Thermodynamic equilibrium of domains in a two-component Langmuir monolayer.
This [corrected] article outlines the results from a combined experimental and theoretical study on the properties of circular domains in a mixed Langmuir monolayer at thermodynamic equilibrium. The mixed monolayer consisted of a binary mixture of dimyristoyl-phosphatidyl-choline and dihydrocholesterol. A long-term fluorescence microscopy study of these domains was carried out over the course of approximately 60 h. Image analysis of the domains over time revealed that the domains ripened slowly with an [corrected] increase in mean domain radius and a [corrected] decrease in domain number density. At the end of the measurement, the domains remained polydisperse, and true thermodynamic equilibrium was not reached. Theoretically, collective thermodynamic equilibrium properties such as mean domain size and size distribution were calculated by combining micelle self-assembly theory and the "equivalent dipole" model for the self-energy of two-dimensional domains. The calculations predicted existence of finite-sized circular domains at equilibrium. This suggests that equilibrium circular monolayer domains of single- or multicomponent lipids with a finite size distribution should form only at very limited experimental conditions. Both the predicted mean domain size and size distribution are strongly affected by line tension and dipole moment density difference. A comparison between the theoretical and experimental results is made.
Hu
Yufang
Y
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California, USA. yufang_hu@hotmail.com
Meleson
Kieche
K
Israelachvili
Jacob
J
D004083:Cholestanol / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D008560:Membrane Fluidity; D008567:Membranes, Artificial; D008823:Micelles; D008856:Microscopy, Fluorescence; D008962:Models, Theoretical; D013499:Surface Properties; D013816:Thermodynamics
D008567:Membranes, Artificial; D008823:Micelles; D004083:Cholestanol; D004134:Dimyristoylphosphatidylcholine