Kinetics of US28 gene expression during active human cytomegalovirus infection in lung-transplant recipients.
Targeting viral proteins early during infection may limit exacerbation of human cytomegalovirus infection. The viral chemokine-receptor homologue US28 interferes with leukocyte trafficking and, possibly, viral replication. Because US28 molecules are abundant on the surface of infected cells, this homologue is a potential target for antiviral therapy. To assess the relationship between US28 and disease activity, we measured, by quantitative reverse-transcription polymerase chain reaction, the levels of US28 and immediate-early (IE) 1 gene transcripts in the blood of lung-transplant recipients. We found that, during primary and secondary infection, the IE1 and US28 genes have early transcription kinetics and are expressed at similar levels. This may render US28 an attractive target for antiviral therapy.
Boomker
Jasper M
JM
Department of Pathology and Laboratory Medicine, Medical Biology Section, University Medical Center Groningen, Groningen, The Netherlands.
Verschuuren
Erik A M
EA
Brinker
Marja G L
MG
de Leij
Lou F M H
LF
The
T Hauw
TH
Harmsen
Martin C
MC
D000328:Adult; D000956:Antigens, Viral / Q000097:blood; D003586:Cytomegalovirus Infections / Q000821:virology*; D015870:Gene Expression; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D007700:Kinetics; D016040:Lung Transplantation; D008875:Middle Aged; D010750:Phosphoproteins / Q000097:blood; D012333:RNA, Messenger / Q000097:blood; D012367:RNA, Viral / Q000097:blood; D019707:Receptors, Chemokine / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014763:Viral Matrix Proteins / Q000097:blood; D014764:Viral Proteins / Q000235:genetics*
D000956:Antigens, Viral; C088645:IE1 protein, cytomegalovirus; D017874:Immediate-Early Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D019707:Receptors, Chemokine; C411191:US28 receptor, Cytomegalovirus; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C065418:cytomegalovirus matrix protein 65kDa