title: A functional network involved in the recycling of nucleocytoplasmic pre-60S factors.
abstract: Eukaryotic pre-ribosomes go through cytoplasmic maturation steps before entering translation. The nucleocytoplasmic proteins participating in these late stages of maturation are reimported to the nucleus. In this study, we describe a functional network focused on Rei1/Ybr267w, a strictly cytoplasmic pre-60S factor indirectly involved in nuclear 27S pre-ribosomal RNA processing. In the absence of Rei1, the nuclear import of at least three other pre-60S factors is impaired. The accumulation in the cytoplasm of a small complex formed by the association of Arx1 with a novel factor, Alb1/Yjl122w, inhibits the release of the putative antiassociation factor Tif6 from the premature large ribosomal subunits and its recycling to the nucleus. We propose a model in which Rei1 is a key factor for the coordinated dissociation and recycling of the last pre-60S factors before newly synthesized large ribosomal subunits enter translation.
authors.affiliation: Génétique des Interactions Macromoléculaires, Centre National de la Recherche Scientifique URA2171, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Lebreton
authors.forename: Cosmin
authors.initials: C
authors.lastname: Saveanu
authors.forename: Laurence
authors.initials: L
authors.lastname: Decourty
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Rain
authors.forename: Alain
authors.initials: A
authors.lastname: Jacquier
authors.forename: Micheline
authors.initials: M
authors.lastname: Fromont-Racine
mesh_terms: D021581:Active Transport, Cell Nucleus; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011132:Polyribosomes / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020798:Two-Hybrid System Techniques
chemical_list: C000607951:Alb1 protein, S cerevisiae; D002352:Carrier Proteins; D007381:Intermediate Filament Proteins; D026901:Membrane Transport Proteins; C117894:NMD3 protein, S cerevisiae; C483822:PSE1 protein, S cerevisiae; D010750:Phosphoproteins; C511835:REI1 protein, S cerevisiae; D016601:RNA-Binding Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C491609:TIF6 protein, S cerevisiae; C036298:ribosomal protein L24; C031127:ribosomal protein L25