Infrared imaging microscopy of bone: illustrations from a mouse model of Fabry disease.
Bone is a complex tissue whose composition and properties vary with age, sex, diet, tissue type, health and disease. In this review, we demonstrate how infrared spectroscopy and infrared spectroscopic imaging can be applied to the study of these variations. A specific example of mice with Fabry disease (a lipid storage disease) is presented in which it is demonstrated that the bones of these young animals, while showing typical spatial variation in mineral content, mineral crystal size, and collagen maturity, do not differ from the bones of age- and sex-matched wild type animals.
Boskey
Adele L
AL
Hospital for Special Surgery and Weill Medical College of Cornell University, USA. boskeya@hss.edu
Goldberg
Michel
M
Kulkarni
Ashok
A
Gomez
Santiago
S
D000818:Animals; D001842:Bone and Bones / Q000737:chemistry* / Q000648:ultrastructure*; D003952:Diagnostic Imaging / Q000379:methods*; D000795:Fabry Disease / Q000175:diagnosis*; D051379:Mice; D013055:Spectrophotometry, Infrared