title: Ars insulator identified in sea urchin possesses an activity to ensure the transgene expression in mouse cells.
abstract: Sea urchin arylsulfatase (Ars) gene locus has features of an insulator, i.e., blocking of enhancer and promoter interaction, and protection of a transgene against positional effects [Akasaka et al. (1999) Cell. Mol. Biol. 45, 555-565]. To examine the effect of Ars insulator on long-term expression of a transgene, the insulator was inserted into LTR of retrovirus vector harboring hrGFP gene as a reporter, and then introduced into mouse myoblast cells. The isolated clones transduced with the reporter gene with or without Ars insulator were cultured for more than 20 wk in the absence of a selection reagent, and the expression of hrGFP was periodically determined. Expression of hrGFP in four clones transduced with the reporter gene without Ars insulator was completely silenced after 20 wk of culture. On the other hand, hrGFP was expressed in all clones with Ars insulator inserted in one of the two different orientations. Histone H3 deacetylation and DNA methylation of the 5'LTR promoter region, signs for heterochromatin and silencing, were suppressed in the clones that were expressing hrGFP. Ars insulator is effective in maintaining a transgene in mouse cells in an orientation-dependent manner, and will be a useful tool to ensure stable expression of a transgene.
authors: Laboratory of Epigenetics, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871. tajima@protein.osaka-u.ac.jp
authors: Shoji
authors: S
authors: Tajima
authors: Keiko
authors: K
authors: Shinohara
authors: Maiko
authors: M
authors: Fukumoto
authors: Reiko
authors: R
authors: Zaitsu
authors: Junichi
authors: J
authors: Miyagawa
authors: Shinjiro
authors: S
authors: Hino
authors: Jun
authors: J
authors: Fan
authors: Koji
authors: K
authors: Akasaka
authors: Masao
authors: M
authors: Matsuoka