title: Ovol1 regulates the growth arrest of embryonic epidermal progenitor cells and represses c-myc transcription.
abstract: Transcriptional control plays a key role in regulating epidermal proliferation and differentiation. Although ample information has been obtained on how epidermal homeostasis is controlled in adult skin, less is known about the control of proliferation/differentiation of epidermal stem/progenitor cells in the developing embryo. Ovol1, encoding a zinc finger protein homologous to Drosophila melanogaster Ovo, is expressed in embryonic epidermal progenitor cells that are transiting from proliferation to terminal differentiation. In this study, we demonstrate a function for Ovol1 in interfollicular epidermal development. In its absence, developing epidermis fails to properly restrict the proliferative potential of progenitor cells, and cultured keratinocytes fail to efficiently undergo growth arrest in response to extrinsic growth-inhibitory signals. We present molecular evidence that c-myc expression is up-regulated in Ovol1-deficient suprabasal cells and that Ovol1 represses c-myc transcription by directly binding to its promoter. Collectively, our findings indicate that Ovol1 is required for proliferation exit of committed epidermal progenitor cells and identify c-myc as an Ovol1 target.
authors.affiliation: Department of Biological Chemistry, University of California, Irvine, 92697, USA.
authors.forename: Mahalakshmi
authors.initials: M
authors.lastname: Nair
authors.forename: Andy
authors.initials: A
authors.lastname: Teng
authors.forename: Virginia
authors.initials: V
authors.lastname: Bilanchone
authors.forename: Anshu
authors.initials: A
authors.lastname: Agrawal
authors.forename: Baoan
authors.initials: B
authors.lastname: Li
authors.forename: Xing
authors.initials: X
authors.lastname: Dai
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology*; D015536:Down-Regulation; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D055291:Salivary alpha-Amylases; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; C490318:Ovo1 protein, mouse; D014157:Transcription Factors; C524799:Amy1 protein, mouse; D055291:Salivary alpha-Amylases