Chemotherapy induces an increase in coenzyme Q10 levels in cancer cell lines.
Free radicals have been implicated in the action of many chemotherapeutic drugs. Here we tested the hypothesis that camptothecin and other chemotherapeutic drugs, such as etoposide, doxorubicin, and methotrexate, induce an increase in coenzyme Q(10) levels as part of the antioxidant defense against free radical production under these anticancer treatments in cancer cell lines. Chemotherapy treatment induced both free radical production and an increase in coenzyme Q(10) levels in all the cancer cell lines tested. Reduced coenzyme Q(10) form levels were particularly enhanced. Coenzyme Q(10)-increased levels were associated with up-regulation of COQ genes expression, involved in coenzyme Q(10) biosynthesis. At the translational level, COQ7 protein expression levels were also increased. Furthermore, coenzyme Q(10) biosynthesis inhibition blocked camptothecin-induced coenzyme Q(10) increase, and enhanced camptothecin cytotoxicity. Our findings suggest that coenzyme Q(10) increase is implicated in the cellular defense under chemotherapy treatment and may contribute to cell survival.
Brea-Calvo
Gloria
G
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, Carretera de Utrera Km 1, Sevilla, Spain.
Rodríguez-Hernández
Angeles
A
Fernández-Ayala
Daniel J M
DJ
Navas
Plácido
P
Sánchez-Alcázar
José A
JA
D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D002166:Camptothecin / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D003067:Coenzymes; D005609:Free Radicals / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D010084:Oxidation-Reduction / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D015854:Up-Regulation / Q000187:drug effects
D000970:Antineoplastic Agents; D003067:Coenzymes; D005609:Free Radicals; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D014451:Ubiquinone; C024989:coenzyme Q10; D002166:Camptothecin