title: T-reg mediated suppression of the allograft response in the draining lymph node.
abstract: We previously demonstrated that T-regs inhibit proliferation of graft-reactive T cells in the draining lymph node (DLN), suggesting that this site may be important for regulation. TCR transgenic mice (TS1) specific for viral hemagglutinin (HA) provided antigen-specific T cells for adoptive transfer into syngeneic Balb/c hosts bearing HA+ skin grafts. T-regs were obtained from (TS1xHA28)F1 mice known to have an expanded population of HA-specific T-regs. To determine whether the lymph node is an independent site of suppression, we developed a model in which donor antigen that migrates from the allograft to the DLN drives T-cell activation after graft removal. T-regs that did not encounter the allograft itself remained able to inhibit graft antigen-specific T-cell proliferation in the DLN. Alloantigen-induced regulation can occur in the absence of the graft. This finding identifies the DLN as a potentially critical site of regulation in the early posttransplant period.
authors.affiliation: Department of Surgery, Hospital of the University of Pennsylvania, PA 19104, USA.
authors.forename: Major K
authors.initials: MK
authors.lastname: Lee
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Moore
authors.forename: Meredith
authors.initials: M
authors.lastname: Chiaccio
authors.forename: Moh Moh
authors.initials: MM
authors.lastname: Lian
authors.forename: Shaoping
authors.initials: S
authors.lastname: Deng
authors.forename: Muhammad
authors.initials: M
authors.lastname: Mohiuddin
authors.forename: Xiaolun
authors.initials: X
authors.lastname: Huang
authors.forename: Brigitte
authors.initials: B
authors.lastname: Koeberlein
authors.forename: Adam
authors.initials: A
authors.lastname: Zakheim
authors.forename: Paige M
authors.initials: PM
authors.lastname: Porrett
authors.forename: Clyde F
authors.initials: CF
authors.lastname: Barker
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Caton
authors.forename: James F
authors.initials: JF
authors.lastname: Markmann
mesh_terms: D000818:Animals; D007165:Immunosuppression Therapy; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D015375:Receptors, Interleukin-2 / Q000097:blood; D016038:Skin Transplantation / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D023001:Transplantation Tolerance / Q000276:immunology; D014184:Transplantation, Homologous / Q000276:immunology*
chemical_list: D015375:Receptors, Interleukin-2