title: [Effects of Rehmannia glutinosa oligosaccharides on proliferation of 3T3-L1 adipocytes and insulin resistance].
abstract: OBJECTIVE
  To investigate the influence of Rehmannia glutinosa oligosaccharides (ROS) on the proliferation of 3T3-L1 adipocytes and insulin resistance.
  
  
  METHODS
  3T3-L1 preadipocytes were cultured, the proliferation of 3T3-L1 preadipocytes was detected by MTT method. Insulin resistant 3T3-L1 adipocytes cell model was induced by dexamethasone and the change of glucose concentration in cell culture was determined after ROS treatment.
  
  
  RESULTS
  In the high glucose DMEM culture media, MTT method showed that the absorbance at 570nm of 3T3-L1 preadipocytes was increased and that of 3T3-L1 adipocytes was decreased. ROS significantly increased glucose consumption in 3T3-L1 preadipocytes and adipocytes culture in a concentration-dependent manner. ROS improved the sensitivity of 3T3-L1 adipocytes to insulin.
  
  
  CONCLUSIONS
  ROS can promote the proliferation of 3T3-L1 preadipocytes, inhibite the proliferation of 3T3-L1 adipocytes, and also, significantly improve insulin resistance induced by dexamethasone.
authors.affiliation: Life Science Institute of Lanzhou University, China.
authors.forename: Xiao-nong
authors.initials: XN
authors.lastname: Guo
authors.forename: Ru-xue
authors.initials: RX
authors.lastname: Zhang
authors.forename: Zheng-ping
authors.initials: ZP
authors.lastname: Jia
authors.forename: Mao-xing
authors.initials: MX
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007333:Insulin Resistance; D051379:Mice; D009844:Oligosaccharides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D032267:Rehmannia / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D003907:Dexamethasone