Effect of two insecticides and two herbicides on the porcine sperm motility patterns using computer-assisted semen analysis (CASA) in vitro.
The recent decline in sperm concentration observed in men has developed over a short period of time, suggesting that it could be the result of environmental factors. The present study has evaluated the effects of insecticides Malathion and Diazinon, and herbicides Atrazine and Fenoxaprop-Ethyl on porcine sperm viability and motility patterns in vitro using the eosin-nigrosin staining and a computer-assisted semen analyzer (CASA), respectively. Malathion and Fenoxaprop-Ethyl exerted more deleterious effects than Diazinon and Atrazine. Progressive sperm motility was strongly affected whereas the effect on sperm viability was less pronounced. This suggests that a reduction of sperm motility is not necessarily the result of sperm death. Since sperm motility is dependent on energy metabolism the mechanism of action of these pesticides might be mediated at the level of the mitochondrion, producing a delay in motility and eventual cell death.
Betancourt
Miguel
M
Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco 186, CP 09340, DF, México. bet@xanum.uam.mx
Reséndiz
Alicia
A
Fierro
Eduardo Casas Y Reyna
EC
D000818:Animals; D001280:Atrazine / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D003976:Diazinon / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D006540:Herbicides / Q000633:toxicity*; D007090:Image Interpretation, Computer-Assisted; D066298:In Vitro Techniques; D007306:Insecticides / Q000633:toxicity*; D008294:Malathion / Q000633:toxicity; D008297:Male; D010080:Oxazoles / Q000633:toxicity; D011422:Propionates / Q000633:toxicity; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects; D013552:Swine
D006540:Herbicides; D007306:Insecticides; D010080:Oxazoles; D011422:Propionates; C416661:fenoxaprop ethyl; D001280:Atrazine; D008294:Malathion; D003976:Diazinon