title: Characterisation and molecular epidemiology of extended-spectrum beta-lactamase-producing Enterobacter cloacae isolated from a district teaching hospital in Taiwan.
abstract: Enterobacter cloacae (n = 110) isolates from a district hospital in Taiwan were screened for extended-spectrum beta-lactamases (ESBLs). In total, 17 ESBL-producers were identified, based on the combination-disk synergy test using cefotaxime and ceftazidime +/- clavulanic acid. Investigation of ESBL genes in 33 ceftazidime-resistant isolates revealed the SHV-12 gene in the same 17 ESBL-producers. In addition, one isolate also carried the CTX-M-3 gene, and two isolates also carried the CTX-M-9 gene. No major epidemic clone of ESBL-producers was identified by pulsed-field gel electrophoresis. Routine screening for the ESBL phenotype, focusing on ceftazidime-resistant E. cloacae, should be undertaken in this area.
authors.affiliation: Department of Critical Care Medicine, Chi-Mei Medical Center, Yungkang, Taiwan.
authors.forename: W-L
authors.initials: WL
authors.lastname: Yu
authors.forename: K-C
authors.initials: KC
authors.lastname: Cheng
authors.forename: C-J
authors.initials: CJ
authors.lastname: Chi
authors.forename: H-E
authors.initials: HE
authors.lastname: Chen
authors.forename: Y-C
authors.initials: YC
authors.lastname: Chuang
authors.forename: L-T
authors.initials: LT
authors.lastname: Wu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology; D019818:Clavulanic Acid / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D016972:Enterobacter cloacae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D013624:Taiwan / Q000453:epidemiology; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D019818:Clavulanic Acid; D002442:Ceftazidime; C412613:CTX-M-9 protein, E coli; C417271:beta-lactamase CTX-M-3; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases