title: Immunohistochemical profile and chromosomal imbalances in papillary tumours of the pineal region.
abstract: The histopathology of papillary tumours of the pineal region (PTPR) closely resembles that of ependymomas and choroid plexus tumours. Therefore, immunohistochemical staining profiles were investigated in a series of 15 PTPR. In addition to cytokeratin, synaptophysin and glial fibrillary acidic protein expression, PTPR were examined for the presence of dot- or ring-like epithelial membrane antigen (EMA) immunoreactivity typically encountered in ependymoma, staining for inwardly rectifying potassium channel Kir7.1 and stanniocalcin-1 (specifically expressed in choroid plexus tumours) as well as microtubule-associated protein-2 (MAP-2). Furthermore, comparative genomic hybridization was performed in five PTPR. Cytokeratin was expressed in all PTPR examined, whereas glial fibrillary acidic protein and synaptophysin staining were absent. Dot- or ring-like EMA immunoreactivity was only observed in 1 out of 15 PTPR. Membranous Kir7.1 and cytoplasmic stanniocalcin-1 staining were present in the minority of PTPR (3/15 and 4/15, respectively). In contrast, MAP-2 immunoreactivity was encountered in 13 out of 15 PTPR, but was significantly less frequently observed in a series of choroid plexus tumours (7/37). PTPR mainly presented with chromosomal losses affecting chromosomes 10 (4/5 cases) and 22q (3/5 cases) as well as gains on chromosomes 4 (4/5 cases), 8 (3/5 cases), 9 (3/5 cases) and 12 (3/5 cases). To conclude, the majority of PTPR can be distinguished from ependymomas and choroid plexus tumours by absent staining for epithelial membrane antigen, Kir7.1 and staniocalcin-1 as well as the presence of distinct MAP-2 immunoreactivity. Antibodies directed against these antigens are thus expected to be valuable markers in the diagnosis of papillary tumours located in the vicinity of the third ventricle.
authors.affiliation: Institute of Neuropathology, University Hospital Münster, Münster, Germany. hasselblatt@uni-muenster.de
authors.forename: M
authors.initials: M
authors.lastname: Hasselblatt
authors.forename: I
authors.initials: I
authors.lastname: Blümcke
authors.forename: A
authors.initials: A
authors.lastname: Jeibmann
authors.forename: C H
authors.initials: CH
authors.lastname: Rickert
authors.forename: A
authors.initials: A
authors.lastname: Jouvet
authors.forename: J A P
authors.initials: JA
authors.lastname: van de Nes
authors.forename: K
authors.initials: K
authors.lastname: Kuchelmeister
authors.forename: A
authors.initials: A
authors.lastname: Brunn
authors.forename: M
authors.initials: M
authors.lastname: Fevre-Montange
authors.forename: W
authors.initials: W
authors.lastname: Paulus
mesh_terms: D000231:Adenocarcinoma, Papillary / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002648:Child; D016545:Choroid Plexus Neoplasms / Q000473:pathology; D002869:Chromosome Aberrations; D003937:Diagnosis, Differential; D004806:Ependymoma / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010871:Pinealoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor