Expression of insulin-like growth factor-2 receptors on EL4 lymphoma cells overexpressing growth hormone.
In the present study, we report the upregulation of functional IGF-2Rs in cells overexpressing growth hormone (GH). EL4 lymphoma cells stably transfected with an rGH cDNA overexpression vector (GHo) exhibited an increase in the binding of (125)I-IGF-2 with no change in the binding affinity compared to vector alone controls. An increase in the expression of the insulin-like growth factor-2 receptor (IGF-2R) in cells overexpressing GH was confirmed by Western blot analysis and IGF-2R promoter luciferase assays. EL4 cells produce insulin-like growth factor-2 (IGF-2) as detected by the reverse transcription-polymerase chain reaction (RT-PCR); however, no IGF-2 protein was detected by Western analysis. The increase in the expression of the IGF-2R resulted in greater levels of IGF-2 uptake in GHo cells compared to vector alone controls. The data suggest that one of the consequences of the overexpression of GH is an increase in the expression of the IGF-2R.
Farmer
John T
JT
Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, AL 35294-0005, USA.
Weigent
Douglas A
DA
D000818:Animals; D045744:Cell Line, Tumor; D013006:Growth Hormone / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D008223:Lymphoma; D051379:Mice; D017527:Receptor, IGF Type 2 / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D015854:Up-Regulation
D017527:Receptor, IGF Type 2; D007335:Insulin-Like Growth Factor II; D013006:Growth Hormone