A micromethod for the quantification of atenolol in plasma using high-performance liquid chromatography with fluorescence detection: therapeutic drug monitoring of two patients with severe coronary insufficiency before cardiac surgery.
A simple, rapid, selective, and sensitive analytical method was developed for the quantification of atenolol in small volumes of plasma, by high-performance liquid chromatography with fluorescence detection. Only 200 microL of plasma was used for chromatographic analysis. Separation was performed on a C18 reverse-phase column (4 microm) using a binary mobile phase consisting of 0.05 M of phosphate buffer, pH 5.5, and methanol (80:20, vol/vol) at a flow rate of 0.7 mL/minute. The retention times of atenolol and of the internal standard (sotalol) were 12.7 and 10.4 minutes, respectively. Validation of this analytical method showed a good linear correlation (8-2000 ng/mL), high sensitivity (quantification limit: 8 ng/ml and detection limit: 4 ng/mL), accuracy of 99.3%, and intraday and interday precision of 5.3% and 6.9%, respectively. Absolute recovery was 93.7%. The method was found to be robust, with acceptable stability. The analytical method was validated by the quantification of atenolol in plasma obtained from 2 patients with unstable angina, scheduled for myocardium revascularization surgery, who were chronically treated with 50 mg of atenolol administered per os once a day. The method developed was found to be adequate for use in pharmacokinetic studies and in adjusted dose pharmacotherapy.
Leite
Fátima da Silva
Fda S
School of Pharmaceutical Sciences University of Sao Paulo, São Paulo, Brazil.
Pereira
Valéria Adriana
VA
Omosako
Célia Etsuco
CE
Carmona
Maria José Carvalho
MJ
Auler
José Otávio Costa
JO
Santos
Silvia Regina Cavani Jorge
SR
D000284:Administration, Oral; D000319:Adrenergic beta-Antagonists / Q000097:blood / Q000627:therapeutic use; D000369:Aged, 80 and over; D000789:Angina, Unstable / Q000097:blood / Q000188:drug therapy / Q000601:surgery; D001262:Atenolol / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D004355:Drug Stability; D005453:Fluorescence; D006801:Humans; D008832:Microchemistry / Q000191:economics / Q000379:methods*; D008875:Middle Aged; D015203:Reproducibility of Results; D013015:Sotalol / Q000097:blood / Q000493:pharmacokinetics / Q000627:therapeutic use; D013696:Temperature; D013997:Time Factors
D000319:Adrenergic beta-Antagonists; D001262:Atenolol; D013015:Sotalol