Experimental design considerations in quantification experiments by using the internal standard technique: cholesterol determination by gas chromatography as a case study.
An overview on experimental design in quantification experiments using the internal standard technique is described. Particular emphasis is given to the modelling of the relationship analyte/internal standard and the estimation of the optimal amount of internal standard to be used throughout the analytical concentration range by using a uniform shell design with a minimum number of seven experiments. The described approach is demonstrated in the analysis of cholesterol in various reference materials by gas chromatography (GC), using alpha-5 cholestan as internal standard. The outlined approaches can be applied in the determination of any kind of analyte.
Araujo
Pedro
P
National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 2029 Nordnes, N-5817 Bergen, Norway. pedro.araujo@nifes.no
Couillard
Felicia
F
Leirnes
Else
E
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D002784:Cholesterol / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D012015:Reference Standards
D002784:Cholesterol