Delayed cuticular penetration and enhanced metabolism of deltamethrin in pyrethroid-resistant strains of Helicoverpa armigera from China and Pakistan.
The penetration and metabolism of [14C]deltamethrin was studied in susceptible and resistant Chinese and Pakistani strains of Helicoverpa armigera (Hübner), which were resistant to deltamethrin by 330- and 670-fold, respectively. The penetration of deltamethrin into resistant individuals was significantly slower than into susceptible individuals over a 24-h period. The time taken for 50% penetration of the applied deltamethrin was 1 h for the susceptible strain and 6 h for both of the resistant strains. The internal radioactivity was reduced by the larvae of resistant strains much faster than by the susceptible larvae. After 48 h, 40% of the penetrated deltamethrin was still inside the larvae of the susceptible strain, in comparison with 1.5-5% in the Pakistani strain and 8-14% in the Chinese strain. Both of the resistant strains produced methanol-soluble and water-soluble metabolites, but the susceptible strain produced methanol-soluble metabolites only. By 12, 24 and 48 h after dosing, the amount of methanol-soluble metabolites excreted by the resistant strains was almost double that of the susceptible strain. Both of the resistant strains also excreted 5-7% of the penetrated dose as a water-soluble metabolite after 48 h. In comparison with the Chinese strain, the Pakistani strain exhibited slower penetration, lower internal content and faster excretion of deltamethrin, which correlated with the higher resistance of the Pakistani strain. These findings show that the resistant Pakistani and Chinese strains of H. armigera possess mechanisms of reduced cuticular penetration and enhanced metabolism of deltamethrin and perhaps other pyrethroids.
Ahmad
Mushtaq
M
Central Cotton Research Institute, Multan, Pakistan. mush_a@yahoo.com
Denholm
Ian
I
Bromilow
Richard H
RH
D000818:Animals; D002250:Carbon Radioisotopes / Q000032:analysis; D002681:China; D007305:Insecticide Resistance; D007306:Insecticides / Q000493:pharmacokinetics*; D007814:Larva / Q000378:metabolism / Q000502:physiology; D009036:Moths / Q000378:metabolism* / Q000502:physiology; D009570:Nitriles / Q000493:pharmacokinetics*; D010154:Pakistan; D011722:Pyrethrins / Q000493:pharmacokinetics* / Q000494:pharmacology; D012995:Solubility
D002250:Carbon Radioisotopes; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin