Effect of iron on the activation of the MAPK/ERK pathway in PC12 neuroblastoma cells.
Recent evidence suggests that reactive oxygen species function as second messenger molecules in normal physiological processes. For example, activation of N-Methyl-D-Aspartate receptor results in the production of ROS, which appears to be critical for synaptic plasticity, one of the cellular mechanisms that underlie learning and memory. In this work, we studied the effect of iron in the activation of MAPK/ERK pathway and on Ca2+ signaling in neuronal PC12 cells. We found that iron-dependent generation of hydroxyl radicals is likely to modulate Ca2+ signaling through RyR calcium channel activation, which, in turn, activates the MAPK/ERK pathway. These findings underline the relevance of iron in normal neuronal function.
Muñoz
Pablo
P
PhD Program in Cell, Molecular and Neural Biology, Faculty of Sciences, Universidad de Chile. pabluesmen@yahoo.com
Zavala
Gabriela
G
Castillo
Karen
K
Aguirre
Pabla
P
Hidalgo
Cecilia
C
Núñez
Marco T
MT
D000818:Animals; D015153:Blotting, Western; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects* / Q000378:metabolism; D007501:Iron / Q000494:pharmacology*; D009447:Neuroblastoma / Q000201:enzymology*; D016716:PC12 Cells / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D019837:Ryanodine Receptor Calcium Release Channel / Q000187:drug effects* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
D017382:Reactive Oxygen Species; D019837:Ryanodine Receptor Calcium Release Channel; D007501:Iron; D048049:Extracellular Signal-Regulated MAP Kinases