Lipid peroxidation and HSP72/73 expression in rat following cadmium chloride administration: interactions of magnesium supplementation.
OBJECTIVE
to determine whether magnesium (Mg) supplementation could have a protective effect against the cadmium (Cd)-induced oxidative stress in liver, kidneys and testes of adult male rats. Stress was evaluated by measuring lipid peroxidation by thiobarbituric acid reactive substances (TBARS) and the heat shock protein (HSP) 72/73 expression. CdCl2 injections (2.5mg/day/kg body weight) for 10 days resulted in a time dependent increase of Cd accumulation in liver, kidney and testes, the highest levels being found in liver (400 microg/g dried tissue). At the same time, an increase of lipid peroxidation was observed. The effect was maximal at day 1 of Cd treatment in liver and testes, and later (day 5) in kidney. Then, Cd-induced lipid peroxidation decreased, suggesting the activation of antioxidant defense mechanisms. Injections of Mg SO4 (300-600 mg/day/kg body weight) reduced in a dose-dependent manner Cd-induced lipid peroxidation in liver and kidney as well as the accumulation of Cd in liver, kidney and testes. In testes, a protective effect of Mg was found only during the early phase of Cd-poisoning. On days 5 and 10, lipid peroxidation was even increased as compared to controls. In liver and testes only the constitutive HSP73 was detected whereas in kidney both HSP73 and the inducible HSP72 were expressed. HSP72/73 expression was not significantly increased by Cd and HSP73 was even lowered in kidney, probably due to the strong dose used. These results were not modified by Mg injections.


CONCLUSIONS
Mg supplementation can reduce Cd accumulation in organs and lipid peroxidation related to Cd administration.
Boujelben
Manel
M
Laboratoire d'Ecophysiologie Animale de la Faculté des Sciences de Sfax, BP. 802-3018 Sfax, Tunisie.
Ghorbel
Fatma
F
Vincent
Christian
C
Makni-Ayadi
Fatma
F
Guermazi
Fadhel
F
Croute
Françoise
F
El-Feki
Abelfettah
A
D000818:Animals; D019256:Cadmium Chloride / Q000493:pharmacokinetics / Q000633:toxicity*; D002274:Carcinogens, Environmental / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D050884:HSP72 Heat-Shock Proteins / Q000187:drug effects* / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008274:Magnesium / Q000493:pharmacokinetics / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D002274:Carcinogens, Environmental; D050884:HSP72 Heat-Shock Proteins; D017392:Thiobarbituric Acid Reactive Substances; D008274:Magnesium; D019256:Cadmium Chloride