title: Possible role of natural immune response against altered fibroblasts in the development of post-operative adhesions.
abstract: OBJECTIVE
  Post-operative adhesion tissue fibroblasts (ATF) differ from normal peritoneal fibroblasts (NPF). Natural immune response participates in the elimination of altered cells. In this study, we investigated NPF and ATF expression patterns of immune response-related markers, and lymphokine-activated killer (LAK) cell-mediated fibroblast elimination in vitro.
  
  
  METHODS
  Primary cell cultures of both NPF and ATF obtained from the same four patients were used in the experiments. The expression of CD54, CD40 and CD120b, and allogeneic LAK cell-mediated ATF and NPF elimination were studied by flow cytometry.
  
  
  RESULTS
  Average expression of CD54 in ATF was greater by 12.3-fold compared with NPF (P = 0.021), with ratios of 2.4 and 1.9-fold for CD40 (P < 0.001) and CD120b (P = 0.013), respectively. Average LAK cell-mediated fibroblast killing was 1.8 +/- 0.8-fold greater in ATF over NPF (P = 0.008). Furthermore, LAK cell-mediated fibroblast elimination correlated significantly with the increased CD40, CD54 and CD120b expression (R > 0.956; P < 0.05 for each).
  
  
  CONCLUSIONS
  These results demonstrate that ATF are more susceptible to lymphocyte-mediated elimination than NPF and the development of adhesions despite this could be explained by either impaired or overwhelmed autologous natural immune response against reactive fibroblasts.
authors: Hutzel Hospital, Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Wayne State University, Detroit, MI 48201, USA.
authors: Zeynep
authors: Z
authors: Alpay
authors: Melike S
authors: MS
authors: Ozgönenel
authors: Süreyya
authors: S
authors: Savaşan
authors: Steven
authors: S
authors: Buck
authors: Ghassan M
authors: GM
authors: Saed
authors: Michael P
authors: MP
authors: Diamond