title: [The expression of mutation p53 gene from circulating cancer cells of peripheral blood and the clinical significance in detecting the patients with colorectal cancer].
abstract: OBJECTIVE
  To study the clinical significance of detecting p53 gene mutation expression in colorectal cancer cells of peripheral blood.
  
  
  METHODS
  Flow cytometry (FCM) was used to detect p53 gene mutation expression in peripheral blood cancer cells of 128 patients with colorectal cancer. Experimental data were analyzed by SPSS (v.11.0) software.
  
  
  RESULTS
  The lymph node metastasis showed the significant difference statistically (P<0.01) between p53 positive and negative expression in the colorectal cancer patients. The mutation p53 expression associated with existing histological differentiation (r=0.8476, P<0.05). A lymph node metastasis difference was observed between left and right colorectal cancers of mutation p53 positive expression.
  
  
  CONCLUSIONS
  Detecting the mutation p53 expression in cancer cells of peripheral blood might be helpful to the early diagnosis of colorectal cancer.
authors.affiliation: Laboratory of Genetics, Jiangsu Institute for Cancer Research, Nanjing, Jiangsu, 210009 P.R.China.
authors.forename: Guo-jian
authors.initials: GJ
authors.lastname: Ma
authors.forename: Jian-nong
authors.initials: JN
authors.lastname: Zhou
authors.forename: Zong-li
authors.initials: ZL
authors.lastname: Shen
authors.forename: Sen-qing
authors.initials: SQ
authors.lastname: Chen
authors.forename: Yue-qing
authors.initials: YQ
authors.lastname: Zhu
authors.forename: Xiao-liu
authors.initials: XL
authors.lastname: Wu
authors.forename: Kai-xian
authors.initials: KX
authors.lastname: Xue
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53