Engagement of PSGL-1 enhances beta(2)-integrin-involved adhesion of neutrophils to recombinant ICAM-1.
OBJECTIVE
The interactions of selectins and their ligands initiate the process of leukocyte migrating into inflamed tissue. P-selectin glycoprotein ligand 1 (PSGL-1) is the best characterized ligand of selectins, and has been demonstrated to mediate the adhesion of leukocytes to all three selectins in vivo. PSGL-1 not only functions as an anchor molecule to capture the leukocytes to the activated endothelial cells by its interaction with selectins, but also transduces the signals to activate leukocytes. Our present work aimed to investigate the mechanism by which PSGL-1-mediated signal activates neutrophils and enhances the adhesion to the endothelial cells.


METHODS
We detected the effects of the engagement of PSGL-1 with monoclonal antibodies (mAb) or P-selectin on the adhesion of neutrophils to the recombinant intercellular adhesion molecule-1 (ICAM-1), and on the expression of beta(2)-integrin. Additionally, the role of cytoskeleton in these process was studied by using inhibitor cytochalasin B.


RESULTS
The engagement of PSGL-1 increased the expression of beta(2)-integrin on the surface of neutrophils and enhanced the adhesion of neutrophils to the recombinant ICAM-1. mAb against CD18 impaired the adhesion of PSGL-1-engaged neutrophils to ICAM-1. Moreover, the inhibitor cytochalasin B largely blocked the increase of CD18 expression as well as the adhesion of PSGL-1-engaged neutrophils to ICAM-1.


CONCLUSIONS
The PSGL-1-transduced signals can enhance beta(2)-integrin-involved adhesion of neutrophils to the recombinant ICAM-1, and this process depends on the dynamics of cytoskeleton.
Wang
Xiao-Guang
XG
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
Cheng
Yan-Ping
YP
Ba
Xue-Qing
XQ
D018821:CD18 Antigens / Q000378:metabolism* / Q000502:physiology; D002448:Cell Adhesion; D002469:Cell Separation; D003571:Cytochalasin B / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*
D018821:CD18 Antigens; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D018799:Intercellular Adhesion Molecule-1; D003571:Cytochalasin B