Identification of endogenous gibberellins from sorghum.
Gibberellins (GA) A(1), A(19), and A(20) were identified in shoot cylinders containing the apical meristems from sorghum (Sorghum bicolor L.). Extracts were purified by sequential SiO(2) partition chromatography and reversed-phase C(18) high performance liquid chromatography and biologically active (dwarf rice cv Tan-ginbozu microdrop assay) fractions were subjected to gas chromatography-selected ion monitoring. Based on the use of [(3)H]GA and [(2)H](d(2))GA internal standards, amounts of GA(1), GA(19), and GA(20) were estimated to be 0.7, 8.8, and 1.5 namograms per gram dry weight of tissue, respectively.
Rood
S B
SB
Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta T1K 3M4, Canada.
Larsen
K M
KM
Mander
L N
LN
Abe
H
H
Pharis
R P
RP