Myosin light chain kinase colocalizes with nonmuscle myosin IIB in myofibril precursors and sarcomeric Z-lines of cardiomyocytes.
Myosin light chain kinase (MLCK) is a key regulator of various forms of cell motility involving actin and myosin II. MLCK is widely present in vertebrate tissues including the myocardium. However, the role of MLCK in cardiomyocyte function is not known. Previous attempts to gain insight into possible roles and identify potential molecular partners were disappointing and equivocal due to cross reactivity of early antibodies with striated muscle MLCK, which has a different genetic locus and a divergent amino acid sequence from the above mentioned enzyme. Using an immunofluorescence approach and a panel of antibodies directed against MLCK, cytoskeletal, and sarcomeric proteins, we localized MLCK to myofibril precursors and Z-lines of sarcomeres in embryonic and adult cardiomyocytes. The same structures contained nonmuscle myosin IIB implicating this protein as a possible target of MLCK. Our results suggest a role for MLCK in cardiomyocyte differentiation and contraction through regulation of nonmuscle myosin IIB.
Dudnakova
T V
TV
Laboratory of Cell Motility, Russian Cardiology Research Center, Moscow, Russia.
Stepanova
O V
OV
Dergilev
K V
KV
Chadin
A V
AV
Shekhonin
B V
BV
Watterson
D M
DM
Shirinsky
V P
VP
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002642:Chick Embryo; D006352:Heart Ventricles / Q000166:cytology; D032383:Myocytes, Cardiac / Q000378:metabolism*; D009210:Myofibrils / Q000737:chemistry* / Q000378:metabolism; D009219:Myosin-Light-Chain Kinase / Q000235:genetics / Q000378:metabolism*; D024501:Nonmuscle Myosin Type IIB / Q000378:metabolism*; D012518:Sarcomeres / Q000737:chemistry* / Q000378:metabolism
D009219:Myosin-Light-Chain Kinase; D024501:Nonmuscle Myosin Type IIB