Gibberellic Acid Activates Chromatin-bound DNA-dependent RNA Polymerase in Wounded Potato Tuber Tissue.
Chromatin-bound DNA-dependent RNA polymerases react upon wounding of white potato tuber tissues with an increase in activity, which is additionally enhanced to 300% in the presence of 0.1 micromolar gibberellic acid (GA(3)). 2,4-Dichlorophenoxyacetic acid is only weakly effective and indoleacetic acid not at all. Wounding and treatment with GA(3) affect template availability of chromatin only slightly. The hormone has no effect on chromatin-bound RNA polymerases, if added in vitro.The enzymes from intact, wounded, and hormone-treated tissues possess similar characteristics: their activity is dependent on the presence of all four ribonucleotides and a divalent cation such as Mg(2+) or Mn(2+). However, the sensitivity of the enzymes from different preparations toward alpha-amanitin differs. Total RNA polymerase activity of chromatin was inhibited by alpha-amanitin to about 44% in intact, to about 22% in wounded, and only 15% in GA(3)-treated tissues. The relative activities of polymerases I and II were estimated by varying the (NH(4))(2)SO(4) and alpha-amanitin concentrations in the assay system. It is evident that GA(3) preferentially stimulates polymerase I and hence ribosomal RNA synthesis. RNA polymerase II is but slightly affected by GA(3). Nearest neighbor frequency analysis revealed that the RNA synthesized by the enzymes from the intact tuber is different from that of wounded or GA(3)-treated tissues.
Wielgat
B
B
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.