Monoclonal antibodies to 13-deoxy-gibberellins.
The production and characterization of two high affinity rat monoclonal antibodies to 13-deoxy-gibberellins is described. Hybrid myelomas were derived from rats immunized with an immunogenic keyhole limpet hemocyanin-gibberellin conjugate, linked at carbon-3 to gibberellin A(4) via a hemisuccinate bridge. The selected monoclonal antibodies were characterized by a competitive radioimmunoassay.
Knox
J P
JP
Monoclonal Antibody Centre, AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station Babraham, Cambridge CB2 4AT, U.K.
Beale
M H
MH
Butcher
G W
GW
Macmillan
J
J