[Research progress on trehalose used in lyophilization of blood cells--review].
Lyophilization is the best method for preservation of blood cells at present. Lyophilized blood cells could be stored at room temperature for long periods of time, while maintaining a high degree of viability. Lyophilized blood cells facilitates transportation and the costs are low. However, the membrane of blood cells is damaged and viability of blood cells is decreased in lyophilization. Trehalose has been shown to protect membrane, proteins and nucleic acids during freezing and desiccation. Now, researchers present a methor for loading blood cells with trehalose. In this paper, damage effect of lyophilization on blood cells, the mechanism of trehalose protection and the experimental studies on trehalose were reviewed.
Chen
Yan
Y
Department of Blood Transfusion, Zhujiang Hospital, The Souther Medical University, Guangzhou 510282, China.
Lu
Zhi-Gang
ZG
D001793:Blood Preservation / Q000379:methods*; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects; D005612:Freeze Drying / Q000379:methods*; D006801:Humans; D014199:Trehalose / Q000494:pharmacology*
D003451:Cryoprotective Agents; D014199:Trehalose