Application of minimal sequence quality values prevents misidentification of the blaSHV type in single bacterial isolates carrying different SHV extended-spectrum beta-lactamase genes.
Nucleotide sequencing is the standard molecular method for determination of the beta-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of bla(SHV) genes, as illustrated by three strains of three different species that each contained two different bla(SHV) alleles, SHV-2 and SHV-12.
Al Naiemi
Nashwan
N
Department of Medical Microbiology, Academic Medical Center, L1-116, P.O. Box 22660, 1100 DD Amsterdam, The Netherlands.
Schipper
Kim
K
Duim
Birgitta
B
Bart
Aldert
A