[Protective effect of vitamin C on protein activity in plasma during virus inactivation].
To determine whether addition of vitamin C (Vit C) to single-unit plasma could influence the efficacy of inactivating viruses and could maintain the activity of plasma proteins by methylene blue (MB)-light treatment. Vesicular stomatitis virus (VSV) Indiana strain was used as the indicating virus. Human plasma containing VSV was added with different concentrations of Vit C and final concentration 1 micromol/L MB and irradiated by fluorescence at an intensity of 40,000 lx, samples were collected at different times for detection. Cytopathic effect was used to test the effect of virus inactivation. A segment of the nucleic acid encoding capsid protein of VSV was amplified with RT-PCR. Some methods, such as the Clauss method, the one-stage method, microimmunoelectrophoresis, were used to investigate the changes of plasma components. The results showed that when the VSV plasma was added with 240 micromol/L Vit C and treated by MB-light irradiation for 60 min, the titer of VSV decreased by more than 8 lg TICD50/ml. Meanwhile, target segment amplification of VSV was also negative. The recovery rates of fibrinogen and coagulation factor VIII (FVIII: C) were 83.55% and 81.67% respectively, which had significant difference comparing with the routine MB-fluorescent light treatment. Most of plasma proteins were not affected significantly. No change in immunogenicity of these proteins was observed by using microimmunoelectrophoresis. It is concluded that virus inactivation is not influenced and plasma proteins are effectively protected by Vit C. Vit C can be used as a protector and is beneficial to improving the quality of plasma subjected to MB- photodynamic treatment.
Li
Yan
Y
Departmnet of Microbiology, West China College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Li
Ming-Yuan
MY
Jiang
Ren-Ju
RJ
Jia
Wen-Xiang
WX
D001205:Ascorbic Acid / Q000494:pharmacology*; D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology; D010949:Plasma / Q000821:virology; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects*; D038301:Virus Inactivation / Q000187:drug effects*
D001798:Blood Proteins; D001205:Ascorbic Acid; D008751:Methylene Blue