An improved method for suppressing protein background in PFG NMR experiments to determine ligand diffusion coefficients in the presence of receptor.
In NMR diffusion experiments to study ligand-protein binding equilibria, the spectral background due to broad protein resonances can contribute significantly to the measured ligand signal intensity resulting in erroneous binding affinities. One method to suppress the protein spectral background involves coupling a CPMG pulse train before or after the BPPSTE pulse sequence to allow for differential T(2) relaxation of the broad protein resonances. Here, we present an improved method, the Gradient Phase Encoded Spin-lock (GraPES) experiment that integrates the relaxation filter into the diffusion period. Compared with sequential CPMG-BPPSTE pulse sequences, GraPES offers effective suppression of the protein background with improved signal-to-noise ratios and shorter experiment times.
Becker
Bridget A
BA
Department of Chemistry, University of California-Riverside, 328 Physical Sciences Building 1, Riverside, CA 92521-0403, USA.
Morris
Kevin F
KF
Larive
Cynthia K
CK
D004058:Diffusion; D006023:Glycoproteins / Q000737:chemistry*; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011433:Propranolol / Q000737:chemistry*; D011485:Protein Binding
D006023:Glycoproteins; D008024:Ligands; D011433:Propranolol