Reoxidation of cytosolic NADPH in Kluyveromyces lactis.
Saccharomyces cerevisiae and Kluyveromyces lactis are considered to be the prototypes of two distinct metabolic models of facultatively-aerobic yeasts: Crabtree-positive/fermentative and Crabtree-negative/respiratory, respectively. Our group had previously proposed that one of the molecular keys supporting this difference lies in the mechanisms involved in the reoxidation of the NADPH produced as a consequence of the activity of the pentose phosphate pathway. It has been demonstrated that a significant part of this reoxidation is carried out in K. lactis by mitochondrial external alternative dehydrogenases which use NADPH, the enzymes of S. cerevisiae being NADH-specific. Moreover, the NADPH-dependent pathways of response to oxidative stress appear as a feasible alternative that might co-exist with direct mitochondrial reoxidation.
Tarrío
Nuria
N
Department of Molecular and Cell Biology, University of A Coruña, Campus da Zapateira, A Coruña, Spain.
Becerra
Manuel
M
Cerdán
María Esperanza
ME
González Siso
María Isabel
MI
D003600:Cytosol / Q000378:metabolism; D007716:Kluyveromyces / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D009245:NADH Dehydrogenase / Q000378:metabolism; D009249:NADP / Q000378:metabolism*; D009250:NADP Transhydrogenases / Q000378:metabolism; D009252:NADPH Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress
D009249:NADP; D009250:NADP Transhydrogenases; D009252:NADPH Dehydrogenase; D009245:NADH Dehydrogenase