Repeated measurements of aperture for individual stomates.
Measurements of stomatal aperture in epidermal peels are used in a variety of physiological studies, but variability between stomates often hinders experimentation. We mount epidermal peels of Vicia faba over a microscopic grid that enables us to map the positions of individual stomates and thus measure the same individual stomates repeatedly, using a simple digitizing system. Repeated measurements of the same population of 15 stomates show much lower variability than measurements of different populations either on the same peel or on different peels.
Gorton
H L
HL
Biology Department, Trinity College, Hartford, Connecticut 06106.
Williams
W E
WE
Binns
M E
ME