title: Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability.
abstract: Caveolin-1 is the major component protein of caveolae and associated with a lot of cellular events such as endocytosis, cholesterol homeostasis, signal transduction, and tumorigenesis. The majority of results suggest that caveolin-1 might not only act as a tumor suppressor gene but also a promoting metastasis gene. In this study, the divergent expression and roles of caveolin-1 were investigated in mouse hepatocarcinoma cell lines Hca-F, Hca-P, and Hepa1-6, which have high, low, and no metastatic potential in the lymph nodes, as compared with normal mouse liver cell line IAR-20. The results showed that expression of caveolin-1 mRNA and protein along with the amount of caveolae number in Hca-F cells was higher than that in Hca-P cells, but was not detectable in Hepa1-6 cells. When caveolin-1 expression in Hca-F cells was down-regulated by RNAi approach, Hca-F cells proliferation rate in vitro declined and the expression of lymphangiogenic factor VEGFA in Hca-F decreased as well. Furthermore, in vivo implantation assay indicated that reduction of caveolin-1 expression in Hca-F prevented the lymphatic metastasis tumor burden of Hca-F cells in 615 mice. These results suggest that caveolin-1 facilities the lymphatic metastasis ability of mouse hepatocarcinoma cells via regulation tumor cell growth and VEGFA expression.
authors: Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian 116027, China.
authors: Huimin
authors: H
authors: Zhou
authors: Li
authors: L
authors: Jia
authors: Shujing
authors: S
authors: Wang
authors: Hongmei
authors: H
authors: Wang
authors: Haiying
authors: H
authors: Chu
authors: Yichuan
authors: Y
authors: Hu
authors: Jun
authors: J
authors: Cao
authors: Jianing
authors: J
authors: Zhang