In vivo and in vitro study of porcine oviductal epithelial cells, cumulus oocyte complexes and granulosa cells: a scanning electron microscopy and inverted microscopy study.
The morphology and structure of porcine oviductal epithelial cells (POEC), cumulus-oocyte complexes (COCs) and granulosa cells (GC) were investigated in vivo and in vitro conditions using scanning electron microscopy (SEM) and inverted microscopy. The POEC contained columnar ciliated cells and spherical shaped non-ciliated cells. Both non- and ciliated cells appeared either in groups or distributing among each other. However, the isolation of cells was observed after culture for 48 h. A total of 921 oocytes from 20 ovaries was isolated resulting in an average of 46 oocytes per ovary. They were round in shape, surrounded by zona pellucida with layers of cumulus cells ranging between 89.16 and 144.68 microm in size. As for COCs, they were classified into 4 types; intact-, multi-, partial-cumulus cell layers and completely denuded oocyte. Interestingly, changes in morphology of COCs with intact and multi-cumulus cell layers were observed in the in vitro study. The GCs in the follicular fluid were also round in shape and found as clusters. After culturing in in vitro for 48 h, no change in morphology was observed. The GC appeared in smaller clusters or were present as single cells and their sizes ranged from 6 to 8 microm. The results obtained from this study allow us to have a better understanding of the morphology and nature of cells under both in vivo and in vitro conditions. This information is also important for the study of their secretions and biochemical compositions, which is of great importance to the use of cells as feeder cells in in vitro fertilization in current studies.
Areekijseree
Mayuva
M
Department of Biology, Faculty of Science, Silpakorn University, Nakorn Pathom 73000, Thailand. Maijackee@yahoo.com
Vejaratpimol
Renu
R
D000818:Animals; D002448:Cell Adhesion; D022081:Cell Surface Extensions; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000648:ultrastructure*; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D006107:Granulosa Cells / Q000648:ultrastructure*; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D009865:Oocytes / Q000648:ultrastructure*; D013552:Swine