title: [Indirect ELISA for the pyrethroids-permethrin residue detection].
abstract: In order to detect the permethrin (Py) residue in environment samples, Ovalbumin(OVA)was used as protein carrier to couple with semi-antigen permethrin by active ester method. Indirect competitive ELISA (icELISA) was established. The most appropriate titration of coating antigen was 0.45 microg/mL and optimal dilution was 1/2 500 correspondingly. The standard curve of icELISA was also established and the curve indicated that the lowest detection limit was 0.l microg/mL, the curve had a favorable linear relation with the concentration range of 10 to approximately 800 microg/mL, recoveries of permethrin (>97%) were satisfactory.
authors: State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing 210093, China.
authors: Ting-feng
authors: TF
authors: Liu
authors: Ya-zi
authors: YZ
authors: Liu
authors: Cheng
authors: C
authors: Sun