title: Fluoride-Induced Inhibition of Starch Biosynthesis in Developing Potato, Solanum tuberosum L., Tubers Is Associated with Pyrophosphate Accumulation.
abstract: Pretreatment of discs excised from developing tubers of potato (Solanum tuberosum L.) with 10 millimolar sodium fluoride induced a transient increase in 3-phosphoglycerate content. This was followed by increases in triose-phosphate, fructose 1,6-bisphosphate and hexose-phosphate (glucose 6-phosphate + fructose 6-phosphate + glucose 1-phosphate). The effect of fluoride is attributed to an inhibition of glycolysis and a stimulation of triose-phosphate recycling (the latter confirmed by the pattern of (13)C-labeling [NMR] in sucrose when tissue was supplied with [2-(13)C]glucose). Fluoride inhibited the incorporation of [U-(14)C] glucose, [U-(14)C]sucrose, [U-(14)C]glucose 1-phosphate, and [U-(14)C] glycerol into starch. The incorporation of [U-(14)C]ADPglucose was unaffected. Inhibition of starch biosynthesis was accompanied by an almost proportional increase in the incorporation of (14)C into sucrose. The inhibition of starch synthesis was accompanied by a 10-fold increase in tissue pyrophosphate (PPi) content. Although the subcellular localization of PPi was not determined, a hypothesis is presented that argues that the PPi accumulates in the amyloplast due to inhibition of alkaline inorganic pyrophosphatase by fluoride ions.
authors: Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee DD25DA, United Kingdom.
authors: R
authors: R
authors: Viola
authors: H V
authors: HV
authors: Davies