Analytical, physiologic, and clinical validation of a radioimmunoassay for measurement of procollagen type III amino terminal propeptide in serum and bronchoalveolar lavage fluid obtained from dogs.
OBJECTIVE
To validate a radioimmunoassay for measurement of procollagen type III amino terminal propeptide (PIIINP) concentrations in canine serum and bronchoalveolar lavage fluid (BALF) and investigate the effects of physiologic and pathologic conditions on PIIINP concentrations.


METHODS
Sera from healthy adult (n = 70) and growing dogs (20) and dogs with chronic renal failure (CRF; 10), cardiomyopathy (CMP; 12), or degenerative valve disease (DVD; 26); and sera and BALF from dogs with chronic bronchopneumopathy (CBP; 15) and healthy control dogs (10 growing and 9 adult dogs).


METHODS
A radioimmunoassay was validated, and a reference range for serum PIIINP (S-PIIINP) concentration was established. Effects of growth, age, sex, weight, CRF, and heart failure on S-PIIINP concentration were analyzed. In CBP-affected dogs, S-PIIINP and BALF-PIIINP concentrations were evaluated.


RESULTS
The radioimmunoassay had good sensitivity, linearity, precision, and reproducibility and reasonable accuracy for measurement of S-PIIINP and BALF-PIIINP concentrations. The S-PIIINP concentration reference range in adult dogs was 8.86 to 11.48 mug/L. Serum PIIINP concentration correlated with weight and age. Growing dogs had significantly higher S-PIIINP concentrations than adults, but concentrations in CRF-, CMP-, DVD-, or CBP-affected dogs were not significantly different from control values. Mean BALF-PIIINP concentration was significantly higher in CBP-affected dogs than in healthy adults.


CONCLUSIONS
In dogs, renal or cardiac disease or CBP did not significantly affect S-PIIINP concentration; dogs with CBP had high BALF-PIIINP concentrations. Data suggest that the use of PIIINP as a marker of pathologic fibrosis might be limited in growing dogs.
Schuller
Simone
S
Department of Small Animal Clinical Sciences, University of Liège, 4000 Liège, Belgium.
Valentin
Sophie
S
Remy
Benoit
B
Jespers
Pascale
P
Foulon
Suzanne
S
Van Israël
Nicole
N
Clercx
Cécile
C
McEntee
Kathleen
K
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D001996:Bronchopneumonia / Q000097:blood / Q000662:veterinary; D009202:Cardiomyopathies / Q000097:blood / Q000662:veterinary; D002908:Chronic Disease; D004283:Dog Diseases / Q000097:blood* / Q000378:metabolism*; D004285:Dogs; D005260:Female; D006349:Heart Valve Diseases / Q000097:blood / Q000662:veterinary; D007676:Kidney Failure, Chronic / Q000097:blood / Q000662:veterinary; D008297:Male; D010446:Peptide Fragments / Q000032:analysis* / Q000097:blood; D011347:Procollagen / Q000032:analysis* / Q000097:blood; D011863:Radioimmunoassay / Q000662:veterinary*; D015203:Reproducibility of Results
D010446:Peptide Fragments; D011347:Procollagen; C041876:procollagen Type III-N-terminal peptide