title: A plant miRNA contributes to antibacterial resistance by repressing auxin signaling.
abstract: Plants and animals activate defenses after perceiving pathogen-associated molecular patterns (PAMPs) such as bacterial flagellin. In Arabidopsis, perception of flagellin increases resistance to the bacterium Pseudomonas syringae, although the molecular mechanisms involved remain elusive. Here, we show that a flagellin-derived peptide induces a plant microRNA (miRNA) that negatively regulates messenger RNAs for the F-box auxin receptors TIR1, AFB2, and AFB3. Repression of auxin signaling restricts P. syringae growth, implicating auxin in disease susceptibility and miRNA-mediated suppression of auxin signaling in resistance.
authors.affiliation: Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
authors.forename: Lionel
authors.initials: L
authors.lastname: Navarro
authors.forename: Patrice
authors.initials: P
authors.lastname: Dunoyer
authors.forename: Florence
authors.initials: F
authors.lastname: Jay
authors.forename: Benedict
authors.initials: B
authors.lastname: Arnold
authors.forename: Nihal
authors.initials: N
authors.lastname: Dharmasiri
authors.forename: Mark
authors.initials: M
authors.lastname: Estelle
authors.forename: Olivier
authors.initials: O
authors.lastname: Voinnet
authors.forename: Jonathan D G
authors.initials: JD
authors.lastname: Jones
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism; D005408:Flagellin / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D035683:MicroRNAs / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000254:growth & development / Q000472:pathogenicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000502:physiology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014170:Transformation, Genetic
chemical_list: C000596911:AFB3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D044783:F-Box Proteins; D007210:Indoleacetic Acids; D035683:MicroRNAs; D012333:RNA, Messenger; D018749:RNA, Plant; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C501405:TIR1 protein, Arabidopsis; D005408:Flagellin