Effects of anti-inflammatory biflavonoid, ginkgetin, on chronic skin inflammation.
Ginkgetin, a biflavonoid from Ginkgo biloba leaves (Ginkgoaceae), was previously demonstrated to inhibit phospholipase A2 and to suppress proinflammatory gene expression such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase. In this study, the effects of ginkgetin were examined on an animal model of chronic skin inflammation and proinflammatory gene expression. When topically applied to ICR mouse ear, ginkgetin (20-80 microg/ear/treatment) inhibited ear edema (22.8-30.5%) and prostaglandin E2 production (30.2-31.1%) induced by multiple treatment of 12-O-tetradecanoylphorbol-13-acetate (TPA) for 7 consecutive days. By histological comparison, ginkgetin was also found to reduce epidermal hyperplasia. The expression of proinflammatory gene, interleukin-1beta, was suppressed by ginkgetin. From the results, it is suggested that ginkgetin may be beneficial against chronic skin inflammatory disorders like atopic dermatitis.
Lim
Hyun
H
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Son
Kun Ho
KH
Chang
Hyeun Wook
HW
Kang
Sam Sik
SS
Kim
Hyun Pyo
HP
D000287:Administration, Topical; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000627:therapeutic use*; D044946:Biflavonoids / Q000008:administration & dosage / Q000627:therapeutic use*; D002908:Chronic Disease; D003872:Dermatitis / Q000188:drug therapy*; D015232:Dinoprostone / Q000096:biosynthesis / Q000235:genetics; D004487:Edema / Q000188:drug therapy / Q000473:pathology; D005419:Flavonoids / Q000008:administration & dosage / Q000627:therapeutic use*; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000473:pathology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D044946:Biflavonoids; D005419:Flavonoids; C077458:ginkgetin; D015232:Dinoprostone