title: Effects of IL-1 beta on RT1-A/RT1-DM at the maternal-fetal interface during pregnancy in rats.
abstract: Interleukin-1 (IL-1beta) beta and major histocompatibility complex (MHC) play an important role during pregnancy. Expression of non-classical class MHC II RT1-DM antigen and classical class MHC I RT1-A antigen induced by IL-1beta was examined by Northern blotting, Western blotting and immunohistochemistry. IL-1beta treatment significantly increased the expression of RT1-A and RT1-DM in early and mid pregnancy. In late pregnancy, expression of RT1-DM significantly increased in uteri and decreased in placenta. Immunohistochemical studies indicated that, in early pregnancy, RT1-DM protein mainly localized to luminal and glandular uterine epithelium, and RT1-A was present in deciduas basalis, outer layer of luminal epithelium and glandular epithelium. During mid and late pregnancy, RT1-DM was present in maternal blood vessels and syncytiotrophoblast of labyrinthine zone, and RT1-A was present in maternal blood vessels and trophoblastic epithelium of the labyrinthine layers. These findings show that exogenous IL-1beta affects expression of RT1-DM and RT1-A and does not affect the localization of corresponding molecules during pregnancy.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080, China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Xia
authors.forename: Jing-Pian
authors.initials: JP
authors.lastname: Peng
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Liu
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006649:Histocompatibility Antigens / Q000096:biosynthesis*; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000502:physiology*; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000378:metabolism
chemical_list: D006649:Histocompatibility Antigens; D007375:Interleukin-1; C038353:histocompatibility antigens RT, rat; D012313:RNA