[Effects of ADAM28 AS-ODN on proliferation and AKPase of HDFCs].
OBJECTIVE
To study the effects of ADAM28 antisense oligodeoxynucleotide (AS-ODN) on proliferation of human dental follicle cells (HDFCs) and activity of alkaline phosphatase (AKPase).


METHODS
Cell culture, gene transfection, MTT chromatometry and enzyme kinetics methods were used to detect the possible mechanisms of ADAM28 AS-ODN on proliferation, differentiation of HDFCs and effect of the activity of AKPase. Statistical significance was assessed by multiple comparison (q test, SNK) in one-way analysis of variance (ANOVA).


RESULTS
ADAM28 AS-ODN was transfected into HDFCs successfully. MTT and AKPase activity assays indicated that cell proliferation and AKPase activity of ADAM28 AS-ODN group were significantly lower than those of S-ODN and vacuity control group (P<0.01). MTT assay revealed P value between AS-ODN and S-ODN, blank control group was 0.0002, 0.0001 respectively, P<0.01; AKPase assay showed P value between AS-ODN and S-ODN, vacuity control group was 0.0007, 0.0003 respectively, P<0.01. ADAM28 AS-ODN could inhibit the proliferation and AKPase activity of HDFCs significantly.


CONCLUSIONS
ADAM28 may promote proliferation and differentiation of HDFCs by participating in Notch signal pathway.
Zhao
Zheng
Z
Department of Pediatric Dentistry, College of Stomatology, Fourth Military Medical University, Xi'an 710032, Shanxi Province, China. zz1700@fmmu.edu.cn
Wen
Ling-ying
LY
Jin
Yan
Y
Jin
Ming
M