title: Isolation of nucleoli and localization of ribonucleic Acid polymerase I from soybean hypocotyl.
abstract: An effective method for the isolation of nucleoli from auxin-treated soybean (Glycine max, var. Wayne) hypocotyl was developed by polytron homogenization and sucrose gradient centrifugation. The nucleoli expressed only the alpha-amanitin-insensitive RNA synthetic activity. This activity chromatographed as RNA polymerase I on DEAE-cellulose. It appears that the plant nucleolus, like the animal nucleolus, is the site of localization for RNA polymerase I.
authors: Department of Botany, University of Georgia, Athens, Georgia 30602.
authors: C Y
authors: CY
authors: Lin
authors: T J
authors: TJ
authors: Guilfoyle
authors: Y M
authors: YM
authors: Chen
authors: J L
authors: JL
authors: Key