UV embossed polymeric chip for protein separation and identification based on capillary isoelectric focusing and MALDI-TOF-MS.
This paper demonstrates a ultraviolet (UV)-embossed polymeric chip for protein separation by capillary isoelectric focusing (CIEF) and identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The polymeric chip was replicated by a UV-embossing technique using a soft rubber mold. Five diverse widely investigated families of UV-cured formulations were examined for MALDI ionization efficiency of bovine serum albumin (BSA) samples spotted on the polymer surfaces. The signal and signal-to-noise ratio from the polyester formulation were each 12 times those obtained with PMMA (a commonly used polymer material for MALDI) at picomole sample concentration. A polyester chip was successfully used to carry out CIEF to separate proteins, followed by MALDI-TOF-MS identification. Issues related to the successful chip fabrication and protein separation and identification are discussed.
Guo
Xun
X
Singapore-MIT Alliance, N2-B2c-15, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.
Chan-Park
Mary B
MB
Yoon
Soon Fatt
SF
Chun
Jung-hoon
JH
Hua
Lin
L
Sze
Newman S-K
NS