Development and biological properties of a new live attenuated mumps vaccine.
To develop a new live attenuated mumps vaccine, a wild mumps Y7 strain isolated from a patient who developed mild parotitis was treated with nitrosoguanidine and ultraviolet, followed by selection of a temperature-sensitive clone. The selected clone, Y125, showed stable temperature-sensitivity in Vero cells. Intraspinal inoculation of marmosets with the Y125 produced only minimal histopathological changes, while intracerebral inoculation of neonatal rats revealed that the Y125 did not cause hydrocephalus. Both these effects of the Y125 were similar to those of the non-neurovirulent Jeryl Lynn strain. Furthermore, subcutaneous inoculation of the Y125 induced high levels of neutralizing antibodies in all Cercopithecus monkeys examined. Although the safety and immunogenicity should be confirmed in further field trials in humans, the present results indicate that the Y125 could be a promising vaccine candidate.
Saika
Shizuko
S
Chiba Prefectural Institute of Public Health, 666-2 Nitona-Cho, Chuo-Ku, Chiba-City, Chiba 260-8715, Japan. s.sik@ma.pref.chiba.jp
Kidokoro
Minoru
M
Kubonoya
Hiroko
H
Ito
Kozo
K
Ohkawa
Tokitada
T
Aoki
Athuko
A
Nagata
Noriyo
N
Suzuki
Kazuyoshi
K
D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D001921:Brain / Q000821:virology; D002144:Callithrix; D002522:Chlorocebus aethiops; D005260:Female; D006651:Histocytochemistry; D009107:Mumps / Q000276:immunology* / Q000517:prevention & control; D009108:Mumps Vaccine / Q000235:genetics / Q000276:immunology*; D009109:Mumps virus / Q000235:genetics / Q000276:immunology*; D016296:Mutagenesis; D009500:Neutralization Tests; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014709:Vero Cells
D000914:Antibodies, Viral; D009108:Mumps Vaccine; D012367:RNA, Viral; D014613:Vaccines, Attenuated