title: Amphotericin B tissue distribution in autopsy material after treatment with liposomal amphotericin B and amphotericin B colloidal dispersion.
abstract: OBJECTIVE
  Tissue concentrations of amphotericin B were determined in autopsy material of patients who had been treated with liposomal amphotericin B or amphotericin B colloidal dispersion (colloidal amphotericin B) for suspected or proven invasive fungal infection.
  
  
  METHODS
  Amphotericin B tissue levels were measured in liver, spleen, lung, kidney, and myocardial and brain tissue of 20 patients who had been treated with lipid-formulated amphotericin B, before they died from multi-organ failure. Seven patients had been treated with liposomal amphotericin B (AmBisome) and thirteen with colloidal amphotericin B (Amphocil). Tissue samples were obtained during routine autopsy, homogenized and extracted with methanol. Amphotericin B concentrations were measured using HPLC after purification by solid phase extraction.
  
  
  RESULTS
  The highest amphotericin B levels were found in liver and spleen, followed by kidney, lung, myocardium and brain. In the lung higher amphotericin B concentrations were found after treatment with amphotericin B colloidal dispersion than after therapy with liposomal amphotericin B.
  
  
  CONCLUSIONS
  The choice of lipid formulation may influence amphotericin B penetration into the lung.
authors.affiliation: Clinical Pharmacokinetics Unit, Laboratory of Inflammation Research, Division of General Internal Medicine, Department of Internal Medicine, Innsbruck Medical School, Anichstrasse 35, A-6020 Innsbruck, Austria.
authors.forename: Helene
authors.initials: H
authors.lastname: Vogelsinger
authors.forename: Stefan
authors.initials: S
authors.lastname: Weiler
authors.forename: Angela
authors.initials: A
authors.lastname: Djanani
authors.forename: Jordan
authors.initials: J
authors.lastname: Kountchev
authors.forename: Rosa
authors.initials: R
authors.lastname: Bellmann-Weiler
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Wiedermann
authors.forename: Romuald
authors.initials: R
authors.lastname: Bellmann
mesh_terms: D000328:Adult; D000368:Aged; D000666:Amphotericin B / Q000032:analysis* / Q000493:pharmacokinetics; D001344:Autopsy; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure; D009181:Mycoses / Q000150:complications / Q000188:drug therapy; D009206:Myocardium / Q000737:chemistry; D013154:Spleen / Q000737:chemistry; D014018:Tissue Distribution; D014020:Tissue Extracts / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D014020:Tissue Extracts; C068538:liposomal amphotericin B; D000666:Amphotericin B