[Effects of temperature on the kinetics and level of energy charge and oxidation-reduction state in pyruvate biosynthesis].
Temperature plays an important role in pyruvate biosynthesis by Torulopsis glabrata 620. The effects of temperature on the substrate consumption, cell growth, pyruvate biosynthesis and level of energy charge and oxidation-reduction state have been investigated. During the constant temperature fermentation, higher temperature can enhance the rate of glucose consumption, cell growth and pyruvate production. However, it also leads to higher energy charge in the prophase of fermentation, too much consumption of glucose by cell and deficient throughput of pyruvate in the anaphase of fermentation, which brings on decrease of pyruvate yield. Oppositely, maintaining lower temperature during the fermentation can offer constant production capacity of pyruvate in the anaphase of fermentation. But the disadvantages are lower cell growth and higher level of NADH/NAD+ in vivo during the anaphase of fermentation, which leads to lower productivity inevitably. Disaccords above-mentioned indicate that the higher production, higher yield and higher productivity cannot be achieved at one time during a constant temperature fermentation.
Wang
Cui-Hua
CH
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Li
You-Yuan
YY
Chen
Chang-Hua
CH
Li
Xiao
X
D041221:Candida glabrata / Q000254:growth & development / Q000378:metabolism*; D004734:Energy Metabolism; D005285:Fermentation; D007700:Kinetics; D010084:Oxidation-Reduction; D019289:Pyruvic Acid / Q000378:metabolism*; D013696:Temperature
D019289:Pyruvic Acid