The native anti-glucocorticoid paradigm.
Circulating 3beta-hydroxysteroids including dehydroepiandrosterone (DHEA) are 7alpha-hydroxylated by the cytochrome P450-7B1 in the liver, skin and brain, which are the target organs of glucocorticoids. Anti-glucocorticoid effects with 7alpha-hydroxy-DHEA were observed in vivo without an interference with glucocorticoid binding to its receptor. In the organs mentioned above, the circulating inactive cortisone was reduced into active cortisol by the 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1). We demonstrated that 7alpha-hydroxy-DHEA was also a substrate for this enzyme. Studies of the 11beta-HSD1 action on 7alpha-hydroxy-DHEA showed the reversible production of 7beta-hydroxy-DHEA through an intermediary 7-oxo-DHEA, and the kinetic parameters favored this production over that of active glucocorticoids. Both the production of 7alpha-hydroxysteroids and their interference with the activation of cortisone into cortisol are basic to the concept of native anti-glucocorticoids efficient at their production site. This opens a promising new area for research.
Muller
Caroline
C
Laboratoire de Biotechnologie, EA 3199, Conservatoire National des Arts et Métiers, 2 rue Conté, 75003 Paris, France.
Hennebert
Olivier
O
Morfin
Robert
R
D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000235:genetics / Q000378:metabolism; D000375:Aging / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D003348:Cortisone / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D000072474:Cytochrome P450 Family 7; D003687:Dehydroepiandrosterone / Q000378:metabolism*; D005938:Glucocorticoids / Q000037:antagonists & inhibitors*; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008954:Models, Biological; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism; D013379:Substrate Specificity
D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D003687:Dehydroepiandrosterone; D003577:Cytochrome P-450 Enzyme System; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D013250:Steroid Hydroxylases; D000072474:Cytochrome P450 Family 7; C499571:CYP7B1 protein, human; C097207:Cyp7b1 protein, mouse; C490784:Cyp7b1 protein, rat; D003348:Cortisone; D006854:Hydrocortisone