title: Alternative pathway respiration and lipoxygenase activity in aged potato slice mitochondria.
abstract: Mitochondrial preparations isolated from aged white potato (Solanum tuberosum L.) slices exhibited classical cyanide-insensitive O(2) uptake which was inhibited by salicylhydroxamic acid and tetraethylthiuram disulfide (disulfiram). These mitochondria also possessed lipoxygenase activity, as determined by O(2) uptake in the presence of 4 millimolar linoleic acid. Purification of the mitochondrial preparation on a continuous Percoll gradient resulted in a large decrease in lipoxygenase activity whereas cyanide-insensitive (disulfiram sensitive) O(2) consumption was still observed. These data indicate that cyanide-insensitive O(2) consumption in mitochondrial preparations isolated from aged white potato slices is of mitochondrial origin and not due to lipoxygenase contamination.
authors: Department of Plant Science, University of Manitoba, Winnipeg, Canada R3T 2N2.
authors: R M
authors: RM
authors: Shingles
authors: G P
authors: GP
authors: Arron
authors: R D
authors: RD
authors: Hill