title: Diversity of gammadelta T cells in patients with Behcet's disease is indicative of polyclonal activation.
abstract: OBJECTIVE
  Behcet's disease (BD) is a multisystemic disease, with vasculitic lesions in the oral and genital mucosa, eyes, joints, skin and brain. We have previously found that gammadelta T cells are increased in peripheral blood of BD patients. The aim of this study was to investigate the extent of gammadelta T cells in oral biopsies from BD patients with special emphasis on the restriction of Vgamma and Vdelta usage.
  
  
  METHODS
  Expression of Vgamma and Vdelta chains on peripheral blood gammadelta T cells from 31 BD patients and 19 healthy controls was analysed by flow cytometry and the expression of Vgamma and Vdelta chains in nine ulcerated and eight non-ulcerated oral mucosa from BD patients and non-ulcerated oral mucosa from three healthy controls was analysed by immunohistochemistry.
  
  
  RESULTS
  Vgamma9 and Vdelta2 were the predominant chains expressed in peripheral blood of BD patients, although other Vgamma and Vdelta chains were also expressed. The presence of gammadelta T cells was only observed in the ulcerated oral mucosa but not in the non-ulcerated mucosa from the BD patients, and not in the non-ulcerated mucosa from the healthy controls. These gammadelta T cells showed no preferential expression of any of the Vgamma or Vdelta chains.
  
  
  CONCLUSIONS
  These data suggest a polyclonal rather than oligoclonal activation of the gammadelta T cells. This may indicate that during repeated inflammation of the oral mucosa, the gammadelta T cells are responding to a wide variety of antigenic stimuli with consequent expansion of gammadelta T cells expressing various Vgamma and Vdelta chains and that different antigenic stimuli or responses may be responsible for the clinical heterogeneity of the disease.
authors.affiliation: Department of Oral Medicine and Pathology, GKT Dental Institute, King's College, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Freysdottir
authors.forename: L
authors.initials: L
authors.lastname: Hussain
authors.forename: I
authors.initials: I
authors.lastname: Farmer
authors.forename: S-H
authors.initials: SH
authors.lastname: Lau
authors.forename: F
authors.initials: F
authors.lastname: Fortune
mesh_terms: D000940:Antigenic Variation; D001528:Behcet Syndrome / Q000097:blood / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology; D016022:Case-Control Studies; D006801:Humans; D007150:Immunohistochemistry; D008213:Lymphocyte Activation; D009061:Mouth Mucosa / Q000166:cytology / Q000276:immunology*; D019226:Oral Ulcer / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D017252:CD3 Complex; D016692:Receptors, Antigen, T-Cell, gamma-delta