No effect of recombinase-mediated DNA transfer on production efficiency of transgenic rats.
It was reported that recombinase-A protein (RecA)-coated exogenous DNA was more likely to be integrated into mouse, goat and pig genomes. The objective of this study was to investigate whether integration of exogenous DNA into the rat genome is improved by the recombinase-mediated DNA transfer. Pronuclear microinjection of RecA-coated EGFP or OAMB DNA resulted in a production efficiency of transgenic rats of 1.4-2.9%, comparable with 0.9-2.6% when non-coated control DNA was used. Intracytoplasmic injection of the sperm heads exposed to RecA-coated EGFP DNA did not produce any transgenic rats (0 vs. 0-2.8% in control groups). Thus, the recombinase-mediated DNA transfer contributed very little to the production of transgenic rats by means of pronuclear microinjection and intracytoplasmic sperm injection.
Hirabayashi
Masumi
M
National Institute for Physiological Sciences, Okazaki, Aichi, Japan.
Kato
Megumi
M
Kaneko
Ryosuke
R
Hirabayashi
Takahiro
T
Morita
Mitsuhiro
M
Hochi
Shinichi
S
D000818:Animals; D030801:Animals, Genetically Modified; D004247:DNA / Q000008:administration & dosage*; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D051381:Rats; D011938:Rec A Recombinases / Q000494:pharmacology*
D004247:DNA; D011938:Rec A Recombinases