title: A new steroidal saponin from Agave shrevei.
abstract: A new steroidal saponin was isolated from the leaves of Agave shrevei. Its structure was established as 3-[O-beta-D-glucopyranosyl-(1-->2)-O-[O-beta-D-glucopyranosyl-(1-->4)-O-[O-beta-D-glucopyranosyl-(1-->6)]-O-beta-D-glucopyranosyl-(1-->4)-beta-D-galactopyranosyl)-oxy]-(3beta,5alpha,25R)-spirostane. The structural identification was performed using detailed analyses of 1H- and 13C-NMR spectra including 2D NMR spectroscopic techniques (COSY, HETCOR, HMBC, and HMQC) and chemical conversions. The haemolytic activity of the steroidal saponin was evaluated using an in vitro assay.
authors.affiliation: Laboratório de Química de Plantas Medicinais, Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, PO Box 68045, CEP 21944-971, Rio de Janeiro, Brazil.
authors.forename: Bernadete
authors.initials: B
authors.lastname: Pereira Da Silva
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Valente
authors.forename: José
authors.initials: J
authors.lastname: Paz Parente
mesh_terms: D027882:Agave / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D013150:Spirostans / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: C512673:3-(O-beta-D-glucopyranosyl-(1-2)-O-(O-beta-D-glucopyranosyl-(1-4)-O-(O-beta-D-glucopyranosyl-(1-6))-O-beta-D-glucopyranosyl-(1-4)-beta-D-galactopyranosyl)-oxy)-(3beta,5alpha,25R)-spirostane; D012503:Saponins; D013150:Spirostans