Influence of dimethyl sulfoxide on extracellular enzyme production by Pleurotus ostreatus.
Dimethyl sulfoxide (DMSO) is commonly used as a co-solvent to dissolve poorly water-soluble biologically active agents to assess their biological activities such as for enzyme induction. The question addressed was whether DMSO can be assumed to be an inert co-solvent. The influence of DMSO on the production of extracellular enzymes by Pleurotus ostreatus was investigated. DMSO functioned as either an inducer or a repressor, depending on the enzyme studied. The production of laccase and endo-1,4-beta-xylanase increased by 29 and 250%, respectively, in presence of DMSO. However, DMSO repressed the activities of manganese peroxidase, beta-glucosidase, beta-xylanase, and endo-1,4-beta-glucanase by 30, 33, 99 and 16%, respectively. These results raise concerns about the interpretation of bioactivity measurements when DMSO is assumed to function as an inert co-solvent to solubilize water-insoluble molecules.
Shah
Vishal
V
Department of Biology, Dowling College, NY 11769, USA.
Baldrian
Petr
P
Eichlerova
Ivana
I
Dave
Rachna
R
Madamwar
Datta
D
Nerud
Frantisek
F
Gross
Richard
R
D001709:Biotechnology; D002480:Cellulase / Q000096:biosynthesis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D042845:Laccase / Q000096:biosynthesis; D010544:Peroxidases / Q000096:biosynthesis; D020076:Pleurotus / Q000187:drug effects* / Q000201:enzymology*; D012995:Solubility; D012997:Solvents / Q000494:pharmacology; D014867:Water; D014995:Xylosidases / Q000096:biosynthesis; D001617:beta-Glucosidase / Q000096:biosynthesis
D004791:Enzyme Inhibitors; D012997:Solvents; D014867:Water; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; D014995:Xylosidases; D001617:beta-Glucosidase; C026579:exo-1,4-beta-D-xylosidase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D004121:Dimethyl Sulfoxide