Lung regeneration: implantation of fetal rat lung fragments into adult rat lung parenchyma.
OBJECTIVE
The capability of regeneration of lung tissues in adults is limited after chronic destruction. Bone marrow-derived stem cells, retinoic acid, growth factors, and other approaches have been attempted to promote or facilitate this process. We hypothesized that fetal lung tissues, with great potential for growth and differentiation, could be used for lung regeneration.


METHODS
Day 17 fetal lung tissue fragments at the pseudoglandular stage of lung development from Lewis rats were implanted into adult Lewis rat lungs. For group 1, fetal lung fragments were injected into the adult left lung parenchyma; for group 2, fetal fragments were injected with the left lung partially resected; for group 3, adult fragments were injected; and for groups 4 and 5, fetal fragments were implanted into the omentum and subcutaneous tissue, respectively.


RESULTS
The grafts implanted into pulmonary parenchyma were differentiated with opening of the alveolar space after 4 weeks and were advanced further with morphologic features similar to those of neonatal lungs after 8 and 12 weeks. The implants were connected with pulmonary circulation determined by means of perfusion with India ink. These changes appeared to be further enhanced in animals with partial lung resection that might have facilitated the maturation of implanted fetal lung tissues through mechanical factors, soluble factors, or both. Fetal lung tissues did not mature when implanted into the omentum or subcutaneous tissue. Adult lung fragments did not expand after being reimplanted back into the same animal.


CONCLUSIONS
Fetal lung tissue might be an option for further investigation into lung regeneration.
Kenzaki
Koichiro
K
Department of Oncological and Regenerative Surgery, University of Tokushima Graduate School, Tokushima, Japan.
Sakiyama
Shoji
S
Kondo
Kazuya
K
Yoshida
Mitsuteru
M
Kawakami
Yukikiyo
Y
Takehisa
Masatsugu
M
Takizawa
Hiromitsu
H
Miyoshi
Takanori
T
Bando
Yoshimi
Y
Tangoku
Akira
A
Liu
Mingyao
M