title: Control of apatite crystal growth by the co-operative effect of a recombinant porcine amelogenin and fluoride.
abstract: Recently, we used native amelogenins extracted from developing pig enamel to examine the combined effect of fluoride and amelogenins on the growth of octacalcium phosphate (OCP) and apatite crystals. The purpose of the present study was to investigate this combined effect using a highly purified recombinant amelogenin. We applied porcine amelogenin (rP172) and fluoride in a dual-membrane system as a model for tooth enamel formation. The combination of rP172 and fluoride in this system resulted in the formation of rod-like apatite crystals. On the other hand, without fluoride, rod-like OCP crystals of a comparable size were formed, and rather large hexagonal prisms of mixed crystals of OCP and apatite grew without amelogenins. Thus, highly purified and homogeneous recombinant amelogenin, in co-operation with F, regulated the mineral phase, habit, and size of crystals in the same manner as the extracted heterogeneous porcine amelogenins. We suggest that in both cases the control over the crystal phase and morphology was a direct effect of amelogenin protein serving as a scaffold for apatite mineralization.
authors: Dental Materials Science, Asahi University School of Dentistry, Gifu, Japan. iijima@dent.asahi-u.ac.jp
authors: Mayumi
authors: M
authors: Iijima
authors: Chang
authors: C
authors: Du
authors: Christopher
authors: C
authors: Abbott
authors: Yutaka
authors: Y
authors: Doi
authors: Janet
authors: J
authors: Moradian-Oldak