Promoter analysis of interleukin 19.
Interleukin (IL)-19 belongs to the IL-10 family which includes IL-19, IL-20, IL-22, AK155, and MDA-7. IL-10 is a potent immunomodulatory cytokine with implications for pathogenesis in various autoimmune diseases. Polymorphism of the IL-10 promoter region correlates with disease outcome. To understand the gene regulation of IL-19, we analyzed the IL-19 promoter region. A regulatory region (PE), 148bp upstream of exon 1 of IL-19 and linked to a luciferase reporter gene, supported luciferase activity 13 times greater than that supported by a negative promoterless control. An electrophoretic mobility shift assay (EMSA) showed specific binding sites for the transcription factors of the oligonucleotides PE1 (-148 to -98) derived from PE. We identified the sequence TGTGGT (-142 to -138) on PE1 as the binding site for the transcription factor AML1, and crucial for the promoter activity of IL-19 because substituting 1bp in the PE region (-139G-->T) abolished IL-19 promoter activity.
Chen
Po-Jen
PJ
Chi-Mei Medical Center, Tainan, Taiwan.
Wei
Chi-Chen
CC
Wang
Chihuei
C
Chen
Feng-Wei
FW
Hsu
Yu-Hsiang
YH
Chang
Ming-Shi
MS
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics*; D004285:Dogs; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D007668:Kidney / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D050676:Core Binding Factor Alpha 2 Subunit; C421250:IL19 protein, human; D007378:Interleukins; C493728:RUNX1 protein, human; D014157:Transcription Factors