title: Role of Glutamate-oxaloacetate Transaminase and Malate Dehydrogenase in the Regeneration of NAD for Glycine Oxidation by Spinach leaf Mitochondria.
abstract: During glycine oxidation by spinach leaf mitochondria, oxygen consumption showed a strong and transient inhibition upon addition of oxaloacetate or aspartate plus alpha-ketoglutarate. During the course of the inhibition, aspartate and alpha-ketoglutarate were stoichiometrically transformed into malate and glutamate.It is concluded that oxaloacetate formed by transamination is reduced by the malate dehydrogenase, which allows the regeneration of NAD(+) for glycine oxidation and, thus, by-passes the respiratory chain. Efficiency of a malate-glutamate/aspartate-alpha-ketoglutarate shuttle upon illumination and under in vivo conditions is discussed.
authors: Physiologie Cellulaire Végétale, Département de Recherche Fondamentale/Biologie Végétale, Centre d'Etudes Nucléaires et Université Scientifique et Médicale de Grenoble, 85X 38041 Grenoble Cedex, France.
authors: E P
authors: EP
authors: Journet
authors: M
authors: M
authors: Neuburger
authors: R
authors: R
authors: Douce