title: In vivo bioluminescence imaging of cord blood derived mesenchymal stem cell transplantation into rat myocardium.
abstract: OBJECTIVE
  The conventional method for the analysis of myocardial cell transplantation depends on postmortem histology. Here, we have sought to demonstrate the feasibility of a longitudinal monitoring of transplanted cell survival in living animals, accomplished with optical imaging techniques and pharmacological interventions.
  
  
  METHODS
  Human cord blood (50 ml) was donated with parental consent. After getting cord blood derived mesenchymal stem cells (CBMSCs), cells were transfected (MOI = 100) overnight with adenovirus encoding firefly luciferase gene (Ad-CMV-Fluc). Our experimental Sprague-Dawley rats (n = 12) were given intramyocardial injections containing 1 x 10(6) CBMSCs, which had been made to express the firefly luciferase (Fluc) reporter gene. Optical bioluminescence imaging was then conducted using a cooled charged-coupled device (CCD) camera (Xenogen), beginning on the day after the transplantation (day 1). Groups of mice were intraperitoneally injected with cyclosporine (5 mg/kg) or tacrolimus (1 mg/kg), in an attempt to determine the degree to which cell survival had been prolonged, and these values were then compared with the cell survival values of the negative control group. The presence of transplanted CBMSCs on in vivo images confirmed by in situ hybridization for human specific Alu in the myocardium.
  
  
  RESULTS
  Cardiac bioluminescence signals were determined to be present for 6 days after transplantation: day 1 (97000 +/- 9100 x 10(5) p/s/cm2/sr), day 3 (9600 +/- 1110 p/s/cm2/sr), and day 5 (3200 +/- 550 p/s/cm2/sr). The six mice that received either cyclosporine or tacrolimus displayed cardiac bioluminescence signals for a period of 8 days after transplantation. We observed significant differences between the treated group and the non-treated group, beginning on day 3 (tacrolimus; 26500 +/- 4340 p/s/cm2/sr, cyclosporine; 27200 +/- 3340 p/s/cm2/sr, non-treated; 9630 +/- 1180 p/s/cm2/sr, p < 0.01), and persisting until day 7 (tacrolimus; 12500 +/- 2946 p/s/cm2/sr, cyclosporine; 7310 +/- 1258 p/s/cm2/sr, non-treated; 2460 +/- 160 p/s/cm2/sr, p < 0.01). The human-derived CBMSCs were detected in the myocardium 3 days after transplantation by in situ hybridization.
  
  
  CONCLUSIONS
  The locations, magnitude, and survival duration of the CBMSCs were noninvasively monitored with a bioluminescence optical imaging system. We determined that optical molecular imaging expedites the fast throughput screening of pharmaceutical agents, allowing for the noninvasive tracking of cell survival within animals. In rat cardiac CBMSC transplant models, transient immunosuppressive treatment with tacrolimus or cyclosporine was shown to improve donor cell survival.
authors.affiliation: Institute for Molecular Photonic Imaging Research, Chonnam National University Hospital, Republic of Korea.
authors.forename: Jung-Joon
authors.initials: JJ
authors.lastname: Min
authors.forename: Youngkeun
authors.initials: Y
authors.lastname: Ahn
authors.forename: Sungmin
authors.initials: S
authors.lastname: Moon
authors.forename: Yong Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Jong Eun
authors.initials: JE
authors.lastname: Park
authors.forename: Sung Mi
authors.initials: SM
authors.lastname: Kim
authors.forename: Uyenchi N
authors.initials: UN
authors.lastname: Le
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Wu
authors.forename: Soo Yeon
authors.initials: SY
authors.lastname: Joo
authors.forename: Moon Hwa
authors.initials: MH
authors.lastname: Hong
authors.forename: Deok Hwan
authors.initials: DH
authors.lastname: Yang
authors.forename: Myung Ho
authors.initials: MH
authors.lastname: Jeong
authors.forename: Chang Hun
authors.initials: CH
authors.lastname: Song
authors.forename: Yun Hyeok
authors.initials: YH
authors.lastname: Jeong
authors.forename: Kyung Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Kang
authors.forename: Hee-Seung
authors.initials: HS
authors.lastname: Bom
mesh_terms: D000818:Animals; D006348:Cardiac Surgical Procedures / Q000379:methods; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D005240:Feasibility Studies; D008163:Luminescent Measurements / Q000379:methods*; D008164:Luminescent Proteins / Q000032:analysis; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008164:Luminescent Proteins