Rat hepatocyte cultures: collagen gel sandwich and immobilization cultures.
Mimicking the in vivo microenvironment is one of the current strategies to maintain liver-specific functionality in primary cultured hepatocytes for long periods. Freshly isolated hepatocytes entrapped in collagen gel type I (collagen gel immobilization culture) or sandwiched between two layers of hydrated collagen type I (collagen gel sandwich culture) are known to display liver-specific functions (e.g., biotransformation capacity) for more than 6 wk. We describe how to set up both types of organotypical hepatocyte culture systems. Besides a detailed protocol, we give some practical tips, taken from our own experience with long-term hepatocyte culture.
Vinken
Mathieu
M
Department of Toxicology, Vrije Universiteit Brussel, Belgium.
Elaut
Greetje
G
Henkens
Tom
T
Papeleu
Peggy
P
Snykers
Sarah
S
Vanhaecke
Tamara
T
Rogiers
Vera
V
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D051381:Rats
D003094:Collagen