Cloning and expression analysis of a murine novel gene, Ayu17-449.
We used gene trapping vector PU8 to search some interesting genes which play important roles in mouse development from murine ES cells. One positive ES colony termed Ayu17-449 was trapped. Its partial cDNA was obtained by using 5' RACE method. It is homologous to a 5,523 bp cDNA fragment (GI: 20879412) in EST database. Further analysis of the 5,523 bp cDNA sequence in Celera mouse gene database showed that it overlaps two genes. We designed serials of DNA primers according to the mRNAs of these two genes for RT-PCR and Northern blotting analysis, and identified a novel RNA about 9 kb (we named it as Ayu17-449) encoding 1,920 aa. This gene is expressed highly in the brain, kidney, heart, lung, muscle and stomach. The expressed protein contains a Granin motif on its N-terminus, showing that this gene may be involved in hormone secretion.
Tang
Hua
H
Key Laboratory of Molecular Biology on Infectious Diseases, Chongqing University of Medical Sciences, China. tanghua86162003@yahoo.com.cn
Araki
Kimi
K
Yamamura
Ken-ichi
K
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002864:Chromogranins / Q000502:physiology*; D033481:Chromosomes, Mammalian; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D004622:Embryo, Mammalian / Q000166:cytology; D015870:Gene Expression; D005822:Genetic Vectors; D019868:Kanamycin Kinase / Q000235:genetics / Q000378:metabolism; D051379:Mice / Q000196:embryology / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D002864:Chromogranins; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D019868:Kanamycin Kinase; D001616:beta-Galactosidase