title: Temperature Effects on Phosphoenolpyruvate Carboxylase from a CAM and a C(4) Plant : A Comparative Study.
abstract: The effect of temperature in the range from 10 to 35 degrees C on various characteristics of phosphoenolpyruvate carboxylase from the leaves of a CAM plant, Crassula argentea and a C(4) plant Zea mays shows a number of different effects related to the environment in which these distinct types of metabolic specialization normally operate. The Arrhenius plot of V(max) for the two enzyme forms shows that the CAM enzyme has a linear increase with temperature while the C(4) enzyme has an inflection at 27 degrees C implying a conformational or aggregational change in the enzyme or a shift in reaction mechanism to one requiring a lower activation energy. The Arrhenius plot of K(m) for the two enzymes reveals the startling fact that at temperatures above 20 degrees C an increasing temperature causes an increase in K(mPEP) for the CAM enzyme while the C(4) enzyme displays a decreased K(m) as the temperature increases. The inhibitory effect of 5 millimolar malate also shows opposite trends for the two enzymes. For the CAM enzyme the percent inhibition by malate increases from essentially none at 15 degrees C to 70% at 35 degrees C. For the C(4) enzyme the percent inhibition drops from about 60% at 20 degrees C to 2% at 30 degrees C. Similar opposite behavior of the two enzymes is found with the K(i) for malate. Pretreatment at high temperatures for periods up to 2 hours was found to result in differences similar to those described above if the treated enzyme were subsequently assayed at 25 degrees C.
authors: Department of Biochemistry, University of California, Riverside, California 92521.
authors: M X
authors: MX
authors: Wu
authors: R T
authors: RT
authors: Wedding