Observation of all the components of elastic constants using tetragonal hen egg-white lysozyme crystals dehydrated at 42% relative humidity.
Success in measuring transverse sound velocity allowed us to determine, for the first time, all six elastic constants of a protein crystal. An ultrasonic pulse-echo method was used to perform sound velocity measurements on tetragonal hen egg-white (HEW) lysozyme crystals that were partially dehydrated at 42% relative humidity. The measurements were performed using the (110), (101), and (001) crystallographic faces. Thus, all six elastic constants of the dehydrated tetragonal HEW lysozyme crystals were determined: C11=C22=12.44 GPa, C12=7.03 GPa, C13=C23=8.36 GPa, C33=12.79 GPa, C44=C55=2.97 GPa, and C66=2.63 GPa. In addition, for the hydrated crystals, the longitudinal sound velocities along the [110] direction and the direction normal to the (101) face were measured. From these results, all the components of elastic constants in the hydrated crystals were extrapolated.
Koizumi
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H
Graduate School of Integrated Science, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan.
Tachibana
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M
Kojima
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K
D000818:Animals; D002645:Chickens; D003198:Computer Simulation; D003460:Crystallization / Q000379:methods*; D003461:Crystallography / Q000379:methods*; D003890:Desiccation; D004548:Elasticity; D008956:Models, Chemical; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000032:analysis / Q000737:chemistry; D009113:Muramidase / Q000032:analysis* / Q000737:chemistry*; D011487:Protein Conformation; D013314:Stress, Mechanical; D014867:Water / Q000737:chemistry*
D046912:Multiprotein Complexes; D014867:Water; C421864:hen egg lysozyme; D009113:Muramidase