Effect of pH on IAA Uptake by Maize Root Segments.
The uptake of [5-(3)H]indoleacetic acid (IAA) by Zea mays L. root segments involves nonsaturable and saturable processes. The pH optimum of the saturable component was found to be 5.0. The proton ionophore carbonylcyanide p-trifluoromethoxyphenylhydrazone inhibited at 100 micromolar the saturable component of IAA uptake but had no effect on non-saturable uptake. This indicates that the saturable component of IAA uptake is dependent on the proton gradient across the plasmalemma. The high level of proton extrusion in the elongation zone of the root will stimulate nonsaturable and saturable uptake of IAA in that zone.
Martin
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Institute of Plant Biology and Physiology, Biology Building, University of Lausanne, 1015 Lausanne, Switzerland.
Pilet
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