Urinary pseudouridine in patients with lymphoma: comparison with other clinical parameters.
BACKGROUND
Some patients with malignant lymphoma do not manifest superficial lymphadenopathy. In such cases, clinical parameters that indicate the number of tumor cells are important for the assessment of tumor growth and choice of proper treatment. We evaluated urinary pseudouridine (U-PU) as an indicator of the growth of malignant lymphoma by comparing its levels with serum concentrations of other clinical parameters in patients with various lymphomas at various stages.


METHODS
Urine was obtained from 67 patients with lymphoma. U-PU was assayed by recombinant Fab-based inhibition ELISA. Serum soluble IL2 receptor (sIL2R), serum deoxythymidine kinase (dTK), serum beta-2 microglobulin (beta2MG) and serum lactate dehydrogenase (LDH) were also assayed.


RESULTS
U-PU concentrations showed good correlations with serum concentrations of beta2MG, LDH, sIL2R and dTK. The level of U-PU was higher in stage IV than in stages I (P=0.023), II (P=0.006) and III (P=0.036).


CONCLUSIONS
U-PU concentration correlates with the clinical stage of lymphoma and is a useful tool to assess the growth of lymphoma.
Masaki
Yasufumi
Y
Hematology and Immunology, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Kahoku-gun, Ishikawa 920-0293, Japan. yasum@kanazawa-med.ac.jp <yasum@kanazawa-med.ac.jp>
Itoh
Kunihiko
K
Sawaki
Toshioki
T
Karasawa
Hiromi
H
Kawanami
Takafumi
T
Fukushima
Toshihiro
T
Kawabata
Hiroshi
H
Wano
Yuji
Y
Hirose
Yuko
Y
Suzuki
Toshio
T
Sugai
Susumu
S
Umehara
Hisanori
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008223:Lymphoma / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011560:Pseudouridine / Q000652:urine*; D015375:Receptors, Interleukin-2 / Q000097:blood; D013937:Thymidine Kinase / Q000097:blood; D001613:beta 2-Microglobulin / Q000097:blood
D014408:Biomarkers, Tumor; D015375:Receptors, Interleukin-2; D001613:beta 2-Microglobulin; D011560:Pseudouridine; D007770:L-Lactate Dehydrogenase; D013937:Thymidine Kinase