Inhibition of nifedipine-induced proliferation of cultured human gingival fibroblasts by Saiko, a Chinese herbal medicine.
Saiko is predominantly contained in Saireito, a Chinese herbal medicine. The present study was conducted to determine whether or not Saiko is involved in the inhibition by Saireito of nifedipine-induced proliferation and collagen synthesis in gingival fibroblasts. Nifedipine (10 microM) significantly enhanced the proliferation starting on day 5 of the culture period. When added together with nifedipine, Saiko at concentrations of 0.05%-0.2% (w/v) dose-dependently inhibited the nifedipine-induced proliferation, and at the highest concentration tested (0.2%), Saiko inhibited the nifedipine-induced proliferation by about 40%. Moreover, Saiko (0.2%) also inhibited the normal proliferation at days 11 and 14. Sole application of nifedipine (10 microM) augmented the release of bFGF, and Saiko concentration-dependently reduced the level of bFGF in the nifedipine-containing culture medium. Nifedipine (10 microM) increased the production of type I collagen to almost twice that of the control (normal medium), and Saiko at concentrations above 0.1% significantly reduced the nifedipineinduced production of collagen. In conclusion, the present findings demonstrate that Saiko inhibited the nifedipine-induced proliferation of gingival fibroblasts by reducing the release of bFGF and that Saiko is involved in the Saireito-induced inhibition of nifedipine-stimulated proliferation and collagen synthesis in gingival fibroblasts.
Hattori
Toshimi
T
Department of Dental Pharmacology, Matsumoto Dental University, 1780 Hirooka-Gohbara, Shiojiri 399-0781, Japan. hattori@po.mdu.ac.jp
Matsunaga
Shozo
S
Nakazono
Youdai
Y
Wang
Pao-Li
PL
D029972:Bupleurum; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005881:Gingiva; D006801:Humans; D009543:Nifedipine / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*
D017077:Culture Media, Conditioned; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D014665:Vasodilator Agents; D016222:Fibroblast Growth Factor 2; D009543:Nifedipine