Salt-soluble lectins of corn grain.
Lectins extracted from corn (Zea mays L.) kernel with Tris-HCl buffer pH 7.5 were isolated from the crude extract by affinity chromatography on Sepharose 6B-N-acetyl-d-galactosamine and Sepharose 6B-methylalpha-d-mannoside, and also by lectin affinity chromatography using concanavalin A and Lens culinaris lectin as ligands. According to preferential monosaccharide specificity, salt-soluble lectins of corn seed comprise at least two distinct types: N-acetyl-d-galactosamine-interactive and mannose-interactive lectins. The extracted lectins are unstable, with a tendency to form aggregates during storage.
Janković
M
M
Institute of Endocrinology, Immunology and Nutrition-INEP, Banatska 31b, 11080 Zemun, Yugoslavia.
Cuperlović
M
M
Hajduković
L
L