title: [Establishment of immortalized B lymphoblast cell line from patients with chronic hepatitis B].
abstract: OBJECTIVE
  To establish immortalized B lymphoblast cell line (BLCL) from patients with chronic hepatitis B (CHB) in vitro.
  
  
  METHODS
  The peripheral blood mononuclear cells (PBMC) were isolated from the patients with CHB by routine method and incubated with EB virus (EBV) in the presence of CpG DNA motifs and cyclosporin A (CysA) for about 28 days. Morphological characteristic of the established immortalized BLCL was observed by microscope and the expression of CD19 and CD23 on cellular surface was determined by flow cytometry.
  
  
  RESULTS
  BLCL was successfully established and could be cultured and propagated for a long time in vitro.
  
  
  CONCLUSIONS
  EBV-immortalized BLCL provides a resource of target cells for further research on the cellular immunity of patients with CHB.
authors.affiliation: Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. zhangmx@fimmu.com
authors.forename: Ming-xia
authors.initials: MX
authors.lastname: Zhang
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Diao
authors.forename: Hai-tang
authors.initials: HT
authors.lastname: He
authors.forename: Jin-lin
authors.initials: JL
authors.lastname: Hou
authors.forename: Kang-xian
authors.initials: KX
authors.lastname: Luo
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000473:pathology* / Q000821:virology; D018929:Cell Culture Techniques; D002460:Cell Line; D002478:Cells, Cultured; D006509:Hepatitis B / Q000473:pathology; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000737:chemistry; D019694:Hepatitis B, Chronic / Q000473:pathology*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C439765:C19orf33 protein, human; D006513:Hepatitis B e Antigens; D009687:Nuclear Proteins; D014157:Transcription Factors