title: Predicting stochastic gene expression dynamics in single cells.
abstract: Fluctuations in protein numbers (noise) due to inherent stochastic effects in single cells can have large effects on the dynamic behavior of gene regulatory networks. Although deterministic models can predict the average network behavior, they fail to incorporate the stochasticity characteristic of gene expression, thereby limiting their relevance when single cell behaviors deviate from the population average. Recently, stochastic models have been used to predict distributions of steady-state protein levels within a population but not to predict the dynamic, presteady-state distributions. In the present work, we experimentally examine a system whose dynamics are heavily influenced by stochastic effects. We measure population distributions of protein numbers as a function of time in the Escherichia coli lactose uptake network (lac operon). We then introduce a dynamic stochastic model and show that prediction of dynamic distributions requires only a few noise parameters in addition to the rates that characterize a deterministic model. Whereas the deterministic model cannot fully capture the observed behavior, our stochastic model correctly predicts the experimental dynamics without any fit parameters. Our results provide a proof of principle for the possibility of faithfully predicting dynamic population distributions from deterministic models supplemented by a stochastic component that captures the major noise sources.
authors.affiliation: Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Jerome T
authors.initials: JT
authors.lastname: Mettetal
authors.forename: Dale
authors.initials: D
authors.lastname: Muzzey
authors.forename: Juan M
authors.initials: JM
authors.lastname: Pedraza
authors.forename: Ertugrul M
authors.initials: EM
authors.lastname: Ozbudak
authors.forename: Alexander
authors.initials: A
authors.lastname: van Oudenaarden
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D005786:Gene Expression Regulation; D007785:Lactose / Q000378:metabolism*; D008954:Models, Biological; D013269:Stochastic Processes
chemical_list: D007785:Lactose