Sequence-selective osmium oxidation of DNA: efficient distinction between 5-methylcytosine and cytosine.
5-Methylcytosine was distinguished from cytosine using the large difference of their osmium oxidation rates, and this reaction was applied to detection of the cytosine methylation status at a specific site of a long sequence using the formation of a bulge structure by hybridization with a guide DNA.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan. okamoto@sbchem.kyoto-u.ac.jp
Tainaka
Kazuki
K
Kamei
Taku
T
D044503:5-Methylcytosine / Q000032:analysis*; D001483:Base Sequence; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008722:Methods; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction
D009992:Osmium; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA