title: Appearance of Membrane-bound Iron-Sulfur Centers and the Photosystem I Reaction Center during Greening of Barley Leaves.
abstract: Dark-grown barley (Hordeum vulgare) etioplasts were examined for their content of membrane-bound iron-sulfur centers by electron paramagnetic resonance spectroscopy at 15K. They were found to contain the high potential iron-sulfur center characterized (in the reduced state) by an electron paramagnetic resonance g value of 1.89 (the "Rieske" center) but did not contain any low potential iron-sulfur centers. Per mole of cytochrome f, dark-grown etioplasts and fully developed chloroplasts had the same content of the Rieske center. During greening of etioplasts under continuous light, low potential bound iron-sulfur centers appear. In addition, the photosystem I reaction center, as measured by the photooxidation of P700 at 15K, also became functional; during greening the appearance of a photoreducible low potential iron-sulfur center paralleled the appearance of P700 photoactivity.These findings indicate the close association of the low potential iron-sulfur centers with the photosystem I reaction center; they also support the concept that the development of stable charge separation in the photosystem I reaction center requires, in addition to P700, a low potential iron-sulfur center.
authors: Department of Cell Physiology, University of California, Berkeley, California 94720.
authors: B G
authors: BG
authors: Baltimore
authors: R
authors: R
authors: Malkin