Spectroscopic spectral-domain optical coherence microscopy.
The spectroscopic content within optical coherence tomography (OCT) data can provide a wealth of information. Spectroscopic OCT methods are frequently limited by time-frequency trade-offs that limit high spectral and spatial resolution simultaneously. We present spectroscopic spectral-domain optical coherence microscopy performed with a multimodality microscope. Restricting the spatial extent of the signal by using high-numerical-aperture optics makes high-resolution spectroscopic information accessible, facilitated with spectral-domain detection. Simultaneous acquisition of multiphoton microscopy images is used to validate tissue structure and localization of nuclei within individual cells.
Xu
Chengyang
C
Biophotonics Imaging Laboratory, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, Illinois 61801, USA.
Vinegoni
Claudio
C
Ralston
Tyler S
TS
Luo
Wei
W
Tan
Wei
W
Boppart
Stephen A
SA
D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D051381:Rats; D013057:Spectrum Analysis / Q000379:methods*; D041623:Tomography, Optical Coherence / Q000379:methods*