title: Enhanced deoxyribonucleic Acid polymerase activity of chromatin from soybean hypocotyls treated with 2,4-dichlorophenoxyacetic Acid.
abstract: Chromatin isolated from soybean (Glycine max L., var. Wayne) hypocotyls was capable of catalyzing the polymerization of labeled deoxyribonucleoside triphosphate in the presence of the three other deoxyribonucleoside triphosphates into a trichloroacetic acid-insoluble product. This product was insensitive to base hydrolysis and ribonuclease, but was sensitive to acid hydrolysis and deoxyribonuclease. Chromatin-DNA polymerase required Mg(2+) and all four deoxyribonucleoside triphosphates for maximal activity. Inorganic pyrophosphate and actinomycin D inhibited the polymerase activity, but 2, 4-dichlorophenoxyacetic acid had no effect in vitro. Chromatin from plants previously treated with 2, 4-dichlorophenoxyacetic acid supported a greater level of DNA synthesis than did chromatin from untreated plants.
authors.affiliation: Departments of Agronomy and Horticulture, Purdue University, Lafayette, Indiana 47907.
authors.forename: H R
authors.initials: HR
authors.lastname: Leffler
authors.forename: T J
authors.initials: TJ
authors.lastname: O'brien
authors.forename: D V
authors.initials: DV
authors.lastname: Glover
authors.forename: J H
authors.initials: JH
authors.lastname: Cherry