Adipose aspirates as a source for human processed lipoaspirate cells after optimal cryopreservation.
BACKGROUND
The purpose of this study was to test the authors' hypothesis that previously cryopreserved adipose aspirates collected from conventional liposuction could still be a reliable source of human processed lipoaspirate cells.


METHODS
Adipose aspirates were collected from 12 adult female patients after conventional liposuction of the abdomen and were then preserved by an optimal cryopreservation method with added cryoprotective agents (0.5 M dimethyl sulfoxide and 0.2 M trehalose). Cryopreservation of the adipose tissues was subsequently conducted with controlled slow cooling and then stored in liquid nitrogen (-196 degrees C). One gram of fresh or cryopreserved (after fast rewarming) adipose aspirates was processed in vitro and the resulting cell pellet, consisting of processed lipoaspirate cells, was cultured separately. The length of time until processed lipoaspirate cells became adherent to the culture plate was recorded and the number of processed lipoaspirate cells after a 2-week culture was counted.


RESULTS
Flat, spindle-shape processed lipoaspirate cells from the cryopreserved group became adherent to the plate within 48 to 72 hours after initial culture compared with the fresh group, where the cells became adherent by 24 hours. After a 2-week culture, the cryopreserved aspirates yielded an average of 3.7 +/- 1.4 x 10(5) processed lipoaspirate cells per milliliter, equal to 90 percent of the yielded number of cells obtained from the fresh aspirates (4.1 +/- 1.4 x 10(5) cells/ml).


CONCLUSIONS
The authors' results indicate that although there is a latency of cell growth after an optimal cryopreservation, cryopreserved adipose aspirates can yield a significant number of processed lipoaspirate cells compared with fresh aspirates and may be a reliable source of human processed lipoaspirate cells because they can still be processed later after long-term preservation.
Pu
Lee L Q
LL
Division of Plastic Surgery, Department of Mechanical Engineering, University of Kentucky, Lexington, KY, USA. lpu@uky.edu
Cui
Xiangdong
X
Fink
Betsy F
BF
Gao
Dayong
D
Vasconez
Henry C
HC
D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D000328:Adult; D002455:Cell Division; D002469:Cell Separation / Q000379:methods; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured / Q000166:cytology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D006801:Humans; D015187:Lipectomy; D008875:Middle Aged; D050507:Subcutaneous Fat, Abdominal / Q000166:cytology*; D013396:Suction; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014199:Trehalose / Q000494:pharmacology
D003451:Cryoprotective Agents; D014199:Trehalose; D004121:Dimethyl Sulfoxide