title: Molecular diagnosis of culture-negative cerebral nocardiosis due to Nocardia abscessus.
abstract: We describe a case of culture-negative cerebral nocardiosis in a 34-year-old immunocompetent man who presented multiple cerebral abscesses. All bacteriologic cultures were negative. Nocardiosis was diagnosed by using a direct genus-specific 16S rDNA amplification method, and Nocardia abscessus was identified by hsp65 sequence analysis. The patient is alive and well on imipenem and doxycycline therapy, 14 months after onset.
authors.affiliation: Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 34295 Montpellier Cedex 5, France. h-marchandin@chu-montpellier.fr
authors.forename: Hélène
authors.initials: H
authors.lastname: Marchandin
authors.forename: Aurélia
authors.initials: A
authors.lastname: Eden
authors.forename: Hélène
authors.initials: H
authors.lastname: Jean-Pierre
authors.forename: Jacques
authors.initials: J
authors.lastname: Reynes
authors.forename: Estelle
authors.initials: E
authors.lastname: Jumas-Bilak
authors.forename: Patrick
authors.initials: P
authors.lastname: Boiron
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laurent
mesh_terms: D000328:Adult; D001922:Brain Abscess / Q000175:diagnosis* / Q000382:microbiology; D005784:Gene Amplification / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009615:Nocardia / Q000145:classification / Q000235:genetics; D009617:Nocardia Infections / Q000175:diagnosis* / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014057:Tomography, X-Ray Computed
chemical_list: D012336:RNA, Ribosomal, 16S