Inhibitory effect of Magnolia officinalis and lovastatin on aortic oxidative stress and apoptosis in hyperlipidemic rabbits.
Oxidative stress and apoptosis are 2 major characteristics of the progression of atherosclerosis. Both lovastatin and Magnolia officinalis are hypocholesterolemic agents. Therefore, we investigated the effect of M. officinalis extract on the prevention of atherosclerosis in comparison with lovastatin. Twenty hyperlipidemic rabbits were served one of the following diets: a high-fat and cholesterol diet (cholesterol group, 10% corn oil and 0.5% cholesterol), a high fat and cholesterol diet supplemented with M. officinalis extract (300 mg/kg) or lovastatin (6 mg/kg). The plasma lipids, oxidative stress (measured by free radical, malondialdehyde, and oxidative DNA damage), and arterial lesions significantly decreased in the M. officinalis and lovastatin groups when compared with the cholesterol group. Moreover, the expressions of Fas ligand, caspase 8, and caspase 9 in the aortic arches were also markedly lowered after M. officinalis and lovastatin supplements. Therefore, the results indicate that the antiatherogenic effect of M. officinalis is involved with a suppression of oxidative stress and with the down-regulation of apoptosis-related gene expression in hyperlipidemic rabbits.
Chang
Weng-Cheng
WC
Department of Biological Science and Technology, China Medical University and Hospital, Taichung, Taiwan. winstonwcchang@hotmail.com
Yu
Ya-Mei
YM
Hsu
Yuan-Man
YM
Wu
Chieh-Hsi
CH
Yin
Pao-Lun
PL
Chiang
Su-Yin
SY
Hung
Jui-Sung
JS
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017209:Apoptosis / Q000187:drug effects*; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases / Q000235:genetics; D002784:Cholesterol / Q000097:blood; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D053222:Fas Ligand Protein; D005786:Gene Expression Regulation / Q000187:drug effects; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D006949:Hyperlipidemias / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008148:Lovastatin / Q000494:pharmacology*; D031566:Magnolia; D008315:Malondialdehyde / Q000097:blood; D008562:Membrane Glycoproteins / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D048069:Tumor Necrosis Factors / Q000235:genetics
D053222:Fas Ligand Protein; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008562:Membrane Glycoproteins; D010936:Plant Extracts; D048069:Tumor Necrosis Factors; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002784:Cholesterol; D008148:Lovastatin; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases; D003849:Deoxyguanosine