High-yielding enzymatic alpha-glucosylation of pyridoxine by marine alpha-glucosidase from Aplysia fasciata.
We recently succeeded in the identification and purification of an interesting marine exo-alpha-glucosidase (EC 3.2.1.20) from the anaspidean mollusc Aplysia fasciata. The enzyme was characterized by good transglycosylation activity toward different acceptors using maltose as donor. High-yielding enzymatic alpha-glycosylation of pyridoxine using this marine enzyme is reported here; the reaction has been optimized, reaching 80% molar yield of products (pyridoxine monoglucosides 24 g/l; pyridoxine isomaltoside 35 g/l). High selectivity toward the 5' position is observed for both monoglucoside and disaccharide formation. This is the first report describing the enzymatic production of pyridoxine isomaltoside.
Tramice
Annabella
A
Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Via Campi Flegrei 34, 80072 Pozzuoli, Naples, Italy.
Giordano
Assunta
A
Andreotti
Giuseppina
G
Mollo
Ernesto
E
Trincone
Antonio
A