Repopulation of the mesothelial monolayer during long-term experimental peritoneal dialysis.
BACKGROUND
Repopulation of the mesothelial monolayer after focal exfoliation, having the monolayer in vivo and in situ exposed to dialysis solutions, has not been thoroughly investigated. This study describes repopulation of a 'doughnut' like mesothelial ring exfoliated from the anterior liver surface of rats.


METHODS
Animals were divided into 5 groups of 20 rats each. Group 1 - control unexposed animals: mesothelial cell imprints were taken after 1 (5 rats), 5 (5 rats), and 15 (10 rats) days following the procedure of exfoliation. Group 2 - sham injected animals. Group 3 - rats IP injected once a day, during 30 consecutive days with Hank's balanced salt solution. Groups 4 and 5 - same experimental protocol, but injecting 4.25% glucose single bag or 7.5% Icodextrin PDF. Imprints and/or biopsies were taken after a recovery period of 15 days, counted from the last IP injection.


RESULTS
Density distribution of mesothelial cells in group 2 was not significantly different from that seen in unexposed rats, whereas that seen in group 3 Hank's balanced salt solution was marginally but significantly lower (p < 0.05) from that seen in controls. Eighty five percent of rats injected with 4.25% glucose developed fibrous adhesions, binding up together the exfoliated liver surface and the diaphragmatic muscle. For Icodextrin treated rats, the prevalence of fibrous adhesions was 95%.


CONCLUSIONS
The 'doughnut' experimental model appears as a promising tool for in vivo and in situ investigation of mesothelial repopulation. Both osmotic agents substantially restrain mesothelial repopulation, leading to repair by connective tissue.
Gotloib
Lazaro
L
Department of Nephrology and the Research Center for Experimental Nephrology, Ha'Emek Medical Center, Afula, Israel.
Wajsbrot
Valery
V
Shostak
Avshalom
A
Khrizman
Vladimir
V
D000818:Animals; D015314:Dialysis Solutions; D004847:Epithelial Cells / Q000473:pathology*; D005947:Glucose / Q000378:metabolism; D006706:Homeostasis; D010530:Peritoneal Dialysis / Q000009:adverse effects*; D010537:Peritoneum / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D015314:Dialysis Solutions; D005947:Glucose