Pairing of heterochromatin in response to cellular stress.
We previously reported that exposure of human cells to DNA-damaging agents (X-rays and mitomycin C (MMC)) induces pairing of the homologous paracentromeric heterochromatin of chromosome 9 (9q12-13). Here, we show that UV irradiation and also heat shock treatment of human cells lead to similar effects. Since the various agents induce very different types and frequencies of damage to cellular constituents, the data suggest a general stress response as the underlying mechanism. Moreover, local UV irradiation experiments revealed that pairing of heterochromatin is an event that can be triggered without induction of DNA damage in the heterochromatic sequences. The repair deficient xeroderma pigmentosum cells (group F) previously shown to fail pairing after MMC displayed elevated pairing after heat shock treatment but not after UV exposure. Taken together, the present results indicate that pairing of heterochromatin following exposure to DNA-damaging agents is initiated by a general stress response and that the sensing of stress or the maintenance of the paired status of the heterochromatin might be dependent on DNA repair.
Abdel-Halim
H I
HI
Department of Toxicogenetics, Leiden University Medical Center, PO Box 9503, 2300 RA Leiden, The Netherlands.
Mullenders
L H F
LH
Boei
J J W A
JJ
D002478:Cells, Cultured; D023902:Chromosome Pairing / Q000235:genetics / Q000528:radiation effects*; D002875:Chromosomes / Q000502:physiology / Q000528:radiation effects; D004249:DNA Damage / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology* / Q000528:radiation effects*; D018869:Heat-Shock Response; D006570:Heterochromatin / Q000502:physiology* / Q000528:radiation effects; D006801:Humans; D007399:Interphase / Q000528:radiation effects; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics
D006570:Heterochromatin