Electron Spin Resonance of 2,2,6,6-Tetramethylpiperidine-1-Oxyl (TEMPO)-labeled Plant Leaves.
Temperature dependence for partitioning of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) between aqueous and lipid components of whole leaf tissue was measured. TEMPO is an electron spin resonance nitroxide label that has been used in model systems to detect membrane phase separations. Measurements were made on chilling-sensitive tomato leaves, frost-sensitive potato leaves, and frost-hardy and supercooled wheat leaves. The results suggest a membrane phase separation at 11 C in tomato, 3 C in potato, and -11 C in wheat.
Fey
R L
RL
Department of Horticulture, Colorado State University, Fort Collins, Colorado 80523.
Workman
M
M
Marcellos
H
H
Burke
M J
MJ