title: Prevention of nephrolithiasis by Lactobacillus in stone-forming rats: a preliminary study.
abstract: Hyperoxaluria is a risk factor for renal stones. It appears to be sustained by increased dietary load or increased intestinal absorption. The aim of this study was to evaluate whether oral administration of lactobacilli could prevent urolithiasis in stone-forming rats. Oxalate-degrading activities of lactobacilli were evaluated by measuring the oxalate level in a culture medium after inoculation with lactobacilli. Only the strains of Lactobacillus having oxalate-degrading activity were used. Sprague-Dawley rats were fed a powdered standard diet containing 3% sodium oxalate and/or received 100 mg/kg of celecoxib for the first 8 days by gavage, before or after the beginning of this experiment (groups with previous treatment or with co-treatment). Rats were sacrificed after 4 weeks and kidneys were harvested for the assay of crystal formation under a dissecting microscope. Twenty-four-hour urine collections were performed before kidney harvest. Only two strains, Lactobacillus casei HY2743 and L. casei HY7201 out of 31 strains of Lactobacillus were able to degrade oxalate. In both groups of co-treatment and previous treatment with L. casei HY2743 and L. casei HY7201, urine oxalate excretion decreased compared to the group without lactobacilli. The dissecting microscope examination of kidneys in the rats in two previous treatment groups and the co-treatment group with L. casei HY7201 showed less abundant crystals than control groups. Our results show that lactobacilli may be used as a potential therapeutic strategy in the prevention of urinary stones.
authors.affiliation: Department of Urology, Seoul National University College of Medicine, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.
authors.forename: Cheol
authors.initials: C
authors.lastname: Kwak
authors.forename: Byong Chang
authors.initials: BC
authors.lastname: Jeong
authors.forename: Ja Hyeon
authors.initials: JH
authors.lastname: Ku
authors.forename: Hyeon Hoe
authors.initials: HH
authors.lastname: Kim
authors.forename: Jeong Jun
authors.initials: JJ
authors.lastname: Lee
authors.forename: Chul Sung
authors.initials: CS
authors.lastname: Huh
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Baek
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Lee
mesh_terms: D000818:Animals; D001835:Body Weight; D019343:Citric Acid / Q000652:urine; D003460:Crystallization; D007668:Kidney / Q000378:metabolism; D007669:Kidney Calculi / Q000517:prevention & control*; D007778:Lactobacillus / Q000378:metabolism*; D008297:Male; D010070:Oxalates / Q000378:metabolism* / Q000652:urine; D010865:Pilot Projects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010070:Oxalates; D019343:Citric Acid