[Effect of Shenkangwan on mesangial cell NO and TGF-beta1 excretion in rats with early diabetic nephropathy].
OBJECTIVE
To study the mechanism of Shenkangwan (SKW) in treating early diabetic nephropathy (DN).


METHODS
The effect of SKW on NO and transforming growth factor (TGF)-beta(1) production by the mesangial cells (MCs) of rats with early diabetic nephropathy was evaluated with serum pharmacological method.


RESULTS
Compared with normal serum, the SKW-containing serum dose- and time-dependently inhibited TGF-beta(1) excretion and increased NO production in the MCs of rats with early DN (P<0.05 and P<0.01, respectively).


CONCLUSIONS
The therapeutic effect of SKW on early DN may rely on the balance modulation of cytokine network by increasing NO production and decreasing TGF-beta(1) excretion to prevent cytokine-induced damage of the MCs.
Chen
Guo-bao
GB
Center of Integrated Traditional and Western Nephrology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Wei
Lian-bo
LB
Xiao
Wei
W
Long
Hai-bo
HB
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005920:Glomerular Mesangium / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*
D004365:Drugs, Chinese Herbal; D016212:Transforming Growth Factor beta; D009569:Nitric Oxide