Molecular identification of tropical tasar silkworm (Antheraea mylitta) ecoraces with RAPD and SCAR markers.
The tropical tasar silkworm, Antheraea mylitta, has several ecoraces, 10 of which are commercially exploited for the production of tasar silk. These ecoraces are identified by morphological markers that are greatly influenced by photoperiod, humidity, altitude, and host plants. The DNA markers, random amplification of polymorphic DNA (RAPD), and sequence-characterized amplified region (SCAR) are identified to complement the existing morphological markers. Seven RAPD bands are selected that identify 8 of the 10 ecoraces. These identified RAPD fragments are sequenced and primers are designed for SCAR markers. Of the seven sets of primers, a single primer pair produced polymorphic SCAR bands that diagnose 5 of the 10 ecoraces. All 10 ecoraces are identified by the use of RAPD and SCAR markers together.
Saha
Monalee
M
Department of Biotechnology, Indian Institute of Technology, Kharagpur, 721302, India.
Kundu
S C
SC
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D005819:Genetic Markers; D009036:Moths / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D005819:Genetic Markers