title: [An unusual recombination event occurring between HLA-B and -Cw loci within a Chinese Han family].
abstract: OBJECTIVE
  To investigate the recombination event occurring between HLA-B and -Cw loci discovered in a family of Chinese Han nationality with an acute myeloid leukemia (AML) patient.
  
  
  METHODS
  Peripheral blood samples were collected from a Chinese man with M5 type AML, aged 39, and his healthy wife and daughter, all of Han nationality. HLA class I (-A, -B, and -Cw) and II (-DRB1 and -DQB1) alleles were typed by both low and high resolution PCR with sequence specific primers (PCR-SSP) and sequence-based typing (SBT). Then the recombination sites were analyzed by family study.
  
  
  RESULTS
  The 2 haplotypes of the patient, his daughter, and his wife were A*2402101-Cw*030401/0402-B*1301-DRB1*0406-DQR1*030302/0303 and A*02011-Cw*150201/0202-B*4002-DRB1*1405-DQB1*05031, A*02011-Cw*150201/0202-B*1301-DRB1*0406-DQB1*030302/0303 and A*2406-Cw*0602-B*1302_DRB1*070101/0102-DQB1*0202, and A*330301/0302-Cw*030201/0202-B*58-1-DRB1*17-DQB1*0202 and A*2406-Cw*0602-B*1302-DRB1*070101/0102-B*5801-DRB1*0202 respectively. Family study demonstrated that A*02011-Cw*150201/0202 recombination and B*1301-DRB1*030302/0303 recombination carried by the daughter came from the 2 isolated chromosomes of her father, indicating that the recombination event occurred between HLA-B and -Cw loci during meiosis of the father and resulted in a new HLA haplotype that was inherited by the daughter.
  
  
  CONCLUSIONS
  An unusual HLA-B/Cw recombination event occurring between HLA-B and -Cw loci has been found in a Han family, which helps further study the mechanisms of HLA recombination.
authors: Department of Immunology, Hospital Affiliated to the Academy of Military Medical Sciences, Laboratory of Immunoassay, National Center of Biomedical Analysis, Beijing 100071, China.
authors: Yuan
authors: Y
authors: Luo
authors: Yu-ying
authors: YY
authors: Sun
authors: Li
authors: L
authors: Jin
authors: Fei
authors: F
authors: Liang
authors: Nan
authors: N
authors: Liu
authors: Xin-qiang
authors: XQ
authors: Song
authors: Fang
authors: F
authors: Yuan
authors: Shu-guang
authors: SG
authors: Liu
authors: Jin-feng
authors: JF
authors: Liu
authors: Yong-zhi
authors: YZ
authors: Xi