The photochemistry of 5-BrdCpdC, dCp5-BrdCpdA, and dCp5-BrdCdT in aqueous solution.
We isolated an intrastrand crosslink product from the Pyrex-filtered UV light irradiation of 5-BrdCpdC in aqueous solution. ESI-MS, MS/MS, and 2-D nuclear Overhauser effect spectroscopy (NOESY) results showed that the C5 carbon atoms of the two cytosines are covalently bonded. The same cross-link product can also be induced from the similar UV irradiation of dCp5-BrdCpdA and dCp5-BrdCpdT.
Gao
Yuan
Y
Department of Chemistry-027, University of California, 900 University Avenue, Riverside, CA 92521, USA. yinsheng.wang@ucr.edu
Wang
Yinsheng
Y
D002851:Chromatography, High Pressure Liquid; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010777:Photochemistry; D021241:Spectrometry, Mass, Electrospray Ionization
C065702:5-bromodeoxycytosine; D003596:Cytosine