Detection of Norspermidine and Norspermine in Medicago sativa L. (Alfalfa).
Shoot meristem tissues of alfalfa, Medicago sativa L., were found by high performance liquid chromatography analyses to contain the uncommon polyamines, norspermidine and norspermine. The chemical structures of norspermidine and norspermine, purified from alfalfa, were confirmed by comparison of mass spectra with those from authentic standards. The discovery of norspermidine and norspermine in alfalfa implicates the presence of at least two biosynthetic enzymes, a polyamine oxidase and a previously uncharacterized aminopropyltransferase.
Rodriguez-Garay
B
B
Department of Agronomy and Horticulture, Plant Genetic Engineering Laboratory, New Mexico State University, Las Cruces, New Mexico 88003.
Phillips
G C
GC
Kuehn
G D
GD