title: [Vitamin C decreases MMP-9 synthesis induced by hydrogen peroxide in an in vitro chorioamniotic membrane model].
abstract: OBJECTIVE
  To evaluate the hydrogen peroxide ability to induce the synthesis of matrix metalloproteinase-9 (MMP-9) in an in vitro chorioamniotic membrane model.
  
  
  METHODS
  An experimental, transversal, analytic and prospective study was made. From amniotic membranes (n = 13) of term labour pregnant women chorionic and chorioamniotic explants were prepared; these were stimulated with hydrogen peroxide [200 mcM]. A group of explants were cultured with vitamin C, 48 hours prior to the hydrogen peroxide stimulus. Twenty-four hours after hydrogen peroxide stimulus the supernatants were collected and metalloproteinase-9 production was determined by zymography methods.
  
  
  RESULTS
  Hydrogen peroxide induced the synthesis of metalloproteinase-9 in chorionic and chorioamniotic membranes. When chorionic and chorioamniotic membranes were cultured with vitamin C prior to the hydrogen peroxide stimulus, the MMP-9 production diminished (p < 0.05; Dunn's method).
  
  
  CONCLUSIONS
  Hydrogen peroxide induced the synthesis of metalloproteinase-9 in chorionic and chorioamniotic membranes. When chorionic and chorioamniotic membranes were cultured with vitamin C, 48 hours prior to peroxide hydrogen stimulus, the metalloproteinase-9 production diminished with statistical significance.
authors.affiliation: Departamento de Ultramicroscopía, Instituto Nacional de Perinatología, Lomas Virreyes, México, DF. hehecatlzin@yahoo.com
authors.forename: César Angel
authors.initials: CA
authors.lastname: Hernández Guerrero
authors.forename: María Eugenia Frigolet
authors.initials: ME
authors.lastname: Vázquez Vela
authors.forename: Tomás
authors.initials: T
authors.lastname: Herrerías Canedo
authors.forename: Héctor
authors.initials: H
authors.lastname: Flores Herrera
authors.forename: Noemí
authors.initials: N
authors.lastname: Meraz Cruz
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology*; D049033:Chorioallantoic Membrane / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*
chemical_list: D006861:Hydrogen Peroxide; D020780:Matrix Metalloproteinase 9; D001205:Ascorbic Acid