Feruloyl esterases as a tool for the release of phenolic compounds from agro-industrial by-products.
Agro-industrial by-products are a potential source of added-value phenolic acids with promising applications in the food and pharmaceutical industries. Here two purified feruloyl esterases from Aspergillus niger, FAEA and FAEB were tested for their ability to release phenolic acids such as caffeic acid, p-coumaric acid and ferulic acid from coffee pulp, apple marc and wheat straw. Their hydrolysis activity was evaluated and compared with their action on maize bran and sugar beet pulp. The specificity of both enzymes against natural and synthetic substrates was evaluated; particular attention was paid to quinic esters and lignin monomers. The efficiency of both enzymes on model substrates was studied. We show the ability of these enzymes to hydrolyze quinic esters and ester linkages between phenolic acids and lignin monomer.
Benoit
Isabelle
I
UMR-1163 INRA de Biotechnologie des Champignons Filamenteux, IFR86-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
Navarro
David
D
Marnet
Nathalie
N
Rakotomanomana
Nnjara
N
Lesage-Meessen
Laurence
L
Sigoillot
Jean-Claude
JC
Asther
Marcel
M
Asther
Michèle
M
D000383:Agriculture / Q000379:methods*; D016573:Agrochemicals / Q000032:analysis*; D001234:Aspergillus niger / Q000201:enzymology; D001673:Biodegradation, Environmental; D002109:Caffeic Acids / Q000737:chemistry / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003069:Coffee / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D006868:Hydrolysis; D062385:Hydroxybenzoates / Q000737:chemistry / Q000378:metabolism*; D007220:Industrial Waste / Q000032:analysis*; D007527:Isoenzymes / Q000378:metabolism; D027845:Malus / Q000737:chemistry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D010945:Plants, Edible / Q000737:chemistry; D013379:Substrate Specificity; D003313:Zea mays / Q000737:chemistry
D016573:Agrochemicals; D002109:Caffeic Acids; D003069:Coffee; D003373:Coumaric Acids; D005656:Fungal Proteins; D062385:Hydroxybenzoates; D007220:Industrial Waste; D007527:Isoenzymes; D010936:Plant Extracts; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; C017616:phenolic acid; C040048:caffeic acid