Hydride transfer versus hydrogen radical transfer in thymidylate synthase.
The nature of a H-transfer in the thymidylate synthase catalyzed reaction was investigated by comparison of the wild-type enzyme with the W80M mutant. The nature of the H-transfer was not affected, as indicated by intrinsic isotope effects and their temperature dependence. These findings support a single-step hydride transfer instead of a two-step radical transfer.
Hong
Baoyu
B
Department of Chemistry, University of Iowa, Iowa City, Iowa 52242-1294, USA.
Haddad
Majd
M
Maley
Frank
F
Jensen
Jan H
JH
Kohen
Amnon
A
D006859:Hydrogen / Q000737:chemistry*; D013940:Thymidylate Synthase / Q000737:chemistry*
D006859:Hydrogen; D013940:Thymidylate Synthase