Indirect somatic embryogenesis in cassava for genetic modification purposes.
In cassava both direct and indirect somatic embryogenesis is described. Direct somatic embryogenesis starts with the culture of leaf explants on Murashige and Skoog (MS) medium supplemented with auxins. Somatic embryos undergo secondary somatic embryogenesis when cultured on the same medium. Indirect somatic embryogenesis is initiated by subculture of directly induced embryogenic tissue on auxin-supplemented medium with Gresshoff and Doy salts and vitamins. A very fine friable embryogenic callus (FEC) is formed after a few rounds of subculture and stringent selection. This FEC is maintained by subculture on auxin supplemented medium. Lowering of the auxin concentration allows the FEC to form mature somatic embryos that develop into plants when transferred to a cytokinin-supplemented medium.
Raemakers
Krit
K
Laboratory of Plant Breeding, Graduate School of Experimental Plant Sciences, Wageningen University, The Netherlands.
Pereira
Isolde
I
van Putten
Herma Koehorst
HK
Visser
Richard
R