[Differential gene expression in the jellyfish Aurelia aurita].
The body of Aurelia aurita, as well as other diploblasts, consists of two epithelial layers: ectodermal and gastral epithelium. These two tissues are separated by mesoglea, or extracellular matrix. In most coelenterates mesoglea is acellular. In A. aurita mesogleal cells are scattered in mesoglea. Differential display PCR was used to compare mRNA pools from ectodermal epithelium, gastral epithelium and mesoglea. 4 novel gene fragments were cloned and sequenced. According to RTPCR results, one of these fragments is differentially expressed in the ectodermal epithelium.
Matveev
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D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D004847:Epithelial Cells / Q000502:physiology; D015870:Gene Expression / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D037401:Scyphozoa / Q000166:cytology / Q000235:genetics*
D012333:RNA, Messenger