Controlled microfluidic encapsulation of cells, proteins, and microbeads in lipid vesicles.
Cells have been encapsulated inside lipid vesicles by using a new microfluidic lipid vesicle formulation technique. Lipid vesicles are formulated within minutes without using toxic lipid solvents. The encapsulation efficiency inside the vesicles is controlled by the microfluidic flows. Green fluorescent proteins (GFP), carcinoma cells, and bead encapsulated vesicles have mean diameters of 27.2 mum, 62.4 mum, and 55.9 mum, respectively. The variations of vesicle sizes are approximately 20% for the GFP and cell encapsulated vesicles and approximately 10% for the bead encapsulated vesicles.
Tan
Yung-Chieh
YC
University of California Irvine, Department of Biomedical Engineering, Irvine, California 92697, USA. ytan@uci.edu
Hettiarachchi
Kanaka
K
Siu
Maria
M
Pan
Yen-Ru
YR
Lee
Abraham Phillip
AP
D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D044085:Microfluidics; D008863:Microspheres; D011506:Proteins / Q000737:chemistry*
D008055:Lipids; D011506:Proteins