title: Increase in synaptic hippocampal zinc concentration following chronic but not acute zinc treatment in rats.
abstract: Electroconvulsive seizures (ECS), one of the most effective treatments of depression, induce mossy fiber sprouting (when assayed by means of synaptic zinc method), and this indicates an increase in the synaptic zinc level in the hippocampus following such therapy. The aim of the present study was to investigate the influence of acute and chronic zinc hydroaspartate administration on the synaptic and total zinc level in the rat hippocampus. We used two methods of zinc determination: (1) zinc-selenium method, which images the pool of synaptic zinc, and (2) flame atomic absorption spectrometry, which assays the total concentration of zinc. Our results indicate that chronic (14 x 65 mg/kg), but not acute, zinc hydroaspartate administration intraperitoneally (i.p.) increases the pool of synaptic zinc in the majority of rat hippocampal layers (by 72-190%), except for the stratum moleculare and stratum radiatum CA, and perforant path DG. On the other hand, no changes were found in total hippocampal zinc level, measured by flame atomic absorption spectrometry. These data suggest that chronic zinc treatment increases the pool of synaptic zinc in the hippocampus, and this effect is similar to that observed following chronic ECS treatment. The measurement of zinc concentration in the whole hippocampus by the flame atomic absorption spectrometry method is not sensitive enough to detect such subtle alteration.
authors: Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, PL31-343 Krakow, Poland.
authors: Bernadeta
authors: B
authors: Szewczyk
authors: Magdalena
authors: M
authors: Sowa
authors: Artur
authors: A
authors: Czupryn
authors: Joanna M
authors: JM
authors: Wierońska
authors: Piotr
authors: P
authors: Brański
authors: Krystyna
authors: K
authors: Sadlik
authors: Włodzimierz
authors: W
authors: Opoka
authors: Wojciech
authors: W
authors: Piekoszewski
authors: Maria
authors: M
authors: Smiałowska
authors: Jolanta
authors: J
authors: Skangiel-Kramska
authors: Andrzej
authors: A
authors: Pilc
authors: Gabriel
authors: G
authors: Nowak