title: Glycolate Metabolism and Excretion by Chlamydomonas reinhardtii.
abstract: The flux of glycolate through the C(2) pathway in Chlamydomonas reinhardtii was estimated after inhibition of the pathway with aminooxyacetate (AOA) or aminoacetonitrile (AAN) by measurement of the accumulation of glycolate and glycine. Cells grown photoautotrophically in air excreted little glycolate except in the presence of 2 mm AOA when they excreted 5 micromoles glycolate per hour per milligram clorophyll. Cells grown on high CO(2) (1-5%) when transferred to air produced three times as much glycolate, with half of the glycolate metabolized and half excreted. The lower amount of glycolate produced by the air-grown cells reflects the presence of a CO(2) concentrating mechanism which raises the internal CO(2) level and decreases the ribulose-1,5-bisP oxygenase reaction for glycolate production. Despite the presence of the CO(2) concentrating mechanism, there was still a significant amount of glycolate produced and metabolized by air-grown Chlamydomonas. The capacity of these cells to metabolize between 5 and 10 micromoles of glycolate per hour per milligram chlorophyll was confirmed by measuring the biphasic uptake of added labeled glycolate. The initial rapid (<10 seconds) phase represented uptake of glycolate; the slow phase represented the metabolism of glycolate. The rates of glycolate metabolism were in agreement with those determined using the C(2)-cycle inhibitors during CO(2) fixation.
authors: Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824.
authors: J V
authors: JV
authors: Moroney
authors: B J
authors: BJ
authors: Wilson
authors: N E
authors: NE
authors: Tolbert