title: Adhesion of Borrelia garinii to neuronal cells is mediated by the interaction of OspA with proteoglycans.
abstract: To study pathogenic mechanisms of Lyme meningoradiculitis, dorsal root ganglia (DRG) cells and two neuronal cell lines (B50, SH-SY5Y) were incubated with Borrelia garinii, the Borrelia species most frequently isolated from CSF of Lyme neuroborreliosis patients in Europe. We demonstrated that (I) OspA-positive B. garinii adhere to neuronal cells, (II) Borrelia adhesion can be blocked by a monoclonal antibody against OspA, (III) preincubation with proteoglycans interferes with the adhesion process and (IV) rOspA directly binds to the proteoglycans. This indicates that both OspA and the cell bound proteoglycans are involved in the attachment of B. garinii to neuronal cells.
authors.affiliation: Department of Neurology, Klinikum Grosshadern, Ludwig-Maximilians University, Marchioninistr. 15, D-81377 Munich, Germany.
authors.forename: Tobias A
authors.initials: TA
authors.lastname: Rupprecht
authors.forename: Uwe
authors.initials: U
authors.lastname: Koedel
authors.forename: Christiane
authors.initials: C
authors.lastname: Heimerl
authors.forename: Volker
authors.initials: V
authors.lastname: Fingerle
authors.forename: Robert
authors.initials: R
authors.lastname: Paul
authors.forename: Bettina
authors.initials: B
authors.lastname: Wilske
authors.forename: Hans-Walter
authors.initials: HW
authors.lastname: Pfister
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001428:Bacterial Vaccines; D015748:Borrelia burgdorferi Group / Q000302:isolation & purification / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D005727:Ganglia, Spinal / Q000378:metabolism; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D020852:Lyme Neuroborreliosis / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats
chemical_list: D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D015815:Cell Adhesion Molecules; D008074:Lipoproteins; C072045:OspA protein; D011509:Proteoglycans