Formation of adenosine 5'-phosphorofluoridate and the assay of adenyl cyclase in barley seeds.
Barley seed (Hordeum vulgare L.) homogenates contain an apparent enzymatic activity which catalyzes the synthesis of adenosine 5'-phosphorofluoridate from magnesium-adenosine 5'-triphosphate and sodium fluoride. Formation of this compound may interfere with some adenyl cyclase assays which use fluoride as a component of the incubation medium. Neither adenyl cyclase activity nor endogenous adenosine 3': 5'-monophosphate was detected in barley seed homogenates or extracts.
Alvarez
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Institute of Biological Sciences, Syntex Research, Palo Alto, California 94304.