title: Suberin production by isolated tomato fruit protoplasts.
abstract: The multilamellar wall secreted by protoplasts isolated from locule tissue of tomato (Lycopersicon esculentum L.) fruit was purified, and an extract was obtained after depolymerization with BF(3)-methanol. Analysis of this extract using thin layer chromatography demonstrated the presence of fatty acid methyl esters, fatty alcohols, dicarboxylic acid dimethyl esters, and omega-hydroxy acid methyl esters. These components were quantified using an Iatroscan thin layer chromatography-flame ionization detection system. The different chain lengths in each group were identified and quantified using gas chromatography. The results clearly indicated the presence of suberin.
authors: Biology Department, Dalhousie University, Halifax, Nova Scotia B3H 4J1 Canada.
authors: G S
authors: GS
authors: Rao
authors: J H
authors: JH
authors: Willison
authors: W M
authors: WM
authors: Ratnayake