Anti-inflammatory effect of jeongshintang through suppression of p38 activation in human astrocytoma, U373MG cells.
Jeongshintang (JST) is a Korean herbal prescription, which has been successfully used for cerebral diseases. However, the anti-inflammatory effect of JST on Alzheimer's disease (AD) is still not fully understood. In this study, we investigated the effects of JST in attenuating the inflammatory response induced by interleukin (IL)-1beta plus beta-amyloid [1-42] fragment (A beta) in the human astrocyte cell line, U373MG. The production of IL-6, IL-8, and prostaglandin (PG)E2 was significantly increased by IL-1beta plus A beta (1-42) in a time-dependent manner (P < 0.05). JST significantly inhibited the IL-1beta plus A beta (1-42)-induced IL-6, IL-8, and PGE2 production at 24 h (P < 0.05). Maximal inhibition rate of IL-6, IL-8, and PGE2 production by JST was about 54.40%, 56.01%, and 44.06% respectively. JST (0.01-1 mg/ml) also attenuated the expression of cyclooxygenase (COX)-2 and activation of p38 MAPK induced by IL-1beta and A beta (1-42). These results demonstrated that JST has an anti-inflammatory effect, which might explain its beneficial effect in the treatment of various neurodegenerative diseases such as AD.
Kim
S J
SJ
College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul 130-701, Republic of Korea.
Jeong
H J
HJ
Kim
B K
BK
Kim
N H
NH
Kim
J S
JS
Choi
K S
KS
Lee
H J
HJ
Kang
S T
ST
Shin
S S
SS
Kim
W I
WI
Eom
H S
HS
Lee
K M
KM
Um
J Y
JY
Hong
S H
SH
Kim
H M
HM
D016229:Amyloid beta-Peptides / Q000633:toxicity; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001254:Astrocytoma; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2; D015232:Dinoprostone / Q000037:antagonists & inhibitors; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000633:toxicity; D016209:Interleukin-8 / Q000037:antagonists & inhibitors; D008565:Membrane Proteins / Q000037:antagonists & inhibitors; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010446:Peptide Fragments / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D000093242:Interleukin-6 Inhibitors
D016229:Amyloid beta-Peptides; D000893:Anti-Inflammatory Agents; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D007375:Interleukin-1; D016209:Interleukin-8; D008565:Membrane Proteins; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D010936:Plant Extracts; D047428:Protein Kinase Inhibitors; D048051:p38 Mitogen-Activated Protein Kinases; D000093242:Interleukin-6 Inhibitors; C075222:amyloid beta-protein (1-42); C513555:jeongshintang; C496540:PTGS2 protein, human