title: A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells.
abstract: In the subventricular zone of the adult mammalian forebrain, neural stem cells (NSCs) reside and proliferate to generate young neurons. We screened factors that promoted the proliferation of NSCs in vitro by a recently developed proteomics technique, the ProteinChip system. In this screen, we identified a soluble carbohydrate-binding protein, Galectin-1, as a candidate. We show herein that Galectin-1 is expressed in a subset of slowly dividing subventricular zone astrocytes, which includes the NSCs. Based on results from intraventricular infusion experiments and phenotypic analyses of knockout mice, we demonstrate that Galectin-1 is an endogenous factor that promotes the proliferation of NSCs in the adult brain.
authors.affiliation: Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Shingo
authors.forename: Takuya
authors.initials: T
authors.lastname: Shimazaki
authors.forename: Hirotaka James
authors.initials: HJ
authors.lastname: Okano
authors.forename: Mieko
authors.initials: M
authors.lastname: Shiwa
authors.forename: Satoru
authors.initials: S
authors.lastname: Ishibashi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Oguro
authors.forename: Mikiko
authors.initials: M
authors.lastname: Ninomiya
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Kadoya
authors.forename: Hidenori
authors.initials: H
authors.lastname: Horie
authors.forename: Akira
authors.initials: A
authors.lastname: Shibuya
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Mizusawa
authors.forename: Françoise
authors.initials: F
authors.lastname: Poirier
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Nakauchi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Sawamoto
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology; D002241:Carbohydrates; D049109:Cell Proliferation; D002478:Cells, Cultured; D037483:Galectin 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D010641:Phenotype; D016548:Prosencephalon / Q000166:cytology / Q000378:metabolism; D040081:Protein Array Analysis; D011485:Protein Binding; D040901:Proteomics / Q000379:methods; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D002241:Carbohydrates; D037483:Galectin 1