The use of combination subtyping in the forensic evaluation of a three-year-old girl with gonorrhea.
The use of pulsed field gel electrophoresis and neisserial lipoprotein gene sequencing for subtyping Neisseria gonorrhoeae has not been reported in the evaluation of sexually abused children. We report the application and implications of combining pulsed field gel electrophoresis and lipoprotein subtyping in the evaluation of a 3-year-old girl with N. gonorrhoeae infection.
DeMattia
Amy
A
Department of General Pediatrics, Mount Sinai School of Medicine, New York, NY 10029, USA. Amy.demattia@msnyuhealth.org
Kornblum
John S
JS
Hoffman-Rosenfeld
Jamie
J
Trees
David L
DL
Tumpey
Abbigail J
AJ
Laraque
Danielle
D
D000293:Adolescent; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D002650:Child Abuse, Sexual / Q000175:diagnosis*; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D005838:Genotype; D006069:Gonorrhea / Q000382:microbiology*; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
D001425:Bacterial Outer Membrane Proteins; C079135:Lip antigen