[Inhibin B and intraacrosomal proteins in men from the couples with fertility disorders].
OBJECTIVE
To monitor the basic andrologic and immunologic sperm factors and the levels of inhibin B in serum and in seminal plasma in men from the couples with infertility disorders.


METHODS
Department of Gynecology and Obstetrics, Medical School, Charles University and University Hospital, Plzen, Institute of Molecular Genetics, AV CR, Prague, Institute of Clinical Immunology and Allergology, LF UK a FN, Plzen.


METHODS
We used conventional methods for estimation of sperm quality according to WHO and we detected the intra-acrosomal proteins by monoclonal antibodies (Hs8 and Hs14, immunofluorescent method), spermantibodies by direct mixed antiimunoglobulin reaction (MAR) test, and we examined inhibin B in serum (< or =400 pg/ml= A) and in seminal plasma (< or = 600 pg/ml= N) by ELISA in 355 men aged 21-52 years (ø 34 years) with normal levels of FSH, LH and testosterone. The control group was created by 56 health sperm donors.


RESULTS
We found 65% normospermatics in the group of 355 patients, 34.9% men with various kind of pathologies. Predominance of spermagglutinating antibodies was found in 15.77% in IgG, in 19.44% in IgA, in 8.44% in IgA and IgG together. Normal intraacrosomal proteins were reached in 74.65% for Hs8, in 20.85% pathologic, in 86.2% normal findings for Hs14, in 4.23% pathologic. The immunological results in control group were completaly negative. Pathological levels of inhibin B in seminal plasma was found in 37.2% (152 men), in 25% in serum, and in 5.6% in serum and in seminal plasma together. In 54.7% of patients we found physiological levels of inhibin B in both biological fluids. We also compared physiological 109/152 (71.71%), and pathological spermiogrammes 43/152 (28.29%) with abnormal levels of inhibin B in seminal plasma, with intraacrosomal proteins to levels of inhibin B in serum. Our detailed study shows high interidividual results, which must be studied in complex with diagnosis of decreased fertility in man.


CONCLUSIONS
Andrologic and immunologic analysis in the group of 355 men showed normal parameters of spermiogrammes in 231 patients (65%), in the rest of men the immunologic profil was in various parts pathologic. Only 105 men have got excellent spermiogrammes. Inhibin B as hormon regulates in back the secretion of FSH, and serves as good indicator in male reproductive failures.
Babcová
K
K
Gynekologicko-porodnická klinika LF UK a FN, Plzen.
Ulcová-Gallová
Z
Z
Cervenková
Z
Z
Peknicová
J
J
Panzner
P
P
Micanová
Z
Z
Bibková
K
K
Rokyta
Z
Z
D000177:Acrosome / Q000737:chemistry*; D000328:Adult; D001323:Autoantibodies / Q000032:analysis; D006801:Humans; D007248:Infertility, Male / Q000276:immunology / Q000378:metabolism*; D007265:Inhibins / Q000032:analysis* / Q000097:blood; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000032:analysis*; D012661:Semen / Q000737:chemistry*; D013073:Sperm Agglutination / Q000276:immunology; D013076:Sperm Count; D013094:Spermatozoa / Q000276:immunology
D001323:Autoantibodies; D011506:Proteins; C422364:inhibin B; D007265:Inhibins