Nitric oxide contributes to the development of a post-injury Th2 T-cell phenotype and immune dysfunction.
Severe injury induces immune dysfunction resulting in increased susceptibility to opportunistic infections. Previous studies from our laboratory have demonstrated that post-burn immunosuppression is mediated by nitric oxide (NO) due to the increased expression of macrophage inducible nitric oxide synthase (iNOS). In contrast, others suggest that injury causes a phenotypic imbalance in the regulation of Th1- and Th2 immune responses. It is unclear whether or not these apparently divergent mediators of immunosuppression are interrelated. To study this, C57BL/6 mice were subjected to major burn injury and splenocytes were isolated 7 days later and stimulated with antiCD3. Burn injury induced NO-mediated suppression of proliferative responses that was reversed in the presence of the NOS inhibitor L-monomethyl-L-arginine and subsequently mimicked by the addition of the NO donor, S-nitroso-N-acetyl-penicillamine (SNAP). SNAP also dose-dependently suppressed IFN-gamma and IL-2 (Th1), but not IL-4 and IL-10 (Th2) production. Delaying the addition of SNAP to the cultures by 24 h prevented the suppression of IFN-gamma production. The Th2 shift in immune phenotype was independent of cGMP and apoptosis. The addition of SNAP to cell cultures also induced apoptosis, attenuated mitochondrial oxidative metabolism and induced mitochondrial membrane depolarization. However, these detrimental cellular effects of NO were observed only at supra-physiologic concentrations (>250 microM). In conclusion, these findings support the concept that NO induces suppression of cell-mediated immune responses by selective action on Th1 T cells, thereby promoting a Th2 response.
Daniel
Tanjanika
T
Department of Surgery, Center for Surgical Research, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
Alexander
Michelle
M
Hubbard
William J
WJ
Chaudry
Irshad H
IH
Choudhry
Mashkoor A
MA
Schwacha
Martin G
MG
D000818:Animals; D002056:Burns; D017252:CD3 Complex / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005260:Female; D007154:Immune System Diseases / Q000209:etiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009566:Nitrates / Q000032:analysis / Q000378:metabolism; D009569:Nitric Oxide / Q000494:pharmacology*; D009573:Nitrites / Q000032:analysis / Q000378:metabolism; D010641:Phenotype; D026423:S-Nitroso-N-Acetylpenicillamine / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology*
D017252:CD3 Complex; D016207:Cytokines; D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine