title: Binding of antibiotic amphotericin B to lipid membranes: a 1H NMR study.
abstract: The (1)H NMR technique was applied to study binding of AmB, an antifungal drug, to lipid membranes formed with egg yolk phosphatidylcholine. The analysis of (1)H NMR spectra of liposomes, containing also cholesterol and ergosterol (at 40 mol%), shows that AmB binds preferentially to the polar headgroups. Such a binding restricts molecular motion of the choline fragment in the hydrophilic region at the surface of liposomes but increases the segmental motional freedom in the hydrophobic core. The same effects are also observed in the sterol-containing membranes, except that the effect on the hydrophobic core was exclusively observed in the membranes containing ergosterol.
authors.affiliation: Department of Physics and Biophysics, Agricultural University, Wrocław, Poland.
authors.forename: Janina
authors.initials: J
authors.lastname: Gabrielska
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gagoś
authors.forename: Jerzy
authors.initials: J
authors.lastname: Gubernator
authors.forename: Wiesław I
authors.initials: WI
authors.lastname: Gruszecki
mesh_terms: D000666:Amphotericin B / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D004875:Ergosterol / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010713:Phosphatidylcholines / Q000737:chemistry; D011522:Protons; D012996:Solutions; D055672:Static Electricity
chemical_list: D008055:Lipids; D008081:Liposomes; D010713:Phosphatidylcholines; D011522:Protons; D012996:Solutions; D000666:Amphotericin B; D004875:Ergosterol