title: Levels of detection of Cryptosporidium oocysts in mussels (Mytilus galloprovincialis) by IFA and PCR methods.
abstract: Cryptosporidium spp. are monoxenous protozoan parasites that cause gastrointestinal diseases in humans and animals. Shellfish harvesting areas can become contaminated by the infectious stage of the parasite and humans are therefore at risk of infection either by consumption of shellfish, or by taking part in recreational activities in these areas. In the present study we determined the levels of detection, by IFA and PCR techniques, of Cryptosporidium oocysts in mussels experimentally contaminated with a theoretical number of oocysts. There was a significant correlation between the results obtained by both techniques (P<0.05). IFA and PCR were also applied to a total of 222 samples of mussels (Mytilus galloprovincialis) destined for human consumption. In the naturally contaminated samples, we detected a 31.1% of contamination and only Cryptosporidium parvum (previously denominated C. parvum genotype II) was identified.
authors.affiliation: Laboratorio de Parasitología, Departamento de Microbiología y Parasitología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, La Coruña, Spain.
authors.forename: Hipólito
authors.initials: H
authors.lastname: Gómez-Couso
authors.forename: Fernando
authors.initials: F
authors.lastname: Méndez-Hermida
authors.forename: Elvira
authors.initials: E
authors.lastname: Ares-Mazás
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000469:parasitology*; D003257:Consumer Product Safety; D003458:Cryptosporidium / Q000302:isolation & purification*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000662:veterinary*; D016266:Food Parasitology; D006801:Humans; D033661:Oocysts / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000469:parasitology*