Identification of novel genes with prognostic value in childhood leukemia using cDNA microarray and quantitative RT-PCR.
The aim of this study was to identify genes distinctively expressed or suppressed in childhood leukemia with different prognoses, using cDNA microarray and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression levels of the selected genes by cDNA microarray were quantified in primary leukemic blasts from 44 patients (acute lymphoblastic leukemia, 28; acute myelogenous leukemia (AML), 13; transient myeloproliferative disorder, 3). The expression levels of CDKN2C, CRADD, and IGFBP-2 genes were significantly associated with the event-free survival of the patients in AML. The present results suggest that a combination of cDNA microarray and quantitative RT-PCR may be useful to identify novel genes with prognostic value in childhood AML.
Hattori
Hiroyoshi
H
Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. hhattori@pediatr.med.kyushu-u.ac.jp
Matsuzaki
Akinobu
A
Suminoe
Aiko
A
Koga
Yuhki
Y
Tashiro
Kosuke
K
Hara
Toshiro
T
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000293:Adolescent; D053420:CRADD Signaling Adaptor Protein; D053143:Caspase 2; D002648:Child; D002675:Child, Preschool; D050763:Cyclin-Dependent Kinase Inhibitor p18 / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D052138:Genes, Neoplasm; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000235:genetics; D007938:Leukemia / Q000235:genetics*; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; C493971:CDKN2C protein, human; D053420:CRADD Signaling Adaptor Protein; C104252:CRADD protein, human; D050763:Cyclin-Dependent Kinase Inhibitor p18; D018971:Insulin-Like Growth Factor Binding Protein 2; D009363:Neoplasm Proteins; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins; D017346:Protein Serine-Threonine Kinases; C506249:RIPK1 protein, human; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; C490360:CASP2 protein, human; D053143:Caspase 2; D003546:Cysteine Endopeptidases