Inhibitory effect of the saponins derived from roots of Platycodon grandiflorum on carrageenan-induced inflammation.
Previous studies have reported that the saponins isolated from the roots of Platycodon grandiflorum A. DC (Campanulaceae), Changkil saponins (CKS), inhibited cyclooxygenase-2 (COX-2) expression in cultured lipopolysaccharide-activated macrophages. The aim of this presented study was to confirm the anti-inflammatory effects of CKS by examining their effect on the inflammatory response induced by carrageenan in a rat by using an acute air pouch inflammation model. CKS significantly reduced the levels of the inflammatory process markers in the air pouch, such as the volume of exudates, the amount of protein and the number of leukocytes and neutrophils. The levels of TNF-alpha and prostaglandin E2 (PGE2) were also markedly lower in the air pouch of the CKS-treated animals than in the controls. An immunoblot analysis showed that CKS reduced the COX-2 expression level in the exudate cells. In addition, CKS significantly reduced the paw edema induced by carrageenan and also markedly reduced the level of PGE2 production in the inflamed paw. These results suggest that CKS had significant anti-inflammatory effects in vivo.
Kim
Ji Young
JY
Department of Pharmacy, College of Pharmacy, Research Center for Proteineous Materials, Chosun University, Kwangju, South Korea.
Hwang
Yong Pil
YP
Kim
Dong Hee
DH
Han
Eun Hee
EH
Chung
Young Chul
YC
Roh
Seong Hwan
SH
Jeong
Hye Gwang
HG
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology*; D002452:Cell Count; D002851:Chromatography, High Pressure Liquid; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005121:Extremities / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D018517:Plant Roots / Q000737:chemistry*; D031238:Platycodon / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000494:pharmacology*
D018836:Inflammation Mediators; D012503:Saponins; D002351:Carrageenan; D051546:Cyclooxygenase 2