title: Viral particles of the endogenous retrovirus ZAM from Drosophila melanogaster use a pre-existing endosome/exosome pathway for transfer to the oocyte.
abstract: BACKGROUND
  Retroviruses have evolved various mechanisms to optimize their transfer to new target cells via late endosomes. Here, we analyzed the transfer of ZAM, a retroelement from Drosophila melanogaster, from ovarian follicle cells to the oocyte at stage 9-10 of oogenesis, when an active yolk transfer is occurring between these two cell types.
  
  
  RESULTS
  Combining genetic and microscopic approaches, we show that a functional secretory apparatus is required to tether ZAM to endosomal vesicles and to direct its transport to the apical side of follicle cells. There, ZAM egress requires an intact follicular epithelium communicating with the oocyte. When gap junctions are inhibited or yolk receptors mutated, ZAM particles fail to sort out the follicle cells.
  
  
  CONCLUSIONS
  Overall, our results indicate that retrotransposons do not exclusively perform intracellular replication cycles but may usurp exosomal/endosomal traffic to be routed from one cell to another.
authors: INSERM, U384, Faculté de Médecine, BP38, 63001 Clermont-Ferrand, France. Emilie.BRASSET@inserm.u-clermont1.fr
authors: E
authors: E
authors: Brasset
authors: A R
authors: AR
authors: Taddei
authors: F
authors: F
authors: Arnaud
authors: B
authors: B
authors: Faye
authors: A M
authors: AM
authors: Fausto
authors: M
authors: M
authors: Mazzini
authors: F
authors: F
authors: Giorgi
authors: C
authors: C
authors: Vaury