Nonaqueous purification of maize mesophyll chloroplasts.
A nonaqueous fractionation method to obtain highly purified mesophyll chloroplasts from lyophilized leaves of Zea mays L. is described. The levels of several metabolites including pyruvate were determined in the purified mesophyll chloroplast fractions which were prepared from leaves exposed to different light intensities. The role of pyruvate in the regulation of pyruvate,Pi dikinase in these chloroplasts under different light intensities is discussed.
Usuda
H
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Laboratory of Chemistry, Faculty of Medicine, Teikyo University, Ohtsuka, Hachioji, Tokyo, Japan, 192-03.