Treatment and transfer of emphysema by a new bone marrow transplantation method from normal mice to Tsk mice and vice versa.
We have recently established a new bone marrow transplantation (BMT) method in which bone marrow cells are injected into the intrabone marrow (IBM). In the present study, we used an animal model for emphysema (tight-skin [Tsk] mouse) to examine whether IBM-BMT could be used to treat emphysema in Tsk mice. IBM-BMT was carried out from C3H mice into Tsk mice (8-10 weeks old) that had already shown emphysema. Six months after transplantation, the lungs of all the Tsk mice treated with IBM-BMT [C3H-->Tsk] showed similar structures to those of normal mice, whereas the [Tsk-->Tsk] mice showed emphysema, as seen in age-matched Tsk mice. Next, we attempted to transfer emphysema from Tsk mice to C3H mice by IBM-BMT. Six months after IBM-BMT, the [Tsk-->C3H] mice showed emphysema. These results strongly suggest that emphysema in Tsk mice originates from defects of stem cells in the bone marrow.
Adachi
Yasushi
Y
First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan, 570-0023.
Oyaizu
Haruki
H
Taketani
Shigeru
S
Minamino
Keizo
K
Yamaguchi
Kazuyuki
K
Shultz
Leonard D
LD
Iwasaki
Masayoshi
M
Tomita
Minoru
M
Suzuki
Yasuhiro
Y
Nakano
Keiji
K
Koike
Yasushi
Y
Yasumizu
Ryoji
R
Sata
Makoto
M
Hirama
Noriyuki
N
Kubota
Isao
I
Fukuhara
Shirou
S
Ikehara
Susumu
S
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods*; D046528:Chimerism; D004646:Emphysema / Q000628:therapy*; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D006413:Hematopoietic System / Q000166:cytology; D008168:Lung / Q000166:cytology / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011505:Protein-Tyrosine Kinases / Q000172:deficiency*; D012867:Skin / Q000166:cytology / Q000473:pathology
D011505:Protein-Tyrosine Kinases; C080813:emt protein-tyrosine kinase