title: Characterization of the Legionella anisa population structure by pulsed-field gel electrophoresis.
abstract: We analysed 38 French isolates of Legionella anisa by means of pulsed-field gel electrophoresis (PFGE) with single or double digestion. Double digestion was more discriminatory than single digestion, and can thus be useful for epidemiological studies of L. anisa. Several isolates from different parts of France clustered together on the basis of their PFGE patterns (similarity cutoff of 80%), suggesting that the L. anisa population structure is homogenous or that a few clones of L. anisa strains have spread widely in France.
authors.affiliation: Centre National de référence des Legionella, Laboratoire de Bactériologie INSERM E-0230, Faculté de Médecine, Lyon, France.
authors.forename: Mongi
authors.initials: M
authors.lastname: Akermi
authors.forename: Anne
authors.initials: A
authors.lastname: Doleans
authors.forename: Françoise
authors.initials: F
authors.lastname: Forey
authors.forename: Monique
authors.initials: M
authors.lastname: Reyrolle
authors.forename: Helene
authors.initials: H
authors.lastname: Meugnier
authors.forename: Jean
authors.initials: J
authors.lastname: Freney
authors.forename: François
authors.initials: F
authors.lastname: Vandenesch
authors.forename: Jerome
authors.initials: J
authors.lastname: Etienne
authors.forename: Sophie
authors.initials: S
authors.lastname: Jarraud
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D005602:France; D007875:Legionella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D015183:Restriction Mapping