title: Expression of cab Genes in Douglas-Fir Is Not Strongly Regulated by Light.
abstract: Dark-grown Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) seedlings had approximately 30% of the major polypeptide of the light-harvesting chlorophyll a/b binding protein, 30% of cab mRNA, 54% of psbA mRNA, and 14% of total chlorophyll, in comparison with amounts in light-grown seedlings. Seedlings entrained under a 24-hour photoperiod of light and dark showed small diurnal fluctuations in cab and psbA mRNA levels and, when transferred to continuous conditions, no circadian rhythms in mRNA levels were apparent. These results suggest that regulation of cab gene expression in Douglas-fir differs from regulation in angiosperms, because in the latter, both light and circadian factors strongly influence the expression of cab genes.
authors: Institute of Forest Genetics, Pacific Southwest Forest and Range Experiment Station, Berkeley, California 94701.
authors: M C
authors: MC
authors: Alosi
authors: D B
authors: DB
authors: Neale
authors: C S
authors: CS
authors: Kinlaw