title: Observation of all the components of elastic constants using tetragonal hen egg-white lysozyme crystals dehydrated at 42% relative humidity.
abstract: Success in measuring transverse sound velocity allowed us to determine, for the first time, all six elastic constants of a protein crystal. An ultrasonic pulse-echo method was used to perform sound velocity measurements on tetragonal hen egg-white (HEW) lysozyme crystals that were partially dehydrated at 42% relative humidity. The measurements were performed using the (110), (101), and (001) crystallographic faces. Thus, all six elastic constants of the dehydrated tetragonal HEW lysozyme crystals were determined: C11=C22=12.44 GPa, C12=7.03 GPa, C13=C23=8.36 GPa, C33=12.79 GPa, C44=C55=2.97 GPa, and C66=2.63 GPa. In addition, for the hydrated crystals, the longitudinal sound velocities along the [110] direction and the direction normal to the (101) face were measured. From these results, all the components of elastic constants in the hydrated crystals were extrapolated.
authors.affiliation: Graduate School of Integrated Science, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Koizumi
authors.forename: M
authors.initials: M
authors.lastname: Tachibana
authors.forename: K
authors.initials: K
authors.lastname: Kojima
mesh_terms: D000818:Animals; D002645:Chickens; D003198:Computer Simulation; D003460:Crystallization / Q000379:methods*; D003461:Crystallography / Q000379:methods*; D003890:Desiccation; D004548:Elasticity; D008956:Models, Chemical; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000032:analysis / Q000737:chemistry; D009113:Muramidase / Q000032:analysis* / Q000737:chemistry*; D011487:Protein Conformation; D013314:Stress, Mechanical; D014867:Water / Q000737:chemistry*
chemical_list: D046912:Multiprotein Complexes; D014867:Water; C421864:hen egg lysozyme; D009113:Muramidase