Preventive effect of oral administration of 6-(methylsulfinyl)hexyl isothiocyanate derived from wasabi (Wasabia japonica Matsum) against pulmonary metastasis of B16-BL6 mouse melanoma cells.
OBJECTIVE
Effect of oral administration of 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC) or a 6-MITC-containing T-wasabi fraction from wasabi root (Wasabia japonica Matsum) to inhibit the macroscopic pulmonary metastasis was studied with a murine B16-BL6 melanoma model.


METHODS
Two administration routes, subcutaneous or intravenous, and two administration times, prior to or concomitant with tumor inoculation, of 6-MITC or T-wasabi against the metastatic foci formation in C57BL/6J mouse lungs were compared.


RESULTS
The number of metastasized foci per lung in either subcutaneous or intravenous injection was significantly reduced by intake of 6-MITC or a T-wasabi fraction. The maximum reduction by a T-wasabi fraction reached to 82%. Fifty-six percent of foci formation was inhibited by a 2 week-prior administration of 6-MITC (200 microM), whereas only 27% inhibition was obtained by a concomitant administration with tumor inoculation. Neither 6-MITC nor T-wasabi at tested concentrations showed any toxic effects.


CONCLUSIONS
Together with our previous results, a component of the Japanese pungent spice, wasabi appears to inhibit not only tumor cell growth but also tumor metastasis. Therefore, 6-MITC from wasabi is apparently a useful dietary candidate for controlling tumor progression.
Fuke
Yoko
Y
Department of Food Science and Human Nutrition, Tokyo Metropolitan University, Akishima, Tokyo 196-8540, Japan. fuke@tmca.ac.jp
Shinoda
Shoko
S
Nagata
Ikuko
I
Sawaki
Saeko
S
Murata
Mituyoshi
M
Ryoyama
Kazuo
K
Koizumi
Keiichi
K
Saiki
Ikuo
I
Nomura
Takahiro
T
D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017879:Isothiocyanates / Q000008:administration & dosage / Q000138:chemical synthesis / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000517:prevention & control / Q000556:secondary*; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000517:prevention & control / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology; D018517:Plant Roots; D031228:Wasabia; D023041:Xenograft Model Antitumor Assays
C505386:6-(Methylsulfinyl)hexyl isothiocyanate; D000970:Antineoplastic Agents; D017879:Isothiocyanates; D028321:Plant Preparations