title: Inhibition of putrescine aminopropyltransferase influences rat liver regeneration.
abstract: A close relationship between rat liver regeneration and the concentration ratio of spermidine to spermine (spd:spm) was demonstrated by the oral administration of trans-4-methylcyclohexylamine (MCHA), a specific inhibitor of putrescine aminopropyltransferase. A decrease in recovery rate of remnant liver with MCHA, as a percentage index of remnant liver weight to body weight, correlated well with a decrease of the spd:spm value, with a correlation coefficient of 0.952 for the remnant livers on day 3 after partial hepatectomy. The decrease in recovery rate could be explained by a prolonged cell cycle based on the data of the proliferating cell nuclear antigen labelling index and mitotic cell index in both livers of day 2 and day 3 after partial hepatectomy. The results presented here will give a new aspect in the field of polyamine regulation to control cell growth in vivo.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Koichi
authors.initials: K
authors.lastname: Takao
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Shiota
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sugita
authors.forename: Masakazu
authors.initials: M
authors.lastname: Takahashi
authors.forename: Dai
authors.initials: D
authors.lastname: Nakae
authors.forename: Keijiro
authors.initials: K
authors.lastname: Samejima
mesh_terms: D000818:Animals; D015317:Biogenic Polyamines / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003514:Cyclohexylamines / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D008938:Mitosis / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011436:Spermidine Synthase / Q000037:antagonists & inhibitors*
chemical_list: D015317:Biogenic Polyamines; D003514:Cyclohexylamines; D004791:Enzyme Inhibitors; C059116:4-methylcyclohexylamine; D011436:Spermidine Synthase