Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay.
The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information.
Francois
Patrice
P
Genomic Research Laboratory, University of Geneva Hospitals, Service of Infectious Diseases/Genomic Research Laboratory, CH-1211 Geneva 14, Switzerland. patrice.francois@genomic.ch
Koessler
Thibaud
T
Huyghe
Antoine
A
Harbarth
Stephan
S
Bento
Manuela
M
Lew
Daniel
D
Etienne
Jérôme
J
Pittet
Didier
D
Schrenzel
Jacques
J