Co-infection of human parvovirus B19 in Vietnamese patients with hepatitis B virus infection.
OBJECTIVE
Human parvovirus B19 (B19) has been identified in the serum of hepatitis B virus (HBV) infected patients. However, the effect of B19-infection on the course of HBV-associated liver disease has not previously been investigated. We examined the prevalence of B19-DNA in HBV-infected Vietnamese patients and analysed the association between co-infection and the clinical outcome of HBV-infection.


METHODS
Serum samples from 399 HBV-infected patients and 64 healthy individuals were analysed for the presence of B19-DNA by PCR and DNA-sequencing.


RESULTS
B19-DNA was detected in 99/463 (21.4%) individuals. The proportion of HBV-infected patients who were also co-infected with B19 was higher than the healthy controls (P<0.001). B19-DNA was detected more frequently in patients with HBV-associated hepatocellular carcinoma compared to patients with acute and chronic HBV, HBV-associated liver cirrhosis and healthy subjects (P<0.006). A positive correlation was also found between B19-DNA loads and both serum HBV-DNA loads and alanine aminotransferase (rho>0.250 and P<0.05).


CONCLUSIONS
Our findings demonstrate that B19-infection is frequent in HBV-infected Vietnamese patients. Also, a significant correlation exists between HBV/B19 co-infection and a greater likelihood of progression to more severe hepatitis B-associated liver disease. Further studies are required to determine the role of B19-infection on HBV-associated pathogenesis.
Toan
Nguyen L
NL
Department of Molecular Pathology, Institute of Pathology, University Hospital of Tuebingen, Germany.
Song
Le H
le H
Kremsner
Peter G
PG
Duy
Dinh N
DN
Binh
Vu Q
VQ
Duechting
Anja
A
Kaiser
Heike
H
Torresi
Joseph
J
Kandolf
Reinhard
R
Bock
C-Thomas
CT