Evidence that a significant number of naive T cells enter non-lymphoid organs as part of a normal migratory pathway.
Only activated and effector memory T cells are thought to access non-lymphoid tissues. In contrast, naive T cells are thought to circulate only between the blood, lymph and secondary lymphoid organs. We examined the phenotype of endogenous T cells in various non-lymphoid organs and showed that a subset of cells exhibited an apparently naive phenotype and were functionally inactive. FTY720 treatment selectively depleted this population from the non-lymphoid tissues. In addition, RAG-deficient TCR transgenic CD4 and CD8 T cells were present in non-lymphoid tissues in bone marrow chimeric mice and in situ imaging analysis revealed their location in the parenchymal tissues. Moreover, migration of TCR transgenic T cells to non-lymphoid tissues after adoptive transfer was pertussis-toxin resistant. Overall, the results suggest that naive T cells may circulate through non-lymphoid tissues as part of their normal migratory pathway.
Cose
Stephen
S
Department of Immunology, University of Connecticut Health Center, Farmington, 06030, USA.
Brammer
Clair
C
Khanna
Kamal M
KM
Masopust
David
D
Lefrançois
Leo
L
D000818:Animals; D001921:Brain / Q000276:immunology; D040881:CD11a Antigen / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D000068876:Fingolimod Hydrochloride; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008099:Liver / Q000276:immunology; D008168:Lung / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D037342:Pertussis Toxin / Q000494:pharmacology; D011409:Propylene Glycols / Q000494:pharmacology; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000276:immunology; D012867:Skin / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D013110:Sphingosine / Q000031:analogs & derivatives / Q000494:pharmacology
D040881:CD11a Antigen; C515371:Ccr7 protein, mouse; D007166:Immunosuppressive Agents; D011409:Propylene Glycols; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D037342:Pertussis Toxin; D000068876:Fingolimod Hydrochloride; D013110:Sphingosine