title: PINK1 protein in normal human brain and Parkinson's disease.
abstract: Parkinson's disease is a common incurable neurodegenerative disease whose molecular aetiology remains unclear. The identification of Mendelian genes causing rare familial forms of Parkinson's disease has revealed novel proteins and pathways that are likely to be relevant in the pathogenesis of sporadic Parkinson's disease. Recently, mutations in a novel gene, PINK1, encoding a 581 amino acid protein with both mitochondrial targeting and serine/threonine kinase domains, were identified as a cause of autosomal recessive parkinsonism. This provided important evidence for the role of the mitochondrial dysfunction and kinase pathways in neurodegeneration. In this study, we report the first characterization of the PINK1 protein in normal human and sporadic Parkinson's brains, in addition to Parkinson's cases with heterozygous PINK1 mutations. The possible role of the PINK1 protein was also assessed in a number of neurodegenerative diseases characterized by proteinaceous inclusions. For these studies, rabbit polyclonal antibodies were raised against two peptide sequences within the N-terminal hydrophilic loops of PINK1 protein. Using immunohistochemistry and western blotting we were able to demonstrate that PINK1 is a ubiquitous protein expressed throughout the human brain and it is found in all cell types showing a punctate cytoplasmic staining pattern consistent with mitochondrial localization. Fractionation studies of human and rat brain confirm that PINK1 is localized to the mitochondrial membranes. In addition, we show that PINK1 is detected in a proportion of Lewy bodies in cases of sporadic Parkinson's disease and Parkinson's disease associated with heterozygous mutations in the PINK1 gene, which are clinically and pathologically indistinguishable from the sporadic cases. PINK1 was absent in cortical Lewy bodies, in neurofibrillary tangles in Alzheimer's disease, progressive supranuclear palsy and corticobasal degeneration, and in the glial and neuronal alpha-synuclein positive inclusions in multiple system atrophy. These studies provide for the first time in vivo morphological and biochemical evidence to support a mitochondrial localization of PINK1 and underpin the significance of mitochondrial dysfunction in the pathogenesis of nigral cell degeneration in Parkinson's disease.
authors.affiliation: Department of Molecular Neuroscience, Institute of Neurology University College London, London, UK.
authors.forename: S
authors.initials: S
authors.lastname: Gandhi
authors.forename: M M K
authors.initials: MM
authors.lastname: Muqit
authors.forename: L
authors.initials: L
authors.lastname: Stanyer
authors.forename: D G
authors.initials: DG
authors.lastname: Healy
authors.forename: P M
authors.initials: PM
authors.lastname: Abou-Sleiman
authors.forename: I
authors.initials: I
authors.lastname: Hargreaves
authors.forename: S
authors.initials: S
authors.lastname: Heales
authors.forename: M
authors.initials: M
authors.lastname: Ganguly
authors.forename: L
authors.initials: L
authors.lastname: Parsons
authors.forename: A J
authors.initials: AJ
authors.lastname: Lees
authors.forename: D S
authors.initials: DS
authors.lastname: Latchman
authors.forename: J L
authors.initials: JL
authors.lastname: Holton
authors.forename: N W
authors.initials: NW
authors.lastname: Wood
authors.forename: T
authors.initials: T
authors.lastname: Revesz
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002254:Carbonates / Q000494:pharmacology; D005260:Female; D006579:Heterozygote; D006801:Humans; D007124:Immunoenzyme Techniques; D020961:Lewy Body Disease / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism; D019578:Multiple System Atrophy / Q000378:metabolism; D009154:Mutation; D010300:Parkinson Disease / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013367:Submitochondrial Particles / Q000187:drug effects / Q000378:metabolism; D000099202:PTEN-Induced Putative Kinase
chemical_list: D002254:Carbonates; D011494:Protein Kinases; D000099202:PTEN-Induced Putative Kinase; C005686:sodium carbonate