Polymorphism of DRD2 gene and ADHD.
OBJECTIVE
Attention deficit hyperactivity disorder (ADHD) is a prevalent childhood disorder. Evidence from the family and twin studies suggest that ADHD is familiar and highly heritable. Association studies are frequently used for the searching of markers responsible for genetic basis of ADHD. We investigated TaqI polymorphism of the dopamine receptor D2 (DRD2) in relationship with ADHD. The association between TaqI A polymorphism of DRD2 gene and ADHD has previously been published.


METHODS
We used the association study to test the relationship between TaqI A polymorphism of DRD2 gene and ADHD on groups of ADHD boys and control boys.


METHODS
For DNA isolation, buccal tissue was used. PCR with restriction analysis of PCR products was used for genotyping.


RESULTS
We found statistically different genotypic and allelic frequencies (p < 0.008, p < 0.002, respectively) of DRD2 polymorphism between two studied groups of boys.


RESULTS
According to our results we suppose that polymorphism TaqI A of DRD2 gene is involved in the pathogenesis of childhood ADHD in male subjects. Allele A1 and genotype A1A1 in male subjects is associated with ADHD.


CONCLUSIONS
Our study confirmed the relationship between TaqI A polymorphism of DRD2 gene and ADHD published previously.
Serý
Omar
O
Laboratory of neurobiology and molecular psychiatry, Department of Biochemistry, Masaryk University, Faculty of Science, Brno, Czech Republic. omarsery@sci.muni.cz
Drtílková
Ivana
I
Theiner
Pavel
P
Pitelová
Renáta
R
Staif
Radim
R
Znojil
Vladimír
V
Lochman
Jan
J
Didden
William
W
D000293:Adolescent; D000483:Alleles; D001289:Attention Deficit Disorder with Hyperactivity / Q000453:epidemiology* / Q000235:genetics* / Q000523:psychology; D002648:Child; D018153:Czech Republic / Q000453:epidemiology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005787:Gene Frequency; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D011569:Psychiatric Status Rating Scales; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017448:Receptors, Dopamine D2; D004247:DNA