title: [Isolation and identification of arboviruses in Hebei Province].
abstract: BACKGROUND
  To study the arboviruses carried by mosquitoes collected in Hebei Province.
  
  
  METHODS
  Samples were collected from mosquito active sites and stored in liquid nitrogen till use. Pools of 20 to 30 mosquitoes were ground after sterilization, centrifugal supernant was inoculated onto C6/36 cell, cytopathic effect was observed for three sequential passages. Positive isolates were identified by IFA and RT-PCR.
  
  
  RESULTS
  Totally 1310 mosquitoes were collected from two villages of She county, Hebei province. They were divided into 46 pools and ground respectively. Thirteen positive isolates were obtained. Two isolates reacted with alphaviral antibodies and were amplified by alphaviral primers, nucleotide sequence showed the highest homology (98%) to Getah virus (AY702913.1), so the two isolates were identified as Getah virus.
  
  
  CONCLUSIONS
  Getah virus was isolated from mosquitoes in Hebei Province. This is the first report of isolating Getah virus from inland of China.
authors.affiliation: Institute of Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Huan-qin
authors.initials: HQ
authors.lastname: Wang
authors.forename: Wei-bing
authors.initials: WB
authors.lastname: Liu
authors.forename: Dong-rong
authors.initials: DR
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Jun-wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Lian-shan
authors.initials: LS
authors.lastname: Zhang
authors.forename: Jia-wei
authors.initials: JW
authors.lastname: Liu
authors.forename: San-ju
authors.initials: SJ
authors.lastname: Tao
authors.forename: Xin-jun
authors.initials: XJ
authors.lastname: Lv
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002460:Cell Line; D016000:Cluster Analysis; D009033:Culicidae / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA