Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing.
A novel method for generation of insect-based cell-free translation extracts is presented. The protocol can be completed in less than an hour, and the resulting extracts are extremely proficient in N-linked glycosylation and signal sequence processing. No specialized equipment other than that usually present in an ordinary biochemistry laboratory is required. The novel approach dramatically reduces cost and time while rendering enhanced lysates compared to previously published strategies.
Katzen
Federico
F
Invitrogen Corp, Carlsbad, CA 92008, USA.
Kudlicki
Wieslaw
W
D000818:Animals; D001709:Biotechnology / Q000379:methods; D002457:Cell Extracts / Q000737:chemistry; D002460:Cell Line; D002474:Cell-Free System / Q000378:metabolism; D006031:Glycosylation; D014176:Protein Biosynthesis / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics*; D015203:Reproducibility of Results; D018411:Spodoptera / Q000166:cytology / Q000378:metabolism*
D002457:Cell Extracts; D021382:Protein Sorting Signals