title: Measurement of the Enzyme-CO(2)-Mg Form of Spinach Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase.
abstract: When the amount of activation of ribulose 1,5-bisphosphate carboxylase has been measured, two forms of the enzyme, not one, are actually determined experimentally. Only the enzyme-activator CO(2)-Mg(2+) form can bind ribulose bisphosphate for reaction with substrate CO(2) or O(2). A method is presented which measures only this catalytically active form by stabilizing it with ribulose bisphosphate just before dilution and assay in Mg(2+)-free reaction medium.
authors: Department of Biochemistry and Plant Sciences, University of Arizona, Tucson, Arizona 85721.
authors: R E
authors: RE
authors: Seftor
authors: J T
authors: JT
authors: Bahr
authors: R G
authors: RG
authors: Jensen