[Effect on apoptosis of Tca8113 of interference of Bcl-2 and HER-2 genes antisense oligodeoxynucleotides transfection].
OBJECTIVE
To investigate the interference and the mechanisms of Bcl-2 and HER-2 genes antisense oligodeoxynucleotides combined transfection in the human tongue carcinoma Tca8113 cell lines.


METHODS
There were 6 groups in our study, normal control group, Bcl-2 sense experimental group, HER-2 sense experimental group, Bcl-2 antisense experimental group, HER-2 antisense experimental group, Bcl-2 and HER-2 genes antisense oligodeoxynucleotides combined transfection experimental group. In the different times after liposome-mediated transfection, the cell apoptosis, Bcl-2 and HER-2 expressing level were observed by RT-PCR and electronic microscope.


RESULTS
According to the results of combined transfection experimental group, the apoptosis body and apoptosis cells were observed. The expression of genes were decreased statistically in both Bcl-2 and HER-2, respectively. Bcl-2 and HER-2 combined ASODN was superior to single ASODN in the intervention of tongue carcinoma.


CONCLUSIONS
Bcl-2 and HER-2 ASODN can effectively interfere the expression of HER-2 and Bcl-2 genes. The expression of HER-2 and Bcl-2 can be reduced, and the apoptosis of cells can be enhanced.
Ji
Ping
P
Dept. of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China.
Wu
Yong-Zhong
YZ
Zhang
Fu-Jun
FJ
Lin
Shao-Lin
SL
D017209:Apoptosis; D005260:Female; D018734:Genes, erbB-2; D006801:Humans; D009838:Oligodeoxyribonucleotides; D020319:Oligodeoxyribonucleotides, Antisense; D014162:Transfection
D009838:Oligodeoxyribonucleotides; D020319:Oligodeoxyribonucleotides, Antisense