title: Analysis of intragenic Ds transpositions and excision events generating novel allelic variation in rice.
abstract: Even though Ac/Ds gene-tagging systems have been established in many higher plants, maize is the only major plant in which short-distance transposition of Ac/Ds has been utilized to probe gene function. This study was performed to evaluate the efficiency of obtaining new alleles and functional revertants from Ds insertion loci in rice. By analyzing 1,580 plants and the progeny of selected lines, the insertion sites and orientations of Ds elements within 16 new heritable alleles of three rice loci were identified and characterized. Intragenic transposition was detected in both directions from the original insertion sites. The closest interval was 35 bp. Three of the alleles had two Ds elements in cis configuration in the same transcription units. We also analyzed the excision footprints of intragenic and extragenic transpositions in Ds-inserted alleles at 5 loci. The 134 footprints obtained from different plants revealed predominant patterns. Ds excision at each locus left a predominant footprint at frequencies of 30-75%. Overall, 66% of the footprints were 7-bp additions. In addition, 16% of the excisions left 0-, 3-, 6-, and 9-bp additions with the potential of conserving reading frame.
authors: Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors: Soon Ju
authors: SJ
authors: Park
authors: Hai Long
authors: HL
authors: Piao
authors: Yuan-hu
authors: YH
authors: Xuan
authors: Sung Han
authors: SH
authors: Park
authors: Byoung Il
authors: BI
authors: Je
authors: Chul Min
authors: CM
authors: Kim
authors: Eun Jin
authors: EJ
authors: Lee
authors: Soo Hyun
authors: SH
authors: Park
authors: ByeongChan
authors: B
authors: Ryu
authors: Kon Ho
authors: KH
authors: Lee
authors: Gi Hwan
authors: GH
authors: Lee
authors: Min Hee
authors: MH
authors: Nam
authors: Un Sang
authors: US
authors: Yeo
authors: Myung Chul
authors: MC
authors: Lee
authors: Doh Won
authors: DW
authors: Yun
authors: Moo Young
authors: MY
authors: Eun
authors: Chang-deok
authors: CD
authors: Han