Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis.
Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genus Staphylococcus with the recognition sequence CCNGG.
Bens
Corina C P M
CC
Department of Medical Microbiology and Infectious Diseases, Regional Public Health Laboratory, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
Voss
Andreas
A
Klaassen
Corné H W
CH