title: Identification of major proteins secreted by Cryptococcus neoformans.
abstract: The characterization of proteins secreted by Cryptococcus neoformans is of relevance to the identification of vaccine candidates, because concentrated supernatants from the fungus have been shown to be immunoprotective in previous studies. After fractionation of supernatants by anion exchange chromatography and preparative electrophoresis, we obtained the N-terminal amino acid sequences of 13 major proteins. Using a C. neoformans nucleotide database, we were able to clone and sequence the ORFs coding for 12 of these proteins. Some of the genes are identical to previously described ones, while six encode novel proteins, including four putative mannoproteins. The molecular characterization of these and other secreted products may provide useful information in the development of immune-based strategies to control cryptococcosis.
authors.affiliation: Department of Pathology and Experimental Microbiology, University of Messina, Messina, Italy.
authors.forename: Carmelo
authors.initials: C
authors.lastname: Biondo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Mancuso
authors.forename: Angelina
authors.initials: A
authors.lastname: Midiri
authors.forename: Mauro
authors.initials: M
authors.lastname: Bombaci
authors.forename: Luciano
authors.initials: L
authors.lastname: Messina
authors.forename: Concetta
authors.initials: C
authors.lastname: Beninati
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Teti
mesh_terms: D000595:Amino Acid Sequence; D003455:Cryptococcus neoformans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data
chemical_list: D017077:Culture Media, Conditioned; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C025363:mannoproteins