Fermentation of 12% (w/v) glucose to 1.2 M lactate by Escherichia coli strain SZ194 using mineral salts medium.
A non-recombinant mutant of Escherichia coli B, strain SZ194, was developed that produces over 1 M D-lactate from glucose (or sucrose) in 72 h using mineral salts medium supplemented with 1 mM: betaine in simple anaerobic fermentations. Rates and yields were highest at pH 7.5. Yields approached the theoretical maximum with only trace amounts of co-products. Chiral purity of D-lactate was estimated to be 95%. Specific and volumetric productivities for SZ194 in mineral salts medium (pH 7.5) with betaine were equivalent to those in Luria broth.
Zhou
S
S
Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.
Shanmugam
K T
KT
Yomano
L P
LP
Grabar
T B
TB
Ingram
L O
LO
D001483:Base Sequence; D001709:Biotechnology; D003470:Culture Media; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D019020:Directed Molecular Evolution; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism*; D008903:Minerals; D012492:Salts; D013045:Species Specificity
D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial; D008903:Minerals; D012492:Salts; D019344:Lactic Acid; D005947:Glucose