title: Studies on the luteolytic, oestrogenic and follicle-stimulating hormone like activity of plant Rhaphidophora pertusa (Roxb.).
abstract: The phytochemical, pharmacological and toxicological feature of plant Rhaphidophora pertusa (Roxb.) was done. Phytosteroids, flavonoids, tannins and glucosides were detected in the plant extracts. In cross-bred (Zebu X Holstein-Friesian or Jersey) dairy cows, subsequent to prostaglandin (PG) induced oestrus, to each group (n=4), cloprostenol (PG control) 100 microg i.m. on day 10, the rice gruel (vehicle) was fed on day 10 or the fresh stem (1 kg/animal/day) in rice gruel on day 9, or days 9 and 10, or days 9-11 of the oestrous cycle. Each group received subcutaneously either 5% gum acacia suspension or the plant ethyl acetate or methanol extract (1g in gum acacia) on days 8 (to bannur ewes) or 10 (to dairy cows) of the oestrous cycle. In PG control cows or ewes, there was induction of oestrus in 48 h and a fall in serum progesterone concentration. The feeding of fresh stem in the rice gruel or the s.c. administration of the plant extract did not induce oestrus or significantly (P>0.05) alter the serum progesterone, bilirubin, calcium, creatinine, phosphorus, magnesium and glucose concentrations or the total erythrocyte and leucocyte count, differential leucocyte count and haemoglobin concentration. The plant did not cause any toxicity in the cow or ewe. In immature rats, the aqueous or methanol (hot or cold) extract did not cause any follicle-stimulating hormone (FSH)-like activity. The methanol extract increased the uterine weight in ovariectomised rats. This suggested the presence of oestrogenic activity in the plant. In conclusion, the present study revealed the presence of oestrogenic activity in the plant and the absence of luteolytic or FSH-like or toxic activity.
authors.affiliation: Department of Pharmacology and Toxicology, Veterinary College, Hebbal, Bangalore 560024, India.
authors.forename: C R
authors.initials: CR
authors.lastname: Santhosh
authors.forename: N B
authors.initials: NB
authors.lastname: Shridhar
authors.forename: K
authors.initials: K
authors.lastname: Narayana
authors.forename: S G
authors.initials: SG
authors.lastname: Ramachandra
authors.forename: S
authors.initials: S
authors.lastname: Dinesh
mesh_terms: D000818:Animals; D029064:Araceae; D002417:Cattle; D003338:Corpus Luteum / Q000187:drug effects; D030762:Estrous Cycle / Q000187:drug effects*; D004973:Estrus Synchronization; D005260:Female; D003341:Luteolysis / Q000187:drug effects; D008186:Luteolytic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D009929:Organ Size / Q000187:drug effects; D006080:Ovarian Follicle / Q000187:drug effects; D010052:Ovariectomy; D048789:Phytoestrogens / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology; D018547:Plant Stems; D011374:Progesterone / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects*; D012756:Sheep; D014599:Uterus / Q000187:drug effects / Q000254:growth & development
chemical_list: D008186:Luteolytic Agents; D048789:Phytoestrogens; D010936:Plant Extracts; D011374:Progesterone