A user-friendly PC-based data acquisition and analysis system for respirometric investigations.
We have developed an easy-to-use computer-based system for recording, displaying, storing and analyzing signals generated by Clark-type oxygen electrodes. A user-friendly interface of Windows-based program BioMed significantly increases the productivity of investigations. It allows to process, control, present and archive the experimental data in real time. A 12-bit analog-to-digital-converter, analog and digital filters, a possibility to zoom the obtained respiratory curves and calculation of the respiration rates by a linear regression method increase the resolution of the estimated oxygen consumption rates. The new system enables to register even small changes, such as 3-5 ngatoms O/min, in respiration rates of biological objects -- enzymes, mitochondria and permeabilized muscle fibers. The system has been developed and is regularly used for the respirometric investigations at the Laboratory of Biochemistry, Institute for Biomedical Research, Kaunas University of Medicine.
Kopustinskas
Audris
A
Faculty of Electrical and Control Engineering, Kaunas University of Technology, Studentu st. 50-303, LT-51368 Kaunas, Lithuania. audris.kopustinskas@ktu.lt
Adaskevicius
Rimas
R
Krusinskas
Andrius
A
Kopustinskiene
Dalia M
DM
Liobikas
Julius
J
Toleikis
Adolfas
A