title: Transformation of the entomopathogenic fungus Paecilomyces fumosoroseus with Agrobacterium tumefaciens.
abstract: OBJECTIVE
  To test the suitability of the Agrobacterium tumefaciens-mediated transformation (AMT) method with Paecilomyces fumosoroseus, a fungal pathogen that causes diseases in a wide range of insects including whiteflies.
  
  
  RESULTS
  Conidia of P. fumosoroseus were successfully transformed to hygromycin B resistance using the hph gene of Escherichia coli as the selectable marker. Transformation frequencies were 58.3 +/- 18.5, 98.3 +/- 24.8 and 169.7 +/- 35.5 (+/-SEM) transformants per 10(5), 10(6) and 10(7) target conidia respectively. After confirmation by PCR, transformants were subjected to Southern analysis, and the results revealed that 45% (four of nine) of the transformants contained single-copy integration of the T-DNA.
  
  
  CONCLUSIONS
  In our AMT system, we efficiently transformed conidia of P. fumosoroseus. The employment of this method circumvents time-consuming protoplast preparation and allows the isolation of transformants containing single-copy integration of the T-DNA.
  
  
  CONCLUSIONS
  Considering the efficiency of Ag. tumefaciens-mediated transformation, this method represents a useful tool for insertional mutagenesis to characterize genes that are important for the pathogenicity of P. fumosoroseus.
authors.affiliation: Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina-PR, Brazil.
authors.forename: I G P
authors.initials: IG
authors.lastname: Lima
authors.forename: R T D
authors.initials: RT
authors.lastname: Duarte
authors.forename: L
authors.initials: L
authors.lastname: Furlaneto
authors.forename: C H
authors.initials: CH
authors.lastname: Baroni
authors.forename: M H P
authors.initials: MH
authors.lastname: Fungaro
authors.forename: M C
authors.initials: MC
authors.lastname: Furlaneto
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000818:Animals; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006921:Hygromycin B / Q000494:pharmacology; D007313:Insecta / Q000382:microbiology*; D010143:Paecilomyces / Q000235:genetics* / Q000472:pathogenicity*; D014170:Transformation, Genetic
chemical_list: D006921:Hygromycin B