[Study on expression of brain aging-relative genes HSP86 and HSP84 and effects of acupuncture in the SAMP10 mouse].
OBJECTIVE
To probe the mechanism of acupuncture for anti-aging.


METHODS
In the senescence accelerated mouse the SAMP10 and the SAMR1, by using RT-PCR and DIG-labeled Northern blot technique, the expression differences of HSP84 and HSP86 genes in whole brain, cortex and hippocampus in the 4 groups,8-month SAMR1 control group, 8-month SAMP10 control group, 8-month SAMP10 acupuncture group and 8-month SAMP10 non-point acupuncture group were investigated.


RESULTS
In the SAMP10 control group, the expression of HSP84 and HSP86 were down-regulated in the whole brain, the cortex and the hippocampus, and they were up-regulated after acupuncture, tending to the normal group.


CONCLUSIONS
Brain aging of the SAMP10 mouse is related with abnormal expression of HSP84 and HSP86 genes, and acupuncture can strengthen the protection of cells, inhibit apoptosis and anti-oxidative stress through regulating expression of HSP84 and HSP86, hence anti-aging.
Fu
Yu
Y
First Affiliated Hospital of Tianjin College of TCM, Tianjin 300193, China.
Yu
Jian-chun
JC
Ding
Xiao-rong
XR
Han
Jing-xian
JX
D015670:Acupuncture Therapy; D000375:Aging / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis
C488983:HSP84 protein, mouse; D018841:HSP90 Heat-Shock Proteins; C107835:Hspca protein, mouse; D012333:RNA, Messenger