Quantitative determination of jatrophone in "cachaça" prepared with Jatropha elliptica.
A method for sample preparation and analysis by high performance liquid chromatography with UV detection (HPLC-UV) was developed for analysis of jatrophone in "cachaça" prepared with Jatropha elliptica, administered orally, employed in Brazil for the treatment of venomous snake bites. The linearity, accuracy, precision of the procedure was evaluated. Analytical curve for jatrophone was linear in the range of 16.24-81.20 microg ml(-1). The recovery of the jatrophone in the samples analyzed was 98.99-99.89%. The percent coefficient of variation for the quantitative analysis of the "cachaça" in the analyses was under 2%.
Brum
Rosenei Louzada
RL
Universidade Federal de Mato Grosso do Sul, Departamento de Química, Caixa Postal 649, 79070-900 Campo Grande/MS, Brazil.
Cardoso
Claudia Andréa Lima
CA
Honda
Neli Kika
NK
Andreu
Maxwell Parrela
MP
Viana
Luiz Leonardo de Souza
LL
D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis*; D001938:Brazil; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000032:analysis*; D004355:Drug Stability; D031286:Jatropha / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C006386:jatrophone