[Comparison of nucleotide and deduced amino acid sequences of E gene of the newly isolated Japanese encephalitis virus strains and inactivated vaccine strain P3].
BACKGROUND
To analyze the difference of nucleotides and deduced amino acids sequences E gene between the newly isolated Japanese encephalitis (JE) virus strains from mosquitoes or patients and P3 strain.


METHODS
The E gene sequences of corresponding strains of JE virus were obtained from GenBank. Computer analyze of nucleic acid data and deduced amino acid sequence were accomplished using the Clustal X (1.8), DNASTAR, GENEDOC (3.2) programs.


RESULTS
The result showed that compared with the Fujian strains and P3 strain the nucleotide sequence homology was up to 98.3%, and the amino acid sequence homology was up to 98.2%, respectively. Compared with the Shanghai strains and P3 strain, the nucleotide sequence differences were 12%, and the amino acid sequence homology was up to 98.2%, respectively. Compared with P3 strain, there were nineteen amino acid variations in E gene of all the newly isolated strains. Between P3 and all the newly isolated JE virus strains, there are three common variations at E-129, E-222, E-366. And two common variations E-160 and E-487 were found only in Fujian strains, common variations at E-129, E-222, E-227, E-366 in Shanghai strains.


CONCLUSIONS
There are some differences between P3 strain and JE viruses which were isolated from mosquitoes belonging to genotype I in Shanghai and from patients belonging to genotype III from Fujian province. But these variations are not in the important locations affecting the biological characteristic of the viruses.
Wang
Huan-yu
HY
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Liang
Guo-dong
GD
D000595:Amino Acid Sequence; D000818:Animals; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D004672:Encephalitis, Japanese / Q000276:immunology / Q000821:virology*; D014644:Genetic Variation; D006801:Humans; D012689:Sequence Homology, Nucleic Acid; D015164:Vaccines, Inactivated; D014759:Viral Envelope Proteins / Q000235:genetics*
D015164:Vaccines, Inactivated; D014759:Viral Envelope Proteins