title: [Protective effect of lidocaine on injury alveolar Type II cells induced by LPS in adult rats].
abstract: OBJECTIVE
  To investigate the effect of lidocaine on LPS induced apoptosis of cultured adult rat alveolar Type II (AT-II) cells.
  
  
  METHODS
  Cultured cells were exposed to LPS and lidocaine for 24 hours. Apoptosis and necrosis rates of cells were detected by flow cytometry and electron microscope. The activity of lactic dehydrogenase (LDH) was analyzed by using LDH kits.
  
  
  RESULTS
  LPS induced the AT-II cell injuries by increasing not only the necrosis and apoptosis rates but also the LDH release of cultured AT-II in vitro. Lidocaine decreased the necrosis and apoptosis rates of AT-II cells.
  
  
  CONCLUSIONS
  Lidocaine can directly inhibit the apoptosis and necrosis induced by LPS in cultured AT-II cells.
authors.affiliation: Department of ICU, Xiangya Hospital, Central South University, Changsha, China. xudaomiao@medmail.com.cn
authors.forename: Dao-miao
authors.initials: DM
authors.lastname: Xu
authors.forename: Guang-feng
authors.initials: GF
authors.lastname: Ming
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Wu
authors.forename: Sheng-xi
authors.initials: SX
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000473:pathology*; D005434:Flow Cytometry; D045022:Lactate Dehydrogenases / Q000378:metabolism; D008012:Lidocaine / Q000494:pharmacology*; D008070:Lipopolysaccharides; D009336:Necrosis; D020011:Protective Agents / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008070:Lipopolysaccharides; D020011:Protective Agents; D008012:Lidocaine; D045022:Lactate Dehydrogenases