title: Characterization of adhesin variants in Indian isolates of enteroaggregative Escherichia coli.
abstract: Enteroaggregative Escherichia coli (EAEC) are causative agents of diarrhea, being characterized by aggregative adherence to cultured epithelial cells. In this study, phenotypic properties of EAEC were analyzed with respect to AA, hemagglutination, clump and biofilm formation, all of which are mediated by aggregative adherence fimbriae (AAF). The strains were also screened for AAF types, AAF adhesin variants and Dr adhesin by PCR. Of the three known AAF types, AAF/I and AAF/II adhesin variants were identified. An association between the AAF/adhesin genotypes and the subtypes/scores of phenotypic properties was sought and it was observed that strains harboring same adhesins displayed different subtypes/scores and vice versa.
authors.affiliation: Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Bhardwaj
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Majumdar
authors.forename: Nirmal K
authors.initials: NK
authors.lastname: Ganguly
authors.forename: Neelam
authors.initials: N
authors.lastname: Taneja
authors.forename: Shanta
authors.initials: S
authors.lastname: Dutta
authors.forename: Thanadavarayan
authors.initials: T
authors.lastname: Ramamurthy
authors.forename: Anuradha
authors.initials: A
authors.lastname: Chakraborti
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development; D002675:Child, Preschool; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D036781:Fimbriae Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D010861:Fimbriae, Bacterial / Q000145:classification / Q000378:metabolism; D005838:Genotype; D006384:Hemagglutination / Q000235:genetics; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D010641:Phenotype; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
chemical_list: C108511:AafA protein, E coli; D018830:Adhesins, Escherichia coli; C079725:AggA protein, E coli; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins