title: The effect of VEGF on blood vessels and blood cells during Xenopus development.
abstract: Vascular endothelial growth factor (VEGF) is known to play an essential role in vascular development. We have overexpressed VEGF122 or VEGF170, which are equivalent to mouse VEGF120 and VEGF164, in developing Xenopus embryos. Overexpression of VEGF170 but not VEGF122 demonstrated an absence of expression of hematopoietic markers alpha-globin and GATA-1 but only in the posterior portion of the blood island. Interestingly, strong signals of endothelial markers, msr, fli-1, and tie-2, were detectable in those regions, instead of hematopoietic markers. These results suggested both that injection of VEGF170 resulted in disturbance of vasculogenesis in the posterior portion of the blood island, with excessive production of endothelial cells at the expense of blood cells, and that the anterior and posterior portions of the VBI may have distinct characteristics.
authors.affiliation: Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita 565-0871, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Koibuchi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Taniyama
authors.forename: Kaori
authors.initials: K
authors.lastname: Nagao
authors.forename: Toshio
authors.initials: T
authors.lastname: Ogihara
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Kaneda
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Morishita
mesh_terms: D000818:Animals; D015415:Biomarkers; D001773:Blood Cells / Q000166:cytology; D001808:Blood Vessels / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D019070:Cell Lineage / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000098:blood supply / Q000166:cytology / Q000196:embryology; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology / Q000196:embryology*; D050982:GATA1 Transcription Factor / Q000032:analysis / Q000378:metabolism; D005914:Globins / Q000032:analysis / Q000378:metabolism; D006410:Hematopoiesis / Q000235:genetics; D015533:Transcriptional Activation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000502:physiology*; D029867:Xenopus Proteins / Q000737:chemistry / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D015017:Yolk Sac / Q000098:blood supply / Q000196:embryology
chemical_list: D015415:Biomarkers; D050982:GATA1 Transcription Factor; D042461:Vascular Endothelial Growth Factor A; D029867:Xenopus Proteins; D005914:Globins