Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene.
Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed that gyrBint constitutes a discriminative target sequence to differentiate between Enterobacteriaceae species. Comparison of these identifications with those obtained by phenotypic methods (Vitek 1 system and/or Rapid ID 32E; bioMérieux, Marcy l'Etoile, France) revealed discrepancies essentially with genera Citrobacter and Enterobacter. Most of the strains identified as Enterobacter cloacae by phenotypic methods were identified as Enterobacter hormaechei strains by gyrBint sequencing. The direct sequencing of gyrBint would be useful as a complementary tool in the identification of clinical Enterobacteriaceae isolates.
Delmas
Julien
J
Laboratoire de Bactériologie, Centre Hospitalo-Universitaire, 28 place Henri Dunant, 63001 Clermont-Ferrand Cedex, France. jdelmas@chu-clermontferrand.fr
Breysse
Franck
F
Devulder
Gregory
G
Flandrois
Jean-Pierre
JP
Chomarat
Monique
M
D015373:Bacterial Typing Techniques / Q000379:methods*; D027081:DNA Gyrase / Q000235:genetics*; D004755:Enterobacteriaceae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods
D027081:DNA Gyrase