Alkaline polyethylene glycol-based method for direct PCR from bacteria, eukaryotic tissue samples, and whole blood.
Chomczynski
Piotr
P
Molecular Research Center, Cincinnati, OH 45212, USA. piotr@mrcgene.com
Rymaszewski
Michal
M
D000468:Alkalies / Q000737:chemistry; D000818:Animals; D001419:Bacteria / Q000235:genetics; D001774:Blood Chemical Analysis; D005591:Chemical Fractionation / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats
D000468:Alkalies; D007202:Indicators and Reagents; D011092:Polyethylene Glycols; D004247:DNA