title: Improving the predictive value of the hypoosmotic swelling test in humans.
abstract: Using a combined hypoosmotic swelling-eosin (HOS-E) technique in human semen samples, we evaluated the frequency of dead swollen spermatozoa (dHOS) after 10 and 30 minutes of incubation, the correlation between total HOS-reactive (tHOS) and viable HOS-reactive (vHOS) spermatozoa with other seminal parameters, and the possibility that dead spermatozoa react to HOS. We obtained the following results: [1] some dead spermatozoa swell under hypoosmotic conditions and [2] HOS-E results correlate strongly with other seminal parameters. We recommend that HOS be performed after 10 minutes of incubation because [1] the increase in the incubation time enhances the percentage of dHOS, [2] there are no differences in vHOS percentages between 10 and 30 minutes, and [3] correlation coefficients between vHOS and tHOS with other parameters are very similar at 10 or 30 minutes of incubation.
authors: Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors: Ana C
authors: AC
authors: Martini
authors: Rosa I
authors: RI
authors: Molina
authors: Daniel
authors: D
authors: Estofán
authors: Andrea
authors: A
authors: Tissera
authors: Rubén D
authors: RD
authors: Ruiz
authors: Marta Fiol
authors: MF
authors: de Cuneo