title: Modeling Hsp70-mediated protein folding.
abstract: The Hsp70 chaperone system is the major molecular chaperone system that assists protein-folding processes in all cells. To understand these processes, we analyzed the kinetic characteristics of the Escherichia coli homologs of this chaperone system during folding of a denatured protein using computer simulations and compared the results with in vitro refolding experiments. Rate constants used for the model were derived from recent literature or were determined and scrutinized for their applicability to the refolding reaction. Our simulation results are consistent with reported laboratory experiments, not only simulating the refolding reaction of wild-type proteins but also the behavior of mutant variants. Variation of kinetic parameters and concentrations of components of the Hsp70 system demonstrate the robustness of the chaperone system in assisting protein folding. Furthermore, the importance of the synergistic stimulation of the ATPase activity of Hsp70 is demonstrated. The limitations of our kinetic model indicate sore spots in our understanding of this chaperone system. Our model provides a platform for further research on chaperone action and the mechanism of chaperone-assisted refolding of denatured proteins.
authors.affiliation: Institute for Advanced Biosciences, and Graduate School of Media and Governance, Keio University, Tsuruoka, Japan. hubin@sfc.keio.ac.jp
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
authors.forename: Matthias P
authors.initials: MP
authors.lastname: Mayer
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D003198:Computer Simulation; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation; D017510:Protein Folding
chemical_list: D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D000255:Adenosine Triphosphate; C063842:dnaK protein, E coli