title: Construction and characterization of Bacillus subtilis deletion mutants lacking the prophage 2-trnS region.
abstract: During development of a novel method for constructing a series of deletions in Bacillus subtilis using an isogenic set of gene-disrupted mutants created by integration of pMutin, deletion of the trnS operon, consisting of seven tRNA genes, was found to affect cell growth, development of competence and spore formation. A suppressor (sts1) of the DeltatrnS mutant was isolated, sequenced and found to have undergone a single base change, CAG to GAG, in the first anticodon of tRNA(Leu), in the trnB operon.
authors.affiliation: Laboratory of Molecular Genetics and Frontier Project Life's Adaptation Strategies to Environmental Changes, Department of Life Science, College of Science, Rikkyo University, Tokyo, Japan.
authors.forename: Genki
authors.initials: G
authors.lastname: Akanuma
authors.forename: Chiemi
authors.initials: C
authors.lastname: Habu
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Natori
authors.forename: Rikinori
authors.initials: R
authors.lastname: Murayama
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nanamiya
authors.forename: Fujio
authors.initials: F
authors.lastname: Kawamura
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D001483:Base Sequence; D005798:Genes, Bacterial; D009876:Operon; D039002:Prophages / Q000235:genetics; D012356:RNA, Transfer, Leu / Q000235:genetics; D011995:Recombination, Genetic; D017384:Sequence Deletion; D013171:Spores, Bacterial; D013489:Suppression, Genetic
chemical_list: D012356:RNA, Transfer, Leu