Measurement of dietary choline-phospholipid content by a novel phospholipase D-triiodide method.
We developed a novel method that conveniently measures dietary choline-phospholipid content. Crude lipids extracted from dietary samples were reacted with phospholipase D from Streptomyces chromofuscus. The choline liberated from this reaction was then reacted with potassium triiodide, yielding choline periodide, which could be measured spectrophotometrically at 365 nm. This method proved to be more convenient than conventional assays, such as thin layer chromatography and high performance liquid chromatography. Our novel method is suitable for measuring many samples in single experiments.
Ueda
Aiko
A
Department of Health Sciences, Prefectural University of Hiroshima, Japan.
Hanada
Ai
A
Ishinaga
Masataka
M
D002794:Choline / Q000032:analysis*; D004032:Diet; D005504:Food Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007454:Iodides / Q000737:chemistry*; D016014:Linear Models; D010713:Phosphatidylcholines / Q000737:chemistry; D010739:Phospholipase D / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis*; D013053:Spectrophotometry; D013302:Streptomyces / Q000201:enzymology
D007454:Iodides; D010713:Phosphatidylcholines; D010743:Phospholipids; D010739:Phospholipase D; D002794:Choline; C010389:Lugol's solution