Improvement of the acridine orange-protein-surfactant system for protein estimation based on aromatic ring stacking effect of sodium dodecyl benzene sulphonate.
The fluorescence of acridine orange (AO) is greatly quenched by the anionic surfactant sodium dodecyl benzene sulphonate (SDBS), but when protein is added into the AO-SDBS system, the fluorescence intensity of the latter is enhanced again. Based on this, a new fluorimetric method of determination of protein was developed. Under optimum conditions, the enhanced intensity of fluorescence is in proportion to the concentration of protein, such as bovine serum albumin (BSA), human serum albumin (HSA) and egg albumin (EA), over a wide range with detection limits at the 10(-9) g/mL level. This method has been satisfactorily used for the determination of protein in samples. We compared results using 280 nm and 490 nm excitation wavelengths and the mechanism of the assay.
Wang
Fei
F
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
Yang
Jinghe
J
Wu
Xia
X
Wang
Xiaobo
X
Guo
Changying
C
Jia
Zhen
Z
D000165:Acridine Orange / Q000737:chemistry*; D000818:Animals; D001557:Benzenesulfonates / Q000737:chemistry*; D002021:Buffers; D006801:Humans; D006841:Hydrocarbons, Aromatic / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010047:Ovalbumin / Q000032:analysis / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000032:analysis / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*
D001557:Benzenesulfonates; D002021:Buffers; D006841:Hydrocarbons, Aromatic; D011506:Proteins; D012709:Serum Albumin; D013501:Surface-Active Agents; C001114:dodecylbenzenesulfonic acid; D010047:Ovalbumin; D000165:Acridine Orange