title: Enterovirus 71 infection induces Fas ligand expression and apoptosis of Jurkat cells.
abstract: T-cell depletion is found in enterovirus 71 (EV71)-infected patients with pulmonary edema. However, the mechanism that causes T-cell depletion is unclear. To address this question, the effects of EV71 infection on the cell viability of human Jurkat T cells were studied. Viable viruses were recovered from both the culture supernatant and the cell lysate of Jurkat cells after EV71 infection. Results from reverse-transcription polymerase chain reaction (RT-PCR) and immunofluorescence assay confirmed further the presence of EV71 negative-strand RNA and antigen, respectively, in EV71-infected Jurkat cells. The viability of the Jurkat cells decreased after 48 hr of EV71 infection. Both terminal transferase end labeling (TUNEL) and DNA fragmentation assays demonstrated that the apoptosis of EV71-infected Jurkat cells had increased. In addition, the expression of Fas ligand (FasL) in EV71-infected Jurkat cells increased at both mRNA and surface expression levels. Taken together, these results confirmed that EV71 infected T cells and induced FasL expression, which may contribute to T-cell apoptosis during EV71 infection.
authors.affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Lien-Cheng
authors.initials: LC
authors.lastname: Chen
authors.forename: Huey-Wen
authors.initials: HW
authors.lastname: Shyu
authors.forename: Shun-Hua
authors.initials: SH
authors.lastname: Chen
authors.forename: Huan-Yao
authors.initials: HY
authors.lastname: Lei
authors.forename: Chun-Keung
authors.initials: CK
authors.lastname: Yu
authors.forename: Trai-Ming
authors.initials: TM
authors.lastname: Yeh
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002522:Chlorocebus aethiops; D004770:Enterovirus / Q000502:physiology*; D053222:Fas Ligand Protein; D005786:Gene Expression Regulation; D006801:Humans; D019169:Jurkat Cells; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D048069:Tumor Necrosis Factors / Q000235:genetics / Q000378:metabolism*; D014709:Vero Cells
chemical_list: C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D048069:Tumor Necrosis Factors