title: Simultaneous compensation for spatial and temporal dispersion of acousto-optical deflectors for two-dimensional scanning with a single prism.
abstract: The dispersive nature of the acousto-optical deflector (AOD) presents a challenge to applications of two sequential orthogonal AODs (a two-dimensional AOD) as XY scanners in multiphoton microscopy. Introducing a prism before the two-dimensional (2D) AOD allows both temporal and spatial dispersion to be compensated for simultaneously. A 90 fs laser pulse was broadened to 572 fs without compensation, and 143 fs with compensation. The ratio of long axis to short axis of the exiting laser beam spot was 3.50 without compensation and 1.14 with compensation. The insertion loss was 37%. Two-photon fluorescence microscopy used the compensated 2D AOD scanner to image a fluorescent microsphere, which improves signal intensity -15-fold compared with the uncompensated scanner.
authors.affiliation: National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China. sqzeng@mail.hust.edu.cn
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Lv
authors.forename: Chen
authors.initials: C
authors.lastname: Zhan
authors.forename: Wei R
authors.initials: WR
authors.lastname: Chen
authors.forename: Wenhui
authors.initials: W
authors.lastname: Xiong
authors.forename: Steven L
authors.initials: SL
authors.lastname: Jacques
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
mesh_terms: D016477:Artifacts; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008027:Light; D018613:Microscopy, Confocal / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D012542:Scattering, Radiation