Alleviation of picryl chloride-induced delayed type hypersensitivity reaction by saponin fraction of Gleditsia sinensis.
The objective of the present study was to investigate the effects of saponin fraction from anomalous fruits of Gleditsia sinensis (SFGS) on picryl chloride-induced delayed type hypersensitivity (PC-DTH) and functions of T lymphocytes and macrophages in mice. SFGS (100, 200 mg/kg), orally administered during either sensitization stage or effector stage, produced remarkable inhibition of PC-DTH. In vitro, SFGS (1, 2, 4 microg/ml) concentration-dependently attenuated concanavalin A (Con A)-elicited mouse splenocyte proliferation and interleukin 2 (IL-2) production. At concentrations of 10 and 20 microg/ml, SFGS inhibited lipopolysaccharide (LPS)-induced production of nitric oxide and interleukin 1beta (IL-1beta) of mouse peritoneal macrophages. The findings indicate that SFGS attenuates PC-DTH in mice, which is probably mediated by preventing proliferation and differentiation of T cells during the sensitization stage and suppressing activation of macrophages during the effector stage.
Hou
Li-Fei
LF
Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing.
Dai
Yue
Y
Xia
Yu-Feng
YF
Gong
Zhu-Nan
ZN
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D031501:Gleditsia / Q000737:chemistry*; D006968:Hypersensitivity, Delayed / Q000188:drug therapy*; D007202:Indicators and Reagents; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000096:biosynthesis; D010853:Picryl Chloride / Q000009:adverse effects*; D012503:Saponins / Q000627:therapeutic use*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D007202:Indicators and Reagents; D007375:Interleukin-1; D007376:Interleukin-2; D008070:Lipopolysaccharides; D012503:Saponins; D003208:Concanavalin A; D009569:Nitric Oxide; D010853:Picryl Chloride