title: [The effects of Th1/Th2 cells on in vitro expansion and colony forming of CD34+ cells of aplastic anemic patient].
abstract: OBJECTIVE
  To investigate the effects of both Th1/Th2 imbalance and its re-attainment on in vitro expansion and hematopoiesis of CD34+ cells from a severe aplastic anemia (sAA) patient.
  
  
  METHODS
  A preliminary-diagnosed sAA patient was studied. (1) Bone marrow mononuclear cells (BMMNC) were isolated and CD34+ and CD4+ cells were enriched by magnetic beads respectively. (2) The Th1/Th2 cell ratio within CD4+ subset was detected by flow cytometry (FCM). (3) Enriched-CD34+ cells were expanded and re-enriched for enough cells to be divided into four groups: control, Th cell treatment, Th cell and IFN-gamma treatment, and Th cell and IL-4 treatment, respectively. (4) After expansion for 10 d, colony-forming unit assay was performed on cells in each group. (5) Patient's Th1/Th2 ratio was followed up by FCM after immunotherapy.
  
  
  RESULTS
  (1) Symptom remission was achieved after therapy for 5 months. (2) Th1/Th2 cell ratio of the patient before and after remission was 22.47 and 12.27, respectively, while that of healthy controls was 8.98+/-4.45. (3) The CD34+ cell expansion rates as well as CFU numbers, from high to low, were ranked as control, Th cell and IL-4 contained group, Th cell contained, and Th cell and IFN-gamma contained group.
  
  
  CONCLUSIONS
  Predominant Th1 cells seem to directly inhibit the self-renewal, proliferation and lineage differentiation of CD34+ cells in vitro, which can be counteracted by IL-4, probably mediated by switching Th1/Th2 balance.
authors.affiliation: Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. zhtsmy@fmmu.edu.cn
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Bing-zhong
authors.initials: BZ
authors.lastname: Sun
authors.forename: Xie-qun
authors.initials: XQ
authors.lastname: Chen
authors.forename: Hua-feng
authors.initials: HF
authors.lastname: Zhu
authors.forename: Qing-da
authors.initials: QD
authors.lastname: Qiao
mesh_terms: D000741:Anemia, Aplastic / Q000276:immunology* / Q000473:pathology*; D018952:Antigens, CD34 / Q000276:immunology*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D008297:Male; D016176:T-Lymphocyte Subsets; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D018952:Antigens, CD34; D015847:Interleukin-4; D007371:Interferon-gamma