title: Prostaglandin E2 release from isolated bladder strips in rats with spinal cord injury.
abstract: OBJECTIVE
  Recent studies have shown that various factors contribute to the increased excitability into the bladder afferent nerves in spinal cord injury (SCI) rats. It has been reported that prostaglandins (PG) act as local modulators of reflex micturition in pathological conditions. In the present study, we measured the amount of PGE2 release from the bladder of chronic SCI rats.
  
  
  METHODS
  Spinal cord was transected at the level of T8-9 in adult female Sprague-Dawley rats. After 10 weeks, specimens of the urinary bladder were obtained from SCI rats and sham-injured control rats, and bladder strips were dissected from the bladder. Using an muscle-bath technique and a microdialysis procedure, the dialysate, containing substance released from bladder strips, was collected. Then the amount of PGE2 in the dialysate was measured by radioimmunoassay.
  
  
  RESULTS
  Excretion of urinary PGE2 was significantly higher in SCI rats than in control rats. PGE2 release from bladder strips was significantly higher in SCI rats than in control rats. Removal of urothelium caused significant decreases in PGE2 release in both control and SCI rats. Stretches of the bladder strips caused significant resting tension-dependent increases in PGE2 release from the strips with urothelium.
  
  
  CONCLUSIONS
  The present data suggest that bladder urothelium partly contributes to the increase in PGE2 release from the bladder, and that bladder distension may cause increases in PGE2 release in SCI rats.
authors.affiliation: Department of Urology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Masunaga
authors.forename: Masaki
authors.initials: M
authors.lastname: Yoshida
authors.forename: Akito
authors.initials: A
authors.lastname: Inadome
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iwashita
authors.forename: Koichi
authors.initials: K
authors.lastname: Miyamae
authors.forename: Shoichi
authors.initials: S
authors.lastname: Ueda
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005500:Follow-Up Studies; D066298:In Vitro Techniques; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000150:complications / Q000378:metabolism*; D001743:Urinary Bladder / Q000294:innervation / Q000378:metabolism* / Q000473:pathology; D014549:Urinary Incontinence / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D019459:Urothelium / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers; D015232:Dinoprostone