[Gene transfer of CD151 enhanced myocardial angiogenesis and improved cardiac function in rats with experimental myocardial infarction].
OBJECTIVE
To investigate the effects of in vivo CD151 gene transfer on angiogenesis and heart function in rats with myocardial infarction.


METHODS
Acute myocardial infarction (AMI) was induced in male Sprague-Dawley (SD) rats by left anterior descending coronary artery ligation. The surviving rats randomly received myocardial injection of saline (MI control), pAAV-CD151 and pAAV-GFP (n = 12/group). Sham-operated rats without myocardial injection (n = 12) were taken as normal control. Four weeks later, heart function and the expression of CD151 were measured. Micro vessels density (MVD) in infarct myocardium was observed by factor VIII related antigen immunochemical staining.


RESULTS
The expression of CD151 (1.98 +/- 0.23 vs. 0.91 +/- 0.09, P < 0.01) and MVD counting [(385.4 +/- 79.9) vs. (252.5 +/- 43.0) n/mm(2), P < 0.01] in pAAV-CD151 treated MI rats were significantly higher than that in MI control group, similarly, EF (64.0 +/- 8.7)% vs. (41.5 +/- 5.0)%, P < 0.01] and dp/dt(max) (6620.2 +/- 884.6 vs. 5545.5 +/- 693.0, P < 0.01) were also significantly increased post pAAV-CD151 treatment. These parameters were not affected by pAAV-GFP treatment.


CONCLUSIONS
CD151 in vivo gene transfer for rats with acute myocardial infarction enhanced myocardial angiogenesis and improved left ventricular function.
Wang
Li
L
Department of Cardiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Yang
Jun
J
Liu
Zheng-xiang
ZX
Chen
Bin
B
Liu
Jian
J
Lan
Rong-fang
RF
Qin
Jin
J
D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D004195:Disease Models, Animal; D015316:Genetic Therapy; D005822:Genetic Vectors; D008297:Male; D009203:Myocardial Infarction / Q000503:physiopathology / Q000628:therapy*; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D060185:Tetraspanin 24; D014162:Transfection; D016277:Ventricular Function, Left
D015703:Antigens, CD; D060185:Tetraspanin 24