title: [Construction and identification for cell strain of anti-human sperm protein 22 monoclonal antibodies].
abstract: OBJECTIVE
  To prepare monoclonal antibodies of mice anti-human sperm protein 22 (SP22) and to identify their specificities.
  
  
  METHODS
  BALB/c mice were immunized with human SP22, monoclonal antibodies were prepared by hybridoma technique and the sensitivity and specificity of SP22 McAb were investigated by ELISA and Western-blot assay, respectively. The distribution of human SP22 in sperm were shown by immunohistochemistry.
  
  
  RESULTS
  Three strains of hybridoma cells were obtained, with the affinity constant (K) of 1.0 x 10(7) L/mol and the titers were 1:10(3) and 1:3,200 in the mixed supernatant of cell cultures and abdominal dropsy, respectively. IgG isotype of the antibody was identified as IgG1. Western blot demonstrated that there was a specific recognition between human SP22 and the obtained monoclonal antibody. Immunohistochemistry displayed that human SP22 mainly distributed on the acrosome surface of human sperm.
  
  
  CONCLUSIONS
  The monoclonal antibodies of anti-human 22 was prepared by the technique of hybridoma cell has higher titer and specificity, which can combine specially with the SP22 protein on the surface of human sperm.
authors: Department of Microbiology and Immunology, NJMU, Nanjing, Jiangsu, China.
authors: Xin-gang
authors: XG
authors: Wang
authors: Chuan-dan
authors: CD
authors: Wan
authors: Yu-feng
authors: YF
authors: Huang