title: TcRho1, the Trypanosoma cruzi Rho homologue, regulates cell-adhesion properties: evidence for a conserved function.
abstract: Rho proteins are members of the Ras superfamily of small GTPases. In higher eukaryotes these proteins play pivotal role in cell movement, phagocytosis, intracellular transport, cell-adhesion, and maintenance of cell morphology, mainly through the regulation of actin microfilaments. The GTPase TcRho1 is the only member of the Rho family described in human protozoan parasite Trypanosoma cruzi. We previously demonstrated that TcRho1 is actually required for differentiation of epimastigote to trypomastigote forms during the parasite cell cycle. In the present work, we describe cellular phenotypes induced by TcRho1 heterologous expression in NIH 3T3 fibroblasts. The NIH-3T3 lineages expressing the TcRho1-G15V and TcRho1-Q76L mutants displayed decreased levels of migration compared to the control lineage NIH-3T3 pcDNA3.1, a phenotype probably due to distinct cell-substrate adhesion properties expressed by the mutant cell lines. Accordingly, cell-substrate adhesion assays revealed that the mutant cell lines of NIH-3T3 expressing TcRho1-positive dominants constructions present enhanced substrate-adhesion phenotype. Furthermore, similar experiments with T. cruzi expressing TcRho1 mutants also revealed an enhancement of cell attachment. These results suggest that TcRho1 plays a conserved regulatory role in cell-substrate adhesion in both NIH-3T3 fibroblasts and T. cruzi epimastigotes. Taken together, our data corroborate the notion that TcRho1 may regulate the substrate-adhesion in T. cruzi, a critical step for successful progression of the parasite life cycle.
authors.affiliation: Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, CCS, UFRJ, Rio de Janeiro, Brazil.
authors.forename: Luiz Dione Barbosa
authors.initials: LD
authors.lastname: De Melo
authors.forename: Nicole
authors.initials: N
authors.lastname: Eisele
authors.forename: José Luciano
authors.initials: JL
authors.lastname: Nepomuceno-Silva
authors.forename: Ulisses Gazos
authors.initials: UG
authors.lastname: Lopes
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D006790:Host-Parasite Interactions; D006801:Humans; D008018:Life Cycle Stages; D051379:Mice; D009154:Mutation; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000737:chemistry / Q000502:physiology; D010641:Phenotype; D015800:Protozoan Proteins / Q000235:genetics / Q000502:physiology*; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000166:cytology; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D009687:Nuclear Proteins; D015800:Protozoan Proteins; C434054:Rho1 protein, Trypanosoma cruzi; D020741:rho GTP-Binding Proteins