Polymorphism of the PE domain of PE/PE_PGRS sequences in clinical isolates of Mycobacterium bovis in Mexico.
Polymorphism of the PE domain of PE/PE_PGRS sequences was studied in Mycobacterium bovis isolates from different Mexican states. Samples were analyzed by spolygotyping and RFLP using IS6110 and a 235-bp fragment of the PE domain of PE/PE_PGRS as probes. With the PE probe, three different genotypes were observed, one being predominant in all states. These results confirm the high conservation of the PE domain and suggests a potential role for PE sequence as a stable genetic marker for bovine tuberculosis.
Santillan-Flores
Marco Antonio
MA
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, CENID-Microbiología, Mexico City, Mexico.
Flores
Javier
J
Arriaga-Diaz
Camila
C
Romero-Torres
Cecilia
C
Suárez-Güemes
Francisco
F
Espitia
Clara
C
D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D005819:Genetic Markers; D005838:Genotype; D008800:Mexico; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D014380:Tuberculosis, Bovine / Q000175:diagnosis
D017931:DNA Primers; D004269:DNA, Bacterial; D005819:Genetic Markers