Preparation of radioactive gibberellin a(1) and its metabolism in dwarf peas.
Gibberellin A(1)-3,4-(3)H was prepared by selective catalytic reduction of gibberellic acid with a mixture of tritium and hydrogen. (3)H-GA(1) was applied at physiological concentrations to dwarf peas and the metabolism of the hormone was investigated. (3)H-GA(1) was converted to an acidic, biologically active compound. Radioactive but biologically inactive compounds were also found in the neutral fraction and could not be converted to acidic gibberellins by hydrolysis. No attachment of gibberellin to any macromolecular fraction was evident.
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MSU/AEC Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48823.