[Construction of two hepatocellular carcinoma cell models for the expression of HBV X gene with different selection characteristics].
OBJECTIVE
To construct 2 hepatocellular carcinoma (HCC) cell models for the expression of HBV X gene with different selection characteristics.


METHODS
HepG2 HCC cells were infected with eukaryotic expression vectors with HBV X gene, pCEP4-X, and pcDNA3. 1 (+)-X. Single cell clone was selected by hygromycin and neomycin. After propagating culture for certain periods, the HBV X gene expression was identified by PCR, RT-PCR, and Western blot.


RESULTS
Single HCC cell clone with HBV X gene transferred resistant to hygromycin and neomycin was selectively cultured, and the cells could be propagated for certain periods. PCR, RT-PCR, and Western blot identified the expression of HBV X gene.


CONCLUSIONS
Two HCC cell models for the expression of HBV X gene with different selection characteristics have been successfully constructed.
He
Xing-e
XE
Liver Disease Research Center, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Lei
Jian-hua
JH
Yang
Xu
X
Wang
Wen-long
WL
Luo
Hong-yu
HY
Liang
Jun
J
D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D005057:Eukaryotic Cells / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008954:Models, Biological; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins
D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein