Comparison of ultrasound and electromagnetic field effects on osteoblast growth.
This study compares the mechanisms of ultrasound (US) on osteoblast proliferation with those of pulsed electromagnetic field (PEMF), by different signal transduction pathway inhibitors. The cells were stimulated for 15 min under US or for 2 h under PEMF exposure. Twenty-four h after the beginning of stimulation, the cells were harvested and used for mitochondrial activity test (MTT) analysis. The results showed that there are different transduction pathways for US and PEMF stimulation that lead to an upgrade of osteoblast proliferation, although their pathways all lead to an increase in cytocolic Ca2+ and activation of calmodulin. These findings offer a biochemical mechanism to support the process of ultrasound and PEMF-induced enhanced healing of bone fractures.
Li
Jimmy Kuan-Jung
JK
Center for Nano Bioengineering, Chung Yuan Christian University, Chung Li, Taiwan, Republic of China.
Lin
James Cheng-An
JC
Liu
Hwa-Chang
HC
Sun
Jui-Sheng
JS
Ruaan
Rouh-Chyu
RC
Shih
Chung
C
Chang
Walter Hong-Shong
WH