[Protective effect of lidocaine on injury alveolar Type II cells induced by LPS in adult rats].
OBJECTIVE
To investigate the effect of lidocaine on LPS induced apoptosis of cultured adult rat alveolar Type II (AT-II) cells.


METHODS
Cultured cells were exposed to LPS and lidocaine for 24 hours. Apoptosis and necrosis rates of cells were detected by flow cytometry and electron microscope. The activity of lactic dehydrogenase (LDH) was analyzed by using LDH kits.


RESULTS
LPS induced the AT-II cell injuries by increasing not only the necrosis and apoptosis rates but also the LDH release of cultured AT-II in vitro. Lidocaine decreased the necrosis and apoptosis rates of AT-II cells.


CONCLUSIONS
Lidocaine can directly inhibit the apoptosis and necrosis induced by LPS in cultured AT-II cells.
Xu
Dao-miao
DM
Department of ICU, Xiangya Hospital, Central South University, Changsha, China. xudaomiao@medmail.com.cn
Ming
Guang-feng
GF
Wu
Xiao-ying
XY
Chen
Sheng-xi
SX
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000473:pathology*; D005434:Flow Cytometry; D045022:Lactate Dehydrogenases / Q000378:metabolism; D008012:Lidocaine / Q000494:pharmacology*; D008070:Lipopolysaccharides; D009336:Necrosis; D020011:Protective Agents / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008070:Lipopolysaccharides; D020011:Protective Agents; D008012:Lidocaine; D045022:Lactate Dehydrogenases