title: A paramagnetic contrast agent with myeloperoxidase-sensing properties.
abstract: Myeloperoxidase (MPO) is an essential component of inflammatory response in norm and pathology. With an ultimate goal of non-invasive imaging of MPO we used a gadolinium-chelating bis(5-hydroxytrytamide) derivative of diethylenetetraamine pentaacetic acid (L1-Gd3+ salt) as a paramagnetic sensor of enzymatic activity. We tested whether L1-Gd3+ is active in reducing the oxidized form of myeloperoxidase, generated as a result of hydrogen peroxide reduction by the enzyme. We expected that upon activation by MPO/H2O2 L1-Gd3+ would not only oligomerise but also bind to other macromolecules present in the media and that the overall process will give rise to a net T1-weighted MRI signal increase.
authors: University of Massachusetts Medical School, 55 Lake Avenue, Worcester, Massachusetts 01655, USA.
authors: Manel
authors: M
authors: Querol
authors: John W
authors: JW
authors: Chen
authors: Alexei A
authors: AA
authors: Bogdanov