Molecular diagnosis of culture-negative cerebral nocardiosis due to Nocardia abscessus.
We describe a case of culture-negative cerebral nocardiosis in a 34-year-old immunocompetent man who presented multiple cerebral abscesses. All bacteriologic cultures were negative. Nocardiosis was diagnosed by using a direct genus-specific 16S rDNA amplification method, and Nocardia abscessus was identified by hsp65 sequence analysis. The patient is alive and well on imipenem and doxycycline therapy, 14 months after onset.
Marchandin
Hélène
H
Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 34295 Montpellier Cedex 5, France. h-marchandin@chu-montpellier.fr
Eden
Aurélia
A
Jean-Pierre
Hélène
H
Reynes
Jacques
J
Jumas-Bilak
Estelle
E
Boiron
Patrick
P
Laurent
Frédéric
F
D000328:Adult; D001922:Brain Abscess / Q000175:diagnosis* / Q000382:microbiology; D005784:Gene Amplification / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009615:Nocardia / Q000145:classification / Q000235:genetics; D009617:Nocardia Infections / Q000175:diagnosis* / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014057:Tomography, X-Ray Computed
D012336:RNA, Ribosomal, 16S