Association study of genetic variants of pro-inflammatory chemokine and cytokine genes in systemic lupus erythematosus.
BACKGROUND
Several lines of evidence suggest that chemokines and cytokines play an important role in the inflammatory development and progression of systemic lupus erythematosus. The aim of this study was to evaluate the relevance of functional genetic variations of RANTES, IL-8, IL-1alpha, and MCP-1 for systemic lupus erythematosus.


METHODS
The study was conducted on 500 SLE patients and 481 ethnically matched healthy controls. Genotyping of polymorphisms in the RANTES, IL-8, IL-1alpha, and MCP-1 genes were performed using a real-time polymerase chain reaction (PCR) system with pre-developed TaqMan allelic discrimination assay.


RESULTS
No significant differences between SLE patients and healthy controls were observed when comparing genotype, allele or haplotype frequencies of the RANTES, IL-8, IL-1alpha, and MCP-1 polymorphisms. In addition, no evidence for association with clinical sub-features of SLE was found.


CONCLUSIONS
These results suggest that the tested functional variation of RANTES, IL-8, IL-1alpha, and MCP-1 genes do not confer a relevant role in the susceptibility or severity of SLE in the Spanish population.
Sánchez
Elena
E
Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Granada, Spain. elena@ipb.csic.es
Sabio
José M
JM
Callejas
José L
JL
de Ramón
Enrique
E
Garcia-Portales
Rosa
R
García-Hernández
Francisco J
FJ
Jiménez-Alonso
Juan
J
González-Escribano
Ma Francisca
MF
Martín
Javier
J
Koeleman
Bobby P
BP
D000328:Adult; D018932:Chemokine CCL2 / Q000235:genetics; D018946:Chemokine CCL5 / Q000235:genetics; D018925:Chemokines / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007249:Inflammation / Q000235:genetics; D007375:Interleukin-1 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide
D018932:Chemokine CCL2; D018946:Chemokine CCL5; D018925:Chemokines; D007375:Interleukin-1; D016209:Interleukin-8; D007378:Interleukins