Visualization and sensing of intermolecular interactions with two-color fluorescent probes.
We developed a new generic fluorescence sensing technology based on the change of relative intensities between two well-separated emission bands of the novel functional 3-hydroxychromone (3HC) dyes. A greatly enhanced self-calibrating wavelength-ratiometric response is obtained to all major types of non-covalent interactions that can be used in sensing--to polarity, hydrogen bonding ability and to local electrostatic fields. This technology may find a broad range of applications--from homogeneous assays in solutions to microarrays, microfluidic devices, nanosensors and whole cell imaging systems. It allows transforming micelles or phospholipid vesicles into nanosensor devices. In cellular research a high sensitivity to membrane potentials can be obtained and the membrane changes during apoptosis detected.
Demchenko
Alexander P
AP
TUBITAK Research Institute for Genetic Engineering and Biotechnology (RIGEB), Marmara Research Center, 41470 Gebze-Kocaeli, Turkey. dem@gmbae.tubitak.gov.tr
D015374:Biosensing Techniques; D005456:Fluorescent Dyes; D036103:Nanotechnology
D005456:Fluorescent Dyes