Adhesion of Borrelia garinii to neuronal cells is mediated by the interaction of OspA with proteoglycans.
To study pathogenic mechanisms of Lyme meningoradiculitis, dorsal root ganglia (DRG) cells and two neuronal cell lines (B50, SH-SY5Y) were incubated with Borrelia garinii, the Borrelia species most frequently isolated from CSF of Lyme neuroborreliosis patients in Europe. We demonstrated that (I) OspA-positive B. garinii adhere to neuronal cells, (II) Borrelia adhesion can be blocked by a monoclonal antibody against OspA, (III) preincubation with proteoglycans interferes with the adhesion process and (IV) rOspA directly binds to the proteoglycans. This indicates that both OspA and the cell bound proteoglycans are involved in the attachment of B. garinii to neuronal cells.
Rupprecht
Tobias A
TA
Department of Neurology, Klinikum Grosshadern, Ludwig-Maximilians University, Marchioninistr. 15, D-81377 Munich, Germany.
Koedel
Uwe
U
Heimerl
Christiane
C
Fingerle
Volker
V
Paul
Robert
R
Wilske
Bettina
B
Pfister
Hans-Walter
HW
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001428:Bacterial Vaccines; D015748:Borrelia burgdorferi Group / Q000302:isolation & purification / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D005727:Ganglia, Spinal / Q000378:metabolism; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D020852:Lyme Neuroborreliosis / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats
D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D015815:Cell Adhesion Molecules; D008074:Lipoproteins; C072045:OspA protein; D011509:Proteoglycans