title: Neurogenic pain and steroid synthesis in the spinal cord.
abstract: The spinal cord (SC) is a biosynthetic center for neurosteroids, including pregnenolone (PREG), progesterone (PROG), and 3alpha/5alpha-tetrahydroprogesterone (3alpha/5alpha-THP). In particular, an active form of cytochrome P450 sidechain cleavage (P450scc) has been localized in sensory networks of the rat SC dorsal horn (DH). P450scc is the key enzyme catalyzing the conversion of cholesterol (CHOL) into PREG, the rate-limiting step in the biosynthesis of all classes of steroids. To determine whether neurosteroidogenesis might be involved in the pivotal role played by the DH in nociception, effects of neurogenic pain provoked by sciatic nerve ligature were investigated on P450scc expression, cellular distribution, and activity in the SC. P450scc mRNA concentration was threefold higher in the DH of neuropathic rats than in controls. The nerve ligature also increased the density of P450sccpositive neuronal perykarya and fibers in the ipsilateral DH. Incubation of spinal tissue homogenates with [3H]CHOL revealed that the amount of newly synthesized [3H]PREG from [3H]CHOLwas 80% higher in the DH of neuropathic rats. Radioimmunoassays showed an increase of PREG and 3alpha/5alpha-THP concentrations in neuropathic rat DH. The upregulation of PREG and 3alpha/5alpha-THP biosynthesis might be involved in endogenous mechanisms triggered by neuropathic rats to cope with the chronic pain state. 3alpha/5alpha-THP formation from PREG can also generate PROG, which decreases sensitivity to pain and protects nerve cells against degeneration. Because apoptotic cell death has been demonstrated in the DH during neuropathic pain, activation of neurosteroidogenesis in spinal tissues might also be correlated to the neuroprotective role of steroids in the SC.
authors.affiliation: Institut des Neurosciences Cellulaires et Intégratives-Centre National de la Recherche Scientifique, Université Louis Pasteur, 67084 Strasbourg Cedex, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Patte-Mensah
authors.forename: Cherkaouia
authors.initials: C
authors.lastname: Kibaly
authors.forename: Domitille
authors.initials: D
authors.lastname: Boudard
authors.forename: Véronique
authors.initials: V
authors.lastname: Schaeffer
authors.forename: Aurélie
authors.initials: A
authors.lastname: Béglé
authors.forename: Simona
authors.initials: S
authors.lastname: Saredi
authors.forename: Laurence
authors.initials: L
authors.lastname: Meyer
authors.forename: Ayikoe G
authors.initials: AG
authors.lastname: Mensah-Nyagan
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) / Q000378:metabolism; D000818:Animals; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D006801:Humans; D008026:Ligation; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010146:Pain / Q000378:metabolism*; D011284:Pregnenolone / Q000378:metabolism; D011374:Progesterone / Q000031:analogs & derivatives / Q000378:metabolism; D012584:Sciatic Nerve / Q000601:surgery; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis*
chemical_list: D013256:Steroids; D011374:Progesterone; D011284:Pregnenolone; D015096:3-Hydroxysteroid Dehydrogenases; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); D013254:Steroid 17-alpha-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase