title: Hydrophilic nanofibrous structure of polylactide; fabrication and cell affinity.
abstract: Microstructure and architecture of the scaffolds along with the surface chemistry exert profound effect on biological activity (cell distribution, proliferation, and differentiation). For the biological activity, scaffolds in tissue engineering have been widely designed. The objective of this study was to develop hydrophilic nanofibrous structure of polylactides (PLLA) polymer in the form of nonwoven mat by electrospinning technique, and further evaluate the fibroblast NIH3T3 cell proliferation, morphology, and cell-matrix interaction. Hydrophilicity of the PLLA fibers was improved by adding small fraction of low molecular weight polyethylene glycol (PEG) into the electrospinning solution. Four different ratio types (100/0, 80/20, 70/30, and 50/50) of PLLA/PEG electrospun matrices were fabricated, and the pore characteristics, tensile properties, contact angle, and hydrolytic degradation were observed. Furthermore, scanning electron microscope (SEM) and fluorescence actin staining images were used for micro-observation of cell-matrix interaction and cell morphology. It was found that the electrospun mat of PLLA/PEG (80/20), composed of fibers with diameters in the range 540-850 nm, majority of pore diameter less than 100 microm, tensile strength 8 MPa, elongation 150%, porosity more than 90%, and improved hydrophilicity with slow hydrolytic degradation, is favorable for biological activity of NIH3T3 fibroblast cell. Based on these results, the correct composition of PLLA and PEG in the porous electrospun matrix (i.e., PLLA/PEG (80/20)) will be a better candidate rather than other compositions of PLLA/PEG as well as hydrophobic PLLA for application in tissue engineering.
authors.affiliation: Department of Bio-nanosystem engineering, Chonbuk National University, Chonju 561-756, Republic of Korea.
authors.forename: Shanta Raj
authors.initials: SR
authors.lastname: Bhattarai
authors.forename: Narayan
authors.initials: N
authors.lastname: Bhattarai
authors.forename: Periasamy
authors.initials: P
authors.lastname: Viswanathamurthi
authors.forename: Ho Keun
authors.initials: HK
authors.lastname: Yi
authors.forename: Pyoung Han
authors.initials: PH
authors.lastname: Hwang
authors.forename: Hak Yong
authors.initials: HY
authors.lastname: Kim
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002468:Cell Physiological Phenomena; D051379:Mice; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D011092:Polyethylene Glycols; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011092:Polyethylene Glycols; C033616:poly(lactide)