Roots and leaves display contrasting oxidative response during salt stress and recovery in cowpea.
In this study, we compare some antioxidative responses of leaves and roots associated to growth reduction in cowpea plants (Vigna unguiculata) during short-term salt stress and recovery. The salt treatment was imposed (200 mM NaCl) for six consecutive days and the salt withdrawal after 3 d. The salt treatment caused an almost complete cessation in the relative growth rate of both leaves and roots. Although NaCl withdrawal has induced an intense reduction in the Na(+) content from the leaves and roots, the growth recovery was slight, after 3 d. The leaf lipid peroxidation was increased in salt-stressed plants and slightly reduced in recovered plants after 3 d. Surprisingly, in the salt-stressed roots it decreased markedly after 3 d treatment and in the pre-stressed/recovered roots it was restored to levels near to the control. In leaves, catalase (CAT) activity showed a rapid and prominent decrease after 1 d of NaCl treatment and salt withdrawal had no effect on its recovery. In contrast, the root CAT activity was not changed by effects of both NaCl and salt withdrawal, over time interval. Leaf superoxide dismutase (SOD) activity did not change in all treatments, whereas in roots it significantly decreased after 3 d of salt treatment and recovered after NaCl withdrawal. Contrasting to the other enzymes, the guaiacol-peroxidase activity increased in leaves and roots, reaching almost 200% of control values and it significantly decreased in both organs from the pre-stressed/recovered plants. In conclusion, cowpea roots and leaves present distinct mechanisms of response to lipid peroxidation and CAT and SOD activities during salt stress and recovery. However, these responses and/or the oxidative damages caused by reactive oxygen species were not related with the growth reduction.
Cavalcanti
Fabio Rossi
FR
Laboratório de Metabolismo de Plantas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, CP 6033, CEP 60451-970, Fortaleza, Ceará, Brazil.
Lima
João Paulo Matos Santos
JP
Ferreira-Silva
Sérgio Luiz
SL
Viégas
Ricardo Almeida
RA
Silveira
Joaquim Albenisio Gomes
JA
D007887:Fabaceae / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D018515:Plant Leaves / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors
D012965:Sodium Chloride