title: Proteomic analysis of prodigiosin-induced apoptosis in a breast cancer mitoxantrone-resistant (MCF-7 MR) cell line.
abstract: Prodigiosin (PG) is a bacterial, red-pigmented antibiotic with immunosuppressive and apoptotic activities. To better understand its mechanisms of action, we tried to identify proteins associated with apoptosis induced by PG. For this purpose, the variation of protein expression on exposure to apoptotic concentrations of PG was examined, by high-resolution two-dimensional gel electrophoresis (2D-E), in the MCF-7 cancer cell line resistant to mitoxantrone (MCF-7-MR). Six PG apoptosis-associated protein spots were further characterized by complementary peptide mass fingerprinting and tandem mass spectrometry data obtained on a matrix-assisted laser desorption ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometer. The proteins identified were involved in various cellular functions, including cell defence, DNA repair and cellular organization. Our data provide novel information on cell response to PG, a new apoptotic drug with interesting anticancer activity.
authors.affiliation: Department of Pathology and Experimental Therapeutic, Cancer Cell Biology Research Group, University of Barcelona, Barcelona, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Monge
authors.forename: Marta
authors.initials: M
authors.lastname: Vilaseca
authors.forename: Vanessa
authors.initials: V
authors.lastname: Soto-Cerrato
authors.forename: Beatriz
authors.initials: B
authors.lastname: Montaner
authors.forename: Ernest
authors.initials: E
authors.lastname: Giralt
authors.forename: Ricardo
authors.initials: R
authors.lastname: Pérez-Tomás
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western / Q000379:methods; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005982:Glutathione Transferase / Q000032:analysis; D006801:Humans; D053538:Keratin-18 / Q000032:analysis; D053539:Keratin-19 / Q000032:analysis; D053533:Keratin-8 / Q000032:analysis; D008942:Mitoxantrone / Q000494:pharmacology*; D010750:Phosphoproteins / Q000032:analysis; D011353:Prodigiosin / Q000302:isolation & purification / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D012269:Ribosomal Proteins / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D053538:Keratin-18; D053539:Keratin-19; D053533:Keratin-8; D010750:Phosphoproteins; D020543:Proteome; D012269:Ribosomal Proteins; C054675:ribosomal protein P0; D008942:Mitoxantrone; C483009:GSTM3 protein, human; D005982:Glutathione Transferase; D011353:Prodigiosin