[Isolation and identification of arboviruses in Hebei Province].
BACKGROUND
To study the arboviruses carried by mosquitoes collected in Hebei Province.


METHODS
Samples were collected from mosquito active sites and stored in liquid nitrogen till use. Pools of 20 to 30 mosquitoes were ground after sterilization, centrifugal supernant was inoculated onto C6/36 cell, cytopathic effect was observed for three sequential passages. Positive isolates were identified by IFA and RT-PCR.


RESULTS
Totally 1310 mosquitoes were collected from two villages of She county, Hebei province. They were divided into 46 pools and ground respectively. Thirteen positive isolates were obtained. Two isolates reacted with alphaviral antibodies and were amplified by alphaviral primers, nucleotide sequence showed the highest homology (98%) to Getah virus (AY702913.1), so the two isolates were identified as Getah virus.


CONCLUSIONS
Getah virus was isolated from mosquitoes in Hebei Province. This is the first report of isolating Getah virus from inland of China.
Wang
Huan-qin
HQ
Institute of Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.
Liu
Wei-bing
WB
Yang
Dong-rong
DR
Liang
Yong
Y
Wang
Jun-wei
JW
Zhang
Lian-shan
LS
Liu
Jia-wei
JW
Tao
San-ju
SJ
Lv
Xin-jun
XJ
Liang
Guo-dong
GD
D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002460:Cell Line; D016000:Cluster Analysis; D009033:Culicidae / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA