title: The importance of RNA structure in RNA editing and a potential proofreading mechanism for correct guide RNA:pre-mRNA binary complex formation.
abstract: RNA editing in trypanosomes is a post-transcriptional process responsible for correcting the coding sequences of many mitochondrial mRNAs. Uridine bases are specifically added or deleted from mRNA by an enzymatic cascade in which a pre-edited mRNA is cleaved specifically, uridine bases are added or removed, and the corrected mRNA is ligated. The process is directed by RNA molecules, termed guide RNAs (gRNA). The ability of this class of small, non-coding RNA to function in RNA editing is essential for these organisms. Typically, gRNAs are transcribed independent of their cognate mRNA and anneal to form a binary RNA complex. An exception from this process is the cytochrome oxidase subunit II (COII) mRNA, which encodes its gRNA within its 3' untranslated region. This gRNA lacks the ability to function in trans. Using an in vitro editing assay, we find that improving thermodynamic stability to the anchor region through increased Watson-Crick base-pairing is sufficient to impart trans editing activity. We further show that a point mutation outside the known functional regions of a gRNA induces both a conformational rearrangement of the gRNA and causes a decrease in the rate of editing. Taken together, these results lead us to propose a model for a potential proofreading step in the formation of a gRNA:pre-edited mRNA binary complex. The mechanism relies on the thermodynamic stability supplied to the RNA complex through Watson-Crick base-pairing. Through mutations in the gRNA, we demonstrate the importance of gRNA structure to the RNA editing reaction.
authors.affiliation: Program in Global Infectious Diseases, Josephine Bay Paul Center, Marine Biological Laboratory, Woods Hole, MA 02543, USA.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Golden
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Hajduk
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D003576:Electron Transport Complex IV / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D017393:RNA Editing; D012322:RNA Precursors / Q000737:chemistry*; D017394:RNA, Guide, Kinetoplastida / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016053:RNA, Protozoan / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013816:Thermodynamics; D014346:Trypanosoma brucei brucei / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D012322:RNA Precursors; D016053:RNA, Protozoan; D003576:Electron Transport Complex IV; D017394:RNA, Guide, Kinetoplastida