Partial Purification of the NADH-Nitrate Reductase Complex from Chlorella pyrenoidosa.
The nitrate reductase complex from Chlorella pyrenoidosa has been purified by a procedure which includes as main steps, ammonium sulfate fractionation, polyethylene glycol treatment, and DEAE-cellulose chromatography. The Michaelis constants for NADH, FAD, and NO(3) (-) in the NADH-nitrate reductase assay are 10 mum, 2.6 mum, and 0.23 mm, respectively. Heat treatment exerts varying effects on the enzymatic activities associated with the nitrate reductase complex.
Schloemer
R H
RH
Department of Biology, University of Virginia, Charlottesville, Virginia 22903.
Garrett
R H
RH