title: Increased expression levels of monocyte CCR2 and monocyte chemoattractant protein-1 in patients with diabetes mellitus.
abstract: Increased monocyte recruitment into subendothelial space in atherosclerotic lesions is one of the hallmarks of diabetic angiopathy. The aim of this study was to determine the state of peripheral blood monocytes in diabetes associated with atherosclerosis. Diabetic patients treated with/without an oral hypoglycemic agent and/or insulin for at least 1 year were recruited (n=106). We also included 24 non-diabetic control subjects. We measured serum levels of monocyte chemoattractant protein (MCP)-1, fasting plasma glucose (FPG), HbA1c, total cholesterol, triglyceride, body mass index (BMI), high sensitivity CRP (hs-CRP) and evaluated CCR2, CD36, CD68 expression on the surface of monocytes. Serum MCP-1 levels were significantly (p<0.05) higher in diabetic patients than in normal subjects. In diabetic patients, serum MCP-1 levels correlated significantly with FPG, HbA1c, triglyceride, BMI, and hs-CRP. The expression levels of CCR2, CD36, and CD68 on monocytes were significantly increased in diabetic patients and were more upregulated by MCP-1 stimulation. Our data suggest that elevated serum MCP-1 levels and increased monocyte CCR2, CD36, CD68 expression correlate with poor blood glucose control and potentially contribute to increased recruitment of monocytes to the vessel wall in diabetes mellitus.
authors.affiliation: First Department of Internal Medicine, University of Occupational and Environmental Health, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Mine
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Okada
authors.forename: Takahisa
authors.initials: T
authors.lastname: Tanikawa
authors.forename: Chie
authors.initials: C
authors.lastname: Kawahara
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tabata
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000276:immunology*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology*; D005786:Gene Expression Regulation; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D054390:Receptors, CCR2; D019707:Receptors, Chemokine / Q000378:metabolism*
chemical_list: C515334:CCR2 protein, human; D018932:Chemokine CCL2; D008077:Lipoproteins, LDL; D054390:Receptors, CCR2; D019707:Receptors, Chemokine