Effect of different flavonoids on collagen synthesis in human fibroblasts.
In this study, we discovered that flavonoids belonging to the subclasses: (flavanone, flavone, and flavonol) display differential effects on the synthesis of collagen in human dermal fibroblasts. At 80 microg/ml flavonoids quercetin-3,3',4', 5,7-pentahydroxyflavone, 3-methyl quercetin, and 7-hydroxyflavone significantly decreased the total protein concentration which was a direct consequence of their cytotoxic effect, while naringenin exhibited no effect on total collagen and total protein concentration. Quercetin-3,3'4',7-tetramethyl ether, 4'-hydroxyflavanone, flavanone, and fisetin significantly decreased collagen concentration while morin, rutin, and chrysin increased collagen concentration without changing the overall protein concentration. The initial screening performed in this study enables the identification of compounds that exert significant effects on fibroblast function and show potential as starting material for pharmaceutical preparations targeted against various disorders centered around disturbed collagen metabolism.
Stipcevic
Tamara
T
Department of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia. tamara@irb.hr
Piljac
Jasenka
J
Vanden Berghe
Dirk
D
D003094:Collagen / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D044950:Flavanones / Q000494:pharmacology; D047309:Flavones / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols / Q000494:pharmacology; D006801:Humans; D013329:Structure-Activity Relationship; D046509:Tissue Culture Techniques
D044950:Flavanones; D047309:Flavones; D005419:Flavonoids; D044948:Flavonols; D003094:Collagen