Tryptophan Biosynthesis in Cell Cultures of Nicotiana tabacum.
Some of the general features of the pathway for l-tryptophan biosynthesis in cell cultures of Nicotiana tabccum var. Wisc. 38 have been investigated. The results of both isotope competition and direct-labeling experiments show that shikimic acid, anthranilic acid, indoleglycerol phosphate, and indole can serve as precursors to l-tryptophan in these cells, indicating that, in terms of its biochemical intermediates, the pathway is similar to that described for the bacteria and fungi.
Delmer
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Department of Biology, Revelle College, University of California, San Diego, La Jolla, California 92037.
Mills
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SE