[Cloning of major outer membrane protein gene of Legionella pneumophila and detection of its expression in prokaryotic cell].
In this study, the ompS gene, a major outer membrane protein gene of Legionella pneumophila, was obtained from the DNA of Legionella pneumophila by PCR. The gene was cloned into prokaryotic expressional plasmid pUC18 to construct recombinant plasmid. The recombinant plasmid was transformed into E. coli strain BL21. The identification was made by means of restriction enzyme analysis, polymerase chain reaction, DNA sequencing analysis, SDS--polyacrylamine gel electrophoresis analysis and Western blot. The results showed that the ompS gene of 914 bp was amplified from Legionella pneumophila DNA, the recombinant plasmid pLPompS was constructed and its expression in prokaryotic cell was detected successfully.
Zhang
Lei
L
Department of Parasiotology, West China Medical Center, Sichuan University, Chengdu 610041, China.
Chen
Jianping
J
Wang
Tao
T
Zhang
Li
L
Tian
Yu
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D016952:Legionella pneumophila / Q000235:genetics*; D008969:Molecular Sequence Data; D018272:Porins / Q000096:biosynthesis* / Q000235:genetics; D011387:Prokaryotic Cells / Q000378:metabolism*
D001426:Bacterial Proteins; D018272:Porins; C072453:major outer membrane protein, Legionella pneumophila