title: [Determination of acetylharpagide in Ajuga decumbens by HPLC].
abstract: OBJECTIVE
  To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens.
  
  
  METHODS
  The chromatographic conditions were as follows: a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (15:85), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm.
  
  
  RESULTS
  Linearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively.
  
  
  CONCLUSIONS
  The contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.
authors.affiliation: Institue of Chinese Materia Medica, China Academy of Chinese Medicial Sciences, Bejing 100700, China.
authors.forename: Dang-Kun
authors.initials: DK
authors.lastname: Yue
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Ding
authors.forename: Wei-Hao
authors.initials: WH
authors.lastname: Wang
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Tong
mesh_terms: D031328:Ajuga / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011714:Pyrans / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004365:Drugs, Chinese Herbal; D011714:Pyrans; C077626:8-O-acetylharpagide