title: Antioxidative and acute antiinflammatory effects of Torreya grandis.
abstract: The present study was undertaken to investigate the antioxidative and antiinflammatory activities of the ethanolic extract of seeds of Torreya grandis (EST). Exposure of human dermal fibroblasts to the extract at 50 and 250 microg/ml showed significant protective effect against hydrogen peroxide (300 microM). EST not only protected cell survival from H(2)O(2)-induced toxicity, but also inhibited the H(2)O(2)-induced LDH release significantly. It was also found that EST at 100 and 1000 microg/ml showed scavenging activities of radicals and reactive oxygen species with 29.8% and 100.0% of inhibition against DPPH radical and 41.2% and 98.4% against superoxide radicals in the xanthine/xanthine oxidase system, respectively. Topically applied EST dose-dependently inhibited arachidonic acid (AA)- and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice.
authors.affiliation: Department of Pharmacology, Peking University, School of Basic Medical Science, 38 Xueyuan Lu, Beijing 100083, China.
authors.forename: Bao-Qiong
authors.initials: BQ
authors.lastname: Chen
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Cui
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Yong-He
authors.initials: YH
authors.lastname: Zhang
authors.forename: Hui-Shan
authors.initials: HS
authors.lastname: Piao
authors.forename: Jin-Hwa
authors.initials: JH
authors.lastname: Kim
authors.forename: Bum-Chun
authors.initials: BC
authors.lastname: Lee
authors.forename: Hyeong-Bae
authors.initials: HB
authors.lastname: Pyo
authors.forename: Yeo-Pyo
authors.initials: YP
authors.lastname: Yun
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D016718:Arachidonic Acid; D001713:Biphenyl Compounds; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control*; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D006861:Hydrogen Peroxide; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012639:Seeds; D013481:Superoxides / Q000737:chemistry; D032490:Taxaceae; D013755:Tetradecanoylphorbol Acetate
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D013481:Superoxides; D016718:Arachidonic Acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D013755:Tetradecanoylphorbol Acetate