Possible activation of murine T lymphocyte through CD98 is independent of interleukin 2/interleukin 2 receptor system.
CD98 is a widely expressed cell surface heterodimeric protein of 125 kDa. Its expression is upregulated during lymphocyte activation induced by mitogen, superantigen, conventional antigen, and a combination of phorbol myristate acetate (PMA) and ionomycin. However, the role of CD98 in the immune system is not so well understood. The role of CD98 in murine T lymphocyte proliferation was investigated, especially in correlation with the interleukin 2 (IL-2)/interleukin 2 receptor (IL-2R) system. Monoclonal antibody (mAb) directed against murine CD98 heavy chain (mCD98HC) suppressed the proliferation of lymphocytes stimulated with concanavalin A (Con A). Anti-mCD98HC mAb did not suppress the expression of IL-2Ralpha. Anti-IL-2Ralpha mAb, which suppressed DNA synthesis, did not inhibit the expression of CD98HC. Murine IL-2 (recombinant), which induced considerable DNA synthesis by lymphocytes stimulated with a sub-optimal dose of Con A, did not induce CD98HC expression in lymphocytes. In addition, anti-mCD98HC mAb did not inhibit the production of IL-2 by lymphocyte stimulated with Con A. Taken together with these findings, it was speculated that the CD98 system is independent of the IL-2/IL-2R system in murine T lymphocyte activation.
Komada
Hiroshi
H
Department of Microbiology, Mie University School of Medicine, Mie, Japan.
Imai
Akiyo
A
Hattori
Emi
E
Ito
Morihiro
M
Tsumura
Hideki
H
Onoda
Toshiko
T
Kuramochi
Mariko
M
Tani
Machiko
M
Yamamoto
Kanako
K
Yamane
Mizuho
M
Kawano
Mitsuo
M
Nishio
Machiko
M
Yuasa
Kimitaka
K
O'Brien
Myles
M
Yamamoto
Hidetaka
H
Uematsu
Jun
J
Tsurudome
Masato
M
Ito
Yasuhiko
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D027282:Fusion Regulatory Protein 1, Heavy Chain / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D000911:Antibodies, Monoclonal; D027282:Fusion Regulatory Protein 1, Heavy Chain; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D003208:Concanavalin A