Comparison of the fragmentation pattern induced by collisions, laser excitation and electron capture. Influence of the initial excitation.
Collision-induced dissociation, laser-induced dissociation and electron-capture dissociation are compared on a singly and doubly protonated pentapeptide. The dissociation spectrum depends on the excitation mechanism and on the charge state of the peptide. The comparison of these results with the conformations obtained from Monte Carlo simulations suggests that the de-excitation mechanism following a laser or an electron-capture excitation is related to the initial geometry of the peptide.
Antoine
R
R
Laboratoire de Spectrométrie Ionique et Moléculaire, UMR 5579 (Université Lyon I et CNRS), 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
Broyer
M
M
Chamot-Rooke
J
J
Dedonder
C
C
Desfrançois
C
C
Dugourd
P
P
Grégoire
G
G
Jouvet
C
C
Onidas
D
D
Poulain
P
P
Tabarin
T
T
van der Rest
G
G
D003198:Computer Simulation; D004583:Electrons; D007259:Infrared Rays; D007834:Lasers; D008958:Models, Molecular; D009010:Monte Carlo Method; D009842:Oligopeptides / Q000737:chemistry* / Q000528:radiation effects*; D011487:Protein Conformation; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009842:Oligopeptides