Purification of potato virus X and preparation of its antiserum.
Potato virus X (PVX) isolated from the potato leaf and tuber samples which were collected from various fields in Damavand and Ardabil. The initial isolations of the virus were made from potato by mechanical inoculation on Gomphrena globosa L. and Chenopodium spp. that produce local lesion, and then it causes mosaic on Nicotiana spp. and Datura stramonium L. An isolate of the virus inoculated to Nicotiana glutinosa L. and it was maintained throughout the work. Sap from infected N. glutinosa was ineffective after dilution to 10-6, 10 minutes at 70 degrees and 10 weeks at room temperature. The virus was readily purified from infected leaves and the best protocol was Moreira & Jones 1980 than the other 2 methods of Fribourg 1975 and Shepard & Shalla 1972. Antisera were prepared against native, degraded proteins and micro precipitin test showed that both antisera had a 1/512 titer. Precipitin lines with D - Protein antiserum was better of the native protein antiserum in agar double diffusion test than treated with SDS. The isolate of the virus was not transmitted by none of 2 species of Cuscuta but transmitted from infected leaves to healthy plants with sap inoculation without using Carburandum. This isolate showed positive reaction with gamaglubulin in kate received from CIP centre.
Damadi
S M
SM
Plant Protection of Tehran, Karaj, Iran.
Mosahebi
G H
GH
Okhovvat
M
M
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007106:Immune Sera; D007492:Iran; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D017863:Potexvirus / Q000302:isolation & purification*; D011233:Precipitin Tests / Q000379:methods; D011198:Solanum tuberosum / Q000821:virology*
D007106:Immune Sera