title: Relative sensitivity factors of inorganic cations in frozen-hydrated standards in secondary ion MS analysis.
abstract: We describe the measurement, at 100 K, of the SIMS relative sensitivity factors (RSFs) of the main physiological cations Na+, K+, Mg2+, and Ca2+ in frozen-hydrated (F-H) ionic solutions. Freezing was performed by either plunge freezing or high-pressure freezing. We also report the measurement of the RSFs in flax fibers, which are a model for ions in the plant cell wall, and in F-H ionic samples, which are a model for ions in the vacuole. RSFs were determined under bombardment with neutral oxygen (FAB) for both the fibers and the F-H samples. We show that referencing to ice-characteristic secondary ions is of little value in determining RSFs and that referencing to K is preferable. The RSFs of Na relative to K and of Ca relative to Mg in F-H samples are similar to their respective values in fiber samples, whereas the RSFs of both Ca and Mg relative to K are lower in fibers than in F-H samples. Our data show that the physical factors important for the determination of the RSFs are not the same in F-H samples and in homogeneous matrixes. Our data show that it is possible to perform a SIMS relative quantification of the cations in frozen-hydrated samples with an accuracy on the order of 15%. Referencing to K permits the quantification of the ionic ratios, even when the absolute concentration of the referencing ion is unknown. This is essential for physiological studies of F-H biological samples.
authors.affiliation: Laboratoire Assemblages moléculaires: modélisation et imagerie SIMS (AMMIS), FRE CNRS 2829, Faculté des Sciences de l'Université de Rouen, 76821 Mont Saint Aignan Cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Dérue
authors.forename: D
authors.initials: D
authors.lastname: Gibouin
authors.forename: F
authors.initials: F
authors.lastname: Lefebvre
authors.forename: D
authors.initials: D
authors.lastname: Studer
authors.forename: M
authors.initials: M
authors.lastname: Thellier
authors.forename: C
authors.initials: C
authors.lastname: Ripoll