Detection of a Cytosolic Glutamine Synthetase in Leaves of Nicotiana tabacum L. by Immunocytochemical Methods.
Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells.
Carvalho
H
H
Centro de Citologia Experimental (Instituto Nacional de Investigação Científica), Universidade do Porto, 4100-Porto, Portugal.
Pereira
S
S
Sunkel
C
C
Salema
R
R