Interference of enalaprilat on glomerular permeability to macromolecules (IgG) in acute unilateral ureteral obstruction in rats.
BACKGROUND
Unilateral ureteral obstruction breaks out events that cause the transitory increase of glomerular permeability to macromolecules, both in the obstructed kidney and in the contralateral kidney, suggesting the presence of some factor, with a systemic action, liberated as a response to the obstruction. We know that the rennin-angiotensin system is activated by acute ureteral obstruction. We have developed an experiment to assess the role of angiotensin II on the glomerular permeability to IgG due to acute ureteral obstruction, using enalaprilat, an angiotensin enzyme conversion inhibitor, to block the effects of the activation of the rennin-angiotensin system.


METHODS
We have used 45 adult Wistar female rats, distributed into 3 main groups: a control group with 5 animals and 2 experiment groups each one with 10 animals submitted to unilateral ureteral obstruction and nephrectomy at 60 and 120 minutes. Each experiment group had its simulation correspondent (sham). We have studied both kidneys through the direct immunofluorescence method.


RESULTS
We have found positive permeation in animals without enalaprilat in both kidneys and negative permeation in those in which the drug was used.


CONCLUSIONS
We have concluded that enalaprilat interferes in this alteration of permeability, suggesting that angiotensin II is involved in the loss of selectivity of the glomerular membrane.
Oliveira
Rene M
RM
Section of Urology, Clementino Fraga Filho University Hospital, UFRJ, Rio de Janeiro, RJ, Brazil. renemurilo@aol.com
Oliveira Neto
Antonio A
AA
Miranda
Luiz C
LC
D000208:Acute Disease; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D004195:Disease Models, Animal; D015773:Enalaprilat / Q000494:pharmacology*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D005919:Glomerular Filtration Rate / Q000187:drug effects*; D007074:Immunoglobulin G / Q000378:metabolism*; D046911:Macromolecular Substances / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014517:Ureteral Obstruction / Q000378:metabolism*
D000806:Angiotensin-Converting Enzyme Inhibitors; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D015773:Enalaprilat