title: Binding of TmHU to single dsDNA as observed by optical tweezers.
abstract: Optical tweezers are employed to study the action of the histone-like protein from Thermotoga maritima (TmHU) on DNA at a single molecule level. Binding and disruption of TmHU to and from DNA are found to take place in discrete steps of 4-5 nm length and a net binding enthalpy of about 16kBT. This is in reasonable agreement with a microscopic model that estimates the extension of the binding sites of the protein and evaluates the energetics mainly for bending of the DNA in the course of interaction.
authors.affiliation: Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Brüderstrasse 34, D-04103 Leipzig, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Salomo
authors.forename: K
authors.initials: K
authors.lastname: Kroy
authors.forename: K
authors.initials: K
authors.lastname: Kegler
authors.forename: C
authors.initials: C
authors.lastname: Gutsche
authors.forename: M
authors.initials: M
authors.lastname: Struhalla
authors.forename: J
authors.initials: J
authors.lastname: Reinmuth
authors.forename: W
authors.initials: W
authors.lastname: Skokov
authors.forename: C
authors.initials: C
authors.lastname: Immisch
authors.forename: U
authors.initials: U
authors.lastname: Hahn
authors.forename: F
authors.initials: F
authors.lastname: Kremer
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry*; D008958:Models, Molecular; D055095:Optics and Photonics; D011485:Protein Binding; D011487:Protein Conformation; D020124:Thermotoga maritima / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; C040330:histone-like protein HU, bacteria; D004247:DNA