Gas-liquid chromatography of trimethylsilyl derivatives of abscisic Acid and other plant hormones.
This paper describes a new method for qualitative and quantitative assay of abscisic acid and other acidic plant hormones, such as indoleacetic acid and the gibberellins, by the gas-liquid chromatography of their trimethylsilyl derivatives. Interfering substances in plant extracts were largely removed by preliminary column chromatography with carbon-celite and elution of the abscisic acid with 60% acetone, permitting direct determination of abscisic acid by gas-liquid chromatography using a flame ionization detector. (A level of 0.65 mg/kg fr wt was found.) This method enables measurement of amounts of abscisic acid as low as 0.025 mug. In impure samples collected by gas-liquid chromatography the abscisic acid recovered could be measured quantitatively by use of its ultraviolet absorption maximum at 260 mmu.
Davis
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Department of Agronomy, University of California, Davis, California 95616.
Heinz
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Addicott
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