Tick troponin I-like molecule is a potent inhibitor for angiogenesis.
This is the first report identifying the anti-angiogenesis saliva molecule of the ixodid tick. In our previous study, we identified a troponin I-like molecule (HLTnI) of the ixodid hard tick Haemaphysalis longicornis, a vector for various pathogens. To investigate its potential inhibitory effects for angiogenesis, we expressed and purified recombinant HLTnI in Escherichia coli. In a vascular endothelial growth factor (VEGF) competitive angiogenesis assay, the recombinant HLTnI significantly inhibited the capillary formation of human vascular endothelial cells (HUVEC) in vitro. The inhibition was dose-dependent with an IC(50) of 18.95 nM. These results indicated that HLTnI is a potent angiogenesis inhibitor.
Fukumoto
Shinya
S
Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Japan.
Sakaguchi
Takeshi
T
You
Myungio
M
Xuan
Xuenan
X
Fujisaki
Kozo
K
D020533:Angiogenesis Inhibitors / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D026863:Ixodidae / Q000378:metabolism*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D019210:Troponin I / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
D020533:Angiogenesis Inhibitors; D011994:Recombinant Proteins; D012471:Salivary Proteins and Peptides; D019210:Troponin I