In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter.
We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.
Lee
Boo-Ja
BJ
School of Life Sciences and Biotechnology, Korea University, 1, 5-ga, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
Park
Chang-Jin
CJ
Kim
Sung-Kyu
SK
Kim
Ki-Jeong
KJ
Paek
Kyung-Hee
KH
D000595:Amino Acid Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000032:analysis / Q000378:metabolism*; D002212:Capsicum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D051790:G-Box Binding Factors / Q000032:analysis / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D014018:Tissue Distribution; D014026:Nicotiana / Q000235:genetics
D050976:Basic-Leucine Zipper Transcription Factors; D051790:G-Box Binding Factors; D009943:Organophosphorus Compounds; D010940:Plant Proteins; D012333:RNA, Messenger; C053376:pathogenesis-related proteins, plant; D020156:Salicylic Acid; C005073:ethephon