title: Oxygen radical formation does not have an impact in the treatment of severe acute experimental pancreatitis using free cellular hemoglobin.
abstract: OBJECTIVE
  Microcirculatory dysfunction and free oxygen radicals are important factors in the pathogenesis of severe acute pancreatitis. Additional oxygen delivery might enhance lipid peroxidation but may also improve pancreatic microcirculation. This study assesses the effect of free cellular bovine hemoglobin on the formation of oxygen radicals and microcirculation in a rodent model of severe acute pancreatitis.
  
  
  METHODS
  Fifteen minutes after induction of acute pancreatitis Wistar rats received either 0.8 mL bovine hemoglobin (HBOC-200), hydroxyethyl starch (HES) or 2.4 mL of normal saline to ensure normovolemic substitution. After 6 h of examination the pancreas was excised and rapidly processed for indirect measurement of lipid peroxidation products malondialdehyde (MDA) and reduced glutathione (GSH) in pancreatic tissue.
  
  
  RESULTS
  The single application of HBOC-200 improved pancreatic microcirculation and reduced histopathological tissue damage significantly. Tissue concentration of MDA did not differ between the groups. Also no differences in GSH levels were detected.
  
  
  CONCLUSIONS
  Though the single application of HBOC-200 and HES improve pancreatic microcirculation, no differences in lipid peroxidation products were detected. The beneficial effect of additional oxygen supply (HBOC-200) does not lead to enhanced lipid peroxidation.
authors.affiliation: Department of General-, Visceral- and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany.
authors.forename: Helge
authors.initials: H
authors.lastname: Kleinhans
authors.forename: Oliver
authors.initials: O
authors.lastname: Mann
authors.forename: Paulus G
authors.initials: PG
authors.lastname: Schurr
authors.forename: Jussuf T
authors.initials: JT
authors.lastname: Kaifi
authors.forename: Bente
authors.initials: B
authors.lastname: Hansen
authors.forename: Jakob R
authors.initials: JR
authors.lastname: Izbicki
authors.forename: Tim
authors.initials: T
authors.lastname: Strate
mesh_terms: D000208:Acute Disease; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006454:Hemoglobins / Q000494:pharmacology / Q000627:therapeutic use*; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology / Q000627:therapeutic use; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008833:Microcirculation / Q000502:physiology; D010179:Pancreas / Q000098:blood supply / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D010195:Pancreatitis / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D010952:Plasma Substitutes / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012039:Regional Blood Flow / Q000502:physiology
chemical_list: D006454:Hemoglobins; D006895:Hydroxyethyl Starch Derivatives; D010952:Plasma Substitutes; D017382:Reactive Oxygen Species; C402601:hemoglobin glutamer-200