title: Refinement of cytokine use in the in vitro expansion of erythroid cells.
abstract: Blood transfusion is indispensable for many clinical applications. However, the supply of transfusable material is insufficient in many countries. Human cord blood contains many hematopoietic stem and progenitor cells, providing a promising resource for the production of transfusable material in vitro. In this study, we have refined a protocol to produce abundant red blood cells (RBC) from human cord blood in an in vitro culture system. We found that erythropoietin and interleukin-3 were most effective when they were added to the culture medium sequentially rather than simultaneously. Although insulin-like growth factor-I (IGF-1) has been reported to function as a positive regulator of RBC production in some in vitro culture systems, we found that IGF-1 had a negative effect upon RBC production. However, IGF-II appeared to function as a positive regulator of RBC production. Finally, stem cell factor functioned to both expand and accelerate the differentiation of immature erythroid cells.
authors.affiliation: Cell Engineering Division, RIKEN BioResource Center, University of Tsukuba, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Miharada
authors.forename: Takashi
authors.initials: T
authors.lastname: Hiroyama
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sudo
authors.forename: Toshiro
authors.initials: T
authors.lastname: Nagasawa
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000008:administration & dosage / Q000494:pharmacology*; D004912:Erythrocytes / Q000166:cytology*; D004920:Erythropoiesis / Q000187:drug effects*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D013002:Somatomedins / Q000494:pharmacology; D013268:Stimulation, Chemical
chemical_list: D016207:Cytokines; D013002:Somatomedins