title: Rapid development of polyclonal antisera against infectious salmon anaemia virus and its optimization and application as a diagnostic tool.
abstract: Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a drawback in its usefulness in other tests. This study describes the development and optimization of a polyclonal antiserum against infectious salmon anaemia virus, including a method of separating virus from cell culture components within culture supernatant. The antiserum was subsequently optimized for use in a variety of immunological diagnostic tests, including IFAT and an alkaline phosphatase-based immunoassay, and Western blot.
authors: FRS Marine Laboratory, Aberdeen, UK.
authors: S J
authors: SJ
authors: McBeath
authors: L M
authors: LM
authors: Ellis
authors: P F
authors: PF
authors: Cook
authors: L
authors: L
authors: Wilson
authors: K L
authors: KL
authors: Urquhart
authors: I R
authors: IR
authors: Bricknell