title: Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin.
abstract: We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%).
authors: CIISA, Faculty of Veterinary Medicine, Universidade Técnica de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal. cpomba@fmv.utl.pt
authors: Constança
authors: C
authors: Pomba
authors: Nuno
authors: N
authors: Mendonça
authors: Marta
authors: M
authors: Costa
authors: Deolinda
authors: D
authors: Louro
authors: Bruno
authors: B
authors: Baptista
authors: Mónica
authors: M
authors: Ferreira
authors: José Duarte
authors: JD
authors: Correia
authors: Manuela
authors: M
authors: Caniça