Characterization of a new, nonpathogenic mutant of Botrytis cinerea with impaired plant colonization capacity.
Botrytis cinerea is a necrotrophic pathogen that attacks more than 200 plant species. Here, the nonpathogenic mutant A336, obtained via insertional mutagenesis, was characterized. Mutant A336 was nonpathogenic on leaves and fruits, on intact and wounded tissue, while still able to penetrate the host plant. It grew normally in vitro on rich media but its conidiation pattern was altered. The mutant did not produce oxalic acid and exhibited a modified regulation of the production of some secreted proteins (acid protease 1 and endopolygalacturonase 1). Culture filtrates of the mutant triggered an important oxidative burst in grapevine (Vitis vinifera) suspension cells, and the mutant-plant interaction resulted in the formation of hypersensitive response-like necrosis. Genetic segregation analyses revealed that the pathogenicity phenotype was linked to a single locus, but showed that the mutated gene was not tagged by the plasmid pAN7-1. Mutant A336 is the first oxalate-deficient mutant to be described in B. cinerea and it differs from all the nonpathogenic B. cinerea mutants described to date.
Kunz
Caroline
C
Laboratoire Interactions Plantes-Pathogènes (UMR 217 INRA/INA-PG/Université Pierre et Marie Curie), INA-PG, 16 rue Claude Bernard, 75231 Paris Cedex 05, France. kunz@ccr.jussieu.fr
Vandelle
Elodie
E
Rolland
Stéphane
S
Poinssot
Benoit
B
Bruel
Christophe
C
Cimerman
Agnès
A
Zotti
Corine
C
Moreau
Elisabeth
E
Vedel
Régine
R
Pugin
Alain
A
Boccara
Martine
M
D017360:Arabidopsis / Q000033:anatomy & histology / Q000382:microbiology / Q000502:physiology; D020171:Botrytis / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D004798:Enzymes / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D015966:Gene Expression Regulation, Fungal; D006863:Hydrogen-Ion Concentration; D007113:Immunity, Innate / Q000502:physiology; D016254:Mutagenesis, Insertional; D009154:Mutation; D019697:Onions / Q000166:cytology / Q000382:microbiology; D019815:Oxalic Acid / Q000378:metabolism; D027805:Phaseolus / Q000033:anatomy & histology / Q000382:microbiology / Q000502:physiology; D018515:Plant Leaves / Q000033:anatomy & histology / Q000382:microbiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D027843:Vitis / Q000033:anatomy & histology / Q000382:microbiology* / Q000502:physiology
D004798:Enzymes; D005656:Fungal Proteins; D017382:Reactive Oxygen Species; D019815:Oxalic Acid