Chlorpyrifos exacerbating pemphigus vulgaris: a preliminary report and suggested in vitro immunologic evaluation model.
BACKGROUND
There is accumulating evidence on the role of pesticides in the etiology of pemphigus vulgaris (PV).


OBJECTIVE
To determine whether chlorpyrifos, an organophosphate pesticide, is involved in the immunopathology of PV.


METHODS
Normal human skin biopsy specimens incubated with progressively diluted chlorpyrifos solutions were used as indirect immunofluorescence substrates for sera from two PV patients previously exposed to the agent and from healthy controls. Involvement of T-cell lymphocytes was assessed by release of interferon-g in the presence of chlorpyrifos.


RESULTS
In one PV patient, immunofluorescence was strongly positive for the specimen incubated with the pesticide and weakly positive for the specimen incubated with medium alone. Immunofluorescence was negative in the patient under immunosuppression with prednisolone and in all controls. Both patients tested positive on interferon-g assay; controls tested negative.


CONCLUSIONS
Findings suggest an immunopathogenic role of chlorpyrifos in PV. Interferon-g cytokine assay with the pesticide combined with immunofluorescence tests may provide an in vitro diagnostic tool in suspected pesticide-induced/exacerbated pemphigus.
Wohl
Yonit
Y
Department of Dermatology, Tel Aviv-Sourasky Medical Center, and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Goldberg
Ilan
I
Shirazi
Idit
I
Brenner
Sarah
S