title: [Influence of individual domains of the translation termination factor eRF1 on induction of the GTPase activity of the translation termination factor eRF3].
abstract: Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA but needs the presence of eRF1. It's known that eRF1 and eRF3 interact with each other in vitro and in vivo via their C-terminal regions. eRF1 consists of three domains - N, M, and C. In this study we examined the influence of individual domains of the human eRF1 on induction of the human eRF3 GTPase activity in the ribosome in vitro. It was shown that none of the N-, M-, C- and NM-domains induces eRF3 GTPase activity in presence of the ribosomes. MC-domain does induce GTPase activity of eRF3 but four times less efficient than full-length eRF1, therefore, MC-domain (and very likely M-domain) binds to the ribosome in the presence of eRF3. Based on these data and taking into account the data available in literature, a conclusion was drawn that the N domain of eRF1 is not essential for eRF1-dependent induction of the eRF3 GTPase activity. A working hypothesis is formulated, postulating that GTPase activity eRF3 during the translation termination is associated with the intermolecular interactions of GTP/GDP, GTPase center of the large ribosomal subunit (60S), MC-domain of eRF1, C-terminal region and GTP-binding domains of eRF3, but without participation of the N-terminal region of eRF3.
authors.forename: V I
authors.initials: VI
authors.lastname: Dubovaia
authors.forename: P M
authors.initials: PM
authors.lastname: Kolosov
authors.forename: E Z
authors.initials: EZ
authors.lastname: Alkalaeva
authors.forename: L Iu
authors.initials: LIu
authors.lastname: Frolova
authors.forename: L L
authors.initials: LL
authors.lastname: Kiselev
mesh_terms: D000818:Animals; D002474:Cell-Free System / Q000378:metabolism; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D010454:Peptide Termination Factors / Q000378:metabolism*; D010458:Peptidyl Transferases / Q000378:metabolism; D014176:Protein Biosynthesis / Q000502:physiology*; D017434:Protein Structure, Tertiary; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D012156:Reticulocytes / Q000166:cytology / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
chemical_list: C489002:ETF1 protein, human; D010454:Peptide Termination Factors; D012346:RNA, Transfer, Amino Acyl; D011994:Recombinant Proteins; C087971:peptide-chain-release factor 3; D006160:Guanosine Triphosphate; D010458:Peptidyl Transferases; D020558:GTP Phosphohydrolases