title: [Preparation of solid lipid nanoparticles loaded with Xionggui powder-supercritical carbon dioxide fluid extraction and their evaluation in vitro release].
abstract: OBJECTIVE
  To study the preparation of solid lipid nanoparticles loaded with Xionggui powder-supercritical carbon dioxide fluid extraction and their evaluation in vitro release.
  
  
  METHODS
  To prepare solid lipid nanoparticles (SLN) loaded with Xionggui powder-supercritical carbon dioxide fluid extraction (XG-CO2-SFE) using a hot dispersion- ultrasonic technique, establishing the best prescription of XG-CO2-SFE-SLN through orthogonal design methods using entrapment efficiency of nanoparticles as index, and investigating their physicochemical characterizations. The invro release action of SLN was studied in different dissolution mediums using dynamic dialyse method.
  
  
  RESULTS
  The best prescription was: phospholipid: F-68: stearie acid glyceride = 5: 2 : 1, the entrapment efficiency of nanoparticles was 96.3%, and the results revealed the nanoparticles were sphere like with the mean size of 245.8 nm, the mean Zeta potential was -33.5 mV. The in vitro release meet to Weibull distribution in physiological brine and to single-index model in pH 7.4 phosphate liquid (40% EtOH).
  
  
  CONCLUSIONS
  The preparation method of the XG-CO2-SFE-SLN was appropriate, and the XG-CO2-SFE-SLN was released completely.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing. ivycyj@163.com
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Chen
authors.forename: Ri-xian
authors.initials: RX
authors.lastname: Jin
authors.forename: Ya-qin
authors.initials: YQ
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Zeng
authors.forename: He
authors.initials: H
authors.lastname: Zhang
authors.forename: Qing-ran
authors.initials: QR
authors.lastname: Feng
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004338:Drug Combinations; D004339:Drug Compounding / Q000379:methods*; D016503:Drug Delivery Systems; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000302:isolation & purification; D030004:Ligusticum / Q000737:chemistry*; D049329:Nanostructures; D010316:Particle Size; D010743:Phospholipids; D020442:Poloxamer; D012995:Solubility
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D010743:Phospholipids; D020442:Poloxamer