Identification and characterization of novel adeno-associated virus isolates in ATCC virus stocks.
Adeno-associated viruses (AAVs) depend on a helper virus for efficient replication. To identify novel AAV isolates, we screened a diverse set of virus isolates for the presence of AAV DNA. AAVs found in 10 simian adenovirus isolates showed greater than 96% homology to AAV1 and AAV6 but had distinct biological properties. Two representatives of this group, AAV(VR-195) and AAV(VR-355), were studied in more detail. While the novel AAVs had high sequence homologies and required sialic acid for cell binding and transduction, differences were observed in lectin competition, resulting in distinct tropisms in human cancer cell lines.
Schmidt
Michael
M
Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD 20892, USA.
Grot
Emmanuelle
E
Cervenka
Peter
P
Wainer
Sandra
S
Buck
Charles
C
Chiorini
John A
JA
D036022:Capsid Proteins / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D000229:Dependovirus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D012689:Sequence Homology, Nucleic Acid
D036022:Capsid Proteins; D004279:DNA, Viral; D019158:N-Acetylneuraminic Acid