title: Quantitative GC-ECD analysis of halogenated metabolites: determination of surface and within-thallus elatol of Laurencia obtusa.
abstract: Information on natural concentrations or variability of secondary metabolites in marine organisms may be important both to ecological/evolutionary and applied approaches. A gas chromatographic procedure with an electron capture detector (GC-ECD) was developed to quantify the sesquiterpenoid elatol at the surface and within-thalli of 70 specimens of the red seaweed Laurencia obtusa. The concentration of elatol was highest within-thalli [9.89 mg g(-1) of L. obtusa, dry weight (d.w.)], compared to lower values found at the surface [0.006 mg g(-1) of L.obtusa (d.w.), or 0.5-10.0 ng cm(-2)]. This method provides a rapid and inexpensive quantification of small quantities of elatol, and probably may also be used to quantify other halogenated compounds usually found in red seaweeds.
authors.affiliation: Departamento de Biologia Marinha, Universidade Federal Fluminense, Caixa Postal 100644, CEP 24001-970 Niterói, Rio de Janeiro, Brazil.
authors.forename: Daniela Bueno
authors.initials: DB
authors.lastname: Sudatti
authors.forename: Silvana Vianna
authors.initials: SV
authors.lastname: Rodrigues
authors.forename: Renato Crespo
authors.initials: RC
authors.lastname: Pereira
mesh_terms: D002849:Chromatography, Gas; D044722:Laurencia / Q000737:chemistry* / Q000378:metabolism; D013141:Spiro Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D013141:Spiro Compounds; C485576:elatol