AFLP fingerprinting for the analysis of genetic diversity within Necator americanus.
In the present study, we utilised the method of AFLP to screen for genetic variation within and among individuals of the blood-feeding human hookworm Necator americanus (Nematoda) from Africa, Asia and South America. A total of 45 adult worms (i.e. 20 from Ghana, 16 from Colombia and 9 from Nepal) were subjected to analysis using the restriction enzyme/primer combination HindIII+AG/BglII+AC. Cluster analysis divided N. americanus into multiple, genetically distinct groups, consistent with previous findings using ribosomal and mitochondrial DNA data sets. The results demonstrated the usefulness of AFLP fingerprinting for establishing genetic variation within N. americanus and reinforce its applicability to other parasitic helminths of human and/or veterinary health importance.
de Gruijter
Johanna M
JM
Leiden University Medical Center, University of Leiden, PO Box 9600, 2300 RC Leiden, The Netherlands. j.degruijter@erasmusmc.nl
Polderman
Anton M
AM
Dijkshoorn
Lenie
L
Roberts
Helen
H
Ziem
Juventus
J
Kunwar
Chhatra B
CB
Gasser
Robin B
RB
D000818:Animals; D016172:DNA Fingerprinting; D018509:DNA, Helminth / Q000032:analysis*; D014644:Genetic Variation; D006801:Humans; D017221:Necator americanus / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny
D018509:DNA, Helminth