Locked nucleic acid: high-affinity targeting of complementary RNA for RNomics.
Locked nucleic acid (LNA) is a nucleic acid analog containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA-mimicking sugar conformation. This conformational restriction is translated into unprecedented hybridization affinity towards complementary single-stranded RNA molecules. That makes fully modified LNAs, LNA/DNA mixmers, or LNA/RNA mixmers uniquely suited for mimicking RNA structures and for RNA targeting in vitro or in vivo. The focus of this chapter is on LNA antisense, LNA-modified DNAzymes (LNAzymes), LNA-modified small interfering (si)RNA (siLNA), LNA-enhanced expression profiling by real-time RT-PCR and detection and analysis of microRNAs by LNA-modified probes.
Kauppinen
S
S
Department of Functional Genomics, Exiqon, Bygstubben 9, 2950 Vedbaek, Denmark.
Vester
B
B
Wengel
J
J
D000818:Animals; D006801:Humans; D009696:Nucleic Acids / Q000737:chemistry* / Q000187:drug effects*; D012313:RNA / Q000187:drug effects; D016372:RNA, Antisense / Q000494:pharmacology; D018075:RNA, Complementary / Q000187:drug effects*; D034741:RNA, Small Interfering / Q000494:pharmacology
D009696:Nucleic Acids; D016372:RNA, Antisense; D018075:RNA, Complementary; D034741:RNA, Small Interfering; D012313:RNA