title: Tyrosine and Phenylalanine Ammonia Lyase Activities during Shoot Initiation in Tobacco Callus Cultures.
abstract: Both phenylalanine ammonia lyase and tyrosine ammonia lyase were detected in tobacco (Nicotiana tabacum L. Wisconsin 38) callus. The enzymes were separated from each other by Sephadex G-200 column chromatography. Increased activity of tyrosine ammonia lyase was observed during culture of tobacco callus under shoot-forming conditions, while activity of phenylalanine ammonia lyase increased during culture under non-organ-forming conditions. Confirmation of these findings was obtained by examining the incorporation of [(14)C]tyrosine and [(14)C]phenylalanine into p-coumarate and trans-cinnamate, respectively.
authors: Plant Physiology Research Group, Department of Biology, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
authors: L D
authors: LD
authors: Beaudoin-Eagan
authors: T A
authors: TA
authors: Thorpe