Computational hyperspectral interferometry for studies of brain function: proof of concept.
Hyperspectral interferometric microscopy uses a unique combination of optics and algorithm design to extract information. Local brain activity rapidly changes local blood flow and red blood cell concentration (absorption) and oxygenation (color). We demonstrate that brain activity evoked during whisker stimulation can be detected with hyperspectral interferometric microscopy to identify the active whisker-barrel cortex in the rat brain. Information about constituent components is extracted across the entire spectral band. Algorithms can be flexibly optimized to discover, detect, quantify, and visualize a wide range of significant biological events, including changes relevant to the diagnosis and treatment of disease.
Fox
Douglas J
DJ
Department of Neurological Surgery, School of Medicine, Washington University, St Louis, Missouri 63110, USA.
Velde
Hana Tysver
HT
Preza
Chrysanthe
C
O'Sullivan
Joseph A
JA
Smith
William H
WH
Woolsey
Thomas A
TA
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000502:physiology*; D001931:Brain Mapping / Q000295:instrumentation* / Q000379:methods; D005073:Evoked Potentials, Somatosensory / Q000502:physiology*; D005240:Feasibility Studies; D005260:Female; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods