Intrasplenic steady-state dendritic cell precursors that are distinct from monocytes.
Immediate precursors of the many subtypes of dendritic cells (DCs) remain obscure. Here we purified a splenic precursor population that produced all splenic CD8+ and CD8- conventional DCs (cDCs) but not plasmacytoid DCs or other lineages. This 'pre-cDC' population included cells 'precommitted' to form either CD8+ or CD8- cDCs. The pre-cDCs, which comprised 0.05% of splenocytes, expressed a CD11c(int) CD45RA(lo) CD43(int) SIRP-alpha(int) CD4- CD8- major histocompatibility complex class II-negative surface phenotype. The pre-cDCs were not monocytes. Monocytes generated few cDCs in steady-state recipient mice. However, when transferred into mice with an inflammatory milieu dependent on granulocyte-macrophage colony-stimulating factor, monocytes produced a distinct type of splenic DC. Thus, the inflammatory status of the host influences the developmental origin and type of DC present in lymphoid tissues.
Naik
Shalin H
SH
The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Vaccine Technology, Parkville, Victoria 3050, Australia. naik@wehi.edu.au
Metcalf
Donald
D
van Nieuwenhuijze
Annemarie
A
Wicks
Ian
I
Wu
Li
L
O'Keeffe
Meredith
M
Shortman
Ken
K