Cellulose acetate 1,2-benzenedicarboxylate inhibits infection by cell-free and cell-associated primary HIV-1 isolates.
Cellulose acetate 1,2-benzenedicarboxylate (CAP), a pharmaceutical excipient used for enteric film coating of capsules and tablets, was previously shown to have potent inhibitory activity against infection by human immunodeficiency virus type 1 (HIV-1) T cell line-adapted (TCLA) strains. In the present study, we determined the inhibitory activity of CAP against infection by cell-free and cell-associated primary HIV-1 isolates with distinct genotypes and biotypes in cervical explants, peripheral blood mononuclear cells (PBMCs), monocytederived macrophages (MDMs), and CEMx174 5.25M7 cells. CAP blocked infection by cell-free and cell-associated HIV-1 in cervical explants. It inhibited infection by cell-free primary HIV-1 isolates (clades A to G and group O) in PBMCs, MDMs, and CEMx174 5.25M7 cells and blocked transmissions of the cell-associated primary HIV-1 isolates from dendritic cells (DCs) to PBMCs, from MDMs to PBMCs, and from PBMCs to CEMx174 5.25M7 cells. The inhibitory activity of CAP on infection by the cell-free and cell-associated primary HIV-1 isolates is independent of viral subtypes and coreceptor usage. These data suggest that CAP is a good microbicide candidate that can be further developed for preventing sexual transmission of HIV-1.
Lu
Hong
H
Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York 10021, USA.
Zhao
Qian
Q
Wallace
Greg
G
Liu
Shuwen
S
He
Yuxian
Y
Shattock
Robin
R
Neurath
A Robert
AR
Jiang
B Shibo
BS
D019380:Anti-HIV Agents / Q000494:pharmacology*; D002460:Cell Line; D002474:Cell-Free System / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D002478:Cells, Cultured; D002482:Cellulose / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002584:Cervix Uteri / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D005260:Female; D015658:HIV Infections / Q000517:prevention & control* / Q000821:virology; D015497:HIV-1 / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000821:virology
D019380:Anti-HIV Agents; D002482:Cellulose