Isolation and antifungal and antioomycete activities of staurosporine from Streptomyces roseoflavus strain LS-A24.
The actinomycete strain LS-A24 active against some plant fungal and oomycete pathogens was isolated from a soil sample of the Sunghwan Lake in Korea. The cell wall composition and spore shape of strain LS-A24 were LL-diaminopimelic acid and spiral type, respectively. On the basis of the physiological and biochemical characteristics and 16S ribosomal DNA sequence analysis, strain LS-A24 was identical to Streptomyces roseoflavus. An antifungal and antioomycete antibiotic was isolated from LS-A24 using various chromatographic procedures. The molecular formular of the antibiotic was determined to be C(28)H(26)N(4)O(3), and on the basis of the NMR data, the antibiotic was confirmed to be staurosporine, 2,3,10,11,12,13-hexahydro-10R-methoxy-9S-methyl-11R-methylamino-9S,13R-epoxy-1H,9H-diindolo[1,2,3-gh:3',2',1'-lm]pyrrolo[3,4-j][1,7]benzodiazonin-1-one. Staurosporine completely inhibited the mycelial growth of Colletotrichum orbiculare, Phytophthora capsici, Rhizoctonia solani, Botrytis cinerea, and Cladosporium cucumerinum with minimum inhibitory concentration (MIC) values of 1-50 microg/mL for MICs. Staurosporine also was active against Saccharomyces cerevisiae, Bacillus subtilis ssp. subtilis, and Xanthomonas vesicatoria. Staurosporine and the commercial fungicide metalaxyl inhibited the development of Phytophthora blight on pepper plants. However, the control efficacy of staurosporine against the Phytophthora disease was somewhat less than that of metalaxyl. This is the first study to isolate staurosporine from S. roseoflavus and demonstrate its in vitro and in vivo antioomycete activity against P. capsici.
Park
Hee Jin
HJ
Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul 136-713, Korea.
Lee
Jung Yeop
JY
Hwang
In Sun
IS
Yun
Bong Sik
BS
Kim
Beom Seok
BS
Hwang
Byung Kook
BK
D005659:Fungicides, Industrial / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D009868:Oomycetes / Q000187:drug effects*; D010802:Phylogeny; D010944:Plants / Q000382:microbiology; D012988:Soil Microbiology; D019311:Staurosporine / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification
D005659:Fungicides, Industrial; D019311:Staurosporine