title: A tryptophan amphiphilic tetramerization domain-containing acetylcholinesterase from the bovine lungworm, Dictyocaulus viviparus.
abstract: Acetylcholine (ACh) is one of an array of neurotransmitters used by invertebrates and, analogous to vertebrate nervous systems, acetylcholinesterase (AChE) regulates synaptic levels of this transmitter. Similar to other invertebrates, nematodes possess several AChE genes. This is in contrast to vertebrates, which have a single AChE gene, transcripts of which are alternatively spliced to produce different types of the enzyme which vary at their C-termini. Parasitic nematodes have a repertoire of AChE genes which include those encoding neuromuscular AChEs and those genes which code for secreted AChEs. The latter proteins exist as soluble monomers released by the parasite during infection and these AChE are distinct from those enzymes which the nematodes use for synaptic transmission in their neuromuscular system. Thus far, Dictyocaulus viviparus is the only animal-parasitic nematode for which distinct genes that encode both neuromuscular and secreted AChEs have been defined. Here, we describe the isolation and characterization of a cDNA encoding a putative neuromuscular AChE from D. viviparus which contains a tryptophan amphiphilic tetramerization (WAT) domain at its C-terminus analogous to the common 'tailed' AChE form found in the neuromuscular systems of vertebrates and in the ACE-1 AChE from Caenorhabditis elegans. This enzyme differs from the previously isolated, D. viviparus neuromuscular AChE (Dv-ACE-2), which is a glycosylphosphatidylinositol-anchored variant analogous to vertebrate 'hydrophobic' AChE.
authors.affiliation: Division of Parasitology, Moredun Research Institute, Pentlands Science Park, Edinburgh. Jacqui.Matthews@moredun.ac.uk
authors.forename: J B
authors.initials: JB
authors.lastname: Matthews
authors.forename: O
authors.initials: O
authors.lastname: Lazari
authors.forename: A J
authors.initials: AJ
authors.lastname: Davidson
authors.forename: S
authors.initials: S
authors.lastname: Warren
authors.forename: M E
authors.initials: ME
authors.lastname: Selkirk
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D004021:Dictyocaulus / Q000201:enzymology* / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000096:biosynthesis; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D015801:Helminth Proteins / Q000737:chemistry; D007814:Larva / Q000201:enzymology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D013997:Time Factors; D014364:Tryptophan / Q000737:chemistry* / Q000235:genetics
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D014364:Tryptophan; D003576:Electron Transport Complex IV; D000110:Acetylcholinesterase