Measurement of patients' bivalirudin plasma levels by a thrombelastograph ecarin clotting time assay: a comparison to a standard activated clotting time.
Standard activated clotting time (ACT) tests have a poor correlation to bivalirudin levels, leading to uncertainty regarding adequate anticoagulation in percutaneous coronary intervention patients. We tested a Thrombelastograph (TEG) ecarin clotting time (ECT) assay for sensitivity to bivalirudin using blood from 80 patients undergoing interventional cardiology procedures with bivalirudin anticoagulation. This was compared to a standard Hemochron ACT assay using diatomaceous earth. With the TEG assay, the direct thrombin activator, ecarin, was used to initiate coagulation and measured as the reaction time. Plasma samples were evaluated for bivalirudin by a chromogenic assay at an independent hematological laboratory. Linear regression of the standard ACT versus bivalirudin level gave an r = 0.306 whereas the TEG ECT gave a much higher r2 = 0.746 (both P < 0.0001). The TEG ECT should prove more useful than the standard ACT for monitoring bivalirudin anticoagulation across the clinically therapeutic range.
Carroll
Roger C
RC
Department of Anesthesiology, University of Tennessee Graduate School of Medicine, Knoxville, Tennessee 37920, USA.
Chavez
Jack J
JJ
Simmons
Jeffery W
JW
Snider
Carolyn C
CC
Wortham
Dale C
DC
Bresee
Stuart J
SJ
Cohen
Eli
E
D001777:Blood Coagulation / Q000502:physiology*; D001780:Blood Coagulation Tests / Q000379:methods; D010450:Endopeptidases; D006629:Hirudins; D006801:Humans; D016014:Linear Models; D010446:Peptide Fragments; D011994:Recombinant Proteins; D013916:Thrombelastography / Q000379:methods*; D014914:Whole Blood Coagulation Time / Q000379:methods
D006629:Hirudins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D010450:Endopeptidases; C020097:ecarin; C074619:bivalirudin