Pathogenesis-related proteins and polyamines in a developmental mutant of tomato, epinastic.
The polyamine level and the accumulation of pathogenesis-related (PR) proteins were studied in the ethylene overproducing Epinastic (Epi) tomato (Lycopersicon esculentum Mill.) mutant, as compared with its parent, cv VFN8. Neither a decreased putrescine level nor an enhanced production of PR proteins were detected in Epi, contrary to what could be expected from our previous studies (JM Bellés, J Carbonell, V Conejero [1991] Plant Physiol 96: 1053-1059). However, treatment with the ethylene-releasing compound 2-chloroethylphosphonic acid (ethephon) or silver nitrate at high doses induced a decrease in putrescine content and an enhancing of the synthesis of PR proteins in Epi as ascertained by immunoblot analysis using antisera raised against Rutgers tomato PR proteins.
Bellés
J M
JM
Laboratorio de Bioquímica y Biología Molecular, Departamento de Biotecnología, Universidad Politécnica de Valencia, 46022-Valencia, Spain.
Tornero
P
P
Conejero
V
V