title: Histological and histochemical changes of the intestinal mucosa at the urothelial-enteric anastomotic site.
abstract: OBJECTIVE
  The incorporation of bowel segments for urinary tract reconstruction may induce intestinal mucosal changes with the development of metabolic, nutritional, gastrointestinal and carcinogenic complications. The early histological and histochemical changes of the intestinal mucosa in contact with the feces-urine mixture, are evaluated in the present study.
  
  
  METHODS
  Twelve rats (operated group) were submitted to a vesico-colonic anastomosis, and 10 rats (control group) underwent a sham operation (the colon was opened and immediately sutured). On the operated group, the left colon was divided into 3 equal portions and the middle segment was used for the bladder-colonic anastomosis. After 20 weeks, the animals were sacrificed and the entire left colon in each group, as well as the bladder and the vesico-colonic anastomosis in the operated group, was removed. The proximal, middle (anastomotic site in the operated group and sutured portion in the control group) and distal colon were used for histological and histochemical studies.
  
  
  RESULTS
  Metaplasia, chronic inflammatory process and fibrosis were significantly greater at the anastomotic site compared to the middle segment of the control group. There were no differences in both groups in terms of dysplasia, atrophy and hypertrophy either on the proximal, middle or anastomotic area and distal portion of the left colon. All animals in the operated group showed a reduced presence of sulfomucin and an increase in the sialomucin content.
  
  
  CONCLUSIONS
  The histological changes observed in this study may suggest a precancerous phenomenon.
authors.affiliation: Division of Urology, State University of Campinas, UNICAMP, Campinas, Sao Paulo, Brazil.
authors.forename: Marcos A
authors.initials: MA
authors.lastname: Castro
authors.forename: Ubirajara
authors.initials: U
authors.lastname: Ferreira
authors.forename: Mario H
authors.initials: MH
authors.lastname: Martins
authors.forename: Rafael M
authors.initials: RM
authors.lastname: Stoppiglia
authors.forename: N Rodrigues
authors.initials: NR
authors.lastname: Netto
mesh_terms: D000714:Anastomosis, Surgical / Q000009:adverse effects; D000818:Animals; D003106:Colon / Q000601:surgery*; D005260:Female; D005355:Fibrosis / Q000473:pathology; D006651:Histocytochemistry; D007249:Inflammation / Q000473:pathology; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology*; D008679:Metaplasia / Q000473:pathology; D009077:Mucins / Q000032:analysis*; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D052116:Sialomucins / Q000032:analysis*; D014513:Ureter / Q000601:surgery*
chemical_list: D009077:Mucins; D052116:Sialomucins; C012564:sulfomucin