Surface characterisation of various bone cements prepared with functionalised methacrylates/bioactive ceramics in relation to HOB behaviour.
This study reports the relationship between the biocompatibility and surface properties of experimental bone cements. The effect of hydroxyapatite (HA) or alpha-tri-calcium phosphate (alpha-TCP) incorporated into bone cements prepared with methyl methacrylate as base monomer and either methacrylic acid or diethyl amino ethyl methacrylate (DEAEMA) as comonomers was investigated. The in vitro biocompatibility of these composite cements was assessed in terms of the interaction of primary human osteoblasts grown on the materials over a period of 5 days and compared with a control surface. These results were related to the surface properties investigated through a number of techniques, namely Fourier transform infrared, contact angle measurements, X-ray photoelectron spectroscopy and energy dispersive analysis of X-rays. Complementary techniques of thermal analysis and ion chromatography were also performed. Biocompatibility results showed that the addition of alpha-TCP improves biocompatibility regardless of comonomer type. This is in contrast to HA-based cements where cell proliferation was significantly lower. Surface characterisations showed that structural integrity of the materials was maintained in the presence of the acid and base comonomers, and water contact angles were reduced particularly in DEAEMA containing materials. Furthermore, ion chromatography confirmed higher Ca2+ and PO4(3-) ion release by both types of ceramics, particularly for those containing DEAEMA. In conclusion, the incorporation of acidic and basic comonomers to either HA or alpha-TCP ceramics containing bone cements can have differential effects upon the attachment and proliferation of bone cells in vitro. Moreover, those cements consisting of alpha-TCP and containing DEAEMA comonomer indicated the most favourable biocompatibility.
Salih
Vehid
V
UCL Eastman Dental Institute, Division of Biomaterials and Tissue Engineering, University of London, 256 Gray's Inn Road, London WC1X 8LD, UK. v.salih@eastman.ucl.ac.uk
Mordan
Nicky
N
Abou Neel
Ensanya A
EA
Armitage
David A
DA
Jones
Frances H
FH
Knowles
Jonathan C
JC
Nazhat
Showan N
SN
Vargas-Coronado
R
R
Cauich-Rodriguez
Juan V
JV
D001672:Biocompatible Materials; D001843:Bone Cements / Q000737:chemistry*; D002130:Calcium Phosphates; D002455:Cell Division; D002516:Ceramics; D017886:Durapatite; D006801:Humans; D008689:Methacrylates; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014965:X-Rays
D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; D008689:Methacrylates; C485828:alpha-tricalcium phosphate; D017886:Durapatite