title: Purification and characterization of cytosolic 6-phosphogluconate dehydrogenase isozymes from maize.
abstract: Cytosolic isozymes of 6-phosphogluconate dehydrogenase were purified from roots of maize (Zea mays L.). The final preparation contained two 55-kD proteins. Affinity-purified dehydrogenases from a maize line that is null for both cytosolic 6-phosphogluconate dehydrogenase isozymes (Pgd1-null, Pgd2-null) lacked the 55-kD proteins. The substrate kinetics of the purified enzyme were determined.
authors.affiliation: Department of Botany and Plant Sciences, University of California, Riverside, California 92521.
authors.forename: J
authors.initials: J
authors.lastname: Bailey-Serres
authors.forename: M T
authors.initials: MT
authors.lastname: Nguyen