title: Role of Pectinesterase in pH-Dependent Interactions between Pea Cell Wall Polymers.
abstract: Extracts of etiolated pea (Pisum sativum L.) shoots converted soluble pectin from the seedlings to a trichloroacetic acid-insoluble form. This activity coincided with pectinesterase peaks separated from the extracts by gel filtration and ion exchange. The conversion of pectin to the trichloroacetic acid-insoluble form and pectinesterase exhibited identical responses to pH, with activity only above pH 6. The formation of trichloroacetic acid-insoluble pectin in pea cell walls and their extracts is due to de-esterification of the pectin by pectinesterase and not to binding between pectin and a protein, as reported in the literature.
authors: Richard B. Russell Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, P. O. Box 5677, Athens, Georgia 30613.
authors: R
authors: R
authors: Pressey