Functionalized surface arrays for spatial targeting of immune cell signaling.
The effect of surface topography and chemistry on cellular response is of fundamental importance, especially where living systems encounter device surfaces as in medical implants, tissue engineering, and cell-based sensors. To understand these biological processes on surfaces, there is a widespread interest in tailored surface-active materials produced by a combination of surface chemistry coupled to advanced patterning processes. We utilize self-assembled monolayers (SAMs) as molecular templates with submicrometer-scale spatial resolution to engage and cluster IgE receptors on rat basophilic leukemia (RBL) mast cells. Bioactive templates consisted of gold arrays on silicon with patterns from 1 mum down to 45 nm. These gold arrays served as molecular tethering sites, enabling covalent binding of functionalized self-assembled monolayers of alkanethiols. The free ends of the monolayers were functionalized with 2,4-dinitrophenyl(DNP)-caproate-based ligands which interact specifically with anti-DNP IgE bound to its high affinity cell surface receptor, FcepsilonRI on RBL mast cells. Present results on structures 1 mum down to 600 nm in size indicate that these ligand-immobilized patterned arrays can function as a powerful tool for visualization and systematic characterization of cell membrane involvement in IgE receptor-mediated immune cell signaling.
Senaratne
Wageesha
W
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
Sengupta
Prabuddha
P
Jakubek
Vladimir
V
Holowka
David
D
Ober
Christopher K
CK
Baird
Barbara
B
D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015398:Signal Transduction; D013499:Surface Properties; D013601:T-Lymphocytes / Q000276:immunology*