Microcystin analysis in single filaments of Planktothrix spp. in laboratory cultures and environmental blooms.
Single filaments of Planktothrix spp. were isolated from laboratory cultures of P. agardhii (NIES 595) and P. rubescens (SL 03) and from four freshwater lakes in England and Turkey. Filament lengths were measured and microcystins were extracted by freeze-thawing and boiling. Microcystin analysis of the isolated single filaments was performed by ELISA using antibodies raised against microcystin-LR with a minimum detection limit (MDL) of 11 pg filament(-1). In some cases a high percentage of the filaments from the environmental samples and laboratory cultures were below the MDL of the assay. Based on the filaments with detectable microcystin contents, P. agardhii from Bassenthwaite Lake (England) had the lowest mean microcystin concentration (0.7 fg microm(-3)), and the highest microcystin concentration (2.9 fg microm(-3)) was measured in P. rubescens from Iznik Lake (Turkey). We investigated the relationship for filaments with microcystin contents above MDL and their biovolume. Relationships varied widely although P. agardhii from Bassenthwaite showed a better (positive) relationship between filament biovolume and microcystin content than P. rubescens from environmental samples. Under culture conditions, P. rubescens showed a good relationship between filament biovolume and toxin content.
Akcaalan
Reyhan
R
Istanbul University, Fisheries Faculty, Ordu Cad. No:200 34470 Laleli, Istanbul, Turkey. akcaalan@istanbul.edu.tr
Young
Fiona M
FM
Metcalf
James S
JS
Morrison
Louise F
LF
Albay
Meric
M
Codd
Geoffrey A
GA
D000458:Cyanobacteria / Q000737:chemistry* / Q000254:growth & development; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D014871:Water Microbiology
D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR