title: Embryonic stem cell-derived neural progenitors display temporal restriction to neural patterning.
abstract: Neural stem cells have considerable therapeutic potential because of their ability to generate defined neuronal cell types for use in drug screening studies or cell-based therapies for neurodegenerative diseases. In this study, we differentiate mouse embryonic stem cells to neural progenitors with an initial forebrain identity in a defined system that enables systematic manipulation to generate more caudal fates, including motoneurons. We demonstrate that the ability to pattern embryonic stem cell-derived neural progenitors is temporally restricted and show that the loss of responsiveness to morphogenetic cues correlates with constitutive expression of the basic helix-loop-helix transcription factors Olig2 and Mash1, epidermal growth factor receptor, and vimentin and parallels the onset of gliogenesis. We provide evidence for two temporal classes of embryonic stem cell-derived putative radial glia that coincide with a transition from neurogenesis to gliogenesis and a concomitant loss of regional identity.
authors.affiliation: Neurobiology Programme, The Babraham Institute, Babraham, Cambridge, United Kingdom.
authors.forename: Isabelle A
authors.initials: IA
authors.lastname: Bouhon
authors.forename: Alexis
authors.initials: A
authors.lastname: Joannides
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Kato
authors.forename: Siddharthan
authors.initials: S
authors.lastname: Chandran
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Allen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
chemical_list: D016895:Culture Media, Serum-Free