Expression of discoidin domain receptor 1 during mouse brain development follows the progress of myelination.
Discoidin domain receptor 1 is a tyrosine kinase receptor expressed in a variety of tissues including the brain. This study describes mRNA and protein expression of discoidin domain receptor 1 in mouse brain during development and provides new insights into its role during gliogenesis and neurogenesis. We performed in situ hybridization for discoidin domain receptor 1 in mouse brains at embryonic day 18, postnatal days 5, 9, 15, 21 and adulthood and observed a diffuse pattern in the proliferative areas during embryogenesis. From postnatal day 5 onwards, a defined cellular expression pattern of discoidin domain receptor 1 was observed, mainly located in white matter tracts and following a spatio-temporal pattern that overlapped the progress of myelination. Next, we performed double-labeling reactions (in situ hybridization followed by immunohistochemistry) that confirmed that discoidin domain receptor 1 was expressed by mature oligodendrocytes. We observed that cells positive for discoidin domain receptor 1 also expressed carnosine and anti-adenomatous polyposis coli, two mature oligodendrocyte markers. Based on the localization of discoidin domain receptor 1 specifically in the white matter fiber tracts during postnatal development, we suggest that discoidin domain receptor 1 participates in the development and maintenance of the myelin sheath.
Franco-Pons
N
N
Unitat de Psiquiatria i Psicologia Mèdica, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, and Departament de Formació i Investigació, Hospital Psiquiàtric Universitari Institut Pere Mata, Reus, Spain.
Virgos
C
C
Vogel
W F
WF
Ureña
J M
JM
Soriano
E
E
del Rio
J A
JA
Vilella
E
E
D025601:Adenomatous Polyposis Coli Protein / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D002336:Carnosine / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D000070818:Discoidin Domain Receptors; D047108:Embryonic Development / Q000502:physiology; D051379:Mice; D009186:Myelin Sheath / Q000378:metabolism* / Q000648:ultrastructure; D009413:Nerve Fibers, Myelinated / Q000378:metabolism* / Q000648:ultrastructure; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011975:Receptors, Mitogen / Q000235:genetics / Q000378:metabolism*
D025601:Adenomatous Polyposis Coli Protein; D015415:Biomarkers; D012333:RNA, Messenger; D011975:Receptors, Mitogen; D002336:Carnosine; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases