[Quantification of caffeine from energy drinks with mass spectrometry method (LC/MS)].
A high performance liquid chromatography coupled with mass spectrometry method (LC/MS) for quantification of caffeine from energy drinks was elaborated. It was utilised a Zorbax SB-C18, 100 mm x 3.0 mm i.d., 3.5 microm column with a mobile phase containing methanol/ solution 0.2% formic acid in water. Detection and quantification of caffeine was based on monitoring the protonated molecular ion abundance (m/z 194.9). The quantification was made using the external standard method. The calibration curve were made on range 0.26-26 microg/ml for caffeine with a correlation coefficient greater than 0.995. There were analysed 12 energy drinks with a caffeine content between 13 and 34 mg/100 ml.
Filip
Lorena
L
Universitatea de Medicină şi Farmacie Iuliu Haţieganu Cluj-Napoca, Facultatea de Farmacie, Disciplina de Chimie Sanitară-Igienă.
Vlase
L
L
Mîndruţau
Ioana
I
Miere
Doina
D
D001628:Beverages / Q000032:analysis*; D002110:Caffeine / Q000032:analysis*; D000697:Central Nervous System Stimulants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D000697:Central Nervous System Stimulants; D002110:Caffeine