title: Simultaneous liquid chromatography determination of polyamines and arylalkyl monoamines.
abstract: The diamines putrescine (PUT) and diaminopropane (DAP), the polyamines spermidine (SPD) and spermine (SPM), and the arylalkyl amines phenethylamine (PEA), tyramine (TYR), dopamine (DA), and salsolinol (SAL) were dansylated and baseline separated by LC using a Waters ODS-2 column. The dansyl derivatives were detected by fluorescence (lambda(ex): 337 nm; lambda(em): 520 nm). Besides the amine function, the phenolic OH groups of TYR, DA, and SAL were also dansylated (LC-MS, formation of N,O-didansyl [TYR] and N,O,O'-tridansyl derivatives [DA and SAL]). Calibration curves revealed response factors being appreciably lower for (N,O-didansyl) aminophenol TYR and (N,O,O'-tridansyl) DA and SAL than for N-dansylamines. However, the method is suitable as a cheap alternative to LC-MS for the simultaneous determination of polyamines and arylalkyl amines of large quantities of samples.
authors.affiliation: Laboratory of Functional Biology, Katholieke Universiteit Leuven, B-3001 Leuven, Belgium. jan.genus@bio.kuleuven.be
authors.forename: Jan M C
authors.initials: JM
authors.lastname: Geuns
authors.forename: Marta Ledo
authors.initials: ML
authors.lastname: Orriach
authors.forename: Rony
authors.initials: R
authors.lastname: Swennen
authors.forename: Guoyu
authors.initials: G
authors.lastname: Zhu
authors.forename: Bart
authors.initials: B
authors.lastname: Panis
authors.forename: Frans
authors.initials: F
authors.lastname: Compernolle
authors.forename: Mark
authors.initials: M
authors.lastname: Van der Auweraer
mesh_terms: D000588:Amines / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D003619:Dansyl Compounds / Q000032:analysis / Q000737:chemistry; D005470:Fluorometry / Q000379:methods; D011073:Polyamines / Q000032:analysis* / Q000737:chemistry
chemical_list: D000588:Amines; D003619:Dansyl Compounds; D011073:Polyamines