Identification and functional validation of novel autoantigens in equine uveitis.
The development, progression, and recurrence of autoimmune diseases are frequently driven by a group of participatory autoantigens. We identified and characterized novel autoantigens by analyzing the autoantibody binding pattern from horses affected by spontaneous equine recurrent uveitis to the retinal proteome. Cellular retinaldehyde-binding protein (cRALBP) had not been described previously as autoantigen, but subsequent characterization in equine recurrent uveitis horses revealed B and T cell autoreactivity to this protein and established a link to epitope spreading. We further immunized healthy rats and horses with cRALBP and observed uveitis in both species with typical tissue lesions at cRALBP expression sites. The autoantibody profiling outlined here could be used in various autoimmune diseases to detect autoantigens involved in the dynamic spreading cascade or serve as predictive markers.
Deeg
Cornelia A
CA
Institute of Animal Physiology, Ludwig Maximilians University (LMU) Munich, Veterinärstr. 13, D-80539 Munich, Germany.
Pompetzki
Dirk
D
Raith
Albert J
AJ
Hauck
Stefanie M
SM
Amann
Barbara
B
Suppmann
Sabine
S
Goebel
Thomas W F
TW
Olazabal
Ursula
U
Gerhards
Hartmut
H
Reese
Sven
S
Stangassinger
Manfred
M
Kaspers
Bernd
B
Ueffing
Marius
M
D000818:Animals; D001324:Autoantigens / Q000276:immunology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000276:immunology / Q000378:metabolism*; D006734:Horse Diseases / Q000276:immunology* / Q000473:pathology; D006736:Horses; D007074:Immunoglobulin G / Q000378:metabolism; D011485:Protein Binding; D020543:Proteome / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D012160:Retina / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013601:T-Lymphocytes / Q000276:immunology; D014605:Uveitis / Q000276:immunology* / Q000473:pathology / Q000662:veterinary*
C020932:11-cis-retinal-binding protein; D001324:Autoantigens; D002352:Carrier Proteins; D005136:Eye Proteins; D007074:Immunoglobulin G; D020543:Proteome