RFLP analysis for mitochondrial genome of CMS-rice.
Restriction fragment length polymorphism (RFLP) was used to analyze mitochondrial (mt) genome of cytoplasmic male sterility (CMS) rice. Differences were observed among mitochondrial genomes of the sterile line (A) and maintain line (B) of nine types of CMS rice; Mitochondrial genomic differences were also detected between A and B in many functional gene regions. Even the materials with the same nucleic background have differences in their mtDNA. This provides molecular evidence for the cytoplasmic heterogeneity and the CMS mechanism research.
Huang
Wei
W
Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, China.
Wang
Li
L
Yi
Ping
P
Tan
Xue-Lin
XL
Zhang
Xue-Mei
XM
Zhang
Zai-Jun
ZJ
Li
Yang-Sheng
YS
Zhu
Ying-Guo
YG
D003433:Crosses, Genetic; D003593:Cytoplasm / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D018740:Genetic Heterogeneity; D018745:Genome, Plant / Q000235:genetics; D006824:Hybridization, Genetic; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D051479:Plant Infertility / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D004272:DNA, Mitochondrial; D018744:DNA, Plant