Diverse niches within multiple myeloma bone marrow aspirates affect plasma cell enumeration.
A basic criterion for the diagnosis of multiple myeloma is plasma cell enumeration within the bone marrow (BM). This report showed that flow cytometry under-estimated the number of plasma cells in BM aspirates by an average of 60%, compared with morphological evaluation. The discrepancy was partially because BM smears contain cells associated with the lipid-enriched spicules. In contrast, flow cytometry is performed on the BM fluid, which is depleted of the lipid-adhesive plasma cells. This discrepancy may point to different plasma cell subpopulations associated with diverse niches within the BM.
Nadav
Liat
L
The Molecular Cell Biology Department, Weizmann Institute of Science, Rehovot, Israel.
Katz
Ben-Zion
BZ
Baron
Shoshana
S
Yossipov
Lydia
L
Polliack
Aaron
A
Deutsch
Varda
V
Geiger
Benjamin
B
Naparstek
Ellizabeth
E