title: Occurrence of mycotoxins (ochratoxin A, deoxynivalenol) and toxigenic fungi in Moroccan wheat grains: impact of ecological factors on the growth and ochratoxin A production.
abstract: The aim of the present work was to evaluate the contamination of some samples, taken from Moroccan wheat grains, by ochratoxin A (OTA), deoxynivalenol (DON) and the associated toxigenic fungi. Moreover, we focused on the influence of environmental factors on both the growth and OTA production by three strains of Aspergillus. The results showed that only few samples were contaminated by the two mycotoxins (2 samples for OTA and 7 for DON). The main isolated fungi belong to the Aspergillus, Penicillium and Fusarium genus; 74 Aspergillus and 28 Penicillium isolates were tested for their ability to produce OTA. Only 2 A. alliaceus and 14 A. niger were able to synthesize OTA. However, none of Penicillium isolates can produce this toxin under the conditions mentioned. In respect of the effects of the temperature and water activity (aw), the optimal conditions for the growth and OTA production were different. While the optimal conditions of growth for A. alliaceus and A. terreus are 30 degrees C and 0.98 aw, A. niger preferred 0.93-0.95 aw at 25 degrees C, whereas the optimal production of OTA was observed at 30 degrees C for both A. alliaceus and A. niger at 0.93 and 0.99 aw, respectively.
authors: Laboratoire d'Agro-alimentaire et Sécurité Sanitaire des Aliments, UFR de Biochimie Appliquée et Sciences Alimentaires, Faculté des Sciences Dhar El Mahraz, Atlas Fès, Morocco. abhajjaji@yahoo.fr
authors: Abdelouahed
authors: A
authors: Hajjaji
authors: Mostafa
authors: M
authors: El Otmani
authors: Driss
authors: D
authors: Bouya
authors: Amina
authors: A
authors: Bouseta
authors: Florence
authors: F
authors: Mathieu
authors: Sonia
authors: S
authors: Collin
authors: Ahmed
authors: A
authors: Lebrihi