Processing body autoantibodies reconsidered.
Processing bodies (P-bodies) are cellular structures that have critical roles in mRNA degradation and post-transcriptional gene silencing. Some patients with autoimmune disease have high titer antibodies directed against P-bodies, and certain sera have been used as markers for the GW182 component of these structures. This study shows that available reference sera are unreliable markers for GW182 because the sera contain antibodies directed against Ge-1, a second P-body autoantigen.
Bloch
Donald B
DB
Gulick
Tod
T
Bloch
Kenneth D
KD
Yang
Wei-Hong
WH
D060565:Argonaute Proteins; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D001324:Autoantigens / Q000737:chemistry* / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000473:pathology; D045744:Cell Line, Tumor; D015852:Eukaryotic Initiation Factor-2; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007211:Indoles; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology; D009687:Nuclear Proteins / Q000276:immunology; D010448:Peptide Initiation Factors / Q000276:immunology; D011506:Proteins; D012333:RNA, Messenger / Q000378:metabolism*; D034743:RNA-Induced Silencing Complex / Q000378:metabolism*
C466422:AGO2 protein, human; D060565:Argonaute Proteins; D001323:Autoantibodies; D001324:Autoantigens; C507914:EDC4 protein, human; D015852:Eukaryotic Initiation Factor-2; D005456:Fluorescent Dyes; D007211:Indoles; D009687:Nuclear Proteins; D010448:Peptide Initiation Factors; D011506:Proteins; D012333:RNA, Messenger; D034743:RNA-Induced Silencing Complex; D049452:Green Fluorescent Proteins; C007293:DAPI