title: [Characteristics of autologous peripheral blood stem cells in pediatric patients].
abstract: Autologous peripheral blood stem cells are increasingly used for transplantation instead of bone marrow, even in small children and adolescents. We analyzed 73 autologous leukaphereses performed in 25 children (36% males, 64% females). The median age was 15 years (range 3-18) and the median body weight 48 kg (range 16-67). The apheresis procedures were carried out with cell separator COBE Spectra. Each patient underwent a median of 2 collections (range 1-6). The median total nucleated cell yield was 11.86x108/kg (range 1.94-21.21), mononuclear cell yield was 6.01x10(8)/kg (range 0.97-12.73) and CD34+ cell yield was 3.5 x10(6)/kg (range 0.19-28.01). During 6 (8.22%) procedures the patients had experienced apheresis-related side effects. The citrate-induced reactions were most commonly observed. The reactions were mild and cessation of collection was required only in one case, because of catheter related complication. Our results show that leukapheresis in pediatric patients is a safe procedure, well tolerated and with a very low risk of serious adverse events.
authors.affiliation: Zavod za klinicku transfuziologiju, Klinicki zavod za laboratorijsku dijagnostiku, KBC Zagreb. ibojanic@kbc-zagreb.hr
authors.forename: Ines
authors.initials: I
authors.lastname: Bojanić
authors.forename: Branka
authors.initials: B
authors.lastname: Golubić Cepulić
authors.forename: Damir
authors.initials: D
authors.lastname: Nemet
authors.forename: Ljubica
authors.initials: L
authors.lastname: Rajić
authors.forename: Drago
authors.initials: D
authors.lastname: Batinić
authors.forename: Boris
authors.initials: B
authors.lastname: Labar
mesh_terms: D000293:Adolescent; D002469:Cell Separation; D002648:Child; D002675:Child, Preschool; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000166:cytology*; D006801:Humans; D007937:Leukapheresis; D008297:Male; D014182:Transplantation, Autologous