Identification of 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue from the sheath of Leptothrix cholodnii.
The sheath of Leptothrix cholodnii is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was S-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue.
Makita
Hiroko
H
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University, Japan. mtake@ynu.ac.jp
Nakahara
Yuichi
Y
Fukui
Hiroyuki
H
Miyanoiri
Yohei
Y
Katahira
Masato
M
Seki
Hiroko
H
Takeda
Minoru
M
Koizumi
Jun-Ichi
J
D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D006603:Hexuronic Acids / Q000032:analysis*; D042743:Leptothrix / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010069:Oxadiazoles / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*
C512926:2-(cysteinyl)amido-2-deoxy-D-galacturonic acid; D006603:Hexuronic Acids; D010069:Oxadiazoles; D011135:Polysaccharides, Bacterial; C046364:4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole; D003545:Cysteine