Column-switching high-performance liquid chromatographic analysis of fluvastatin in rat plasma by direct injection.
A column-switching high-performance liquid chromatographic (HPLC) method has been developed and validated for quantification of fluvastatin in rat plasma. Plasma samples were diluted with an equal volume of mobile phase, i.e. acetonitrile-5 mM potassium phosphate buffer (pH 6.8) (15:85, v/v), and the mixture was directly injected onto the HPLC system. The analyte was enriched in a pre-treatment column, while endogenous components were eluted to waste. The analyte was then back-flushed onto an analytical column and quantified with fluorescence detection (lambdaex=305 nm; lambdaem=390 nm). The standard curve for the drug was linear in the range 0.5-100 ng mL(-1) in rat plasma. The limit of quantitation for plasma was found to be 0.5 ng mL(-1). This method has been fully validated and shown to be specific, accurate and precise. The method is simple and rapid because of a minimized sample preparation and appears to be useful for the pharmacokinetic study of fluvastatin.
Um
So Young
SY
Division of Biopharmaceutics, Department of Pharmacology, National Institute of Toxicological Research, Korea Food and Drug Administration, Nokbun-dong 5, Eunpyung-Ku, Seoul, Republic of Korea.
Jung
Sung Hee
SH
Jung
Seo Jeong
SJ
Kim
Joo Il
JI
Chung
Soo Youn
SY
Lee
Hwa Jeong
HJ
Han
Sang Beom
SB
Choi
Sun Ok
SO