title: Evaluation of a Bacillus stearothermophilus tube test as a screening tool for anticoccidial residues in poultry.
abstract: A Bacillus stearothermophilus var. calidolactis C953 tube test was evaluated for its ability in detecting the residue of selected anticoccidial drugs in poultry, specially sulfamethazine, furazolidone, and amprolium. Various concentrations of each drug were injected into chicken liver and kidney tissues and these tissues were tested to determine the drug detection limits for each drug. The detection limit was defined as the drug concentration at which 95 % of the test results were interpreted as positive. The limits of detection in liver tissue were 0.35 microgram/ml for furazolidone, 0.70 microgram/ml for sulfamethazine and 7.80 microgram/ml for amprolium. In kidney tissues, they were 0.30 microgram/ml for furazolidone, 0.54 microgram/ml for sulfamethazine, and 7.6 microgram/ml for amprolium. It was concluded that this tube test could be used to screen for the residue of these three drugs in poultry.
authors.affiliation: Guidford Institute, Microbiology Laboratory, Egerton University Njoro, Njoro 20107, Kenya. ashitandi@lycos.com
authors.forename: Anakalo
authors.initials: A
authors.lastname: Shitandi
authors.forename: Aila
authors.initials: A
authors.lastname: Oketch
authors.forename: Symon
authors.initials: S
authors.lastname: Mahungu
mesh_terms: D000670:Amprolium / Q000032:analysis; D000818:Animals; D003049:Coccidiostats / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D005664:Furazolidone / Q000032:analysis; D001411:Geobacillus stearothermophilus / Q000187:drug effects*; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D011200:Poultry; D013418:Sulfamethazine / Q000032:analysis
chemical_list: D003049:Coccidiostats; D013418:Sulfamethazine; D005664:Furazolidone; D000670:Amprolium