title: Associations between RAS Gene polymorphisms, environmental factors and hypertension in Mongolian people.
abstract: OBJECTIVE
  To explore the association between RAS system genes (AGT, ACE and AT(1)R) polymorphisms, environmental factors and hypertension in Mongolian people.
  
  
  METHODS
  On the basis of cross-sectional study, a case-control study with 299 hypertensives and 281 nomotensives was conducted, and the conditions of environmental factors were acquired by questionnaire. Serum lipid and insulin were detected by using biochemical experiments. Six single nucleotide polymorphisms of RAS system were genotyped by polymerase chain reaction/restriction fragment length polymorphism and polymerase chain reaction/single strand conformation polymorphism.
  
  
  RESULTS
  Overweight or obesity, high serum TG and insulin resistance were risk factors of hypertension by single factor analysis. All the RAS genotype distributions were compatible with Hardy-Weinberg expectations. There were no significant differences to be found between cases and controls for genotype frequencies or allele frequencies of the six polymorphisms of RAS system, except in men group, OR value of men carried ACE ID+DD genotype vs. men carried II genotype was 2.20 (95%CI 1.21-4.02), and OR of people who carried both ACE ID (or DD) and AGT M235T MT (or MM) vs. people with both ACE ID (or DD) and AGT M235T TT was 1.59 (95%CI 1.06-2.38).
  
  
  CONCLUSIONS
  Overweight or obesity, dyslipidemia and insulin resistance were risk factors of hypertension in Mongolian people. ACE gene ID+DD genotype was the risk factor of hypertension in men group. People who carried both ACE ID (or DD) and AGT M235T MT (or MM) had more risk to have hypertension.
authors: Xiamen Maternal and Child Care Hospital, Xiamen, 361003, China.
authors: Wang
authors: W
authors: Gui-yan
authors: Wang
authors: W
authors: Yan-hua
authors: Xu
authors: X
authors: Qun
authors: Tong
authors: T
authors: Wei-jun
authors: Gu
authors: G
authors: Ming-ling
authors: Wang
authors: W
authors: Jian
authors: Fang
authors: F
authors: Ming-wu
authors: Zhang
authors: Z
authors: Yong-hong