Effects of degU32(Hy), degQa and degR pleiotropic regulatory genes on the growth and protease fermentation of Bacillus subtilis Ki-2-132.
Effects of degU32 (Hy), degR genes from Bacillus subtilis 168 and degQa gene from Bacillus amyloliquefaciens on Bacillus subtilis Ki-2-132 cell growth, sporulation and protease fermentation were investigated by introducing these genes into B. subtilis Ki-2-132 chromosome and/or cytoplasm. Although the genes come from different species and strains, they showed pleiotropic effects in B. subtilis Ki-2-132. B. subtilis Ki-2-132degU32 (Hy) showed increased protease production, and when cooperating with degQa either in plasmid or in chromosome, further altered cell growth, increased protease production and affected the spore formation in a glucose and dosage dependent manner. By contrast, degR did not significantly affect the protease productivity in degU32 (Hy) mutant, consisting with that DegR was used to stabilise DegU-phosphate, which in degU32 (Hy) strain no longer further amplify the DegU-phosphate effect.
Pan
Xue-Feng
XF
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. xuefengpancam@yahoo.com.cn
D001412:Bacillus subtilis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D049108:Cell Enlargement; D002876:Chromosomes, Bacterial / Q000235:genetics; D010450:Endopeptidases / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009154:Mutation; D013171:Spores, Bacterial / Q000235:genetics; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; C066497:DegU protein, Bacteria; D010450:Endopeptidases; C087822:alkaline protease