[The fine structure of chromatin in Paranosema grylli (Microsporida)].
Nucleosomes were found for the first time in the nuclear chromatin of Microsporida--organisms known among the smallest eukaryotes on Earth. Chromatin of Paranosema grylli sporoplasm was studied by Miller's technique. On low ionic-strength cell spreads, this chromatin was represented by 10 nm nucleosome filaments, 20 nm filaments, and "smooth" (nucleosome-free) filaments of 3-4 nm in diameter. Nucleosome filaments display structural heterogeneity seen as irregular arrangement of nucleosome particles along the filament length. Different nucleosome filaments show 13-30 nucleosomes per 1 microm with the length of linker DNA ranging from 10 to 45 nm. The present results suggest that microsporidian chromatin is weakly condensed. Only lower-order chromatin packaging levels displayed some structural peculiarities.
Bobyleva
N N
NN
Nasonova
E S
ES
Skarlato
S O
SO
D002843:Chromatin / Q000737:chemistry / Q000648:ultrastructure*; D004271:DNA, Fungal / Q000648:ultrastructure*; D008854:Microscopy, Electron; D016816:Microsporida / Q000235:genetics*; D009707:Nucleosomes / Q000648:ultrastructure
D002843:Chromatin; D004271:DNA, Fungal; D009707:Nucleosomes