The importance of occupancy rather than affinity of CaV(beta) subunits for the calcium channel I-II linker in relation to calcium channel function.
The Ca(V)beta subunits of voltage-gated calcium channels regulate the trafficking and biophysical properties of these channels. We have taken advantage of mutations in the tyrosine residue within the alpha interaction domain (AID) in the I-II linker of Ca(V)2.2 which reduce, but do not abolish, the binding of beta1b to the AID of Ca(V)2.2. We have found that the mutation Y388S decreased the affinity of Ca(V)beta1b binding to the Ca(V)2.2 I-II linker from 14 to 329 nm. However, the Y388S mutation had no effect on current density and cell surface expression of Ca(V)2.2/alpha2delta-2/beta1b channels expressed in human embryonic kidney tsA-201 cells, when equivalent proportions of cDNA were used. Furthermore, despite the 24-fold reduced affinity of Ca(V)beta1b for the Y388S I-II linker of Ca(V)2.2, all the key features of modulation as well as trafficking by Ca(V)beta subunits remained intact. This is in contrast to the much more marked effect of the W391A mutation, which abolished interaction with the Ca(V)2.2 I-II linker, and very markedly affected the trafficking of the channels. However, using the Xenopus oocyte expression system, where expression levels can be accurately titrated, when Ca(V)beta1b cDNA was diluted 50-fold, all evidence of interaction with Ca(V)2.2 Y388S was lost, although wild-type Ca(V)2.2 was still normally modulated by the reduced concentration of beta1b. These results indicate that high affinity interaction with the alpha1 subunit is not necessary for any of the modulatory effects of Ca(V)beta subunits, but occupancy of the interaction site is important, and this will occur, despite the reduced affinity, if the Ca(V)beta subunit is present in sufficient excess.
Butcher
Adrian J
AJ
Laboratory of Cellular and Molecular Neuroscience, Department of Pharmacology, Andrew Huxley Building, University College London, UK.
Leroy
Jérôme
J
Richards
Mark W
MW
Pratt
Wendy S
WS
Dolphin
Annette C
AC
D000595:Amino Acid Sequence; D000818:Animals; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D020864:Calcium Channels, N-Type / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005260:Female; D019204:GTP-Binding Proteins / Q000502:physiology; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009865:Oocytes; D011485:Protein Binding; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D014982:Xenopus laevis
C500545:CACNA1B protein, human; D015220:Calcium Channels; D020864:Calcium Channels, N-Type; D018076:DNA, Complementary; D019204:GTP-Binding Proteins