title: Uncoupling the pleiotropic phenotypes of clk-1 with tRNA missense suppressors in Caenorhabditis elegans.
abstract: clk-1 encodes a demethoxyubiquinone (DMQ) hydroxylase that is necessary for ubiquinone biosynthesis. When Caenorhabditis elegans clk-1 mutants are grown on bacteria that synthesize ubiquinone (UQ), they are viable but have a pleiotropic phenotype that includes slowed development, behaviors, and aging. However, when grown on UQ-deficient bacteria, the mutants arrest development transiently before growing up to become sterile adults. We identified nine suppressors of the missense mutation clk-1(e2519), which harbors a Glu-to-Lys substitution. All suppress the mutant phenotypes on both UQ-replete and UQ-deficient bacteria. However, each mutant suppresses a different subset of phenotypes, indicating that most phenotypes can be uncoupled from each other. In addition, all suppressors restore the ability to synthesize exceedingly small amounts of UQ, although they still accumulate the precursor DMQ, suggesting that the presence of DMQ is not responsible for the Clk-1 phenotypes. We cloned six of the suppressors, and all encode tRNA(Glu) genes whose anticodons are altered to read the substituted Lys codon of clk-1(e2519). To our knowledge, these suppressors represent the first missense suppressors identified in any metazoan. The pattern of suppression we observe suggests that the individual members of the tRNA(Glu) family are expressed in different tissues and at different levels.
authors.affiliation: Department of Biology, McGill University, 1205 Ave. Docteur Penfield, Montreal, Quebec, Canada H3A 1B1.
authors.forename: Robyn
authors.initials: R
authors.lastname: Branicky
authors.forename: Phuong Anh Thi
authors.initials: PA
authors.lastname: Nguyen
authors.forename: Siegfried
authors.initials: S
authors.lastname: Hekimi
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D016153:Genes, Suppressor; D008239:Lysine / Q000378:metabolism; D020125:Mutation, Missense; D010641:Phenotype; D012352:RNA, Transfer, Glu / Q000235:genetics*; D014451:Ubiquinone / Q000096:biosynthesis
chemical_list: C104339:CLK-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D012352:RNA, Transfer, Glu; D014451:Ubiquinone; D008239:Lysine