Preprocessing of tandem mass spectrometric data based on decision tree classification.
In this study, we present a preprocessing method for quadrupole time-of-flight (Q-TOF) tandem mass spectra to increase the accuracy of database searching for peptide (protein) identification. Based on the natural isotopic information inherent in tandem mass spectra, we construct a decision tree after feature selection to classify the noise and ion peaks in tandem spectra. Furthermore, we recognize overlapping peaks to find the monoisotopic masses of ions for the following identification process. The experimental results show that this preprocessing method increases the search speed and the reliability of peptide identification.
Zhang
Jing Fen
JF
Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China, China. jfzhang@jdl.ac.cn
He
Si Min
SM
Cai
Jin Jin
JJ
Cao
Xing Jun
XJ
Sun
Rui Xiang
RX
Fu
Yan
Y
Zeng
Rong
R
Gao
Wen
W