title: Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma.
abstract: Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10,000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.
authors: Department of Clinical and Experimental Medicine, Magna Graecia University, 88100 Catanzaro, Italy. Gaspari@unicz.it
authors: Marco
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authors: Gaspari
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authors: Ming-Cheng Cheng
authors: Rosa
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authors: Terracciano
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authors: Liu
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authors: Vaccari
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authors: di Fabrizio
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