title: Loss of heterozygosity analysis of a candidate gastric carcinoma tumor suppressor locus at 7q31.
abstract: Gastric carcinoma is one of the most common malignancies in Asia. Although the allelic deletion of 7q has been reportedly associated with primary gastric carcinoma tumorigenesis, no predisposing genes in this region have been identified so far. Here, we report the results of genotype and loss of heterozygosity (LOH) analysis on 7q in this tumor. A panel of nine microsatellite markers distributed over the whole chromosome 7q was used for genotyping primary gastric carcinomas. Of 72 primary tumors LOH of D7S486 occurred in 24.0% (12/50) of cases. Fine mapping with 12 additional markers flanking D7S486 resulted in LOH of 30.36% (17/56) and defined one minimal deleted region in primary gastric carcinomas, a 90-kilobase region bounded by D7S2543 and D7S486 at 7q31.2. The allelic deletion correlates statistically with clinicopathologic variables. Our data suggest a possible link between putative tumor suppressor genes and gastric carcinoma in the 7q31 region.
authors.affiliation: Research Section of Oncopathology, State Key Laboratory of Oncology in Southern China, Cancer Center, Zhongshan University, 651 Dong-feng Road East, Guangzhou 510060, China.
authors.forename: Jian-Chuan
authors.initials: JC
authors.lastname: Xia
authors.forename: De-Sheng
authors.initials: DS
authors.lastname: Weng
authors.forename: Jin-Tian
authors.initials: JT
authors.lastname: Li
authors.forename: Hai-De
authors.initials: HD
authors.lastname: Qin
authors.forename: Shi-Juan
authors.initials: SJ
authors.lastname: Mai
authors.forename: Bing-Jian
authors.initials: BJ
authors.lastname: Feng
authors.forename: Qin
authors.initials: Q
authors.lastname: Fan
authors.forename: Qi-Sheng
authors.initials: QS
authors.lastname: Feng
authors.forename: Li-Xi
authors.initials: LX
authors.lastname: Huang
authors.forename: Xing-Juan
authors.initials: XJ
authors.lastname: Yu
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Pan
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Li
authors.forename: Qi-Jing
authors.initials: QJ
authors.lastname: Wang
authors.forename: You-Qing
authors.initials: YQ
authors.lastname: Zhan
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: He
authors.forename: Wen-lin
authors.initials: WL
authors.lastname: Huang
authors.forename: Pei-Hong
authors.initials: PH
authors.lastname: Wu
authors.forename: Yi-Xing
authors.initials: YX
authors.lastname: Zeng
mesh_terms: D000328:Adult; D000368:Aged; D002872:Chromosome Deletion; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D020161:Physical Chromosome Mapping; D013274:Stomach Neoplasms / Q000235:genetics*