Selection of diquat resistance photosynthesis mutants from maize.
Resistance of a seedling to the herbicide 1,1'-ethylene-2,2'-dipyridylium bromide (diquat) can be used as a selective technique for photosynthesis mutants in Zea mays L. Diquat requires reduction by the light reaction in order to kill leaf cells and, therefore, nonphotosynthetic mutants survive. This technique was tested using known mutants and is applicable to larger samples of plants than previous techniques. Resistance to diquat should allow selection of mutants on the oxidizing side of photosystem II which are not previously available in higher plants.
Miles
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Biological Sciences, University of Missouri, Columbia, Missouri 65201.