title: Autogenous bone graft with or without a calcium sulfate barrier in the treatment of Class II furcation defects: a histologic and histometric study in dogs.
abstract: BACKGROUND
  The purpose of this study was to histologically evaluate the healing of surgically created Class II furcation defects treated using an autogenous bone (AB) graft with or without a calcium sulfate (CS) barrier.
  
  
  METHODS
  The second, third, and fourth mandibular premolars (P2, P3, and P4) of six mongrel dogs were used in this study. Class II furcation defects (5 mm in height x 2 mm in depth) were surgically created and immediately treated. Teeth were randomly divided into three groups: group C (control), in which the defect was filled with blood clot; group AB, in which the defect was filled with AB graft; and group AB/CS, in which the defect was filled with AB graft and covered by a CS barrier. Flaps were repositioned to cover all defects. The animals were euthanized 90 days post-surgery. Mesio-distal serial sections were obtained and stained with either hematoxylin and eosin or Masson's trichrome. Histometric, using image-analysis software, and histologic analyses were performed. Linear and area measurements of periodontal healing were evaluated and calculated as a percentage of the original defect. Percentage data were transformed into arccosine for statistical analysis (analysis of variance; P <0.05).
  
  
  RESULTS
  Periodontal regeneration in the three groups was similar. Regeneration of bone and connective tissue in the furcation defects was incomplete in most of the specimens. Statistically significant differences were not found in any of the evaluated parameters among the groups.
  
  
  CONCLUSIONS
  Periodontal healing was similar using surgical debridement alone, AB graft, or AB graft with a CS barrier in the treatment of Class II furcation defects.
authors: Department of Surgery and Integrated Clinic, Dental School of Araçatuba, University of the State of São Paulo "Júlio de Mesquita Filho," Araçatuba, São Paulo, Brazil.
authors: Tatiana M
authors: TM
authors: Deliberador
authors: Maria J H
authors: MJ
authors: Nagata
authors: Flávia A C
authors: FA
authors: Furlaneto
authors: Luiz G N
authors: LG
authors: Melo
authors: Tetuo
authors: T
authors: Okamoto
authors: Maria L M M
authors: ML
authors: Sundefeld
authors: Stephen E
authors: SE
authors: Fucini