Paullinia pinnata extracts rich in polyphenols promote vascular relaxation via endothelium-dependent mechanisms.
Paullinia pinnata L. (Sapindaceae) is an African tropical plant whose roots and leaves are used in traditional medicine for many purposes, especially for erectile dysfunction, but its action mechanism is unknown. P. pinnata root and leaf methanolic extracts are rich in phenolic compounds. This study shows that both extracts are highly antioxidative and induce a slight transcriptional activity of peroxisome proliferator activated receptor-alpha. They also increased and decreased endothelial nitric oxide synthase and endothelin-1 mRNA levels in bovine aortic endothelial cells, respectively. In this study P. pinnata methanolic extracts in cumulative doses elicited in a dose-dependent manner the relaxation of phenylephrine precontracted isolated rat aortic rings. N-nitro-L-arginine methyl ester significantly attenuated the capacity of both extracts to induce arterial relaxation, indicating that this arterial relaxation was mediated by endothelial nitric oxide release. It could be suggested that the arterial relaxation induced by both extracts could be mainly linked to their capacities to inhibit nitric oxide oxidation through their antioxidant properties.
Zamble
Alexis
A
Laboratoire de Pharmacognosie, Faculté de Pharmacie, Université de Lille 2, Lille, France.
Carpentier
Marie
M
Kandoussi
Abdelmejid
A
Sahpaz
Sevser
S
Petrault
Olivier
O
Ouk
Tawarak
T
Hennuyer
Nathalie
N
Fruchart
Jean-Charles
JC
Staels
Bart
B
Bordet
Régis
R
Duriez
Patrick
P
Bailleul
François
F
Martin-Nizard
Françoise
F