Evidence for the occurrence of, and possible physiological role for, cyanobacterial calmodulin.
Phosphate uptake by the blue-green alga Oscillatoria limnetica Lemmerman is stimulated by micromolar concentrations of Ca(2+). The calmodulin antagonists 4-(3-[2(trifluoromethyl)phenylthiazin-10-yl]propyl)-1-piperazine ethanol-HCl and its monofluoro-analog inhibit orthophosphate uptake of Oscillatoria limnetica by over 97% implying involvement of calmodulin in this process. A calmodulin-like protein was quantitated in cell-free extracts from O. limnetica by radioimmunoassay.
Kerson
G W
GW
Department of Biology, Queen's University, Kingston, Ontario K7L 3N6 Canada.
Miernyk
J A
JA
Budd
K
K