title: The major subtypes of human B-cell lymphomas lack mutations in BCL-2 family member BAD.
abstract: Members of the BCL-2 gene family are well known for their role in the pathogenesis of B-cell lymphomas in humans and in mouse models. A recent report that knockout mice deficient for the proapoptotic BCL-2 family member gene BAD frequently develop B-cell lymphomas prompted us to analyze a large collection of human B-cell lymphomas for inactivating mutations in the BAD gene. All 3 exons of the BAD gene were amplified and directly sequenced. The 81 lymphomas analyzed included 16 cases of B-cell chronic lymphocytic leukemia, 11 mantle-cell lymphomas, 10 follicular lymphomas, 7 MALT lymphomas, 8 Burkitt's lymphoma cell lines, 3 cell lines of multiple myeloma, 15 cases and 4 cell lines of diffuse large B-cell lymphoma and 7 Hodgkin's lymphoma lines. No mutations were found in any of the cases. We conclude that mutations in the BAD gene do not play a role in the pathogenesis of the major subtypes of human B-cell lymphomas.
authors.affiliation: Institute for Cell Biology (Tumor Research), University of Duisburg-Essen, Medical School, Essen, Germany. roland.schmitz@uni-essen.de
authors.forename: Roland
authors.initials: R
authors.lastname: Schmitz
authors.forename: Roman K
authors.initials: RK
authors.lastname: Thomas
authors.forename: Anne C
authors.initials: AC
authors.lastname: Harttrampf
authors.forename: Claudia
authors.initials: C
authors.lastname: Wickenhauser
authors.forename: Joachim L
authors.initials: JL
authors.lastname: Schultze
authors.forename: Martin-Leo
authors.initials: ML
authors.lastname: Hansmann
authors.forename: Jürgen
authors.initials: J
authors.lastname: Wolf
authors.forename: Ralf
authors.initials: R
authors.lastname: Küppers
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D016393:Lymphoma, B-Cell / Q000145:classification* / Q000235:genetics* / Q000473:pathology; D009154:Mutation / Q000235:genetics; D051025:bcl-Associated Death Protein / Q000235:genetics*
chemical_list: D051025:bcl-Associated Death Protein