title: Propionibacterium acnes DNA from an explanted intraocular lens detected by polymerase chain reaction in a case of chronic pseudophakic endophthalmitis.
abstract: We report a case of Propionibacterium acnes DNA from the explanted intraocular lens (IOL) a 76-year-old man with delayed-onset chronic endophthalmitis. The detection was by polymerase chain reaction (PCR). Slitlamp microscopic examination showed cells and flare in the anterior chamber, keratic precipitates on the endothelium, and multiple white plaques on both surfaces of the IOL. Propionibacterium acnes DNA was found in both the aqueous humor and the explanted IOL by PCR. Scanning electron microscopy showed diphtheroid particles, a coating of biofilm-like deposits, as well as scattered crystals on the surface of the explanted IOL and supported the possibility of P acne colonies on the IOL.
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, China.
authors.forename: Jui-Yang
authors.initials: JY
authors.lastname: Lai
authors.forename: Ko-Hua
authors.initials: KH
authors.lastname: Chen
authors.forename: Ying-Cheng
authors.initials: YC
authors.lastname: Lin
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Hsu
authors.forename: Shui-Mei
authors.initials: SM
authors.lastname: Lee
mesh_terms: D000368:Aged; D001082:Aqueous Humor / Q000382:microbiology; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis*; D020878:Device Removal; D009877:Endophthalmitis / Q000382:microbiology* / Q000601:surgery; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology* / Q000601:surgery; D006801:Humans; D007910:Lenses, Intraocular / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction; D011183:Postoperative Complications; D011425:Propionibacterium acnes / Q000235:genetics / Q000302:isolation & purification*; D019591:Pseudophakia / Q000382:microbiology* / Q000601:surgery
chemical_list: D004269:DNA, Bacterial