[Inhibitory effect of dendritic cell vaccine on the growth of implanted tongue squamous cell carcinoma tumors in nude mice].
OBJECTIVE
To investigate the inhibitory effect of dendritic cells (DCs) on the growth of the implanted tongue squamous cell carcinoma (SCC) tumors in nude mice.


METHODS
Being induced from human peripheral blood monocytes (PBMCs) with rhGM-CSF, rhIL-4 and rhTNF-alpha, DCs were pulsed by Tca8113 cells lysates. Those DCs and T lymphocytes were co-cultured to induce specific cytotoxic T lymphocytes (CTLs). Immunotherapy was then performed after those DCs and CTLs were implanted into the tumor-bearing nude mice.


RESULTS
DCs were induced from PBMCs with multiple cytokines. Compared with the control groups, the growth of tumors was significantly inhibited in the group that had been implanted with DCs and CTLs, and tumor doubling time also increased markedly (P < 0.05).


CONCLUSIONS
DCs function normally after being induced from human PBMCs with multiple cytokines; DCs pulsed with tumor cell lysates and CTLs co-cultured with those DCs have a significant anti-tumor immune activity in nude mice.
Han
Wei
W
Department of Oral and Maxillofacial Surgery, The Stomatological Hospital Affiliated to Medical college of Nanjing University, Nanjing 210008, China.
Hu
Qin-gang
QG
Wang
Zhi-yong
ZY
D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000628:therapy*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D007167:Immunotherapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000628:therapy; D014062:Tongue Neoplasms / Q000628:therapy*
D019496:Cancer Vaccines