title: Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells.
abstract: We here provide definitive evidence that ginsenoside-Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol-3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1-induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH-6. Also, knockdown of GR completely eliminated the Rg1-induced NO production. This study revealed that Rg1 can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non-transcriptional PI3K/Akt pathway.
authors.affiliation: Department of Biology, Hong Kong Baptist University, Hong Kong.
authors.forename: Kar Wah
authors.initials: KW
authors.lastname: Leung
authors.forename: Yuen-Kit
authors.initials: YK
authors.lastname: Cheng
authors.forename: Nai Ki
authors.initials: NK
authors.lastname: Mak
authors.forename: Kelvin K C
authors.initials: KK
authors.lastname: Chan
authors.forename: T P David
authors.initials: TP
authors.lastname: Fan
authors.forename: Ricky N S
authors.initials: RN
authors.lastname: Wong
mesh_terms: D002867:Chromones / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D036145:Ginsenosides / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology; D009025:Morpholines / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis*; D052250:Nitric Oxide Synthase Type III / Q000187:drug effects* / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D021381:Protein Transport / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D002867:Chromones; D004791:Enzyme Inhibitors; D036145:Ginsenosides; D009025:Morpholines; D011965:Receptors, Glucocorticoid; D009569:Nitric Oxide; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D015735:Mifepristone; D052250:Nitric Oxide Synthase Type III; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C035054:ginsenoside Rg1