Cytokinin Biosynthesis in Mutants of the Moss Physcomitrella patens.
Three cytokinin-over-producing mutants of the moss, Physcomitrella patens, have been shown to convert [8-(14)C]adenine to N(6)-[(14)C](Delta(2)-isopentenyl)adenine, the presence of which was confirmed by thin layer chromatography, high performance liquid chromatography, and recrystallization to constant specific radioactivity. The labeled cytokinin was detected in the culture medium within 6 hours and the tissue itself appears to contain both labeled N(6)-(Delta(2)-isopentenyl)adenine and N(6)-(Delta(2)-isopentenyl)adenosine monophosphate.
Wang
T L
TL
Department of Genetics, University of Leeds, Leeds, LS2 9JT United Kingdom.
Beutelmann
P
P
Cove
D J
DJ