Applications of microscopy to genetic therapy of cystic fibrosis and other human diseases.
Gene therapy has become an extremely important and active field of biomedical research. Microscopy is an integral component of this effort. This chapter presents an overview of imaging techniques used in our facility in support of cystic fibrosis gene therapy research. Instrumentation used in these studies includes light and confocal microscopy, transmission electron microscopy, and scanning electron microscopy. Techniques outlined include negative staining, cryo-electron microscopy, three-dimentional reconstruction, enzyme cytochemistry, immunocytochemistry, and fluorescence imaging.
Moninger
Thomas O
TO
Central Microscopy Research Facility, Department of Internal Medicine, University of Iowa, Iowa City, IA, USA.
Nessler
Randy A
RA
Moore
Kenneth C
KC
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D018441:Biofilms; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000235:genetics* / Q000382:microbiology / Q000628:therapy*; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D020545:Respiratory Mucosa / Q000166:cytology