title: Expression of phosphorylated Stat3, cyclin D1 and Bcl-xL in extramammary Paget disease.
abstract: BACKGROUND
  Stat3 (Signal transducer and activator of transcription-3) is an oncogene that plays a critical role in regulating fundamental processes associated with malignant transformation and cell survival. It participates in oncogenesis through upregulation of genes encoding apoptosis inhibitors (Bcl-xL) and cell cycle regulators (cyclin D1). The expression of Stat3, Bcl-xL and cyclin D1 protein has not been investigated in extramammary Paget disease (EMPD).
  
  
  OBJECTIVE
  To study the expression of phosphorylated Stat3 (p-Stat3), Bcl-xL and cyclin D1 protein in EMPD and to evaluate the relationships among them.
  
  
  METHODS
  Thirty-six tissue samples from 34 patients with primary EMPD were subjected to immunohistochemical staining for p-Stat3, cyclin D1 and Bcl-xL.
  
  
  RESULTS
  Thirty-five of 36 specimens were clearly positive for p-Stat3 in EMPD, while 30 of 36 and 32 of 36 were positive for cyclin D1 and Bcl-xL expression, respectively. In all of four invasive EMPD specimens, strong and frequent expression of these three molecules was evident; moreover, two invasive EMPD specimens with lymph nodal metastasis showed very strong nuclear and membranous p-Stat3 staining. Two metastatic lymph node specimens showed very strong nuclear and local membrane p-Stat3 staining. There were significant correlations between p-Stat3 and cyclin D1 expression and between p-Stat3 and Bcl-xL expression.
  
  
  CONCLUSIONS
  Our study shows that the expression of p-Stat3, cyclin D1 and Bcl-xL may play a pivotal role in the pathogenesis of EMPD.
authors.affiliation: Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashiku, Fukuoka 812-8582, Japan, and Department of Dermatology, Union Hospital, Hubei, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Moroi
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: S
authors.initials: S
authors.lastname: Yasumoto
authors.forename: H
authors.initials: H
authors.lastname: Kokuba
authors.forename: S
authors.initials: S
authors.lastname: Imafuku
authors.forename: T
authors.initials: T
authors.lastname: Koga
authors.forename: T
authors.initials: T
authors.lastname: Tetsuya
authors.forename: Y T
authors.initials: YT
authors.lastname: Tu
authors.forename: H
authors.initials: H
authors.lastname: Aburatani
authors.forename: M
authors.initials: M
authors.lastname: Furue
authors.forename: K
authors.initials: K
authors.lastname: Urabe
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019938:Cyclin D1 / Q000378:metabolism; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D010145:Paget Disease, Extramammary / Q000378:metabolism* / Q000473:pathology / Q000556:secondary; D010766:Phosphorylation; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D012867:Skin / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: D009363:Neoplasm Proteins; D050796:STAT3 Transcription Factor; D051020:bcl-X Protein; D019938:Cyclin D1