Time and concentration dependence of Fenton-induced oxidation of dG.
The influence of incubation time and Fenton reagent concentrations was investigated on the oxidation of 2'-deoxyguanosine. The compounds identified and quantified, through use of an LC-MS/MS system, were 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'-cyclo-2'-deoxyguanosine (8,5'cyclodG) and the secondary oxidation products guanidinohydantoin and dehydro-guanidinohydantoin. 8-oxodG and 8,5' cyclodG formed very quickly, reaching a maximum rapidly, but with 8-oxodG a rapid decline occurred thereafter due to its further oxidation into the secondary products, which formed more slowly. Due to the better stability, 8,5' cyclodG correlated better with the general level of oxidation than 8-oxodG. The results emphasize the advantages of measuring other oxidation adducts than 8-oxodG alone.
Grey
Carl Elovson
CE
Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Carl.Elovson_Grey@biotek.lu.se
Adlercreutz
Patrick
P
D003849:Deoxyguanosine / Q000737:chemistry / Q000378:metabolism*; D005296:Ferrous Compounds / Q000737:chemistry / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology*; D007501:Iron / Q000737:chemistry / Q000494:pharmacology*; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D013997:Time Factors
C045076:Fenton's reagent; D005296:Ferrous Compounds; C020748:ferrous sulfate; D006861:Hydrogen Peroxide; D007501:Iron; D003849:Deoxyguanosine