The effects of uncoupling protein 3 haplotypes on obesity phenotypes and very low-energy diet-induced changes among overweight Korean female subjects.
Responses to energy restriction tend to vary within the population because of genetic differences. In this study, we have genotyped 6 uncoupling protein 3 (UCP-3) polymorphisms (-55C/T, Int2-143G/C, Tyr99Tyr, Int3-47G/A, Int4-498C/T, and Tyr210Tyr) among 214 overweight Korean female subjects recruited from an obesity clinic. Three major haplotypes, identified with frequencies in excess of 0.04, were constructed from 6 single nucleotide polymorphisms. Association studies were then undertaken, involving the measurement of anthropometric characteristics and body composition both before and after 1 month of a energy-restriction regimen. At baseline, haplotype 1 (ht1) [CGTACC] was associated with elevated anthropometric characteristics, including body weight, waist-hip ratio, and body mass index, as well as body components, including body fat mass and body fat-free mass. After the completion of the 1-month weight control program, which involved a very low-energy (2900 kJ/d) diet, we analyzed the outcomes according to the UCP-3 genetic polymorphisms. Among the 3 principal haplotypes, ht1 [CGTACC] was significantly associated with an increased reduction in body weight, in the codominant (P=.022), dominant (P=.016), and recessive (P=.041) models. Body mass index reduction was associated with the ht1 haplotype in a similar fashion. Among the body components, changes in body fat mass were significantly associated with ht1 [CGTACC] (P=.028), but changes in body fat-free mass were not significantly associated with the UCP-3 polymorphism.
Cha
Min Ho
MH
Department of Medical Research, Korea Institute of Oriental Medicine, Daejon 305-811, and School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, South Korea.
Shin
Hyoung Doo
HD
Kim
Kil Soo
KS
Lee
Bong Hee
BH
Yoon
Yoosik
Y
D000328:Adult; D000483:Alleles; D001823:Body Composition / Q000235:genetics* / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004038:Diet, Reducing; D002149:Energy Intake; D005260:Female; D006239:Haplotypes; D006801:Humans; D007473:Ion Channels; D007723:Korea; D024101:Mitochondrial Proteins; D009765:Obesity / Q000178:diet therapy / Q000235:genetics* / Q000503:physiopathology; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D000071247:Uncoupling Protein 3; D049629:Waist-Hip Ratio; D015431:Weight Loss / Q000235:genetics / Q000502:physiology
D002352:Carrier Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604392:UCP3 protein, human; D000071247:Uncoupling Protein 3; D004247:DNA