title: h-Caldesmon, a useful positive marker in the diagnosis of pleural malignant mesothelioma, epithelioid type.
abstract: Although a large number of immunohistochemical markers that can facilitate the differential diagnosis between epithelioid pleural mesothelioma and lung adenocarcinoma involving the pleura have proven to be valuable, no single antibody has demonstrated absolute sensitivity and/or specificity in making this distinction. Using immunohistochemical analysis with h-caldesmon, a specific marker for smooth muscle tumors, we examined 70 cases of epithelial mesotheliomas and 70 cases of lung adenocarcinomas. In addition, immunohistochemistry for muscle markers, such as desmin, alpha-smooth-muscle actin, muscle-specific actin, myoglobin, myogenin, myosin, and MyoD-1, was performed on all mesothelioma cases. Reactivity for h-caldesmon was obtained in 68 (97%) of the 70 epithelial mesotheliomas, but in none of the adenocarcinoma cases. All mesothelioma cases were found to be negative for the other muscle markers examined. We conclude that h-caldesmon is a highly sensitive and specific marker and suggest its inclusion in the immunohistochemical panel for the differential diagnosis of epithelioid mesothelioma versus lung adenocarcinoma.
authors.affiliation: Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy. camilla.comin@unifi.it
authors.forename: Camilla E
authors.initials: CE
authors.lastname: Comin
authors.forename: Sergio
authors.initials: S
authors.lastname: Dini
authors.forename: Luca
authors.initials: L
authors.lastname: Novelli
authors.forename: Raffaella
authors.initials: R
authors.lastname: Santi
authors.forename: Grazia
authors.initials: G
authors.lastname: Asirelli
authors.forename: Luca
authors.initials: L
authors.lastname: Messerini
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000175:diagnosis; D014408:Biomarkers, Tumor / Q000032:analysis; D002148:Calmodulin-Binding Proteins / Q000032:analysis*; D003937:Diagnosis, Differential; D015622:Epithelioid Cells / Q000737:chemistry / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry / Q000175:diagnosis; D008654:Mesothelioma / Q000737:chemistry / Q000473:pathology*; D010997:Pleural Neoplasms / Q000737:chemistry / Q000473:pathology*; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D002148:Calmodulin-Binding Proteins