High speed HPLC analysis of polyamines in plant tissues.
A high speed high performance liquid chromatography (HPLC) method for the quantification of putrescine, spermidine, and spermine in biological samples is described. The dansylation is followed by a sample cleanup. The isocratic HPLC-analysis with acetonitrile:H(2)O (72:28 volume/volume) on 10 centimeter long, 3 micrometer octadecyl silica columns (3 millimeter inner diameter) takes only 4.5 minutes. By our method about 100 analyses can be done in 1 day.
Walter
H J
HJ
Lab. Plantenfysiologie, Kard. Mercierlaan 92, B-3030 Heverlee, Belgium.
Geuns
J M
JM