Amino acid substitutions in malate dehydrogenases of piezophilic bacteria isolated from intestinal contents of deep-sea fishes retrieved from the abyssal zone.
To examine the occurrence in other deep-sea bacteria of two amino acid substitutions (Ala-180 and His-229) in malate dehydrogenase (MDH) found previously in the deep-sea piezophilic Moritella sp. strain 2D2, we cloned and sequenced MDH genes of deep-sea piezophilic Moritella and Shewanella strains isolated from intestinal contents of deep-sea fishes, as well as other Moritella species from deep-sea water and sediments: M. marina, M. japonica, and M. yayanosii. The piezophilic Moritella strains had a Val residue or an Ala residue at position 180 and all the Moritella strains except for one had a His residue at position 229. However, four piezophilic-strain-specific substitutions at positions 103, 111, 229, and 283 were found to be completely conserved in the MDH of the intestinal Moritella strains of deep-sea fishes, indicating the substitutions may be habitat-specific. The piezophilic Shewanella strains had a Val residue and a Gln residue at positions 180 and 229, respectively. However, the MDHs of the Shewanella strains had five piezophilic-strain-specific substitutions at positions 61, 65, 107, 161, and 202. Therefore, the enzymatic strategies for responding to deep-sea high pressure environments of the MDHs between the genera Moritella and Shewanella are potentially different. Moreover, homology modeling shows these substitutions found in the MDHs of both genera except for position 229 in the subunit interface are located on the exposed region of the MDH molecules, indicating the substitutions may be related to the hydration state of the molecules.
Saito
Rie
R
Department of Fisheries, Faculty of Agriculture, Kinki University, Nakamachi, Nara, Japan.
Kato
Chiaki
C
Nakayama
Akihiko
A
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005399:Fishes / Q000382:microbiology*; D005766:Gastrointestinal Contents / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology; D008291:Malate Dehydrogenase / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D034221:Moritella / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D012623:Seawater / Q000382:microbiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020592:Shewanella / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D014871:Water Microbiology
D004269:DNA, Bacterial; D008291:Malate Dehydrogenase