title: Chromosome 2q24.2 is lost in sporadic but not in BRCA1-associated ovarian carcinomas.
abstract: OBJECTIVE
  Comparison between BRCA1-associated and sporadic ovarian carcinomas is a potential method to identify candidate modifier gene/s involved in the carcinogenic pathway of either or both groups. A previous study identified a significant difference in the frequency of copy number gain at 2q24-q32 by comparing BRCA1-associated and sporadic ovarian tumour specimens using comparative genomic hybridisation (CGH). The present study aimed to investigate the reported allelic imbalance at 2q24-32 by amplification of several microsatellite markers at the region by quantitative microsatellite analysis (QuMA) using Taqman at the same region identified as a site of allelic imbalance.
  
  
  METHODS
  The copy number of the genomic region in 2q24-32 was established in 21 BRCA1-associated ovarian carcinomas and 14 sporadic cases using quantitative microsatellite polymerase chain reaction (PCR). Statistical analysis was performed using permutation test analysis.
  
  
  RESULTS
  A significant loss at D2S156 marker (2q24.2) (p = 0.026) compared with the other three markers at 2q24-32 was found in the sporadic cohort but not in the BRCA1-associated group (p = 0.385).
  
  
  CONCLUSIONS
  Our data do not support the association between copy number gain at 2q24-32 and BRCA1 mutation status in ovarian cancers reported previously. The novel finding of the present study was significant loss at 2q24.2 in sporadic ovarian cancers.
authors: Oncology Research Centre, Prince of Wales Hospital, Randwick, Australia. Morteza.Aghmesheh@sesiahs.health.nsw.gov.au
authors: Morteza
authors: M
authors: Aghmesheh
authors: Zhenhe
authors: Z
authors: Suo
authors: Michael
authors: M
authors: Friedlander
authors: Jahn M
authors: JM
authors: Nesland
authors: Janne
authors: J
authors: Kaern
authors: Michael
authors: M
authors: Stewart
authors: Kconfab
authors: Anne
authors: A
authors: Dorum
authors: Katherine M
authors: KM
authors: Tucker
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authors: MF
authors: Buckley