Development of the rapid and simple elisa (whole blood-ELISA) using coconut water-tween as a wash solution for whole blood sample from Schistosoma japonicum-infected rabbit and human.
PBS-Tween as a wash solution, prepared with distilled water, is used in ELISA. In areas where schistosomiasis is endemic, however, distilled water is hard to come by. We have modified a WHOLE BLOOD-ELISA test to use coconut water-Tween as a wash solution, because coconut water is easy to come by and cheap in the tropics. We applied the test to whole blood samples from rabbits and humans infected with Schistosoma japonicum. This modified WHOLE BLOOD-ELISA was confirmed to be a rapid, simple, and cost-effective method.
Hirose
Yoshinori
Y
Department of Tropical Medicine and Parasitology, Dokkyo University School of Medicine, Mibu, Shimotsuga, Tochigi, Japan.
Lipayon
Ignacio L
IL
Kirinoki
Masashi
M
Chigusa
Yuichi
Y
Matsuda
Hajime
H
D000818:Animals; D003059:Cocos; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008403:Mass Screening / Q000379:methods; D011817:Rabbits; D012549:Schistosoma japonicum / Q000201:enzymology / Q000302:isolation & purification*; D012552:Schistosomiasis / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology; D012698:Serologic Tests / Q000379:methods; D012996:Solutions
D012996:Solutions