Influence of the spectrophotometric detection mode on the separation performance of systems with monolithic capillary columns: on-column and external-cell detection modes.
Poly(butyl methacrylate) monolithic columns were prepared by thermally initiated radical polymerization in fused-silica capillaries of 320 microm i.d. The prepared monolithic columns were tested by capillary liquid chromatography (CLC) combined with a UV-VIS spectrophotometric detector. The influence of the detection configuration (i.e., on-column and external-cell detection modes) on the performance of the chromatographic system was investigated. In the on-column detection mode within the monolith, the detection window was located inside the column section filled with the monolith. With the on-column detection configuration after the monolith, the detection window was positioned just behind the column section containing the monolith. Using the external-cell detection mode, an additional detection capillary, provided with a detection window defining the external-cell, was connected to the monolithic capillary column. These detection modes were critically compared in terms of the principal chromatographic parameters of the system involving the prepared monolithic capillary columns.
Grafnetter
Jan
J
Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague, Czech Republic.
Coufal
Pavel
P
Suchánková
Jana
J
Stulík
Karel
K