DNA microarray analysis of anaerobic Methanosarcina barkeri reveals responses to heat shock and air exposure.
Methanosarcina barkeri is a methanogenic archaeon that can digest cellulose and other polysaccharides to produce methane. It can only grow under strictly anoxic conditions, but which can survive air exposure. To obtain further knowledge of cellular changes occurring in M. barkeri in response to air exposure and other environmental stresses, we constructed the first oligonucleotide microarray for M. barkeri and used it to investigate the global transcriptomic responses of M. barkeri to air exposure and heat shock at 45 degrees C for 1 h. The results showed that various house-keeping genes, such as genes involved in DNA replication recombination and repair, energy production and conversion, and protein turnover were regulated by environmental stimuli. In response to air exposure, up-regulation of a large number of transposase encoding genes was observed. However, no differential expression of genes encoding superoxide dismutase, catalase, nonspecific peroxidases or thioredoxin reductase was observed in response to air exposure, implying that no significant level of reactive oxygen species has been formed under air exposure. In response to heat shock, both Hsp70 (DnaK-DnaJ-GrpE chaperone system) the Hsp60 (GroEL) systems were up-regulated, suggesting that they may play an important role in protein biogenesis in M. barkeri during heat stress.
Zhang
Weiwen
W
Microbiology Department, Pacific Northwest National Laboratory, Richland, WA 99352, USA. Weiwen.Zhang@pnl.gov
Culley
David E
DE
Nie
Lei
L
Brockman
Fred J
FJ
D000388:Air; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics*; D017021:Methanosarcina barkeri / Q000235:genetics* / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis
D001426:Bacterial Proteins