title: Wnt gradient formation requires retromer function in Wnt-producing cells.
abstract: Wnt proteins function as morphogens that can form long-range concentration gradients to pattern developing tissues. Here, we show that the retromer, a multiprotein complex involved in intracellular protein trafficking, is required for long-range signaling of the Caenorhabditis elegans Wnt ortholog EGL-20. The retromer functions in EGL-20-producing cells to allow the formation of an EGL-20 gradient along the anteroposterior axis. This function is evolutionarily conserved, because Wnt target gene expression is also impaired in the absence of the retromer complex in vertebrates. These results demonstrate that the ability of Wnt to regulate long-range patterning events is dependent on a critical and conserved function of the retromer complex within Wnt-producing cells.
authors.affiliation: Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands.
authors.forename: Damien Y M
authors.initials: DY
authors.lastname: Coudreuse
authors.forename: Giulietta
authors.initials: G
authors.lastname: Roël
authors.forename: Marco C
authors.initials: MC
authors.lastname: Betist
authors.forename: Olivier
authors.initials: O
authors.lastname: Destrée
authors.forename: Hendrik C
authors.initials: HC
authors.lastname: Korswagen
mesh_terms: D000818:Animals; D019521:Body Patterning; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D015870:Gene Expression; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D046912:Multiprotein Complexes / Q000502:physiology*; D009154:Mutation; D009474:Neurons / Q000166:cytology / Q000502:physiology; D034622:RNA Interference; D015398:Signal Transduction; D019076:Transgenes; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000502:physiology; D051153:Wnt Proteins / Q000502:physiology*; D014981:Xenopus
chemical_list: D029742:Caenorhabditis elegans Proteins; C117259:Egl-20 protein, C elegans; D006023:Glycoproteins; D046912:Multiprotein Complexes; C419573:VPS35 protein, human; D033921:Vesicular Transport Proteins; D051153:Wnt Proteins