title: [Screening for Y chromosomal microdeletions in AZF region with modified multiplex PCR].
abstract: OBJECTIVE
  Screening for Y chromosomal microdeletions in azoospermia factor (AZF) region with modified multiplex PCR.
  
  
  METHODS
  160 cases with spermatogenetic failure were recruited in the experimental group, while 90 cases of donors in controls. According to the laboratory guidelines supported by European Academy of Andrology (EAA) and European Molecular Genetics Quality Network (EMQN), Y chromosomal microdeletions in AZFa, b, c regions were screened with multiplex PCR. The primers of sequence targeted sites (STSs) and conditions of PCR were modified.
  
  
  RESULTS
  Using modified multiplex PCR, 14 (8.75%) cases with Y chromosomal microdeletions were found in the experimental group, while no case in controls. There were 12 cases in AZFc, 1 case in AZFa + b + c, 1 case in AZFb + c. According to statistics, the difference between two groups was significant (P <0.001). Reaction products could be clearly separated with agarose gel and finished in 1 h.
  
  
  CONCLUSIONS
  Modified multiplex PCR protocols supported by EAA and EMQN proved to be very accurate, sensitive and quick, which could be put into screening practice for Y chromosomal microdeletions in AZF region.
authors.affiliation: Shanghai Institute of Andrology, Shanghai Human Sperm Bank, Renji Hospital Affiliated to Shanghai Second Medical University, China.
authors.forename: Xiao-bin
authors.initials: XB
authors.lastname: Zhu
authors.forename: An-liang
authors.initials: AL
authors.lastname: Guo
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Cao
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Xu-xu
authors.initials: XX
authors.lastname: Sun
authors.forename: Jian-er
authors.initials: JE
authors.lastname: Yao
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yi-xin
authors.initials: YX
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
mesh_terms: D000328:Adult; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D056426:Genetic Loci; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D029589:Seminal Plasma Proteins / Q000235:genetics
chemical_list: D029589:Seminal Plasma Proteins