title: Facile synthesis and application of uniformly 13C, 15N-labeled phosphotyrosine for ligand binding studies.
abstract: The first synthesis of [U-13C, 15N] labeled phosphotyrosine is described. Preliminary studies toward the binding of phosphotyrosine to an SH2 domain have been performed by means of heteronuclear NMR.
authors.affiliation: Leiden Institute of Chemistry, Gorlaeus Laboratories, PO Box 9502, 2300 RA Leiden, The Netherlands.
authors.forename: Adriaan W
authors.initials: AW
authors.lastname: Tuin
authors.forename: Gregg
authors.initials: G
authors.lastname: Siegal
authors.forename: Gijsbert A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: Herman S
authors.initials: HS
authors.lastname: Overkleeft
authors.forename: Dmitri V
authors.initials: DV
authors.lastname: Filippov
mesh_terms: D001665:Binding Sites; D002247:Carbon Isotopes; D007553:Isotope Labeling / Q000379:methods; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008956:Models, Chemical; D009587:Nitrogen Isotopes; D019000:Phosphotyrosine / Q000138:chemical synthesis* / Q000378:metabolism; D018909:src Homology Domains / Q000502:physiology
chemical_list: D002247:Carbon Isotopes; D008024:Ligands; D009587:Nitrogen Isotopes; D019000:Phosphotyrosine