Production of a cellulase in silkworm larvae using a recombinant Bombyx mori nucleopolyhedrovirus lacking the virus-encoded chitinase and cathepsin genes.
The expression efficiency of an insect-derived cellulase was assayed in silkworm larvae infected with recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) mutants lacking the virus-encoded chitinase (chiA) and/or cathepsin (v-cath) genes. Expression was increased by approx. 10% in mutants lacking chiA or v-cath and 17% in a mutant lacking both chiA and v-cath compared with that of the unmodified recombinant BmNPV. The recombinant BmNPV lacking both chiA and v-cath can therefore be used for a large-scale production of foreign proteins in silkworms.
Lee
Kwang Sik
KS
College of Natural Resources and Life Science, Dong-A University, Busan, Korea.
Je
Yeon Ho
YH
Woo
Soo Dong
SD
Sohn
Hung Dae
HD
Jin
Byung Rae
BR
D000818:Animals; D001483:Base Sequence; D001709:Biotechnology; D012831:Bombyx / Q000201:enzymology* / Q000235:genetics / Q000821:virology*; D002480:Cellulase / Q000096:biosynthesis* / Q000235:genetics; D002688:Chitinases / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D017353:Gene Deletion; D017344:Genes, Insect; D005814:Genes, Viral; D005822:Genetic Vectors; D017924:Nucleopolyhedroviruses / Q000201:enzymology / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D004274:DNA, Recombinant; D011994:Recombinant Proteins; D002688:Chitinases; C094590:chitinase ChiA; D002480:Cellulase; D003546:Cysteine Endopeptidases; C417028:V-CATH protein, Autographa californica nucleopolyhedrovirus