title: Micronuclei induction and chromosomal aberrations in Rattus norvegicus by chloroacetic acid and chlorobenzene.
abstract: Chloroacetic acid (CAA) and chlorobenzene (CB) have been evaluated for in vivo mutagenic potential in Rattus norvegicus, employing the following criteria : (i) chromosomal aberrations (CAs) such as breaks, gaps, exchanges, rings, and multiple aberrations and (ii) micronuclei (MN) induction. Three sublethal doses, 0.008, 0.01, and 0.012 mg/g b. wt. of CAA and 0.75, 1.0, and 1.25 mg/g b. wt. of rat of CB were administered and the bone marrow cells evaluated in each of the three treated groups at 12, 24, and 48 h, respectively. Mean MN frequencies of 4.40+/-0.2 and 5.42+/-0.3, obtained respectively for CAA and CB. The higher induction of MN by CAA and CB was dose- and time-dependent. Most significant impact (P<0.05) for either of the compounds was observed at 24h post administration, when the recorded mean frequency of CAs was maximum for CAA (4.33+/-0.6) as well as for CB (4.66+/-0.5).
authors.affiliation: Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Faisal Siddiqui
authors.forename: Riaz
authors.initials: R
authors.lastname: Ahmad
authors.forename: Waseem
authors.initials: W
authors.lastname: Ahmad
authors.forename: Absar-ul
authors.initials: AU
authors.lastname: Hasnain
mesh_terms: D000085:Acetates / Q000633:toxicity*; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D002722:Chlorobenzenes / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000139:chemically induced; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D013997:Time Factors
chemical_list: D000085:Acetates; D002722:Chlorobenzenes; D004785:Environmental Pollutants; D009153:Mutagens; C006972:chloroacetic acid; C031294:chlorobenzene