Effect of Fasciola gigantica excretory secretory antigen on rat hematological indices.
The present study was undertaken to investigate the effect of Fasciola gigantica excretory secretory antigen (Fg-ESA) on rat hematological indices. Fg-ESA was prepared by keeping thoroughly washed 40 F. gigantica flukes in 100 ml phosphate buffer saline (PBS) for 2 h at 37 degrees C, and centrifuging the supernatant at 12,000 g at 4 degrees C for 30 min. The protein content of Fg-ESA was adjusted to 1.8 mg/ml. The rats were randomly divided into two groups of six rats each. Rats in group A received 0.5 ml of Fg-ESA intraperitoneally (i.p.) for 7 days, whereas control rats in group B received 0.5 ml of PBS i.p. for 7 days. Hemograms of both groups were studied initially and on days 0, 2, 4, 14 and 21 after the final injection of Fg-ESA or PBS. Progressive and significant (p < 0.01) declines in the values of hemoglobin, hematocrit, and total erythrocyte count were observed without significant (p > 0.05) changes in the values of mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, or mean corpuscular volume in group A. Thus, we conclude that Fg-ESA induces normocytic normochromic anemia in rats.
Ganga
G
G
Clinical Medicine Laboratory, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122 (U.P.), India.
Varshney
J P
JP
Sharma
R L
RL
D000818:Animals; D000947:Antigens, Helminth / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005209:Fasciola / Q000378:metabolism*; D005260:Female; D015801:Helminth Proteins / Q000494:pharmacology*; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D008297:Male; D051381:Rats; D013997:Time Factors
D000947:Antigens, Helminth; D015801:Helminth Proteins; D006454:Hemoglobins