title: Application of in situ ductal perfusion to facilitate isolation of high-quality RNA from mouse pancreas.
abstract: A technique to isolate high-quality intact RNA from murine pancreas is described. This technique involves in situ ductal perfusion of the pancreas with an RNase inhibitor prior to removal of the organ for RNA extraction. In this way, the pancreatic RNases are inhibited in situ allowing good yields of intact RNA, suitable for studies on pancreatic gene transcription by real-time PCR or microarray analysis, to be obtained in a reliable way.
authors.affiliation: University of British Columbia, Vancouver and Child and Family Research Institute, Vancouver, BC, Canada.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Mullin
authors.forename: Galina
authors.initials: G
authors.lastname: Soukatcheva
authors.forename: C Bruce
authors.initials: CB
authors.lastname: Verchere
authors.forename: Janet K
authors.initials: JK
authors.lastname: Chantler
mesh_terms: D000818:Animals; D051379:Mice; D008815:Mice, Inbred Strains; D008953:Models, Anatomic; D010179:Pancreas / Q000737:chemistry* / Q000601:surgery; D010183:Pancreatic Ducts / Q000502:physiology*; D010477:Perfusion / Q000379:methods*; D012313:RNA / Q000302:isolation & purification*; D012260:Ribonucleases / Q000037:antagonists & inhibitors*
chemical_list: D012313:RNA; D012260:Ribonucleases