[Protective effects of hydroxyethylpuerarin against brain astrocytes injury induced by hydrogen peroxide].
OBJECTIVE
To study the protective effects of hydroxyethylpuerarin against the injury of astrocytes induced by hydrogen peroxide (H2O2).


METHODS
Experiments were performed with cells from passage 4. Plasma membrane integrity was measured by lactate dehydrogenase (LDH) release. The occurrence of apoptosis was measured by flow cytometry. The glutamate uptake of astrocytes was studied with [3H]-glutamate incorporation. Intracellular superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were assessed by automatic biochemistry analyzer.


RESULTS
Compared with H2O2 injured group, the occurrence of apoptosis, levels of LDH release and intracellular MDA of astrocytes reduced in hydroxyethylpuerarin pre-treated groups, but the glutamate uptake and intracellular SOD activity of astrocytes increased.


CONCLUSIONS
Hydroxyethylpuerarin could reduce the occurrence of apoptosis and improve neurotrophic function of astrocytes, which may be related with its antioxidant effects during oxidative stress.
Zhang
Bin
B
Institute of Pharmacology, School of Medicine, Shandong University, Jinan 250012, China.
Wei
Xin-bing
XB
Liu
Hui-qing
HQ
Wang
Li-xiang
LX
Sun
Ru
R
Zhang
Xiu-mei
XM