Novel approach to designing primers for identification and distinction of the human pathogenic fungi Coccidioides immitis and Coccidioides posadasii by PCR amplification.
We developed a pair of primers that specifically identifies Coccidioides species, etiologic agents of the human fungal disease coccidioidomycosis. These primers could be used for distinguishing Coccidioides immitis and Coccidioides posadasii by simply comparing the amplicon sizes on an agarose gel.
Umeyama
Takashi
T
Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
Sano
Ayako
A
Kamei
Katsuhiko
K
Niimi
Masakazu
M
Nishimura
Kazuko
K
Uehara
Yoshimasa
Y