Shock wave induced cytoskeletal and morphological deformations in a human renal carcinoma cell line.
Effects of shock waves on the morphology and cytoskeleton of a human renal carcinoma cell line (ACHN) were investigated in vitro. ACHN monolayer cultured on a cover slide glass was treated with 10 shots of focused underwater shock waves, with 16 MPa peak pressure at the focal area of a piezoceramic shock wave generator. After exposure to the shock wave, based on the severity of morphological deformations of the treated cells, the monolayer was divided into three morphological areas; focal, marginal and intact. Morphological deformations were found to be associated with disorganization of the intracellular cytoskeletal filaments. Deformation of the cytoskeletal proteins in the treated cells were separately studied with respect to the location of the cells within the three morphological areas. Among three major cytoskeletal proteins, actin and tubulin, but not vimentin, were affected by the shock waves. The deformed cells reorganized their cytoskeletal network within 3 h with a pattern similar to the control, indicating the transient characteristic of the shock wave induced cytoskeletal damage in the surviving cells. The remaining cell fragments on the slide glass, which contained short actin filaments, indicated the important role of shear stress in damaging the cytoskeletal fibers by shock waves.
Moosavi-Nejad
S Fatemeh
SF
Biomedical Engineering Research Organization, Tohoku University, 2-1-1, Katahira, Aoba, Sendai 980-8577, Japan. nooshin@ceres.ifs.tohoku.ac.jp
Hosseini
S Hamid R
SH
Satoh
Makoto
M
Takayama
Kazuyoshi
K
D000199:Actins / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D006874:Hydrostatic Pressure; D007680:Kidney Neoplasms / Q000378:metabolism / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000199:Actins; D014404:Tubulin