title: Survival and initial chromatid breakage in normal and tumour cells exposed in vitro to gamma rays and carbon ions at the HIRFL.
abstract: Human hepatoma and normal liver cells were irradiated with (12)C(6+) ion beams (linear energy transfer (LET) = 96 keV microm(-1)) and gamma-rays at the Heavy Ion Research Facility in Lanzhou (HIRFL). The numbers and types of chromatid breaks were detected using the premature chromosome condensation technique. Irradiation with (12)C(6+) ions produced a majority of isochromatid break types, while chromatid breaks were dominant for irradiation with gamma-rays. Experimental results showed that the initial level of chromatid breaks is clearly related to the absorbed dose from (12)C(6+) ions and gamma-rays. The (12)C(6+) ions are relatively more effective at inducing initial chromatid breaks when compared with the gamma-rays. A relative biological effectiveness (RBE) of about 2.5 resulted for the induction of initial chromatid breaks by (12)C(6+) ions relative to gamma-rays in both cell lines.
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences.
authors.forename: Y
authors.initials: Y
authors.lastname: Jianshe
authors.forename: L
authors.initials: L
authors.lastname: Wenjian
authors.forename: J
authors.initials: J
authors.lastname: Xiaodong
authors.forename: J
authors.initials: J
authors.lastname: Xigang
authors.forename: G
authors.initials: G
authors.lastname: Chuanling
authors.forename: W
authors.initials: W
authors.lastname: Wei
authors.forename: G
authors.initials: G
authors.lastname: Qingxiang
mesh_terms: D002244:Carbon / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000532:radiotherapy*; D002470:Cell Survival / Q000528:radiation effects; D002842:Chromatids / Q000528:radiation effects*; D019457:Chromosome Breakage; D005720:Gamma Rays; D020450:Heavy Ions; D022781:Hepatocytes / Q000528:radiation effects; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D014407:Tumor Cells, Cultured
chemical_list: D002244:Carbon