title: Temporal dynamics of effect concentrations.
abstract: In effect assessment the comparability and applicability of LCx and ECx values, which are calculated at single points in time during exposure, relies on the ability to perform a valid extrapolation to other time points of interest. The behavior of LCx in time has been extensively studied, and the behavior of ECx in time is expected to follow similar dynamics, as it is considered that the LCx is just a specific case of ECxs. However, most models have focused on validating the dynamics of LCx, and hardly anything is known about the time dependence of ECx for other endpoints or whether it is comparable to that of LCxs. We have created four scenarios where we study the dynamics of the ECx for different endpoints and how it is affected by the characteristics of two different compounds (carbendazim and pentachlorobenzene) and of two different life history strategies (hermaphroditic and sexually reproducing strains of Caenorhabditis elegans). The observed patterns of behavior in time of the ECx for body size and for reproduction showed unexpected dynamics that deviate considerably from that of the LCx. It was demonstrated that the temporal dynamics of ECx were very different for each particular endpoint. The shape of the ECx-time curves depends on the intrinsic characteristics of the endpoint of study, as well as on the characteristics of the compound and life history strategy of the organism. This makes extrapolation in time or between endpoints difficult and hampers the comparability of results based on this summary statistic. The interpretation of the results from toxicity tests can be improved through process-based modeling, as demonstrated on the current data set.
authors.affiliation: Laboratory of Nematology, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands. Olga.Alda@wur.nl
authors.forename: Olga Alda
authors.initials: OA
authors.lastname: Alvarez
authors.forename: Tjalling
authors.initials: T
authors.lastname: Jager
authors.forename: Beatriz Nuñez
authors.initials: BN
authors.lastname: Colao
authors.forename: Jan E
authors.initials: JE
authors.lastname: Kammenga
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000502:physiology; D008962:Models, Theoretical; D012098:Reproduction / Q000187:drug effects