title: Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii lambda-carrageenan using carrabiose oligosaccharides prepared by enzymatic hydrolysis.
abstract: lambda-Carrageenan extracted from Gigartina skottsbergii tetrasporophyte was completely digested by a purified Pseudoalteromonas carrageenovora lambda-carrageenase. The main digestion products were fractionated and analysed by (1)H and (13)C NMR spectroscopy. All the oligosaccharides observed belong to the neo-carrabiose oligosaccharide series indicating that the lambda-carrageenase cleaves the beta-(1-->4) glycosidic bonds. (1)H and (13)C NMR spectra recorded on oligomers from DP 2 to DP 8 were fully interpreted allowing unambiguous assignment of the lambda-carrageenan spectra. Besides the typical oligo-lambda-carrageenans, we have also characterised a heptasulfated tetrasaccharide which demonstrates the random over-sulfation along the chain of G. skottsbergii lambda-carrageenan.
authors.affiliation: Végétaux Marins et Biomolécules, UMR 7139 (CNRS/UPMC), Station Biologique, Roscoff, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Guibet
authors.forename: Nelly
authors.initials: N
authors.lastname: Kervarec
authors.forename: Sabine
authors.initials: S
authors.lastname: Génicot
authors.forename: Yann
authors.initials: Y
authors.lastname: Chevolot
authors.forename: William
authors.initials: W
authors.lastname: Helbert
mesh_terms: D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002351:Carrageenan / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D005685:Galactans / Q000032:analysis / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D031161:Pseudoalteromonas / Q000201:enzymology; D000461:Rhodophyta / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D005685:Galactans; D009844:Oligosaccharides; C080061:carrabiose; D002351:Carrageenan; D006026:Glycoside Hydrolases