title: 1,25-Dihydroxyvitamin D3 suppresses exportin expression in human promyelocytic leukemia HL-60 cells.
abstract: The active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3 (DVD), is a potent inducer of cell differentiation and inhibits proliferation of cells such as human promyelocytic leukemia HL-60 cells. In the present study, we examined the effects of DVD on the expression of exportin-1 and exportin-t, which play essential roles in the transport of mRNA and tRNA, respectively. The results of reverse transcription-polymerase chain reaction and quantitative real-time polymerase chain reaction indicated that DVD down-regulated the gene expression of these exportins. Western blotting revealed the decreased production of these proteins in DVD-treated cells. Thus, the present findings of decreased expression of exportin-1 and exportin-t induced by DVD can be correlated to inhibition of the proliferation of HL-60 cells.
authors.affiliation: Laboratory of Cellular Biochemistry, Graduate School of Nutritional and Environmental Sciences, and COE for the 21th Century, University of Shizuoka, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yu
authors.initials: Y
authors.lastname: Koyama
authors.forename: Sumio
authors.initials: S
authors.lastname: Hayakawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Munakata
authors.forename: Mamoru
authors.initials: M
authors.lastname: Isemura
mesh_terms: D049109:Cell Proliferation; D015973:Gene Expression Regulation, Leukemic; D018922:HL-60 Cells; D006801:Humans; D028884:Karyopherins / Q000235:genetics / Q000378:metabolism*; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000378:metabolism; D000097606:Exportin 1 Protein
chemical_list: D028884:Karyopherins; D029543:Nucleocytoplasmic Transport Proteins; D012333:RNA, Messenger; D012343:RNA, Transfer; D018160:Receptors, Cytoplasmic and Nuclear; D014807:Vitamin D; D000097606:Exportin 1 Protein; C503618:XPOT protein, human; C118756:dihydroxy-vitamin D3