Pigment analysis of chloroplast pigment-protein complexes in wheat.
Pigment-protein complexes separated from wheat (Triticum aestivum L. selection ND96-25 by two gel electrophoresis techniques were analyzed by high-performance liquid chromatography for chlorophylls and carotenoids. The two techniques are compared, and pigment analyses are given for the major reaction centers and light-harvesting complexes. Reaction centers contain mostly chlorophyll a, carotene, and lutein, whereas light-harvesting complexes contain chlorophyll a, chlorophyll b, lutein, and neoxanthin. The amounts of violaxanthin are variable.
Eskins
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Northern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Peoria, Illinois 61604.
Duysen
M E
ME
Olson
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L