Modulatory effect of fish oil on the myocardial antioxidant defense system in isoproterenol-induced myocardial infarction.
The effect of fish oil treatment on the activities of antioxidants such as superoxide dismutase, catalase, glutathione peroxidase, glutathione-s-transferase, and glutathione, as well as the level of the lipid peroxidation marker thiobarbituric reactive substance was studied in isoproterenol-induced myocardial infarction (MI). To confirm the induction of MI by isoproterenol, we studied the activities of cardiac marker enzymes like creatinine kinase and lactate dehydrogenase and the level of troponin. The biochemical lesions due to the activation of lipid peroxidation and decrease in antioxidant status are significantly implicated in experimental isoproterenol-induced MI. The results indicate that the protective effect of fish oil is achieved by decreasing the peroxide concentration and normalizing antioxidant defense enzymes.
Padma
V Vijaya
VV
Department of Biochemistry and Molecular Biology, University of Madras, Guindy Campus, Chennai 600 025, India. vvijayapadma@rediffmail.com
Devi
C S Shyamala
CS
Ramkumar
K M
KM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D002570:Ceruloplasmin / Q000032:analysis / Q000378:metabolism; D003402:Creatine Kinase / Q000097:blood / Q000378:metabolism; D004247:DNA / Q000032:analysis; D005395:Fish Oils / Q000494:pharmacology*; D005978:Glutathione / Q000032:analysis / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007501:Iron / Q000032:analysis / Q000097:blood / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000378:metabolism; D008054:Lipid Peroxides / Q000032:analysis / Q000097:blood / Q000378:metabolism; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D009206:Myocardium / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D020107:Troponin T / Q000097:blood
D000975:Antioxidants; D005395:Fish Oils; D008054:Lipid Peroxides; D011506:Proteins; D020107:Troponin T; D012313:RNA; D004247:DNA; D007501:Iron; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D005982:Glutathione Transferase; D003402:Creatine Kinase; D005978:Glutathione; D007545:Isoproterenol