Enhanced excitability and suppression of A-type K+ current of pancreas-specific afferent neurons in a rat model of chronic pancreatitis.
Chronic pancreatitis (CP) is a relatively common disorder, characterized by glandular insufficiency and chronic, often intractable, pain. The mechanism of pain in CP is poorly understood. We have previously developed a model of trinitrobenzene sulphonic acid (TNBS)-induced CP that results in nociceptive sensitization in rats. This study was designed to examine changes in the excitability and alteration of voltage-gated K(+) currents of dorsal root ganglia (DRG) neurons innervating the pancreas. CP was induced in adult rats by an intraductal injection of TNBS. DRG neurons innervating the pancreas were identified by 1,1'-dioleyl-3,3,3',3-tetramethylindocarbocyanine methanesulfonate fluorescence labeling. Perforated patch-clamp recordings were made from acutely dissociated DRG neurons from control and TNBS-treated rats. Pancreas-specific DRG neurons displayed more depolarized resting potentials in TNBS-treated rats than those in controls (P < 0.02). Some neurons from the TNBS-treated group exhibited spontaneous firings. TNBS-induced CP also resulted in a dramatic reduction in rheobase (P < 0.05) and a significant increase in the number of action potentials evoked at twice rheobase (P < 0.05). Under voltage-clamp conditions, neurons from both groups exhibited transient A-type (I(A)) and sustained outward rectifier K(+) currents (I(K)). Compared with controls, the average I(A) but not the average I(K) density was significantly reduced in the TNBS-treated group (P < 0.05). The steady-state inactivation curve for I(A) was displaced by approximately 20 mV to more hyperpolarized levels after the TNBS treatment. These data suggest that TNBS treatment increases the excitability of pancreas-specific DRG neurons by suppressing I(A) density, thus identifying for the first time a specific molecular mechanism underlying chronic visceral pain and sensitization in CP.
Xu
Guang-Yin
GY
Div. of Gastroenterology and Hepatology, Dept. of Internal Medicine, Univ. of Texas Medical Branch, Galveston, Texas 77555, USA. jpasrich@utmb.edu
Winston
John H
JH
Shenoy
Mohan
M
Yin
Huaizhi
H
Pasricha
Pankaj Jay
PJ
D000200:Action Potentials; D000344:Afferent Pathways; D000818:Animals; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000187:drug effects / Q000503:physiopathology*; D015640:Ion Channel Gating; D008297:Male; D008564:Membrane Potentials; D010146:Pain / Q000139:chemically induced / Q000209:etiology / Q000503:physiopathology; D050500:Pancreatitis, Chronic / Q000139:chemically induced / Q000150:complications / Q000503:physiopathology*; D020671:Posterior Horn Cells / Q000187:drug effects / Q000503:physiopathology*; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels; D051381:Rats; D017207:Rats, Sprague-Dawley; D014302:Trinitrobenzenesulfonic Acid
D015221:Potassium Channels; D014302:Trinitrobenzenesulfonic Acid; D011188:Potassium