title: Ex vivo measurement of brain tissue viscoelasticity in postischemic brain edema.
abstract: Knowledge of the biomechanical properties of postischemic brain tissue is important for understanding the mechanisms of postischemic secondary brain tissue injury. We describe the method and results of biomechanical property measurement in ex vivo postischemic brain tissue by applying an indentation method. Mongolian gerbils were subjected to a transient unilateral hemispheric ischemia. At day 1 after ischemia, multi-parametric MRI was performed, the brain was removed under anesthesia, sliced, and kept in a container with silicone oil for the measurement. A compression probe attached to a pressure transducer was inserted to a pre-determined depth at the regions of interest and maintained at a constant speed. A pressure relaxation curve was recorded for the calculation of elasticity modulus (E) and viscosity modulus (eta) according to Maxwell-Voigt's 3-element model. One day after ischemia, E and eta decreased to 78.7% and 73.1% of the control level, respectively. This decrease corresponded to a mild decrease in apparent diffusion coefficient (ADC) and magnetization transfer ratio, and an increase in T2 value. Tissue water content increased to 105.1% of control. Microvacuolation with demyelination and axonal disruption was evident in the postischemic brain tissue.
authors: Department of Neuropathology, Tokyo Medical and Dental University, Medical Research Institute, Tokyo, Japan. t.kuroiwa.npat@mri.tmd.ac.jp
authors: T
authors: T
authors: Kuroiwa
authors: I
authors: I
authors: Yamada
authors: N
authors: N
authors: Katsumata
authors: S
authors: S
authors: Endo
authors: K
authors: K
authors: Ohno