Association of phthalate esters with endometriosis in Indian women.
OBJECTIVE
To evaluate the possible association between phthalate esters (PEs) and the occurrence of endometriosis.


METHODS
Case-control study.


METHODS
Department of Reproductive Medicine, Bhagawan Mahavir Medical Research Centre, Maternal Health and Reproductive Institute and Department of Analytical R&D, Hetero Research Foundation, Hyderabad, Andhra Pradesh, India.


METHODS
Blood samples were collected from 49 infertile women with endometriosis (study group); 38 age-matched women without endometriosis (control group I) but with infertility related to tubal defects, fibroids, polycystic ovaries, idiopathic infertility and pelvic inflammatory diseases diagnosed by laparoscopy and a further group of 21 age-matched women (control group II) with proven fertility and no evidence of endometriosis and other gynaecological disorders during laparoscopic sterilisation.


METHODS
Concentrations of PEs were measured using gas chromatography.


METHODS
Evaluation of PEs concentrations in women with endometriosis compared with women free from the disease.


RESULTS
Women with endometriosis showed significantly higher concentrations of di-n-butyl phthalate (DnBP), butyl benzyl phthalate (BBP), di-n-octyl phthalate (DnOP) and diethyl hexyl phthalate (DEHP) (mean 0.44 [SD 0.41]; 0.66 [SD 0.61]; 3.32 [SD 2.17]; 2.44 [SD 2.17] micrograms/ml) compared with control group I (mean 0.08 [SD 0.14]; 0.12 [SD 0.20]; 0; 0.50 [SD 0.80] micrograms/ml) and control group II (mean 0.15 [SD 0.21]; 0.11 [SD 0.22]; 0; 0.45 [SD 0.68] micrograms/ml). The correlation between the concentrations of PEs and different severity of endometriosis was strong and statistically significant at P < 0.05 for all four compounds (DnBP: r=+0.73, P < 0.0001; BBP: r=+0.78, P < 0.0001; DnOP: r=+0.57, P < 0.0001 and DEHP: r=+0.44, P < 0.0014).


CONCLUSIONS
This study suggests that PEs may have an aetiological association with endometriosis.
Reddy
B S
BS
Department of Reproductive Medicine, Bhagwan Mahavir Medical Research Centre, A.C. Guards, Hyderabad-500 004, Andhra Pradesh, India.
Rozati
R
R
Reddy
B V R
BV
Raman
N V V S S
NV
D000328:Adult; D000367:Age Factors; D015992:Body Mass Index; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D004715:Endometriosis / Q000097:blood / Q000139:chemically induced*; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D006801:Humans; D007194:India; D007247:Infertility, Female / Q000097:blood / Q000139:chemically induced; D008572:Menarche; D010795:Phthalic Acids / Q000097:blood / Q000633:toxicity*; D011446:Prospective Studies
D010795:Phthalic Acids