Time course analysis of tyrosine hydroxylase and angiotensinogen mRNA expression in central nervous system of rats submitted to experimental hypertension.
Catecholaminergic and angiotensinergic systems are involved in the neural control of blood pressure. The present study analysed the expression of tyrosine hydroxylase (TH), a key enzyme for catecholamine synthesis and of angiotensinogen (AGT), the precursor of angiotensin II (Ang II), in areas of the central nervous system (CNS) involved with cardiovascular regulation such as nucleus tractus solitarius (NTS), ventrolateral medulla (VLM), locus coeruleus (LC) and hypothalamic paraventricular nucleus (PVN) 2 h, 3 and 7 days after aortic coarctated hypertensive rats. In situ hybridization, was employed for the analysis of messenger RNA (mRNA) expression with anatomical resolution. No changes were seen in TH and AGT mRNA expression in the analysed areas 2 h and 3 days after aortic coarctation when compared to the respective sham group. TH mRNA expression was increased in the NTS and LC of rats 7 days after coarctation hypertension when compared to sham rats. Time course analysis, showed an increase in TH mRNA expression in the NTS 7 days after aortic coarctation when compared to 2 h and 3 days groups, as well as an increase in LC 3 days and 7 days following coarctation hypertension in comparison with the 2 h group. Analysis of AGT mRNA in the NTS expression revealed a decrease at 3 days, followed by an increase in mRNA expression 7 days following coarctation hypertension when compared to the sham group. Time course analysis, showed an increase in AGT mRNA expression in the NTS 7 days after coarctation when compared to 2 h and 3 days groups. The results show that TH and AGT mRNA expression changes during the different phases of experimental hypertension, suggesting that the noradrenaline (NOR) and angiotensin II (Ang II) might participate in the modulation/maintenance of coarctation hypertension.
Maximino
J R
JR
Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
Ferrari
M F R
MF
Coelho
E F
EF
Fior-Chadi
D R
DR
D000804:Angiotensin II / Q000096:biosynthesis; D000808:Angiotensinogen / Q000235:genetics*; D000818:Animals; D001017:Aortic Coarctation / Q000150:complications / Q000503:physiopathology; D001794:Blood Pressure / Q000235:genetics; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D001933:Brain Stem / Q000378:metabolism; D002395:Catecholamines / Q000096:biosynthesis; D004195:Disease Models, Animal; D015870:Gene Expression / Q000502:physiology; D006973:Hypertension / Q000235:genetics* / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011921:Rats, Inbred WKY; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
D002395:Catecholamines; D012333:RNA, Messenger; D000808:Angiotensinogen; D000804:Angiotensin II; D014446:Tyrosine 3-Monooxygenase