title: Purification, and properties of a bovine uricase.
abstract: Uricase from bovine kidney, purified to homogeneity level, had a molecular weight of 70 kDa. The apparent K(m) and V(max) values for uric acid hydrolysis were 0.125 mM and 102 IU mg(-1) protein respectively. The activation energy requirement for uric acid hydrolysis by uricase and inactivation of enzyme were 11.6 and 14.5 kJ/M respectively. Both enthalpy (Delta H*) and entropy of activation (Delta S*) for uricase activity were lower than those reported for some thermostable enzymes.
authors.affiliation: National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. mirajoka@nibge.org
authors.forename: Muhammad Ibrahim
authors.initials: MI
authors.lastname: Rajoka
authors.forename: Khalil-Ur-
authors.initials: KU
authors.lastname: Rehman
authors.forename: Munazza
authors.initials: M
authors.lastname: Mehraj
authors.forename: Muhammad Waheed
authors.initials: MW
authors.lastname: Akhtar
authors.forename: Muhammad Anjum
authors.initials: MA
authors.lastname: Zia
mesh_terms: D000818:Animals; D002417:Cattle; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008970:Molecular Weight; D013696:Temperature; D013816:Thermodynamics; D014503:Urate Oxidase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D014503:Urate Oxidase