title: Analysis of gene-expression profiles by oligonucleotide microarray in children with influenza.
abstract: In order to clarify the mechanism of the host response to influenza virus, gene-expression profiles of peripheral blood obtained from paediatric patients with influenza were investigated by oligonucleotide microarray. In the acute phase of influenza, 200 genes were upregulated and 20 genes were downregulated compared with their expression in the convalescent phase. Interferon-regulated genes, such as interferon-induced protein with tetratricopeptide repeats 2 (IFIT2) and vipirin, were strongly upregulated in the acute phase. Gene ontology analysis showed that immune response genes were highly overrepresented among the upregulated genes. Gene-expression profiles of influenza patients with and without febrile convulsion were also studied. In patients with febrile convulsion, 22 genes were upregulated and five were downregulated compared with their expression in patients without febrile convulsion. These results should help to clarify the pathogenesis of influenza and its neurological complications.
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Jun-Ichi
authors.initials: JI
authors.lastname: Kawada
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Kamachi
authors.affiliation: Department of Pediatrics, Nagoya Ekisaikai Hospital, Nagoya, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nishikawa
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Taniguchi
authors.affiliation: Development Center for Targeted and Minimally Invasive Diagnosis and Treatment, Fujita Health University, Toyoake, Japan.|Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan.
authors.forename: Kayuri
authors.initials: K
authors.lastname: Nagaoka
authors.affiliation: Development Center for Targeted and Minimally Invasive Diagnosis and Treatment, Fujita Health University, Toyoake, Japan.|Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kurahashi
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Kojima
authors.affiliation: Department of Pediatrics, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Morishima
mesh_terms: D000208:Acute Disease; D002675:Child, Preschool; D003289:Convalescence; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007223:Infant; D053122:Influenza A Virus, H3N2 Subtype / Q000472:pathogenicity*; D007251:Influenza, Human / Q000150:complications / Q000503:physiopathology* / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D003294:Seizures, Febrile / Q000503:physiopathology / Q000821:virology
chemical_list: D011506:Proteins