Effect of estrogen on the expression of occludin in ovariectomized mouse brain.
The blood-brain barrier (BBB) and blood-cerebrospinal fluid barrier (BCSFB), formed by interactions of tight junctions (TJs) in the endothelia or the choroids plexus, respectively, are important diffusion barriers between systemic circulation and neural tissue of the central nervous system (CNS). Epidemiological incidence of degenerative changes in the CNS is significantly higher in postmenopausal women than in men, suggesting an important role of ovarian steroids for CNS integrity in women. To elucidate the role estrogen in the maintenance of paracellular diffusion barriers in the CNS, changes in the expression of occludin, a TJ protein, following ovariectomy (OVX), as well as the effect of estrogen on the expression of occludin in OVX female brain, were examined in mice. Immunoreactivity of occludin was found in brain endothelial capillaries, choroids plexus, ependyma, and aqueduct. In OVX brain, expression of occludin mRNA and protein was decreased a little but significantly relative to a sham control brain at the estrous stage. Estrogen (17beta estradiol) significantly up-regulated occludin mRNA levels in OVX mice. Together, these results suggest that occludin is an important structural element in both the BBB and BCSFB, and that expression of occludin in female brain tissue is sensitive to estrogen levels.
Kang
Han S
HS
Department of Life Science, College of Natural Sciences, Hanyang University, Haengdang-Dong, Seongdong-Gu, Seoul 133-791, South Korea.
Ahn
Hae S
HS
Kang
Hee J
HJ
Gye
Myung C
MC
D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D007150:Immunohistochemistry / Q000379:methods; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D062793:Occludin; D010052:Ovariectomy / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
D004967:Estrogens; D008565:Membrane Proteins; D062793:Occludin; C568986:Ocln protein, mouse; D012333:RNA, Messenger