The detection of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans in tooth, tongue and buccal mucosa plaques in children, using immunoslot blot assay (IBA).
The present study was to investigate the distribution of typical periodontpathic bacteria (Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans) in tooth, tongue and buccal mucosa plaques in 3 to 17 Year old children. Clinical parameters (Rates of df, d, DMF, and D; plaque and gingival index) for each subject were determined prior to the collection of each site plaque. Three periodontopathic bacteria on each site samples were detected using IBA. The frequency of three bacteria for tooth plaque was higher than that for tongue or buccal mucosa plaque. The frequency of Porphyromonas gingivalis and Prevotella intermedia in supragingival plaques was significantly higher than that of corresponding ones in tongue or buccal mucosa plaques. The three bacteria also occurred more frequently in subjects aged between 10 and 14 years. Periodontopathic bacteria may be enhanced in circumpubertal children.
Tanaka
Shoji
S
Department of Diagnostic & Therapeutic Sciences, Meikai University School of Dentistry, Saitama, Japan.
Minami
Maki
M
Murakami
Yukio
Y
Ogiwara
Takako
T
Seto
Kazuhito
K
Shoji
Masao
M
Hirata
Atushi
A
Abe
Sinnosuke
S
Watanabe
Sigeru
S
Fujisawa
Seiichiro
S