[Research of RhD protein in Rh blood group Del phenotype].
OBJECTIVE
To study the expression of RhD protein in Rh blood group Del phenotype.


METHODS
Peripheral blood samples were collected from 3 voluntary blood donors with different Rh phenotypes: CCD(el)D(el)ee, CcD(el)dee, and CcD(el)D(el)ee, and 4 controls: 2 RhD positive with the phenotypes of CCDDee and CcDDEe, and 2 RhD negative with the phenotypes: Ccddee and ccddee. Reverse transcriptase PCR (RT-PCR) and cDNA sequencing were used to detect the RhD mRNA and the expression of Del protein.


RESULTS
The Del individuals all showed complicated RhD mRNA isolations, including 6 transcripts: isoforms with exons 7-9 spliced, exons 7 and 9 spliced, exons 8 and 9 spliced, exon 9 spliced and 2 long transcripts with exons 8 and 9 spliced or exon 9 spliced, but containing an additional segment of sequence from RHD intron 7 (917-1086, GenBank AB035194), those transcripts all being out of exon 9 and the Del9 transcript being the most similar to the normal RhD mRNA.


CONCLUSIONS
Normal RhD mRNA does not exist in the Del blood group that does not code normal RhD protein.
Xiong
Wen
W
Shenzhen Institute of Transfution Medicine, Shenzhen Blood Center, Shenzhen 518035, China.
Shao
Chao-peng
CP
Li
Xue-mei
XM
Zhou
Yi-yan
YY
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D001782:Blood Donors; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012204:Rh-Hr Blood-Group System / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D020033:Protein Isoforms; D012333:RNA, Messenger; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen