title: Integron presence in a multiresistant Morganella morganii isolate.
abstract: A multiresistant strain of Morganella morganii was isolated from a patient affected by several severe pathologies. The isolate was found to be resistant to the following antimicrobials: ampicillin, nalidixic acid, cefalothin, cefoxitin, ceftriaxone, ciprofloxacin, chloramphenicol, streptomycin, erythromycin, gentamicin, novobiocin, penicillin, rifampicin, tetracycline and violet crystal. Mechanisms leading to this multiresistance were studied. Porins of M. morganii multiresistant and wild-type strains were analysed by sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and were characterised by their ability to form channels in planar black lipid bilayers. The channels formed by porins from multiresistant and susceptible strains suggested that the porins of the multiresistant strain were not responsible for resistance. A 6.6 kb plasmid (pML2003) was detected, isolated and studied. pML2003 included two integrons. Direct sequencing revealed that one of the integrons contained two cassettes, aminoglycoside adenyltransferase (aadB) and chloramphenicol acetyltransferase (catB3) conferring resistance to aminoglycosides and chloramphenicol, respectively. The second integron contained carbenicillinase (blaP1b) and adenyltransferase (aadA2), which confer resistance to beta-lactamases and streptomycin, respectively.
authors.affiliation: Section of Microbiology, Department of Pathology and Experimental Therapeutics, Campus de Bellvitge, University of Barcelona, Barcelona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Rojas
authors.forename: Teresa
authors.initials: T
authors.lastname: Vinuesa
authors.forename: Fe
authors.initials: F
authors.lastname: Tubau
authors.forename: Consol
authors.initials: C
authors.lastname: Truchero
authors.forename: Roland
authors.initials: R
authors.lastname: Benz
authors.forename: Miguel
authors.initials: M
authors.lastname: Viñas
mesh_terms: D024901:Drug Resistance, Multiple, Bacterial; D032023:Integrons; D008826:Microbial Sensitivity Tests; D020613:Morganella morganii / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D010957:Plasmids; D018272:Porins / Q000302:isolation & purification / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology
chemical_list: D018272:Porins; D011189:Potassium Chloride