[Histopathology analysis of microemboli after thrombolysis].
OBJECTIVE
To observe the changes in fibrin of microemboli in micro-embolism soon after thrombolysis, in order to provide a theoretical basis for micro-thrombolytic therapy.


METHODS
Thirty adult male SD rats weighing (180+/-10)g were randomized into five groups: 5, 30, 60 and 90 minutes groups after micro-thromboembolization and the control group. Rat cremasteric artery was embolized with photochemical method, and micro-thrombi were produced after thrombolysis by staphylokinase. The levels of tissue plasminogen activator (t-PA) and tissue plasminogen activator inhibitor (PAI) were immunohistochemically determined, and taken as the markers of fibrin dissolution.


RESULTS
Fibrin was observed in pathologic sections as well as fibrinolytic markers t-PA and PAI in immunohistochemical sections. The levels of t-PA were found to be reduced while that of PAI increased gradually with passage of time.


CONCLUSIONS
Fibrin is one of the main constituents of microthrombi soon after thrombolysis. The content of fibrous emboli increases after microembolization with the passage of time. Thrombolysis plays an important role in the treatment of "no reflow phenomenon".
Zhu
Hai-yan
HY
Emergency Department, General Hospital of PLA, Beijing 100853, China. zhuhy301@163.com
Shen
Hong
H
Liu
Yu-ying
YY
Zhang
Wei
W
Zhao
Xiu-mei
XM
D000818:Animals; D004195:Disease Models, Animal; D005337:Fibrin / Q000378:metabolism*; D005342:Fibrinolysis; D005343:Fibrinolytic Agents / Q000627:therapeutic use; D008297:Male; D015849:Plasminogen Inactivators / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013923:Thromboembolism / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D015912:Thrombolytic Therapy; D010959:Tissue Plasminogen Activator / Q000378:metabolism
D005343:Fibrinolytic Agents; D015849:Plasminogen Inactivators; D005337:Fibrin; D010959:Tissue Plasminogen Activator