title: [Chemiluminescent detection of E. coli O157:H7 in food based on magnetic enzyme-linked immuno method].
abstract: OBJECTIVE
  To establish a new chemiluminescent assay with high sensitivity for detecting E. coli O157:H7 in food.
  
  
  METHODS
  The assay was established based on the alkaline phosphatase (ALP) labeled anti-E. coli O157:H7 antibody, which reacted with an excellent chemiluminescent reagent, 3(2' spiroadamantane)4 methoxy 4(3''phosphoryloxy) phenyl 1,2 dioxetane (AMPPD) buffer solution and emitted the photons.
  
  
  RESULTS
  The sensitivity of chemiluminescence assay was 850, and the linear range was 1000-50,000. The intra- and interassay CV values (CVs) were respectively below 15% and 20%. The correlation coefficient was 0.9807 by comparing with the conventional counting method.
  
  
  CONCLUSIONS
  The chemiluminescent immunoassay is more accurate than other methods in the detection of concentration of E. coli O157:H7 in samples.
authors.affiliation: State Key Laboratory for Bioelectronics, Southeast University, Nanjing 210096, China.
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Guo
authors.forename: Yong-ning
authors.initials: YN
authors.lastname: Wang
authors.forename: Lin-gang
authors.initials: LG
authors.lastname: Gu
authors.forename: He-ping
authors.initials: HP
authors.lastname: Liu
authors.forename: Yan-feng
authors.initials: YF
authors.lastname: He
authors.forename: Zu-hong
authors.initials: ZH
authors.lastname: Lu
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019453:Escherichia coli O157 / Q000276:immunology / Q000302:isolation & purification*; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology / Q000592:standards; D007118:Immunoassay; D008163:Luminescent Measurements / Q000379:methods*; D012680:Sensitivity and Specificity