MELDB: a database for microbial esterases and lipases.
MELDB is a comprehensive protein database of microbial esterases and lipases which are hydrolytic enzymes important in the modern industry. Proteins in MELDB are clustered into groups according to their sequence similarities based on a local pairwise alignment algorithm and a graph clustering algorithm (TribeMCL). This differs from traditional approaches that use global pairwise alignment and joining methods. Our procedure was able to reduce the noise caused by dubious alignment in the distantly related or unrelated regions in the sequences. In the database, 883 esterase and lipase sequences derived from microbial sources are deposited and conserved parts of each protein are identified. HMM profiles of each cluster were generated to classify unknown sequences. Contents of the database can be keyword-searched and query sequences can be aligned to sequence profiles and sequences themselves.
Kang
Ho-Young
HY
Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, KRIBB, P.O. Box 115, Yuseong, Daejeon 305-600, Republic of Korea.
Kim
Jihyun F
JF
Kim
Myung Hee
MH
Park
Seung-Hwan
SH
Oh
Tae-Kwang
TK
Hur
Cheol-Goo
CG
D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D001665:Binding Sites; D017124:Conserved Sequence; D030562:Databases, Protein; D004950:Esterases / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D008049:Lipase / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny
D004950:Esterases; D008049:Lipase