title: Resampling and deconvolution of linear time-of-flight records for enhanced protein profiling.
abstract: We have developed a peak deconvolution strategy that is applicable to the full mass range of a time-of-flight (TOF) spectrum. This strategy involves resampling a spectrum to create a time series that has equal peak widths (in time) across the entire spectrum, and then using the deconvolution filters we have previously described. We use this technique to deconvolve the protein mass spectra for blood serum and cell lysates acquired on three separate TOF instruments. Following deconvolution, we resolve spectral structures consistent with expected events such as multiply charged ions, matrix adducts and post-translational protein modifications. The deconvolution procedure produces a 40% improvement in the resolution and enhanced experimental sensitivity over the full length of the linear TOF record, up to m/z 150 000. This approach is particularly appropriate for automated data analysis and peak detection in dense TOF spectra.
authors.affiliation: Departments of Physics and Applied Science, College of William and Mary, Williamsburg, VA 23187-8795, USA. dimaly@wm.edu
authors.forename: Dariya I
authors.initials: DI
authors.lastname: Malyarenko
authors.forename: William E
authors.initials: WE
authors.lastname: Cooke
authors.forename: Eugene R
authors.initials: ER
authors.lastname: Tracy
authors.forename: Richard R
authors.initials: RR
authors.lastname: Drake
authors.forename: Susanna
authors.initials: S
authors.lastname: Shin
authors.forename: O John
authors.initials: OJ
authors.lastname: Semmes
authors.forename: Maciek
authors.initials: M
authors.lastname: Sasinowski
authors.forename: Dennis M
authors.initials: DM
authors.lastname: Manos
mesh_terms: D000465:Algorithms; D016632:Apolipoprotein A-I / Q000032:analysis; D001826:Body Fluids / Q000737:chemistry; D002138:Calibration; D003627:Data Interpretation, Statistical; D019277:Entropy; D004847:Epithelial Cells / Q000737:chemistry; D006801:Humans; D017711:Nonlinear Dynamics; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry*; D044967:Serum / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods / Q000706:statistics & numerical data*
chemical_list: D016632:Apolipoprotein A-I; D011506:Proteins