Absence of beta-catenin alteration in hepatic tumors induced by p-nitroanisole in Crj:BDF1 mice.
In the present study, beta-catenin localization in hepatocellular neoplasms and hepatoblastomas, induced by oral administration of p-Nitroanisole (pNA) in Crj:BDF1 for 2 years, was evaluated by immunohistochemistry along with genetic alterations in exon 2 of beta-catenin by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) approach. Genomic DNA was isolated from paraffin sections of a total of 53 liver tumors. Immunohistochemical analysis revealed no abnormal accumulation of the beta-catenin protein in any of the cases. No mutations (0/13), 20% silent mutations (3/15) and 8% silent plus 12% functional mutations (2 + 3/25), not in the multiple phosphorylation sites of beta-catenin, were observed in hepatocellular adenomas, carcinomas and hepatoblastomas, respectively. The results indicate that beta-catenin does not play an important role in development of hepatic tumors induced by pNA in Crj:BDF1 mice.
Kushida
Masahiko
M
Department of Pathology, Osaka City University Medical School, Abeno-ku, Osaka 545-8585, Japan.
Aiso
Sigetoshi
S
Morimura
Keiichirou
K
Wei
Min
M
Wanibuchi
Hideki
H
Nagano
Kasuke
K
Fukushima
Shoji
S
D000818:Animals; D000840:Anisoles / Q000633:toxicity*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D007150:Immunohistochemistry; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D051379:Mice; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism*
D000840:Anisoles; D004273:DNA, Neoplasm; D051176:beta Catenin; C005306:4-nitroanisole