A DNA-binding copper(I) metallosupramolecular cylinder that acts as an artificial nuclease.
The DNA binding of a dicationic pyridylimine-based dicopper(I) metallosupramolecular cylinder is reported together with its ability to act as an artificial nuclease. The cylinder binds strongly to DNA; more strongly than the spherical dication [Ru(phen)(3)](2+) (phen=1,10-phenanthroline), but more weakly than the corresponding tetracationic cylinders. DNA coiling effects are not observed with this dication, in contrast to the situation with the previously reported tetracationic cylinder involving a similar ligand. Linear dichroism (LD) data suggests that the dicopper cylinder binds in a different orientation from that of the tetracationic iron cylinder. Furthermore, the dicopper cylinder shows DNA-cleavage activity in the presence of peroxide. Of particular note is that the cylinder displays a marked and unusual ability to cleave both DNA strands at the same site, probably reflecting its dinuclear nature and possibly its mode of binding to the DNA.
Childs
Laura J
LJ
Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.
Malina
Jaroslav
J
Rolfsnes
Britt Elin
BE
Pascu
Mirela
M
Prieto
Maria J
MJ
Broome
Mark J
MJ
Rodger
P Mark
PM
Sletten
Einar
E
Moreno
Virtudes
V
Rodger
Alison
A
Hannon
Michael J
MJ
D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage; D003851:Deoxyribonucleases / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*
D009942:Organometallic Compounds; D011725:Pyridines; D003300:Copper; D004247:DNA; D003851:Deoxyribonucleases