[Establishment of immortalized B lymphoblast cell line from patients with chronic hepatitis B].
OBJECTIVE
To establish immortalized B lymphoblast cell line (BLCL) from patients with chronic hepatitis B (CHB) in vitro.


METHODS
The peripheral blood mononuclear cells (PBMC) were isolated from the patients with CHB by routine method and incubated with EB virus (EBV) in the presence of CpG DNA motifs and cyclosporin A (CysA) for about 28 days. Morphological characteristic of the established immortalized BLCL was observed by microscope and the expression of CD19 and CD23 on cellular surface was determined by flow cytometry.


RESULTS
BLCL was successfully established and could be cultured and propagated for a long time in vitro.


CONCLUSIONS
EBV-immortalized BLCL provides a resource of target cells for further research on the cellular immunity of patients with CHB.
Zhang
Ming-xia
MX
Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. zhangmx@fimmu.com
Diao
Zhi-hong
ZH
He
Hai-tang
HT
Hou
Jin-lin
JL
Luo
Kang-xian
KX
D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000473:pathology* / Q000821:virology; D018929:Cell Culture Techniques; D002460:Cell Line; D002478:Cells, Cultured; D006509:Hepatitis B / Q000473:pathology; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000737:chemistry; D019694:Hepatitis B, Chronic / Q000473:pathology*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D015703:Antigens, CD; C439765:C19orf33 protein, human; D006513:Hepatitis B e Antigens; D009687:Nuclear Proteins; D014157:Transcription Factors