[Early changes in rats cervical lymph nodes after pinealectomy].
OBJECTIVE
Pineal gland acts as a reley where an anabolic activity be replaced to catabolic and conversely, to adapt a biological rhythm according to the organisme requirement. It is established that pineal extracts stimulate lymphopoiesis in the lymph node and had a radioprotective effects in lymph tissues. Lymph nodes reaction was investigated four weeks after pinealectomy, using qualitative and stereological.


METHODS
One group of animals ( Wistar rats of both sex) was shame-pinealectomized (chirurgic treatment without pineal extirpation/control) and the other was submitted to surgical ablation of pineal gland.(experimental group). The animals were kept under the same ecologic conditions and they had free access to water supplies. Animals of the same group sacrificed at the same day between 10/14 hour to equal the effects of hormonal rhythm. The paraffin section died with HE (haematoxillin-eosin), PAS/alcian blue and impregnated by Bielschowsky method. For stereological analysis multipurpose M/42 test system was applied. Volume density of nodal structure and relative cellular distribution was evaluated.


RESULTS
Cortical tissue of lymph nodes of pinealectomized rats was reduced, particularly the paracotical regions. Only in the female rats, decreased number of lymphocytes was observed in the cortex and modularly cords. Stereological analysis confirmed that increased volume density of the medulla is the result of medullar sinus dilatation. Under regressive changes of the cortical structures can be considered the involutary processes in germinal centers and reduction of the younger lymphocyte forms. Macrophage was activated and increased PAS + an inclusion was observed on the cortico/medullary junction and in the modularly cords. Reticular stroma was intensively impregnated and fibrils more coarsed and fragmented.


CONCLUSIONS
Established changes in the lymph nodes of pinealectomized a rat was analogous with that observed after treatment with the pineal extracts but in the reverse means. It was evident that the structural dissociation of the reactivity had been established. Really, at one side we have involutive changes in lymphoid population and plasma cells but progressive in phagocytes and reticular cells. It is probably that increased concentration of corticosteroides and gonadal hormones after pinealectomy is responsible for these changes.


CONCLUSIONS
Pineal gland-lymph nodes correlations established possibly via hypophyso-adreno-genital axis.
Susko
Irfan
I
Medicinski Fakultet Univerziteta U Sarajevu.
Mornjaković
Zakira
Z
Alicelebić
Selma
S
D000818:Animals; D005260:Female; D008198:Lymph Nodes / Q000473:pathology*; D008297:Male; D009333:Neck; D010870:Pineal Gland / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar