Lipase-mediated transformation of vegetable oils into biodiesel using propan-2-ol as acyl acceptor.
Propan-2-ol was used as an acyl acceptor for immobilized lipase-catalyzed preparation of biodiesel. The optimum conditions for transesterification of crude jatropha (Jatropha curcas), karanj (Pongamia pinnata) and sunflower (Helianthus annuus) oils were 10% Novozym-435 (immobilized Candida antarctica lipase B) based on oil weight, alcohol to oil molar ratio of 4:1 at 50 degrees C for 8 h. The maximum conversions achieved using propan-2-ol were 92.8, 91.7 and 93.4% from crude jatropha, karanj and sunflower oils, respectively. Reusability of the lipase was maintained over 12 repeated cycles with propan-2-ol while it reached to zero by 7(th) cycle when methanol was used as an acyl acceptor, under standard reaction conditions.
Modi
Mukesh Kumar
MK
Division of Lipid Science and Technology, Indian Institute of Chemical Technology, Hyderabad, India.
Reddy
J R C
JR
Rao
B V S K
BV
Prasad
R B N
RB
D019840:2-Propanol / Q000378:metabolism; D001709:Biotechnology; D001711:Biotransformation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D004951:Esterification; D005656:Fungal Proteins; D005742:Gasoline; D031286:Jatropha; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism*; D052596:Pongamia; D000074242:Sunflower Oil
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005742:Gasoline; D010938:Plant Oils; D000074242:Sunflower Oil; D008049:Lipase; C431349:lipase B, Candida antarctica; D019840:2-Propanol; D000432:Methanol