Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2alpha.
Prostaglandin F2alpha (PGF2alpha) induces cyclin D1 expression and DNA synthesis in Swiss 3T3 cells. In order to assess which signaling mechanisms are implicated in these processes, we have used both a pharmacological approach and interfering mutants. We demonstrate that PGF2alpha induces extracellular-signal-regulated kinase (ERK1-2) and p38MAPK activation, and inhibition of any of these signaling pathways completely blocks PGF2alpha-stimulated DNA synthesis. We also show that ERK1-2, but not p38MAPK activation is required to induce cyclin D1 expression, strongly suggesting that the concerted action of cyclin D1 gene expression and other events are required to induce complete phosphorylation of retinoblastoma protein and S-phase entry in response to PGF2alpha.
Dekanty
Andrés
A
Fundación Instituto Leloir, Av. Patricias Argentinas 435, 1405 Buenos Aires, Argentina.
Giulianelli
Sebastián
S
Coso
Omar A
OA
Rudland
Philip S
PS
Jimenez de Asua
Luis
L
D000818:Animals; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects*; D019938:Cyclin D1 / Q000235:genetics; D004261:DNA Replication; D015237:Dinoprost / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D041701:Swiss 3T3 Cells
D019938:Cyclin D1; D015237:Dinoprost; D020928:Mitogen-Activated Protein Kinases