title: Effect of iron on the activation of the MAPK/ERK pathway in PC12 neuroblastoma cells.
abstract: Recent evidence suggests that reactive oxygen species function as second messenger molecules in normal physiological processes. For example, activation of N-Methyl-D-Aspartate receptor results in the production of ROS, which appears to be critical for synaptic plasticity, one of the cellular mechanisms that underlie learning and memory. In this work, we studied the effect of iron in the activation of MAPK/ERK pathway and on Ca2+ signaling in neuronal PC12 cells. We found that iron-dependent generation of hydroxyl radicals is likely to modulate Ca2+ signaling through RyR calcium channel activation, which, in turn, activates the MAPK/ERK pathway. These findings underline the relevance of iron in normal neuronal function.
authors.affiliation: PhD Program in Cell, Molecular and Neural Biology, Faculty of Sciences, Universidad de Chile. pabluesmen@yahoo.com
authors.forename: Pablo
authors.initials: P
authors.lastname: Muñoz
authors.forename: Gabriela
authors.initials: G
authors.lastname: Zavala
authors.forename: Karen
authors.initials: K
authors.lastname: Castillo
authors.forename: Pabla
authors.initials: P
authors.lastname: Aguirre
authors.forename: Cecilia
authors.initials: C
authors.lastname: Hidalgo
authors.forename: Marco T
authors.initials: MT
authors.lastname: Núñez
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects* / Q000378:metabolism; D007501:Iron / Q000494:pharmacology*; D009447:Neuroblastoma / Q000201:enzymology*; D016716:PC12 Cells / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D019837:Ryanodine Receptor Calcium Release Channel / Q000187:drug effects* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D017382:Reactive Oxygen Species; D019837:Ryanodine Receptor Calcium Release Channel; D007501:Iron; D048049:Extracellular Signal-Regulated MAP Kinases