title: Changes of expression of stretch-activated potassium channel TREK-1 mRNA and protein in hypertrophic myocardium.
abstract: The expression of stretch-activated potassium channel TREK-1 mRNA and protein of hypertrophic myocardium was measured. Using a model of hypertrophy induced by coarctation of abdominal aorta in male Wistar rats, the expression of TREK-1 mRNA and protein was detected by using semi-quantitative RT PCR and Western blot respectively. At 4th and 8th week after constriction of the abdominal aorta, rats developed significant left ventricular hypertrophy. As compared to sham-operated group, stretch-activated potassium channel TREK-1 mRNA was strongly expressed and protein was up-regulated in operation groups (P < 0.05). It was concluded that the expression of TREK-1 was up-regulated in hypertrophic myocardium induced by chronic pressure overload in Wistar rats.
authors.affiliation: Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Longxian
authors.initials: L
authors.lastname: Cheng
authors.forename: Fengcheng
authors.initials: F
authors.lastname: Su
authors.forename: Nsenga
authors.initials: N
authors.lastname: Ripen
authors.forename: Hong
authors.initials: H
authors.lastname: Fan
authors.forename: Kai
authors.initials: K
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Hongyu
authors.initials: H
authors.lastname: Peng
authors.forename: Chunli
authors.initials: C
authors.lastname: Mei
authors.forename: Fang
authors.initials: F
authors.lastname: Zhao
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Liao
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal; D003250:Constriction; D006984:Hypertrophy; D017379:Hypertrophy, Left Ventricular / Q000209:etiology / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D024683:Potassium Channels, Tandem Pore Domain / Q000096:biosynthesis* / Q000235:genetics; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation; D020257:Ventricular Remodeling
chemical_list: D024683:Potassium Channels, Tandem Pore Domain; D011506:Proteins; D012333:RNA, Messenger; C103742:potassium channel protein TREK-1