title: Enantioselective alpha-hydroxylation of 2-arylacetic acid derivatives and buspirone catalyzed by engineered cytochrome P450 BM-3.
abstract: Here we report that an engineered microbial cytochrome P450 BM-3 (CYP102A subfamily) efficiently catalyzes the alpha-hydroxylation of phenylacetic acid esters. This P450 BM-3 variant also produces the authentic human metabolite of buspirone, R-6-hydroxybuspirone, with 99.5% ee.
authors: Division of Chemistry and Chemical Engineering 210-41, California Institute of Technology, Pasadena, California 91125-4100, USA.
authors: Marco
authors: M
authors: Landwehr
authors: Lisa
authors: L
authors: Hochrein
authors: Christopher R
authors: CR
authors: Otey
authors: Alex
authors: A
authors: Kasrayan
authors: Jan-E
authors: JE
authors: Bäckvall
authors: Frances H
authors: FH
authors: Arnold