title: Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection.
abstract: We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis of B. pertussis infection even in clinical laboratories with no specific equipment.
authors: Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Musashimurayma-city, Tokyo 208-0011, Japan. kamachi@nih.go.jp
authors: Kazunari
authors: K
authors: Kamachi
authors: Hiromi
authors: H
authors: Toyoizumi-Ajisaka
authors: Kohei
authors: K
authors: Toda
authors: Sann Chan
authors: SC
authors: Soeung
authors: Svay
authors: S
authors: Sarath
authors: Ya
authors: Y
authors: Nareth
authors: Yoshinobu
authors: Y
authors: Horiuchi
authors: Kazunobu
authors: K
authors: Kojima
authors: Motohide
authors: M
authors: Takahashi
authors: Yoshichika
authors: Y
authors: Arakawa