Intracellular Salmonella enterica redirect exocytic transport processes in a Salmonella pathogenicity island 2-dependent manner.
During intracellular life, Salmonella enterica proliferate within a specialized membrane compartment, the Salmonella-containing vacuole (SCV), and interfere with the microtubule cytoskeleton and cellular transport. To characterize the interaction of intracellular Salmonella with host cell transport processes, we utilized various model systems to follow microtubule-dependent transport. The vesicular stomatitis virus glycoprotein (VSVG) is a commonly used marker to follow protein transport from the Golgi to the plasma membrane. Using a VSVG-GFP fusion protein, we observed that virulent intracellular Salmonella alter exocytotic transport and recruit exocytotic transport vesicles to the SCV. This virulence function was dependent on the function of the type III secretion system encoded by Salmonella Pathogenicity Island 2 (SPI2) and more specifically on a subset of SPI2 effector proteins. Furthermore, the Golgi to plasma membrane traffic of the shingolipid C(5)-ceramide was redirected to the SCV by virulent Salmonella. We propose that Salmonella modulates the biogenesis of the SCV by deviating this compartment from the default endocytic pathway to an organelle that interacts with the exocytic pathway. This observation might reveal a novel element of the intracellular survival and replication strategy of Salmonella.
Kuhle
Volker
V
Institut für Klinische Mikrobiologie, Immunologie und Hygiene, FAU Erlangen-Nürnberg, Wasserturmstr. 3-5, D-91054 Erlangen, Germany.
Abrahams
Garth L
GL
Hensel
Michael
M
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001693:Biological Transport, Active; D002451:Cell Compartmentation; D002460:Cell Line; D005089:Exocytosis; D044404:Genomic Islands; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008870:Microtubules / Q000378:metabolism / Q000382:microbiology; D008954:Models, Biological; D021381:Protein Transport; D019779:Salmonella enterica / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D022142:Secretory Vesicles / Q000378:metabolism / Q000382:microbiology; D015398:Signal Transduction; D014617:Vacuoles / Q000378:metabolism / Q000382:microbiology; D014759:Viral Envelope Proteins / Q000378:metabolism
D001426:Bacterial Proteins; C028391:G protein, vesicular stomatitis virus; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C440638:SPI-2 protein, Salmonella; C421862:SifA protein, Salmonella; D014759:Viral Envelope Proteins