title: Ultrasensitive DNA chip: gene expression profile analysis without RNA amplification.
abstract: We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis.
authors: New Frontiers Research Laboratories, Toray Industries, Inc., Kanagawa 248-8555. Kunihisa_nagino@nts.toray.co.jp
authors: Kunihisa
authors: K
authors: Nagino
authors: Osamu
authors: O
authors: Nomura
authors: Yuki
authors: Y
authors: Takii
authors: Akira
authors: A
authors: Myomoto
authors: Makiko
authors: M
authors: Ichikawa
authors: Fumio
authors: F
authors: Nakamura
authors: Masashi
authors: M
authors: Higasa
authors: Hideo
authors: H
authors: Akiyama
authors: Hitoshi
authors: H
authors: Nobumasa
authors: Satoshi
authors: S
authors: Shiojima
authors: Gozoh
authors: G
authors: Tsujimoto