title: Cleavable hydrophilic linker for one-bead-one-compound sequencing of oligomer libraries by tandem mass spectrometry.
abstract: We have developed a method for the rapid and unambiguous identification of sequences of hit compounds from one-bead-one-compound combinatorial libraries of peptide and peptoid ligands. The approach uses a cleavable linker that is hydrophilic to help reduce nonspecific binding to biological samples and allows for the attachment of a halogen tag, which greatly facilitates post-screening sequencing by tandem mass spectrometry (MS/MS). The linker is based on a tartaric acid unit, which, upon cleavage from resin, generates a C-terminal aldehyde. This aldehyde can then be derivatized with a bromine-containing amino-oxy compound that serves as an isotope tag for subsequent MS/MS analysis of y-ion fragments. We have applied this linker and method to the syntheses of a number of peptoids that vary in sequence and length and have also demonstrated single-bead sequencing of a peptoid pentamer. The linker is also shown to have very low levels of nonspecific binding to proteins.
authors: Chiron Corporation, Emeryville, California 94608, USA.
authors: Margot G
authors: MG
authors: Paulick
authors: Kathryn M
authors: KM
authors: Hart
authors: Kristin M
authors: KM
authors: Brinner
authors: Meiliana
authors: M
authors: Tjandra
authors: Deborah H
authors: DH
authors: Charych
authors: Ronald N
authors: RN
authors: Zuckermann