title: Improvement of the glaB promoter expressed in solid-state fermentation (SSF) of Aspergillus oryzae.
abstract: The glucoamylase-encoding gene (glaB) promoter should be very useful for recombinant protein production in solid-state fermentation (SSF) of Aspergillus oryzae. A 97-bp fragment containing the cis-element of the glaB promoter was inserted into the glaA promoter, which was little expressed in SSF. The chimeric promoter showed about a 24-fold increase in promoter activity in SSF. Eight copies of the 97-bp fragment were tandemly fused with the glaB promoter. The improved promoter showed about a 4.6-fold increase in promoter activity in SSF. The glaB gene was overexpressed under control of the improved glaB promoter in SSF. Recombinant glucoamylase production reached about 1524 mg/kg-broth for 2 d. The improved glaB promoter should be very useful for overproduction of a recombinant protein in SSF of A. oryzae.
authors.affiliation: Research Institute, Gekkeikan Sake Co., Ltd, Kyoto, Japan. h_ishida@gekkeikan.co.jp
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ishida
authors.forename: Yoji
authors.initials: Y
authors.lastname: Hata
authors.forename: Akitsugu
authors.initials: A
authors.lastname: Kawato
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Abe
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis* / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D005087:Glucan 1,4-alpha-Glucosidase