A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses.
Mosquito-borne flaviviruses include several important agents of human disease and have provided striking examples of emerging infections. In this study we present the design and validation of a single tube RT-PCR assay using a pair of consensus primers for the detection of mosquito-borne flaviviruses. Sequencing of the amplicons permits the species identification. The assay was validated using RNA from the yellow fever virus vaccine strain and from representative strains of dengue viruses 1, 2, 3 and 4, West Nile virus, Kunjin virus (a clade of West Nile virus), and St. Louis encephalitis virus.
Ayers
M
M
Metabolism Research Program, Hospital for Sick Children, Toronto, Ont., Canada.
Adachi
D
D
Johnson
G
G
Andonova
M
M
Drebot
M
M
Tellier
R
R
D001483:Base Sequence; D017931:DNA Primers; D005416:Flavivirus / Q000145:classification / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014902:West Nile virus / Q000302:isolation & purification; D015005:Yellow fever virus / Q000302:isolation & purification
D017931:DNA Primers; C096041:NS5 protein, flavivirus; D017361:Viral Nonstructural Proteins