title: A new simple HPLC method for measuring mitotane and its two principal metabolites Tests in animals and mitotane-treated patients.
abstract: A new C18 reversed-phase column and UV HPLC method for the detection of mitotane, its principal metabolites, dichlorodiphenylacetate and dichlrodiphenylethene, and its precursor DDT is described. In this article mitotane, dichlorodiphenylacetate, and dichlrodiphenylethene concentrations in organs of rats fed on a mitotane diet, and the effects of erythromycin and grapefruit juice as cytochrome P450 common inhibitors are presented. Tissue accumulation of mitotane and dichlrodiphenylethene, the acquired ability to eliminate dichlorodiphenylacetate, and inhibition of beta-hydroxylation by both inhibitors are illustrated here. Blood samples from mitotane-treated patients revealed two correlations: plasma mitotane/dichlrodiphenylethene and plasma mitotane/red cell mitotane.
authors.affiliation: Department of Clinical and Biological Sciences, Laboratory of Pharmacology, University of Turin, Ospedale San Luigi, Regione Gonzole 10, 10043 Orbassano (TO), Italy. silvia.defrancia@unito.it
authors.forename: Sivia
authors.initials: S
authors.lastname: De Francia
authors.forename: Elisa
authors.initials: E
authors.lastname: Pirro
authors.forename: Franco
authors.initials: F
authors.lastname: Zappia
authors.forename: Francesca
authors.initials: F
authors.lastname: De Martino
authors.forename: Andrea Elio
authors.initials: AE
authors.lastname: Sprio
authors.forename: Fulvia
authors.initials: F
authors.lastname: Daffara
authors.forename: Massimo
authors.initials: M
authors.lastname: Terzolo
authors.forename: Alfredo
authors.initials: A
authors.lastname: Berruti
authors.forename: Francesco
authors.initials: F
authors.lastname: Di Carlo
authors.forename: Franco
authors.initials: F
authors.lastname: Ghezzo
mesh_terms: D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000008:administration & dosage / Q000378:metabolism* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D008939:Mitotane / Q000008:administration & dosage / Q000378:metabolism* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet
chemical_list: D018931:Antineoplastic Agents, Hormonal; D008939:Mitotane