title: Allele and genotype frequencies of polymorphic FMO3 gene in two genetically distinct populations.
abstract: The aims of this study were to analyze flavin-containing monooxygenase 3 (FMO3) polymorphisms and allele and genotype frequencies in 256 Han Chinese and 50 African-American individuals, to compare the allele and genotype frequencies of these populations with those of other world populations. For Han Chinese, genotyping of three common single nucleotide polymorphisms, E158K, V257M and E308G was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). For African-Americans, genotyping of all coding exons was performed by modified PCR-single strand conformational polymorphism (SSCP). Evolutionary rates of FMO3 were estimated computationally. We found that there were significant differences in allele and genotype frequencies among Han Chinese, African-Americans and other world populations. In Han Chinese, the minor allele frequencies (MAFs) were 0.229 (E158K), 0.203 (V257M) and 0.148 (E308G), respectively. In African-Americans, MAFs were 0.48 (E158K), 0.05 (V257M) and 0 (E308G), respectively. There was rapid evolution during the divergence of primate FMO3. This is the first report comparing FMO alleles and genotypes between Han Chinese and African-Americans. A Han Chinese population database has been established for three gene polymorphisms. The data presented here justify further pharmacogenetic studies for potentially optimizing recommended drug dosages and evaluating relationships with disease processes.
authors.affiliation: Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: DaCheng
authors.initials: D
authors.lastname: Hao
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Bjarte
authors.initials: B
authors.lastname: Furnes
authors.forename: Daniel
authors.initials: D
authors.lastname: Schlenk
authors.forename: MiaoXin
authors.initials: M
authors.lastname: Li
authors.forename: ShengLi
authors.initials: S
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
mesh_terms: D000328:Adult; D001741:Black or African American / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D002681:China; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010105:Oxygenases / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide
chemical_list: D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming)