title: Comparison of Vibrio harveyi strains isolated from shrimp farms and from culture collection in terms of toxicity and antibiotic resistance.
abstract: Vibrio harveyi strains isolated from shrimp farms (wild strains) were compared with those from culture collections in terms of minimum inhibitory concentration (MIC) and toxicity. Wild strains had higher MIC values for four antibiotics (kanamycin, carbenicillin, oxytetracycline and ampicillin) and also showed higher toxicity compared with culture collection strains. Vibrio harveyi with the lowest antibacterial resistance was chosen to test if a gradual increase in antibiotic concentration and frequent subculture would enhance its antibiotic resistance. Results showed that V. harveyi was able to develop resistance to oxytetracycline. The MIC value was 250 times higher compared with the MIC before subculturing. Moreover, the V. harveyi strain developed slightly higher toxicity. Therefore, it is possible that there is a relationship between antibiotic resistance and toxicity in V. harveyi.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nakayama
authors.forename: Emi
authors.initials: E
authors.lastname: Ito
authors.forename: Nakao
authors.initials: N
authors.lastname: Nomura
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Nomura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D046508:Culture Techniques; D024901:Drug Resistance, Multiple, Bacterial; D008826:Microbial Sensitivity Tests; D033561:Penaeidae / Q000382:microbiology*; D014733:Vibrio / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity