title: Involvement of CCL18 in allergic asthma.
abstract: Allergic asthma is associated with a pulmonary recruitment of Th type 2 cells, basophils, and eosinophils, mainly linked to chemokine production. CCL18 is a chemokine preferentially expressed in the lung, secreted by APCs, induced by Th2-type cytokines, and only present in humans. Therefore, CCL18 may be involved in allergic asthma. PBMC from asthmatics allergic to house dust mite cultured in the presence of Dermatophagoides pteronyssinus 1 (Der p 1) allergen secreted CCL18, 48 and 72 h after stimulation, whereas those from healthy donors did not. Part of CCL18 was directly derived from Der p 1-stimulated plasmacytoid dendritic cells, whereas the other part was linked to monocyte activation by IL-4 and IL-13 produced by Der p 1-stimulated T cells. In bronchoalveolar lavages from untreated asthmatic allergic patients, CCL18 was highly increased compared with controls. Functionally, CCL18 preferentially attracted in vitro-polarized Th2 cells and basophils, but not eosinophils and Th1 cells, and induced basophil histamine and intracellular calcium release. These data show a new function for CCL18, i.e., the recruitment of Th2 cells and basophils, and suggest that CCL18 may play a predominant role in allergic asthma.
authors: Institut National de la Sante et de la Recherche Medicale U-774, Institut Pasteur de Lille, 59019 Lille Cedex, France.
authors: Patricia
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authors: de Nadaï
authors: Anne-Sophie
authors: AS
authors: Charbonnier
authors: Cécile
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authors: Chenivesse
authors: Stéphanie
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authors: Sénéchal
authors: Clément
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authors: Fournier
authors: Jules
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authors: Gilet
authors: Han
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authors: Vorng
authors: Ying
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authors: Chang
authors: Philippe
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authors: Gosset
authors: Benoît
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authors: Wallaert
authors: André-Bernard
authors: AB
authors: Tonnel
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authors: Lassalle
authors: Anne
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