title: Evidence That More than 90% of beta-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus.
abstract: Plasmid DNA harboring the beta-glucuronidase (GUS) gene, coated on gold particles, was delivered into cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells using a pneumatic particle gun. Cytological analyses of intracellular location of the introduced gold particles before and after GUS expression assay indicated that more than 90% of GUS-expressing cells after bombardment received a DNA-coated particle in their nucleus.
authors: Department of Biological Sciences, Faculty of Science, Hiroshima University, Hiroshima 730, Japan.
authors: T
authors: T
authors: Yamashita
authors: A
authors: A
authors: Iida
authors: H
authors: H
authors: Morikawa