title: Polarisation, key to good localisation.
abstract: Polarisation of cells is crucial for vectorial transport of ions and solutes. In literature, however, proteins specifically targeted to the apical or basolateral membrane are often studied in non-polarised cells. To investigate whether these data can be extrapolated to expression in polarised cells, we studied several membrane-specific proteins. In polarised MDCK cells, the Aquaporin-2 water channel resides in intracellular vesicles and apical membrane, while the vasopressin-type 2 receptor, anion-exchanger 1 (AE1) protein and E-Cadherin mainly localise to the basolateral membrane. In non-polarised MDCK cells, however, Aquaporin-2 localises, besides plasma membrane, mainly in the Golgi complex, while the others show a dispersed staining throughout the cell. Moreover, while AQP2 mutants in dominant nephrogenic diabetes insipidus are missorted to different organelles in polarised cells, they all predominantly localise to the Golgi complex in non-polarised MDCK cells. Additionally, the maturation of V2R, and likely its missorting, is affected in transiently-transfected compared to stably-transfected cells. In conclusion, we show that the use of stably-transfected polarised cells is crucial in interpreting the processing and the localisation of membrane targeted proteins.
authors.affiliation: Department of Physiology, Nijmegen Center for Molecular Life Science, Radboud University Nijmegen Medical Centre, Rm 7.83, PO Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: Moniek
authors.initials: M
authors.lastname: van Beest
authors.forename: Joris H
authors.initials: JH
authors.lastname: Robben
authors.forename: Paul J M
authors.initials: PJ
authors.lastname: Savelkoul
authors.forename: Giel
authors.initials: G
authors.lastname: Hendriks
authors.forename: Mark A J
authors.initials: MA
authors.lastname: Devonald
authors.forename: Irene B M
authors.initials: IB
authors.lastname: Konings
authors.forename: Anne K
authors.initials: AK
authors.lastname: Lagendijk
authors.forename: Fiona
authors.initials: F
authors.lastname: Karet
authors.forename: Peter M T
authors.initials: PM
authors.lastname: Deen
mesh_terms: D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D051399:Aquaporin 2 / Q000235:genetics / Q000378:metabolism*; D019556:COS Cells; D015820:Cadherins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D002522:Chlorocebus aethiops; D018500:Diabetes Insipidus, Nephrogenic / Q000378:metabolism / Q000473:pathology; D004285:Dogs; D006056:Golgi Apparatus / Q000378:metabolism; D009154:Mutation; D015388:Organelles / Q000378:metabolism; D017483:Receptors, Vasopressin / Q000378:metabolism*; D014162:Transfection
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D051399:Aquaporin 2; D015820:Cadherins; D017483:Receptors, Vasopressin