title: Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation.
abstract: Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasmid/PEI complexes in CHO cells.
authors.affiliation: Department of Textile Science, Otsuma Women's University, Tokyo, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ito
authors.forename: Naoko
authors.initials: N
authors.lastname: Iida-Tanaka
authors.forename: Takuro
authors.initials: T
authors.lastname: Niidome
authors.forename: Takahito
authors.initials: T
authors.lastname: Kawano
authors.forename: Koji
authors.initials: K
authors.lastname: Kubo
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sato
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Yang
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Koyama
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D016503:Drug Delivery Systems / Q000379:methods*; D004347:Drug Interactions / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000031:analogs & derivatives*; D008297:Male; D051379:Mice; D015533:Transcriptional Activation / Q000187:drug effects* / Q000502:physiology
chemical_list: D006820:Hyaluronic Acid