Effect of tributyltin on reproduction in Japanese whiting, Sillago japonica.
We examined the effect of tributyltin (TBT) on reproduction in the Japanese whiting, Sillago japonica. Mature fish were placed in indoor 500-L polyethylene tanks (five males and three females per tank) with a flow-through system and received dietary exposure to tributyltin oxide at concentrations of 2, 20, or 200 microg/g for 30 days during the active spawning period. Eggs spawned from the fish were collected daily, and the floating egg rate, larval deformity, hatchability, and viable hatch were monitored. TBT concentration in eggs of 200-microg/g group ranged from 85.0 to 159.6 ng/g in the evaluation period (days 5-30). In this period, the floating egg rate (83.2%), viable hatchability (82.2%), and total number of viable larvae (422,000 larvae per 100 g of female) were all significantly decreased in the 200-microg/g group compared with the control group (93.0%, 91.9%, and 709,000 larvae, respectively). The rate of deformity (2.6%) in the 200-microg/g group was about three times that in the control group (0.8%), although this difference was not significant. From these results, the lowest observed effect concentration of TBT in eggs on reproduction in Japanese whiting was estimated to be less than 159.6 ng/g-eggs.
Shimasaki
Yohei
Y
Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan. simasaki@agr.kyushu-u.ac.jp
Oshima
Yuji
Y
Inoue
Suguru
S
Inoue
Yoshiyuki
Y
Kang
Ik Joon
IJ
Nakayama
Kei
K
Imoto
Hisaya
H
Honjo
Tsuneo
T
D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects; D005260:Female; D008297:Male; D010063:Ovum / Q000737:chemistry / Q000187:drug effects; D010473:Perciformes / Q000502:physiology*; D012098:Reproduction / Q000187:drug effects*; D013997:Time Factors; D014220:Trialkyltin Compounds / Q000008:administration & dosage / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C005961:bis(tri-n-butyltin)oxide