The effect of natural extracellular matrix deposited on synthetic polymers on cultured primary hepatocytes.
It is well known that natural extracellular matrix (ECM) molecules are deposited on the surface of biomaterials during culture of cells and affect cellular behaviors. However, it has not been fully understood what kinds of ECM molecules are deposited on the surface of biomaterials although the cellular behaviors were affected by deposited ECM. In this study, to investigate the effect of deposited natural ECM on behaviors of hepatocytes cultured on biomaterials such as poly (N-p-vinylbenzyl-4-O-beta-D-galactopyranosyl-D-gluconamide) (PVLA) as a hepatocyte-specific matrix and poly (L-lysine) (PLL) as a non-specific one during the culture of hepatocytes in vitro, we investigated expression pattern of ECM genes and adsorption of ECM molecules onto PVLA- and PLL-coated surfaces. It was found that the expression levels of type I collagen and fibronectin genes in the hepatocytes cultured on PVLA-coated surface were different from them in the hepatocytes cultured on PLL-coated one. Also, the results showed that laminin was dominantly deposited on PVLA-coated surface whereas fibronectin was dominantly deposited on PLL-coated one. Hepatocytes maintained liver-specific functions on PVLA- and laminin-coated surfaces. It is thought that deposited laminin during the culture of hepatocytes affects the liver-specific functions of hepatocytes cultured on PVLA-coated surface.
Hoshiba
Takashi
T
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Cho
Chong Su
CS
Murakawa
Akiko
A
Okahata
Yoshio
Y
Akaike
Toshihiro
T
D000199:Actins / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004187:Disaccharides / Q000737:chemistry*; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007797:Laminin / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000502:physiology; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties; D014753:Vinyl Compounds / Q000737:chemistry*
D000199:Actins; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D004187:Disaccharides; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D011108:Polymers; D014753:Vinyl Compounds; C427524:poly-(N-p-vinylbenzyl-O-galactopyranosyl-(1-4)-gluconamide); D011107:Polylysine