title: Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes.
abstract: We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-react with 1 to 4 nonvaccine serotypes, could be distinguished using serotype-specific antisera.
authors: Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia.
authors: Fanrong
authors: F
authors: Kong
authors: Mitchell
authors: M
authors: Brown
authors: Archcna
authors: A
authors: Sabananthan
authors: Xianyu
authors: X
authors: Zeng
authors: Gwendolyn L
authors: GL
authors: Gilbert