Increased angiogenesis and angiogenic gene expression in carotid artery plaques from symptomatic stroke patients.
OBJECTIVE
Carotid plaque rupture is one of the main causes of stroke by creating cerebral emboli. The biochemical, molecular, and structural factors that promote carotid plaque rupture are not yet understood in detail. We hypothesize that increased microvascular blood flow within a carotid plaque might fissure the plaque, elevate local pressure, and promote plaque rupture. The aim of this study is to determine the role of angiogenesis and angiogenesis-related gene expression in symptomatic carotid plaque.


METHODS
The present study evaluated the new vessel formation (using hematoxylin-eosin staining and CD34 immunohistochemistry) and angiogenic gene expression (using microarray and real-time polymerase chain reaction analysis) in carotid plaque specimens obtained during endarterectomy from 13 symptomatic stroke patients in comparison with eight asymptomatic patients.


RESULTS
Symptomatic plaques showed significantly higher new vessel density in the fibrous cap (by 347%, P < 0.05) as well as in the plaque proper (by 196%, P < 0.05) compared with the asymptomatic plaques. The fibrous caps of the plaques were threefold thinner in the symptomatic patients when compared with the asymptomatic patients. In symptomatic plaque, gene expression analysis showed increased abundance of 31 transcripts known to promote angiogenesis and cell division compared with plaques of asymptomatic patients.


CONCLUSIONS
This study suggests that angiogenic gene expression and the ensuing angiogenesis in the plaques might contribute to their destabilization and resulting symptoms.
Türeyen
Kudret
K
Department of Neurological Surgery, University of Wisconsin, Madison, Wisconsin 53792-3232, USA.
Vemuganti
Raghu
R
Salamat
M Shahriar
MS
Dempsey
Robert J
RJ
D000328:Adult; D000368:Aged; D016893:Carotid Stenosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016894:Endarterectomy, Carotid / Q000379:methods; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000502:physiology*; D020521:Stroke / Q000235:genetics / Q000378:metabolism* / Q000473:pathology