Selenium speciation by high-performance anion-exchange chromatography-post-column UV irradiation coupled with atomic fluorescence spectrometry.
A technique for the speciation of selenomethylcysteine (SeMeCys), selenocystine (SeCys), selenite [Se(IV)] and selenomethionine (SeMet) was established in this paper using high-performance anion-exchange chromatography coupled with atomic fluorescence spectrometry (HPAEC-AFS). Analytes were separated on an AminoPac PA10 column and then digested by on-line ultraviolet (UV) irradiation, which destroyed organic compound structure. Hydride generation was used as an available sample introduction technique for atomic fluorescence detection. The detection limits of four compounds were 1-5 microg/L (250 microL injection, 10 times of the baseline noise). The relative standard deviations (RSDs), calculated from seven consecutive injections of 100 microg/L standard mixtures, were from 2 to 4%. Selenious yeast tablet, which had been proposed as selenium supplement, and human urine collected from a volunteer were analyzed. Good spiked recoveries from 86 to 103% were obtained.
Liang
Lina
L
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
Mo
Shumin
S
Zhang
Ping
P
Cai
Yaqi
Y
Mou
Shifen
S
Jiang
Guibin
G
Wen
Meijuan
M
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003553:Cystine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D007475:Ion Exchange Resins / Q000737:chemistry; D016566:Organoselenium Compounds / Q000032:analysis / Q000652:urine; D015203:Reproducibility of Results; D012643:Selenium / Q000032:analysis* / Q000652:urine; D012645:Selenomethionine / Q000032:analysis / Q000652:urine; D012972:Sodium Hydroxide / Q000737:chemistry; D018038:Sodium Selenite / Q000032:analysis / Q000652:urine; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods*; D013607:Tablets / Q000737:chemistry; D014466:Ultraviolet Rays; D015003:Yeasts / Q000737:chemistry
D007475:Ion Exchange Resins; D016566:Organoselenium Compounds; D013607:Tablets; C009226:selenocystine; D003553:Cystine; D012972:Sodium Hydroxide; D012645:Selenomethionine; D012643:Selenium; D018038:Sodium Selenite; D006851:Hydrochloric Acid