title: [Generation of transgenic mouse of dentin sialoprotein and transgene expression analysis].
abstract: OBJECTIVE
  To generate the transgenic mouse model of DSP and perform transgene expression analysis by RT-PCR.
  
  
  METHODS
  Plasmid pcDNA3.1-CX was constructed by substituting promoter cbeta-actin for CMV promoter of pcDNA3.1, and the ultimate transgenic vector, pcDNA3.1-CX-dsp, was constructed by cloning DSP coding sequence into pcDNA3.1-CX. The pcDNA3.1-CX-dsp plasmid was linearized and microinjected into the male pronucleus of the zygotes. The tail DNA of pups was tested by PCR and Southern blot. A member of F1 generation of one positive mouse was used to perform transgene expression analysis by RT-PCR.
  
  
  RESULTS
  717 embryos were implanted to 29 recipient pseudopregnant mice, 4 of the 67 pups carrying the transgene. Expression of DSP was detected in a member of F1 generation of one positive mouse by RT-PCR.
  
  
  CONCLUSIONS
  Founders of the DSP transgenic mouse were obtained successfully, and the expression of DSP was primarily confirmed.
authors: Department of Oral Medicine, College of Stomatology, Fourth Military Medical University, Xi'an 710032, Shanxi Province, China. hantang@fmmu.edu.cn
authors: Han-tang
authors: HT
authors: Sun
authors: Ming-zhen
authors: MZ
authors: Xiao
authors: Bu-ling
authors: BL
authors: Wu
authors: Qing-ling
authors: QL
authors: Zhu
authors: Jian
authors: J
authors: Fei