Wide variety of locations for rodent MATE1, a transporter protein that mediates the final excretion step for toxic organic cations.
MATE1 was the first mammalian example of the multidrug and toxin extrusion (MATE) protein family to be identified. Human MATE1 (hMATE1) is predominantly expressed and localized to the luminal membranes of the urinary tubules and bile canaliculi and mediates H(+)-coupled electroneutral excretion of toxic organic cations (OCs) into urine and bile (Otsuka M, Matsumoto T, Morimoto R, Arioka S, Omote H, and Moriyama Y. Proc Natl Acad Sci USA 102: 17923-17928, 2005). mMATE1, a mouse MATE ortholog, is also predominantly expressed in kidney and liver, although its transport properties are not yet characterized. In the present study, we investigated the transport properties and localization of mMATE1. Upon expression of this protein in HEK-293 cells, mMATE1 mediated electroneutral H(+)/tetraethylammonium exchange and showed a substrate specificity similar to that of hMATE1. Immunological techniques with specific antibodies against mMATE1 combined with RT-PCR revealed that mMATE1 is also expressed in various cells, including brain glia-like cells and capillaries, pancreatic duct cells, urinary bladder epithelium, adrenal gland cortex, alpha cells of the islets of Langerhans, Leydig cells, and vitamin A-storing Ito cells. These results indicate that mMATE1 is a polyspecific H(+)/OC exchanger. The unexpectedly wide distribution of mMATE1 suggests involvement of this transporter protein in diverse biological functions other than excretion of OCs from the body.
Hiasa
Miki
M
Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Matsumoto
Takuya
T
Komatsu
Toshinori
T
Moriyama
Yoshinori
Y
D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D006801:Humans; D007158:Immunologic Techniques; D051379:Mice; D008969:Molecular Sequence Data; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D011522:Protons; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013379:Substrate Specificity; D019789:Tetraethylammonium / Q000378:metabolism; D014018:Tissue Distribution
C514424:MATE1 protein, mouse; D027701:Organic Cation Transport Proteins; D011522:Protons; D019789:Tetraethylammonium