title: A model for predicting ionic equilibrium concentrations in cell walls.
abstract: Purified cell walls were prepared from roots of Horse bean (Vicia faba L., var. minor) and Yellow Lupine (Lupinus luteus L.). Two methods were used: (a) grinding and (b) lysis of the endocellular contents by Triton X-100. The Ca(2+), Mg(2+), and K(+) contents were determined after incubation in various solutions in such a manner that the measurements gave access to the undisturbed equilibrium contents. The results were used to test a model which describes the ionic atmosphere in the cell walls as a result of simultaneous electrostatic interactions between free ions (Donnan effect) and specific association equilibria, including acido-basic ones. This model correctly accounted for the whole set of experimental results and predicted the values of the unmeasurable local concentrations and pH.
authors: Biochimie et Physiologie Végétale, ENSAM 34060 Montpellier Cedex, France.
authors: H
authors: H
authors: Sentenac
authors: C
authors: C
authors: Grignon