[Human fetal ventricular cardiomyocytes in vitro: proliferation and differentiation].
In this study, in the primary cell culture of human fetal cardiomyocytes proliferation of myocytes combines with their differentiation. The cells were isolated enzymatically from 19-22 week-old human fetuses and cultured for 14 days. DNA synthesis, ultrastructure and presence of atrial natriuretic peptide (ANP) were examined. In 7 day-old culture, the myocytes make about 60%, in 14 day-old culture--about 50%. Myocytes synthesize DNA and divide mitotically. After a 24 h incubation with 3H-thymidine in 7 day-old culture 1.8 +/- 0.5% of muscle and 25.2 +/- 11.7% of non-muscle cells are labeled, in 14 day-old culture--2.5 +/- 0.5 and 8.1 +/- 1.7% of cells are labeled, respectively. In 7 and 14 day-old cultures the degree of redifferentiation of contractile apparatus in myocytes varies from scattered actin and myosin filaments surrounded by ribosomes to differentiating myofibrils with distinct sarcomeres and Z-discs. Single electron-dense granules, morphologically similar to secretory atrial granules, display ANP-immunoreactivity. Thus, human fetal ventricular cardiomyocytes in cell culture proliferate, differentiate and synthesize ANP for 14 days; this is indicative of vitality of these cells.
Erokhina
I L
IL
Semenova
E G
EG
Emel'ianova
O I
OI
D009320:Atrial Natriuretic Factor / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005318:Fetal Heart / Q000166:cytology*; D006321:Heart / Q000196:embryology; D006352:Heart Ventricles / Q000166:cytology; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008938:Mitosis; D032342:Muscle Cells / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D009206:Myocardium / Q000166:cytology*; D013997:Time Factors
D009320:Atrial Natriuretic Factor; D004247:DNA