Differential display profiling of the Nicotiana response to LPS reveals elements of plant basal resistance.
The identification of cDNAs, representing up-regulated genes induced by lipopolysaccharides from Burkholderia cepacia, was achieved by differential display of mRNAs isolated from tobacco cells. In addition to up-regulation of superoxide dismutase, involved in the production of the signalling and defense molecule, hydrogen peroxide; differentially expressed cDNAs, indicative of the operation of an innate immune recognition system and expression of basal resistance, were identified. These include homologs to a receptor-like protein kinase; a binding protein for the type III secreted effector protein, harpin; a virus resistance N gene; an endogenous pararetrovirus and the Pto kinase. The altered gene expression may be responsible for activation of surveillance mechanisms and enhancement of the non-self recognition capacity. The putative roles of these transcripts in LPS-induced responses are discussed in relation to emerging concepts of innate immunity.
Sanabria
Natasha M
NM
Department of Biochemistry, University of Johannesburg, Kingsway Campus, P.O. Box 524, Auckland Park 2006, South Africa.
Dubery
Ian A
IA
D002478:Cells, Cultured; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D007113:Immunity, Innate / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D010935:Plant Diseases / Q000139:chemically induced; D010940:Plant Proteins / Q000378:metabolism*; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism*
D008070:Lipopolysaccharides; D010940:Plant Proteins