Immunodetection of the Ferredoxin-NADP Oxidoreductase-Binding Protein Complex in Thylakoids of Different Higher Plant Species.
Monospecific polyclonal antibodies against thylakoid ferredoxin-NADP(+) oxidoreductase and its binding protein from Spinacia oleracea were used to detect the presence of these proteins in different higher plants, including C(3), C(4), and Crassulacean acid metabolism species. A remarkable conservation of antigenic determinants in all the species analyzed was demonstrated for both the reductase and its binding protein. The association of these polypeptides in a complex was detected by immunoprecipitation.
Soncini
F C
FC
Centro de Estudios Fotosintéticos y Bioquímicos (CONICET, F.M.Lillo, U.N.R.), Suipacha 531, 2000 Rosario, Argentina.
Vallejos
R H
RH