Light-induced de Novo Synthesis of Ribulose 1,5-Diphosphate Carboxylase in Greening Leaves of Barley.
An antibody specific for ribulose 1,5-diphosphate carboxylase was used to isolate the enzyme from greening barley (Hordeum vulgare L.) leaves. The increase in enzymatic activity during greening was due to de novo synthesis of the enzyme. Increases in enzymatic activity were accompanied by corresponding increases in enzyme protein and by incorporation of radioactive leucine, all of which were inhibited by low concentrations of cycloheximide. (14)C-Labeled amino acids were incorporated into the enzyme by covalent peptide bonding.
Kleinkopf
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GE
Department of Agronomy and Range Science, and Department of Veterinary Microbiology, University of California, Davis, California 95616.
Huffaker
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RC
Matheson
A
A