Identification and classification of genes that act antagonistically to let-60 Ras signaling in Caenorhabditis elegans vulval development.
The synthetic multivulva (synMuv) genes negatively regulate Ras-mediated vulval induction in the nematode Caenorhabditis elegans. The synMuv genes define three classes, A, B, and C, such that double mutants carrying mutations in genes of any two classes are multivulva. The class B synMuv genes include lin-35, a homolog of the retinoblastoma (Rb) tumor suppressor gene, as well as homologs of genes that function with Rb in transcriptional regulation. We screened for additional synMuv mutations using a strategy different from that of previous synMuv genetic screens. Some of the mutations we recovered affect new synMuv genes. We present criteria for assigning synMuv mutations into different genetic classes. We also describe the molecular characterization of the class B synMuv gene lin-65.
Ceol
Craig J
CJ
Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Stegmeier
Frank
F
Harrison
Melissa M
MM
Horvitz
H Robert
HR
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D003433:Crosses, Genetic; D018509:DNA, Helminth / Q000235:genetics; D005260:Female; D017238:Genes, Helminth; D008040:Genetic Linkage; D006579:Heterozygote; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D015398:Signal Transduction; D014844:Vulva / Q000254:growth & development; D018631:ras Proteins / Q000235:genetics* / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D018509:DNA, Helminth; C066646:let-60 protein, C elegans; D018631:ras Proteins