Suppressed disassembly of autolyzing p94/CAPN3 by N2A connectin/titin in a genetic reporter system.
p94/calpain 3 is a skeletal muscle-specific member of the Ca(2+)-regulated cytosolic cysteine protease family, the calpains. Defective p94 protease activity originating from gene mutations causes a muscular dystrophy called calpainopathy, indicating the indispensability of p94 for muscle survival. Because of the existence of the p94-specific regions IS1 and IS2, p94 undergoes very rapid and exhaustive autolysis. To elucidate the physiological relevance of this unique activity, the autolytic profiles of p94 and the effect of the p94 binding protein, connectin/titin, on this process were investigated. In vitro analysis of p94 autolysis showed that autolysis in IS1 proceeds without immediate disassembly into fragments and that the newly identified cryptic autolytic site in IS2 is critical for disassembling autolyzed fragments. As a genetic system to assay p94 autolysis semiquantitatively, p94 was expressed in yeast as a hybrid protein between the DNA binding and activation domains of the yeast transcriptional activator Gal4. Transcriptional activation by the Gal4-p94:WT hybrid protein is precluded by p94 autolysis. Complete or partial loss of autolytic activity by C129S active site mutation, limb girdle muscular dystrophy type 2A pathogenic missense mutations, or PCR-based random mutagenesis could be detected by semiquantitative restoration of Gal4-dependent beta-galactosidase gene expression. Using this system, the N2A connectin fragment that binds to p94 was shown to suppress p94 autolytic disassembly. The proximity of the IS2 autolytic and connectin-binding sites in p94 suggested that N2A connectin suppresses IS2 autolysis. These data indicate the importance of p94-connectin interaction in the control of p94 functions by regulating autolytic decay of p94.
Ono
Yasuko
Y
Department of Enzymatic Regulation for Cell Functions, Tokyo Metropolitan Institute of Medical Science (Rinshoken), Tokyo 113-8613, Japan.
Torii
Fukuyo
F
Ojima
Koichi
K
Doi
Naoko
N
Yoshioka
Katsuhide
K
Kawabata
Yukiko
Y
Labeit
Dietmar
D
Labeit
Siegfried
S
Suzuki
Koichi
K
Abe
Keiko
K
Maeda
Tatsuya
T
Sorimachi
Hiroyuki
H
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D019556:COS Cells; D002154:Calpain / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D002522:Chlorocebus aethiops; D064211:Connectin; D004789:Enzyme Activation; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D011485:Protein Binding; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae
D064211:Connectin; D009124:Muscle Proteins; D011994:Recombinant Proteins; C577285:TTN protein, human; D011494:Protein Kinases; C105884:CAPN3 protein, human; D002154:Calpain; C491373:Capn3 protein, mouse