title: [Secretory expression of sCD14 in Pichia pastoris and its LPS binding activity].
abstract: OBJECTIVE
  To achieve secretory expression of mature sCD14 gene in Pichia pastoris and to analyze its LPS binding activity.
  
  
  METHODS
  sCD14 gene was cloned into yeast expression vector pPIC9K. Recombinant plasmid was lineared by Sac I and transformed into Pichia pastoris GS115. Positive integrated clones were screened and expressed by methanol inducing.
  
  
  RESULTS
  The mature sCD14 was expressed in BMMY with pH 6.5 after optimization of inducing expression condition. Western blot and ELISA data revealed recombinant products combined with specific antibody. The results from FACS showed recombinant product had biological activity of binding with LPS.
  
  
  CONCLUSIONS
  The recombinant mature sCD14 molecules with favorable LPS-binding activity could be used for further study on its structure and functions.
authors.affiliation: Faculty of Stomatology, Capital University of Medical Sciences, Beijing 100050, China. bjbenxiang@21cn.net
authors.forename: Ben-xiang
authors.initials: BX
authors.lastname: Hou
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D012634:Bodily Secretions; D015870:Gene Expression; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics
chemical_list: D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides