Effect of dexamethasone on expression of glucocorticoid receptor in human monocyte cell line THP-1.
The effect of dexamethasone with different concentrations and different stimulating periods on the expression of glucocorticoid receptors (GRalpha, GRbeta) protein was investigated in human monocyte cell line THP-1. The cultured human monocyte line THP-1 cells were stimulated by dexamethasone with different concentrations and different periods. The expression of GRalpha and GRbeta protein was detected by Western blotting. The results showed that the expression of GRalpha and GRbeta was detected in the THP-1 cells. The quantity of GRalpha expression was reduced by dexamethasone under the same concentration with the prolongation of the stimulating periods. The quantity of GRbeta expression was increased by dexamethasone treatment in a time- and dose-dependent manner. It was concluded that dexamethasone stimulation time-dependently reduced the GRalpha expression in THP-1 cells. Dexamethasone stimulation time- and dose-dependently increased the GRbeta expression in THP-1 cells. The expression of GRalpha and GRbeta was regulated by glucocorticoid.
Li
Bo
B
Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Bai
Xiangjun
X
Wanh
Haiping
H
D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism* / Q000473:pathology; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D011965:Receptors, Glucocorticoid; C411470:glucocorticoid receptor alpha; C121943:glucocorticoid receptor beta; D003907:Dexamethasone