Polymeric vesicle permeability: a facile chemical assay.
We present a simple method to characterize vesicles and determine, at the same time, the membrane permeability to specific molecules. The method is based on encapsulating highly hydrophilic 3,3',3' '-phosphinidynetris-benzenesulfonic acid (PH) into vesicles and subsequently monitoring its reaction with 5,5'-dithiobis-2-nitrobenzoic acid (DTNB). We tested the method by measuring the membrane permeability of vesicles formed from a series of poly(ethylene oxide)-co-polybutylene oxide (EB) copolymers and egg yolk phosphatidylcholine. We found that the experimental data are in good agreement with calculations based on Fick's first law. We therefore quantified the DTNB permeability across vesicle membranes, finding that polymeric EB membranes have a more selective permeability toward polar molecules compared to phospholipids membranes.
Battaglia
Giuseppe
G
Department of Engineering Materials, The Kroto Research Institute, University of Sheffield, Sheffield S3 7HQ, UK. g.battaglia@sheffield.ac.uk
Ryan
Anthony J
AJ
Tomas
Salvador
S
D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D015394:Molecular Structure; D010539:Permeability; D011092:Polyethylene Glycols / Q000032:analysis* / Q000737:chemistry*
D011092:Polyethylene Glycols; D004228:Dithionitrobenzoic Acid