Isolation of nucleoli and localization of ribonucleic Acid polymerase I from soybean hypocotyl.
An effective method for the isolation of nucleoli from auxin-treated soybean (Glycine max, var. Wayne) hypocotyl was developed by polytron homogenization and sucrose gradient centrifugation. The nucleoli expressed only the alpha-amanitin-insensitive RNA synthetic activity. This activity chromatographed as RNA polymerase I on DEAE-cellulose. It appears that the plant nucleolus, like the animal nucleolus, is the site of localization for RNA polymerase I.
Lin
C Y
CY
Department of Botany, University of Georgia, Athens, Georgia 30602.
Guilfoyle
T J
TJ
Chen
Y M
YM
Key
J L
JL