title: Abscisic Acid Levels during Early Seed Development in Sechium edule Sw.
abstract: The time-course growth of single tissues in pollinated and unpollinated ovules of Sechium edule Sw. is described in relation to the endogenous levels of abscisic acid. Quantitation of abscisic acid (ABA) in the minute amounts of material obtained after ovule dissection has been performed by using a highly specific and sensitive solid-phase radioimmunoassay based on a monoclonal antibody raised against free (S)-ABA. While the absolute amount of ABA rises in both types of ovules, only in unpollinated ones does this leads to an increase in the hormone concentration. Infact in pollinated ovules the rapid growth following pollination prevents, through a dilution effect, the increase in ABA concentration. Growth patterns and endogenous ABA levels are similar for integuments and nucellus tissues either in pollinated or unpollinated ovules. It is suggested that the growth inhibition induced by the increase in ABA concentration after anthesis could be counteracted by the pollination triggered fast ovule growth.
authors: Dipartimento di Biologia delle Piante Agrarie, Università degli Studi di Pisa, Viale delle Piagge No. 23, 56124 Pisa, Italy.
authors: P
authors: P
authors: Vernieri
authors: P
authors: P
authors: Perata
authors: R
authors: R
authors: Lorenzi
authors: N
authors: N
authors: Ceccarelli