title: Development of a CA125-mesothelin cell adhesion assay as a screening tool for biologics discovery.
abstract: Preventing peritoneal implantation of ovarian carcinoma cells could prolong patient remission and survival. CA125 is expressed on most ovarian cancer cells and was reported to be a ligand of mesothelin, a peritoneal protein. We developed a cell adhesion assay with CA125-expresser ovarian cancer cells and human mesothelin-transfected cells and we confirmed that CA125 and mesothelin mediate cell attachment. We also showed that this assay supplies a high-throughput screening system for reagents able to block CA125/mesothelin-dependent cell attachment with a sensitive quantitative readout. We finally demonstrated that a mesothelin chimeric protein and anti-CA125 antibodies block CA125/mesothelin-dependent cell attachment.
authors: Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA 98109-1024, USA. nscholle@fhcrc.org
authors: Nathalie
authors: N
authors: Scholler
authors: Barbara
authors: B
authors: Garvik
authors: Martha
authors: M
authors: Hayden-Ledbetter
authors: Toni
authors: T
authors: Kline
authors: Nicole
authors: N
authors: Urban