Biological assay challenges from compound solubility: strategies for bioassay optimization.
Compound solubility in buffers and dimethyl sulfoxide (DMSO) has emerged as an important issue. Many discovery compounds have low solubility but are potentially valuable as leads. Unfortunately, low solubility affects bioassays by causing underestimated activity, reduced HTS-hit rates, variable data, inaccurate SAR, discrepancies between enzyme and cell assays and inaccurate in vitro ADME-Tox testing. Strategies for optimizing bioassays include: considering solubility in HTS-library design; early screening for solubility; improving storage and handling of DMSO stocks; optimizing dilution protocols; and ensuring that low-solubility compounds are fully solubilized in bioassays. These approaches allow for adequate assessments of valuable pharmacophores for which solubility can be chemically optimized at a later date.
Di
Li
L
Wyeth Research, P.O. Box CN 8000, Princeton, NJ 08543-8000, USA. dil@wyeth.com
Kerns
Edward H
EH
D001681:Biological Assay / Q000379:methods; D002021:Buffers; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D013010:Sonication
D002021:Buffers; D004364:Pharmaceutical Preparations; D012997:Solvents; D004121:Dimethyl Sulfoxide