title: Transport kinetics and metabolism of exogenously applied putrescine in roots of intact maize seedlings.
abstract: Putrescine metabolism, uptake, and compartmentation were studied in roots of hydroponically grown intact maize (Zea mays L.) seedlings. In vivo analysis of exogenously applied putrescine indicated that the diamine is primarily metabolized by a cell wall-localized diamine oxidase. Time-dependent kinetics for putrescine uptake could be resolved into a rapid phase of uptake and binding within the root apoplasm, followed by transport across the plasma membrane that was linear for 30 to 40 minutes. Concentration-dependent kinetics for putrescine uptake (between 0.05 and 1.0 millimolar putrescine) appeared to be nonsaturating but could be resolved into a saturable (V(max) 0.397 micromoles per gram fresh weight per hour; K(m) 120 micromolar) and a linear component. The linear component was determined to be cell wall-bound putrescine that was not removed during the desorption period following uptake of [(3)H]putrescine. These results suggest that a portion of the exogenously applied putrescine can be metabolized in maize root cell walls by diamine oxidase activity, but the bulk of the putrescine is transported across the plasmalemma by a carrier-mediated process, similar to that proposed for animal systems.
authors: Department of Soil, Crop, and Atmospheric Sciences, United States Department of Agriculture-Agricultural Research Service, Cornell University, Ithaca, New York 14853.
authors: J M
authors: JM
authors: Ditomaso
authors: J J
authors: JJ
authors: Hart
authors: L V
authors: LV
authors: Kochian