title: Transient receptor potential channels in essential hypertension.
abstract: OBJECTIVE
  The role of nonselective cation channels of the transient receptor potential channel (TRPC) family in essential hypertension has not yet been investigated.
  
  
  METHODS
  We studied TRPCs in 51 patients with essential hypertension and 51 age-matched and sex-matched normotensive control subjects. Calcium and gadolinium influx into human monocytes was determined using the fluorescent dye technique. TRPC expression was measured using reverse transcriptase-polymerase chain reaction and in-cell western assay. Gene silencing by small interfering RNA for specific TRPC knockdown was also performed.
  
  
  RESULTS
  We observed an increased gadolinium/calcium-influx ratio through TRPC in essential hypertensive patients compared with normotensive control subjects [cation influx ratio (mean +/- SEM), 125 +/- 14 versus 80 +/- 7%; each n = 51; P < 0.01], due to an increase of gadolinium influx in hypertensive patients compared with normotensive control subjects (48 +/- 4 versus 36 +/- 3%; each n = 51; P < 0.05). We observed a significant increase of TRPC3 and TRPC5 protein expression in essential hypertensive patients compared with normotensive control subjects (normalized TRPC3 expression, 3.21 +/- 0.59 versus 1.36 +/- 0.07; each n = 20; P < 0.01; normalized TRPC5 expression, 2.10 +/- 0.28 versus 1.40 +/- 0.52; each n = 12; P < 0.05). We used small interfering RNA for knockdown of TRPC5. The thereby reduced channel expression caused a significant attenuation of calcium and gadolinium influx.
  
  
  CONCLUSIONS
  This study points to an important role of TRPCs in essential hypertension.
authors: Med. Klinik IV, Nephrologie, Charité Campus Benjamin Franklin, Berlin, Germany.
authors: Daoyan
authors: D
authors: Liu
authors: Alexandra
authors: A
authors: Scholze
authors: Zhiming
authors: Z
authors: Zhu
authors: Katharina
authors: K
authors: Krueger
authors: Florian
authors: F
authors: Thilo
authors: Antje
authors: A
authors: Burkert
authors: Katrin
authors: K
authors: Streffer
authors: Stefan
authors: S
authors: Holz
authors: Christian
authors: C
authors: Harteneck
authors: Walter
authors: W
authors: Zidek
authors: Martin
authors: M
authors: Tepel