title: Efficiency of utilizing ileal digestible lysine and threonine for whole body protein deposition in growing pigs is reduced when dietary casein is replaced by wheat shorts.
abstract: To determine the effect of dietary inclusion level of wheat shorts (WS; a high nonstarch poly-saccharide-containing feed ingredient) and casein (CS; a control) on the efficiency of utilizing ileal digestible Lys (kLys) and Thr (kThr) for whole body protein deposition (PD) in the growing pig, 2 separate N-balance studies were conducted with either Lys or Thr as first-limiting AA in cornstarch-based diets. For the Lys study, a basal diet (L-basal) was formulated to contain 0.24 g of standardized ileal digestible (SID) Lys per MJ of DE, to which 0.095 or 0.19 g of SID Lys per MJ of DE were added using either CS (L-CS2 or L-CS3, respectively) or WS (L-WS2 or L-WS3, respectively). A sixth diet was evaluated that was similar to L-CS3 but to which 6% pectin (L-pectin) was added as a source of soluble nonstarch polysaccharides. For the Thr study, the basal diet (T-basal) was formulated to contain 0.14 g of SID Thr per MJ of DE, to which 0.055 or 0.11 g of SID Thr per MJ of DE were added from CS (T-CS2 or T-CS3, respectively) or from WS (T-WS2 and T-WS3, respectively). A sixth diet was evaluated that was similar to T-CS3 but to which 6% pectin was added (T-pectin). Increasing SID Lys intake from CS did not influence kLys for PD (P > 0.10), whereas increasing SID Lys intake from WS reduced kLys for PD (P = 0.001; 89 vs. 79%). Inclusion of 6% pectin had no effect on kLys for PD (P > 0.10). Increasing SID Thr intake from CS also did not influence kThr for PD (P > 0.10), whereas kThr for PD was reduced at the greatest dietary inclusion level of WS (P < 0.001; 90 vs. 77%). Pectin inclusion had no effect on kThr for PD (P > 0.10). The inefficiency of utilizing ileal digestible Lys intake for PD may be attributed to nonreactive Lys in WS. The negative impact of including high levels of WS in the diet of pigs on kThr seems to be associated with fiber content of WS; it was not related to increased endogenous ileal AA losses at the distal ileum. The impact of dietary AA source on the use of ileal digestible Lys and Thr for PD, or other body functions, is substantial and should be considered in the formulation of pig diets. Further research is warranted to elucidate the mechanisms contributing to substantial dietary effects on Thr use for PD.
authors.affiliation: Department of Animal and Poultry Science, University of Guelph, Guelph, ON, N1G 2W1, Canada.
authors.forename: A J
authors.initials: AJ
authors.lastname: Libao-Mercado
authors.forename: S
authors.initials: S
authors.lastname: Leeson
authors.forename: S
authors.initials: S
authors.lastname: Langer
authors.forename: B J
authors.initials: BJ
authors.lastname: Marty
authors.forename: C F M
authors.initials: CF
authors.lastname: de Lange
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002364:Caseins / Q000494:pharmacology*; D004032:Diet; D004063:Digestion; D007082:Ileum / Q000378:metabolism; D008239:Lysine / Q000378:metabolism*; D008297:Male; D011506:Proteins / Q000378:metabolism*; D013552:Swine / Q000254:growth & development* / Q000378:metabolism; D013912:Threonine / Q000378:metabolism*; D014908:Triticum / Q000737:chemistry*
chemical_list: D002364:Caseins; D011506:Proteins; D013912:Threonine; D008239:Lysine