One-flask synthesis of dinucleoside tetra- and pentaphosphates.
[reaction: see text] We report a one-flask route for the synthesis of dinucleoside tetra- and pentaphosphates, in isolated yields of 50-85%. This route relies on a mixture of P(III) and P(V) chemistries, using phosphitylation of a protected nucleoside with 2-chloro-4H-l,3,2-benzo-dioxaphosphorin-4-one (salicylchlorophosphite), followed by sequential reaction with inorganic pyrophosphate and a nucleoside 5' mono- or diphosphate.
Han
Qianwei
Q
Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, 08854, USA.
Gaffney
Barbara L
BL
Jones
Roger A
RA