Evidence of cyanamide production in hairy vetch Vicia villosa.
Cyanamide (NH(2)CN) has recently been isolated as a plant growth inhibitor from Vicia villosa, which is the first discovery of cyanamide from natural sources. To reveal the presence of the biosynthesized cyanamide in plants, 3.4 mM potassium ((15)N)nitrate was administered to 15- to 35-day-old plants of V. villosa, from which the cyanamide was purified and subjected to GC/MS analysis. The isotopic ratio (15)N/((14)N + (15)N) of the cyanamide was calculated to be 0.143, while that of the cyanamide extracted from V. villosa grown in the presence of a natural N source was 0.0065. The (15)N-enrichment proved de novo biosynthesis of cyanamide.
Kamo
Tsunashi
T
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399-4598, Japan. kamo274@shinshu-uac.jp
Kato
Kenji
K
Hiradate
Syuntaro
S
Nakajima
Eri
E
Fujii
Yoshiharu
Y
Hirota
Mitsuru
M
D003484:Cyanamide / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009566:Nitrates / Q000493:pharmacokinetics; D009587:Nitrogen Isotopes / Q000493:pharmacokinetics; D008517:Phytotherapy; D010937:Plant Growth Regulators / Q000096:biosynthesis* / Q000737:chemistry; D031306:Vicia / Q000378:metabolism*
D009566:Nitrates; D009587:Nitrogen Isotopes; D010937:Plant Growth Regulators; D003484:Cyanamide