title: The relationship between uric acid and its oxidative product allantoin: a potential indicator for the evaluation of oxidative stress in birds.
abstract: Uric acid is the main nitrogenous waste product in birds but it is also known to be a potent antioxidant. Hominoid primates and birds lack the enzyme urate oxidase, which oxidizes uric acid to allantoin. Consequently, the presence of allantoin in their plasma results from non-enzymatic oxidation. In humans, the allantoin to uric acid ratio in plasma increases during oxidative stress, thus this ratio has been suggested to be an in vivo marker for oxidative stress in humans. We measured the concentrations of uric acid and allantoin in the plasma and ureteral urine of white-crowned sparrows (Zonotrichia leucophrys gambelii) at rest, immediately after 30 min of exercise in a hop/hover wheel, and after 1 h of recovery. The plasma allantoin concentration and the allantoin to uric acid ratio did not increase during exercise but we found a positive relationship between the concentrations of uric acid and allantoin in the plasma and in the ureteral urine in the three activity phases. In the plasma, the slope of the regression describing the above positive relationships was significantly higher immediately after activity. We suggest that the slope indicates the rate of uric acid oxidation and that during activity this rate increases as a result of higher production of free radicals. The present study demonstrates that allantoin is present in the plasma and in the ureteral urine of white-crowned sparrows and therefore might be useful as an indicator of oxidative stress in birds.
authors.affiliation: Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel. elat@techunix.technion.ac.il
authors.forename: Ella
authors.initials: E
authors.lastname: Tsahar
authors.forename: Zeev
authors.initials: Z
authors.lastname: Arad
authors.forename: Ido
authors.initials: I
authors.lastname: Izhaki
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Guglielmo
mesh_terms: D000481:Allantoin / Q000097:blood / Q000378:metabolism* / Q000652:urine; D000818:Animals; D009043:Motor Activity; D018384:Oxidative Stress / Q000502:physiology*; D010101:Oxygen Consumption; D010805:Physical Conditioning, Animal; D046370:Sparrows / Q000502:physiology*; D014513:Ureter; D014527:Uric Acid / Q000097:blood / Q000378:metabolism* / Q000652:urine
chemical_list: D014527:Uric Acid; D000481:Allantoin