title: Founder effect for the Ala431Glu mutation of the presenilin 1 gene causing early-onset Alzheimer's disease in Mexican families.
abstract: The etiology of Alzheimer's disease (AD) is complex. To date, molecular genetic studies in several families affected with AD have identified three genes associated with highly penetrant early-onset AD: Presenilin 1 (PSEN1), Presenilin 2 (PSEN2) and beta-amyloid precursor protein (APP); and one gene (apolipoprotein E) associated with late-onset AD. Molecular analysis of the PSEN1 gene was performed by direct sequencing of genomic DNA. The possible founder effect was investigated analyzing two highly polymorphic microsatellite markers flanking the PSEN1 gene. Twelve unrelated Mexican families with early-onset AD were analyzed. The Ala431Glu mutation in exon 12 of PSEN1 was found in nine (75%) of these families, which segregated showing autosomal dominant inheritance. Because all families bearing the mutation are from the State of Jalisco (located in Western Mexico), a founder effect was hypothesized. Microsatellite haplotype analysis suggested a common ancestor in these nine kindreds. In conclusion, the Ala431Glu mutation is a prevalent cause of early-onset familial Alzheimer's disease in families from the State of Jalisco, Mexico. Genetic evidence supports that it is a founder mutation descending from a single common ancestor. These findings have important implications for prompt diagnosis and genetic counseling for Mexican patients with familial AD from Jalisco.
authors.affiliation: Departamento de Neurogenética y Biología Molecular, lnstituto Nacional de Neurología y Neurocirugía Manuel Velasco Suarez, México D.F., México.
authors.forename: Petra
authors.initials: P
authors.lastname: Yescas
authors.forename: Adriana
authors.initials: A
authors.lastname: Huertas-Vazquez
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Villarreal-Molina
authors.forename: Astrid
authors.initials: A
authors.lastname: Rasmussen
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Tusié-Luna
authors.forename: Marisol
authors.initials: M
authors.lastname: López
authors.forename: Samuel
authors.initials: S
authors.lastname: Canizales-Quinteros
authors.forename: María Elisa
authors.initials: ME
authors.lastname: Alonso
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000209:etiology / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D005260:Female; D018703:Founder Effect; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D020125:Mutation, Missense; D011110:Polymorphism, Genetic; D053764:Presenilin-1 / Q000235:genetics*
chemical_list: D001057:Apolipoproteins E; D053764:Presenilin-1