title: Retinol-encapsulated low molecular water-soluble chitosan nanoparticles.
abstract: This aim of this study was to encapsulate retinol into chitosan nanoparticles and reconstitute it into aqueous solution. Retinol-encapsulated chitosan nanoparticles were prepared for application of cosmetic and pharmaceutical applications. Retinol-encapsulated chitosan nanoparticle has a spherical shape and its particle sizes were around 50-200 nm according to the drug contents. Particle size was increased according to the increase of drug contents. Solubility of retinol is able to increase by encapsulation into chitosan nanoparticles more than 1600-fold. It was suggested that retinol was encapsulated into chitosan nanoparticles by ion complex as a result of FT-IR spectra. Specific peak of chitosan at 1590 cm(-1) was divided to semi-doublet due to the electrostatic interaction between amine group of chitosan and hydroxyl group of retinol. At (1)H NMR spectra, specific peaks of retinol disappeared when retinol-encapsulated chitosan nanoparticles were reconstituted into D(2)O while specific peaks both of retinol and chitosan appeared at D(2)O/DMSO (1/4, v/v) mixture. XRD patterns also showed that crystal peaks of retinol were disappeared by encapsulation into chitosan nanoparticles. Retinol-encapsulated nanoparticles were completely reconstituted into aqueous solution as same as original aqueous solution and zeta potential of reconstituted chitosan nanoparticles was similar to their original solution. At HPLC study, retinol was stably and efficiently encapsulated into chitosan nanoparticles.
authors.affiliation: Department of Polymer Science and Engineering, Sunchon National University, Jeonnam 540-742, Republic of Korea.
authors.forename: Dong-Gon
authors.initials: DG
authors.lastname: Kim
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Jeong
authors.forename: Changyong
authors.initials: C
authors.lastname: Choi
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Roh
authors.forename: Seong-Koo
authors.initials: SK
authors.lastname: Kang
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Jang
authors.forename: Jae-Woon
authors.initials: JW
authors.lastname: Nah
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004339:Drug Compounding; D004355:Drug Stability; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010316:Particle Size; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014801:Vitamin A / Q000737:chemistry*; D014815:Vitamins / Q000737:chemistry*; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D012997:Solvents; D014815:Vitamins; D014867:Water; D014801:Vitamin A; D048271:Chitosan