Enantiomeric separation of mineralocorticoid receptor (hMR) antagonists using the Chiralcel OJ-H HPLC column with novel polar cosolvent eluent systems.
This study demonstrates the increased versatility of the Chiralcel OJ-H stationary phase when using various alcohol/acetonitrile mobile phases. This chiral stationary phase has traditionally been employed in the normal phase mode and more recently with neat alcohols as eluents. Selected isomeric human mineralocorticoid receptor (hMR) antagonist pharmaceutical candidates and synthetic intermediates were separated using the Chiralcel OJ-H HPLC column with novel polar cosolvent eluent systems. The capacity factors, resolution, and selectivity of the chiral separations were assessed while varying the alcohol/acetonitrile composition and alcohol identity. The mixed polar eluents provide separations that are nearly always superior to both the traditional hexane-rich and single-alcohol "polar organic" eluents for the compounds tested in this article.
Sharp
V Scott
VS
Lilly Research Laboratories, Eli Lilly & Company, Indianapolis, IN 46285, USA. vss@lilly.com
Kennedy
Joseph H
JH
Belvo
Matthew D
MD
Williams
Jeffrey D
JD
Risley
Donald S
DS
Seest
Eric P
EP
D000097:Acetonitriles / Q000737:chemistry; D000438:Alcohols / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D000451:Mineralocorticoid Receptor Antagonists; D015394:Molecular Structure; D008970:Molecular Weight; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism
D000097:Acetonitriles; D000438:Alcohols; D000451:Mineralocorticoid Receptor Antagonists; D004364:Pharmaceutical Preparations; D012997:Solvents; C070231:Chiracel OJ; D002482:Cellulose; C032159:acetonitrile