Adhesion receptor expression by CD34+ cells from peripheral blood or bone marrow grafts: correlation with time to engraftment.
OBJECTIVE
This study was undertaken to define the pattern of cell adhesion receptor expression by the CD34+ progenitor cells from mobilized peripheral blood and bone marrow from normal and autologous donors, and to correlate the adhesion receptor profile with time to blood cell recovery for patients undergoing autologous transplant.


METHODS
Blood cell recovery was determined by absolute neutrophil count (> 500/microL), time to last red cell transfusion, and platelet count (> 50,000/microL and > 100,000/microL). The analysis for expression of adhesion receptors alphaL (CD11a), alpha2 (CD49b), alpha3 (CD49c), alpha4 (CD49d), alpha5 (CD49e), alpha6 (CD49f), beta1 (CD29), L-selectin (CD62L), ICAM-1 (CD54), PECAM-1 (CD31), HCAM (CD44), and P-selectin (CD62P) was performed by two-color flow cytometry. The Kruskal-Wallis test and Spearman's rank correlation were used for statistical analysis.


RESULTS
Statistical analysis of adhesion expression by the CD34+ population for patients undergoing autologous transplant demonstrated that the higher percent expression of PECAM-1 correlated with longer time to platelet recovery (> 100,000/microL, p = 0.049). In contrast, the higher the percent expression of alpha6 (p = 0.013) and the increased density of expression of alpha2 (p = 0.035), alpha3 (p = 0.023), alpha4 (p = 0.044), beta1 (p = 0.027), and ICAM-1 (p = 0.010) correlated with shorter time to platelet recovery. Neutrophil recovery time decreased with increased density of expression of alphaL (p = 0.014) and P-selectin (p = 0.007) receptors. Increased density of expression of CD44 (HCAM) was associated with longer time to red blood cell recovery (p = 0.05).


CONCLUSIONS
These data suggest that upregulation of specific adhesion receptors or selection of certain cell populations could result in earlier blood cell recovery after transplant.
Gold
Jack
J
Department of Mathematics, University of Massachusetts, Amherst, MA, USA.
Valinski
Helen M
HM
Hanks
Adrianne N
AN
Ballen
Karen K
KK
Hsieh
Chung-Cheng
CC
Becker
Pamela S
PS
D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D016026:Bone Marrow Transplantation; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009369:Neoplasms / Q000473:pathology / Q000628:therapy; D019038:Platelet Glycoprotein GPIb-IX Complex; D014182:Transplantation, Autologous
D018952:Antigens, CD34; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D019038:Platelet Glycoprotein GPIb-IX Complex; C094990:adhesion receptor