Select the best: novel biocatalysts for industrial applications.
The number of biocatalysts needed for biotechnological applications is increasing rapidly, necessitating the development of novel identification systems. To date, strategies based on high-throughput screening and selection are applied, with the latter enabling a desired phenotype to survive under appropriate conditions. The groups of Witholt and de Lorenzo have devised a novel selection system, comprising a transcriptional regulator that is activated upon binding to the product of a biocatalytic reaction, which paves the way for easier selection of many different biocatalysts.
Konarzycka-Bessler
Monika
M
Institute of Molecular Enzyme Technology, Heinrich-Heine University Duesseldorf, Research Centre Juelich, D-52428 Juelich, Germany.
Jaeger
Karl-Erich
KE
D001419:Bacteria / Q000201:enzymology*; D015374:Biosensing Techniques / Q000379:methods; D002384:Catalysis; D004789:Enzyme Activation / Q000235:genetics; D004798:Enzymes / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods*; D013379:Substrate Specificity
D004798:Enzymes