Effects of taohong siwu decoction II in the chick chorioallantoic membrane (CAM) assay and on B16 melanoma in mice and endothelial cells ECV304 proliferation.
OBJECTIVE
To investigate the anti-angiogenesis action of Taohong Siwu Decoction II (THSWD II).


METHODS
The chick chorioallantoic membrane (CAM) assay was adopted to study the anti-angiogenesis action of THSWD II; the MTT test was used to investigate its effect on proliferation of the human umbilical vein endothelial cells ECV304; and the immunohistochemical method was used to observe the effect of THSWD II on the expression of kinase insert domain containing receptor/fetal liver kinase 1 (KDR/Flk-1) and the microvessel density (MVD) of B16 melanoma in mice.


RESULTS
After treatment with THSWD II, the blood vessel index of CAM and the absorbency of ECV304 in the THSWD II 1 mg/ml group and the 2 mg/ml group decreased significantly (P < 0.01); the weight, the expression of KDR/Flk-1 and the MVD of B16 melanoma in mice reduced significantly in the THSWD II 5 g/kg group, the 10 g/kg group and the TSHSWD 10g/kg plus cyclophosphamide group (P < 0.01).


CONCLUSIONS
THSWD II has the actions of anti-angiogenesis, and inhibiting the proliferation of ECV304 cells and the growth of B16 melanoma. The clinical anti-tumour mechanism is considered to berelated possibly to its anti-angiogenesis action by inhibiting the expression of KDR/FIK-1.
Xu
Xiaoyu
X
College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400050, China.
Wang
Shumei
S
Chen
Weihai
W
Chen
Gang
G
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004531:Eggs; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D008546:Melanoma, Experimental / Q000098:blood supply* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis / Q000235:genetics
D020533:Angiogenesis Inhibitors; D004365:Drugs, Chinese Herbal; D040301:Vascular Endothelial Growth Factor Receptor-2