Apolipoprotein A-I possessing activity of defensin proteins modifies structure of plasma membranes.
We studied the in vitro effect of apolipoprotein A-I possessing activity of defensin proteins on the structure of plasma membranes in donor erythrocytes and lymphocytes. Incubation of erythrocyte membranes with apolipoprotein A-I was accompanied by significant changes in biophysical characteristics of a fluorescent probe pyrene in the hydrophobic membrane region and a decrease in Na+/K(+)-ATPase activity. Microviscosity of annular lipids in the plasma membrane of lymphocytes increased during incubation with apolipoprotein A-I. Our results suggest that membranotropic activity of apolipoprotein A-I is associated with the presence of amphipathic alpha-helix regions.
Panin
L E
LE
Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk.
Ryazantseva
N V
NV
Novitskii
V V
VV
Tokareva
N V
NV