title: Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle.
abstract: We described the development of functionalized magnetic nanoparticles (MNPs) with PEG-modification, a phospholipids micelle coating, and their use in manipulating histidine-tagged proteins. Highly monodisperse MNPs were synthesized in an organic solvent and could be phase-transferred into an aqueous solution by encapsulating the nanoparticles with a phospholipids micelle. The phospholipids micelle coating rendered the nanoparticles highly water-soluble, and the functional groups of the phospholipids coating allowed for the bioconjugation of various moieties, such as fluorescent molecules and engineered proteins. Functionalized phospholipids, such as nitrilotriacetic acid (NTA)-phospholipids, caused the MNPs to bind and allowed for manipulation of histidine-tagged proteins. Due to their high surface/volume ratio, the MNPs showed better performance (about 100 times higher) in immobilizing engineered proteins than conventional micrometer-sized beads. This demonstrates that MNPs coated with phospholipids micelle can be a versatile platform for the effective manipulation of various kinds of engineered proteins, which is very important in the field of proteomics. It is expected that a combination of MNPs with optical fluorescent molecules can find applications in bimodal (magnetic and optical) molecular imaging nanoprobes.
authors.affiliation: Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors.forename: Yong Taik
authors.initials: YT
authors.lastname: Lim
authors.forename: Kun Yeong
authors.initials: KY
authors.lastname: Lee
authors.forename: Kwangyeol
authors.initials: K
authors.lastname: Lee
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000528:radiation effects; D006639:Histidine / Q000737:chemistry* / Q000528:radiation effects; D008280:Magnetics; D008823:Micelles; D009038:Motion; D049329:Nanostructures / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure*; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure
chemical_list: D020099:Coated Materials, Biocompatible; D008823:Micelles; D010743:Phospholipids; D011994:Recombinant Proteins; D006639:Histidine; D009571:Nitrilotriacetic Acid