title: [Synthesis of modified fragments of fibrinogen and their effect on the activity of proteolytic enzymes].
abstract: New analogues of the Gly-Pro-Arg and Arg-Gly-Asp fragments of fibrinogen were synthesized: Gly-Pro-Arg-Pro (I), Gly-Pro-Arg-Pro-Met-OMe (II), Gly-Pro-Arg-Pro-Phe (III), Gly-Pro-Arg-Pro-Asp (IV), Gly-Pro-Arg-Pro-Glu (V), and Arg-Asn-Trp-Asp (VI). Their effect on the activity of proteases of various types was studied with the method of lysis of fibrin plates. All the peptides were found to inhibit plasmin activity (by 60-85%) and the gamma-subunit of nerve growth factor (by 55-93%). Tetrapeptide (VI) proved to be an effective inhibitor of tissue activator of plasminogen and the gamma-subunit of nerve growth factor (by 96 and 93%, respectively). The peptides exerted practically no effect on the activity of urokinase and moderately inhibited the activity of streptokinase [(III), (IV), and (VI)], papain [(I), (II), (IV), and (VI)], subtilisin [(V) and (VI)], alpha-chymotrypsin [(III), (V), and VI)], and Bacillus subtilis metalloprotease (VI). They inhibit trypsin [except for (I) and (III)] when applied on fibrin plates at a concentration of 1 x 10(-2) M, while, at a concentration of 1 x 10(-3) M, (I) and (II) induced an increase in proteolytic activity by 35 and 47%, respectively.
authors.forename: V N
authors.initials: VN
authors.lastname: Nikandrov
authors.forename: N S
authors.initials: NS
authors.lastname: Pyzhova
authors.forename: V P
authors.initials: VP
authors.lastname: Golubovich
authors.forename: O V
authors.initials: OV
authors.lastname: Mel'nik
authors.forename: V P
authors.initials: VP
authors.lastname: Martinovich
mesh_terms: D000818:Animals; D020536:Enzyme Activators / Q000138:chemical synthesis / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry*; D011480:Protease Inhibitors / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D020536:Enzyme Activators; D010446:Peptide Fragments; D011480:Protease Inhibitors; D005340:Fibrinogen