Antihypertensive effect of angiotensin I-converting enzyme inhibitory peptides from a sesame protein hydrolysate in spontaneously hypertensive rats.
Sesame peptide powder (SPP) exhibited angiotensin I-converting enzyme (ACE) inhibitory activity, and significantly and temporarily decreased the systolic blood pressure (SBP) in spontaneously hypertensive rats (SHRs) by a single administration (1 and 10 mg/kg). Six peptide ACE inhibitors were isolated and identified from SPP. The representative peptides, Leu-Val-Tyr, Leu-Gln-Pro and Leu-Lys-Tyr, could competitively inhibit ACE activity at respective Ki values of 0.92 microM, 0.50 microM, and 0.48 microM. A reconstituted sesame peptide mixture of Leu-Ser-Ala, Leu-Gln-Pro, Leu-Lys-Tyr, Ile-Val-Tyr, Val-Ile-Tyr, Leu-Val-Tyr, and Met-Leu-Pro-Ala-Tyr according to their content ratio in SPP showed a strong antihypertensive effect on SHR at doses of 3.63 and 36.3 microg/kg, which accounted for more than 70% of the corresponding dosage for the SPP-induced hypotensive effect. Repeated oral administration of SPP also lowered both SBP and the aortic ACE activity in SHR. These results demonstrate that SPP would be a beneficial ingredient for preventing and providing therapy against hypertension and its related diseases.
Nakano
Daisuke
D
Department of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Ogura
Kyoichi
K
Miyakoshi
Masazumi
M
Ishii
Fumie
F
Kawanishi
Hideaki
H
Kurumazuka
Daisuke
D
Kwak
Chol-Jun
CJ
Ikemura
Kenji
K
Takaoka
Masanori
M
Moriguchi
Shigeo
S
Iino
Taeko
T
Kusumoto
Aki
A
Asami
Sumio
S
Shibata
Hiroshi
H
Kiso
Yoshinobu
Y
Matsumura
Yasuo
Y
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D006868:Hydrolysis; D006973:Hypertension / Q000201:enzymology*; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007703:Peptidyl-Dipeptidase A / Q000187:drug effects*; D028321:Plant Preparations / Q000008:administration & dosage / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D011918:Rats, Inbred SHR; D031685:Sesamum / Q000737:chemistry*
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D009842:Oligopeptides; D028321:Plant Preparations; D010940:Plant Proteins; D007703:Peptidyl-Dipeptidase A