Solubilization of the starch-granule-bound starch synthase of normal maize kernels.
The starch-granule-bound starch synthase from Zea mays has been solubilized with a recovery of between 50 and 84%. Chromatography of the solubilized enzyme on DEAE-Sepharose resolves two fractions of activity which may be distinguished by their response to citrate. Neither solubilized isoenzyme displays any significant activity with UDPglucose.
Macdonald
F D
FD
Department of Biochemistry and Biophysics, University of California, Davis, California 95616.
Preiss
J
J