title: Subcellular Distribution of O-Acetylserine(thiol)lyase in Cauliflower (Brassica oleracea L.) Inflorescence.
abstract: The subcellular localization of O-acetyiserine(thiol)lyase (EC 4.2.99.8) in nongreen tissue from higher plants has been studied using purified proplastids, mitochondria, and protoplasts from cauliflower (Brassica oleracea L.) buds as a source of subcellular fractions. O-Acetylserine(thiol)lyase has been detected in both organelles (proplastids and mitochondria) and a cytosolic extract obtained by protoplast fractionation. We confirmed these observations, demonstrating that a form of the enzyme different in global charge and separated from others by anion-exchange chromatography corresponded to each subcellular location. Our observations are consistent with the need for cysteine biosynthesis in each subcellular compartment where the synthesis of proteins occurs.
authors: Unité Mixte Centre National de la Recherche Scientifique/Rhône-Poulenc (Unité Associée au Centre National de la Recherche Scientifique, U.M. 41), Rhône-Poulenc Agrochimie, 14-20 impasse Pierre Baizet, 69263 Lyon Cedex 09, France.
authors: N
authors: N
authors: Rolland
authors: M
authors: M
authors: Droux
authors: R
authors: R
authors: Douce