Characterization of bacteriocin HV219, produced by Lactococcus lactis subsp. lactis HV219 isolated from human vaginal secretions.
Bacteriocin HV219, produced by Lactococcus lactis subsp. lactis HV219, is active against Gram-positive and Gram-negative bacteria. Activity was lost when treated with proteolytic enzymes, SDS, Triton X-114 and Triton X-100, but not at pH 2.0 to 10.0 or after 20 min at 121 degrees C. Growth in the presence of yeast extract as sole nitrogen source yielded 3200 AU/ml. No bacHV219 activity was recorded in MRS broth with maltose, mannose, lactose or sucrose as sole carbohydrate, but fructose yielded 1600 AU/ml. K(2)HPO(4) at 10.0 g/l yielded 3200 AU/ml. Addition of 1.0 mg/l cyanocobalamin, l-ascorbic acid and thiamine to MRS broth yielded 3200 AU/ml, 1600 AU/ml and 1600 AU/ml, respectively. The mode of activity is bacteriolytic, as confirmed by atomic force microscopy.
Todorov
Svetoslav D
SD
Department of Microbiology, University of Stellenbosch, 7600 Stellenbosch, South Africa.
Danova
Svetla T
ST
Van Reenen
Carol A
CA
Meincken
Martina
M
Dinkova
Galina
G
Ivanova
Iskra V
IV
Dicks
Leon M T
LM
D000328:Adult; D001430:Bacteriocins / Q000096:biosynthesis / Q000494:pharmacology*; D001433:Bacteriolysis; D003470:Culture Media; D005260:Female; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000502:physiology; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D013294:Lactococcus lactis / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D014621:Vagina / Q000378:metabolism / Q000382:microbiology*
D001430:Bacteriocins; D003470:Culture Media