Protein rejecting properties of PEG-grafted nanoparticles: influence of PEG-chain length and surface density evaluated by two-dimensional electrophoresis and bicinchoninic acid (BCA)-proteinassay.
Poly (ethylene glycol) (PEG)-grafted nanoparticles have been described as potential intravenously injectable, long-circulating drug carriers. The in vivo behaviour of intravenous administered nanoparticles is decisively influenced by the interaction of the particles with the blood proteins. Two-dimensional electrophoresis (2-DE) was employed to study the protein rejecting properties of PEG-grafted polymer nanoparticles, possessing PEG-200 and PEG-400 chains, respectively. The calculated PEG-chain distances varied between 0.39/0.31 nm (PEG-200) and 0.39/0.34 nm (PEG-400), therefore it was possible to study the influence of high chain densities attained by the use of short PEG chains on the protein adsorption. Apart from a stronger protein rejection of small-MW proteins achieved by PEG-chain distance diminution, the affinity of several proteins for the PEG-chains are shown and discussed. Beside the study of protein adsorption patterns, the total protein mass adsorbed to the particles, as well as the extent of protein desorption prior to 2-DE, was investigated using the bicinchoninic acid (BCA)-protein assay.
Gessner
A
A
Department of Pharmaceutical Technology, Biotechnology and Quality Management, The Free University of Berlin, Berlin, Germany.
Paulke
B R
BR
Müller
R H
RH
Göppert
T M
TM
D000327:Adsorption; D004337:Drug Carriers / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D007202:Indicators and Reagents; D049329:Nanostructures / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology*; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D011804:Quinolines / Q000737:chemistry*; D013499:Surface Properties
D004337:Drug Carriers; D007202:Indicators and Reagents; D011137:Polystyrenes; D011506:Proteins; D011804:Quinolines; D011092:Polyethylene Glycols; C047117:bicinchoninic acid