title: Retinal bipolar cell types differ in their inventory of ion channels.
abstract: Bipolar cells were recorded in rat retinal slices to study the distribution of hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels. Patch-clamp whole cell measurements were combined with intracellular filling and recorded cells were morphologically identified. HCN channel isoforms HCN1-4 are differentially expressed in bipolar cells. Each bipolar cell type has a characteristic inventory of HCN channels. The combination of HCN channel currents and other voltage-gated currents can be used as a kind of "finger print" to electrophysiologically identify and classify bipolar cell types. Using this approach of combined electrophysiological and morphological classification we could identify a new ON-cone bipolar cell type.
authors.affiliation: Institut für Biologische Informationsverarbeitung 1, Forschungszentrum Jülich, Jülich, Germany.
authors.forename: Elena
authors.initials: E
authors.lastname: Ivanova
authors.forename: Frank
authors.initials: F
authors.lastname: Müller
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology; D002586:Cesium / Q000494:pharmacology; D003035:Cobalt / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods; D005998:Glycine / Q000378:metabolism; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000502:physiology*; D007473:Ion Channels / Q000145:classification* / Q000187:drug effects / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D018408:Patch-Clamp Techniques / Q000379:methods; D051381:Rats; D051245:Retinal Bipolar Cells / Q000145:classification* / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects; D050598:Vesicular Glutamate Transport Protein 1 / Q000378:metabolism
chemical_list: D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels; D050598:Vesicular Glutamate Transport Protein 1; D002586:Cesium; D001464:Barium; D003035:Cobalt; D005998:Glycine