title: Anatomical and molecular characterization of Lactarius aff. omphaliformis, Russula alnijorullensis and Cortinarius tucumanensis ectomycorrhizae on Alnus acuminata.
abstract: Ectomycorrhizae (ECM) of Lactarius aff. omphaliformis Romagn., Russula alnijorullensis (Sing.) Sing. and Cortinarius tucumanensis Mos. on Andean alder (Alnus acuminata Kunth) were characterized and identified. The identification of the fungal symbionts was achieved by morpho-anatomical observations of mycorrhizae and by comparison of ITS-RFLP patterns obtained from ECM and fruitbodies. L. aff omphaliformis ECM differed in some morphological details such as ramification and mantle type from ECM of the same species on A. glutinosa. L. aff omphaliformis ECM show an orange to ochre mantle containing latex cells, which stain with sulpho-vanillin, emanating hyphae without clamps. R. alnijorullensis ECM represent a typical Russula-type-ECM, light yellow to pinkish, the outer mantle being composed of triangular latex-filled cells staining with sulpho-vanillin, emanating hyphae without clamps. C. tucumanensis ECM exhibit a white (silvery) to yellowish brown mantle covered with soil particles, emanating hyphae with clamps.
authors.affiliation: Instituto Multidisciplinario de Biología Vegetal (CONICET), Córdoba, Argentina. abecerra@imbiv.unc.edu.ar
authors.forename: Alejandra
authors.initials: A
authors.lastname: Becerra
authors.forename: Ludwig
authors.initials: L
authors.lastname: Beenken
authors.forename: Karin
authors.initials: K
authors.lastname: Pritsch
authors.forename: Graciela
authors.initials: G
authors.lastname: Daniele
authors.forename: Michael
authors.initials: M
authors.lastname: Schloter
authors.forename: Reinhard
authors.initials: R
authors.lastname: Agerer
mesh_terms: D000363:Agaricales / Q000145:classification / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D029661:Alnus / Q000382:microbiology*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D025301:Hyphae / Q000166:cytology; D038821:Mycorrhizae / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D010784:Photomicrography; D010860:Pigments, Biological; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013194:Staining and Labeling; D013559:Symbiosis
chemical_list: D004271:DNA, Fungal; D010860:Pigments, Biological