title: Identification of novel HrpXo regulons preceded by two cis-acting elements, a plant-inducible promoter box and a -10 box-like sequence, from the genome database of Xanthomonas oryzae pv. oryzae.
abstract: A regulatory protein HrpXo of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, is known to control the expression of hrp genes that encode components of a type III secretion system and of some effector protein genes. In this study, we screened novel HrpXo regulons from the genome database of X. oryzae pv. oryzae, searching for ORFs preceded by two predicted sequence motifs, a plant-inducible promoter box-like sequence and a -10 box-like sequence. Using a gus reporter system, nine of 15 ORF candidates were expressed HrpXo dependently. We also showed by base-substituted mutagenesis that both motifs are essential for the expression of the genes.
authors: Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Prefectural University, Kyoto, Japan.
authors: Ayako
authors: A
authors: Furutani
authors: Takeshi
authors: T
authors: Nakayama
authors: Hirokazu
authors: H
authors: Ochiai
authors: Hisatoshi
authors: H
authors: Kaku
authors: Yasuyuki
authors: Y
authors: Kubo
authors: Seiji
authors: S
authors: Tsuge