title: Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly-N-acetyllactosamine antigens.
abstract: Poly-N-acetyllactosamines (pLNs) are common terminal sugars of many N- and O-linked glycan structures present in glycoproteins and glycolipids. Utilizing various glycosyltransferases, we developed new and efficient chemoenzymatic methods for the synthesis of pLNs in gram-scale. Specifically, the use of sialyltransferases and fucosyltransferases enabled us to synthesize and purify 24 blood group and tumor-associated pLN derivatives with alpha-(2-->3)- and alpha-(2-->6)-linked sialic acid, as well as with alpha-(1-->2)- and alpha-(1-->3)-linked fucose. All synthesized derivatives were linked to a short 2-azidoethyl spacer for further modification.
authors.affiliation: Glycan Array Synthesis Core D, Consortium for Functional Glycomics. The Scripps Research Institute, Department of Molecular Biology, CB 248A 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Daniela
authors.initials: D
authors.lastname: Vasiliu
authors.forename: Nahid
authors.initials: N
authors.lastname: Razi
authors.forename: Yingning
authors.initials: Y
authors.lastname: Zhang
authors.forename: Nathan
authors.initials: N
authors.lastname: Jacobsen
authors.forename: Kirk
authors.initials: K
authors.lastname: Allin
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Liu
authors.forename: Julia
authors.initials: J
authors.lastname: Hoffmann
authors.forename: Ognian
authors.initials: O
authors.lastname: Bohorov
authors.forename: Ola
authors.initials: O
authors.lastname: Blixt
mesh_terms: D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D001789:Blood Group Antigens / Q000096:biosynthesis*; D005647:Fucosyltransferases / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis / Q000138:chemical synthesis*; D012799:Sialyltransferases / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D001789:Blood Group Antigens; D011134:Polysaccharides; C037199:poly-N-acetyllactosamine; D005647:Fucosyltransferases; D012799:Sialyltransferases