Spatial Distribution of Flavonoid Conjugates in Relation to Glucosyltransferase and Sulfotransferase Activities in Flaveria bidentis.
The spatial distribution of sulfated and glucosylated flavonols as well as of the enzymes involved in the later steps of their biosynthesis, sulfotransferase and glucosyltransferase, were investigated in the shoots of Flaveria bidentis. The highest amounts of both types of flavonoid conjugates (as micromole per gram fresh weight) and the highest activities of their enzymes (as picokat per milligram) were detected in the terminal bud and the first pair of leaves. Sulfotransferase activity was also highest in the upper stem segments and in the basal section of the leaves. Western blot analysis of protein extracts showed that variations in sulfotransferase activity in different tissues correlate well with the amounts of immunodetected enzyme protein. These results were discussed in relation to the possible role of conjugated flavonoids in plant growth.
Hannoufa
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Plant Biochemistry Laboratory, Department of Biology, Concordia University, Montréal, Québec, Canada H3G 1M8.
Varin
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Ibrahim
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