Inducible and endothelial nitric oxide synthase genes polymorphism in inflammatory bowel disease.
To assess the influence of inducible and endothelial nitric oxide synthase gene (NOS2A and NOS3) polymorphisms in susceptibility to Crohn's disease (CD) and ulcerative colitis (UC). A total of 505 inflammatory bowel disease (IBD) patients (221 with UC and 284 with CD) and 332 ethnically matched controls were studied. Patients and controls were genotyped by polymerase chain reaction -based techniques for a multiallelic (CCTTT)(n) repeat and biallelic marker (TAAA)(n) in the promoter region of the NOS2A gene and for a T/C polymorphism at position -786 in the promoter region and a polymorphism in exon 7(298Glu/Asp) of the NOS3 gene. There was not association between NOS2A and NOS3 genotypes, alleles or haplotypes frequencies with UC, CD and controls. Our data suggest that NOS2A and NOS3 polymorphisms do not play a major role in IBD predisposition.
Oliver
J
J
Instituto de Parasitología y Biomedicina López Neyra, CSIC, Granada, Spain.
Gómez-Garcia
M
M
Vilchez
J R
JR
López-Nevot
M A
MA
Piñero
A
A
Correro
F
F
Nieto
A
A
Martín
J
J
D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000201:enzymology / Q000235:genetics*; D003424:Crohn Disease / Q000201:enzymology / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III