title: Identification of DNA copy-number aberrations by array-comparative genomic hybridization in patients with schizophrenia.
abstract: Chromosomal abnormalities are implicated as important markers for the pathogenesis in patients with schizophrenia. In this study, with using bacterial artificial chromosome (BAC) array-based comparative genomic hybridization (CGH), we analyzed DNA copy-number changes among 30 patients with schizophrenia. The most frequent changes were partial gain of Xq23 (52%) and loss of 3q13.12 (32%). Other frequent gains were found in: 1p, 6q, 10p, 11p, 11q, 14p, and 15q regions, and frequent losses were found in: 2p, 9q, 10q, 14q, 20q, and 22q regions. The set of abnormal regions was confirmed by real-time PCR (9q12, 9q34.2, 11p15.4, 14q32.33, 15q15.1, 22q11.21, and Xq23). All real-time PCR results were consistent with the array-CGH results. Therefore, it is suggested that array-CGH and real-time PCR analysis could be used as powerful tools in screening for schizophrenia-related genes. Our results might be useful for further exploration of candidate genomic regions in the pathogenesis of schizophrenia.
authors.affiliation: Department of Pharmacology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: Moon
authors.forename: Sung-Vin
authors.initials: SV
authors.lastname: Yim
authors.forename: Woon Kyu
authors.initials: WK
authors.lastname: Lee
authors.forename: Yang-Whan
authors.initials: YW
authors.lastname: Jeon
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Ko
authors.forename: Kwang-Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Kweon-Haeng
authors.initials: KH
authors.lastname: Lee
authors.forename: Sang-Ick
authors.initials: SI
authors.lastname: Han
authors.forename: Hyoung Kyun
authors.initials: HK
authors.lastname: Rha
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations / Q000706:statistics & numerical data*; D002874:Chromosome Mapping / Q000379:methods*; D004252:DNA Mutational Analysis / Q000379:methods*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D015994:Incidence; D007723:Korea / Q000453:epidemiology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012559:Schizophrenia / Q000453:epidemiology* / Q000235:genetics*