title: High performance liquid chromatography of the dansyl derivatives of putrescine, spermidine, and spermine.
abstract: A high performance liquid chromatographic (HPLC) method, based on dansylation and fluorescence detection, is described for the estimation of putrescine, spermidine, and spermine in lichen (Evernia prunastri [L.]) samples. Because of the high concentrations of phenols and salts, dansylation was followed by a pre-HPLC purification step. Both flow rate and mobile phase (methanol:water) followed a gradient for optimum resolution on a reverse-phase column. Amounts as small as 0.3 picomole of standard polyamines could be detected. In applying the method to lichens, it was found that 5.45% (w/w) of the exogenous putrescine taken up by the thallus was unbound in the algal partner and that 60% (w/w) was conjugated in the thallus, perhaps to lichen phenolics.
authors: Laboratory of Plant Physiology, Department of Biology, Complutense University of Madrid, 28040 Madrid, Spain.
authors: M I
authors: MI
authors: Escribano
authors: M E
authors: ME
authors: Legaz