title: Influences of Helicobacter pylori on cyclooxygenase-2 expression and prostaglandinE2 synthesis in rat gastric epithelial cells in vitro.
abstract: OBJECTIVE
  It is known that cyclooxygenase (COX)-2 is over expressed in gastrointestinal neoplasia and Helicobacter pylori (H. pylori) infection is causally linked to gastric cancer. The present study aimed to elucidate the effects of H. pylori on COX-2 expression and prostaglandinE(2) (PGE(2)) production in a gastric epithelial cell line derived from normal rat gastric mucosa (RGM1).
  
  
  METHODS
  H. pylori water extracts were prepared from a supernatant of the H. pylori suspension in distilled water. RGM1 cells were cultured with H. pylori water extracts at the final concentration of 2.5, 5, 10 microg/mL for 24 h. For the time sequence study, RGM1 cells were cultured with 10 microg/mL H. pylori water extracts for 0, 6, 12, 24 and 48 h. COX-1 and COX-2 expression in the RGM1 cells was analyzed by western blotting. The levels of PGE(2) in the cultured media were measured by enzyme immunoassay.
  
  
  RESULTS
  H. pylori did not affect COX-1 expression; whereas COX-2 expression increased by six-fold at 24 h after incubation of RGM1 cells with 10 microg/mL H. pylori water extracts. The increase in COX-2 expression was evident after 12 h of incubation; reached a peak at 24 h and declined at 48 h. H. pylori dose dependently increased COX-2 expression and PGE(2) synthesis in RGM1 cells.
  
  
  CONCLUSIONS
  H. pylori induces COX-2 expression and increases PGE(2) synthesis in RGM1 cells in vitro. These results indicate that H. pylori-associated gastric carcinogenesis may depend on COX-2 expression.
authors: Department of Gastroenterology, Affiliated Hospital of Nanjing Traditional Chinese Medicine, Nanjing, China.
authors: Hong
authors: H
authors: Shen
authors: Wei-Hao
authors: WH
authors: Sun
authors: Qi-Ping
authors: QP
authors: Xue
authors: Jing
authors: J
authors: Wu
authors: Yun-Lin
authors: YL
authors: Cheng
authors: Guo-Xian
authors: GX
authors: Ding
authors: Hai-Ying
authors: HY
authors: Fu
authors: Shingo
authors: S
authors: Tsuji
authors: Sunao
authors: S
authors: Kawano