[Influence of LY294002 on sperm motility in asthenozoospermia patients in vitro].
OBJECTIVE
To investigate the effects of LY294002, a phosphatidylinositol 3-kinase inhibition, on sperm motility in asthenozoospermia patients in vitro, and further analyze the possible molecular mechanism.


METHODS
Sperm aseptically obtained by masturbation and prepared by swim-up technique from 10 patients with asthenozoospermia and 10 healthy fertile men were incubated with different concentrations of LY294002. Measurements of motility were carried out at 10, 30 and 60 min in all specimens by CASA.


RESULTS
The sperm in asthenozoospermia patients treated with LY294002 showed a significant increase in sperm progressive motility, the percentage of motile cells, VSL and VAP.


CONCLUSIONS
LY294002 can enhance the motility of sperm in asthenozoospermia patients in vitro.
Yang
Jun
J
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. laseryang2003@163.com
Liu
Ji-hong
JH
Gong
Xiao-xin
XX
Wang
Tao
T
Chen
Jun
J
Chen
Zhi
Z