title: Development of a murine model of chronic rhinosinusitis.
abstract: OBJECTIVE
  The aim of this study was to develop a mouse model of chronic eosinophilic rhinosinusitis.
  
  
  METHODS
  Mice were sensitized to Aspergillis fumigatus (Af) extract by intraperitoneal injection. The animals subsequently received nasal challenges with Af extract 3 times per week for 12 weeks. Sinonasal complexes were studied histologically by the study otolaryngologists and pathologists to characterize the inflammatory response.
  
  
  METHODS
  Animal care facility at an academic institution.
  
  
  RESULTS
  A chronic eosinophilic inflammatory response was evoked in all study animals. Statistical analysis was performed for inflammation, secretory cell hyperplasia, mast cells, and eosinophils. There were very significant differences (P<0.0005) between control and study mice in all categories.
  
  
  CONCLUSIONS
  Prolonged nasal challenge of Af extract creates an inflammatory response in murine nasal mucosa that mimics human chronic eosinophilic rhinosinusitis.
  
  
  CONCLUSIONS
  A murine model for chronic rhinosinusitis is reported that may facilitate future investigations into disease pathophysiology.
  
  
  METHODS
  B-2.
authors.affiliation: National Naval Medical Center, and the Uniformed Services University of the Health Sciences, Bethesda, Maryland 20889-5612, USA. rwlindsay@bethesda.med.navy.mil
authors.forename: Robin
authors.initials: R
authors.lastname: Lindsay
authors.forename: Tiffani
authors.initials: T
authors.lastname: Slaughter
authors.forename: Joy
authors.initials: J
authors.lastname: Britton-Webb
authors.forename: Steven R
authors.initials: SR
authors.lastname: Mog
authors.forename: Rich
authors.initials: R
authors.lastname: Conran
authors.forename: Monica
authors.initials: M
authors.lastname: Tadros
authors.forename: Natalie
authors.initials: N
authors.lastname: Earl
authors.forename: David
authors.initials: D
authors.lastname: Fox
authors.forename: John
authors.initials: J
authors.lastname: Roberts
authors.forename: William E
authors.initials: WE
authors.lastname: Bolger
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000946:Antigens, Fungal / Q000008:administration & dosage / Q000633:toxicity; D001232:Aspergillus fumigatus / Q000276:immunology; D002908:Chronic Disease; D004195:Disease Models, Animal; D018450:Disease Progression; D004802:Eosinophilia / Q000209:etiology / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D051379:Mice; D008807:Mice, Inbred BALB C; D012220:Rhinitis / Q000209:etiology* / Q000473:pathology; D012852:Sinusitis / Q000209:etiology* / Q000473:pathology
chemical_list: D000946:Antigens, Fungal