Transient expression of chimeric genes delivered into pollen by microprojectile bombardment.
Chimeric genes containing a pollen-specific promoter from tomato (Lycopersicon esculentum) or the CaMV35S promoter were transiently expressed following their introduction into tobacco (Nicotiana tabacum) pollen using high velocity microprojectiles. Transient expression of the microprojectile-introduced genes in leaves and pollen was similar to that observed for these genes in stably transformed tobacco plants.
Twell
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U.S. Department of Agriculture-Agricultural Research Service Plant Gene Expression Center, Albany, California 94710.
Klein
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Fromm
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McCormick
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