title: Bioactivity profiling using HPLC/microtiter-plate analysis: application to a New Zealand marine alga-derived fungus, Gliocladium sp.
abstract: Using HPLC/microtiter-plate-based generation of activity profiles the extract of a marine alga-derived fungus, identified as Gliocladium sp., was shown to contain the known strongly cytotoxic metabolite 4-keto-clonostachydiol (1) and also clonostachydiol (2) as well as gliotide (3), a new cyclodepsipeptide containing several D-amino acids. The absolute configuration of 1 was elucidated by reduction to 2, and two further oxidized derivatives of clonostachydiol (5, 6) were prepared and evaluated for biological activity.
authors.affiliation: Department of Chemistry and School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Lang
authors.forename: Maya I
authors.initials: MI
authors.lastname: Mitova
authors.forename: Gill
authors.initials: G
authors.lastname: Ellis
authors.forename: Sonia
authors.initials: S
authors.lastname: van der Sar
authors.forename: Richard K
authors.initials: RK
authors.lastname: Phipps
authors.forename: John W
authors.initials: JW
authors.lastname: Blunt
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Cummings
authors.forename: Anthony L J
authors.initials: AL
authors.lastname: Cole
authors.forename: Murray H G
authors.initials: MH
authors.lastname: Munro
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D056890:Eukaryota; D020462:Gliocladium / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007941:Leukemia P388; D008386:Marine Biology; D051379:Mice; D015394:Molecular Structure; D009520:New Zealand; D010084:Oxidation-Reduction
chemical_list: C510703:4-keto-clonostachydiol; D000970:Antineoplastic Agents; D047630:Depsipeptides; D007783:Lactones; C510704:gliotide; C080565:clonostachydiol