title: Cloning and characterization of an RNase-related protein gene preferentially expressed in rice stems.
abstract: RNase-related proteins (RRPs) are S- and S-like RNase homologs lacking the active site required for RNase activity. Here we describe the cloning and characterization of the rice (Oryza sativa) RRP gene (OsRRP). A single copy of OsRRP occurs in the rice genome. OsRRP contains three introns and an open reading frame encoding 252 amino acids, with the replacement of two histidines involved in the active site of RNase by lysine and tyrosine respectively. OsRRP is preferentially expressed in stems of wild-type rice and is significantly down-regulated in an increased tillering dwarf mutant ext37.
authors.affiliation: State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Jun-Ya
authors.initials: JY
authors.lastname: Wei
authors.forename: An-Ming
authors.initials: AM
authors.lastname: Li
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Liu
authors.forename: Liang-Shi
authors.initials: LS
authors.lastname: Liu
authors.forename: Zeng-Fu
authors.initials: ZF
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012260:Ribonucleases / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D012260:Ribonucleases