title: [Influence of LY294002 on sperm motility in asthenozoospermia patients in vitro].
abstract: OBJECTIVE
  To investigate the effects of LY294002, a phosphatidylinositol 3-kinase inhibition, on sperm motility in asthenozoospermia patients in vitro, and further analyze the possible molecular mechanism.
  
  
  METHODS
  Sperm aseptically obtained by masturbation and prepared by swim-up technique from 10 patients with asthenozoospermia and 10 healthy fertile men were incubated with different concentrations of LY294002. Measurements of motility were carried out at 10, 30 and 60 min in all specimens by CASA.
  
  
  RESULTS
  The sperm in asthenozoospermia patients treated with LY294002 showed a significant increase in sperm progressive motility, the percentage of motile cells, VSL and VAP.
  
  
  CONCLUSIONS
  LY294002 can enhance the motility of sperm in asthenozoospermia patients in vitro.
authors: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. laseryang2003@163.com
authors: Jun
authors: J
authors: Yang
authors: Ji-hong
authors: JH
authors: Liu
authors: Xiao-xin
authors: XX
authors: Gong
authors: Tao
authors: T
authors: Wang
authors: Jun
authors: J
authors: Chen
authors: Zhi
authors: Z
authors: Chen