title: [Isolation and identification of Japanese encephalitis virus in Liaoning Province].
abstract: BACKGROUND
  To study arboviruses carried by mosquitoes in Liaoning Province in 2002.
  
  
  METHODS
  Totally 4927 mosquitoes were collected from Liaoning Province in July 2002. Virus strains were isolated by inoculating BHK-21, C6/36 and Vero cells. The newly isolated strains were identified by serological (ELISA and IFA) and molecular methods (Real-Time PCR and RT-PCR).
  
  
  RESULTS
  Two strains were isolated from mosquitoes causing cytopathogenic effect (CPE) on cells and were fatal to suckling mice. Serological tests showed that both were positive for the antibody to JEV. The PrM and E gene were cloned and sequenced. The phylogenetic analysis showed that the new isolates belonged to genotype I, JEV. Sequence analysis showed that the homology of nucleotide sequences and amino acid (AA) sequences between the two strains was 100%. Compared with the nucleotide sequences between the two strains and the standard JEV vaccine strain SA14-14-2, the difference was up to 4.11%, and the difference of AA was 0.6%.
  
  
  CONCLUSIONS
  Two strains of JEV were isolated and identified in Liaoning province, both belonged to genotype I JEV.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Jun-wen
authors.initials: JW
authors.lastname: Wang
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Huan-yu
authors.initials: HY
authors.lastname: Wang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Mao
authors.forename: Wei-bin
authors.initials: WB
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Sun
authors.forename: Zeng-lin
authors.initials: ZL
authors.lastname: Cai
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Wu
authors.forename: Xi-fang
authors.initials: XF
authors.lastname: Zhao
authors.forename: Rui-hong
authors.initials: RH
authors.lastname: Han
authors.forename: Ya
authors.initials: Y
authors.lastname: Jing
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000818:Animals; D002460:Cell Line; D002681:China; D002522:Chlorocebus aethiops; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C074932:prM protein, Flavivirus