title: Plastocyanin as the possible site of photosynthetic electron transport inhibition by glutaraldehyde.
abstract: Treatment of spinach chloroplasts with glutaraldehyde causes an inhibition in the electron transport chain between the two photosystems. Measurements of O(2) flash yields, pH exchange, and fluorescence induction show that the O(2) evolving apparatus, photosystem II and its electron acceptor pool are not affected. The behavior of P700 indicates that its reduction but not its oxidation, is severely inhibited. Cytochrome f is still reducible by photosystem II but also slowly oxidizable by photosystem I. The sensitivity of isolated plastocyanin to glutaraldehyde further supports the conclusion that glutaraldehyde inhibits at the plastocyanin level and thereby induces a break between P700 and cytochrome f.
authors: Martin Marietta Laboratories, 1450 South Rolling Road, Baltimore, Maryland 21227.
authors: H
authors: H
authors: Hardt
authors: B
authors: B
authors: Kok