[Comparison of Epstein-Barr virus in nasopharynx tissue and desquamatine cell for diagnosis of nasopharyngeal carcinoma].
OBJECTIVE
To develop a method for rapid screening and primary diagnosis of the nasopharyngeal carcinoma (NPC).


METHODS
We detected the Epstein Barr virus (EBV) DNA from nasopharynx tissue and desquamative cells in 144 suspicious NPC patients using polymerase chain reaction (PCR). The results were compared with the pathologic diagnosis.


RESULTS
The sensitivities were 92.16% and 86.27% respectively, the specificities were 80.95% and 71.43% respectively. There was no significant difference between the results determined by the EBV DNA from nasopharynx tissue and desquamative cells using PCR.


CONCLUSIONS
The detection of EBV DNA from nasopharynx tissue and desquamative cells of the NPC by PCR is a simple, safe, rapid, specific and sensitive technique. It is useful in screening, primary diagnosis, evaluation of the curative effect of the NPC.
Sun
Zhengliang
Z
Department of Otolaryngology, the 163rd Hospital of PLA, Changsha, 410003, China.
Xiao
Chuangqing
C
Jiang
Wen
W
Feng
Xiurong
X
Liu
Yangyun
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis; D020031:Epstein-Barr Virus Infections; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000821:virology*; D009305:Nasopharynx / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D055815:Young Adult
D004279:DNA, Viral