title: Preparation and characterization of solid lipid nanoparticles loaded traditional Chinese medicine.
abstract: Ultrasonication was employed to prepare solid lipid nanoparticles (SLN). The model traditional Chinese medicine, tetrandrine (TET), was incorporated into SLN. The TET-loaded SLN (TET-SLN) were spherical in the photograph of transmission electron microscope (TEM). The particle size measured by laser diffraction (LD) was found to be 157.3+/-8.2nm. Zeta potential analyzer suggested the zeta potential of TET-SLN was -29.36+/-3.68mV in distilled water. The entrapment efficiency (EE%) was determined with the sephadex gel chromatogram and high-performance liquid chromatogram (HPLC), and up to 90.59% of TET was incorporated. Stability evaluation showed relatively long-term stability with only slight particle growth (P>0.05) after storage at room temperature for 4 weeks. Therefore, ultrasonication is demonstrated to be a simple, available and effective method to prepare high quality SLN loaded traditional Chinese medicine.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, PR China. l_y_ch@163.com
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.forename: Ai
authors.initials: A
authors.lastname: Jia
authors.forename: Xinming
authors.initials: X
authors.lastname: Chang
authors.forename: Hui
authors.initials: H
authors.lastname: Xue
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers; D016503:Drug Delivery Systems; D007834:Lasers; D008055:Lipids / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D046529:Microscopy, Electron, Transmission; D008956:Models, Chemical; D015233:Models, Statistical; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D012995:Solubility; D013010:Sonication
chemical_list: D004337:Drug Carriers; D008055:Lipids