title: Human schistosomiasis haematobium: effective diagnosis of active infection using a pair of monoclonal antibodies against soluble egg antigen.
abstract: The present study was designed to prepare monoclonal antibodies (MAbs) against Schistosoma haematobium soluble egg antigen (SEA) with immunodiagnostic potential for urinary schistosomiasis. From a panel of MAbs, a pair of IgG1 MAbs (2D/11C and 10B/2C) specific for S. haematobium SEA was selected. Both MAbs recognized one band with a 42-kDa molecular weight by western blots. The pair of MAbs was employed in sandwich ELISA for the detection of circulating schistosome antigen (CSA), one as antigen-capturing antibody and the other as peroxidase-conjugated antigen-detecting antibody. The lower detection limit of the assay was 1 ng/ml of S. haematobium SEA. The assay was performed on sera of 65 S. haematobium-infected patients, 25 patients infected with other parasites (Fasciola hepatica, Echinococcus granulosus), and 20 noninfected individuals. CSA was demonstrated in 89% of the S. haematobium-infected group. However, CSA was negative in the sera of healthy individuals and patients infected with other parasites, giving an overall specificity of 100% for the CSA assay. A positive correlation (r=0.37, p<0.01) was detected between the number of S. haematobium eggs excreted in 10 ml urine and the CSA level detected in the sera of S. haematobium-infected patients. Our data show that the use of anti-S. haematobium MAbs for the detection of CSA provides a sensitive and specific method for the immunodiagnosis of active S. haematobium-infected patients. Moreover, CSA assay using this anti-S. haematobium MAb/ELISA system was proven to correlate with intensity of infection and hence morbidity assessment.
authors.affiliation: Department of Immunology, Theodor Bilharz Research Institute, Warak El Hadar, Imbaba, Giza, P.O. Box 30, Cairo, 12411, Egypt. ibrahimshalash@yahoo.com
authors.forename: F
authors.initials: F
authors.lastname: Salah
authors.forename: A
authors.initials: A
authors.lastname: El Bassiouny
authors.forename: I
authors.initials: I
authors.lastname: Rabia
authors.forename: Z
authors.initials: Z
authors.lastname: Demerdash
authors.forename: M
authors.initials: M
authors.lastname: Roshdy
authors.forename: Z
authors.initials: Z
authors.lastname: Shaker
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008018:Life Cycle Stages; D051379:Mice; D008807:Mice, Inbred BALB C; D010063:Ovum / Q000276:immunology; D012548:Schistosoma haematobium / Q000254:growth & development / Q000276:immunology; D012553:Schistosomiasis haematobia / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology* / Q000652:urine; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth