Application of comparative genomics in developing molecular markers tightly linked to the virus resistance gene Rsv4 in soybean.
The Rsv4 gene confers resistance to all the known strain groups of soybean mosaic virus in soybean (Glycine max (L.) Merr.). To construct a fine genetic map near Rsv4 in soybean, we employed a comparative genomics approach that used genome sequence information of the model legume Lotus japonicus. Sequences of the soybean expressed sequence tags (ESTs) AI856415 and BF070293 mapping to one side of the Rsv4 gene showed high similarity with gene sequences of the transformation-competent artificial chromosome (TAC) clone LjT32P24 of Lotus. LjT32P24 is tightly linked to another sequenced TAC clone, LjT26I01, in Lotus. A new marker, AW307114A, developed from soybean EST AW307114, which is homologous to a Lotus gene within LjT26I01, was mapped to the other side of the Rsv4 gene. The identification of the microsyntenic relationship facilitated the development of additional 2 EST markers between BF070293-S and AW307114A bracketing the Rsv4 gene. Several other markers developed in this study were mapped to putative homoeologous or duplicated chromosomal regions in soybean. Alignment between the soybean maps indicated that Rsv4 is located near a local chromosomal rearrangement. This targeted comparative mapping serves to provide a foundation for marker-assisted selection and cloning of the Rsv4 gene.
Hwang
Tae-Young
TY
BioEvaluation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
Moon
Jung-Kyung
JK
Yu
Seok
S
Yang
Kiwoung
K
Mohankumar
Subbarayalu
S
Yu
Yong Hwan
YH
Lee
Yeong Ho
YH
Kim
Hong Sig
HS
Kim
Hwan Mook
HM
Maroof
M A Saghai
MA
Jeong
Soon-Chun
SC
D002874:Chromosome Mapping; D008040:Genetic Linkage; D005819:Genetic Markers; D023281:Genomics / Q000379:methods*; D007113:Immunity, Innate / Q000235:genetics*; D000070116:Lotus / Q000235:genetics*; D009029:Mosaic Viruses / Q000472:pathogenicity; D017421:Sequence Analysis; D013025:Glycine max / Q000235:genetics* / Q000821:virology; D026801:Synteny
D005819:Genetic Markers