A novel approach for characterizing expression levels of genes duplicated by polyploidy.
Studying gene expression in polyploids is complicated by genomewide gene duplication and the problem of distinguishing transcript pools derived from each of the two homeologous genomes such as the A- and D-genomes of allotetraploid Gossypium. Short oligonucleotide probes designed to specifically target several hundred homeologous gene pairs of Gossypium were printed on custom NimbleGen microarrays. These results demonstrate that relative expression levels of homeologous genes may be measured by microarrays and that deviation from equal expression levels of homeologous loci may be common in the allotetraploid nucleus of Gossypium.
Udall
Joshua A
JA
Department of Ecology, Evolution and Organismal Biology, Iowa State University, Iowa 50011, USA. jaudall@iastate.edu
Swanson
Jordan M
JM
Nettleton
Dan
D
Percifield
Ryan J
RJ
Wendel
Jonathan F
JF
D001483:Base Sequence; D020440:Gene Duplication; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D003368:Gossypium / Q000235:genetics*; D046228:Microarray Analysis / Q000379:methods*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011123:Polyploidy; D016415:Sequence Alignment