title: Observations on the mechanism of copper damage in chlorella.
abstract: Addition of excess copper to nongrowing cells of a normal, green Chlorella caused a reduction in total pigments and a blue shift of chlorophyll absorption, concurrent with the inhibition of photosynthesis. Chlorophylless yellow and white mutant strains of the same alga showed a rise in nonspecific absorption (i.e., change in light scatter) within 5 to 10 minutes after the addition of CuSO(4); concomitantly a lowering of packed cell volume and a rise in respiration occurred. Glutathione prevented all copper-induced changes, whereas MnCl(2) protected only partially. Selective inhibition of some responses to copper was observed when O(2) was absent or an antioxidant present.
authors.affiliation: Department of Life Sciences, University of California, Riverside, California 92502.
authors.forename: R E
authors.initials: RE
authors.lastname: Gross
authors.forename: P
authors.initials: P
authors.lastname: Pugno
authors.forename: W M
authors.initials: WM
authors.lastname: Dugger