title: Ultrastructure of intraradicular dentin after irrigation with BioPure MTAD. II. The consequence of obturation with an epoxy resin-based sealer.
abstract: In addition to an initial report on the creation of thick demineralized collagen matrices in root dentin that were irrigated with BioPure MTAD or EDTA as final rinses for 5 minutes, this study examined the effect of a 2-minute irrigation time of these final irrigants on the extent of demineralization of intact intraradicular dentin and on interfacial nanoleakage after obturation with AH Plus and gutta-percha using warm vertical compaction. Transmission electron microscopy confirmed that the reduction in irrigation time did not compromise the smear layer removing capacity of these two irrigants when 1.3% NaOCl was employed as the initial rinse. BioPure MTAD created 5 to 6 microm thick demineralized dentin matrices, while 17% EDTA produced 1 to 2 microm thick matrices that were suboptimally infiltrated by the epoxy resin-based sealer, as manifested by the occurrence of silver tracer deposits within the hybrid layers.
authors.affiliation: Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, 30912, USA.
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Tay
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Hosoya
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Loushine
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: R Norman
authors.initials: RN
authors.lastname: Weller
authors.forename: Danny C Y
authors.initials: DC
authors.lastname: Low
mesh_terms: D002614:Chelating Agents / Q000009:adverse effects; D019343:Citric Acid / Q000009:adverse effects; D003763:Dental Leakage / Q000139:chemically induced; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure*; D003806:Dentin Permeability / Q000187:drug effects*; D004318:Doxycycline / Q000009:adverse effects; D004492:Edetic Acid / Q000009:adverse effects; D004853:Epoxy Resins; D006801:Humans; D046529:Microscopy, Electron, Transmission; D011136:Polysorbates / Q000009:adverse effects; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants / Q000009:adverse effects*; D012389:Root Canal Obturation / Q000379:methods; D016369:Smear Layer; D017001:Tooth Demineralization / Q000139:chemically induced*
chemical_list: D002614:Chelating Agents; D004853:Epoxy Resins; C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; C534916:epoxy resin-based root canal sealer; D019343:Citric Acid; D004492:Edetic Acid; D004318:Doxycycline