Regulation by the cAMP cascade of oxygen free radical balance in mammalian cells.
A study is presented of the effect of the cAMP cascade on oxygen metabolism in mammalian cell cultures. Serum-starvation of the cell cultures resulted in depression of the forward NADH-ubiquinone oxidoreductase activity of complex I, decreased content of glutathione, and enhancement of the cellular level of H2O2. Depressed transcription of cytosolic Cu/Zn-SOD 1, mitochondrial glutathione peroxidase and catalase was also observed. Activation of the cAMP cascade reversed the depression of the activity of complex I and the accumulation of H2O2. The effect of cAMP involved the cAMP-dependent protein kinase.
Bellomo
F
F
Department of Medical Biochemistry, Biology and Physics, University of Bari, Bari, Italy.
Piccoli
C
C
Cocco
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T
Scacco
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S
Papa
F
F
Gaballo
A
A
Boffoli
D
D
Signorile
A
A
D'Aprile
A
A
Scrima
R
R
Sardanelli
A M
AM
Capitanio
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N
Papa
S
S