[Dependence of Ca2+ release from intracellular stores on NADH and FAD levels in fertilized and unfertilized bovine oocytes].
Relation between NADH and FAD concentrations and the quantity of calcium released from intracellular stores in fertilized and unfertilized bovine oocytes was investigated using luminescent analysis. Inhibition of Ca2+ exit from intracellular stores was detected in degenerative oocytes at metaphase II and 2-cell embryos. The intensity of both NADH and FAD fluorescence increased in 2-cell degenerated embryos, whereas the increase in only NADH fluorescence intensity occurred in degenerated oocytes at metaphase II stage. Degeneration exerted no influence on NADH fluorescence intensity or Ca2+ exit from intracellular stores, whereas a decreased FAD fluorescence intensity was noted in degenerated pronuclei. The obtained data testify that in degenerated zygotes and early embryos Ca2+ release may occur from different intracellular stores.
Denisenko
V Iu
VIu
Kuz'mina
T I
TI
Shokin
O V
OV
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002751:Chlortetracycline; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D066298:In Vitro Techniques; D009243:NAD / Q000378:metabolism*; D009865:Oocytes / Q000201:enzymology / Q000378:metabolism / Q000502:physiology
D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D002118:Calcium; D002751:Chlortetracycline