title: Proteomic analysis of anaplastic lymphoma cell lines: identification of potential tumour markers.
abstract: Anaplastic large-cell lymphomas (ALCL) are high grade lymphomas of T or null phenotype often associated with the t(2;5) translocation leading to the expression of a chimeric protein consisting of the N-terminal portion of nucleophosmin (NPM) and the intracellular domain of the anaplastic lymphoma kinase (ALK). Although ALCL are recognized as distinct clinical, biological and cytogenetic entities, heterogeneities persist in this group of tumours, which exhibit a broad spectrum of morphological features. Particularly, the common type tumour consisting in large cells contrast with the small cell variant that is sometimes associated with a leukemic phase. The ALK-negative ALCL is often associated with a poor prognosis. Here, we investigated the proteome of these subtypes of tumours using patient-derived cell lines. We compared the proteome of the cytosolic fraction of NPM-ALK-positive versus NPM-ALK-negative cells on one hand, and the proteome of common cell type versus small cell variant on the other hand. The identification of a set of proteins differentially expressed in the subtypes of ALCL points to new diagnosis/prognosis markers. This study also provides interesting information on the molecular mechanisms responsible for the different subtypes of ALCL.
authors.affiliation: INSERM U563, Centre de Physiopathologie de Toulouse Purpan, Département d'Oncogenèse et Signalisation dans les Cellules Hématopoïétiques, CHU Purpan, 31059 Toulouse, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cussac
authors.forename: Carole
authors.initials: C
authors.lastname: Pichereaux
authors.forename: Audrey
authors.initials: A
authors.lastname: Colomba
authors.forename: Florence
authors.initials: F
authors.lastname: Capilla
authors.forename: Frédéric
authors.initials: F
authors.lastname: Pont
authors.forename: Frédérique
authors.initials: F
authors.lastname: Gaits-Iacovoni
authors.forename: Laurence
authors.initials: L
authors.lastname: Lamant
authors.forename: Estelle
authors.initials: E
authors.lastname: Espinos
authors.forename: Odile
authors.initials: O
authors.lastname: Burlet-Schiltz
authors.forename: Bernard
authors.initials: B
authors.lastname: Monsarrat
authors.forename: Georges
authors.initials: G
authors.lastname: Delsol
authors.forename: Bernard
authors.initials: B
authors.lastname: Payrastre
mesh_terms: D000077548:Anaplastic Lymphoma Kinase; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D003600:Cytosol / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007150:Immunohistochemistry; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000096:biosynthesis; D000090243:Nucleophosmin; D010641:Phenotype; D011379:Prognosis; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis; D020543:Proteome / Q000096:biosynthesis*; D020794:Receptor Protein-Tyrosine Kinases; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D020543:Proteome; D000090243:Nucleophosmin; C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases