title: Protein Synthesis in Isolated Mitochondria of Rice (Oryza sativa L.) Seedlings.
abstract: For studies of in organello mitochondrial protein synthesis in rice, Oryza sativa L., conventional surface-sterilization procedures were demonstrated to be ineffective. Because of the over-whelmingly efficient [(35)S]methionine utilization by contaminating bacteria, even "essentially bacteria-free" rice mitochondria were shown to be unsuitable for the study of in organello protein synthesis. We developed a procedure to obtain a bacteria-free preparation of rice mitochondria. Such mitochondria favored a membrane-dependent ATP-generating system over an external ATP-generating system as the energy supplement for in organello protein synthesis. Two distinct classes of [(35)S]methionine-labeled, cycloheximide-insensitive products were detected: an electrophoretically unresolved population and a set of some 22 to 27 discrete polypeptide species, each with a characteristic electrophoretic mobility and relative abundance.
authors: Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.
authors: H
authors: H
authors: Dai
authors: Y S
authors: YS
authors: Lo
authors: C Y
authors: CY
authors: Wu
authors: C L
authors: CL
authors: Tsou
authors: G S
authors: GS
authors: Hsu
authors: C G
authors: CG
authors: Chern
authors: M
authors: M
authors: Ruddat
authors: K S
authors: KS
authors: Chiang