The effect of medicinal plants used in Chinese folk medicine on RANTES secretion by virus-infected human epithelial cells.
The accumulation of inflammatory cells in the infective sites has been reported to play a crucial role in the progression of chronic inflammation and multiple sclerosis after viral infection. In the present study, nine ethanol extracts of Forsythia suspensa Vahl. (Oleaceae), Lonicera japonica Thunb. (Caprifoliaceae), Isatis indigotica Fort. (Cruciferae), Strobilanthes cusia (Ness.) O. Kuntze (Acanthaceae), Astragalus membranaceus (Fisch.) Bge. (Leguminosae), Hedysarum polybotrys Hand.-Mazz. (Leguminosae), Andrographis paniculata (Burm. f.) Ness. (Acanthaceae), Glycyrrhiza uralensis Fischer. (Leguminosae) and Ligusticum wallichii Franch. (Umbelliferae), medicinal plants traditionally used in China for treating conditions likely to be associated with inflammation and viral infection, were screened for their effect on RANTES secretion by influenza A virus (H1N1)-infected human bronchial epithelial cells (A549). With exception of Lonicera japonica, Isatis indigotica, Astragalus membranaceus and Hedysarum polybotrys, all plants tested at concentration of 200 microg/ml possessed more than 50% suppressing effect on RANTES secretion by H1N1-infected A549 bronchial epithelial cells. Among the plants tested, Andrographis paniculata showed the most promising property to inhibit RANTES secretion with an IC(50) of 1.2 +/- 0.4 microg/ml while the next two were Glycyrrhiza uralensis and Forsythia suspensa (IC(50) ranging from 35 to 48 microg/ml).
Ko
Han-Chieh
HC
Division of Basic Chinese Medical Research, National Research Institute of Chinese Medicine, Shipai, Taipei, Taiwan, ROC.
Wei
Bai-Luh
BL
Chiou
Wen-Fei
WF
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology / Q000821:virology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000472:pathogenicity*; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal
D018946:Chemokine CCL5; D004365:Drugs, Chinese Herbal