Root hair cell enhancement in tissue cultures from soybean roots: a useful model system: in vitro Rhizobium symbiosis.
A technique for obtaining large numbers of root hair cells in cell cultures from soybeans is described. The cells were grown on agar containing the Prairie Regional Laboratory B5 (PRL-B5) medium for periods longer than 60 days. Mixed populations of cultured root hair cells and cortical cells were used to study the in vitro association between soybean cells and Rhizobium japonicum. The advantages of these types of root cell cultures in studies of symbiosis are discussed.
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Charles F. Kettering Research Laboratory, 150 East South College Street, Yellow Springs, Ohio 45387.
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