Agrobacterium-mediated transformation of Petunia leaf discs.
Many dicotyledonous and also several monocotyledonous plant species are susceptible to Agrobacterium-mediated transformation. This current and well-established method has been used successfully with a large number of plant species to mediate gene transfer. This chapter describes an Agrobacterium-mediated transformation method of Petunia hybrida leaf discs, one of the first species that was routinely transformed using this method.
van der Meer
Ingrid M
IM
Business Unit Bioscience, Plant Research International B.V., Wageningen, The Netherlands.