title: Feasibility of diffuse optical imaging with long-lived luminescent probes.
abstract: Long-lived near-infrared phosphors with high quantum yields have recently become available, making it possible to image oxygen distributions in tissue in three dimensions. By numerical simulations we demonstrate that, by using phosphorescent probes with appropriate oxygen quenching constants, one can image hypoxic phantoms in scattering media with adequate spatial resolution, employing simple time-gated measurements. The approach developed will guide experimental imaging of phosphorescence lifetime and oxygen pressure in living tissue.
authors.affiliation: Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Sofia V
authors.initials: SV
authors.lastname: Apreleva
authors.forename: David F
authors.initials: DF
authors.lastname: Wilson
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Vinogradov
mesh_terms: D015687:Cell Hypoxia / Q000502:physiology*; D003198:Computer Simulation; D005240:Feasibility Studies; D005456:Fluorescent Dyes; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D010100:Oxygen