Classification of human liver transplant recipients by their preoperative CD8+ T cell subpopulation and its relation to outcome.
The primed status of T cells is markedly different among liver transplant recipients, due to a lifetime of antigen exposure and reduced thymopoiesis by aging, and diseases. This study aims to characterize the preoperative immunological status of CD8+ T cell subpopulations and relate it to the outcome for liver transplant recipients. We classified 112 liver transplant recipients into 5 groups, based on hierarchical clustering of the CD8+CD45 isoform proportion of T cells. In Groups I and II (pediatric), the naive T cell proportion was more than 50%. In adult recipients, Group III was characterized by a naive T cell proportion of 50%, Group IV had the greatest effector/memory T cells (EM), and Group V had the greatest proportion of effector T cells. In Groups IV and V, the effector T cell proportion was considerably higher, and was accompanied by marked downregulation of the CD27+CD28+ subsets and upregulation of interferon gamma (IFN)-gamma, tumor necrosis factor-alpha, and perforin expression. Group V recipients tended to be complicated postoperatively, with a significantly reduced survival rate (1 yr, 66.8%) and markedly reduced Eastern Cooperative Oncology Group performance status.
Tanaka
Koichi
K
Department of Transplantation and Immunology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Ozawa
Kazue
K
Teramukai
Satoshi
S
Takada
Yasutsugu
Y
Egawa
Hiroto
H
Kaihara
Satoshi
S
Fujimoto
Yasuhiro
Y
Ogura
Yasuhiro
Y
Kasahara
Mureo
M
Ono
Masako
M
Sato
Hiroshi
H
Takai
Kenji
K
Fukushima
Masanori
M
Minato
Nagahiro
N
D000293:Adolescent; D000328:Adult; D000368:Aged; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000096:biosynthesis; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000032:analysis; D016031:Liver Transplantation / Q000009:adverse effects / Q000145:classification / Q000276:immunology*; D019520:Living Donors; D008875:Middle Aged; D011183:Postoperative Complications / Q000209:etiology
D016207:Cytokines; D017493:Leukocyte Common Antigens