title: Histologic comparison of autologous fat processing methods.
abstract: OBJECTIVE
  To perform a quantitative analysis of adipocyte viability after fat processing during autologous fat transfer, comparing the processing methods of washing, centrifuging, and sedimentation.
  
  
  METHODS
  An experimental study was conducted in which 24 fat samples were obtained after processing from 22 patients undergoing autologous fat transfer. Histologic analysis of periodic acid-Schiff-stained specimens was then performed.
  
  
  RESULTS
  Cell counts per high-powered field of intact adipocytes and nucleated adipocytes and adipocyte cross-sectional area were significantly greater in samples processed by sedimentation, compared with those by centrifuging or washing.
  
  
  CONCLUSIONS
  Of the various processing techniques currently used during autologous fat transfer, sedimentation appears to yield a higher proportion of viable adipocytes than does washing or centrifuging.
authors.affiliation: Oculoplastics Service, Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, USA.
authors.forename: John G
authors.initials: JG
authors.lastname: Rose
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Lucarelli
authors.forename: Bradley N
authors.initials: BN
authors.lastname: Lemke
authors.forename: Richard K
authors.initials: RK
authors.lastname: Dortzbach
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Boxrud
authors.forename: Suzan
authors.initials: S
authors.lastname: Obagi
authors.forename: Sarit
authors.initials: S
authors.lastname: Patel
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000637:transplantation; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology* / Q000637:transplantation; D002452:Cell Count; D002469:Cell Separation; D002470:Cell Survival; D002498:Centrifugation; D031521:Fractionation, Field Flow; D006801:Humans; D013048:Specimen Handling; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014182:Transplantation, Autologous