title: Sequence analysis of the non-structural 3A and 3C protein-coding regions of foot-and-mouth disease virus serotype Asia1 field isolates from an endemic country.
abstract: A total of 18 foot-and-mouth disease virus (FMDV) serotype Asia1 field isolates belonging to two different lineages (including the divergent group) as delineated earlier in VP1-based phylogeny were sequenced in the non-structural 3A and 3C protein-coding regions. The phylogenetic trees representing the regions coding for the non-structural proteins were very similar to that of the structural VP1 protein-coding region. Phylogenetic comparison at 3C region revealed clustering of Asia1 viruses with the isolates of serotypes O, A and C in the previously identified clade. Comparison of amino acid sequences identified lineage-specific signature residues in both the non-structural proteins. Overall analysis of the amino acid substitutions revealed that the 3A coding region was more prone to amino acid alterations than 3C region.
authors.affiliation: Project Directorate on Foot-and-Mouth Disease, Indian Veterinary Research Institute Campus, Mukteswar-Kumaon, Nainital 263 138, Uttaranchal, India.
authors.forename: S
authors.initials: S
authors.lastname: Biswas
authors.forename: A
authors.initials: A
authors.lastname: Sanyal
authors.forename: D
authors.initials: D
authors.lastname: Hemadri
authors.forename: C
authors.initials: C
authors.lastname: Tosh
authors.forename: J K
authors.initials: JK
authors.lastname: Mohapatra
authors.forename: R
authors.initials: R
authors.lastname: Manoj Kumar
authors.forename: S K
authors.initials: SK
authors.lastname: Bandyopadhyay
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D007194:India; D010802:Phylogeny; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D017361:Viral Nonstructural Proteins