Expression profiling of genes encoding glutamate and GABA receptor subunits in three immortalized GnRH cell lines.
Gonadotropin-releasing hormone (GnRH) plays a central role in regulating development and function of the reproductive axis, and its secretion is known to be influenced by glutamate and GABA. In the present study, we used gene microarrays and RT-PCR to compare the expression profiles of glutamate and GABA receptor subunits in three immortalized GnRH cell lines: GT1-1, GT1-7, and Gn10. All of these cell lines expressed the AMPA glutamate receptor subunit genes GluR2 and GluR4, but only the GT1-1 and GT1-7 cells expressed the kainate glutamate receptor subunit gene KA2. Additionally, GluRdelta2, a subunit that can form heteromeric receptors with kainate and AMPA subunits, was present in GT1-1 and Gn10 cells but not in GT1-7 cells. Genes encoding the GABA(A) receptor alpha3, beta2, beta3, epsilon, and pi subunits, as well as the GABA(B) receptor 1 subunit, were evident in all three cell lines. However, the gene encoding the expression of GABA(A) receptor gamma subunit was noticeably absent. Taken together, these data demonstrate comprehensive screening of neurotransmitter receptor genes in a controlled neuronal culture system, and reveal novel features.
Garyfallou
Vasilios T
VT
Division of Neuroscience, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, 97006, USA.
Lemos
Dario
D
Urbanski
Henryk F
HF
D000818:Animals; D002461:Cell Line, Transformed; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D018079:Receptors, GABA / Q000235:genetics / Q000378:metabolism*; D017470:Receptors, Glutamate / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D012333:RNA, Messenger; D018079:Receptors, GABA; D017470:Receptors, Glutamate; D007987:Gonadotropin-Releasing Hormone