Film from peroxidation of an amino phospholipid and its biocompatibility.
A new kind of phospholipid product with fluorescence was derived from autoxidation of an aminophospholipid, L-alpha-phosphatidylethanolamine dilinoleoyl in solid film state. Atomic force microscopy showed that the film product was composed of round and elliptical vesicles with diameters of about 20-45 nm. The product was difficult to dissolve in water and most organic solvents. Fourier transform infrared spectroscopy, solid-state (13)C NMR, and solid-state (31)P NMR indicated that the unsaturated double carbon bond decreased with the reaction, and polymerization within/between phospholipid molecules occurred. Endothelial cells from human vein seeded onto the film showed the highest proliferating activity compared with seeding onto Corning culture plate, glass plate, or phospholipid film with phosphorylcholine head group, as evaluated by 3-(4,5-timethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (p < 0.001). The hemocompatibility of the film was also assessed by platelet adhesion and protein adsorption. The results suggest that the product has potential applications as a new biomaterial coating.
Wang
Hua-Jie
HJ
School of Life Science and Biotechnology, Shanghai Jiaotong University, 800 Dong-Chuan Road, Shanghai 200240, People's Republic of China.
Liu
Xin-Ming
XM
Ji
Li-Wen
LW
Ma
Dong-Lan
DL
Ren
Qiushi
Q
Wang
Jin-Ye
JY
D001672:Biocompatible Materials; D002478:Cells, Cultured; D042783:Endothelial Cells; D006801:Humans; D015227:Lipid Peroxidation; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines
C508476:1,2-dilinolenoylphosphatidylcholine; D001672:Biocompatible Materials; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C038191:1,2-dilinoleoyl-3-phosphatidylethanolamine