High-mannose-type glycan modifications of dihydrofolate reductase using glycan-methotrexate conjugates.
Various high-mannose-type glycan modifications of dihydrofolate reductase (DHFR) were achieved by ligand-based approach using glycan-methotrexate (MTX) conjugates as tight binding glycan bearing ligands for DHFR. The resulting glycan-MTX conjugates and the corresponding artificial glycoproteins could be useful as oligosaccharide- and glycoprotein-probes to perform quantitative analysis of glycan recognizing protein such as lectins, glycosyltransferases or glycosidases. Moreover, artificial glycoproteins having two different high-mannose-type glycans were developed for the first time by a combination of two different types of glycan modification strategies.
Totani
Kiichiro
K
RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Matsuo
Ichiro
I
Ihara
Yoshito
Y
Ito
Yukishige
Y
D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D008024:Ligands; D008358:Mannose / Q000737:chemistry*; D008727:Methotrexate / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000187:drug effects*
D008024:Ligands; D011134:Polysaccharides; D013762:Tetrahydrofolate Dehydrogenase; D008358:Mannose; D008727:Methotrexate