title: [Polymorphisms of HCMV US28 gene of the clinical isolates from children].
abstract: BACKGROUND
  To study the polymorphism of human cytomegalovirus US28 gene in children and investigate the relationship between the polymorphism and pathogenesis.
  
  
  METHODS
  The FQ-PCR was carried out to determine the DNA quantity of clinical isolate and then the segmental PCR and HMA-SSCP were performed to test the mutation of US28 gene. The typical isolates from different diseases were selected to clone and sequence, then the results were analyzed.
  
  
  RESULTS
  The nucleic acid mutation is frequent among the sequence of US28, those mutations focus on the two ends of US28, but most of them are sense mutation. The important functional groups of US28 are highly conserved. The amino acid mutation of some isolates resulted in the change of secondary structure, but the phylogenetic tree analysis did not show any clear association between the pathogenesis and the distribution of clinical isolates. The comparison of US28 sequences from AIDS patients with the sequences from children in our study showed that both sequences have their own specific high mutation points.
  
  
  CONCLUSIONS
  There is polymorphism among the HCMV-US28 gene of clinical isolates from children. There observed no clear relationship was between the pathogenesis and the distribution of clinical isolates.
authors.affiliation: The Virology Laboratory of The National Key Laboratory, Ministry of Health for Congenital Malformation Research. The Second Affiliated Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Xia
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
authors.forename: Jin-jin
authors.initials: JJ
authors.lastname: Guo
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Yao-hua
authors.initials: YH
authors.lastname: Ji
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: Wei
authors.forename: Shu-rong
authors.initials: SR
authors.lastname: Chen
authors.forename: Lan-qing
authors.initials: LQ
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D002648:Child; D000013:Congenital Abnormalities / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D019707:Receptors, Chemokine / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D019707:Receptors, Chemokine; C411191:US28 receptor, Cytomegalovirus; D014764:Viral Proteins