title: Cross-talk towards the response regulator NtrC controlling nitrogen metabolism in Rhodobacter capsulatus.
abstract: Rhodobacter capsulatus NtrB/NtrC two-component regulatory system controls expression of genes involved in nitrogen metabolism including urease and nitrogen fixation genes. The ntrY-ntrX genes, which are located immediately downstream of the nifR3-ntrB-ntrC operon, code for a two-component system of unknown function. Transcription of ntrY starts within the ntrC-ntrY intergenic region as shown by primer extension analysis, but maximal transcription requires, in addition, the promoter of the nifR3-ntrB-ntrC operon. While ntrB and ntrY single mutant strains were able to grow with either urea or N2 as sole nitrogen source, a ntrB/ntrY double mutant (like a ntrC-deficient strain) was no longer able to use urea or N2. These findings suggest that the histidine kinases NtrB and NtrY can substitute for each other as phosphodonors towards the response regulator NtrC.
authors.affiliation: Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Jülich, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Drepper
authors.forename: Jessica
authors.initials: J
authors.lastname: Wiethaus
authors.forename: Daphne
authors.initials: D
authors.lastname: Giaourakis
authors.forename: Silke
authors.initials: S
authors.lastname: Gross
authors.forename: Britta
authors.initials: B
authors.lastname: Schubert
authors.forename: Markus
authors.initials: M
authors.lastname: Vogt
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Wiencek
authors.forename: Alastair G
authors.initials: AG
authors.lastname: McEwan
authors.forename: Bernd
authors.initials: B
authors.lastname: Masepohl
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016354:Rhodobacter capsulatus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014508:Urea / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D015534:Trans-Activators; D014508:Urea; D011494:Protein Kinases; C055699:protein kinase-phosphatase NTRB; D010749:Phosphoprotein Phosphatases; D009584:Nitrogen