title: Automatic identification of biological microorganisms using three-dimensional complex morphology.
abstract: We propose automated identification of microorganisms using three-dimensional (3-D) complex morphology. This 3-D complex morphology pattern includes the complex amplitude (magnitude and phase) of computationally reconstructed holographic images at arbitrary depths. Microscope-based single-exposure on-line (SEOL) digital holography records and reconstructs holographic images of the biological microorganisms. The 3-D automatic recognition is processed by segmentation, feature extraction by Gabor-based wavelets, automatic feature vector selection by graph matching, training rules, and a decision process. Graph matching combined with Gabor feature vectors measures the similarity of complex geometrical shapes between a reference microorganism and unknown biological samples. Automatic selection of the training data is proposed to achieve a fully automatic recognition system. Preliminary experimental results are presented for 3-D image recognition of Sphacelaria alga and Tribonema aequale alga.
authors: University of Connecticut, Electrical and Computer Engineering Department, 371 Fairfield Road, Unit 2157, Storrs, Connecticut 06269-2157, USA.
authors: Seokwon
authors: S
authors: Yeom
authors: Bahram
authors: B
authors: Javidi