The region in a subunit of the Aspergillus CCAAT-binding protein similar to the HAP4p-recruiting domain of Saccharomyces cerevisiae Hap5p is not essential for transcriptional enhancement.
The CCAAT-binding complex in Aspergillus species, known as the Hap complex, consists of at least three subunits, HapB, HapC, and HapE. Each Hap subunit contains an evolutionarily conserved core domain. In this study, a series of the truncated gene, which encodes the HapE subunit of Aspergillus oryzae, was constructed to survey the regions essential for the transcriptional enhancement of fungal genes. It was revealed that the non-conserved regions and the conserved region similar to the Hap4p recruiting domain of Saccharomyces cerevisiae were not necessary for Hap complex-mediated transcriptional enhancement.
Tanoue
Shinjiro
S
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
Kamei
Ken-ichi
K
Goda
Hideya
H
Tanaka
Akimitsu
A
Kobayashi
Tetsuo
T
Tsukagoshi
Norihiro
N
Kato
Masashi
M
D000595:Amino Acid Sequence; D000681:Amylases / Q000378:metabolism; D001233:Aspergillus nidulans / Q000737:chemistry / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D023081:CCAAT-Binding Factor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002474:Cell-Free System; D002480:Cellulase / Q000378:metabolism; D017124:Conserved Sequence; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism*; D016415:Sequence Alignment; D015534:Trans-Activators / Q000737:chemistry* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics
D023081:CCAAT-Binding Factor; C061437:HAP4 protein, S cerevisiae; C508483:Hap5 protein, S cerevisiae; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000681:Amylases; D002480:Cellulase