[Mutations of DNA gyrase and topoisomerase IV in clinical isolates of fluoroquinolone-resistant Proteus mirabilis].
The presence of fluoroquinolone resistance-associated mutations within the quinolone resistance-determining region of DNA gyrase and topoisomerase IV was investigated genetically in clinical isolates of Proteus mirabilis recovered from patients with urinay tract infections. Two isolates of fluoroquinolone-resistant P. mirabilis possessed the mutations in GyrA (Ser-83 --> Arg or Ile), GyrB (Ser-464 --> Tyr or Phe) and ParC (Ser-80 --> Ile). A novel mutation with Glu-87 --> Lys in GyrA, where suggested to be responsible for fluoroquinolone resistance, was identified. These results demonstrate that the presence of an additional mutation at Glu-87 in GyrA may contribute to high-level fluoroquinolone resistance, too.
Saito
Ryoichi
R
Department of Infection Control and Prevention, The University of Tokyo Hospital, Bunkyo-ku, Tokyo 113-8655, Japan.
Sato
Kenya
K
Kumita
Wakako
W
Inami
Natsuko
N
Nishiyama
Hiroyuki
H
Okamura
Noboru
N
Moriya
Kyoji
K
Koike
Kazuhiko
K