Attenuation of abnormalities in the lipid metabolism during experimental myocardial infarction induced by isoproterenol in rats: beneficial effect of ferulic acid and ascorbic acid.
The present study aims at evaluating the effect of the combination of ferulic acid and ascorbic acid on isoproterenol-induced abnormalities in lipid metabolism. The rats were divided into eight groups: Control, isoproterenol, ferulic acid alone, ascorbic acid alone, ferulic acid+ascorbic acid, ferulic acid+isoproterenol, ascorbic acid+isoproterenol and ferulic acid+ascorbic acid+isoproterenol. Ferulic acid (20 mg/kg b.w.t.) and ascorbic acid (80 mg/kg b.w.t.) both alone and in combination was administered orally for 6 days and on the fifth and the sixth day, isoproterenol (150 mg/kg b.w.t.) was injected intraperitoneally to induce myocardial injury to rats. Induction of rats with isoproterenol resulted in a significant increase in the levels of triglycerides, total cholesterol, free fatty acids, free and ester cholesterol in both serum and cardiac tissue. A rise in the levels of phospholipids, lipid peroxides, low density lipoprotein and very low density lipoprotein-cholesterol was also observed in the serum of isoproterenol-intoxicated rats. Further, a decrease in the level of high density lipoprotein in serum and in the phospholipid levels, in the heart of isoproterenol-intoxicated rats was observed, which was paralleled by abnormal activities of lipid metabolizing enzymes: total lipase, cholesterol ester synthase, lipoprotein lipase and lecithin: cholesterol acyl transferase. Pre-cotreatment with the combination of ferulic acid and ascorbic acid significantly attenuated these alterations and restored the levels to near normal when compared to individual treatment groups. Histopathological observations were also in correlation with the biochemical parameters. These findings indicate the synergistic protective effect of ferulic acid and ascorbic acid on isoproterenol-induced abnormalities in lipid metabolism.
Yogeeta
Surinder Kumar
SK
Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600 025, Tamil Nadu, India.
Hanumantra
Rao Balaji Raghavendran
RB
Gnanapragasam
Arunachalam
A
Senthilkumar
Subramanian
S
Subhashini
Rajakannu
R
Devaki
Thiruvengadam
T
D000318:Adrenergic beta-Agonists; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D002316:Cardiotonic Agents / Q000008:administration & dosage / Q000494:pharmacology; D003373:Coumaric Acids / Q000008:administration & dosage / Q000494:pharmacology*; D004338:Drug Combinations; D006321:Heart / Q000187:drug effects; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000494:pharmacology; D007545:Isoproterenol; D008049:Lipase / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008297:Male; D009203:Myocardial Infarction / Q000097:blood / Q000139:chemically induced / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D002787:Sterol Esterase / Q000378:metabolism
D000318:Adrenergic beta-Agonists; D000975:Antioxidants; D002316:Cardiotonic Agents; D003373:Coumaric Acids; D004338:Drug Combinations; D000960:Hypolipidemic Agents; D008055:Lipids; C004999:ferulic acid; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; C031695:acid cholesterol ester synthetase; D002787:Sterol Esterase; D008049:Lipase; D007545:Isoproterenol; D001205:Ascorbic Acid