title: In vivo echocardiographic imaging of transplanted human adult stem cells in the myocardium labeled with clinically applicable CliniMACS nanoparticles.
abstract: OBJECTIVE
  Intramyocardial transplantation of bone marrow-derived cells is currently under clinical evaluation as a therapy of heart failure. A major limitation of all clinical studies dealing with myocardial cell engraftment is the inability to track the fate of the transplanted cells. We present a clinically applicable technique using transesophageal echocardiography (TEE) of CliniMACS nanoparticle labeled transplanted CD133+ cells in ischemic hearts.
  
  
  RESULTS
  CD133+ cells were isolated from human bone marrow by CliniMACS magnetic bead selection. Positive (5 x 10(6) cells) cells were transplanted into porcine ischemic myocardium (n = 6). Control animals (n = 5) received medium injection. TEE was performed to demonstrate the distribution of the cells during and 8 weeks after the procedure. Accumulations of labeled CD133+ cells of adjacent injections in the myocardium were discriminatively identified by TEE.
  
  
  CONCLUSIONS
  TEE is an elegant method for real-time documentation of successful transplantation and cell colony localization of magnetically labeled CD133+ cells in the ischemic myocardium. Our method should be of special interest for TEE-guided transcatheter injection of cells.
authors.affiliation: Division of Thoracic- and Cardiovascular Surgery, Hannover Medical School, Hannover, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Bara
authors.forename: Alireza
authors.initials: A
authors.lastname: Ghodsizad
authors.forename: Michael
authors.initials: M
authors.lastname: Niehaus
authors.forename: Mahyar
authors.initials: M
authors.lastname: Makoui
authors.forename: Christoph
authors.initials: C
authors.lastname: Piechaczek
authors.forename: Ulrich
authors.initials: U
authors.lastname: Martin
authors.forename: Gregor
authors.initials: G
authors.lastname: Warnecke
authors.forename: Matthias
authors.initials: M
authors.lastname: Karck
authors.forename: Emmeran
authors.initials: E
authors.lastname: Gams
authors.forename: Hans Michael
authors.initials: HM
authors.lastname: Klein
authors.forename: Axel
authors.initials: A
authors.lastname: Haverich
authors.forename: Arjang
authors.initials: A
authors.lastname: Ruhparwar
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods; D002478:Cells, Cultured; D003287:Contrast Media; D004452:Echocardiography / Q000379:methods*; D017548:Echocardiography, Transesophageal / Q000379:methods*; D005240:Feasibility Studies; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D018189:Immunomagnetic Separation / Q000379:methods; D009203:Myocardial Infarction / Q000000981:diagnostic imaging* / Q000601:surgery*; D009206:Myocardium; D049329:Nanostructures / Q000032:analysis; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000000981:diagnostic imaging*; D013552:Swine
chemical_list: D003287:Contrast Media