title: Inactivation of pea leaf chloroplastic and cytoplasmic glucose 6-phosphate dehydrogenases by light and dithiothreitol.
abstract: Chloroplastic and cytoplasmic forms of pea (Pisum sativum L.) leaf glucose-6-P dehydrogenase can be separated by gel electrophoresis. Both forms are found in etiolated seedlings. When light-grown plants are illuminated or when crude extracts are treated with dithiothreitol, the chloroplastic and cytoplasmic forms of this enzyme are inactivated. Michaelis-Menten kinetics are obtained for inactivation of the chloroplastic and cytoplasmic glucose-6-P dehydrogenases and for activation of two dehydrogenases involved in photosynthetic carbon metabolism with dithiothreitol. The mechanism for the activation of the two light-activated enzymes appears to be similar to the mechanism for the inactivation of the chloroplastic and cytoplasmic forms of the light-inactivated oxidative pentose phosphate enzyme glucose-6-P dehydrogenase.
authors: Department of Biological Sciences, University of Illinois at Chicago Circle, Chicago, Illinois 60680.
authors: L E
authors: LE
authors: Anderson
authors: T C
authors: TC
authors: Ng
authors: K E
authors: KE
authors: Park