Non conservation of the meiotic function of the Ski8/Rec103 homolog in Arabidopsis.
Meiotic recombination involves the formation and repair of DNA double-strand breaks (DSB). One of the genes required for DSB formation in the yeast Saccharomyces cerevisiae, Ski8/Rec103, is intriguing because it also plays a role in cytoplasmic RNA metabolism, a function difficult to relate to DSB formation. The meiotic role of Ski8 is conserved in several fungi, but has not been investigated outside this kingdom. We identified the Ski8 homolog in Arabidopsis thaliana and isolated two mutants. We showed that the Arabidopsis Ski8 homolog was required for normal plant development and growth, suggesting a conserved somatic function, but that it was not required for meiotic recombination or progression. The data presented here provide strong evidence that the meiotic role of Ski8 is not conserved in Arabidopsis and sequence analysis suggests that this may also be the case in a range of other species.
Jolivet
Sylvie
S
Station de Génétique et d'Amélioration des Plantes, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles cedex, France.
Vezon
Daniel
D
Froger
Nicole
N
Mercier
Raphaël
R
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D003434:Crossing Over, Genetic; D004706:Endodeoxyribonucleases; D004950:Esterases / Q000235:genetics / Q000378:metabolism; D008540:Meiosis; D008969:Molecular Sequence Data; D009154:Mutation; D017386:Sequence Homology, Amino Acid; D000099257:Meiotic Recombination Protein SPO11
D029681:Arabidopsis Proteins; D004706:Endodeoxyribonucleases; D004950:Esterases; D000099257:Meiotic Recombination Protein SPO11; C513197:SKI8 protein, Arabidopsis