Effects of heat shock protein gp96 on human dendritic cell maturation and CTL expansion.
We reported previously that heat shock protein gp96 and its N-terminal fragment were able to stimulate CTL expansion specific for a HBV peptide (SYVNTNMGL) in BALB/c mice. Here we characterized the adjuvant effects of gp96 on human HLA-A2 restricted T cells. Full-length gp96 isolated from healthy human liver and recombinant fragments both from prokaryotic cells and eukaryotic cells were analyzed for their ability to stimulate maturation of human dendritic cells. It was found that in vitro these proteins were capable of maturating human monocyte-derived dendritic cells (MDDC) isolated from healthy donors as well as from HBV-positive, hepatocellular carcinoma (HCC) patients. In HLA-A2.1/Kb transgenic mice, gp96 and the recombinant fragments were found to augment CTL response specific for the HBcAg(18-27) FLPSDFFPSV peptide of hepatitis B virus.
Zhang
Yuxia
Y
Molecular Virology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Zan
Yanlu
Y
Shan
Ming
M
Liu
Changmei
C
Shi
Ming
M
Li
Wei
W
Zhang
Zhixin
Z
Liu
Na
N
Wang
Fusheng
F
Zhong
Weidong
W
Liao
Fulian
F
Gao
George F
GF
Tien
Po
P
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000473:pathology*; D006360:Heat-Shock Proteins / Q000008:administration & dosage; D006509:Hepatitis B / Q000473:pathology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000473:pathology*
D006360:Heat-Shock Proteins; D008562:Membrane Glycoproteins; C053387:endoplasmin