title: Different inflammatory response and oxidative stress in neointimal hyperplasia after balloon angioplasty and stent implantation in cholesterol-fed rabbits.
abstract: Inflammatory responses appear to play an important role in the occurrence of restenosis following coronary intervention. However, the contribution of C-reactive protein (CRP) and oxidative stress to restenosis after balloon angioplasty and stent implantation remains unclear. The aim of this study was to examine this issue using hyperlipidemic rabbits. Rabbits were divided into two groups; they were fed with a 0.5% cholesterol diet and with a mixed 0.5% cholesterol and 0.5% probucol diet. Each group of rabbits underwent balloon injury and stent implantation in right and left iliac arteries, respectively. Eight weeks after the intervention, we examined luminal stenosis, neointimal hyperplasia, immunoreactivity for macrophage, CRP and oxidized phosphatidylcholine (oxPC), and also the expression of CRP mRNA. The degrees of neointimal hyperplasia and immunopositive areas (%) for macrophage, CRP, and oxPC in the neointima were significantly higher after stent implantation than after balloon injury, but CRP mRNA was undetectable in either artery. Anti-oxidant probucol reduced angiographic stenosis, neointimal hyperplasia, and macrophage- and oxPC-positive areas much more significantly after stenting. The results demonstrate that the inflammatory response to the development of neointimal hyperplasia differs after balloon injury and stent implantation and that CRP deposition and oxidative stress might be involved more significantly in neointimal development after stent implantation.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692 Japan.
authors.forename: Riichirou
authors.initials: R
authors.lastname: Kawamoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamashita
authors.forename: Kensaku
authors.initials: K
authors.lastname: Nishihira
authors.forename: Eiji
authors.initials: E
authors.lastname: Furukoji
authors.forename: Kinta
authors.initials: K
authors.lastname: Hatakeyama
authors.forename: Tetsunori
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Takuroh
authors.initials: T
authors.lastname: Imamura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Itabe
authors.forename: Tanenao
authors.initials: T
authors.lastname: Eto
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Asada
mesh_terms: D000800:Angioplasty, Balloon / Q000009:adverse effects*; D000818:Animals; D002097:C-Reactive Protein / Q000235:genetics*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004195:Disease Models, Animal; D006083:Graft Occlusion, Vascular / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D006965:Hyperplasia / Q000209:etiology / Q000378:metabolism; D007083:Iliac Artery / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D008055:Lipids / Q000097:blood; D008264:Macrophages / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D010713:Phosphatidylcholines / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D011859:Radiography; D015607:Stents / Q000009:adverse effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D017539:Tunica Intima / Q000293:injuries / Q000378:metabolism*
chemical_list: D002791:Cholesterol, Dietary; D008055:Lipids; D010713:Phosphatidylcholines; D012333:RNA, Messenger; D017392:Thiobarbituric Acid Reactive Substances; D002097:C-Reactive Protein