title: Anti-CXCR3 staining is useful for detecting human cutaneous and mucosal mast cells.
abstract: Human synovial mast cells (MC) can be immunolabelled with antihuman CXCR3 antibody (Ab) (clone 49801). We have investigated whether cutaneous and mucosal MC are stained with anti-CXCR3 Ab in paraffin-embedded sections. Immunohistochemical staining and immunofluorescence double staining assays were performed with anti-CXCR3, anti-tryptase, and anti-chymase Ab using normal skin, psoriatic skin lesions, and normal colon. When compared with tryptase and chymase staining, 100% of the cutaneous and 98% of the mucosal MC were positive for CXCR3. Anti-CXCR3 staining is a useful marker for human cutaneous and mucosal MC in paraffin-embedded sections.
authors.affiliation: Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. norikof@dermatol.med.kyushu-u.ac.jp
authors.forename: Noriko
authors.initials: N
authors.lastname: Fukiwake
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Moroi
authors.forename: Shinichi
authors.initials: S
authors.lastname: Imafuku
authors.forename: Teiichi
authors.initials: T
authors.lastname: Masuda
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kokuba
authors.forename: Masutaka
authors.initials: M
authors.lastname: Furue
authors.forename: Kazunori
authors.initials: K
authors.lastname: Urabe
mesh_terms: D000911:Antibodies, Monoclonal; D003106:Colon / Q000166:cytology; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D008407:Mast Cells / Q000378:metabolism*; D016612:Paraffin Embedding; D011565:Psoriasis / Q000473:pathology*; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000276:immunology*; D012867:Skin / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; C515187:CXCR3 protein, human; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine