title: Determination of protein oxidation by mass spectrometry and method transfer to quality control.
abstract: Characterization and quantitative analysis of oxidation plays an important role in biopharmaceutical development. This study demonstrates an approach to the assessment of susceptible to oxidation methionine residues in monoclonal antibodies and recombinant proteins. A method for the determination of oxidation levels by peptide mapping with mass spectrometric (MS) detection is described and its advantages compared to the UV detection are presented. Good linearity and reproducibility for determination of oxidation with MS detection are demonstrated (R2 > 0.99; RSDs of 4-9%). Aspects of method transfer to quality control group (QC) are discussed. As well, a quick and easy flow injection/MS method is proposed to substitute for peptide map analysis. Peptide coverage, linearity, reproducibility, robustness, sensitivity and quantitative oxidation results are compared for the flow injection/MS and LC/MS approaches.
authors.affiliation: Biogen Idec Inc., Cambridge, MA, USA.
authors.forename: Damian
authors.initials: D
authors.lastname: Houde
authors.forename: Paivi
authors.initials: P
authors.lastname: Kauppinen
authors.forename: Rohin
authors.initials: R
authors.lastname: Mhatre
authors.forename: Yelena
authors.initials: Y
authors.lastname: Lyubarskaya
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006861:Hydrogen Peroxide / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008715:Methionine / Q000737:chemistry*; D010084:Oxidation-Reduction; D010449:Peptide Mapping; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D011786:Quality Control; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012697:Serine Endopeptidases / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D011506:Proteins; D011993:Recombinant Fusion Proteins; D008715:Methionine; D006861:Hydrogen Peroxide; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase