[Study on hepatitis C virus genotyping in Yixing area, Jiangsu province].
OBJECTIVE
To investigate the distribution of hepatitis C virus (HCV) genotypes in Yixing, Jiangsu province.


METHODS
Genotypes identification on sera samples were obtained from 158 donors who had already been anti-HCV positive through PCR method with type specific primer designed according to the sequence of 5'non-coding region (5'NCR). 5'NCR was also sequenced and compared with published date. Genotypes distribution was investigated in patients with different sex and clinical types of hepatitis C.


RESULTS
Of the total 158 patients, 95 were HCV RNA positive in which 80 patients having genotype 1b (80/95; 84.4%), 5 patients having genotype 2(5/95; 5.3%), 5 patients with 1b/2 mixed genotypes (5/ 95; 5.3%) and another 5 patients whose genotype undetermined. The difference on the distribution of HCV genotypes was significant between female and male patients (P < 0.05) but not in different kinds of hepatitis C patients.


CONCLUSIONS
Type 1b was the predominant HCV genotype in Yixing area.
Xu
Ke
K
Department of Epidemiology, Nanjing Medical University, Nanjing 210029, China.
Deng
Xiao-zhao
XZ
Ding
Wei-liang
WL
Gao
Jian
J
Yu
Rong-bin
RB
Diao
Zhen-yu
ZY
Tan
Yong-fei
YF
Zhang
Yun
Y