title: Characterization of a novel human protein phosphatase 2C family member, PP2Ckappa.
abstract: A novel member of human protein phosphatase 2C gene named PP2Ckappa was isolated from a human fetal brain cDNA library. The 2.0 kb cDNA encodes a 372 amino acid polypeptide with an intact protein phosphatase 2C (PP2C) catalytic domain. Reverse transcription-PCR (RT-PCR) revealed that the PP2Ckappa was widely expressed in normal human tissues. Transient transfection suggested that PP2Ckappa was localized in the nucleus in AD293 cells. Recombinant Trx-His-PP2Ckappa showed phosphatase activity toward p-nitrophenyl phosphate (pNPP), as well as oligopeptides containing phospho-threonine residues. Furthermore, the overexpression of PP2Ckappa distinctly activated the heat shock transcription factor pathway in eukaryotic cells.
authors: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, PR China.
authors: Jianfeng
authors: J
authors: Dai
authors: Jiyuan
authors: J
authors: Zhang
authors: Yaqiong
authors: Y
authors: Sun
authors: Qihan
authors: Q
authors: Wu
authors: Liyun
authors: L
authors: Sun
authors: Chaoneng
authors: C
authors: Ji
authors: Shaohua
authors: S
authors: Gu
authors: Congjing
authors: C
authors: Feng
authors: Yi
authors: Y
authors: Xie
authors: Yumin
authors: Y
authors: Mao