title: Cytokinins in Vegetative and Reproductive Buds of Pseudotsuga menziesii.
abstract: Immunoaffinity techniques using columns of immobilized antibodies raised against zeatin riboside and isopentenyladenosine were found to be effective in isolating cytoklnins from vegetative, female, and male buds of Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco). The purified cytokinins were separated by reverse phase high performance liquid chromatography and analyzed by radioimmunoassay. Confirmation of cytokinin identities was by gas chromatography-mass spectrometry. Immediately prior to bud burst, all bud types contained three major cytokinins: isopentenyladenosine, zeatin riboside, and a hexose conjugate of zeatin riboside (not zeatin riboside O-glucoside). Zeatin-type cytokinins were present in relatively high concentration in vegetative and female buds. In male buds, however, relatively high levels of isopentenyladenosine were found together with low levels of zeatin-type cytokinins.
authors: Department of Forest Science, Oregon State University, Corvallis, Oregon 97331.
authors: J W
authors: JW
authors: Morris
authors: P
authors: P
authors: Doumas
authors: R O
authors: RO
authors: Morris
authors: J B
authors: JB
authors: Zaerr