[Vitamin C decreases MMP-9 synthesis induced by hydrogen peroxide in an in vitro chorioamniotic membrane model].
OBJECTIVE
To evaluate the hydrogen peroxide ability to induce the synthesis of matrix metalloproteinase-9 (MMP-9) in an in vitro chorioamniotic membrane model.


METHODS
An experimental, transversal, analytic and prospective study was made. From amniotic membranes (n = 13) of term labour pregnant women chorionic and chorioamniotic explants were prepared; these were stimulated with hydrogen peroxide [200 mcM]. A group of explants were cultured with vitamin C, 48 hours prior to the hydrogen peroxide stimulus. Twenty-four hours after hydrogen peroxide stimulus the supernatants were collected and metalloproteinase-9 production was determined by zymography methods.


RESULTS
Hydrogen peroxide induced the synthesis of metalloproteinase-9 in chorionic and chorioamniotic membranes. When chorionic and chorioamniotic membranes were cultured with vitamin C prior to the hydrogen peroxide stimulus, the MMP-9 production diminished (p < 0.05; Dunn's method).


CONCLUSIONS
Hydrogen peroxide induced the synthesis of metalloproteinase-9 in chorionic and chorioamniotic membranes. When chorionic and chorioamniotic membranes were cultured with vitamin C, 48 hours prior to peroxide hydrogen stimulus, the metalloproteinase-9 production diminished with statistical significance.
Hernández Guerrero
César Angel
CA
Departamento de Ultramicroscopía, Instituto Nacional de Perinatología, Lomas Virreyes, México, DF. hehecatlzin@yahoo.com
Vázquez Vela
María Eugenia Frigolet
ME
Herrerías Canedo
Tomás
T
Flores Herrera
Héctor
H
Meraz Cruz
Noemí
N
D001205:Ascorbic Acid / Q000494:pharmacology*; D049033:Chorioallantoic Membrane / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*
D006861:Hydrogen Peroxide; D020780:Matrix Metalloproteinase 9; D001205:Ascorbic Acid