title: A testis-specific and testis developmentally regulated tumor protein D52 (TPD52)-like protein TPD52L3/hD55 interacts with TPD52 family proteins.
abstract: Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast cancer. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. To date, three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. Herein, we identified a novel TPD52-like sequence (TPD52L3, or hD55) in human testis using cDNA microarray. Sequence analysis of the deduced protein suggests that hD55 contains a coiled-coil motif and is highly conserved compared with other TPD52-like sequences. Yeast two-hybrid and GST pull-down assays revealed that hD55 interacts with hD52, hD53, hD54, and itself. cDNA microarray detection found that hD55 was expressed at 5.6-fold higher levels in adult testis than in fetal testis. Additionally, the expression profile shows that hD55 is testis-specific, indicating a potential role for hD55 in testis development and spermatogenesis.
authors.affiliation: Lab of Reproductive Medicine, Department of Cell Biology and Medical Genetics, Nanjing Medical University, Han Zhong Road 140, Nanjing, Jiangsu Province, China.
authors.forename: Qinhong
authors.initials: Q
authors.lastname: Cao
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jiahao
authors.initials: J
authors.lastname: Sha
authors.forename: Shui
authors.initials: S
authors.lastname: Wang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Li
mesh_terms: D000375:Aging / Q000502:physiology*; D000595:Amino Acid Sequence; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D011485:Protein Binding; D025941:Protein Interaction Mapping; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000502:physiology*; D014018:Tissue Distribution
chemical_list: D009363:Neoplasm Proteins; C114751:TPD52 protein, human