Microarray analysis of transcription factor gene expression in melatonin-treated human peripheral blood mononuclear cells.
The existence of specific melatonin-binding sites in lymphoid cells led to the discovery of signal transduction pathway for melatonin in human lymphocytes and immunomodulatory role of melatonin in immune cells. In recent years, transcriptional regulation of melatonin on various transcription factors has been demonstrated. Therefore, this study was designed to assess by cDNA microarray analysis the regulatory effects of melatonin on transcription factors in human peripheral blood mononuclear cells (PBMCs). Forty-six genes were upregulated and 23 were downregulated more than twofold in melatonin-treated PBMCs. Of the more than twofold upregulated transcription factor genes, homeo box A4 (HOXA4), forkhead box O1A (FOXO1A), transcription elongation factor B (SIII), polypeptide 3 (TCEB3), and peroxisome proliferative activated receptor delta (PPARD) were identified. Of the more than twofold downregulated genes, PHD finger protein 15 (PHF15) and zinc finger protein 33a (ZNF33A) were identified. In summary, identification of these genes by cDNA microarray analysis in response to melatonin administration may provide a foundation for further studies on the function of melatonin in human PBMCs.
Ha
Eunyoung
E
Department of Biochemisty, Kohwang Medical Research Institute, College of Medicine, Kyung Hee University, Seoul, Korea.
Han
Earl
E
Park
Hae Jeong
HJ
Kim
Hak-Jae
HJ
Hong
Mee Suk
MS
Hong
Seung-Jae
SJ
Yoon
Kyung Sik
KS
Kang
Insug
I
Cho
Yong Hoo
YH
Chung
Joo-Ho
JH
Yim
Sung-Vin
SV
Baik
Hyung Hwan
HH
D001483:Base Sequence; D017931:DNA Primers; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*
D017931:DNA Primers; D014157:Transcription Factors