[Analysis of the volatile components of YL2000 decoction by GC-MS].
OBJECTIVE
To analyze the volatile components of YL2000 decoction, which contain four herbs: rhizome and root of the Notopterygium incisum, the root of the Angelica pubescens f. biserrata, Scutellaria baicalensis and Coptis chinensis and investigate the changes of volatile constituents from those four herbs before and after compatibility of the herbal medicines.


METHODS
The volatile components of YL2000 decoction were extracted by water-steam distillation, separated and identified by GC-MS. The relative percent content of each volatile component was quantified by area normalization method. The volatile components of YL2000 decoction were compared with the composition of the volatile oil from individual herb respectively.


RESULTS
39 of the 146 separated constituents in volatile oil of YL2000 decoction, accounting for 85.66%, were identified and quantified. After compatibility of the herbal medicines, most volatile oil components reported by high proportion in individual herb were not detected, in the mean time, some components in volatile oil of YL2000 decoction have never been reported before in those of all four herbs.


CONCLUSIONS
The changes of volatile oil from those four herbs before and after compatibility of the herbal medicines indicate that solubilization, chemical reactions and evaporation of some volatile components during decocting may induce changes of several components.
Zhang
Xiao-Na
XN
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Chen
Long
L
Xing
Dong-Ming
DM
Du
Li-Jun
LJ
Ding
Yi
Y
Wang
Wei
W
D029969:Angelica / Q000737:chemistry; D019661:Apiaceae / Q000737:chemistry; D039323:Coptis / Q000737:chemistry; D003374:Coumarins / Q000032:analysis; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D006838:Hydrocarbons / Q000032:analysis; D009822:Oils, Volatile / Q000032:analysis* / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D031364:Scutellaria baicalensis / Q000737:chemistry
D003374:Coumarins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; D006838:Hydrocarbons; D009822:Oils, Volatile