title: Evaluation of a novel strip test, GenoType Mycobacterium CM/AS, for species identification of mycobacterial cultures.
abstract: A novel DNA strip assay, GenoType Mycobacterium AS, was evaluated for its ability to identify 219 mycobacterial isolates in combination with the GenoType Mycobacterium CM assay. The results were compared with those obtained by conventional 16S rDNA sequencing. The Genotype test correlated well (96%) with sequencing. However, with the CM kit alone, it was possible to identify most (88%) of the isolates found in clinical specimens, and the AS kit provided very little additional information.
authors.affiliation: Mycobacterial Reference Laboratory, National Public Health Institute, Kiinamyllynkatu 13, 20520 Turku, Finland. johanna.makinen@ktl.fi
authors.forename: J
authors.initials: J
authors.lastname: Mäkinen
authors.forename: M
authors.initials: M
authors.lastname: Marjamäki
authors.forename: H
authors.initials: H
authors.lastname: Marttila
authors.forename: H
authors.initials: H
authors.lastname: Soini
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S; D011933:Reagent Kits, Diagnostic