Light-Induced Nuclear Synthesis of Spinach Chloroplast Fructose-1,6-bisphosphatase.
Etiolated spinach (Spinacia oleracea L. var Winter Giant) seedlings show a residual photosynthetic fructose-1,6-bisphosphatase activity, which sharply rises under illumination. This increase in activity is due to a light-induced de novo synthesis, as it has been demonstrated by enzyme labeling experiments with (2)H(2)O and [(35)S]methionine. The rise of bisphosphatase activity under illumination is strongly inhibited by cycloheximide, but not by the 70S ribosome inhibitor lincocin, which shows the nuclear origin of this chloroplastic enzyme.
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Unidad de Bioquímica Vegetal, Estación Experimental del Zaidín (CSIC), Granada, Spain.
Lázaro
J J
JJ
Gorgé
J L
JL