title: Effects of maternal uninephrectomy on the development of fetal rat kidney: apoptosis and the expression of oncogenes.
abstract: The present study was designed to explore whether maternal uninephrectomy affects development of the collecting ducts in fetal kidney. Localization of DNA fragmented cells in the kidney of fetal rats from uninephrectomized mothers were examined by the terminal deoxynucleotidyl transferase (TdT)-mediated d-UTP-biotin nick end labeling (TUNEL) method. Immunohistochemistry was used to examine the localizations of bcl-2 gene products. The gene expressions for bcl-2, p53, and WT1 mRNAs were examined by using the semi-quantitative reverse transcript-polymerase chain reaction. TUNEL positive cells were more numerous in the medullary collecting ducts of the fetuses from uninephrectomized mothers than in those of the fetuses from sham-operated ones. The expressions of bcl-2, p53, and WT1 mRNAs were lower in the fetuses from uninephrectomized mothers than in the fetuses from sham-operated ones. Cells in the medullary collecting ducts showed positive reactions to anti-bcl-2 gene products antibody with the reactions being weaker in the fetuses from uninephrectomized mothers. These results showed that maternal uninephrectomy accelerated the development of fetal rat kidney and it was associated with the lowered the expression of bcl-2 in fetal rat kidney.
authors.affiliation: Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, Sakai, Japan. okada@vet.osakafu-u.ac.jp
authors.forename: Toshiya
authors.initials: T
authors.lastname: Okada
authors.forename: Koji
authors.initials: K
authors.lastname: Mitsuoka
authors.forename: Masaki
authors.initials: M
authors.lastname: Mino
authors.forename: Masafumi
authors.initials: M
authors.lastname: Mukamoto
authors.forename: Jun
authors.initials: J
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Morioka
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Morikawa
mesh_terms: D000818:Animals; D017209:Apoptosis; D005260:Female; D020287:In Situ Nick-End Labeling; D007668:Kidney / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D009035:Mothers; D009392:Nephrectomy; D011247:Pregnancy; D011270:Pregnancy, Animal; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D025721:WT1 Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D025721:WT1 Proteins