title: Correlation between pharmacological efficacy of cyclosporine A and tacrolimus, evaluated by lymphocyte immunosuppressant-sensitivity test (LIST) with MTT assay procedure in renal transplant recipients.
abstract: The dose of calcineurin inhibitors in renal transplantation has been adjusted, based on the therapeutic drug monitoring data. However, the data do not always correlate with clinical drug efficacy. In vitro response of peripheral-blood mononuclear cells to immunosuppressive drugs is reported to correlate with the recipient-response to therapeutic efficacy of the drug. We report, here, usefulness of a lymphocyte immunosuppressant sensitivity test for the estimation of individual drug sensitivity in renal transplant recipients. The LIST we have developed includes MTT assay procedures without the use of radioisotope-labeled compounds, which is convenient for general hospital use. Utilizing this procedure, we compared the pharmacological efficacy between cyclosporine A and tacrolimus in 41 renal transplant recipients.
authors.affiliation: Division of Pharmacy, Niigata University Medical and Dental Hospital, Niigata City, Niigata, Japan. sugiyama-nii@umin.ac.jp
authors.forename: Kentaro
authors.initials: K
authors.lastname: Sugiyama
authors.forename: Keiko
authors.initials: K
authors.lastname: Arakawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Satoh
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Saito
authors.forename: Kota
authors.initials: K
authors.lastname: Takahashi
authors.forename: Noriko
authors.initials: N
authors.lastname: Saito
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Hirano
mesh_terms: D000328:Adult; D065095:Calcineurin Inhibitors; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006084:Graft Rejection / Q000517:prevention & control; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000494:pharmacology*; D016030:Kidney Transplantation / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016559:Tacrolimus / Q000008:administration & dosage / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D065095:Calcineurin Inhibitors; D007166:Immunosuppressive Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D016572:Cyclosporine; C022616:thiazolyl blue; D016559:Tacrolimus