Diagnostic impact of the genetic variability of the hepatitis B virus surface antigen gene.
The genetic variability of hepatitis B virus (HBV) represents a challenge for the sensitivity of immunologic and molecular based assays. Genotyping studies show that the genetic diversity of HBV is very high even in industrialized countries. The analytical sensitivity of HBsAg and anti-HBs assays may be dependent on HBV genotype or subtype and could possibly lead to false negative results in samples with low-level HBsAg. It is possible that the recognition of genotypes E and F may be impaired. Immunoassays based on polyclonal capture antibody show the highest sensitivity for the recognition of recombinant mutants or serum samples harboring mutant forms of HBsAg. However, they do not guarantee full sensitivity, especially for the detection of the G145R mutation and amino acid insertions or substitutions in positions 120-123. Detection of HBsAg needs to be improved by the introduction of new HBsAg assays able to recognize so far described S gene mutants and with a lower detection threshold than current immunoassays in order to detect smallest amounts of HBsAg in low level carriers. There is also a need for more complete epidemiological data on the prevalence of HBsAg mutants especially for G145R and assays for the (differential) screening of mutants need to be developed and evaluated.
Weber
Bernard
B
Laboratoires Réunis Junglinster, Luxemburg und Institut für Medizinische Virologie, Universitätskliniken Frankfurt, Frankfurt, Germany. Web@labo.lu
D005188:False Negative Reactions; D005798:Genes, Bacterial; D014644:Genetic Variation; D006508:Hepatitis Antibodies; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D014773:Virology / Q000379:methods*
D006508:Hepatitis Antibodies; D006514:Hepatitis B Surface Antigens