Construction and characterization of Bacillus subtilis deletion mutants lacking the prophage 2-trnS region.
During development of a novel method for constructing a series of deletions in Bacillus subtilis using an isogenic set of gene-disrupted mutants created by integration of pMutin, deletion of the trnS operon, consisting of seven tRNA genes, was found to affect cell growth, development of competence and spore formation. A suppressor (sts1) of the DeltatrnS mutant was isolated, sequenced and found to have undergone a single base change, CAG to GAG, in the first anticodon of tRNA(Leu), in the trnB operon.
Akanuma
Genki
G
Laboratory of Molecular Genetics and Frontier Project Life's Adaptation Strategies to Environmental Changes, Department of Life Science, College of Science, Rikkyo University, Tokyo, Japan.
Habu
Chiemi
C
Natori
Yousuke
Y
Murayama
Rikinori
R
Nanamiya
Hideaki
H
Kawamura
Fujio
F
D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D001483:Base Sequence; D005798:Genes, Bacterial; D009876:Operon; D039002:Prophages / Q000235:genetics; D012356:RNA, Transfer, Leu / Q000235:genetics; D011995:Recombination, Genetic; D017384:Sequence Deletion; D013171:Spores, Bacterial; D013489:Suppression, Genetic
D012356:RNA, Transfer, Leu