Involvement of 2-C-methyl-D-erythritol-4-phosphate pathway in biosynthesis of aphidicolin-like tetracyclic diterpene of Scoparia dulcis.
Specific inhibitors of the MVA pathway (pravastatin) and the MEP pathway (fosmidomycin) were used to interfere with the biosynthetic flux which leads to the production of aphidicolin-like diterpene in leaf organ cultures of Scoparia dulcis. Treatment of leaf organs with fosmidomycin resulted in dose dependent inhibition of chlorophylls, carotenoids, scopadulcic acid B (SDB) and phytol production, and no effect on sterol production was observed. In response to the pravastatin treatment, a significant decrease in sterol and perturbation of SDB production was observed.
Nkembo
Marguerite Kasidimoko
MK
Faculty of Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Lee
Jung-Bum
JB
Nakagiri
Takeshi
T
Hayashi
Toshimitsu
T
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D016590:Aphidicolin / Q000096:biosynthesis*; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000378:metabolism*; D004896:Erythritol / Q000031:analogs & derivatives* / Q000378:metabolism; D005578:Fosfomycin / Q000031:analogs & derivatives / Q000494:pharmacology; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000378:metabolism; D017035:Pravastatin / Q000494:pharmacology; D036721:Scoparia / Q000187:drug effects / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013261:Sterols / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism*
C114232:2-C-methylerythritol 4-phosphate; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D004224:Diterpenes; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D013261:Sterols; D013403:Sugar Phosphates; D002734:Chlorophyll; D005578:Fosfomycin; D002338:Carotenoids; D016590:Aphidicolin; C024640:fosmidomycin; D017035:Pravastatin; D004896:Erythritol