In vitro panning of a targeting peptide to hepatocarcinoma from a phage display peptide library.
Phage display technology has been used as a powerful tool in the discovery of ligands specific to receptor(s) on the surface of a cancer cell and could also impact clinical issues including functional diagnosis and cell-specific drug delivery. After three rounds of in vitro panning and two rounds of reverse absorption, a group of phages capable of addressing BEL-7402 enormously were obtained for further analysis. Through a cell-based ELISA, immunofluorescence, FACS, and in vivo binding study, WP05 (sequence TACHQHVRMVRP) was demonstrated to be the most effective peptide in targeting four kinds of liver cancer cell lines (BEL-7402, BEL-7404, SMMC-7721, and HepG2), but not the normal liver cell line HL-7702. In conclusion, the peptide WP05 which was screened by in vitro phage display technology was proved to be a targeting peptide to several common hepatocellular carcinoma cell lines.
Du
Bing
B
School of Life Sciences, East China Normal University, Shanghai, PR China.
Qian
Min
M
Zhou
Zhongliang
Z
Wang
Peng
P
Wang
Lei
L
Zhang
Xiaoping
X
Wu
Miao
M
Zhang
Ping
P
Mei
Bing
B
D000595:Amino Acid Sequence; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007267:Injections; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding
D019151:Peptide Library; D010455:Peptides