Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii lambda-carrageenan using carrabiose oligosaccharides prepared by enzymatic hydrolysis.
lambda-Carrageenan extracted from Gigartina skottsbergii tetrasporophyte was completely digested by a purified Pseudoalteromonas carrageenovora lambda-carrageenase. The main digestion products were fractionated and analysed by (1)H and (13)C NMR spectroscopy. All the oligosaccharides observed belong to the neo-carrabiose oligosaccharide series indicating that the lambda-carrageenase cleaves the beta-(1-->4) glycosidic bonds. (1)H and (13)C NMR spectra recorded on oligomers from DP 2 to DP 8 were fully interpreted allowing unambiguous assignment of the lambda-carrageenan spectra. Besides the typical oligo-lambda-carrageenans, we have also characterised a heptasulfated tetrasaccharide which demonstrates the random over-sulfation along the chain of G. skottsbergii lambda-carrageenan.
Guibet
Marion
M
Végétaux Marins et Biomolécules, UMR 7139 (CNRS/UPMC), Station Biologique, Roscoff, France.
Kervarec
Nelly
N
Génicot
Sabine
S
Chevolot
Yann
Y
Helbert
William
W
D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002351:Carrageenan / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D005685:Galactans / Q000032:analysis / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D031161:Pseudoalteromonas / Q000201:enzymology; D000461:Rhodophyta / Q000737:chemistry*
D002247:Carbon Isotopes; D005685:Galactans; D009844:Oligosaccharides; C080061:carrabiose; D002351:Carrageenan; D006026:Glycoside Hydrolases