title: Binding behaviour of pyrimethanil-imprinted polymers prepared in the presence of polar co-monomers.
abstract: Here, we report the evaluation of the molecular recognition properties of a small library of pyrimethanil-imprinted pellicular beads prepared in the presence of several co-monomers of decreasing hydrophobicity, namely N-methacrylamidopiperidine, methylmethacrylate, N-methacrylamidopyrrolidine, N-vinylpyrrolidone, 2-hydroxyethylmethacrylate, acrylonitrile, N-methacrylamidomorpholine, and N,N-dimethylacrylamide. The effect of the presence of these co-monomers on the molecular recognition properties of the beads were studied by eluting the pyrimethanil/Sudan Orange G pair, in the presence of acetonitrile/water mobile phases of increasing polarity. The pyrimethanil was used to probe the specific interactions due to the presence of binding sites. The Sudan Orange G (that is greatly different from the template but with comparable hydrophobicity and molecular mass) was instead used to probe the non-specific interactions due to the bulk of the polymer. The experimental results show that the polymers prepared in the presence of polar co-monomers are characterised by a marked increase of the analytes retention if compared with the imprinted polymer prepared without these co-monomers. The increase of retention is more pronounced for pyrimethanil compared to Sudan Orange G with polymers prepared in the presence of the more hydrophilic co-monomers N-vinylpyrrolidone, acrylonitrile and N-methacrylamidomorpholine, while N,N-dimethylacrylamide is the only co-monomer that does not significantly influence the retention of analytes. Increasing the amount of water in the mobile phase progressively diminishes this effect.
authors.affiliation: Laboratory of Bioanalytical Chemistry, Department of Analytical Chemistry, University of Turin, Via Giuria 5, 10125 Turin, Italy. claudio.baggiani@unito.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Baggiani
authors.forename: Patrizia
authors.initials: P
authors.lastname: Baravalle
authors.forename: Cristina
authors.initials: C
authors.lastname: Giovannoli
authors.forename: Cinzia
authors.initials: C
authors.lastname: Tozzi
mesh_terms: D001665:Binding Sites; D002853:Chromatography, Liquid; D011108:Polymers / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*
chemical_list: D011108:Polymers; D011743:Pyrimidines; C108337:pyrimethanil