Human subcutaneous adipose cells support complete differentiation but not self-renewal of hematopoietic progenitors.
Adipose tissue is now considered as an endocrine organ implicated in energy regulation, inflammation and immune response, and as a source of multipotent cells with a broad range of differentiation capacities. Some of these cells are of a mesenchymal type which can -- like their bone marrow (BM) counterpart -- support hematopoiesis, since in a previous study we were able to reconstitute lethally irradiated mice by cells isolated from adipose tissue. In the present study, we established that cells derived from the stroma-vascular fraction of human subcutaneous fat pads support the complete differentiation of hematopoietic progenitors into myeloid and B lymphoid cells. However, these cells are unable to maintain the survival and self-renewal of hematopoietic stem cells. These features, similar to those of BM adipocytes, are the opposite of those of other cell types derived from mesenchymal progenitors such as BM myofibroblasts or osteoblasts. Because it is abundant and accessible, adipose tissue could be a convenient source of cells for the short-term reconstitution of hematopoiesis in man.
Corre
Jill
J
UMR-CNRS 5018, Institut Louis Bugnard, CHU Toulouse Rangueil, Toulouse, France.
Barreau
Corinne
C
Cousin
Beatrice
B
Chavoin
Jean-Pierre
JP
Caton
David
D
Fournial
Gerard
G
Penicaud
Luc
L
Casteilla
Louis
L
Laharrague
Patrick
P
D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry; D016207:Cytokines / Q000032:analysis; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008875:Middle Aged; D022423:Myeloid Cells / Q000145:classification / Q000166:cytology; D017154:Stromal Cells / Q000166:cytology
D018952:Antigens, CD34; D003470:Culture Media; D016207:Cytokines