title: Optimization of HPLC chromatographic conditions for determination of Transkarbam 12 and its degradation products.
abstract: This paper deals with searching of HPLC chromatographic conditions for determination and separation of Transkarbam 12 (T 12) and its two main degradation products (omega-aminocaproic acid and dodecylalcohol). T 12 is a new substance which belongs to the group of accelerators of transdermal penetration. Chromatographic separation was achieved using Separon SGX C18 analytical column (150 mm x 3 mm i.d.; 5 microm). Mobile phase contained acetonitrile and sodium acetate buffer pH 4.5 at the flow rate of 1 ml/min. Separation was carried out under the conditions of gradient elution. After the modification of the structure by derivatization reagent (3,5-dinitrobenzoyl chloride) detection at wavelength 230 nm was realized. The aim of this study was not only the optimization of the separation of derivatization reagent and derivatized T 12, Ak and D but also optimal derivatization processes for all three substances.
authors.affiliation: Department of Pharmaceutical Chemistry and Drug Control, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic. ivana.pasakova@faf.cuni.cz
authors.forename: I
authors.initials: I
authors.lastname: Pasáková
authors.forename: J
authors.initials: J
authors.lastname: Klimes
authors.forename: J
authors.initials: J
authors.lastname: Sochor
authors.forename: A
authors.initials: A
authors.lastname: Hrabálek
mesh_terms: D000614:Aminocaproates / Q000032:analysis; D002219:Carbamates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007851:Dodecanol / Q000032:analysis
chemical_list: D000614:Aminocaproates; D002219:Carbamates; C492029:transkarbam 12; D007851:Dodecanol