Characterization of the Legionella anisa population structure by pulsed-field gel electrophoresis.
We analysed 38 French isolates of Legionella anisa by means of pulsed-field gel electrophoresis (PFGE) with single or double digestion. Double digestion was more discriminatory than single digestion, and can thus be useful for epidemiological studies of L. anisa. Several isolates from different parts of France clustered together on the basis of their PFGE patterns (similarity cutoff of 80%), suggesting that the L. anisa population structure is homogenous or that a few clones of L. anisa strains have spread widely in France.
Akermi
Mongi
M
Centre National de référence des Legionella, Laboratoire de Bactériologie INSERM E-0230, Faculté de Médecine, Lyon, France.
Doleans
Anne
A
Forey
Françoise
F
Reyrolle
Monique
M
Meugnier
Helene
H
Freney
Jean
J
Vandenesch
François
F
Etienne
Jerome
J
Jarraud
Sophie
S
D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D005602:France; D007875:Legionella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D015183:Restriction Mapping