title: Whitefly (Bemisia tabaci) genome project: analysis of sequenced clones from egg, instar, and adult (viruliferous and non-viruliferous) cDNA libraries.
abstract: BACKGROUND
  The past three decades have witnessed a dramatic increase in interest in the whitefly Bemisia tabaci, owing to its nature as a taxonomically cryptic species, the damage it causes to a large number of herbaceous plants because of its specialized feeding in the phloem, and to its ability to serve as a vector of plant viruses. Among the most important plant viruses to be transmitted by B. tabaci are those in the genus Begomovirus (family, Geminiviridae). Surprisingly, little is known about the genome of this whitefly. The haploid genome size for male B. tabaci has been estimated to be approximately one billion bp by flow cytometry analysis, about five times the size of the fruitfly Drosophila melanogaster. The genes involved in whitefly development, in host range plasticity, and in begomovirus vector specificity and competency, are unknown.
  
  
  RESULTS
  To address this general shortage of genomic sequence information, we have constructed three cDNA libraries from non-viruliferous whiteflies (eggs, immature instars, and adults) and two from adult insects that fed on tomato plants infected by two geminiviruses: Tomato yellow leaf curl virus (TYLCV) and Tomato mottle virus (ToMoV). In total, the sequence of 18,976 clones was determined. After quality control, and removal of 5,542 clones of mitochondrial origin 9,110 sequences remained which included 3,843 singletons and 1,017 contigs. Comparisons with public databases indicated that the libraries contained genes involved in cellular and developmental processes. In addition, approximately 1,000 bases aligned with the genome of the B. tabaci endosymbiotic bacterium Candidatus Portiera aleyrodidarum, originating primarily from the egg and instar libraries. Apart from the mitochondrial sequences, the longest and most abundant sequence encodes vitellogenin, which originated from whitefly adult libraries, indicating that much of the gene expression in this insect is directed toward the production of eggs.
  
  
  CONCLUSIONS
  This is the first functional genomics project involving a hemipteran (Homopteran) insect from the subtropics/tropics. The B. tabaci sequence database now provides an important tool to initiate identification of whitefly genes involved in development, behaviour, and B. tabaci-mediated begomovirus transmission.
authors.affiliation: The Hebrew University Bioinformatics Unit, The Hebrew University of Jerusalem, Rehovot 76100, Israel. dena@agri.huji.ac.il
authors.forename: Dena
authors.initials: D
authors.lastname: Leshkowitz
authors.forename: Shirley
authors.initials: S
authors.lastname: Gazit
authors.forename: Eli
authors.initials: E
authors.lastname: Reuveni
authors.forename: Murad
authors.initials: M
authors.lastname: Ghanim
authors.forename: Henryk
authors.initials: H
authors.lastname: Czosnek
authors.forename: Cindy
authors.initials: C
authors.lastname: McKenzie
authors.forename: Robert L
authors.initials: RL
authors.lastname: Shatters
authors.forename: Judith K
authors.initials: JK
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020451:Contig Mapping; D030541:Databases, Genetic; D004330:Drosophila / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D017344:Genes, Insect / Q000235:genetics*; D049750:Genome, Insect / Q000235:genetics*; D006430:Hemiptera / Q000235:genetics*; D007814:Larva / Q000235:genetics*; D008969:Molecular Sequence Data; D010063:Ovum / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014819:Vitellogenins / Q000737:chemistry / Q000235:genetics
chemical_list: D014819:Vitellogenins