[Effect of fibrinogen on the adherence of Porphyromonas gingivalis to human oral epithelial cells].
OBJECTIVE
To study the role of fibrinogen molecule in the pathogenesis of periodontal diseases.


METHODS
An in vitro cell culture model was used. Methyl-(3)H Thymidine radiolabeled Porphyromonas gingivalis (Pg) ATCC 33277 were examined for their ability to adhere to and invade the confluent monolayers of human oral epithelial KB cells with or without exogenous human fibrinogens by scintillation spectrometry.


RESULTS
The addition of exogenous fibrinogens made more amount of and higher ratios of adhesive and invasive Pg, in contrast to the group without exogenous fibrinogen (P < 0.001). At different concentrations of exogenous fibrinogen, the amount and ratios of adhesive and invasive Pg varied significantly (P < or = 0.007). The higher concentrations of exogenous fibrinogen was added, the greater amount and ratios of adhesive and invasive Pg were found.


CONCLUSIONS
Fibrinogen promotes the adherence of Pg to human oral epithelial cells and may play an important role in the pathogenesis of periodontal diseases.
Ge
Song
S
Department of Periodontology, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Wu
Ya-fei
YF
Liu
Tian-jia
TJ
Zhao
Lei
L
Fei
Xiao-lu
XL
D001422:Bacterial Adhesion / Q000187:drug effects; D005340:Fibrinogen / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D007624:KB Cells; D009061:Mouth Mucosa / Q000187:drug effects* / Q000382:microbiology; D010518:Periodontitis / Q000209:etiology*; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*
D005340:Fibrinogen