title: Mass defect labeling of cysteine for improving peptide assignment in shotgun proteomic analyses.
abstract: A method for improving the identification of peptides in a shotgun proteome analysis using accurate mass measurement has been developed. The improvement is based upon the derivatization of cysteine residues with a novel reagent, 2,4-dibromo-(2'-iodo)acetanilide. The derivitization changes the mass defect of cysteine-containing proteolytic peptides in a manner that increases their identification specificity. Peptide masses were measured using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron mass spectrometry. Reactions with protein standards show that the derivatization of cysteine is rapid and quantitative, and the data suggest that the derivatized peptides are more easily ionized or detected than unlabeled cysteine-containing peptides. The reagent was tested on a 15N-metabolically labeled proteome from M. maripaludis. Proteins were identified by their accurate mass values and from their nitrogen stoichiometry. A total of 47% of the labeled peptides are identified versus 27% for the unlabeled peptides. This procedure permits the identification of proteins from the M. maripaludis proteome that are not usually observed by the standard protocol and shows that better protein coverage is obtained with this methodology.
authors: Department of Chemistry, University of Georgia, Athens, GA 30602, USA.
authors: Hilda
authors: H
authors: Hernandez
authors: Sarah
authors: S
authors: Niehauser
authors: Stacey A
authors: SA
authors: Boltz
authors: Vijay
authors: V
authors: Gawandi
authors: Robert S
authors: RS
authors: Phillips
authors: I Jonathan
authors: IJ
authors: Amster