title: Classification of human liver transplant recipients by their preoperative CD8+ T cell subpopulation and its relation to outcome.
abstract: The primed status of T cells is markedly different among liver transplant recipients, due to a lifetime of antigen exposure and reduced thymopoiesis by aging, and diseases. This study aims to characterize the preoperative immunological status of CD8+ T cell subpopulations and relate it to the outcome for liver transplant recipients. We classified 112 liver transplant recipients into 5 groups, based on hierarchical clustering of the CD8+CD45 isoform proportion of T cells. In Groups I and II (pediatric), the naive T cell proportion was more than 50%. In adult recipients, Group III was characterized by a naive T cell proportion of 50%, Group IV had the greatest effector/memory T cells (EM), and Group V had the greatest proportion of effector T cells. In Groups IV and V, the effector T cell proportion was considerably higher, and was accompanied by marked downregulation of the CD27+CD28+ subsets and upregulation of interferon gamma (IFN)-gamma, tumor necrosis factor-alpha, and perforin expression. Group V recipients tended to be complicated postoperatively, with a significantly reduced survival rate (1 yr, 66.8%) and markedly reduced Eastern Cooperative Oncology Group performance status.
authors.affiliation: Department of Transplantation and Immunology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanaka
authors.forename: Kazue
authors.initials: K
authors.lastname: Ozawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Teramukai
authors.forename: Yasutsugu
authors.initials: Y
authors.lastname: Takada
authors.forename: Hiroto
authors.initials: H
authors.lastname: Egawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kaihara
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ogura
authors.forename: Mureo
authors.initials: M
authors.lastname: Kasahara
authors.forename: Masako
authors.initials: M
authors.lastname: Ono
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sato
authors.forename: Kenji
authors.initials: K
authors.lastname: Takai
authors.forename: Masanori
authors.initials: M
authors.lastname: Fukushima
authors.forename: Nagahiro
authors.initials: N
authors.lastname: Minato
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000096:biosynthesis; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000032:analysis; D016031:Liver Transplantation / Q000009:adverse effects / Q000145:classification / Q000276:immunology*; D019520:Living Donors; D008875:Middle Aged; D011183:Postoperative Complications / Q000209:etiology
chemical_list: D016207:Cytokines; D017493:Leukocyte Common Antigens