title: GAS41 interacts with transcription factor AP-2beta and stimulates AP-2beta-mediated transactivation.
abstract: Transcription factor AP-2 regulates transcription of a number of genes involving mammalian development, differentiation and carcinogenesis. Recent studies have shown that interaction partners can modulate the transcriptional activity of AP-2 over the downstream targets. In this study, we reported the identification of GAS41 as an interaction partner of AP-2beta. We documented the interaction both in vivo by co-immunoprecipitation as well as in vitro through glutathione S-transferase (GST) pull-down assays. We also showed that the two proteins are co-localized in the nuclei of mammalian cells. We further mapped the interaction domains between the two proteins to the C-termini of both AP-2beta and GAS41, respectively. Furthermore, we have identified three critical residues of GAS41 that are important for the interaction between the two proteins. In addition, by transient co-expression experiments using reporter containing three AP-2 consensus binding sites in the promoter region, we found that GAS41 stimulates the transcriptional activity of AP-2beta over the reporter. Finally, electrophoretic mobility shift assay (EMSA) suggested that GAS41 enhances the DNA-binding activity of AP-2beta. Our data provide evidence for a novel cellular function of GAS41 as a transcriptional co-activator for AP-2beta.
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Science, Hunan Normal University, Changsha, Hunan 410081, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ding
authors.forename: Changzheng
authors.initials: C
authors.lastname: Fan
authors.forename: Jianlin
authors.initials: J
authors.lastname: Zhou
authors.forename: Yingli
authors.initials: Y
authors.lastname: Zhong
authors.forename: Rushi
authors.initials: R
authors.lastname: Liu
authors.forename: Kaiqun
authors.initials: K
authors.lastname: Ren
authors.forename: Xiang
authors.initials: X
authors.lastname: Hu
authors.forename: Chang
authors.initials: C
authors.lastname: Luo
authors.forename: Shunyong
authors.initials: S
authors.lastname: Xiao
authors.forename: Yeqi
authors.initials: Y
authors.lastname: Wang
authors.forename: Du
authors.initials: D
authors.lastname: Feng
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001665:Binding Sites; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D004247:DNA / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D050656:Transcription Factor AP-2 / Q000032:analysis / Q000378:metabolism*; D014157:Transcription Factors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques
chemical_list: D050656:Transcription Factor AP-2; D014157:Transcription Factors; C109446:YEATS4 protein, human; D004247:DNA