Ultrastructure of intraradicular dentin after irrigation with BioPure MTAD. II. The consequence of obturation with an epoxy resin-based sealer.
In addition to an initial report on the creation of thick demineralized collagen matrices in root dentin that were irrigated with BioPure MTAD or EDTA as final rinses for 5 minutes, this study examined the effect of a 2-minute irrigation time of these final irrigants on the extent of demineralization of intact intraradicular dentin and on interfacial nanoleakage after obturation with AH Plus and gutta-percha using warm vertical compaction. Transmission electron microscopy confirmed that the reduction in irrigation time did not compromise the smear layer removing capacity of these two irrigants when 1.3% NaOCl was employed as the initial rinse. BioPure MTAD created 5 to 6 microm thick demineralized dentin matrices, while 17% EDTA produced 1 to 2 microm thick matrices that were suboptimally infiltrated by the epoxy resin-based sealer, as manifested by the occurrence of silver tracer deposits within the hybrid layers.
Tay
Franklin R
FR
Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, 30912, USA.
Hosoya
Yumiko
Y
Loushine
Robert J
RJ
Pashley
David H
DH
Weller
R Norman
RN
Low
Danny C Y
DC
D002614:Chelating Agents / Q000009:adverse effects; D019343:Citric Acid / Q000009:adverse effects; D003763:Dental Leakage / Q000139:chemically induced; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure*; D003806:Dentin Permeability / Q000187:drug effects*; D004318:Doxycycline / Q000009:adverse effects; D004492:Edetic Acid / Q000009:adverse effects; D004853:Epoxy Resins; D006801:Humans; D046529:Microscopy, Electron, Transmission; D011136:Polysorbates / Q000009:adverse effects; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants / Q000009:adverse effects*; D012389:Root Canal Obturation / Q000379:methods; D016369:Smear Layer; D017001:Tooth Demineralization / Q000139:chemically induced*
D002614:Chelating Agents; D004853:Epoxy Resins; C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; C534916:epoxy resin-based root canal sealer; D019343:Citric Acid; D004492:Edetic Acid; D004318:Doxycycline