title: Safety and antiulcer efficacy studies of Achillea millefolium L. after chronic treatment in Wistar rats.
abstract: Achillea millefolium L. (Asteraceae), popularly known as yarrow, has been used in folk medicine to treat complaints such as inflammation, pain, wounds, hemorrhages and gastrointestinal disturbances. The aim of the present study was to assess the safety and efficacy of the aqueous extract (AE) of the plant after chronic exposure. Indeed, the AE was effective in protecting the gastric mucosa against acute gastric lesions induced by ethanol and indomethacin and in healing chronic gastric lesions induced by acetic acid with (ED(50)=32 mg/kg, p.o.). Safety studies were performed in female and male Wistar rats treated daily with AE (0.3-1.2 g/kg, p.o./day) or vehicle (water, 10 ml/kg/day) for 28 or 90 consecutive days. Satellite groups consisted of animals sacrificed 30 days after the end of these treatments. Clinical observations, body and organ weight measurements, gross autopsy, hematology, clinical biochemical and histopathological examinations were performed. Slight changes in liver weight, cholesterol, HDL-cholesterol and glucose were observed in male and female animals. These changes were not correlated with dose or time of exposure of the animals to the AE. Overall, the results show the antiulcer potential of the aerial parts of the Achillea millefolium which is accompanied by no signs of relevant toxicity even at very long chronic exposure.
authors.affiliation: Department of Pharmacology, Universidade Federal do Paraná, Caixa Postal 19031, Centro Politécnico - Jardim das Américas, Curitiba, PR 81531-990, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Cavalcanti
authors.forename: Cristiane Hatsuko
authors.initials: CH
authors.lastname: Baggio
authors.forename: Cristina Setim
authors.initials: CS
authors.lastname: Freitas
authors.forename: Lia
authors.initials: L
authors.lastname: Rieck
authors.forename: Renato Silva
authors.initials: RS
authors.lastname: de Sousa
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Da Silva-Santos
authors.forename: Sonia
authors.initials: S
authors.lastname: Mesia-Vela
authors.forename: Maria Consuelo Andrade
authors.initials: MC
authors.lastname: Marques
mesh_terms: D031162:Achillea / Q000737:chemistry*; D000284:Administration, Oral; D000818:Animals; D000897:Anti-Ulcer Agents / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D001772:Blood Cell Count; D001777:Blood Coagulation / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D005260:Female; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000188:drug therapy*; D013997:Time Factors
chemical_list: D000897:Anti-Ulcer Agents; D010936:Plant Extracts