title: [Determination of amentoflavone in 11 species of Selaginella medicinal material by HPLC].
abstract: OBJECTIVE
  To determine the content of amentoflavone in 11 species of Selaginella medicinal materials.
  
  
  METHODS
  HPLC was used with kromasil (250 x 4. 6 mm, 5 microm) column. The mobile phase consisted of methanol and phosphate buffer. The gradient condition was 0-->28 min with methanol 50%-->58%, and 30-->35 min with methanol 58%-->70%. The column temperature was 40 degrees C. The flow rate was 1. 0 ml/min and the detection wavelength at 330 nm.
  
  
  RESULTS
  Amentoflavone showed a good linearity in the range of 153 - 767 ng. The average recovery was 97.03% and RSD was 0. 80% (n = 5).
  
  
  CONCLUSIONS
  The method was rapid, accurate and can be used for quality control of Selaginella medicinal materials.
authors.affiliation: Guangdong Province Institute of T. C. M. , Guangzhou 510095, China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Sun
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Luo
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Li
mesh_terms: D044946:Biflavonoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D032503:Selaginellaceae / Q000737:chemistry* / Q000145:classification
chemical_list: D044946:Biflavonoids; C011164:amentoflavone