title: The interaction mode of premalignant Schwann and immune effector cells during chemically induced carcinogenesis in the rat peripheral nervous system is strongly influenced by genetic background.
abstract: Contrary to rats of the highly sensitive inbred strain BDIX, BDIV rats are resistant to the induction of malignant schwannomas by N-ethyl-N-nitrosourea, arising predominantly in the trigeminal nerves. A point mutation of the neu/erbB-2 gene diagnostic of N-ethyl-N-nitrosourea-induced rat schwannomas is an early marker of Schwann precursor cells at high risk of subsequent malignant transformation. Neu-mutant cells initially arise at a similar frequency in sensitive and resistant animals. However, these cells disappear from the trigeminal nerves of resistant rats while giving rise to highly malignant schwannomas in susceptible animals. The resistance of BDIV rats obviously includes mechanisms to recognize and eliminate premalignant cells. The involvement of a cellular immune response was investigated in trigeminal nerves of both strains at different times after neonatal carcinogen exposure. An inflammatory reaction involving sequentially CD4(+) macrophages and T helper cells, CD8(+) cytotoxic T cells, and ED1(+) and ED2(+) macrophages was detected as a consequence of N-ethyl-N-nitrosourea treatment as early as postnatal day 40, briefly after the emergence of premalignant neu-mutant Schwann cells. It persisted throughout the observation period (40-250 days). However, there were no gross differences in immune cell counts between tumor-susceptible and tumor-resistant rats, except for a moderate increase of ED2(+) macrophages in N-ethyl-N-nitrosourea-treated BDIX rats only. Differential interactions of immune effector cells with premalignant Schwann cells may thus be involved in genetically determined tumor susceptibility or resistance, which could include functional differences of immune effector cells and/or a differential capability of premalignant Schwann cells to escape or counteract the cellular immune response.
authors.affiliation: Institute of Cell Biology (Cancer Research), University of Duisburg-Essen Medical School and West German Cancer Center Essen, Essen, Germany.
authors.forename: Katharina M
authors.initials: KM
authors.lastname: Gering
authors.forename: Judith A M
authors.initials: JA
authors.lastname: Marx
authors.forename: Klaus
authors.initials: K
authors.lastname: Lennartz
authors.forename: Christine
authors.initials: C
authors.lastname: Fischer
authors.forename: Manfred F
authors.initials: MF
authors.lastname: Rajewsky
authors.forename: Andrea
authors.initials: A
authors.lastname: Kindler-Röhrborn
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D018821:CD18 Antigens / Q000276:immunology; D002450:Cell Communication / Q000235:genetics* / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D003390:Cranial Nerve Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D005038:Ethylnitrosourea; D007150:Immunohistochemistry; D007962:Leukocytes / Q000276:immunology / Q000473:pathology*; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D009442:Neurilemmoma / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D011230:Precancerous Conditions / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000473:pathology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000473:pathology; D014276:Trigeminal Nerve / Q000187:drug effects / Q000473:pathology
chemical_list: D000906:Antibodies; D018821:CD18 Antigens; D005038:Ethylnitrosourea