title: [Quantitative study of osteoclastic related factors in the process of bone reconstruction].
abstract: OBJECTIVE
  To detect the expression of osteoclast related factors, tumor necrosis factor-alpha (TNF-alpha), receptor activator of NF-kappaB ligand (RANKL) and tartrate-resistant acid phosphatase (TRAP), in the process of bone remodeling.
  
  
  METHODS
  8-week-old male C57BL/6J mice were employed in this study to detect the expression of osteoclast related factors by real time PCR.
  
  
  RESULTS
  TNF-alpha, RANKL and TRAP were up regulated in the process of bone remodeling, they reached the peak on day 2, 5 and 10 individually after injury.
  
  
  CONCLUSIONS
  Osteoclast related factors also participate in bone remodeling, which depends on the delicate balance between bone formation and bone resorption.
authors.affiliation: Dept. of Oral Science, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chang
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Hou
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Sibata
authors.forename: Tomoo
authors.initials: T
authors.lastname: Tsukazaki
authors.forename: Akira
authors.initials: A
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D001862:Bone Resorption; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B; D010010:Osteoclasts; D010012:Osteogenesis; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D014409:Tumor Necrosis Factor-alpha
chemical_list: D016328:NF-kappa B; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D014409:Tumor Necrosis Factor-alpha