title: The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22.
abstract: Host answers to pathogen attacks define the course of pathogenic events and decide about the fate of the host organism. Infection with coxsackievirus B3 (CVB3) can induce severe myocarditis and pancreatitis. The interplay between host factors and virus components is crucial for the fate of the infected host. As we have shown before, expression of the pro-apoptotic host protein Siva is significantly increased after CVB3 infection, and infected cells are removed by programmed cell death. Analysis of Siva expressed in Escherichia coli revealed that this protein binds three zinc ions, suggesting a rather complex three-dimensional structure. By screening a human heart cDNA library we found a new interaction partner of Siva. The peroxisomal membrane protein PMP22 may be involved in the host response against CVB3. Previous investigations showed that Siva interacts with the cytoplasmic C-terminus of CD27, a member of the tumor necrosis factor receptor group, and transmits an apoptotic signal. With the help of directed two-hybrid assays we determined the N-terminal part of Siva as the binding region for CD27.
authors: Department of Cell and Molecular Biology, Leibniz-Institute for Natural Product Research and Infection Biology--Hans-Knöll-Institute, Jena, Germany. mnestler@fli-leibniz.de
authors: Matthias
authors: M
authors: Nestler
authors: Ulrike
authors: U
authors: Martin
authors: Peter
authors: P
authors: Hortschansky
authors: Hans-Peter
authors: HP
authors: Saluz
authors: Andreas
authors: A
authors: Henke
authors: Thomas
authors: T
authors: Munder