title: Bayesian analysis for the meiosis I non-disjunction fraction in numerical chromosomal anomalies.
abstract: The main causes of numerical chromosomal anomalies, including trisomies, arise from an error in the chromosomal segregation during the meiotic process, named a non-disjunction. One of the most used techniques to analyze chromosomal anomalies nowadays is the polymerase chain reaction (PCR), which counts the number of peaks or alleles in a polymorphic microsatellite locus. It was shown in previous works that the number of peaks has a multinomial distribution whose probabilities depend on the non-disjunction fraction F. In this work, we propose a Bayesian approach for estimating the meiosis I non-disjunction fraction F. in the absence of the parental information. Since samples of trisomic patients are, in general, small, the Bayesian approach can be a good alternative for solving this problem. We consider the sampling/importance resampling technique and the Simpson rule to extract information from the posterior distribution of F. Bayes and maximum likelihood estimators are compared through a Monte Carlo simulation, focusing on the influence of different sample sizes and prior specifications in the estimates. We apply the proposed method to estimate F. for patients with trisomy of chromosome 21 providing a sensitivity analysis for the method. The results obtained show that Bayes estimators are better in almost all situations.
authors.affiliation: Departamento de Estatística, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, MG, Brazil. loschi@est.ufmg.br
authors.forename: Rosangela H
authors.initials: RH
authors.lastname: Loschi
authors.forename: Glaura C
authors.initials: GC
authors.lastname: Franco
mesh_terms: D000465:Algorithms; D001499:Bayes Theorem; D002869:Chromosome Aberrations / Q000706:statistics & numerical data*; D003198:Computer Simulation; D004252:DNA Mutational Analysis / Q000379:methods*; D003627:Data Interpretation, Statistical; D006801:Humans; D008540:Meiosis / Q000235:genetics*; D008957:Models, Genetic; D015233:Models, Statistical; D016133:Polymerase Chain Reaction / Q000379:methods; D013269:Stochastic Processes; D014314:Trisomy / Q000235:genetics