Suppression of Mycobacterium tuberculosis induced reactive oxygen species (ROS) and TNF-alpha mRNA expression in human monocytes by allicin.
Chronic inflammation associated with tumor necrosis factor (TNF)-alpha and reactive oxygen species (ROS) is the hallmark of tuberculosis. Mycobacterium tuberculosis (MTB) directly stimulates human monocytes to secrete TNF-alpha. We show the augmented expression of TNF-alpha mRNA in MTB-infected monocytes by cellular activation and ROS was suppressed by allicin in a dose-dependent manner. Also, allicin enhanced the glutathione peroxidase activity, which correlated inversely with the downregulation of ROS and TNF-alpha in MTB-infected monocytes. Hence, allicin may prove to be a valuable natural antioxidant in combating tuberculosis.
Hasan
Nazarul
N
Department of Biochemistry, Faculty of Medicine, J.N. Medical College, A.M.U., Aligarh 202002, U.P., India.
Yusuf
Nabiha
N
Toossi
Zahra
Z
Islam
Najmul
N
D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D004220:Disulfides; D004305:Dose-Response Relationship, Drug; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013441:Sulfinic Acids / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D017931:DNA Primers; D004220:Disulfides; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D013441:Sulfinic Acids; D014409:Tumor Necrosis Factor-alpha; C006452:allicin; D005979:Glutathione Peroxidase