Early development of chick embryo respiratory nervous system: an immunohistochemical study.
The extrinsic and intrinsic respiratory nervous systems receive specific contributions from the vagal and sympathetic components. Using specific markers for vagal and sympathetic structures, we studied the distribution patterns of immunoreactivity to galanin (GAL), pituitary adenylate cyclase-activating polypeptide-27 (PACAP) and the tachykinin substance P in extrinsic and intrinsic nerve of chick embryo respiratory system, during development from the very early age to hatching. All peptides studied appeared in the intrinsic and extrinsic nervous systems early. We found substance P in both the vagal and sympathetic systems, PACAP in vagal components alone and GAL mainly in the sympathetic system. The intrinsic nervous system showed high immunoreactivity for all peptides studied. These data accord with the well known early trophic functions that peptides have on the development of nervous networks and modulatory activity on the intrinsic nervous system. The GAL again proves to be the main peptide in chick embryo sympathetic respiratory system.
Vaccaro
R
R
Department of Human Anatomy, University La Sapienza, Via Borelli 50, 00161, Rome, Italy.
Parisi Salvi
E
E
Renda
T
T
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D019004:Galanin / Q000378:metabolism; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology / Q000294:innervation*; D009479:Neuropeptides / Q000378:metabolism*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000196:embryology* / Q000378:metabolism; D014630:Vagus Nerve / Q000196:embryology* / Q000378:metabolism
D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D013373:Substance P; D019004:Galanin