[Effects of inosine injection in expression of myosin heavy chain in intrafusal and extrafusal myofibers in soleus muscle of tail-suspended rats].
OBJECTIVE
To study the effects of inosine on myosin heavy chain (MHC) activity in rats soleus muscle after tail-suspension.


METHODS
Weightlessness was simulated by tail-suspension of rats. Using immunohistochemistry technique, changes of expression of MHC in intrafusal and extrafusal fibers in soleus muscle were detected.


RESULTS
Expression of fast MHC in intrafusal and extrafusal fibers in soleus muscle increased after 14 d of simulated weightlessness, whereas in tail-suspended rats given inosine, the expression of fast MHC was not detected.


CONCLUSIONS
Inosine could counteract the changes in expression of MHC in intrafusal and extrafusal fibers of rats soleus under simulated weightless situation.
Shi
Lei
L
Department of Physical Education, Xi'an 710061, China. shilei@mail.xjtu.edu.cn
Fan
Xiao-li
XL
Wu
Su-di
SD
D000818:Animals; D015870:Gene Expression; D019417:Hindlimb Suspension; D007267:Injections; D007288:Inosine / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018474:Weightlessness Simulation
D007288:Inosine; D018995:Myosin Heavy Chains