title: Comparative genomic analysis of two strains of human adenovirus type 3 isolated from children with acute respiratory infection in southern China.
abstract: Human adenovirus type 3 (HAdV-3) is a causative agent of acute respiratory disease, which is prevalent throughout the world, especially in Asia. Here, the complete genome sequences of two field strains of HAdV-3 (strains GZ1 and GZ2) isolated from children with acute respiratory infection in southern China are reported (GenBank accession nos DQ099432 and DQ105654, respectively). The genomes were 35,273 bp (GZ1) and 35,269 bp (GZ2) and both had a G+C content of 51 mol%. They shared 99% nucleotide identity and the four early and five late regions that are characteristic of human adenoviruses. Thirty-nine protein- and two RNA-coding sequences were identified in the genome sequences of both strains. Protein pX had a predicted molecular mass of 8.3 kDa in strain GZ1; this was lower (7.6 kDa) in strain GZ2. Both strains contained 10 short inverted repeats, in addition to their inverted terminal repeats (111 bp). Comparative whole-genome analysis revealed 93 mismatches and four insertions/deletions between the two strains. Strain GZ1 infection produced a typical cytopathic effect, whereas strain GZ2 did not; non-synonymous substitutions in proteins of GZ2 may be responsible for this difference.
authors.affiliation: Central Laboratory, Guangzhou Children's Hospital, Guangzhou 510120, China.|State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: South China Sea Institute of Oceanology, LED, Chinese Academy of Sciences, Guangzhou 510301, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Su
authors.affiliation: Central Laboratory, Guangzhou Children's Hospital, Guangzhou 510120, China.
authors.forename: Sitang
authors.initials: S
authors.lastname: Gong
authors.affiliation: Central Laboratory, Guangzhou Children's Hospital, Guangzhou 510120, China.
authors.forename: Qiyi
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Central Laboratory, Guangzhou Children's Hospital, Guangzhou 510120, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
authors.affiliation: South China Sea Institute of Oceanology, LED, Chinese Academy of Sciences, Guangzhou 510301, China.
authors.forename: Zaohe
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Chuyu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: South China Sea Institute of Oceanology, LED, Chinese Academy of Sciences, Guangzhou 510301, China.|Central Laboratory, Guangzhou Children's Hospital, Guangzhou 510120, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D002681:China; D004279:DNA, Viral / Q000032:analysis; D016679:Genome, Viral; D023281:Genomics; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D012141:Respiratory Tract Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D014764:Viral Proteins; C018315:hexon capsid protein, Adenovirus