title: Intracellular Localization of the Neurotoxin 2,4-Diaminobutyric Acid in Lathyrus sylvestris L. Leaf Tissue.
abstract: The intracellular distribution of the neurotoxin 2,4-diaminobutyric acid (DABA) in mature leaves of the perennial legume Lathyrus sylvestris L. var ;Lathco' (flatpea) was determined using subcellular fractions from mesophyll protoplasts. Chloroplasts contained about 15% of the cellular DABA. At least 75% of the DABA was vacuolar, based on the assumptions that each protoplast contained a single vacuole and that acid phosphatase occurred exclusively in the vacuole. DABA was not detectable in peroxisomal and mitochondrial fractions. Because the vacuole is not a major site of amino acid synthesis, this distribution implicates synthesis of DABA within chloroplasts with subsequent transport to and storage within the vacuoles of the mesophyll cells.
authors: United States Department of Agriculture, Agricultural Research Service, Appalachian Soil and Water Conservation Research Laboratory, P. O. Box 867, Airport Road, Beckley, West Virginia 25802-0867.
authors: J G
authors: JG
authors: Foster
authors: W D
authors: WD
authors: Cress
authors: S F
authors: SF
authors: Wright
authors: J L
authors: JL
authors: Hess