An immunohistological study of the integration at the bone-tendon interface after reconstruction of the anterior cruciate ligament in rabbits.
We studied bone-tendon healing using immunohistochemical methods in a rabbit model. Reconstruction of the anterior cruciate ligament was undertaken using semitendinosus tendon in 20 rabbits. Immunohistochemical evaluations were performed at one, two, four and eight weeks after the operation. The expression of CD31, RAM-11, VEGF, b-FGF, S-100 protein and collagen I, II and III in the bone-tendon interface was very similar to that in the endochondral ossification. Some of the type-III collagen in the outer layer of the graft, which was deposited at a very early phase after the operation, was believed to have matured into Sharpey-like fibres. However, remodelling of the tendon grafted into the bone tunnel was significantly delayed when compared with this ossification process. To promote healing, we believe that it is necessary to accelerate remodelling of the tendon, simultaneously with the augmentation of the ossification.
Kanazawa
T
T
Department of Orthopaedic Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan. kanazawa-t@saiseikai-hp.chuo.fukuoka.jp
Soejima
T
T
Murakami
H
H
Inoue
T
T
Katouda
M
M
Nagata
K
K
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D000911:Antibodies, Monoclonal / Q000276:immunology; D016025:Bone Transplantation / Q000379:methods*; D003094:Collagen / Q000032:analysis; D004195:Disease Models, Animal; D005346:Fibroblast Growth Factors / Q000032:analysis; D007150:Immunohistochemistry / Q000379:methods; D008264:Macrophages / Q000276:immunology; D019637:Orthopedic Procedures / Q000379:methods; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D011817:Rabbits; D009418:S100 Proteins / Q000032:analysis; D013709:Tendon Transfer / Q000379:methods*; D013710:Tendons / Q000473:pathology / Q000601:surgery; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis; D014945:Wound Healing / Q000502:physiology
D000911:Antibodies, Monoclonal; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D009418:S100 Proteins; D042461:Vascular Endothelial Growth Factor A; D005346:Fibroblast Growth Factors; D003094:Collagen