title: Preventive effect of oral administration of 6-(methylsulfinyl)hexyl isothiocyanate derived from wasabi (Wasabia japonica Matsum) against pulmonary metastasis of B16-BL6 mouse melanoma cells.
abstract: OBJECTIVE
  Effect of oral administration of 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC) or a 6-MITC-containing T-wasabi fraction from wasabi root (Wasabia japonica Matsum) to inhibit the macroscopic pulmonary metastasis was studied with a murine B16-BL6 melanoma model.
  
  
  METHODS
  Two administration routes, subcutaneous or intravenous, and two administration times, prior to or concomitant with tumor inoculation, of 6-MITC or T-wasabi against the metastatic foci formation in C57BL/6J mouse lungs were compared.
  
  
  RESULTS
  The number of metastasized foci per lung in either subcutaneous or intravenous injection was significantly reduced by intake of 6-MITC or a T-wasabi fraction. The maximum reduction by a T-wasabi fraction reached to 82%. Fifty-six percent of foci formation was inhibited by a 2 week-prior administration of 6-MITC (200 microM), whereas only 27% inhibition was obtained by a concomitant administration with tumor inoculation. Neither 6-MITC nor T-wasabi at tested concentrations showed any toxic effects.
  
  
  CONCLUSIONS
  Together with our previous results, a component of the Japanese pungent spice, wasabi appears to inhibit not only tumor cell growth but also tumor metastasis. Therefore, 6-MITC from wasabi is apparently a useful dietary candidate for controlling tumor progression.
authors.affiliation: Department of Food Science and Human Nutrition, Tokyo Metropolitan University, Akishima, Tokyo 196-8540, Japan. fuke@tmca.ac.jp
authors.forename: Yoko
authors.initials: Y
authors.lastname: Fuke
authors.forename: Shoko
authors.initials: S
authors.lastname: Shinoda
authors.forename: Ikuko
authors.initials: I
authors.lastname: Nagata
authors.forename: Saeko
authors.initials: S
authors.lastname: Sawaki
authors.forename: Mituyoshi
authors.initials: M
authors.lastname: Murata
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ryoyama
authors.forename: Keiichi
authors.initials: K
authors.lastname: Koizumi
authors.forename: Ikuo
authors.initials: I
authors.lastname: Saiki
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nomura
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017879:Isothiocyanates / Q000008:administration & dosage / Q000138:chemical synthesis / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000517:prevention & control / Q000556:secondary*; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000517:prevention & control / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology; D018517:Plant Roots; D031228:Wasabia; D023041:Xenograft Model Antitumor Assays
chemical_list: C505386:6-(Methylsulfinyl)hexyl isothiocyanate; D000970:Antineoplastic Agents; D017879:Isothiocyanates; D028321:Plant Preparations