Kainic acid activates oxytocinergic neurons through non-nmda glutamate receptors.
The present study assessed if kainic acid activates oxytocinergic neurons and this activation is blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Dual immunohistochemistry for oxytocin and c-Fos showed that oxytocin neurons in SON and PVN express c-Fos following kainic acid administration, a significant increase when compared to the control group. Administration of CNQX prior to kainic acid caused a significant reduction. The results suggested the participation of non-NMDA receptors in the regulation of oxytocin neurons because the administration of kainic acid activates these neurons and this activation is blocked by CNQX administration.
Zehra Minbay
Fatma
F
Department of Histology and Embryology, Uludag University Faculty of Medicine, Bursa, Turkey. zminbay@uludag.edu.tr
Eyigor
Ozhan
O
Cavusoglu
Ilkin
I
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count / Q000379:methods; D004347:Drug Interactions; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D007608:Kainic Acid / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010121:Oxytocin / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000502:physiology*; D013495:Supraoptic Nucleus / Q000166:cytology
D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D016760:Proto-Oncogene Proteins c-fos; D017470:Receptors, Glutamate; D010121:Oxytocin; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D007608:Kainic Acid