title: Fluorogenic phospholipid substrate to detect lysophospholipase D/autotaxin activity.
abstract: [reaction: see text] Lysophospholipase D (lysoPLD), also known as autotaxin (ATX), is an important source of the potent mitogen lysophosphatidic acid (LPA). Two fluorogenic substrate analogues for lysoPLD were synthesized in nine steps from (S)-PMB-glycerol. The substrates (FS-2 and FS-3) show significant increases in fluorescence when treated with recombinant ATX and have potential applications in screening for this emerging drug target.
authors: Echelon Biosciences Inc., Salt Lake City, Utah 84108, USA. cferguson@echelon-inc.com
authors: Colin G
authors: CG
authors: Ferguson
authors: Cleve S
authors: CS
authors: Bigman
authors: Robyn D
authors: RD
authors: Richardson
authors: Laurens A
authors: LA
authors: van Meeteren
authors: Wouter H
authors: WH
authors: Moolenaar
authors: Glenn D
authors: GD
authors: Prestwich