title: Effect of azetidine 2-carboxylic Acid on ion uptake and ion release to the xylem of excised barley roots.
abstract: Azetidine 2-carboxylic acid (AZ) was used as an analog of proline to investigate further the relationship between protein synthesis and ion transport. AZ does not inhibit protein assembly, but the proteins formed are ineffective as enzymes. At relatively low concentrations (50 muM) AZ was a potent inhibitor of release of ions to the xylem of excised roots of barley (Hordeum vulgare L.) and intact plants. Uptake to the root was also inhibited but to a lesser degree. A procedure was introduced for estimating unidirectional fluxes from measurements of net tracer uptake, net transport to the xylem, and net efflux from the roots. It was shown that inhibition of release to the xylem was not caused by reduction in influx at the plasmalemma or to stimulation of influx to the vacuoles. It was suggested that AZ was acting on the process of release from symplast to the xylem. The action of AZ is compared with similar effects on ion transport produced by p-fluorophenylalanine, cycloheximide, and abscisic acid.
authors: School of Biological Sciences, University of Sydney, N.S.W., Australia 2006.
authors: M G
authors: MG
authors: Pitman
authors: R A
authors: RA
authors: Wildes
authors: N
authors: N
authors: Schaefer
authors: D
authors: D
authors: Wellfare