title: Regulation of sulfate uptake by excised barley roots in the presence of selenate.
abstract: Active transport of SO(4) (2-) and SeO(4) (2-) has been evaluated during 60-hour contact of barley roots with nutrient solutions containing either (35)SO(4) (2-) or (75)SeO(4) (2-), or both ions, at 0.1 milli-equivalent per liter. In the SO(4) (2-) solution the time course of active transport follows a straight line; if SeO(4) (2-) is also present transport is strongly inhibited after 20 to 30 hours for both ions. The S-Se uptake ratio remains 1.4 during the 60 hours; S-Se ratio shifts from 3.0 to 3.3 in proteins and falls to 0.6 in free amino acids. S-Se discrimination is mainly operating at the level of amino acid incorporation into proteins. The presence of Se-amino acids blocks this incorporation and brings about an accumulation of free amino acids; at the same time carrier activity is inhibited. The addition of methionine or Se-methionine causes a 60 to 80% inhibition of the active transport.
authors.affiliation: Institute of Agricultural Chemistry, University of Padua, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ferrari
authors.forename: F
authors.initials: F
authors.lastname: Renosto