title: SYBR green and TaqMan real-time PCR assays are equivalent for the diagnosis of dengue virus type 3 infections.
abstract: Dengue is the most important arthropod-borne viral disease in the world. A rapid diagnostic test for dengue is warranted, and real-time polymerase chain reaction may improve diagnosis. TaqMan and Sybr Green systems were evaluated for the diagnosis of dengue virus type 3 (DENV-3) infections. Out of 77 patients with clinical suspicion of dengue infection, specific IgM antibodies were detected in 40 patients. DENV-3 was detected and quantitated in 17 IgM-positive samples by both systems. These assays were shown to be rapid, and specific for detection of DENV-3, and that for diagnostic purposes, there is no difference between these two assays.
authors.affiliation: Department of Internal Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Alessandra Cristina
authors.initials: AC
authors.lastname: Gomes-Ruiz
authors.forename: Renata Teodoro
authors.initials: RT
authors.lastname: Nascimento
authors.forename: Sérgio Oliveira
authors.initials: SO
authors.lastname: de Paula
authors.forename: Benedito Antônio
authors.initials: BA
authors.lastname: Lopes da Fonseca
mesh_terms: D003715:Dengue / Q000175:diagnosis* / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis; D010948:Viral Plaque Assay
chemical_list: D012367:RNA, Viral