title: The cytotoxic saponin from heat-processed Achyranthes fauriei roots.
abstract: An extract of heat-processed Achyranthes fauriei roots showed more potent cytotoxic activity against human hepatoma SK-Hep-1 cells than that of the crude plant. Employing a bioassay-linked HPLC-ELSD (high performance liquid chromatography-evaporative light scattering detector) method, followed by silica gel column chromatography, a cytotoxic triterpenoid saponin, chikusetsusaponin IVa was isolated. It is contributive to the increased cytotoxic activity of the heat-processed. The amount of chikusetsusaponin IVa from the roots of Achyranthes fauriei was significantly increased after heat-processing.
authors.affiliation: Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Seoul.
authors.forename: Hye Hyun
authors.initials: HH
authors.lastname: Yoo
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kwon
authors.forename: Jeong Hill
authors.initials: JH
authors.lastname: Park
mesh_terms: D029044:Achyranthes / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D006358:Hot Temperature; D006801:Humans; D008027:Light; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000494:pharmacology*; D012542:Scattering, Radiation; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue