The influence of methyl jasmonate (JA-Me) and B-glucosidase on induction of resistance mechanisms of strawberry against two-spotted spider mite (Tetranychus urticae Koch.).
The goal of the research was to study the influence of methyl jasmonate (JA-Me) and beta-glucosidase treatments on fecundity and preference to infestation and oviposition of two-spotted spider mite feeding on strawberry. The experiments were conducted in laboratory conditions on leaves of Aga and Kent cultivars. Leaves were treated with: a. solution of 0.1% JA-Me in 0.05% Triton X-100 (by spraying); b. beta-glucosidase dissolved in 0.1 M citrate buffer at pH 6 (by petiole); c. 0.05% solution of the Triton X-100 (by spraying); d. 0.1 M citrate buffer at pH 6 (by petiole). In the no-choice test, application of JA-Me on leaves of strawberry caused reducing of number of eggs laid during three days of the experiment. In the choice test, which was carried out for determination of non-preference mechanism of resistance, there was a statistically significant lower number of mites on leaves treated with JA-Me compared to leaves treated with other compounds as well as to non-treated leaves after 24 hours from solutions application. Moreover, at the same experiment, females of two-spotted spider mite laid the least number of eggs on leaves treated with JA-Me. Analysis conducted using liquid chromatography method, revealed increase of the level of phenolic compounds like chlorogenic acid and rutin on leaves treated with JA-Me. Thus, it appears that JA-Me may be involved in antybiosis or non-preference mechanisms of resistance of strawberry to two-spotted spider mite.
Warabieda
W
W
Research Institute of Pomology and Floriculture, Pomologiczna 18 Str, PL-96-100 Skierniewice, Poland.
Miszczak
A
A
Olszak
R W
RW
D000085:Acetates / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology*; D005298:Fertility / Q000187:drug effects; D031985:Fragaria / Q000201:enzymology* / Q000469:parasitology*; D006863:Hydrogen-Ion Concentration; D007113:Immunity, Innate; D010058:Oviposition / Q000187:drug effects; D054883:Oxylipins; D010572:Pest Control, Biological / Q000379:methods; D010935:Plant Diseases / Q000469:parasitology; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000469:parasitology; D040102:Tetranychidae / Q000254:growth & development / Q000472:pathogenicity*; D001617:beta-Glucosidase / Q000378:metabolism / Q000494:pharmacology*
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; C072239:methyl jasmonate; D001617:beta-Glucosidase