The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii.
ISAba1 was found in all widespread clones of Acinetobacter baumannii in the United Kingdom. All isolates studied had a blaOXA-51-like carbapenemase gene; some also had blaOXA-23-like and/or blaOXA-58-like. Among isolates with blaOXA-51-like as sole carbapenemase gene, only those with ISAba1 adjacent to blaOXA-51-like were carbapenem resistant. Minor differences in blaOXA-51-like sequence were observed in resistant and susceptible isolates. Isolates with blaOXA-23-like in addition were consistently resistant to carbapenems; in all of these ISAba1 lay upstream of blaOXA-23-like, but was not associated with blaOXA-51-like. These results suggest that ISAba1 is providing the promoter for blaOXA-51-like and, probably, for blaOXA-23-like.
Turton
Jane F
JF
Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, London, UK. jane.turton@hpa.org.uk
Ward
M Elaina
ME
Woodford
Neil
N
Kaufmann
Mary E
ME
Pike
Rachel
R
Livermore
David M
DM
Pitt
Tyrone L
TL
D040981:Acinetobacter baumannii / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004251:DNA Transposable Elements / Q000502:physiology*; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases; C063912:carbapenemase