title: Altered expression of type XIII collagen in keratoconus and scarred human cornea: Increased expression in scarred cornea is associated with myofibroblast transformation.
abstract: OBJECTIVE
  Type XIII collagen (ColXIII) is a transmembrane protein thought to be involved in cell-cell and cell-matrix interactions. We report here on its presence in the normal human cornea and compare the results for keratoconus and scarred corneas.
  
  
  METHODS
  Immunohistochemistry and in situ hybridization were applied to human corneal samples obtained by penetrating keratoplasty.
  
  
  RESULTS
  In the normal human cornea, ColXIII was immunolocalized to the corneal epithelial cells, and to a lesser degree to the stromal keratocytes. The keratoconus cases showed otherwise similar results, but in areas containing Bowman membrane disruptions showed thinned epithelial cells reduced immunostaining for ColXIII, whereas occasionally pronounced immunoreactivity was seen in the stromal keratocytes. The corneal scar samples contained highly increased ColXIII immunostaining by stromal cells in the fibrotic foci, whereas the peripheral areas showed less intense immunostaining. In situ hybridization confirmed that the corneal epithelium and keratocytes actively synthesize the transcript. Immunostaining with alphaSMA revealed that a substantial proportion of the ColXIII mRNA-expressing cells in the stromal scar tissues was myofibroblasts and that these areas lack CD34 immunoreactivity.
  
  
  CONCLUSIONS
  The results indicate that ColXIII, which is predominantly confined to the basal corneal cells in the normal cornea, may have a role in the adhesion of corneal epithelial cells to each other and to the underlying basement membrane. Additionally, highly increased expression in scarred corneas suggests that it participates in the corneal wound healing process.
authors: Department of Ophthalmology, Helsinki University Central Hospital, Helsinki, Finland. mmaatta@mailcity.com
authors: Marko
authors: M
authors: Määttä
authors: Timo
authors: T
authors: Väisänen
authors: Marja-Riitta
authors: MR
authors: Väisänen
authors: Taina
authors: T
authors: Pihlajaniemi
authors: Timo
authors: T
authors: Tervo