title: Protocadherin-PC promotes androgen-independent prostate cancer cell growth.
abstract: BACKGROUND
  Protocadherin-PC (PCDH-PC) expression is upregulated in apoptosis-resistant sublines of the LNCaP human prostate cancer (CaP) cell line. Here, we assess the role of PCDH-PC in CaP cells and its mRNA expression in human prostate tissues.
  
  
  METHODS
  LNCaP cells transfected with PCDH-PC were tested for their ability to grow in vitro and in vivo in androgen-deprived conditions. PCDH-PC mRNA expression was evaluated by semi-quantitative RT-PCR and by in situ hybridization.
  
  
  RESULTS
  PCDH-PC expression induced Wnt signaling in CaP cells and permitted androgen-independent growth of hormone-sensitive CaP cells. Expression of PCDH-PC-homologous transcripts was low and restricted to some epithelial cells in normal tissue and to CaP cells in tumors. However, hormone-resistant CaP cells expressed significantly higher levels of PCDH-PC-related mRNA.
  
  
  CONCLUSIONS
  Our findings suggest a novel mechanism for the progression of CaP involving expression of PCDH-PC. This novel protocadherin induces Wnt signaling, promotes malignant behavior and hormone-resistance of CaP cells.
authors.affiliation: Department of Urology and Pathology of CHU Henri Mondor, INSERM E 03-37, Université Paris XII, Centre de Recherches Chirurgicales, Assistance Publique des Hôpitaux de Paris, Créteil, France.
authors.forename: Stephane
authors.initials: S
authors.lastname: Terry
authors.forename: Luis
authors.initials: L
authors.lastname: Queires
authors.forename: Sixtina
authors.initials: S
authors.lastname: Gil-Diez-de-Medina
authors.forename: Min-Wei
authors.initials: MW
authors.lastname: Chen
authors.forename: Alexandre
authors.initials: A
authors.lastname: de la Taille
authors.forename: Yves
authors.initials: Y
authors.lastname: Allory
authors.forename: Phuong-Lan
authors.initials: PL
authors.lastname: Tran
authors.forename: Claude C
authors.initials: CC
authors.lastname: Abbou
authors.forename: Ralph
authors.initials: R
authors.lastname: Buttyan
authors.forename: Francis
authors.initials: F
authors.lastname: Vacherot
mesh_terms: D000728:Androgens / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015820:Cadherins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009376:Neoplasms, Hormone-Dependent / Q000737:chemistry / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D011467:Prostate / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D000091363:Protocadherins; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D051820:TCF Transcription Factors / Q000502:physiology; D051153:Wnt Proteins / Q000235:genetics / Q000502:physiology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000728:Androgens; D015820:Cadherins; C468654:PCDH11Y protein, human; D000091363:Protocadherins; D012333:RNA, Messenger; D051820:TCF Transcription Factors; D051153:Wnt Proteins