title: Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy.
abstract: The signal and idler beams from a picosecond, synchronously pumped optical parametric oscillator (OPO) provide the two colors necessary for coherent anti-Stokes Raman scattering (CARS) microscopy. The OPO provides a continuously tunable frequency difference between the two beams over a broad range of Raman shifts (100-3700 cm(-1)) by varying the temperature of a single nonlinear crystal. The near-infrared output (900-1300 nm) allows for deep penetration into thick samples and reduced nonlinear photodamage. Applications of this light source to in vivo cell and ex vivo tissue imaging are demonstrated.
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Massachusetts 02138, USA.
authors.forename: Feruz
authors.initials: F
authors.lastname: Ganikhanov
authors.forename: Silvia
authors.initials: S
authors.lastname: Carrasco
authors.forename: X
authors.initials: X
authors.lastname: Sunney Xie
authors.forename: Mordechai
authors.initials: M
authors.lastname: Katz
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Seitz
authors.forename: Daniel
authors.initials: D
authors.lastname: Kopf
mesh_terms: D000818:Animals; D004431:Ear, External / Q000166:cytology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007834:Lasers; D008029:Lighting / Q000295:instrumentation*; D051379:Mice; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods