title: Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection.
abstract: A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.
authors.affiliation: Institute Pasteur São Paulo, São Paulo, SP, Brazil. pecarniel@yahoo.com.br
authors.forename: Pedro
authors.initials: P
authors.lastname: Carnieli Junior
authors.forename: Armando Moraes
authors.initials: AM
authors.lastname: Ventura
authors.forename: Edison Luiz
authors.initials: EL
authors.lastname: Durigon
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D002685:Chiroptera; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D004076:Digoxigenin; D004285:Dogs; D005814:Genes, Viral / Q000235:genetics*; D006736:Horses; D006801:Humans; D017403:In Situ Hybridization; D008163:Luminescent Measurements; D009698:Nucleoproteins / Q000235:genetics*; D011818:Rabies / Q000175:diagnosis* / Q000821:virology; D011820:Rabies virus / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012756:Sheep
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D009698:Nucleoproteins; D004076:Digoxigenin