Purification, and properties of a bovine uricase.
Uricase from bovine kidney, purified to homogeneity level, had a molecular weight of 70 kDa. The apparent K(m) and V(max) values for uric acid hydrolysis were 0.125 mM and 102 IU mg(-1) protein respectively. The activation energy requirement for uric acid hydrolysis by uricase and inactivation of enzyme were 11.6 and 14.5 kJ/M respectively. Both enthalpy (Delta H*) and entropy of activation (Delta S*) for uricase activity were lower than those reported for some thermostable enzymes.
Rajoka
Muhammad Ibrahim
MI
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. mirajoka@nibge.org
Rehman
Khalil-Ur-
KU
Mehraj
Munazza
M
Akhtar
Muhammad Waheed
MW
Zia
Muhammad Anjum
MA
D000818:Animals; D002417:Cattle; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008970:Molecular Weight; D013696:Temperature; D013816:Thermodynamics; D014503:Urate Oxidase / Q000302:isolation & purification* / Q000378:metabolism
D014503:Urate Oxidase