Synthesis and characterization of novel biotinylated biodegradable poly(ethylene glycol)-b-poly(carbonate-lactic acid) copolymers.
Poly(ethylene glycol)-b-poly(5-benzyloxy-trimethylene carbonate-lactic acid) copolymers (PEG-b-P(BTMC-LA)) were synthesized by ring-opening polymerization of lactide and 5-benzyloxy trimethylene carbonate in the presence of mono-hydroxyl poly(ethylene glycol) with diethyl zinc as catalyst. They were further converted into deprotected copolymers with the pendant hydroxyl groups by hydrogenolysis in the presence of Pd(OH)2/C, and finally conjugated with biotin through the free hydroxyl groups. Gel permeation chromatography, Fourier transform infrared, differential scanning calorimetry and 1H nuclear magnetic resonance studies confirmed the copolymer structures and successful attachment of biotin to the copolymer.
Xie
Zhigang
Z
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Guan
Huili
H
Lü
Changhai
C
Chen
Xuesi
X
Jing
Xiabin
X
D020341:Absorbable Implants; D000042:Absorption; D001360:Avidin / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D008422:Materials Testing; D008968:Molecular Conformation; D011091:Polyesters / Q000032:analysis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000032:analysis / Q000737:chemistry*
D001672:Biocompatible Materials; D011091:Polyesters; C511487:poly(ethylene glycol)-block-poly(5-benzyloxy-trimethylene carbonate-lactic acid); D001360:Avidin; D011092:Polyethylene Glycols; D001710:Biotin