Analysis of close stable homolog juxtaposition during meiosis in mutants of Saccharomyces cerevisiae.
A unique aspect of meiosis is the segregation of homologous chromosomes at the meiosis I division. The pairing of homologous chromosomes is a critical aspect of meiotic prophase I that aids proper disjunction at anaphase I. We have used a site-specific recombination assay in Saccharomyces cerevisiae to examine allelic interaction levels during meiosis in a series of mutants defective in recombination, chromatin structure, or intracellular movement. Red1, a component of the chromosome axis, and Mnd1, a chromosome-binding protein that facilitates interhomolog interaction, are critical for achieving high levels of allelic interaction. Homologous recombination factors (Sae2, Rdh54, Rad54, Rad55, Rad51, Sgs1) aid in varying degrees in promoting allelic interactions, while the Srs2 helicase appears to play no appreciable role. Ris1 (a SWI2/SNF2 related protein) and Dot1 (a histone methyltransferase) appear to play minor roles. Surprisingly, factors involved in microtubule-mediated intracellular movement (Tub3, Dhc1, and Mlp2) appear to play no appreciable role in homolog juxtaposition, unlike their counterparts in fission yeast. Taken together, these results support the notion that meiotic recombination plays a major role in the high levels of homolog interaction observed during budding yeast meiosis.
Lui
Doris Y
DY
Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA.
Peoples-Holst
Tamara L
TL
Mell
Joshua Chang
JC
Wu
Hsin-Yen
HY
Dean
Eric W
EW
Burgess
Sean M
SM
D000483:Alleles; D002843:Chromatin; D019457:Chromosome Breakage; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D004398:Dyneins / Q000235:genetics / Q000378:metabolism; D049469:Meiotic Prophase I; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D016601:RNA-Binding Proteins; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D004718:Saccharomycetales / Q000166:cytology / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D002843:Chromatin; C080988:MLP1 protein, S cerevisiae; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C475499:Tub3 protein, S cerevisiae; D004265:DNA Helicases; C505433:Dhc1 protein, S pombe; D004398:Dyneins