Freeze-drying of human platelets: influence of intracellular trehalose and extracellular protectants.
Freeze-drying is an ideal alternative for long-term preservation of platelets in blood banks. Intracellular trehalose is believed to be an effective lyoprotectant for preserving cells during freeze-drying. In this study, 13 mM intracellular trehalose was loaded into human platelets through fluid-phase endocytosis pathway. Bovine serum albumin and trehalose were used as extracellular protectants. The effects of intracellular trehalose and extracellular protectants on freeze-dried platelets were studied respectively. The results showed 13 mM intracellular trehalose was beneficial to freeze-dried human platelets, but only slightly enhanced the protection afforded by extracellular protectants. Loaded with 13 mM intracellular trehalose, platelets were freeze-dried in a formulation of 1 percent bovine serum albumin and 1 percent trehalose, 40 days later, the survival rate of rehydrated platelets was about 85 percent, the morphology of rehydrated platelets was intact and the aggregation percentage with thrombin (1 U/ml) was 97.3 percent.
Zhou
Xin-Li
XL
Cryobiology laboratory, Institute of Refrigeration and Cryogenic Engineering, Zhejiang University, Hangzhou, China.
Zhu
Hong
H
Zhang
Shao-Zhi
SZ
Zhu
Fa-Ming
FM
Chen
Guang-Ming
GM
Yan
Li-Xing
LX
D001792:Blood Platelets / Q000187:drug effects*; D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D019610:Cytoprotection / Q000187:drug effects*; D045604:Extracellular Fluid / Q000187:drug effects; D005612:Freeze Drying / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects; D009994:Osmolar Concentration; D010974:Platelet Aggregation / Q000187:drug effects; D014199:Trehalose / Q000494:pharmacology*
D003451:Cryoprotective Agents; D014199:Trehalose