title: Alleviation of picryl chloride-induced delayed type hypersensitivity reaction by saponin fraction of Gleditsia sinensis.
abstract: The objective of the present study was to investigate the effects of saponin fraction from anomalous fruits of Gleditsia sinensis (SFGS) on picryl chloride-induced delayed type hypersensitivity (PC-DTH) and functions of T lymphocytes and macrophages in mice. SFGS (100, 200 mg/kg), orally administered during either sensitization stage or effector stage, produced remarkable inhibition of PC-DTH. In vitro, SFGS (1, 2, 4 microg/ml) concentration-dependently attenuated concanavalin A (Con A)-elicited mouse splenocyte proliferation and interleukin 2 (IL-2) production. At concentrations of 10 and 20 microg/ml, SFGS inhibited lipopolysaccharide (LPS)-induced production of nitric oxide and interleukin 1beta (IL-1beta) of mouse peritoneal macrophages. The findings indicate that SFGS attenuates PC-DTH in mice, which is probably mediated by preventing proliferation and differentiation of T cells during the sensitization stage and suppressing activation of macrophages during the effector stage.
authors.affiliation: Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing.
authors.forename: Li-Fei
authors.initials: LF
authors.lastname: Hou
authors.forename: Yue
authors.initials: Y
authors.lastname: Dai
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Xia
authors.forename: Zhu-Nan
authors.initials: ZN
authors.lastname: Gong
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D031501:Gleditsia / Q000737:chemistry*; D006968:Hypersensitivity, Delayed / Q000188:drug therapy*; D007202:Indicators and Reagents; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000096:biosynthesis; D010853:Picryl Chloride / Q000009:adverse effects*; D012503:Saponins / Q000627:therapeutic use*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D007202:Indicators and Reagents; D007375:Interleukin-1; D007376:Interleukin-2; D008070:Lipopolysaccharides; D012503:Saponins; D003208:Concanavalin A; D009569:Nitric Oxide; D010853:Picryl Chloride