title: I222 crystal form of despentapeptide (B26-B30) insulin provides new insights into the properties of monomeric insulin.
abstract: Despentapeptide (des-B26-B30) insulin (DPI), an active modified insulin, has been crystallized in the presence of 20% acetic acid pH 2. A crystal structure analysis to 1.8 A spacing (space group I222) revealed that the DPI molecule, which is unable to make beta-strand interactions for physiological dimer formation and is apparently monomeric in solution, formed an alternative lattice-generated dimer. The formation of this dimer involved interactions between surfaces which included the B9-B19 alpha-helices (usually buried by the dimer-dimer contacts within the native hexamer). The two crystallographically independent molecules within the dimer were essentially identical and were similar in conformation to T-state insulin as seen in the T(6) insulin hexamer. An unusual feature of each molecule in the dimer was the presence of two independent conformations at the B-chain C-terminus (residues B20-B25). Both conformations were different from that of native insulin, involving a 3.5 A displacement of the B20-B23 beta-turn and a repositioning of residue PheB25 such that it made close van der Waals contact with the main body of the molecule, appearing to stabilize the B-chain C-terminus.
authors.affiliation: York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5YW, England.
authors.forename: Jean L
authors.initials: JL
authors.lastname: Whittingham
authors.forename: Zhang
authors.initials: Z
authors.lastname: Youshang
authors.forename: Lenka
authors.initials: L
authors.lastname: Záková
authors.forename: Eleanor J
authors.initials: EJ
authors.lastname: Dodson
authors.forename: Johan P
authors.initials: JP
authors.lastname: Turkenburg
authors.forename: Jens
authors.initials: J
authors.lastname: Brange
authors.forename: G Guy
authors.initials: GG
authors.lastname: Dodson
mesh_terms: D018360:Crystallography, X-Ray; D019281:Dimerization; D007328:Insulin / Q000031:analogs & derivatives* / Q000737:chemistry; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: D007328:Insulin; C007285:insulin, despentapeptide(B26-B30)-