A comparison of gene expression in mouse liver and kidney in obstructive cholestasis utilizing high-density oligonucleotide microarray technology.
OBJECTIVE
To assess the effects of obstructive cholestasis on a wider range of gene expression using microarray technology.


METHODS
Male C57BL/6J mice underwent common bile duct ligation (BDL) and were matched with pair-fed sham-operated controls. After 7 d, the animals were sacrificed and total RNA was isolated from livers and kidneys. Equal amounts of RNA from each tissue were pooled for each group and hybridized to Affymetrix GeneChip MG-U74Av2 containing a total of 12488 probe sets. Data analysis was performed using GeneSpring 6.0 software. Northern analysis and immunofluorescence were used for validation.


RESULTS
In sham-operated and BDL mice, 44 and 50% of 12488 genes were expressed in livers, whereas 49 and 51% were expressed in kidneys, respectively. Seven days after BDL, 265 liver and 112 kidney genes with GeneOntology annotation were up-regulated and 113 liver and 36 kidney genes were down-regulated in comparison with sham-operated controls. Many genes were commonly regulated in both tissues and metabolism-related genes represented the largest functional group.


CONCLUSIONS
Following BDL, microarray analysis reveals a broad range of gene alterations in both liver and kidney.
Denk
Gerald U
GU
Liver Center, Yale University School of Medicine, New Haven, Connecticut 06520-8019, USA.
Cai
Shi-Ying
SY
Chen
Wen-Sheng
WS
Lin
Aiping
A
Soroka
Carol J
CJ
Boyer
James L
JL