Cloning and characterization of the dromedary (Camelus dromedarius) neonatal Fc receptor (drFcRn).
The full length cDNA of the dromedary neonatal Fc receptor (drFcRn) alpha chain was isolated and found that it is similar to the neonatal Fc receptor (FcRn) of other species with a high overall similarity to ruminant FcRn alpha chains. The drFcRn/Fc contact residues are highly conserved and predicted to bind both conventional (IgG1) and heavy chain (IgG2a, IgG3) antibodies. Using immunohistochemistry, we detected its expression in the hepatocytes and in epithelial cells of portal bile ductuli and also in the mammary gland acini and ducti. Remarkably, Ser313, that was identified to be crucial for apical to basolateral transcytosis, is substituted in the drFcRn alpha chain. The full length of the dog and orangutan FcRn alpha chains was also identified from databases. Analyzing the phylogenetic relatedness of this gene we found that dromedary clustered together with artiodactyls, dog is located between artiodactyls and primates, where the orangutan was branched, reflecting the accepted evolutionary relationships.
Kacskovics
Imre
I
Department of Physiology and Biochemistry, Faculty of Veterinary Science, Szent István University, Istvan utca 2. H-1078, Budapest, Hungary. kacskovics.imre@aotk.szie.hu
Mayer
Balázs
B
Kis
Zsuzsanna
Z
Frenyó
László V
LV
Zhao
Yaofeng
Y
Muyldermans
Serge
S
Hammarström
Lennart
L
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002162:Camelus / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D007137:Immunoglobulin alpha-Chains / Q000235:genetics / Q000276:immunology; D007150:Immunohistochemistry / Q000662:veterinary; D008099:Liver / Q000276:immunology; D008321:Mammary Glands, Animal / Q000276:immunology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011961:Receptors, Fc / Q000235:genetics* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
D018076:DNA, Complementary; D015395:Histocompatibility Antigens Class I; D007137:Immunoglobulin alpha-Chains; D011961:Receptors, Fc; D012313:RNA; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase; C100688:Fc receptor, neonatal