Optimization of wastewater feeding for single-cell protein production in an anaerobic wastewater treatment process utilizing purple non-sulfur bacteria in mixed culture condition.
Impacts of operation timing of feeding and withdrawal on anaerobic wastewater treatment utilizing purple non-sulfur bacteria have been investigated in mixed culture condition with acidogenic bacteria. Simulated wastewater containing glucose was treated in a laboratory-scale chemostat reactor, changing the timing of wastewater feeding and withdrawal. Rhodopseudomonas palustris, which does not utilize glucose as a substrate, was inoculated in the reactor. Rps. palustris was detected by a fluorescent in situ hybridization (FISH) technique using the specific Rpal686 probe. As a result, population ratios of Rps. palustris were over 20% through the operation. Rps. palustris could grow by utilizing metabolites of acidogenic bacteria that coexisted in the reactor. A morning feed was effective for a good growth of purple non-sulfur bacteria. A protein content of cultured bacteria was the highest when wastewater was fed in the morning. Dissolved organic carbon (DOC) removal was 94% independent of the timing control. Consequently, feeding in the morning is the optimum feed-timing control from the aspects of growth of purple non-sulfur bacteria and single-cell protein production.
Honda
Ryo
R
Integrated Research System for Sustainability Science (IR3S), The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8654, Japan. honda@ir3s.u-tokyo.ac.jp
Fukushi
Kensuke
K
Yamamoto
Kazuo
K
D019149:Bioreactors / Q000382:microbiology; D004044:Dietary Proteins / Q000138:chemical synthesis; D008954:Models, Biological; D014176:Protein Biosynthesis; D012241:Rhodopseudomonas / Q000254:growth & development* / Q000378:metabolism*; D012244:Rhodospirillaceae / Q000254:growth & development*; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods
D004044:Dietary Proteins; D012722:Sewage; C012551:single cell proteins