title: Activation of Avena coleoptile cell wall glycosidases by hydrogen ions and auxin.
abstract: Several cell wall-bound glycosidases present in Avena sativa coleoptiles were assayed by following the hydrolysis of p-nitrophenyl-glycosides. Particular emphasis was placed on characterizing some parameters affecting the activity of beta-galactosidase. The pH optimum of this enzyme is 4.5 to 5.5; it is sensitive to copper ions and p-chloromercuribenzoate treatment and apparently has an exceptionally low turnover rate. Indoleacetic acid treatment enhanced in vivo beta-galactosidase activity of coleoptile segments by 36% over control after 60 minutes. This enhancement was prevented by abscisic acid and cycloheximide. High buffer strengths and low pH reduced the indoleacetic acid-enhanced increase in enzyme activity. These data lend support to the following proposed model of indoleacetic acid action. Indoleacetic acid enhances the release of hydrogen ions into the cell wall which promote the activities of cell wall glycosidases, some of which may participate in the cell extension process.
authors: Departments of Botany and Physical Science, California State University, San Diego, California 92115.
authors: K D
authors: KD
authors: Johnson
authors: D
authors: D
authors: Daniels
authors: M J
authors: MJ
authors: Dowler
authors: D L
authors: DL
authors: Rayle