title: Methylation status of the Ep-CAM promoter region in human breast cancer cell lines and breast cancer tissue.
abstract: We examined the methylation status of the Ep-CAM promoter region of human breast cancer cell lines and breast cancer tissue using MethyLight technology and bisulfite sequencing. We found the promoter of Ep-CAM-negative breast cancer cell lines Hs 578T to be methylated to a higher degree as compared to positive cell lines MCF-7. Demethylation of cell lines was performed using 5-aza-2'-deoxycytidine. Ep-CAM RNA and protein expression could be partially restored by treating cells with 5-Aza-2'-deoxycytidine. In most primary breast cancer tissue, methylation of the Ep-CAM gene could be detected at a low level and no correlation was found with Ep-CAM protein expression in tumour tissue. Taken together, these data suggest that methylation of the Ep-CAM promoter is not a crucial mechanism for regulation of Ep-CAM expression in breast cancer. Thus, most important regulatory mechanisms have to be supposed in vivo.
authors.affiliation: Division of Haematology and Oncology, Innsbruck Medical University, Innsbruck, Austria. gilbert.spizzo@uibk.ac.at
authors.forename: Gilbert
authors.initials: G
authors.lastname: Spizzo
authors.forename: Guenther
authors.initials: G
authors.lastname: Gastl
authors.forename: Peter
authors.initials: P
authors.lastname: Obrist
authors.forename: Dominic
authors.initials: D
authors.lastname: Fong
authors.forename: Margot
authors.initials: M
authors.lastname: Haun
authors.forename: Kurt
authors.initials: K
authors.lastname: Grünewald
authors.forename: Walther
authors.initials: W
authors.lastname: Parson
authors.forename: Cordula
authors.initials: C
authors.lastname: Eichmann
authors.forename: Simone
authors.initials: S
authors.lastname: Millinger
authors.forename: Heidi
authors.initials: H
authors.lastname: Fiegl
authors.forename: Raimund
authors.initials: R
authors.lastname: Margreiter
authors.forename: Albert
authors.initials: A
authors.lastname: Amberger
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D003106:Colon / Q000378:metabolism; D018899:CpG Islands; D019175:DNA Methylation; D000077209:Decitabine; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D012333:RNA, Messenger; D000077209:Decitabine; D001374:Azacitidine