Controlling DNA orientation on mixed ssDNA/OEG SAMs.
We present and characterize a mixed self-assembled monolayer (SAM) consisting of single-stranded oligonucleotide (ssDNA)- and oligo(ethylene glycol) (OEG)-terminated thiols. The ssDNA/OEG SAMs are prepared by simultaneous coadsorption from a common thiol solution over a broad range of compositions. Electron spectroscopy for chemical analysis (ESCA) is used to measure the surface coverage of ssDNA, whereas surface plasmon resonance (SPR) sensor is used to measure the hybridization of complementary ssDNA and protein resistance. Through the complementary use of these techniques, we find that the composition of OEG in the assembly solution controls a key parameter: the surface coverage of ssDNA on the surface. There is evidence that it influences the orientation of the immobilized ssDNA probes. Lower OEG concentrations yield a surface with higher ssDNA coverage and less favorable orientation, whereas higher OEG concentrations produce a surface with lower DNA coverage and more favorable orientation. Competition between these two effects controls the hybridization efficiency of the ssDNA surface. Compared to ssDNA surfaces prepared with other diluent thiols, the use of OEG improves the protein resistance of the surface, making it more broadly applicable.
Boozer
Christina
C
Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, USA.
Chen
Shengfu
S
Jiang
Shaoyi
S
D000327:Adsorption; D004277:DNA, Single-Stranded; D011092:Polyethylene Glycols / Q000737:chemistry*; D011485:Protein Binding; D012996:Solutions / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties
D004277:DNA, Single-Stranded; D012996:Solutions; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols