Differences in the regulation of microtubule stability by the pro-rich region variants of microtubule-associated protein 4.
We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery after photobleaching analyses revealed that the interaction of MAP4-SP with the microtubules is very rapid, with a half-time of fluorescence recovery of 7 +/- 2.36 s, compared to 19.5 +/- 3.03 s in case of MAP4-LP. The dynamic interaction of MAP4-SP with microtubules in neural cells may contribute to the dynamic behaviors of extending neurites.
Hasan
Mohammad Rubayet
MR
Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan.
Jin
Mingyue
M
Matsushima
Kazuyuki
K
Miyamoto
Shigeaki
S
Kotani
Susumu
S
Nakagawa
Hiroyuki
H
D000818:Animals; D045744:Cell Line, Tumor; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*
D008869:Microtubule-Associated Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins