[Generation of transgenic mouse of dentin sialoprotein and transgene expression analysis].
OBJECTIVE
To generate the transgenic mouse model of DSP and perform transgene expression analysis by RT-PCR.


METHODS
Plasmid pcDNA3.1-CX was constructed by substituting promoter cbeta-actin for CMV promoter of pcDNA3.1, and the ultimate transgenic vector, pcDNA3.1-CX-dsp, was constructed by cloning DSP coding sequence into pcDNA3.1-CX. The pcDNA3.1-CX-dsp plasmid was linearized and microinjected into the male pronucleus of the zygotes. The tail DNA of pups was tested by PCR and Southern blot. A member of F1 generation of one positive mouse was used to perform transgene expression analysis by RT-PCR.


RESULTS
717 embryos were implanted to 29 recipient pseudopregnant mice, 4 of the 67 pups carrying the transgene. Expression of DSP was detected in a member of F1 generation of one positive mouse by RT-PCR.


CONCLUSIONS
Founders of the DSP transgenic mouse were obtained successfully, and the expression of DSP was primarily confirmed.
Sun
Han-tang
HT
Department of Oral Medicine, College of Stomatology, Fourth Military Medical University, Xi'an 710032, Shanxi Province, China. hantang@fmmu.edu.cn
Xiao
Ming-zhen
MZ
Wu
Bu-ling
BL
Zhu
Qing-ling
QL
Fei
Jian
J