Isolation and sequence analysis of the Sox-1, -2, -3 homologs in Trionyx sinensis and Alligator sinensis having temperature-dependent sex determination.
Members of the Sox gene family are characterized by an HMG-box that shows sequence similarity with that of the mouse testis-determining gene Sry. Using degenerate primers PCR, seven and eight HMG-box motifs of Sry-related genes were cloned and sequenced from genomic DNA of Trionyx sinensis (termed TS41-47) and Alligator sinensis (AS41-48) with TSD (temperature-dependent sex determination). Among 15 Sry-related genes, TS41, TS42, AS41, and AS42 shared 80, 72, 81, and 79% amino acid identity, respectively, with each HMG-box domain of the mouse Sox-1, -2, and -3 genes by Blast analysis. Molecular phylogenetic analysis showed that the clustering of TS41-42 and AS41-42 was distant to the clustering of the nonreptilian vertebrate Sox-1, -2, -3 homologs, including fish, amphibian, bird, and mammals. The amino acid identity among TS41-42, AS41-42, and the nonreptilian vertebrate Sox-1, -2, -3 homologs is lower than identities among the Sox-1, -2, -3 homologs, suggesting that the sequence changes in TS41-42 of Trionyx sinensis and AS41-42 of Alligator sinensis might have occurred after the diversification of amniotes.
Zheng
Jifang
J
Instiute of Geneties, College of Life Sciences, Zhejiang University, Hangzhou 310012, P.R. China.
Zhu
Muyuan
M
D000489:Alligators and Crocodiles / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D024282:Genes, sry / Q000235:genetics; D024742:HMG-Box Domains / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D019849:Sex Determination Processes; D051876:Sex-Determining Region Y Protein / Q000235:genetics*; D013696:Temperature; D014426:Turtles / Q000145:classification* / Q000235:genetics* / Q000254:growth & development
D051876:Sex-Determining Region Y Protein