Techniques in gerontology: cell lines as standards for telomere length and telomerase activity assessment.
The length of telomeres is believed to critically influence cellular aging processes and disease development. In order to reliably monitor telomere length and the corresponding cellular telomerase activity by optimized procedures, either based on flow cytometry or quantitative PCR technique, we here propose three commonly used cell lines, HEK293, K562 and TCL1301 as standards. In this contribution, efficient methods to determine mean telomere length of eukaryotic chromosomal DNA and determination of the corresponding telomeras activity are outlined. In particular, wide-range standard curves for a precise assessment of telomere length of genomic DNA by quantitative PCR technique are presented, measures, which greatly simplify the evaluation of respective functional roles of telomeres when studying biological processes such as disease progression and aging.
Fehrer
Christine
C
Institute for Biomedical Aging Research, Austrian Academy of Sciences, Rennweg 10, A-6020 Innsbruck, Austria.
Voglauer
Regina
R
Wieser
Matthias
M
Pfister
Gerald
G
Brunauer
Regina
R
Cioca
Daniel
D
Grubeck-Loebenstein
Beatrix
B
Lepperdinger
Günter
G