[The action of dexamethasone on trans epithelial electrical resistance and the expression of aquaporin-1 in immortalized trabecular cells].
OBJECTIVE
To study the effect of dexamethasone on trans epithelial electrical resistance (TEER) and the expression of AQP-1 in immortalized trabecular cells.


METHODS
Immortalized human trabecular cells were cultured on PET membrane in 24 wells plate. The cells were treated with Dexamethasone in concentration of 10(-7) mol/L. After the cultured cell became confluence, TEER of the cells was detected to evaluate the resistance of outflow pathway at different time points. Western blot was used to analyze the expression of AQP-1 in the trabecular cells.


RESULTS
The TEER and the expression of AQP-1 were much higher in the cells treated by Dexamethasone in comparison to control without dexamethasone exposure.


CONCLUSIONS
The TEER and expression of AQP-1 in immortalized trabecular cells can be enhanced by Dexamethasone. AQP-1 upregulation induced by Glucocorticoid may relate with the increased resistance of outflow pathway in glaucoma.
Zhuo
Ye-hong
YH
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Ge
Jian
J
Huang
Ya-lin
YL
Wei
Yan-taoi
YT
Ling
Yun-lan
YL
Lin
Ming-ka
MK
D051398:Aquaporin 1 / Q000096:biosynthesis*; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D017097:Electric Impedance; D006801:Humans; D014129:Trabecular Meshwork / Q000187:drug effects* / Q000503:physiopathology
D051398:Aquaporin 1; D003907:Dexamethasone