The haemoxisome: a haem-iron containing structure in the Rhodnius prolixus midgut cells.
Rhodnius prolixus midgut was analysed using transmission electron microscopy and electron spectroscopic imaging in order to localize the cellular structures involved in haem metabolism. In the posterior midgut, special cellular electron-dense structures were observed. These structures are here designated haemoxisomes. Haemoxisomes are present in the epithelial cells at various time points after a blood meal. Several days after the blood meal, some of them become less electron-dense. By electron spectroscopic imaging, large amounts of iron and oxygen were detected in these cellular structures. The iron is probably bound to the porphyrin ring as an iron-protoporphyrin IX complex, as detected using the diaminobenzidine technique. An interesting observation was the presence of endoplasmic reticulum surrounding the haemoxisomes during some special periods. Iron content was monitored in the posterior midgut epithelium and was found to be constant at the initial days after a blood meal, but slightly higher at the end of the digestive process (from 13th up to 20th day). These results are in agreement with the observation that the appearance of the haemoxisomes changes at the end of the digestive process. The ability to degrade haem seems to depend on the presence of endoplasmic reticulum as observed using a haem degradation assay in the presence of an endoplasmic reticulum-enriched fraction. Taken together these results suggest that haemoxisomes may play a role in intracellular haem detoxification.
Silva
José Roberto
JR
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego 2000, Campos dos Goytacazes, Rio de Janeiro, RJ 28015-620, Brazil.
Gomes-Silva
Leonardo
L
Lins
Ulysses Casado
UC
Nogueira
Nadir F S
NF
Dansa-Petretski
Marílvia
M
D000818:Animals; D001769:Blood / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism*; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000648:ultrastructure*; D006418:Heme / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D046032:Microscopy, Energy-Filtering Transmission Electron; D012239:Rhodnius / Q000378:metabolism / Q000648:ultrastructure*
D006418:Heme; D007501:Iron