title: Accumulation of Maltose during Photosynthesis in Protoplasts Isolated from Spinach Leaves Treated with Mannose.
abstract: When mannose was included in the enzyme incubation medium during the preparation of protoplasts from leaves of spinach, maltose was an early product of protoplast photosynthesis and, after 12 minutes, accounted for up to 15% of the (14)C incorporated from (14)CO(2). Maltose was not detected in protoplasts prepared in the normal enzyme medium. Rapid separation of cytoplasm and chloroplasts following exposure to (14)CO(2) showed that maltose was present in both fractions. Direct measurements of [(14)C]maltose uptake indicated transport across the chloroplast envelope at rates similar to the transport of glucose. The source of maltose and site of its initial formation are discussed.
authors: ARC Research Group on Photosynthesis, Department of Botany, University of Sheffield, S10 2TN, United Kingdom.
authors: A
authors: A
authors: Herold
authors: R C
authors: RC
authors: Leegood
authors: P H
authors: PH
authors: McNeil
authors: S P
authors: SP
authors: Robinson