title: Isolation and sequencing of a temperate transducing phage for Pasteurella multocida.
abstract: A temperate bacteriophage (F108) has been isolated through mitomycin C induction of a Pasteurella multocida serogroup A strain. F108 has a typical morphology of the family Myoviridae, presenting a hexagonal head and a long contractile tail. F108 is able to infect all P. multocida serogroup A strains tested but not those belonging to other serotypes. Bacteriophage F108, the first P. multocida phage sequenced so far, presents a 30,505-bp double-stranded DNA genome with cohesive ends (CTTCCTCCCC cos site). The F108 genome shows the highest homology with those of Haemophilus influenzae HP1 and HP2 phages. Furthermore, an F108 prophage attachment site in the P. multocida chromosome has been established to be inside a gene encoding tRNA(Leu). By using several chromosomal markers that are spread along the P. multocida chromosome, it has been demonstrated that F108 is able to perform generalized transduction. This fact, together with the absence of pathogenic genes in the F108 genome, makes this bacteriophage a valuable tool for P. multocida genetic manipulation.
authors: Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona, Edifici C, Bellaterra, 08193 Barcelona, Spain.
authors: Susana
authors: S
authors: Campoy
authors: Jesús
authors: J
authors: Aranda
authors: Gerard
authors: G
authors: Alvarez
authors: Jordi
authors: J
authors: Barbé
authors: Montserrat
authors: M
authors: Llagostera