Ultrastructural changes of nucleoli in common wheat induced by actinomycin D.
Common wheat root tip meristematic cells were treated with low concentrations of actinomycin D (ActD), then stained whole by silver nitrate. We showed by transmission electron microscopy that the typical nucleolar structure did not form, but a granular and fibrillar network was exhibited in the nucleolar region. Our results support a correlation between nucleolar organization/assembly and the activation of RNA Polymerase I transcription. Furthermore, we speculate that the fibrillar network present in the nucleolar region of ActD treated cells may represent the basic skeletal structure required to support the nucleolus.
Dai
J
J
College of Life Science, Capital Normal University, Beijing, 100037, People's Republic of China.
Han
Y
Y
Xu
B
B
Li
Y
Y
Liu
J
J
Zhao
Y
Y
Zhang
F
F
D002466:Cell Nucleolus / Q000187:drug effects* / Q000648:ultrastructure*; D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018519:Meristem / Q000648:ultrastructure; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D018517:Plant Roots / Q000166:cytology; D014908:Triticum / Q000648:ultrastructure*
D019384:Nucleic Acid Synthesis Inhibitors; D003609:Dactinomycin