A carotenoid synthesis gene cluster from Algoriphagus sp. KK10202C with a novel fusion-type lycopene beta-cyclase gene.
A carotenoid synthesis gene cluster was isolated from a marine bacterium Algoriphagus sp. strain KK10202C that synthesized flexixanthin. Seven genes were transcribed in the same direction, among which five of them were involved in carotenoid synthesis. This cluster had a unique gene organization, with an isoprenoid gene, ispH (previously named lytB), being present among the carotenoid synthesis genes. The lycopene beta-cyclase encoded by the crtY ( cd ) gene appeared to be a fusion of bacterial heterodimeric lycopene cyclase CrtY(c) and CrtY(d). This was the first time that a fusion-type of lycopene beta-cyclase was reported in eubacteria. Heterologous expression of the Algoriphagus crtY ( cd ) gene in lycopene-accumulating Escherichia coli produced bicyclic beta-carotene. A biosynthesis pathway for monocyclic flexixanthin was proposed in Algoriphagus sp. strain KK10202C, though several of the carotenoid synthesis genes not linked with the cluster have not yet been cloned.
Tao
Luan
L
Biological and Chemical Sciences and Engineering, Central Research and Development, E. I. DuPont. de Nemours Inc., Experimental Station, E328/B48, Wilmington, DE 19880-0328, USA.
Yao
Henry
H
Kasai
Hiroaki
H
Misawa
Norihiko
N
Cheng
Qiong
Q
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002338:Carotenoids / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D041964:Cytophagaceae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism*; D000077276:Lycopene; D008969:Molecular Sequence Data; D005810:Multigene Family; D017386:Sequence Homology, Amino Acid; D019207:beta Carotene / Q000378:metabolism*
D001426:Bacterial Proteins; D019207:beta Carotene; D002338:Carotenoids; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase; D000077276:Lycopene