Uniform adherent neural progenitor populations from rhesus embryonic stem cells.
Rhesus and human embryonic stem cells (ESCs) are similar, making rhesus ESCs an appropriate preclinical allograft model for refining stem cell therapies. Use of rhesus ESC-derived neural progenitors (NPs) in preclinical applications will be enhanced if the neural derivation process is scalable and free from contaminating ESCs or nonneural cells. In this study, we have quantified temporal gene expression changes of rhesus ESC differentiated to uniform NPs using simple feeder-free adherent cultures. NPs exhibited a significant up-regulation of neural-specific genes and a downregulation of pluripotency genes. Additionally, expression of Hu, MAP2, and Tuj1, shows that NPs can form post-mitotic neurons. This study represents a simple and scalable means of producing adherent primate NPs for preclinical testing of neural cell-based therapy.
Tibbitts
Deanne
D
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA.
Rao
Raj R
RR
Shin
Soojung
S
West
Franklin D
FD
Stice
Steven L
SL
D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005353:Fibronectins / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression / Q000235:genetics; D020869:Gene Expression Profiling; D006028:Glycosphingolipids / Q000032:analysis; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D008253:Macaca mulatta; D009419:Nerve Tissue Proteins / Q000032:analysis; D064231:Nestin; D009474:Neurons / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000032:analysis; D055746:Stage-Specific Embryonic Antigens; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics
D015815:Cell Adhesion Molecules; D005353:Fibronectins; D006028:Glycosphingolipids; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D050814:Octamer Transcription Factor-3; D055746:Stage-Specific Embryonic Antigens; D014157:Transcription Factors; C066584:stage-specific embryonic antigen-4; D016222:Fibroblast Growth Factor 2