Analysis of mosquito bloodmeals using RFLP markers.
An important variable in the amplification of arthropod vector-borne diseases is the degree of contact between human hosts and mosquito vectors. To analyze this interaction, a DNA based method was developed to differentiate human bloodmeals from other sources in the mosquito Anopheles stephensi (Diptera: Culicidae) Liston. A portion of the host mitochondrial DNA cytochrome B genes were PCR amplified and classified to the species level based on their restriction fragment length polymorphism (RFLP). The cytochrome B sequences showed sufficient interspecific polymorphism to distinguish between human, cow, sheep, chicken, and guinea pig hosts. XhoI could distinguish human from other vertebrates whereas TaqI alone could separate the others. The importance of these results in epidemiological studies of malaria and other vector borne diseases is discussed.
Oshaghi
Mohammad Ali
MA
Department of Medical Entomology, School of Public Health and Institute of Health Researches, Tehran University of Medical Sciences, P.O.Box 14155-6446, Tehran, Iran. oshaghima@gmail.com
Chavshin
Ali Reza
AR
Vatandoost
Hassan
H
D000818:Animals; D000852:Anopheles / Q000502:physiology*; D001483:Base Sequence; D001769:Blood; D002417:Cattle / Q000097:blood* / Q000235:genetics; D002645:Chickens / Q000097:blood* / Q000235:genetics; D045303:Cytochromes b / Q000737:chemistry / Q000235:genetics; D004247:DNA / Q000097:blood / Q000737:chemistry; D005260:Female; D006168:Guinea Pigs / Q000097:blood* / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000097:blood* / Q000235:genetics; D013045:Species Specificity
D004247:DNA; D045303:Cytochromes b