Loss of heterozygosity analysis of a candidate gastric carcinoma tumor suppressor locus at 7q31.
Gastric carcinoma is one of the most common malignancies in Asia. Although the allelic deletion of 7q has been reportedly associated with primary gastric carcinoma tumorigenesis, no predisposing genes in this region have been identified so far. Here, we report the results of genotype and loss of heterozygosity (LOH) analysis on 7q in this tumor. A panel of nine microsatellite markers distributed over the whole chromosome 7q was used for genotyping primary gastric carcinomas. Of 72 primary tumors LOH of D7S486 occurred in 24.0% (12/50) of cases. Fine mapping with 12 additional markers flanking D7S486 resulted in LOH of 30.36% (17/56) and defined one minimal deleted region in primary gastric carcinomas, a 90-kilobase region bounded by D7S2543 and D7S486 at 7q31.2. The allelic deletion correlates statistically with clinicopathologic variables. Our data suggest a possible link between putative tumor suppressor genes and gastric carcinoma in the 7q31 region.
Xia
Jian-Chuan
JC
Research Section of Oncopathology, State Key Laboratory of Oncology in Southern China, Cancer Center, Zhongshan University, 651 Dong-feng Road East, Guangzhou 510060, China.
Weng
De-Sheng
DS
Li
Jin-Tian
JT
Qin
Hai-De
HD
Mai
Shi-Juan
SJ
Feng
Bing-Jian
BJ
Fan
Qin
Q
Feng
Qi-Sheng
QS
Huang
Li-Xi
LX
Yu
Xing-Juan
XJ
Pan
Zhi-Zhong
ZZ
Li
Yong-Qiang
YQ
Wang
Qi-Jing
QJ
Zhan
You-Qing
YQ
Chen
Shi-Ping
SP
He
Jia
J
Huang
Wen-lin
WL
Wu
Pei-Hong
PH
Zeng
Yi-Xing
YX
D000328:Adult; D000368:Aged; D002872:Chromosome Deletion; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D020161:Physical Chromosome Mapping; D013274:Stomach Neoplasms / Q000235:genetics*