title: Induction of neuroserpin expression in rat frontal cortex after chronic antidepressant treatment and electroconvulsive treatment.
abstract: Using expressed sequence tag (EST) analysis, we previously identified certain molecular machinery that mediates antidepressant effects. To date, several partial cDNA fragments, termed antidepressant-related genes (ADRGs), have been isolated as ESTs from rat brain. In the present study, we identified two of the ADRGs to be rat neuroserpin. Using real-time quantitative PCR, we demonstrated increased neuroserpin mRNA expression in rat frontal cortex after chronic treatment with several classes of antidepressants, including imipramine, fluoxetine, sertraline, and venlafaxine. Electroconvulsive treatment (ECT), another therapeutic treatment for depression, also increased neuroserpin expression in rat frontal cortex. Neuroserpin is a serine protease inhibitor that is implicated in the regulation of synaptic plasticity, neuronal migration, and axogenesis in the central nervous system. In conclusion, our results support the hypothesis that neuroserpin-mediated plastic changes in frontal cortex may underlie the therapeutic action of antidepressants and ECT.
authors.affiliation: Department of Psychiatry, Showa University School of Medicine.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tanaka
authors.forename: Misa
authors.initials: M
authors.lastname: Yamada
authors.forename: Sari
authors.initials: S
authors.lastname: Kitahara
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Higuchi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Honda
authors.forename: Kunitoshi
authors.initials: K
authors.lastname: Kamijima
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Yamada
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage* / Q000494:pharmacology; D003511:Cyclohexanols / Q000008:administration & dosage / Q000494:pharmacology; D004565:Electroconvulsive Therapy; D020224:Expressed Sequence Tags; D005473:Fluoxetine / Q000008:administration & dosage / Q000494:pharmacology; D005625:Frontal Lobe / Q000737:chemistry*; D007099:Imipramine / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D009479:Neuropeptides / Q000032:analysis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015843:Serpins / Q000032:analysis* / Q000235:genetics; D020280:Sertraline / Q000008:administration & dosage / Q000494:pharmacology; D000069470:Venlafaxine Hydrochloride; D000097522:Neuroserpin
chemical_list: D000928:Antidepressive Agents; D003511:Cyclohexanols; D005473:Fluoxetine; D007099:Imipramine; D009479:Neuropeptides; D012333:RNA, Messenger; D015843:Serpins; D020280:Sertraline; D000069470:Venlafaxine Hydrochloride; D000097522:Neuroserpin