title: Isolation and characterization of UHMWPE wear particles down to ten nanometers in size from in vitro hip and knee joint simulators.
abstract: There is currently considerable interest in the wear debris and osteolytic potential of different types of bearings used in total joint replacements. The biological activity of the wear debris is dependent on the size and volume of the particles produced. Wear volume also plays an important role in the functional biological activity of a joint replacement. In vitro studies have shown that crosslinking of ultra high molecular weight polyethylene (UHMWPE) acetabular cups and tibial trays produces a reduction in wear volume, and crosslinking has now been introduced clinically for both types of prostheses. Previous studies have identified both micron and submicron-sized polyethylene wear particles. The aim of this study was to characterize the wear and wear particles generated from moderately crosslinked GUR 1,020 GVF UHMWPE acetabular cups and tibial trays in hip and knee joint wear simulators down to 10 nanometers in size. The wear rates of the two prosthesis types were very similar at 25.6 +/- 5.3 mm(3) per million cycles for the hip prostheses and 22.75 +/- 5.95 mm(3) per million cycles for the knee prostheses. Nanometer-sized wear particles were isolated and characterized from both hip and knee simulator lubricants for the first time. Significantly higher numbers (p < 0.05) of particles in the nanometer (<0.1 microm) size range were produced by the hip prostheses compared to the knee prostheses. The knee prostheses produced larger particles, with the mode of particle size in the 0.1-1.0 microm size range, compared to <0.1 microm size range for the hip prostheses. In addition, the knee prostheses produced a greater volumetric concentration of wear particles in the 1.0-10 microm size range, and consequently lower specific biological activity and functional biological activity indices. These results indicated that the knee prostheses had a lower osteolytic potential compared to the hip prostheses.
authors.affiliation: Institute of Medical and Biological Engineering, University of Leeds, Leeds, United Kingdom. j.l.tipper@leeds.ac.uk
authors.forename: J L
authors.initials: JL
authors.lastname: Tipper
authors.forename: A L
authors.initials: AL
authors.lastname: Galvin
authors.forename: S
authors.initials: S
authors.lastname: Williams
authors.forename: H M J
authors.initials: HM
authors.lastname: McEwen
authors.forename: M H
authors.initials: MH
authors.lastname: Stone
authors.forename: E
authors.initials: E
authors.lastname: Ingham
authors.forename: J
authors.initials: J
authors.lastname: Fisher
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000302:isolation & purification; D006622:Hip Prosthesis; D007720:Knee Prosthesis; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D011095:Polyethylenes / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D001672:Biocompatible Materials; D011095:Polyethylenes; C111601:ultra-high molecular weight polyethylene