title: Preventing photochemistry in culture media by long-pass light filters alters growth of cultured tissues.
abstract: Exposure of plant tissue culture media to light from fluorescent bulbs changed the growth regulating properties of the media. The light caused nutrient medium-dependent photosensitized degradation of the phytohormone indole-3-acetic acid and other media components. Photochemical changes in culture media were caused by light from 290 to 450 nanometers and were prevented with a yellow long-pass filter. The use of appropriately filtered light when culturing plant material can eliminate unnecessary variability by stabilizing the culture media composition.
authors: Department of Botany, Ohio State University, Columbus, Ohio 43210.
authors: T C
authors: TC
authors: Stasinopoulos
authors: R P
authors: RP
authors: Hangarter