Task-based imaging of colon cancer in the Apc(Min/+) mouse model.
Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The Apc(Min/+) genetic mouse model of colon cancer was compared with wild-type mice. In addition, a special diet was used for the task of studying the origins of a 680 nm autofluorescent signal that was previously observed in colon. The study found close agreement among each of the modalities and with histology. All four modalities were capable of identifying diseased tissue accurately. The OCT and LSCM images provided complementary structural information about the tissue, while the autofluorescence signal measured by LIF and LSCM provided biochemical information. OCT and LIF were performed in vivo and nondestructively, while the LSCM and histology required extraction of the tissue. The magnitude of the 680 nm signal correlates with chlorophyll content in the mouse diet, suggesting that the autofluorescent compound is a dietary metabolite.
McNally
James B
JB
University of Arizona, Tuscon 85721-0104, USA. james.mcnally@gmail.com
Kirkpatrick
Nathaniel D
ND
Hariri
Lida P
LP
Tumlinson
Alexandre R
AR
Besselsen
David G
DG
Gerner
Eugene W
EW
Utzinger
Urs
U
Barton
Jennifer K
JK
D025601:Adenomatous Polyposis Coli Protein / Q000172:deficiency*; D000818:Animals; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018511:Systems Integration; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods
D025601:Adenomatous Polyposis Coli Protein