title: Fucosylation of xyloglucan: localization of the transferase in dictyosomes of pea stem cells.
abstract: Microsomal membranes from elongating regions of etiolated Pisum sativum stems were separated by rate-zonal centrifugation on Renografin gradients. The transfer of labeled fucose and xylose from GDP-[(14)C] fucose and UDP-[(14)C]xylose to xyloglucan occurred mainly in dictyosomeenriched fractions. No transferase activity was detected in secretory vesicle fractions. Pulse-chase experiments using pea stem slices incubated with [(3)H]fucose suggest that xyloglucan chains are fucosylated and their structure completed within the dictyosomes, before being transported to the cell wall by secretory vesicles.
authors: Department of Biology, McGill University, 1205 Avenue du Docteur Penfield, Montréal, Québec, Canada H3A 1B1.
authors: A
authors: A
authors: Camirand
authors: D
authors: D
authors: Brummell
authors: G
authors: G
authors: Maclachlan