title: Chronic intake of caffeine during gestation down regulates metabotropic glutamate receptors in maternal and fetal rat heart.
abstract: Caffeine is the most widely consumed substance in the world which antagonizes adenosine effects. Adenosine acting through A(1) receptors inhibits glutamate release which binds to metabotropic glutamate receptors (mGluRs). Recently, we have shown that maternal caffeine intake during gestation causes down-regulation of A(1) and metabotropic glutamate receptors in the brain of both rat mothers and fetuses. In the present work we provide evidence that caffeine also affects receptors in hearts, causing a decrease in mGluRs from both maternal and fetal hearts. A decrease in G(q/11) and PLC beta(1) proteins level was also observed in both tissues. However, phospholipase C activity was only affected in fetal heart, being significantly decreased. These results suggest an in vivo cross-talk mechanism between adenosine and glutamate receptors in peripheral tissues. Therefore, special attention should be paid to caffeine ingestion during gestation.
authors.affiliation: Department of Inorganic, Organic and Biochemistry, Faculty of Chemical Sciences, Centro Regional de Investigaciones Biomédicas, Ciudad Real, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Iglesias
authors.forename: D
authors.initials: D
authors.lastname: León
authors.forename: M A
authors.initials: MA
authors.lastname: Ruiz
authors.forename: J L
authors.initials: JL
authors.lastname: Albasanz
authors.forename: M
authors.initials: M
authors.lastname: Martín
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology; D002110:Caffeine / Q000494:pharmacology*; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects*; D005260:Female; D005318:Fetal Heart / Q000378:metabolism*; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D007527:Isoenzymes / Q000378:metabolism; D008431:Maternal-Fetal Exchange / Q000187:drug effects; D054799:Phospholipase C beta; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D043682:Receptor, Adenosine A1 / Q000378:metabolism; D018094:Receptors, Metabotropic Glutamate / Q000096:biosynthesis*; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D000697:Central Nervous System Stimulants; D007527:Isoenzymes; D043682:Receptor, Adenosine A1; D018094:Receptors, Metabotropic Glutamate; D002110:Caffeine; D010738:Type C Phospholipases; D054799:Phospholipase C beta; C518035:Plcb1 protein, rat; D043802:GTP-Binding Protein alpha Subunits, Gq-G11