Inhibition of putrescine aminopropyltransferase influences rat liver regeneration.
A close relationship between rat liver regeneration and the concentration ratio of spermidine to spermine (spd:spm) was demonstrated by the oral administration of trans-4-methylcyclohexylamine (MCHA), a specific inhibitor of putrescine aminopropyltransferase. A decrease in recovery rate of remnant liver with MCHA, as a percentage index of remnant liver weight to body weight, correlated well with a decrease of the spd:spm value, with a correlation coefficient of 0.952 for the remnant livers on day 3 after partial hepatectomy. The decrease in recovery rate could be explained by a prolonged cell cycle based on the data of the proliferating cell nuclear antigen labelling index and mitotic cell index in both livers of day 2 and day 3 after partial hepatectomy. The results presented here will give a new aspect in the field of polyamine regulation to control cell growth in vivo.
Kobayashi
Masaki
M
Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
Takao
Koichi
K
Shiota
Yasuo
Y
Sugita
Yoshiaki
Y
Takahashi
Masakazu
M
Nakae
Dai
D
Samejima
Keijiro
K
D000818:Animals; D015317:Biogenic Polyamines / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003514:Cyclohexylamines / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D008938:Mitosis / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011436:Spermidine Synthase / Q000037:antagonists & inhibitors*
D015317:Biogenic Polyamines; D003514:Cyclohexylamines; D004791:Enzyme Inhibitors; C059116:4-methylcyclohexylamine; D011436:Spermidine Synthase