Development of a solvent-free, solid-phase in vitro bioassay using vertebrate cells.
Miniaturized bioassays offer many advantages in exploring the toxic potential of chemicals, including small sample volumes and compatibility with high-throughput screening. One problem common to miniaturized systems, however, is the loss of test chemicals because of sorption. The idea of the current study was to use the sorption phenomenon in a positive way. It was found that contaminants sorbed to the growth surface in wells of tissue-culture plates or to the surface of selected sorbent bead materials are available to vertebrate cells growing in direct contact with the contaminant-coated surface. The use of beads provided more flexibility with regard to surface area, materials, and assay format. Biosilon, a bead cell-culture carrier made of polystyrene, was found to be most suitable. It supported cell adherence and allowed the detection of reproducible dose-response curves of an increase in cytochrome CYP1A enzyme activity by sorbed polycyclic aromatic hydrocarbons in the rainbow trout (Oncorhynchus mykiss) liver cell line, RTL-W1. The resulting bead assay provides a miniaturized, solvent-free exposure system. Potential future applications include the coupling to environmental sampling, in which the bead material is used as solid receiving phase before serving as a surface for vertebrate cells to attach and respond.
Bopp
Stephanie K
SK
Department of Cell Toxicology, UFZ-Centre for Environmental Research Leipzig-Halle in the Helmholtz Association, Permoserstrasse 15, 04318 Leipzig, Germany.
Bols
Niels C
NC
Schirmer
Kristin
K
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D000327:Adsorption; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D017686:Oncorhynchus mykiss / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D011137:Polystyrenes / Q000737:chemistry; D012997:Solvents
D011084:Polycyclic Aromatic Hydrocarbons; D011137:Polystyrenes; D012997:Solvents; C024075:styrofoam; D015660:7-Alkoxycoumarin O-Dealkylase