title: Insect nicotinic acetylcholine receptors: neonicotinoid binding site specificity is usually but not always conserved with varied substituents and species.
abstract: The diversity of neonicotinoid insecticides acting as insect nicotinic acetylcholine (ACh) receptor (nAChR) agonists is illustrated by imidacloprid (IMI) with chloropyridinylmethyl (CPM) and N-nitroimine substituents, dinotefuran (DIN) with tetrahydrofurylmethyl (TFM) and N-nitroimine moieties, and acetamiprid (ACE) with CPM and N-cyanoimine groups. These three neonicotinoids are used here as radioligands to test the hypothesis that they all bind to the same site in the same way in both fruit flies (Drosophila melanogaster) and a leafhopper pest (Homalodisca coagulata): that is, neonicotinoid binding site specificity is conserved in the insect nAChRs. Multiple approaches show that [3H]IMI and [3H]ACE interact with an identical site in both species. However, although [3H]DIN binds with high affinity in both insects, its pharmacological profile in Homalodisca is surprisingly unique, with high sensitivity to some TFM-containing compounds and ACh. The TFM moiety of DIN may bind in a different orientation compared to the CPM group of IMI and ACE.
authors.affiliation: Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720-3112, USA.
authors.forename: Hideo
authors.initials: H
authors.lastname: Honda
authors.forename: Motohiro
authors.initials: M
authors.lastname: Tomizawa
authors.forename: John E
authors.initials: JE
authors.lastname: Casida
mesh_terms: D000818:Animals; D001665:Binding Sites; D004331:Drosophila melanogaster / Q000378:metabolism*; D006146:Guanidines / Q000378:metabolism; D006430:Hemiptera / Q000378:metabolism*; D007093:Imidazoles / Q000378:metabolism; D007306:Insecticides / Q000378:metabolism*; D000073943:Neonicotinoids; D009538:Nicotine / Q000819:agonists* / Q000378:metabolism; D009574:Nitro Compounds / Q000378:metabolism; D011725:Pyridines / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000378:metabolism*; D011994:Recombinant Proteins; D013045:Species Specificity; D014316:Tritium
chemical_list: D006146:Guanidines; D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D011725:Pyridines; D011978:Receptors, Nicotinic; D011994:Recombinant Proteins; D014316:Tritium; C465368:dinotefuran; C082359:imidacloprid; C464485:acetamiprid; D009538:Nicotine