title: Localization of the mouse defense lectin ficolin B in lysosomes of activated macrophages.
abstract: Ficolins are pattern-recognition molecules of the innate immune system able to trigger the lectin pathway of the complement activation upon binding to microbial surfaces. In humans, two plasma ficolins have been identified and characterized, whereas a third cell-associated ficolin (M-ficolin) was found on monocyte surfaces. The mouse homologue of M-ficolin is called ficolin B. Although the spatial-temporal expression patterns of mouse ficolins have been described recently, the subcellular localization of ficolin B protein is so far unknown. By using ficolin B-specific antibodies and confocal microscopy, we show that ficolin B is expressed within mouse peritoneal exudate macrophages and is co-localized with Lamp-1, a marker for lysosomes and late endosomes. In addition, the data indicate that ficolin B expression is up-regulated upon macrophage activation.
authors.affiliation: Institute of Immunology, University of Regensburg, Regensburg, Germany.
authors.forename: Valeria L
authors.initials: VL
authors.lastname: Runza
authors.forename: Thomas
authors.initials: T
authors.lastname: Hehlgans
authors.forename: Bernd
authors.initials: B
authors.lastname: Echtenacher
authors.forename: Ulrich
authors.initials: U
authors.lastname: Zähringer
authors.forename: Wilhelm J
authors.initials: WJ
authors.lastname: Schwaeble
authors.forename: Daniela N
authors.initials: DN
authors.lastname: Männel
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001853:Bone Marrow / Q000378:metabolism; D002682:Chinchilla; D005260:Female; D047468:Immunoprecipitation; D037102:Lectins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D051907:Lysosomal Membrane Proteins / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D013154:Spleen / Q000378:metabolism; D000097604:Ficolins; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D037102:Lectins; D051907:Lysosomal Membrane Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000097604:Ficolins; C052255:Lamp1 protein, mouse; D052118:Lysosomal-Associated Membrane Protein 1