Bacteriological and electron microscopic examination of primary intrahepatic stones.
BACKGROUND
Primary intrahepatic cholelithiasis is usually combined with biliary tract infection. This research was undertaken to investigate the relationship between intrahepatic stones and biliary tract infection.


METHODS
Thirty-five bile samples and 30 stones specimens were cultured for bacteria and 12 stones specimens were examined with a scan electron microscope (SEM) or a transmission electron microscope (TEM).


RESULTS
94.2% bile samples and 96.7% stones specimens were positive in bacteria culture. Bacteria were found in stones under SEM and TEM.


CONCLUSIONS
Bacteria in stones are associated with the infection of the biliary tract.
Wu
Shuo-Dong
SD
Department of General Surgery, Second Affiliated Hospital (Shengjing Hospital), China Medical University, Shenyang 110004, China. wushuodong@hotmail.com
Yu
Hong
H
Sun
Ji-Mei
JM
D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology; D001660:Biliary Tract Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000601:surgery; D005260:Female; D042882:Gallstones / Q000737:chemistry* / Q000453:epidemiology / Q000382:microbiology* / Q000648:ultrastructure; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000302:isolation & purification; D006801:Humans; D015994:Incidence; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index