title: Nuclear pores form de novo from both sides of the nuclear envelope.
abstract: Nuclear pore complexes are multiprotein channels that span the double lipid bilayer of the nuclear envelope. How new pores are inserted into the intact nuclear envelope of proliferating and differentiating eukaryotic cells is unknown. We found that the Nup107-160 complex was incorporated into assembly sites in the nuclear envelope from both the nucleoplasmic and the cytoplasmic sides. Nuclear pore insertion required the generation of Ran guanosine triphosphate in the nuclear and cytoplasmic compartments. Newly formed nuclear pore complexes did not contain structural components of preexisting pores, suggesting that they can form de novo.
authors.affiliation: Salk Institute for Biological Studies, Molecular and Cell Biology Laboratory, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Maximiliano A
authors.initials: MA
authors.lastname: D'Angelo
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Anderson
authors.forename: Erin
authors.initials: E
authors.lastname: Richard
authors.forename: Martin W
authors.initials: MW
authors.lastname: Hetzer
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D002474:Cell-Free System; D003593:Cytoplasm / Q000378:metabolism; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006160:Guanosine Triphosphate / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D009685:Nuclear Envelope / Q000378:metabolism* / Q000648:ultrastructure*; D022022:Nuclear Pore / Q000378:metabolism* / Q000648:ultrastructure*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014909:Wheat Germ Agglutinins / Q000378:metabolism / Q000494:pharmacology; D014981:Xenopus; D028961:beta Karyopherins / Q000378:metabolism / Q000494:pharmacology; D020931:ran GTP-Binding Protein / Q000378:metabolism
chemical_list: D028861:Nuclear Pore Complex Proteins; D011994:Recombinant Proteins; D014909:Wheat Germ Agglutinins; D028961:beta Karyopherins; D004533:Egtazic Acid; D006160:Guanosine Triphosphate; D020931:ran GTP-Binding Protein; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid