title: Recognition of pore-forming colicin Y by its cognate immunity protein.
abstract: Construction of hybrid immunity genes between colicin U (cui) and Y (cyi) immunity genes and site-directed mutagenesis of cyi were used to identify amino-acid residues of the colicin Y immunity protein (Cyi) involved in recognition of colicin Y. These amino-acid residues were localized close to the cytoplasmic site of the Cyi transmembrane helices T3 (S104, S107, F110, A112) and T4 (A159). Mutations in cui, which converted Cui sequence to Cyi sequence in positions 104, 107, 110, 112 and 159, resulted in an immunity gene that also conferred (besides immunity to colicin U) a high degree of immunity to colicin Y.
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. dsmajs@med.muni.cz
authors.forename: David
authors.initials: D
authors.lastname: Smajs
authors.forename: Petra
authors.initials: P
authors.lastname: Matejková
authors.forename: George M
authors.initials: GM
authors.lastname: Weinstock
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000502:physiology; D003087:Colicins / Q000737:chemistry*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D003087:Colicins; C007104:colicin immunity proteins