A highly flexible tRNA acylation method for non-natural polypeptide synthesis.
Here we describe a de novo tRNA acylation system, the flexizyme (Fx) system, for the preparation of acyl tRNAs with nearly unlimited selection of amino and hydroxy acids and tRNAs. The combination of the Fx system with an appropriate cell-free translation system allows us to readily perform mRNA-encoded synthesis of proteins and short polypeptides involving multiple non-natural amino acids.
Murakami
Hiroshi
H
Research Center for Advanced Science and Technology, The University of Tokyo, 153-8904 Tokyo, Japan.
Ohta
Atsushi
A
Ashigai
Hiroshi
H
Suga
Hiroaki
H
D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis*; D015202:Protein Engineering / Q000379:methods*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D046249:Transfer RNA Aminoacylation
D000596:Amino Acids; D009842:Oligopeptides; D012333:RNA, Messenger; D012343:RNA, Transfer