title: SEL1L expression in non-small cell lung cancer.
abstract: SEL1L gene product plays a role in cell transformation and tumor progression in human breast, pancreas, esophageal, and prostate cancer. SEL1L expression was evaluated in a series of 76 surgically resected non-small cell lung carcinomas to investigate its clinical significance. SEL1L is scarcely detectable in normal lung, whereas in the initial stages of cell transformation, it becomes consistently expressed with evident staining in bronchial squamous metaplasia and in associated dysplastic changes. SEL1L immunoreactivity can be detected both in the cytoplasm and less commonly in the nuclei; the subcellular location correlates with tumor histotype, with cytoplasmic immunoreactivity being most prevalent in squamous cell carcinomas (P = .0005) and nuclear immunoreactivity being associated with adenocarcinomas (P = .02). Nuclear import and export signals are present in the SEL1L coding sequence, justifying the different subcellular location of the protein. SEL1L immunoreactivity was inversely correlated with tumor grade (P = .05); when considering only the adenocarcinomas, a stronger association was found (P = .006). SEL1L messenger RNA and protein evaluation in lung cancer cell lines confirmed the expression of the gene and the dual subcellular location of the protein in lung tumors. The data here reported suggest that, in non-small cell lung carcinoma, SEL1L may be an indicator of cell transformation, thus having important biologic and clinical implications.
authors.affiliation: Division of Pathology, Department of Medicine, Surgery and Dental Sciences, University of Milan, A.O. S. Paolo and Fondazione Ospedale Maggiore Policlinico, Mangiagalli, Regina Elena, 20142 Milan, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Ferrero
authors.forename: Monica
authors.initials: M
authors.lastname: Falleni
authors.forename: Monica
authors.initials: M
authors.lastname: Cattaneo
authors.forename: Giulia
authors.initials: G
authors.lastname: Malferrari
authors.forename: Cristina
authors.initials: C
authors.lastname: Canton
authors.forename: Laura
authors.initials: L
authors.lastname: Biagiotti
authors.forename: Marco
authors.initials: M
authors.lastname: Maggioni
authors.forename: Mario
authors.initials: M
authors.lastname: Nosotti
authors.forename: Guido
authors.initials: G
authors.lastname: Coggi
authors.forename: Silvano
authors.initials: S
authors.lastname: Bosari
authors.forename: Ida
authors.initials: I
authors.lastname: Biunno
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004252:DNA Mutational Analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008168:Lung / Q000033:anatomy & histology / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C117384:SEL1L protein, human