dye (arc) Mutants: insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants.
arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels.
Ruiz
Jimena A
JA
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Fernández
Rubén O
RO
Nikel
Pablo I
PI
Méndez
Beatriz S
BS
Pettinari
M Julia
MJ
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D006885:Hydroxybutyrates / Q000378:metabolism*; D009154:Mutation; D010101:Oxygen Consumption; D010641:Phenotype; D011091:Polyesters / Q000378:metabolism*; D017382:Reactive Oxygen Species; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D000094122:Polyhydroxybutyrates
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D006885:Hydroxybutyrates; D011091:Polyesters; D017382:Reactive Oxygen Species; D012097:Repressor Proteins; C041610:arcA protein, E coli; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates