title: Atelectasis causes alveolar injury in nonatelectatic lung regions.
abstract: BACKGROUND
  Many authors have suggested that the mechanism by which atelectasis contributes to injury is through the repetitive opening and closing of distal airways in lung regions that are atelectatic. However, neither the topographic nor mechanistic relationships between atelectasis and distribution of lung injury are known.
  
  
  OBJECTIVE
  To investigate how atelectasis contributes to ventilator-induced lung injury.
  
  
  METHODS
  Surfactant depletion was performed in anesthetized rats that were then allocated to noninjurious or injurious ventilation for 90 min.
  
  
  METHODS
  Lung injury was quantified by gas exchange, compliance, histology, wet-to-dry weight, and cytokine expression, and its distribution by histology, stereology, cytokine mRNA expression, in situ hybridization, and immunohistochemistry. Functional residual capacity, percent atelectasis, and injury-induced lung water accumulation were measured using gravimetric and volumetric techniques.
  
  
  RESULTS
  Atelectasis occurred in the dependent lung regions. Injurious ventilation was associated with alveolar and distal airway injury, while noninjurious ventilation was not. With injurious ventilation, alveolar injury (i.e., histology, myeloperoxidase protein expression, quantification, and localization of cytokine mRNA expression) was maximal in nondependent regions, whereas distal airway injury was equivalent in atelectatic and nonatelectatic regions.
  
  
  CONCLUSIONS
  These data support the notion that lung injury associated with atelectasis involves trauma to the distal airways. We provide topographic and biochemical evidence that such distal airway injury is not localized solely to atelectatic areas, but is instead generalized in both atelectatic and nonatelectatic lung regions. In contrast, alveolar injury associated with atelectasis does not occur in those areas that are atelectatic but occurs instead in remote nonatelectatic alveoli.
authors: Lung Biology Program, and Department of Critical Care Medicine, Hospital for Sick Children, Toronto, Ontario, Canada.
authors: Shinya
authors: S
authors: Tsuchida
authors: Doreen
authors: D
authors: Engelberts
authors: Vanya
authors: V
authors: Peltekova
authors: Natalie
authors: N
authors: Hopkins
authors: Helena
authors: H
authors: Frndova
authors: Paul
authors: P
authors: Babyn
authors: Colin
authors: C
authors: McKerlie
authors: Martin
authors: M
authors: Post
authors: Paul
authors: P
authors: McLoughlin
authors: Brian P
authors: BP
authors: Kavanagh