Puerarin induces angiogenesis in myocardium of rat with myocardial infarction.
Puerarin is a major effective ingredient extracted from the traditional Chinese medicine ge-gen (radix puerariae, RP). Recently, puerarin has been used to treat patients with coronary artery diseases (CAD). However, the mechanisms of puerarin on coronary artery diseases are still not very clear. In this study, we investigated the role of puerarin on angiogenesis in the non-ischemic and ischemic myocardium. We found that puerarin (120, 60 mg/kg, i.p.) could reduce infarct area in the heart of rat with myocardial infarction (MI). Puerarin (120 mg/kg) induced angiogenesis in the non-ischemic and ischemic myocardium, which was one of the mechanisms of curing coronary artery diseases. The gene expression or activation of vascular endothelial growth factor (VEGF), hypoxia-inducible factor 1alpha (HIF-1alpha) and endothelial nitric oxide synthase (eNOS) that correlated with angiogenesis were also induced by puerarin. From these results, we suggested that puerarin may induce therapeutic angiogenesis in myocardium of rat with MI. The mechanism may be that puerarin can induce VEGF and eNOS expression.
Zhang
Sanyin
S
State Key Laboratory in Chinese Medicine and Molecular Pharmacology, Shenzhen, China.
Chen
Shilin
S
Shen
Yingjun
Y
Yang
Dajian
D
Liu
Xijing
X
Sun-Chi
Albert Chan
AC
Xu
Hongxi
H
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology*; D009206:Myocardium / Q000473:pathology*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013268:Stimulation, Chemical; D014665:Vasodilator Agents / Q000494:pharmacology*
D007529:Isoflavones; D014665:Vasodilator Agents; D012313:RNA; C033607:puerarin