A New Sensitive Root Auxanometer: Preliminary Studies of the Interaction of Auxin and Acid pH in the Regulation of Intact Root Elongation.
A new sensitive root auxanometer is described. The auxanometer represents an adaption of the position-sensor transducer method to measurement of intact root elongation and has the advantages of simplicity and high sensitivity. Experiments with the auxanometer show that auxin begins to inhibit intact pea root elongation within 10 minutes and continues to inhibit elongation for at least 1 hour following a 1-hour treatment with the hormone. Exposure of pea roots to pH 4 results in a 2- to 3-fold increase in elongation rate beginning about 1 minute after acid treatment. Acid-induced elongation continues at a steady rate for at least 160 minutes and can be reinitiated repeatedly by shifting between pH 4 and 6.5. Auxin inhibits acid-induced elongation whether given before or after acidification, and a transient exposure to auxin renders intact roots relatively insensitive to acid for at least 1 hour after withdrawal of the hormone.
Evans
M L
ML
Department of Botany, The Ohio State University, Columbus, Ohio 43210.