title: Periodically interrupted amperometry. A way of improving analytical performance of membrane coated electrodes.
abstract: Amperometry is a powerful voltammetric measuring method. Its application is specially advantageous when used in combination with a separation step or with some other sample treatment method providing selectivity. The selectivity is often achieved by coating the amperometric working electrode surface with a membrane of special character. Size exclusion membrane, immobilized enzyme containing reaction layer, protecting dialysis membrane, perm selective ion exchange film etc can be mentioned here. In conventional amperometry the measuring potential is continuously applied, therefore in case of membrane coated electrodes the electrode process depletes the diffusion layer. In this work the performance of a new periodically interrupted amperometric (PIA) measuring program has been investigated in case of glucose enzyme sensor. The measuring program allowing time for reloading the diffusion layer provided higher current and therefore improved sensitivity and lower limit of detection.
authors.affiliation: Research Group for Chemical Sensors of the Hungarian Academy of Sciences, University of Pécs, Ifjúság u. 6. H-7601, Hungary. lnagy@ttk.pte.hu
authors.forename: Livia
authors.initials: L
authors.lastname: Nagy
authors.forename: Nikoletta
authors.initials: N
authors.lastname: Kálmán
authors.forename: Géza
authors.initials: G
authors.lastname: Nagy
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase; D006861:Hydrogen Peroxide; D008567:Membranes, Artificial; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose