Plastid DNA content in a cultured soybean line capable of photoautotrophic growth.
The levels of chloroplast DNA in a cultured photoautotrophic soybean (Glycine max [L.] Merr. v Corsoy) cell line were determined by molecular hybridization. The cells were also grown photomixotrophically and heterotrophically as suspension cultures and the level of plastid DNA was found to be constant at approximately 26% of the total cellular DNA in all three growth modes. By comparison, total cellular DNA extracted from plants of the same variety used as the explant source for the cultured cells contained 12.3 to 18.9% (leaves and seeds) and 6.1 to 8.9% (roots) plastid DNA.
Cannon
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Department of Cell Biology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.
Heinhorst
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S
Weissbach
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A