title: Mycothiol-dependent mycobacterial response to oxidative stress.
abstract: The effect of exogenous oxidative stress on mycothiol (MSH) levels and redox balance was investigated in mycobacteria. Both the thiol-specific oxidant diamide and hydrogen peroxide induced up to 75% depletion of MSH to form the disulfide form, mycothione (MSSM), in Mycobacterium bovis BCG. In comparison, Mycobacterium smegmatis, a saprophytic mycobacterium, displays a greater tolerance towards these oxidants, reflected by the lack of fluxes in MSH levels and redox ratios upon oxidative stress treatments. The basal ratio of MSH to MSSM was established to be 50:1 in M. bovis BCG and 200:1 in M. smegmatis.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, The University of British Columbia, D-452 Heather Pavilion East, 2733 Heather Street, Vancouver, BC, Canada V5Z 3J5.
authors.forename: Korine S E
authors.initials: KS
authors.lastname: Ung
authors.forename: Yossef
authors.initials: Y
authors.lastname: Av-Gay
mesh_terms: D003545:Cysteine; D004187:Disaccharides / Q000378:metabolism*; D006020:Glycopeptides; D007294:Inositol; D009163:Mycobacterium bovis / Q000187:drug effects / Q000378:metabolism*; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D011720:Pyrazoles / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D014867:Water / Q000494:pharmacology
chemical_list: D004187:Disaccharides; D006020:Glycopeptides; D011720:Pyrazoles; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; C089265:mycothiol; D014867:Water; D007294:Inositol; D003545:Cysteine