Inhibition of intestinal carcinogenesis by a new flavone derivative, chafuroside, in oolong tea.
A new flavone derivative, chafuroside, has been isolated as a strong anti-inflammatory compound from oolong tea leaves, and its structure determined to be (2R,3S,4S,4aS,11bS)-3,4,11-trihydroxy-2-(hydroxymethyl)-8-(4-hydroxyphenyl)-3,4,4a,11b-tetrahydro-2H,10H-pyrano[2',3':4,5]furo[3,2-g]chromen-10-one. To assess its potential to inhibit intestinal carcinogenesis, 2.5, 5 and 10 p.p.m. chafuroside was given in the diet to Apc-deficient Min mice for 14 weeks from 6 weeks of age. Total numbers of polyps were reduced to 83, 73 and 56% of the control value, respectively. Moreover, dietary administration at 10 and 20 p.p.m. reduced azoxymethane (AOM)-induced colon aberrant crypt foci (ACF) development in rats to 69% of the AOM-treated control value with the higher dose. Chafuroside-associated toxicity was not observed at 2.5-10 p.p.m. in Min mice and 10-20 p.p.m. in AOM-treated rats. These results suggest that chafuroside might be a good chemopreventive agent for colon cancer.
Niho
Naoko
N
Cancer Prevention Basic Research Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan. nniho@gan2.ncc.go.jp
Mutoh
Michihiro
M
Sakano
Katsuhisa
K
Takahashi
Mami
M
Hirano
Sachiko
S
Nukaya
Haruo
H
Sugimura
Takashi
T
Wakabayashi
Keiji
K
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001397:Azoxymethane / Q000633:toxicity; D028244:Camellia / Q000737:chemistry*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D004032:Diet; D004195:Disease Models, Animal; D005260:Female; D047309:Flavones / Q000627:therapeutic use*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000627:therapeutic use*; D007413:Intestinal Mucosa / Q000187:drug effects; D007417:Intestinal Polyps / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008517:Phytotherapy; D051381:Rats; D011916:Rats, Inbred F344; D013662:Tea
C510948:3,4,11-trihydroxy-2-(hydroxymethyl)-8-(4-hydroxyphenyl)-3,4,4a,11b-tetrahydro-2H,10H-pyrano(2',3'-4,5)furo(3,2-g)chromen-10-one; D000970:Antineoplastic Agents; D047309:Flavones; D006576:Heterocyclic Compounds, 4 or More Rings; D013662:Tea; D001397:Azoxymethane