title: Urinary pseudouridine in patients with lymphoma: comparison with other clinical parameters.
abstract: BACKGROUND
  Some patients with malignant lymphoma do not manifest superficial lymphadenopathy. In such cases, clinical parameters that indicate the number of tumor cells are important for the assessment of tumor growth and choice of proper treatment. We evaluated urinary pseudouridine (U-PU) as an indicator of the growth of malignant lymphoma by comparing its levels with serum concentrations of other clinical parameters in patients with various lymphomas at various stages.
  
  
  METHODS
  Urine was obtained from 67 patients with lymphoma. U-PU was assayed by recombinant Fab-based inhibition ELISA. Serum soluble IL2 receptor (sIL2R), serum deoxythymidine kinase (dTK), serum beta-2 microglobulin (beta2MG) and serum lactate dehydrogenase (LDH) were also assayed.
  
  
  RESULTS
  U-PU concentrations showed good correlations with serum concentrations of beta2MG, LDH, sIL2R and dTK. The level of U-PU was higher in stage IV than in stages I (P=0.023), II (P=0.006) and III (P=0.036).
  
  
  CONCLUSIONS
  U-PU concentration correlates with the clinical stage of lymphoma and is a useful tool to assess the growth of lymphoma.
authors.affiliation: Hematology and Immunology, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Kahoku-gun, Ishikawa 920-0293, Japan. yasum@kanazawa-med.ac.jp <yasum@kanazawa-med.ac.jp>
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Masaki
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Itoh
authors.forename: Toshioki
authors.initials: T
authors.lastname: Sawaki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Karasawa
authors.forename: Takafumi
authors.initials: T
authors.lastname: Kawanami
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Fukushima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawabata
authors.forename: Yuji
authors.initials: Y
authors.lastname: Wano
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hirose
authors.forename: Toshio
authors.initials: T
authors.lastname: Suzuki
authors.forename: Susumu
authors.initials: S
authors.lastname: Sugai
authors.forename: Hisanori
authors.initials: H
authors.lastname: Umehara
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008223:Lymphoma / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011560:Pseudouridine / Q000652:urine*; D015375:Receptors, Interleukin-2 / Q000097:blood; D013937:Thymidine Kinase / Q000097:blood; D001613:beta 2-Microglobulin / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor; D015375:Receptors, Interleukin-2; D001613:beta 2-Microglobulin; D011560:Pseudouridine; D007770:L-Lactate Dehydrogenase; D013937:Thymidine Kinase