[Isolation and identification of Japanese encephalitis virus in Liaoning Province].
BACKGROUND
To study arboviruses carried by mosquitoes in Liaoning Province in 2002.


METHODS
Totally 4927 mosquitoes were collected from Liaoning Province in July 2002. Virus strains were isolated by inoculating BHK-21, C6/36 and Vero cells. The newly isolated strains were identified by serological (ELISA and IFA) and molecular methods (Real-Time PCR and RT-PCR).


RESULTS
Two strains were isolated from mosquitoes causing cytopathogenic effect (CPE) on cells and were fatal to suckling mice. Serological tests showed that both were positive for the antibody to JEV. The PrM and E gene were cloned and sequenced. The phylogenetic analysis showed that the new isolates belonged to genotype I, JEV. Sequence analysis showed that the homology of nucleotide sequences and amino acid (AA) sequences between the two strains was 100%. Compared with the nucleotide sequences between the two strains and the standard JEV vaccine strain SA14-14-2, the difference was up to 4.11%, and the difference of AA was 0.6%.


CONCLUSIONS
Two strains of JEV were isolated and identified in Liaoning province, both belonged to genotype I JEV.
Wang
Jun-wen
JW
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Fu
Shi-hong
SH
Wang
Huan-yu
HY
Mao
Xiao-yan
XY
Liu
Wei-bin
WB
He
Ying
Y
Sun
Xiao-hong
XH
Cai
Zeng-lin
ZL
Wu
Li-ping
LP
Zhao
Xi-fang
XF
Han
Rui-hong
RH
Jing
Ya
Y
Liang
Guo-dong
GD
D000818:Animals; D002460:Cell Line; D002681:China; D002522:Chlorocebus aethiops; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics
D014759:Viral Envelope Proteins; C074932:prM protein, Flavivirus