title: Mycoflora and multimycotoxin detection in corn silage: experimental study.
abstract: Agricultural activities involve the use of crop preservation such as "trench-type" silo, which can sometimes be contaminated by fungi. To investigate the exposure of livestock and farm workers to fungal spores and mycotoxins, a multimycotoxin analysis method has been developed. Six mycotoxins (aflatoxin B1, citrinin, deoxynivalenol, gliotoxin, ochratoxin A, and zearalenone) were quantified by high-performance liquid chromatography coupled to mass spectrometry after solid-phase extraction. An experimental study of fungal species and mycotoxins was conducted in corn silage (Normandy, France) during 9 months of monitoring. The results indicated the recurrence of around 20 different species, with some of them being potentially toxigenic fungi such as Aspergillus fumigatus, Aspergillus parasiticus, Fusarium verticillioides, and Monascus ruber, and the detection of aflatoxin B1 (4-34 ppb), citrinin (4-25 ppb), zearalenone (23-41 ppb), and deoxynivalenol (100-213 ppb). This suggested a possible chronic exposure to low levels of mycotoxins.
authors.affiliation: Groupe Régional d'Etudes sur le Cancer, GRECAN-EA 1772, Université de Caen Basse-Normandie, Centre François Baclesse, B.P. 5026, 14076 Caen Cedex 05, France. d.garon@baclesse.fr
authors.forename: David
authors.initials: D
authors.lastname: Garon
authors.forename: Estelle
authors.initials: E
authors.lastname: Richard
authors.forename: Lucile
authors.initials: L
authors.lastname: Sage
authors.forename: Valerie
authors.initials: V
authors.lastname: Bouchart
authors.forename: Didier
authors.initials: D
authors.lastname: Pottier
authors.forename: Pierre
authors.initials: P
authors.lastname: Lebailly
mesh_terms: D001230:Aspergillus / Q000302:isolation & purification; D001232:Aspergillus fumigatus / Q000302:isolation & purification; D005658:Fungi / Q000302:isolation & purification*; D005670:Fusarium / Q000302:isolation & purification; D032741:Monascus / Q000302:isolation & purification; D009183:Mycotoxins / Q000032:analysis*; D012820:Silage / Q000032:analysis* / Q000382:microbiology*; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology*
chemical_list: D009183:Mycotoxins