title: Colonic electrical stimulation regulates colonic transit via the nitrergic pathway in rats.
abstract: Gastrointestinal electrical stimulation has been proposed for the treatment of gastrointestinal motor disorders. However, little is known about potential roles of colonic electrical stimulation (CES). The aim of this study was to evaluate the effect and mechanism of CES on colonic transit in conscious rats. Male rats (N = 14) were equipped with a pair of colonic serosal electrodes for stimulation and a catheter in the colon. Colonic transit was assessed in four randomized sessions with or without CES and with or without nitric oxide synthesis blocker, L-NNA, by calculating the output of phenol red from the anus every 10 min for 90 min. Results were as follows. (1) CES with trains of short pulses significantly enhanced colonic transit. Colonic emptying was 57.3 +/- 6.1% in the control session and 81.9 +/- 4.6% with CES at 90 min, reflecting a 43% increase. (2) L-NNA delayed colonic transit compared with saline and prevented the accelerative effect of CES on colonic transit. We conclude that CES has an excitatory effect on colonic transit and this excitatory effect may be mediated via the nitrergic pathway.
authors.affiliation: Division of Gastroenterology, UniversitTexas Medical Branch, Galveston, Texas, USA.
authors.forename: Shi
authors.initials: S
authors.lastname: Liu
authors.forename: J D Z
authors.initials: JD
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D004558:Electric Stimulation / Q000379:methods*; D004567:Electrodes, Implanted; D005772:Gastrointestinal Transit / Q000187:drug effects / Q000502:physiology*; D043183:Irritable Bowel Syndrome / Q000201:enzymology / Q000628:therapy; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011336:Probability; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012680:Sensitivity and Specificity
chemical_list: D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester