title: Separation of Mg-Protoporphyrin IX and Mg-Protoporphyrin IX Monomethyl Ester Synthesized de novo by Developing Cucumber Etioplasts.
abstract: High pressure liquid chromatography was used to demonstrate that chelation of Mg(2+) into protoporphyrin IX precedes methylation in isolated greening etioplasts from cucumber (Cucumis sativus L. var. Beit Alpha) cotyledons. Mg-protoporphyrin IX synthesized in vitro from protoporphyrin IX, Mg(2+), and ATP or exogenous Mg-protoporphyrin IX could serve as substrates for the methylation step. In either case, S-adenosylmethionine was the methyl donor and could not be replaced by ATP plus methionine.
authors: Department of Botany, University of California, Davis, California 95616.
authors: T P
authors: TP
authors: Fuesler
authors: C M
authors: CM
authors: Hanamoto
authors: P A
authors: PA
authors: Castelfranco