Evaluations of substrate specificity and inhibition at PR/p3 cleavage site of HTLV-1 protease.
Core sequences necessary for substrate recognition and its inhibition at the PR/p3 site of HTLV-1 protease were clarified for the first time. From the cleavage rates of peptides containing a part of the PR/p3 site, a heptapeptide was found to be the minimal sequence required for substrate recognition. The use of synthetic inhibitors containing hydroxyethylamine dipeptide isostere indicated that a tetrapeptide sequence was necessary to achieve potent inhibition.
Naka
Hiromi
H
Institute for Protein Research, Osaka University, Osaka 565-0871, Japan.
Teruya
Kenta
K
Bang
Jeong Kyu
JK
Aimoto
Saburo
S
Tatsumi
Tadashi
T
Konno
Hiroyuki
H
Nosaka
Kazuto
K
Akaji
Kenichi
K