Protective effects of ethanolic neem leaf extract on DMBA-induced genotoxicity and oxidative stress in mice.
We evaluated the effects of pretreatment with ethanolic neem leaf extract on 7,12-dimethylbenz[a]anthracene (DMBA)-induced genotoxicity and oxidative stress in male Swiss albino mice. The frequency of bone marrow micronuclei, the extent of hepatic lipid peroxidation and the status of antioxidants-reduced glutathione (GSH), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were used as intermediate biomarkers of chemoprotection. In DMBA-treated mice, the increases in micronuclei and lipid peroxides were accompanied by compromised antioxidant defenses. Pretreatment with ethanolic neem leaf extract (200 mg/kg body weight) significantly reduced DMBA-induced micronuclei and lipid peroxides and enhanced GSH-dependent antioxidant activities. The results of the present study suggest that ethanolic neem leaf extract exerts protective effects against DMBA-induced genotoxicity and oxidative stress by enhancing the antioxidant status.
Subapriya
Rajamanickam
R
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India.
Kumaraguruparan
Ramasamy
R
Abraham
Suresh K
SK
Nagini
Siddavaram
S
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D031601:Azadirachta; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves
D016587:Antimutagenic Agents; D000975:Antioxidants; D009153:Mutagens; D010936:Plant Extracts; D015127:9,10-Dimethyl-1,2-benzanthracene