Structural analysis of water-soluble glucans from the root of Angelica sinensis (Oliv.) Diels.
Two water-soluble glucans (designated APS-1cI and APS-1cII) were extracted from the roots of Angelica sinensis (Oliv.) Diels and further purified by anion-exchange and gel-filtration chromatography. Their molecular weights were determined to be 1.7 x 10(5) and 3.9 x 10(4)Da, respectively. The structures of the purified glucans were investigated by a combination of chemical and instrumental analysis, such as methylation analysis, periodate oxidation, GC-MS, as well as FTIR and NMR spectroscopy ((1)H, (13)C, H-H COSY, HSQC, HMBC, TOCSY and NOESY). The data obtained indicated that APS-1cI was a linear alpha-glucan composed of only (1-->6)-alpha-D-Glcp, and APS-1cII had a repeating unit consisting of (1-->4)-alpha-D-Glcp and (1-->6)-alpha-D-Glcp in a molar ratio of 4:1. Such glucans isolated from A. sinensis (Oliv.) Diels have not been previously reported.
Cao
Wei
W
Department of Pharmacology, School of Pharmacy, the Fourth Military Medical University, Xi'an, China.
Li
Xiao-Qiang
XQ
Liu
Li
L
Wang
Minchang
M
Fan
Hui-Ting
HT
Li
Chen
C
Lv
Zhengguang
Z
Wang
Xiaojing
X
Mei
Qibing
Q