title: High-throughput genotyping with infrared fluorescence allele specific hybridization (iFLASH): a simple, reliable and low-cost alternative.
abstract: OBJECTIVE
  To develop and validate a novel genotyping approach, named infrared Fluorescence Allele Specific Hybridization (iFLASH), which combines the principles of allele specific oligonucleotide (ASO) hybridization with the advanced possibilities of infrared imaging.
  
  
  METHODS
  As an example, we genotyped the 55L > M and the 192Q > R common genetic variants of the paraoxonase-1 gene in 92 DNA samples using the iFLASH technique, and validated the outcomes with the restriction fragment length polymorphism (RFLP) and TAQman genotyping assays.
  
  
  RESULTS
  There was a 100 percent agreement in genotype outcome among the three methods.
  
  
  CONCLUSIONS
  Although we found complete unity in genotype outcome, the iFLASH assay has essential advantages over the RFLP and TAQman genotyping assays. First, the iFLASH technique is capable of handling up to 1536 samples per assay, which makes it a suitable technique for high-throughput genotyping. Secondly, because the costs per assay are lower, high-throughput genotyping with iFLASH is affordable.
authors.affiliation: Research Laboratory of the Department of Clinical Chemistry, University Medical Center Utrecht, Utrecht, The Netherlands. T.M.vanHimbergen@azu.nl
authors.forename: Thomas M
authors.initials: TM
authors.lastname: van Himbergen
authors.forename: Hieronymus A M
authors.initials: HA
authors.lastname: Voorbij
authors.forename: Arjan D
authors.initials: AD
authors.lastname: Barendrecht
authors.forename: Bas B
authors.initials: BB
authors.lastname: van Rijn
authors.forename: Roberta
authors.initials: R
authors.lastname: Brambilla
authors.forename: Lambertus J H
authors.initials: LJ
authors.lastname: van Tits
authors.forename: Mark
authors.initials: M
authors.lastname: Roest
mesh_terms: D000483:Alleles; D043303:Aryldialkylphosphatase / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000191:economics* / Q000379:methods*; D007259:Infrared Rays; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted
chemical_list: D043303:Aryldialkylphosphatase; C468406:PON1 protein, human