title: Chloroplast sulfhydryl groups and the light activation of fructose-1,6-bisphosphatase.
abstract: Studies of isolated intact spinach (Spinacia oleracea L.) chloroplasts reveal that most of the available sulfhydryl groups are associated with stromal protein as opposed to a thylakoid membrane fraction under non-denaturing conditions. Increases in sulfhydryl content of approximately 50% occurred with illumination and could be correlated kinetically with a reductive activation of fructose-1,6-bisphosphatase during CO(2)-assimilation. Inhibition of linear electron flow with 3-(3,4-dichlorophenyl)-1,1-dimethylurea prevented light driven increases in both fructose-1,6-bisphosphatase activity and the relative sulfhydryl number. These results provide evidence for the operation of a reductive enzyme activating system in vivo.
authors: Biosciences Research, Sullivan Park FR-64, Corning Glass Works, Corning, New York 14831.
authors: R E
authors: RE
authors: Slovacek
authors: S
authors: S
authors: Vaughn