Alternative oxidase regulation in roots of Vigna unguiculata cultivars differing in drought/salt tolerance.
The alternative oxidase (Aox) was studied at different levels (transcript, protein and capacity) in response to an osmotic shock applied to roots of cowpea (Vigna unguiculata). Two cultivars of V. unguiculata were used, Vita 3 and Vita 5, tolerant and sensitive to drought/saline stress respectively. The seedlings (17-day-old) were grown in hydroponic conditions and submitted to NaCl (100 and 200 mM) or 200.67 g L(-1) PEG 6000 (iso-osmotic condition to 100 mM NaCl). The VuAox1 and VuAox2a mRNA were not detected in either cultivar under all tested conditions while the VuAox2b gene was differently expressed. In the tolerant cultivar (Vita 3), the expression of VuAox2b gene was stimulated by an osmotic stress induced by PEG which was associated with a higher amount and capacity of the Aox protein. In the same cultivar, this gene was under-expressed in salt stress conditions with poor effect on the protein level. In the sensitive cultivar (Vita 5), the transcript level of the VuAox2b was unchanged in response to PEG treatment, even though the protein and the capacity tended to increase. Upon salt stress, the VuAox2b gene was over-expressed. At 100mM NaCl, this VuAox2b gene over-expression led to a higher amount and capacity of Aox. This effect was reduced at 200 mM NaCl. Overall, these results suggest complex mechanisms (transcriptional, translational and post-translational) for Aox regulation in response to osmotic stress.
Costa
José Hélio
JH
Department of Biochemistry and Molecular Biology, Federal University of Ceará, P.O. Box 6029, 60455-760 Fortaleza Ceará, Brazil.
Jolivet
Yves
Y
Hasenfratz-Sauder
Marie-Paule
MP
Orellano
Elena Graciela
EG
da Guia Silva Lima
Maria
M
Dizengremel
Pierre
P
Fernandes de Melo
Dirce
D
D007887:Fabaceae / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D009997:Osmotic Pressure; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D000099188:Alternative Oxidase
D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D011092:Polyethylene Glycols; D012333:RNA, Messenger; D012965:Sodium Chloride; D000099188:Alternative Oxidase