Iron uptake and release by macrophages is sensitive to propranolol.
In this study we have tested the effects of d-propranolol (D-Pro) on the iron uptake, iron release and oxidative response of iron-loaded cells in a cellular model of iron-overload using isolated rat peritoneal macrophages incubated with iron-dextran (Fe-D). Pretreatment of macrophages with D-Pro (5-200 microM) prior to Fe-D exposure decreased the cellular iron content and partially prevented iron release from latex-activated macrophages. Release of reactive oxygen species from activated cells was detected by dichlorodihydrofluorescein (DCDHF, 5 microM) oxidation. We found that loading cells with Fe-D increased their response to latex, which was prevented by the lysosomotropic antioxidant agent D-Pro (10 microM).
Komarov
Andrei M
AM
Division of Experimental Medicine, Departments of Biochemistry & Molecular Biology, The George Washington University Medical Center, 2300 Eye St., NW, Ross Hall, Rm. 441A, Washington, DC 20037, USA. phyamk@gwumc.edu
Hall
Jonathon M
JM
Chmielinska
Joanna J
JJ
Weglicki
William B
WB
D000818:Animals; D004305:Dose-Response Relationship, Drug; D007501:Iron / Q000378:metabolism*; D007505:Iron-Dextran Complex / Q000378:metabolism; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D011433:Propranolol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; D007505:Iron-Dextran Complex; D011433:Propranolol; D007501:Iron