Identification of isocephalomannine in the presence of cephalomannine isomers and alkali metal ion adducts in a paclitaxel active pharmaceutical ingredient using electrospray tandem mass spectrometry.
A strategy is developed for the identification of isocephalomannine in the presence of alkali metal ion adducts and other cephalomannine isomers in a paclitaxel active pharmaceutical ingredient. Intact molecular ion analyses and a sub-structural study have been performed for the differentiation of isocephalomannine (2-debenzoylpaclitaxel-2-pentenoate) from cephalomannine and 7-epi-cephalomannine. A comparative study of the cephalomannine isomers was carried out using molecular ions (MS) and fragmentation patterns (MS/MS) for sub-structural analysis. An attempt has been made to identify isocephalomannine in Cremophor(R) EL formulations.
Vivekanandan
Kannan
K
Mass Spectrometry Laboratory, Dabur Research Foundation, 22 Site IV, Sahibabad, UP, 201010, India. vivekanandank@dabur.com
Swamy
Madugundu Guru
MG
Prasad
Sudhanand
S
Mukherjee
Rama
R
Burman
Anand C
AC