A translational enhancer element on the 3'-proximal end of the Panicum mosaic virus genome.
Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3'-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5'-proximal sgRNA genes in vitro.
Batten
Jeffrey S
JS
Department of Plant Pathology and Microbiology, Texas A&M University College Station, 77843-2132, USA.
Desvoyes
Benedicte
B
Yamamura
Yoshimi
Y
Scholthof
Karen-Beth G
KB
D020413:3' Untranslated Regions / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis / Q000235:genetics*; D038621:Regulatory Sequences, Ribonucleic Acid / Q000235:genetics*; D019183:Tombusviridae / Q000737:chemistry / Q000235:genetics*
D020413:3' Untranslated Regions; D038621:Regulatory Sequences, Ribonucleic Acid