Role of the Cys18-Cys274 disulfide bond and of the third extracellular loop in the constitutive activation and internalization of angiotensin II type 1 receptor.
An insertion of residues in the third extracellular loop and a disulfide bond linking this loop to the N-terminal domain were identified in a structural model of a G-protein coupled receptor specific to angiotensin II (AT1 receptor), built in homology to the seven-transmembrane-helix bundle of rhodopsin. Both the insertion and the disulfide bond were located close to an extracellular locus, flanked by the second extracellular loop (EC-2), the third extracellular loop (EC-3) and the N-terminal domain of the receptor; they contained residues identified by mutagenesis studies to bind the angiotensin II N-terminal segment (residues D1 and R2). It was postulated that the insertion and the disulfide bond, also found in other receptors such as those for bradykinin, endothelin, purine and other ligands, might play a role in regulating the function of the AT1 receptor. This possibility was investigated by assaying AT1 forms devoid of the insertion and with mutations to Ser on both positions of Cys residues forming the disulfide bond. Binding and activation experiments showed that abolition of this bond led to constitutive activation, decay of agonist binding and receptor activation levels. Furthermore, the receptors thus mutated were translocated to cytosolic environments including those in the nucleus. The receptor form with full deletion of the EC-3 loop residue insertion, displayed a wild type receptor behavior.
Correa
Silvana A A
SA
Department of Biophysics, Universidade Federal de São Paulo-Escola Paulista de Medicina, Rua Botucatu 862, 04023-062, São Paulo, SP, Brazil.
Pignatari
Graciela C
GC
Ferro
Emer S
ES
Pacheco
Nelson A S
NA
Costa-Neto
Claudio M
CM
Pesquero
João B
JB
Oliveira
Laerte
L
Paiva
Antonio C M
AC
Shimuta
Suma I
SI
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001896:Boron Compounds; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003553:Cystine / Q000502:physiology*; D004220:Disulfides / Q000378:metabolism*; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D007295:Inositol Phosphates / Q000096:biosynthesis; D018613:Microscopy, Confocal; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D044140:Receptor, Angiotensin, Type 1 / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004220:Disulfides; D007295:Inositol Phosphates; D044140:Receptor, Angiotensin, Type 1; D011993:Recombinant Fusion Proteins; D000804:Angiotensin II; D049452:Green Fluorescent Proteins; D003553:Cystine