Immunohistochemical findings after LASIK confirm in vitro LASIK model.
OBJECTIVE
To compare immunohistochemical findings in human donor corneas after successful laser in situ keratomileusis (LASIK) without clinical complications with a recently established human LASIK in vitro model.


METHODS
Donor corneas with prior LASIK treatment were investigated. Cryostat sections were stained immunohistochemically for collagen types I, III, and VI and laminin and fibronectin.


RESULTS
With light microscopy, the interface of the LASIK flap could hardly be detected. In all samples, fibronectin was consistently detected along the entire extent of the surgical wound. In contrast, collagen type III and laminin only stained the superficial portion of the LASIK incision site. Staining for collagen types I and VI showed no changes after LASIK.


CONCLUSIONS
Histologic findings in donor corneas with prior LASIK treatment confirm histologic observations in a recently introduced human organ culture LASIK model. This strengthens the reliability of the latter LASIK model for further studies concerning wound healing after LASIK surgery.
Priglinger
Siegfried G
SG
Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
May
Christian-Albrecht
CA
Alge
Claudia S
CS
Wolf
Armin
A
Neubauer
Aljoscha S
AS
Haritoglou
Christos
C
Kampik
Anselm
A
Welge-Lussen
Ulrich
U
D000328:Adult; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D024142:Collagen Type VI / Q000378:metabolism; D003315:Cornea / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D020731:Keratomileusis, Laser In Situ; D007797:Laminin / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D009216:Myopia / Q000378:metabolism* / Q000601:surgery; D009924:Organ Culture Techniques; D014019:Tissue Donors; D014945:Wound Healing / Q000502:physiology*
D024042:Collagen Type I; D024061:Collagen Type III; D024142:Collagen Type VI; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin