Structure-function relations of carbohydrates by neoglycolipid arrays.
The work presented herein is a new noncovalent glycoarray assembly method for microplates created by simply mixing together a carbohydrate and a tetradecylamine. alpha-D-Mannopyranoside, alpha-D-glucopyranoside, and alpha-D-galactopyranoside were utilized in model studies and product formations were detected by lectin binding. The method can be extended to study the steric hindrance effect of carbohydrate-protein interactions, namely the structure-function relations of carbohydrates.
Huang
Gang-Liang
GL
School of Life Science, Shandong University, Jinan City, Jinan 250100, China. hgl226@126.com
Huang
Hua-Liang
HL
Zhang
Hou-Cheng
HC
Wang
Peng-George
PG
D000588:Amines / Q000737:chemistry; D001665:Binding Sites; D002241:Carbohydrates / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008358:Mannose / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship
D000588:Amines; D002241:Carbohydrates; D005960:Glucosides; D006017:Glycolipids; D037102:Lectins; D011506:Proteins; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate; C020514:N-tetradecylamine; D008358:Mannose; D005690:Galactose