title: Influence of pseudorabies virus proteins on neuroinvasion and neurovirulence in mice.
abstract: Neurotropism is a distinctive feature of members of the Alphaherpesvirinae. However, its molecular basis remains enigmatic. In the past, research has been focused mainly on the role of viral envelope proteins in modulating herpesvirus neuroinvasion and neurovirulence (T. C. Mettenleiter, Virus Res. 92:192-206, 2003). To further analyze the molecular requirements for neuroinvasion of the alphaherpesvirus pseudorabies virus (PrV), adult mice were infected intranasally with a set of single- or multiple-deletion mutants lacking the UL3, UL4, UL7, UL11, UL13, UL16, UL17, UL21, UL31, UL34, UL37, UL41, UL43, UL46, UL47, UL48, UL51, US3, US9, glycoprotein E (gE), gM, UL11/US9, UL11/UL16, UL16/UL21, UL11/UL16/UL21, UL11/gE, UL11/gM, UL43/gK, UL43/gM, or UL43/gK/gM genes. Neurovirulence was evaluated by measuring mean survival times compared to that after wild-type virus infection. Furthermore, by immunohistochemical detection of infected neurons, the kinetics of viral spread in the murine central nervous system was investigated.
authors.affiliation: Institute of Molecular Biology, Friedrich-Loeffler-Institut, Boddenblick 5A, D-17493 Greifswald-Insel Riems, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Klopfleisch
authors.forename: Barbara G
authors.initials: BG
authors.lastname: Klupp
authors.forename: Walter
authors.initials: W
authors.lastname: Fuchs
authors.forename: Martina
authors.initials: M
authors.lastname: Kopp
authors.forename: Jens P
authors.initials: JP
authors.lastname: Teifke
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Mettenleiter
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D020805:Central Nervous System Viral Diseases / Q000821:virology; D011558:Herpesvirus 1, Suid / Q000737:chemistry*; D007700:Kinetics; D051379:Mice; D009474:Neurons / Q000821:virology*; D011557:Pseudorabies / Q000503:physiopathology* / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000502:physiology*; D014774:Virulence
chemical_list: D000956:Antigens, Viral; D014759:Viral Envelope Proteins