Effect of six decades of selective breeding on soybean protein composition and quality: a biochemical and molecular analysis.
To evaluate the extent of the genetic change and its effects on the seed protein composition of soybean cultivars released during the past 60 years, representative ancestral cultivars and those derived from selective breeding were grown in a side-by-side comparison. Total seed protein content, determined by combustion analysis of nitrogen, revealed a decline in the protein content after decades of selection and breeding. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis comparison of protein profiles of the soybean cultivars indicated that relative expression of most of the seed storage proteins had not varied substantially from the ancestral lines to the present commercial cultivars. There was noticeably less beta-subunit of beta-conglycinin, a protein devoid of sulfur amino acids, in the modern cultivars represented by Mustang, Pioneer 93B09, and Asgrow 3602. Comparison of the amino acid profiles of soybean seed, a benchmark of the protein's nutritional quality, revealed that the ancestral progenitor, G. soja, was significantly higher in cysteine, glutamic acid, histidine, and arginine than either the ancestral or the modern cultivars. Selective breeding over the past 60 years minimally affected the overall amino acid composition. The degree of divergence in the DNA sequence of the genes encoding glycinin and beta-conglycinin in the ancestral and modern cultivars was investigated using Southern hybridization and the polymerase chain reaction. Even though some restriction fragment polymorphisms could be detected, overall, the banding patterns were remarkably similar among the ancestral cultivars and those derived from them, suggesting a high degree of conservation of seed-storage protein genes. The results of our study suggest that selection and breeding for yield during the past 60 years had no major influence on the protein composition, ostensibly because of limited genetic diversity among the parental lines.
Mahmoud
Ahmed A
AA
Plant Science Division and Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, Maryland 20705, USA.
Natarajan
Savithiry S
SS
Bennett
John O
JO
Mawhinney
Thomas P
TP
Wiebold
William J
WJ
Krishnan
Hari B
HB
D000596:Amino Acids / Q000032:analysis; D052179:Antigens, Plant; D001947:Breeding; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D005916:Globulins / Q000032:analysis; D009753:Nutritive Value; D055314:Seed Storage Proteins; D012639:Seeds / Q000737:chemistry*; D030262:Soybean Proteins / Q000032:analysis* / Q000235:genetics; D013025:Glycine max / Q000737:chemistry / Q000235:genetics*
D000596:Amino Acids; D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max