Immunomodulatory properties of Cumaside.
The medical lead, so-called Cumaside, was created on the basis of triterpene oligoglycosides from the Far-Eastern edible sea cucumber (holothurian) Cucumaria japonica and its immunomodulatory properties were studied. The haemolytic activity of Cumaside was significantly reduced in comparison with original glycosides due to the glycoside-cholesterol complex formation. The influence of Cumaside on mouse macrophages in low doses was accompanied by more then two-fold stimulation of lysosomal activity. This preparation was found to increase significantly the animal resistance against bacterial infections elicited by various pathogens. It stimulated phagocytosis, ROS formation, IL6 and TNF-alpha production in lymphocytes, increased the number of antibody producing cells and amplified the expression of several cell surface molecules (CD3, CD4, CD8) preliminary cultured with hydrocortisone. At the same time the preparation did not affect the delayed-type hypersensitivity, proliferative activity of lymphocytes, cytotoxic activity of NK-cells and cytokine IFNgamma and IL12p70 release. The mechanism of Cumaside action is discussed.
Aminin
D L
DL
Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of RAS, 690022, Vladivostok, Russia. daminin@piboc.dvo.ru
Pinegin
B V
BV
Pichugina
L V
LV
Zaporozhets
T S
TS
Agafonova
I G
IG
Boguslavski
V M
VM
Silchenko
A S
AS
Avilov
S A
SA
Stonik
V A
VA
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D048016:Cucumaria / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects; D006027:Glycosides; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology; D008247:Lysosomes / Q000187:drug effects / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000472:pathogenicity; D014315:Triterpenes / Q000494:pharmacology*; D015011:Yersinia pseudotuberculosis / Q000472:pathogenicity; D015012:Yersinia pseudotuberculosis Infections / Q000517:prevention & control*
D015703:Antigens, CD; D006027:Glycosides; D014315:Triterpenes; C033504:cucumarioside