The effect of cyanoacrylic glue on rat testis, urethra and spongiosal tissue: an experimental study.
OBJECTIVE
We examined the effect of Glubran2 on the rat urethra and spongiosal tissue as well as the histopathological effect. We also investigated its clinical use.


METHODS
Eight control and 12 study group male Wistar rats were used in our study. The corpus spongiosum tissues, including the urethra and the tunica albuginea of the testis, were cut 0.5 and 0.2 cm long, respectively. Incision areas in the control group were sutured. In the study group Glubran2 was dripped on the area. Three weeks later all rats were sacrificed. The penile and testicular tissues were histopathologically investigated. One-way ANOVA and the t test were applied for statistical analysis.


RESULTS
When the study group was compared with the control group, the difference in urethral healing (p = 0.001), urethral lumen irregularity (p = 0.036), spongiosal tissue healing (p = 0.001), inflammation (p = 0.000), hyperemia-bleeding (p = 0.036) and total healing (p = 0.001) were statistically significant. When the study and control groups were compared, the difference in inflammation (p = 0.000), necrotic areas (p = 0.002), tunica albuginea irregularity (p = 0.005), Sertoli's and Leydig cell destruction (p = 0.005), and testicular spermatogenesis (p = 0.005) were statistically significant.


CONCLUSIONS
While perfect healing in the urethral and spongiosal tissues supports the clinical application, damage to the seminiferous tubules, decreased spermatogenesis at the operated site, tunica albuginea irregularity in a fourth of the testes and calcification in 1 testis were observed.
Ayyildiz
Ali
A
Urology Clinic, Ministry of Health Ankara Training and Education Hospital, Ankara, Turkey. urology52@yahoo.com
Nuhoğlu
Bariş
B
Cebeci
Ozden
O
Caydere
Muzaffer
M
Ustün
Hüseyin
H
Germiyanoğlu
Cankon
C
D000818:Animals; D003487:Cyanoacrylates / Q000494:pharmacology*; D008297:Male; D010413:Penis / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D014521:Urethra / Q000187:drug effects* / Q000473:pathology
D003487:Cyanoacrylates; C421461:glubran 2