Identification of a new allele, HLA-DRB5*0113, through three different molecular biology techniques.
A new HLA-DRB5 allele, HLA-DRB5*0113, has been identified in an Italian patient during routine HLA typing in order to activate a bone marrow donor search. HLA typing was performed by different molecular biology techniques, and the results showed that the HLA-DRB5*0113 allele differs from HLA-DRB5*010101 allele for three nucleotide substitutions at codons 57 (GAC-->GAT; Asp) and 58 (GCT-->GAG; Ala-->Glu) of exon 2.
Garino
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E
Transplantation Immunology, San Giovanni Battista Hospital, Turin, Italy. egarino@molinette.piemonte.it
Berrino
M
M
Bertinetto
F
F
Caropreso
P
P
Chidichimo
R
R
Dametto
E
E
Fasano
M E
ME
Frisaldi
E
E
Mazzola
G
G
Tondat
F
F
Boccadoro
M
M
Bruno
B
B
Amoroso
A
A