title: A new format of electrodes for the electrochemical reduction of cytochromes P450.
abstract: New approach to the electrochemical reduction of cytochromes P450 (P450s, CYPs) at electrodes chemically modified with appropriate substrates for P450s ("reverse" electrodes) was proposed. The method is based on the analysis of cyclic voltammograms, square-wave voltammograms and amperograms with subsequent determination of electrochemical characteristics such as catalytic current and redox potential. The sensitivity of proposed method is 0.2-1 nmol P450/electrode. The changes of maximal current and of redox potentials in square-wave voltammograms as well as the changes of catalytic current in amperometric experiments proved to be informative and reliable. Planar regime of screen-printed electrodes (strip-type sensors) enabled to utilise 20-60 microl of electrolyte volume. The enzyme-substrate pairs P450 2B4/benzphetamine and P450scc/cholesterol were investigated. Electrochemical parameters of electrodes with unspecific P450 substrates differed considerably from electrodes with appropriate substrates.
authors.affiliation: Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya Street, 10, Moscow 119121, Russia. viktoria.shumyantseva@ibmc.msk.ru
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Shumyantseva
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Bulko
authors.forename: Natalia F
authors.initials: NF
authors.lastname: Samenkova
authors.forename: Galina P
authors.initials: GP
authors.lastname: Kuznetsova
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Usanov
authors.forename: Holger
authors.initials: H
authors.lastname: Schulze
authors.forename: Till T
authors.initials: TT
authors.lastname: Bachmann
authors.forename: Rolf D
authors.initials: RD
authors.lastname: Schmid
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D015374:Biosensing Techniques; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D010084:Oxidation-Reduction
chemical_list: D003577:Cytochrome P-450 Enzyme System