title: Interleukin-8 expression by mammary gland endothelial and epithelial cells following experimental mastitis infection with E. coli.
abstract: Epithelial and endothelial cells play a pivotal role in initiating and controlling the movement of leukocytes into tissues during inflammation through the production of cytokines and chemokines such as interleukin-8 (IL-8). In situ hybridization with an IL-8 riboprobe was used to determine IL-8 mRNA expression by mammary gland epithelial and endothelial cells in cows with experimental Escherichia coli mastitis. Epithelial cells of the gland, especially surrounding the alveoli, had increased IL-8 mRNA levels at all time points at which tissue samples were collected (8, 12, and 24h) after E. coli challenge. Levels of IL-8 expression in the epithelial cells decreased at 24h post-infection. IL-8 expression by mammary gland endothelial cells was low, but did increase slightly at 24h post-infection. Both epithelial and endothelial cells of the mammary gland can contribute to the production of IL-8 that is typically seen in coliform mastitis.
authors: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA. mcclenahan@svm.vetmed.wise.edu
authors: David
authors: D
authors: McClenahan
authors: Rebecca
authors: R
authors: Krueger
authors: Haa-Yung
authors: HY
authors: Lee
authors: Chet
authors: C
authors: Thomas
authors: Marcus E
authors: ME
authors: Kehrli
authors: Charles
authors: C
authors: Czuprynski