Redox enzymes immobilized on electrodes with solution cosubstrates. General procedure for simulation of time-resolved catalytic responses.
In view of the existing and potential applications of electrochemical enzymatic catalysis with redox enzymes immobilized on the electrode surface in biosensors, a numerical calculation procedure for simulating their cyclic voltammetric responses is presented. It is applicable to systems involving a redox cosubstrate in solution. The cosubstrates, substrates, products, and inhibitors are assumed to diffuse linearly (planar electrode) between the electrode and the solution. The reactions in which the various forms of the immobilized enzyme participate may be as numerous and intricate as required by the simulation with no other restriction than the computing time. They may, at will, follow or not follow Michaelis-Menten kinetics. Slow charge-transfer cosubstrates are treated in the framework of Butler-Volmer kinetic law.
Andrieux
Claude P
CP
Laboratoire d'Electrochimie Moléculaire, Université de Paris 7-Denis Diderot, 2 place Jussieu, 75251 Paris Cedex 05, France.
Limoges
Benoît
B
Marchal
Damien
D
Savéant
Jean-Michel
JM
D002384:Catalysis; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007700:Kinetics; D010084:Oxidation-Reduction; D012996:Solutions / Q000737:chemistry; D013997:Time Factors
D004800:Enzymes, Immobilized; D012996:Solutions; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase