title: Tachykinin receptor expression and function in human esophageal smooth muscle.
abstract: Tachykinins are present in enteric nerves of the gastrointestinal tract and cause contraction of esophageal smooth muscle; however, the mechanisms involved are not understood. Our aim was to characterize tachykinin signaling in human esophageal smooth muscle. We investigated functional effects of tachykinins on human esophageal smooth muscle using tension recordings and isolated cells, receptor expression with reverse transcription (RT)-polymerase chain reaction (PCR) and immunoblotting, intracellular Ca2+ responses using fluorescent indicator dyes, and membrane currents with patch-clamp electrophysiology. The mammalian tachykinins [substance P and neurokinin (NK) A and NKB] elicited concentration-dependent contractions of human esophageal smooth muscle. These responses were not affected by muscarinic receptor or neuronal blockade indicating a direct effect on smooth muscle cells (SMCs). Immunofluorescence and RT-PCR identified tachykinin receptors (NK1, NK2, and NK3) on SMCs. Contraction was mediated through a combination of Ca2+ release from intracellular stores and influx through L-type Ca2+ channels. NK2 receptor blockade inhibited the largest proportion of tachykinin-evoked responses. NKA evoked a nonselective cation current (I(NSC)) with properties similar to that elicited by muscarinic stimulation. The following paradigm is suggested: tachykinin receptor binding to SMCs releases Ca2+ from stores along with activation of I(NSC), which in turn results in membrane depolarization, L-type Ca2+ channel opening, rise of Ca2+ concentration, and contraction. These studies reveal new aspects of tachykinin signaling in human esophageal SMCs. Excitatory tachykinin pathways may represent targets for pharmacological intervention in disorders of esophageal dysmotility.
authors.affiliation: Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON, Canada N6A 5C1.
authors.forename: Jason R
authors.initials: JR
authors.lastname: Kovac
authors.forename: Tom
authors.initials: T
authors.lastname: Chrones
authors.forename: Harold G
authors.initials: HG
authors.lastname: Preiksaitis
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Sims
mesh_terms: D002118:Calcium / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000187:drug effects; D002469:Cell Separation; D018679:Cholinergic Agonists / Q000494:pharmacology; D004594:Electrophysiology; D004947:Esophagus / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007473:Ion Channels / Q000187:drug effects; D009130:Muscle, Smooth / Q000378:metabolism*; D015288:Neurokinin A / Q000494:pharmacology; D015287:Neurokinin B / Q000494:pharmacology; D018040:Receptors, Neurokinin-1 / Q000096:biosynthesis; D018041:Receptors, Neurokinin-2 / Q000096:biosynthesis; D018042:Receptors, Neurokinin-3 / Q000096:biosynthesis; D018039:Receptors, Tachykinin / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D013373:Substance P / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D020746:Calcium Channels, L-Type; D018679:Cholinergic Agonists; D007473:Ion Channels; D018040:Receptors, Neurokinin-1; D018041:Receptors, Neurokinin-2; D018042:Receptors, Neurokinin-3; D018039:Receptors, Tachykinin; D013373:Substance P; D013779:Tetrodotoxin; D015288:Neurokinin A; D015287:Neurokinin B; D002118:Calcium