title: [Estrogenic effects of gasoline-fueled vehicle exhausts and methanol-fueled vehicle exhausts].
abstract: OBJECTIVE
  To investigate the estrogenic effects of gasoline-fueled vehicle exhausts and methanol-fueled vehicle exhausts and provide a scientific basis for replacement of gasoline by methanol as fuel in vehicle.
  
  
  METHODS
  The estrogen-like activities of gasoline-fueled vehicle exhausts and methanol-fueled vehicle exhausts were detected using the recombinant yeast estrogen system.
  
  
  RESULTS
  The A600 value of gasoline-fueled vehicle exhausts was 0. 081 when the concentration of gasoline-fueled vehicle exhausts was 1000 mL/mL, and there were significantly statistical differences as compared with those of the dimethyl sulfoxide (DMSO) control group (P < 0.05), which indicated that such concentration had poisonous effects on yeast cell; when the concentration of gasoline-fueled vehicle exhausts was 31.25 mL/mL, the estrogen-like activities of gasoline-fueled vehicle exhausts could be detected, and there were significantly statistical differences as compared with those of the DMSO control group (P<0.05). Besides, the estrogen-like activities could not be detected in other groups with various concentrations. The estrogen-like activities of methanol-fueled vehicle exhausts could not be detected, and there were no significantly statistical differences in A600 value (P > 0.05) when each group was compared with the DMSO control group.
  
  
  CONCLUSIONS
  Gasoline-fueled vehicle exhausts have weak estrogenic effects, whereas methanol-fueled vehicle exhausts have no such effects.
authors.affiliation: School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Zun-Zhen
authors.initials: ZZ
authors.lastname: Zhang
authors.forename: Cong-Mao
authors.initials: CM
authors.lastname: Ye
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000397:Air Pollution / Q000517:prevention & control; D047628:Estrogen Receptor alpha / Q000187:drug effects* / Q000235:genetics; D004967:Estrogens / Q000032:analysis*; D005742:Gasoline / Q000009:adverse effects / Q000032:analysis*; D006801:Humans; D000432:Methanol / Q000009:adverse effects / Q000032:analysis* / Q000737:chemistry; D001335:Vehicle Emissions / Q000032:analysis*; D015003:Yeasts / Q000187:drug effects / Q000235:genetics; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D004967:Estrogens; D005742:Gasoline; D001335:Vehicle Emissions; D001616:beta-Galactosidase; D000432:Methanol