Applications of highly sensitive phosphopeptide derivatization methods without the need for organic solvents.
Detection and identification of phosphorylated amino acid residues is still one of the most challenging tasks in contemporary protein analysis. Especially serine- and threonine-phosphorylation is very often involved in the regulation of enzyme activities and regulates key functions in cellular processes.
Reinders
Joerg
J
Protein Mass Spectrometry and Functional Proteomics Group, Rudolf-Virchow-Center for Experimental Biomedicine, Julius-Maximilians-Universität Würzburg, Würzburg, Germany.
Meyer
Helmut E
HE
Sickmann
Albert
A
D002853:Chromatography, Liquid; D003543:Cysteamine / Q000737:chemistry; D003903:Deuterium; D004229:Dithiothreitol / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D010748:Phosphopeptides / Q000032:analysis / Q000737:chemistry*; D010766:Phosphorylation; D012997:Solvents / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D009930:Organic Chemicals; D010748:Phosphopeptides; D012997:Solvents; D003543:Cysteamine; D003903:Deuterium; D004229:Dithiothreitol