Peptide ligands targeting integrin alpha3beta1 in non-small cell lung cancer.
Lung cancer is one of the most common cancers and is the leading cause of cancer death. We wish to identify peptide ligands for unique cell surface receptors of non-small lung cancer with the hope of developing these ligands as diagnostic and therapeutic agents. Using the method of 'one-bead one-peptide' combinatorial chemistry, a library of random cyclic octapeptides was synthesized on polystyrene beads. This library was used to screen for peptides that promoted attachment of lung adenocarcinoma cells employing a 'cell-growth-on-bead' assay. Consensus peptide sequences of cNGXGXXc were identified. These peptides promoted cell adhesion by targeting integrin alpha3beta1 over-expressed in non-small lung cancer cells. These peptide beads can be applied to capture cancer cells in malignant pleural fluid for purpose of diagnosis of lung cancer.
Lau
Derick
D
Division of Hematology/Oncology, Department of Internal Medicine, University of California, Davis, Cancer Center and Northern California VA Health System, 4501 X Street, Sacramento, CA 95817, USA. derick.lau@ucdmc.ucdavis.edu
Guo
Linlang
L
Liu
Ruiwu
R
Marik
Jan
J
Lam
Kit
K
D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D039201:Integrin alpha3beta1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008024:Ligands; D008175:Lung Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology*; D019151:Peptide Library
D039201:Integrin alpha3beta1; D008024:Ligands; D009363:Neoplasm Proteins; D009842:Oligopeptides; D019151:Peptide Library