title: Virulence factors in enterococcal infections of orthopedic devices.
abstract: Enterococci are opportunistic pathogens which today represent one of the leading causes of nosocomial infections. We have examined a collection of 52 Enterococcus faecalis isolated from orthopedic infections to determine if they were characterized by a specific pattern of virulence factors. The isolates were evaluated for biofilm formation, presence of genes coding the enterococcal surface protein (esp) and gelatinase (gelE), as well as for gelatinase production. While the rate of esp-positive isolates was comparable to that found among strains from other clinical sources, we found a significantly higher rate of strong biofilm formers and gelatinase producers. Particularly high was the rate of gelE-carrying strains expressing the gene. Data suggest that these two factors in particular may play an important role in enterococcal infections associated with biomaterials.
authors.affiliation: Department of Infectious, Parasitic and Immune-mediated Diseases, Higher Institute of Health, Rome, Italy. baldassa@iss.it
authors.forename: L
authors.initials: L
authors.lastname: Baldassarri
authors.forename: R
authors.initials: R
authors.lastname: Creti
authors.forename: S
authors.initials: S
authors.lastname: Recchia
authors.forename: M
authors.initials: M
authors.lastname: Pataracchia
authors.forename: G
authors.initials: G
authors.lastname: Alfarone
authors.forename: G
authors.initials: G
authors.lastname: Orefici
authors.forename: D
authors.initials: D
authors.lastname: Campoccia
authors.forename: L
authors.initials: L
authors.lastname: Montanaro
authors.forename: C R
authors.initials: CR
authors.lastname: Arciola
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D013293:Enterococcus faecalis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D018093:Gelatinases / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis / Q000382:microbiology*; D009983:Orthopedic Equipment / Q000382:microbiology*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors
chemical_list: D037521:Virulence Factors; D018093:Gelatinases