title: The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii.
abstract: ISAba1 was found in all widespread clones of Acinetobacter baumannii in the United Kingdom. All isolates studied had a blaOXA-51-like carbapenemase gene; some also had blaOXA-23-like and/or blaOXA-58-like. Among isolates with blaOXA-51-like as sole carbapenemase gene, only those with ISAba1 adjacent to blaOXA-51-like were carbapenem resistant. Minor differences in blaOXA-51-like sequence were observed in resistant and susceptible isolates. Isolates with blaOXA-23-like in addition were consistently resistant to carbapenems; in all of these ISAba1 lay upstream of blaOXA-23-like, but was not associated with blaOXA-51-like. These results suggest that ISAba1 is providing the promoter for blaOXA-51-like and, probably, for blaOXA-23-like.
authors.affiliation: Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, London, UK. jane.turton@hpa.org.uk
authors.forename: Jane F
authors.initials: JF
authors.lastname: Turton
authors.forename: M Elaina
authors.initials: ME
authors.lastname: Ward
authors.forename: Neil
authors.initials: N
authors.lastname: Woodford
authors.forename: Mary E
authors.initials: ME
authors.lastname: Kaufmann
authors.forename: Rachel
authors.initials: R
authors.lastname: Pike
authors.forename: David M
authors.initials: DM
authors.lastname: Livermore
authors.forename: Tyrone L
authors.initials: TL
authors.lastname: Pitt
mesh_terms: D040981:Acinetobacter baumannii / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004251:DNA Transposable Elements / Q000502:physiology*; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases; C063912:carbapenemase