title: NMR studies of BPTI aggregation by using paramagnetic relaxation reagents.
abstract: Paramagnetic probes, whose approach to proteins can be monitored by nuclear magnetic resonance (NMR) studies, have been found of primary relevance for investigating protein surfaces accessibility. Here, paramagnetic probes are also suggested for a systematic investigation on protein aggregation. Bovine pancreatic trypsin inhibitor (BPTI) was used as a model system for aggregation by analyzing its interaction with TEMPOL and Gd(III)DTPA-BMA. Some of the measured paramagnetic relaxation rates of BPTI protons exhibited a reverse dependence on protein concentration, which can be attributed to the formation of transient BPTI aggregates.
authors.affiliation: Biomolecular Structure Research Center and Dipartimento di Biologia Molecolare, Università di Siena, Via A. Fiorentina, I-53100 Siena, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Bernini
authors.forename: Ottavia
authors.initials: O
authors.lastname: Spiga
authors.forename: Arianna
authors.initials: A
authors.lastname: Ciutti
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Venditti
authors.forename: Filippo
authors.initials: F
authors.lastname: Prischi
authors.forename: Mariangela
authors.initials: M
authors.lastname: Governatori
authors.forename: Luisa
authors.initials: L
authors.lastname: Bracci
authors.forename: Barbara
authors.initials: B
authors.lastname: Lelli
authors.forename: Silvia
authors.initials: S
authors.lastname: Pileri
authors.forename: Mauro
authors.initials: M
authors.lastname: Botta
authors.forename: Alessandro
authors.initials: A
authors.lastname: Barge
authors.forename: Franco
authors.initials: F
authors.lastname: Laschi
authors.forename: Neri
authors.initials: N
authors.lastname: Niccolai
mesh_terms: D000818:Animals; D007611:Aprotinin / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D019786:Gadolinium DTPA; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D025941:Protein Interaction Mapping; D013113:Spin Labels; D013499:Surface Properties
chemical_list: D003497:Cyclic N-Oxides; D013113:Spin Labels; D007611:Aprotinin; D019786:Gadolinium DTPA; C001803:tempol