An NADP-dependent Glutamate Dehydrogenase in Chloroplasts from the Marine Green Alga Caulerpa simpliciuscula.
NADP-dependent glutamate dehydrogenase was partially purified from extracts of the marine siphonous green alga Caulerpa simpliciuscula. The enzyme had an apparent Km NH(4) (+) of 0.4 to 0.7 mm and was highly specific for NADPH, alpha-ketoglutarate, and ammonium ions.The bulk of the NADP-glutamate dehydrogenase was isolated with the chloroplast fraction in cell-free preparations of this alga and was released from these "chloroplast fractions" as a soluble enzyme on gentle lysis of chloroplast membranes.
Gayler
K R
KR
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria 3052, Australia.
Morgan
W R
WR