Proteomics technology and therapeutics.
1. Proteins are the workhorses of biological systems and, thus, it is not surprising that the field of proteomics has flourished in recent years. The present review briefly surveys the general proteomic approaches taken to identify drug targets and to evaluate drug efficacy and toxicity in the preclinical and clinical settings. These generally fall into three basic categories: a profiling approach, a functional approach and a structural approach. 2. The current 'tools' used and applied to the drug-discovery process will be highlighted. These include the two-dimensional gel electrophoresis, liquid chromatography, mass spectrometry, isotope-coded affinity tag and protein biochips. 3. Clearly, proteomics has begun to set a foothold in every stage of the drug-discovery process. Future developments in this area will likely make a significant impact in our quest for better, safer and cost-effective drugs.
Hong
Michelle Li Wen
ML
Functional Genomic Laboratories, Department of Biological Sciences, National University of Singapore, Singapore.
Jiang
Nan
N
Gopinath
Sravanthy
S
Chew
Fook Tim
FT
D000818:Animals; D002853:Chromatography, Liquid; D015195:Drug Design; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007553:Isotope Labeling; D013058:Mass Spectrometry; D040081:Protein Array Analysis; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods / Q000639:trends
D011506:Proteins