Entamoeba histolytica: inflammatory process during amoebic liver abscess formation involves cyclooxygenase-2 expression in macrophages and trophozoites.
It has been demonstrated that expression of cyclooxygenase-2 (COX-2) isoform is induced by Entamoeba histolytica in macrophages and polymorphonuclear cells during amoebic liver abscess (ALA) formation in hamsters. Trophozoites present in the lesion were also positive for COX-2 signal. However, no cross reactivity of the anti-COX-2 antibody with protein extract of cultivated trophozoites was found. To clarify if trophozoites are involved in PGE(2) production during ALA development, COX-2 expression was detected by in situ hybridization and RT-PCR in liver tissue from intrahepatically infected hamsters. COX-2 mRNA was in polymorphonuclear cells since 4h postinfection, and subsequently, local macrophages expressed COX-2 mRNA in a similar way. Additionally, a positive signal for COX-2 mRNA expression was detected in E. histolytica trophozoites, suggesting that, in vivo, parasite COX expression may be an important mechanism to promote inflammation.
Gutiérrez-Alarcón
A
A
Laboratorio de Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Apdo. Postal 1542-C, Chihuahua, Chih., Mexico.
Moguel-Torres
M
M
Mata-Leyva
O
O
Cuellar-Nevárez
G
G
Siqueiros-Cendón
T
T
Erosa
G
G
Ramos-Martínez
E
E
Talamás-Rohana
P
P
Sánchez-Ramírez
B
B
D000818:Animals; D006224:Cricetinae; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics; D015342:DNA Probes / Q000592:standards; D015232:Dinoprostone / Q000096:biosynthesis; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006790:Host-Parasite Interactions; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007249:Inflammation / Q000201:enzymology / Q000469:parasitology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology / Q000469:parasitology / Q000473:pathology; D008101:Liver Abscess, Amebic / Q000201:enzymology / Q000469:parasitology* / Q000473:pathology; D008264:Macrophages / Q000201:enzymology / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D053058:Trophozoites / Q000201:enzymology
D015342:DNA Probes; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D015232:Dinoprostone