title: Activity of kk-42 in combinated treatment with RH-0345 or 20-hydroxyecdysone on morphometric measurements and free ecdysteroid in eggs of mealworms.
abstract: RH-0345 (halofenozide), a bisacylhydrazine derivative, is a nonsteroidal ecdysteroid agonist that mimics the action of the moulting hormones, while KK-42, an imidazole compound, is a potent inhibitor of ecdysteroid biosynthesis. Recent experiments with Tenebrio molitor suggested that the reduction of ecdysteroid titer, leading to a reduction of reproductive capacity, is due to a direct and rapid action of KK-42 on ecdysterold biosynthesis. Moreover, RH-0345 could partly restore the effects on reproductive events induced by KK-42. On the other hand, RH-0345 was found to affect growth and development of ovaries in a manner similar to that 20-hydroxyecdysone (20-E) and increased the ecdysteroid production. Therefore, the present study evaluates KK-42 applied topically (10 microg/insect) in combination with RH-0345 or 20-E in order to obtain more information on the mode of action of this compound on reproduction in T. molitor. In a first series of experiments, the compounds were assayed on morphometric measurements of freshly laid eggs. Results showed that both the weight and the volume of eggs increase significantly in series treated by KK-42 followed by 20-hydroxyecdysone as compared to controls and treated series by KK-42 followed by RH-0345. Data from enzyme immunoassay measurements revealed that KK-42 applied before 20-E was found to reduce significantly the amounts of free ecdysteroids in eggs comparatively to the others series.
authors.affiliation: Laboratoire de Biologie Animale Appliquée, Département de Biologie Faculté des Sciences, Université d'Annaba, 23000-Annaba, Algérie.
authors.forename: H
authors.initials: H
authors.lastname: Berghiche
authors.forename: M
authors.initials: M
authors.lastname: Houamria
authors.forename: G
authors.initials: G
authors.lastname: Smagghe
authors.forename: N
authors.initials: N
authors.lastname: Soltani
mesh_terms: D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D004338:Drug Combinations; D026461:Ecdysteroids / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D004441:Ecdysterone / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006834:Hydrazines / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D007605:Juvenile Hormones / Q000494:pharmacology*; D009866:Oogenesis / Q000187:drug effects; D010058:Oviposition / Q000187:drug effects; D013712:Tenebrio / Q000187:drug effects* / Q000502:physiology
chemical_list: D001565:Benzoates; D004338:Drug Combinations; D026461:Ecdysteroids; D006834:Hydrazines; D007093:Imidazoles; D007605:Juvenile Hormones; D004441:Ecdysterone; C063652:1-benzyl-5-(2,6-dimethyl-1,5-heptadienyl)imidazole; C437546:N-4-chlorobenzoyl-N'-benzoyl-N'-tert-butylhydrazine