title: Microarray analysis of transcription factor gene expression in melatonin-treated human peripheral blood mononuclear cells.
abstract: The existence of specific melatonin-binding sites in lymphoid cells led to the discovery of signal transduction pathway for melatonin in human lymphocytes and immunomodulatory role of melatonin in immune cells. In recent years, transcriptional regulation of melatonin on various transcription factors has been demonstrated. Therefore, this study was designed to assess by cDNA microarray analysis the regulatory effects of melatonin on transcription factors in human peripheral blood mononuclear cells (PBMCs). Forty-six genes were upregulated and 23 were downregulated more than twofold in melatonin-treated PBMCs. Of the more than twofold upregulated transcription factor genes, homeo box A4 (HOXA4), forkhead box O1A (FOXO1A), transcription elongation factor B (SIII), polypeptide 3 (TCEB3), and peroxisome proliferative activated receptor delta (PPARD) were identified. Of the more than twofold downregulated genes, PHD finger protein 15 (PHF15) and zinc finger protein 33a (ZNF33A) were identified. In summary, identification of these genes by cDNA microarray analysis in response to melatonin administration may provide a foundation for further studies on the function of melatonin in human PBMCs.
authors.affiliation: Department of Biochemisty, Kohwang Medical Research Institute, College of Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Ha
authors.forename: Earl
authors.initials: E
authors.lastname: Han
authors.forename: Hae Jeong
authors.initials: HJ
authors.lastname: Park
authors.forename: Hak-Jae
authors.initials: HJ
authors.lastname: Kim
authors.forename: Mee Suk
authors.initials: MS
authors.lastname: Hong
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Hong
authors.forename: Kyung Sik
authors.initials: KS
authors.lastname: Yoon
authors.forename: Insug
authors.initials: I
authors.lastname: Kang
authors.forename: Yong Hoo
authors.initials: YH
authors.lastname: Cho
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Sung-Vin
authors.initials: SV
authors.lastname: Yim
authors.forename: Hyung Hwan
authors.initials: HH
authors.lastname: Baik
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D017931:DNA Primers; D014157:Transcription Factors