Effect of DNA repair protein Rad18 on viral infection.
Host factors belonging to the DNA repair machineries are assumed to aid retroviruses in the obligatory step of integration. Here we describe the effect of DNA repair molecule Rad18, a component of the post-replication repair pathway, on viral infection. Contrary to our expectations, cells lacking Rad18 were consistently more permissive to viral transduction as compared to Rad18(+/+) controls. Remarkably, such susceptibility was integration independent, since retroviruses devoid of integration activity also showed enhancement of the initial steps of infection. Moreover, the elevated sensitivity of the Rad18(-/-) cells was also observed with adenovirus. These data indicate that Rad18 suppresses viral infection in a non-specific fashion, probably by targeting incoming DNA. Furthermore, considering data published recently, it appears that the interactions between DNA repair components with incoming viruses, often result in inhibition of the infection rather than cooperation toward its establishment.
Lloyd
Aliza G
AG
Aaron Diamond AIDS Research Center, The Rockefeller University, New York, New York, USA.
Tateishi
Satoshi
S
Bieniasz
Paul D
PD
Muesing
Mark A
MA
Yamaizumi
Masaru
M
Mulder
Lubbertus C F
LC
D000257:Adenoviridae Infections / Q000517:prevention & control*; D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000378:metabolism*; D004198:Disease Susceptibility; D006801:Humans; D019426:Integrases / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D012190:Retroviridae / Q000235:genetics / Q000502:physiology; D012192:Retroviridae Infections / Q000517:prevention & control*; D016662:Virus Integration
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C485953:Rad18 protein, mouse; D019426:Integrases