[To study the expression of dendritic cell phenotypic antigen in cervical lymph nodes in hypopharyngeal carcinoma].
OBJECTIVE
Dendritic cells express many cell phenotypic antigens, they have different special function in the process of antigen present. The purpose of this study was to determine some DC phenotypic antigen in cervical lymphnodes in hypopharyngeal carcinoma and evaluate the relationship between the DC phenotypic antigen expression in hypopharyngeal carcinoma metastasis and prognosis.


METHODS
Envision HIS method was performed to detect the cell surface markers S-100, CD1a and CD83. 144 cervical lymph nodes obtained from 37 cases of hypopharyngeal carcinoma were evaluated. Furthermore, the relationship between CD45RO+ T lymphocytes and dendritic cells was observed.


RESULTS
The number of S-100+ DCs, CD1a+ DCs and CD83+ DCs in the cases of lymphnode metastasis were fewer than those in non-lymphnode metastasis. There is a significant metastasis difference in statistics between lymphnode metastasis groupe and non-lymphnode metastasis group. The number of CD83+ DCs located at cancerous nest area were fewer than those at peritumor area in lymphnode metastasis groupe. There is a significant difference in statistics between them. The number of CD83+ DCs in the cases of survival group were more than those in obituary group. CD45RO+ T cells mainly distribute around CD83+ DCs.


CONCLUSIONS
The expression of dendritic cells phenotypic antigens were significant difference in different areas in lymphnodes, may play difference role in tumor immunity against hypopharyngeal carcinoma. Dendritic cells phenotypic antigens were the important markers to predict the prognosis of the patients with hypopharyngeal carcinoma.
Li
Xiaoyan
X
Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiaotong University, First People's Hospital, China.
Nakashima
Tadashi
T
Dong
Pin
P
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009333:Neck