Pectin-like acidic polysaccharide from Panax ginseng with selective antiadhesive activity against pathogenic bacteria.
Previous studies have revealed the inhibitory effects of an acidic polysaccharide purified from the root of Panax ginseng against the adhesion of Helicobacter pylori to gastric epithelial cells and the ability of Porphyromonas gingivalis to agglutinate erythrocytes. In this study, this acidic polysaccharide from P. ginseng, PG-F2, was investigated further, in order to characterize its antiadhesive effects against Actinobacillus actinomycetemcomitans, Propionibacterium acnes, and Staphylococcus aureus. The minimum inhibitory concentrations (MIC) were found to be in a range of 0.25-0.5mg/mL. However, results showed no inhibitory effects of PG-F2 against Lactobacillus acidophilus, Escherichia coli, or Staphylococcus epidermidis. PG-F2 is a pectin-type polysaccharide with a mean MW of 1.2 x 10(4) Da, and consists primarily of galacturonic and glucuronic acids along with rhamnose, arabinose, and galactose as minor components. The complete hydrolysis of PG-F2 via chemical or carbohydrolase enzyme treatment resulted in the abrogation of its antiadhesive activity, but limited hydrolysis via treatment with pectinase (EC. 3.2.1.15) yielded an oligosaccharide fraction, with activity comparable to the precursor PG-F2 (the MIC of ca. 0.01 mg/mL against H. pylori and P. gingivalis). Our results suggest that PG-F2 may exert a selective antiadhesive effect against pathogenic bacteria, while having no effects on beneficial and commensal bacteria.
Lee
Ji-Hye
JH
Department of Food Technology, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Shim
Jin Sun
JS
Lee
Jung Sun
JS
Kim
Mi-Kyung
MK
Chung
Mi-Sook
MS
Kim
Kyung Hyun
KH
D001419:Bacteria / Q000472:pathogenicity*; D001422:Bacterial Adhesion / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D005894:Panax; D010368:Pectins / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000502:physiology*; D014774:Virulence / Q000502:physiology
D011134:Polysaccharides; D010368:Pectins