[Expression of heat shock protein 70 and heat shock protein 27 in lens epithelial cells induced by contusion and thermotolerance in rat model].
OBJECTIVE
To study the dynamic expression of heat shock protein (HSP) 70 and HSP27 in lens epithelial cells (LECs) in contused eyes of rat model and to detect the dynamic change of the expression of HSP70 and HSP27 after thermotolerance.


METHODS
Forty-eight Sprague-Dawley (SD) rats were randomized into two groups: contusion group and thermotolerance group. Contusion eyes were induced by dropping a steel ball of 20 g from the height of 20 cm for 100 times. Thermotolerance was induced by raising the core body temperature of rat to 40.5-41.5 degrees C for 8 min, 2-3 h before eyes contusion. HSP70 and HSP27 expression in the LEC were measured using one-tube RT-PCR.


RESULTS
Contusion of eyes resulted in increase of HSP70 expression. Expression of HSP70 increased to 4.59 +/- 0.12 at 1 h after contusion, reached the highest level of 7.72 +/- 0.27 at 3 h after contusion, and reduced to the normal level of 1.32 +/- 0.14 at 24 h after contusion. Preconditioning hyperthermia resulted in significantly increase of HSP70 expression. There was no significant change of HSP27 expression in both groups.


CONCLUSIONS
Increased expression of HSP70 in LECs of contused eyes possibly protects the proteins of lens against degeneration. Thermal preconditioning might suppress lens injury induced by contusion via increasing the expression of HSP70.
Rao
Hui-ying
HY
Eye Center, Affiliated Second Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Yao
Ke
K
Tang
Xia-jing
XJ
Xu
Wen
W
D000818:Animals; D003288:Contusions / Q000378:metabolism / Q000503:physiopathology; D004847:Epithelial Cells / Q000378:metabolism; D005131:Eye Injuries / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006358:Hot Temperature; D007908:Lens, Crystalline / Q000378:metabolism* / Q000502:physiology; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins