title: Superoxide free radicals are produced in glyoxysomes.
abstract: The production of superoxide free radicals in pellet and supernatant fractions of glyoxysomes, specialized plant peroxisomes from watermelon (Citrullus vulgaris Schrad.) cotyledons, was investigated. Upon inhibition of the endogenous superoxide dismutase, xanthine, and hypoxanthine induced in glyoxysomal supernatants the generation of O(2) (-) radicals and this was inhibited by allopurinol. In glyoxysomal pellets, NADH stimulated the generation of superoxide radicals. Superoxide production by purines was due to xanthine oxidase, which was found predominantly in the matrix of glyoxysomes. The generation of O(2) (-) radicals in glyoxysomes by endogenous metabolites suggests new active oxygen-related roles for glyoxysomes, and for peroxisomes in general, in cellular metabolism.
authors: Unidad de Bioquímica Vegetal, Estación Experimental del Zaidín, C.S.I.C., Aptdo. 419, 18080 Granada, Spain.
authors: L M
authors: LM
authors: Sandalio
authors: V M
authors: VM
authors: Fernández
authors: F L
authors: FL
authors: Rupérez
authors: L A
authors: LA
authors: Del Río