[Human embryonic esophagus squamous epithelial cells cultured through microscopic stripping technique and digestion in vitro].
OBJECTIVE
To discuss the application possibility of the microscopic stripping technique used in the primary culture of human embryonic esophagus squamous epithelial cells, and of the methods for the isolation, depuration and subculture of the esophagus epithelial cells in vitro.


METHODS
The squamous epithelial cells were obtained from the esophagus mucous membrane of the 20-week abortion fetus through the microscopic stripping technique, and were digested with trypsin. Then, the morphological, immunohistochemical observation and the growth curve of the isolated cells were studied.


RESULTS
The isolated cells were spherical in the cell suspension and spherical-like or polygon-like after attachment to the culture flask. The squamous epithelial specialized cytokeratin staining was strongly positive. And the morphological studies by the transmission electron microscopy indicated that the cultured cells were squamous epithelial cells. The squamous epithelial cells reached the peak level 3-4 days after the transfer of the culture. The absorbance at 3 and 4 days was significantly higher than that at 1, 2, 5 and 6 days (P < 0.05).


CONCLUSIONS
A large number of squamous epithelial cells can be available with the microscopic stripping technique and the digestion method. The cultured squamous epithelial cells can be proliferated quickly, and fit for the tissue engineering study.
Li
Wanli
W
Department of Orthopedics, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, PR China.
Cen
Shiqiang
S
Huang
Fuguo
F