[Optimizing culture system of mouse marrow mesenchymal stem cells in vitro and investigating effect of alcohol and acetaldehyde on the cells].
OBJECTIVE
To optimize the in vitro culture system of C57/BL6 marrow mesenchymal stem cells (MSCs) and to investigate the effect of alcohol and acetaldehyde on MSCs.


METHODS
The MSCs were isolated from the femur marrow of C57/BL6 mice and were cultured in the optimized system, so that highly-purified MSCs were harvested and identified by immunohistochemistry. Then, MSCs were cultured in the medium containing alcohol or its metabolic product acetaldehyde, with the following concentration groups: alcohol 5.7, 17.0, 50.0, 100.0 and 150.0 mmol/L; acetaldehyde 4.5, 0.9, 0.18, 0.036, 0.0072, 0.00144, 0.000288 mmol/L. MSCs were cultured with alpha-MEM as the control group. After 3 days, their proliferation activity was measured by the MTT method.


RESULTS
MSCs within 6 passages had a good stability and a high proliferation activity. They were identified to express CD90 but no CD34. The MTT assay showed that alcohol at the concentration greater than 100.0 mmol/L and acetaldehyde at the concentration greater than 4.5 mmol/L could inhibit proliferation of MSCs (P < 0.05). But the proliferation activity might rise with an increase in the acetaldehyde concentration smaller than 0.18 mmol/L (P < 0.05).


CONCLUSIONS
The optimized culture system can effectively isolate and culture MSCs. Both alcohol and acetaldehyde can inhibit proliferation of MSCs but toxicity of acetaldehyde is more serious.
Li
Hong
H
Deparment of Environmental Health, College of Public Health, Sichuan University, Chengdu Sichuan, 610041, PR China.
Qu
Weidong
W
Wu
Desheng
D