title: Organ-specific patterns of P450arom gene isoforms are modulated by p,p'-DDE in adult male European common frog, Rana temporaria.
abstract: The organ-specific gene expression patterns of P450arom isoforms have been studied in European common frog, Rana temporaria after exposure to DDE, using real-time PCR. Four groups of frogs were subcutaneously injected with DDE at 0.01, 0.1, 1 and 10 mg/kg body weight and one group, serving as the control was injected with pure corn oil. Brain, kidney, testis and liver P450aromA and P450aromB gene expressions were evaluated at day 14 after exposure. P450aromB data show that 0.1 and 10 mg DDE/kg doses caused 76% and 63% (testis) and 79% and 80% reductions, respectively, of mRNA levels. Brain P450aromB mRNA decreased 10% and 34%, respectively, after exposure to 0.01 and 0.1 mg DDE/kg. Thereafter, a 185% and 177% induction response of brain P450aromB was observed in the groups treated with 1 and 10 mg DDE/kg, respectively. In the kidney, 0.01, 0.1, 1 and 10 mg DDE/kg induced a 516%, 178%, 466% and 247% induction of P450aronB mRNA, respectively. P450aromA expression was not quantified in any of the organs. The relative abundance of P450aromB gene expression in different organs is in the order: kidney > brain > liver > testis. The present data suggest potential detrimental effect of organochlorine pesticides (OCs) and their metabolites on the European frog steroidogenic pathways. Given the high persistency in the environment and continued use in developing countries coupled with the tendency for global atmospheric transport, OCs and their metabolites such as DDE will remain a focus of concern both for scientific and societal reasons.
authors.affiliation: Department of Biology, Norwegian University of Science and Technology (NTNU), Høgskoleringen 5, 7491 Trondheim, Norway. arukwe@bio.ntnu.no
authors.forename: Augustine
authors.initials: A
authors.lastname: Arukwe
mesh_terms: D000818:Animals; D001141:Aromatase / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D017931:DNA Primers / Q000737:chemistry; D003633:Dichlorodiphenyl Dichloroethylene / Q000008:administration & dosage / Q000633:toxicity*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D007527:Isoenzymes / Q000737:chemistry / Q000187:drug effects / Q000235:genetics; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D011896:Rana temporaria / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000187:drug effects / Q000201:enzymology
chemical_list: D017931:DNA Primers; D007306:Insecticides; D007527:Isoenzymes; D003633:Dichlorodiphenyl Dichloroethylene; D001141:Aromatase