title: Low prevalence of transfusion transmitted virus (TTV)-like DNA sequences in cystadenolymphoma and pleomorphic adenoma of the salivary glands.
abstract: Titers of transfusion transmitted virus (TTV)-like DNA in saliva samples have been reported 100-1,000 times higher than those of the corresponding sera, suggesting viral transmission by saliva droplets. The present study was conducted to determine whether TTV-like DNA sequence elements play a role in the pathogenesis of cystadenolymphoma or pleomorphic adenoma and if the parotid or the submandibular gland is a major source of TTV persistence. Sixty-two archival salivary gland samples (16 cystadenolymphomas, 13 pleomorphic adenomas, and 33 controls) and 23 corresponding saliva samples were examined using a polymerase chain reaction (PCR) for TTV DNA. All PCR products that displayed DNA bands were sequenced. Leder's stain and immunohistochemistry (anti-CD8, anti-CD20, anti-CD45R0, anti-CD68, and anti-Ki67/MiB1) were applied to detect possible changes associated with findings of TTV-like DNA sequences. Tissue displayed TTV-like DNA sequences in 8.1% (5/62; saliva: 47.8%, 11/23). Tissue that contained TTV-like DNA sequences was histologically indistinguishable from samples lacking such DNA. TTV appears to be only a bystander in cystadenolymphoma, pleomorphic adenoma, and other salivary gland affections. Neither of the glands seems to be a major source of TTV persistence.
authors.affiliation: Department of General and Special Pathology, Saarland University, 66421, Homburg-Saar, Germany. trouth@gmx.net
authors.forename: Mathias
authors.initials: M
authors.lastname: Wagner
authors.forename: Jens P
authors.initials: JP
authors.lastname: Klussmann
authors.forename: Olaf
authors.initials: O
authors.lastname: Dirsch
authors.forename: Vivienne A
authors.initials: VA
authors.lastname: Rose
authors.forename: Orlando
authors.initials: O
authors.lastname: Guntinas-Lichius
authors.forename: Dirk
authors.initials: D
authors.lastname: Theegarten
authors.forename: Holger
authors.initials: H
authors.lastname: Sudhoff
authors.forename: Roland
authors.initials: R
authors.lastname: Linder
mesh_terms: D000235:Adenolymphoma / Q000473:pathology / Q000821:virology*; D008949:Adenoma, Pleomorphic / Q000473:pathology / Q000821:virology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010306:Parotid Gland / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012463:Saliva / Q000821:virology; D012468:Salivary Gland Neoplasms / Q000473:pathology / Q000821:virology*; D017422:Sequence Analysis, DNA; D013363:Submandibular Gland / Q000473:pathology / Q000821:virology; D022783:Torque teno virus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral