Prolactin inhibits the apoptosis of chondrocytes induced by serum starvation.
The apoptosis of chondrocytes plays an important role in endochondral bone formation and in cartilage degradation during aging and disease. Prolactin (PRL) is produced in chondrocytes and is known to promote the survival of various cell types. Here we show that articular chondrocytes from rat postpubescent and adult cartilage express the long form of the PRL receptor as revealed by immunohistochemistry of cartilage sections and by RT-PCR and Western blot analyses of the isolated chondrocytes. Furthermore, we demonstrate that PRL inhibits the apoptosis of these same chondrocytes cultured in low-serum. Chondrocyte apoptosis was measured by hypodiploid DNA content determined by flow cytometry and by DNA fragmentation evaluated by the ELISA and the TUNEL methods. The anti-apoptotic effect of PRL was dose-dependent and was prevented by heat inactivation. These data demonstrate that PRL can act as a survival factor for chondrocytes and that it has potential preventive and therapeutic value in arthropathies characterized by cartilage degradation.
Zermeño
C
C
Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, 07360, México.
Guzmán-Morales
J
J
Macotela
Y
Y
Nava
G
G
López-Barrera
F
F
Kouri
J B
JB
Lavalle
C
C
de la Escalera
G Martínez
GM
Clapp
C
C
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002358:Cartilage, Articular / Q000166:cytology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000502:physiology*; D053938:DNA Fragmentation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling / Q000379:methods; D008297:Male; D011388:Prolactin / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D011981:Receptors, Prolactin / Q000008:administration & dosage / Q000032:analysis
D012333:RNA, Messenger; D011981:Receptors, Prolactin; D011388:Prolactin