Determination of chloramphenicol residue in chicken tissues by immunoaffinity chromatography cleanup and gas chromatography with a microcell electron capture detector.
A rapid and sensitive gas chromatography (GC) method was developed to detect chloramphenicol in chicken tissues. The extracted samples were cleaned up using the immunoaffinity column prepared by coupling antichloramphenicol monoclonal antibody with cyanogen bromide-activated Sepharose 4B. The dynamic column capacity of chloramphenicol was 3265 ng/mL gel. The eluate was evaporated to dryness, and residues were derivatized and determined by GC with a microcell electron capture detector. Average recoveries were 86.6 to 96.9% for chicken muscle and 74.3 to 96.1% for chicken liver. The limit of quantitation of the method was 0.05 ng/g for chicken muscle and 0.1 ng/g for chicken liver.
Zhang
Suxia
S
China Agricultural University, College of Veterinary Medicine, Department of Pharmacology and Toxicology, Beijing 100094, People's Republic of China.
Zhou
Jinhui
J
Shen
Jianzhong
J
Ding
Shuangyang
S
Li
Jiancheng
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002138:Calibration; D002645:Chickens / Q000378:metabolism*; D002701:Chloramphenicol / Q000032:analysis*; D002846:Chromatography, Affinity; D002849:Chromatography, Gas; D004797:Enzyme-Linked Immunosorbent Assay; D007163:Immunosorbent Techniques; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D002701:Chloramphenicol