title: Expression profiling of radiation-induced genes in radiodermatitis of hairless mice.
abstract: BACKGROUND
  Radiation induces many cellular events leading to radiodermatitis.
  
  
  OBJECTIVE
  The aim of this study was to establish a radiodermatitis model using experimental animals, and to examine the expression profile of radiation-induced genes.
  
  
  METHODS
  Hairless mice were irradiated on the dorsal skin; then total RNAs were isolated and microarray hybridizations were performed.
  
  
  RESULTS
  Irradiation with a total of 40 Gy (10 Gy day-1 for four consecutive days) provokes radiodermatitis in the hairless mouse. After microarray analysis, 130 genes that showed upregulation by radiation were selected and organized into four different clusters, depending on the time-kinetic pattern. Classification of these genes into several functional categories revealed that various biological processes were globally affected by radiation. These include transcription regulation, signal transduction, cell communication, cell death regulation and metabolism.
  
  
  CONCLUSIONS
  These results demonstrate the complexity of the transcriptional profile of the radiation response, providing important clues on which to base further investigations of the molecular events underlying radiodermatitis.
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, Dajeon, and Department of Radiation Oncology, Chosun University Hospital, Gwangju, Korea.
authors.forename: Y S
authors.initials: YS
authors.lastname: Lee
authors.forename: D K
authors.initials: DK
authors.lastname: Choi
authors.forename: C D
authors.initials: CD
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Im
authors.forename: M L
authors.initials: ML
authors.lastname: Mollah
authors.forename: J Y
authors.initials: JY
authors.lastname: Jang
authors.forename: T J
authors.initials: TJ
authors.lastname: Oh
authors.forename: S
authors.initials: S
authors.lastname: An
authors.forename: Y J
authors.initials: YJ
authors.lastname: Seo
authors.forename: G M
authors.initials: GM
authors.lastname: Hur
authors.forename: M J
authors.initials: MJ
authors.lastname: Cho
authors.forename: J K
authors.initials: JK
authors.lastname: Park
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D046228:Microarray Analysis; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods; D011855:Radiodermatitis / Q000209:etiology / Q000235:genetics*; D015854:Up-Regulation / Q000528:radiation effects; D015431:Weight Loss / Q000528:radiation effects