title: In vitro bioactivity and osteoblast response of porous NiTi synthesized by SHS using nanocrystalline Ni-Ti reaction agent.
abstract: Porous NiTi with an average porosity of 55 vol % and a general pore size of 100-600 microm was synthesized by self-propagating high temperature synthesis (SHS) with the addition of mechanically alloyed nanocrystalline Ni-Ti as the reaction agent. The SHS of porous NiTi using elemental powders was also performed for comparison. To enhance the bioactivity of the metal surface, porous NiTi synthesized by nanocrystalline Ni-Ti was subjected to chemical treatment to form a layer of TiO(2) coating. The porous NiTi with TiO(2) coating was subsequently immersed in a simulated body fluid (SBF) to investigate its apatite forming ability. The effects of the addition of nanocrystalline Ni-Ti as reaction agent and the application of apatite coating on osteoblastic behavior were studied in primary cultures of human osteoblast cells. Results showed that the main phases in porous NiTi synthesized by elemental powders were NiTi, Ti(2)Ni, and unreacted free Ni. By using nanocrystalline Ni-Ti as reaction agent, the secondary intermetallic phase of Ti(2)Ni was significantly reduced and the free Ni was eliminated. TiO(2) coating with anatase phase was formed on the surface of porous NiTi after the chemical treatment. A layer consisting of nanocrystalline carbonate-containing apatite was formed on the surface of TiO(2) coating after soaking in SBF. The preliminary cell culture studies showed that the porous NiTi synthesized with the addition of nanocrystalline Ni-Ti attracted marked attachment and proliferation of the osteoblast cells. This gives the evidence of the potential biomedical applications of the porous NiTi.
authors.affiliation: Singapore Institute of Manufacturing Technology, 71 Nanyang Drive, Singapore 638075, Singapore. mywgu@ntu.edu.sg
authors.forename: Y W
authors.initials: YW
authors.lastname: Gu
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: B Y
authors.initials: BY
authors.lastname: Tay
authors.forename: C S
authors.initials: CS
authors.lastname: Lim
authors.forename: M S
authors.initials: MS
authors.lastname: Yong
authors.forename: K A
authors.initials: KA
authors.lastname: Khor
mesh_terms: D001672:Biocompatible Materials; D003460:Crystallization; D008854:Microscopy, Electron; D049329:Nanostructures; D009532:Nickel; D010006:Osteoblasts / Q000502:physiology*; D016062:Porosity; D013499:Surface Properties; D014025:Titanium; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D009532:Nickel; D014025:Titanium