Hygromycin resistance gene cassettes for vector construction and selection of transformed rice protoplasts.
Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Pstl, and Sacll sites in the coding sequence of a hygromycin B phosphotransferase gene (hph) from Escherichia coli were eliminated. The mutated hph genes were used to form gene cassettes flanked by EcoRI-Sacll-Kpnl-Hindlll sites. Hygromycin resistance of wild-type and mutated hph genes was indistinguishable in E. coli and rice protoplast growth assay.
Zheng
Z
Z
Department of Plant Pathology and Crop Physiology, College of Agriculture, Louisiana State University, Baton Rouge, Louisiana 70803-1720.
Hayashimoto
A
A
Li
Z
Z
Murai
N
N