title: Adult triclabendazole-resistant Fasciola hepatica: surface and subsurface tegumental responses to in vitro treatment with the sulphoxide metabolite of the experimental fasciolicide compound alpha.
abstract: Mature Fasciola hepatica of the triclabendazole-resistant Sligo isolate were incubated in vitro with 10 microg/ml of the sulphoxide metabolite of compound alpha [5-chloro-2-methylthio-6-(1-naphthyloxy)-H-benzimidazole]; the metabolite will be referred to as alpha.SO. Changes resulting from drug treatment were examined by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and tubulin immunocytochemistry (ICC). SEM revealed that disruption to the tegumental surface mainly took the form of swelling and blebbing. Extensive spine loss occurred on the ventral surface of the oral cone, and sloughing of the tegument was observed along the lateral margins of the fluke. Examination of sections from the anterior mid-body region at the TEM level revealed that treatment with alpha.SO led to swelling of the basal infolds and mitochondria within the tegumental syncytium; also, accumulations of secretory bodies beneath the apical plasma membrane. The tegumental cell bodies contained swollen mitochondria and cisternae of granular endoplasmic reticulum, but few Golgi complexes were observed. An increase in T2 secretory bodies was observed, whilst in the T1 tegumental cells, the T1 secretory bodies had decreased in number. Immunocytochemical (ICC) studies showed that incubation with alpha.SO, ABZ.SO and TCBZ.SO did not cause significant changes to the distribution of tubulin within the tegumental syncytium of the Sligo isolate. In contrast, alpha.SO, ABZ.SO and TCBZ.SO caused severe disruption to tubulin organization within the syncytial layer of the TCBZ-susceptible Cullompton isolate. The EM results confirm that compound alpha is a fasciolicide capable of disrupting the tegument of mature TCBZ-resistant F. hepatica; however, this was not accompanied by any change in tubulin immunoreactivity.
authors.affiliation: Parasite Proteomics and Therapeutics Research Group, School of Biological Sciences, Queens University Belfast, Medical Biology Centre, Belfast, Northern Ireland.
authors.forename: M
authors.initials: M
authors.lastname: McConville
authors.forename: G P
authors.initials: GP
authors.lastname: Brennan
authors.forename: M
authors.initials: M
authors.lastname: McCoy
authors.forename: R
authors.initials: R
authors.lastname: Castillo
authors.forename: A
authors.initials: A
authors.lastname: Hernandez-Campos
authors.forename: F
authors.initials: F
authors.lastname: Ibarra
authors.forename: I
authors.initials: I
authors.lastname: Fairweather
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology; D004351:Drug Resistance; D005210:Fasciola hepatica / Q000187:drug effects* / Q000648:ultrastructure*; D005211:Fascioliasis / Q000188:drug therapy / Q000469:parasitology; D007150:Immunohistochemistry; D008297:Male; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D051381:Rats; D017207:Rats, Sprague-Dawley; D013454:Sulfoxides / Q000494:pharmacology*; D000077682:Triclabendazole; D014404:Tubulin / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D013454:Sulfoxides; D014404:Tubulin; D000077682:Triclabendazole