title: Simultaneous quantitation of indole 3-acetic Acid and abscisic Acid in small samples of plant tissue by gas chromatography/mass spectrometry/selected ion monitoring.
abstract: Indole 3-acetic acid (IAA) was analyzed in apple, orange, and prune tissue by GC-MS by monitoring the protonated molecular ion of its methyl ester at mass to charge ratio (m/z) 190 together with the major fragment ion at m/z 130 and the corresponding ions from the methyl esters of either [(2)H(4)]IAA (m/z 194, 134) or [(2)H(5)]IAA (m/z 195, 135). Abscisic acid (ABA) was analyzed by monitoring the major fragment ions of its methyl ester at m/z 261 and m/z 247 and the corresponding ions from the methyl ester of [(2)H(3)]ABA (m/z 264, 250). Detection limits for IAA and ABA were 1 and 10 picograms, respectively using standards and 1 nanogram per gram dry weight for both phytohormones in plant tissue.
authors: Department of Agronomy and Horticultural Science, University of Sydney, N.S.W. 2006, Australia.
authors: J H
authors: JH
authors: Vine
authors: D
authors: D
authors: Noiton
authors: J A
authors: JA
authors: Plummer
authors: C
authors: C
authors: Baleriola-Lucas
authors: M G
authors: MG
authors: Mullins