ELISE NMR: experimental liquid sealing of NMR samples.
We present a simple, generally applicable approach to prevent sample evaporation when working at elevated temperatures in high resolution NMR. It consists of experimentally sealing the NMR sample by a second liquid (Experimental Liquid Sealing, ELISE). For aqueous samples, we identified the mineral oil commonly used in PCR application as the best candidate, because it contains only a very limited amount of water-soluble contaminants, is stable over time and heat resistant. The procedure does not interfere with shim settings, and is compatible with a wide variety of samples, including oligosaccharides and proteins. For chloroform samples, a simple drop of water allows to efficiently seal the sample, avoiding solvent evaporation even over lengthy time periods.
Wieruszeski
Jean-Michel
JM
CNRS-Université de Lille 1 UMR 8576, USTL, Villeneuve d'Ascq, France.
Landrieu
Isabelle
I
Hanoulle
Xavier
X
Lippens
Guy
G
D001426:Bacterial Proteins / Q000737:chemistry; D004247:DNA / Q000032:analysis; D004655:Emulsions / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009821:Oils / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011134:Polysaccharides / Q000737:chemistry; D012815:Signal Processing, Computer-Assisted; D012997:Solvents / Q000737:chemistry
D001426:Bacterial Proteins; D004655:Emulsions; D009821:Oils; D011134:Polysaccharides; D012997:Solvents; D004247:DNA