title: Experimental design considerations in quantification experiments by using the internal standard technique: cholesterol determination by gas chromatography as a case study.
abstract: An overview on experimental design in quantification experiments using the internal standard technique is described. Particular emphasis is given to the modelling of the relationship analyte/internal standard and the estimation of the optimal amount of internal standard to be used throughout the analytical concentration range by using a uniform shell design with a minimum number of seven experiments. The described approach is demonstrated in the analysis of cholesterol in various reference materials by gas chromatography (GC), using alpha-5 cholestan as internal standard. The outlined approaches can be applied in the determination of any kind of analyte.
authors.affiliation: National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 2029 Nordnes, N-5817 Bergen, Norway. pedro.araujo@nifes.no
authors.forename: Pedro
authors.initials: P
authors.lastname: Araujo
authors.forename: Felicia
authors.initials: F
authors.lastname: Couillard
authors.forename: Else
authors.initials: E
authors.lastname: Leirnes
authors.forename: Kjersti
authors.initials: K
authors.lastname: Ask
authors.forename: Annbjørg
authors.initials: A
authors.lastname: Bøkevoll
authors.forename: Livar
authors.initials: L
authors.lastname: Frøyland
mesh_terms: D002784:Cholesterol / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D012015:Reference Standards
chemical_list: D002784:Cholesterol