Caveolin-3-anchored microdomains at the rabbit sarcoplasmic reticulum membranes.
It is generally believed that sphingomyelin- and cholesterol-enriched microdomains can be isolated as detergent-resistant membranes (DRMs) from plasma membrane and organelle membranes. Here, we describe the isolation and characterization of microdomains from sarcoplasmic reticulum (SR) membranes. These SR-derived detergent-resistant membranes (SR-DRMs) enriched in sphingomyelin and cholesterol have a low buoyant density. Immunofluorescence microscopy of SR membranes shows the presence of caveolin-3 in the SR, known as a marker protein of caveolae at plasma membrane. We also demonstrated that significant amount of SERCAs together with caveolin-3 associates with SR-DRMs and are fully functional. Depletion of cholesterol caused the disruption of SR-DRMs.
Li
Chunlei
C
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China.
Duan
Wenjuan
W
Yang
Fuyu
F
Zhang
Xujia
X
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000032:analysis; D051244:Caveolin 3 / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology; D002784:Cholesterol / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D021962:Membrane Microdomains / Q000737:chemistry* / Q000201:enzymology; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000737:chemistry* / Q000201:enzymology; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
D051244:Caveolin 3; D003902:Detergents; D002784:Cholesterol; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases