Massively parallel signature sequencing profiling of fetal human neural precursor cells.
We have examined gene expression in multipotent neural precursor cells (NPCs) derived from human fetal (f) brain tissue and compared its expression profiles with embryonic stem (ESC) cells, embryoid body cell (EBC), and astrocyte precursors using the technique of massively parallel signature sequencing (MPSS). Gene expression profiles show that fNPCs express core neural stem cells markers and share expression profiles with astrocyte precursor cells (APCs) rather than ESC or EBC. Gene expression analysis shows that fNPCs differ from other adult stem and progenitor cells in their marker expression and activation of specific functional networks such as the transforming growth factorbeta (TGFbeta) and Notch signaling pathways. In addition, our results allow us to identify novel genes expressed in fNPCs and provide a detailed profile of fNPCs.
Cai
Jingli
J
Stem Cell Biology Unit/Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA.
Shin
Soojung
S
Wright
Lynda
L
Liu
Ying
Y
Zhou
Daixing
D
Xue
Haipeng
H
Khrebtukova
Irina
I
Mattson
Mark P
MP
Svendsen
Clive N
CN
Rao
Mahendra S
MS
D000328:Adult; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D001921:Brain; D018797:Cell Cycle Proteins / Q000235:genetics; D002478:Cells, Cultured; D002874:Chromosome Mapping; D004707:Endoderm / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D005333:Fetus; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008648:Mesoderm / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D009414:Nerve Growth Factors / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000032:analysis; D015398:Signal Transduction / Q000235:genetics; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
D018797:Cell Cycle Proteins; D005904:Glial Fibrillary Acidic Protein; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins