title: Naturally occurring biflavonoid, ochnaflavone, inhibits cyclooxygenases-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells.
abstract: Ochnaflavone is a medicinal herbal product isolated from Lonicera japonica that inhibits cyclooxygenase-2 (COX-2) dependent phases of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner with IC50 values of 0.6 microM. Western blotting probed with specific anti-COX-2 antibodies showed that the decrease in quantity of the PGD2 product was accompanied by a decrease in the COX-2 protein level. In addition, this compound consistently inhibited the production of leukotriene C4 (LTC4) in a dose dependent manner, with an IC50 value of 6.56 microM. These results demonstrate that ochnaflavone has a dual cyclooxygenase-2/5-lipoxygenase inhibitory activity. Furthermore, this compound strongly inhibited degranulation reaction in a dose dependent manner, with an IC50 value of 3.01 microM. Therefore, this compound might provide a basis for novel anti-inflammatory drugs.
authors: Skeletal Diseases Genome Research Center, Kyungpook National University, Daegu 702-701, Korea.
authors: Min Jung
authors: MJ
authors: Son
authors: Tae Chul
authors: TC
authors: Moon
authors: Eun Kyung
authors: EK
authors: Lee
authors: Kun Ho
authors: KH
authors: Son
authors: Hyun Pyo
authors: HP
authors: Kim
authors: Sam Sik
authors: SS
authors: Kang
authors: Jong Keun
authors: JK
authors: Son
authors: Seung Ho
authors: SH
authors: Lee
authors: Hyeun Wook
authors: HW
authors: Chang