A rapid, sensitive method for quantitating subunits in purified ribulose bisphosphate carboxylase preparations.
Studies of the interactions of the large and small subunits of ribulose bisphosphate carboxylase require a knowledge of the concentrations of the subunits present in various preparations and their ratio. Since existing sodium dodecyl sulfate-gel electrophoresis procedures proved quantitatively unreliable, a technique based on high performance-gel filtration was developed. The latter is most reliable, takes only about 30 minutes to perform, and detects a minimum of 0.25 micrograms of each subunit.
Andrews
T J
TJ
Australian Institute of Marine Science, PMB No. 3, TMC, Townsville, Australia 4810.
Ballment
B
B