Evidence for two ferryl species in chloroperoxidase compound II.
Using a combination of density functional calculations and Mössbauer spectroscopy, we have examined chloroperoxidase compound II (CPO-II). The Mössbauer spectrum of CPO-II suggests the presence of two distinct ferryl species in an approximately 70:30 ratio. Density functional calculations and cryogenic reduction and annealing experiments allow us to assign the major species as an Fe(IV)OH intermediate. The Mössbauer parameters of the minor component are indicative of an authentic iron(IV)oxo species, but we have found the 70:30 ratio to be pH invariant. The unchanging ratio of component concentrations is in agreement with CPO-II's visible absorption spectrum, which shows no change over the enzyme's range of pH stability.
Stone
Kari L
KL
Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Hoffart
Lee M
LM
Behan
Rachel K
RK
Krebs
Carsten
C
Green
Michael T
MT