Isolation, endocrine regulation and mRNA distribution of the 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMG-S) gene from the pine engraver, Ips pini (Coleoptera: Scolytidae).
We isolated a full-length cDNA encoding 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMG-S) from the pine engraver beetle, Ips pini (Say), and examined its genomic structure. The intron-less gene has a predicted 460 amino acid cytosolic protein product with 73% identity to HMG-S from Dendroctonus jeffreyi, and high identity (58-64%) with other insect HMG-Ss. Topically applied juvenile hormone (JH) III induced HMG-S mRNA levels up to 6.5-fold in both sexes, mostly in the anterior midgut, though there were differences between males and females in the timing, sensitivity to JH III dose and tissue distribution of HMG-S mRNA. These data further validate the coordinate regulation of mevalonate pathway genes for de novo isoprenoid pheromone production in bark beetles.
Bearfield
J C
JC
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, 89557, USA.
Keeling
C I
CI
Young
S
S
Blomquist
G J
GJ
Tittiger
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001517:Coleoptera / Q000201:enzymology* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D017344:Genes, Insect; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010675:Pheromones / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012717:Sesquiterpenes / Q000378:metabolism
D010675:Pheromones; D012333:RNA, Messenger; D012717:Sesquiterpenes; C036585:juvenile hormone III; D006904:Hydroxymethylglutaryl-CoA Synthase