title: Interactions of systemic immune response and local wound healing in different burn depths: an experimental study on rats.
abstract: This study aimed to clarify the local and systemic immune responses at different burn depths. Thirty female Sprague-Dawley rats were divided into three groups: full-thickness (F), partial-thickness (P), and Sham (S). Burns were induced on three separate areas on the dorsums of rats. Serum levels of interferon (IFN)-gamma; tumor necrosis factor-alpha; interleukin (IL)-1, IL-6, and IL-10 were measured once in controls and 1 hour after burn, 48 hours after burn, and 7 days after burn in F and P groups. Neutrophils, CD68-positive macrophages, HLA-DR-positive cells, and CD3-positive lymphocytes were graded semiquantitatively, and the wounds were examined once in shams and at 1 hour after burn, 48 hours after burn, and 7 days after burn in F and P groups. IL-6 levels were highest in F group, followed by P group 1 hour after burn. IFN-gamma levels were higher in the F group; IL-1 levels were higher in F and P groups at 1 hour after burn. Local accumulation of macrophages was similar in F and P groups. Lymphocytes were denser in P group at 1 hour after burn, and neutrophils were denser in F group at 7 days after burn. We suggest that early elevations of IL-6 and IFN-gamma prolong inflammation in full-thickness burns. Modulation of proinflammatory cytokines may improve burn wound treatment.
authors: Department of General Surgery, Başkent University, Faculty of Medicine, Ankara, Turkey.
authors: A Ebru
authors: AE
authors: Sakallioglu
authors: Ozgur
authors: O
authors: Basaran
authors: Hamdi
authors: H
authors: Karakayali
authors: B Handan
authors: BH
authors: Ozdemir
authors: Muammer
authors: M
authors: Yucel
authors: Zubeyde
authors: Z
authors: Arat
authors: Mehmet
authors: M
authors: Haberal