Cell adhesion characteristics of a monocytic cell line derived from rainbow trout, Oncorhynchus mykiss.
In experiments investigating the adhesive properties of the rainbow trout splenic monocyte-like cell line RTS11 it was found that the cells bound with low affinity to plates coated with bovine serum albumin (BSA) but that phorbol ester-induced activation/differentiation greatly increased adhesion to BSA. Similarly, pre-exposure to 500 microM MnCl(2) at time of plating, increased RTS11 adhesion to BSA coated plates, in agreement with the reported ability of divalent cations such as Mn(2+) to activate integrins. Integrins are a diverse family of heterodimeric cell surface glycoproteins that have been shown to mediate cell-cell and cell-extracellular matrix adhesion. Transcripts of the beta(2)-integrin CD18 were detected by PCR in RTS11 but not in RTG-2 cells, a fibroblastic lineage derived from rainbow trout gonads. These results suggest that differentiated RTS11 express molecules related to members of the beta(2)-integrin subfamily such as the macrophage lineage marker Mac-1 (CD11b/CD18) and/or p150,95 (CD11c/CD18) and possibly as well alpha(4)beta(1) of the beta(1)-integrin subfamily.
Reinhart
B
B
Department of Biology, Wilfrid Laurier University, Waterloo, ON, Canada N2L 3C5.
DeWitte-Orr
S J
SJ
Van Es
S J
SJ
Bols
N C
NC
Lee
L E J
LE
D000818:Animals; D018821:CD18 Antigens / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D016167:Integrin alphaXbeta2 / Q000235:genetics / Q000378:metabolism*; D016177:Macrophage-1 Antigen / Q000235:genetics / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D017686:Oncorhynchus mykiss; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D014158:Transcription, Genetic
D018821:CD18 Antigens; D016167:Integrin alphaXbeta2; D016177:Macrophage-1 Antigen; D012333:RNA, Messenger; D012710:Serum Albumin, Bovine; D008345:Manganese