title: Growth arrest and decrease of alpha-SMA and type I collagen expression by palmitic acid in the rat hepatic stellate cell line PAV-1.
abstract: Liver fibrosis is characterized by an activation of hepatic stellate cells (HSC). During primary culture HSC evolve from a quiescent into an activated phenotype which is characterized by alpha-smooth muscle actin (alpha-SMA) up-regulation, increase in cell growth, and extracellular matrix secretion. HSC culture with trans-resveratrol can lead to deactivation of myofibroblast-like HSC. We used an HSC line, PAV-1, to check the role of retinol and palmitic acid in the deactivation process of HSC. Using mass and metabolic-based methods, Western blot and immunocytochemistry assays, we demonstrated that treatment with palmitic acid (75 muM) alone or in combination with retinol (2 muM) significantly decreased cell proliferation and alpha-SMA expression. We also established that the association of both compounds strongly decreased collagen type I expression. Our results suggest the potential use of palmitic acid alone or in combination with retinol to induce HSC deactivation.
authors.affiliation: Unité des Maladies Métaboliques et Micro-nutriments, Equipe Vitamines, INRA de Clermont-Fd-Theix, 63122 Saint-Genès, Champanelle, France. aabergel@chu-clermontferrand.fr
authors.forename: Armand
authors.initials: A
authors.lastname: Abergel
authors.forename: Vincent
authors.initials: V
authors.lastname: Sapin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dif
authors.forename: Christophe
authors.initials: C
authors.lastname: Chassard
authors.forename: Claude
authors.initials: C
authors.lastname: Darcha
authors.forename: Julie
authors.initials: J
authors.lastname: Marcand-Sauvant
authors.forename: Brigitte
authors.initials: B
authors.lastname: Gaillard-Martinie
authors.forename: Edmond
authors.initials: E
authors.lastname: Rock
authors.forename: Pierre
authors.initials: P
authors.lastname: Dechelotte
authors.forename: Patrick
authors.initials: P
authors.lastname: Sauvant
mesh_terms: D000199:Actins / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008103:Liver Cirrhosis; D019308:Palmitic Acid / Q000494:pharmacology*; D051381:Rats; D014801:Vitamin A / Q000494:pharmacology*
chemical_list: D000199:Actins; D024042:Collagen Type I; D019308:Palmitic Acid; D014801:Vitamin A; C498862:Acta2 protein, rat