title: Dynein light chain Tctex-1 identifies neural progenitors in adult brain.
abstract: The identity and biology of stem cells and progenitors in the adult brain are of considerable interest, because these cells hold great promise for the development of novel therapies for damaged brain tissue in human diseases. This research field critically needs biological markers that specifically identify the resident precursors in the germinal zones of the adult central nervous system so that the discovery of regulatory influences for adult neurogenesis may be facilitated. In this study, by using a combination of in situ hybridization, bromodeoxyuridine incorporation, immunocolocalization, and ultrastructural studies, we show that in rodents Tctex-1, a cytoplasmic dynein light chain, is selectively enriched in almost all cycling progenitors and young neuronal progeny, but not in mature granular cells and astrocytes, in the subgranular zone of the adult dentate gyrus. Tctex-1 is also selectively abundant in cells closely resembling previously described immature progenitors and migrating neuroblasts at the subventricular zone of the lateral ventricle. Our results suggest that Tctex-1 serves as a novel marker for the identification of neural progenitors of the adult brain.
authors.affiliation: Graduate Program in Neuroscience, Weill Medical College of Cornell University, New York, NY 10021, USA.
authors.forename: Carlos
authors.initials: C
authors.lastname: Dedesma
authors.forename: Jen-Zen
authors.initials: JZ
authors.lastname: Chuang
authors.forename: Peter D
authors.initials: PD
authors.lastname: Alfinito
authors.forename: Ching-Hwa
authors.initials: CH
authors.lastname: Sung
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D001973:Bromodeoxyuridine; D018891:Dentate Gyrus / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D000089622:Doublecortin Domain Proteins; D004398:Dyneins; D005260:Female; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000096:biosynthesis; D009687:Nuclear Proteins / Q000096:biosynthesis*; D016601:RNA-Binding Proteins / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000378:metabolism*; D056427:t-Complex Genome Region
chemical_list: D015415:Biomarkers; D000089622:Doublecortin Domain Proteins; C533388:Dynlt1 protein, rat; C533386:Dynlt1b protein, mouse; D008869:Microtubule-Associated Proteins; C491296:Msi1 protein, rat; C104786:Msi1h protein, mouse; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D004398:Dyneins; D001973:Bromodeoxyuridine