title: Molecular characterization and clinical evaluation of dengue outbreak in 2002 in Bangladesh.
abstract: During the febrile illness epidemic in Bangladesh in 2002, 58 people died out of the 6,132 affected. Two hundred hospitalized patients were analyzed clinically, serologically and virologically to determine the features of this dengue infection. Among the 10- to 70-year-old age group of the 200 clinically suspected dengue patients, 100 (50%) were confirmed as dengue cases by virus isolation and dengue IgM-capture ELISA. Of the 100 dengue-confirmed cases, the mean age was 29.0 (+/-12.4). The possible dengue secondary infection rate determined by Flavivirus IgG-indirect ELISA was 78% in 2002. Eight dengue virus strains were isolated, representing the first dengue virus isolation in the country, and all of the strains were dengue virus type-3 (DEN-3). Sequence data for the envelope gene of the DEN-3 Bangladeshi isolates were used in a phylogenetic comparison with DEN-3 from other countries. A phylogenetic analysis revealed that all 8 strains of DEN-3 were clustered within a well-supported independent sub-cluster of genotype II and were closely related to the Thai isolates from the 1990s. Therefore, it is likely that the currently circulating DEN-3 viruses entered Bangladesh from neighboring countries.
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.
authors.forename: Mohammed Alimul
authors.initials: MA
authors.lastname: Islam
authors.forename: Muzahed Uddin
authors.initials: MU
authors.lastname: Ahmed
authors.forename: Nasima
authors.initials: N
authors.lastname: Begum
authors.forename: Naseem Akhtar
authors.initials: NA
authors.lastname: Chowdhury
authors.forename: Afjal Hossain
authors.initials: AH
authors.lastname: Khan
authors.forename: Maria del Carmen
authors.initials: Mdel C
authors.lastname: Parquet
authors.forename: Sophie
authors.initials: S
authors.lastname: Bipolo
authors.forename: Shingo
authors.initials: S
authors.lastname: Inoue
authors.forename: Futoshi
authors.initials: F
authors.lastname: Hasebe
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Kouichi
authors.initials: K
authors.lastname: Morita
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D001459:Bangladesh / Q000453:epidemiology; D001483:Base Sequence; D002648:Child; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M