title: Bactobilia after liver transplantation: frequency and antibiotic susceptibility.
abstract: After liver transplantation (LT), bactobilia occurs frequently in patients, leading in some cases to cholangitis and biliary sepsis. The present study is the first to investigate bactobilia after LT, and it gives an overview of predisposing factors for bactobilia, the microbial spectrum in the bile of LT patients, and the antibiotic susceptibility. A total of 172 endoscopic retrograde cholangiography (ERC) procedures were performed in 66 LT patients between 1 month and 5.8 years after LT. Bile samples were examined microbiologically. Sixty-eight nontransplanted patients without cholestasis, but requiring ERC for other reasons served as a control group. Of 172 samples obtained from LT patients, 126 (73.3%) were positive for microbes. A total of 236 organisms were isolated: 114 (48.3%) gram-positive bacteria, 92 (39.0%) aerobic gram-negative, 8 (3.4%) anaerobes, and 22 (9.3%) fungi. Ciprofloxacin and amoxycillin/clavulanic acid showed the best susceptibility results among oral antibiotics and piperacillin/tazobactam and imipenem/cilastatin among intravenous preparations. In contrast, only 15.7% of non-LT patients showed bactobilia. In conclusion, our study shows that bactobilia is a problem in patients after LT and that it is not only a contamination from endoscopic intervention. Mechanical obstruction, plastic stents, gallstones, and papillotomy increase the risk of bactobilia significantly. In our cohort we had the best antibiotic susceptibility results for positive cultures in LT patients with piperacillin/tazobactam, ciprofloxacin, or amoxycillin/clavulanic acid.
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Innsbruck, Innsbruck, Austria. gunda.millonig@uibk.ac.at
authors.forename: Gunda
authors.initials: G
authors.lastname: Millonig
authors.forename: Thomas
authors.initials: T
authors.lastname: Buratti
authors.forename: Ivo W
authors.initials: IW
authors.lastname: Graziadei
authors.forename: Hubert
authors.initials: H
authors.lastname: Schwaighofer
authors.forename: Dorothea
authors.initials: D
authors.lastname: Orth
authors.forename: Raimund
authors.initials: R
authors.lastname: Margreiter
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Vogel
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification*; D001646:Bile / Q000382:microbiology*; D002758:Cholangiography; D002761:Cholangitis / Q000209:etiology; D005260:Female; D006801:Humans; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged
chemical_list: D000900:Anti-Bacterial Agents