title: ETV4 and Myeov knockdown impairs colon cancer cell line proliferation and invasion.
abstract: We have identified novel colorectal cancer-associated genes using NCBI's UNIGENE cDNA libraries. Colon cancer libraries were examined using Digital Differential Display and disease-associated genes were selected. Among these were ETV4 and MYEOV, novel colorectal cancer-associated genes. Samples of matched normal and neoplastic colon were obtained from human subjects and gene expression was quantified using real-time PCR. ETV4 gene expression was significantly increased in colonic neoplasia in comparison to matched normal colonic tissue (p<0.05). Myeov expression was also increased in colon neoplasia in comparison to matched normal tissue. The effect of siRNA-mediated knockdown of ETV4 and Myeov on cell proliferation and invasion was assessed. ETV4 knockdown resulted in a 90% decrease in cell proliferation (p<0.05) and a 67% decrease in cell invasion. Myeov knockdown resulted in a 48% decrease in cell proliferation (p<0.05) and a 36% decrease in cell invasion. These data suggest that ETV4 and Myeov may provide novel targets for therapeutic intervention.
authors: Conway Institute of Biomolecular and Biomedical Research, UCD, Ireland. amoss@bidmc.harvard.edu
authors: Alan C
authors: AC
authors: Moss
authors: Garrett
authors: G
authors: Lawlor
authors: David
authors: D
authors: Murray
authors: Dónal
authors: D
authors: Tighe
authors: Stephen F
authors: SF
authors: Madden
authors: Anne-Marie
authors: AM
authors: Mulligan
authors: Conor O
authors: CO
authors: Keane
authors: Hugh R
authors: HR
authors: Brady
authors: Peter P
authors: PP
authors: Doran
authors: Padraic
authors: P
authors: MacMathuna