Anti-CXCR3 staining is useful for detecting human cutaneous and mucosal mast cells.
Human synovial mast cells (MC) can be immunolabelled with antihuman CXCR3 antibody (Ab) (clone 49801). We have investigated whether cutaneous and mucosal MC are stained with anti-CXCR3 Ab in paraffin-embedded sections. Immunohistochemical staining and immunofluorescence double staining assays were performed with anti-CXCR3, anti-tryptase, and anti-chymase Ab using normal skin, psoriatic skin lesions, and normal colon. When compared with tryptase and chymase staining, 100% of the cutaneous and 98% of the mucosal MC were positive for CXCR3. Anti-CXCR3 staining is a useful marker for human cutaneous and mucosal MC in paraffin-embedded sections.
Fukiwake
Noriko
N
Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. norikof@dermatol.med.kyushu-u.ac.jp
Moroi
Yoichi
Y
Imafuku
Shinichi
S
Masuda
Teiichi
T
Kokuba
Hisashi
H
Furue
Masutaka
M
Urabe
Kazunori
K
D000911:Antibodies, Monoclonal; D003106:Colon / Q000166:cytology; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D008407:Mast Cells / Q000378:metabolism*; D016612:Paraffin Embedding; D011565:Psoriasis / Q000473:pathology*; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000276:immunology*; D012867:Skin / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods*
D000911:Antibodies, Monoclonal; C515187:CXCR3 protein, human; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine