title: [Histopathology analysis of microemboli after thrombolysis].
abstract: OBJECTIVE
  To observe the changes in fibrin of microemboli in micro-embolism soon after thrombolysis, in order to provide a theoretical basis for micro-thrombolytic therapy.
  
  
  METHODS
  Thirty adult male SD rats weighing (180+/-10)g were randomized into five groups: 5, 30, 60 and 90 minutes groups after micro-thromboembolization and the control group. Rat cremasteric artery was embolized with photochemical method, and micro-thrombi were produced after thrombolysis by staphylokinase. The levels of tissue plasminogen activator (t-PA) and tissue plasminogen activator inhibitor (PAI) were immunohistochemically determined, and taken as the markers of fibrin dissolution.
  
  
  RESULTS
  Fibrin was observed in pathologic sections as well as fibrinolytic markers t-PA and PAI in immunohistochemical sections. The levels of t-PA were found to be reduced while that of PAI increased gradually with passage of time.
  
  
  CONCLUSIONS
  Fibrin is one of the main constituents of microthrombi soon after thrombolysis. The content of fibrous emboli increases after microembolization with the passage of time. Thrombolysis plays an important role in the treatment of "no reflow phenomenon".
authors.affiliation: Emergency Department, General Hospital of PLA, Beijing 100853, China. zhuhy301@163.com
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.forename: Yu-ying
authors.initials: YY
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiu-mei
authors.initials: XM
authors.lastname: Zhao
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005337:Fibrin / Q000378:metabolism*; D005342:Fibrinolysis; D005343:Fibrinolytic Agents / Q000627:therapeutic use; D008297:Male; D015849:Plasminogen Inactivators / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013923:Thromboembolism / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D015912:Thrombolytic Therapy; D010959:Tissue Plasminogen Activator / Q000378:metabolism
chemical_list: D005343:Fibrinolytic Agents; D015849:Plasminogen Inactivators; D005337:Fibrin; D010959:Tissue Plasminogen Activator