Stimulation of pneumovirus-specific CD8+ T-cells using a non-toxic recombinant ricin delivery system.
Internalisation of the plant toxin ricin occurs by retrograde transport which delivers the toxin to the ER where it intersects with the MHC class I system for peptide antigen display. Here, we describe the generation of an inactivated, non-toxic, ricin molecule fused to a peptide which elicits a CD8+ T-cell response in mice directed against pneumonia virus of mice, a pneumovirus related to human respiratory syncytial virus. The ricin fusion elicited a significant T-cell response when delivered by intraperitoneal inoculation in the absence of adjuvent. Challenge experiments showed that the T-cell response resulting from inoculation with the ricin-peptide fusion molecule delayed the onset of virus-induced disease.
Grimaldi
Elizabeth
E
Department of Biological Science, University of Warwick, Coventry CV4 7AL, UK.
Claassen
Erwin A W
EA
Michael Lord
J
J
Smith
Daniel C
DC
Easton
Andrew J
AJ
D000818:Animals; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D005260:Female; D006027:Glycosides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D018111:Pneumovirus / Q000235:genetics / Q000276:immunology; D018186:Pneumovirus Infections / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage*; D012276:Ricin / Q000008:administration & dosage*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014315:Triterpenes / Q000494:pharmacology; D014764:Viral Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
D018984:Epitopes, T-Lymphocyte; D006027:Glycosides; D010455:Peptides; D011993:Recombinant Fusion Proteins; D014315:Triterpenes; D014764:Viral Proteins; C001883:stichoposide; D012276:Ricin