title: Structural analysis of an extracellular polysaccharide produced by Rhodococcus rhodochrous strain S-2.
abstract: A possibility has been suggested of applying the EPS produced by Rhodococcus rhodochrous strain S-2 (S-2 EPS) to the bioremediation of oil-contaminated environments, because its addition, together with minerals, to oil-contaminated seawater resulted in emulsification of the oil, increased the degradation of polyaromatic hydrocarbons (PAH) of the oil, and led to the dominance of PAH-degrading marine bacteria. To understand the underlying principles of these phenomena, we determined the chemical structure of the sugar chain of S-2 EPS. The EPS was found to be composed of D-galactose, D-mannose, D-glucose, and D-glucuronic acid, in a molar ratio of 1:1:1:1. In addition, 0.8% (w/w) of octadecanoic acid and 2.7% (w/w) of hexadecanoic acid were also contained in its structure. By 1H and 13C NMR spectroscopy, including 2D DQF-COSY, TOCSY, HMQC, HMBC, and NOESY experiments, as well as chemical and enzymatic analyses, the polysaccharide was shown to consist of tetrasaccharide repeating units with the following structure: (see formula in text).
authors: Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
authors: Makoto
authors: M
authors: Urai
authors: Hirosi
authors: H
authors: Anzai
authors: Jun
authors: J
authors: Ogihara
authors: Noriyuki
authors: N
authors: Iwabuchi
authors: Shigeaki
authors: S
authors: Harayama
authors: Michio
authors: M
authors: Sunairi
authors: Mutsuyasu
authors: M
authors: Nakajima