Metabolism of Glutamate in Suspension Cultures of Paul's Scarlet Rose Cells.
When specifically labeled glutamate-1-(14)C was provided to 4-day-old rose cells, 87.6% of the (14)C in glutamate recovered from protein was in the number 1 carbon atom of the glutamate molecule. It was concluded that newly absorbed glutamate was incorporated directly into protein without any prior metabolism.A double labeling study with acetate-U-(14)C and glutamate-U-(3)H showed the availability of absorbed glutamate for protein synthesis was equal to that of endogenously synthesized glutamate.
Fletcher
J S
JS
Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73069.