Brucella spp. lumazine synthase: a novel adjuvant and antigen delivery system to effectively induce oral immunity.
Brucella lumazine synthase (BLS) has been previously used with success as a delivery system for systemic immunization against murine cysticercosis. We herein determined the usefulness of BLS as a new antigen-delivery system and mucosal-adjuvant using KETc1, one of the peptides of the anti-cysticercosis vaccine. A protection of up to 98% was induced when KETc1 was used as a chimera fused to BLS. Used as adjuvant of KETc1, BLS also induced a high level of protection (79%), which did not significantly differ from that induced by the cholera toxin (74%). KETc1 and BLS administered separately also reduced the parasite load. KETc1 administered orally as a chimera, and to a lesser extent with BLS as adjuvant, elicited IgG and IgA specific antibodies, which were detectable both in fecal extracts and in sera, and increased B and CD4 activated cells. BLS-KETc1 also increased the levels of transcription of TNF-alpha, IL-2 and IFNgamma in Peyer's patches, and in spleen, only increased TNF-alpha was observed. Overall, these results showed that BLS can be used as both an antigen-carrier and as an adjuvant in the design of new oral subunit vaccines.
Rosas
Gabriela
G
Facultad de Medicina, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, Morelos, México.
Fragoso
Gladis
G
Ainciart
Natalia
N
Esquivel-Guadarrama
Fernando
F
Santana
Angélica
A
Bobes
Raúl J
RJ
Ramírez-Pliego
Oscar
O
Toledo
Andrea
A
Cruz-Revilla
Carmen
C
Meneses
Gabriela
G
Berguer
Paula
P
Goldbaum
Fernando A
FA
Sciutto
Edda
E
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000284:Administration, Oral; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D002002:Brucella / Q000201:enzymology*; D003551:Cysticercosis / Q000276:immunology / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D018928:Immunity, Mucosal; D051379:Mice; D008807:Mice, Inbred BALB C; D009097:Multienzyme Complexes; D010581:Peyer's Patches / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology; D013621:Taenia / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000276:immunology
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C044822:6,7-dimethyl-8-ribityllumazine synthase