title: Ochratoxin A in human blood in Abidjan, Côte d'Ivoire.
abstract: Ochratoxin A (OTA) produced by Aspergillus and Penicillium genera contaminates a diversity of foods including cereals; cereals-derived foods; dry fruits; beans; cocoa; coffee; beer; wine; and foodstuffs of animal origin mainly poultry, eggs, pork and milk, including human breast milk. OTA is nephrotoxic to all animal species studied so far and most likely to humans, who show the longest half-life for elimination of this toxin among all species examined. Among other toxic effects, OTA is teratogenic, immunotoxic, genotoxic, mutagenic and carcinogenic, all of which lead to life-threatening pathologies through several molecular pathways. In Côte d'Ivoire, preliminary surveys conducted by us have proven from 1998 to 2004 the reality of ochratoxin A-contamination of foodstuffs. To assess OTA in human blood, the immunoaffinity columns were used along with HPLC for separation and fluorimetric quantification of blood samples collected in Abidjan from two categories of people: apparently healthy donors (n=63) and nephropathy patients undergoing dialysis (n=39). Among healthy donors, 34.9% show OTA concentrations ranging from 0.01 - 5.81 microg/l with a mean value of 0.83 microg/l, whereas, among nephropathy patients undergoing dialysis 20.5% are OTA positive in a range of 0.167-2.42 microg/l and a mean value of 1.05. Although the sex ratio is 0.82 (46 females for 56 males) ochratoxin A contamination is equally distributed in both sexes. Nephropathy patients undergoing dialysis appear, however, less frequently contaminated than healthy donors (20.5 versus 34.9%) and show higher OTA concentrations (higher mean value, p=0.01). Ochratoxin A concentrations found in human blood reflect concentrations previously detected in cereals and peanuts according to the eating habits and diets of people in Côte d'Ivoire. But, the prevalence of ochratoxin A in blood of nephropathy people undergoing dialysis appears lower than expected from the frequency of OTA contamination in cereals and peanuts. Pearson chi(2)-test indicates that among OTA-positive individuals renal dialysis and age are important modalities for consideration.
authors.affiliation: Department of Toxicology, University of Bordeaux 2, 146, rue Léo-Saignat, 33076 Bordeaux, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Sangare-Tigori
authors.forename: Serge
authors.initials: S
authors.lastname: Moukha
authors.forename: James H
authors.initials: JH
authors.lastname: Kouadio
authors.forename: Djédjé Sébastien
authors.initials: DS
authors.lastname: Dano
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Betbeder
authors.forename: Abdellatif
authors.initials: A
authors.lastname: Achour
authors.forename: Edmond E
authors.initials: EE
authors.lastname: Creppy
mesh_terms: D000328:Adult; D000367:Age Factors; D001782:Blood Donors; D007560:Cote d'Ivoire; D005260:Female; D005506:Food Contamination / Q000032:analysis* / Q000706:statistics & numerical data; D006262:Health; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009793:Ochratoxins / Q000097:blood*; D006435:Renal Dialysis; D012744:Sex Ratio
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A