title: Cytogenetic and fluorescence in situ hybridization analysis of a basal cell adenocarcinoma of the mandible.
abstract: Basal cell adenocarcinoma (BCAC) of the salivary glands is rare. Distant metastasis to the mandible from a salivary gland tumor is also considered rare. The cytogenetic finding of a case of metastatic BCAC of the mandible is described. We are unaware of earlier reports regarding cytogenetic findings of BCAC either at the primary site or at a distant metastasis site. An 80-year-old female with primary BCAC of the parotid salivary gland underwent parotidectomy and chemotherapy. One year later, a metastatic lesion in the mandible was found. Tissue specimens from the mandibular lesion were tested by the following pathologic methods: hematoxylin-eosin and immunohistochemistry for CK8/18, CK/903, vimentin, and smooth muscle actin. The characteristic histologic architecture of BCAC found in the mandible was similar to that of the earlier findings of the tumor in the parotid gland. A fresh sample from the mandibular lesion was examined by cytogenetics and fluorescence in situ hybridization (FISH), using centromeric probes for chromosomes 4, 8, 10, 18, and 22. A paraffin-embedded sample of the primary tumor was also examined by FISH. Cytogenetic and FISH analyses of the mandibular metastatic lesion revealed a clone with a pericentric inversion of chromosome 17 and a clone with trisomy 4, respectively. Trisomy 4 was also found in the paraffin-embedded samples of the primary parotid tumor.
authors.affiliation: Genetic Institute, Soroka University Medical Center and Ben Gurion University of the Negev, Beer-Sheva, Israel. manore@bgu.ac.il
authors.forename: Esther
authors.initials: E
authors.lastname: Manor
authors.forename: Netta
authors.initials: N
authors.lastname: Sion-Vardy
authors.forename: Lipa
authors.initials: L
authors.lastname: Bodner
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000369:Aged, 80 and over; D007446:Chromosome Inversion / Q000235:genetics; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008339:Mandibular Neoplasms / Q000235:genetics*; D010307:Parotid Neoplasms / Q000235:genetics