Pulsed-field gel electrophoresis: a versatile tool for analysis of fungal genomes. A review.
The separation of chromosome-size DNA molecules by pulsed-field gel electrophoresis (PFGE) has become a well-established technique in recent years. Although it has very wide-ranging applications, it made a real breakthrough for fungal genome analysis. Because of the small size of fungal chromosomes, their investigation was not possible earlier. Different PFGE approaches allowed the separation of DNA molecules larger than 10 megabase pairs in size, and electrophoretic karyotypes for numerous previously genetically uncharacterized fungal species could be established. This review discusses the applicability of these electrophoretic karyotypes for the investigation of genome structure, for strain identification and for species delimitation.
Lukácsi
Gyöngyi
G
Department of Microbiology, Faculty of Sciences, University of Szeged, P.O. Box 533, H-6701 Szeged, Hungary. lgy2@freemail.hu
Takó
M
M
Nyilasi
Ildikó
I
D015825:Chromosomes, Fungal / Q000737:chemistry; D004271:DNA, Fungal / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D005658:Fungi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016681:Genome, Fungal
D004271:DNA, Fungal