Semi-anaerobic growth conditions are favoured by some Escherichia coli strains during heterologous expression of some archaeal proteins.
Host cell physiology is known to play a crucial role in the expression of foreign genes in heterologous systems. Expression of archaeal genes in anaerobic or semi-anaerobic growth conditions of E. coli has been previously reported to be a means of improving solubility of some proteins. Here, we report that some of the Rosetta strains of E. coli, which harbour the rare tRNA genes for the expression of archaeal genes, favour semi-anaerobic conditions for the expression of putative FMN binding domain of glutamate synthase from Methanocaldococcus jannaschii at low inducer concentrations.
Demir
Volkan
V
Department of Molecular Biology and Genetics Faculty of Sciences and Letters, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.
Dincturk
H Benan
HB
D000693:Anaerobiosis; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D015870:Gene Expression; D005970:Glutamate Synthase / Q000235:genetics / Q000378:metabolism; D017015:Methanococcales / Q000201:enzymology / Q000235:genetics
D019843:Archaeal Proteins; D005970:Glutamate Synthase