Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection.
We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis of B. pertussis infection even in clinical laboratories with no specific equipment.
Kamachi
Kazunari
K
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Musashimurayma-city, Tokyo 208-0011, Japan. kamachi@nih.go.jp
Toyoizumi-Ajisaka
Hiromi
H
Toda
Kohei
K
Soeung
Sann Chan
SC
Sarath
Svay
S
Nareth
Ya
Y
Horiuchi
Yoshinobu
Y
Kojima
Kazunobu
K
Takahashi
Motohide
M
Arakawa
Yoshichika
Y