Synthesis of ordered large pore SBA-15 spherical particles for adsorption of biomolecules.
The synthesis of spherical particles of mesoporous silicates (SBA-15) with mesopore diameter upto 127A, and particle diameter of 4-10 microm has been achieved. The SBA-15 spheres were obtained using pluronics P123 (EO20PO70EO20) as a surfactant coupled with cetyltrimethylammonium bromide (CTAB) as a co-surfactant. Ethanol played a very important role in the formation of silica spheres, as it delays the reaction rate of the SBA-15 synthesis. A wide range of pore diameters (28-127 A) of these spherical SBA-15 materials with large surface area >700 m2/g has been synthesized. The effects of temperature, ethanol, CTAB and swelling agent have also been studied. The SBA-15 samples were characterized using small angle X-ray scattering (SAXS), N2 adsorption-desorption, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The spherical SBA-15 after mechanical grinding was used for protein adsorption, and adsorption capacity was compared with that of conventional fibrous SBA-15. The spherical SBA-15 particles with pore diameter of 127 A have a very high capacity of 700 mg/g for lysozyme at pH 7.
Katiyar
Amit
A
Department of Chemical & Materials Engineering, University of Cincinnati, 401 Rhodes Hall, Cincinnati, OH 45221-0012, USA.
Yadav
Santosh
S
Smirniotis
Panagiotis G
PG
Pinto
Neville G
NG
D000327:Adsorption; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008863:Microspheres; D009113:Muramidase / Q000737:chemistry; D010316:Particle Size; D016062:Porosity; D015203:Reproducibility of Results; D017640:Silicates / Q000138:chemical synthesis* / Q000737:chemistry; D012822:Silicon Dioxide / Q000138:chemical synthesis* / Q000737:chemistry; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry; D014961:X-Ray Diffraction / Q000379:methods
C509969:SBA-15; D017640:Silicates; D013501:Surface-Active Agents; D012822:Silicon Dioxide; D009113:Muramidase