title: Mapping novel traits by array-assisted bulk segregant analysis in Saccharomyces cerevisiae.
abstract: We demonstrate a new method, microarray-assisted bulk segregant analysis, for mapping traits in yeast by genotyping pooled segregants. We apply a probabilistic model to the progeny of a single cross and as little as two microarray hybridizations to reliably map an auxotrophic marker, a Mendelian trait, and a major-effect quantitative trait locus.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Brauer
authors.forename: Cheryl M
authors.initials: CM
authors.lastname: Christianson
authors.forename: Dave A
authors.initials: DA
authors.lastname: Pai
authors.forename: Maitreya J
authors.initials: MJ
authors.lastname: Dunham
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D020090:Chromosome Segregation; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011336:Probability; D040641:Quantitative Trait Loci; D012441:Saccharomyces cerevisiae / Q000235:genetics*