Differential expression of a new isoform of DLG2 in renal oncocytoma.
BACKGROUND
Renal oncocytoma, a benign tumour of the kidney, may pose a differential diagnostic problem due to overlapping phenotype with chromophobe renal cell carcinoma or other types of renal cell tumours. Therefore, identification of molecular markers would be of great value for molecular diagnostics of this tumour type.


METHODS
In the current study we applied various techniques, including Affymetrix microarray hybridization and semiquantitative RT-PCR, to identify genes expressed differentially in renal oncocytomas. Subsequently, we used RACE and Northern blot hybridization to characterize the potential candidates for molecular diagnosis.


RESULTS
We have identified new isoform of DLG2 gene, which contains 3'-end exons of the known DLG2 gene along with the hypothetical gene FLJ37266. The new isoform is specifically upregulated in renal oncocytoma, whereas the known DLG2 gene is downregulated in this type of kidney tumour.


CONCLUSIONS
The new isoform of DLG2 is the promising candidate gene for molecular differential diagnostics of renal oncocytoma.
Zubakov
Dmitry
D
Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany.
Stupar
Zorica
Z
Kovacs
Gyula
G
D018249:Adenoma, Oxyphilic / Q000175:diagnosis* / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000175:diagnosis / Q000235:genetics; D003937:Diagnosis, Differential; D015536:Down-Regulation; D005819:Genetic Markers; D051528:Guanylate Kinases / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D020411:Oligonucleotide Array Sequence Analysis; D020033:Protein Isoforms; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D015854:Up-Regulation
D005819:Genetic Markers; D020033:Protein Isoforms; D025521:Tumor Suppressor Proteins; C496468:DLG2 protein, human; D051528:Guanylate Kinases