Drug monitoring and toxicology: a procedure for the monitoring of oxcarbazepine metabolite by HPLC-UV.
This article describes a rapid high-performance liquid chromatographic (HPLC) method for the measurement of the primary metabolite of oxcarbazepine. Following a simple precipitation step, 10,11,-dihydro-10-hydroxy-5H-dibenzo(b,f)azepine-5-carboxamide is quantitated (5-60 microg/mL) by analysis on an HPLC-UV system. The instrument time is less than 5 min per injection, an improvement over most published methods. The assay's limit of quantitation, linearity, imprecision, and accuracy adequately cover the therapeutic range for appropriate patient monitoring. In comparison to other published methods, this procedure would be of interest to clinical laboratories because it employs a precipitation step for sample preparation, instead of conventional yet time-consuming solid-phase extraction.
Juenke
Joetta M
JM
ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories Inc, Salt Lake City, Utah 84108, USA. juenkejm@aruplab.com
Brown
Paul I
PI
Urry
Francis M
FM
McMillin
Gwendolyn A
GA
D000927:Anticonvulsants / Q000097:blood* / Q000633:toxicity; D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000097:blood / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring; D006801:Humans; D000078330:Oxcarbazepine; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000927:Anticonvulsants; D002220:Carbamazepine; D000078330:Oxcarbazepine