Overexpression of carnation S-adenosylmethionine decarboxylase gene generates a broad-spectrum tolerance to abiotic stresses in transgenic tobacco plants.
Polyamines (PAs), such as putrescine, spermidine, and spermine, are present in all living organism and implicate in a wide range of cellular physiological processes. We have used transgenic technology in an attempt to evaluate their potential for mitigating the adverse effects of several abiotic stresses in plants. Sense construct of full-length cDNA for S-adenosylmethionine decarboxylase (SAMDC), a key enzyme in PA biosynthesis, from carnation (Dianthus caryophyllus L.) flower was introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium tumefaciens-mediated transformation. Several transgenic lines overexpressing SAMDC gene under the control of cauliflower mosaic virus 35S promoter accumulated soluble total PAs by 2.2 (S16-S-4) to 3.1 (S16-S-1) times than wild-type plants. The transgenic tobacco did not show any difference in organ phenotype compared to the wild-type. The number and weight of seeds increased, and net photosynthetic rate also increased in transgenic plants. Stress-induced damage was attenuated in these transgenic plants, in the symptom of visible yellowing and chlorophyll degradation after all experienced stresses such as salt stress, cold stress, acidic stress, and abscisic acid treatment. H2O2-induced damage was attenuated by spermidine treatment. Transcripts for antioxidant enzymes (ascorbate peroxidase, manganase superoxide dismutase, and glutathione S-transferase) in transgenic plants and GUS activity transformed with SAMDC promoter::GUS fusion were induced more significantly by stress treatment, compared to control. These results that the transgenic plants with sense SAMDC cDNA are more tolerant to abiotic stresses than wild-type plants suggest that PAs may play an important role in contributing stress tolerance in plants.
Wi
Soo Jin
SJ
Department of Biology, College of Natural Science, Sunchon National University, Sunchon, Chonnam 540-742, Korea.
Kim
Woo Taek
WT
Park
Ky Young
KY
D000222:Adaptation, Physiological / Q000187:drug effects; D012437:Adenosylmethionine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D029749:Dianthus / Q000201:enzymology*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D005966:Glucuronidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008190:Lyases / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology; D018515:Plant Leaves / Q000187:drug effects; D030821:Plants, Genetically Modified; D011073:Polyamines / Q000032:analysis; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D014170:Transformation, Genetic
D000975:Antioxidants; D018076:DNA, Complementary; D011073:Polyamines; D012333:RNA, Messenger; D006861:Hydrogen Peroxide; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase; D005966:Glucuronidase; D008190:Lyases; D012437:Adenosylmethionine Decarboxylase; C021773:1-aminocyclopropanecarboxylate synthase