Detection of deoxynivalenol in foods and indoor air using an array biosensor.
Deoxynivalenol (DON), a mycotoxin produced by several Fusaruim species, is a worldwide contaminant of foods and feeds. Because of the potential dangers due to accidental or intentional contamination of foods with DON, there is a need to develop a rapid and highly sensitive method for easy identification and quantification of DON. In this study, we have developed and utilized a competitive immunoassay technique to detect DON in various food matrixes and indoor air samples using an array biosensor. A DON-biotin conjugate, immobilized on a NeutrAvidin-coated optical waveguide, competed with the DON in the sample for binding to fluorescently labeled DON monoclonal antibodies. To demonstrate a simple procedure amenable for on-site use, DON-spiked cornmeal, cornflakes, wheat, barley, and oats were extracted with methanol-water (3:1) and assayed without cleanup or preconcentration. The limits of detection ranged from 0.2 ng/mL in buffer to 50 ng/g in oats. The detection limit of DON spiked into an aqueous effluent from an air sampler was 4 ng/mL.
Ngundi
Miriam M
MM
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory,Washington, DC 20375, USA.
Qadri
Syed A
SA
Wallace
Elizabeth V
EV
Moore
Martin H
MH
Lassman
Michael E
ME
Shriver-Lake
Lisa C
LC
Ligler
Frances S
FS
Taitt
Chris R
CR
D016902:Air Pollution, Indoor; D015374:Biosensing Techniques; D005504:Food Analysis; D013050:Spectrometry, Fluorescence; D014255:Trichothecenes / Q000032:analysis*
D014255:Trichothecenes; C007262:deoxynivalenol