Immunophenotypic characterization of peripheral blast cells in a leukemic miniature pig.
The health status of a 4-year-old female, dd-haplotype miniature pig deteriorated rapidly, so the animal finally had to be euthanized because of poor clinical condition. Necropsy revealed a massive leukocytic infiltration in the parenchymatous organs of the abdominal cavity. On hematologic cell counting, severe leukocytosis (69.3 x 10(9) cells/liter) and high-grade basophilia (6.9 x 10(9) cells/liter) were evident. Cytologic examination, as well as analysis of expression of leukocyte differentiation antigens by means of flow cytometry, classified blasts, which accounted for about 22% of leukocytes, as biphenotypic cells co-expressing the myeloid marker SWC3 (CD172a) and the lymphoid markers CD5 and CD25. Hematologic features resembled those seen in humans with chronic myeloid leukemia at blast phase.
Sipos
W
W
Clinic for Swine, Department for Farm Animals and Herd Management, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna, Austria. wolfgang.sipos@vu-wien.ac.at
Gerner
W
W
Schilcher
F
F
Leeb
C
C
Groiss
S
S
Miller
I
I
Saalmüller
A
A
Schmoll
F
F
Schwendenwein
I
I