title: Peptide ligands targeting integrin alpha3beta1 in non-small cell lung cancer.
abstract: Lung cancer is one of the most common cancers and is the leading cause of cancer death. We wish to identify peptide ligands for unique cell surface receptors of non-small lung cancer with the hope of developing these ligands as diagnostic and therapeutic agents. Using the method of 'one-bead one-peptide' combinatorial chemistry, a library of random cyclic octapeptides was synthesized on polystyrene beads. This library was used to screen for peptides that promoted attachment of lung adenocarcinoma cells employing a 'cell-growth-on-bead' assay. Consensus peptide sequences of cNGXGXXc were identified. These peptides promoted cell adhesion by targeting integrin alpha3beta1 over-expressed in non-small lung cancer cells. These peptide beads can be applied to capture cancer cells in malignant pleural fluid for purpose of diagnosis of lung cancer.
authors.affiliation: Division of Hematology/Oncology, Department of Internal Medicine, University of California, Davis, Cancer Center and Northern California VA Health System, 4501 X Street, Sacramento, CA 95817, USA. derick.lau@ucdmc.ucdavis.edu
authors.forename: Derick
authors.initials: D
authors.lastname: Lau
authors.forename: Linlang
authors.initials: L
authors.lastname: Guo
authors.forename: Ruiwu
authors.initials: R
authors.lastname: Liu
authors.forename: Jan
authors.initials: J
authors.lastname: Marik
authors.forename: Kit
authors.initials: K
authors.lastname: Lam
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D039201:Integrin alpha3beta1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008024:Ligands; D008175:Lung Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology*; D019151:Peptide Library
chemical_list: D039201:Integrin alpha3beta1; D008024:Ligands; D009363:Neoplasm Proteins; D009842:Oligopeptides; D019151:Peptide Library