title: Comparative metabolism of 2,4-dichlorophenoxyacetic Acid in cotyledon and leaf callus from two varieties of soybean.
abstract: Only quantitative differences were observed in the metabolism of [1-(14)C]2,4-dichlorophenoxyacetic acid (2,4-D) by soybean cotyledon callus as influenced by variety (Acme and Amsoy), source of tissue (cotyledon, leaf and root) and age. All tissues metabolized 2,4-D to water-soluble (glycosides) and ether-soluble (primarily amino acid conjugates) metabolites.In young (3- to 4-week-old) Amsoy cotyledon or leaf callus tissue the free 2,4-D increased proportionately with the external 2,4-D concentration while in older (6- to 8-week-old) tissue the amino acid conjugates increased in this manner. Thus, in the older Amsoy tissue apparent regulation of the internal 2,4-D level (1.5-3.5 nmoles per gram fresh weight) was observed. In 3- or 6-week-old Acme cotyledon callus 2,4-D accumulated with an increase in the external 2,4-D concentration with no evidence for regulation of internal 2,4-D levels.
authors: Pesticide Research Laboratory and Graduate Study Center, The Pennsylvania State University, University Park, Pennsylvania 16802.
authors: G H
authors: GH
authors: Davidonis
authors: R H
authors: RH
authors: Hamilton
authors: R O
authors: RO
authors: Mumma