Bioactive hydroxyphenylpyrrole-dicarboxylic acids from a new marine Halomonas sp.: Production and structure elucidation.
The new marine Halomonas sp. strain GWS-BW-H8hM (DSM 17996) was found to produce 3-(4'-hydroxyphenyl)-4-phenylpyrrole-2,5-dicarboxylic acid (HPPD-1) and 3,4-bis(4'-hydroxy- phenyl)pyrrole-2,5-dicarboxylic acid (HPPD-2). In initial cultivations using marine broth, only low contents of these compounds have been isolated. Improving the conditions and growing the strain on artificial seawater supplemented with tryptone 10 g l(-1), yeast extract 5 g l(-1), L-tyrosine 0.6 g l(-1), glycine 1 g l(-1), and glucose 6 g l(-1), the growth-associated HPPD-1 and HPPD-2 production of a 40-l batch cultivation reached the amounts of 47 mg l(-1) and 116 mg l(-1), respectively, after 65 h. Both compounds showed potent anti-tumor-promoting activities.
Wang
L
L
Institute of Biochemistry and Biotechnology, Technical University of Braunschweig, Spielmannstr. 7, 38106 Braunschweig, Germany.
Grosse
T
T
Stevens
H
H
Brinkhoff
T
T
Simon
M
M
Liang
L
L
Bitzer
J
J
Bach
G
G
Zeeck
A
A
Tokuda
H
H
Lang
S
S
D016588:Anticarcinogenic Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D003998:Dicarboxylic Acids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020632:Halomonas / Q000737:chemistry* / Q000378:metabolism
D016588:Anticarcinogenic Agents; D000970:Antineoplastic Agents; D003998:Dicarboxylic Acids