title: Effect of salivary stimulation on erosion of human and bovine enamel subjected or not to subsequent abrasion: an in situ/ex vivo study.
abstract: This in situ/ex vivo study evaluated whether saliva stimulated by chewing gum could prevent or reduce the wear and the percent change in microhardness (%SMH) of bovine and human enamel submitted to erosion followed by brushing abrasion immediately or after 1 h. During 2 experimental 7-day crossover phases, 9 previously selected volunteers wore intraoral palatal devices, with 12 enamel specimens (6 human and 6 bovine). In the first phase, the volunteers immersed the device for 5 min in 150 ml of cola drink, 4 times per day (at 8, 12, 16 and 20 h). Immediately after the immersions, no treatment was performed in 4 specimens, 4 other specimens were immediately brushed (0 min) using a fluoride dentifrice, and the device was replaced into the mouth. After 60 min, the remaining 4 specimens were brushed. In the second phase, the procedures were repeated, but after the immersions, the volunteers stimulated the salivary flow rate by chewing a sugar-free gum for 30 min. Changes in wear and %SMH were measured. ANOVA and Tukey's test showed statistical differences (p<0.05) for the following comparisons. The chewing gum promoted less wear and %SMH. A decreasing %SMH and an increasing enamel wear were observed in the following conditions: erosion only, 60 min and 0 min. The human enamel presented greater %SMH and less wear compared to bovine enamel. The data suggest that the salivary stimulation after an erosive or erosive/abrasive attack can reduce the dental wear and the %SMH.
authors.affiliation: Department of Pediatric Dentistry, Orthodontics and Public Health, Faculty of Dentistry of Bauru, University of São Paulo, Bauru, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Rios
authors.forename: H M
authors.initials: HM
authors.lastname: Honório
authors.forename: A C
authors.initials: AC
authors.lastname: Magalhães
authors.forename: A C B
authors.initials: AC
authors.lastname: Delbem
authors.forename: M A A M
authors.initials: MA
authors.lastname: Machado
authors.forename: S M B
authors.initials: SM
authors.lastname: Silva
authors.forename: M A R
authors.initials: MA
authors.lastname: Buzalaf
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000818:Animals; D002253:Carbonated Beverages / Q000009:adverse effects*; D002417:Cattle; D018592:Cross-Over Studies; D005260:Female; D006244:Hardness; D006801:Humans; D008297:Male; D012463:Saliva / Q000378:metabolism*; D013997:Time Factors; D014072:Tooth Abrasion / Q000517:prevention & control*; D014077:Tooth Erosion / Q000517:prevention & control*; D014099:Toothbrushing / Q000009:adverse effects*