title: In situ profiling and quantification of cytokines released during ultraviolet B-induced inflammation by combining dermal microdialysis and protein microarrays.
abstract: In skin, an evolving inflammatory or immune response is triggered by early release of a cytokine cascade into the extracellular space. Investigation of extracellular cytokine secretion in situ has been limited by low cut-off filtering membranes and sample volume size and the inability to monitor changes in cytokine protein levels in real-time in situ. Here, we combine for the first time the methods of intradermal microdialysis and antibody protein arraying to profile the early cascade of multiple cytokines in a complex inflammatory response exemplified by ultraviolet B (UVB)-induced inflammation. We observed significant differences of the cytokine and growth factor responses after tissue injury by catheter placement and UVB-induced inflammation. UVB irradiation initiates a rapid proinflammatory response followed by a mixed TH1/TH2 response in which ultimately TH2 cytokines IL-4 and IL10 predominated after 24 h. This most likely indicates the termination and self limitation of the inflammatory response. We conclude that the combination of dermal microdialysis and protein microarray offers a powerful tool to analyze in real-time the complex and rapidly changing interstitial protein milieu during cutaneous inflammatory responses.
authors.affiliation: Department of Dermatology, Venerology and Allergology, University of Leipzig, Germany. marco.averbeck@medizin.uni-leipzig.de
authors.forename: Marco
authors.initials: M
authors.lastname: Averbeck
authors.forename: Simone
authors.initials: S
authors.lastname: Beilharz
authors.forename: Matthias
authors.initials: M
authors.lastname: Bauer
authors.forename: Carl
authors.initials: C
authors.lastname: Gebhardt
authors.forename: Alexandra
authors.initials: A
authors.lastname: Hartmann
authors.forename: Klaus
authors.initials: K
authors.lastname: Hochleitner
authors.forename: Friederike
authors.initials: F
authors.lastname: Kauer
authors.forename: Ursula
authors.initials: U
authors.lastname: Voith
authors.forename: Jan C
authors.initials: JC
authors.lastname: Simon
authors.forename: Christian
authors.initials: C
authors.lastname: Termeer
mesh_terms: D000328:Adult; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D017551:Microdialysis / Q000379:methods*; D009389:Neovascularization, Pathologic / Q000209:etiology; D040081:Protein Array Analysis / Q000379:methods*; D011855:Radiodermatitis / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D018417:Th1 Cells / Q000276:immunology / Q000528:radiation effects; D018418:Th2 Cells / Q000276:immunology / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D016207:Cytokines