title: Polyphosphoinositides are present in plasma membranes isolated from fusogenic carrot cells.
abstract: Fusogenic carrot cells grown in suspension culture were labeled 12 hours with myo-[2-(3)H]inositol. Plasma membranes were isolated from the prelabeled fusogenic carrot cells by both aqueous polymer two-phase partitioning and Renografin density gradients. With both methods, the plasma membrane-enriched fractions, as identified by marker enzymes, were enriched in [(3)H]inositol-labeled phosphatidylinositol monophosphate (PIP) and phosphatidylinositol bisphosphate (PIP(2)). An additional [(3)H]inositol-labeled lipid, lysophosphatidylinositol monophosphate, which migrated between PIP and PIP(2) on thin layer plates, was found primarily in the plasma membrane-rich fraction of the fusogenic cells. This was in contrast to lysophosphatidylinositol which is found primarily in the lower phase, microsomal/mitochrondrial-rich fraction.
authors: Department of Botany, North Carolina State University, Raleigh, North Carolina 27695.
authors: J J
authors: JJ
authors: Wheeler
authors: W F
authors: WF
authors: Boss