title: The use of newly developed real-time PCR for the rapid identification of bacteria in culture-negative osteomyelitis.
abstract: We report a case of a culture-negative osteomyelitis in which our newly developed real-time polymerase chain reaction (PCR) could differentiate Staphylococcus aureus from Staphylococcus epidermidis. This is the first report that described the application of this novel assay to an orthopedics clinical sample. This assay may be useful for other clinical culture-negative cases in a combination with a broad-spectrum assay as a rapid microorganism identification method.
authors.affiliation: Department of Anatomic Pathology L25, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
authors.forename: Naomi
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Bauer
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Sakai
authors.forename: Daisuke
authors.initials: D
authors.lastname: Togawa
authors.forename: Isador H
authors.initials: IH
authors.lastname: Lieberman
authors.forename: Takaaki
authors.initials: T
authors.lastname: Fujishiro
authors.forename: Gary W
authors.initials: GW
authors.lastname: Procop
mesh_terms: D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D010019:Osteomyelitis / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000235:genetics; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial