Microdialysis measurement of acetylcholine in rat hippocampus during severe insulin-induced hypoglycemia.
OBJECTIVE
It has been well acknowledged that hypoglycemia induces cognitive impairment. However, the behavior of hippocampal cholinergic system, which is thought to play a key role in cognition, during hypoglycemia remains to be elucidated.


METHODS
In this study we conducted a microdialysis using 9 weeks old virgin male Wistar/ST rats to determine the changes of extracelluar acethylcholine and glucose levels in the hippocampus during insulin-induced severe hypoglycemia, and compared the results with those obtained from a microdialysis in the striatum.


RESULTS
The extracelluar concentrations of acethylcholine both in the hippocampus and striatum did not change during hypoglycemia.


CONCLUSIONS
The results of the present study may suggest that changes of hippocampal cholinergic release may not be involved in the mechanism of cognitive impairment during hypoglycemia.
Umegaki
Hiroyuki
H
Department of Geriatrics, Nagoya University Graduate School of Medicine, 65 Tsuruma-Cho, Showa-Ku, Nagoya, Aichi 466-8550, Japan. umegaki@med.nagoya-u.ac.jp
Yamaguchi
Yoko
Y
Suzuki
Yusuke
Y
Iguchi
Akihisa
A
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D007003:Hypoglycemia / Q000139:chemically induced* / Q000378:metabolism*; D007004:Hypoglycemic Agents; D007328:Insulin; D008297:Male; D017551:Microdialysis; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D000109:Acetylcholine