Comparison of clonogenic assay with premature chromosome condensation assay in prediction of human cell radiosensitivity.
OBJECTIVE
To determine whether the number of non-rejoining G2-chromatid breaks can predict the radiosensitivity of human cell lines.


METHODS
Cell lines of human ovary carcinoma cells (HO8910), human hepatoma cells (HepG2) and liver cells (L02) were irradiated with a range of doses and assessed both of cell survival and non-rejoining G2-chromatid breaks at 24 h after irradiation. Cell survival was documented by a colony assay. Non-rejoining G2-chromatid breaks were measured by counting the number of non-rejoining G2 chromatid breaks at 24 h after irradiation, detected by the prematurely chromosome condensed (PCC) technique.


RESULTS
A linear-quadratic survival curve was observed in three cell lines, and HepG2 was the most sensitive to gamma-radiation. A dose-dependent linear increase was observed in radiation-induced non-rejoining G2-PCC breaks measured at 24 h after irradiation in all cell lines, and HepG2 was the most susceptible to induction of non-rejoining G2-PCC breaks. A close correlation was found between the clonogenic radiosensitivity and the radiation-induced non-rejoining G2-PCC breaks (r = 0.923). Furthermore, survival-aberration correlations for two or more than two doses lever were also significant.


CONCLUSIONS
The number of non-rejoining G2 PCC breaks holds considerable promise for predicting the radiosensitivity of normal and tumor cells when two or more than two doses lever is tested.
Wang
Zhuan-Zi
ZZ
Graduate School of Chinese Academy of Sciences, Beijing, China. yancz@lzb.ac.cn
Li
Wen-Jian
WJ
Zhang
Hong
H
Yang
Jian-She
JS
Qiu
Rong
R
Wang
Xiao
X