Survivin promoter polymorphism and cervical carcinogenesis.
BACKGROUND
Survivin, a novel member of the inhibitor of apoptosis family, plays an important role in cell cycle regulation. A common polymorphism at the survivin gene promoter (G/C at position 31) was shown to be correlated with survivin gene expression in cancer cell lines.


OBJECTIVE
To investigate whether this polymorphism could be involved in the development of human papillomavirus (HPV)-associated cervical carcinoma.


METHODS
Survivin promoter polymorphism was detected in patients with cervical cancer, in patients with equivocal cytological atypia and in a control population using polymerase chain reaction (PCR-restriction fragment length polymorphism (RFLP) and PCR-single strand conformation polymorphism analysis. HPV was typed in patients with cervical cancer and cytological atypia using PCR-RFLP.


RESULTS
No statistically significant differences were found in the genotype distributions of the survivin promoter variants among our study groups.


CONCLUSIONS
The survivin promoter polymorphism at position 31 may not represent an increased risk for the development of cervical cancer, at least in the population studied here.
Borbély
A A
AA
Department of Medical Microbiology, Medical and Health Science Centre, University of Debrecen, Debrecen, Hungary.
Murvai
M
M
Szarka
K
K
Kónya
J
J
Gergely
L
L
Hernádi
Z
Z
Veress
G
G
D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D030361:Papillomavirus Infections / Q000150:complications; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics; D000077022:Survivin; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000821:virology
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D000077022:Survivin