Two cases of sucrose-fermenting Vibrio vulnificus infection in which 16S rDNA sequencing was useful for diagnosis.
Vibrio vulnificus is a Gram-negative bacterium which is associated with severe infections in humans. We experienced two cases of sucrose-fermenting V. vulnificus infection. The causative agents in both cases were unidentifiable by conventional identification systems because of their unique characteristics, and sequencing of 16S rDNA was found to be useful for diagnosis.
Nagao
Miki
M
Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan. mnagao@kuhp.kyoto-u.ac.jp
Shimizu
Yuko
Y
Kawada
Yoshiyasu
Y
Baba
Hisashi
H
Yamada
Keiko
K
Torii
Keizo
K
Ohta
Michio
M
D000368:Aged; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry*; D005285:Fermentation; D006801:Humans; D008297:Male; D008875:Middle Aged; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013395:Sucrose / Q000378:metabolism*; D014735:Vibrio Infections / Q000175:diagnosis* / Q000382:microbiology*; D041261:Vibrio vulnificus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D013395:Sucrose