Methanogenesis is Ca2+ dependent in Methanothermobacter thermautotrophicus strain DeltaH.
The effect of Ca2+ ions on methanogenesis and growth of Methanothermobacter thermautotrophicus was investigated. The calcium chelator ethylene glycol bis(2-aminoethylether)-N,N,N',N'-tetra-acetic acid, calcium ionophore A23187 and ruthenium red all inhibited growth of this strain. Methane formation was strongly dependent on the external Ca2+ concentration in a resting cell suspension. In addition, methanogenesis of Ca2+ preloaded cells was stimulated by 400%. Inhibitor studies revealed that Co2+ and Ni2+, inorganic antagonists of Ca2+ transport, strongly inhibited methanogenesis in these cells. Interestingly, our findings imply that one of the enzymes of methanogenesis might catalyse a Ca2+ -dependent step and allow a direct activation of methanogenesis by Ca2+ ions.
Vancek
Matús
M
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic.
Vidová
Monika
M
Majerník
Alan I
AI
Smigán
Peter
P
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D001692:Biological Transport / Q000502:physiology; D002118:Calcium / Q000037:antagonists & inhibitors / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D003470:Culture Media; D004533:Egtazic Acid / Q000494:pharmacology; D008697:Methane / Q000096:biosynthesis*; D017013:Methanobacteriaceae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012430:Ruthenium Red / Q000494:pharmacology
D002121:Calcium Channel Blockers; D003470:Culture Media; D012430:Ruthenium Red; D004533:Egtazic Acid; D000255:Adenosine Triphosphate; D008697:Methane; D002118:Calcium