U1 and U2 small nuclear RNA genetic linkage: a novel molecular tool for identification of six sole species (Soleidae, Pleuronectiformes).
We evaluated the usefulness of a genetic linkage between the U1 and U2 small nuclear RNAs for species identification. Six soles belonging to the genera Solea, Dicologlossa, and Microchirus were studied. A simple methodology based on two single PCRs is described. Reproducible band profiles were generated for all samples. This rapid and discriminatory molecular method is highly promising for determining the authenticity of sole fillets in the food industry.
Manchado
Manuel
M
CIFPA "El Toruño", I.F.A.P.A. C.I.C.E. (Junta de Andalucía). Camino tiro de pichón s/n, 11500 El Puerto de Santa María, Cadiz, Spain. manuel.manchado.ext@juntadeandalucia.es
Rebordinos
Laureana
L
Infante
Carlos
C
D000818:Animals; D004247:DNA / Q000032:analysis / Q000737:chemistry; D005412:Flatfishes / Q000145:classification* / Q000235:genetics*; D019649:Food Industry; D008040:Genetic Linkage; D016133:Polymerase Chain Reaction; D012342:RNA, Small Nuclear / Q000235:genetics*; D015203:Reproducibility of Results
D012342:RNA, Small Nuclear; C405483:U1 small nuclear RNA; C405484:U2 small nuclear RNA; D004247:DNA