title: Singlepath Salmonella. Performance-Tested Method 060401.
abstract: Singlepath Salmonella is an immunochromatographic (lateral flow) assay for the presumptive qualitative detection of Salmonella spp. in food. The AOAC Performance-Tested Method study evaluated Singlepath Salmonella as an effective method for the detection of Salmonella spp. in the following selected foods: dried skimmed milk, black pepper, dried pet food, desiccated coconut, cooked peeled frozen prawns, raw ground beef, and raw ground turkey. When the foods were inoculated with Salmonella spp. at levels ranging from low [0.23-1.08 colony forming units (CFU)/25 g] to high (2.3-6.0 CFU/25 g), a Chi-square value of 0.9 indicated that there was no significant difference between Singlepath Salmonella and the ISO 6579:2002 reference method. Singlepath Salmonella gave a false-positive rate of 7.3% and a false-negative rate of 2.5%. For the inclusivity study, all 105 Salmonella serovars reacted with Singlepath Salmonella. For the exclusivity study, 58 non-Salmonella spp. were tested. There were no cross-reactions with Singlepath Salmonella from these strains.
authors.affiliation: Merck Chemicals Ltd., Newport Technical Centre, 5 The Courtyard, Imperial Park, Newport NP10 8UL, Wales, United Kingdom. lisa.thompson@merckchem.co.uk
authors.forename: Lisa
authors.initials: L
authors.lastname: Thompson
authors.forename: Charlotte
authors.initials: C
authors.lastname: Lindhardt
mesh_terms: D001419:Bacteria / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007120:Immunochemistry; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results; D012475:Salmonella / Q000737:chemistry*; D017747:Seafood / Q000382:microbiology
chemical_list: D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic