Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation.
Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasmid/PEI complexes in CHO cells.
Ito
Tomoko
T
Department of Textile Science, Otsuma Women's University, Tokyo, Japan.
Iida-Tanaka
Naoko
N
Niidome
Takuro
T
Kawano
Takahito
T
Kubo
Koji
K
Yoshikawa
Kenichi
K
Sato
Toshinori
T
Yang
Zhihong
Z
Koyama
Yoshiyuki
Y
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D016503:Drug Delivery Systems / Q000379:methods*; D004347:Drug Interactions / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000031:analogs & derivatives*; D008297:Male; D051379:Mice; D015533:Transcriptional Activation / Q000187:drug effects* / Q000502:physiology
D006820:Hyaluronic Acid