Polymorphism of the renin-angiotensin-aldosterone system in patients with chronic allograft dysfunction.
Polymorphism of the gene encoding components of the renin-angiotensin-aldosterone synthase system (RAAS) represents an area of intense research of cardiovascular disease associations. Numerous studies have addressed the role of RAAS gene polymorphisms in the development and progression of renal disease. Also, it has been reported that patient with ACE (DD) and angiotensinogen AGT (TT) genotypes are associated with chronic allograft dysfunction (CAD). We investigated the effects of gene polymorphisms of the renin-angiotensin-aldosterone system in renal transplant patients (81 males and 50 females; mean age 29.6+/-10.2 years). Genotypes were determined using polymerase chain reaction sequence specific primers and PCR followed by RFLP analysis. Renal allograft recipients with chronic allograft dysfunction had significantly higher frequencies of the MM genotype than those without CAD (P<0.05). The other genetic polymorphisms of the RAAS were not associated with CAD. This study proves that determination of AGT M235T genotype before transplantation may help identify patients who are at risk for chronic renal transplant dysfunction.
Ayed
Kh
Kh
Immunology Laboratory EPS. Ch. Nicolle Hospital, Tunis, Tunisia. khaled.ayed@rns.tn
Ayed-Jendoubi
S
S
Ben Abdallah
T
T
Bardi
R
R
Gorgi
Y
Y
Sfar
I
I
Dhrif
B
B
Abderrahim
E
E
Kheder
A
A
D000328:Adult; D000808:Angiotensinogen / Q000235:genetics; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics; D005260:Female; D006085:Graft Survival / Q000235:genetics*; D006801:Humans; D006973:Hypertension; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics; D012084:Renin-Angiotensin System / Q000235:genetics*; D014184:Transplantation, Homologous
D044140:Receptor, Angiotensin, Type 1; D000808:Angiotensinogen; D019405:Cytochrome P-450 CYP11B2; D007703:Peptidyl-Dipeptidase A