title: Factors derived from mouse embryonic stem cells promote self-renewal of goat embryonic stem-like cells.
abstract: Goat embryonic stem (ES)-like cells could be isolated from primary materials-inner cell masses (ICMs) and remain undifferentiated for eight passages in a new culture system containing mouse ES cell conditioned medium (ESCCM) and on a feeder layer of mouse embryo fibroblasts (MEFs). However, when cultured in medium without mouse ESCCM, goat ES-like cells could not survive for more than three passages. In addition, no ES-like cells could be obtained when ICMs were cultured on goat embryo fibroblasts or the primary materials-whole goat blastocysts were cultured on MEFs. Goat ES-like cells isolated from ICMs had a normal karyotype and highly expressed alkaline phosphatase. Multiple differentiation potency of the ES-like cells was confirmed by differentiation into neural cells and fibroblast-like cells in vitro. These results suggest that mouse ES cells might secrete factors playing important roles in promoting goat ES-like cells' self-renewal, moreover, the feeder layers and primary materials could also influence the successful isolation of goat ES-like cells.
authors.affiliation: Shanghai Transgenic Research Center, Shanghai 201203, China.
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Tian
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Sha
authors.forename: Xu-Jun
authors.initials: XJ
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: You-Bing
authors.initials: YB
authors.lastname: Wu
authors.forename: Sheng-Hua
authors.initials: SH
authors.lastname: Cheng
authors.forename: Jian-Quan
authors.initials: JQ
authors.lastname: Chen
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Shi
authors.forename: Zeng-Liang
authors.initials: ZL
authors.lastname: Bai
authors.forename: Guo-Xiang
authors.initials: GX
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D017077:Culture Media, Conditioned; D004622:Embryo, Mammalian / Q000166:cytology; D006041:Goats / Q000196:embryology*; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned