Localization of neuregulin-1alpha (heregulin-alpha) and one of its receptors, ErbB-4 tyrosine kinase, in developing and adult human brain.
Using immunohistochemistry, Western blot analysis, and RT-polymerase chain reaction, we studied the distribution of neuregulin-1 splice variant alpha (NRG-1alpha) and one of its putative receptors, ErbB-4 tyrosine kinase, in human brain. In the pre- and perinatal human brain immunoreactivity was confined to numerous neurons, with the highest cell density found in cortical gray matter, hypothalamus and cerebellum. In the adult brain, single cortical gray and white matter neurons showed NRG-1alpha immunoreactivity. Occasionally, immunoreactive oligodendrocytes were observed. NRG-1alpha-expressing neurons were also found in the hypothalamus, hippocampus, basal ganglia and brain stem. Application of two antibodies recognizing alpha and beta isoforms revealed a different distribution pattern in that many cortical and hippocampal pyramidal neurons were labeled. ErbB-4 immunoreactivity was expressed in both neurons and oligodendrocytes. Our data show that NRG-1alpha expression is lower in the adult human brain than in the developing brain, and, therefore, support a role for NRG-1alpha in brain development.
Bernstein
Hans-Gert
HG
Department of Psychiatry, University of Magdeburg, Germany. Hans-Gert.Bernstein@medizin.uni-magdeburg.de
Lendeckel
Uwe
U
Bertram
Iris
I
Bukowska
Alicja
A
Kanakis
Dimitrios
D
Dobrowolny
Henrik
H
Stauch
Renate
R
Krell
Dieter
D
Mawrin
Christian
C
Budinger
Eike
E
Keilhoff
Gerburg
G
Bogerts
Bernhard
B
D000328:Adult; D017398:Alternative Splicing; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000254:growth & development* / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D005333:Fetus; D006801:Humans; D007150:Immunohistochemistry; D008251:Macaca; D008297:Male; D020890:Neuregulin-1 / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D009836:Oligodendroglia / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D066247:Receptor, ErbB-4; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
D020890:Neuregulin-1; D020033:Protein Isoforms; C587689:ERBB4 protein, human; D066246:ErbB Receptors; C587691:Erbb4 protein, rat; D066247:Receptor, ErbB-4