title: Identification of informative strains and provisional QTL mapping of amphetamine (AMPH)-induced locomotion in recombinant congenic strains (RCS) of mice.
abstract: Amphetamine (AMPH)-induced locomotor activity is a rodent behavioral trait that reflects mesolimbic dopaminergic activity. To identify potential quantitative trait loci (QTL) associated with this behavior, we used 34 recombinant congenic strains (RCSs) of mice derived from A/J (A strains) and C57BL/6J (B strains) and measured AMPH-induced total distance traveled (AMPH-TDIST). Two strains in the A panel (A52 and A63) showed significantly elevated AMPH-TDIST compared to the parental A/J strain and behaved similarly to C57BL/6J. Simple sequence length polymorphism (SSLP) markers on chromosomes 1, 2, 3, 5, 6, 8, 9, 10 and 20 were significantly associated with AMPH-TDIST in the A strains. Within the B panel, two strains (B81 and B74) had significantly higher and two strains (B69 and B75) had significantly lower AMPH-TDIST than C57BL/6J. Markers associated with AMPH-TDIST in the B strains appeared on chromosomes 5, 17 and 20. Combining data from this approach and other genetic (mapping data in humans) and functional (cDNA expression) sources may help to identify suitable candidate genes relevant to human disorders where mesolimbic dopamine dysregulation has been postulated.
authors.affiliation: Douglas Hospital Research Centre, and Department of Psychiatry, McGill University, Montreal, Quebec, Canada. adam.torkaman-zehi@douglas.mcgill.ca
authors.forename: Adam
authors.initials: A
authors.lastname: Torkamanzehi
authors.forename: Patricia
authors.initials: P
authors.lastname: Boksa
authors.forename: Mouhssine
authors.initials: M
authors.lastname: Ayoubi
authors.forename: Marie-Eve
authors.initials: ME
authors.lastname: Fortier
authors.forename: N M K
authors.initials: NM
authors.lastname: Ng Ying Kin
authors.forename: Emile
authors.initials: E
authors.lastname: Skamene
authors.forename: Guy
authors.initials: G
authors.lastname: Rouleau
authors.forename: Ridha
authors.initials: R
authors.lastname: Joober
mesh_terms: D000661:Amphetamine / Q000493:pharmacokinetics / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal; D002874:Chromosome Mapping; D007700:Kinetics; D051379:Mice; D008805:Mice, Inbred A / Q000235:genetics*; D008810:Mice, Inbred C57BL / Q000235:genetics*; D009043:Motor Activity / Q000187:drug effects / Q000235:genetics*; D040641:Quantitative Trait Loci
chemical_list: D000661:Amphetamine