title: Distribution of calmodulin-stimulated ca transport into membrane vesicles from green spinach leaves.
abstract: A microsomal fraction isolated from green spinach leaves exhibited a Mg(2+) and ATP-dependent (45)Ca(2+) uptake. Addition of 10 micromolar carbonyl cyanide m-chlorophenylhydrazine had no effect. The cationophore A23187 (10 micromolar) induced the release of (45)Ca(2+) accumulated by membrane vesicles. Membranes prepared from lower epidermis showed the highest Ca(2+) accumulation activity. Microsomal fractions from petiole, lamina, and midrib were less active. The stimulation by bovine brain calmodulin was about 30% for the lower epidermis, 23.5% for midrib, and below 20% for petiole and lamina.
authors: Laboratoire de Physiologie végétale, Université de Genève, 1211 Genève 4, Switzerland.
authors: V
authors: V
authors: Stosic
authors: C
authors: C
authors: Penel
authors: D
authors: D
authors: Marme
authors: H
authors: H
authors: Greppin