title: The Synthesis of Guanosine 5'-Diphosphate l-Fucose from Guanosine 5'-Diphosphate 3,5-d-[H]Mannose Catalyzed by an Enzyme Extract from Fruits of the Flax.
abstract: An enzyme system from fruits of the flax plant is described that catalyzes the synthesis of the sugar nucleotide guanosine 5'-diphosphate l-fucose from guanosine 5'-diphosphate d-mannose with the intermediate formation of guanosine 5'-diphosphate 4-keto-6-deoxy-d-mannose. Tritium from-[(3)H]H(2)O was incorporated into the l-fucose portion of the sugar nucleotide in the course of the reaction, and tritium at the 3,5-carbons of the d-mannose moiety of GDP-d-mannose was exchanged with protons in the medium. These results support a mechanism of synthesis analogous to that proposed for the formation of l-rhamnose and other 6-deoxy sugars.
authors: Department of Biochemistry, Ohio State University, Columbus, Ohio 43210.
authors: G A
authors: GA
authors: Barber