title: Analysis of Indole-3-Acetic Acid Metabolites from Dalbergia dolichopetala by High Performance Liquid Chromatography-Mass Spectrometry.
abstract: A mixture of [2-(14)C(1)] and [(13)C(6)]indole-3-acetic acid was applied to the cotyledons of 6-day-germinated seeds of "jacarandá do cerrado" (Dalbergia dolichopetala) and after 8 hours the seeds were extracted. Analysis of the fractionated extract by reversed-phase high performance liquid chromatography-radiocounting revealed the presence of five radiolabeled metabolite peaks (I-V). After further purification, the individual peaks of radioactivity were analyzed by combined high performance liquid chromatography-steel filter-fast atom bombardment-mass spectrometry. The metabolite fraction V was found to contain [(14)C(1), (13)C(6)]indole-3-acetylas-partic acid and unlabeled indole-3-acetylglutamic acid. Analysis of the metabolite fraction II revealed the presence of dioxindole-3-acetylaspartic acid and putative dioxindole-3-acetylglutamic acid as well as putative benzene ring-hydroxylated derivatives of oxindole-3-acetylaspartic acid and oxindole-3-acetylglutamic acid. There was no evidence of significant incorporation of label from [2'-(14)C(1)] or [(13)C(6)]indole-3-acetic acid into any of these conjugated indoles.
authors.affiliation: Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Ostin
authors.forename: A M
authors.initials: AM
authors.lastname: Monteiro
authors.forename: A
authors.initials: A
authors.lastname: Crozier
authors.forename: E
authors.initials: E
authors.lastname: Jensen
authors.forename: G
authors.initials: G
authors.lastname: Sandberg