title: Investigation of superharmonic sound propagation and imaging in biological tissues in vitro.
abstract: This article presents both theoretical and experimental studies on the superharmonic generation and its imaging in biological tissues. A superharmonic component is defined as a summation of the third-, fourth-, and fifth-order harmonics. A superharmonic signal is produced using an 8-mm-diam, 2.5-MHz planar piston source that is excited by eight-cycle, 2.5-MHz tone bursts. Axial and lateral field distributions of the superharmonic component and the second harmonic are first calculated based on the nonlinear KZK model and then compared with those experimentally determined at two different source pressures of 0.5 and 1 MPa. Results indicate that the amplitude of the superharmonic component can exceed that of the second harmonic, depending on the axial distance and the fundamental pressure amplitude. Also, the 3-dB beamwidth of the superharmonic component is about 23% narrower than that of the second harmonic. Additional experiments are performed in vitro using liver and fatty tissues in transmission mode and produced two-dimensional images using the fundamental, the second harmonic, and the superharmonic signals. Although the clinical applicability of this work still needs to be assessed, these results indicate that the superharmonic image quality is better than that of the other two images.
authors.affiliation: State Key Lab of Modern Acoustics, Institute of Acoustics, Nanjing University, Nanjing 210093, China.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Ma
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiufen
authors.initials: X
authors.lastname: Gong
authors.forename: Yong
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000162:Acoustics; D000273:Adipose Tissue / Q000033:anatomy & histology*; D000818:Animals; D003198:Computer Simulation; D003287:Contrast Media; D020342:Finite Element Analysis; D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008099:Liver / Q000033:anatomy & histology*; D045423:Microbubbles; D017711:Nonlinear Dynamics; D012680:Sensitivity and Specificity; D013552:Swine; D014159:Transducers; D014463:Ultrasonography / Q000379:methods*
chemical_list: D003287:Contrast Media