title: Regulation of PLAP-1 expression in periodontal ligament cells.
abstract: Periodontal-ligament-associated protein-1 (PLAP-1) is preferentially expressed in the periodontal ligament (PDL) and encodes a novel small leucine-rich repeat proteoglycan protein. PLAP-1 expression was induced during the course of cytodifferentiation of PDL cells into mineralized-tissue-forming cells in vitro, suggesting the possible involvement of PLAP-1 in the mineralization process of PDL cells. In this study, we hypothesized that PLAP-1 expression is regulated by mineralization-related cytokines in PDL cells. PLAP-1 expression was clearly down-regulated when the cytodifferentiation of PDL cells was reversibly inhibited by fibroblast growth factor-2 (FGF-2). In contrast, bone morphogenetic protein-2 (BMP-2) enhanced PLAP-1 expression. Up-regulation of PLAP-1 expression by BMP-2 was confirmed at the protein level when PDL cells were immunostained with anti-PLAP-1 polyclonal antibody. These results revealed the cytokine-mediated regulatory mechanisms of PLAP-1 expression and suggested that PLAP-1 expression may be associated with the process of cytodifferentiation of PDL cells.
authors.affiliation: Department of Periodontology, Osaka University Graduate School of Dentistry, Suita, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Ozawa
authors.forename: M
authors.initials: M
authors.lastname: Tomoeda
authors.forename: R
authors.initials: R
authors.lastname: Matoba
authors.forename: K
authors.initials: K
authors.lastname: Matsubara
authors.forename: S
authors.initials: S
authors.lastname: Murakami
mesh_terms: D000595:Amino Acid Sequence; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014074:Tooth Calcification / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: C439889:ASPN protein, human; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins; D016326:Extracellular Matrix Proteins; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2