Length of the linker and the interval between immunizations influences the efficacy of Vibrio cholerae O1, Ogawa hexasaccharide neoglycoconjugates.
Ogawa hexasaccharide neoglycoconjugates induce protective antibodies in mice. Similar Ogawa conjugates but with a longer linker that connects the carrier to shorter saccharides are immunogenic, but generally ineffective at inducing vibriocidal or protective antibodies. The efficacy of Ogawa hexasaccharide neoglycoconjugates of different linker lengths were tested. The majority of mice given immunizations separated by a 14-day gap did not produce vibriocidal or protective antibodies. Mice immunized 28 days apart with immunogens containing the shortest or medium length linker, but not the longest, produced vibriocidal and protective antibodies. A nonprotective, priming dose of purified Ogawa LPS followed 5 days later with a booster of the Ogawa neoglycoconjugates (di-, tetra-, or hexasaccharide) resulted in vibriocidal antibodies at day 10.
Saksena
Rina
R
National Institutes of Health, NIDDK, Laboratory of Medicinal Chemistry, Bethesda, MD, USA.
Ma
Xingquan
X
Wade
Terri K
TK
Kovác
Pavol
P
Wade
William F
WF
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002240:Carbohydrate Sequence; D022121:Cholera Vaccines / Q000737:chemistry / Q000276:immunology*; D005260:Female; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology; D007156:Immunologic Memory; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry / Q000276:immunology*; D011247:Pregnancy; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D013329:Structure-Activity Relationship; D041241:Vibrio cholerae O1 / Q000276:immunology*
D000907:Antibodies, Bacterial; D022121:Cholera Vaccines; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D019081:O Antigens; D012710:Serum Albumin, Bovine