title: Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker.
abstract: [reaction: see text] We report the development of a safety-catch photolabile linker that allows the light-directed synthesis and spatially selective photorelease of oligonucleotides from microarrays. The linker remains stable to light during DNA synthesis, and is activated for photorelease after acidic hydrolysis. We demonstrate that the photoreleased oligonucleotides can be amplified by PCR to produce double stranded DNA. The advantages offered by this linker could aid the development of an automated gene synthesis platform.
authors.affiliation: The Department of Chemistry, University of Wisconsin-Madison, 53706, USA.
authors.forename: Shane T
authors.initials: ST
authors.lastname: Flickinger
authors.forename: Madhusudan
authors.initials: M
authors.lastname: Patel
authors.forename: Brock F
authors.initials: BF
authors.lastname: Binkowski
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Lowe
authors.forename: Mo-Huang
authors.initials: MH
authors.lastname: Li
authors.forename: Changhan
authors.initials: C
authors.lastname: Kim
authors.forename: Franco
authors.initials: F
authors.lastname: Cerrina
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Belshaw
mesh_terms: D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010782:Photolysis
chemical_list: D009841:Oligonucleotides; D004247:DNA