Fluorescence enhancement of yttrium(III)-rutin by nucleic acids in the presence of cetyltrimethylammonium bromide.
It is found that nucleic acids can enhance the fluorescence intensity of yttrium(III) (Y(3+))-rutin in presence of cetyltrimethylammonium bromide (CTMAB) system. In hexamethylenetetramine (HMTA)-HCl buffer, the maximum enhanced fluorescence is produced, with maximum excitation and emission wavelengths at 452 and 520 nm, respectively. Based on this, a new fluorimetric method of determination of nucleic acids is proposed. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 1.0 x 10(-7) to 1.0 x 10(-5)g/ml for fish sperm DNA (fsDNA), 1.0 x 10(-7) to 4.6 x 10(-6)g/ml for yeast RNA (yRNA), their detection limits (S/N=3) are 7.5 x 10(-8), 8.0 x 10(-8)g/ml, respectively. The interaction mechanism is also studied.
Wu
Xia
X
Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
Guo
Changying
C
Wang
Fei
F
Yang
Jinghe
J
Ran
Dehuan
D
Zheng
Jinhua
J
Wu
Jinbo
J
D000818:Animals; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D005399:Fishes / Q000235:genetics; D005453:Fluorescence; D008297:Male; D012313:RNA / Q000032:analysis*; D012331:RNA, Fungal / Q000032:analysis; D012431:Rutin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013094:Spermatozoa / Q000737:chemistry; D015003:Yeasts / Q000235:genetics; D015019:Yttrium / Q000737:chemistry*
D002593:Cetrimonium Compounds; D012331:RNA, Fungal; D015019:Yttrium; D012431:Rutin; D012313:RNA; D004247:DNA; D000077286:Cetrimonium