G1/S Cyclins interact with regulatory subunit of PKA via A-kinase anchoring protein, AKAP95.
The mechanisms by which cyclins promote mammalian cell cycle progression have been a topic of intense investigation over the last decade. We previously described an interaction between D-type cyclins and A-kinase anchoring protein, AKAP95. Here, we demonstrate that AKAP95 can also bind cyclin E1. Association between AKAP95 and cyclins is displaced by CDKs. We show that these G(1)/S cyclins can interact with RII subunit of PKAalpha through AKAP95. The presence of alternate complexes cyclin-CDK and cyclin D/E-AKAP95-PKA.RIIalpha suggest different roles of G(1)/S cyclins and a wider biological importance of these interactions in cells.
Arsenijevic
Tatjana
T
Institute of Interdisciplinary Research (IRIBHM), and Laboratoire de Chimie Biologique et de la Nutrition, School of Medicine, Free University of Brussels, Campus Erasme, Brussels, Belgium. tarsenij@ulb.ac.be
Degraef
Chantal
C
Dumont
Jacques E
JE
Roger
Pierre P
PP
Pirson
Isabelle
I
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D056741:Cyclin D; D019927:Cyclin E / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism*; D004285:Dogs; D016193:G1 Phase; D006801:Humans; D011485:Protein Binding; D016196:S Phase; D014162:Transfection
D054758:A Kinase Anchor Proteins; C485099:AKAP8 protein, human; D048868:Adaptor Proteins, Signal Transducing; D056741:Cyclin D; D019927:Cyclin E; D016213:Cyclins; D017868:Cyclic AMP-Dependent Protein Kinases; D018844:Cyclin-Dependent Kinases