Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3-.
Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO3-. Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO3- secretion, whose level correlates with the severity of diarrhea.
Takahashi
Akira
A
Department of Nutrition and Metabolism, The University of Tokushima Graduate School, Institute of Health Biosciences, Tokushima City, Tokushima, Japan. akiratak@nutr.med.tokushima-u.ac.jp
Nakano
Masayuki
M
Okamoto
Keinosuke
K
Fujii
Yoshio
Y
Mawatari
Kazuaki
K
Harada
Nagakatsu
N
Nakaya
Yutaka
Y
D000333:Aeromonas / Q000472:pathogenicity*; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism*; D018938:Caco-2 Cells; D002712:Chlorides / Q000378:metabolism; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000502:physiology; D003967:Diarrhea / Q000209:etiology*; D006460:Hemolysin Proteins / Q000633:toxicity*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D051379:Mice
D001639:Bicarbonates; D002712:Chlorides; D006460:Hemolysin Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator