Efficiency of particle-bombardment-mediated transformation is influenced by cell cycle stage in synchronized cultured cells of tobacco.
Plasmid DNA pB1221 harboring beta-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G(2) phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G(1) phases.
Iida
A
A
Sumitomo Chemical Co., Ltd., Biotechnology Laboratory, Takarazuka Research Center, 4-2-1 Takatsukasa, Takarazuka, Hyogo 665, Japan.
Yamashita
T
T
Yamada
Y
Y
Morikawa
H
H