Hyaluronan biodegradable scaffold for small-caliber artery grafting: preliminary results in an animal model.
OBJECTIVE
To evaluate a new Hyaluronan-based graft.


METHODS
Hyaluronan-based grafts (HYAFF 11trade mark tube, diameter 2 mm, length 1.5 cm) were implanted in an end-to-end fashion in the abdominal aorta of 15 rats. Histology, immunohistochemistry and electron microscopy were used to evaluate the results at 7, 21, and 90 days.


RESULTS
At day 7, new tissue was observed in the graft coming from both the proximal and distal ends of the aorta. The luminal surface of the regenerating tissue was covered by endothelial cells (CD34(+), VEGFR-2(+), vWF(+)). At day 21, regenerating tissue joined at the centre of the tube. The neo-vessel was formed by smooth muscle cells (Myosin Light Chain Kinase) as well as elastic, and collagen fibres. At day 90 a stable artery segment was formed and the biomaterial was almost completely degraded. Infiltration of neutrophils and lymphocytes was not observed. All animals survived the observation period and there were no signs of stenoses or aneurysms.


CONCLUSIONS
The hyaluronan-based graft allowed complete regeneration of a newly formed vascular tube in which all the cellular and extracellular components are present and organized in a well defined architecture similar to native artery.
Lepidi
S
S
Department of Vascular Surgery, University of Padova, Padova, Italy.
Grego
F
F
Vindigni
V
V
Zavan
B
B
Tonello
C
C
Deriu
G P
GP
Abatangelo
G
G
Cortivo
R
R