title: Effect of early administration of exogenous basic fibroblast growth factor on acute edematous pancreatitis in rats.
abstract: OBJECTIVE
  To observe the therapeutic effect of early administration of exogenous Basic fibroblast growth factor (bFGF) on acute edematous pancreatitis (AEP) in rats.
  
  
  METHODS
  Thirty male Sprague-Dawley rats were randomly divided into three (n = 10): normal control group (group I), AEP group (group II) and AEP with bFGF treatment group (group III). AEP was induced by subcutaneous injection of cerulein (5.5 microg/kg and 7.5 microg/kg) at 1 h interval into rats of groups II and III. Three hours after induction of AEP, 100 microg/kg bFGF was administrated intraperitoneally for 1 h to group III rats. For test of DNA synthesis in acinar cells, 5-bromo-2'-deoxyuridine (BrdU) labeling solution was intraperitoneally injected into the rats of groups II and III 24 h after bFGF treatment. The changes in serum amylase, lipase, pancreatic tissue wet/dry ratio were detected.
  
  
  RESULTS
  In bFGF treatment group, there was a significant decrease in the volume of serum amylase, lipase and the pancreatic wet/dry weight ratio(1383.0+/-94.6 U/L, 194.0+/-43.6 U/L, 4.32+/-0.32) compared to AEP group (3464+/-223.7 U/L, 456+/-68.7 U/L, 6.89+/-0.47) (P < 0.01), and no significant difference was found between bFGF treatment and control group (1289+/-94.0 U/L, 171+/-23.4 U/L, 4.12+/-0.26, P > 0.05). The inflammatory changes such as interstitial edema, polymorphonuclear neutrophils (PMNs) and vacuolization were significantly ameliorated compared to AEP group (P < 0.01). A small number of BrdU-labeled nuclei were observed in acinar cells of AEP rats (1.8+/-0.3 nuclei/microscopic field, n = 10) while diffuse BrdU-labeled nuclei were found in bFGF-treated rats (18.9+/-1.4 nuclei/microscopic field, n = 10) (P < 0.01). Immunohistochemical study showed increased DNA synthesis in pancreatic acinar cells.
  
  
  CONCLUSIONS
  Early administration of exogenous bFGF has significant therapeutic effect on cerulein-induced acute edematous pancreatitis in rats. Its mechanism is related to the amelioration of inflammation and facilitation of pancreatic regeneration.
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital, 198 Red Flag Road, Huzhou 313000, Zhejiang Province, China. yanqiang@ppp.huptt.zj.cn
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yan
authors.forename: Xing
authors.initials: X
authors.lastname: Yao
authors.forename: Li-Cheng
authors.initials: LC
authors.lastname: Dai
authors.forename: Guo-Lei
authors.initials: GL
authors.lastname: Zhang
authors.forename: Jin-Liang
authors.initials: JL
authors.lastname: Ping
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: He
authors.forename: Chun-Fan
authors.initials: CF
authors.lastname: Han
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D002108:Ceruletide; D004247:DNA / Q000032:analysis; D018450:Disease Progression; D004487:Edema / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology / Q000627:therapeutic use*; D007150:Immunohistochemistry; D008049:Lipase / Q000097:blood; D008297:Male; D009929:Organ Size; D010179:Pancreas / Q000737:chemistry / Q000473:pathology; D019283:Pancreatitis, Acute Necrotizing / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects
chemical_list: D016222:Fibroblast Growth Factor 2; D002108:Ceruletide; D004247:DNA; D008049:Lipase; D000681:Amylases