title: Fluorescein-labeled stable neurotensin derivatives.
abstract: Neurotensin(8-13) analogs containing a glycine or 5-aminovaleroyl spacer were labeled with fluorescein through formation of an N-terminal thiourea function. The receptor binding was measured in HT-29 cell cultures and showed a substantial decrease in affinity, especially for the metabolically stabilized [MeArg(9), Tle(11)] analog. Using fluorescence microscopy, the internalization of the fluorescent neurotensin analogs into HT-29 cells was observed.
authors: Organic Chemistry Department, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium.
authors: Veronique
authors: V
authors: Maes
authors: Christina
authors: C
authors: Hultsch
authors: Suzann
authors: S
authors: Kohl
authors: Ralf
authors: R
authors: Bergmann
authors: Thomas
authors: T
authors: Hanke
authors: Dirk
authors: D
authors: Tourwé