title: Different human papillomavirus 16/18 infection in Chinese non-small cell lung cancer patients living in Wuhan, China.
abstract: BACKGROUND
  Inconsistency in the prevalence of infection by human papillomavirus (HPV) in lung cancer patients was found between different countries with racial and geographic variations. Our previous reports have indicated that a high-risk HPV 16/18 DNA was frequently detected in Chinese lung cancer patients living in Taichung, Taiwan (Cheng et al. Cancer Res. 2001;61:2799-803). Thus, we conducted this study to verify whether there was a similar HPV 16/18 infection prevalence in lung cancer patients from Wuhan, China.
  
  
  METHODS
  To reduce the false positive HPV detection, the paraffin sections of 73 lung tumors and 34 non-cancer controls from Wuhan, China were collected for detection of the presence of HPV 16/18 DNA by in situ hybridization (ISH).
  
  
  RESULTS
  Our results showed that the rates of HPV 16 and/or 18 infections in patients with lung tumors were significantly higher than in 34 non-cancer control subjects (26.0 versus 2.8% for HPV 16, P = 0.030; 23.3 versus 5.7% for HPV 18, P = 0.031; 27.7 versus 5.9% for HPV 16 or 18, P = 0.003) with a similar infection frequency of HPV 16 and 18 types in lung tumors. This result indicated that HPV 16/18 infection may be associated with lung cancer development in Chinese patients from Wuhan, China. Further statistical analyses revealed that HPV 16 or 18 infection was not correlated with any clinico-pathological parameter studied, including age, gender, smoking status, tumor type, tumor stage and tumor grades. Interestingly, smoking and male patients had a higher prevalence of HPV 16, although not reaching a statistical significance, compared with non-smoking and female patients, respectively (33.3% for smokers versus 20.0% non-smokers; 33.3% for male versus 17.6% for female). As compared with the HPV 16/18 infection in Taiwan, Chinese patients with lung cancer from Wuhan had a different HPV 16/18 infection prevalence.
  
  
  CONCLUSIONS
  Difference in HPV 16/18 infection in lung cancer patients from Wuhan, China and Taichung, Taiwan suggests that HPV 16/18 might play a different role in lung cancer development among Chinese living in different areas.
authors.affiliation: Department of Pathology, Wuhan University Medical School, Hubei Province, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Fei
authors.forename: Jiong
authors.initials: J
authors.lastname: Yang
authors.forename: Wei-Chung
authors.initials: WC
authors.lastname: Hsieh
authors.forename: Jeng-Yuan
authors.initials: JY
authors.lastname: Wu
authors.forename: Tzu-Chin
authors.initials: TC
authors.lastname: Wu
authors.forename: Yih-Gang
authors.initials: YG
authors.lastname: Goan
authors.forename: Huei
authors.initials: H
authors.lastname: Lee
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Cheng
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000821:virology; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology / Q000821:virology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics; D052161:Human papillomavirus 18 / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology*; D015995:Prevalence; D012907:Smoking; D013624:Taiwan / Q000453:epidemiology
chemical_list: D004279:DNA, Viral