title: Lactobacilli binding human A-antigen expressed in intestinal mucosa.
abstract: Adherent lactic acid bacteria (LAB) in the human intestine were investigated using the surface plasmon resonance technique with the biosensor BIACORE-1000. Ninety-three LAB strains were isolated from human feces and evaluated for binding to human blood type-A antigen [GalNAcalpha1-3 (Fucalpha1-2) Gal-: A-trisaccharide] expressed in the intestinal mucosa. Eleven strains showed strong adherence to an A-trisaccharide biotinyl polymer (BP) probe, and slightly or no adherence to a B-trisaccharide BP probe. Four strains with high adherence (high A/B ratio) were selected and their surface layer proteins (SLPs) were evaluated for A-antigen ligand binding using BIACORE. The SLP from L. brevis strain OLL2772 showed a single band at ca. 48 kDa by SDS-PAGE analysis and it had a very strong adherence to the human A-antigen, as shown using an anti-A lectin blocking technique. A partial N-terminal sequence of the band showed strong homology to an S-layer protein of L. brevis ATCC8287T. The probiotic LAB binds to human blood type-A antigen expressed in the intestinal mucosa which may aid in colonization of the gut.
authors.affiliation: Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Miyagi, Japan. hideaki7@bios.tohoku.ac.jp
authors.forename: Hideaki
authors.initials: H
authors.lastname: Uchida
authors.forename: Hideki
authors.initials: H
authors.lastname: Kinoshita
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawai
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
authors.forename: Koh
authors.initials: K
authors.lastname: Miura
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shiiba
authors.forename: Akira
authors.initials: A
authors.lastname: Horii
authors.forename: Katsunori
authors.initials: K
authors.lastname: Kimura
authors.forename: Naoki
authors.initials: N
authors.lastname: Taketomo
authors.forename: Munehiro
authors.initials: M
authors.lastname: Oda
authors.forename: Takaji
authors.initials: T
authors.lastname: Yajima
authors.forename: Tadao
authors.initials: T
authors.lastname: Saito
mesh_terms: D000017:ABO Blood-Group System / Q000378:metabolism*; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism; D015374:Biosensing Techniques; D005243:Feces / Q000382:microbiology; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: D000017:ABO Blood-Group System; D001426:Bacterial Proteins; C490390:SlpB protein, Lactobacillus brevis