A transgenic rice seed accumulating an anti-hypertensive peptide reduces the blood pressure of spontaneously hypertensive rats.
RPLKPW is a potent anti-hypertensive peptide designed according to the structure of ovokinin(2-7) (RADHPF). In this study, we generated transgenic rice plants that accumulate the RPLKPW peptide as a fusion protein with the rice storage protein glutelin. The engineered peptide is expressed under the control of endosperm-specific glutelin promoters and specifically accumulates in seeds. Oral administration of either the RPLKPW-glutelin fraction or transgenic rice seeds to spontaneously hypertensive rats (SHRs) significantly reduced systolic blood pressures. These results suggest the possible application of transgenic rice seed as a nutraceutical delivery system and specifically for administration of active peptides in hypertension.
Yang
Lijun
L
Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki 305-8602, Japan.
Tada
Yoshifumi
Y
Yamamoto
Masayuki P
MP
Zhao
Hui
H
Yoshikawa
Masaaki
M
Takaiwa
Fumio
F
D000595:Amino Acid Sequence; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage*; D001483:Base Sequence; D001794:Blood Pressure / Q000187:drug effects; D002458:Cell Fractionation; D005822:Genetic Vectors / Q000235:genetics; D006973:Hypertension / Q000188:drug therapy*; D008297:Male; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000008:administration & dosage* / Q000096:biosynthesis* / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010047:Ovalbumin / Q000737:chemistry / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D051381:Rats; D011918:Rats, Inbred SHR; D012639:Seeds / Q000235:genetics / Q000378:metabolism*
D000959:Antihypertensive Agents; C511871:Arg-Pro-Leu-Lys-Pro-Trp; D009842:Oligopeptides; D010446:Peptide Fragments; C119914:ovalbumin (359-364); D010047:Ovalbumin