title: Analysis of the interleukin-1 and interleukin-6 polymorphisms in patients with chronic periodontitis. A pilot study.
abstract: The aim of this study was to analyse whether the interleukin-1 (IL-1) and IL-6 gene polymorphisms were associated with the susceptibility of chronic periodontitis. Genomic DNA was obtained from 20 patients with chronic periodontitis and 31 periodontally healthy subjects. All subjects were of North European heritage. The test subjects were kept in a maintenance program after periodontal treatment but yet showing signs of recurrent disease. Genotyping of the IL-1alpha [+4845C>T], IL-1beta [-3954C>T] and IL-6 [-174G>C] polymorphisms was carried out using an allelic discrimination Assay-by-Design method on ABI PRISM 7900 Sequence Detection System. All genotypes were analyzed using the GeneMapper 2.0 software. A similar distribution of Single Nucleotide Polymorphism (SNP) was seen in both groups. Analysis by logistic regression including gender, IL-1alpha [+4845C>T], IL-1beta [-3954C>T], IL-6 [-174G>C] genotypes, the composite IL-1 genotype, the combination of the composite IL-1 genotype and the IL-6 -174G>C genotype and adjusting for smoking did not result in any statistically significant difference. SNPs in IL-1alpha [+4845C>T], IL-1beta [-3954C>T] and IL-6 [-174G>C] do not seem to increase the susceptibility to chronic periodontitis in this group of subjects.
authors.affiliation: Department of Periodontology, Centre for Oral Health Sciences, Malmö University. Henrik.Jansson@od.mah.se
authors.forename: Henrik
authors.initials: H
authors.lastname: Jansson
authors.forename: Valeriya
authors.initials: V
authors.lastname: Lyssenko
authors.forename: Asa
authors.initials: A
authors.lastname: Gustavsson
authors.forename: Kristina
authors.initials: K
authors.lastname: Hamberg
authors.forename: Björn
authors.initials: B
authors.lastname: Söderfeldt
authors.forename: Leif
authors.initials: L
authors.lastname: Groop
authors.forename: Gunilla
authors.initials: G
authors.lastname: Bratthall
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D002908:Chronic Disease; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000235:genetics* / Q000276:immunology; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6