title: A highly flexible tRNA acylation method for non-natural polypeptide synthesis.
abstract: Here we describe a de novo tRNA acylation system, the flexizyme (Fx) system, for the preparation of acyl tRNAs with nearly unlimited selection of amino and hydroxy acids and tRNAs. The combination of the Fx system with an appropriate cell-free translation system allows us to readily perform mRNA-encoded synthesis of proteins and short polypeptides involving multiple non-natural amino acids.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 153-8904 Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ohta
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ashigai
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suga
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis*; D015202:Protein Engineering / Q000379:methods*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D046249:Transfer RNA Aminoacylation
chemical_list: D000596:Amino Acids; D009842:Oligopeptides; D012333:RNA, Messenger; D012343:RNA, Transfer