Application of cell-based biosensors for the detection of bacterial elicitor flagellin.
Cell-based biosensors, bioelectronic portable devices containing plant living cells have been used for monitoring some physiological changes induced by pathogen-derived signal molecules called flagellin. The screen-printed electrodes have been adapted for preparation of biosensors. The proton-sensitive thick films have been printed using composite bulk modified with edition of RuO(2). Obtained disposable electrodes were made possible to measure the pH change with well sensitivity and reproducibility. Tobacco cells attached to the electrode surface, cell-based biosensor, can be used for the detection of flagellin, the virulence factor of bacterial pathogen. We culture tobacco cells on the surface of such electrotransducer for several weeks and monitor of potential of cells under flagellin stimulation. The detection of the electrochemical proton gradient across the plasma membrane serves as the analytical signal. The electrode response depended upon H(+) concentration in extracellular solution. It can be conveniently observed on the surfaces of biosensors. Suitable stability and the good response time of constructed biosensors were observed. Future development of these cell-based biosensors could draw advances in selective monitoring of microbial pathogens and other physiologically active components. Moreover, this new method is much faster compared with the traditional microbial testing.
Oczkowski
T
T
Laboratory of Proteomics, Institute of Plant Genetics, Polish Academy of Science, Poznań, Poland.
Zwierkowska
E
E
Bartkowiak
S
S
D016960:Agrobacterium tumefaciens / Q000737:chemistry / Q000378:metabolism*; D000468:Alkalies; D015374:Biosensing Techniques / Q000379:methods*; D004566:Electrodes; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D005408:Flagellin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D014026:Nicotiana
D000468:Alkalies; D005408:Flagellin