Relationship between p21 and p53 expression, human papilloma virus infection and malignant transformation in sinonasal-inverted papilloma.
OBJECTIVE
To identify the relationship between p21 and p53 expression, human papilloma virus (HPV) infection and malignant transformation in sinonasal-inverted papilloma.


METHODS
Nasal tissues, exophytic papilloma, inverted papilloma (IP) with dysplasia, IP with carcinoma and invasive squamous cell carcinoma (SCC) were stained with the monoclonal antibodies p21 and p53. In-situ hybridisation for HPV DNA was also carried out for types 6/11, 16/18 and 31/33.


RESULTS
Significant increased staining of p21 and p53 was observed in IP with severe dysplasia, IP with carcinoma and invasive carcinoma compared with control nasal mucosa. A significant increase of dysplasia was observed in IP in the HPV 6/11 and 16/18-positive group, compared with the HPV 6/11 and 16/18-negative group. Significant decrease in expression of p21 and p53 was observed in HPV 16/18-positive IP compared with HPV 16/18-negative IP.


CONCLUSIONS
Our data raise the possibility that testing for p21, p53 and HPV may help to screen out papilloma lesions with a potential for dysplasia or carcinoma.
Katori
H
H
Department of Otolaryngology, Yokohama City University Medical Center, Yokohama, Japan. hhkatori@yahoo.co.jp
Nozawat
A
A
Tsukuda
M
M
D016022:Case-Control Studies; D049109:Cell Proliferation; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D004279:DNA, Viral; D005260:Female; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009669:Nose Neoplasms / Q000473:pathology* / Q000821:virology; D009858:Oncogenic Viruses; D018308:Papilloma, Inverted / Q000473:pathology* / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D010255:Paranasal Sinus Neoplasms / Q000473:pathology* / Q000821:virology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D050759:Cyclin-Dependent Kinase Inhibitor p21; D004279:DNA, Viral; D016159:Tumor Suppressor Protein p53