title: Degradation of argininosuccinate lyase by a protease synthesized in soybean cell suspension cultures.
abstract: Suspension cultures of soybean (Glycine max L.) were shown to contain protease activity which could be inhibited by the addition of protease inhibitors such as p-hydroxymercuribenzoate and ethylenediaminetetraacetic acid. The use of these inhibitors, coupled with studies of the rate of degradation of argininosuccinate lyase (argininosuccinate-lyase = l-arginino-succinate arginine-lyase, EC 4.3.2.1) in extracts of cell cultures grown for 24 hours led to the hypothesis that a metal-dependent protease is synthesized by the cells after 24 hours of growth, to remove the lyase enzyme.
authors: Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors: P D
authors: PD
authors: Shargool