title: Increases in COX II mRNA in the rat spinal cord induced by cauda equina traction.
abstract: This article investigated the time response of COX II induction by traction of the cauda equina assessed by a quantified RT-PCR method. Under deep GOI anesthesia, male Wistar rats were fixed in the prone position and a laminectomy of the dorsal part of the first and second sacral vertebrae was performed. Following, COX II-mRNA levels in the cervical, thoracic, lumbar, sacral, and caudal segments were measured at 2, 4, 6, and 24 h after traction by a quantified RT-PCR method. After cauda equina traction, significant levels of COX II mRNA were detected in all segments of the spinal cord examined. Maximum levels in each segment were determined 4 h after traction of the cauda equina. Particularly in the sacrocaudal segments significantly higher levels of COX II mRNA were measured 24 h after traction. These results indicate that significant induction of spinal COX II mRNA was caused by cauda equina traction and that such induction plays a regulatory role in the nociceptive pain pathway.
authors.affiliation: Department of Anesthesiology, Tokyo Medical University, Shinjuku, Tokyo, Japan.
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Hirabayashi
authors.forename: Masashi
authors.initials: M
authors.lastname: Komagata
authors.forename: Jinzo
authors.initials: J
authors.lastname: Yamada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Isshiki
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D009043:Motor Activity / Q000502:physiology; D011128:Polyradiculopathy / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013116:Spinal Cord / Q000378:metabolism* / Q000473:pathology; D018709:Statistics, Nonparametric; D013997:Time Factors; D014143:Traction / Q000379:methods
chemical_list: D012333:RNA, Messenger; D051546:Cyclooxygenase 2