title: Effect of high temperature on calcium uptake by suspension-cultured pear fruit cells.
abstract: Uptake of Ca(2+) by suspension-cultured pear (Pyrus communis L. cv Passe Crassane) cells and protoplasts was significantly enhanced by exposure to 38 degrees C compared to 25 degrees C. The increased uptake was specific for Ca(2+) and was not due to cell wall binding. Tissues pretreated at 38 degrees C showed increased uptake even upon return to 25 degrees C. Treatment with carbonylcyanide m-chlorophenylhydrazone, salicylhydroxamic acid + KCN, or arsenite also increased Ca(2+) content of cells. Results are discussed with regard to membrane permeability changes, the cellular control of Ca(2+), and heat treatments used to inhibit softening of fruit during postharvest storage.
authors: Division of Horticulture and Processing, Department of Scientific and Industrial Research, Private Bag, Auckland, New Zealand.
authors: J D
authors: JD
authors: Klein
authors: I B
authors: IB
authors: Ferguson