[Viral transactivation of transcription by NF-kappaB in steroid-responsive simple nephrotic syndrome].
OBJECTIVE
To investigate the path of viral transactivation of transcription by nuclear factor-kappa B (NF-kappaB) in the pathogenesis of steroid-responsive simple nephrotic syndrome (SRSNS) triggered by respiratory tract viruses.


METHODS
Children with SRSNS (including active stage, convalescent and remissive stage) were examined and were analytically compared to children with nephritic nephrosis, secondary glomerular diseases and bronchiolitis; healthy children were enrolled as controls. The methods of electrophoretic mobility shift assay (EMSA), of reverse transcription-PCR (RT-PCR), and of enzyme-linked immunosorbent assay (ELISA), were used to detect the transcriptive activity of NF-kappaB, the gene expression of respiratory tract viruses (respiratory syncytial virus-RSV, influenza virus-Flu) in peripheral blood mononuclear cells (PBMC), and the levels of IL-8 and viral antibodyies in serum, respectively.


RESULTS
(1) Compared with the control and SRSNS in convalescent and remissive stage, the activity of NF-kappaB in PBMC of SRSNS in active stage was statistically elevated (P<0.05). (2)The level of IL-8 in serum of SRSNS in active stage was significantly increased, compared with healthy control (P<0.001). There was a positive linear correlation between the activity of NF-kappaB and the level of IL-8 in active stage of SRSNS, and a positive correlation trend was observed between the activity of NF-kappaB and the gene expression of respiratory tract viruses.


CONCLUSIONS
Viral transactivation of transcription by NF-kappaB may be an important path for respiratory tract viruses triggering the pathogensis of SRSNS.
Liu
Xiao-Ming
XM
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Wang
Zheng
Z
Li
Chan-Sheng
CS
D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D016328:NF-kappa B / Q000097:blood*; D009404:Nephrotic Syndrome / Q000097:blood / Q000821:virology*; D009975:Orthomyxoviridae / Q000235:genetics*; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D014158:Transcription, Genetic
D016209:Interleukin-8; D016328:NF-kappa B