Raloxifene promotes adipocyte differentiation of 3T3-L1 cells.
To explore the possibility that raloxifene might influence an adipocyte differentiation and lipogenesis, we studied the effects of raloxifene on the expression of adiponectin and other peroxisome proliferator-activated receptor gamma targeting genes using the 3T3-L1 adipocytes. With standard adipogenic inducers, we added raloxifene at various doses for the adipocyte differentiation. Higher doses of raloxifene facilitated lipid accumulation of the 3T3-L1 cells. We next examined the differentiating and differentiated adipocytes and found that raloxifene augmented mRNA levels of adiponectin, adipocyte-specific fatty acid binding protein, and lipoprotein lipase dose-dependently in both. These effects were opposite those of 17beta-estradiol treatment. These findings suggest that raloxifene promotes adipocyte differentiation, providing a novel insight into the treatment of postmenopausal metabolic syndrome with hypoadiponectinemia.
Murase
Yuko
Y
Department of Molecular Genetics of Cardiovascular Disorders, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8640, Japan.
Kobayashi
Junji
J
Nohara
Atsushi
A
Asano
Akimichi
A
Yamaaki
Naoto
N
Suzuki
Kaoru
K
Sato
Hiroshi
H
Mabuchi
Hiroshi
H
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D052242:Adiponectin / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D011464:Epoprostenol / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis; D008071:Lipoprotein Lipase / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020849:Raloxifene Hydrochloride / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D052242:Adiponectin; D004965:Estrogen Antagonists; D050556:Fatty Acid-Binding Proteins; D008055:Lipids; D012333:RNA, Messenger; D020849:Raloxifene Hydrochloride; D004958:Estradiol; D011464:Epoprostenol; D008071:Lipoprotein Lipase