Regulation on maturation and function of dendritic cells by Astragalus mongholicus polysaccharides.
Astragalus mongholicus polysaccharides(ASP) isolated from one of the Chinese herbs-A. mongholicus which are known to have a variety of immunomodulatory activity. However, little is known about their immunomodulatory effects on murine bone marrow (BM)-derived dendritic cells (DC). DC are professional antigen presenting cells, which are pivotal for initiation of primary immune response. In this study, the regulatory effects of ASP on maturation and function of cultured murine BM-derived DC were investigated in vitro. ASP (10, 50, 100, 250 microg/ml) could increase the co-expression of CD-11c and MHC class II molecules on DC surface, and the 100 microg/ml is the optimal dose. The ability of unstimulated DC to uptake FITC-dextran was higher than that of ASP- or LPS-treated DC. We analyzed the concentration of IL-12 secreted by DC using ELISA. ASP-treated DC secreted a higher level of IL-12 than untreated DC. And ASP- or LPS-treated DC displayed a more mature morphology, with long protrusions, while untreated-DC displayed shorter protrusions than stimulated DC.
Shao
Peng
P
Institute of Molecular Biology and Biochemistry, School of Medicine, Zhejiang University Hangzhou 310006, People's Republic of China.
Zhao
Lu-Hang
LH
Zhi-Chen
Pan
Jian-Ping
JP
D000818:Animals; D027883:Astragalus Plant / Q000737:chemistry*; D039521:CD11c Antigen / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D011134:Polysaccharides / Q000494:pharmacology*
D039521:CD11c Antigen; D004365:Drugs, Chinese Herbal; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D011134:Polysaccharides; D018664:Interleukin-12