Resampling and deconvolution of linear time-of-flight records for enhanced protein profiling.
We have developed a peak deconvolution strategy that is applicable to the full mass range of a time-of-flight (TOF) spectrum. This strategy involves resampling a spectrum to create a time series that has equal peak widths (in time) across the entire spectrum, and then using the deconvolution filters we have previously described. We use this technique to deconvolve the protein mass spectra for blood serum and cell lysates acquired on three separate TOF instruments. Following deconvolution, we resolve spectral structures consistent with expected events such as multiply charged ions, matrix adducts and post-translational protein modifications. The deconvolution procedure produces a 40% improvement in the resolution and enhanced experimental sensitivity over the full length of the linear TOF record, up to m/z 150 000. This approach is particularly appropriate for automated data analysis and peak detection in dense TOF spectra.
Malyarenko
Dariya I
DI
Departments of Physics and Applied Science, College of William and Mary, Williamsburg, VA 23187-8795, USA. dimaly@wm.edu
Cooke
William E
WE
Tracy
Eugene R
ER
Drake
Richard R
RR
Shin
Susanna
S
Semmes
O John
OJ
Sasinowski
Maciek
M
Manos
Dennis M
DM
D000465:Algorithms; D016632:Apolipoprotein A-I / Q000032:analysis; D001826:Body Fluids / Q000737:chemistry; D002138:Calibration; D003627:Data Interpretation, Statistical; D019277:Entropy; D004847:Epithelial Cells / Q000737:chemistry; D006801:Humans; D017711:Nonlinear Dynamics; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry*; D044967:Serum / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods / Q000706:statistics & numerical data*
D016632:Apolipoprotein A-I; D011506:Proteins