title: [An experimental study on the antitumor effects induced by fusions of dendritic cells with NCI-H460 lung cancer cells].
abstract: OBJECTIVE
  To study the antitumor effects induced by fusions of dendritic cells (DC) and NCI-H460 cells and compared with antigen pulsed DC.
  
  
  METHODS
  (1) DC were induced from human monocytes and were fused with NCI-H460 cells. Three experimental groups were set up, including group fusions cell (FC), group pulsed cell (PC) and group T cell (TC), for which T cells activated by fusions, antigen pulsed DC, and non-activated T cells were used as the effector cells respectively. The killing activity of effector cells to NCI-H460 cells were evaluated by lactate dehydrogenase method. (2) NCI-H460 cells were injected subcutaneously to BALB/c nude mice. Then 18 cancer-bearing mice were divided randomly into 3 groups, group FC, group PC and group TC. Effector cells mentioned above were inoculated subcutaneously. The tumor size and tumor weight were assessed and compared.
  
  
  RESULTS
  The killing rates to NCI-H460 cells of group FC, group PC and group TC were 43.54%, 26.57% and 3.25% respectively. Comparison of the killing activity of these 3 groups showed group FC > group PC > group TC (F = 5.47, P < 0.05). The tumor size of group FC was significantly smaller than that of group PC and group TC. The tumor weights of group FC, group PC and group TC were (1,129 +/- 123) mg, (1,709 +/- 160) mg and (3,344 +/- 288) mg respectively (F = 37.05, P < 0.01).
  
  
  CONCLUSIONS
  Fusions of DC and NCI-H460 cells could induce powerful antitumor immunity, which was more effective than antigen pulsed DC.
authors: Cancer Center, Sir Run Run Shaw Hospital, Medical College, Zhejiang University, Hangzhou 310016, China.
authors: Zai-yun
authors: ZY
authors: Zhang
authors: Jin-min
authors: JM
authors: Wu
authors: Hong-ming
authors: HM
authors: Pan