title: Growth inhibition of tobacco tissue cultures with 6-azauracil, 6-azauridine and maleic hydrazide.
abstract: Tissue cultures of Nicotiana tabacum, Nicotiana suaveolens and Nicotiana suaveolens x Nicotiana langsdorffii were cultured in the presence of the growth inhibitors maleic hydrazide and 6-azauracil as well as 6-azauridine. Inhibition of growth was complete at 10(-4) molar concentrations in all 3 genotypes of cells. The inhibition due to 6-azauracil could be completely relieved with uridine and partially relieved with uracil. The effect with maleic hydrazide was irreversible, a fact which indicates a different mechanism or level of inhibition. This study supports the concept that derivatives of 6-azauracil inhibit the synthesis of uridine derivatives required for ribose nucleic acid synthesis and growth.
authors.affiliation: Crops Research Division, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland.
authors.forename: G W
authors.initials: GW
authors.lastname: Schaeffer
authors.forename: T
authors.initials: T
authors.lastname: Sorokin