title: The zebrafish bHLH PAS transcriptional regulator, single-minded 1 (sim1), is required for isotocin cell development.
abstract: A wide range of physiological and behavioral processes, such as social, sexual, and maternal behaviors, learning and memory, and osmotic homeostasis are influenced by the neurohypophysial peptides oxytocin and vasopressin. Disruptions of these hormone systems have been linked to several neurobehavioral disorders, including autism, Prader-Willi syndrome, affective disorders, and obsessive-compulsive disorder. Studies in zebrafish promise to reveal the complex network of regulatory genes and signaling pathways that direct the development of oxytocin- and vasopressin-like neurons, and provide insight into factors involved in brain disorders associated with disruption of these systems. Isotocin, which is homologous to oxytocin, is expressed early, in a simple pattern in the developing zebrafish brain. Single-minded 1 (sim1), a member of the bHLH-PAS family of transcriptional regulatory genes, is required for terminal differentiation of mammalian oxytocin cells and is a master regulator of neurogenesis in Drosophila. Here we show that sim1 is expressed in the zebrafish forebrain and is required for isotocin cell development. The expression pattern of sim1 mRNA in the embryonic forebrain is dynamic and complex, and overlaps with isotocin expression in the preoptic area. We provide evidence that the role of sim1 in zebrafish neuroendocrine cell development is evolutionarily conserved with that of mammals.
authors.affiliation: Graduate Program in Cellular and Molecular Biology, School of Biomedical Sciences, Kent State University, Kent, Ohio, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Eaton
authors.forename: Eric
authors.initials: E
authors.lastname: Glasgow
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D019521:Body Patterning; D002454:Cell Differentiation; D004027:Diencephalon / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000378:metabolism; D010802:Phylogeny; D016548:Prosencephalon / Q000196:embryology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013126:Spinal Nerve Roots / Q000196:embryology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D010121:Oxytocin; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins; C491092:Sim1a protein, zebrafish; C008215:isotocin