The capacity of mesenchymal stem cells for neural differentiation in vitro.
It has been shown that mesenchymal stem cells (MSCs) of bone marrow from newborn rabbits can be induced for neuronal differentiation. The epidermal growth factor (EGF) introduced in the culture at the rate of 2 ng/ml is able to promote differentiation of neurons from bone marrow mesenchymal stem cells in 27 days of cultivation. Differentiated cells were marked by monoclonal antibodies to 70 kDa neurofilaments. The data obtained show a possibility of using bone marrow stem cells in therapy of neurodegenerative diseases.
Zemchikhina
V N
VN
Institute of Gene Biology, Moscow. vellbiogen@mail.ru
Golubeva
O N
ON
D000818:Animals; D000831:Animals, Newborn; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D004815:Epidermal Growth Factor; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008970:Molecular Weight; D016900:Neurofilament Proteins / Q000032:analysis / Q000737:chemistry; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011817:Rabbits; D013997:Time Factors
D003470:Culture Media; D016900:Neurofilament Proteins; D004815:Epidermal Growth Factor