title: The Binding of Indole-3-acetic Acid and 3-Methyleneoxindole to Plant Macromolecules.
abstract: Homogenates of pea (Pisum sativum L., var. Alaska) seedlings exposed to (14)C-indole-3-acetic acid or (14)C-3-methyleneoxindole, an oxidation product of indole-3-acetic acid, were extracted with phenol. In both cases 90% of the bound radioactivity was found associated with the protein fraction and 10% with the water-soluble, ethanol-insoluble fraction. The binding of radioactivity from (14)C-indole-3-acetic acid is greatly reduced by the addition of unlabeled 3-methyleneoxindole as well as by chlorogenic acid, an inhibitor of the oxidation of indole-3-acetic acid to 3-methyleneoxindole. Chlorogenic acid does not inhibit the binding of (14)C-3-methyleneoxindole. The labeled protein and water-soluble, ethanol-insoluble fractions of the phenol extract were treated with an excess of 2-mercaptoethanol. Independently of whether the seedlings had been exposed to (14)C-indole-3-acetic acid or (14)C-3-methyleneoxindole, the radioactivity was recovered from both fractions in the form of a 2-mercaptoethanol-3-methyleneoxindole adduct. These findings indicate that 3-methyleneoxindole is an intermediate in the binding of indole-3-acetic acid to macromolecules.
authors: College of Medicine, University of California, Irvine, California 92664.
authors: P S
authors: PS
authors: Basu
authors: V
authors: V
authors: Tuli