title: High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow.
abstract: Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l(-1). The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone.
authors.affiliation: Biosidus SA, Buenos Aires, Argentina.
authors.forename: Daniel
authors.initials: D
authors.lastname: Salamone
authors.forename: Lino
authors.initials: L
authors.lastname: Barañao
authors.forename: Claudio
authors.initials: C
authors.lastname: Santos
authors.forename: Leonardo
authors.initials: L
authors.lastname: Bussmann
authors.forename: Jorge
authors.initials: J
authors.lastname: Artuso
authors.forename: Carlos
authors.initials: C
authors.lastname: Werning
authors.forename: Aida
authors.initials: A
authors.lastname: Prync
authors.forename: Cesar
authors.initials: C
authors.lastname: Carbonetto
authors.forename: Susana
authors.initials: S
authors.lastname: Dabsys
authors.forename: Carlos
authors.initials: C
authors.lastname: Munar
authors.forename: Roberto
authors.initials: R
authors.lastname: Salaberry
authors.forename: Guillermo
authors.initials: G
authors.lastname: Berra
authors.forename: Ignacio
authors.initials: I
authors.lastname: Berra
authors.forename: Nahuel
authors.initials: N
authors.lastname: Fernández
authors.forename: Mariana
authors.initials: M
authors.lastname: Papouchado
authors.forename: Marcelo
authors.initials: M
authors.lastname: Foti
authors.forename: Norberto
authors.initials: N
authors.lastname: Judewicz
authors.forename: Ignacio
authors.initials: I
authors.lastname: Mujica
authors.forename: Luciana
authors.initials: L
authors.lastname: Muñoz
authors.forename: Silvina Fenández
authors.initials: SF
authors.lastname: Alvarez
authors.forename: Eliseo
authors.initials: E
authors.lastname: González
authors.forename: Juan
authors.initials: J
authors.lastname: Zimmermann
authors.forename: Marcelo
authors.initials: M
authors.lastname: Criscuolo
authors.forename: Carlos
authors.initials: C
authors.lastname: Melo
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D019976:Cloning, Organism; D019382:Human Growth Hormone / Q000096:biosynthesis*; D006801:Humans; D008894:Milk Proteins / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D008894:Milk Proteins; D011994:Recombinant Proteins; D019382:Human Growth Hormone