Genetics-based methods for detection of Salmonella spp. in foods.
Genetic methods are now at the forefront of foodborne pathogen testing. The sensitivity, specificity, and inclusivity advantages offered by deoxyribonucleic acid (DNA) probe technology have driven an intense effort in methods development over the past 20 years. DNA probe-based methods for Salmonella spp. and other pathogens have progressed from time-consuming procedures involving the use of radioisotopes to simple, high throughput, automated assays. The analytical sensitivity of nucleic acid amplification technology has facilitated a reduction in analysis time by allowing enriched samples to be tested for previously undetectable quantities of analyte. This article will trace the evolution of the development of genetic methods for detection of Salmonella in foods, review the basic assay formats and their advantages and limitations, and discuss method performance characteristics and considerations for selection of methods.
Mozola
Mark A
MA
Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA. mmozola@neogen.com
D000818:Animals; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D005516:Food Microbiology; D005821:Genetic Techniques / Q000295:instrumentation*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012475:Salmonella / Q000737:chemistry* / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial