title: [Development of tissue microarray technology (TMA) for immunohistochemical study of molecular expression profiling in prostate cancer (part 1)].
abstract: Tissue microarray technology (TMA) is nowadays considered as a powerful tool for the high-throughput analysis of molecular expression pattern of cancer. In this manuscript we show the experience of both groups in the design and building of a TMA for the study of protein expression pattern of prostatecancer as well as a summary of the technical points to analyze the results obtained with this technology. Today, different data generated by the immunostained tissues are studied to achieve a molecular profile in different clinical scenarios.
authors.affiliation: Servicio y Cátedra de Urología, Laboratorio de Uro-Patología Aplicada, Hospital del Mar, Barcelona. jlorente@imas.imim.es
authors.forename: J A
authors.initials: JA
authors.lastname: Lorente Garín
authors.forename: J
authors.initials: J
authors.lastname: Lloreta Trull
authors.forename: C
authors.initials: C
authors.lastname: Allepuz Losa
authors.forename: L
authors.initials: L
authors.lastname: Plaza Mas
authors.forename: L A
authors.initials: LA
authors.lastname: Rioja Sanz
authors.forename: A
authors.initials: A
authors.lastname: Gelabert Mas
mesh_terms: D004867:Equipment Design; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D046228:Microarray Analysis / Q000295:instrumentation*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*