Pre-B-cell colony-enhancing factor (PBEF/Visfatin) gene expression is modulated by NF-kappaB and AP-1 in human amniotic epithelial cells.
A localized intrauterine inflammatory response is often associated with the initiation of normal human parturition, whereas infection causes a similar but more florid response initiating preterm labor. Pre-B-cell colony-enhancing factor (PBEF) is expressed in the human fetal membranes and is up-regulated by labor, severe infection and inflammatory stimuli. The aim of this study was to determine the involvement of the transcription factors NF-kappaB and AP-1 in the response of PBEF to an inflammatory stimulus and compare it with IL-8. The results showed that this treatment of amniotic epithelial-like cells (WISH) and primary amniotic epithelial cells increased expression of PBEF and IL-8, but IL-8 responded 100-fold more than PBEF. IL-1beta treatment together with a panel of NF-kappaB and AP-1 inhibitors demonstrated the involvement of these transcription factors in the up-regulation of PBEF. These data show that an inflammatory stimulus in the fetal membranes inducing NF-kappaB and AP-1 would up-regulate PBEF as well as IL-8.
Kendal
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Developmental and Reproductive Biology, University of Hawaii, 651 Ilalo Street, Biosciences Building, John A. Burns School of Medicine, Honolulu, HI 96813, USA. kendal@pbrc.hawaii.edu
Bryant-Greenwood
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D000650:Amnion / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002452:Cell Count; D002467:Cell Nucleus / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D016209:Interleukin-8 / Q000378:metabolism; D016328:NF-kappa B / Q000494:pharmacology*; D054409:Nicotinamide Phosphoribosyltransferase; D018808:Transcription Factor AP-1 / Q000494:pharmacology*; D015854:Up-Regulation
D016207:Cytokines; D053583:Interleukin-1beta; D016209:Interleukin-8; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human