title: JSAP1 is required for the cell adhesion and spreading of mouse embryonic fibroblasts.
abstract: The roles of JSAP1 and JIP1 in cell adhesion and spreading were examined using mouse embryonic fibroblasts (MEFs) deficient in JIP1 (JIP1-KO), JSAP1 (JSAP1-KO), and in both JIP1 and JSAP1 (double-KO), and by using their wild type. After being plated on fibronectin-coated culture plates, wild type MEFs rapidly adhered and differentiated to typical longitudinal fibroblasts in 4 h. JSAP1-KO MEFs showed a similar sequence of adhesion and cell spreading, but their adhesion was weak, and cell spreading sequence proceeded in a delayed manner compared with the wild type. In spreading JSAP1-KO MEFs, adhesion-triggered actin cytoskeleton reorganization and FAK activation proceeded at a slower pace than in wild type MEFs. The cellular properties of double-KO MEFs and JIP1-KO MEFs were similar to those of JSAP1-KO MEFs and wild type MEFs, respectively. These results suggest that JSAP1 plays a role in adhesion and cell spreading by regulating the rapid reorganization of the actin cytoskeleton.
authors.affiliation: Division of Nano Sciences and Ewha Institute of Neuroscience, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Chae
authors.forename: Hye-Yeong
authors.initials: HY
authors.lastname: Ha
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Im
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Sungmi
authors.initials: S
authors.lastname: Park
authors.forename: Pyung-Lim
authors.initials: PL
authors.lastname: Han
mesh_terms: D000199:Actins / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion / Q000502:physiology*; D003599:Cytoskeleton / Q000502:physiology; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000502:physiology*; D013997:Time Factors
chemical_list: D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; C403619:Mapk8ip3 protein, mouse; D009419:Nerve Tissue Proteins