title: Metabolism of Indole-3-Acetic Acid: II. Oxindole Pathway in Parthenocissus tricuspidata Crown-Gall Tissue Cultures.
abstract: An indoleacetic acid oxidase preparation from an acetone powder of Parthenocissus tricuspidata crown-gall tissue has been examined. An intermediate in the reaction is 3-hydroxymethyloxindole and nonenzymic conversion of it to 3-methyleneoxindole was observed. Neither reaction mixtures nor 3-methyleneoxindole have any auxin-like activity in Avena or wheat coleoptile bioassays. In vivo studies show that although 53% decarboxylation of indoleacetic acid was observed in 48 hours, only a small amount of 3-methyloxindole could be recovered from the medium. The other decarboxylated products remain to be identified but are not 3-hydroxymethyloxindole or 3-methyleneoxindole.
authors: Departments of Biology, Entomology and Pesticide Research Laboratory, The Pennsylvania State University, University Park, Pennsylvania 16802.
authors: R H
authors: RH
authors: Hamilton
authors: H E
authors: HE
authors: Meyer
authors: R E
authors: RE
authors: Burke
authors: C S
authors: CS
authors: Feung
authors: R O
authors: RO
authors: Mumma