title: Effects of heat shock protein gp96 on human dendritic cell maturation and CTL expansion.
abstract: We reported previously that heat shock protein gp96 and its N-terminal fragment were able to stimulate CTL expansion specific for a HBV peptide (SYVNTNMGL) in BALB/c mice. Here we characterized the adjuvant effects of gp96 on human HLA-A2 restricted T cells. Full-length gp96 isolated from healthy human liver and recombinant fragments both from prokaryotic cells and eukaryotic cells were analyzed for their ability to stimulate maturation of human dendritic cells. It was found that in vitro these proteins were capable of maturating human monocyte-derived dendritic cells (MDDC) isolated from healthy donors as well as from HBV-positive, hepatocellular carcinoma (HCC) patients. In HLA-A2.1/Kb transgenic mice, gp96 and the recombinant fragments were found to augment CTL response specific for the HBcAg(18-27) FLPSDFFPSV peptide of hepatitis B virus.
authors.affiliation: Molecular Virology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanlu
authors.initials: Y
authors.lastname: Zan
authors.forename: Ming
authors.initials: M
authors.lastname: Shan
authors.forename: Changmei
authors.initials: C
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Fusheng
authors.initials: F
authors.lastname: Wang
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhong
authors.forename: Fulian
authors.initials: F
authors.lastname: Liao
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
authors.forename: Po
authors.initials: P
authors.lastname: Tien
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000473:pathology*; D006360:Heat-Shock Proteins / Q000008:administration & dosage; D006509:Hepatitis B / Q000473:pathology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000473:pathology*
chemical_list: D006360:Heat-Shock Proteins; D008562:Membrane Glycoproteins; C053387:endoplasmin