title: [Changes in inflammatory cascade response and its implication during cerebral ischemia/reperfusion in aged rats].
abstract: OBJECTIVE
  To investigate the changes in expression of tumor necrosis factor-alpha(TNF-alpha), vascular cellular adhesive molecule-1 (VCAM-1), intercellular adhesion molecular-1 (ICAM-1) and its mRNA expression, and the pathogenetic mechanism of cerebral ischemia/reperfusion(I/R) in elderly.
  
  
  METHODS
  Thirty six male young SD rats (5-6 months old) and 36 male aged SD rats (20-21 months old) were randomly divided into young sham operated group, young model group, aged sham operated group and aged model group, respectively. The two model groups were randomly divided into ischemia 3 hours (I 3 h), I/R 1, 3, 6, 12 days groups. There were 6 rats in each group. Focal cerebral I/R model was replicated with middle cerebral artery occlusion (MCAO). The changes of the nervous dysfunction score, the water content of cerebral constitution and the expression of TNF-alpha, VCAM-1, ICAM-1 and ICAM-1 mRNA were observed at each time point.
  
  
  RESULTS
  The expression of TNF-alpha in the aged sham operated group were higher than that of the young sham operated group. The nervous dysfunction score (I 3 h and I/R 1-12 d), the water content of cerebral constitution (I/R 1-6 d), the expression of TNF-alpha (I 3 h and I/R 1-6 d), VCAM-1 (I 3 h and I/R 1 d), ICAM-1 (I 3 h and I/R 1-6 d) and ICAM-1 mRNA (I 3 h and I/R 1-12 d) in young model group and aged model group were higher than those of the young sham operated group and the aged sham operated group respectively. The nervous dysfunction score (I 3 h, I/R 6 d), the expression of TNF-alpha (I/R 1, 3 d), VCAM-1 (I/R 3, 6 d), ICAM-1 (I 3 h, I/R 1 d) and ICAM-1 mRNA (I/R 1-6 d) in aged model group were higher than that of young model group.
  
  
  CONCLUSIONS
  The cerebral I/R injury is correlated with the up regulation of TNF-alpha, VCAM-1,ICAM-1 and its mRNA expression. The cerebral I/R injury of aged rats is more serious than that of young rats which might be associated with up regulation of TNF-alpha, adhesion molecules expression with aging.
authors.affiliation: Department of Geriatrics, Henan College of Traditional Chinese Medicine, Zhengzhou 450003, Henan, China.
authors.forename: Jian-sheng
authors.initials: JS
authors.lastname: Li
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Gao
authors.forename: You-long
authors.initials: YL
authors.lastname: Zhou
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
mesh_terms: D000367:Age Factors; D000818:Animals; D002545:Brain Ischemia / Q000209:etiology / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1