title: Complex clonal features in an mycobacterium tuberculosis infection in a two-year-old child.
abstract: The focus of this report is to describe the highly complex clonal features of the Mycobacterium tuberculosis (MTB) isolates cultured from a 2-year-old child with no risk factors for tuberculosis. Molecular analysis showed that she was coinfected by 2 MTB isolates. The coinfecting isolates showed only subtle genetic differences and shared equivalent infectivity in vitro; however, they were infecting independent anatomic sites (compartmentalized infection). One of the MTB isolates was cultured only from respiratory specimens, whereas the other was isolated from extrarespiratory site (central nervous system).
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital Gregorio Marañón, Madrid, Spain. dgviedma@microb.net
authors.forename: Darío
authors.initials: D
authors.lastname: García de Viedma
authors.forename: Mercedes
authors.initials: M
authors.lastname: Marín
authors.forename: Sandra
authors.initials: S
authors.lastname: Andrés
authors.forename: Gema
authors.initials: G
authors.lastname: Lorenzo
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Ruiz-Serrano
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
mesh_terms: D015373:Bacterial Typing Techniques; D002555:Cerebrospinal Fluid / Q000382:microbiology; D002675:Child, Preschool; D019143:Evolution, Molecular; D005260:Female; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009841:Oligonucleotides / Q000032:analysis; D012137:Respiratory System / Q000382:microbiology; D014390:Tuberculosis, Meningeal / Q000382:microbiology*
chemical_list: D009841:Oligonucleotides