Hypochlorite Disinfection Influences the GA(3)-Induced Synthesis of alpha-Amylase Isozymes by Isolated Barley Aleurone Layers.
Aleurone layers isolated from half-seeds of Himalaya barley (Hordeum vulgare cv Himalaya) disinfected in hypochlorite solutions containing 1.0% available chlorine synthesized significantly less alpha-amylase in response to gibberellic acid than layers derived from half-seeds disinfected in 0.1% hypochlorite. This effect of hypochlorite involved neither a differential decrease in the synthesis of group A or B alpha-amylase isozymes nor a general decrease in alpha-amylase synthesis attributable to fewer viable aleurone cells in layers from half-seeds disinfected with 1% hypochlorite. Our results emphasize the need to evaluate the potential effects of routine disinfection procedures used in physiological and biochemical studies.
Tittle
F T
FT
Department of Plant Science, University of Alberta, Edmonton, Alberta T6G 2P5 Canada.
Goudey
J S
JS
Spencer
M S
MS