title: Water soluble nanoparticles from PEG-based cationic hyperbranched polymer and RNA that protect RNA from enzymatic degradation.
abstract: Recent advances in understanding biological systems have proven that RNA is not merely the carrier of genetic information, but also a key molecule in regulation of gene expression and other crucial metabolic processes. Therefore, it is being considered as an ideal therapeutic candidate both for metabolic and genetic disorders. However, research involving RNA molecules faces a practical limitation since RNA is highly labile. We have developed a novel method to protect RNA from cleavage by complexing it with a hyperbranched cationic polymer. It was found that total cellular RNA isolated from yeast spontaneously interacts with the positively charged polymer to form a spherical nanoparticle morphology. This interaction protects the RNA against enzymatic degradation. This methodology can be easily adapted for long-term storage of RNA, long distance transfer of RNA, and genetic engineering using RNA as a building block.
authors: Institute of Genomics and Integrative Biology, CSIR, Mall Road, Delhi 110 007, India.
authors: Jameel Ahmad
authors: JA
authors: Khan
authors: Rajesh Kumar
authors: RK
authors: Kainthan
authors: Munia
authors: M
authors: Ganguli
authors: Jayachandran N
authors: JN
authors: Kizhakkedathu
authors: Yogendra
authors: Y
authors: Singh
authors: Souvik
authors: S
authors: Maiti