Analysis of serum granulysin in patients with hematopoietic stem-cell transplantation: its usefulness as a marker of graft-versus-host reaction.
Granulysin is a newly identified CTL/NK cell-related cytotoxic protein, which is secreted in both constitutive and Ca-dependent manner. To evaluate its significance in stem-cell transplantation (SCT), serum granulysin was measured by newly established ELISA method in 26 patients undergoing SCT (21 allogeneic and 5 autologous). In the allogeneic SCT, granulysin was transiently increased in 3 weeks, which was not observed in autologous SCT. In acute GVHD, serum granulysin was markedly increased and correlated with the severity of GVHD. Elevation of granulysin was not necessarily associated with increase of sIL2R or IFN-gamma. In vitro, allospecific T cells released granulysin in an allo-specific manner, and it was correlated with allo-specific cytotoxic activity. These results indicate that increased release of granulysin presents alloreactivity and serum granulysin is useful as a marker of GVHD in SCT.
Nagasawa
Masayuki
M
Department of Pediatrics and Developmental Biology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. mnagasawa.ped@tmd.ac.jp
Isoda
Takeshi
T
Itoh
Sukeyuki
S
Kajiwara
Michiko
M
Morio
Tomohiro
T
Shimizu
Norio
N
Ogawa
Kazuyuki
K
Nagata
Kinya
K
Nakamura
Masataka
M
Mizutani
Shuki
S
D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006086:Graft vs Host Disease / Q000097:blood* / Q000276:immunology; D006087:Graft vs Host Reaction / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D011237:Predictive Value of Tests; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; C075005:GNLY protein, human