title: CMV DNA detection in dried blood spots for diagnosing congenital CMV infection in Japan.
abstract: Human cytomegalovirus (CMV) is a leading congenital infectious agent in developed countries. In the past, the incidence of congenital infection has been rather low in Japan because a high seroprevalence of CMV present in young women. However, this seroprevalence has been decreasing in recent years, so that the incidence of congenital CMV infection in Japanese neonates may increase and approach the level seen in other developed countries. The method was used for detecting CMV DNA reported by Barbi et al. [Barbi et al. (1996): Clin Diagn Virol 6:27-32] using a dried blood spot on filter paper, to diagnose congenital CMV infection in Japanese neonates. This method is effective and less laborious than virus isolation both for epidemiological studies and for identifying asymptomatic infected babies. Japanese neonates (1,176) were examined; two of who were asymptomatic were found to be infected.
authors.affiliation: Department of Paediatrics, Osaka University, Graduate School of Medicine, Osaka, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yamagishi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Miyagawa
authors.forename: Kazuko
authors.initials: K
authors.lastname: Wada
authors.forename: Sayuri
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Hitomi
authors.initials: H
authors.lastname: Arahori
authors.forename: Arihiro
authors.initials: A
authors.lastname: Tamura
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Takahisa
authors.initials: T
authors.lastname: Kanekiyo
authors.forename: Junji
authors.initials: J
authors.lastname: Sashihara
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yoda
authors.forename: Mikiya
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ozono
mesh_terms: D001483:Base Sequence; D001800:Blood Specimen Collection; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000175:diagnosis* / Q000453:epidemiology / Q000635:transmission / Q000821:virology; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious
chemical_list: D004279:DNA, Viral