Cloning and production of a novel bacteriocin, lactococcin K, from Lactococcus lactis subsp. lactis MY23.
A gene encoding the antimicrobial peptide, lactococcin K, was isolated from Lactococcus lactis subsp. lactis MY23 then cloned and expressed in Escherichia coli. Because the expressed lactococcin K was formed as an inclusion body in recombinant E. coli, a fusion protein containing lactococcin K and maltose-binding protein (MBP) was produced in a soluble form. For high-level production of lactococcin K, we performed a pH-stat fed-batch culture to produce 43,000 AU lactococcin K ml(-1) in 12 h.
Kim
Yun Seog
YS
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Guseong-dong, 373-1, Yusong-gu, Daejeon, 305-701, Korea.
Kim
Min Ju
MJ
Kim
Pil
P
Kim
Jung Hoe
JH
D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000737:chemistry* / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods*; D014162:Transfection / Q000379:methods
D001430:Bacteriocins; C069325:lactococcin A