title: Immunochemical Characterization of Nitrate Reductase Forms from Wild-Type (cv Williams) and nr(1) Mutant Soybean.
abstract: Soybean (Glycine max L. Merr.) leaves contain two forms of nitrate reductase (NR)-NAD(P)H:NR and NADH:NR. Wild-type (cv Williams), nr(1) mutant and an unrelated cultivar (Prize) were grown with either no N source or with nitrate. Crude extracts were assayed for NR activities and the enzyme forms were purified on blue Sepharose. Analyses were done by polyacrylamide gel electrophoresis and ;Western blotting' using antibodies specific for NR. NAD(P)H:NR was identified as the constitutive NR present in wild-type and Prize, but was absent from the mutant. All three soybean lines contained nitrate-inducible NADH:NR with highest activity at pH 7.5. The results showed that NAD(P)H:NR and constitutive NR were one in the same and confirmed the presence of NADH:NR with pH 7.5 optimum.
authors: Department of Chemistry State University of New York, College of Environmental Science and Forestry, Syracuse, New York 13210.
authors: P
authors: P
authors: Robin
authors: L
authors: L
authors: Streit
authors: W H
authors: WH
authors: Campbell
authors: J E
authors: JE
authors: Harper