title: Effect of recombinant human interleukin-11 on expressions of interleukin-11 receptor alpha-chain and glycoprotein 130 in intestinal epithelium cell line-6 after neutron irradiation.
abstract: OBJECTIVE
  To explore the effect of recombinant human interleukin-11 (rhIL-11) on the expressions of interleukin-11 receptor alpha-chain (IL-11Ralpha) and an additional signal transducer glycoprotein 130 (gp130) in intestinal epithelium cell line-6 (IEC-6) after neutron irradiation.
  
  
  METHODS
  Cultured IEC-6 cells were exposed to 4.0Gy neutron and treated with 100 ng/mL rhIL-11 12 h prior to or immediately after irradiation. The apoptosis and necrosis rates and expressions of IL-11Ralpha and gp130 were observed by flow cytometry, immunohistochemistry, Western blot and image analysis.
  
  
  RESULTS
  The apoptosis rate of IEC-6 cells was increased by irradiation at 6 h (P < 0.01), IL-11 stimulation resulted in a decreased apoptosis rate in irradiated IEC-6 cells (P < 0.05). In normal control IEC-6 cells, intense immunoreactivity of IL-11Ralpha was located within the cell membrane and cytoplasm. The level of IL-11Ralpha expression significantly decreased at 6 h after irradiation (P < 0.01) and restored at 24 h after irradiation. In IEC-6 cells treated with both radiation and rhIL-11, the level of IL-11Ralpha expression was higher than that of irradiated cells (P < 0.05). When it came to gp130 protein, it was located in the cytoplasm of IEC-6 cells. After irradiation, we found a progressive decrease in the expression of gp130 protein (P < 0.05) in 48 h post-radiation, while in rhIL-11-stimulated cells, it came back to normal level at 24 h after irradiation and decreased at 48 h, but was still higher than that of only irradiated cells (P < 0.05).
  
  
  CONCLUSIONS
  rhIL-11 can protect IEC-6 cells from neutron irradiation. The protective effect of rhIL-11 might be connected with its ability to up-regulate the expressions of specific ligand-binding subunit IL-11Ralpha and signal-transducing subunit gp130.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Rui-Juan
authors.initials: RJ
authors.lastname: Wang
authors.forename: Rui-Yun
authors.initials: RY
authors.lastname: Peng
authors.forename: Kai-Fei
authors.initials: KF
authors.lastname: Fu
authors.forename: Ya-Bing
authors.initials: YB
authors.lastname: Gao
authors.forename: Rui-Gang
authors.initials: RG
authors.lastname: Han
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Hu
authors.forename: Qing-Liang
authors.initials: QL
authors.lastname: Luo
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Ma
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D015153:Blotting, Western; D002460:Cell Line; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D017370:Interleukin-11 / Q000032:analysis / Q000235:genetics / Q000494:pharmacology*; D053703:Interleukin-11 Receptor alpha Subunit; D007413:Intestinal Mucosa / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D009502:Neutrons / Q000009:adverse effects*; D011832:Radiation Injuries / Q000517:prevention & control; D051381:Rats; D018123:Receptors, Interleukin / Q000032:analysis / Q000235:genetics*; D053702:Receptors, Interleukin-11; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects
chemical_list: D006023:Glycoproteins; C508611:IL11 protein, human; C508428:IL11RA protein, human; C508430:Il11ra1 protein, rat; D017370:Interleukin-11; D053703:Interleukin-11 Receptor alpha Subunit; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11; D011994:Recombinant Proteins; C480482:glycoprotein 130, human