Proteomic analysis of differential protein expression in early process of pancreatic regeneration in pancreatectomized rats.
OBJECTIVE
A broad-range proteomic approach was applied to investigate the complexity of the mechanisms involved in pancreatic regeneration for identification of new targets of diabetes treatment and potential markers of pancreatic stem cells.


METHODS
A regeneration pancreatic model was induced by 90% partial pancreatectomy (Px) in rats. Changes in the protein expression in regenerating rat pancreas on the third day after Px, as compared with rats that received sham surgery, were analyzed by using 2-D gel electrophoresis (2-DE), mass spectrometry (MS), and mass fingerprinting.


RESULTS
2-DE revealed 91 spots with at least 1.5-fold increases in expression at 3 d after pancreatectomy and 53 differentially expressed proteins that were identified by peptide mass fingerprinting (PMF). These included cell growth-related, lipid and energy metabolism-related, protein and amino acid metabolism-related proteins, and signal transduction proteins. Vimentin, CK8, L-plastin, hnRNP A2/B1, and AGAT are associated with embryogenesis and cell differentiation, and may be new potential pancreatic stem cells markers.


CONCLUSIONS
The proteome profiling technique provided a broad-based and effective approach for the rapid assimilation and identification of adaptive protein changes during pancreas regeneration induced by pancreatectomy. Our data clarify the global proteome during the pancreatic proliferation and differentiation processes, which is important for better understanding of pancreatic regeneration and for discovering of protein biomarkers for pancreatic stem cells.
Yang
Ming
M
Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Liu
Wei
W
Wang
Chun-You
CY
Liu
Tao
T
Zhou
Feng
F
Tao
Jing
J
Wang
Yang
Y
Li
Ming-Tao
MT
D000579:Amidinotransferases / Q000032:analysis; D000818:Animals; D017305:Annexin A1 / Q000032:analysis; D002454:Cell Differentiation; D049109:Cell Proliferation; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000032:analysis; D053533:Keratin-8 / Q000032:analysis; D048370:MAP Kinase Kinase 2 / Q000032:analysis; D008297:Male; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D010179:Pancreas / Q000378:metabolism / Q000502:physiology*; D010180:Pancreatectomy; D010449:Peptide Mapping / Q000379:methods; D010750:Phosphoproteins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014746:Vimentin / Q000032:analysis
D017305:Annexin A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D053533:Keratin-8; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D010750:Phosphoproteins; D011506:Proteins; D014746:Vimentin; C026742:plastin; D000579:Amidinotransferases; C020681:glycine amidinotransferase; D048370:MAP Kinase Kinase 2