title: Potyvirus terminal protein VPg, effector of host eukaryotic initiation factor eIF4E.
abstract: Potyvirus RNA contains at the 5' end a covalently linked virus-encoded protein VPg, which is required for virus infectivity. This role has been attributed to VPg interaction with the eukaryotic translation initiation factor eIF4E, a cap-binding protein. We characterized the dissociation constants for the interaction of the potato virus Y VPg with different plant eIF4Es and its isoforms and mapped the eIF(iso)4E attachment region on VPg. VPg/eIF4E interaction results in the inhibition of cell-free protein synthesis, and we show that it stems from the liberation of the cap moiety from the complex with eIF4E. Since VPg does not attach the cap, it appears that VPg induces changes in the eIF4E structure, diminishing its affinity to the cap. We show here that the initiation complex scaffold protein eIF(iso)4G increases VPg interaction with eIF(iso)4E. These data together suggest similar cap and VPg interactions with eIF4E and characterize VPg as a novel eIF4E-binding protein, which inhibits host protein synthesis at a very early stage of the initiation complex formation through the inhibition of cap attachment to the initiation factor eIF4E.
authors.affiliation: Institute of Biochemistry and Biophysics (IBB), Polish Academy of Sciences, Warsaw, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Grzela
authors.forename: L
authors.initials: L
authors.lastname: Strokovska
authors.forename: J-P
authors.initials: JP
authors.lastname: Andrieu
authors.forename: B
authors.initials: B
authors.lastname: Dublet
authors.forename: W
authors.initials: W
authors.lastname: Zagorski
authors.forename: J
authors.initials: J
authors.lastname: Chroboczek
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002846:Chromatography, Affinity; D002918:Chymotrypsin / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D039561:Eukaryotic Initiation Factor-4E / Q000737:chemistry / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D008969:Molecular Sequence Data; D017800:Potyvirus / Q000378:metabolism* / Q000472:pathogenicity; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012315:RNA Caps / Q000378:metabolism; D020539:Sequence Analysis, Protein; D011198:Solanum tuberosum / Q000378:metabolism / Q000821:virology; D014357:Trypsin / Q000378:metabolism; D014758:Viral Core Proteins / Q000737:chemistry / Q000378:metabolism*; D014774:Virulence
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D020033:Protein Isoforms; D012315:RNA Caps; D014758:Viral Core Proteins; D006160:Guanosine Triphosphate; D002918:Chymotrypsin; D014357:Trypsin