title: Effective therapy of transected quadriceps muscle in rat: Gastric pentadecapeptide BPC 157.
abstract: We report complete transection of major muscle and the systemic peptide treatment that induces healing of quadriceps muscle promptly and then maintains the healing with functional restoration. Initially, stable gastric pentadecapeptide BPC 157 (GEPPPGKPADDAGLV, M.W. 1419, PL-10, PLD-116, PL 14736 Pliva, Croatia; in trials for inflammatory bowel disease; wound treatment; no toxicity reported; effective alone without carrier) also superiorly accelerates the healing of transected Achilles tendon. Regularly, quadriceps muscle completely transected transversely 1.0 cm proximal to patella presents a definitive defect that cannot be compensated in rat. BPC 157 (10 microg, 10 ng, 10 pg/kg) is given intraperitoneally, once daily; the first application 30 min posttransection, the final 24 h before sacrifice. It consistently improves muscle healing throughout the whole 72-day period. Improved are: (i) biomechanic (load of failure increased); (ii) function (walking recovery and extensor postural thrust/motor function index returned toward normal healthy values); (iii) microscopy/immunochemistry [i.e., mostly muscle fibers connect muscle segments; absent gap; significant desmin positivity for ongoing regeneration of muscle; larger myofibril diameters on both sides, distal and proximal (normal healthy rat-values reached)]; (iv) macroscopic presentation (stumps connected; subsequently, atrophy markedly attenuated; finally, presentation close to normal noninjured muscle, no postsurgery leg contracture). Thus, posttransection healing-consistently improved-may suggest this peptide therapeutic application in muscle disorders.
authors.affiliation: Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, P.O.B. 916, 10000 Zagreb, Croatia.
authors.forename: Mario
authors.initials: M
authors.lastname: Staresinic
authors.forename: Igor
authors.initials: I
authors.lastname: Petrovic
authors.forename: Tomislav
authors.initials: T
authors.lastname: Novinscak
authors.forename: Ivana
authors.initials: I
authors.lastname: Jukic
authors.forename: Damira
authors.initials: D
authors.lastname: Pevec
authors.forename: Slaven
authors.initials: S
authors.lastname: Suknaic
authors.forename: Neven
authors.initials: N
authors.lastname: Kokic
authors.forename: Lovorka
authors.initials: L
authors.lastname: Batelja
authors.forename: Luka
authors.initials: L
authors.lastname: Brcic
authors.forename: Alenka
authors.initials: A
authors.lastname: Boban-Blagaic
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Zoric
authors.forename: Domagoj
authors.initials: D
authors.lastname: Ivanovic
authors.forename: Marko
authors.initials: M
authors.lastname: Ajduk
authors.forename: Bozidar
authors.initials: B
authors.lastname: Sebecic
authors.forename: Leonardo
authors.initials: L
authors.lastname: Patrlj
authors.forename: Tomislav
authors.initials: T
authors.lastname: Sosa
authors.forename: Gojko
authors.initials: G
authors.lastname: Buljat
authors.forename: Tomislav
authors.initials: T
authors.lastname: Anic
authors.forename: Sven
authors.initials: S
authors.lastname: Seiwerth
authors.forename: Predrag
authors.initials: P
authors.lastname: Sikiric
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001696:Biomechanical Phenomena; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000293:injuries* / Q000503:physiopathology; D010446:Peptide Fragments / Q000627:therapeutic use*; D011506:Proteins / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013708:Tendon Injuries / Q000188:drug therapy* / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010446:Peptide Fragments; D011506:Proteins; C083833:BPC 157