title: Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay.
abstract: The recombinant antigen obtained by cloning and expressing two IBV nucleocapsid protein fragments (143-414 aa, 281-414 aa) in Escherichia coli was used for the detection of avian infectious bronchitis virus (IBV) specific antibodies in chicken sera by the indirect ELISA (rNpIBV-ELISA). As a result of testing 1524 serum samples the diagnostic sensitivity and specificity of rNpIBV-ELISA when comparing those of the routine whole IBV ELISA have been shown to be 93.81% and 87.36%, respectively. The agreement value was 91.5%.
authors: FGI Federal Centre for Animal Health (FGI ARRIAH), Vladimir 600901, Russia. nlugovskaya@email.ru
authors: N N
authors: NN
authors: Lugovskaya
authors: A V
authors: AV
authors: Scherbakov
authors: A S
authors: AS
authors: Yakovleva
authors: M A
authors: MA
authors: Tsyvanyuk
authors: N S
authors: NS
authors: Mudrak
authors: V V
authors: VV
authors: Drygin
authors: A V
authors: AV
authors: Borisov