Expressed sequence tag analysis of adult Clonorchis sinensis, the Chinese liver fluke.
Expressed sequence tag (EST) pools represent partial profiles of the gene expressions of organisms. In an effort to construct a Clonorchis sinensis EST pool, 2,387 ESTs were collected from an adult C. sinensis cDNA library and assembled into 1,573 clusters. Of these clusters, 1,225 ESTs (51%) were singletons and 348 clusters consisted of more than two ESTs. There were 848 clusters (54%) that shared significant identity with previously reported proteins, and of these, 401 clusters were categorized into 11 major functional protein classes. Three cDNA clones of fructose-1,6-bisphosphate (FBP) aldolase were selected from the C. sinensis EST pool and analyzed for phylogenic clustering. FBP clones encoded a complete polypeptide, which shared significant identity to those of vertebrate and invertebrate animals and clustered with those of trematodes. We believe that the EST pool described can be confidently used as a platform in multigene researches on C. sinensis gene expression.
Cho
Pyo Yun
PY
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul 156-756, South Korea.
Lee
Mi Jung
MJ
Kim
Tae Im
TI
Kang
Shin-Yong
SY
Hong
Sung-Jong
SJ
D000595:Amino Acid Sequence; D000818:Animals; D003003:Clonorchiasis / Q000469:parasitology*; D003004:Clonorchis sinensis / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D018076:DNA, Complementary; D018509:DNA, Helminth; D020224:Expressed Sequence Tags; D005399:Fishes / Q000469:parasitology; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry / Q000235:genetics*; D015723:Gene Library; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D010802:Phylogeny; D011817:Rabbits; D016415:Sequence Alignment
D018076:DNA, Complementary; D018509:DNA, Helminth; D007527:Isoenzymes; D005634:Fructose-Bisphosphate Aldolase