title: Increased number of anaerobic bacteria in the infected root canal in type 2 diabetic rats.
abstract: OBJECTIVE
  The purpose of this study was to investigate the relationship between type 2 diabetes mellitus and anaerobic bacteria detected in infected root canals.
  
  
  METHODS
  Normal Wistar rats (control) received a standard laboratory diet with water (group A), and GK rats (type 2 diabetes mellitus rats) a normal laboratory diet with water (group B) or a 30% sucrose solution (group C). Chemotaxis assay was conducted on polymorphonuclear leukocytes from the 3 groups, and the numbers of anaerobic bacteria in infected root canals were determined.
  
  
  RESULTS
  In the chemotaxis assay on the polymorphonuclear leukocytes, the chemotactic response of cells in group C was lower than that for groups A and B (P < .01). As to bacteria detected in the root canal, obligate anaerobic bacteria which stained gram negative, were significantly more numerous in group C (P < .01) than in groups A and B.
  
  
  CONCLUSIONS
  The metabolic condition produced by type 2 diabetes mellitus in rats might lower the general host resistance against bacterial infection.
authors.affiliation: Department of Endodontics and Research Institute of Advanced Oral Science, School Of Dentistry, Aichi-Gakuin University, Nagoya, Japan. akihiro@dpc.aichi-gakuin.ac.jp
authors.forename: Akihiro
authors.initials: A
authors.lastname: Iwama
authors.forename: Taisuke
authors.initials: T
authors.lastname: Morimoto
authors.forename: Masahito
authors.initials: M
authors.lastname: Tsuji
authors.forename: Koki
authors.initials: K
authors.lastname: Nakamura
authors.forename: Naoya
authors.initials: N
authors.lastname: Higuchi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Imaizumi
authors.forename: Naoki
authors.initials: N
authors.lastname: Shibata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D001424:Bacterial Infections / Q000150:complications* / Q000276:immunology / Q000382:microbiology; D002634:Chemotaxis, Leukocyte; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003790:Dental Pulp Necrosis / Q000150:complications / Q000276:immunology / Q000382:microbiology*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000276:immunology; D004195:Disease Models, Animal; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D009504:Neutrophils / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar