Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy.
The signal and idler beams from a picosecond, synchronously pumped optical parametric oscillator (OPO) provide the two colors necessary for coherent anti-Stokes Raman scattering (CARS) microscopy. The OPO provides a continuously tunable frequency difference between the two beams over a broad range of Raman shifts (100-3700 cm(-1)) by varying the temperature of a single nonlinear crystal. The near-infrared output (900-1300 nm) allows for deep penetration into thick samples and reduced nonlinear photodamage. Applications of this light source to in vivo cell and ex vivo tissue imaging are demonstrated.
Ganikhanov
Feruz
F
Department of Chemistry and Chemical Biology, Harvard University, Massachusetts 02138, USA.
Carrasco
Silvia
S
Sunney Xie
X
X
Katz
Mordechai
M
Seitz
Wolfgang
W
Kopf
Daniel
D
D000818:Animals; D004431:Ear, External / Q000166:cytology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007834:Lasers; D008029:Lighting / Q000295:instrumentation*; D051379:Mice; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods