title: Effect of JH III and substituted oxime ethers on the in vitro protein and RNA synthesis in male accessory reproductive gland (MARG) of Spodoptera litura (F.).
abstract: The physiological action of two substituted oxime ethers namely: 4'-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3'-buten-2'(E)-ketoxime-N-O-propylether (compound No. 3) and 4'-(2,6,6-trimethyl-1-cyclohexen-1-yl)-3'-buten-2'(E)-ketoxime-N-O-pentylether (compound No. 34,) were compared with that of JH III in an in vitro assay to monitor the synthesis of RNA and protein in male accessory reproductive gland (MARG) of Spodoptera litura by using 3H-leucine and 3H-uridine, respectively. Both the compounds have stimulated protein synthesis compare to control. Compound No 34 is slightly more effective than JH III in increasing the protein synthesis at physiological concentration of 10(-6) and 10(-5) M. Compound No 3 and JH III have doubled the RNA synthesis and increased the protein synthesis by 1.5 times over the control at 10(-4) to 10(-6) M concentrations. While JH III at 10(-5) M significantly enhanced RNA synthesis, similar effect is produced only at 10(-3) M by compound No 3 and 34.
authors.affiliation: Division of Plant Protection, Faculty of Agriculture, Valie Asr University Rafsanjan, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Izadi
authors.forename: B
authors.initials: B
authors.lastname: Subrahmanyam
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005837:Genitalia, Male / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010091:Oximes / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012313:RNA / Q000096:biosynthesis*; D012717:Sesquiterpenes / Q000494:pharmacology*; D013045:Species Specificity; D018411:Spodoptera / Q000378:metabolism*; D014316:Tritium
chemical_list: D010091:Oximes; D012717:Sesquiterpenes; D014316:Tritium; D012313:RNA; C036585:juvenile hormone III