title: Effects of various rinsing protocols after the use of amine fluoride/stannous fluoride toothpaste on the acid production of dental plaque and tongue flora.
abstract: The aim of this clinical study was to evaluate the effect of various rinsing protocols on oral acid production 6 h after tooth brushing with an amine fluoride/stannous fluoride (AmF/SnF2) toothpaste. After a 14-day period of using F-free toothpaste, 30 participants followed three experimental protocols each, followed by F-free washout periods in a randomized crossover trial. They used AmF/SnF2 toothpaste twice daily for 1 week, and after brushing, they either rinsed with tap water, omitted the post-brush rinse, or rinsed with an AmF/SnF2 mouthwash. In the F-free washout periods, the participants brushed their teeth without further instructions. Six hours after the last brushing (+/-rinsing) of each period, subjects rinsed with 10 ml 10% sucrose solution for 2 min. A tongue film sample and a buccal plaque sample were taken 4 and 8 min after the sucrose challenge, respectively. Metabolic acid ions were determined by capillary electrophoresis. The results show that (1) omitting the post-brush water rinse did not reduce the production of lactic, acetic or minor acids in plaque, nor on the tongue, and that (2) the additional use of AmF/SnF2 mouthwash after brushing reduced the acid production in plaque and tongue samples for at least 6 h. The distributions of acids produced in the plaque or tongue samples were not statistically different between experimental periods. It is concluded that an increase in the antimetabolic effect of AmF/SnF2 toothpaste in between two daily brushing exercises is not achieved by omitting the post-brush water rinse. The additional use of AmF/SnF2 mouthwash after brushing is effective in reducing the acid metabolism in dental plaque and tongue flora.
authors.affiliation: Department of Cariology, Endodontology, Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. v.gerardu@acta.nl
authors.forename: V A M
authors.initials: VA
authors.lastname: Gerardu
authors.forename: C
authors.initials: C
authors.lastname: van Loveren
authors.forename: M
authors.initials: M
authors.lastname: Heijnsbroek
authors.forename: M J
authors.initials: MJ
authors.lastname: Buijs
authors.forename: G A
authors.initials: GA
authors.lastname: van der Weijden
authors.forename: J M
authors.initials: JM
authors.lastname: ten Cate
mesh_terms: D000143:Acids / Q000032:analysis; D000328:Adult; D002327:Cariostatic Agents / Q000627:therapeutic use*; D016009:Chi-Square Distribution; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000378:metabolism*; D005260:Female; D005459:Fluorides / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D009067:Mouthwashes / Q000737:chemistry / Q000627:therapeutic use; D013395:Sucrose / Q000008:administration & dosage; D014059:Tongue / Q000382:microbiology*; D014099:Toothbrushing; D014100:Toothpastes / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D000143:Acids; D002327:Cariostatic Agents; D009067:Mouthwashes; D014100:Toothpastes; D013395:Sucrose; D005459:Fluorides