title: Increased oxidative stress is associated with decreased circulating levels of adiponectin in Japanese metabolically obese, normal-weight men with normal glucose tolerance.
abstract: To investigate the relationship between oxidative stress and circulating levels of adiponectin in Japanese metabolically obese, normal-weight [MONW; BMI<25 and visceral fat area; VFA > or =100 cm2 by abdominal computed tomography (CT) scanning] men with normal glucose tolerance (NGT), we measured the plasma levels of free 8-epi-prostaglandin F2alpha (8-epi-PGF2alpha) and adiponectin in 28 MONW and 23 normal men. The plasma levels of free 8-epi-PGF2alpha were measured using a commercially available enzyme immunoassay (EIA) kit (Cayman Chemical, Ann Arbor, MI). The plasma levels of adiponectin were measured using a radioimmunoassay kit (LINCO Research, St. Charles, MO). Plasma levels of 8-epi-PGF2alpha in MONW subjects (30.4+/-4.0 pg/ml; P<0.01) were significantly increased compared to controls (8.1+/-1.3 pg/ml). The plasma levels of adiponectin were significantly decreased in MONW subjects (8.6+/-0.9 microg/ml; P<0.01) as compared to normal subjects (11.6+/-0.6 microg/ml). The plasma levels of 8-epi-PGF2alpha and adiponectin were significantly correlated in MONW (r=-0.617, P<0.01) and in all (MONW+normal) (r=-0.620, P<0.01) subjects. The plasma levels of 8-epi-PGF2alpha and adiponectin were significantly correlated after adjustment for VFA in MONW subjects (F=11.042, P<0.01). The present study showed that systemic increase in oxidative stress correlates with decreased circulating levels of adiponectin in Japanese MONW men with NGT. Although correlation does not prove causation, this observation suggests that increased oxidative stress may decrease the production of adiponectin in Japanese MONW men with NGT.
authors.affiliation: Department of Internal Medicine, Division of Diabetology and Endocrinology, Mie University School of Medicine, Tsu, Mie, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Katsuki
authors.forename: Mina
authors.initials: M
authors.lastname: Suematsu
authors.forename: Esteban C
authors.initials: EC
authors.lastname: Gabazza
authors.forename: Shuichi
authors.initials: S
authors.lastname: Murashima
authors.forename: Kaname
authors.initials: K
authors.lastname: Nakatani
authors.forename: Kenji
authors.initials: K
authors.lastname: Togashi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yano
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Adachi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Sumida
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000502:physiology*; D015237:Dinoprost / Q000097:blood; D005951:Glucose Tolerance Test / Q000379:methods; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism; D007564:Japan; D008055:Lipids / Q000097:blood; D008297:Male; D009765:Obesity / Q000097:blood / Q000378:metabolism / Q000503:physiopathology*; D018384:Oxidative Stress / Q000502:physiology*; D011863:Radioimmunoassay
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D008055:Lipids; D015237:Dinoprost