[Determination of acetylharpagide in Ajuga decumbens by HPLC].
OBJECTIVE
To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens.


METHODS
The chromatographic conditions were as follows: a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (15:85), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm.


RESULTS
Linearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively.


CONCLUSIONS
The contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.
Yue
Dang-Kun
DK
Institue of Chinese Materia Medica, China Academy of Chinese Medicial Sciences, Bejing 100700, China.
Ding
Guang-Zhi
GZ
Wang
Wei-Hao
WH
Wang
Zhi-Min
ZM
Li
Jian-Rong
JR
Tong
Yan
Y
D031328:Ajuga / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011714:Pyrans / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004365:Drugs, Chinese Herbal; D011714:Pyrans; C077626:8-O-acetylharpagide