title: Inheritance of Resistance to Crown Gall in Pisum sativum.
abstract: We screened a total of 1365 pea (Pisum sativum) lines for response to inoculation with Agrobacterium tumefaciens, strain B6, and characterized resistance in one cultivar, Sweet Snap. Sweet Snap seedlings were highly resistant to tumorigenesis under most conditions. Resistance was overcome at inoculum concentrations of greater than 10(9) bacteria per milliliter. At such high concentrations, very small tumors developed on Sweet Snap in response to four wide-host-range Agrobacterium strains, but tumors on other cultivars were two-to sevenfold larger than those that formed on Sweet Snap. The hypervirulent strain A281 induced larger tumors on Sweet Snap than did other Agrobacterium strains, but tumors on other genotypes were more than 100% larger than those on Sweet Snap. Physiological experiments suggested that tumorigenesis in Sweet Snap is not blocked in early stages of infection, and genetic analysis indicated that inheritance of resistance to crown gall is a quantitative trait. In addition to the observed resistance in Sweet Snap, three ;supersusceptible' genotypes, which developed very large tumors, also were identified.
authors: Sugarcane Field Station, Canal Point, Florida 33438.
authors: S L
authors: SL
authors: Robbs
authors: M C
authors: MC
authors: Hawes
authors: H J
authors: HJ
authors: Lin
authors: S G
authors: SG
authors: Pueppke
authors: L Y
authors: LY
authors: Smith