title: Two mechanistically distinct forms of endocytosis in adrenal chromaffin cells: Differential effects of SH3 domains and amphiphysin antagonism.
abstract: We previously identified two forms of endocytosis using capacitance measurements in chromaffin cells: rapid endocytosis (RE), dynamin-1 dependent but clathrin-independent and slow endocytosis (SE), dynamin-2 and clathrin-dependent. Various recombinant SH3 domains that interact with the proline-rich domain of dynamin were introduced into single cells via the patch pipette. GST-SH3 domains of amphiphysin-1, intersectin-IC, and endophilin-I inhibited SE but had no effect on RE. Grb2-SH3 (N-terminal) or a mutant of amphiphysin-1-SH3 was inactive on either process. These data confirm that dynamin-1 dependent RE is independent of clathrin and show that amphiphysin is exclusively associated with clathrin and dynamin-2-dependent SE.
authors.affiliation: Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA. aelhamda@med.wayne.edu
authors.forename: Abdeladim
authors.initials: A
authors.lastname: Elhamdani
authors.forename: Fouad
authors.initials: F
authors.lastname: Azizi
authors.forename: Elena
authors.initials: E
authors.lastname: Solomaha
authors.forename: H Clive
authors.initials: HC
authors.lastname: Palfrey
authors.forename: Cristina R
authors.initials: CR
authors.lastname: Artalejo
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism; D000311:Adrenal Glands / Q000166:cytology* / Q000502:physiology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002417:Cattle; D019439:Chromaffin Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D002966:Clathrin / Q000378:metabolism; D034284:Dynamin I / Q000378:metabolism; D034285:Dynamin II / Q000378:metabolism; D025802:Electric Capacitance; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D020349:Surface Plasmon Resonance; D018909:src Homology Domains / Q000235:genetics / Q000502:physiology*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D000906:Antibodies; D002966:Clathrin; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C115919:intersectin 1; C075637:amphiphysin; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase; D034284:Dynamin I; D034285:Dynamin II