title: Massive intestinal resection in rats fed up on glutamine: hepatic glycogen content valuation.
abstract: BACKGROUND
  Glutamine has been widely used in treatment of small bowel syndrome and its metabolic effects on the small intestine are well known, however, it has been little studied its effects on hepatic metabolism under this condition.
  
  
  OBJECTIVE
  To verify through experimental model, a glutamine based supplemental diet, administered via oral to rats submitted to massive intestinal resection, evaluating weight evolution and hepatic glycogen content.
  
  
  METHODS
  Male rats, Wistar, were allocated into three groups to undergo enterectomy. Following diets were applied: with glutamine (G group), without glutamine (NG group), and standard diet from the laboratory (R group). All animals had massive small intestine resection including ileocecal valve removal. After 20 days, all animals were sacrificed. The liver was removed to histological analysis by light microscopy. Slides were stained by periodic acid of Schiff with diastasis.
  
  
  RESULTS
  All animals lost weight from the beginning to the end of experiment. Comparing weight loss average expressed in percentage, there was no difference statistically significant on this variance. In analyzed groups, the hepatic glycogen content did not differ statistically, in the histological method evaluated.
  
  
  CONCLUSIONS
  Glutamine feeding via oral did not influence weight loss reduction of animal submitted to massive intestinal resection and did not stimulate glycogen synthesis and storage into hepatocytes.
authors.affiliation: Instituto de Assistência ao Servidor Público Estadual, University of São Paulo Medical School, São Paulo, SP, Brazil. ariney_costa@uol.com.br
authors.forename: Ariney Costa de
authors.initials: AC
authors.lastname: Miranda
authors.forename: Paulo Engler
authors.initials: PE
authors.lastname: Pinto
authors.forename: Sidney Resende
authors.initials: SR
authors.lastname: Ribeiro
authors.forename: Samson Henrique
authors.initials: SH
authors.lastname: Bromberg
authors.forename: Fábio Pinatel
authors.initials: FP
authors.lastname: Lopasso
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Irya
mesh_terms: D000818:Animals; D005973:Glutamine / Q000008:administration & dosage*; D022781:Hepatocytes / Q000378:metabolism*; D007421:Intestine, Small / Q000473:pathology / Q000601:surgery*; D008112:Liver Glycogen / Q000032:analysis*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015431:Weight Loss
chemical_list: D008112:Liver Glycogen; D005973:Glutamine