Transcriptomes of human prostate cells.
BACKGROUND
The gene expression profiles of most human tissues have been studied by determining the transcriptome of whole tissue homogenates. Due to the solid composition of tissues it is difficult to study the transcriptomes of individual cell types that compose a tissue. To overcome the problem of heterogeneity we have developed a method to isolate individual cell types from whole tissue that are a source of RNA suitable for transcriptome profiling.


RESULTS
Using monoclonal antibodies specific for basal (integrin beta4), luminal secretory (dipeptidyl peptidase IV), stromal fibromuscular (integrin alpha 1), and endothelial (PECAM-1) cells, respectively, we separated the cell types of the prostate with magnetic cell sorting (MACS). Gene expression of MACS-sorted cell populations was assessed with Affymetrix GeneChips. Analysis of the data provided insight into gene expression patterns at the level of individual cell populations in the prostate.


CONCLUSIONS
In this study, we have determined the transcriptome profile of a solid tissue at the level of individual cell types. Our data will be useful for studying prostate development and cancer progression in the context of single cell populations within the organ.
Oudes
Asa J
AJ
Urology, University of Washington, Seattle, WA 98195-6510, USA. aoudes@systemsbiology.org
Campbell
Dave S
DS
Sorensen
Carrie M
CM
Walashek
Laura S
LS
True
Lawrence D
LD
Liu
Alvin Y
AY
D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002478:Cells, Cultured; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D018189:Immunomagnetic Separation; D008297:Male; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D010641:Phenotype; D011467:Prostate / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D017154:Stromal Cells / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
D019485:Bone Morphogenetic Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta