Detection of 17alpha-hydroxyprogesterone caproate in equine plasma by gas chromatography/tandem mass spectrometry.
A method was developed for the analysis of the synthetic progestin 17alpha-hydroxyprogesterone caproate in equine plasma following its administration by intramuscular injection. The method employed a reversed-phase solid-phase extraction followed by enol-trimethylsilylation and analysis by gas chromatography/tandem mass spectrometry. The intact ester was detectable in the plasma for up to 2 weeks after a single therapeutic dose, and was found to be stable in equine whole blood for at least 2 months.
McKinney
Andrew R
AR
Australian Racing Forensic Laboratory, P.O. Box 528, Kensington, NSW 1465, Australia. amckinney@racingnsw.com.au
Suann
Craig J
CJ
Stenhouse
Allen M
AM
D000077713:17 alpha-Hydroxyprogesterone Caproate; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006736:Horses; D006908:Hydroxyprogesterones / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D007273:Injections, Intramuscular; D008297:Male; D011372:Progestins / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D006908:Hydroxyprogesterones; D011372:Progestins; D000077713:17 alpha-Hydroxyprogesterone Caproate