title: Monoclonal anti-mouse laminin antibodies: AL-1 reacts with laminin alpha1 chain, AL-2 with laminin beta1 chain, and AL-4 with the coiled-coil domain of laminin beta1 chain.
abstract: We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-alpha1beta1gamma1 (laminin-111), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and immunostainings we present refined epitope maps for these three laminin monoclonals. AL-1 reacted, as predicted with laminin alpha1 chain. AL-4 has also been marketed as an alpha1 chain specific probe, but we show here that AL-4 detects mouse laminin beta1 chain, in the distal part of the coiled-coil region. AL-2 was predicted to react with all three chains near the cross-region, but seems to primarily react with laminin beta1 chain.
authors.affiliation: Department of Experimental Medical Science, BMC B12, Lund University, Lund, Sweden. Susanne.Scheele@med.lu.se
authors.forename: Susanne
authors.initials: S
authors.lastname: Schéele
authors.forename: Takako
authors.initials: T
authors.lastname: Sasaki
authors.forename: Anna
authors.initials: A
authors.lastname: Arnal-Estapé
authors.forename: Madeleine
authors.initials: M
authors.lastname: Durbeej
authors.forename: Peter
authors.initials: P
authors.lastname: Ekblom
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000918:Antibody Specificity; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism; D007797:Laminin / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D018482:Muscle, Skeletal / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; C478221:LAMB1 protein, human; C513277:Lamb1 protein, mouse; D007797:Laminin; C084098:laminin A