Vitamin B6 suppresses growth of the feline mammary tumor cell line FRM.
Growth of FRM cells was inhibited by the addition of pyridoxine in a dose-dependent manner. Use of 5 mM pyridoxine caused an almost complete arrest of cell growth. Pyridoxal was as effective as pyridoxine, but pyridoxamine showed weak inhibitory action. Electron-microscopic examination of control cells revealed large nuclei and cellular membranes with villi, but, in pyridoxine-treated cells, condensed or degraded nuclei were observed. Many vacuoles and cholesterol crystals were widely distributed inside the cellular membrane of pyridoxine-treated cells. One of the vacuoles was identified as a lipid droplet. The DNA ladder was observed in the pyridoxine-treated cells. It is suggested that pyridoxine treatment of FRM cells causes cytolysis of cells by apoptosis.
Shimada
Dai
D
Department of Veterinary Physiology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan.
Fukuda
Ayumi
A
Kanouchi
Hiroaki
H
Matsumoto
Mitsuharu
M
Oka
Tatsuzo
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D002415:Cats; D045744:Cell Line, Tumor; D008854:Microscopy, Electron; D025101:Vitamin B 6 / Q000494:pharmacology*
D025101:Vitamin B 6