Separation and quantitation of polyamines in plant tissue by high performance liquid chromatography of their dansyl derivatives.
High performance liquid chromatography in combination with fluorescence spectrophotometry can be used to separate and quantitate polyamines (putrescine, cadaverine, spermidine, spermine), prepared as their dansyl derivatives, from plant tissue. The procedure gives sensitive and consistent results for polyamine determinations in plant tissue. In a standard mixture, the minimal detection level was less than 1 picomole of polyamines.
Smith
M A
MA
Section of Plant Biology, Cornell University, Ithaca, New York 14853.
Davies
P J
PJ