Identification of a new target region on the long arm of chromosome 7 in gastric carcinoma by loss of heterozygosity.
OBJECTIVE
To define the common deleted region on the long arm of haman chromosome 7q linked to primary gastric carcinomas in Chinese by loss of heterozygosity (LOH) and its clinical significance.


METHODS
Nine microsatellite markers distributed over chromosome 7q with an average marker density of 10cM were used to examine 70 primary gastric carcinomas for LOH by PCR amplification. The PCR products were separated by electrophoresis on polyacrylamide gel. Genescan and Genotyper softwares were used to analyze LOH.


RESULTS
LOH with at least one marker on 7q occurred in 34.3% (12/50) of the tumors. Among them, LOH at D7S486 and D7S798 was higher in 24.0% (24/70) and 19.2% (5/26), respectively. By statistical analysis we also observed an obvious genotype-phenotype correlation on 7q (P<0.05). The frequency of LOH at D7S486 in patients with lymph node metastasis was significantly higher than that in those without lymph node metastasis (P=0.015).


CONCLUSIONS
The high incidence of LOH at D7S486 and its correlation with poorer prognosis suggest that there might be putative tumor suppressor genes in this region involved in the tumorigenesis and progression of gastric carcinoma.
Weng
De-Sheng
DS
Research Section of Oncology, State Key Laboratory of Oncology in Southern China, Cancer Center, Zhongshan University, Guangzhou 510060, Guangdong Province, China.
Li
Jin-Tian
JT
Mai
Shi-Juan
SJ
Pan
Zhi-Zhong
ZZ
Feng
Bing-Jian
BJ
Feng
Qi-Sheng
QS
Huang
Li-Xi
LX
Wang
Qi-Jing
QJ
Li
Yong-Qiang
YQ
Yu
Xing-Juan
XJ
Chen
Shi-Ping
SP
He
Jia
J
Xia
Jian-Chuan
JC