Facile synthesis and application of uniformly 13C, 15N-labeled phosphotyrosine for ligand binding studies.
The first synthesis of [U-13C, 15N] labeled phosphotyrosine is described. Preliminary studies toward the binding of phosphotyrosine to an SH2 domain have been performed by means of heteronuclear NMR.
Tuin
Adriaan W
AW
Leiden Institute of Chemistry, Gorlaeus Laboratories, PO Box 9502, 2300 RA Leiden, The Netherlands.
Siegal
Gregg
G
van der Marel
Gijsbert A
GA
Overkleeft
Herman S
HS
Filippov
Dmitri V
DV
D001665:Binding Sites; D002247:Carbon Isotopes; D007553:Isotope Labeling / Q000379:methods; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008956:Models, Chemical; D009587:Nitrogen Isotopes; D019000:Phosphotyrosine / Q000138:chemical synthesis* / Q000378:metabolism; D018909:src Homology Domains / Q000502:physiology
D002247:Carbon Isotopes; D008024:Ligands; D009587:Nitrogen Isotopes; D019000:Phosphotyrosine