title: Column-switching high-performance liquid chromatographic analysis of fluvastatin in rat plasma by direct injection.
abstract: A column-switching high-performance liquid chromatographic (HPLC) method has been developed and validated for quantification of fluvastatin in rat plasma. Plasma samples were diluted with an equal volume of mobile phase, i.e. acetonitrile-5 mM potassium phosphate buffer (pH 6.8) (15:85, v/v), and the mixture was directly injected onto the HPLC system. The analyte was enriched in a pre-treatment column, while endogenous components were eluted to waste. The analyte was then back-flushed onto an analytical column and quantified with fluorescence detection (lambdaex=305 nm; lambdaem=390 nm). The standard curve for the drug was linear in the range 0.5-100 ng mL(-1) in rat plasma. The limit of quantitation for plasma was found to be 0.5 ng mL(-1). This method has been fully validated and shown to be specific, accurate and precise. The method is simple and rapid because of a minimized sample preparation and appears to be useful for the pharmacokinetic study of fluvastatin.
authors: Division of Biopharmaceutics, Department of Pharmacology, National Institute of Toxicological Research, Korea Food and Drug Administration, Nokbun-dong 5, Eunpyung-Ku, Seoul, Republic of Korea.
authors: So Young
authors: SY
authors: Um
authors: Sung Hee
authors: SH
authors: Jung
authors: Seo Jeong
authors: SJ
authors: Jung
authors: Joo Il
authors: JI
authors: Kim
authors: Soo Youn
authors: SY
authors: Chung
authors: Hwa Jeong
authors: HJ
authors: Lee
authors: Sang Beom
authors: SB
authors: Han
authors: Sun Ok
authors: SO
authors: Choi