Electroacupuncture suppresses expression of gastric ghrelin and hypothalamic NPY in chronic food restricted rats.
Electroacupuncture (EA) has been reported to reduce body weight in overweight subjects in clinical practice, as well as in rats and mice with diet-induced obesity. In the present study, this effect of EA was tested in lean rats subjected to long-term food restriction (FR, food was offered only 1 h/day). Two hertz EA administered once every other day produced a further reduction in body weight in FR rats. Exploration of the mechanism involved revealed significant downregulation of the orexigenic peptides: ghrelin in the stomach, and neuropeptide Y (NPY) but not Agouti-related peptide (AgRP) in the hypothalamus, which was in line with the reduction in food intake in rats receiving EA stimulation as compared with those receiving restraint only. Uncoupling protein 3 (UCP3), involved in accelerating energy expenditure, was not significantly altered. These results suggest that the EA-induced body weight reduction was due mainly to a decrease in food intake rather than an increase in energy expenditure. A reduction in the orexigenic peptides ghrelin and NPY may be involved in the underlying mechanism.
Tian
Nan
N
Neuroscience Research Institute, Peking University, Beijing 100083, PR China.
Wang
Fei
F
Tian
De-Run
DR
Zou
Yuan
Y
Wang
Shi-Wei
SW
Guan
Li-Li
LL
Shi
Yu-Shun
YS
Chang
Jaw-Kang
JK
Yang
Jun
J
Han
Ji-Sheng
JS
D054369:Agouti-Related Protein; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000502:physiology; D004435:Eating / Q000502:physiology; D015671:Electroacupuncture; D005508:Food Deprivation; D005753:Gastric Mucosa / Q000378:metabolism*; D054439:Ghrelin; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D024101:Mitochondrial Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism*; D036361:Peptide Hormones / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000166:cytology; D014280:Triglycerides / Q000032:analysis / Q000097:blood; D000071247:Uncoupling Protein 3
D054369:Agouti-Related Protein; D001786:Blood Glucose; D054439:Ghrelin; D036341:Intercellular Signaling Peptides and Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; D009478:Neuropeptide Y; D036361:Peptide Hormones; D012333:RNA, Messenger; D014280:Triglycerides; C000604393:Ucp3 protein, mouse; C000604394:Ucp3 protein, rat; D000071247:Uncoupling Protein 3