Expression of COX-2 and hsp72 in peritoneal macrophages after an acute ochratoxin A treatment in mice.
Ochratoxin A (OTA) is a secondary fungal metabolite produced by Aspergillus and Penicillium strains that elicits a broad spectrum of toxicological effects in animals and man. A single oral OTA administration (10 mg/kg) in mice induced after 24 h oxidative damage and polymorphonuclear leukocyte (PMN) infiltration in parenchymal organs. In fact, OTA treatment increased lipid peroxidation (via malondialdehyde formation) in kidney and liver and PMN accumulation in duodenum, as shown by myeloperoxidase activity. Following in vivo OTA treatment an increase of cyclooxygenase-2 and of heat shock protein 72 expression was evidenced in peritoneal macrophage lysates by Western blot. That OTA modulates these proteins involved in the inflammatory process indicates that the mycotoxin is able to activate immune cells. This study suggests that the oxidative stress, the neutrophil accumulation in parenchymal tissues and the modulation of inflammatory parameters in peritoneal macrophages induced by OTA are involved in its toxicity, and represent early events related to several aspects of OTA mycotoxicosis.
Ferrante
Maria Carmela
MC
Department of Pathology and Animal Health, University of Naples Federico II, Naples, Italy.
Bilancione
Marcella
M
Raso
Giuseppina Mattace
GM
Esposito
Emanuela
E
Iacono
Anna
A
Zaccaroni
Annalisa
A
Meli
Rosaria
R
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D009793:Ochratoxins / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism
D050884:HSP72 Heat-Shock Proteins; D009793:Ochratoxins; C025589:ochratoxin A; D008315:Malondialdehyde; D009195:Peroxidase; D051546:Cyclooxygenase 2