title: Aging in legume symbiosis. A molecular view on nodule senescence in Medicago truncatula.
abstract: Rhizobia reside as symbiosomes in the infected cells of legume nodules to fix atmospheric nitrogen. The symbiotic relation is strictly controlled, lasts for some time, but eventually leads to nodule senescence. We present a comprehensive transcriptomics study to understand the onset of nodule senescence in the legume Medicago truncatula. Distinct developmental stages with characteristic gene expression were delineated during which the two symbiotic partners were degraded consecutively, marking the switch in nodule tissue status from carbon sink to general nutrient source. Cluster analysis discriminated an early expression group that harbored regulatory genes that might be primary tools to interfere with pod filling-related or stress-induced nodule senescence, ultimately causing prolonged nitrogen fixation. Interestingly, the transcriptomes of nodule and leaf senescence had a high degree of overlap, arguing for the recruitment of similar pathways.
authors.affiliation: Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B-9052 Gent, Belgium.
authors.forename: Willem
authors.initials: W
authors.lastname: Van de Velde
authors.forename: Juan Carlos Pérez
authors.initials: JC
authors.lastname: Guerra
authors.forename: Annick
authors.initials: A
authors.lastname: De Keyser
authors.forename: Riet
authors.initials: R
authors.lastname: De Rycke
authors.forename: Stéphane
authors.initials: S
authors.lastname: Rombauts
authors.forename: Nicolas
authors.initials: N
authors.lastname: Maunoury
authors.forename: Peter
authors.initials: P
authors.lastname: Mergaert
authors.forename: Eva
authors.initials: E
authors.lastname: Kondorosi
authors.forename: Marcelle
authors.initials: M
authors.lastname: Holsters
authors.forename: Sofie
authors.initials: S
authors.lastname: Goormachtig
mesh_terms: D016000:Cluster Analysis; D018076:DNA, Complementary; D007887:Fabaceae / Q000469:parasitology / Q000502:physiology*; D029902:Medicago / Q000502:physiology*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013559:Symbiosis
chemical_list: D018076:DNA, Complementary