title: Association of 70-kilodalton heat-shock cognate proteins with acclimation to cold.
abstract: Exposure of young spinach seedlings (Spinacia oleracea L. cv Bloomsdale) to 5 degrees C leads to an increase in the synthesis of several 79-kilodalton proteins that are present in leaf tissue grown at 20 degrees C. Protein sequence analyses and immunological cross-reactivity indicate that this group of proteins belongs to the 70-kilodalton heat-shock family. Steady-state transcript levels and protein synthesis are increased two- to threefold within 1 day, but immunoblot analyses suggest that the steady-state concentration of this protein group in leaf tissue only gradually accumulates at low temperature. It is proposed that the increased synthesis of several members of the 70-kilodalton heat-shock family could result from an influence of low temperature on protein folding and/or assembly processes.
authors: Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida 32611.
authors: L G
authors: LG
authors: Neven
authors: D W
authors: DW
authors: Haskell
authors: C L
authors: CL
authors: Guy
authors: N
authors: N
authors: Denslow
authors: P A
authors: PA
authors: Klein
authors: L G
authors: LG
authors: Green
authors: A
authors: A
authors: Silverman