Flowering Response of Pharbitis nil to Agents Affecting Cytoplasmic pH.
Permeant weak acids and auxins have been shown to reduce the cytosplasmic pH in several systems. Lactic, citric, formic, butyric, salicylic, parahydroxybenzoic, propionic acid, and sodium propionate inhibited the flowering response of Pharbitis nil seedlings when applied immediately before an inductive dark period. The acidic auxins IAA, indolebutyric, and alpha-naphtaleneacetic acid, as well as the nonacidic auxin alpha-naphtaleneaceteamid, also inhibited the flowering response. Inhibition was generally more pronounced with a 12-hour than with a 16-hour dark period. Salicylic acid and sodium propionate shifted the response curve of the dark period by about 2 hours. Salicyclic acid, sodium propionate, and indolebutyric acid were inhibitory when applied during the first few hours of the dark period. The permeant weak bases NH(4)Cl, procaine, and trisodium citrate enhanced the flowering response. NH(4)Cl reduced the length of the critical dark period. The inhibition of flowering by acids and auxins as well as the promotion of flowering by bases was obtained even when only the cotyledons had been treated. The inhibition of floral induction by auxins may not be dependent on their effect on the cytoplasmic pH.
Friedman
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The Hebrew University of Jerusalem, Department of Horticulture, P. O. Box 12, Rehovot 76100, Israel.
Spiegelstein
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Goldschmidt
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Halevy
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