title: Enantiomeric separation using an l-RNA aptamer as chiral additive in partial-filling capillary electrophoresis.
abstract: In this paper, we report the chiral resolution of arginine using an anti-arginine l-RNA aptamer chiral selector in partial-filling CE. The effects of the capillary temperature, sample load, and aptamer plug length on the enantiomeric separation were assessed. Very high chiral resolving capability was observed at low or moderate capillary temperatures (the target peak being not detected in the separation window), whereas the practical chiral resolution was achieved only at high enough temperatures (50-60 degrees C). Over this high-temperature range, the electrophoretic behavior of the target enantiomer appeared to result from a combination of binding site heterogeneity, slow desorption kinetics, and concentration overload of aptamer binding sites. From additional thermal UV melting experiments, three RNA conformations were identified for the 50-60 degrees C temperatures. It was suggested that the presence of these different RNA conformations was a plausible source of the binding site heterogeneity.
authors.affiliation: Département de Pharmacochimie Moléculaire, UMR 5063 CNRS, ICMG FR 2607, Université Joseph Fourier, UFR de Pharmacie de Grenoble, Avenue de Verdun, 38240 Meylan, France.
authors.forename: Josephine
authors.initials: J
authors.lastname: Ruta
authors.forename: Corinne
authors.initials: C
authors.lastname: Ravelet
authors.forename: Catherine
authors.initials: C
authors.lastname: Grosset
authors.forename: Jennifer
authors.initials: J
authors.lastname: Fize
authors.forename: Anne
authors.initials: A
authors.lastname: Ravel
authors.forename: Annick
authors.initials: A
authors.lastname: Villet
authors.forename: Eric
authors.initials: E
authors.lastname: Peyrin
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001120:Arginine / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013696:Temperature; D013997:Time Factors
chemical_list: D052157:Aptamers, Nucleotide; D001120:Arginine