title: Glucocorticoid impairs growth of kidney outer medulla and accelerates loop of Henle differentiation and urinary concentrating capacity in rat kidney development.
abstract: In the rat, urinary concentrating ability develops progressively during the third postnatal (P) week and nearly reaches adult level at weaning (P21) governed by a rise in circulating glucocorticoid. Elevated extracellular osmolality can lead to growth arrest of epithelial cells. We tested the hypothesis that supranormal exposure of rat pups to glucocorticoid before the endogenous surge enhances urinary concentrating ability but inhibits renomedullary cell proliferation. Proliferating-cell nuclear antigen (PCNA)-positive cells shifted from the nephrogenic zone in the first postnatal week to Tamm-Horsfall-positive thick ascending limb (TAL) cells at the corticomedullary junction at P10-14. Renal PCNA protein abundance was stable in the suckling period and decreased 10-fold after weaning. Renal PCNA protein abundance decreased in response to dexamethasone (DEXA; 100 microg x kg(-1) x day(-1), P8-12). Prolonged administration of DEXA (P1-P11) reduced selectively the area and thickness of the outer medulla and the number of PCNA-positive cells. DEXA (P8-12) increased urinary and papillary osmolality in normohydrated and water-deprived pups and led to osmotic equilibrium between interstitium and urine, whereas apoptotic and GADD153-positive cells increased in the inner medulla. TAL-associated NaCl transporters Na-K-2Cl cotransporter, Na-K-ATPase-alpha(1), Na/H exchanger type 3, and ROMK increased significantly at weaning and in response to DEXA. We conclude that a low level of circulating glucocorticoid is permissive for proliferation of Henle's loop and the outer medulla before weaning. A reduced papillary tonicity is a crucial factor for the reduced capacity to concentrate urine during postnatal kidney development. We speculate that supranormal exposure to glucocorticoid in the suckling period can alter kidney medullary structure and function permanently.
authors.affiliation: Department of Physiology and Pharmacology, University of Southern Denmark, Odense, Denmark.
authors.forename: Jane
authors.initials: J
authors.lastname: Stubbe
authors.forename: Kirsten
authors.initials: K
authors.lastname: Madsen
authors.forename: Finn Thomsen
authors.initials: FT
authors.lastname: Nielsen
authors.forename: Ole
authors.initials: O
authors.lastname: Skøtt
authors.forename: Boye L
authors.initials: BL
authors.lastname: Jensen
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D007679:Kidney Medulla / Q000187:drug effects / Q000254:growth & development*; D008138:Loop of Henle / Q000166:cytology* / Q000187:drug effects; D015735:Mifepristone / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014556:Urine / Q000502:physiology*
chemical_list: D005938:Glucocorticoids; D018809:Proliferating Cell Nuclear Antigen; D015735:Mifepristone; D003907:Dexamethasone