A simple manual rosetting method for absolute CD4+ lymphocyte counting in resource-limited countries.
A CD4 monoclonal antibody which reacts with CD4+ lymphocytes but does not react significantly with monocytes was generated and used to develop a simple method for CD4 count. In a comparison with standard flow cytometry, high correlation was obtained. The developed method can be an alternative to flow cytometry for resource-limited countries.
Nouanthong
Phonethipsavanh
P
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Pata
Supansa
S
Sirisanthana
Thira
T
Kasinrerk
Watchara
W