title: Polymorphism of DRD2 gene and ADHD.
abstract: OBJECTIVE
  Attention deficit hyperactivity disorder (ADHD) is a prevalent childhood disorder. Evidence from the family and twin studies suggest that ADHD is familiar and highly heritable. Association studies are frequently used for the searching of markers responsible for genetic basis of ADHD. We investigated TaqI polymorphism of the dopamine receptor D2 (DRD2) in relationship with ADHD. The association between TaqI A polymorphism of DRD2 gene and ADHD has previously been published.
  
  
  METHODS
  We used the association study to test the relationship between TaqI A polymorphism of DRD2 gene and ADHD on groups of ADHD boys and control boys.
  
  
  METHODS
  For DNA isolation, buccal tissue was used. PCR with restriction analysis of PCR products was used for genotyping.
  
  
  RESULTS
  We found statistically different genotypic and allelic frequencies (p < 0.008, p < 0.002, respectively) of DRD2 polymorphism between two studied groups of boys.
  
  
  RESULTS
  According to our results we suppose that polymorphism TaqI A of DRD2 gene is involved in the pathogenesis of childhood ADHD in male subjects. Allele A1 and genotype A1A1 in male subjects is associated with ADHD.
  
  
  CONCLUSIONS
  Our study confirmed the relationship between TaqI A polymorphism of DRD2 gene and ADHD published previously.
authors.affiliation: Laboratory of neurobiology and molecular psychiatry, Department of Biochemistry, Masaryk University, Faculty of Science, Brno, Czech Republic. omarsery@sci.muni.cz
authors.forename: Omar
authors.initials: O
authors.lastname: Serý
authors.forename: Ivana
authors.initials: I
authors.lastname: Drtílková
authors.forename: Pavel
authors.initials: P
authors.lastname: Theiner
authors.forename: Renáta
authors.initials: R
authors.lastname: Pitelová
authors.forename: Radim
authors.initials: R
authors.lastname: Staif
authors.forename: Vladimír
authors.initials: V
authors.lastname: Znojil
authors.forename: Jan
authors.initials: J
authors.lastname: Lochman
authors.forename: William
authors.initials: W
authors.lastname: Didden
mesh_terms: D000293:Adolescent; D000483:Alleles; D001289:Attention Deficit Disorder with Hyperactivity / Q000453:epidemiology* / Q000235:genetics* / Q000523:psychology; D002648:Child; D018153:Czech Republic / Q000453:epidemiology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005787:Gene Frequency; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D011569:Psychiatric Status Rating Scales; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017448:Receptors, Dopamine D2; D004247:DNA