title: Increased serum phosphodiesterase activity in women with pre-eclampsia.
abstract: OBJECTIVE
  To measure the activity of serum phosphodiesterase (PDE) in pre-eclampsia.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  Nephrology Laboratory and Obstetrics Unit at São Lucas Hospital from Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
  
  
  METHODS
  Twenty-nine normal and 28 pregnant women with pre-eclampsia.
  
  
  METHODS
  Serum was collected from women with pre-eclampsia, at the time of diagnosis, and from gestation-matched controls. Circulating PDE activity was assessed by measuring consumption of the substrate thymidine 5'-monophosphate p-nitrophenyl ester and expressed as PDE units/l.
  
  
  METHODS
  Serum PDE Activity.
  
  
  RESULTS
  Mean substrate consumption was higher in pre-eclamptic condition (V(max)= 15.8 +/- 1.4 versus 12.7 +/- 0.9 U/L, P= 3.7 x 10(-14)).
  
  
  CONCLUSIONS
  These data suggest that altered PDE activity may play a role in pre-eclampsia endothelial dysfunction.
authors.affiliation: Programa de Pós-graduação em Medicina e Ciências da Saúde (Nefrologia), Instituto de Pesquisas Biomédicas/Faculdade de Medicina/Hospital São Lucas, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil. bart@pucrs.br
authors.forename: B E
authors.initials: BE
authors.lastname: Pinheiro da Costa
authors.forename: C
authors.initials: C
authors.lastname: Scocco
authors.forename: C E
authors.initials: CE
authors.lastname: Poli de Figueiredo
authors.forename: J A
authors.initials: JA
authors.lastname: Guimarães
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000201:enzymology*; D011247:Pregnancy
chemical_list: D010727:Phosphoric Diester Hydrolases