Isolation, cloning and sequencing of transferrins from red-eared turtle, African ostrich, and turkey.
Transferrins form an important class of iron-binding proteins widely distributed in the physiological fluids of vertebrates and invertebrates. In vertebrates they are present mostly in serum as serotransferrins. In birds and reptiles transferrins are also found in eggs as ovotransferrins. However, until now only chicken and duck ovotransferrin sequences have been published. This paper presents data on the purification, biochemical analysis, cloning and sequencing of ovotransferrins from red-eared turtle, African ostrich and turkey, revealing their significant homology with other known ovotransferrin sequences. The proteins were purified by size-exclusion and anion-exchange chromatography. Isoelectric points, iron-saturated and iron-free spectra, as well as the mRNA nucleotide sequences of 2,409 nt (ORF: 2,106 nt encoding a 701-amino-acid polypeptide; ), 2,418 nt (ORF 2,118 nt encoding a 705-amino-acid polypeptide; ), and 2,397 nt (ORF: 2,118 nt encoding a 705-amino-acid polypeptide; ) were determined for ostrich (OtrF), red-eared turtle (TtrF), and turkey (MtrF) ovotransferrin, respectively.
Ciuraszkiewicz
Justyna
J
Laboratory of Biochemistry, Institute of Biochemistry and Molecular Biology, Wroclaw University, Tamka 2, 50-137 Wroclaw, Poland.
Olczak
Mariusz
M
Watorek
Wiesław
W
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003207:Conalbumin / Q000235:genetics* / Q000302:isolation & purification*; D018076:DNA, Complementary / Q000302:isolation & purification; D004527:Egg Proteins / Q000302:isolation & purification; D006031:Glycosylation; D007501:Iron / Q000378:metabolism; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013057:Spectrum Analysis; D020310:Struthioniformes / Q000235:genetics*; D014422:Turkeys / Q000235:genetics*; D014426:Turtles / Q000235:genetics*
D018076:DNA, Complementary; D004527:Egg Proteins; D003207:Conalbumin; D007501:Iron