Detection of glycosylated and deglycosylated extensin precursors by indirect competitive ELISA.
A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid quantitation of the glycosylated and deglycosylated forms of the monomeric soluble extensin precursor subunits P1 and P2. A log-linear response range for each kind of precursor in the competition curve was between 0.01 and 100 nanograms per milliliter.
Conrad
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TA
Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824.
Lamport
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DT
Hammerschmidt
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