Long-term fate and distribution of newborn cells in the adult mouse olfactory bulb: Influences of olfactory deprivation.
The adult subventricular zone produces neuroblasts that migrate to the main olfactory bulb, where they differentiate into interneurons in the glomerular and granular layers. Using bromodeoxyuridine labeling, the survival of newborn cells was assessed in these two layers of the MOB in control mice and in mice unilaterally deprived from sensory input by naris occlusion. In control main olfactory bulbs, bromodeoxyuridine-positive cell density decreased about 70% between 15 and 180 days post-bromodeoxyuridine administration but earlier in the glomerular layer than in the granular layer. At all time points examined, newborn cell density was higher in the deep granular layer than in the superficial granular layer. Occlusion started at the age of 2 months and lasted for 15, 30, 45, 60 or 180 days. The newborn cell survival was similarly reduced in both layers by occlusion, during a critical period 15 and 45 days post-occlusion. Interestingly, olfactory deprivation decreased bromodeoxyuridine-positive cell density in the deep granular layer only, indicating a greater dependence of cell fate on sensory input in this sub-layer. Neuronal differentiation was assessed in the granular layer and glomerular layer by multiple double-labeling 45 days post-bromodeoxyuridine-injections, the time point at which the proportion of bromodeoxyuridine-positive cells expressing a neuronal marker reached approximately 85% in the granular layer and approximately 50% in the glomerular layer. Naris occlusion did not significantly affect these proportions. Taken together, our results reveal that the survival of newborn cells has a different time course in the glomerular layer and in the granular layer, but is similarly decreased in each layer by olfactory deprivation. In addition, our data suggest a functional heterogeneity of neurogenesis within the granular layer.
Mandairon
N
N
Laboratoire de Neurosciences et Systèmes Sensoriels, CNRS UMR 5020, Université Claude Bernard Lyon 1, 50 Avenue Tony Garnier, 6936, Lyon Cedex 07, France. nm243@cornell.edu
Sacquet
J
J
Jourdan
F
F
Didier
A
A
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine / Q000378:metabolism; D064026:Calbindins; D002452:Cell Count / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology* / Q000502:physiology*; D009833:Olfactory Pathways / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D012683:Sensory Deprivation / Q000502:physiology*; D012903:Smell / Q000502:physiology*; D013997:Time Factors
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine