Expression profiling of cyclin B1 and D1 in cervical carcinoma.
OBJECTIVE
Cyclins are a family of regulatory proteins that play a key role in controlling the cell cycle. Abnormalities of cell cycle regulators, including cyclins and cyclin dependent kinases, have been reported in various malignant tumors. This study was undertaken to quantitatively detect cyclin B1 and D1 in cervical cancer.


METHODS
A quantitative real-time reverse transcription polymerase chain reaction and Western blot assay were used to analyze the expression of cyclin B1/D1 mRNA and proteins, respectively, in fresh invasive cervical cancer (n = 41) and normal cervical tissues (n = 10).


RESULTS
There was significantly greater cyclin B1 expression in invasive cervical cancer than in normal cervical tissue (P = 0.019). However, cyclin D1 expression was not significantly different. A Western blot assay yielded similar results.


CONCLUSIONS
Our results were consistent with the concept that up-regulation of cyclin B1 expression occurred in cervical cancer and an aberrant expression of cyclin B1 might play an important role in cervical carcinogenesis.
Zhao
M
M
Department of Obstetrics and Gynecology, Women's life and Science Institute, Yonsei University College of Medicine, Seoul, Korea.
Kim
Y T
YT
Yoon
B S
BS
Kim
S W
SW
Kang
M H
MH
Kim
S H
SH
Kim
J H
JH
Kim
J W
JW
Park
Y W
YW
D015153:Blotting, Western; D002584:Cervix Uteri / Q000502:physiology; D019926:Cyclin B / Q000235:genetics* / Q000378:metabolism; D056744:Cyclin B1; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D012334:RNA, Neoplasm / Q000235:genetics; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
C534699:CCNB1 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D018076:DNA, Complementary; D012334:RNA, Neoplasm; D019938:Cyclin D1