title: Determination of cellular localization of expression of flavin-containing monooxygenase genes in mouse tissues by in situ hybridization.
abstract: Methods are described for the cellular localization of expression of flavin-containing monooxygenase (FMO) genes in various mouse tissues by in situ hybridization. These include the production of digoxigenin (DIG)-labeled antisense and sense RNA probes by transcription from FMO cDNA templates, the preparation of paraffin wax-embedded and cryostat tissue sections, the hybridization of RNA probes to tissue sections, and the specific detection of hybridized probes using an antibody to DIG.
authors.affiliation: Department of Biochemistry and Molecular Biology, University College London, UK.
authors.forename: Azara
authors.initials: A
authors.lastname: Janmohamed
authors.forename: lan R
authors.initials: lR
authors.lastname: Phillips
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Shephard
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D018076:DNA, Complementary; D015870:Gene Expression; D017403:In Situ Hybridization; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D051379:Mice; D010105:Oxygenases / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming)