Increased number of anaerobic bacteria in the infected root canal in type 2 diabetic rats.
OBJECTIVE
The purpose of this study was to investigate the relationship between type 2 diabetes mellitus and anaerobic bacteria detected in infected root canals.


METHODS
Normal Wistar rats (control) received a standard laboratory diet with water (group A), and GK rats (type 2 diabetes mellitus rats) a normal laboratory diet with water (group B) or a 30% sucrose solution (group C). Chemotaxis assay was conducted on polymorphonuclear leukocytes from the 3 groups, and the numbers of anaerobic bacteria in infected root canals were determined.


RESULTS
In the chemotaxis assay on the polymorphonuclear leukocytes, the chemotactic response of cells in group C was lower than that for groups A and B (P < .01). As to bacteria detected in the root canal, obligate anaerobic bacteria which stained gram negative, were significantly more numerous in group C (P < .01) than in groups A and B.


CONCLUSIONS
The metabolic condition produced by type 2 diabetes mellitus in rats might lower the general host resistance against bacterial infection.
Iwama
Akihiro
A
Department of Endodontics and Research Institute of Advanced Oral Science, School Of Dentistry, Aichi-Gakuin University, Nagoya, Japan. akihiro@dpc.aichi-gakuin.ac.jp
Morimoto
Taisuke
T
Tsuji
Masahito
M
Nakamura
Koki
K
Higuchi
Naoya
N
Imaizumi
Ichiro
I
Shibata
Naoki
N
Yamasaki
Masahiro
M
Nakamura
Hiroshi
H
D000818:Animals; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D001424:Bacterial Infections / Q000150:complications* / Q000276:immunology / Q000382:microbiology; D002634:Chemotaxis, Leukocyte; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003790:Dental Pulp Necrosis / Q000150:complications / Q000276:immunology / Q000382:microbiology*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000276:immunology; D004195:Disease Models, Animal; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D009504:Neutrophils / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar