Analysis of leptin signalling in hematopoietic cells using an adapted MAPPIT strategy.
The adipocyte-secreted hormone leptin participates in the regulation of hematopoiesis and enhances proliferation of hematopoietic cells. We used an adaptation of the MAPPIT mammalian two-hybrid method to study leptin signalling in a hematopoietic setting. We confirmed the known interactions of suppressor of cytokine signalling 3 (SOCS3) and STAT5 with the Y985 and Y1077 motifs of the leptin receptor, respectively. We also provide evidence for novel interactions at the Y1077 motif, including phospholipase C gamma and several members of the SOCS protein family, further underscoring the important role of the Y1077 motif in leptin signalling.
Montoye
T
T
Flanders Interuniversity Institute for Biotechnology, Department of Medical Protein Research, Faculty of Medicine and Health Sciences, Ghent University, A. Baertsoenkaai 3, B-9000 Ghent, Belgium.
Piessevaux
J
J
Lavens
D
D
Wauman
J
J
Catteeuw
D
D
Vandekerckhove
J
J
Lemmens
I
I
Tavernier
J
J
D020816:Amino Acid Motifs; D000818:Animals; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D020738:Leptin / Q000378:metabolism; D051379:Mice; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D054411:Receptors, Leptin; D050799:STAT5 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
C515448:LEPR protein, human; D020738:Leptin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D050799:STAT5 Transcription Factor; D050826:Suppressor of Cytokine Signaling Proteins; C515447:leptin receptor, mouse