title: Evaluation of antigen panels for ELISA monitoring of mouse colonies for antibodies to Pasteurellaceae.
abstract: Pasteurellaceae infection in mice may be monitored by the detection of serum antibody using enzyme-linked immunosorbent assay (ELISA). We re-evaluated our standard antigen panel comprising Pasteurella pneumotropica and a V-factor requiring Haemophilus species (strain H21) by studying their serological relationship with Actinobacillus muris and 'Haemophilus influenzae-murium'. Serologically, A. muris and 'H. influenzae-murium' were found to be unrelated and to differ from P. pneumotropica and Haemophilus strain H21. These four antigens were used for monitoring breeding and experimental mouse colonies for a period of four years. The addition of 'H. influenzae-murium' antigen to the standard panel of antigens significantly increased the proportion of sera and serum panels showing anti-Pasteurellaceae antibody activity, but the addition of A. muris antigen did not.
authors.affiliation: Section of Laboratory Animal Microbiology, Diagnostic Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. r.boot@rivm.nl
authors.forename: R
authors.initials: R
authors.lastname: Boot
authors.forename: L
authors.initials: L
authors.lastname: van de Berg
mesh_terms: D000818:Animals; D000830:Animals, Laboratory; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000737:chemistry*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D051379:Mice / Q000097:blood / Q000276:immunology*; D015661:Pasteurellaceae / Q000276:immunology / Q000302:isolation & purification*; D016871:Pasteurellaceae Infections / Q000097:blood / Q000662:veterinary*; D012376:Rodent Diseases / Q000097:blood / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial