title: A Cyanobacterial Gene Encoding Peptidyl-Prolyl cis-trans Isomerase.
abstract: The rotA gene encoding peptidyl-prolyl cis-trans isomerase has been identified, sequenced, and shown to be transcribed in the cyanobacterium Synechococcus sp. PCC7942. Inactivation of the gene by replacement of a region containing the open reading frame with a gene conferring kanamycin resistance resulted in merodiploids containing both the wild type and the modified genomic region. We were not able to isolate a kanamycin-resistant mutant in which all the genomic wild-type copies were substituted, which suggests that such replacement could have been lethal.
authors.affiliation: Department of Botany, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Hassidim
authors.forename: R
authors.initials: R
authors.lastname: Schwarz
authors.forename: J
authors.initials: J
authors.lastname: Lieman-Hurwitz
authors.forename: E
authors.initials: E
authors.lastname: Marco
authors.forename: M
authors.initials: M
authors.lastname: Ronen-Tarazi
authors.forename: A
authors.initials: A
authors.lastname: Kaplan