Improvement of the glaB promoter expressed in solid-state fermentation (SSF) of Aspergillus oryzae.
The glucoamylase-encoding gene (glaB) promoter should be very useful for recombinant protein production in solid-state fermentation (SSF) of Aspergillus oryzae. A 97-bp fragment containing the cis-element of the glaB promoter was inserted into the glaA promoter, which was little expressed in SSF. The chimeric promoter showed about a 24-fold increase in promoter activity in SSF. Eight copies of the 97-bp fragment were tandemly fused with the glaB promoter. The improved promoter showed about a 4.6-fold increase in promoter activity in SSF. The glaB gene was overexpressed under control of the improved glaB promoter in SSF. Recombinant glucoamylase production reached about 1524 mg/kg-broth for 2 d. The improved glaB promoter should be very useful for overproduction of a recombinant protein in SSF of A. oryzae.
Ishida
Hiroki
H
Research Institute, Gekkeikan Sake Co., Ltd, Kyoto, Japan. h_ishida@gekkeikan.co.jp
Hata
Yoji
Y
Kawato
Akitsugu
A
Abe
Yasuhisa
Y
D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis* / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D005087:Glucan 1,4-alpha-Glucosidase