[Relationship between CD105 and angiogenesis and biological behaviors in squamous carcinoma of larynx].
OBJECTIVE
To investigate the expression of CD105 in angiogenesis of laryngeal squamous carcinoma and in its biological behaviors significance.


METHODS
To use double staining of immunohistochemistry for CD34/Ki67 and CD105/Ki67 in 30 laryngeal carcinomas and 10 normal mucosae respectively. Anti CD34 Mb and anti CD105 Mb were used to count microvessel density (MVD), respectively. Anti Ki67 Mb marked proliferous endothelium cell. The results were analyzed in relation to the clinical data.


RESULTS
It revealed that higher levels of MVDCD105 in tumor tissue was observed than in normal samples(P<0.05). Proliferation index (PI) marked by CD105 was higher than that marked by CD34(P<0.01). A significant difference between high grade of differentiation and low grade of differentiation was found in MVDCD34 or MVDCD105 (P<0.05, both). There was no significant difference between lymph node metastasis and MVDCD34 or MVDCD105 (P>0.05). MVDCD105 increased along with increased tumor aggressiveness(P<0.01) and elevated clinical stages (P<0.01), but there was no correlation between MVDCD34 and tumor aggressiveness, clinical stages(P>0.05).


CONCLUSIONS
CD105 is a differential marker of newly angiogenic blood vessel. The newly angiogenic blood vessel density was associated with tumor size and clinical stages, but not with lymph node metastasis.
Gu
Xin
X
Department of Otolaryngology-Head and Neck Surgery, Harbin Medical University Affiliated the Second Hospital, China. gn1xin1@yahoo.com.cn
Xu
Yangyang
Y
Wu
He
H
Xu
Xiuyu
X
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D000071063:Endoglin; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D055806:Microvessels; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D011956:Receptors, Cell Surface / Q000378:metabolism*
D015703:Antigens, CD; D018952:Antigens, CD34; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface