title: Use of enzyme-linked immunosorbent assay for detection of natural leishmania infections in phlebotomine sand flies from southeastern Turkey.
abstract: In total, 320 phelebotomine sand flies from Alibozlu (Osmaniye), Kizyusuflu (Osmaniye), and Sanliurfa in southeastern Turkey were tested for the detection and identification of Leishmania in vector sand flies by enzyme-linked immunosorbent assay with species-specific monoclonal antibodies. We used monoclonal antibodies that recognize both Leishmania tropica and Leishmania major, and a monoclonal antibody specific only to L. tropica. Phosphate-buffered saline and monoclonal antibody M2 recognizing Leishmania amazonensis were used as controls. Infection rates of sand flies were 0.9% in Alibozlu, 0% Kizyusuflu, and 3.6% in Sanliurfa. Positive sand flies were identified as Phlebotomus sergenti Parrot and Phlebotomus major syriacus Adler & Theodor.
authors.affiliation: Department of Medical Biology and Genetics, School of Medicine, University of Cukurova, 01330 Balcali, Adana, Turkey.
authors.forename: Ayfer
authors.initials: A
authors.lastname: Pazarbasi
authors.forename: Davut
authors.initials: D
authors.lastname: Alptekin
authors.forename: H Umit
authors.initials: HU
authors.lastname: Luleyap
authors.forename: Mulkiye
authors.initials: M
authors.lastname: Kasap
authors.forename: Halil
authors.initials: H
authors.lastname: Kasap
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D007303:Insect Vectors / Q000145:classification / Q000469:parasitology*; D007891:Leishmania / Q000276:immunology* / Q000302:isolation & purification*; D018320:Leishmania major / Q000302:isolation & purification; D007895:Leishmania tropica / Q000302:isolation & purification; D010691:Phlebotomus / Q000145:classification / Q000469:parasitology*; D015995:Prevalence; D013045:Species Specificity; D014421:Turkey
chemical_list: D000913:Antibodies, Protozoan