title: Use of Griess reagent containing vanadium(III) for post-column derivatization and simultaneous determination of nitrite and nitrate in baby food.
abstract: An ion chromatographic method with post-column derivatization and spectrophotometric detection is presented for the determination of nitrate and nitrite (NOx) in baby food. NOx residues found naturally or added as preservatives were extracted from baby foods and determined by using ion chromatography with post-column derivatization and spectrophotometric detection. Nitrate was reduced to nitrite online by post-column reduction using vanadium(lll) chloride and heat. Nitrite reacted with Griess reagent to produce a dye that was detected at 525 nm. The use of V(III) and heat to promote the reduction of nitrate to nitrite online is a novel feature of this detection system. The determination of incurred NOx residues in samples by using AOAC Method 993.03 yielded results comparable to those obtained by ion chromatography with spectrophotometric detection. The toxic and carcinogenic metal cadmium used in the AOAC Method to reduce the nitrate to nitrite was avoided. The proposed method provides simultaneous determination of nitrate and nitrite. Average recoveries of nitrate and nitrite residues ranged from 82 to 107% for fortification levels of 25-400 ppm.
authors.affiliation: U.S. Food and Drug Administration, Southeast Regional Laboratory, 60 Eighth St NE, Atlanta, GA 30309, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Casanova
authors.forename: Lois K
authors.initials: LK
authors.lastname: Gross
authors.forename: Sarah E
authors.initials: SE
authors.lastname: McMullen
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Schenck
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005029:Ethylenediamines / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D009566:Nitrates / Q000032:analysis*; D009573:Nitrites / Q000032:analysis*; D012015:Reference Standards; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013424:Sulfanilamides / Q000737:chemistry*; D014639:Vanadium / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry
chemical_list: D005029:Ethylenediamines; C095000:Griess reagent; D007202:Indicators and Reagents; D009566:Nitrates; D009573:Nitrites; D012996:Solutions; D013424:Sulfanilamides; D014639:Vanadium