Analysis of variabilities of serum proteomic spectra in patients with gastric cancer before and after operation.
OBJECTIVE
To study the variabilities of serum proteomic spectra in patients with gastric cancer before and after operation in order to detect the specific protein markers that can be used for quick diagnosis of gastric cancer.


METHODS
Proteomic spectra of 46 serum samples from patients with gastric cancer before and after operation and 40 from normal individuals were generated by IMAC-Cu protein chip and surface-enhanced laser desorption/ ionization time of flight mass spectrometry.


RESULTS
Fourteen differentially expressed proteins in serum were screened by analysis of proteomic spectra of preoperative patients and normal individuals. We obtained 4 proteins (heat shock protein 27, glucose-regulated protein, prohibitin, protein disulfide isomerase A3) making up marker pattern which was able to class the patient-team and normal-team. These marker patterns yielded 95.7% sensitivity and 92.5% specificity, respectively. The proteins over-expressed in serum of preoperative patients were obviously down-regulated.


CONCLUSIONS
Specific protein markers of gastric cancer can be used for the quick diagnosis of gastric cancer and judgment of prognosis. SELDI-TOF-MS is a useful tool for the detection and identification of new protein markers in serum.
Ren
Hong
H
Department of Oncosurgery, First Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. lightingboy532@163.com
Du
Ning
N
Liu
Gang
G
Hu
Heng-Tong
HT
Tian
Wei
W
Deng
Zhi-Ping
ZP
Shi
Jing-Sen
JS
D014408:Biomarkers, Tumor / Q000097:blood; D001798:Blood Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000097:blood / Q000235:genetics; D006360:Heat-Shock Proteins / Q000097:blood / Q000235:genetics; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000097:blood / Q000235:genetics; D011182:Postoperative Care; D011300:Preoperative Care; D011379:Prognosis; D000091402:Prohibitins; D040081:Protein Array Analysis; D019704:Protein Disulfide-Isomerases / Q000097:blood / Q000235:genetics; D040901:Proteomics; D012097:Repressor Proteins / Q000097:blood / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000601:surgery*
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D000091402:Prohibitins; D012097:Repressor Proteins; C018551:glucose-regulated proteins; D019704:Protein Disulfide-Isomerases; C085868:PDIA3 protein, human