title: Characterization of melanocyte-specific inducible Cre recombinase transgenic mice.
abstract: Conditional Cre-mediated recombination has emerged as a robust method of introducing somatic genetic alterations in an organ-specific manner in the mouse. Here, we generated and characterized mice harboring a 4-hydroxytamoxifen (OHT)-inducible Cre recombinase-estrogen receptor fusion transgene under the control of the melanocyte-specific tyrosinase promoter, designated Tyr::CreER(T2). Cre-mediated recombination was induced in melanocytes in a spatially and temporally controlled manner upon administration of OHT and was documented in embryonic melanoblasts, follicular bulb melanocytes, dermal dendritic melanocytes, epidermal melanocytes of tail skin, and in putative melanocyte stem cells located within the follicular bulge. Functional evidence suggestive of recombination in follicular melanocyte stem cells included the presence of Cre-mediated recombination in follicular bulb melanocytes 1 year after topical OHT administration, by which time several hair cycles have elapsed and the melanocytes residing in this location have undergone multiple rounds of apoptosis and replenishment. These Tyr:: CreER(T2) transgenic mice represent a useful resource for the evaluation of melanocyte developmental genetics, the characterization of melanocyte stem cell function and dynamics, and the construction of refined mouse models of malignant melanoma.
authors: Department of Pathology, University of Vermont, Burlington, Vermont 05405, USA. Marcus.Bosenberg@uvm.edu
authors: Marcus
authors: M
authors: Bosenberg
authors: Viswanathan
authors: V
authors: Muthusamy
authors: David P
authors: DP
authors: Curley
authors: Zhenxiong
authors: Z
authors: Wang
authors: Cara
authors: C
authors: Hobbs
authors: Betsy
authors: B
authors: Nelson
authors: Cristina
authors: C
authors: Nogueira
authors: James W
authors: JW
authors: Horner
authors: Ronald
authors: R
authors: Depinho
authors: Lynda
authors: L
authors: Chin