title: Identification of enzyme responsible for erythritol utilization and reaction product in yeast Lipomyces starkeyi.
abstract: We have identified the enzyme responsible for erythritol utilization and its reaction product in the yeast Lipomyces starkeyi CBS 1807. The enzyme, a polyol dehydrogenase requiring NAD+ as a coenzyme, was induced by erythritol in this yeast. We confirmed that the enzyme product was L-erythrulose by MS, NMR, and polarimeter analyses, meaning that we clarified the first step of erythritol utilization in yeasts for the first time. In the case of the oxidative reaction, D-threitol, (2R,3R)-2,3-butanediol, and erythritol were much better substrates than 21 other polyols tested. These three substrates are tetroses and have an R configuration at C-3, and whose third carbon results in easiest oxidation in this enzyme. The research of the substrate specificity in the reductive reaction demonstrated that L-erythrulose and dihydroxyacetone were better substrates, that D-acetoin was inactive and L-erythrose (aldose) was slightly active.
authors.affiliation: Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, 1-8-14 Kanda-surugadai, Chiyoda-ku, Tokyo 101-8308, Japan. nisimura@chem.cst.nihon-u.ac.jp
authors.forename: Katsushi
authors.initials: K
authors.lastname: Nishimura
authors.forename: Teiko
authors.initials: T
authors.lastname: Harada
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Arita
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Watanabe
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Iwabuki
authors.forename: Asako
authors.initials: A
authors.lastname: Terada
authors.forename: Takafumi
authors.initials: T
authors.lastname: Naganuma
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Uzuka
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry; D002474:Cell-Free System; D004098:Dihydroxyacetone / Q000737:chemistry; D004896:Erythritol / Q000378:metabolism*; D007064:L-Iditol 2-Dehydrogenase / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity
chemical_list: D002241:Carbohydrates; D007064:L-Iditol 2-Dehydrogenase; D004098:Dihydroxyacetone; D004896:Erythritol