title: Association of latent cellulase activity with plasma membranes from kidney bean abscission zones.
abstract: Membranes isolated from abscission zones of Phaseolus vulgaris L., cv. Red Kidney, contained cellulase activity. This particulate activity was enhanced 10- to 20-fold by treatment with Triton X-100. Sucrose density gradient analyses of cell fractions showed that the membranes with which cellulase was associated had a peak equilibrium density of 1.16 to 1.17 g/cm(3) which coincided with that of ion-activated ATPase, a marker for plasma membranes. The membrane fraction having the highest cellulase activity also contained a high proportion of plasma membranes as shown by electron microscopy of sucrose density gradient fractions after staining by periodic acid-chromic acid-phosphotungstic acid. It was concluded that the particulate cellulase was associated with the plasma membrane.
authors: Departments of Plant Sciences and Biology, University of California, Riverside, California 92502.
authors: D E
authors: DE
authors: Koehler
authors: R T
authors: RT
authors: Leonard
authors: W J
authors: WJ
authors: Vanderwoude
authors: A E
authors: AE
authors: Linkins
authors: L N
authors: LN
authors: Lewis