Improvement of Bacillus thuringiensis delta-endotoxins synthesis yields through acquisition of erythromycin resistance.
The acquisition of the erythromycin resistance by Bacillus thuringiensis kurstaki improved yields of delta-endotoxins in sporulating cells ranging from 134 to 215%. Resistance to erythromycin decreased the final spore count by at least 50%. Consequently, erythromycin resistance is an efficient tool for the improvement of bioinsecticides yields with a high ratio of delta-endotoxins to spores.
Tounsi
Slim
S
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, Tunisia P.O. Box K, 3038, Sfax, Tunisia.
Zouari
Nabil
N
Ghribi
Dhouha
D
J'mal
Anis
A
Jaoua
Samir
S
D001413:Bacillus thuringiensis / Q000502:physiology*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000502:physiology*; D004731:Endotoxins / Q000096:biosynthesis* / Q000235:genetics*; D004917:Erythromycin / Q000008:administration & dosage*; D024861:Genetic Enhancement / Q000379:methods; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D011498:Protein Precursors / Q000096:biosynthesis*
D000083722:Bacillus thuringiensis Toxins; C029103:Bacillus thuringiensis protoxin; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011498:Protein Precursors; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D004917:Erythromycin