title: Regulation by the cAMP cascade of oxygen free radical balance in mammalian cells.
abstract: A study is presented of the effect of the cAMP cascade on oxygen metabolism in mammalian cell cultures. Serum-starvation of the cell cultures resulted in depression of the forward NADH-ubiquinone oxidoreductase activity of complex I, decreased content of glutathione, and enhancement of the cellular level of H2O2. Depressed transcription of cytosolic Cu/Zn-SOD 1, mitochondrial glutathione peroxidase and catalase was also observed. Activation of the cAMP cascade reversed the depression of the activity of complex I and the accumulation of H2O2. The effect of cAMP involved the cAMP-dependent protein kinase.
authors: Department of Medical Biochemistry, Biology and Physics, University of Bari, Bari, Italy.
authors: F
authors: F
authors: Bellomo
authors: C
authors: C
authors: Piccoli
authors: T
authors: T
authors: Cocco
authors: S
authors: S
authors: Scacco
authors: F
authors: F
authors: Papa
authors: A
authors: A
authors: Gaballo
authors: D
authors: D
authors: Boffoli
authors: A
authors: A
authors: Signorile
authors: A
authors: A
authors: D'Aprile
authors: R
authors: R
authors: Scrima
authors: A M
authors: AM
authors: Sardanelli
authors: N
authors: N
authors: Capitanio
authors: S
authors: S
authors: Papa