Protein-imprinted polymer with immobilized assistant recognition polymer chains.
Here we introduce a new method for preparing a protein-imprinted polymer with immobilized assistant recognition polymer chains as an additional element of monomer to create effective recognition sites. In this work the bovine serum albumin was used as template and the template protein was selectively assembled with immobilized assistant recognition polymer chains from their library, numerous limited length polymer chains with randomly distributed recognition sites and immobilizing sites. These assemblies of protein and immobilized assistant recognition polymer chains would be adsorbed by the macro porous adsorbent spheres and immobilized by cross-linking polymerization. After removing the template, binding sites that were complementary to the target protein in size, shape and position of recognition groups were exposed, and their confirmation was preserved by the cross-linked structure. The synthesized imprinted polymer was used to adsorb BSA from protein mixtures, and showed a high selectivity.
Guo
Min-Jie
MJ
Key Laboratory of Functional Polymer Materials, Ministry of Education of China, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, PR China.
Zhao
Zhuo
Z
Fan
Yun-Ge
YG
Wang
Chun-Hong
CH
Shi
Lin-Qi
LQ
Xia
Jian-Jun
JJ
Long
Yi
Y
Mi
Huai-Feng
HF