[Repair of canine segmental mandibular defects using autogenous bone marrow stromal cells and coralline hydroxyapatite].
OBJECTIVE
To repair segmental mandibular defects with autogenous bone marrow stromal cells (BMSCs) and coralline hydroxyapatite.


METHODS
Isolated BMSCs were in vitro expanded and osteogenically induced. In 11 canines, a 3 cm segmental mandibular defect in right mandible was created. Five canine's defects were repaired with cell-scaffold constructs made from induced BMSCs and coralline hydroxyapatite (CHA); Others were repaired with CHA as control. The engineered bone was evaluated by X-ray, CT, gross and histological examination, biomechanical test 12, 26, 32 weeks post-operation respectively.


RESULTS
BMSCs grew well on the CHA. X-ray and CT images showed better callus formation at connection sites in experimental group over time while worse formation at connection sites eventually in control group. At 32 weeks post-operation in experimental group, the defects were well repaired grossly. Histologically, there were bony healing and lamellar bone formation, in experimental group fibrous healing and woven bone formation in control group. Biomechanical test revealed no significant difference between experimental group and normal control group.


CONCLUSIONS
Canine segmental mandibular defects can be ultimately repaired with the tissue-engineered bone generated by autogenous osteogenic BMSCs and CHA scaffold.
Yuan
Jie
J
Department of Plastic and Reconstructive Surgery, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Tissue Engineering, Shanghai 200011, China.
Zhu
Lian
L
Wang
Min
M
Cui
Lei
L
Liu
Wei
W
Cao
Yi-lin
YL
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D018786:Bone Substitutes; D002516:Ceramics / Q000627:therapeutic use*; D004285:Dogs; D006882:Hydroxyapatites / Q000627:therapeutic use*; D008334:Mandible / Q000502:physiology; D008338:Mandibular Injuries / Q000473:pathology / Q000601:surgery*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D018786:Bone Substitutes; D006882:Hydroxyapatites; C119955:coralline hydroxyapatite