title: Maltose and maltodextrin utilization by Bacillus subtilis.
abstract: Bacillus subtilis can utilize maltose and maltodextrins that are derived from polysaccharides, like starch or glycogen. In this work, we show that maltose is taken up by a member of the phosphoenolpyruvate-dependent phosphotransferase system and maltodextrins are taken up by a maltodextrin-specific ABC transporter. Uptake of maltose by the phosphoenolpyruvate-dependent phosphotransferase system is mediated by maltose-specific enzyme IICB (MalP; synonym, GlvC), with an apparent K(m) of 5 microM and a V(max) of 91 nmol . min(-1) . (10(10) CFU)(-1). The maltodextrin-specific ABC transporter is composed of the maltodextrin binding protein MdxE (formerly YvdG), with affinities in the low micromolar range for maltodextrins, and the membrane-spanning components MdxF and MdxG (formerly YvdH and YvdI, respectively), as well as the energizing ATPase MsmX. Maltotriose transport occurs with an apparent K(m) of 1.4 microM and a V(max) of 4.7 nmol . min(-1) . (10(10) CFU)(-1).
authors.affiliation: Lehrstuhl für Mikrobiologie, Fachbereich Biologie der Universität Konstanz, Universitätsstrasse 10, M605, D-78457 Konstanz, Germany. stefan.schoenert@gmx.de
authors.forename: Stefan
authors.initials: S
authors.lastname: Schönert
authors.forename: Sabine
authors.initials: S
authors.lastname: Seitz
authors.forename: Holger
authors.initials: H
authors.lastname: Krafft
authors.forename: Eva-Anne
authors.initials: EA
authors.lastname: Feuerbaum
authors.forename: Iris
authors.initials: I
authors.lastname: Andernach
authors.forename: Gabriele
authors.initials: G
authors.lastname: Witz
authors.forename: Michael K
authors.initials: MK
authors.lastname: Dahl
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001692:Biological Transport; D005838:Genotype; D007700:Kinetics; D008320:Maltose / Q000378:metabolism*; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010957:Plasmids; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D015183:Restriction Mapping
chemical_list: D011134:Polysaccharides; D008320:Maltose; C008315:maltodextrin; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System