The neural tube is required to maintain primary segmentation in the sclerotome.
Primary segmentation in vertebrates is considered to be an intrinsic property of the presomitic paraxial mesoderm controlled by a number of interconnected oscillating signals. Re-segmentation, in contrast, has been shown to depend on signals from the axial structures. Here we report the requirement of the neural tube for maintenance but not formation of primary segmentation in chick embryos. Unilateral removal of the neural tube, next to the anterior presomitic mesoderm, caused disturbed development of the neural arches and the spinous processes. But already 24 h postsurgery, the sclerotome showed loss of primary segmentation in the craniocaudal axis. Cells strongly expressing twist and not showing any segmentation were located dorsomedially between the remaining left half of the neural tube and the right side dermomyotome, which frequently was truncated medially.
Colbjørn Larsen
Karen
K
Institute of Anatomy and Cell Biology, Department of Molecular Embryology, University of Freiburg, Freiburg, Germany.
Fuchtbauer
Ernst-Martin
EM
Brand-Saberi
Beate
B
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000648:ultrastructure; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology* / Q000648:ultrastructure; D002642:Chick Embryo; D019170:Somites / Q000166:cytology / Q000648:ultrastructure*