title: Vitellogenin gene expression as a biomarker of endocrine disruption in the invertebrate, Mytilus edulis.
abstract: Vitellogenin levels are often used as a biomarker of endocrine disruption in fish. For invertebrates, there is a general lack of knowledge regarding endocrine regulation and, consequently, there are few direct biomarkers of endocrine disruption in such species. This study focuses on the marine mussel Mytilus edulis, which is often employed in biomonitoring studies. A partial vitellogenin mRNA has been isolated and gene expression quantified using real-time PCR.
authors.affiliation: Department of Biology and Environmental Science, School of Life Sciences, University of Sussex, Falmer, Brighton, BN1 9QJ, UK.
authors.forename: Alin-Mirel
authors.initials: AM
authors.lastname: Puinean
authors.forename: Jeanette M
authors.initials: JM
authors.lastname: Rotchell
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D017931:DNA Primers / Q000737:chemistry; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006066:Gonads / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D049880:Mytilus edulis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012737:Sex Factors; D014819:Vitellogenins / Q000096:biosynthesis / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D017931:DNA Primers; D052244:Endocrine Disruptors; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004958:Estradiol