Multiplexed phosphoprotein analysis in immune cells.
Analyzing signaling networks in immune cells is of particular interests in diseases where treatment choices are preferentially immunomodulatory. By combining phospho-specific antibodies with multicolor flow cytometry it is possible to perform quantitative multiparameter analysis of signaling pathways within complex cell populations. Multiplexed phosphoprotein analysis will potentially incorporate environmental factors such as toxins or pathogens and genetic variability of individual patients in a step towards personalized medicine.
Gavasso
S
S
Department of Clinical Medicine, Section of Neurology, University of Bergen and Haukeland University Hospital, Norway. sonja.gavasso@helse-bergen.no
Myhr
K-M
KM
Vedeler
C
C
D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D009103:Multiple Sclerosis / Q000378:metabolism* / Q000473:pathology* / Q000628:therapy; D010750:Phosphoproteins / Q000378:metabolism*; D050794:STAT1 Transcription Factor / Q000378:metabolism; D050797:STAT4 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
D010750:Phosphoproteins; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D050797:STAT4 Transcription Factor; C494090:STAT4 protein, human