Soret spectral and bioinformatic approaches provide evidence for a critical role of the alpha -subunit in assembly of tetrameric hemoglobin.
Soret spectral contributions of the alpha-subunit heme pocket have been evaluated by performing static titrations of apohemoglobin A with CNProtohemin under varied experimental conditions. Increasing the temperature from 5 to 30 degrees C in 0.05 M potassium phosphate buffer, pH 7, resulted in a decreasingly prominent hypsochromic shifts reflecting altered the vinyl-globin interactions. Studies at 10 degrees C in over pH range of 6.7-8.0 revealed a profile for the spectral shifts approximating the side chain pK value (7.4) a histidyl residue. These overall spectral changes correspond to DeltaE of < or = 7 kJ/mol indicative of electrostatic noncovalent interactions. Further our current molecular modeling studies indicate that the spatial arrangement and critical noncovalent interactions of tyrosine 42 and histidine 45 (aromatic residues unique to the alpha-subunit) make significant contribution to the Soret spectra. Most interestingly, phylogenetic analyses have revealed the presence of a histidyl triad in the alpha-chain of all vertebrates that form heterotetramers.
Vasudevan
Gayathri
G
Department of Chemistry, University of Massachusetts Lowell, One University Avenue, Lowell, MA 01854, USA.
McDonald
Melisenda J
MJ
D000595:Amino Acid Sequence; D001665:Binding Sites; D019295:Computational Biology; D006418:Heme; D006427:Hemin / Q000378:metabolism*; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D006639:Histidine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D013057:Spectrum Analysis; D013696:Temperature
D006454:Hemoglobins; D021122:Protein Subunits; D006418:Heme; D006639:Histidine; D006427:Hemin