Proteomic analysis of prodigiosin-induced apoptosis in a breast cancer mitoxantrone-resistant (MCF-7 MR) cell line.
Prodigiosin (PG) is a bacterial, red-pigmented antibiotic with immunosuppressive and apoptotic activities. To better understand its mechanisms of action, we tried to identify proteins associated with apoptosis induced by PG. For this purpose, the variation of protein expression on exposure to apoptotic concentrations of PG was examined, by high-resolution two-dimensional gel electrophoresis (2D-E), in the MCF-7 cancer cell line resistant to mitoxantrone (MCF-7-MR). Six PG apoptosis-associated protein spots were further characterized by complementary peptide mass fingerprinting and tandem mass spectrometry data obtained on a matrix-assisted laser desorption ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometer. The proteins identified were involved in various cellular functions, including cell defence, DNA repair and cellular organization. Our data provide novel information on cell response to PG, a new apoptotic drug with interesting anticancer activity.
Monge
Marta
M
Department of Pathology and Experimental Therapeutic, Cancer Cell Biology Research Group, University of Barcelona, Barcelona, Spain.
Vilaseca
Marta
M
Soto-Cerrato
Vanessa
V
Montaner
Beatriz
B
Giralt
Ernest
E
Pérez-Tomás
Ricardo
R
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western / Q000379:methods; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005982:Glutathione Transferase / Q000032:analysis; D006801:Humans; D053538:Keratin-18 / Q000032:analysis; D053539:Keratin-19 / Q000032:analysis; D053533:Keratin-8 / Q000032:analysis; D008942:Mitoxantrone / Q000494:pharmacology*; D010750:Phosphoproteins / Q000032:analysis; D011353:Prodigiosin / Q000302:isolation & purification / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D012269:Ribosomal Proteins / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D053538:Keratin-18; D053539:Keratin-19; D053533:Keratin-8; D010750:Phosphoproteins; D020543:Proteome; D012269:Ribosomal Proteins; C054675:ribosomal protein P0; D008942:Mitoxantrone; C483009:GSTM3 protein, human; D005982:Glutathione Transferase; D011353:Prodigiosin