title: [Study on the regular pattern of the distribution of skin epidermal stem cells in the different parts of a healthy human body].
abstract: OBJECTIVE
  To investigate the regular pattern of the distribution of skin epidermal stem cells (ESCs) in the different parts of a healthy human body, and to evaluate the feasibility of the identification of ESCs by P63 and CD29 with single and double labeling.
  
  
  METHODS
  Full-thickness skin samples from 21 parts (including scalp, dorsum of foot, sole of foot, pubic region, and scrotum) of 5 healthy persons were harvested for the study. Immunohistochemistry method with biotin-streptavidin-horseradish peroxidase (SP) was employed with P63 and CD29 as the first antibody to carry out single and double labeling. The staining results were subjected to image analysis. The distribution of the ESCs in the skin from the above parts was observed and expressed as positive unit (PU) value.
  
  
  RESULTS
  It was found by P63 single labeling and P63 and CD29 double labeling that the PU value in the dorsum of foot was the lowest while that in the scalp was the highest among all the parts of a healthy body. It was also found by CD29 single labeling that the PU value in the dorsum of foot was the lowest [(11.9 +/- 1.5)%] while highest in the scalp [(29.1 +/- 5.0)%]. The PU value in the hairy region of a human body was evidently higher than that in the non-hairy region (P < 0.01), when examined by P63 and CD29 single and double labeling. But there was no difference in the PU values between the trunk and limbs by means of P63 and CD29 single and double labeling (P > 0.05).
  
  
  CONCLUSIONS
  There are more ESCs in the skin from the scalp, mons pubis and scrotum than other parts of the body. Single P63 or CD29 labeling exhibits higher sensitivity but lower specificity in the identification of ESCs. While the double labeling method exhibits higher specificity but lower sensitivity. Above all, it seems that the double labeling may be a simple and effective method for the identification of ESCs.
authors: Department of Burns, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, PR China.
authors: Xiao-dong
authors: XD
authors: Chen
authors: Tian-zeng
authors: TZ
authors: Li
authors: Shao-hai
authors: SH
authors: Qi
authors: Ju-lin
authors: JL
authors: Xie
authors: Ying-bin
authors: YB
authors: Xu
authors: Shu
authors: S
authors: Pan
authors: Ji-Shan
authors: JS
authors: Yuan
authors: Tao
authors: T
authors: Zhang
authors: Hui-zhen
authors: HZ
authors: Liang