title: Effect of CO(2) Concentration on Protein Biosynthesis and Carbonic Anhydrase Expression in Chlamydomonas reinhardtii.
abstract: The effect of external inorganic carbon (C(i)) concentrations on protein biosynthesis and carbonic anhydrase (CA) mRNA abundance were examined in the eukaryotic alga Chlamydomonas reinhardtii. Transfer of high CO(2) (5%) grown algae to air levels of CO(2) resulted in the transitory synthesis of two polypeptides of approximately 49,000 and 52,000 daltons as well as prolonged synthesis and accumulation of the 37,000 dalton CA monomer and an unidentified 20,000 dalton polypeptide. The gene coding for carbonic anhydrase was isolated from a genomic expression library and subjected to restriction endonuclease analysis. Southern blot analysis of chromosomal DNA indicates that only a single copy of the gene is present. The 2.5 kilobase DNA fragment hybridizes specifically to a 1.4 kilobase transcript in RNA isolated from air-grown cells and from cells grown on 5% CO(2) that have been exposed to air levels of CO(2). Maximum mRNA abundance was observed after 1 to 3 hours of exposure to air. Transfer of air-grown cells to a high CO(2) environment resulted in the elimination of the CA transcript after 60 minutes of exposure. Changes in CA transcript abundance in response to external C(i) concentrations occurred in the presence or absence of light.
authors: Centre for Plant Biotechnology, University of Toronto, Toronto, Ontario, Canada M5S 1A1.
authors: J
authors: J
authors: Bailly
authors: J R
authors: JR
authors: Coleman