title: [Protective effects of hydroxyethylpuerarin against brain astrocytes injury induced by hydrogen peroxide].
abstract: OBJECTIVE
  To study the protective effects of hydroxyethylpuerarin against the injury of astrocytes induced by hydrogen peroxide (H2O2).
  
  
  METHODS
  Experiments were performed with cells from passage 4. Plasma membrane integrity was measured by lactate dehydrogenase (LDH) release. The occurrence of apoptosis was measured by flow cytometry. The glutamate uptake of astrocytes was studied with [3H]-glutamate incorporation. Intracellular superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were assessed by automatic biochemistry analyzer.
  
  
  RESULTS
  Compared with H2O2 injured group, the occurrence of apoptosis, levels of LDH release and intracellular MDA of astrocytes reduced in hydroxyethylpuerarin pre-treated groups, but the glutamate uptake and intracellular SOD activity of astrocytes increased.
  
  
  CONCLUSIONS
  Hydroxyethylpuerarin could reduce the occurrence of apoptosis and improve neurotrophic function of astrocytes, which may be related with its antioxidant effects during oxidative stress.
authors: Institute of Pharmacology, School of Medicine, Shandong University, Jinan 250012, China.
authors: Bin
authors: B
authors: Zhang
authors: Xin-bing
authors: XB
authors: Wei
authors: Hui-qing
authors: HQ
authors: Liu
authors: Li-xiang
authors: LX
authors: Wang
authors: Ru
authors: R
authors: Sun
authors: Xiu-mei
authors: XM
authors: Zhang