ETV4 and Myeov knockdown impairs colon cancer cell line proliferation and invasion.
We have identified novel colorectal cancer-associated genes using NCBI's UNIGENE cDNA libraries. Colon cancer libraries were examined using Digital Differential Display and disease-associated genes were selected. Among these were ETV4 and MYEOV, novel colorectal cancer-associated genes. Samples of matched normal and neoplastic colon were obtained from human subjects and gene expression was quantified using real-time PCR. ETV4 gene expression was significantly increased in colonic neoplasia in comparison to matched normal colonic tissue (p<0.05). Myeov expression was also increased in colon neoplasia in comparison to matched normal tissue. The effect of siRNA-mediated knockdown of ETV4 and Myeov on cell proliferation and invasion was assessed. ETV4 knockdown resulted in a 90% decrease in cell proliferation (p<0.05) and a 67% decrease in cell invasion. Myeov knockdown resulted in a 48% decrease in cell proliferation (p<0.05) and a 36% decrease in cell invasion. These data suggest that ETV4 and Myeov may provide novel targets for therapeutic intervention.
Moss
Alan C
AC
Conway Institute of Biomolecular and Biomedical Research, UCD, Ireland. amoss@bidmc.harvard.edu
Lawlor
Garrett
G
Murray
David
D
Tighe
Dónal
D
Madden
Stephen F
SF
Mulligan
Anne-Marie
AM
Keane
Conor O
CO
Brady
Hugh R
HR
Doran
Peter P
PP
MacMathuna
Padraic
P