Catharanthus roseus shoot cultures for the production of monoterpenoid indole alkaloids.
A protocol for the establishment of in vitro shoot cultures of Catharanthus roseus is described. Shoots can be maintained for more than 1 yr without evidence of tissue vitrification, disaggregation, or callus formation. Vindoline was the main alkaloid accumulated, reaching values similar to those found in leaves from field-grown plants, after a long period of culture. An induction methodology to reduce such waiting time is also presented.
Hernández-Domínguez
Elizabeta
E
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, México.
Campos-Tamayo
Freddy
F
Carrillo-Pech
Mildred
M
Vázquez-Flota
Felipe
F