title: Antibacterial, partially acetylated oligorhamnosides from Cleistopholis patens.
abstract: Three new (1-3) and five known (4-8) partially acetylated oligorhamnoside derivatives were obtained from Cleistopholis patens via high-throughput natural products chemistry procedures. The rapid structure elucidation and dereplication were performed primarily utilizing a capillary-scale NMR probe and LR-/HRESIMS spectroscopic methods. Compounds 1, 2, and 6 were found to possess significant in vitro antibacterial activity against the Gram-positive bacteria methicillin-resistant Staphylococcus aureus ATCC 33591 and S. aureus 78-13607A with MICs of < or =16 microg/mL. Furthermore, 2 and 6 were found to show significant in vitro antibacterial activity against an expanded panel of Gram-positive pathogens including either ATCC strains or well-characterized clinical isolates from the global SENTRY Antimicrobial Surveillance Program.
authors.affiliation: Lead Discovery and Rapid Structure Elucidation Group, Sequoia Sciences Inc., 11199 Sorrento Valley Road, Suite H, San Diego, CA 92121, USA. jhu@sequoiasciences.com
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Hu
authors.forename: Eliane
authors.initials: E
authors.lastname: Garo
authors.forename: Grayson W
authors.initials: GW
authors.lastname: Hough
authors.forename: Matt G
authors.initials: MG
authors.lastname: Goering
authors.forename: Mark
authors.initials: M
authors.lastname: O'Neil-Johnson
authors.forename: Gary R
authors.initials: GR
authors.lastname: Eldridge
mesh_terms: D029061:Annonaceae / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005681:Gabon; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016106:Methicillin Resistance / Q000187:drug effects; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D006027:Glycosides