title: [Preparation of 10-hydroxycamptothecin semisolid lipid nanoparticles and investigation of its stability].
abstract: OBJECTIVE
  To prepare 10-hydroxycamptothecin-semisolid lipid nanoparticles (HCPT-SSLN) and investigate its stability.
  
  
  METHODS
  HCPT-SSLN was prepared by the method of "emulsion evaporation at a high temperature and solidification at a low temperature"; The morphology was examined by transmission electron microscope; The particle size and xi potential were determined by laser granularity equipment; The physical stability of both suspl and freeze drying powder of HCPT-SSLN were investigated.
  
  
  RESULTS
  The mean particle size of the prepared HCPT-SSLN was 130.5 nm, drug loading was 2.51%, entrapment efficiency was 79.19%, xi potential was -33.1 mV; Placed at room temperature and 4 degrees C for 6 months, the appearance, particle size and entrapment efficiency of HCPT-SSLN were all stable. Moreover, the freeze drying powder was more stable than the suspl.
  
  
  CONCLUSIONS
  The HCPT-SSLN has high entrapment efficiency and drug loading, uniform particle size, good stability, which initially indicates that HCPT is fit for being incorporated into SSLN.
authors.affiliation: Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Na
authors.initials: N
authors.lastname: Xi
authors.forename: Lian-Bing
authors.initials: LB
authors.lastname: Hou
authors.forename: Xi-Qing
authors.initials: XQ
authors.lastname: Yan
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Jiang
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Wang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D004337:Drug Carriers; D004355:Drug Stability; D005612:Freeze Drying; D008055:Lipids; D049329:Nanostructures; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D011208:Powders; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; D008055:Lipids; D011108:Polymers; D011208:Powders; C028098:10-hydroxycamptothecin; D002166:Camptothecin