title: Allergic eosinophilic gastroenteritis with protein-losing enteropathy: intestinal pathology, clinical course, and long-term follow-up.
abstract: OBJECTIVE
  A subset of patients with allergic eosinophilic gastroenteritis (AEG) has anemia and hypoalbuminemia caused by protein-losing enteropathy (PLE). Our goals were to describe the response to therapy and the long-term outcome of patients in this subgroup and to evaluate their gastric and intestinal biopsies for distinguishing features that might explain their protein and blood loss.
  
  
  METHODS
  Patients with AEG + PLE were identified retrospectively and compared with controls and with patients with AEG only. Immunohistochemical staining for tryptase, a mast cell mediator, was performed on gastric and duodenal tissues. Eosinophils identified by hematoxylin/eosin stain and mast cells identified as tryptase-positive cells were counted in one high-power field area with maximal cell infiltration.
  
  
  RESULTS
  Although all patients had excellent response to therapy with amino acid-based formula and tolerated gradual introduction of some foods with time, food-responsive disease persisted in all patients over 2.5 to 5.5 years of follow-up. Routine histological evaluation did not show any features differentiating AEG + PLE from AEG. When eosinophils and mast cells were counted in intestinal biopsies, however, significantly more mast cells were found in biopsies of the AEG + PLE group despite comparable numbers of eosinophils. In contrast, in gastric biopsies, eosinophils were more prominent in AEG + PLE, but mast cell numbers were similar in all groups.
  
  
  CONCLUSIONS
  Patients with AEG + PLE responded well to therapy with amino acid-based formula. Food hypersensitivities did not completely resolve over up to 5.5 years. Intestinal mast cells were significantly increased in maximally infiltrated areas of the intestine, possibly causing increased intestinal permeability and protein loss.
authors.affiliation: Pediatric Allergy and Immunology, Mount Sinai School of Medicine, New York, NY 10029, USA. mirna.chehade@mssm.edu
authors.forename: Mirna
authors.initials: M
authors.lastname: Chehade
authors.forename: Margret S
authors.initials: MS
authors.lastname: Magid
authors.forename: Shideh
authors.initials: S
authors.lastname: Mofidi
authors.forename: Anna
authors.initials: A
authors.lastname: Nowak-Wegrzyn
authors.forename: Hugh A
authors.initials: HA
authors.lastname: Sampson
authors.forename: Scott H
authors.initials: SH
authors.lastname: Sicherer
mesh_terms: D004804:Eosinophils; D005759:Gastroenteritis / Q000150:complications / Q000473:pathology*; D006801:Humans; D007422:Intestines / Q000473:pathology*; D011504:Protein-Losing Enteropathies / Q000150:complications / Q000473:pathology*; D012189:Retrospective Studies; D013270:Stomach / Q000473:pathology*