Influence of xanthohumol on the binding behavior of GABAA receptors and their lateral mobility at hippocampal neurons.
The influence of the xanthohumol from Humulus lupulus L. on the binding of muscimol-Alexa Fluor 532 (Mu-Alexa), a fluorescently labeled GABAA receptor agonist, was studied by fluorescence correlation spectroscopy. An incubation of hippocampal neurons with 75 nM of xanthohumol increased the specific Mu-Alexa binding by approximately 17%, which was selectively found in GABAA receptor Mu-Alexa complexes with hindered lateral mobility [D(bound2) = (0.11 +/- 0.03) microm2/s] as described with midazolam.
Meissner
Oliver
O
Department of Pharmaceutical Biology, University of Marburg, Marburg, Germany.
Häberlein
Hanns
H
D000818:Animals; D005419:Flavonoids; D006624:Hippocampus / Q000166:cytology*; D027582:Humulus / Q000737:chemistry; D066298:In Vitro Techniques; D009118:Muscimol; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011427:Propiophenones / Q000494:pharmacology*; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D005419:Flavonoids; D011427:Propiophenones; D011963:Receptors, GABA-A; D009118:Muscimol; C104536:xanthohumol