Sorbitol-6-phosphate dehydrogenase from loquat fruit.
Sorbitol-6-phosphate dehydrogenase was found in flesh tissue of mature fruit of the loquat (Eriobotrya japonica Lindl. var. Tanaka). The enzyme was purified about 30-fold from the crude extract of the fruit, and was demonstrated to catalyze sorbitol-6-phosphate + NADP right harpoon over left harpoon glucose-6-phosphate + NADPH. The optimal pH values for sorbitol 6-phosphate oxidation and glucose 6-phosphate reduction were 9.8 and 9.1, respectively.
Hirai
M
M
Okitsu Branch, Fruit Tree Research Station, Ministry of Agriculture, Forestry and Fisheries, Okitsu, Shizuoka, 424-02, Japan.