title: Inositol-containing lipids in suspension-cultured plant cells: an isotopic study.
abstract: Polar lipids were extracted from suspension-cultured tomato (Lycopersicon esculentum Mill.) cells and analyzed by thin layer chromatography. Four major inositol-containing compounds were found, and incorporation of [(32)P]orthosphosphate, [2-(3)H]glycerol, and myo-[2-(3)H]inositol was studied. Results showed that phosphatidylinositol-monophosphate is the phospholipid in these cells displaying the most rapid incorporation of [(32)P]orthophosphate. We suggest that the tracer is incorporated primarily into the phosphomonoester group. Two inositol-containing lipids showed chromatographic behavior similar to phosphatidylinositol-4,5-bisphosphate when using standard thin layer chromatography techniques. The labeling pattern of these compounds, however, reveals that it is unlikely that either of these is identical to phosphatidylinositol-4,5-bisphosphate. Should phosphatidylinositol-bisphosphate be present in suspension cultured plant cells, our data indicate chemical abundancies substantially lower than previously reported.
authors: Department of Soil, Water and Plant Nutrition, Royal Veterinary- and Agricultural University, Thorvaldsensvej 40, 1871 Copenhagen C., Denmark.
authors: B K
authors: BK
authors: Drøbak
authors: I B
authors: IB
authors: Ferguson
authors: A P
authors: AP
authors: Dawson
authors: R F
authors: RF
authors: Irvine