title: Glycerolipids as selective thrombin inhibitors from the fungus Stereum hirsutum.
abstract: A dry extract of Stereum hirsutum exhibited a significant inhibitory activity on thrombin (34%). A bioassay oriented fractionation of the extract of Stereum hirsutum has led to the isolation of two active fractions. On the basis of spectroscopic data, chemical reactions, and GC-MS analysis, complex mixtures of diacylglycerophospholipids (DAGPs) and diacylglycerols (DGs) have been isolated and identified. Docking studies suggest that some isolated compounds could bind to the thrombin active site in a similar manner as previously reported phosphonate thrombin inhibitors.
authors.affiliation: Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia.
authors.forename: Bojan
authors.initials: B
authors.lastname: Doljak
authors.forename: Francesca
authors.initials: F
authors.lastname: Cateni
authors.forename: Marko
authors.initials: M
authors.lastname: Anderluh
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Procida
authors.forename: Jelena
authors.initials: J
authors.lastname: Zilic
authors.forename: Marina
authors.initials: M
authors.lastname: Zacchigna
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D004075:Diglycerides / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005990:Glycerol / Q000737:chemistry*; D020404:Glycerophospholipids / Q000737:chemistry; D008024:Ligands; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D012015:Reference Standards; D013917:Thrombin / Q000037:antagonists & inhibitors*
chemical_list: D004075:Diglycerides; D005227:Fatty Acids; D020404:Glycerophospholipids; D008024:Ligands; D008055:Lipids; D013917:Thrombin; D005990:Glycerol