[Development of tissue microarray technology (TMA) for immunohistochemical study of molecular expression profiling in prostate cancer (part 1)].
Tissue microarray technology (TMA) is nowadays considered as a powerful tool for the high-throughput analysis of molecular expression pattern of cancer. In this manuscript we show the experience of both groups in the design and building of a TMA for the study of protein expression pattern of prostatecancer as well as a summary of the technical points to analyze the results obtained with this technology. Today, different data generated by the immunostained tissues are studied to achieve a molecular profile in different clinical scenarios.
Lorente Garín
J A
JA
Servicio y Cátedra de Urología, Laboratorio de Uro-Patología Aplicada, Hospital del Mar, Barcelona. jlorente@imas.imim.es
Lloreta Trull
J
J
Allepuz Losa
C
C
Plaza Mas
L
L
Rioja Sanz
L A
LA
Gelabert Mas
A
A
D004867:Equipment Design; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D046228:Microarray Analysis / Q000295:instrumentation*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*