title: UV embossed polymeric chip for protein separation and identification based on capillary isoelectric focusing and MALDI-TOF-MS.
abstract: This paper demonstrates a ultraviolet (UV)-embossed polymeric chip for protein separation by capillary isoelectric focusing (CIEF) and identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The polymeric chip was replicated by a UV-embossing technique using a soft rubber mold. Five diverse widely investigated families of UV-cured formulations were examined for MALDI ionization efficiency of bovine serum albumin (BSA) samples spotted on the polymer surfaces. The signal and signal-to-noise ratio from the polyester formulation were each 12 times those obtained with PMMA (a commonly used polymer material for MALDI) at picomole sample concentration. A polyester chip was successfully used to carry out CIEF to separate proteins, followed by MALDI-TOF-MS identification. Issues related to the successful chip fabrication and protein separation and identification are discussed.
authors: Singapore-MIT Alliance, N2-B2c-15, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.
authors: Xun
authors: X
authors: Guo
authors: Mary B
authors: MB
authors: Chan-Park
authors: Soon Fatt
authors: SF
authors: Yoon
authors: Jung-hoon
authors: JH
authors: Chun
authors: Lin
authors: L
authors: Hua
authors: Newman S-K
authors: NS
authors: Sze