[Hydrogen sulphide water balneum effect on erythrocyte catalase activity in patients with rheumatoid arthritis--in vitro study].
The aim of the study was to investigate, in vitro, hydrogen sulphide water (HSW) balneum effect on erythrocyte catalase activity in patients with rheumatoid arthritis. Erythrocytes from twenty nine consecutive patients with rheumatoid arthritis (11 men, 18 women) aged 54 years were obtained. The control group comprised of 30 healthy subjects with a mean age of about 40 years. Patients with rheumatoid arthritis were subdivided into two groups twice: with active disease (18 patients) and in remission (11 patients), and secondly into patients receiving (21 subjects) and not receiving (8 subjects) non-steroidal anti-inflammatory drugs. For erythrocyte catalase activity evaluation, method of Beers and Sizer was used. Catalase activity was assessed after 5, 10, 15, and 20 minutes erythrocytes incubation with HSW. The mean baseline erythrocyte catalase activity (to) was in rheumatoid arthritis patients of about 7.79 +/- 1.39 U/gHb and was significantly higher than in the control group: 6.96 +/- 2.68 U/gHb (p < 0.05). After 5 minutes incubation with HSW (t5) erythrocyte catalase activity increased, in rheumatoid arthritis patients to 8.21 +/- 1.77 U/gHb, after 10 minutes (t10) was 8.14 +/- 2.25 U/gHb, in control group: 7.58 +/- 2.50 U/gHb and 7.68 +/- 3.22 U/gHb, respectively. However the difference was not statistically significant. After 20 minutes of incubation (t20) erythrocyte catalase activity was the highest in the patients with active rheumatoid arthritis (8.33 +/- 1.96 U/gHb) and differed significantly from the patients in remission (6.69 +/- 1.27 U/gHb) and from patients not receiving non-steroidal anti-inflammatory drugs (6.04 +/- 1.08 U/gHb). In rheumatoid arthritis patients erythrocyte catalase activity was higher when compared with control group and increased during incubation with HSW. It seems HSW balneum produce an antioxidant effect on erythrocyte status in patients with rheumatoid arthritis.
Wozakowska-Kapłon
Beata
B
Zakładu Profilaktyki Chorób Układu Krazenia Zdrowiu Akademii Swietokrzyskiej w Kielcach.
Grabski
Marek
M
Kedziora
Józef
J
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000628:therapy*; D001452:Balneology / Q000379:methods*; D002374:Catalase / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D012720:Severity of Illness Index
D002374:Catalase; D006862:Hydrogen Sulfide