Litter-forager termite mounds enhance the ectomycorrhizal symbiosis between Acacia holosericea A. Cunn. Ex G. Don and Scleroderma dictyosporum isolates.
The hypothesis of the present study was that the termite mounds of Macrotermes subhyalinus (MS) (a litter-forager termite) were inhabited by a specific microflora that could enhance with the ectomycorrhizal fungal development. We tested the effect of this feeding group mound material on (i) the ectomycorrhization symbiosis between Acacia holosericea (an Australian Acacia introduced in the sahelian areas) and two ectomycorrhizal fungal isolates of Scleroderma dictyosporum (IR408 and IR412) in greenhouse conditions, (ii) the functional diversity of soil microflora and (iii) the diversity of fluorescent pseudomonads. The results showed that the termite mound amendment significantly increased the ectomycorrhizal expansion. MS mound amendment and ectomycorrhizal inoculation induced strong modifications of the soil functional microbial diversity by promoting the multiplication of carboxylic acid catabolizing microorganisms. The phylogenetic analysis showed that fluorescent pseudomonads mostly belong to the Pseudomonads monteillii species. One of these, P. monteillii isolate KR9, increased the ectomycorrhizal development between S. dictyosporum IR412 and A. holosericea. The occurrence of MS termite mounds could be involved in the expansion of ectomycorrhizal symbiosis and could be implicated in nutrient flow and local diversity.
Duponnois
Robin
R
Institut de Recherche pour le Développement, Laboratoire des Symbioses Tropicales et Méditerranéennes, Montpellier, France. robin.duponnois@ird.sn
Assikbetse
Komi
K
Ramanankierana
Heriniaina
H
Kisa
Marija
M
Thioulouse
Jean
J
Lepage
Michel
M
D000045:Acacia / Q000382:microbiology*; D000818:Animals; D001487:Basidiomycota / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D044822:Biodiversity; D005838:Genotype; D020049:Isoptera / Q000382:microbiology*; D038821:Mycorrhizae / Q000254:growth & development / Q000502:physiology*; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012988:Soil Microbiology; D013559:Symbiosis