Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle.
We described the development of functionalized magnetic nanoparticles (MNPs) with PEG-modification, a phospholipids micelle coating, and their use in manipulating histidine-tagged proteins. Highly monodisperse MNPs were synthesized in an organic solvent and could be phase-transferred into an aqueous solution by encapsulating the nanoparticles with a phospholipids micelle. The phospholipids micelle coating rendered the nanoparticles highly water-soluble, and the functional groups of the phospholipids coating allowed for the bioconjugation of various moieties, such as fluorescent molecules and engineered proteins. Functionalized phospholipids, such as nitrilotriacetic acid (NTA)-phospholipids, caused the MNPs to bind and allowed for manipulation of histidine-tagged proteins. Due to their high surface/volume ratio, the MNPs showed better performance (about 100 times higher) in immobilizing engineered proteins than conventional micrometer-sized beads. This demonstrates that MNPs coated with phospholipids micelle can be a versatile platform for the effective manipulation of various kinds of engineered proteins, which is very important in the field of proteomics. It is expected that a combination of MNPs with optical fluorescent molecules can find applications in bimodal (magnetic and optical) molecular imaging nanoprobes.
Lim
Yong Taik
YT
Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Lee
Kun Yeong
KY
Lee
Kwangyeol
K
Chung
Bong Hyun
BH
D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000528:radiation effects; D006639:Histidine / Q000737:chemistry* / Q000528:radiation effects; D008280:Magnetics; D008823:Micelles; D009038:Motion; D049329:Nanostructures / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure*; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure
D020099:Coated Materials, Biocompatible; D008823:Micelles; D010743:Phospholipids; D011994:Recombinant Proteins; D006639:Histidine; D009571:Nitrilotriacetic Acid