The sensitive determination of nucleic acids using a fluorescence-quenching method.
Experiments indicated that nucleic acids can quench the fluorescence of the Eu3+ -2-thenoyltrifluoroacetone (TTA)-1,10-phenanthroline (Phen) system. Based on this, a sensitive method for the determination of nucleic acids was proposed. The experiments indicated that under the optimum conditions, the quenched fluorescence intensity was in proportion to the concentration of nucleic acids in the range 1.0 x 10(-11)-1.0 x 10(-6) g/mL for yeast RNA (yRNA), 5.0 x 10(-11)-5.0 x 10(-7) g/mL for fish sperm (fsDNA) and 1.0 x 10(-10)-1.5 x 10(-6) g/mL for calf thymus DNA (ctDNA). Their detection limits were 3.0 x 10(-12), 4.0 x 10(-12) and 5.0 x 10(-11) g/mL, respectively. Therefore, the proposed method is one of the most sensitive methods available. The interaction between nucleic acids and Eu3+ -TTA-Phen is also discussed.
Jia
Zhen
Z
Key Laboratory of Colloid and Interface Chemistry of the Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
Yang
Jinghe
J
Wu
Xia
X
Sun
Changxia
C
Wang
Fei
F
Liu
Shufang
S
Wang
Feng
F
D005063:Europium / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013804:Thenoyltrifluoroacetone / Q000737:chemistry
D009696:Nucleic Acids; D009942:Organometallic Compounds; D010618:Phenanthrolines; D012996:Solutions; D013804:Thenoyltrifluoroacetone; D005063:Europium; C025205:1,10-phenanthroline