Oligonucleotide microarray analysis of ameloblastoma compared with dentigerous cyst.
BACKGROUND
Ameloblastoma is a benign, but locally invasive tumor known for its high rate of recurrence. However, few comprehensive genetic studies have been conducted about its tumorigenesis. Our aim was to identify possible genes involved in the development and progression of ameloblastoma, using microarray analysis with dentigerous cyst as a control.


METHODS
Total RNA was extracted from two fresh dentigerous cysts and ameloblastoma specimens. Following microarray analysis, semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were performed on selected genes.


RESULTS
Seventy-three genes were overexpressed and 49 were underexpressed. These genes were divided into categories according to function. The microarray results for 13 selected genes were verified with semiquantitative RT-PCR.


CONCLUSIONS
We identified important genes related to the development and progression of ameloblastoma through a large-scale gene expression analysis. This study will stimulate further investigations on genes significant for early diagnosis and prognosis of ameloblastoma.
Lim
Jijun
J
Department of Oral Pathology, Dental School, Dental Research Institute, Seoul National University, Jongno-gu, Seoul, Korea.
Ahn
Hyowon
H
Min
Sunhong
S
Hong
Seong-Doo
SD
Lee
Jae-Il
JI
Hong
Sam-Pyo
SP
D000293:Adolescent; D000328:Adult; D000564:Ameloblastoma / Q000235:genetics* / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis; D003803:Dentigerous Cyst / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008339:Mandibular Neoplasms / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm