Detection of the messenger RNA encoding for the ferredoxin-dependent glutamate synthase in maize leaf.
Ferredoxin-dependent glutamate synthase (EC 1.4.7.1), glutamate oxoglutarate aminotransferase (glutamate synthase) (GOGAT) messenger RNA was extracted from maize (Zea mays L.) leaves and partially purified through oligo(dT)-cellulose chromatography and ultracentrifugation in a sucrose gradient. mRNA were translated in vitro using a reticulocyte system. The glutamate synthase subunit was characterized by immunoprecipitation with antibodies raised against the rice (Oryza sativa L.) ferredoxin-glutamate synthase. The in vitro synthesized protein and the 145 kilodaltons genuine maize leaf subunit of GOGAT were found to comigrate in sodium dodecyl sulfate-polyacrylamide gel electrophoresis experiments.
Commere
B
B
Laboratoire de Biologie Cellulaire, INRA CNRA, Route de St Cyr, 78000 Versailles, France.
Vidal
J
J
Suzuki
A
A
Gadal
P
P
Caboche
M
M