title: Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection.
abstract: A simple method for separation of different anionic and zwitterionic phospholipid classes by capillary zone electrophoresis (CZE), using indirect UV detection with adenosine monophosphate (AMP) as background electrolyte and the UV-absorbing additive, was successfully developed in this study. The separation conditions including apparent pH (pH*) of running buffer, concentration of AMP, organic solvent, applied voltage and capillary temperature were systematically optimized. The application of this method to human blood sample was also briefly examined.
authors.affiliation: Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemistry, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Juan
authors.initials: J
authors.lastname: Dong
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Liao
authors.forename: Yiping
authors.initials: Y
authors.lastname: Liao
authors.forename: Huwei
authors.initials: H
authors.lastname: Liu
mesh_terms: D000249:Adenosine Monophosphate / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010743:Phospholipids / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013696:Temperature
chemical_list: D010743:Phospholipids; D012997:Solvents; D000249:Adenosine Monophosphate