Effects of resveratrol on mast cell degranulation and tyrosine phosphorylation of the signaling components of the IgE receptor.
The molecular mechanism of how resveratrol inhibits mast cell degranulation was studied by examining its effects on the signaling components of the high affinity IgE receptor (FcepsilonRI) pathway. Resveratrol inhibited mast cell degranulation in a dose-dependent manner and reduced the FcepsilonRI-mediated tyrosine phosphorylation of ERK and PLCgamma1 but not of Syk and PLCgamma2. U-73 122 and PD98059, which are PLC and MEK inhibitors, also had inhibitory effects on mast cell degranulation. These results suggest that FcepsilonRI-mediated tyrosine phosphorylation of PLCgamma1 and ERK could be potential cellular targets of resveratrol for the inhibition of mast cell degranulation.
Koo
NaYeon
N
Department of Pharmacology, College of Pharmacy, Chonnam National University, Kwang-Ju, Korea.
Cho
DongIm
D
Kim
YoungRan
Y
Choi
Hyun Jin
HJ
Kim
Kyeong-Man
KM
D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects*; D045744:Cell Line, Tumor; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D010766:Phosphorylation / Q000187:drug effects; D011505:Protein-Tyrosine Kinases / Q000187:drug effects*; D051381:Rats; D017455:Receptors, IgE / Q000187:drug effects*; D000077185:Resveratrol; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*; D014703:Veratrum / Q000737:chemistry
D017455:Receptors, IgE; D013267:Stilbenes; D011505:Protein-Tyrosine Kinases; D000077185:Resveratrol