Effect of Gusuibu graft on bone formation.
OBJECTIVE
We compared the amount of new bone produced by Gusuibu in collagen grafts to that produced by bone grafts and collagen grafts.


METHODS
Twenty bone defects were created in the parietal bone of 14 New Zealand White rabbits. In the experimental group, 5 defects were grafted with Gusuibu extract mixed with absorbable collagen sponge, and 5 defects were grafted with autogenous endochondral bone. In the control groups, 5 defects were grafted with absorbable collagen sponge alone (active control) and 5 were left empty (passive control). Animals were killed on day 14 and the defects were dissected and prepared for histologic assessment. Serial sections were cut across each defect. Quantitative analysis of new bone formation was made on 150 sections using image analysis.


RESULTS
A total of 24% and 90% more new bone were present in defects grafted with Gusuibu in collagen grafts than those grafted with bone and collagen, respectively. No bone was formed in the passive control group.


CONCLUSIONS
Gusuibu in collagen grafts have the effect of increased new bone formation locally and can be used as a bone graft material.
Wong
Ricky W K
RW
Department of Orthodontics, University of Hong Kong, Prince Philip Dental Hospital, Sai Ying Pun, Hong Kong. fyoung@hkucc.hku.hk
Rabie
A Bakr M
AB
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects*; D016025:Bone Transplantation / Q000379:methods; D003094:Collagen / Q000008:administration & dosage / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D010012:Osteogenesis / Q000187:drug effects*; D010294:Parietal Bone / Q000187:drug effects* / Q000378:metabolism / Q000601:surgery; D029621:Polypodiaceae; D011817:Rabbits; D027343:Rhizome
D001672:Biocompatible Materials; D004365:Drugs, Chinese Herbal; D003094:Collagen