T-h-2 immunity and CD3+CD45RBlow-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope.
The genetic engineering of Mycobacterium bovis-bacillus Calmette-Guérin to express foreign epitopes is an attractive strategy in the field of epitope vaccines. We constructed an 'epitope-trap vector' with Mycobacterium tuberculosis chaperonin-10 as a carrier antigen and used it to express the HIV-1 principal neutralizing determinant epitope. We also identified a new chaperonin-10 promoter that was hyperexpressive compared with the heat shock protein-65 promoter. Splenocytes from recombinant bacillus Calmette-Guérin-immunized mice showed enhanced lymphocyte proliferation and interleukin-4 (but not interferon-gamma) secretion. The recombinant bacillus Calmette-Guérin-immunized group also exhibited mild delayed-type hypersensitivity reaction and a high frequency of CD3+CD45RBlow-activated T cells, together with high titer of antiprincipal neutralizing determinant immunoglobulin G antibodies. Thus, this epitope delivery system induced strong epitope-specific T-h-2 polarization.
Aravindhan
Vivekanandhan
V
Department of Immunology, Tuberculosis Research Centre, Chennai, India.
Narayanan
Sujatha
S
Gautham
Namasivayam
N
Prasad
Vinayaka
V
Kannan
P
P
Jacobs
William R
WR
Narayanan
Paranjir
P
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001500:BCG Vaccine / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D017252:CD3 Complex / Q000276:immunology*; D018835:Chaperonin 10 / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007114:Immunization; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
D001500:BCG Vaccine; D017252:CD3 Complex; D018835:Chaperonin 10; D016207:Cytokines; D016056:Immunodominant Epitopes; D017493:Leukocyte Common Antigens