Effect of L-ascorbic acid on nickel-induced alterations in serum lipid profiles and liver histopathology in rats.
Nickel exposure greatly depletes intracellular ascorbate and alters ascorbate-cholesterol metabolism. We studied the effect of the simultaneous oral treatment with L-ascorbic acid (50 mg/100 g body weight (BW) and nickel sulfate (2.0 mg/100 g BW, i.p) on nickelinduced changes in serum lipid profiles and liver histopathology. Nickel-treated rats showed a significant increase in serum low-density lipoprotein-cholesterol, total cholesterol, triglycerides, and a significant decrease in serum high-density lipoprotein-cholesterol. In the liver, nickel sulfate caused a loss of normal architecture, fatty changes, extensive vacuolization in hepatocytes, eccentric nuclei, and Kupffer cell hypertrophy. Simultaneous administration of L-ascorbic acid with nickel sulfate improved both the lipid profile and liver impairments when compared with rats receiving nickel sulfate only. The results indicate that L-ascorbic acid is beneficial in preventing nickel-induced lipid alterations and hepatocellular damage.
Das
Kusal K
KK
Department of Physiology, Al-Ameen Medical College, Bijapur 586108 Karnataka, India. kusaldas@yahoo.com
Gupta
Amrita Das
AD
Dhundasi
Salim A
SA
Patil
Ashok M
AM
Das
Swastika N
SN
Ambekar
Jeevan G
JG
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000097:blood*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009532:Nickel / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D014815:Vitamins / Q000494:pharmacology
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D008055:Lipids; D014280:Triglycerides; D014815:Vitamins; D009532:Nickel; D002784:Cholesterol; D001205:Ascorbic Acid