title: Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus.
abstract: The structures of two major phosphoglycolipids from the thermophilic bacteria Thermus oshimai NTU-063, Thermus thermophilus NTU-077, Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220 were determined using spectroscopic and chemical analyses to be 2'-O-(1,2-diacyl-sn-glycero-3-phospho) -3'-O-(alpha-N-acetyl-glucosaminyl)-N-glyceroyl alkylamine [PGL1 (1)] and the novel structure 2'-O-(2-acylalkyldio-1-O-phospho)-3'-O-(alpha-N-acetylglucosaminyl)-N-glyceroyl alkylamine [PGL2 (2)]. PGL2 (2) is the first phosphoglycolipid identified with a 2-acylalkyldio-1-O-phosphate moiety. The fatty acids of the phosphoglycolipids are mainly iso-C(15:0), -C(16:0), and -C(17:0) and anteiso-C(15:0) and -C(17:0). The ratios of PGL2 (2) to PGL1 (1) are significantly altered when grown at different temperatures for three strains, T. thermophilus NTU-077, M. ruber NTU-124, and M. taiwanensis NTU-220, but not for T. oshimai NTU-063. Accordingly, the ratios of iso- to anteiso-branched fatty acids increase when grown at the higher temperature.
authors: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors: Yu-Liang
authors: YL
authors: Yang
authors: Feng-Ling
authors: FL
authors: Yang
authors: Shu-Chuan
authors: SC
authors: Jao
authors: Mao-Yen
authors: MY
authors: Chen
authors: San-San
authors: SS
authors: Tsay
authors: Wei
authors: W
authors: Zou
authors: Shih-Hsiung
authors: SH
authors: Wu