title: Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target.
abstract: A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of detected peptides.
authors: Department of Electrical Measurements, Lund Institute of Technology, Lund University, Lund, Sweden.
authors: Simon
authors: S
authors: Ekström
authors: Lars
authors: L
authors: Wallman
authors: Daniel
authors: D
authors: Hök
authors: György
authors: G
authors: Marko-Varga
authors: Thomas
authors: T
authors: Laurell