title: A cost effective fermentative production of succinic acid from cane molasses and corn steep liquor by Escherichia coli.
abstract: OBJECTIVE
  Development and optimization of an efficient and inexpensive medium for succinic acid production by Escherichia coli under anaerobic conditions.
  
  
  RESULTS
  Initially, 0.8 gl(-1) of succinic acid was produced in 60 h in 300-ml medium. On optimization, glucose and peptone were replaced by cane molasses and corn steep liquor. Three hundred ml of this medium was inoculated with 4% (v/v) of seed inoculum, incubated at 39 degrees C for 72 h, resulted in 7.1 gl(-1) of succinic acid in 36 h. Scale up in a 10-l fermentor under conditions of controlled pH and continuous CO2 supply in this medium resulted in 17 gl(-1) of succinic acid in 30 h.
  
  
  CONCLUSIONS
  A ninefold increase in succinic acid production was obtained in 500-ml anaerobic bottles with optimized medium having cane molasses and corn steep liquor as against initial medium containing glucose and peptone. However, a subsequent scale up in a 10-l fermentor resulted in a 2.5-fold increase in succinic acid production as against optimized medium used in 500-ml anaerobic bottles.
  
  
  CONCLUSIONS
  Succinic acid production was enhanced in medium consisting of inexpensive carbon and nitrogen sources in a shorter span of time.
authors.affiliation: Department of Microbiology, University of Delhi, New Delhi, India.
authors.forename: L
authors.initials: L
authors.lastname: Agarwal
authors.forename: J
authors.initials: J
authors.lastname: Isar
authors.forename: G K
authors.initials: GK
authors.lastname: Meghwanshi
authors.forename: R K
authors.initials: RK
authors.lastname: Saxena
mesh_terms: D000818:Animals; D019149:Bioreactors / Q000191:economics*; D002020:Buffaloes; D003362:Cost-Benefit Analysis; D003470:Culture Media; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D012417:Rumen / Q000382:microbiology; D031786:Saccharum; D019802:Succinic Acid / Q000032:analysis / Q000378:metabolism*; D003313:Zea mays
chemical_list: D003470:Culture Media; D019802:Succinic Acid