title: Introduction of a validation concept for a PCR-based Mycoplasma detection assay.
abstract: BACKGROUND
  Mycoplasma contamination is amongst the most frequently occurring problems associated with cell cultures. In order to meet the legal requirements (European Pharmacopoeia and FDA) for Mycoplasma testing of cell lines and therapeutics, we have developed a PCR-based method to detect mycoplasms and introduce a validation concept.
  
  
  METHODS
  The PCR assay specifically amplifies a 280-bp DNA fragment of the gene coding for the 16S rDNA. Simultaneous amplification of an artificial oligonucleotide containing primer-binding sites allowed control of the efficacy of the PCR. The validation of the PCR assay was performed with two Mycoplasma reference strains, M. orale and M. pneumoniae. The validation concept included (i) cultivation of M. orale and M. pneumoniae in medium with an indicator for bacterial metabolism, (ii) determination of the color-changing units (CCU) in repeated dilution experiments and (iii) correlation of the PCR results with CCU values.
  
  
  RESULTS
  The detection range was found to include all Mycoplasma species most commonly found in cell cultures. The analytical sensitivity of the PCR was the CCU equivalent of 100 for M. orale and M. pneumoniae. Probit analysis revealed a detection probability of 9% for a mean concentration of 1222 (935-1844) CCU/mL for M. pneumoniae and 2547 (1584-10,352) CCU/mL for M. orale.
  
  
  CONCLUSIONS
  The validation of the Mycoplasma detection assay supported PCR as an attractive diagnostic tool that will help manage the important issue of Mycoplasma contamination of cell cultures.
authors.affiliation: Institute of Transfusion Medicine and Immunology, Red Cross Blood Service of Baden-Württemberg-Hessen, University of Heidelberg, Faculty of Clinical Medicine Mannheim, Mannheim, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Bruchmüller
authors.forename: E
authors.initials: E
authors.lastname: Pirkl
authors.forename: R
authors.initials: R
authors.lastname: Herrmann
authors.forename: M
authors.initials: M
authors.lastname: Stoermer
authors.forename: H
authors.initials: H
authors.lastname: Eichler
authors.forename: H
authors.initials: H
authors.lastname: Klüter
authors.forename: P
authors.initials: P
authors.lastname: Bugert
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000592:standards*; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D015203:Reproducibility of Results; D012107:Research Design; D021161:Self-Sustained Sequence Replication; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S