[Reconstructing the JAK/STATs signal pathway restored the anti-proliferative response of MHCC97 on interferon alpha].
OBJECTIVE
To elucidate the roles of JAK/STATs signal pathway on anti-proliferative effects induced by IFN-alpha in MHCC97.


METHODS
An IRF9 expression vector was transfected into MHCC97 with Dosper. The expression of IRF9, cycle regulating proteins and the forming of ISGF3 complex were detected using Western blot and EMSA, respectively. Cell proliferation and distribution were monitored using MTT and flow cytometry.


RESULTS
High expression of IRF9 restored the anti-proliferative response of MHCC97 on IFN-alpha treatment and delayed the cell transition from S phase to G2 phase induced by IFN-alpha.


CONCLUSIONS
The integrity and functions of JAK/STATs signal pathway played an important role in mediating the anti-proliferative effects of IFN-alpha in MHCC97.
Wu
Wei-zhong
WZ
Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Sun
Hui-chuan
HC
Gao
Yan-qin
YQ
Wang
Lu
L
Tang
Zhao-you
ZY
Liu
Kang-da
KD
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D050834:Interferon-Stimulated Gene Factor 3, gamma Subunit / Q000235:genetics; D016898:Interferon-alpha / Q000378:metabolism / Q000494:pharmacology*; D053612:Janus Kinases / Q000235:genetics / Q000502:physiology*; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D050791:STAT Transcription Factors / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction; D014162:Transfection
C494227:IRF9 protein, human; D050834:Interferon-Stimulated Gene Factor 3, gamma Subunit; D016898:Interferon-alpha; D050791:STAT Transcription Factors; D053612:Janus Kinases