title: Influence of xanthohumol on the binding behavior of GABAA receptors and their lateral mobility at hippocampal neurons.
abstract: The influence of the xanthohumol from Humulus lupulus L. on the binding of muscimol-Alexa Fluor 532 (Mu-Alexa), a fluorescently labeled GABAA receptor agonist, was studied by fluorescence correlation spectroscopy. An incubation of hippocampal neurons with 75 nM of xanthohumol increased the specific Mu-Alexa binding by approximately 17%, which was selectively found in GABAA receptor Mu-Alexa complexes with hindered lateral mobility [D(bound2) = (0.11 +/- 0.03) microm2/s] as described with midazolam.
authors.affiliation: Department of Pharmaceutical Biology, University of Marburg, Marburg, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Meissner
authors.forename: Hanns
authors.initials: H
authors.lastname: Häberlein
mesh_terms: D000818:Animals; D005419:Flavonoids; D006624:Hippocampus / Q000166:cytology*; D027582:Humulus / Q000737:chemistry; D066298:In Vitro Techniques; D009118:Muscimol; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011427:Propiophenones / Q000494:pharmacology*; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D005419:Flavonoids; D011427:Propiophenones; D011963:Receptors, GABA-A; D009118:Muscimol; C104536:xanthohumol