[Development of a novel in-vitro culture device simulating distraction osteogenesis and expression of osteoblast cytoskeleton].
OBJECTIVE
To establish a novel in-vitro culture device simulating distraction osteogenesis, and to understand the the changes of cytoskeleton of osteoblasts under different magnitudes of mechanical distraction.


METHODS
A novel in-vitro culture device simulating distraction osteogenesis was developed with titanium and silicon membrane. The expressions of F-actin cytoskeleton subjected to different magnitudes of distraction were observed in situ under a confocal laser scanning microscope, and protein levels of actin and tubulin alpha were analyzed by electrophoresis of Western blot.


RESULTS
The mode of culture device simulating distraction osteogenesis was similar to that of distraction osteogenesis used in clinic. After 24 h mechanical distraction with physiological magnitude, F-actin of osteoblasts was organized and paralleled to the direction of distraction forces. After 24 h mechanical distraction with hyperphysiological magnitude, however, F-actin of osteoblasts became reorganized and disrupted, and protein levels of actin and tubulin alpha decreased.


CONCLUSIONS
The novel in vitro culture device simulating distraction osteogenesis is suitable for the study of distraction osteogenesis in vitro. Physiological mechanical distraction causes organization and rearrangement of actin cytoskeleton. However, hyperphysiological mechanical distraction results in reorganization and disruption of actin cytoskeleton.
Wang
Zhi-ying
ZY
Department of Stomatology, The Second Affiliated Hospital, Jinzhou Medical College, Jinzhou Liaoning 121001, China.
Wang
Xing
X
Xiao
Jun-jun
JJ
Dong
Xiao-min
XM
D000199:Actins / Q000096:biosynthesis*; D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000502:physiology*; D008954:Models, Biological; D010006:Osteoblasts / Q000166:cytology*; D019857:Osteogenesis, Distraction; D051381:Rats; D014404:Tubulin / Q000096:biosynthesis
D000199:Actins; D014404:Tubulin