A highly sensitive method for quantitative determination of abscisic Acid.
An abscisic acid derivative was formed by reaction with pentafluorobenzyl bromide which allowed highly sensitive detection by gas-liquid chromatography with electron capture detection. In comparison to the methyl ester derivative, the pentafluorobenzyl derivative of abscisic acid was four times more sensitive to electron capture detection and was stable at room temperature in the presence of ultraviolet light. Derivatization was rapid and the molecular weight of the new compound was confirmed by gas-liquid chromatography-mass spectrometry.
Michler
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Department of Horticulture, The Ohio Agricultural Research and Development Center, The Ohio State University, Columbus, Ohio 43210.
Lineberger
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Chism
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