title: Immunological Characterization of a Tapetal Protein in Developing Anthers of Lilium longiflorum.
abstract: Antiserum was raised in rabbits against a lily (Lilium longiflorum) anther-specific protein (LLA-15). Monospecific anti-LLA-15 antibodies were prepared to investigate the distribution of LLA-15 during anther development in a variety of flowering plants. Immunoblot analyses of total protein from floral and vegetative organs confirmed that LLA-15 or LLA-15-like proteins accumulated to detectable levels only in a discrete stage of anther development. In situ localization using anti-rabbit immunoglobulin G conjugated with gold particles confirmed that LLA-15 was specifically localized in the tapetal tissue of lily anthers. The maximal level of LLA-15 was strictly coincident with the peak of tapetal secretory functions. Immunoblots of two-dimensional polyacrylamide gels of lily anther proteins indicated that the seven LLA-15 isoforms ranged from isoelectric point 5.6 to 6.1. In vitro translation of lily anther mRNAs showed that four of these isoforms were primary products, the additional three being a result of posttranslational processing of the primary translation products.
authors: Department of Botany and Plant Sciences, University of California, Riverside, California 92521.
authors: C S
authors: CS
authors: Wang
authors: L L
authors: LL
authors: Walling
authors: K J
authors: KJ
authors: Eckard
authors: E M
authors: EM
authors: Lord