Assay of adenosine 5-triphosphate sulfurylase by pyrophosphate exchange.
The sulfate-dependent pyrophosphate exchange reaction has been re-examined and confirmed. Standard assay conditions for measuring ATP sulfurylase by sulfate-dependent pyrophosphate exchange are described and some properties of the enzyme (measured in crude dialyzed extracts) are reported. This method has many advantages over the well established molybdate method.
Shaw
W H
WH
Botany Department, La Trobe University, Bundoora, Vic. 3083, Australia.
Anderson
J W
JW