Identification of DNA copy-number aberrations by array-comparative genomic hybridization in patients with schizophrenia.
Chromosomal abnormalities are implicated as important markers for the pathogenesis in patients with schizophrenia. In this study, with using bacterial artificial chromosome (BAC) array-based comparative genomic hybridization (CGH), we analyzed DNA copy-number changes among 30 patients with schizophrenia. The most frequent changes were partial gain of Xq23 (52%) and loss of 3q13.12 (32%). Other frequent gains were found in: 1p, 6q, 10p, 11p, 11q, 14p, and 15q regions, and frequent losses were found in: 2p, 9q, 10q, 14q, 20q, and 22q regions. The set of abnormal regions was confirmed by real-time PCR (9q12, 9q34.2, 11p15.4, 14q32.33, 15q15.1, 22q11.21, and Xq23). All real-time PCR results were consistent with the array-CGH results. Therefore, it is suggested that array-CGH and real-time PCR analysis could be used as powerful tools in screening for schizophrenia-related genes. Our results might be useful for further exploration of candidate genomic regions in the pathogenesis of schizophrenia.
Moon
Ho Jin
HJ
Department of Pharmacology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
Yim
Sung-Vin
SV
Lee
Woon Kyu
WK
Jeon
Yang-Whan
YW
Kim
Young Hoon
YH
Ko
Young Jin
YJ
Lee
Kwang-Soo
KS
Lee
Kweon-Haeng
KH
Han
Sang-Ick
SI
Rha
Hyoung Kyun
HK
D000328:Adult; D002869:Chromosome Aberrations / Q000706:statistics & numerical data*; D002874:Chromosome Mapping / Q000379:methods*; D004252:DNA Mutational Analysis / Q000379:methods*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D015994:Incidence; D007723:Korea / Q000453:epidemiology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012559:Schizophrenia / Q000453:epidemiology* / Q000235:genetics*