title: Relative importance of CD4+ and CD8+ T cells in the resolution of Chlamydophila abortus primary infection in mice.
abstract: The role of the specific cellular immune response is well established in Chlamydiaceae infections, but the importance of each T-cell subset seems to be species-dependent. This study was designed to clarify the role of T-cell subsets in the response to Chlamydophila abortus primary infection. C57BL/6 mice were depleted of CD4+ or CD8+, or both, by monoclonal antibody injections and subsequently infected with C. abortus. Mice were killed at intervals and samples were collected for bacteriological and histopathological analysis. Also carried out were spleen cell culture, cytokine quantification, immunolabelling for C. abortus antigen, and a TUNEL assay for apoptosis. CD8+ T cell-depleted mice all died within 12 days of C. abortus infection, while no mortality was observed in the other groups; surprisingly, CD4+ T cell-depleted mice showed lower morbidity (expressed as weight loss) than did a non-depleted (control) group. CD8+ T cell-depleted mice also differed from the other groups in showing a significantly higher chlamydial burden in the liver. CD8+ T cell-depleted mice also had a higher number of apoptotic cells in hepatic inflammatory foci and showed exacerbated IFN-gamma production by spleen cells after specific stimulation. Simultaneous depletion of both T-cell subpopulations led to a chronic infection, but not to early mortality. It is concluded that CD8+ T cells may play a role in the regulatory control of the CD4+ T-cell response and may have a direct cytotoxic or IFN-gamma-mediated effect on infected cells.
authors.affiliation: Departamento de Histología y Anatomía Patológica, Universidad de Murcia, Campus de Espinardo, Spain.
authors.forename: C M
authors.initials: CM
authors.lastname: Martínez
authors.forename: A J
authors.initials: AJ
authors.lastname: Buendía
authors.forename: J
authors.initials: J
authors.lastname: Sánchez
authors.forename: N
authors.initials: N
authors.lastname: Ortega
authors.forename: M R
authors.initials: MR
authors.lastname: Caro
authors.forename: M C
authors.initials: MC
authors.lastname: Gallego
authors.forename: J A
authors.initials: JA
authors.lastname: Navarro
authors.forename: F
authors.initials: F
authors.lastname: Cuello
authors.forename: J
authors.initials: J
authors.lastname: Salinas
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002452:Cell Count; D002478:Cells, Cultured; D023501:Chlamydophila / Q000276:immunology*; D023521:Chlamydophila Infections / Q000276:immunology* / Q000473:pathology / Q000635:transmission; D004195:Disease Models, Animal; D005260:Female; D020287:In Situ Nick-End Labeling; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008212:Lymphocyte Depletion; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D015847:Interleukin-4; D007371:Interferon-gamma