Serotonergic and nonserotonergic neurons in the dorsal raphe nucleus send collateralized projections to both the vestibular nuclei and the central amygdaloid nucleus.
Using a combination of double retrograde tracing and serotonin immunofluorescence staining, we examined whether individual serotonergic and nonserotonergic neurons in the dorsal raphe nucleus are sources of collateralized axonal projections to vestibular nuclei and the central amygdaloid nucleus in the rat. Following unilateral injections of Diamidino Yellow into the vestibular nuclei and Fast Blue into the central amygdaloid nucleus, it was observed that approximately one-fourth of the dorsal raphe nucleus neurons projecting to the vestibular nuclei send axon collaterals to the central amygdaloid nucleus. Immunofluorescence staining for serotonin revealed that more than half of the dorsal raphe nucleus neurons from which these collateralized projections arise contain serotonin-like immunoreactivity. These findings indicate that a subpopulation of serotonergic and nonserotonergic dorsal raphe nucleus cells may act to co-modulate processing in the vestibular nuclei and the central amygdaloid nucleus, regions implicated in the generation of emotional and affective responses to real and perceived motion.
Halberstadt
A L
AL
Department of Neurobiology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Balaban
C D
CD
D000578:Amidines; D000679:Amygdala / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001008:Anxiety Disorders / Q000209:etiology / Q000503:physiopathology; D001370:Axonal Transport / Q000502:physiology; D004525:Efferent Pathways / Q000166:cytology* / Q000378:metabolism; D004644:Emotions / Q000502:physiology; D008297:Male; D008636:Mesencephalon / Q000166:cytology* / Q000378:metabolism; D009039:Motion Perception / Q000502:physiology; D004856:Postural Balance / Q000502:physiology; D017729:Presynaptic Terminals / Q000502:physiology / Q000648:ultrastructure; D011903:Raphe Nuclei / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D012701:Serotonin / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology; D015837:Vestibular Diseases / Q000150:complications / Q000503:physiopathology; D014726:Vestibular Nuclei / Q000166:cytology* / Q000378:metabolism
D000578:Amidines; C031455:diamidino compound 253-50; D012701:Serotonin; C037945:diamidino yellow