Bacteroides finegoldii sp. nov., isolated from human faeces.
Two strains, 199T and 176, of Gram-negative, anaerobic, rod-shaped, non-spore-forming bacteria were isolated during studies of microbiota in human faeces using polyamine-deficient medium. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolated strains belonged to the genus Bacteroides. The G+C content (42.4-43.0 mol%) and major fatty acid composition (anteiso-C15:0, 31.8-36.2 %) supported the affiliation of the two strains to the genus Bacteroides. 16S rRNA gene sequence similarities with their closest neighbours, Bacteroides thetaiotaomicron and Bacteroides ovatus, ranged from 94.6 to 96.5 %. DNA-DNA hybridization and phenotypic analysis showed that the two strains belonged to the same species (DNA-DNA relatedness of 89 %) and were distinct from their close relatives, B. thetaiotaomicron and B. ovatus (<23 % DNA-DNA relatedness). On the basis of these data, a novel species of the genus Bacteroides, Bacteroides finegoldii sp. nov., is proposed, comprising the designated type strain 199T (=JCM 13345T=DSM 17565T) and strain 176 (=JCM 13346).
Bakir
Mohammad Abdul
MA
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Kitahara
Maki
M
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Sakamoto
Mitsuo
M
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Matsumoto
Mitsuharu
M
Laboratory of Dairy Science and Technology, Kyodo Milk Industry Co. Ltd, Hinode, Tokyo 190-0182, Japan.
Benno
Yoshimi
Y
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
D000328:Adult; D001439:Bacteroides / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001442:Bacteroides Infections / Q000382:microbiology; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S