Isoelectric focusing of cassava protoplasts.
Cassava (Manihot esculenta Crantz) protoplast was analyzed by using isoelectric focusing techniques. Two populations, representing 68 and 32% of the total sample, with mean isoelectric points of 4.48 and 4.60, were obtained using mesophyll protoplasts. The use of this technique allows demonstration of a discontinuous distribution of protoplast isoelectric point from one species according to their surface potential.
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Unidad de Biotecnología, Instituto Internacional de Estudios Avanzados, Apartado 17606, Caracas 1015A, Venezuela.
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