[Peptide fragment 66-77 of monocyte chemoattractant protein 1 and its retro-enantio analogue inhibits the migration of cells in vitro and in vivo].
The retro-enantio analogue of peptide 66-77 of the chemokine MCP-1 and two hexapeptide fragments 66-71 and 72-77 of the C-terminal sequence of this protein were synthesized using the Fmoc strategy of solid phase peptide synthesis. The effect of the synthetic peptides upon the MCP-1-stimulated migration of THP-1 mononuclear cells was studied in vitro. The activity of the retro-enantio analogue was found to be comparable with that of the initial peptide 66-77: both peptides inhibit the migration of monocytes and granulocytes into inflammation zones of experimental animals.
Sidorova
M V
MV
Molokoedov
A S
AS
Az'muko
A A
AA
Aref'eva
T I
TI
Melekhov
M G
MG
Kukhtina
N B
NB
Krasnikova
T L
TL
Bespalova
Zh D
ZhD
Bushuev
V N
VN
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000737:chemistry* / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006098:Granulocytes / Q000187:drug effects / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism
D018932:Chemokine CCL2; D008070:Lipopolysaccharides; D010446:Peptide Fragments