Production of cybrids in Brassicaceae species.
This chapter describes a method of cytoplasm transfer within the brassicaceae family through Ca-PEG-mediated protoplast fusion. The method includes a protocol of nonmutagenic albinism induction based on spectinomycin-induced plastid ribosome deficiency (PRD). The proposed application of spectinomycin-mediated albinism allows speeding up creation of albino lines, as well as of hybrid production with substituted cytoplasm. According to described method cybrids between Orychophragmus violaceus and Brassica napus, O. violaceus and Lesquerella fendleri have been produced. Methods of further molecular analysis are also presented. The time-scale and reliability of described methods are indicated.
Vasylenko
Maksym
M
Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine.
Ovcharenko
Olga
O
Gleba
Yuri
Y
Kuchuk
Nikolay
N