Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro.
Purified avian infectious bronchitis virus (IBV) was used to screen a random phage display peptide library. After the fourth panning, 10 positive phages were sequenced and characterized. The phages specifically inhibited IBV infectivity in HeLa cells and blocked IBV haemagglutination. One linear peptide "GSH HRH VHS PFV" from the positive phages with the highest neutralization titer was synthesized and this peptide inhibited IBV infection in HeLa as well. The results may contribute to development of antiviral therapeutics for IBV and studying the determinants for viral and cell interaction.
Peng
Bo
B
Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Chen
Hanyang
H
Tan
Yadi
Y
Jin
Meilin
M
Chen
Huanchun
H
Guo
Aizhen
A
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics; D003001:Cloning, Molecular; D006367:HeLa Cells; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D001351:Infectious bronchitis virus / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D009842:Oligopeptides / Q000138:chemical synthesis / Q000235:genetics / Q000494:pharmacology*; D019151:Peptide Library; D017386:Sequence Homology, Amino Acid
D009842:Oligopeptides; D019151:Peptide Library