title: Reactionary dentinogenesis after applying restorative materials and bioactive dentin matrix molecules as liners in deep cavities prepared in nonhuman primate teeth.
abstract: OBJECTIVE
  The aim of this in vivo study was to evaluate the response of the pulp-dentin complex following application of resin-modified glass-ionomer cement, calcium hydroxide hard-setting cement and EDTA-soluble preparation of dentine matrix proteins (ESDP) in deep cavities prepared in non-human primate teeth.
  
  
  METHODS
  Eighteen deep Class V buccal cavities were prepared in premolars of four capuccin monkeys. In Groups 1 and 2, the cavity floor was lined with ESDP or a resin-modified glass-ionomer cement (Vitrebond - 3M ESPE), respectively. In Group 3 (control), the cavity was lined with a hard setting calcium hydroxide cement (Dycal - Dentsply). The cavities were subsequently filled with amalgam. After 6 months, the animals were sacrificed and the teeth were prepared for microscopic assessment. Six-micron thick serial sections were stained with H/E, Masson's trichrome and Brown & Brenn techniques.
  
  
  RESULTS
  No inflammatory pulpal response was observed for all experimental and control Groups. However, the amount of reactionary dentin deposition differed between groups in the rank order ESDP (Group 1) > calcium hydroxide (Group 3) > resin-modified glass-ionomer (Group 2). These differences were statistically significant.
  
  
  CONCLUSIONS
  All materials were biocompatible when applied in deep cavities. ESDP stimulated higher deposition of reactionary dentin matrix than Vitrebond and Dycal.
authors: Department of Orthodontics and Pediatric Dentistry, School of Dentistry, Araraquara, University of Sao Paulo State, Sao Paulo, Brazil.
authors: C
authors: C
authors: Duque
authors: J
authors: J
authors: Hebling
authors: A J
authors: AJ
authors: Smith
authors: E M A
authors: EM
authors: Giro
authors: M F
authors: MF
authors: Oliveira
authors: C A
authors: CA
authors: de Souza Costa