[The effects of different transcripts of AML1 on the transactivation of M-CSF-R gene].
OBJECTIVE
To observe the effects of AML1A and AML1B, two splicing isoforms of AML1, on the transactivation of macrophage colony-stimulating factor receptor (M-CSF-R), and explore the mechanism of hematopoietic stem cell committed differentiation and leukemogenesis.


METHODS
The expressive plasmids of AML1A and AML1B were constructed, and co-transfected into CV-1 cells with a luciferase reporter plasmid containing M-CSF-R promoter. The transactivity of M-CSF-R promoter was assayed by luminometer.


RESULTS
AML1B exhibited a distinct transactivity to M-CSF-R promoter with a sequence-specificity and dosage-dependent manner. AML1A showed no any transactivity but antagonized the effect of AML1B, causing marked reduction of M-CSF-R expression.


CONCLUSIONS
An intact structure of AML1 is necessary for transactivation of M-CSF-R. AML1A may interfere with the transactivation of AML1B, and play a key role in the fine regulation of committed differentiation of hematopoietic cell.
Zhang
Qing
Q
Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
Wang
Min
M
Xing
Hai-yan
HY
Rao
Qing
Q
Wang
Jian-xiang
JX
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic; D005822:Genetic Vectors; D000882:Haplorhini; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007668:Kidney / Q000166:cytology; D010957:Plasmids / Q000235:genetics; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection
D050676:Core Binding Factor Alpha 2 Subunit; D016186:Receptor, Macrophage Colony-Stimulating Factor