title: Transcription regulation in NIH3T3 cell clones resistant to diethylmaleate-induced oxidative stress and apoptosis.
abstract: To investigate the molecular mechanisms underlying the induction of cell resistance to oxidative stress, NIH3T3 cell clones (NIH-DEM clones) were isolated and selected for their ability to survive the exposure to diethylmaleate (DEM), a glutathione-depleting agent. The oxidative stress-resistant phenotype of these clones is stable for at least 1 month in the absence of DEM, and includes the resistance also to other apoptosis-inducing stimuli. The expression profile of several antioxidant genes was examined in four of the DEM-resistant clones in the presence and in absence of DEM. The response to the acute exposure to DEM is similar in wild type and DEM-resistant cells, with the exception of the glutathione-S-transferase alpha1 gene, whose expression is highly induced in NIH-DEM clones. However, in the absence of an acute stress, the expression of some genes is higher in DEM-resistant clones than in wild-type cells and the gene expression profile significantly varies among the clones. In particular, glutathione-S-transferase alpha1 and cystine/glutamate transporter mRNAs are increased in NIH-DEM-12. In these cells, the promoters of the two genes drive a stronger transcription than in wild-type cells, and this appears to be dependent on the transcription factor Nrf2.
authors: Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Napoli, Italy.
authors: Raffaella
authors: R
authors: Faraonio
authors: Paola
authors: P
authors: Vergara
authors: Domenico Di
authors: DD
authors: Marzo
authors: Maria
authors: M
authors: Napolitano
authors: Tommaso
authors: T
authors: Russo
authors: Filiberto
authors: F
authors: Cimino