title: p16INK4a overexpression independent of human papillomavirus infection in lobular endocervical glandular hyperplasia.
abstract: A high rate of human papillomavirus (HPV) infection has been reported in cervical cancer and precancerous lesions. Many studies also have shown that p16INK4a overexpression is of diagnostic value for high-risk HPV-related cervical cancer and precursors. Lobular endocervical glandular hyperplasia (LEGH) is a rare lesion of the uterine cervix. There is one report about HPV infection and few studies on p16INK4a expression in LEGH. Therefore, we 1) detected HPV infection and examined p16INK4a expression and 2) observed the relationship between HPV and p16INK4a overexpression in LEGH. The immunohistochemical expression of p16INK4a was studied in 24 cases of LEGH. HPV DNA was also evaluated in these cases using a polymerase chain reaction technique. Strong (++) p16INK4a immunoreactivity was observed in 10 (41.7%) of the 24 LEGH cases; a moderate (+) pattern was observed in 9 (37.5%) cases; a weak (+) pattern was observed in 2 (8.3%) cases; and the remaining 3 (12.5%) cases showed negative expression. Overall, p16INK4a overexpression was seen in 87.5% of the cases (21/24). HPV DNA was not detected in any of the 24 LEGH cases. These results suggest that p16INK4a overexpression is independent of HPV infection in LEGH.
authors.affiliation: Department of Obstetrics and Gynecology, and Clinical Laboratory, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan. ahashi@res.yamanashi-med.ac.jp
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hashi
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Xu
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kondo
authors.forename: Keiko
authors.initials: K
authors.lastname: Hashi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Yuminamochi
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nara
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Murata
authors.forename: Ryohei
authors.initials: R
authors.lastname: Katoh
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hoshi
mesh_terms: D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000737:chemistry* / Q000473:pathology*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000737:chemistry / Q000473:pathology
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral