title: Biosynthesis of cytidine diphosphate diglyceride by enzyme preparations from cauliflower.
abstract: The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activity was greater when fluoride was included in the reaction mixture. The enzyme has a pH optimum of 5.6, an optimal Mn(2+) concentration of 1mm and an optimal Mg(2+) concentration of 30 mm. At the optimal concentration, Mn(2+) was 4 times better than Mg(2+). The utilization of biosynthesized cytidine diphosphate diglyceride can be enhanced by addition of inositol, thereby causing the formation of phosphatidyl inositol.
authors.affiliation: Department of Biochemistry and Statewide Air Pollution Research Center, University of California, Riverside, California 92502.
authors.forename: S
authors.initials: S
authors.lastname: Sumida
authors.forename: J B
authors.initials: JB
authors.lastname: Mudd