The suitable restriction enzymes for pulsed-field gel electrophoresis analysis of Bordetella pertussis.
We searched the restriction enzymes with rare cutting sites on the genome of Bordetella pertussis strain Tohama I using the Restriction Digest Tool software provided in the Institute for Genomic Research web site. The usefulness of 5 enzymes for pulsed-field gel electrophoresis (PFGE) analysis was evaluated with 68 B. pertussis isolates. The results indicated that AflII, DraI, SpeI, and XbaI were useful enzymes, and AflII was the best one for PFGE analysis of B. pertussis isolates.
Lee
Meng-Shiunn
MS
The Third Branch Office, Center for Disease Control, Taichung City 408, Taiwan. mengshiunn@cdc.gov.tw
Lee
Yeong-Sheng
YS
Chiou
Chien-Shun
CS
D001886:Bordetella pertussis / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*
D004262:DNA Restriction Enzymes; C056436:endodeoxyribonuclease SpeI; C025580:endodeoxyribonuclease XBAI; C074326:CTTAAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases