title: Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine.
abstract: A thermophilic facultative bacterial isolate was recovered from 3.2km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 degrees C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 degrees C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O(2), GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6microm longx0.5microm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366(T) (99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans.
authors.affiliation: Geosyntec Consultants, 3131 Princeton Pike, Building 1B, Suite 205, Lawrenceville, NJ 08648, USA. mdeflaun@geosyntec.com
authors.forename: M F
authors.initials: MF
authors.lastname: Deflaun
authors.forename: J K
authors.initials: JK
authors.lastname: Fredrickson
authors.forename: H
authors.initials: H
authors.lastname: Dong
authors.forename: S M
authors.initials: SM
authors.lastname: Pfiffner
authors.forename: T C
authors.initials: TC
authors.lastname: Onstott
authors.forename: D L
authors.initials: DL
authors.lastname: Balkwill
authors.forename: S H
authors.initials: SH
authors.lastname: Streger
authors.forename: E
authors.initials: E
authors.lastname: Stackebrandt
authors.forename: S
authors.initials: S
authors.lastname: Knoessen
authors.forename: E
authors.initials: E
authors.lastname: van Heerden
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D002475:Cellobiose / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005407:Flagella; D020459:Genes, rRNA; D006046:Gold; D006358:Hot Temperature; D006838:Hydrocarbons / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008906:Mining; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013019:South Africa; D013171:Spores, Bacterial
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006838:Hydrocarbons; D009566:Nitrates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002475:Cellobiose; D019344:Lactic Acid; D006046:Gold