The polymerase chain reaction and its application to clinical plastic surgery.
Molecular biology has become an essential component in many fields of modern medical research, including plastic surgery. Research into the molecular mechanisms underlying many disease processes offer increased understanding of the pathogenesis of disease and provide exciting therapeutic possibilities. Yet for many clinicians, the presentation of much research into molecular biological processes is couched in confusing terminology and based on scientific techniques, the basis of which are frequently difficult for the clinician to understand. The purpose of this review is to present an introduction to some of the molecular biological techniques currently in use, namely the polymerase chain reaction (PCR) and explore its applications to different aspects of plastic surgery. This review explores the role PCR now plays in all aspects of modern plastic surgery practise, with particular emphasis on normal and abnormal wound healing, the diagnosis of craniofacial anomalies, the diagnosis and treatment of cancer including melanoma and squamous cell carcinoma of the head and neck, and burns.
Rea
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Department of Plastic and Reconstructive Surgery, Cork University Hospital Wilton, Cork, Ireland. suzanne.rea@health.wa.gov.au
O'Sullivan
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D002056:Burns / Q000276:immunology; D019465:Craniofacial Abnormalities / Q000235:genetics; D006258:Head and Neck Neoplasms / Q000601:surgery; D006801:Humans; D008545:Melanoma / Q000175:diagnosis; D016133:Polymerase Chain Reaction / Q000379:methods*; D019651:Plastic Surgery Procedures / Q000379:methods*; D012878:Skin Neoplasms / Q000175:diagnosis; D014945:Wound Healing / Q000235:genetics