Simultaneous analysis of prostanoids using liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry.
A liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry (LC-FAIMS-MS/MS) semi-quantitative method was developed for the simultaneous determination of prostaglandin (PG) E2, PGD2, PGF(2alpha), 6-keto-PGF(1alpha), and thromboxane (TX) B2. Diluted samples containing these prostanoids and their tetra-deuterium-substituted internal standards were analyzed by LC followed by either selected reaction monitoring (LC-SRM) or FAIMS and selected reaction monitoring (LC-FRM). FAIMS reduced background noise, separated the isobaric ions PGE2 and PGD2, and separated dynamically interchanging TXB2 anomers. This is the first report of the separation of interconverting anomers by FAIMS. Generally, the LC-FRM chromatograms were more selective than the LC-SRM chromatograms. Its potential was demonstrated by analysis of prostanoids in guinea pig lumbar spinal cord homogenate.
Kapron
James
J
Ionalytics, M50-IPF, 1200 Montreal Rd, Ottawa, ON, K1A 0R6 Canada. james.kapron@thermo.com
Wu
Jin
J
Mauriala
Timo
T
Clark
Patsy
P
Purves
Randy W
RW
Bateman
Kevin P
KP
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003903:Deuterium; D006168:Guinea Pigs; D008297:Male; D011453:Prostaglandins / Q000032:analysis*; D012015:Reference Standards; D013116:Spinal Cord / Q000737:chemistry; D053719:Tandem Mass Spectrometry
D011453:Prostaglandins; D003903:Deuterium