title: [The action of dexamethasone on trans epithelial electrical resistance and the expression of aquaporin-1 in immortalized trabecular cells].
abstract: OBJECTIVE
  To study the effect of dexamethasone on trans epithelial electrical resistance (TEER) and the expression of AQP-1 in immortalized trabecular cells.
  
  
  METHODS
  Immortalized human trabecular cells were cultured on PET membrane in 24 wells plate. The cells were treated with Dexamethasone in concentration of 10(-7) mol/L. After the cultured cell became confluence, TEER of the cells was detected to evaluate the resistance of outflow pathway at different time points. Western blot was used to analyze the expression of AQP-1 in the trabecular cells.
  
  
  RESULTS
  The TEER and the expression of AQP-1 were much higher in the cells treated by Dexamethasone in comparison to control without dexamethasone exposure.
  
  
  CONCLUSIONS
  The TEER and expression of AQP-1 in immortalized trabecular cells can be enhanced by Dexamethasone. AQP-1 upregulation induced by Glucocorticoid may relate with the increased resistance of outflow pathway in glaucoma.
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Ye-hong
authors.initials: YH
authors.lastname: Zhuo
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
authors.forename: Ya-lin
authors.initials: YL
authors.lastname: Huang
authors.forename: Yan-taoi
authors.initials: YT
authors.lastname: Wei
authors.forename: Yun-lan
authors.initials: YL
authors.lastname: Ling
authors.forename: Ming-ka
authors.initials: MK
authors.lastname: Lin
mesh_terms: D051398:Aquaporin 1 / Q000096:biosynthesis*; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D017097:Electric Impedance; D006801:Humans; D014129:Trabecular Meshwork / Q000187:drug effects* / Q000503:physiopathology
chemical_list: D051398:Aquaporin 1; D003907:Dexamethasone