title: Complete nucleotide sequence of a P2 family lysogenic bacteriophage, varphiMhaA1-PHL101, from Mannheimia haemolytica serotype A1.
abstract: The 34,525 nucleotide sequence of a double-stranded DNA bacteriophage (phiMhaA1-PHL101) from Mannheimia haemolytica serotype A1 has been determined. The phage encodes 50 open reading frames. Twenty-three of the proteins are similar to proteins of the P2 family of phages. Other protein sequences are most similar to possible prophage sequences from the draft genome of Histophilus somni 2336. Fourteen open reading frames encode proteins with no known homolog. The P2 orthologues are collinear in phiMhaA1-PHL101, with the exception of the phage tail protein gene T, which maps in a unique location between the S and V genes. The phage ORFs can be arranged into 17 possible transcriptional units and many of the genes are predicted to be translationally coupled. Southern blot analysis revealed phiMhaA1-PHL101 sequences in other A1 isolates as well as in serotype A5, A6, A9, and A12 strains of M. haemolytica, but not in the related organisms, Mannheimia glucosida or Pasteurella trehalosi.
authors.affiliation: Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA. sarahh@bcm.tmc.edu
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Highlander
authors.forename: Sarah
authors.initials: S
authors.lastname: Weissenberger
authors.forename: Laura E
authors.initials: LE
authors.lastname: Alvarez
authors.forename: George M
authors.initials: GM
authors.lastname: Weinstock
authors.forename: Peter B
authors.initials: PB
authors.lastname: Berget
mesh_terms: D001435:Bacteriophages / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001482:Base Composition; D001483:Base Sequence; D004261:DNA Replication; D004267:DNA Viruses / Q000235:genetics; D004279:DNA, Viral / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D016978:Mannheimia haemolytica / Q000821:virology*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins