title: Molecular imaging reveals skeletal engraftment sites of transplanted bone marrow cells.
abstract: Molecular imaging holds great promise for the in vivo study of cell therapy. Our hypothesis was that multimodality molecular imaging can identify the initial skeletal engraftment sites post-bone marrow cell transplantation. Utilizing a standard mouse model of bone marrow (BM) transplantation, we introduced a combined bioluminescence (BLI) and positron emission tomography (PET) imaging reporter gene into mouse bone marrow cells. Bioluminescence imaging was used for monitoring serially the early in vivo BM cell engraftment/expansion every 24 h. Significant cell engraftment/expansion was noted by greatly increased bioluminescence about 1 week posttransplant. Then PET was applied to acquire three-dimensional images of the whole-body in vivo biodistribution of the transplanted cells. To localize cells in the skeleton, PET was followed by computed tomography (CT). Co-registration of PET and CT mapped the sites of BM engraftment. Multiple, discrete BM cell engraftment sites were observed. Taken together, this multimodality approach may be useful for further in vivo characterization of various therapeutic cell types.
authors.affiliation: In Vivo Cellular Molecular Imaging Center, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
authors.forename: Philipp
authors.initials: P
authors.lastname: Mayer-Kuckuk
authors.forename: Mikhail
authors.initials: M
authors.lastname: Doubrovin
authors.forename: Luc
authors.initials: L
authors.lastname: Bidaut
authors.forename: Tulin
authors.initials: T
authors.lastname: Budak-Alpdogan
authors.forename: Shangde
authors.initials: S
authors.lastname: Cai
authors.forename: Vanessa
authors.initials: V
authors.lastname: Hubbard
authors.forename: Onder
authors.initials: O
authors.lastname: Alpdogan
authors.forename: Marcel
authors.initials: M
authors.lastname: van den Brink
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Bertino
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Blasberg
authors.forename: Debabrata
authors.initials: D
authors.lastname: Banerjee
authors.forename: Juri
authors.initials: J
authors.lastname: Gelovani
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D016026:Bone Marrow Transplantation / Q000379:methods* / Q000592:standards; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000378:metabolism*; D017930:Genes, Reporter / Q000502:physiology; D021621:Imaging, Three-Dimensional; D008024:Ligands; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D049268:Positron-Emission Tomography / Q000379:methods*; D012190:Retroviridae / Q000235:genetics; D014057:Tomography, X-Ray Computed / Q000379:methods*; D014161:Transduction, Genetic / Q000379:methods / Q000592:standards
chemical_list: D008024:Ligands