Phytase production by thermophilic mold Sporotrichum thermophile in solid-state fermentation and its application in dephytinization of sesame oil cake.
The phytase production by Sporotrichum thermophile TLR50 was recorded on all the commonly used animal feed ingredients tested to varying degrees in solid-state fermentation. Enzyme production increased to 180 U/g of dry moldy residue (DMR) in sesame oil cake at 120 h and 45 degrees C at the initial substrate-to-moisture ratio of 1:2.5 and aw of 0.95. Supplementation of sesame oil cake with glucose and ammonium sulfate further enhanced phytase titer (282 U/g of DMR). An overall 76% enhancement in phytase production was achieved owing to optimization. The mold secreted acid phosphatase, amylase, xylanase, and lipase along with phytase. By the action of phytase, inorganic phosphate was liberated efficiently, leading to dephytinization of sesame oil cake.
Singh
Bijender
B
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi-110 021, India.
Satyanarayana
T
T
D010832:6-Phytase / Q000096:biosynthesis* / Q000302:isolation & purification; D000135:Acid Phosphatase / Q000096:biosynthesis; D000645:Ammonium Sulfate / Q000494:pharmacology; D000681:Amylases / Q000096:biosynthesis; D000821:Animal Feed; D000818:Animals; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis; D004789:Enzyme Activation; D005285:Fermentation; D005947:Glucose / Q000494:pharmacology; D007218:Industrial Microbiology; D008049:Lipase / Q000096:biosynthesis; D010833:Phytic Acid / Q000737:chemistry / Q000378:metabolism*; D012715:Sesame Oil / Q000378:metabolism*; D013175:Sporothrix / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors
D010833:Phytic Acid; D012715:Sesame Oil; D008049:Lipase; D000135:Acid Phosphatase; D010832:6-Phytase; D000681:Amylases; D043364:Endo-1,4-beta Xylanases; D005947:Glucose; D000645:Ammonium Sulfate