title: Sulfasalazine prevents apoptosis in spermatogenic cells after experimental testicular torsion/detorsion.
abstract: OBJECTIVE
  To determine whether sulfasalazine can prevent apoptosis in spermatogenic cells by preventing the activation of NF-kappaB in spermatogenic epithelium in experimental testicular torsion.
  
  
  METHODS
  Thirty-two adult male Sprague-Dawley rats were subjected to unilateral 720 degree testicular torsion for durations of 0 h and 2 h, then the torsion was relieved. The ischemic/reperfused testes were collected for the detection of NF-kappaB expression with Western blotting and immunohistochemistry techniques, and detection of apoptosis with TUNEL techniques.
  
  
  RESULTS
  The NF-kappaB coefficient of spermatogenic epithelium and the apoptosis index of spermatogenic cells were significantly different in the operation and the sham-operation groups after experimental testicular torsion (P<0.01).
  
  
  CONCLUSIONS
  NF-kappaB activation of spermatogenic epithelium is related to apoptosis of spermatogenic cells. Sulfasalazine can prevent apoptosis in spermatogenic cells after the experimental testicular torsion through prevention of NF-kappaB activation.
authors.affiliation: Department of Urology, Xiangfan Central Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Xiangfan 441021, China. zhaoyugang@21cn.com
authors.forename: Yu-Gang
authors.initials: YG
authors.lastname: Zhao
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Zheng
authors.forename: Ji
authors.initials: J
authors.lastname: Zhou
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Liu
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Hu
authors.forename: De-Hong
authors.initials: DH
authors.lastname: Chen
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Men
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Shi
authors.forename: Zhi-Yun
authors.initials: ZY
authors.lastname: Yu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012670:Seminiferous Epithelium / Q000166:cytology / Q000378:metabolism; D013086:Spermatic Cord Torsion / Q000378:metabolism / Q000473:pathology*; D013090:Spermatocytes / Q000166:cytology* / Q000378:metabolism; D012460:Sulfasalazine / Q000494:pharmacology*
chemical_list: D016328:NF-kappa B; D012460:Sulfasalazine