Use of near infrared emission spectroscopy in the study of supporting materials and stationary phases for liquid chromatography.
Near infrared emission spectroscopy (NIRES) has been investigated in the study of different materials employed in liquid chromatography. The samples were heated in a nitrogen atmosphere and the emission spectra were obtained using a lab-made NIRES instrument. Through principal component analysis (PCA) using the raw emission spectra, it was possible to distinguish different materials according to their physical and/or chemical characteristics. Linear relationships between emissivity spectra and the contents of the coating material or the specific surface areas was observed for stationary phases or bare silicas, respectively. Furthermore, the thermal stability of stationary phases could be followed in real time.
Gonzaga
Fabiano Barbieri
FB
Instituto de Química, Universidade Estadual de Campinas, SP, Brazil; Caixa Postal 6154, CEP 13084-971, Campinas, SP, Brazil.
Jardim
Isabel C S F
IC
Pasquini
Celio
C
D000465:Algorithms; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D025341:Principal Component Analysis / Q000379:methods; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000032:analysis / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013696:Temperature; D013816:Thermodynamics
D012822:Silicon Dioxide