Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker.
[reaction: see text] We report the development of a safety-catch photolabile linker that allows the light-directed synthesis and spatially selective photorelease of oligonucleotides from microarrays. The linker remains stable to light during DNA synthesis, and is activated for photorelease after acidic hydrolysis. We demonstrate that the photoreleased oligonucleotides can be amplified by PCR to produce double stranded DNA. The advantages offered by this linker could aid the development of an automated gene synthesis platform.
Flickinger
Shane T
ST
The Department of Chemistry, University of Wisconsin-Madison, 53706, USA.
Patel
Madhusudan
M
Binkowski
Brock F
BF
Lowe
Aaron M
AM
Li
Mo-Huang
MH
Kim
Changhan
C
Cerrina
Franco
F
Belshaw
Peter J
PJ
D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010782:Photolysis
D009841:Oligonucleotides; D004247:DNA