Increased gene expression of glutathione peroxidase-3 in diabetic mouse heart.
Increased incidence of heart disease is reported in patients with diabetes. To elucidate a molecular profile of expressed genes during the progression of diabetes, a cDNA array was screened in the hearts of mice treated with streptozotocin (200 mg/kg, i.v.). Among the genes investigated, the plasma type glutathione peroxidase, GPX-3, was predominantly up-regulated in diabetic mice compared with control mice. In northern blot analysis, a significant increase in GPX-3 expression was observed as early as 5 d after the induction of hyperglycemia. On day 21, a nearly three-fold induction was demonstrated. Daily administration of insulin (0.2 U/d, s.c.) for 21 d almost completely abolished the increase in GPX-3 mRNA in streptozotocin-treated mice, suggesting that the expression level of the GPX-3 gene was dependent on insulin and serum glucose. Increased GPX-3 may play a significant role in protecting cardiomyocytes from oxidative stress caused by hyperglycemia.
Iwata
Kazumi
K
Department of Pharmacology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Nishinaka
Toru
T
Matsuno
Kuniharu
K
Yabe-Nishimura
Chihiro
C
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015152:Blotting, Northern; D001835:Body Weight / Q000502:physiology; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000096:biosynthesis* / Q000235:genetics*; D006943:Hyperglycemia / Q000201:enzymology; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D007328:Insulin / Q000627:therapeutic use; D008297:Male; D051379:Mice; D009206:Myocardium / Q000201:enzymology*; D020411:Oligonucleotide Array Sequence Analysis; D009929:Organ Size / Q000502:physiology; D015854:Up-Regulation
D001786:Blood Glucose; D018076:DNA, Complementary; D007004:Hypoglycemic Agents; D007328:Insulin; C116888:Gpx3 protein, mouse; D005979:Glutathione Peroxidase