title: Extracellular matrix remodeling and organization in developing and diseased aortic valves.
abstract: Heart valve disease is an important cause of morbidity and mortality worldwide. Little is known about valve disease pathogenesis, but increasing evidence implicates a genetic basis for valve disease, suggesting a developmental origin. Although the cellular and molecular processes involved in early valvulogenesis have been well described, less is known about the regulation of valve extracellular matrix (ECM) organization and valvular interstitial cell (VIC) distribution that characterize the mature valve structure. Histochemistry, immunohistochemistry, and electron microscopy were used to examine ECM organization, VIC distribution, and cell proliferation during late valvulogenesis in chicken and mouse. In mature valves, ECM organization is conserved across species, and developmental studies demonstrate that ECM stratification begins during late embryonic cusp remodeling and continues into postnatal life. Cell proliferation decreases concomitant with ECM stratification and VIC compartmentalization. Explanted, stenotic bicuspid aortic valves (BAVs) from pediatric patients were also examined. The diseased valves exhibited disruption of the highly organized ECM and VIC distribution seen in normal valves. Cusps from diseased valves were thickened with increased and disorganized collagens and proteoglycans, decreased and fragmented elastic fibers, and cellular disarray without calcification or cell proliferation. Taken together, these studies show that normal valve development is characterized by spatiotemporal coordination of ECM organization and VIC compartmentalization and that these developmental processes are disrupted in pediatric patients with diseased BAVs.
authors.affiliation: Division of Cardiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229-3039, USA.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Hinton
authors.forename: Joy
authors.initials: J
authors.lastname: Lincoln
authors.forename: Gail H
authors.initials: GH
authors.lastname: Deutsch
authors.forename: Hanna
authors.initials: H
authors.lastname: Osinska
authors.forename: Peter B
authors.initials: PB
authors.lastname: Manning
authors.forename: D Woodrow
authors.initials: DW
authors.lastname: Benson
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Yutzey
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001021:Aortic Valve / Q000196:embryology* / Q000254:growth & development / Q000473:pathology / Q000648:ultrastructure; D001024:Aortic Valve Stenosis / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D002452:Cell Count; D049109:Cell Proliferation; D002645:Chickens; D002648:Child; D004622:Embryo, Mammalian / Q000378:metabolism; D004625:Embryo, Nonmammalian; D047108:Embryonic Development; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D051379:Mice; D011817:Rabbits; D012756:Sheep