title: Morphology and adhesion of biomolecules on silicon based surfaces.
abstract: Biomolecules such as proteins, on silicon based surfaces are of extreme importance in various applications including microfabricated silicon implants, in the fabrication of microdevices with protein compounds (e.g., biosensors), and therapeutics. Morphology of silicon based surfaces with and without biomolecules and their adhesion with the substrate govern performance and reliability of the biological application. In this research, step by step morphological changes of silicon as well as adhesion during its surface modification have been studied using atomic force microscopy. To improve adhesion between biomolecules and the silicon based surfaces, chemical conjugation as well as surface patterning have been used. Changes in adhesion as a result of surface modification have been analyzed with the help of contact angle measurements. Phase imaging technique was used to confirm the presence of biomolecules on the surface. To understand the relationship between morphology and local values of adhesion, adhesion mapping and local stiffness mapping were carried out.
authors.affiliation: Nanotribology Laboratory for Information storage and MEMS/NEMS, The Ohio State University, 650 Ackerman Road, Suite 255, Columbus, OH 43202, USA. bhushan.2@osu.edu
authors.forename: Bharat
authors.initials: B
authors.lastname: Bhushan
authors.forename: Dharma R
authors.initials: DR
authors.lastname: Tokachichu
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Keener
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Lee
mesh_terms: D002478:Cells, Cultured; D018625:Microscopy, Atomic Force; D011506:Proteins / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011506:Proteins; D012825:Silicon