title: Dereplication of pentacyclic triterpenoids in plants by GC-EI/MS.
abstract: Three common plant-derived pentacyclic triterpenoids, oleanolic acid (1), betulinic acid (2) and ursolic acid (3), have been found to exhibit moderate anti-tubercular activity in a microplate alamar blue assay. In order to facilitate the discovery of novel anti-tubercular leads with diverse chemical structures, a new and rapid GC-EI/MS method was developed simultaneously and unambiguously to dereplicate 1-3 as their methyl esters with limits of detection of 25.6, 26.9 and 26.8 ng, respectively.
authors.affiliation: Department of Pharmacology and Toxicology, Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Arizona, Tucson, AZ 85721, USA.
authors.forename: Jian-Qiao
authors.initials: JQ
authors.lastname: Gu
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Wang
authors.forename: Scott G
authors.initials: SG
authors.lastname: Franzblau
authors.forename: Gloria
authors.initials: G
authors.lastname: Montenegro
authors.forename: Barbara N
authors.initials: BN
authors.lastname: Timmermann
mesh_terms: D000995:Antitubercular Agents / Q000737:chemistry*; D002849:Chromatography, Gas; D027523:Lavandula / Q000737:chemistry*; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000737:chemistry*; D053978:Pentacyclic Triterpenes; D021241:Spectrometry, Mass, Electrospray Ionization; D020903:Tanacetum parthenium / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry*; D014632:Valerian / Q000737:chemistry*; D000094062:Betulinic Acid; D000097245:Ursolic Acid
chemical_list: D000995:Antitubercular Agents; D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D000094062:Betulinic Acid; D000097245:Ursolic Acid