The correlation dimension identifies B-cell immunoglobulin light chain restriction in peripheral blood flow cytometry data.
The correlation dimension (D2) is a mathematical tool that quantifies dimensional properties of fractals. We set out to determine whether immunoglobulin light chain restriction (LCR) in B-cell lymphoproliferative disorders (BCLPDs) could be identified using the D2. The D2 was calculated from flow cytometry data files (FCS 2.0) from a training set of peripheral blood specimens from 22 patients with and 23 patients without BCLPD. A cutoff value derived from the training set was applied to a validation set of 69 patients with and 64 patients without BCLPD. In the training set, all BCLPDs had a D2 of less than 0.72 (CD19-gated kappa-lambda data). When this cutoff was applied to the validation set, the D2 had sensitivity and specificity values of 88% and 98%, respectively. The D2 can identify LCR in peripheral blood flow cytometry data. It offers operator-independent data analysis and may be useful in objectively quantifying differences in data distribution.
Wongchaowart
Nicholas T
NT
Department of Clinical Pathology, Cleveland Clinic, Foundation, Cleveland, OH 44195, USA.
Rinehart
Daniel G
DG
Hsi
Eric D
ED
D018941:Antigens, CD19 / Q000378:metabolism; D001402:B-Lymphocytes; D005434:Flow Cytometry; D017709:Fractals; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000097:blood*; D008232:Lymphoproliferative Disorders / Q000097:blood*; D012680:Sensitivity and Specificity
D018941:Antigens, CD19; D007147:Immunoglobulin Light Chains