Development of a murine model of chronic rhinosinusitis.
OBJECTIVE
The aim of this study was to develop a mouse model of chronic eosinophilic rhinosinusitis.


METHODS
Mice were sensitized to Aspergillis fumigatus (Af) extract by intraperitoneal injection. The animals subsequently received nasal challenges with Af extract 3 times per week for 12 weeks. Sinonasal complexes were studied histologically by the study otolaryngologists and pathologists to characterize the inflammatory response.


METHODS
Animal care facility at an academic institution.


RESULTS
A chronic eosinophilic inflammatory response was evoked in all study animals. Statistical analysis was performed for inflammation, secretory cell hyperplasia, mast cells, and eosinophils. There were very significant differences (P<0.0005) between control and study mice in all categories.


CONCLUSIONS
Prolonged nasal challenge of Af extract creates an inflammatory response in murine nasal mucosa that mimics human chronic eosinophilic rhinosinusitis.


CONCLUSIONS
A murine model for chronic rhinosinusitis is reported that may facilitate future investigations into disease pathophysiology.


METHODS
B-2.
Lindsay
Robin
R
National Naval Medical Center, and the Uniformed Services University of the Health Sciences, Bethesda, Maryland 20889-5612, USA. rwlindsay@bethesda.med.navy.mil
Slaughter
Tiffani
T
Britton-Webb
Joy
J
Mog
Steven R
SR
Conran
Rich
R
Tadros
Monica
M
Earl
Natalie
N
Fox
David
D
Roberts
John
J
Bolger
William E
WE
D000281:Administration, Intranasal; D000818:Animals; D000946:Antigens, Fungal / Q000008:administration & dosage / Q000633:toxicity; D001232:Aspergillus fumigatus / Q000276:immunology; D002908:Chronic Disease; D004195:Disease Models, Animal; D018450:Disease Progression; D004802:Eosinophilia / Q000209:etiology / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D051379:Mice; D008807:Mice, Inbred BALB C; D012220:Rhinitis / Q000209:etiology* / Q000473:pathology; D012852:Sinusitis / Q000209:etiology* / Q000473:pathology
D000946:Antigens, Fungal