Occurrence and physico-serological properties of potato virus S (PVS) in Iran.
1818 collected samples of potato plant showing virus infection symptoms from 85 fields were tested for PVS infection using DAS-ELISA. Average of infection to this virus varied from 0 to 100%. Least infection was belonging to fields with new introduced varieties. On the other hand native and old introduced cultivars showed heavy infection. In field condition, PVS infected plants didn't show very obvious symptoms, so some infected plants may be missed in field sample collecting. The physical properties of 3 isolates, Avaj, Stanboly and Agria No 15 were determined. TIP 55-60 degrees C, DEP 10(-3) and Liv measured 3-4 days. Ouchterlony agar double diffusion test using SDS was useful for virus detection and precipitation lines didn't show any spur between isolates, although isolates differs slightly in symptomatology. SDS-Page and Western blotting methods used successfully for virus detection and determining and measuring viral protein components.
Mosahebi
G
G
Agriculture College, University of Tehran.
Ahoon-Manesh
A
A
Sharifi-Tehrani
A
A
Mohammadi
M
M
Okhovvat
S M
SM
D017788:Carlavirus / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D015994:Incidence; D007492:Iran / Q000453:epidemiology; D010935:Plant Diseases / Q000821:virology*; D011198:Solanum tuberosum / Q000821:virology*