Identification of pseudo attP sites for phage phiC31 integrase in bovine genome.
Streptomyces phage phiC31 integrase was found to mediate site-specific integration of foreign genes at pseudo attP sites of genomes in human, mouse, rat, and Drosophila. This paper reports that phiC31 integrase can also mediate homologous recombination between attB and pseudo attP sites in bovine cells and foreign gene integration was increased at least 2-fold in bovine fibroblasts or Madin-Darby bovine kidney (MDBK) cells. Two intrinsic pseudo attP sites named BpsF1 and BpsM1 located in the inter-gene regions on chromosome 28 and 19, respectively, were identified in bovine genome. These pseudo attP sites shared similar characteristics with those from other species as previously described. Our study demonstrated that the phiC31 integrase system provides a new potential for genetic engineering of the bovine genome and might be beneficial for the research on ruminant.
Ma
Qing-wen
QW
Shanghai Institute of Medical Genetics, Shanghai Jiao Tong University, 24/1400 West Beijing Road, Shanghai 200040, PR China.
Sheng
Hai-qing
HQ
Yan
Jing-bin
JB
Cheng
Shan
S
Huang
Ying
Y
Chen-Tsai
Yanru
Y
Ren
Zhao-rui
ZR
Huang
Shu-zhen
SZ
Zeng
Yi-tao
YT
D000818:Animals; D001287:Attachment Sites, Microbiological; D001665:Binding Sites; D002417:Cattle; D002460:Cell Line; D002874:Chromosome Mapping; D004285:Dogs; D016678:Genome; D019426:Integrases / Q000235:genetics*; D011995:Recombination, Genetic; D013302:Streptomyces / Q000821:virology*; D016662:Virus Integration
D019426:Integrases