title: Second stage production of iturin A by induced germination of Bacillus subtilis RB14.
abstract: Bacillus subtilis RB14, a dual producer of lipopeptide antibiotics iturin A and surfactin undergoes sporulation in the submerged fermentation and the production of these secondary metabolites becomes halted. In this study, production of lipopeptide antibiotics was investigated by induced germination of the spores by heat-activation and nutrient supplementation. The induced spores became metabolically active vegetative state and produced lipopeptide antibiotic iturin A that added up the total production at the end of the fermentation. However, additional production of surfactin was not observed. This second time iturin A production by the germinated cells from the spores was defined as second stage production.
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan. rahman@res.titech.ac.jp
authors.forename: Mohammad Shahedur
authors.initials: MS
authors.lastname: Rahman
authors.forename: Takashi
authors.initials: T
authors.lastname: Ano
authors.forename: Makoto
authors.initials: M
authors.lastname: Shoda
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D005285:Fermentation / Q000502:physiology; D007218:Industrial Microbiology / Q000379:methods; D055666:Lipopeptides; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D013171:Spores, Bacterial / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D055666:Lipopeptides; D010456:Peptides, Cyclic; C009365:surfactin peptide; C013579:iturin A