title: The effects of alpha-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimurium and Shigella flexneri.
abstract: Two milk components, alpha-lactalbumin (alpha-La) and glycomacropeptide (GMP) may inhibit intestinal infection/intoxification. (3)[H] thymidine-labeled enteropathogenic Escherichia coli (EPEC), Salmonella typhimurium (ATCC 6994) or Shigella flexneri (ATCC 9199) were introduced to CaCo-2 cultures and their association with CaCo-2 cells was assessed. Undigested, pepsin-digested and pepsin- and pancreatin-digested alpha-lactalbumin and glycomacropeptide inhibited association. Thus, milk supplemented with alpha-lactalbumin and glycomacropeptide might be effective in inhibiting associations of the pathogens EPEC, Salmonella typhimurium, and Shigella flexneri to intestinal cells.
authors: Food Microbial Sciences Unit, School of Food Biosciences, The University of Reading, Reading, UK. w_bruck@hotmail.com
authors: Wolfram M
authors: WM
authors: Brück
authors: Shannon L
authors: SL
authors: Kelleher
authors: Glenn R
authors: GR
authors: Gibson
authors: Gitte
authors: G
authors: Graverholt
authors: Bo L
authors: BL
authors: Lönnerdal