Synthesis and structure analysis of novel disaccharides containing D-psicose produced by endo-1,4-beta-D-xylanase from Aspergillus sojae.
Xylosylpsicoses (XPs) were synthesized by transglycosylation (TG) with arabinoxylan (AX) and D-psicose (D-psi) using endo-1,4-beta-D-xylanase (EC 3.2.1.8) from Aspergillus sojae. The structures of XPs were analyzed by HPLC to determine sugar composition and molecular mass, by methylation analysis using GC-MS to determine linkage, and by 1H- and 13C-NMR spectrometries to obtain the anomeric configuration of glycosidic linkage. By chemical analysis, it was found that the structures of XPs are Xylp beta1-4Psip, Xylp beta1-5Psip, Xylp beta1-1Psif and Xylp beta1-6Psif.
Oshima
Hisaka
H
Food Research Institute of Kagawa Prefectural Industrial Technology Center, 587-1 Goutou-cho, Takamatsu-shi, Kagawa 761-8031, Japan.
Kimura
Isao
I
Izumori
Ken
K
D001230:Aspergillus / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000737:chemistry*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000378:metabolism; D005632:Fructose / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011108:Polymers / Q000737:chemistry; D013053:Spectrophotometry; D013997:Time Factors
D002241:Carbohydrates; D004187:Disaccharides; D011108:Polymers; C003243:psicose; D005632:Fructose; D043364:Endo-1,4-beta Xylanases