title: Biogenesis of the bacterial respiratory CuA, Cu-S enzyme nitrous oxide reductase.
abstract: Nitrous oxide reductase (NosZ, EC 1.7.99.6) is the terminal oxidoreductase of a respiratory electron transfer chain that transforms nitrous oxide to dinitrogen. The enzyme carries six Cu atoms. Two are arranged in the mixed-valent binuclear CuA site, and four make up the mu4-sulfide-bridged Cu cluster, CuZ. The biogenesis of a catalytically active NosZ requires auxiliary functions for metal center assembly in the periplasm. Both Tat and Sec pathways share the task to transport the various Nos proteins to their functional sites. Biogenesis of NosZ requires an ABC transporter complex and the periplasmic Cu chaperone NosL. Sustaining whole-cell NosZ function depends on the periplasmic, FAD-containing protein NosX, and the membrane-bound iron-sulfur flavoprotein NosR. Most components with a biogenetic function are now amenable to structural studies.
authors.affiliation: Institute of Applied Biosciences, Division of Molecular Microbiology, University of Karlsruhe, Karlsruhe, Germany. wgz@mbio.uka.de
authors.forename: Walter G
authors.initials: WG
authors.lastname: Zumft
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D020134:Catalytic Domain / Q000502:physiology; D003300:Copper / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D008074:Lipoproteins / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism*; D021122:Protein Subunits
chemical_list: D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008074:Lipoproteins; D021122:Protein Subunits; D003300:Copper; D010088:Oxidoreductases; C022763:nitrous oxide reductase