title: Spin-density distribution in the copper site of azurin.
abstract: A 95 GHz pulsed deuterium ENDOR study has been performed on single crystals of azurin from Pseudomonas aeruginosa selectively deuterated at the C(beta) position of the copper-coordinating cysteine 112. Complete hyperfine tensors of the two deuterium atoms have been obtained, which reveal identical isotropic parts. Analysis of the hyperfine tensors provides insight into the spin-density delocalization over the cysteine ligand. Approximately 45 % of the spin density in the paramagnetic site can be attributed to copper and 30 % to sulfur.
authors: Department of Molecular Physics, Huygens Laboratory, Leiden University, P.O. Box 9504, 2300 RA Leiden, The Netherlands. mat@molphys.leidenuniv.nl
authors: Maria
authors: M
authors: Fittipaldi
authors: Gertrud C M
authors: GC
authors: Warmerdam
authors: Ellen C
authors: EC
authors: de Waal
authors: Gerard W
authors: GW
authors: Canters
authors: Davide
authors: D
authors: Cavazzini
authors: Gian Luigi
authors: GL
authors: Rossi
authors: Martina
authors: M
authors: Huber
authors: Edgar J J
authors: EJ
authors: Groenen