[Expression and location of UBAP1 protein associated with nasopharyngeal carcinoma].
OBJECTIVE
To analyze the expression and location of coding protein of UBAP1 gene and to understand the relationship between the expression pattern of the protein and cell carcinogenesis.


METHODS
Bioinformatics was used to analyze the protein character to provide an available clue of subsequent research. The codon frame cDNA was amplified by PCR, and subcloned into enhance green fluorescence protein (EGFP) of pEGFP-C2. The recombinant plasmid was transfected into HNE1 cells. The expression of coding protein was observed by fluorescence microscopy.


RESULTS
The expressed GFP-fusion protein generated striking green fluorescence in the cytoplasm in HNE1 cells. EGFP/UBAP1 was expressed and existed mainly in the nuclear, especially accumulated on the nuclear envelope.


CONCLUSIONS
The expression difference in HNE1 might be related to the carcinogenesis of NPC.
Zeng
Zhao-yang
ZY
Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Qian
Jun
J
Xiong
Wei
W
Zhou
Yan-hong
YH
Zhang
Wen-ling
WL
Li
Xiao-ling
XL
Tang
Ke
K
Li
Wei-fang
WF
Li
Gui-yuan
GY
D001483:Base Sequence; D002352:Carrier Proteins; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D002352:Carrier Proteins; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; C469867:UBAP1 protein, human; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins