Reduction of allergenic proteins by the effect of the ripening inhibitor (rin) mutant gene in an F1 hybrid of the rin mutant tomato.
The ripening inhibitor (rin) mutant tomato yields non-ripening fruit, and the rin hybrid fruit (RIN/rin) shows an intermediate phenotype between the wild and mutant fruit, that is, red-ripe and extended shelf life. We found by a microarray analysis that the genes encoding possible allergenic proteins were expressed at a significantly lower level in the rin hybrid fruit than in the wild-type fruit. These allergenic proteins, which were beta-fructofuranosidase and polygalacturonase 2A (PG-2A), were confirmed to accumulate at a lower level in the rin hybrid fruit than in the wild-type fruit. The immunoglobulin E (IgE) in serum from a tomato-allergic patient showed lower reactivity to the extract of the rin hybrid fruit than to that of the wild fruit. These results suggest that the rin gene has the potential to regulate allergen accumulation in tomato fruit.
Kitagawa
Mamiko
M
Research Institute, Kagome Co., Ltd. Tochigi, Japan.
Moriyama
Tatsuya
T
Ito
Hirotaka
H
Ozasa
Sayaka
S
Adachi
Atsuko
A
Yasuda
Junichi
J
Ookura
Tetsuya
T
Inakuma
Takahiro
T
Kasumi
Takafumi
T
Ishiguro
Yukio
Y
Ito
Yasuhiro
Y
D052179:Antigens, Plant / Q000235:genetics* / Q000276:immunology*; D002678:Chimera / Q000235:genetics; D005512:Food Hypersensitivity / Q000276:immunology*; D015870:Gene Expression; D017343:Genes, Plant; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D018551:Solanum lycopersicum / Q000009:adverse effects* / Q000235:genetics / Q000276:immunology; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology*; D011096:Polygalacturonase / Q000235:genetics / Q000276:immunology; D043324:beta-Fructofuranosidase / Q000235:genetics / Q000276:immunology
D052179:Antigens, Plant; D010940:Plant Proteins; D007073:Immunoglobulin E; D011096:Polygalacturonase; D043324:beta-Fructofuranosidase