title: Biosynthesis of White Lupin Isoflavonoids from [U-C]l-Phenylalanine and Their Release into the Culture Medium.
abstract: Pulse-labeling experiments of white lupin (Lupinus albus L.) cell cultures with [U-(14)C]l-phenylalanine for 72 h resulted in the incorporation of the radioactivity into the isoflavone aglucones, glucosides, and prenylated derivatives. Both the aglucones genistein and 2'-hydroxygenistein and their 7-O-glucosides accounted for 85% of the total isoflavonoids identified in the cultured cells and contained 35% of the radioactivity, whereas the prenylated derivatives comprised 15 and 65%, respectively. Almost 20% of the labeled isoflavones of the cellular pool was recovered from the culture medium, 90% of which were monoprenylated and diprenylated derivatives containing 80% of the radioactivity. These results clearly demonstrate the release into the culture medium of a substantial amount of the endogenously synthesized isoflavonoids, especially the prenylated derivatives.
authors.affiliation: Plant Biochemistry Laboratory, Department of Biology, Concordia University, Montréal, Québec, Canada H3G 1M8.
authors.forename: H
authors.initials: H
authors.lastname: Gagnon
authors.forename: J
authors.initials: J
authors.lastname: Seguin
authors.forename: E
authors.initials: E
authors.lastname: Bleichert
authors.forename: S
authors.initials: S
authors.lastname: Tahara
authors.forename: R K
authors.initials: RK
authors.lastname: Ibrahim