title: Use of solution-IEF-fractionation leads to separation of 2673 mouse brain proteins including 255 hydrophobic structures.
abstract: Analyzing complex protein mixtures on a single gel does not allow separation of many extracted proteins. Herein, we tried a prefractionation approach and mouse brain proteins were separated on a narrow pH range ZOOM-IEF Fractionator (MicroSol-IEF device) and run on two-dimensional gel electrophoresis. A total number of 2673 protein spots including 255 hydrophobic structures were successfully analyzed by mass spectrometry. This nonsophisticated approach to increase protein identification of a brain protein extract is a step forward in neurochemistry.
authors: Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
authors: Jae-Kyung
authors: JK
authors: Myung
authors: Gert
authors: G
authors: Lubec