[Isolation and identification of hetisine-type alkaloids from Aconitum coreanum by high speed countercurrent chromatography].
OBJECTIVE
To search for more bioactive compounds from the roots of Aconitum coreanum (Lèvl.) Rapaics.


METHODS
High speed countercurrent chromatography was successfully applied to the separation of alkaloids from Aconitum coreanum. The structures were elucidated by their physicochemical properties and spectroscopic analysis.


RESULTS
Two-phase solvent system composed of CHCl3-CH3OH-0.2 mol x L(-1) HCl (10:3:3, volume ratio) was used in this experiment, eight alkaloids were obtained from the roots of Aconitum coreanum, which were identified as: 2alpha-propionyl-11alpha,13beta-diacetyl-14-hydroxyhetisine (I), Guanfu base P (II), Guanfu base G (III), Guanfu base F (IV), Guanfu base Z (V), Guanfu base O (VI), Guanfu base A (VII), Guanfu base B (VIII).


CONCLUSIONS
Compound I is a new alkaloid, named Guanfu base R.
Jiang
Kai
K
Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009, China.
Yang
Chun-hua
CH
Liu
Jing-han
JH
Tang
Qing-fa
QF