title: Entamoeba histolytica: inflammatory process during amoebic liver abscess formation involves cyclooxygenase-2 expression in macrophages and trophozoites.
abstract: It has been demonstrated that expression of cyclooxygenase-2 (COX-2) isoform is induced by Entamoeba histolytica in macrophages and polymorphonuclear cells during amoebic liver abscess (ALA) formation in hamsters. Trophozoites present in the lesion were also positive for COX-2 signal. However, no cross reactivity of the anti-COX-2 antibody with protein extract of cultivated trophozoites was found. To clarify if trophozoites are involved in PGE(2) production during ALA development, COX-2 expression was detected by in situ hybridization and RT-PCR in liver tissue from intrahepatically infected hamsters. COX-2 mRNA was in polymorphonuclear cells since 4h postinfection, and subsequently, local macrophages expressed COX-2 mRNA in a similar way. Additionally, a positive signal for COX-2 mRNA expression was detected in E. histolytica trophozoites, suggesting that, in vivo, parasite COX expression may be an important mechanism to promote inflammation.
authors.affiliation: Laboratorio de Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Apdo. Postal 1542-C, Chihuahua, Chih., Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Gutiérrez-Alarcón
authors.forename: M
authors.initials: M
authors.lastname: Moguel-Torres
authors.forename: O
authors.initials: O
authors.lastname: Mata-Leyva
authors.forename: G
authors.initials: G
authors.lastname: Cuellar-Nevárez
authors.forename: T
authors.initials: T
authors.lastname: Siqueiros-Cendón
authors.forename: G
authors.initials: G
authors.lastname: Erosa
authors.forename: E
authors.initials: E
authors.lastname: Ramos-Martínez
authors.forename: P
authors.initials: P
authors.lastname: Talamás-Rohana
authors.forename: B
authors.initials: B
authors.lastname: Sánchez-Ramírez
mesh_terms: D000818:Animals; D006224:Cricetinae; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics; D015342:DNA Probes / Q000592:standards; D015232:Dinoprostone / Q000096:biosynthesis; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006790:Host-Parasite Interactions; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007249:Inflammation / Q000201:enzymology / Q000469:parasitology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology / Q000469:parasitology / Q000473:pathology; D008101:Liver Abscess, Amebic / Q000201:enzymology / Q000469:parasitology* / Q000473:pathology; D008264:Macrophages / Q000201:enzymology / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D053058:Trophozoites / Q000201:enzymology
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D015232:Dinoprostone