[Preparation of solid lipid nanoparticles loaded with Xionggui powder-supercritical carbon dioxide fluid extraction and their evaluation in vitro release].
OBJECTIVE
To study the preparation of solid lipid nanoparticles loaded with Xionggui powder-supercritical carbon dioxide fluid extraction and their evaluation in vitro release.


METHODS
To prepare solid lipid nanoparticles (SLN) loaded with Xionggui powder-supercritical carbon dioxide fluid extraction (XG-CO2-SFE) using a hot dispersion- ultrasonic technique, establishing the best prescription of XG-CO2-SFE-SLN through orthogonal design methods using entrapment efficiency of nanoparticles as index, and investigating their physicochemical characterizations. The invro release action of SLN was studied in different dissolution mediums using dynamic dialyse method.


RESULTS
The best prescription was: phospholipid: F-68: stearie acid glyceride = 5: 2 : 1, the entrapment efficiency of nanoparticles was 96.3%, and the results revealed the nanoparticles were sphere like with the mean size of 245.8 nm, the mean Zeta potential was -33.5 mV. The in vitro release meet to Weibull distribution in physiological brine and to single-index model in pH 7.4 phosphate liquid (40% EtOH).


CONCLUSIONS
The preparation method of the XG-CO2-SFE-SLN was appropriate, and the XG-CO2-SFE-SLN was released completely.
Chen
Yan-jun
YJ
Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing. ivycyj@163.com
Jin
Ri-xian
RX
Zhou
Ya-qin
YQ
Zeng
Jing
J
Zhang
He
H
Feng
Qing-ran
QR
D029971:Angelica sinensis / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004338:Drug Combinations; D004339:Drug Compounding / Q000379:methods*; D016503:Drug Delivery Systems; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000302:isolation & purification; D030004:Ligusticum / Q000737:chemistry*; D049329:Nanostructures; D010316:Particle Size; D010743:Phospholipids; D020442:Poloxamer; D012995:Solubility
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D010743:Phospholipids; D020442:Poloxamer