Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli.
Allophycocyanin (APC) is the main core component of phycobilisome found in blue-green algae. The apo-allophycocyanin and its subunits were expressed in Escherichia coli and their antioxidant properties were evaluated using deoxyribose assay. The result showed that both recombinant allophycocyanin fused with maltose binding protein (MBP) tag and 6 x His-tag and their alpha or beta subunits can scavenge hydroxyl radicals successfully, and the separated alpha or beta subunits had a higher inhibition effect on hydroxyl radicals than that when they combined together. The scavenging effects increased with the increasing concentration. These results clearly suggested that apo-allophycocyanin is involved in the antioxidant and radical scavenging activity of phycocyanin, and the antioxidant activity may be partially responsible to the anti-tumor effect of the recombinant allophycocyanin.
Ge
Baosheng
B
Institute of Oceanology, Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, PR China.
Qin
Song
S
Han
Lu
L
Lin
Fan
F
Ren
Yuhong
Y
D000970:Antineoplastic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004735:Energy Transfer; D004926:Escherichia coli / Q000235:genetics; D016166:Free Radical Scavengers / Q000494:pharmacology; D005798:Genes, Bacterial; D018462:Photobiology; D010798:Phycocyanin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D046940:Synechococcus / Q000235:genetics / Q000378:metabolism
D000970:Antineoplastic Agents; D000975:Antioxidants; D004269:DNA, Bacterial; D016166:Free Radical Scavengers; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; C002497:allophycocyanin; D010798:Phycocyanin