Human embryonic stem cells as a cellular model for human disorders.
Human embryonic stem cells (HESCs) are pluripotent cell lines derived from the inner cell mass (ICM) of embryos at the blastocyst stage. These cells possess self renewal capacity and differentiation potential to all three embryonic germ layers. These unique characters made HESCs an attractive research tool for studying early human developmental processes as well as a potential therapeutic tool for various human diseases. Here, we focus on HESCs as a cellular model for human disorders. The advantages of such models as well as the various methodologies to achieve HESCs carrying a genetic defect will be discussed.
Friedrich Ben-Nun
Inbar
I
Department of Genetics, Life Science Institute, The Hebrew University, Jerusalem 91904, Israel.
Benvenisty
Nissim
N
D000818:Animals; D002353:Carrier State; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D004700:Endocrine System Diseases / Q000235:genetics / Q000503:physiopathology*; D030342:Genetic Diseases, Inborn / Q000235:genetics / Q000503:physiopathology*; D005821:Genetic Techniques; D006801:Humans; D008954:Models, Biological; D034622:RNA Interference; D011995:Recombination, Genetic; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D014162:Transfection