title: Pregnane glycosides from Leptadenia pyrotechnica.
abstract: The whole plant of Leptadenia pyrotechnica afforded 18 new pregnane glycosides (1-18) with sarcostin, 11-hydroxysarcostin, and deacetylmetaplexigenin as the aglycon moieties and acetyl, benzoyl, cinnamoyl, p-coumaroyl, and nicotinoyl ester moieties linked at C-12 and/or C-20 of the aglycon and hexopyranose, 6-deoxy-3-O-methylhexopyranose, and 2,6-dideoxy-3-O-methylhexopyranose sugars linked at C-3 of their aglycon. The structures of these compounds were elucidated by spectroscopic data interpretation and from chemical evidence. The antiproliferative activity of all compounds was evaluated using three continuous murine and human culture cell lines, J774.A1, HEK-293, and WEHI-164. Compounds having deacethylmetaplexigenin as aglycon and a cinnamoyl ester moiety linked at C-12 were the most active constituents.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Salerno, Via Ponte Don Melillo, 84084 Fisciano (SA), Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Cioffi
authors.forename: Rokia
authors.initials: R
authors.lastname: Sanogo
authors.forename: Antonio
authors.initials: A
authors.lastname: Vassallo
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Dal Piaz
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Autore
authors.forename: Stefania
authors.initials: S
authors.lastname: Marzocco
authors.forename: Nunziatina
authors.initials: N
authors.lastname: De Tommasi
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D029062:Apocynaceae / Q000737:chemistry*; D002240:Carbohydrate Sequence; D004354:Drug Screening Assays, Antitumor; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D008302:Mali; D051379:Mice; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D011278:Pregnanes