Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma.
Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10,000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.
Gaspari
Marco
M
Department of Clinical and Experimental Medicine, Magna Graecia University, 88100 Catanzaro, Italy. Gaspari@unicz.it
Ming-Cheng Cheng
Mark
M
Terracciano
Rosa
R
Liu
Xuewu
X
Nijdam
A Jasper
AJ
Vaccari
Lisa
L
di Fabrizio
Enzo
E
Petricoin
Emanuel F
EF
Liotta
Lance A
LA
Cuda
Giovanni
G
Venuta
Salvatore
S
Ferrari
Mauro
M