Misexpression screen for genes altering the olfactory map in Drosophila.
Despite the identification of a number of guidance molecules, a comprehensive picture has yet to emerge to explain the precise anatomy of the olfactory map. From a misexpression screen of 1,515 P{GS} lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of the Drosophila antennal lobes. These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling. We analyzed Akap200, which encodes a Protein Kinase A-binding protein. Overexpression of Akap200 resulted in fusion of the glomeruli, while its loss resulted in misshapen and ectopic glomeruli. The requirement of Akap200 validates our screen as an effective approach for recovering genes controlling glomerular map patterning. Our finding of diverse classes of genes reveals the complexity of the mechanisms that underlie olfactory map development.
Zhang
Dongsheng
D
Cell and Developmental Biology, University of Illinois at Urbana-Champaign, 601 South Goodwin Avenue, Urbana, IL 61801, USA.
Zhou
Weiguang
W
Yin
Chong
C
Chen
Weitao
W
Ozawa
Rie
R
Ang
Lay-Hong
LH
Anandan
Lavanya
L
Aigaki
Toshiro
T
Hing
Huey
H
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004330:Drosophila / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D017344:Genes, Insect; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008953:Models, Anatomic; D008957:Models, Genetic; D009833:Olfactory Pathways / Q000033:anatomy & histology / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000502:physiology*
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; C121679:Akap200 protein, Drosophila; D029721:Drosophila Proteins; D008565:Membrane Proteins