title: NADH- and NAD(P)H-Nitrate Reductases in Rice Seedlings.
abstract: By use of affinity chromatography on blue dextran-Sepharose, two nitrate reductases from rice (Oryza sativa L.) seedlings, specifically, NADH:nitrate oxidoreductase (EC 1.6.6.1) and NAD(P)-H:nitrate oxidoreductase (EC 1.6.6.2), have been partially separated. Nitrate-induced seedlings contained more NADH-nitrate reductase than NAD(P)H-nitrate reductase, whereas chloramphenicol-induced seedlings contained primarily NAD(P)H-nitrate reductase. NAD(P)H-nitrate reductase was shown to utilize NADPH directly as reductant. This enzyme has a preference for NADPH, but reacts about half as well with NADH.
authors: Forschungsstelle Vennesland der Max-Planck-Gesellschaft, Harnackstrasse 23, 1 Berlin 33, Germany.
authors: T C
authors: TC
authors: Shen
authors: E A
authors: EA
authors: Funkhouser
authors: M G
authors: MG
authors: Guerrero