title: Protein-protein interactions in actin-myosin binding and structural effects of R405Q mutation: a molecular dynamics study.
abstract: Detailed residue-wise interactions involved in the binding of myosin to actin in the rigor conformation without nucleotides have been examined using molecular dynamics simulations of the chicken skeletal myosin head complexed with two actin monomers, based on the cryo-microscopic model of Holmes et al. (Nature 2003;425:423-427). The overall interaction is largely electrostatic in nature, because of the charged residues in the four loops surrounding the central primary binding site. The 50k/20k loop, disordered in crystal structures and in simulations of free myosin in solution, was found to be in a conformation stabilized with 1 - 2 internal salt bridges. The cardiomyopathy loop forms 2 - 3 interprotein salt bridges with actin monomers upon binding, whereas its Arg405 residue, the mutation site associated with the hypertrophic cardiomyopathy, forms a strong salt bridge with Glu605 in the neighboring helix away from actin in the actin-bound myosin. The myopathy loop of the R405Q mutant maintains a high degree of two-strand beta-sheet character when bound to actin with the corresponding salt bridges broken.
authors.affiliation: Department of Chemistry, University of Nevada, Reno 89557, USA.
authors.forename: Yuemin
authors.initials: Y
authors.lastname: Liu
authors.forename: Megan
authors.initials: M
authors.lastname: Scolari
authors.forename: Wonpil
authors.initials: W
authors.lastname: Im
authors.forename: Hyung-June
authors.initials: HJ
authors.lastname: Woo
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D019943:Amino Acid Substitution; D001665:Binding Sites; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009218:Myosins / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000199:Actins; D011994:Recombinant Proteins; D009218:Myosins