Use of Ecotilling as an efficient SNP discovery tool to survey genetic variation in wild populations of Populus trichocarpa.
Abstract Ecotilling was used as a simple nucleotide polymorphism (SNP) discovery tool to examine DNA variation in natural populations of the western black cottonwood, Populus trichocarpa, and was found to be more efficient than sequencing for large-scale studies of genetic variation in this tree. A publicly available, live reference collection of P. trichocarpa from the University of British Columbia Botanical Garden was used in this study to survey variation in nine different genes among individuals from 41 different populations. A large amount of genetic variation was detected, but the level of variation appears to be less than in the related species, Populus tremula, based on reported statistics for that tree. Genes examined varied considerably in their level of variation, from PoptrTB1 which had a single SNP, to PoptrLFY which had more than 23 in the 1000-bp region examined. Overall nucleotide diversity, measured as (Total), was relatively low at 0.00184. Linkage disequilibrium, on the other hand, was higher than reported for some woody plant species, with mean r2 equal to 0.34. This study reveals the potential of Ecotilling as a rapid genotype discovery method to explore and utilize the large pool of genetic variation in tree species.
Gilchrist
Erin J
EJ
Department of Botany, University of British Columbia, Vancouver, BC, Canada V6T 1Z4.
Haughn
George W
GW
Ying
Cheng C
CC
Otto
Sarah P
SP
Zhuang
Jun
J
Cheung
Dorothy
D
Hamberger
Björn
B
Aboutorabi
Fariba
F
Kalynyak
Tatyana
T
Johnson
Lee
L
Bohlmann
Joerg
J
Ellis
Brian E
BE
Douglas
Carl J
CJ
Cronk
Quentin C B
QC
D002170:Canada; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D017753:Ecosystem; D017343:Genes, Plant; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D015810:Linkage Disequilibrium; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D032107:Populus / Q000145:classification / Q000235:genetics*; D014481:United States
D018744:DNA, Plant