Isolation from canned foods of a novel Thermoanaerobacter species phylogenetically related to Thermoanaerobacter mathranii (Larsen 1997): emendation of the species description and proposal of Thermoanaerobacter mathranii subsp. Alimentarius subsp. Nov.
Several anaerobic, thermophilic, Gram-positive bacteria were isolated from dairy products and canned meats. While some isolates were identified as Thermoanaerobacter thermohydrosulfuricus, comparisons of 16S rDNA genes indicated that others were phylogenetically closely related to Thermoanaerobacter mathranii, and more distantly related to Thermoanaerobacter thermocopriae and Thermoanaerobacter italicus. Biochemical characteristics, phylogenetic analysis, G+C content, and DNA-DNA hybridization experiments demonstrated that the strains AIP 504.99, AIP 505.99T and AIP 431.03, notwithstanding their high sequence similarities differ from T. mathranii and represent a novel T. mathranii subspecies for which the name T. mathranii subsp. alimentarius is proposed. The type strain is strain AIP 505.99T = CIP 108280T = CCUG 49566T. Emendation of the species description for T. mathranii is proposed to include this subspecies.
Carlier
Jean-Philippe
JP
Centre National de Référence des Bactéries Anaérobies et du Botulisme 25 rue du Dr Roux, F-75724 Paris Cedex 15, France. jcarlier@pasteur.fr
Bonne
Isabelle
I
Bedora-Faure
Marie
M
D001482:Base Composition; D043302:Cultured Milk Products / Q000382:microbiology; D015342:DNA Probes; D004269:DNA, Bacterial; D005516:Food Microbiology; D005519:Food Preservation; D008460:Meat / Q000382:microbiology; D010802:Phylogeny; D046968:Thermoanaerobacter / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology
D015342:DNA Probes; D004269:DNA, Bacterial