Glucose transporter 8 (GLUT8) from the red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae).
We have cloned the fire ant glucose transporter 8 (GLUT8) cDNA providing the first molecular characterization of a GLUT8 in insects. Glucose is a poly-alcohol and, due to its high hydrophilicity, cannot move across cell membranes. GLUT8 is a putative facilitative transporter for the cellular import and export of glucose. The complete 2,974-bp cDNA encodes a 501-residue protein with a predicted molecular mass of 54.8 kDa. Transcripts were detected in the brain, midgut, hindgut, Malpighian tubule, fat body, ovary, and testis. The highest transcriptional expression was found in fat body. Northern blot analysis revealed different transcript sizes in mated queen brains, alate female ovaries, and male testes. We propose that four other sequences obtained from insect genome projects from the honey bee Apis mellifera (ENSAPMP00000006624), the malaria mosquito Anopheles gambiae (EAA11842), and the fruit fly Drosophila melanogaster (AAQ23604 and AAM52591) are likely the orthologues of the fire ant GLUT8. Phylogenetic relationships in insect glucose transporters are presented.
Chen
Mei-Er
ME
Department of Entomology, Texas A&M University, College Station, 77843-2475, USA.
Holmes
Steven P
SP
Pietrantonio
Patricia V
PV
D000595:Amino Acid Sequence; D000825:Animal Structures / Q000737:chemistry; D000818:Animals; D001000:Ants / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002417:Cattle; D003285:Contractile Proteins / Q000096:biosynthesis; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D005260:Female; D064448:Filamins; D015870:Gene Expression / Q000235:genetics / Q000502:physiology*; D051246:Glucose Transport Proteins, Facilitative / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008840:Microfilament Proteins / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
D003285:Contractile Proteins; D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D064448:Filamins; D051246:Glucose Transport Proteins, Facilitative; D008840:Microfilament Proteins; C405325:SLC2A8 protein, human