[Effects of estradiol intervention on peripheral blood CD90+ cells and cardiac function in de-ovary rats before and after myocardial infarction].
OBJECTIVE
To investigate the effects of estradiol intervention on peripheral blood CD90+ cells and cardiac function in de-ovary rats before and after myocardial infarction.


METHODS
Thirty SD female rats were divided into the control group, acute myocardial infarction group, de-ovary myocardial infarction group, de-ovary replacement group with estradiol, and de-ovary therapy group with estradiol. CD90+ cell percentage was determined with flow cytometry. The left ventricle end systolic pressure (LVESP), left ventricle end diastolic pressure (LVEDP), +dp/dt(max) and -dp/dt(max) were also measured with the POWERLAB4.12 system.


RESULTS
The percentage of peripheral blood CD90+ cells in the de-ovary myocardial infarction group was remarkably lower than that in the acute myocardial infarction group and the de-ovary replacement group with estradiol (P<0.01). In the de-ovary replacement group with estradiol, the percentage of peripheral blood CD90+ cells was remarkably elevated following the first day of operation (P<0.05), reaching the peak value at day 3. In the de-ovary therapy group with estradiol, it started to elevate at day 7 after the operation, much lower than that in the de-ovary replacement group with estradiol. LVESP in each operation group decreased, especially in the acute myocardial infarction group, whereas LVEDP increased in the de-ovary group and de-ovary therapy group with estradiol (P<0.01).


CONCLUSIONS
Replacement with estradiol before myocardial infarction exerts more beneficial effects than estradiol replacement after myocardial infarction in de-ovary rats.
Zheng
Xiao-pu
XP
Department of Cardiology, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Cardiovascular Ion Channelopathy Laboratory, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. zhengxp001@163.com
Ma
Ai-qun
AQ
Han
Ke
K
Liu
Yu
Y
Zhang
Wei
W
Wang
Rui
R
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002320:Cardiovascular Physiological Phenomena; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006321:Heart / Q000187:drug effects; D006331:Heart Diseases / Q000473:pathology*; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D009203:Myocardial Infarction / Q000097:blood / Q000473:pathology*; D009206:Myocardium / Q000473:pathology; D010052:Ovariectomy / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000601:surgery*; D018800:Thy-1 Antigens / Q000378:metabolism*
D018800:Thy-1 Antigens; D004958:Estradiol