A slide latex agglutination test for the rapid detection of antibodies in serum against porcine parvovirus.
A slide latex agglutination test (LAT) was developed and evaluated to detect serum antibodies against porcine parvovirus. Porcine parvovirus antigen was obtained by 10% PEG-6000 and 0.5 mol/l sodium chloride precipitation, and inactivated by 0.1% methanal. Two per cent suspensions of latex particles (0.5-0.8 microm) were coated by adding an equal volume of porcine parvovirus antigen at 0.34 microg/ml. Repeatability of latex agglutination test was evaluated with a panel of 100 sera using the same and different antigen lots. A good agreement between LAT and haemagglutination inhibit assay was observed. Because of convenience and speed of performance, this method would be used widely in clinic examination.
Lü
J
J
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Zhao
J
J
Fang
L
L
He
Q
Q
Cao
S
S
Chen
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D007841:Latex Fixation Tests / Q000379:methods / Q000662:veterinary*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D028323:Parvovirus, Porcine / Q000276:immunology*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D013997:Time Factors
D000914:Antibodies, Viral