Serum iohexol analysis by micellar electrokinetic capillary chromatography.
A simple and rapid ( approximately 4 min) method for the measurement of iohexol in serum for assessing the glomerular filtration rate is described. It is based on direct serum injection on the capillary by MEKC. The method is linear between 8 and 260 mg/L, with an RSD of peak height of 2.9%. Several simple steps have contributed to an improved daily precision, such as choosing a high pH buffer, increasing the SDS concentration, frequent standardization, and eliminating any sample pretreatment.
Shihabi
Zak K
ZK
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. zshihabi@wfubmc.edu
Hinsdale
Mark E
ME
D001774:Blood Chemical Analysis / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003287:Contrast Media / Q000032:analysis*; D019075:Electrophoresis, Capillary; D017022:Flow Injection Analysis; D005919:Glomerular Filtration Rate; D006801:Humans; D007472:Iohexol / Q000032:analysis*; D044967:Serum / Q000737:chemistry
D003287:Contrast Media; D007472:Iohexol