Study on pharmacokinetics and tissue distribution of norvancomycin in rats by CE with electrochemical detection.
In this paper, we developed a sensitive and simple method to study the pharmacokinetics and tissue distribution of norvancomycin (NVCM) in experimental animals by using CE with electrochemical detection. Pharmacokinetics investigation was performed by the collection of blood samples at timed intervals following administration of NVCM. Pharmacokinetic parameters were calculated by the 3P87 pharmacokinetic program. The elimination half-life of NVCM was 42.4742 min with a clearance rate of 0.0233 mL x kg(-1) x min(-1). Additionally, drug distribution was studied by measuring the NVCM levels in kidney, lung, stomach, intestine, spleen, heart, liver, and cerebrum. Electrophoresis conditions such as buffer solution, working potential, separation voltage, and sampling time were also discussed. The linear range was from 0.8 to 540 microg/mL with a correlation coefficient of 0.9991. The detection limit was 0.3 microg/mL. This method was for the first time applied to study the pharmacokinetics and tissue distributions of NVCM in experimental animals.
Zhou
Tianshu
T
Department of Environmental Science, East China Normal University, Shanghai, P. R. China. tszhou@des.ecnu.edu.cn
Wu
Fang
F
Shi
Guoyue
G
Wang
Aifang
A
Hu
Qin
Q
Wang
Xiaochun
X
Fang
Yuzhi
Y
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006207:Half-Life; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D014018:Tissue Distribution; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics
D000900:Anti-Bacterial Agents; D012996:Solutions; D012710:Serum Albumin, Bovine; D014640:Vancomycin; C511195:norvancomycin