Injectable calcium phosphate scaffold and bone marrow graft for bone reconstruction in irradiated areas: an experimental study in rats.
The purpose of this study was to assess the possibilities for bone reconstruction of an injectable calcium phosphate scaffold (ICPS) associated with a bone marrow (BM) graft after irradiation in a rat model. External irradiation was delivered to 12 out of 27 inbred rats. Three weeks later, four osseous defects were created per animal and were kept empty or filled with either ICPS alone, BM graft alone or with a mixture of BM and ICPS. Three weeks after implantation, bone specimens were studied under light microscopy and by scanning electron microscopy. Filling irradiated defects with ICPS alone was not accompanied by the formation of new bone. The BM graft associated with ICPS significantly increased ceramic degradation (p<0.01) and bone ingrowth (p<0.01) in the irradiated areas. The results are evidence for the meaning of the BM in driving the bone repair in irradiated animals.
Lerouxel
Emmanuelle
E
INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 Place Alexis Ricordeau, F44042 Nantes Cedex, France. elerouxel@yahoo.fr
Weiss
Pierre
P
Giumelli
Bernard
B
Moreau
Anne
A
Pilet
Paul
P
Guicheux
Jérôme
J
Corre
Pierre
P
Bouler
Jean-Michel
JM
Daculsi
Guy
G
Malard
Olivier
O
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D016026:Bone Marrow Transplantation; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000008:administration & dosage*; D016025:Bone Transplantation / Q000379:methods*; D001842:Bone and Bones / Q000648:ultrastructure; D002130:Calcium Phosphates / Q000008:administration & dosage*; D002516:Ceramics / Q000378:metabolism; D005260:Female; D007267:Injections; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011917:Rats, Inbred Lew
D018786:Bone Substitutes; D002130:Calcium Phosphates; C020243:calcium phosphate