Irisolidone, an isoflavone metabolite, represses JC virus gene expression via inhibition of Sp1 binding in human glial cells.
Progressive multifocal leukoencephalopathy (PML) is a fatal demyelinating disease that results from an oligodendrocyte infection caused by the JC virus. Therefore, inhibiting the expression of JC virus is important for preventing and/or treating PML. This study found that irisolidone, an isoflavone metabolite, significantly inhibited the JC virus expression in primary cultured human astrocytes and glial cell lines. Studies examining the underlying mechanism revealed that a mutation of the Sp1 binding site downstream of the TATA box (Sp1-II) dramatically diminished the inhibitory activity of irisolidone. In addition, an irisolidone treatment repressed Sp1 binding to Sp1-II site, which is important for the basal JC virus promoter activity. The results suggest that the inhibitory effect of irisolidone against the JC virus may be attributed at least in part to the suppression of Sp1 binding to the JC virus promoter region. Therefore, the inhibition of the JC virus expression by irisolidone might provide therapeutic potential for PML caused by the JC virus.
Kim
So-Young
SY
Department of Neuroscience and Medical Research Institute, College of Medicine, Ewha Womans University, Seoul, Republic of Korea.
Kim
Dong-Hyun
DH
Hyun
Jin-Won
JW
Henson
John W
JW
Kim
Hee-Sun
HS
D001483:Base Sequence; D002478:Cells, Cultured; D005419:Flavonoids / Q000494:pharmacology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D007529:Isoflavones / Q000378:metabolism; D007577:JC Virus / Q000187:drug effects* / Q000235:genetics; D007968:Leukoencephalopathy, Progressive Multifocal / Q000821:virology*; D009154:Mutation; D009457:Neuroglia / Q000378:metabolism / Q000821:virology; D027601:Polyomavirus Infections / Q000821:virology*; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D016329:Sp1 Transcription Factor / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016385:TATA Box / Q000235:genetics
D005419:Flavonoids; D007529:Isoflavones; D016329:Sp1 Transcription Factor; C423902:irisolidone