C1qRP (CD93) expression on peripheral blood monocytes in patients with systemic lupus erythematosus.
Aim of the study was to compare the expression of monocytes C1qRp (CD93) in patients with systemic lupus erythematosus (SLE) with that of healthy controls and to determine the influence of glucocorticoids and LPS on C1qRp expression. Thirty-six SLE patients and 20 healthy controls were analyzed by flow cytometry. Additionally, monocytes from five patients and five controls were cultured and stimulated with dexamethasone, interferon-gamma and LPS, respectively, before the measurement of C1qRp expression. There was no difference in C1qRp expression between SLE and HC. SLE patients with no or only low dose steroids had a significantly higher C1qRp expression than those with higher doses. However, in vitro dexamethasone did not stimulate or down-regulate C1qRp expression. Upon LPS stimulation, C1qRp was significantly up regulated on monocytes both from patients and from controls. In conclusion, C1qRp expression and regulation was not altered in SLE patients. Possible relations with disease activity and medication need further investigations.
Moosig
Frank
F
Second Medical Department, University Hospital of Schleswig Holstein, Campus Kiel, Chemnitzstr. 33, 24116 Kiel, Germany. F.Moosig@med2.uni-kiel.de
Fähndrich
Erika
E
Knorr-Spahr
Anja
A
Böttcher
Sebastian
S
Ritgen
Matthias
M
Zeuner
Rainald
R
Kneba
Michael
M
Schröder
Johann O
JO