[Expression and characterization of transcutaneous immunization adjuvant LTB and LTK63].
BACKGROUND
To study a new kind of adjuvant: transcutaneous immunization adjuvant.


METHODS
The full length gene of Heat-labile enterotoxin (LT) was amplified from E. coli H10407. The B subunit protein LTB and the nontoxic A subunit protein LTKA were expressed by genetic engineering manipulation. After purification, they were identified with SDS-PAGE, GM1-ELISA and so on.


RESULTS
The LTB protein still persisted its biologic activity that conjugated specifically with GM1 ganglioside, and the LTK63 protein lost its toxin activity.


CONCLUSIONS
The results showed that LTB and LTK63 may be used as promising transcutaneous immunization adjuvant.
Chen
Si-yong
SY
Department of Hepatitis Virus, Institute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Yi
Yao
Y
Guo
Yu
Y
Bi
Sheng-li
SL
D000276:Adjuvants, Immunologic / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015870:Gene Expression; D005818:Genetic Engineering; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; C027538:heat-labile enterotoxin, E coli