Diagnostic value of an ELISA using a recombinant 54-kDa species-specific protein from Chlamydia pneumoniae.
The aim of this study was to evaluate a 54-kDa recombinant protein encoded by the CPn0980 gene for use in a Chlamydia pneumoniae-specific ELISA. The ability of this affinity-purified protein to detect C. pneumoniae-specific antibodies was evaluated with a panel of 105 serum samples from 62 patients with community-acquired pneumonia. The results of this assay were compared with those obtained with a direct PCR-based detection assay and an outer-membrane complex-based immunoassay. The 54-kDa protein induced specific antibodies following infection of humans, and the recombinant 54-kDa ELISA detected anti-C. pneumoniae IgG and/or IgM antibodies with a sensitivity of 66.7% and a specificity of 79.2% compared with the direct PCR-based detection assay.
Sueur
J-M
JM
Laboratoire Départemental de la Somme, Amiens, France.
Beaumont
K
K
Cabioch
T
T
Orfila
J
J
Betsou
F
F
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D015153:Blotting, Western; D023521:Chlamydophila Infections / Q000097:blood* / Q000175:diagnosis / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D017714:Community-Acquired Infections / Q000097:blood / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D018410:Pneumonia, Bacterial / Q000097:blood* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry; D016415:Sequence Alignment
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins