title: Microfabricated discontinuous-edge surface topographies influence osteoblast adhesion, migration, cytoskeletal organization, and proliferation and enhance matrix and mineral deposition in vitro.
abstract: The fabrication of surfaces that stimulate increased adhesion, migration, and differentiated function of osteoblasts has been viewed as being desirable for many orthopedic applications. Previous studies have shown that microfabricated pits and grooves alter adhesion, spreading, matrix secretion, and production of mineral by rat calvarial osteoblasts (RCOs). The mechanisms underlying these effects are unknown, although microenvironment and cell alignment are considered to play a role. The aim of this work was to investigate the behavior of RCOs on microfabricated discontinuous-edge surfaces (DESs), which could provide an alternative means to control both the microenvironment and cellular alignment. Two types of discontinuous-type structures were employed, gap-cornered boxes and micron scale pillars. DES gap-cornered boxes and the pillars influenced the arrangement of F-actin, microtubules, and vinculin. Osteoblasts were guided in their direction of migration on both types of substrata. Both box DESs and pillars altered the staining intensity and localization pattern of phosphotyrosine and src-activated FAK localization. Cell multilayering, matrix deposition, and mineralization were enhanced on both discontinuous topographies when compared with smooth controls. This study shows that DESs alter adhesion, migration, and proliferative responses from osteoblasts at early time points (<1 week) and promote multilayering, matrix deposition, and mineral deposition at later times (2-6 weeks). Such topographical patterns could potentially be employed as effective surface features on bone-contacting implants or in membrane-based periodontal applications.
authors.affiliation: Department of Oral, Biological, and Medical Sciences, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
authors.forename: D W
authors.initials: DW
authors.lastname: Hamilton
authors.forename: K S
authors.initials: KS
authors.lastname: Wong
authors.forename: D M
authors.initials: DM
authors.lastname: Brunette
mesh_terms: D000199:Actins / Q000378:metabolism / Q000648:ultrastructure; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001857:Bone Matrix / Q000378:metabolism* / Q000648:ultrastructure; D001861:Bone Regeneration / Q000502:physiology*; D002113:Calcification, Physiologic / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D004853:Epoxy Resins / Q000627:therapeutic use; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D018715:Microscopy, Video; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D010006:Osteoblasts / Q000378:metabolism* / Q000648:ultrastructure; D010012:Osteogenesis / Q000502:physiology*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D051381:Rats; D012886:Skull / Q000378:metabolism / Q000648:ultrastructure; D016596:Vinculin / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000199:Actins; D004853:Epoxy Resins; D016596:Vinculin; D019000:Phosphotyrosine; D051416:Focal Adhesion Protein-Tyrosine Kinases; D000469:Alkaline Phosphatase