Anti-inflammatory activity of Ailanthus altissima in ovalbumin-induced lung inflammation.
As part of an ongoing investigation to find bioactive medicinal herbs exerting anti-inflammation activity, the effect of an ethanol extract from the parts of Ailanthus altissima (Simaroubaceae) was evaluated in both in vitro and in in vivo system. The ethanol extract of A. altissima (EAa) inhibited generation of the cyclooxygenase-2 (COX-2) dependent phases of prostaglandin D2 in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner with an IC50 value of 214.6 microg/ml. However, this compound did not inhibit COX-2 protein expression up to a concentration of 400 microg/ml in the BMMC, indicating that EAa directly inhibits COX-2 activity. In addition, EAa inhibited leukotriene C4 production with an IC50 value of 25.7 microg/ml. Furthermore, this compound inhibited degranulation reaction in a dose dependent manner, with an IC50 value of 27.3 microg/ml. Ovalbumin (OVA)-sensitized mice were orally pretreated with EAa before aerosol challenges. EAa reduced the eosinophil infiltration into the airway and the eotaxin, IL-4, and IL-13 mRNA expression levels. These results suggest that the anti-inflammation activity of A. altissima in OVA-induced lung inflammation may occur in part via the down regulation of T(H)2 cytokines and eotaxin transcripts as well as the inhibition of inflammatory mediators.
Jin
Mei Hua
MH
College of Pharmacy, Yeungnam University, Gyongsan, Korea.
Yook
Jumin
J
Lee
Eunkyung
E
Lin
Chang Xin
CX
Quan
Zhejiu
Z
Son
Kun Ho
KH
Bae
Ki Hwan
KH
Kim
Hyun Pyo
HP
Kang
Sam Sik
SS
Chang
Hyeun Wook
HW
D032268:Ailanthus / Q000737:chemistry*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D016718:Arachidonic Acid / Q000378:metabolism; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000201:enzymology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis; D018076:DNA, Complementary / Q000096:biosynthesis; D004804:Eosinophils / Q000502:physiology; D005260:Female; D007958:Leukocyte Count; D008407:Mast Cells / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D011014:Pneumonia / Q000139:chemically induced* / Q000517:prevention & control*; D015230:Prostaglandin D2 / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D052246:Cyclooxygenase 2 Inhibitors; D016207:Cytokines; D018076:DNA, Complementary; D016718:Arachidonic Acid; D010047:Ovalbumin; D001094:Arachidonate 5-Lipoxygenase; D051546:Cyclooxygenase 2; D001619:beta-N-Acetylhexosaminidases; D015230:Prostaglandin D2