title: Bacteriological and electron microscopic examination of primary intrahepatic stones.
abstract: BACKGROUND
  Primary intrahepatic cholelithiasis is usually combined with biliary tract infection. This research was undertaken to investigate the relationship between intrahepatic stones and biliary tract infection.
  
  
  METHODS
  Thirty-five bile samples and 30 stones specimens were cultured for bacteria and 12 stones specimens were examined with a scan electron microscope (SEM) or a transmission electron microscope (TEM).
  
  
  RESULTS
  94.2% bile samples and 96.7% stones specimens were positive in bacteria culture. Bacteria were found in stones under SEM and TEM.
  
  
  CONCLUSIONS
  Bacteria in stones are associated with the infection of the biliary tract.
authors.affiliation: Department of General Surgery, Second Affiliated Hospital (Shengjing Hospital), China Medical University, Shenyang 110004, China. wushuodong@hotmail.com
authors.forename: Shuo-Dong
authors.initials: SD
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.forename: Ji-Mei
authors.initials: JM
authors.lastname: Sun
mesh_terms: D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology; D001660:Biliary Tract Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000601:surgery; D005260:Female; D042882:Gallstones / Q000737:chemistry* / Q000453:epidemiology / Q000382:microbiology* / Q000648:ultrastructure; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000302:isolation & purification; D006801:Humans; D015994:Incidence; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index