The metabolism and biological activity of a 9-substituted cytokinin.
In order to test the metabolic stability of 9-substituted cytokinins, 6-benzylamino-9-methyl purine has been synthesized and labeled with (14)C in the 9-methyl carbon or doubly labeled with (14)C in the 9-methyl carbon and (3)H in the methylene moiety of the side chain. Although the 6-benzylamino-9-methylpurine is chemically stable, cytokinin-requiring tissues begin removing the 9-substituent in as little as 10 minutes. Among the various metabolic products is free benzylaminopurine. Thus, the biological activity of 9-substituted cytokinins could be accounted for by their conversion to the free base.
Fox
J E
JE
Department of Botany, The University of Kansas, Lawrence, Kansas 66044.
Sood
C K
CK
Buckwalter
B
B
McChesney
J D
JD