Adipogenic differentiation of adipose tissue derived adult stem cells in nude mouse.
We evaluated the use of a combination of adipose tissue derived adult stem cells (ADSCs) obtained from liposuction and injectable poly(lactic-co-glycolic acid) (PLGA) spheres for adipose tissue engineering. Adipogenesis was examined in nude mice injected subcutaneously with ADSCs (group I), PLGA spheres (group II), or ADSCs attached PLGA spheres (group III) cultured in adipogenic medium for 7 days. After 4 and 8 weeks, newly formed adipose tissue was observed in groups II and III but not in group I. Oil red O staining of newly formed tissue showed that there was substantially more tissue regeneration and adipogenic differentiation in group III than in group II. RT-PCR confirmed that, after 8 weeks, the PLGA-attached ADSCs had fully differentiated into adipocytes. This study provides significant evidence that ADSCs and PLGA spheres can be used in a clinical setting to generate adipose tissue as a noninvasive soft tissue filler.
Choi
Yu Suk
YS
Asan Institute for life sciences, University of Ulsan, and Sein Aesthetic Plastic Surgery Clinic, Seoul 138-736, Republic of Korea.
Cha
Sang Myun
SM
Lee
Young Yeol
YY
Kwon
Seok Woon
SW
Park
Chan Jeoung
CJ
Kim
MiJung
M
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D015187:Lipectomy; D051379:Mice; D008819:Mice, Nude; D008863:Microspheres; D010641:Phenotype; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
D011100:Polyglycolic Acid