title: Collagen I-coated titanium surfaces: mesenchymal cell adhesion and in vivo evaluation in trabecular bone implants.
abstract: The goal of the study was the evaluation of the effect of modification of titanium implants by acrylic acid surface grafting-collagen I coupling. Tests were performed on titanium samples treated by galvanostatic anodization to create a porous surface topography. Surface characterization by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) confirms the biochemical modification of the surface and shows a surface topography characterized by pores mostly below 1 mum diameter. In vitro evaluation involving human mesenchymal cells shows enhanced cell growth on collagen coated surfaces as compared to titanium ones. Four weeks in vivo evaluation of implants in rabbit femur trabecular bone shows improvements of bone-to-implant contact, while improvement of bone ingrowth is slightly not significant (p = 0.056), when compared to the control. Overall, these data indicate that integration in trabecular, or cancellous, bone can be enhanced by the surface collagen layer, confirming previous findings obtained by modification of machined surfaces by the same approach in cortical bone implants.
authors.affiliation: Nobil Bio Ricerche, Villafranca d'Asti, Italy. mmorra@nobilbio.it
authors.forename: M
authors.initials: M
authors.lastname: Morra
authors.forename: C
authors.initials: C
authors.lastname: Cassinelli
authors.forename: G
authors.initials: G
authors.lastname: Cascardo
authors.forename: L
authors.initials: L
authors.lastname: Mazzucco
authors.forename: P
authors.initials: P
authors.lastname: Borzini
authors.forename: M
authors.initials: M
authors.lastname: Fini
authors.forename: G
authors.initials: G
authors.lastname: Giavaresi
authors.forename: R
authors.initials: R
authors.lastname: Giardino
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D018786:Bone Substitutes; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D006801:Humans; D008648:Mesoderm / Q000166:cytology / Q000502:physiology*; D019736:Prostheses and Implants; D011817:Rabbits; D014025:Titanium
chemical_list: D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D014025:Titanium