Feasibility of using collagen as the base of the antifungal drug, miconazole.
The feasibility of using collagen as the base of miconazole was investigated. The addition of 33% collagen to a miconazole solution did not affect the minimal inhibitory concentration (MIC80) of the miconazole solution for Candida albicans. When 1 microg mL(-1) of miconazole in 33% collagen solution was plated on resin discs and dried to yield a thin membrane, the growth of C. albicans on the resin discs was nearly completely inhibited. In addition, we compared the antifungal effect of this collagen solution that contained 1 microg mL(-1) miconazole, with the antifungal effect of miconazole gel that had been diluted with glycerol (the main component of miconazole gel) to yield a final concentration of 1 microg mL(-1) of miconazole; as a result, we found that the collagen solution containing 1 microg mL(-1) miconazole had a stronger antifungal effect. In conclusion, our results demonstrated that it may be feasible to use collagen as the base of miconazole instead of glycerol, and suggest that a collagen-based miconazole solution would have a stronger antifungal effect than commercially available miconazole gel. Collagen-based miconazole solution may be useful for the treatment of Candida-associated denture stomatitis.
Yoshida
K
K
Department of Removable Prosthodontics, Matsumoto Dental University, Nagano, Japan.
Hirai
K
K
Ara
T
T
Ito
M
M
Wang
P-L
PL
Igarashi
Y
Y
D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D003094:Collagen / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004337:Drug Carriers / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D005240:Feasibility Studies; D006801:Humans; D008825:Miconazole / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods
D000935:Antifungal Agents; D004337:Drug Carriers; D008825:Miconazole; D003094:Collagen