title: American foulbrood of the honey bee: occurrence and distribution of different genotypes of Paenibacillus larvae in the administrative district of Arnsberg (North Rhine-Westphalia).
abstract: Between March 2003 and October 2004, Paenibacillus larvae, the aetiological agent of American foulbrood disease of the honey bee, was isolated from broodcombs and honey samples of 54 apiaries in the administrative district of Arnsberg (North Rhine-Westphalia, Germany). Genotyping of 176 P. larvae isolates with repetitive element polymerase chain reaction fingerprinting (rep-PCR) using BOX A1R and MBO REP1 primers revealed five different genotypes (AB, Ab, ab, ass, Acapital BE, Cyrillic). In samples of three apiaries, more than one genotype was detected. A combination of two genotypes was isolated from honey samples of the same hive two times (ab/ass and Ab/ab). The five genotypes were not randomly distributed in the district, but revealed a certain geographical clustering. Possible factors with impact on the genotype diversity and the distribution pattern are discussed.
authors.affiliation: Staatliches Veterinäruntersuchungsamt Arnsberg, Zur Taubeneiche 10-12, 59821 Arnsberg, Germany. peters@svua-arnsberg.nrw.de
authors.forename: M
authors.initials: M
authors.lastname: Peters
authors.forename: J
authors.initials: J
authors.lastname: Kilwinski
authors.forename: A
authors.initials: A
authors.lastname: Beringhoff
authors.forename: D
authors.initials: D
authors.lastname: Reckling
authors.forename: E
authors.initials: E
authors.lastname: Genersch
mesh_terms: D000818:Animals; D001406:Bacillaceae / Q000145:classification / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000662:veterinary*; D001516:Bees / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000032:analysis*; D005838:Genotype; D005858:Germany; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial