A micro-scale multi-frequency reactance measurement technique to detect bacterial growth at low bio-particle concentrations.
The technique described enables the user to detect the presence and proliferation of bacteria through an increase in the bulk capacitance (C) of the suspension, which is proportional to the bacteria count, at practical frequencies less than 1 MHz. The geometry of the micro-capillary design employed increases the bulk resistance (R) of the medium, thus increasing its RC time. This makes the measured reactance sensitive to changes in the bulk capacitance, which is usually masked by the much larger surface capacitance. The sensitivity is further enhanced by the existence of a minimum in the value of the reactance at a frequency proportional to the inverse medium RC time. The value of this reactance minimum and the frequency at which the minimum is recorded are dependent on the bacteria count and permit the detection of an initial concentration of approximately 100 CFU ml(-1) of E. coli within 3 hours of incubation, in comparison with the previous reported values of about 8 hours, with an initial load of 1000 CFU ml(-1).
Sengupta
Shramik
S
Center for Microfluidics and Medical Diagnostics, Department of Chemical and Biomolecular Engineering, University of Notre Dame, 182 Fitzpatrick Hall, Notre Dame, IN 46556, USA.
Battigelli
David A
DA
Chang
Hsueh-Chia
HC
D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods*; D015169:Colony Count, Microbial; D025802:Electric Capacitance; D004566:Electrodes; D004926:Escherichia coli / Q000166:cytology* / Q000254:growth & development / Q000302:isolation & purification*; D012680:Sensitivity and Specificity