Radioprotective effects of hesperidin against gamma irradiation in mouse bone marrow cells.
The radioprotective effects of hesperidin (HES), a flavonone glucoside, were investigated by using the micronucleus test for anticlastogenic and cell proliferation activity. A single intraperitoneal (ip) administration of hesperidin at doses of 10 mg kg(-1), 20 mg kg(-1), 40 mg kg(-1), 80 mg kg(-1) and 160 mg kg(-1) 45 min prior to gamma irradiation (2 Gy) reduced the frequencies of micronuleated polychromatic erythrocytes (MnPCEs). All five doses of HES significantly reduced the frequencies of MnPCEs and increased PCE/PCE+NCE ratio in mice bone marrow compared with non-drug-treated irradiated control (p<0.0001). There was a drug dose-response effect of HES in reducing MnPCE and increasing the PCE/PCE+NCE ratio in bone marrow cells. The maximum reduction in MnPCE was observed in mice treated with HES at a dose of 80 mg kg(-1). The total MnPCE values were 2.85 fold less in the 80 mg kg(-1) HES group after being exposed to 2 Gy of gamma-rays than those in the respective irradiated control. Our study demonstrates that hesperidin has powerful protective effects on the radiation-induced DNA damage and on the decline in cell proliferation in mouse bone marrow.
Hosseinimehr
S J
SJ
Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandarn University of Medical Sciences, Sari, Iran.
Nemati
A
A
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000528:radiation effects*; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002957:Citrus; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004912:Erythrocytes / Q000528:radiation effects / Q000648:ultrastructure; D005720:Gamma Rays / Q000009:adverse effects*; D006569:Hesperidin / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D010936:Plant Extracts / Q000627:therapeutic use; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D014916:Whole-Body Irradiation / Q000009:adverse effects*
D010936:Plant Extracts; D011837:Radiation-Protective Agents; D006569:Hesperidin