title: Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives.
abstract: Mild acids are known to activate dentin matrix metalloproteinase (MMPs). All self-etching dental adhesives are acidic (pH 1.5-2.7) and may activate dentin MMPs. The purpose of this study was to compare the ability of several all-in-one adhesives to activate gelatinolytic and collagenolytic activities in powdered mineralized dentin. Powdered dentin made from human teeth was mixed with all-in-one adhesives (Clearfil Tri-S Bond, G-Bond, Adper Prompt L-Pop) or a self-etching primer (Clearfil SE Bond primer) for varying times and then the reaction was stopped by extracting the adhesives using acetone. Fresh untreated mineralized dentin powder had a gelatinolytic activity of 3.31 +/- 0.39 relative fluorescent units (RFU) per mg dry weight (24 h) that increased, over storage time, to 87.5 RFU mg(-1) (24 h) after 6-8 wk. When fresh powder was treated with acidic Tri-S Bond, the gelatinolytic activity increased from 3.24 +/- 0.70 RFU mg(-1) to > 112.5 RFU mg(-1) (24 h) after 20 min and then remained unchanged. Monomers with lower pH values produced less activity. There was a significant, direct correlation between gelatinolytic activity and pH, with Tri-S giving the highest activity. Coating dentin powder with Tri-S resin prevented fluorescent substrates from gaining access to the enzyme, even though it activated the enzyme. In conclusion, self-etch adhesives may activate latent MMP and increase the activity to near-maximum levels and contribute to the degradation of resin-dentin bonds over time.
authors.affiliation: Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishitani
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshiyama
authors.forename: Bakul
authors.initials: B
authors.lastname: Wadgaonkar
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Breschi
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Mannello
authors.forename: Annalisa
authors.initials: A
authors.lastname: Mazzoni
authors.forename: Ricardo M
authors.initials: RM
authors.lastname: Carvalho
authors.forename: Leo
authors.initials: L
authors.lastname: Tjäderhane
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Tay
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
mesh_terms: D000269:Adhesives / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D017364:Collagenases / Q000187:drug effects*; D003804:Dentin / Q000201:enzymology*; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D018093:Gelatinases / Q000187:drug effects*; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D008689:Methacrylates / Q000494:pharmacology; D010756:Phosphoric Acids / Q000494:pharmacology; D019279:Resin Cements / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000269:Adhesives; C029849:Clearfil Bonding Agent; C438313:Clearfil SE Bond; D017220:Dentin-Bonding Agents; C498353:G-Bond; D008689:Methacrylates; D010756:Phosphoric Acids; C408584:Prompt L-Pop; D019279:Resin Cements; D019343:Citric Acid; C030242:phosphoric acid; D017364:Collagenases; D018093:Gelatinases; D006851:Hydrochloric Acid