title: Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
abstract: The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.
authors.affiliation: School of Chemistry, Centre for Biomolecular Sciences, University of St Andrews, Purdie Building, North Haugh, St Andrews, KY16 9ST, UK.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Cobb
authors.forename: Hai
authors.initials: H
authors.lastname: Deng
authors.forename: Andrew R
authors.initials: AR
authors.lastname: McEwan
authors.forename: James H
authors.initials: JH
authors.lastname: Naismith
authors.forename: David
authors.initials: D
authors.lastname: O'Hagan
authors.forename: David A
authors.initials: DA
authors.lastname: Robinson
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D002712:Chlorides / Q000737:chemistry; D018360:Crystallography, X-Ray; D003839:Deoxyadenosines / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry; D006860:Hydrogen Bonding; D008958:Models, Molecular; D015394:Molecular Structure; D010088:Oxidoreductases / Q000737:chemistry*; D013302:Streptomyces / Q000145:classification / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D002712:Chlorides; D003839:Deoxyadenosines; D010088:Oxidoreductases; C495766:fluorinase; C058118:2'-deoxyadenosine; D005459:Fluorides