title: High resolution spectral differentiation of enantiomers: benzo[a]pyrene tetrols complexed with a promiscuous antibody.
abstract: The interaction of the highly cross-reactive anti-PAH monoclonal antibody with four diastereomeric benzo[a]pyrene tetrols (BPTs) is studied by means of fluorescence line-narrowing spectroscopy. It is shown that the interaction of enantiomers of cis-BPT and trans-BPT with the antibody involves different complex geometries. These spatially different ligand-protein interactions alter the relative intensities of the excited-state vibrational frequencies of immunocomplexed molecules allowing for unambiguous spectroscopic resolution of all four enantiomeric isomers. This study represents the first example of a high-resolution, fluorescence-based spectroscopic method capable of enantiospecific differentiation.
authors: Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
authors: Nenad M
authors: NM
authors: Grubor
authors: Ying
authors: Y
authors: Liu
authors: Xinxin
authors: X
authors: Han
authors: Daniel W
authors: DW
authors: Armstrong
authors: Ryszard
authors: R
authors: Jankowiak