[Chemiluminescent detection of E. coli O157:H7 in food based on magnetic enzyme-linked immuno method].
OBJECTIVE
To establish a new chemiluminescent assay with high sensitivity for detecting E. coli O157:H7 in food.


METHODS
The assay was established based on the alkaline phosphatase (ALP) labeled anti-E. coli O157:H7 antibody, which reacted with an excellent chemiluminescent reagent, 3(2' spiroadamantane)4 methoxy 4(3''phosphoryloxy) phenyl 1,2 dioxetane (AMPPD) buffer solution and emitted the photons.


RESULTS
The sensitivity of chemiluminescence assay was 850, and the linear range was 1000-50,000. The intra- and interassay CV values (CVs) were respectively below 15% and 20%. The correlation coefficient was 0.9807 by comparing with the conventional counting method.


CONCLUSIONS
The chemiluminescent immunoassay is more accurate than other methods in the detection of concentration of E. coli O157:H7 in samples.
Guo
Xiao-ying
XY
State Key Laboratory for Bioelectronics, Southeast University, Nanjing 210096, China.
Wang
Yong-ning
YN
Gu
Lin-gang
LG
Liu
He-ping
HP
He
Yan-feng
YF
Lu
Zu-hong
ZH
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019453:Escherichia coli O157 / Q000276:immunology / Q000302:isolation & purification*; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology / Q000592:standards; D007118:Immunoassay; D008163:Luminescent Measurements / Q000379:methods*; D012680:Sensitivity and Specificity