title: Identification of genes preferentially expressed in articular cartilage by suppression subtractive hybridization.
abstract: Suppression subtractive hybridization is very effective to enrich differentially expressed genes in two different tissues or cells. We therefore used the technique to identify characteristic genes expressed in rat knee joint articular cartilage as compared to rat costal cartilage. In this study, we revealed that several genes were enriched in a subtracted articular cartilage cDNA library. The most enriched gene is lubricin that is a putative key molecule for joint lubrication. The second gene is milk fat globule epidermal growth factor (EGF) factor 8, MFG-E8 whose expression has never been observed in cartilage. Other enriched genes are known to be expressed in cartilage, however their differential expressions in cartilages have not been necessarily common. The preferential expression of characteristic genes in articular cartilage would provide unique properties to the tissue. Our findings will provide a new view of articular cartilage.
authors: Tissue Regeneration, Department of Hard Tissue Engineering, Tokyo Medical and Dental University 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors: Masayuki
authors: M
authors: Yoshimi
authors: Osamu
authors: O
authors: Miyaishi
authors: Sayaka
authors: S
authors: Nakamura
authors: Shin-Ichi
authors: S
authors: Shirasawa
authors: Hideaki
authors: H
authors: Kamochi
authors: Seiji
authors: S
authors: Miyatani
authors: Yoji
authors: Y
authors: Ikawa
authors: Tamayuki
authors: T
authors: Shinomura