title: Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus.
abstract: SARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future.
authors.affiliation: The Center for Emerging Infectious, Li Ka-Shing Medical Institute, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
authors.forename: Ming-Liang
authors.initials: ML
authors.lastname: He
authors.forename: Bo-jian
authors.initials: BJ
authors.lastname: Zheng
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Kin-Ling
authors.initials: KL
authors.lastname: Wong
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Huang
authors.forename: Marie C
authors.initials: MC
authors.lastname: Lin
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Kwok Y
authors.initials: KY
authors.lastname: Yuen
authors.forename: Joseph J Y
authors.initials: JJ
authors.lastname: Sung
authors.forename: Hsiang-fu
authors.initials: HF
authors.lastname: Kung
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D017931:DNA Primers; D007700:Kinetics; D008253:Macaca mulatta; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D017931:DNA Primers; D034741:RNA, Small Interfering; D014764:Viral Proteins