title: Incorporation and Degradation of C and H-labeled Thymidine by Sugarcane Cells in Suspension Culture.
abstract: Sugarcane cells growing in suspension culture degrade exogenous thymidine, releasing thymine. Thymine is not utilized for DNA synthesis. Thymine is rapidly catabolized to beta-aminoisobutyric acid which is found within the cell. Thymidine in the medium is used for DNA synthesis. The label of [2-(14)C]thymidine is lost as (14)CO(2), but the label of [(3)H]methylthymidine is found in the cell as [(3)H]beta-aminoisobutyric acid, some of which is used for the synthesis of other cell components. The degradation of thymidine can be partially inhibited by addition of certain substituted pyrimidines.
authors: Chemistry and Biology Research Institute, Research Branch, Agriculture Canada, Ottawa K1A 0C6 Ontario.
authors: S M
authors: SM
authors: Lesley