Role of 11-beta-hydroxysteroid dehydrogenase type 1 in differentiation of 3T3-L1 cells and in rats with diet-induced obesity.
OBJECTIVE
To observe the roles of 11-beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in in vitro preadipocyte differentiation and in rats with diet-induced obesity (DIO).


METHODS
Protein expression of 11beta-HSD1 in the process of 3T3-L1 cell differentiation and in various tissues of the rats were detected by Western blot analysis; expression of 11beta-HSD1 mRNA and glucocorticoid receptor (GR) and other marker genes of preadipocyte differentiation were detected by using real-time PCR.


RESULTS
Lipid droplets in 3T3-L1 cells accumulated and increased after stimulation. A dramatically elevated protein level of 11beta-HSD1, especially in the late stages of 3T3-L1 cell differentiation, was detected. The relative mRNA levels of 11beta-HSD1, GR and cell differentiation markers LPL, aP2, and FAS were upregulated, and Pref-1 was downregulated during the differentiation. In DIO rats, bodyweight, visceral adipose mass index and the protein expression of 11beta-HSD1 increased, especially in adipose tissue, brain and muscles. Serum insulin, triglyceride, total cholesterol and low-density lipoprotein cholesterol were found to be increased in DIO rats, but without any obvious changes in blood glucose or tumor necrosis factor-alpha levels.


CONCLUSIONS
11beta-HSD1 may promote preadipocyte differentiation, and may be involved in the development of obesity.
Liu
Yun
Y
Department of Pharmacology, Nanjing Medical University, Nanjing 210029, China.
Sun
Wen-Lan
WL
Sun
Yan
Y
Hu
Gang
G
Ding
Guo-Xian
GX
D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000096:biosynthesis* / Q000235:genetics; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002784:Cholesterol / Q000097:blood; D064429:Fatty Acid Synthases / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D051379:Mice; D009765:Obesity / Q000201:enzymology* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics; D014280:Triglycerides / Q000097:blood
D007328:Insulin; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D014280:Triglycerides; D002784:Cholesterol; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D064429:Fatty Acid Synthases; D008071:Lipoprotein Lipase