Polypeptide GalNAc-transferase T3 and familial tumoral calcinosis. Secretion of fibroblast growth factor 23 requires O-glycosylation.
Mutations in the gene encoding the glycosyltransferase polypeptide GalNAc-T3, which is involved in initiation of O-glycosylation, were recently identified as a cause of the rare autosomal recessive metabolic disorder familial tumoral calcinosis (OMIM 211900). Familial tumoral calcinosis is associated with hyperphosphatemia and massive ectopic calcifications. Here, we demonstrate that the secretion of the phosphaturic factor fibroblast growth factor 23 (FGF23) requires O-glycosylation, and that GalNAc-T3 selectively directs O-glycosylation in a subtilisin-like proprotein convertase recognition sequence motif, which blocks processing of FGF23. The study suggests a novel posttranslational regulatory model of FGF23 involving competing O-glycosylation and protease processing to produce intact FGF23.
Kato
Kentaro
K
Department of Medical Biochemistry and Genetics, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark.
Jeanneau
Charlotte
C
Tarp
Mads Agervig
MA
Benet-Pagès
Anna
A
Lorenz-Depiereux
Bettina
B
Bennett
Eric Paul
EP
Mandel
Ulla
U
Strom
Tim M
TM
Clausen
Henrik
H
D020816:Amino Acid Motifs; D000818:Animals; D001667:Binding, Competitive; D016466:CHO Cells; D002114:Calcinosis / Q000235:genetics* / Q000378:metabolism; D006224:Cricetinae; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D008659:Metabolic Diseases / Q000235:genetics; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D043484:Proprotein Convertases / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D013379:Substrate Specificity; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors; D017350:N-Acetylgalactosaminyltransferases; D009363:Neoplasm Proteins; D043484:Proprotein Convertases; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C000717427:FGF23 protein, human