title: Involvement of 2-C-methyl-D-erythritol-4-phosphate pathway in biosynthesis of aphidicolin-like tetracyclic diterpene of Scoparia dulcis.
abstract: Specific inhibitors of the MVA pathway (pravastatin) and the MEP pathway (fosmidomycin) were used to interfere with the biosynthetic flux which leads to the production of aphidicolin-like diterpene in leaf organ cultures of Scoparia dulcis. Treatment of leaf organs with fosmidomycin resulted in dose dependent inhibition of chlorophylls, carotenoids, scopadulcic acid B (SDB) and phytol production, and no effect on sterol production was observed. In response to the pravastatin treatment, a significant decrease in sterol and perturbation of SDB production was observed.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Marguerite Kasidimoko
authors.initials: MK
authors.lastname: Nkembo
authors.forename: Jung-Bum
authors.initials: JB
authors.lastname: Lee
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakagiri
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D016590:Aphidicolin / Q000096:biosynthesis*; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000378:metabolism*; D004896:Erythritol / Q000031:analogs & derivatives* / Q000378:metabolism; D005578:Fosfomycin / Q000031:analogs & derivatives / Q000494:pharmacology; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000378:metabolism; D017035:Pravastatin / Q000494:pharmacology; D036721:Scoparia / Q000187:drug effects / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013261:Sterols / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism*
chemical_list: C114232:2-C-methylerythritol 4-phosphate; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D004224:Diterpenes; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D013261:Sterols; D013403:Sugar Phosphates; D002734:Chlorophyll; D005578:Fosfomycin; D002338:Carotenoids; D016590:Aphidicolin; C024640:fosmidomycin; D017035:Pravastatin; D004896:Erythritol