title: Integrin alpha 11 is a novel type I interferon stimulated gene.
abstract: Interferons (IFNs) are a family of cytokines that have many biological functions in the cell, including regulation of cellular growth, differentiation, immunomodulation, and viral replication by inducing a set of interferon stimulated genes (ISGs). Based on their structure and biological activities IFNs are subdivided into two groups: type I IFNs, which includes IFN-alpha and IFN-beta and type II IFNs, represented by IFN-gamma. The aim of this work was to investigate whether integrin alpha 11 (ITGA-11), a novel collagen-binding integrin, is responsive to type I IFN treatment. Our findings indicated that type I IFNs were able to induce the ITGA-11 mRNA levels in T98G cells. Increased levels of ITGA-11 protein were also observed in IFN-treated cells. The in vivo induction of ITGA-11 was detected in spleen and lungs of IFN-treated BALB/c mice. T98G cells infected with Murine encephalomyocarditis virus showed increased levels of ITGA-11 mRNA and protein. We observed that the ITGA-11 promoter has binding sites for transcriptional factors regulated by IFNs and the double-stranded RNA dependent protein kinase (PKR). Therefore we investigated the role of PKR in the induction of ITGA-11 by using a PKR deficient mouse embryo fibroblast cell line (MEFs). PKR(-/-) MEFs treated with IFN did not show increased levels of ITGA-11 protein nor mRNA although that could be promptly detected in wild type MEFs. Taken together our data suggest that ITGA-11 is a new interferon stimulated gene.
authors.affiliation: Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Luiz Felipe
authors.initials: LF
authors.lastname: Leomil Coelho
authors.forename: Bruno Eduardo Fernandes
authors.initials: BE
authors.lastname: Mota
authors.forename: Paula Cristiane Motta
authors.initials: PC
authors.lastname: Sales
authors.forename: João Trindade
authors.initials: JT
authors.lastname: Marques
authors.forename: Jaquelline Germano
authors.initials: JG
authors.lastname: de Oliveira
authors.forename: Cláudio Antonio
authors.initials: CA
authors.lastname: Bonjardim
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Peregrino Ferreira
authors.forename: Erna Geessien
authors.initials: EG
authors.lastname: Kroon
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D039001:Integrin alpha Chains / Q000096:biosynthesis / Q000235:genetics*; D007370:Interferon Type I / Q000494:pharmacology*; D016898:Interferon-alpha / Q000502:physiology*; D016899:Interferon-beta / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D011994:Recombinant Proteins; D019892:eIF-2 Kinase / Q000172:deficiency / Q000235:genetics
chemical_list: C121535:ITGA11 protein, human; D039001:Integrin alpha Chains; D007370:Interferon Type I; D016898:Interferon-alpha; D011994:Recombinant Proteins; D016899:Interferon-beta; D019892:eIF-2 Kinase