title: Isolation of cytoplasmic enzymes from pollen.
abstract: The cytoplasmic isozyme of many cytoplasmic-organelle isozyme pairs, as well as other cytoplasmic enzymes in plants, can be readily obtained from pollen by soaking it in an appropriate buffer for 4 hours. Enzymes localized in subcellular organelles appear not to be released during the soaking period, although they are released if the pollen is crushed. The technique is a useful initial step in studies of subcellular localization of enzymes or for obtaining small quantities of cytoplasmic enzymes free of organellar contaminants.
authors: Department of Genetics, University of California, Davis, California 95616.
authors: N F
authors: NF
authors: Weeden
authors: L D
authors: LD
authors: Gottlieb