title: Massively parallel signature sequencing profiling of fetal human neural precursor cells.
abstract: We have examined gene expression in multipotent neural precursor cells (NPCs) derived from human fetal (f) brain tissue and compared its expression profiles with embryonic stem (ESC) cells, embryoid body cell (EBC), and astrocyte precursors using the technique of massively parallel signature sequencing (MPSS). Gene expression profiles show that fNPCs express core neural stem cells markers and share expression profiles with astrocyte precursor cells (APCs) rather than ESC or EBC. Gene expression analysis shows that fNPCs differ from other adult stem and progenitor cells in their marker expression and activation of specific functional networks such as the transforming growth factorbeta (TGFbeta) and Notch signaling pathways. In addition, our results allow us to identify novel genes expressed in fNPCs and provide a detailed profile of fNPCs.
authors.affiliation: Stem Cell Biology Unit/Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Jingli
authors.initials: J
authors.lastname: Cai
authors.forename: Soojung
authors.initials: S
authors.lastname: Shin
authors.forename: Lynda
authors.initials: L
authors.lastname: Wright
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Daixing
authors.initials: D
authors.lastname: Zhou
authors.forename: Haipeng
authors.initials: H
authors.lastname: Xue
authors.forename: Irina
authors.initials: I
authors.lastname: Khrebtukova
authors.forename: Mark P
authors.initials: MP
authors.lastname: Mattson
authors.forename: Clive N
authors.initials: CN
authors.lastname: Svendsen
authors.forename: Mahendra S
authors.initials: MS
authors.lastname: Rao
mesh_terms: D000328:Adult; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D001921:Brain; D018797:Cell Cycle Proteins / Q000235:genetics; D002478:Cells, Cultured; D002874:Chromosome Mapping; D004707:Endoderm / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D005333:Fetus; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008648:Mesoderm / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D009414:Nerve Growth Factors / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000032:analysis; D015398:Signal Transduction / Q000235:genetics; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D005904:Glial Fibrillary Acidic Protein; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins