title: Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation.
abstract: Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins.
authors.affiliation: Department of Biochemistry, Faculty of Science, Palacký University, Slechtitelů 11, 783 71 Olomouc, Czech Republic. sebela@prfholnt.upol.cz
authors.forename: Marek
authors.initials: M
authors.lastname: Sebela
authors.forename: Tat'ána
authors.initials: T
authors.lastname: Stosová
authors.forename: Jan
authors.initials: J
authors.lastname: Havlis
authors.forename: Natalie
authors.initials: N
authors.lastname: Wielsch
authors.forename: Henrik
authors.initials: H
authors.lastname: Thomas
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Zdráhal
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
mesh_terms: D000818:Animals; D002417:Cattle; D045304:Cytochromes c / Q000737:chemistry; D004795:Enzyme Stability; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry; D005782:Gels; D006361:Heating; D009211:Myoglobin / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D040901:Proteomics; D011887:Raffinose / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014312:Trisaccharides / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry*
chemical_list: D005782:Gels; D009211:Myoglobin; D009844:Oligosaccharides; D012996:Solutions; D014312:Trisaccharides; C005695:stachyose; D012710:Serum Albumin, Bovine; C008317:maltotriose; D045304:Cytochromes c; D014357:Trypsin; D005634:Fructose-Bisphosphate Aldolase; D011887:Raffinose