Missense Mutation in the Chlamydomonas Chloroplast Gene that Encodes the Rubisco Large Subunit.
The 69-12Q mutant of Chlamydomonas reinhardtii lacks ribulose-1,5-bisphosphate carboxylase activity, but retains holoenzyme protein. It results from a mutation in the chloroplast large-subunit gene that causes an isoleucine-for-threonine substitution at amino-acid residue 173. Considering that lysine-175 is involved in catalysis, it appears that mutations cluster at the active site.
Spreitzer
R J
RJ
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, 68583-0718.
Brown
T
T
Chen
Z
Z
Zhang
D
D
Al-Abed
S R
SR