title: [Etiology of aseptic meningitis prevalent in Xuzhou].
abstract: BACKGROUND
  To find the pathogenic agents of aseptic meningitis prevalent in Xuzhou of Jiangsu province in 2001.
  
  
  METHODS
  The enterovirus (EV) was cultured from CSF of the patients and identified with anti-serum by neutralization test. Neutralization titer of antibody in paired sera from meningitis children was determined. EV RNA was detected by RT-PCR.
  
  
  RESULTS
  Four strains of Coxsackievirus B5, 2 strains of Coxsackievirus B3 and 1 strain of Echovirus 7 were isolated from 22 CSF specimens. The isolation rate of virus was 31.8% (7/22), 21 CSF were tested by RT-PCR, the positive rate of EV RNA was 52.4% (11/21); 57.9% (11/19) of patients paired-sera had over 4 folds antibody rise or became seroconverted.
  
  
  CONCLUSIONS
  Enterovirus was the pathogenic agent of aseptic meningitis prevalent in Xuzhou of Jiangsu province, the main serotype of the virus was Coxsackievirus B5.
authors.affiliation: Jiangsu Province Center for Disease Control and Prevention, Nanjing 210009, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Xi-ling
authors.initials: XL
authors.lastname: Guo
authors.forename: Zhi-yang
authors.initials: ZY
authors.lastname: Shi
authors.forename: Da-xin
authors.initials: DX
authors.lastname: Ni
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003384:Coxsackievirus Infections / Q000134:cerebrospinal fluid / Q000453:epidemiology / Q000821:virology; D004457:Echovirus Infections / Q000134:cerebrospinal fluid / Q000453:epidemiology / Q000821:virology*; D004770:Enterovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid / Q000453:epidemiology / Q000821:virology*; D008854:Microscopy, Electron; D009500:Neutralization Tests; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral