title: [Preparation and characterization of monoclonal antibody against DCXR].
abstract: OBJECTIVE
  To prepare monoclonal antibodies (mAbs) against Dicarbonyl/L-xylulose reductase (DCXR).
  
  
  METHODS
  Normal human liver tissues were homogenized, and mitochondria were isolated by differential centrifugation. The total mitochondrial proteins were used to immunize BALB/c mice to prepare mAbs by routine hybridoma technique. The mAbs were detected by ELISA, Western blot and immunohistochemistry. The antibody specificities were identified by mass spectrometry (MS) following immunoprecipitation (IP) and confirmed by Uni-ZAP expression library screening.
  
  
  RESULTS
  One clone of hybridoma AFF091 secreting specific mAb against DCXR was obtained. The Ig subclass of the mAb was IgG1 and it can be used in ELISA, Western blot, immunohistochemistry, and immunoprecipitation.
  
  
  CONCLUSIONS
  A hybridoma cell line stably secreting specific mAb against DCXR was established. The specific mAb against DCXR would be very useful for investigation of DCXR functions and distribution.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China. yanfangju@yahoo.com.cn
authors.forename: Yan-fang
authors.initials: YF
authors.lastname: Ju
authors.forename: Jin-ju
authors.initials: JJ
authors.lastname: Yang
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Liu
authors.forename: Jian-en
authors.initials: JE
authors.lastname: Gao
authors.forename: Qi-hong
authors.initials: QH
authors.lastname: Sun
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D015153:Blotting, Western; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013401:Sugar Alcohol Dehydrogenases / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D013401:Sugar Alcohol Dehydrogenases; C023300:L-xylulose reductase