Real-time detection of caspase-2 activation in a single living HeLa cell during cisplatin-induced apoptosis.
Caspase-2 is important for the mitochondrial apoptotic pathway, however, the mechanism by which caspase-2 executes apoptosis remains obscure. We carry out the first measurements of the dynamics of caspase-2 activation in a single living cell by a FRET (fluorescence resonance energy transfer) probe. Two FRET probes are constructed that each encoded a CRS (caspase-2 or caspase-3 recognition site) fused with a cyan fluorescent protein (CFP) and a red fluorescent protein (DsRed) (CFP-CRS-DsRed). Using these probes, we found that during cisplatin-induced apoptosis, caspase-2 activation occurred more slowly than did activation of caspase-3; additionally, caspase-2 activation was initiated much earlier than that of caspase-3.
Lin
Juqiang
J
Huazhong University of Science and Technology, Key Laboratory of Biomedical Photonics of Ministry of Education-Hubei, Bioinformatics and Molecular Imaging Key Laboratory, Wuhan 430074 China.
Zhang
Zhihong
Z
Yang
Jie
J
Zeng
Shaoqun
S
Liu
Bi-Feng
BF
Luo
Qingming
Q