Improved production of enzymes, which are expressed under the Pho regulon promoter, in the rmf gene (encoding ribosome modulation factor) disruptant of Escherichia coli.
Using a DNA macroarray, we investigated the effects of rmf gene (encoding ribosome modulation factor) disruption on gene expression profiles in Escherichia coli. This strain showed a phosphate-starvation-like response in gene expression even under phosphate sufficient conditions; significant upregulation of the Pho regulon genes was observed. Further, the production of alkaline phosphatase, a product of the Pho regulon gene, phoA, increased in the rmf disruptant under a Pi sufficient condition. Furthermore, production of PhoC acid phosphatase/nucleoside pyrophosphate phosphotransferase derived from Morganella morganii also increased significantly in the rmf disruptant. We concluded that host modification by the rmf gene disruption has potential benefit in industrial enzyme production using Escherichia coli.
Imaizumi
Akira
A
Laboratories of Fermentation and Biotechnology, AJINOMOTO Co., Inc., Kanagawa, Japan. akira_imaizumi@ajinomoto.com
Koseki
Chie
C
Matsui
Kazuhiko
K
Kojima
Hiroyuki
H
D000469:Alkaline Phosphatase / Q000378:metabolism; D001483:Base Sequence; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D008969:Molecular Sequence Data; D010710:Phosphates / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D018085:Regulon / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
D003470:Culture Media; D029968:Escherichia coli Proteins; D010710:Phosphates; D012269:Ribosomal Proteins; C063545:ribosome modulation factor, E coli; D000469:Alkaline Phosphatase