title: [Synthetic and photo-immobilization of photo-active tumor necrosis factor-alpha].
abstract: Tumor Necrosis Factor is one of the cell factors with much stronger anti-tumor activity. In this study, photoactive arylazide-4-azidobenzoic acid was modified to Tumor Necrosis Factor-alpha (TNF-alpha). The IR data (2127cm(-1)) was given to confirm the modification. By photo-immobilization, this modified TNF-a was immobilized on cell culture polystyrene. Both the relation between the amount of TNF-alpha in feed and immobilized TNF-alpha and the influence of different UV irradiation time on photo-immobilization are discussed. Microscopic observations of the photo-immobilization TNF-alpha were made by use of scanning electrom microscope and atomic force microscope.
authors.affiliation: Biotechnology Research Institute, College of Life Science, South China Normal University, Guangzhou 510631, China. gyq69@163.com
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Guan
authors.forename: Huizhou
authors.initials: H
authors.lastname: Zhong
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.forename: Tianhong
authors.initials: T
authors.lastname: Zhou
mesh_terms: D005260:Female; D006801:Humans; D010777:Photochemistry; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry* / Q000627:therapeutic use; D014466:Ultraviolet Rays; D002583:Uterine Cervical Neoplasms / Q000628:therapy*
chemical_list: D014409:Tumor Necrosis Factor-alpha