title: Sexual Agglutination in the Unicellular Green Alga Chlamydomonas eugametos: Identification and Properties of the Mating Type plus Agglutination Factor.
abstract: Gametes of the unicellular green alga Chlamydomonas eugametos agglutinate via their flagella. The mating type plus agglutination factor was solubilized by relatively mild treatments such as a short pH shock or an osmotic shock indicating that it is an extrinsic membrane component. It was also extracted in the nonionic detergent Triton X-100. A simple two-step procedure consisting of gel filtration over Sepharose 4B-cross-linked followed by anion exchange chromatography of the void volume yielded an electrophoretically pure preparation of a single high molecular weight glycoprotein. The agglutination factor sedimented as a 9.3 S particle (assuming a density of 1.50) in sucrose gradients. This low value, compared with the high apparent molecular weight seen during gel filtration and electrophoresis, suggests that the agglutination factor is a rod-like molecule. This was confirmed by viewing rotary-shadowed preparations in the electron microscope. A population of long slender molecules was revealed (328 +/- 20 nanometers), many of which had a knob at one end and a flexible region about one fourth of the length from the other end.
authors: Department of Plant Physiology, University of Amsterdam, Kruislaan 318, Amsterdam, The Netherlands.
authors: F M
authors: FM
authors: Klis
authors: M R
authors: MR
authors: Samson
authors: E
authors: E
authors: Touw
authors: A
authors: A
authors: Musgrave
authors: H
authors: H
authors: van den Ende