Dereplication of pentacyclic triterpenoids in plants by GC-EI/MS.
Three common plant-derived pentacyclic triterpenoids, oleanolic acid (1), betulinic acid (2) and ursolic acid (3), have been found to exhibit moderate anti-tubercular activity in a microplate alamar blue assay. In order to facilitate the discovery of novel anti-tubercular leads with diverse chemical structures, a new and rapid GC-EI/MS method was developed simultaneously and unambiguously to dereplicate 1-3 as their methyl esters with limits of detection of 25.6, 26.9 and 26.8 ng, respectively.
Gu
Jian-Qiao
JQ
Department of Pharmacology and Toxicology, Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Arizona, Tucson, AZ 85721, USA.
Wang
Yuehong
Y
Franzblau
Scott G
SG
Montenegro
Gloria
G
Timmermann
Barbara N
BN
D000995:Antitubercular Agents / Q000737:chemistry*; D002849:Chromatography, Gas; D027523:Lavandula / Q000737:chemistry*; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000737:chemistry*; D053978:Pentacyclic Triterpenes; D021241:Spectrometry, Mass, Electrospray Ionization; D020903:Tanacetum parthenium / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry*; D014632:Valerian / Q000737:chemistry*; D000094062:Betulinic Acid; D000097245:Ursolic Acid
D000995:Antitubercular Agents; D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D000094062:Betulinic Acid; D000097245:Ursolic Acid