Modulatory effects of chitosan adipate on the T and B lymphocyte subsets in mice.
This study examined the subsets of T lymphocytes in the thymus, spleen and mesenteric lymph nodes as well as the subsets of B lymphocytes in the spleen and mesenteric lymph nodes in mice administered chitosan adipate (20 mg/kg) intraperitoneally once or four times at 24 h intervals. The results showed that chitosan adipate decreased the percentage of immature CD4+CD8+ thymic T cells and increased the percentage of mature CD4+ and CD8+ thymocytes. The most significant stimulating effect was observed after four injections. A single exposure to chitosan adipate increased the percentage of CD4+ mesenteric lymph node cells, but four injections of the drug increased the percentage of CD4+ and CD8+ mesenteric lymph node cells. Chitosan adipate had no effect on the subset of splenic T cells. In contrast, chitosan adipate administered either once or four times increased the percentage of CD19+ splenocytes but had no effect on the percentage of CD19+ mesenteric lymph node cells. Overall, chitosan adipate induces the maturation and differentiation of thymocytes, and regulates the number of B splenic cells and lymph node T cells irrespective of the number of doses.
Obmińska-Mrukowicz
Bozena
B
Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Agricultural University, Norwida 31, 50-375 Wroc aw, Poland. m.mrukowicz@triangulum.pl
Szczypka
Marianna
M
Gaweda
Bartosz
B
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000187:drug effects* / Q000378:metabolism; D048271:Chitosan / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007155:Immunologic Factors / Q000494:pharmacology; D008221:Lymphoid Tissue / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000378:metabolism
D007155:Immunologic Factors; D048271:Chitosan