Histamine modulates mast cell degranulation through an indirect mechanism in a model IgE-mediated reaction.
Histamine is released in inflammatory reactions and exerts an immunoregulatory function on cells present in the microenvironment. In this study, we compared the effect of histamine on degranulation of mast cells derived from animals bearing a parasitic infection with those from uninfected animals. Peritoneal mast cells (PMC) were obtained 24 days after infection of Wistar rats with Toxocara canis. The degree of degranulation was assessed either morphologically or by measuring the release of beta-hexosaminidase and TNF-alpha. Non-purified PMC or mast cells immunomagnetically purified with mAb AA4 were used. An increase in degranulation of non-purified mast cells from infected animals was observed after incubation with histamine in vitro or when histamine was injected into the peritoneal cavity. When a purified mast cell population was used, this effect was no longer observed. Supernatants from spleen cells stimulated with histamine induced degranulation of purified mast cells, and again, this was potentiated with PMC from infected animals. However, when supernatants from peritoneal macrophages similarly stimulated were used, a reduction in the degranulation of PMC from infected animals was observed. Our results suggest that histamine may act as a regulator of mast cell degranulation, thus modulating inflammatory responses due to infection with certain parasites.
Carlos
Daniela
D
Universidade de São Paulo (USP), Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Brasil.
Sá-Nunes
Anderson
A
de Paula
Lúcia
L
Matias-Peres
Camila
C
Jamur
Maria Célia
MC
Oliver
Constance
C
Serra
Magda Fraguas
MF
Martins
Marco Aurélio
MA
Faccioli
Lúcia Helena
LH
D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects / Q000276:immunology*; D002927:Cimetidine / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006632:Histamine / Q000276:immunology* / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007150:Immunohistochemistry; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008407:Mast Cells / Q000166:cytology / Q000276:immunology / Q000469:parasitology / Q000502:physiology*; D009569:Nitric Oxide / Q000096:biosynthesis / Q000276:immunology; D010529:Peritoneal Cavity / Q000166:cytology; D011738:Pyrilamine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017166:Toxocara canis / Q000276:immunology*; D014120:Toxocariasis / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D001619:beta-N-Acetylhexosaminidases / Q000276:immunology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D002927:Cimetidine; D006632:Histamine; D001619:beta-N-Acetylhexosaminidases; D011738:Pyrilamine