title: [Inhibitory effect of dendritic cell vaccine on the growth of implanted tongue squamous cell carcinoma tumors in nude mice].
abstract: OBJECTIVE
  To investigate the inhibitory effect of dendritic cells (DCs) on the growth of the implanted tongue squamous cell carcinoma (SCC) tumors in nude mice.
  
  
  METHODS
  Being induced from human peripheral blood monocytes (PBMCs) with rhGM-CSF, rhIL-4 and rhTNF-alpha, DCs were pulsed by Tca8113 cells lysates. Those DCs and T lymphocytes were co-cultured to induce specific cytotoxic T lymphocytes (CTLs). Immunotherapy was then performed after those DCs and CTLs were implanted into the tumor-bearing nude mice.
  
  
  RESULTS
  DCs were induced from PBMCs with multiple cytokines. Compared with the control groups, the growth of tumors was significantly inhibited in the group that had been implanted with DCs and CTLs, and tumor doubling time also increased markedly (P < 0.05).
  
  
  CONCLUSIONS
  DCs function normally after being induced from human PBMCs with multiple cytokines; DCs pulsed with tumor cell lysates and CTLs co-cultured with those DCs have a significant anti-tumor immune activity in nude mice.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Stomatological Hospital Affiliated to Medical college of Nanjing University, Nanjing 210008, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Qin-gang
authors.initials: QG
authors.lastname: Hu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Wang
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000628:therapy*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D007167:Immunotherapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000628:therapy; D014062:Tongue Neoplasms / Q000628:therapy*
chemical_list: D019496:Cancer Vaccines