Molecular typing of wine yeast strains Saccharomyces bayanus var. uvarum using microsatellite markers.
The Saccharomyces bayanus var. uvarum yeasts are associated with spontaneous fermentation of must. Some strains were shown to be enological yeasts of interest in different winemaking processes. The molecular typing of S. bayanus var. uvarum at the strain level has become significant for wine microbiologists. Four microsatellite loci were defined from the exploration of genomic DNA sequence of S. bayanus var. uvarum. The 40 strains studied were homozygote for the locus considered. The discriminating capacity of the microsatellite method was found to be equal to that of karyotypes analysis. Links between 37 indigenous strains with the same geographic origin could be established through the analysis of microsatellite patterns. The analysis of microsatellite polymorphism is a reliable method for wine S. bayanus var. uvarum strains and their hybrids with Saccharomyces cerevisiae identification in taxonomic, ecological studies and winemaking applications.
Masneuf-Pomarède
I
I
ENITA de Bordeaux, 1 Cours du Général de Gaulle, BP 201, CS 40201, 33175 Gradignan cedex, France. i-masneuf@enitab.fr
Le Jeune
C
C
Durrens
P
P
Lollier
M
M
Aigle
M
M
Dubourdieu
D
D
D002678:Chimera; D004586:Electrophoresis / Q000379:methods; D016681:Genome, Fungal; D007621:Karyotyping; D018895:Microsatellite Repeats; D016533:Mycological Typing Techniques; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014920:Wine / Q000382:microbiology*