A comparison of enhanced green fluorescent protein expression induced by immediate-early cytomegalovirus (IE-CMV) and gG pseudorabies virus (gG-PRV) promoters, using pseudorabies virus amplicons as vectors.
This study compares the expression efficiencies of the IE-CMV and gG-PRV promoters following their transfection into cultured human and monkey cells, using pseudorabies virus amplicons as vectors and enhanced green fluorescence protein (EGFP) as an expression marker. EGFP expression was similarly strong with both promoters. Pseudorabies virus amplicons appear to be useful vectors in gene expression studies due to their replication in the presence of helpers and their wide range of cellular hosts.
Muñoz
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AL
Departamento de Medicina Preventiva, Salud Pública y Microbiología, Facultad de Medicina, Universidad Autónoma de Madrid, Arzobispo Morcillo 4, E-28029 Madrid, Spain.
Prieto
C
C
Tabarés
E
E
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D000882:Haplorhini; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*
D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D014759:Viral Envelope Proteins; C485184:enhanced green fluorescent protein; C044582:immediate-early proteins, cytomegalovirus; C042966:pseudorabies virus glycoproteins; D049452:Green Fluorescent Proteins