Development of beta-tricalcium phosphate/collagen sponge composite for bone regeneration.
Synthetic biomaterials have been developed and used for bone grafting. Here, we developed a biodegradable sponge composite for bone tissue engineering by combining beta-tricalcium phosphate (beta-TCP) and collagen. In addition, we sought to determine the optimal beta-TCP granules/collagen ratio by evaluating and bone formation in vivo. Porous beta-TCP granules were mixed with atelocollagen hydrochloride solution at various ratios--0.02, 0.05, 0.1, and 0.2 g/mL. The resultant mixtures were freeze-dried and subjected to dehydrothermal treatment in vacuo. The final composites obtained were designated beta-TCP/collagen sponge composites (beta-TCP/CS). Through compression testing, it was found that the stress values for beta-TCP/CS (0.2 g/mL) were higher than those of the other three composites over the whole strain range. Histological evaluation at four weeks after implantation revealed that the collagen sponge had degraded and newly formed bone was present on the surface of the beta-TCP granules. At 12 weeks, the beta-TCP granules were completely degraded and remodeling of the lamellar bone was observed.
Matsuno
Tomonori
T
Department of Oral & Maxillofacial Surgery, The Nippon Dental University School of Dentistry at Tokyo, Japan. matsunot@tky.ndu.ac.jp
Nakamura
Tatsuo
T
Kuremoto
Koh-ichi
K
Notazawa
Syunsuke
S
Nakahara
Taka
T
Hashimoto
Yoshiya
Y
Satoh
Tazuko
T
Shimizu
Yasuhiko
Y
D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry* / Q000627:therapeutic use; D002130:Calcium Phosphates / Q000737:chemistry* / Q000627:therapeutic use; D003094:Collagen / Q000737:chemistry* / Q000627:therapeutic use; D019245:Compressive Strength; D004285:Dogs; D008422:Materials Testing; D013552:Swine
D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D003094:Collagen