Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin.
We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%).
Pomba
Constança
C
CIISA, Faculty of Veterinary Medicine, Universidade Técnica de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal. cpomba@fmv.utl.pt
Mendonça
Nuno
N
Costa
Marta
M
Louro
Deolinda
D
Baptista
Bruno
B
Ferreira
Mónica
M
Correia
José Duarte
JD
Caniça
Manuela
M