title: Applications of highly sensitive phosphopeptide derivatization methods without the need for organic solvents.
abstract: Detection and identification of phosphorylated amino acid residues is still one of the most challenging tasks in contemporary protein analysis. Especially serine- and threonine-phosphorylation is very often involved in the regulation of enzyme activities and regulates key functions in cellular processes.
authors.affiliation: Protein Mass Spectrometry and Functional Proteomics Group, Rudolf-Virchow-Center for Experimental Biomedicine, Julius-Maximilians-Universität Würzburg, Würzburg, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Reinders
authors.forename: Helmut E
authors.initials: HE
authors.lastname: Meyer
authors.forename: Albert
authors.initials: A
authors.lastname: Sickmann
mesh_terms: D002853:Chromatography, Liquid; D003543:Cysteamine / Q000737:chemistry; D003903:Deuterium; D004229:Dithiothreitol / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D010748:Phosphopeptides / Q000032:analysis / Q000737:chemistry*; D010766:Phosphorylation; D012997:Solvents / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D009930:Organic Chemicals; D010748:Phosphopeptides; D012997:Solvents; D003543:Cysteamine; D003903:Deuterium; D004229:Dithiothreitol