Factors derived from mouse embryonic stem cells promote self-renewal of goat embryonic stem-like cells.
Goat embryonic stem (ES)-like cells could be isolated from primary materials-inner cell masses (ICMs) and remain undifferentiated for eight passages in a new culture system containing mouse ES cell conditioned medium (ESCCM) and on a feeder layer of mouse embryo fibroblasts (MEFs). However, when cultured in medium without mouse ESCCM, goat ES-like cells could not survive for more than three passages. In addition, no ES-like cells could be obtained when ICMs were cultured on goat embryo fibroblasts or the primary materials-whole goat blastocysts were cultured on MEFs. Goat ES-like cells isolated from ICMs had a normal karyotype and highly expressed alkaline phosphatase. Multiple differentiation potency of the ES-like cells was confirmed by differentiation into neural cells and fibroblast-like cells in vitro. These results suggest that mouse ES cells might secrete factors playing important roles in promoting goat ES-like cells' self-renewal, moreover, the feeder layers and primary materials could also influence the successful isolation of goat ES-like cells.
Tian
Hai-Bin
HB
Shanghai Transgenic Research Center, Shanghai 201203, China.
Wang
Hong
H
Sha
Hong-Ying
HY
Xu
Xu-Jun
XJ
Zhu
Min
M
Wu
You-Bing
YB
Cheng
Sheng-Hua
SH
Chen
Jian-Quan
JQ
Shi
Yong-Xiang
YX
Bai
Zeng-Liang
ZL
Cheng
Guo-Xiang
GX
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D017077:Culture Media, Conditioned; D004622:Embryo, Mammalian / Q000166:cytology; D006041:Goats / Q000196:embryology*; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D017077:Culture Media, Conditioned