title: [Contamination with Sphingomonas paucimobilis: about seven cases isolated in conservation and transport mediums of corneal grafts].
abstract: From September to December 2004, contaminations were found in fifteen conservation and transport mediums of corneal grafts at the tissue bank of Brest, including seven by Sphingomonas paucimobilis. The pulsed-field gel electrophoresis made it possible to establish the genotypic profiles of each strain and to compare them. Similarities were found between certain strains of the contaminated mediums and those of the thermostated double boiler of the tissue bank. The link between the contamination and the defrosting of the mediums in the double boiler was thus established. Measures of prevention are currently proposed to defrost the bottles like the use of a dry bath to replace the current one.
authors.affiliation: Laboratoire d'hygiène hospitalière, CHU Morvan, 29609 Brest, France.
authors.forename: C
authors.initials: C
authors.lastname: Bourigault
authors.forename: L
authors.initials: L
authors.lastname: Daniel
authors.forename: S
authors.initials: S
authors.lastname: Jourdain
authors.forename: E
authors.initials: E
authors.lastname: Hardy
authors.forename: K
authors.initials: K
authors.lastname: Heriaud
authors.forename: M
authors.initials: M
authors.lastname: Virmaux
authors.forename: B
authors.initials: B
authors.lastname: Eniafe-Eveillard
authors.forename: B
authors.initials: B
authors.lastname: Lejeune
mesh_terms: D015373:Bacterial Typing Techniques; D002809:Chondroitin Sulfates; D045424:Complex Mixtures; D003315:Cornea; D016895:Culture Media, Serum-Free; D003911:Dextrans; D016521:Electrophoresis, Gel, Pulsed-Field; D004866:Equipment Contamination; D005839:Gentamicins; D006358:Hot Temperature; D006801:Humans; D011309:Preservation, Biological / Q000379:methods*; D013048:Specimen Handling; D020578:Sphingomonas / Q000145:classification / Q000302:isolation & purification*; D020858:Tissue and Organ Harvesting; D014871:Water Microbiology
chemical_list: D045424:Complex Mixtures; D016895:Culture Media, Serum-Free; D003911:Dextrans; D005839:Gentamicins; D002809:Chondroitin Sulfates; C075736:Optisol