Expression of active alpha-N-acetylglucosaminidase/TAT Chimerae in cultured Spodoptera frugiperda cells.
We examined the production and secretion of fusion constructs containing alpha-N-acetylglucosaminidase, the enzyme deficient in Sanfilippo B, and either wildtype TAT or modified TAT in cultured Spodoptera frugiperda cells. All constructs exhibited successful expression of active enzyme, suggesting the future possibility of utilizing TAT/alpha-N-acetylglucosaminidase chimerae in enzyme replacement therapy.
Bandsmer
Judith C
JC
Southern Alberta Cancer Research Institute, Dept. of Oncology, University of Calgary, Calgary, AB, Canada.
Campbell
Tessa N
TN
Cheyne
Ian
I
Choy
Francis Y M
FY
D000118:Acetylglucosaminidase / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D015696:Gene Products, tat / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D018411:Spodoptera; D014161:Transduction, Genetic
D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; C033079:alpha-N-acetyl-D-glucosaminidase; D000118:Acetylglucosaminidase