title: Response of human peritoneal mesothelial cells to inflammatory injury is regulated by interleukin-1beta and tumor necrosis factor-alpha.
abstract: Peritoneal injury is often associated with alterations of the mesothelium, resulting in peritoneal healing and adhesion formation. We analyzed the effects of pro-inflammatory cytokines on cell morphology and proliferation of human peritoneal mesothelial cells (HPMC). After 48 hours, HPMC formed a confluent layer with cell volumes of 2,662+/-111 fL. Treatment of HPMC with interleukin-1beta and tumor necrosis factor-alpha (TNF-alpha) induced mesothelial disintegration and alterations in mesothelial cell morphology, which were associated with an interleukin-1beta-triggered increase in cell volume (3,028+/-118 fL; p<0.05) and exfoliation of cells into the supernatants of cell cultures (p<0.05). Whereas TNF-alpha arrested HPMC in the G0/G1 phase (p<0.05), interleukin-1beta caused an increase of cells into the S phase of the cell cycle. In addition, interleukin-1beta and interferon-gamma exerted a proliferative effect on HPMC. These changes were independent from mesothelial Na+/H+ antiporter-1 expression. Our data indicate that the response of HPMC to inflammatory injury is regulated by interleukin-1beta and TNF-alpha reflecting their putative role in peritoneal wound healing and adhesion formation.
authors.affiliation: Institute of Pathology, University of Basel, Basel, Switzerland. sstadlmann@uhbs.ch
authors.forename: Sylvia
authors.initials: S
authors.lastname: Stadlmann
authors.forename: Juergen
authors.initials: J
authors.lastname: Pollheimer
authors.forename: Kathrin
authors.initials: K
authors.lastname: Renner
authors.forename: Alain G
authors.initials: AG
authors.lastname: Zeimet
authors.forename: Felix A
authors.initials: FA
authors.lastname: Offner
authors.forename: Albert
authors.initials: A
authors.lastname: Amberger
mesh_terms: D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects* / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D016023:Integrins / Q000187:drug effects; D007375:Interleukin-1 / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D010537:Peritoneum / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D015815:Cell Adhesion Molecules; D016023:Integrins; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha