Micellar electrokinetic capillary chromatographic method for the quantitative analysis of uricosuric and antigout drugs in pharmaceutical preparations.
A simple MEKC method is described for the separation and quantification of seven widely used uricosuric and antigout drugs, including allopurinol (AP), benzbromarone (BZB), colchicine (COL), orotic acid (OA), oxypurinol (OP), probenecid (PB), and sulfinpyrazone (SPZ). The drugs were separated in a BGE of borate buffer (45 mM; pH 9.00) with SDS (20 mM) as the micellar source and the separated drugs were directly monitored with a UV detector (214 nm). Several parameters affecting the separation and analysis of the drugs were studied. Based on the normalized peak-area ratios of the drugs to an internal standard versus the concentration of the drugs, the method is applicable to quantify BZB, COL, and SPZ (each 5-200 microM), AP, OA, OP, and PB (each 10-200 microM) with detection limits (S/N = 3, 0.5 psi, 5 s injection) in the range of 0.6-4.0 microM. The precision (RSD; n = 5) and accuracy (relative error; n = 5) of the method for intraday and interday analyses of the analytes at three levels (30, 120, and 180 microM) are below 4% (n = 3). The method was demonstrated to be suitable for the analysis of AP and COL in commercial tablets with speed and simplicity.
Kou
Hwang-Shang
HS
Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
Lin
Tsai-Pei
TP
Chung
Tang-Chia
TC
Wu
Hsin-Lung
HL
D002138:Calibration; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000592:standards; D006074:Gout Suppressants / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000032:analysis; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate; D014528:Uricosuric Agents / Q000032:analysis*
D006074:Gout Suppressants; D004364:Pharmaceutical Preparations; D014528:Uricosuric Agents; D012967:Sodium Dodecyl Sulfate