Whole genome analysis of non-optimal codon usage in secretory signal sequences of Streptomyces coelicolor.
Non-optimal (rare) codons have been suggested to reduce translation rate and facilitate secretion in Escherichia coli. In this study, the complete genome analysis of non-optimal codon usage in secretory signal sequences and non-secretory sequences of Streptomyces coelicolor was performed. The result showed that there was a higher proportion of non-optimal codons in secretory signal sequences than in non-secretory sequences. The increased tendency was more obvious when tested with the experimental data of secretory proteins from proteomics analysis. Some non-optimal codons for Arg (AGA, CGU and CGA), Ile (AUA) and Lys (AAA) were significantly over presented in the secretary signal sequences. It may reveal that a balanced non-optimal codon usage was necessary for protein secretion and expression in Streptomyces.
Li
Yu-Dong
YD
Zhejiang University, College of Life, Hangzhou, PR China.
Li
Yong-Quan
YQ
Chen
Jian-shu
JS
Dong
Hui-jun
HJ
Guan
Wen-Jun
WJ
Zhou
Hong
H
D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D019295:Computational Biology; D016680:Genome, Bacterial / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics*; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism*
D003062:Codon; D021382:Protein Sorting Signals