Purification of myo-Inositol 1-Phosphate Synthase from Rice Cell Culture by Affinity Chromatography.
myo-Inositol 1-phosphate synthase (EC 5.5.1.4) is the enzyme which catalyzes the synthesis of the precursor for the myo-inositol oxidation pathway. Rice callus grown in suspension culture provides a good source of plant enzyme. Use has been made of a noncompetitive inhibitor to prepare an affinity column for this enzyme. With this column, the enzyme from rice callus has been purified 1500-fold in a single step, about 9000-fold over-all, to a specific activity of 0.078 units per milligram of protein. This is an order of magnitude greater than previous purifications of the plant enzyme.
Funkhouser
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Division of Cell and Molecular Biology, State University of New York at Buffalo, Buffalo, New York 14214.
Loewus
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