PIN proteins perform a rate-limiting function in cellular auxin efflux.
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
Petrásek
Jan
J
Institute of Experimental Botany, the Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic.
Mravec
Jozef
J
Bouchard
Rodolphe
R
Blakeslee
Joshua J
JJ
Abas
Melinda
M
Seifertová
Daniela
D
Wisniewska
Justyna
J
Tadele
Zerihun
Z
Kubes
Martin
M
Covanová
Milada
M
Dhonukshe
Pankaj
P
Skupa
Petr
P
Benková
Eva
E
Perry
Lucie
L
Krecek
Pavel
P
Lee
Ok Ran
OR
Fink
Gerald R
GR
Geisler
Markus
M
Murphy
Angus S
AS
Luschnig
Christian
C
Zazímalová
Eva
E
Friml
Jirí
J
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D018522:Gravitropism; D006367:HeLa Cells; D006801:Humans; D007210:Indoleacetic Acids / Q000378:metabolism*; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009280:Naphthaleneacetic Acids / Q000378:metabolism; D010797:Phthalimides / Q000494:pharmacology; D018517:Plant Roots / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014026:Nicotiana; D014162:Transfection; D014170:Transformation, Genetic
D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D009280:Naphthaleneacetic Acids; D010797:Phthalimides; C114215:ATPGP1 protein, Arabidopsis; C116363:PIN1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis; C451260:PIN4 protein, Arabidopsis; C479554:PIN7 protein, Arabidopsis; C002687:alpha-naphthylphthalamic acid; C034182:1-naphthaleneacetic acid