title: DMRT-1 expression during NEC8 human embryonic carcinoma cell differentiation.
abstract: To elucidate the relationship between dsx and mab-3 related transcription factor 1 (Dmrt-1) and differentiation, alteration in mRNA levels during differentiation of NEC8 human embryonic carcinoma cells was investigated. After stimulation with 50 nM phorbol 12-myristate 13-acetate (PMA), the cells differentiated into cells with mesenchymal characteristics and upregulated Dmrt-1 mRNA, possibly through the protein kinase C/mitogen-activated protein kinase/activated protein-1 signaling pathway. Conversely, knockdown of Dmrt-1 by small interfering RNA resulted in cell morphology that was different from that after PMA treatment. These results indicated that Dmrt-1 expression was apparently associated with the differentiation of NEC8, and this cell line may be a helpful in vitro tool to clarify the role of Dmrt-1 in the differentiation process.
authors.affiliation: Department of Environmental and Life Sciences, Kyorin University School of Health Sciences, 876 Miyashita-cho, Hachhioji, Tokyo 192-8508, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Koji
authors.forename: Akira
authors.initials: A
authors.lastname: Yamada
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Nagasawa
authors.forename: Shinobu
authors.initials: S
authors.lastname: Gamou
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D002273:Carcinogens / Q000494:pharmacology; D018236:Carcinoma, Embryonal / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000378:metabolism / Q000473:pathology; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D015398:Signal Transduction; D013737:Testis / Q000378:metabolism / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002273:Carcinogens; D002352:Carrier Proteins; C110547:DMRT1 protein; C493277:MAPKAP1 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014157:Transcription Factors; D011493:Protein Kinase C; D019950:Mitogen-Activated Protein Kinase 1; D013755:Tetradecanoylphorbol Acetate