title: Lysosomal, cytoskeletal, and metabolic alterations in cardiomyopathy of cathepsin L knockout mice.
abstract: Although lysosomal proteases are expressed in the heart at considerable levels, their specific functions in this organ remain elusive. Mice deficient for the lysosomal cysteine protease cathepsin L (CTSL) develop a late onset dilated cardiomyopathy (DCM) that is characterized by cardiac chamber dilation, fibrosis, and impaired cardiac contraction at 12 month of age. Investigation of the pathogenic sequence of DCM in ctsl-/- mice revealed numerous dysmorphic lysosome-like structures in heart muscle as early as 3 days after birth, whereas skeletal muscle was not affected. Labeling of the acidic cell compartment of neonatal cardiomyocytes and detection of lysosomal markers after subcellular fractionation confirmed increased lysosome content in CTSL deficient myocardium; however, specific storage materials were not detected. The myocardium of ctsl+/+ and ctsl-/- mice revealed no differences in incidence of cell death, proliferation, and capillary density during DCM progression. However, an observed increase in mRNA expression of natriuretic peptides in young adult mice indicates the activation of the adaptive "fetal" gene program, while proteome analysis revealed decreased levels of the sarcomere-associated proteins alpha-tropomyosin, desmin, and calsarcin 1, as well as considerable changes of metabolic enzymes. Bioinformatic pathway analysis suggested a switch to anaerobic catabolism and impairment of mitochondrial respiration. This interpretation was supported by a 50% reduction in resting state oxygen consumption and impaired respiration capacity in ctsl-/- myocardial homogenates. In summary, the data indicate an essential role of CTSL in maintaining the structure of the endosomal/lysosomal compartment in cardiomyocytes. Lysosomal impairment in ctsl-/- hearts results in metabolic and sarcomeric alterations that promote DCM development.
authors.affiliation: Institut für Molekulare Medizin und Zellforschung, Albert-Ludwigs-Universität Freiburg, Freiburg D-79104, Germany.
authors.forename: Ivonne
authors.initials: I
authors.lastname: Petermann
authors.forename: Christian
authors.initials: C
authors.lastname: Mayer
authors.forename: Jörg
authors.initials: J
authors.lastname: Stypmann
authors.forename: Martin L
authors.initials: ML
authors.lastname: Biniossek
authors.forename: Desmond J
authors.initials: DJ
authors.lastname: Tobin
authors.forename: Markus A
authors.initials: MA
authors.lastname: Engelen
authors.forename: Thomas
authors.initials: T
authors.lastname: Dandekar
authors.forename: Tilman
authors.initials: T
authors.lastname: Grune
authors.forename: Lorenz
authors.initials: L
authors.lastname: Schild
authors.forename: Christoph
authors.initials: C
authors.lastname: Peters
authors.forename: Thomas
authors.initials: T
authors.lastname: Reinheckel
mesh_terms: D000818:Animals; D002311:Cardiomyopathy, Dilated / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D056668:Cathepsin L; D002403:Cathepsins / Q000235:genetics / Q000502:physiology*; D016923:Cell Death; D049109:Cell Proliferation; D019069:Cell Respiration; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000502:physiology*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D018450:Disease Progression; D005355:Fibrosis; D008247:Lysosomes / Q000737:chemistry / Q000648:ultrastructure*; D051379:Mice; D018345:Mice, Knockout; D009200:Myocardial Contraction; D009206:Myocardium / Q000201:enzymology* / Q000473:pathology* / Q000648:ultrastructure; D018384:Oxidative Stress; D020543:Proteome / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D020543:Proteome; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D056668:Cathepsin L; C534278:Ctsl protein, mouse