Visualizing the cortical representation of whisker touch: voltage-sensitive dye imaging in freely moving mice.
Voltage-sensitive dye imaging resolves the spatiotemporal dynamics of supragranular subthreshold cortical activity with millisecond temporal resolution and subcolumnar spatial resolution. We used a flexible fiber optic image bundle to visualize voltage-sensitive dye dynamics in the barrel cortex of freely moving mice while simultaneously filming whisker-related behavior to generate two movies matched frame-by-frame with a temporal resolution of up to 2 ms. Sensory responses evoked by passive whisker stimulation lasted longer and spread further across the barrel cortex in awake mice compared to anesthetized mice. Passively evoked sensory responses were large during behaviorally quiet periods and small during active whisking. However, as an exploring mouse approached an object while whisking, large-amplitude, propagating cortical sensory activity was evoked by active whisker-touch. These experiments demonstrate that fiber optics can be used to image cortical sensory activity with high resolution in freely moving animals. The results demonstrate differential processing of sensory input depending upon behavior.
Ferezou
Isabelle
I
Laboratory of Sensory Processing, Brain Mind Institute, SV-BMI-LSENS AAB 105, Station 15, Ecole Polytechnique Federale de Lausanne, CH-1015 Lausanne, Switzerland.
Bolea
Sonia
S
Petersen
Carl C H
CC
D000768:Anesthesia, General; D000818:Animals; D001522:Behavior, Animal; D001931:Brain Mapping; D003952:Diagnostic Imaging; D005073:Evoked Potentials, Somatosensory / Q000502:physiology; D005336:Fiber Optic Technology / Q000379:methods*; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D051379:Mice; D055100:Optical Fibers; D018408:Patch-Clamp Techniques; D013003:Somatosensory Cortex / Q000502:physiology*; D014738:Vibrissae / Q000294:innervation*; D014851:Wakefulness / Q000502:physiology
D005456:Fluorescent Dyes