Antibody responses and epitope specificities to the Taenia solium cysticercosis vaccines TSOL18 and TSOL45-1A.
Taenia solium is a cestode parasite that causes cysticercosis in humans and pigs. This study examined the antibody responses in pigs immunized with the TSOL18 and TSOL45-1A recombinant vaccines against T. solium cysticercosis. Immunization with these proteins induced specific, complement-fixing antibodies against the recombinant antigens that are believed to be associated with vaccine-induced protection against T. solium infection. Sera from immunized pigs were used to define the linear B-cell epitopes of TSOL18 and TSOL45-1A. Prominent reactivity was revealed to one linear epitope on TSOL18 and two linear epitopes on TSOL45-1A. These, and oncosphere antigens from other taeniid cestodes, contain a protein sequence motif suggesting that they may show a tertiary structure similar to the fibronectin type III domain (FnIII). Comparison of the location of linear antigenic epitopes in TSOL18 and TSOL45-1A within the proposed FnIII structure to those within related cestode vaccine antigens reveals conservation in the positioning of the epitopes between oncosphere antigens from different taeniid species.
Kyngdon
C T
CT
Veterinary Clinical Centre, The University of Melbourne, Werribee, Victoria, Australia. c.kyngdon@pgrad.unimelb.edu.au
Gauci
C G
CG
Gonzalez
A E
AE
Flisser
A
A
Zoli
A
A
Read
A J
AJ
Martínez-Ocaña
J
J
Strugnell
R A
RA
Lightowlers
M W
MW
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D003551:Cysticercosis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology*; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D041201:Taenia solium / Q000235:genetics / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018985:Epitopes, B-Lymphocyte; C113407:TSOL-18 antigen; D014614:Vaccines, Synthetic