title: [Detection and analysis of partial sequences isolated from human and swine in rural area of southern China].
abstract: OBJECTIVE
  To study the genotype of swines isolated from humans and their relationships with hepatitis E virus (HEV) in the rural areas of southern China.
  
  
  METHODS
  Specimens collected from normal people with HEV-IgM positive, acute hepatitis E patients and from swine in the same area were detected for HEV RNA using RT-nPCR with ORF2 primers. The positive PCR products were cloned and sequenced.
  
  
  RESULTS
  13 out of the 132 samples from swine stool, 4 of 26 HEV-IgM positive sera of normal people and 1 of 4 acute hepatitis E patients' stool sample and sera were tested positive for HEV RNA. Data from sequence analysis showed that the identity at nucleotide level was 89.3%-100.0% among the 10 isolates which shared 78.7% - 84.7%, 83.3% - 85.3%, 76.0% - 80.0% and 84.7% - 95.3% nucleotide sequence identity with HEV genotype I, II, III and IV respectively in the region (nt6317- 6466).
  
  
  CONCLUSIONS
  HEV circulating in humans and swine in the area belonged to genotype IV.
authors: Department of Epidemiology, School of Public Health, Central South University, Changsha 410078, China.
authors: Xiao
authors: X
authors: Zhang
authors: Ying-Jie
authors: YJ
authors: Zheng
authors: Fa-Di
authors: FD
authors: Wang
authors: Mei-Yang
authors: MY
authors: Gao
authors: Jian-Fu
authors: JF
authors: Zhu
authors: Qing-Wu
authors: QW
authors: Jiang