[Effects of Shuanggu Yitong needling method on proliferation and differentiation of nerve stem cells in the Parkinson's disease model rat].
OBJECTIVE
To explore the mechanism of acupuncture in treatment of Parkinson's disease.


METHODS
Fifty Wistar rats were randomly divided into a normal control group, a sham-operation control group, a model group, a Fengfu-Taichong group and a Shuanggu Yitong group. The hemilateral rotation Parkinson's disease model was prepared by micro-injection of 6-hydroxyl-dopamine into the right striatum. Effects of acupuncture at "Fengfu" (GV 16), "Taichong" (LR 3) hy Shoanggu Yitong needling method on proliferation and differentiation of nerve stem cells in the Parkinson's disease model rat were compared. Proliferation and numher changes of the nerve stem cells in the suhstantia nigra and the striatum were investigated with immunohistochemical method, and the number of nerve stem cells transforming neurons were observed with immunohistochemical double-labeling method.


RESULTS
In the two acupuncture groups, the proliferative cells in the substantia nigra and thestriatum of the destroyed side increased significantly (P < 0.01), and the number of nerve stem cells and the number of transformed neurons in the destroyed side of the substantia nigra and the striatum increased significantly only in the Shuanggu Yitong group (P < 0.01).


CONCLUSIONS
Shuanggu Yitong needling method can induce proliferation and differentiation of nerve stem cells in the substantia nigra and the striatum regions in the Parkinson's disease model rat.
Wang
Yan-chun
YC
Department of Acup-Moxibustion, Orthopedics and Traumatology, Wuhan 430061, China. wangyanchunonly@yahoo.com.cn
Ma
Jun
J
Wang
Hua
H
D015670:Acupuncture Therapy / Q000379:methods*; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D003342:Corpus Striatum / Q000473:pathology; D008297:Male; D009474:Neurons / Q000166:cytology*; D010300:Parkinson Disease / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology*; D013378:Substantia Nigra / Q000473:pathology
D001973:Bromodeoxyuridine