Peroxidases from the extreme dwarf tomato plant. Identification, isolation, and partial purification.
Utilizing starch-gel electrophoresis, 12 discrete peroxidases were identified in purified extracts of the dwarf tomato shoot (Lycopersicon esculentum). Nine of the peroxidases moved toward the anode while 3 moved toward the cathode. In addition, a continuous peroxidase smear was separated from the above 12 peroxidases. This smear moved toward the anode and accounted for 50% of the total peroxidase activity in the crude extract.Four of the major peroxidases were isolated and partially purified, using acetone and ammonium sulfate precipitations, preparative starch-gel electrophoresis and chromatography on DEAE and CM-cellulose.
Evans
J J
JJ
Department of Biology and Biology Field Station, Case Western Reserve University, Cleveland, Ohio 44106.