Induction of hypothalamic serum- and glucocorticoid-induced protein kinase-1 gene expression and its relation to plasma des-acyl ghrelin in energy homeostasis in mice.
Serum- and glucocorticoid-induced protein kinase (SGK) is a serine/threonine-specific protein kinase that is transcriptionally regulated by serum, glucocorticoids, and mineral corticoids. Here, we report that fasting or obesity with hyperphagia increased hypothalamic SGK-1 gene expression. Hypothalamic SGK-1 mRNA levels were proportional to daily food intake and body weights in C57BL6J mice, KK mice, and KKA(y) mice matched for age. Plasma des-acyl ghrelin, but not active ghrelin, levels were inversely proportional to daily food intake and body weights among these animals. The increases in hypothalamic SGK-1 gene expression in these animals were not accompanied by increases in plasma corticosterone levels. Under conditions of increased energy usage such as fasting, hypothalamic SGK-1 gene expression and plasma des-acyl ghrelin levels were positively correlated while during conditions of increased energy storage, they were negatively correlated. These results suggest that hypothalamic SGK-1 gene is a novel candidate gene involving in energy homeostasis in mice.
Nonogaki
Katsunori
K
Center of Excellence, Division of Molecular Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Miyagi 980-8575, Japan. knonogaki-tky@umin.ac.jp
Ohashi-Nozue
Kana
K
Oka
Yoshitomo
Y
D000818:Animals; D004734:Energy Metabolism / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D054439:Ghrelin; D006706:Homeostasis / Q000502:physiology*; D007031:Hypothalamus / Q000502:physiology*; D017874:Immediate-Early Proteins / Q000378:metabolism*; D051379:Mice; D036361:Peptide Hormones / Q000097:blood*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D000099184:Serum-Glucocorticoid Regulated Kinases
D054439:Ghrelin; D017874:Immediate-Early Proteins; D036361:Peptide Hormones; D017346:Protein Serine-Threonine Kinases; D000099184:Serum-Glucocorticoid Regulated Kinases; C422650:ghrelin, des-n-octanoyl