title: Central nervous system stem/progenitor cells form neurons and peripheral glia after transplantation to the dorsal root ganglion.
abstract: We asked whether neural stem/progenitor cells from the cerebral cortex of E14.5 enhanced green fluorescent protein transgenic mice are able to survive grafting and differentiate in the adult rat dorsal root ganglion. Neurospheres were placed in lumbar dorsal root ganglion cavities after removal of the dorsal root ganglia. Alternatively, dissociated neurospheres were injected into intact dorsal root ganglia. Enhanced green fluorescent protein-positive cells in the dorsal root ganglion cavity were located in clusters and expressed beta-III-tubulin or glial fibrillary acidic protein after 1 month, whereas after 3 months, surviving grafted cells expressed only glial fibrillary acidic protein. In the intact adult DRG, transplanted neural stem/progenitor cells surrounded dorsal root ganglion cells and fibers, and expressed glial but not neuronal markers. These findings show that central nervous system stem/progenitor cells can survive and differentiate into neurons and peripheral glia after xenotransplantation to the adult dorsal root ganglion.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University Biomedical Center, Uppsala, Sweden. karin.brannvall@imbim.uu.se
authors.forename: Karin
authors.initials: K
authors.lastname: Brännvall
authors.forename: Martin
authors.initials: M
authors.lastname: Sandelin
authors.forename: Ulrika
authors.initials: U
authors.lastname: Wallenquist
authors.forename: Karin
authors.initials: K
authors.lastname: Forsberg-Nilsson
authors.forename: Håkan
authors.initials: H
authors.lastname: Aldskogius
authors.forename: Elena N
authors.initials: EN
authors.lastname: Kozlova
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology; D004622:Embryo, Mammalian; D005727:Ganglia, Spinal / Q000166:cytology* / Q000637:transplantation; D015870:Gene Expression / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000502:physiology*; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
chemical_list: D000199:Actins; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; C577486:Nes protein, rat; D064231:Nestin; D014404:Tubulin; D049452:Green Fluorescent Proteins