title: Investigations of the modular structure of bacterial promoters.
abstract: Bacterial RNA polymerase holoenzyme carries different determinants that contact different promoter DNA sequence elements. These contacts are essential for the recognition of promoters prior to transcript initiation. Here, we have investigated how active promoters can be built from different combinations of elements. Our results show that the contribution of different contacts to promoter activity is critically dependent on the overall promoter context, and that certain combinations of contacts can hinder transcription initiation.
authors.affiliation: School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK.
authors.forename: Nora S
authors.initials: NS
authors.lastname: Miroslavova
authors.forename: Stephen J W
authors.initials: SJ
authors.lastname: Busby
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D046911:Macromolecular Substances; D012321:DNA-Directed RNA Polymerases