Isolation and characterization of a novel nuclear protein from pollen mother cells of lily.
Pollen mother cells of the lily (Lilium speciosum) were found to have a histone-H1-like protein (PMCP) not detected in other tissues. The PMCP appears from the late S-G(2) period of premeiosis and is present in mature pollen. PMCP and H1 were extracted from pollen mother cells with 5% perchloric acid and isolated by reverse-phase high-performance liquid chromatography. The amino acid composition of PMCP differs from that of somatic H1. However, PMCP is similar to H1t in mammalian testis with regard to amino acid composition.
Sasaki
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Institute of Biological Sciences, University of Tsukuba, Ibaraki 305, Japan.
Yasuda
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Ohba
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Harada
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H