Distinct DNA repair pathways involving RecA and nonhomologous end joining in Mycobacterium smegmatis.
Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ (ku and ligD) was tested on the ability of bacteria to join breaks in plasmids transformed into them and in their response to chemicals that damage DNA. The results provide in vivo evidence that only NHEJ is required for the repair of noncompatible DNA ends. By contrast, the response of mycobacteria to mitomycin C preferentially involved a RecA-dependent pathway.
Korycka-Machala
Malgorzata
M
Centre of Medical Biology, Polish Academy of Sciences, Lodowa, Poland.
Brzostek
Anna
A
Rozalska
Sylwia
S
Rumijowska-Galewicz
Anna
A
Dziedzic
Renata
R
Bowater
Richard
R
Dziadek
Jaroslaw
J
D004249:DNA Damage; D011088:DNA Ligases / Q000502:physiology*; D004260:DNA Repair; D016685:Mitomycin / Q000494:pharmacology; D020102:Mycobacterium smegmatis / Q000235:genetics*; D011938:Rec A Recombinases / Q000502:physiology*; D011995:Recombination, Genetic
D016685:Mitomycin; D011938:Rec A Recombinases; D011088:DNA Ligases