title: Analysis of aromatase and 17beta-hydroxysteroid dehydrogenase type 2 messenger ribonucleic acid expression in deep endometriosis and eutopic endometrium using laser capture microdissection.
abstract: OBJECTIVE
  To investigate mRNA expression of aromatase and 17beta-hydroxysteroid dehydrogenase type 2 (17betaHSD2) in epithelial and stromal cells from eutopic and ectopic endometrium of patients with deep endometriosis.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Patients with deep endometriosis and fertile women with macroscopically normal pelvic cavities.
  
  
  METHODS
  During surgery, 30 endometrial and 16 endometriotic samples were obtained from 30 patients with deep endometriosis. Control endometrial samples were obtained from 24 fertile women with macroscopically normal pelvic cavities who underwent laparoscopic tubal ligation or reversal of tubal sterilization. Epithelial cells and stromal cells from endometrial or endometriotic tissues were microdissected using laser capture microdissection.
  
  
  METHODS
  Expression levels of aromatase and 17betaHSD2 mRNA in microdissected epithelial and stromal cells were determined using quantitative real-time reverse transcriptase polymerase chain reaction.
  
  
  RESULTS
  Aromatase mRNA expression was significantly higher in epithelial cells than in stromal cells in both eutopic and ectopic endometrium obtained from endometriosis patients. In the ectopic endometrium of 8 patients (8/16, 50%), 17betaHSD2 expression was not detected in either epithelial or stromal cells. In eutopic endometrium from endometriosis patients, 17betaHSD2 expression in epithelial cells was significantly increased during the early, middle, and late secretory phases compared with the late proliferative phase, whereas no significant cyclical difference was detected in control endometrium.
  
  
  CONCLUSIONS
  Local estrogen concentration may be much higher in epithelial cells than in stromal cells in deep endometriotic tissue.
authors.affiliation: Department of Gynecology, Polyclinique de l'Hôtel-Dieu, CHU, Clermont-Ferrand, France. sachikoma@aol.com
authors.forename: Sachiko
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: Michel
authors.initials: M
authors.lastname: Canis
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Pouly
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Déchelotte
authors.forename: Gérard
authors.initials: G
authors.lastname: Mage
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D001141:Aromatase / Q000378:metabolism*; D004715:Endometriosis / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D004717:Endometrium / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D004847:Epithelial Cells / Q000201:enzymology; D004960:Estradiol Dehydrogenases; D005260:Female; D005498:Follicular Phase; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D053685:Laser Therapy; D008183:Luteal Phase; D042282:Microdissection; D011446:Prospective Studies; D012333:RNA, Messenger / Q000378:metabolism*; D017154:Stromal Cells / Q000201:enzymology; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D015067:17-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases; C523763:HSD17B2 protein, human; D001141:Aromatase