Lysine-ketoglutarate reductase activity in developing maize endosperm.
Lysine-ketoglutarate reductase activity was detected and characterized in the developing endosperm of maize (Zea mays L.). The enzyme showed specificity for its substrates: lysine, alpha-ketoglutarate, and NADPH. Formation of the reaction product saccharopine was demonstrated. The pH optimum of the enzyme was close to 7, and the K(m) for lysine and alpha-ketoglutarate were 5.2 and 1.8 millimolar, respectively.
Arruda
P
P
Department of Genetics and Evolution, Instituto de Biologia, Universidade Estadual de Campinas, 13100 Campinas, SP., Brazil.
Sodek
L
L
da Silva
W J
WJ