[Diffusional attenuation of proton echo and control of changes in the cytoskeleton hydration level].
Using the pulsed NMR-method with pulse gradient of the magnetic field, a study was made of changes in diffusional attenuation (DA) of proton echo in germs of triticum grains under the influence of agents depolymerizing cytoskeleton elements or inhibiting their ATP-activity. At a short diffusion time, we observed respective alterations of NMR-population in a fraction of cell water molecules contributing to DA at large values of pulse gradient. It is shown that the presence in DA of a site with essentially smaller coefficient of self-diffusion, as compared with basic cell water fraction, is not a consequence of restricted diffusion phenomenon. The observed changes in NMR-population of the appropriate fraction of cell water molecules are interpreted as changes in the level of cytoskeleton hydratation occurring under the influence of the used agents. A hypothesis is proposed for the functional role of similar changes in the level of hydratation of cytoskeleton elements located inside plamodesmata in the mechanism of plasmodesmatal water permeability regulation.
Velikanov
G A
GA
Belova
L P
LP
Gaponenko
E M
EM
D003599:Cytoskeleton / Q000737:chemistry*; D004058:Diffusion; D009682:Magnetic Resonance Spectroscopy; D011522:Protons; D014908:Triticum / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D011522:Protons; D014867:Water