Structure activity relationships of monocyte chemoattractant proteins in complex with a blocking antibody.
Monocyte chemoattractant proteins (MCPs) are cytokines that direct immune cells bearing appropriate receptors to sites of inflammation or injury and are therefore attractive therapeutic targets for inhibitory molecules. 11K2 is a blocking mouse monoclonal antibody active against several human and murine MCPs. A 2.5 A structure of the Fab fragment of this antibody in complex with human MCP-1 has been solved. The Fab blocks CCR2 receptor binding to MCP-1 through an adjacent but distinct binding site. The orientation of the Fab indicates that a single MCP-1 dimer will bind two 11K2 antibodies. Several key residues on the antibody and on human MCPs were predicted to be involved in antibody selectivity. Mutational analysis of these residues confirms their involvement in the antibody-chemokine interaction. In addition to mutations that decreased or disrupted binding, one antibody mutation resulted in a 70-fold increase in affinity for human MCP-2. A key residue missing in human MCP-3, a chemokine not recognized by the antibody, was identified and engineering the preferred residue into the chemokine conferred binding to the antibody.
Reid
Carl
C
Department of Research, Biogen Idec, Inc. 12 Cambridge Center, Cambridge, MA 02142, USA.
Rushe
Mia
M
Jarpe
Matthew
M
van Vlijmen
Herman
H
Dolinski
Brian
B
Qian
Fang
F
Cachero
Teresa G
TG
Cuervo
Hernan
H
Yanachkova
Milka
M
Nwankwo
Chioma
C
Wang
Xin
X
Etienne
Natalie
N
Garber
Ellen
E
Bailly
Véronique
V
de Fougerolles
Antonin
A
Boriack-Sjodin
P Ann
PA