[Detection of expression of Th1/Th2 cytokines in jurkat cell treated with gamma-interferon by cytometric bead array].
In order to explore the value of gamma-interferon (IFN-gamma) in therapy of T lymphoid leukemia and role of cytometric bead array (CBA) method in detection of Th1/Th2 cytokines expression, the Jurkat cells were cultured for 48 hours in different concentration of IFN-gamma, the expressions of IL-2, IL-6, INF-gamma and TNF-alpha in culture supernatants were assayed by CBA method; CD25 expression was assayed by flow cytometry. The results showed that the expressions of IL-2, TNF-alpha were enhanced in INF-gamma dose-dependent way, and the expression of CD25 was also enhanced, but there was no expression of IL-6. It is concluded that Jurkat cells induced by IFN-gamma were able to express high-level of Th1 cytokine and IL-2 membrane receptor (CD25), and the CBA method can be used to exactly evaluate the dynamic change of Th1/Th2 cytokines.
Li
Wan-Hong
WH
Laboratory Analysis Center, Taizhou City Central Hospital, Taizhou 318000, China. liwanh_111@yahoo.com.cn
Wang
Ling-Li
LL
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis* / Q000494:pharmacology*; D007376:Interleukin-2 / Q000032:analysis*; D019169:Jurkat Cells; D008863:Microspheres; D012680:Sensitivity and Specificity; D018417:Th1 Cells / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma