Molecular cloning and preliminary function study of a novel human gene, TSARG7, related to spermatogenesis.
A novel human gene TSARG7 (GenBank accession No. AY513610) was identified from a human testis cDNA library by using the mTSARG7 gene (GenBank accession No. AY489184) as an electronic probe. The gene whose full cDNA length is 2,463 bp containing 12 exons and 11 introns is located in the human chromosome 8p11.21. The predicted protein encoded by this gene contains 456 amino acids with a theoretical molecular weight of 56,295 dalton and isoelectric point of 9.13. It is a new member of the acyltransferase family since its sequence possesses the highly conserved PlsC domain existing in all acyltransferase-like proteins. Two groups, the TSARG7 and mTSARG7, the TSARG7 and Au041707, share 97% identity in the 456 amino acids. Expression of the TSARG7 gene is restricted to the testis. Subcellular localization studies show that the EGFP-tagged TSARG7 protein was localized in the cytoplasm of GC-1 cells. The TSARG7 mRNA expression was initiated in the testis of a 13-year-old boy, and its level increased steadily along with spermatogenesis and sexual maturation of the human. The results of heat stress experiment demonstrate that TSARG7 expression has a relation with temperature. In conclusion, our study suggests that we have cloned a novel human gene and this gene may play an important role in human spermatogenesis and sexual maturation.
Tan
Xiao-Jun
XJ
Human Reproductive and Stem Cell Engineering Institute, Central South University, Changsha, China.
Huang
Zhi-Ping
ZP
Li
Lu-Yun
LY
Nie
Dong-Song
DS
Zhong
Chang-Gao
CG
Fu
Jun-Jiang
JJ
Lu
Guang-Xiu
GX
D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics*; D005333:Fetus; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000235:genetics; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000378:metabolism; D012741:Sexual Maturation / Q000235:genetics; D013091:Spermatogenesis / Q000235:genetics*; D013696:Temperature; D013737:Testis / Q000254:growth & development / Q000378:metabolism*
D018076:DNA, Complementary; D015513:Oncogene Proteins; D012333:RNA, Messenger; C504374:TSARG7 protein, mouse; C529123:GPAT4 protein, human; D005992:Glycerol-3-Phosphate O-Acyltransferase