Reliable new high-performance liquid chromatographic method for the determination of ciprofloxacin in human serum.
Ciprofloxacin is a broad-spectrum antibacterial drug, being used in a wide range of infections. A method to quantify the total (unbound + protein-bound) ciprofloxacin concentrations in human serum is presented based on rapid liquid-liquid extraction, followed by reversed-phase liquid chromatography and UV detection at 280 nm. The calibration range used was 2 to 10 mg/L. The mean accuracy and precision (CV%) were all within specifications: at 0.2 mg/L 81.2% (10.1%), assigned to be the lower limit of quantification (LLOQ), at 8 mg/L 103.6% (4.48%), at 14 mg/L 103.1% (3.98%). Metabolites and frequently used comedications showed minimal interference. Sample storage conditions suitable for routine determination of serum ciprofloxacin concentrations for daily therapeutic drug monitoring (gel-collection tubes and PVC tubes at 4 degrees C) and for intermittent batch-wise analyses for research purposes (PVC tubes at -18 degrees C) showed acceptable sample stability after storage for 48 hours (4 degrees C) and 6 months (-18 degrees C).
van Geijlswijk
Ingeborg M
IM
Department of Hospital Pharmacy, Gelderse Vallei Hospital, Ede, The Netherlands. geijlswijki@zgv.nl
van Zanten
Arthur R H
AR
van der Meer
Y Geert
YG
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000031:analogs & derivatives / Q000097:blood* / Q000378:metabolism; D016903:Drug Monitoring / Q000379:methods; D004355:Drug Stability; D024841:Fluoroquinolones / Q000097:blood / Q000378:metabolism; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature; D013997:Time Factors
D024841:Fluoroquinolones; C054029:ciprofloxacin-7-ethylenediamine; C060059:oxociprofloxacin; C060058:sulfociprofloxacin; D002939:Ciprofloxacin