title: [Antibodies against the chlamydial heat shock protein in women with periadnexal adhesions].
abstract: OBJECTIVE
  To compare and analyze IgG antibodies against chlamydial heat shock protein (cHSP60) in the blood serum of women with or without periadnexal adhesions in small pelvis.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Department of Gynaecology and Obstetrics, Masaryk University, Brno, Veterinary Research Institute, Brno, Department of Clinical Microbiology, Faculty Hospital Brno.
  
  
  METHODS
  IgG antibodies against cHSP60 in the blood serum were detected by means of recombinant enzyme immunoanalysis ("cHSP60 - IgG - rELISA medac"). The presence of antibodies was expressed as +, ++ or +++ pozitive. Laparoscopy according to a standard procedure was performed in 76 women (age 28 +/- 43 years) from couples with fertility disorders treated in the Centre of Reproductive Medicine, Department of Gynaecology and Obstetrics. Chí-square test (programme Statcalc - EpiInfo 6) and calculation of OR value were used for statistical analysis.
  
  
  RESULTS
  Twenty-four women (31.6%) had normal finding in small pelvis. Periadnexal adhesions were detected in 43 (56.6%) patients, hydrosalpinx in 13 cases (17.1%), endometriosis in 27 (35.5%) patients and uterine myomas in 11 (145%) women. In the group of 33 women without periadnexal adhesions cHSP-60 antibodies were pozitive in 3 women (9.1%), in 43 women with periadnexal adhesions were pozitive in 17 cases (39.5%). In 13 patients with hydrosalpinx cHSP-60 antibodies were pozitive in 61.5%. In patients with periadnexal adhesions semiquantitative pozitivity +++ was detected in 8 cases (18.6%), 4 of them with hydrosalpinx (30.8%). Statistical analysis found significantly higher presence of cHSP-60 antibodies in women with periadnexal adhesions (OR = 654, CI interval = 1.59 - 37.81, chi-square: p = 0.0028)
  
  
  CONCLUSIONS
  Women with periadnexal adhesions have more frequent and stronger pozitivity of IgG antibodies against chlamydial heat shock protein cHSP60.
authors.affiliation: Gynekologicko-porodnická klinika Masarykovy univerzity a FN Brno.
authors.forename: I
authors.initials: I
authors.lastname: Crha
authors.forename: L
authors.initials: L
authors.lastname: Pospísil
authors.forename: H
authors.initials: H
authors.lastname: Stroblová
authors.forename: P
authors.initials: P
authors.lastname: Ventruba
authors.forename: J
authors.initials: J
authors.lastname: Záková
authors.forename: M
authors.initials: M
authors.lastname: Huser
mesh_terms: D000291:Adnexal Diseases / Q000276:immunology*; D000328:Adult; D018834:Chaperonin 60 / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D000267:Tissue Adhesions
chemical_list: D018834:Chaperonin 60; D007074:Immunoglobulin G