title: Survival of parvovirus B19-infected cells by cellular autophagy.
abstract: Human parvovirus B19 (B19) is a well-known pathogenic agent which causes apoptosis in erythrocyte lineage cells. Here, we provide the first evidence that mitochondrial autophagy is specifically found in the B19-infected cells. The protein expression ratio for LC3-II/LC3-I increased significantly in infected cells, indicating possible involvement of cellular autophagy in the infection process. Immunofluorescence confocal microscopy analyses revealed that B19 infection induced an intracellular autophagosome as judged by endogenous LC3 staining. Moreover, inhibition of autophagy by 3-MA significantly facilitated B19-infection-mediated cell death. These results suggest a novel mechanism by which B19-infected cells survive by cellular autophagy.
authors.affiliation: Department of Microbiology and Immunology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Akitoshi
authors.initials: A
authors.lastname: Nakashima
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Keiichi
authors.initials: K
authors.lastname: Tamai
authors.forename: Masanao
authors.initials: M
authors.lastname: Kyuuma
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sato
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Yoshimori
authors.forename: Shigeru
authors.initials: S
authors.lastname: Saito
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sugamura
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D001343:Autophagy / Q000502:physiology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival; D003593:Cytoplasm / Q000737:chemistry / Q000648:ultrastructure; D004791:Enzyme Inhibitors / Q000494:pharmacology; D041905:Erythroid Cells / Q000648:ultrastructure / Q000821:virology*; D005434:Flow Cytometry; D006801:Humans; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000032:analysis; D016732:Parvovirus B19, Human / Q000502:physiology*; D010588:Phagosomes / Q000737:chemistry / Q000648:ultrastructure; D017361:Viral Nonstructural Proteins / Q000032:analysis
chemical_list: D004791:Enzyme Inhibitors; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D017361:Viral Nonstructural Proteins; C025946:3-methyladenine; D000225:Adenine