Ultrasensitive DNA chip: gene expression profile analysis without RNA amplification.
We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis.
Nagino
Kunihisa
K
New Frontiers Research Laboratories, Toray Industries, Inc., Kanagawa 248-8555. Kunihisa_nagino@nts.toray.co.jp
Nomura
Osamu
O
Takii
Yuki
Y
Myomoto
Akira
A
Ichikawa
Makiko
M
Nakamura
Fumio
F
Higasa
Masashi
M
Akiyama
Hideo
H
Nobumasa
Hitoshi
H
Shiojima
Satoshi
S
Tsujimoto
Gozoh
G