title: Genetic and epigenetic analysis of the TIMP-3 gene in ovarian cancer.
abstract: Chromosome 22q shows a high frequency of loss of heterozygosity (LOH) in ovarian cancers suggesting the existence of one or more important tumor suppressor genes (TSGs). The tissue inhibitor of metalloproteinase-3 (TIMP-3) is a plausible TSG candidate since it is often encompassed within these regions of LOH. TIMP-3 has not previously been investigated for somatic mutations or promoter hypermethylation in ovarian cancer. We analyzed 65 ovarian cancers for both somatic genetic mutations and TIMP-3 promoter hypermethylation. Screening of all coding exons of TIMP-3 did not reveal any somatic genetic mutations and only 1/65 showed TIMP-3 methylation. Our data indicate that inactivation of TIMP-3 by somatic mutation or promoter hypermethylation is rare in ovarian cancer.
authors.affiliation: Cancer Genetics Laboratory, Victorian Breast Cancer Research Consortium, Peter MacCallum Cancer Centre, St Andrews Place, East Melbourne, Vic, Australia.
authors.forename: Mira C P
authors.initials: MC
authors.lastname: Liu
authors.forename: David Y H
authors.initials: DY
authors.lastname: Choong
authors.forename: Christine S F
authors.initials: CS
authors.lastname: Hooi
authors.forename: Louise H
authors.initials: LH
authors.lastname: Williams
authors.forename: Ian G
authors.initials: IG
authors.lastname: Campbell
mesh_terms: D001483:Base Sequence; D002892:Chromosomes, Human, Pair 22; D019175:DNA Methylation; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D008969:Molecular Sequence Data; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics*; D000097792:Tissue Inhibitor of Metalloproteinase-4
chemical_list: D019714:Tissue Inhibitor of Metalloproteinases; D000097792:Tissue Inhibitor of Metalloproteinase-4