title: Intravital microscopy of pulmonary microcirculation after single lung transplantation in pigs.
abstract: BACKGROUND
  Pulmonary reperfusion injury is a significant risk factor following lung transplantation (LTx). Unfortunately, in vivo observations and quantitative analyses of the pulmonary microcirculation following LTx are technically demanding.
  
  
  METHODS
  Pigs, weighing 18 to 22 kg, served as the laboratory animals. The left lung was harvested and preserved using donor aortic vessel segments, the pulmonary artery, and the cuff of the lung veins were extended. After 4 hours of ischemia, the lungs were transplanted by direct connection of the conduits to the left atrial appendage and the left pulmonary artery of the recipient. The lungs were placed extrathoracically and ventilated. The recipient left lung was excluded. With this procedure, mechanical trauma to the lung and moving artefacts were avoided. Intravital microscopic observation became feasible.
  
  
  RESULTS
  Following reperfusion, oxygenation of pulmonary venous blood was excellent. However, blood flow distribution was significantly reduced to the transplanted lung compared with the native right recipient lung. Pulmonary vascular resistance was significantly increased, dropping from 3500 to 1000 dynes x s x cm(-5) during reperfusion compared to a value of 500 for the native right lung. The pulmonary microcirculation showed a significant number of no-reflow areas with extremely reduced red blood cell velocities. Greater than 90% of microvessels (<30 microm) showed velocities below 0.1 mm/sec. In conclusion, microvascular injury seems to be a major pathogenic factor for the development reperfusion failure. Quantification of alterations within the microvasculature may shed light on various treatment modalities that reduce perfusion failure.
authors.affiliation: Department of Cardiac Surgery, University of Heidelberg, Heidelberg, Germany. falk-udo.sack@urz.uni-heidelberg.de
authors.forename: F-U
authors.initials: FU
authors.lastname: Sack
authors.forename: R
authors.initials: R
authors.lastname: Dollner
authors.forename: B
authors.initials: B
authors.lastname: Reidenbach
authors.forename: A
authors.initials: A
authors.lastname: Koch
authors.forename: M M
authors.initials: MM
authors.lastname: Gebhard
authors.forename: S
authors.initials: S
authors.lastname: Hagl
mesh_terms: D000818:Animals; D016040:Lung Transplantation / Q000473:pathology*; D008833:Microcirculation; D008853:Microscopy / Q000379:methods; D023421:Models, Animal; D011652:Pulmonary Circulation; D015424:Reperfusion; D013552:Swine; D020858:Tissue and Organ Harvesting / Q000379:methods