Determination of glutathione in hemolysed erythrocyte with amperometric sensor based on TTF-TCNQ.
BACKGROUND
GSH has a relevant role in human metabolism as an indicator of disease risks. An amperometric sensor for glutathione (GSH) determination is described as an alternative method featuring simple construction procedure and short time analysis.


METHODS
The developed sensor was used to determine glutathione at low potential using a TTF-TCNQ complex.


RESULTS
The sensor exhibits a linear response range from 5 to 340 micromol/l under applied potential of 200 mV vs. SCE. The sensitivity and detection limit were 90.1 microA l/mmol cm(2) and 0.3 micromol/l, respectively.


CONCLUSIONS
When the sensor was tested in hemolysed erythrocyte samples for GSH determination, a good correlation in results was observed between the sensor and the spectrophotometric method. The sensor showed recovery values between 98% and 102%.
Calvo-Marzal
Percy
P
Instituto de Química-UNICAMP, Departamento de Química Analítica. P.O. Box 6154, 13083-970 Campinas-SP, Brazil.
Chumbimuni-Torres
Karin Y
KY
Höehr
Nelci Fenalti
NF
Kubota
Lauro T
LT
D015374:Biosensing Techniques; D004563:Electrochemistry; D004912:Erythrocytes / Q000737:chemistry*; D005978:Glutathione / Q000097:blood*; D006461:Hemolysis; D006571:Heterocyclic Compounds; D006801:Humans; D007202:Indicators and Reagents; D009570:Nitriles; D015203:Reproducibility of Results; D013053:Spectrophotometry
D006571:Heterocyclic Compounds; D007202:Indicators and Reagents; D009570:Nitriles; C013703:tetracyanoquinodimethane; D005978:Glutathione; C063887:tetrathiafulvalene