title: Prefabricated engineered skin flap using an arteriovenous vascular bundle as a vascular carrier in rabbits.
abstract: BACKGROUND
  The authors previously described induction of spontaneous tissue generation by implanting a collagen matrix and a ligated pedicle (arteriovenous bundle) into a hollow porous chamber in vivo in the rabbit. They hypothesized that increased tissue volume could be obtained by the application of basic fibroblast growth factor (bFGF) and/or by increasing the chamber size and porosity.
  
  
  METHODS
  In rabbits, a saphenous arteriovenous pedicle and a collagen sponge were inserted into a porous chamber in the groin. Small-volume pore chambers (experiment 1, n = 7) and larger-volume, wider pore chambers (experiment 2, n = 13) were compared, and each was compared with and without bFGF. An additional three flaps of experiment 2 with bFGF were skin grafted, microsurgically transplanted to the ear, and evaluated at 6 months for stability.
  
  
  RESULTS
  All patent chambers grew tissue; chambers with bFGF were almost filled, and those without were only half-filled. Histomorphometric analysis confirmed a significant difference. The larger-volume, larger-pore chambers produced more than twice the volume of tissue as the smaller chambers did, and this was significant. Tissue volume in both the control and bFGF groups of experiment 2 was significantly greater than that in the respective groups of experiment 1. Histology, angiography, and scanning electron microscopy confirmed greater vascularity in the bFGF groups and demonstrated vascular connections penetrating the chamber pores linking with angiogenic sprouts, probably from the vasa vasorum of the pedicle, to contribute to new growth. Transplanted flaps survived and appeared normal 6 months later.
  
  
  CONCLUSIONS
  Patent pedicles, bFGF, large pore size, and larger-volume chambers all seemed to contribute to increased tissue growth in this model. The tissue is stable long term.
authors.affiliation: Division of Plastic and Reconstructive Surgery, Shinko Hospital, Kobe, Japan. yoshiman@shinkohp.or.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Ki-Chul
authors.initials: KC
authors.lastname: Sung
authors.forename: Michihiro
authors.initials: M
authors.lastname: Fumimoto
authors.forename: Akira
authors.initials: A
authors.lastname: Tsutsumi
authors.forename: Shunzo
authors.initials: S
authors.lastname: Kondo
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Hinohara
authors.forename: Wayne A
authors.initials: WA
authors.lastname: Morrison
mesh_terms: D000818:Animals; D001158:Arteries / Q000601:surgery; D003094:Collagen; D015194:Diffusion Chambers, Culture; D004431:Ear, External / Q000601:surgery; D004867:Equipment Design; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006097:Granulation Tissue / Q000648:ultrastructure; D006119:Groin; D008297:Male; D008855:Microscopy, Electron, Scanning; D008866:Microsurgery; D018919:Neovascularization, Physiologic; D011817:Rabbits; D012501:Saphenous Vein / Q000601:surgery; D016038:Skin Transplantation / Q000379:methods*; D017768:Skin, Artificial; D013524:Surgical Flaps; D015503:Surgical Sponges; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D014182:Transplantation, Autologous; D014651:Vasa Vasorum / Q000166:cytology / Q000648:ultrastructure
chemical_list: D016222:Fibroblast Growth Factor 2; D003094:Collagen