Real-time detection of mesothelin in pancreatic cancer cell line supernatant using an acoustic wave immunosensor.
BACKGROUND
An acoustic wave immunosensor was developed to illustrate the viability of such devices in early detection of molecular cancer biomarkers. The methods described here involve a real-time, less invasive technique for detecting mesothelin, a protein that has been linked to pancreatic and ovarian cancer.


METHODS
Antibodies were immobilized on the gold surface of the device via a self-assembled alkanethiol monolayer. Supernatant from two different pancreatic cancer cell-lines (PL1 and CAPAN2) containing an unknown concentration of mesothelin was tested for the protein by a flow-through analytical technique in three types of experiments. Binding of the mesothelin to the immobilized antibody layer caused a shift in the device's resonant frequency, which was correlated to the concentration of supernatant. A reference sensor was used to correct for frequency shifts caused by pressure or viscosity effects from the injection of the supernatant solution.


RESULTS
Repeated experiments indicate that the sensors are capable of nanogram detection thresholds of mesothelin proteins at room temperature and in complex mixture.


CONCLUSIONS
Acoustic wave device biosensors have the potential to become a valuable tool in screening for pancreatic as well as other types of cancers. The main features include real-time detection, high sensitivity, and ease of use.
Corso
Christopher D
CD
Department of Biomedical Engineering, Georgia Institute of Technology, 791 Atlantic Drive, Atlanta, GA 30332, USA.
Stubbs
Desmond D
DD
Lee
Sang-Hun
SH
Goggins
Michael
M
Hruban
Ralph H
RH
Hunt
William D
WD
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D003199:Computer Systems; D058851:GPI-Linked Proteins; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology; D000090204:Mesothelin; D009991:Oscillometry; D010190:Pancreatic Neoplasms / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015415:Biomarkers; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D000090204:Mesothelin