title: Picoliter-volume aqueous droplets in oil: electrochemical detection and yeast cell electroporation.
abstract: An electrochemical detection method was introduced for aqueous droplet analysis in oil phase of microfluidic devices. This method is based on the electrochemical signal difference between aqueous and oil. Applying a low alternating current (AC) voltage to a couple of Au microelectrodes, this method can offer size information and ion concentration range from 0.02 mmol/L to 1 mol/L of tens of picoliter to nanoliter aqueous droplets. Alternatively, applying a relative high AC voltage (18 Vpp) at a frequency of 1 kHz leads to electroporation of yeast cells encapsulated into picoliter droplets. We believe that this simple technique is useful for a number of aqueous droplet-based chemical and biological analyses as well as cell electroporation.
authors.affiliation: Center for Microfluidic and Nanotechnology, School of Physics, Peking University, Beijing, China.
authors.forename: Chunxiong
authors.initials: C
authors.lastname: Luo
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Manhui
authors.initials: M
authors.lastname: Sun
authors.forename: Qi
authors.initials: Q
authors.lastname: Ouyang
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Hang
authors.initials: H
authors.lastname: Ji
mesh_terms: D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D018274:Electroporation; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D009821:Oils; D012441:Saccharomyces cerevisiae; D014867:Water
chemical_list: D009821:Oils; D014867:Water