Protective effect of Rosa laevigata against amyloid beta peptide-induced oxidative stress.
The amyloid beta (Abeta) peptide is known to increase free radical production in nerve cells, leading to cell death. To investigate the effect of Rosa laevigata against Abeta-induced oxidative damage, in vitro assays and in vivo behavioral tests were performed. R. laevigata showed cell protective effects against oxidative stress-induced cytotoxicity. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction assay exhibited significant increase in cell viability when rat pheochromocytoma (PC 12) cells were treated with R. laevigata extracts. Administration of R. laevigata extracts to mice significantly reversed the Abeta-induced learning and memory impairment in in vivo behavioral tests. These results suggest that R. laevigata extracts can reduce the cytotoxicity of Abeta in PC 12 cells, possibly by the reduction of oxidative stress, and these extracts may be useful in the prevention of Alzheimer's disease.
Choi
Soo Jung
SJ
Department of Food Technology, Korea University, Seongbuk-gu, Seoul, 136-701, Republic of Korea.
Kim
Mi Jeong
MJ
Heo
Ho Jin
HJ
Kim
Hye Kyung
HK
Hong
Bumshik
B
Kim
Chang-Ju
CJ
Kim
Byung-Gee
BG
Shin
Dong Hoon
DH
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D010795:Phthalic Acids / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D027826:Rosa
D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010795:Phthalic Acids; D010936:Plant Extracts; C019174:dinonylphthalate