title: Hygromycin resistance gene cassettes for vector construction and selection of transformed rice protoplasts.
abstract: Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Pstl, and Sacll sites in the coding sequence of a hygromycin B phosphotransferase gene (hph) from Escherichia coli were eliminated. The mutated hph genes were used to form gene cassettes flanked by EcoRI-Sacll-Kpnl-Hindlll sites. Hygromycin resistance of wild-type and mutated hph genes was indistinguishable in E. coli and rice protoplast growth assay.
authors: Department of Plant Pathology and Crop Physiology, College of Agriculture, Louisiana State University, Baton Rouge, Louisiana 70803-1720.
authors: Z
authors: Z
authors: Zheng
authors: A
authors: A
authors: Hayashimoto
authors: Z
authors: Z
authors: Li
authors: N
authors: N
authors: Murai