Effects of bone marrow stromal cell injection in an experimental glaucoma model.
We investigated if bone marrow stromal cells (BMSCs) transplanted into the vitreous body of a glaucoma model eye could be integrated in the host retina and also whether they could rescue the retinal ganglion cells (RGCs) from death induced by the elevated intraocular pressure. Glaucoma was induced in the right eye of adult Wistar rats by ligating the episcleral veins. The GFP-expressing BMSCs (GFP-BMSCs) were injected into the vitreous body of both the control and the glaucomatous eyes. After transplantation, GFP-BMSCs were mostly present along with the inner limiting membrane and only a few cells were integrated into the ganglion cell layer. At 2 or 4 weeks after transplantation, GFP-BMSCs were observed to express various trophic factors. The BMSCs injected glaucoma model eyes showed less reduction in the number of RGCs compared to the glaucomatous eyes with PBS injection. This study suggests that BMSC transplantation may be worthy as a neuroprotective tool to treat glaucoma.
Yu
Saiyuu
S
Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Tanabe
Teruyo
T
Dezawa
Mari
M
Ishikawa
Hiroto
H
Yoshimura
Nagahisa
N
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D004195:Disease Models, Animal; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism; D005901:Glaucoma / Q000628:therapy*; D049452:Green Fluorescent Proteins / Q000032:analysis; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D017154:Stromal Cells / Q000637:transplantation
D009414:Nerve Growth Factors; D049452:Green Fluorescent Proteins; D005346:Fibroblast Growth Factors; D017228:Hepatocyte Growth Factor