Mitochondrial cytochrome c reacts with nitric oxide via S-nitrosation.
The present study demonstrates that mitochondrial cytochrome c reacts with the thiol-reacting agent N-ethylmaleimide (NEM) to produce a one NEM-adducted cytochrome c. Mitochondrial cytochrome c also reacts with 5,5'-dithio-bis-(2-nitrobenzoic acid) and 1-chloro-2,4-dinitrobenzene in a manner prevented with NEM or iodoacetic acid (IAA). NEM-treated cytochrome c has lower reducibility and lower function to support mitochondrial oxygen consumption. These findings suggest that mitochondrial cytochrome c contains a reactive thiol that is involved in the functions of cytochrome c for mitochondria. Nitric oxide reacts with the cytochrome c thiol to generate S-nitroso (SNO)-cytochrome c in a manner prevented with NEM or IAA. SNO-cytochrome c has lower reducibility and function to support mitochondrial oxygen consumption, similar to NEM-treated cytochrome c.
Larson
Sarah K
SK
Vascular Surgery and Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, USA.
Dwyer
Donard
D
Lo
Herng-Hsiang
HH
Ghafourifar
Pedram
P
D000818:Animals; D045304:Cytochromes c / Q000378:metabolism*; D006736:Horses; D008928:Mitochondria / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D015538:Nitrosation; D013438:Sulfhydryl Compounds / Q000378:metabolism
D013438:Sulfhydryl Compounds; D009569:Nitric Oxide; D045304:Cytochromes c