Molecular characterization of polyphosphate (PolyP) operon from Serratia marcescens.
The polyphosphate (polyP) operon was cloned from a genomic library of Serratia marcescens KCTC 2172 by Southern hybridization using E. coli ppk gene as a probe. The polyP operon was composed of a polyphosphate promoter, polyphosphate kinase (ppk) and exopolyphosphatase (ppx). A potential CRP binding site and pho box sequence were found in the region upstream of the putative promoter in the regulatory region. The ppk gene comprises 2,063 nucleotides and encodes 686 amino acids yielding a protein with a molecular mass of 70 kDa. The ppx gene contains 1611 nucleotides and encodes 536 amino acids with a molecular 58 kDa. An E. coli strain transformed with the ppk gene had a 16-fold increased in polyphosphate kinase activity, while introduction of the ppx gene produced a 25-fold increase in polyphosphatase activity. E. coli strains transformed with ppk and ppx genes also displayed increased accumulation of polyphosphate.
Lee
Seung-Jin
SJ
Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University, Busan, 604-714, South Korea.
Lee
Yong-Seok
YS
Lee
Young-Choon
YC
Choi
Yong-Lark
YL
D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics; D015698:Genomic Library; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D009876:Operon; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics; D011122:Polyphosphates / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020543:Proteome / Q000302:isolation & purification; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D012706:Serratia marcescens / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D011122:Polyphosphates; D020543:Proteome; D017853:Phosphotransferases (Alcohol Group Acceptor); D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase; C475691:polyphosphate kinase, E coli; D010749:Phosphoprotein Phosphatases; C080406:protein phosphatase 4