title: Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist Clenbuterol residues.
abstract: A rapid immunochromatographic lateral flow test strip was developed in a competitive format with the gold-conjugated monoclonal antibody to specifically determine the residues of Clenbuterol (CL), a beta-adrenergic agonist. The test strip is made up of a sample pad, a conjugate reagent pad, a test membrane containing a control line and a test line, and an absorbent pad. CL standard samples of 0, 0.1, 0.3, 0.9, 2.7, 8.1 ng/ml in swine urine were determined by the test strip. It was shown that detection limit of the test strip was as low as 0.1 ng/ml of CL and that the half of maximal inhibition concentration (IC50) in relative optical density was calculated to be 1.78+/-0.17 ng/ml under an optical density scanner. The sensitivity by eye measurement was 1.0 ng/ml. It takes 10 min to accomplish a test. Parallel analysis of urine samples from pigs fed with CL showed comparable results obtained from the test strip and GC-MS. Therefore, the test strip is very useful as a screening method for quantitative, semi-quantitative or qualitative detection of CL residues in swine urine.
authors: College of Animal and Veterinary Sciences, Henan Institute of Science and Technology, Xinxiang 453003, PR China. zhanggaiping2003@yahoo.com.cn
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authors: GP
authors: Zhang
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authors: Wang
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authors: Yang
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authors: Yang
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authors: Xing
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authors: Li
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authors: Zhao
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authors: Chai
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