Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas.
Pacific oysters, Crassostrea gigas, are commonly reared in estuaries where they are exposed to anthropogenic pollution. Much research has been made on the toxicity of cadmium to aquatic organisms because the compound recurrently contaminates their environment. Our study examined the influence of cadmium on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in C. gigas at different stages of life. Adults and juveniles were exposed to two different concentrations of cadmium. The first concentration applied was equivalent to a peak value found in Marennes-Oléron bay (Charente-Maritime, France; 50 ngL(-1)) and the second was 10 times higher (500 ngL(-1)). Exposure to 50 ngL(-1) cadmium caused a significant decrease in the survival time of C. gigas, but exposure to 500 ngL(-1) surprisingly affected the survival time positively. Significant differences in aneuploidy level were observed between the cadmium treatments and the control in adults but not in juveniles or the offspring of the adult groups. The effects of cadmium on hemocyte parameters were analyzed by flow cytometry. Several hemocyte parameters increased significantly after 21 days of cadmium exposure and subsequently decreased. Phenoloxidase-like activity, evaluated by spectrophotometry, varied over the time of the experiment and increased after 66 days of contact with 500 ngL(-1) cadmium. Taken together, cadmium at environmentally relevant concentrations seems to have only moderate effects on aneuploidy and hemocyte parameters.
Bouilly
Karine
K
IFREMER, Laboratoire de Génétique et Pathologie, La Tremblade 17390, France.
Gagnaire
Béatrice
B
Bonnard
Marc
M
Thomas-Guyon
Hélène
H
Renault
Tristan
T
Miramand
Pierre
P
Lapègue
Sylvie
S
D000367:Age Factors; D000782:Aneuploidy; D000818:Animals; D049628:Body Size / Q000187:drug effects; D002104:Cadmium / Q000633:toxicity*; D049896:Crassostrea / Q000187:drug effects*; D004781:Environmental Exposure; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D048788:Growth and Development / Q000187:drug effects; D006434:Hemocytes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D014442:Monophenol Monooxygenase / Q000187:drug effects / Q000378:metabolism; D009152:Mutagenicity Tests / Q000662:veterinary; D016019:Survival Analysis; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D002104:Cadmium; D014442:Monophenol Monooxygenase