title: [Expression and location of UBAP1 protein associated with nasopharyngeal carcinoma].
abstract: OBJECTIVE
  To analyze the expression and location of coding protein of UBAP1 gene and to understand the relationship between the expression pattern of the protein and cell carcinogenesis.
  
  
  METHODS
  Bioinformatics was used to analyze the protein character to provide an available clue of subsequent research. The codon frame cDNA was amplified by PCR, and subcloned into enhance green fluorescence protein (EGFP) of pEGFP-C2. The recombinant plasmid was transfected into HNE1 cells. The expression of coding protein was observed by fluorescence microscopy.
  
  
  RESULTS
  The expressed GFP-fusion protein generated striking green fluorescence in the cytoplasm in HNE1 cells. EGFP/UBAP1 was expressed and existed mainly in the nuclear, especially accumulated on the nuclear envelope.
  
  
  CONCLUSIONS
  The expression difference in HNE1 might be related to the carcinogenesis of NPC.
authors.affiliation: Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Zhao-yang
authors.initials: ZY
authors.lastname: Zeng
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Zhou
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Zhang
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Tang
authors.forename: Wei-fang
authors.initials: WF
authors.lastname: Li
authors.forename: Gui-yuan
authors.initials: GY
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D002352:Carrier Proteins; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; C469867:UBAP1 protein, human; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins