title: A slide latex agglutination test for the rapid detection of antibodies in serum against porcine parvovirus.
abstract: A slide latex agglutination test (LAT) was developed and evaluated to detect serum antibodies against porcine parvovirus. Porcine parvovirus antigen was obtained by 10% PEG-6000 and 0.5 mol/l sodium chloride precipitation, and inactivated by 0.1% methanal. Two per cent suspensions of latex particles (0.5-0.8 microm) were coated by adding an equal volume of porcine parvovirus antigen at 0.34 microg/ml. Repeatability of latex agglutination test was evaluated with a panel of 100 sera using the same and different antigen lots. A good agreement between LAT and haemagglutination inhibit assay was observed. Because of convenience and speed of performance, this method would be used widely in clinic examination.
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: J
authors.initials: J
authors.lastname: Lü
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: L
authors.initials: L
authors.lastname: Fang
authors.forename: Q
authors.initials: Q
authors.lastname: He
authors.forename: S
authors.initials: S
authors.lastname: Cao
authors.forename: H
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D007841:Latex Fixation Tests / Q000379:methods / Q000662:veterinary*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D028323:Parvovirus, Porcine / Q000276:immunology*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D013997:Time Factors
chemical_list: D000914:Antibodies, Viral