title: Membrane lateral diffusion and capture of CFTR within transient confinement zones.
abstract: The cystic fibrosis transmembrane conductance regulator (CFTR) channel interacts with scaffolding and other proteins that are expected to restrict its lateral movement, yet previous studies have reported predominantly free diffusion. We examined the lateral mobility of CFTR channels on live baby hamster kidney cells using three complementary methods. Channels bearing an extracellular biotinylation target sequence were labeled with streptavidin conjugated with fluorescent dyes (Alexa Fluor 488 or 568) or quantum dots (qDot605). Fluorescence recovery after photobleaching and image correlation spectroscopy of the dye-labeled channels revealed a significant immobile population ( approximately 50%), which was confirmed by direct single particle tracking (SPT) of qDot605-labeled CFTR. Adding 10 histidine residues at the C-terminus of CFTR to mask the postsynaptic density 95, Discs large, ZO-1 (PDZ) binding motif abolished its association with EBP50/NHERF1, reduced the immobile fraction, and increased mobility. Other interactions that are not normally detected on this timescale became apparent when binding of PDZ domain proteins was disrupted. SPT revealed that CFTR(His-10) channels diffuse randomly, become immobilized for periods lasting up to 1 min, and in some instances are recaptured at the same location. The impact of transient confinement on the measured diffusion using the three fluorescence techniques were assessed using computer simulations of the biological experiments. Finally, the impact of endosomal CFTR on mobility measurements was assessed by fluorescence correlation spectroscopy. These results reveal unexpected features of CFTR dynamics which may influence its ion channel activity.
authors.affiliation: Department of Physiology, McGill University, Montréal, Québec, Canada H3G 1Y6.
authors.forename: Ian R
authors.initials: IR
authors.lastname: Bates
authors.forename: Benedict
authors.initials: B
authors.lastname: Hébert
authors.forename: Yishan
authors.initials: Y
authors.lastname: Luo
authors.forename: Jie
authors.initials: J
authors.lastname: Liao
authors.forename: Alexia I
authors.initials: AI
authors.lastname: Bachir
authors.forename: David L
authors.initials: DL
authors.lastname: Kolin
authors.forename: Paul W
authors.initials: PW
authors.lastname: Wiseman
authors.forename: John W
authors.initials: JW
authors.lastname: Hanrahan
mesh_terms: D000818:Animals; D001703:Biophysics / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D019731:Carbon-Nitrogen Ligases / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D006224:Cricetinae; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000737:chemistry*; D004058:Diffusion; D029968:Escherichia coli Proteins / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods; D006639:Histidine / Q000737:chemistry; D006801:Humans; D007668:Kidney / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C505032:CFTR protein, human; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D006639:Histidine; D001710:Biotin; D019731:Carbon-Nitrogen Ligases; C058125:birA protein, E coli