Regulation by Lipids of Plant Microsomal Enzymes: II. LIPID DEPENDENCE OF THE NADH-CYTOCHROME c REDUCTASE OF POTATO TUBERS.
Microsomal membranes from potato tubers were treated with a phospholipase C extracted from Bacillus cereus. A positive correlation could be observed between the hydrolysis of membranous phospholipids and the decrease of the NADH-cytochrome c reductase activity. Addition of total lipid or phospholipid micelles to phospholipase C-treated microsomes partially restored the NADH-cytochrome c reductase activity, thus proving the lipid-dependence of this enzyme.
Jolliot
A
A
Laboratoire de Physiologie Cellulaire, ERA 323, Université Pierre et Marie Curie, Tour 53, 4 place Jussieu, 75230 Paris Cédex 05, France.
Demandre
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C
Mazliak
P
P