title: Precision study on capillary electrophoresis methods for metacycline.
abstract: A CE method for metacycline (MTC) determination was investigated in an inter-laboratory experiment. Many problems were encountered in this study, most of which were related to the transfer of the method to different CE equipment. The reported problems could be classified into different categories: problems related to the precision, to the parameters in the protocol, and to the MTC peak shape. As the peak shape problem was partially responsible for the poor precision, a new CE method was developed in order to obtain a good MTC peak shape on all equipment. The precision of this new method for MTC determination was examined in an intermediate precision study, where the influence of the factors "time" and "equipment" was investigated. Although the new method could be transferred to different instruments, the precision remained poor mainly due to the contributions of the between-replicate and the between-injection variances.
authors.affiliation: Laboratory of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Thao Do
authors.initials: TD
authors.lastname: Thi
authors.forename: Romeo
authors.initials: R
authors.lastname: Pomponio
authors.forename: Roberto
authors.initials: R
authors.lastname: Gotti
authors.forename: Jan
authors.initials: J
authors.lastname: Saevels
authors.forename: Ben
authors.initials: B
authors.lastname: Van Hove
authors.forename: Willy
authors.initials: W
authors.lastname: Van Ael
authors.forename: Nele
authors.initials: N
authors.lastname: Matthijs
authors.forename: Yvan
authors.initials: Y
authors.lastname: Vander Heyden
authors.forename: Roland Marini
authors.initials: RM
authors.lastname: Djan'geing'a
authors.forename: Patrice
authors.initials: P
authors.lastname: Chiap
authors.forename: Philippe
authors.initials: P
authors.lastname: Hubert
authors.forename: Jacques
authors.initials: J
authors.lastname: Crommen
authors.forename: Huguette
authors.initials: H
authors.lastname: Fabre
authors.forename: Pieter
authors.initials: P
authors.lastname: Dehouck
authors.forename: Jos
authors.initials: J
authors.lastname: Hoogmartens
authors.forename: Ann
authors.initials: A
authors.lastname: Van Schepdael
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008690:Methacycline / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D008690:Methacycline