title: Full-field optical sectioning and three-dimensional localization of fluorescent particles using focal plane modulation.
abstract: We present a technique for imaging fluorescent particles based on the axial modulation of the objective's focal plane position. This technique provides full-field optical sectioning and can be used to localize the fluorophores in three dimensions. We describe the technique and apply it to image 200 nm diameter fluorescent beads immobilized in a gel. We show that full-field optical sectioning is obtained and that the beads are localized with a precision of 10 nm in the transverse plane and 14 nm in the axial direction.
authors.affiliation: Laboratoire d'Optique Physique, ESPCI, Paris, France. frederic.chasles@espci.fr
authors.forename: F
authors.initials: F
authors.lastname: Chasles
authors.forename: B
authors.initials: B
authors.lastname: Dubertret
authors.forename: A C
authors.initials: AC
authors.lastname: Boccara