title: [Cell penetrating peptides enhance intracellular translocation and function of siRNA encapsulated in Pegylated liposomes].
abstract: OBJECTIVE
  To prepare the PEGylated liposomes modified with cell penetrating peptides, which protect siRNA from nuclease degradation and deliver efficiently siRNA into cells to facilitate silencing of target gene.
  
  
  METHODS
  The purity of R8-PEG-PE and pNP-PEG-PE was detected by HPLC; the quantity of R8, PEG-DPPE modified R8, and R8 attached to the out membrane surface of the liposomal siRNA by transfer from R8-PEG-DPPE micelles to the liposomes was tested by fluorescence; Size and size distribution of siRNA loaded liposomes with and without attached R8 were determined by Zetasizer 5000; A comparison of mediated siRNA transfection efficiency between R8-liposomes and lipofectamine 2000 was examined by individual inside cell fluorescence intensity; The growth inhibition of small cell lung carcinoma NCI-H446 cells treated with R8-liposomal hdm2-siRNA or lipofectamine 2000-hdm2-siRNA complex was tested by MTT assay.
  
  
  RESULTS
  The retention times of PEG-DPPE and R8-PEG-DPPE were 9.0 min and 7.8 min, respectively. Fluorescence scanning indicated that lipids composed of liposomes and siRNAs didn't interfere to the determination of R8 when it was attached to the liposomal siRNA. The cells treated with R8-liposomal hdm2-siRNA significantly enhanced the cellular uptake of hdm2-siRNA and facilitated the functions of hdm2-siRNA through silencing of target gene which, in turn, inhibited tumor cell growth, compared with lipofectamine 2000.
  
  
  CONCLUSIONS
  The R8 attached liposomes are shown to be powerful carriers for delivery siRNAs into cell to silence targeted gene.
authors: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors: Wei
authors: W
authors: Wang
authors: Ning
authors: N
authors: Tang
authors: Chun-ling
authors: CL
authors: Zhang
authors: Xing-jun
authors: XJ
authors: Liu
authors: Han
authors: H
authors: Hu
authors: Zhi-xiang
authors: ZX
authors: Zhang
authors: Wei
authors: W
authors: Liang