Surface contraction and expansion waves correlated with differentiation in axolotl embryos--I. Prolegomenon and differentiation during invagination through the blastopore, as shown by the fate map.
We have discovered a series of expansion and contraction, solitary waves that correlate with discrete steps of differentiation in the urodele amphibian axolotl embryo (Ambystoma mexicanum). Here we examine in detail the proposition that the blastopore is a set of differentiation waves. We superimposed the image of the axolotl fate map onto our digitized video images of normal gastrulation and matched the fate map to pigmentation irregularities on the embryo. We were then able to track the invagination of the fate map by tracking the variegated pigmentation on several embryos as gastrulation proceeded. We show a particular expansion and contraction wave sequence for every tissue in the blastula stage fate map and can now explain precisely why the fate map has the shape it does and its relationship to the embryo at subsequent stages. Each tissue can be assigned a differentiation code and placed on a hierarchical, binary differentiation tree.
Björklund
N K
NK
Department of Chemistry, University of Manitoba, Winnipeg, Canada R3T 2N2.
Gordon
R
R
D000558:Ambystoma mexicanum / Q000196:embryology*; D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology*; D005775:Gastrula / Q000502:physiology*