title: Phytochrome destruction: an apparent requirement for protein synthesis in the induction of the destruction mechanism.
abstract: Examination of the phytochrome destruction reaction as a function of age in etiolated oat (Avena sativa L. cv. Garry) seedlings demonstrates that following illumination of 3-day-old shoots there is a lag, not observed in 4- or 5-day-old oats, prior to the onset of destruction. This light-mediated induction of the phytochrome destruction mechanism in 3-day-old shoots is inhibited by chloramphenicol, actinomycin D, and puromycin suggesting that protein synthesis is required. In 4-day-old shoots, actinomycin D and puromycin do not alter the kinetics of destruction while chloramphenicol partially inhibits the process. Thus, the inhibitors have a specific effect on the induction of the destruction mechanism but not its subsequent operation.
authors: Department of Biology, Vanderbilt University, Nashville, Tennessee 37235.
authors: G H
authors: GH
authors: Kidd
authors: L H
authors: LH
authors: Pratt