title: Oligomeric forms of peptide fragment PrP(214-226) in solution are preferentially recognized by PrP(Sc)-specific antibody.
abstract: A specific monoclonal antibody (mAb) V5B2 that discriminates between brain tissue of Creutzfeldt-Jakob disease patients and that from normal controls without proteinase K digestion has been prepared using a 13-residue synthetic peptide P1 from the primary structure of human PrP. In the light of the specific interaction between mAb V5B2 and the pathological isoform of PrP (PrP(Sc)), we investigated the solution behavior of antigen P1 and its interactions with mAb V5B2. Our results show that V5B2 recognizes epitope P1 in dimeric/oligomeric forms in solution and in the fibril-like aggregates, as well as in PrP(Sc) aggregates, and demonstrate that the specific epitope is present in all of these forms, but not in PrP(C).
authors.affiliation: Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia. natasa.poklar@bf.uni-lj.si
authors.forename: Natasa Poklar
authors.initials: NP
authors.lastname: Ulrih
authors.forename: Mihaela
authors.initials: M
authors.lastname: Skrt
authors.forename: Peter
authors.initials: P
authors.lastname: Veranic
authors.forename: Vesna
authors.initials: V
authors.lastname: Galvani
authors.forename: Tanja
authors.initials: T
authors.lastname: Vranac
authors.forename: Vladka
authors.initials: V
authors.lastname: Curin Serbec
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D007562:Creutzfeldt-Jakob Syndrome / Q000175:diagnosis*; D019281:Dimerization; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016056:Immunodominant Epitopes / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D018620:PrPSc Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D011328:Prions / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D012996:Solutions / Q000737:chemistry; D013696:Temperature
chemical_list: D000911:Antibodies, Monoclonal; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D018620:PrPSc Proteins; D011328:Prions; D020033:Protein Isoforms; D012996:Solutions; C511802:prion protein (214-226)