Purification of NADH-Nitrate Reductase by Affinity Chromatography.
ASSIMILATORY NITRATE REDUCTASE (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the presence of high ionic strength buffer but is rapidly eluted in the presence of mumolar concentrations of NADH.
Solomonson
L P
LP
Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242.