Heterologous complementation of the Klaac null mutation of Kluyveromyces lactis by the Saccharomyces cerevisiae AAC3 gene encoding the ADP/ATP carrier.
The KlAAC gene, encoding the ADP/ATP carrier, has been assumed to be a single gene in Kluyveromyces lactis, an aerobic, petite-negative yeast species. The Klaac null mutation, which causes a respiratory-deficient phenotype, was fully complemented by AAC2, the Saccharomyces cerevisiae major gene for the ADP/ATP carrier and also by AAC1, a gene that is poorly expressed in S. cerevisiae. In this study, we demonstrate that the Klaac null mutation is partially complemented by the ScAAC3 gene, encoding the hypoxic ADP/ATP carrier isoform, whose expression in S. cerevisiae is prevented by oxygen. Once introduced into K. lactis, the AAC3 gene was expressed both under aerobic and under partial anaerobic conditions but did not support the growth of K. lactis under strict anaerobic conditions.
Fontanesi
Flavia
F
Department of Genetics, Anthropology & Evolution, University of Parma, Parco Area delle Scienze, Parma, Italy.
Viola
Anna Maria
AM
Ferrero
Iliana
I
D000693:Anaerobiosis / Q000235:genetics; D015152:Blotting, Northern; D003580:Cytochromes / Q000032:analysis; D005285:Fermentation / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D007716:Kluyveromyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics* / Q000502:physiology*; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology; D013057:Spectrum Analysis; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D003580:Cytochromes; D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D000226:Mitochondrial ADP, ATP Translocases