title: Desulfovibrio alkalitolerans sp. nov., a novel alkalitolerant, sulphate-reducing bacterium isolated from district heating water.
abstract: A novel alkalitolerant, sulphate-reducing bacterium (strain RT2T) was isolated from alkaline district heating water. Strain RT2T was a motile vibrio (0.5-0.8 microm wide and 1.4-1.9 microm long) and grew at pH 6.9-9.9 (optimum at pH 9.0-9.4) and at 16-47 degrees C (optimum at 43 degrees C). The genomic DNA G+C content was 64.7 mol%. A limited number of compounds were used as electron donors with sulphate as electron acceptor, including lactate, pyruvate, formate and hydrogen/acetate. Sulphite and thiosulphate also served as electron acceptors. Based on physiological and genotypic properties, the isolate was considered to represent a novel species of the genus Desulfovibrio, for which the name Desulfovibrio alkalitolerans sp. nov. is proposed. The type strain is RT2T (=DSM 16529T=JCM 12612T). The strain is the first alkali-tolerant member of the genus Desulfovibrio to be described.
authors.affiliation: Department of Microbiology, Institute of Biological Sciences, University of Aarhus, Denmark.
authors.forename: Lone
authors.initials: L
authors.lastname: Abildgaard
authors.affiliation: Department of Microbiology, Institute of Biological Sciences, University of Aarhus, Denmark.
authors.forename: Marie Bank
authors.initials: MB
authors.lastname: Nielsen
authors.affiliation: Department of Microbiology, Institute of Biological Sciences, University of Aarhus, Denmark.
authors.forename: Kasper Urup
authors.initials: KU
authors.lastname: Kjeldsen
authors.affiliation: Department of Microbiology, Institute of Biological Sciences, University of Aarhus, Denmark.
authors.forename: Kjeld
authors.initials: K
authors.lastname: Ingvorsen
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000468:Alkalies / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003718:Denmark; D003901:Desulfovibrio / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D005561:Formates / Q000378:metabolism; D020459:Genes, rRNA; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D009068:Movement; D010084:Oxidation-Reduction; D010802:Phylogeny; D019289:Pyruvic Acid / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013431:Sulfates / Q000378:metabolism*; D013447:Sulfites / Q000378:metabolism; D013696:Temperature; D014871:Water Microbiology
chemical_list: D000468:Alkalies; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005561:Formates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D013447:Sulfites; C030544:formic acid; D019344:Lactic Acid; D006859:Hydrogen; D019289:Pyruvic Acid; D019342:Acetic Acid