title: Zonal variations in the three-dimensional morphology of the chondron measured in situ using confocal microscopy.
abstract: OBJECTIVE
  Chondrocytes in articular cartilage are surrounded by a narrow pericellular matrix (PCM), which together with the enclosed cell(s) are termed the "chondron". Although the precise function of this tissue region is unknown, previous studies provide indirect evidence that the PCM plays an important role in governing the local mechanical environment of chondrocytes. In particular, theoretical models of the chondron under mechanical loading suggest that the shape, size, and biomechanical properties of the PCM significantly influence the stress-strain and fluid flow environment of the cell. The goal of this study was to quantify the three-dimensional morphology of chondron in situ using en bloc immunolabeling of type VI collagen coupled with fluorescence confocal microscopy.
  
  
  METHODS
  Three-dimensional reconstructions of intact, fluorescently labeled chondrons were made from stacks of confocal images recorded in situ from the superficial, middle, and deep zones of porcine articular cartilage of the medial femoral condyle.
  
  
  RESULTS
  Significant variations in the shape, size, and orientation of chondrocytes and chondrons were observed with depth from the tissue surface, revealing flattened discoidal chondrons in the superficial zone, rounded chondrons in the middle zone, and elongated, multicellular chondrons in the deep zone.
  
  
  CONCLUSIONS
  The shape and orientation of the chondron appear to reflect the local collagen architecture of the interterritorial matrix, which varies significantly with depth. Quantitative measurements of morphology of the chondron and its variation with site, disease, or aging may provide new insights into the influence of this structure on physiology and the pathology of articular cartilage.
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: I
authors.initials: I
authors.lastname: Youn
authors.forename: J B
authors.initials: JB
authors.lastname: Choi
authors.forename: L
authors.initials: L
authors.lastname: Cao
authors.forename: L A
authors.initials: LA
authors.lastname: Setton
authors.forename: F
authors.initials: F
authors.lastname: Guilak
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry / Q000648:ultrastructure*; D019902:Chondrocytes / Q000737:chemistry / Q000648:ultrastructure*; D024142:Collagen Type VI / Q000032:analysis; D005109:Extracellular Matrix / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D007719:Knee Joint; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D013552:Swine; D014021:Tissue Preservation
chemical_list: D024142:Collagen Type VI