Propionibacterium acnes DNA from an explanted intraocular lens detected by polymerase chain reaction in a case of chronic pseudophakic endophthalmitis.
We report a case of Propionibacterium acnes DNA from the explanted intraocular lens (IOL) a 76-year-old man with delayed-onset chronic endophthalmitis. The detection was by polymerase chain reaction (PCR). Slitlamp microscopic examination showed cells and flare in the anterior chamber, keratic precipitates on the endothelium, and multiple white plaques on both surfaces of the IOL. Propionibacterium acnes DNA was found in both the aqueous humor and the explanted IOL by PCR. Scanning electron microscopy showed diphtheroid particles, a coating of biofilm-like deposits, as well as scattered crystals on the surface of the explanted IOL and supported the possibility of P acne colonies on the IOL.
Lai
Jui-Yang
JY
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, China.
Chen
Ko-Hua
KH
Lin
Ying-Cheng
YC
Hsu
Wen-Ming
WM
Lee
Shui-Mei
SM
D000368:Aged; D001082:Aqueous Humor / Q000382:microbiology; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis*; D020878:Device Removal; D009877:Endophthalmitis / Q000382:microbiology* / Q000601:surgery; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology* / Q000601:surgery; D006801:Humans; D007910:Lenses, Intraocular / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction; D011183:Postoperative Complications; D011425:Propionibacterium acnes / Q000235:genetics / Q000302:isolation & purification*; D019591:Pseudophakia / Q000382:microbiology* / Q000601:surgery
D004269:DNA, Bacterial