title: Screening for mutations affecting sexual reproduction after activation tagging in Arabidopsis thaliana.
abstract: In this work, a seed-set-based screening was performed on 70 lines of Arabidopsis thaliana after activation tagging mutagenesis to identify mutations in reproductive mechanisms. Five mutants showed significantly lower seed set than the wild type and confirmed the phenotype in the progeny. This phenotype was linked with the marker gene bar carried by T-DNA conferring glufosinate resistance. Genetic analysis revealed that the mutation inheritance was sporophytic in 3 mutants and gametophytic in 2 mutants. In addition, 2 mutants had an extra T-DNA copy. Thus activation tagging can be an effective strategy to identify new mutations affecting sporogenesis or gametogenesis.
authors: DISSPA, Dept. of Soil, Plant and Environmental Sciences, University of Naples Federico II, Portici, Italy.
authors: Giorgio
authors: G
authors: Perrella
authors: Gaetana
authors: G
authors: Cremona
authors: Federica
authors: F
authors: Consiglio
authors: Angela
authors: A
authors: Errico
authors: Ray A
authors: RA
authors: Bressan
authors: Clara
authors: C
authors: Conicella