Effect of Growth Temperature on the Lipid Composition of Cyanidium caldarium: I. Class Separation of Lipids.
Cyanidium caldarium was cultured at 20 and 55 C and harvested during exponential growth phase. Comparative lipid studies on each cell type show a decrease by one-half of the total lipid in cells grown at 55 C over cells grown at 20 C. While the distribution of lipid into each of five lipid classes was not influenced by high temperature (55 C), the degree of unsaturation was greatly affected. Ratios of unsaturated to saturated fatty acids in these cells decreased 3-fold with increased temperature in the growth environment. Cells cultured at 20 C contained 30% of their fatty acids as linolenic while this fatty acid could not be detected in cells cultured at 55 C.
Kleinschmidt
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Division of Biochemistry, University Station, Box 3354, University of Wyoming, Laramie, Wyoming 82070.
McMahon
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