, human; D000090203:Zn-Alpha-2-Glycoprotein