A plant miRNA contributes to antibacterial resistance by repressing auxin signaling.
Plants and animals activate defenses after perceiving pathogen-associated molecular patterns (PAMPs) such as bacterial flagellin. In Arabidopsis, perception of flagellin increases resistance to the bacterium Pseudomonas syringae, although the molecular mechanisms involved remain elusive. Here, we show that a flagellin-derived peptide induces a plant microRNA (miRNA) that negatively regulates messenger RNAs for the F-box auxin receptors TIR1, AFB2, and AFB3. Repression of auxin signaling restricts P. syringae growth, implicating auxin in disease susceptibility and miRNA-mediated suppression of auxin signaling in resistance.
Navarro
Lionel
L
Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
Dunoyer
Patrice
P
Jay
Florence
F
Arnold
Benedict
B
Dharmasiri
Nihal
N
Estelle
Mark
M
Voinnet
Olivier
O
Jones
Jonathan D G
JD
D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism; D005408:Flagellin / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D035683:MicroRNAs / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000254:growth & development / Q000472:pathogenicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000502:physiology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014170:Transformation, Genetic
C000596911:AFB3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D044783:F-Box Proteins; D007210:Indoleacetic Acids; D035683:MicroRNAs; D012333:RNA, Messenger; D018749:RNA, Plant; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C501405:TIR1 protein, Arabidopsis; D005408:Flagellin