title: Crustacean hyperglycemic and vitellogenesis-inhibiting hormones in the lobster Homarus gammarus.
abstract: Crustacean hyperglycemic hormone (CHH) and vitellogenesis-inhibiting hormone (VIH), produced by the X organ-sinus gland neurosecretory complex, belong to a peptide group referred to as the CHH family, which is widely distributed in arthropods. In this study, genetic variants and post-translationally modified isoforms of CHH and VIH were characterized in the European lobster Homarus gammarus. With the use of RP-HPLC and ELISA with specific antibodies that discriminate between stereoisomers of CHH and VIH, two groups of CHH-immunoreactive peaks were characterized from HPLC fractions of sinus gland extract (CHH A and CHH B); each group contained two variants (CHH and D-Phe3CHH). In the same way, two VIH-immunoreactive peaks (VIH and D-Trp4VIH) were demonstrated in HPLC fractions from sinus gland extract. The masses of these different neuropeptides were determined by FT-ICR MS: CHH A and CHH B spectra exhibited monoisotopic ions at 8557.05 Da and 8527.04 Da, respectively, and both VIH isomers displayed an m/z value of 9129.19 Da. Two full-length cDNAs encoding preprohomones of CHH A and CHH B and only one cDNA for VIH precursor were cloned and sequenced from X organ RNA. Comparison of CHH sequences between European lobster and other Astacoidea suggests that the most hydrophobic form appeared first during crustacean evolution.
authors.affiliation: Université Pierre et Marie Curie-Paris 6, FRE CNRS 2852: Protéines: Biochimie Structurale et Fonctionnelle, Equipe Biogenèse des Peptides Isomères, Paris, France. celine.ollivaux@snv.jussieu.fr
authors.forename: Céline
authors.initials: C
authors.lastname: Ollivaux
authors.forename: Joëlle
authors.initials: J
authors.lastname: Vinh
authors.forename: Daniel
authors.initials: D
authors.lastname: Soyez
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Toullec
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D007447:Invertebrate Hormones / Q000737:chemistry* / Q000235:genetics; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008121:Nephropidae / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D060829:Arthropod Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; C015947:hyperglycemic hormone, crustacean; C073189:vitellogenesis inhibiting hormone