Rapid development of polyclonal antisera against infectious salmon anaemia virus and its optimization and application as a diagnostic tool.
Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a drawback in its usefulness in other tests. This study describes the development and optimization of a polyclonal antiserum against infectious salmon anaemia virus, including a method of separating virus from cell culture components within culture supernatant. The antiserum was subsequently optimized for use in a variety of immunological diagnostic tests, including IFAT and an alkaline phosphatase-based immunoassay, and Western blot.
McBeath
S J
SJ
FRS Marine Laboratory, Aberdeen, UK.
Ellis
L M
LM
Cook
P F
PF
Wilson
L
L
Urquhart
K L
KL
Bricknell
I R
IR