PCR amplification of the IS6110 insertion element of Mycobacterium tuberculosis in fecal samples from patients with intestinal tuberculosis.
PCR amplification of insertion element IS6110 of Mycobacterium tuberculosis in fecal samples was evaluated in the diagnosis of intestinal tuberculosis (ITB). The numbers of samples that tested positive by PCR with SalI digestion were 16/18 untreated-ITB samples, 0/8 treated-ITB samples, 12/14 smear-positive pulmonary tuberculosis samples, and 0/30 control samples. The sensitivity, specificity, positive predictive value, and negative predictive value of fecal PCR were 88.8%, 100%, 100%, and 93.7%, respectively.
Balamurugan
Ramadass
R
Department of Gastrointestinal Sciences, Christian Medical College, Ida Scudder Road, Vellore 632004, Tamil Nadu, India.
Venkataraman
Subramanian
S
John
K R
KR
Ramakrishna
Balakrishnan S
BS