title: Comparison of glycerol, lactamide, acetamide and dimethylsulfoxide as cryoprotectants of Japanese white rabbit spermatozoa.
abstract: The rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese white rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 +/- 3.0%) was significantly (P<0.05) higher than in glycerol (17.0 +/- 3.3%). In addition, the rates of sperm plasma membrane integrity in lactamide and acetamide (35.9 +/- 3.3% and 30.2 +/- 3.0%, respectively) were significantly (P<0.05) higher than in glycerol (17.0 +/- 2.6%). The results indicate that 1.0 M lactamide and acetamide have higher cryoprotective effects than 1.0 M glycerol for cryopreservation of Japanese white rabbit spermatozoa.
authors: Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Japan. nkashi@azabu-u.ac.jp
authors: Naomi
authors: N
authors: Kashiwazaki
authors: Yasushi
authors: Y
authors: Okuda
authors: Yasunari
authors: Y
authors: Seita
authors: Shin
authors: S
authors: Hisamatsu
authors: Shigenori
authors: S
authors: Sonoki
authors: Masao
authors: M
authors: Shino
authors: Toshio
authors: T
authors: Masaoka
authors: Tomo
authors: T
authors: Inomata