Microarray glass slides coated with block copolymer brushes obtained by reversible addition chain-transfer polymerization.
The reversible addition-fragmentation chain-transfer polymerization was used to prepare microarray slides grafted with polymer brushes for DNA-based applications. Block copolymer brushes of N,N-dimethylacrylamide (DMA) and glycidyl methacrylate (GMA), poly(DMA-b-GMA) were prepared by extending living poly(dimethylacrylamide) chains. The functional surface was used as a substrate for oligonucleotide hybridization experiments. The results were compared to those provided by glass slides coated by a self-assembled monolayer made of (3-glycidyloxypropyl)trimethoxysilane. Surfaces coated with block polymer brushes bearing oxirane groups are more efficient as substrates for oligonucleotide hybridization than surfaces coated with nonpolymeric self-assembled monolayers containing the same functional group. The high probe grafting density and hybridization efficiency achieved with this polymeric coating reveal the importance of the block architecture to ensure good accessibility of the immobilized probe. The new surface was characterized by static angle measurements and diffuse reflectance FT-IR spectroscopy on a silica model system.
Pirri
Giovanna
G
Istituto di Chimica del Riconoscimento Molecolare, CNR, Milano, Italy. giovanna.pirri@icrm.cnr.it
Chiari
Marcella
M
Damin
Francesco
F
Meo
Alessandra
A
D000180:Acrylic Resins / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D015394:Molecular Structure; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties
D000180:Acrylic Resins; D009841:Oligonucleotides; D011108:Polymers; C519270:poly(N,N-dimethylacrylamide-b-glycidyl methacrylate); D012822:Silicon Dioxide