Quantitative GC-ECD analysis of halogenated metabolites: determination of surface and within-thallus elatol of Laurencia obtusa.
Information on natural concentrations or variability of secondary metabolites in marine organisms may be important both to ecological/evolutionary and applied approaches. A gas chromatographic procedure with an electron capture detector (GC-ECD) was developed to quantify the sesquiterpenoid elatol at the surface and within-thalli of 70 specimens of the red seaweed Laurencia obtusa. The concentration of elatol was highest within-thalli [9.89 mg g(-1) of L. obtusa, dry weight (d.w.)], compared to lower values found at the surface [0.006 mg g(-1) of L.obtusa (d.w.), or 0.5-10.0 ng cm(-2)]. This method provides a rapid and inexpensive quantification of small quantities of elatol, and probably may also be used to quantify other halogenated compounds usually found in red seaweeds.
Sudatti
Daniela Bueno
DB
Departamento de Biologia Marinha, Universidade Federal Fluminense, Caixa Postal 100644, CEP 24001-970 Niterói, Rio de Janeiro, Brazil.
Rodrigues
Silvana Vianna
SV
Pereira
Renato Crespo
RC
D002849:Chromatography, Gas; D044722:Laurencia / Q000737:chemistry* / Q000378:metabolism; D013141:Spiro Compounds / Q000032:analysis* / Q000378:metabolism
D013141:Spiro Compounds; C485576:elatol