[Effects of cordyceps sinensis alcohol extractive on serum interferon-gamma level and splenic T lymphocyte subset in mice with viral myocarditis].
OBJECTIVE
To investigate the effects of early application of cordyceps sinensis alcohol extractive (CSAE) on myocardial injury, level of serum IFN-gamma and splenic T lymphocyte subset in murine viral myocarditis (VMC).


METHODS
100 male BALB/c mice were divided randomly into control group (CG), infectious group (IG) and CSAE therapy group (CTG). Mice in IG and CTG were infected with Coxsackie virus B3 (CVB3). The 14-day survival rates and myocardial histopathology were observed. Serum IFN-gamma level was detected by ELISA and splenic CD3+, CD4+, CD8+ T lymphocytes were detected by flow cytometry.


RESULTS
Serum IFN-gamma level and the percentage of splenic T lymphocyte subset in IG were decreased significantly, and CD4+/CD8+ T cell ratio increased significantly as compared with those of CG. Myocardial lesions in CIG were attenuated, while the 14-day survival of mice 85% (vs IG 55%, P<0.05), serum IFN-gamma and the percentage of splenic CD3+, CD8+ T lymphocytes in CTG were significantly higher than those in IG. There were no significant differences of the percentage of T lymphocyte subset and CD4+/CD8+ T cell ratio between CTG and CG.


CONCLUSIONS
CSAE plays an important protective role against viral-induced murine myocarditis by inducing IFN-gamma and regulating T lymphocyte.
Li
Feng
F
The Department of Cardiology, Shandong Province Hospital, Shandong University, Jinan 250021, China. jnlf@sina.com
Gao
Xing-yu
XY
Rao
Bang-fu
BF
Liu
Li
L
Dong
Bo
B
Cui
Lian-qun
LQ
D000818:Animals; D032661:Cordyceps / Q000737:chemistry*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D000431:Ethanol / Q000737:chemistry; D007371:Interferon-gamma / Q000097:blood*; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000097:blood / Q000188:drug therapy* / Q000821:virology; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D016176:T-Lymphocyte Subsets / Q000187:drug effects*; D014777:Virus Diseases / Q000188:drug therapy
D004365:Drugs, Chinese Herbal; D000431:Ethanol; D007371:Interferon-gamma