title: Hydride transfer versus hydrogen radical transfer in thymidylate synthase.
abstract: The nature of a H-transfer in the thymidylate synthase catalyzed reaction was investigated by comparison of the wild-type enzyme with the W80M mutant. The nature of the H-transfer was not affected, as indicated by intrinsic isotope effects and their temperature dependence. These findings support a single-step hydride transfer instead of a two-step radical transfer.
authors.affiliation: Department of Chemistry, University of Iowa, Iowa City, Iowa 52242-1294, USA.
authors.forename: Baoyu
authors.initials: B
authors.lastname: Hong
authors.forename: Majd
authors.initials: M
authors.lastname: Haddad
authors.forename: Frank
authors.initials: F
authors.lastname: Maley
authors.forename: Jan H
authors.initials: JH
authors.lastname: Jensen
authors.forename: Amnon
authors.initials: A
authors.lastname: Kohen
mesh_terms: D006859:Hydrogen / Q000737:chemistry*; D013940:Thymidylate Synthase / Q000737:chemistry*
chemical_list: D006859:Hydrogen; D013940:Thymidylate Synthase