title: Comparison of TEST 1 with SRS 100 and ICSH reference method for the measurement of the length of sedimentation reaction in blood.
abstract: BACKGROUND
  We evaluated the measurement of length of sedimentation reaction in blood (LSRB) by TEST 1 and compared the results with those for the Westergren and Sed Rate Screener 100 (SRS 100) methods.
  
  
  METHODS
  LSRB was measured in 113 paired blood samples.
  
  
  RESULTS
  TEST 1 correlated significantly with the Westergren (r=0.94) and SRS 100 (r=0.90) methods with low bias (-0.29 and -1.92 mm/h, respectively) and limits of agreement (-14.5 to 13.9, and -23.4 to 19.6 mm/h, respectively). Hematocrit (Htc) correlated negatively with LSRB in TEST 1 (r=-0.54) and SRS 100 (r=-0.53) only in samples with high Htc (>/=35%). The bias and limits of agreement between TEST 1 and Westergren in samples with low (-1.46 and -22.3 to 19.3 mm/h) and high (0.43 and -7.29 to 8.14 mm/h) Htc were comparable to those between SRS 100 and Westergren (1.83 and -27.2 to 30.9 mm/h for low, 0.71 and -7.27 to 8.70 mm/h for high Htc samples). Total protein and fibrinogen correlated similarly with LSRB in both TEST 1 (r=0.23 and 0.48, respectively) and SRS 100 (r=0.30 and 0.51, respectively).
  
  
  CONCLUSIONS
  The findings suggested that TEST 1 is a reliable, precise and accurate system for measurement of LSRB in clinical laboratories with high workload.
authors.affiliation: Clinical Biochemistry Unit, Medical Faculty, Central Laboratory, Akdeniz University, Antalya, Turkey. ozdem@akdeniz.edu.tr
authors.forename: Sebahat
authors.initials: S
authors.lastname: Ozdem
authors.forename: Halide S
authors.initials: HS
authors.lastname: Akbas
authors.forename: Levent
authors.initials: L
authors.lastname: Donmez
authors.forename: Meral
authors.initials: M
authors.lastname: Gultekin
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis; D001799:Blood Sedimentation; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006400:Hematocrit; D006801:Humans; D008297:Male; D008722:Methods; D008875:Middle Aged; D011446:Prospective Studies; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D001798:Blood Proteins; D005340:Fibrinogen