title: Aflatoxin B1-induced DNA damage in Labeo rohita: protective effect of an antioxidant supplement, Amrita Bindu.
abstract: The present study was undertaken to investigate the effect of potent hepatocarcinogen aflatoxin B1 in adduct formation and DNA damage in Labeo rohita. Also, the salubrious efficacy of an antioxidant supplement Amrita Bindu (based on Indian system of Medicine) was investigated. Fish weighing 175-250 g were administered intraperitoneally a single dose of 100 microg aflatoxin B1/100 g body wt. and another group was given 20% solution of Amrita Bindu along with aflatoxin B1 at 100 microg/100 g body wt. On the 3rd and 6th day, the liver tissue was analyzed for aflatoxin concentration, aflatoxin-DNA adduct formation and DNA damage measured in terms of single strand breaks. The fishes administered with aflatoxin B1 showed elevated concentration of aflatoxin along with a parallel increase in the DNA adduct when compared with the controls. While the fish co-administered with Amrita Bindu showed 34% and 24% reduction in aflatoxin deposition (accumulation) and aflatoxin-DNA adduct formation respectively on the 3rd day, a further reduction by around 41% and 33% in aflatoxin deposition and DNA adduct formation respectively was observed on the 6th day. Furthermore, the increased single strand breaks (measured by alkaline single cell gel assay) and fragmentation observed in agarose gel electrophoresis in aflatoxin B1 administered fish were significantly reduced by Amrita Bindu co-administration. In conclusion, this is the first report to show aflatoxin B1-induced DNA adduct formation and DNA damage in one of the major Indian culturable fish, Labeo rohita. Also, our observations show that the antioxidant supplement, Amrita Bindu, has a potential role in ameliorating the aflatoxin B1-induced DNA damage thus suggesting its applicability in preventing the vital macromolecule DNA.
authors.affiliation: Department of Medical Biochemistry, Dr. A. L. Mudaliar Postgraduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600 113, India.
authors.forename: N
authors.initials: N
authors.lastname: Madhusudhanan
authors.forename: S N
authors.initials: SN
authors.lastname: Kavithalakshmi
authors.forename: E R B
authors.initials: ER
authors.lastname: Shanmugasundaram
authors.forename: K R
authors.initials: KR
authors.lastname: Shanmugasundaram
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002347:Carps / Q000235:genetics / Q000378:metabolism*; D020552:Comet Assay; D018736:DNA Adducts / Q000378:metabolism*; D004249:DNA Damage; D019587:Dietary Supplements; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008515:Medicine, Ayurvedic; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D013997:Time Factors
chemical_list: C515274:Amrita Bindu; D000975:Antioxidants; D018736:DNA Adducts; D009153:Mutagens; D010936:Plant Extracts; C072611:aflatoxin B1-DNA adduct; D016604:Aflatoxin B1