title: Effect of paclobutrazol on water stress-induced abscisic Acid in apple seedling leaves.
abstract: Abscisic acid (ABA) was quantitated by enzyme-linked immunosorbent assay (ELISA) in water-stressed leaves from control apple seedlings, and also from apple seedlings treated for 28 days with paclobutrazol ([2RS, 3RS]-1-[4-chlorophenyl]-4,4-dimethyl-2-[1,2,4-triazol-1-yl] pentan-3-ol). The ELISA quantitative estimates were also validated by gas chromatography-electron capture detector and lettuce seed germination inhibition bioassay. Paclobutrazol treatment reduced endogenous ABA levels by about one-third, and prevented the marked accumulation of water-stress-induced ABA that occurred in untreated seedlings. The presence of ABA in the apple leaf extracts was confirmed by gas chromatography-mass spectrometry.
authors: Fruit Laboratory, Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705.
authors: S Y
authors: SY
authors: Wang
authors: T
authors: T
authors: Sun
authors: Z L
authors: ZL
authors: Ji
authors: M
authors: M
authors: Faust