title: Influence of culture age and spermidine treatment on the accumulation of phenolic compounds in suspension cultures.
abstract: The influence of cell age on phenol accumulation was examined by determining the quantity of individual phenols which accumulated in Paul's scarlet rose cultures of increasing age. During log-phase growth (days 7 and 11), only gallic acid and epicatechin-catechin were detected; whereas, during early and late stationary phase (days 14 and 35), several other phenols were present in addition to gallic acid and epicatechincatechin. When stationary-phase cultures were provided with a supplement of sucrose and spermidine, a treatment previously shown to arrest the senescence of rose cultures (Muhitch, Edwards, Fletcher 1983 Plant Cell Rep 2: 82-84), the cells then accumulated a higher level and a wider assortment of phenols. These results suggest that extending the lifespan of mature nondividing cell cultures offers a means of increasing the yield of secondary products by cultured cells.
authors: Botany and Microbiology Department, University of Oklahoma, Norman, Oklahoma 73019.
authors: M J
authors: MJ
authors: Muhitch
authors: J S
authors: JS
authors: Fletcher