Mass spectrometry strategies for venom mapping and peptide sequencing from crude venoms: case applications with single arthropod specimen.
Due to their complexity and diversity, animal venoms represent an extensive source of bioactive compounds such as peptides and proteins. Conventional approaches for their characterization often require large quantities of biological material. Current mass spectrometry (MS) techniques now give access to a wealth of information in a short working time frame with minute amounts of sample. Such MS approaches may now be used for the discovery of novel compounds, and once their structure has been determined they may be synthesized and tested for functional activity. Molecular mass fingerprints of venoms allow the rapid identification of known toxins as well as preliminary structural characterization of new compounds. De novo peptide sequencing by tandem mass spectrometry (MS/MS) offers rapid access to partial or total primary peptide structures. This article, written as a tutorial, also contains new material: molecular mass fingerprint analysis of Orthochirus innesi scorpion venom, and identification of components from bumblebee Bombus lapidarius venom, both collected from one single specimen. The structure of the three major peptides detected in the Bombus venom was fully characterized in one working day by de novo sequencing using an electrospray ionization hybrid quadrupole time-of-flight instrument (ESI-QqTOF) and a matrix-assisted laser desorption ionization time-of-flight instrument (MALDI-LIFT-TOF-TOF). After presenting the MS-based sequence elucidation, perspectives in using MS and MS/MS techniques in toxinology are discussed.
Favreau
Philippe
P
Atheris Laboratories, Research and Development, Case postale 314, CH-1233 Bernex-Geneva, Switzerland.
Menin
Laure
L
Michalet
Sophie
S
Perret
Fréderic
F
Cheneval
Olivier
O
Stöcklin
Maxime
M
Bulet
Philippe
P
Stöcklin
Reto
R
D000818:Animals; D001514:Bee Venoms / Q000032:analysis*; D001516:Bees; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D040901:Proteomics; D012604:Scorpion Venoms / Q000032:analysis*; D012605:Scorpions / Q000737:chemistry
D001514:Bee Venoms; D012604:Scorpion Venoms