title: The relationship of the expression of estrogen receptor in cartilage cell and osteoarthritis induced by bilateral ovariectomy in guinea pig.
abstract: To investigate the estrogen receptor (ER) expression in cartilage cell in the development of osteoarthritis induced by bilateral ovariectomy in guinea pig and to find their relationship. 30 two-month-old female guinea pigs were randomly divided into two groups (n = 15 each): sham operation (control) group and ovariectomized group (OVX); Scanning electorne microscope (SEM) and transmission electron microscope (TEM) were obtained to analysis the cartilage degeneration of the hind limb knee joint after 6 and 12 weeks of ovariectomy. Dextran-Coated-Charcoal (DCC) was taken to quantitively detect the expression of ER. The serum levels of estrogen and gestone were detected by immune contest assay. The results showed that ER do exist in the cartilages of the guinea pigs, with higher expression in the control group than in OVX group at the same time point (P < 0.05). It was increased also at 12 th week after operation than that of preoperation. The blood serum levels of estrogen and gestone showed a similar tendency to the expression of ER. Joint cartilage degeneration detected by SEM and TEM could be found at 6 th week, but severe degenerative lesions at 12 th week in the OVX group compared with the control group (P < 0.01). The data suggested that bilateral ovariectomy in guinea pig lead to severe osteoarthritis which mighgt be related to the lower serum level of estrogen and the downregulation of the expression of ER in the cartilage also.
authors.affiliation: Department of Orthopaedics, Oilu Hospital Affiliated Shan Dong University, Jinan 250012, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Dai
authors.forename: Jianmin
authors.initials: J
authors.lastname: Li
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.forename: Qiaohui
authors.initials: Q
authors.lastname: Liu
authors.forename: Chunmei
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D019902:Chondrocytes / Q000378:metabolism; D004967:Estrogens / Q000097:blood*; D005260:Female; D006168:Guinea Pigs; D010003:Osteoarthritis / Q000209:etiology* / Q000378:metabolism; D010052:Ovariectomy; D011897:Random Allocation; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004967:Estrogens; D011960:Receptors, Estrogen