Responses of rat pulp cells to heat stress in vitro.
Although heat stress can cause irritation in the dentin/pulp complex, little is known about the thermotolerance of pulp cells and their response to heat stress. We investigated cultured rat pulp cell responses to heat stress. Cells were subjected to a temperature of 42 degrees C for 30 minutes, and HSPs, alkaline phosphatase activity, and gap-junctional communication were determined at various time points. Although only low levels of HSP70 expression were detected before heat treatment, heat shock markedly induced HSP70 expression, with it gradually increasing at 1 hour after being heated. HSP25, however, showed no dramatic change. Gap junction protein connexin43 rapidly degraded after heat treatment, recovering to normal levels within the following 6 hours. Alkaline phosphatase activity decreased immediately after heat stress, recovering after 1 hour. These results indicate that dental pulp possesses protective factors, including HSPs, and that it can recover viability of intercellular communication and alkaline phosphatase activity after heat stress.
Amano
T
T
Department of Pathology, Tokyo Dental College, Chiba, Japan.
Muramatsu
T
T
Amemiya
K
K
Kubo
K
K
Shimono
M
M
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002450:Cell Communication; D002470:Cell Survival; D002478:Cells, Cultured; D018031:Connexin 43 / Q000096:biosynthesis; D017630:Connexins / Q000096:biosynthesis; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D018882:Heat Stress Disorders / Q000503:physiopathology*; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D006358:Hot Temperature; D007150:Immunohistochemistry; D009363:Neoplasm Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D018031:Connexin 43; D017630:Connexins; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486114:Hspb1 protein, rat; D009363:Neoplasm Proteins; D000469:Alkaline Phosphatase