A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli.
Although Escherichia coli is in wide use for preparative protein expression, problems with the folding of the recombinant gene product and protein aggregation are frequently encountered. Apart from cytoplasmic expression, this is also true for secretion into the bacterial periplasm, the method of choice for the production of proteins that carry structural disulfide bonds. Here we report the construction of the helper plasmid pTUM4, which effects overexpression of four established periplasmic chaperones and folding catalysts: the thiol-disulfide oxidoreductases DsbA and DsbC that catalyze the formation and isomerization of disulfide bridges and the peptidyl-prolyl cis/trans-isomerases with chaperone activity, FkpA and SurA. pTUM4 carries a p15a origin of replication and a chloramphenicol resistance gene and, thus, it is compatible with many conventional expression vectors that use the ColEI origin and an ampicillin resistance. Its positive effects on the yield of soluble recombinant protein and the homogeneity of disulfide pattern are illustrated here using the human plasma retinol-binding protein as well as the extracellular carbohydrate recognition domain of the dendritic cell membrane receptor DC-SIGN. Hence, pTUM4 represents a novel helper vector which complements existing cytosolic chaperone coexpression plasmids and should be useful for the functional secretion of various recombinant proteins with hampered folding efficiency.
Schlapschy
Martin
M
Lehrstuhl für Biologische Chemie, Technische Universität München, 85350 Freising-Weihenstephan, Germany.
Grimm
Sebastian
S
Skerra
Arne
A
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D020104:Immunophilins / Q000235:genetics / Q000378:metabolism; D037181:Lectins, C-Type / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D019696:Peptidylprolyl Isomerase / Q000235:genetics / Q000378:metabolism; D019897:Periplasm / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D019704:Protein Disulfide-Isomerases / Q000235:genetics / Q000378:metabolism; D017510:Protein Folding; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D012177:Retinol-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D054839:Retinol-Binding Proteins, Plasma; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D029968:Escherichia coli Proteins; D020104:Immunophilins; D037181:Lectins, C-Type; D008565:Membrane Proteins; D018832:Molecular Chaperones; D019696:Peptidylprolyl Isomerase; D019704:Protein Disulfide-Isomerases; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D012177:Retinol-Binding Proteins; D054839:Retinol-Binding Proteins, Plasma; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C490900:SurA protein, E coli; C068254:FkpA protein, E coli