title: Expression of Tsga10 sperm tail protein in embryogenesis and neural development: from cilium to cell division.
abstract: Tsga10 has been localised in sperm tail as a fibrous sheath protein. In this study, we showed its expression during developmental stages of mouse embryo, in adult mice brain, and in some malignancies. RT-PCR and immunohistochemistry study show that Tsga10 expression starts in 4.5-7.5 dpc mouse embryos and continues throughout embryogenesis. Then we showed that the Tsga10 is expressed in adult brain and in the cells with neural crest origin, olfactory epithelium, and human germ cell tumour. It is expressed with two transcripts in sperm and whole embryos but just with the long transcript in brain embryo as a result of its exon 16 splicing. Our finding of the Tsga10 perinuclear localisation and its expression pattern suggests that it may be involved in active cell division, differentiation, and migrating cells. The results of the experiments in this project hypothesize the presence of Tsga10 protein wherever there is a conserved ciliary structure.
authors.affiliation: The Galton Laboratory, Department of Biology, University College London, London NW1 2HE, UK. bbehnam@umich.edu
authors.forename: Babak
authors.initials: B
authors.lastname: Behnam
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Modarressi
authors.forename: Valerio
authors.initials: V
authors.lastname: Conti
authors.forename: Kay E
authors.initials: KE
authors.lastname: Taylor
authors.forename: Aldamaria
authors.initials: A
authors.lastname: Puliti
authors.forename: Jonathan
authors.initials: J
authors.lastname: Wolfe
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D002923:Cilia / Q000378:metabolism; D003598:Cytoskeletal Proteins; D004622:Embryo, Mammalian / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D047108:Embryonic Development; D008297:Male; D051379:Mice; D009369:Neoplasms / Q000737:chemistry / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D029589:Seminal Plasma Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D013082:Sperm Tail / Q000737:chemistry / Q000378:metabolism; D013737:Testis / Q000737:chemistry / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D029589:Seminal Plasma Proteins; C493239:Tsga10 protein, mouse