title: Potent modulation of the voltage-gated sodium channel Nav1.7 by OD1, a toxin from the scorpion Odonthobuthus doriae.
abstract: Voltage-gated sodium channels are essential for the propagation of action potentials in nociceptive neurons. Nav1.7 is found in peripheral sensory and sympathetic neurons and involved in short-term and inflammatory pain. Nav1.8 and Nav1.3 are major players in nociception and neuropathic pain, respectively. In our effort to identify isoform-specific and high-affinity ligands for these channels, we investigated the effects of OD1, a scorpion toxin isolated from the venom of the scorpion Odonthobuthus doriae. Nav1.3, Nav1.7, and Nav1.8 channels were coexpressed with beta1-subunits in Xenopus laevis oocytes. Na+ currents were recorded with the two-electrode voltage-clamp technique. OD1 modulates Nav1.7 at low nanomolar concentrations: 1) fast inactivation is dramatically impaired, with an EC50 value of 4.5 nM; 2) OD1 substantially increases the peak current at all voltages; and 3) OD1 induces a substantial persistent current. Nav1.8 was not affected by concentrations up to 2 microM, whereas Nav1.3 was sensitive only to concentrations higher than 100 nM. OD1 impairs the inactivation process of Nav1.3 with an EC50 value of 1127 nM. Finally, the effects of OD1 were compared with a classic alpha-toxin, AahII from Androctonus australis Hector and a classic alpha-like toxin, BmK M1 from Buthus martensii Karsch. At a concentration of 50 nM, both toxins affected Nav1.7. Nav1.3 was sensitive to AahII but not to BmK M1, whereas Nav1.8 was affected by neither toxin. In conclusion, the present study shows that the scorpion toxin OD1 is a potent modulator of Nav1.7, with a unique selectivity pattern.
authors.affiliation: Laboratory of Toxicology, University of Leuven, Onderwijs and Navorsing II, Herestraat 49 - Box 922, B-3000 Leuven, Belgium.
authors.forename: Chantal
authors.initials: C
authors.lastname: Maertens
authors.forename: Eva
authors.initials: E
authors.lastname: Cuypers
authors.forename: Mehriar
authors.initials: M
authors.lastname: Amininasab
authors.forename: Amir
authors.initials: A
authors.lastname: Jalali
authors.forename: Hossein
authors.initials: H
authors.lastname: Vatanpour
authors.forename: Jan
authors.initials: J
authors.lastname: Tytgat
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D019476:Insect Proteins / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D018408:Patch-Clamp Techniques; D010446:Peptide Fragments / Q000494:pharmacology; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology; D012604:Scorpion Venoms / Q000494:pharmacology*; D012605:Scorpions; D015222:Sodium Channels / Q000235:genetics / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: C403888:BmK M1 neurotoxin; D019476:Insect Proteins; C514165:OD1 toxin, Odonthobuthus doriae; D010446:Peptide Fragments; D020033:Protein Isoforms; D012604:Scorpion Venoms; D015222:Sodium Channels; C054662:scorpion toxin II, Androctonus (19-28)