title: Doppler ultrasonographic and scintigraphic assessment of an auxiliary heterotopic liver transplantation with portal vein arterialization in pigs.
abstract: OBJECTIVE
  Our aim was to evaluate liver graft integrity and function using scintigraphy and ultrasonography in a porcine model of auxiliary heterotopic liver transplantation with portal vein arterialization (AHLT-PVA).
  
  
  METHODS
  Using Doppler ultrasonography we evaluated eight AHLT-PVA by parenchymal echogenicity, portal and arterial anatomy, and portal and biliary system flow. Two types of scintigraphy were performed: microaggregated human albumin colloid scintigraphy and diisopropyl iminodiacetic acid (DISIDA) scintigraphy, both labeled with 99mTc.
  
  
  RESULTS
  The animals were distributed into two groups. The first group consisted of three animals with clinical suspicion of graft dysfunction, in which the ultrasonographic study revealed areas of parenchymal destructuring. In the scintigraphic study, heterogenous uptake was observed; there was no uptake in one animal. Necropsy of these three animals revealed areas of graft necrosis. The second group consisted of five animals with good clinical evolutions, in which the ultrasonographic study showed portal dilation, portal flow with arterial spiculations, and homogenous echogenicity of the hepatic parenchyma. The scintigraphic study revealed homogenous uptake by the graft and an elimination speed of the hepatobiliary agent similar to that of the native liver.
  
  
  CONCLUSIONS
  An heterogenous echostructure of the graft provided a sign of poor prognosis indicating necrosis in the same way as heterogenous uptake or nonuptake of radioisotope upon scintigraphy. Scintigraphy is a good method to evaluate biliary function and bile elimination. In an AHLT-PVA, the main ultrasound findings derived from arterialization were dilation of the portal system and portal flow with arterial spiculations.
authors.affiliation: Unit of Experimental Surgery, Department of Surgery, Arrixaca University Hospital, Murcia, Spain. olga005ma@terra.es
authors.forename: O M
authors.initials: OM
authors.lastname: Fernández-Rodríguez
authors.forename: A
authors.initials: A
authors.lastname: Ríos
authors.forename: J L
authors.initials: JL
authors.lastname: Navarro
authors.forename: J A
authors.initials: JA
authors.lastname: Pons
authors.forename: C G
authors.initials: CG
authors.lastname: Palenciano
authors.forename: R
authors.initials: R
authors.lastname: Mota
authors.forename: J J
authors.initials: JJ
authors.lastname: Berenguer
authors.forename: F
authors.initials: F
authors.lastname: Mulero
authors.forename: J
authors.initials: J
authors.lastname: Contreras
authors.forename: C
authors.initials: C
authors.lastname: Conesa
authors.forename: P
authors.initials: P
authors.lastname: Ramírez
authors.forename: T
authors.initials: T
authors.lastname: Fuente
authors.forename: P
authors.initials: P
authors.lastname: Parrilla
mesh_terms: D000818:Animals; D008099:Liver / Q000000981:diagnostic imaging*; D016031:Liver Transplantation / Q000379:methods* / Q000502:physiology; D023421:Models, Animal; D011169:Portal Vein / Q000601:surgery*; D011877:Radionuclide Imaging; D013552:Swine; D016042:Transplantation, Heterotopic; D018608:Ultrasonography, Doppler