Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts.
Several proteases, i.e., pronase, a mixture of trypsin and chymotrypsin, and thermolysin were screened as potential surface probes of isolated intact pea (Pisum sativum var Laxton's Progress No. 9) chloroplasts. Of these, only thermolysin met the criteria of a suitable probe. Thermolysin destroyed outer envelope polypeptides, but did not affect inner envelope polypeptides, envelope permeability properties or such chloroplast activities as metabolite transport and O(2) evolution.
Cline
K
K
Department of Botany, University of Wisconsin, Madison, Wisconsin 53706.
Werner-Washburne
M
M
Andrews
J
J
Keegstra
K
K