Characterization of a cytosolic aconitase in higher plant cells.
Protoplasts obtained from sycamore (Acer pseudoplatanus) cell suspensions were found to be highly intact. If the protoplasts were taken up and expelled through a fine nylon mesh, all the protoplasts were ruptured leaving the fragile amyloplasts largely intact. Aconitase hydratase (citrate [isocitrate] hydro-lyase, EC 4.2.1.3) activity of sycamore cells was associated with two protein fractions, one present in the cytosol while the second is of mitochondrial origin. Chromatography on DEAE-trisacryl did not separate the aconitase hydratase isoenzymes. EPR studies established that both isoenzymes exhibited an EPR signal at g = 2.03 once oxidized.
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Laboratoire de Physiologie Cellulaire Végétale, U.A. CNRS No. 576, DRF, CENG and USMG, 85 X, F-38041 Grenoble-Cédex, France.
Nishimura
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Gaillard
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Douce
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