Can Taenia solium latent post-oncospheral stages be found in muscle tissue of cysticercosis-infected pigs (Sus scrofa)?
The existence of latent Taenia solium post-oncospheral stages in the tissues of infected pigs has been postulated. To assess whether such structures exist and can be detected, we examined muscle samples from cysticercosis-infected and uninfected pigs. Pork samples were homogenized, centrifuged, and resuspended in saline solution. Round microscopic structures of approximately 10 microm with variable refringence were found in the pellets of all samples from both infected and uninfected pigs. These became homogeneously red after staining with Sudan IV and disappeared after ether extraction. The only difference between samples from infected and uninfected pigs was the presence of inflammatory cells and tissue necrosis debris in the former group. Taenia solium oncospheres were stained and observed for comparative purposes, before and after inoculation into pork. Control oncospheres were ellipsoidal, had nucleated basophile cells in their interior, and showed red aggregates on their surfaces when stained with 3% Sudan IV. While rounded microscopical structures similar to those previously reported were found, these differed morphologically from oncospheres, were of a lipid nature, and occurred in both infected and uninfected animals. No evidence supporting the presence of latent post-oncospheral stages of Taenia solium was generated in this series of experiments.
Rodrìguez
Mary L
ML
Department of Microbiology, Universidad Peruana Cayetano Heredia, Av. H. Delgado 430, SMP, Lima 31, Lima, Peru.
Rodriguez
Silvia
S
Gonzalez
Armando E
AE
Verastegui
Manuela
M
Bernal
Teresa
T
Jimenez
Juan A
JA
Garcia
Hector H
HH
D000818:Animals; D002499:Centrifugation, Density Gradient; D003551:Cysticercosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D005260:Female; D008018:Life Cycle Stages / Q000502:physiology; D008297:Male; D018482:Muscle, Skeletal / Q000469:parasitology*; D034421:Sus scrofa / Q000469:parasitology*; D013553:Swine Diseases / Q000469:parasitology* / Q000473:pathology; D041201:Taenia solium / Q000166:cytology / Q000302:isolation & purification*; D013997:Time Factors