An ionic polymer bead-supported lipid system.
In this paper, we report on the preparation of an ionic polymer bead-supported lipid system several hundred micrometers in diameter. The electrostatic attractive interactions between anionic lipids and cationic polymer beads served as a "molecular glue" to immobilize the lipids on the beads, and then the immobilized lipids prompted the spontaneous formation of lipid bilayer membranes. Confocal fluorescence microscopic techniques revealed that the lipid bilayer membranes were located along the outline of the beads. In addition, the integrity of the lipid bilayer membranes was microscopically confirmed by a low-molecular-weight dye (trypan blue) exclusion test.
Haratake
M
M
Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521, Japan. haratake@nagasaki-u.ac.jp
Hidaka
S
S
Ono
M
M
Nakayama
M
M
D003599:Cytoskeleton / Q000737:chemistry; D007202:Indicators and Reagents; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry; D018613:Microscopy, Confocal; D008958:Models, Molecular; D008968:Molecular Conformation; D015388:Organelles / Q000502:physiology; D014343:Trypan Blue / Q000737:chemistry
D007202:Indicators and Reagents; D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids; D008565:Membrane Proteins; D014343:Trypan Blue