[Functionalization of aminomodified probes for atomic force microscopy].
Probes for atomic force microscopy functionalized by bovine serum albumin were obtained, which may be used for molecular recognition studies. The procedure of the modification and functionalization of probes includes three stages. First, amino probes are obtained by modification in vapors of amino silane derivative. Then a homobifunctional amino reactive cross-linker is covalently linked to surface amino groups of the amino probe. And finally, the probe with the covalently attached cross-linker is functionalized by bovine serum albumin molecules. The probes obtained were characterized at different stages of the modification by atomic force microscopy: the adhesion force and the work of adhesion force were determined from histograms. The modification of probe surface was confirmed by visualization of bovine serum albumin and supercoiled pGEMEX DNA molecules immobilized on the amino mica and amino mica modified by cross-linker.
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D000588:Amines / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D004278:DNA, Superhelical / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force / Q000379:methods; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D000588:Amines; D003432:Cross-Linking Reagents; D015342:DNA Probes; D004278:DNA, Superhelical; D012710:Serum Albumin, Bovine