Platelet-activating factor-induced NF-kappaB activation enhances VEGF expression through a decrease in p53 activity.
We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Transfected p53 increased p53RE promoter activity, which was completely inhibited by NF-kappaB subunits, indicating that cross-regulation occurs between NF-kappaB and p53. PAF-induced increase in VEGF expression was correlated with decreased p53 activity. These data suggest that NF-kappaB-dependency of the PAF-induced increase in VEGF expression is due to decreased p53 activity, which is reciprocally regulated by increased NF-kappaB activity.
Ko
Hyun-Mi
HM
Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University, Kwangju 500-757, Republic of Korea.
Jung
Hae Hyun
HH
Seo
Kook Heon
KH
Kang
Yeong-Rim
YR
Kim
Han-A
HA
Park
Sung Jun
SJ
Lee
Hern-Ku
HK
Im
Suhn-Young
SY