The interaction between N-n-undecyl-N'-(sodium p-aminobenzenesulfonate) thiourea and serum albumin studied using various spectroscopies and the molecular modeling method.
The preparation and characteristics of N-n-undecyl-N'-(sodium-p-aminobenzenesulfonate) thiourea (UPT), a new water-soluble reagent with a saturated fatty hydrocarbon group, were described. The interactions of UPT with bovine serum albumin (BSA) and human serum albumin (HSA) were studied using fluorescence spectroscopy in combination with ultraviolet (UV) absorption spectroscopy, circular dichroism (CD) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and the molecular modeling method. UPT exhibited a strong ability to quench the intrinsic fluorescence of both BSA and HSA through a static quenching procedure. The binding constants of UPT and BSA or HSA were determined at different temperatures based on the relevant fluorescence data. The binding sites were obtained and the acting force was suggested to be mainly hydrophobic interaction, which was consistent with the result of the molecular modeling study, and there were also a number of hydrogen bonds between UPT and HSA. The results of determination of the proteins in bovine serum or human serum by this method were very close to those obtained by using Coomassie Brilliant Blue G-250 colorimetry. A practical method was proposed for the determination of UPT in bovine serum or human serum samples with satisfactory results.
Cui
Fengling
F
Henan Key Laboratory for Environmental Pollution Control, College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, Henan 453007, China. fenglingcui@hotmail.com
Cui
Yanrui
Y
Luo
Hongxia
H
Yao
Xiaojun
X
Fan
Jing
J
Lu
Yan
Y
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D012709:Serum Albumin / Q000032:analysis* / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013425:Sulfanilic Acids / Q000737:chemistry / Q000378:metabolism*; D013890:Thiourea / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
C510556:N-n-undecyl-N'-(4-aminobenzenesulfonic acid)thiourea; D012709:Serum Albumin; D013425:Sulfanilic Acids; D012710:Serum Albumin, Bovine; D013890:Thiourea