A proteomic study on human osteoblastic cells proliferation and differentiation.
Changes in expression profiles for 17 proteins were ascertained in human mature osteoblasts compared to pre-osteoblasts (differentiation markers). A differential approach was used to highlight proteomic changes between human osteosarcoma cells and mature osteoblasts, showing a relative over-expression of 8 proteins (proliferation and tumor indicators), as well as under-expression of proteins also found down-regulated in pre-osteoblasts (specific markers of osteoblast differentiation). Our findings confirmed the differences between cell lines and primary human cell cultures and suggested caution on the use of osteosarcoma to study anti-osteoporotic drugs in humans.
Spreafico
Adriano
A
Dipartimento di Medicina Clinica e Scienze Immunologiche, Policlinico Le Scotte, Università degli Studi di Siena, Italy.
Frediani
Bruno
B
Capperucci
Caterina
C
Chellini
Francesca
F
Paffetti
Alessandro
A
D'Ambrosio
Chiara
C
Bernardini
Giulia
G
Mini
Roberta
R
Collodel
Giulia
G
Scaloni
Andrea
A
Marcolongo
Roberto
R
Santucci
Annalisa
A
D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D001842:Bone and Bones / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D012516:Osteosarcoma / Q000473:pathology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
D014408:Biomarkers, Tumor; D050659:Core Binding Factor Alpha 1 Subunit; D020543:Proteome; D015675:Osteocalcin; D000469:Alkaline Phosphatase