Isolation and characterization of the canine NKX2-5 gene.
We report the first isolation and characterization of the canine NKX2-5 gene. This canine homologue has high homology in genomic structure and functional domains to other NKX2-5 across a number of different species. Given the critical role of NKX2-5 in cardiac morphogenesis as seen in human and mouse studies of congenital heart defects, the availability of the canine NKX2-5 provides a good starting point for identifying mutations that may be responsible for certain forms of canine congenital heart defects.
Hyun
C
C
Section of Internal Medicine, School of Veterinary Medicine, College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea. hyun5188@kangwon.ac.kr
Lavulo
L
L
O'Leary
C
C
Kim
J-T
JT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004285:Dogs / Q000235:genetics*; D005260:Female; D023061:Gene Order; D005801:Genes, Homeobox / Q000235:genetics*; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins; C095552:NKX2-5 protein, human; D014157:Transcription Factors