title: Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro.
abstract: SUZ12 is a Polycomb group protein that forms Polycomb repressive complexes (PRC2/3) together with EED and histone methyltransferase EZH2. Although the essential role of SUZ12 in regulating the activity of the PRC2/3 complexes has been demonstrated, additional function of this protein was suggested. Here, we show that SUZ12 interacts with WD-repeat protein MEP50 in vitro and in vivo. We show that the MEP50 binds histone H2A selectively among core histones, and mediates transcriptional repression of protein arginine methyltransferase PRMT5, which is known to methylate H2A and H4. These results suggest that SUZ12 might have a role in transcriptional regulation through physical interaction with MEP50 that can be an adaptor between PRMT5 and its substrate H2A.
authors.affiliation: Division of Molecular Population Genetics, Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Furuno
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Masatsugu
authors.forename: Miki
authors.initials: M
authors.lastname: Sonoda
authors.forename: Takehiko
authors.initials: T
authors.lastname: Sasazuki
authors.forename: Ken
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry* / Q000502:physiology*; D002522:Chlorocebus aethiops; D006657:Histones / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D063151:Polycomb Repressive Complex 2; D011485:Protein Binding; D011496:Protein Methyltransferases / Q000737:chemistry*; D011484:Protein-Arginine N-Methyltransferases; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D006657:Histones; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D063151:Polycomb Repressive Complex 2; D011496:Protein Methyltransferases; D011484:Protein-Arginine N-Methyltransferases; D014157:Transcription Factors; C430461:SUZ12 protein, human; C400529:PRMT5 protein, human; C478359:WDR77 protein, human