title: Expression of thick filament proteins during ontogenesis of the mussel Mytilus trossulus (Mollusca: Bivalvia).
abstract: The appearance of thick filament proteins organized into supramolecular complexes was studied by SDS-PAGE and Western-blot analysis at different developmental stages of the mussel Mytilus trossulus. Paramyosin appeared at the egg stage, while twitchin and myorod appeared at the blastula stage (12 h after fertilization). In addition, RT-PCR analysis showed that the twitchin genes were expressed starting from the blastula stage. Thus, the proteins forming thick filaments of the contractile apparatus of mussel muscles are expressed long before the formation of the first well-organized muscle system of the veliger larvae (55 h). Further, the ratios actin/myosin heavy chain (MHC) and paramyosin/MHC at the veliger stage (96 h) distinctly differed from those in the adult mussel.
authors.affiliation: Department of Cell Biophysics, Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690041, Russia. nelodin@mail.ru
authors.forename: N
authors.initials: N
authors.lastname: Odintsova
authors.forename: V
authors.initials: V
authors.lastname: Dyachuk
authors.forename: K
authors.initials: K
authors.lastname: Kiselev
authors.forename: N
authors.initials: N
authors.lastname: Shelud'ko
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian; D015870:Gene Expression; D008018:Life Cycle Stages; D009218:Myosins / Q000378:metabolism*; D049878:Mytilus / Q000196:embryology* / Q000254:growth & development*; D013995:Time; D014335:Tropomyosin / Q000378:metabolism*
chemical_list: D014335:Tropomyosin; D009218:Myosins