Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section.
Received October 14, 2005; revised January 7, 2006; accepted January 9, 2006; posted January 12, 2006 (Doc. ID 65391) The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wavelength in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.
Tsai
Tsung-Han
TH
Graduate Institute of Electro-Optical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Lin
Cheng-Yung
CY
Tsai
Huai-Jen
HJ
Chen
Szu-Yu
SY
Tai
Shih-Peng
SP
Lin
Kung-Hsuan
KH
Sun
Chi-Kuang
CK
D000818:Animals; D001704:Biopolymers / Q000378:metabolism; D006321:Heart / Q000196:embryology*; D007089:Image Enhancement / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D014018:Tissue Distribution; D015027:Zebrafish; D000097573:Red Fluorescent Protein
D001704:Biopolymers; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein