Growth arrest and decrease of alpha-SMA and type I collagen expression by palmitic acid in the rat hepatic stellate cell line PAV-1.
Liver fibrosis is characterized by an activation of hepatic stellate cells (HSC). During primary culture HSC evolve from a quiescent into an activated phenotype which is characterized by alpha-smooth muscle actin (alpha-SMA) up-regulation, increase in cell growth, and extracellular matrix secretion. HSC culture with trans-resveratrol can lead to deactivation of myofibroblast-like HSC. We used an HSC line, PAV-1, to check the role of retinol and palmitic acid in the deactivation process of HSC. Using mass and metabolic-based methods, Western blot and immunocytochemistry assays, we demonstrated that treatment with palmitic acid (75 muM) alone or in combination with retinol (2 muM) significantly decreased cell proliferation and alpha-SMA expression. We also established that the association of both compounds strongly decreased collagen type I expression. Our results suggest the potential use of palmitic acid alone or in combination with retinol to induce HSC deactivation.
Abergel
Armand
A
Unité des Maladies Métaboliques et Micro-nutriments, Equipe Vitamines, INRA de Clermont-Fd-Theix, 63122 Saint-Genès, Champanelle, France. aabergel@chu-clermontferrand.fr
Sapin
Vincent
V
Dif
Nicolas
N
Chassard
Christophe
C
Darcha
Claude
C
Marcand-Sauvant
Julie
J
Gaillard-Martinie
Brigitte
B
Rock
Edmond
E
Dechelotte
Pierre
P
Sauvant
Patrick
P
D000199:Actins / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008103:Liver Cirrhosis; D019308:Palmitic Acid / Q000494:pharmacology*; D051381:Rats; D014801:Vitamin A / Q000494:pharmacology*
D000199:Actins; D024042:Collagen Type I; D019308:Palmitic Acid; D014801:Vitamin A; C498862:Acta2 protein, rat