title: Analytical, physiologic, and clinical validation of a radioimmunoassay for measurement of procollagen type III amino terminal propeptide in serum and bronchoalveolar lavage fluid obtained from dogs.
abstract: OBJECTIVE
  To validate a radioimmunoassay for measurement of procollagen type III amino terminal propeptide (PIIINP) concentrations in canine serum and bronchoalveolar lavage fluid (BALF) and investigate the effects of physiologic and pathologic conditions on PIIINP concentrations.
  
  
  METHODS
  Sera from healthy adult (n = 70) and growing dogs (20) and dogs with chronic renal failure (CRF; 10), cardiomyopathy (CMP; 12), or degenerative valve disease (DVD; 26); and sera and BALF from dogs with chronic bronchopneumopathy (CBP; 15) and healthy control dogs (10 growing and 9 adult dogs).
  
  
  METHODS
  A radioimmunoassay was validated, and a reference range for serum PIIINP (S-PIIINP) concentration was established. Effects of growth, age, sex, weight, CRF, and heart failure on S-PIIINP concentration were analyzed. In CBP-affected dogs, S-PIIINP and BALF-PIIINP concentrations were evaluated.
  
  
  RESULTS
  The radioimmunoassay had good sensitivity, linearity, precision, and reproducibility and reasonable accuracy for measurement of S-PIIINP and BALF-PIIINP concentrations. The S-PIIINP concentration reference range in adult dogs was 8.86 to 11.48 mug/L. Serum PIIINP concentration correlated with weight and age. Growing dogs had significantly higher S-PIIINP concentrations than adults, but concentrations in CRF-, CMP-, DVD-, or CBP-affected dogs were not significantly different from control values. Mean BALF-PIIINP concentration was significantly higher in CBP-affected dogs than in healthy adults.
  
  
  CONCLUSIONS
  In dogs, renal or cardiac disease or CBP did not significantly affect S-PIIINP concentration; dogs with CBP had high BALF-PIIINP concentrations. Data suggest that the use of PIIINP as a marker of pathologic fibrosis might be limited in growing dogs.
authors.affiliation: Department of Small Animal Clinical Sciences, University of Liège, 4000 Liège, Belgium.
authors.forename: Simone
authors.initials: S
authors.lastname: Schuller
authors.forename: Sophie
authors.initials: S
authors.lastname: Valentin
authors.forename: Benoit
authors.initials: B
authors.lastname: Remy
authors.forename: Pascale
authors.initials: P
authors.lastname: Jespers
authors.forename: Suzanne
authors.initials: S
authors.lastname: Foulon
authors.forename: Nicole
authors.initials: N
authors.lastname: Van Israël
authors.forename: Cécile
authors.initials: C
authors.lastname: Clercx
authors.forename: Kathleen
authors.initials: K
authors.lastname: McEntee
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D001996:Bronchopneumonia / Q000097:blood / Q000662:veterinary; D009202:Cardiomyopathies / Q000097:blood / Q000662:veterinary; D002908:Chronic Disease; D004283:Dog Diseases / Q000097:blood* / Q000378:metabolism*; D004285:Dogs; D005260:Female; D006349:Heart Valve Diseases / Q000097:blood / Q000662:veterinary; D007676:Kidney Failure, Chronic / Q000097:blood / Q000662:veterinary; D008297:Male; D010446:Peptide Fragments / Q000032:analysis* / Q000097:blood; D011347:Procollagen / Q000032:analysis* / Q000097:blood; D011863:Radioimmunoassay / Q000662:veterinary*; D015203:Reproducibility of Results
chemical_list: D010446:Peptide Fragments; D011347:Procollagen; C041876:procollagen Type III-N-terminal peptide