The activation of lytic replication of Epstein-Barr virus by baculovirus-mediated gene transduction.
A baculoviral mammalian-cell vector was constructed to express Rta, a protein of Epstein-Barr virus (EBV) responsible for the transition from latent infection to lytic replication. EBV lytic replication and cell-growth inhibition was observed in infected D98/HR1 cells. The baculovirus caused little cytotoxicity in the non-targeted HeLa cells, compared to an adenovirus vector. It is concluded that recombinant baculovirus might have the potential as a vector for the therapy of EBV-related cancer.
Wang
L
L
Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, P.R. China.
Shan
L
L
Yin
J
J
Zhao
M
M
Su
D
D
Zhong
J
J