Optimization of enantioselective resolution of racemic ibuprofen by native lipase from Aspergillus niger.
Resolution of (R,S)-ibuprofen (2-(4-isobutylphenyl)propionic acid) enantiomers by esterification reaction with 1-propanol in different organic solvents was studied using native Aspergillus niger lipase. The main variables controlling the process (enzyme concentration and 1-propanol:ibuprofen molar ratio) have been optimized using response surface methodology based on a five-level, two-variable central composite rotatable design, in which the selected objective function was enantioselectivity. This enzyme preparation showed preferentially catalyzes the esterification of R(-)-ibuprofen, and under optimum conditions (7% w/v of enzyme and molar ratio of 2.41:1) the enantiomeric excess of active S(+)-ibuprofen and total conversion values were 79.1 and 48.0%, respectively, and the E-value was 32, after 168 h of reaction in isooctane.
de O Carvalho
Patrícia
P
Curso de Farmácia, Universidade São Francisco, Av. São Francisco de Assis, 218, 12916-900, Bragança Paulista, São Paulo, Brazil. patcarvalho@saofrancisco.edu.br
Contesini
Fabiano J
FJ
Bizaco
Renato
R
Calafatti
Silvana Ap
SA
Macedo
Gabriela A
GA