A dominant-negative effect of cynomolgus monkey tripartite motif protein TRIM5alpha on anti-simian immunodeficiency virus SIVmac activity of an African green monkey orthologue.
African green monkey (AGM) tripartite motif protein (TRIM) 5alpha can inhibit both human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus SIVmac, whereas cynomolgus monkey (CM) TRIM5alpha can inhibit HIV-1, but not SIVmac. We previously reported that the 17-amino-acid region and an adjacent 20-amino-acid duplication in the SPRY(B30.2) domain of AGM TRIM5alpha determined the species specificity. In the present study, we demonstrated that CM TRIM5alpha had a dominant-negative effect on the anti-SIVmac activity of AGM TRIM5alpha. In contrast, mutant TRIM5alphas lacking the 20-amino-acid duplication did not have the dominant-negative effect, even though they failed to restrict SIVmac. These results indicated that oligomerization of the SPRY domain is required for anti-SIVmac activity and suggest that tight interaction between the viral capsid and all three molecules in one TRIM5alpha trimer may not be necessary for restriction activity.
Nakayama
Emi E
EE
Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3-1, Yamada-oka, Suita-shi, Osaka 565-0871, Japan. emien@biken.osaka-u.ac.jp
Maegawa
Hikoichiro
H
Shioda
Tatsuo
T
D020816:Amino Acid Motifs; D000818:Animals; D000998:Antiviral Agents / Q000276:immunology; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002522:Chlorocebus aethiops / Q000235:genetics / Q000276:immunology*; D005799:Genes, Dominant / Q000235:genetics*; D008252:Macaca fascicularis; D009154:Mutation / Q000235:genetics*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*
D000998:Antiviral Agents; D002352:Carrier Proteins