title: Structural analysis of water-soluble glucans from the root of Angelica sinensis (Oliv.) Diels.
abstract: Two water-soluble glucans (designated APS-1cI and APS-1cII) were extracted from the roots of Angelica sinensis (Oliv.) Diels and further purified by anion-exchange and gel-filtration chromatography. Their molecular weights were determined to be 1.7 x 10(5) and 3.9 x 10(4)Da, respectively. The structures of the purified glucans were investigated by a combination of chemical and instrumental analysis, such as methylation analysis, periodate oxidation, GC-MS, as well as FTIR and NMR spectroscopy ((1)H, (13)C, H-H COSY, HSQC, HMBC, TOCSY and NOESY). The data obtained indicated that APS-1cI was a linear alpha-glucan composed of only (1-->6)-alpha-D-Glcp, and APS-1cII had a repeating unit consisting of (1-->4)-alpha-D-Glcp and (1-->6)-alpha-D-Glcp in a molar ratio of 4:1. Such glucans isolated from A. sinensis (Oliv.) Diels have not been previously reported.
authors: Department of Pharmacology, School of Pharmacy, the Fourth Military Medical University, Xi'an, China.
authors: Wei
authors: W
authors: Cao
authors: Xiao-Qiang
authors: XQ
authors: Li
authors: Li
authors: L
authors: Liu
authors: Minchang
authors: M
authors: Wang
authors: Hui-Ting
authors: HT
authors: Fan
authors: Chen
authors: C
authors: Li
authors: Zhengguang
authors: Z
authors: Lv
authors: Xiaojing
authors: X
authors: Wang
authors: Qibing
authors: Q
authors: Mei