Identification of two novel HLA-C alleles, Cw*070105 and Cw*1408, from East African women.
HLA-C was shown to be a highly polymorphic gene that can be accurately typed by sequencing methodologies. We report two novel HLA-C alleles identified during sequence-based typing of East African populations; the novel alleles were confirmed by sequencing two separate polymerase chain reaction products and by molecular cloning and sequencing multiple clones. The first new allele is identical to Cw*0701 except for a single-nucleotide synonymous substitution at codon 182 (GCA-->GCG). The new allele has been named by the WHO nomenclature committee as Cw*070105. The second new allele is identical to Cw*1403 except for a nonsynonomous change at codon 21 (CAC-->CGC), changed from histidine to arginine. The new allele has been named by the WHO nomenclature committee as Cw*1408.
Keynan
Yoav
Y
Department of Medical Microbiology, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, Canada.
Luo
Ma
M
Lacap
Phillip
P
Brunham
Robert
R
Plummer
Francis A
FA
D000483:Alleles; D001483:Base Sequence; D044383:Black People / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D007630:Kenya; D008969:Molecular Sequence Data; D013636:Tanzania
D015236:HLA-C Antigens; C512793:HLA-C*07:01:05 antigen; C512794:HLA-C*14:08 antigen