title: Skin capacitance imaging and corneosurfametry. A comparative assessment of the impact of surfactants on stratum corneum.
abstract: Silicon image sensor (SIS) technology was recently introduced as an innovative tool (SkinChip, L'Oréal) providing sensitive imaging of the skin capacitance. This method can detect discrete focal variations in skin surface hydration, and thus early discrete manifestations of skin irritation induced by surfactants. In the present in vivo study, 2 neat and diluted shampoos, and 5% and 10% sodium laurylsulfate solutions were tested on human skin. Each surfactant solution was gently rubbed on the skin using wet hair wicks mimicking the casual use of a shampoo on the scalp. Clinical and SIS evaluations were carried out. In addition, the same products were tested using the ex vivo corneosurfametry bioassay performed on human stratum corneum (SC) harvested by cyanoacrylate skin surface strippings. The colourimetric index of mildness (CIM) was measured on these samples. The product reactivity with the SC was recognized by darker skin capacitance images, and by both lowered SkinChip-generated values and lowered CIM values. The extent in changes varied according to the nature of the test products and their concentrations. The SkinChip image changes likely corresponded to the acute surfactant-induced water swelling of the corneocytes. Skin capacitance imaging and corneosurfametry allow to disclose discrete surfactant-induced alterations of corneocytes.
authors.affiliation: Departement of Dermatopathology, University hospital of Liège, Belgium.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Xhauflaire-Uhoda
authors.forename: Geneviève
authors.initials: G
authors.lastname: Loussouarn
authors.forename: Christelle
authors.initials: C
authors.lastname: Haubrechts
authors.forename: Didier Saint
authors.initials: DS
authors.lastname: Léger
authors.forename: Gérald E
authors.initials: GE
authors.lastname: Piérard
mesh_terms: D017449:Dermatitis, Allergic Contact / Q000175:diagnosis* / Q000209:etiology / Q000503:physiopathology; D004311:Double-Blind Method; D025802:Electric Capacitance; D006203:Hair Preparations / Q000009:adverse effects / Q000494:pharmacology; D006801:Humans; D012867:Skin / Q000187:drug effects*; D012879:Skin Physiological Phenomena / Q000187:drug effects; D012882:Skin Tests / Q000295:instrumentation* / Q000379:methods; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000009:adverse effects / Q000494:pharmacology*; D014870:Water Loss, Insensible / Q000187:drug effects*
chemical_list: D006203:Hair Preparations; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C028913:dodecyl sulfate