title: [Effect of nonylphenol on testosterone secretion of rat leydig cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of nonylphenol (NP) on testosterone secretion of Leydig cells.
  
  
  METHODS
  A primary culture system of Leydig cells was set up, followed by identification of Leydig cells with 3beta-HSD staining. After treatment with different concentrations of NP, testosterone secretion was detected and morphological examination was performed.
  
  
  RESULTS
  Following treatment with NP, morphologic changes of Leydig cells were detected, with decreased cell density at high doses of NP. Testosterone increased at lower concentrations of NP while decreased at high concentrations of NP.
  
  
  CONCLUSIONS
  Lower doses of NP can stimulate the secretion of testosterone, but increased exposure to NP will inhibit testosterone secretion of Leydig cells. Besides, high concentrations of NP can cause death of Leydig cells in vitro.
authors: Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Jiangsu, China.
authors: Feng
authors: F
authors: Ying
authors: Yi
authors: Y
authors: Gong
authors: Xiao-dong
authors: XD
authors: Han