Influence of propolis water solution on heart mitochondrial function.
The effect of propolis water solution (PWS) on the respiration of rat heart mitochondria with NAD-linked (pyruvate + malate), FAD-linked (succinate) substrates and fatty acids (palmitoyl-L-carnitine) was investigated in this study. PWS at the lowest concentration of 4 microg mL(-1) of phenolic compounds (PC) had no effect on mitochondrial respiration with all investigated substrates. PWS at concentrations of 63 and 125 microg mL(-1) of PC caused a significant decrease of basal (24 and 54%) and maximal (58 and 70%) respiration rates with succinate as substrate. At these PWS concentrations the oxidation of pyruvate + malate and palmitoyl-L-carnitine was diminished to a lower degree: the basal respiration rate decreased by 13-18% and the maximal respiration rate by 15-28%. Succinate oxidation was affected, probably because of the inhibition of succinate dehydrogenase by the 1,2-benzenedicarboxylic acid esters found in PWS. The PWS-caused decrease in the mitochondrial respiration rate with pyruvate + malate and fatty acids could be due to diminished activities of respiratory chain complexes and/or ADP/ATP translocator.
Majiene
Daiva
D
Institute for Biomedical Research, Kaunas University of Medicine, Eiveniu str.4, LT-50009 Kaunas-7, Lithuania. daivamaj@vector.kmu.lt
Trumbeckaite
Sonata
S
Savickas
Arunas
A
Toleikis
Adolfas
A
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D066298:In Vitro Techniques; D008293:Malates / Q000378:metabolism; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D010172:Palmitoylcarnitine / Q000378:metabolism; D011429:Propolis / Q000494:pharmacology*; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions; D019802:Succinic Acid / Q000378:metabolism; D014867:Water
D008293:Malates; D012996:Solutions; D014867:Water; D010172:Palmitoylcarnitine; C030298:malic acid; D019289:Pyruvic Acid; D011429:Propolis; D019802:Succinic Acid