title: Amplification and overexpression of the cyclin D1 gene in head and neck squamous cell carcinoma.
abstract: Aims-To determine cyclin D1 gene amplification and expression levels in head and neck squamous cell carcinoma (HNSCC) patients.Methods-Total RNA and genomic DNA were isolated from 40 samples of HNSCC tissue and matched normal tissue and were hybridised with a cyclin D1 cDNA probe. Northern and Southern analyses were used to detect mRNA overexpression and cyclin D1 gene amplification, respectively.Results-15 of the 40 HNSCC samples examined (38%) showed cyclin D1 gene amplification. Of these 15 samples, all 13 from which RNA was available showed increased cyclin D1 expression.Conclusions-HNSCC patients with both amplification and overexpression of the cyclin D1 gene are at greater risk than patients who showed no cyclin D1 gene amplification; amplification and over-expression of the cyclin D1 gene may play an important role in the progression of HNSCC and in clinical outcome.
authors.affiliation: Department of Cell Biology, Neurobiology and Anatomy, College of Medicine, University of Cincinnati, 231 Bethesda Avenue, Cincinnati, Ohio 45267-0521, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: Z P
authors.initials: ZP
authors.lastname: Pavelic
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Gleich
authors.forename: K
authors.initials: K
authors.lastname: Radack
authors.forename: J L
authors.initials: JL
authors.lastname: Gluckman
authors.forename: P J
authors.initials: PJ
authors.lastname: Stambrook