Minor components of olive oil modulate proatherogenic adhesion molecules involved in endothelial activation.
The Mediterranean diet reduces the risk of coronary artery disease as a consequence of its high content of antioxidants, namely, hydroxytyrosol (HT) and oleuropein aglycone (OleA), typical of virgin olive oil. Because intercellular and vascular cell adhesion molecules (ICAM-1 and VCAM-1) and E-selectin are crucial for endothelial activation, the role of the phenolic extract from extra virgin olive oil (OPE), OleA, HT, and homovanillyl alcohol (HVA) on cell surface and mRNA expression in human umbilical vascular endothelial cells (HUVEC) was evaluated. OPE strongly reduced cell surface expression of ICAM-1 and VCAM-1 at concentrations physiologically relevant (IC50 < 1 microM), linked to a reduction in mRNA levels. OleA and HT were the main components responsible for these effects. HVA inhibited cell surface expression of all the adhesion molecules, whereas the effect on mRNA expression was weaker. These results supply new insights on the protective role of olive oil against vascular risk through the down-regulation of adhesion molecules involved in early atherogenesis.
Dell'Agli
Mario
M
Department of Pharmacological Sciences, University of Milan, Italy. mario.dellagli@unimi.it
Fagnani
Rossana
R
Mitro
Nico
N
Scurati
Samuele
S
Masciadri
Maura
M
Mussoni
Luciana
L
Galli
Germana V
GV
Bosisio
Enrica
E
Crestani
Maurizio
M
De Fabiani
Emma
E
Tremoli
Elena
E
Caruso
Donatella
D
D015815:Cell Adhesion Molecules / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D019040:E-Selectin / Q000032:analysis / Q000235:genetics; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000235:genetics; D057888:Iridoid Glucosides; D039823:Iridoids; D000069463:Olive Oil; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology*; D011714:Pyrans / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D014471:Umbilical Veins / Q000737:chemistry; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000235:genetics
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D057888:Iridoid Glucosides; D039823:Iridoids; D000069463:Olive Oil; D010938:Plant Oils; D011714:Pyrans; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; C005975:3,4-dihydroxyphenylethanol; D018799:Intercellular Adhesion Molecule-1; C002769:oleuropein; D010626:Phenylethyl Alcohol