Salmonella carrier-state in hens: study of host resistance by a gene expression approach.
Salmonellosis is one of the main causes of food-borne poisoning due to the consumption of contaminated poultry products. In the flocks, Salmonella is able to persist in the digestive tract of birds for weeks without triggering any symptom. In order to identify molecules and genes involved in the mechanism of host resistance to intestinal carrier-state, two different inbred lines of laying hens were orally inoculated with Salmonella Enteritidis. Bacterial colonization and host gene expression were measured in the caecum and its sentinel lymphoid tissue, respectively. Significantly increased expression of chemokine, anti-infectious cytokine, bacterial receptor, antimicrobial mediator and particularly, defensin genes was observed in the line carrying a lower level of bacteria in the caecum. These innate immunity molecules were either constitutively or inductively highly expressed in resistant adult birds and thus present candidate genes to play an important role in the host defence against Salmonella colonization.
Sadeyen
Jean-Rémy
JR
INRA, Unité Infectiologie Animale et Santé Publique, IASP213, Centre de Recherche de Tours, 37380 Nouzilly, France.
Trotereau
Jérôme
J
Protais
Jocelyne
J
Beaumont
Catherine
C
Sellier
Nadine
N
Salvat
Gilles
G
Velge
Philippe
P
Lalmanach
Anne-Christine
AC
D000818:Animals; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary*; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D023082:Defensins / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007413:Intestinal Mucosa / Q000382:microbiology; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology; D012477:Salmonella enteritidis / Q000302:isolation & purification / Q000472:pathogenicity*; D013154:Spleen / Q000382:microbiology
D016207:Cytokines; D023082:Defensins