title: Characterization of Short Time Labeled Adenosine Monophosphate-rich Ribonucleic Acids of Soybean.
abstract: The total population of newly synthesized (32)P-AMP-rich RNA has been separated into two major types based on repeated fractionation on methylated albumin-kieselguhr columns. The purified D-RNA which elutes, under our experimental conditions, primarily in the salt gradient has a GMP/AMP ratio of about 0.8 and an AMP + UMP content of about 56 mole per cent. The purified TB-RNA which preferentially remains bound to the column in the salt gradient has a GMP/AMP ratio of about 0.4 to 0.45 and an AMP + UMP content of about 65 mole per cent. In addition to being distinguished by their fractionation on the methylated albumin-kieselguhr column and base composition analysis, purified D-RNA and TB-RNA have different size distributions on sucrose gradient and acrylamide gel fractionation, are differentially associated with polyribosomes and have different stabilities in the tissue.
authors.affiliation: Department of Botany, University of Georgia, Athens, Georgia 30601.
authors.forename: J L
authors.initials: JL
authors.lastname: Key
authors.forename: C J
authors.initials: CJ
authors.lastname: Leaver
authors.forename: J R
authors.initials: JR
authors.lastname: Cowles
authors.forename: J M
authors.initials: JM
authors.lastname: Anderson