title: Spectroscopic spectral-domain optical coherence microscopy.
abstract: The spectroscopic content within optical coherence tomography (OCT) data can provide a wealth of information. Spectroscopic OCT methods are frequently limited by time-frequency trade-offs that limit high spectral and spatial resolution simultaneously. We present spectroscopic spectral-domain optical coherence microscopy performed with a multimodality microscope. Restricting the spatial extent of the signal by using high-numerical-aperture optics makes high-resolution spectroscopic information accessible, facilitated with spectral-domain detection. Simultaneous acquisition of multiphoton microscopy images is used to validate tissue structure and localization of nuclei within individual cells.
authors.affiliation: Biophotonics Imaging Laboratory, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, Illinois 61801, USA.
authors.forename: Chengyang
authors.initials: C
authors.lastname: Xu
authors.forename: Claudio
authors.initials: C
authors.lastname: Vinegoni
authors.forename: Tyler S
authors.initials: TS
authors.lastname: Ralston
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Wei
authors.initials: W
authors.lastname: Tan
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Boppart
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D051381:Rats; D013057:Spectrum Analysis / Q000379:methods*; D041623:Tomography, Optical Coherence / Q000379:methods*