Wound healing effect of silk fibroin/alginate-blended sponge in full thickness skin defect of rat.
Silk fibroin (SF) and alginate (AA) have been proved to be invaluable natural materials in the field of biomedical engineering. This study was designed to compare the wound healing effect of SF, AA and SF/AA-blended sponge (SF/AA) with clinically used Nu Gauze(TM) (CONT) in a rat full thickness wound model. Two circular skin wounds on the back of rat were covered with either of CONT, SF, AA or SF/AA. On the postoperative days of 3, 7, 10 and 14, residual wound area was calculated, and skin wound tissues were biopsied to measure the area of regenerated epithelium and collagen deposition as well as the number of proliferating cell nuclear antigen (PCNA)-immunoreactive cells. Half healing time (HT(50)) of SF/AA was dramatically reduced as compared with that of SF, AA or CONT. Furthermore, SF/AA significantly increased the size of re-epithelialization and the number of PCNA positive cells, whereas the effect of SF/AA on collagen deposition was not significantly different as compared with that of SF or AA. These results demonstrate that the wound healing effect of SF/AA is the best among other treatments including SF and AA, and this synergic effect is mediated by re-epithelialization via rapid proliferation of epithelial cell.
Roh
Dae-Hyun
DH
Department of Veterinary Physiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, 151-742, South Korea.
Kang
Seuk-Yun
SY
Kim
Jeom-Yong
JY
Kwon
Young-Bae
YB
Young Kweon
Hae
H
Lee
Kwang-Gill
KG
Park
Young-Hwan
YH
Baek
Rong-Min
RM
Heo
Chan-Yeong
CY
Choe
Joon
J
Lee
Jang-Hern
JH
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D001684:Biological Dressings; D004848:Epithelium / Q000187:drug effects; D005349:Fibroins / Q000494:pharmacology*; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D047011:Silk; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
D000464:Alginates; D006603:Hexuronic Acids; D018809:Proliferating Cell Nuclear Antigen; D047011:Silk; D020723:Glucuronic Acid; D005349:Fibroins