Development of luminescent Mycobacterium avium subsp. paratuberculosis for rapid screening of vaccine candidates in mice.
Mycobacterium avium subsp. paratuberculosis is a slowly growing mycobacterial species, requiring 6 to 8 weeks of culture before colonies can be counted visually. Here, we describe the development of luminescent M. avium subsp. paratuberculosis expressing luxAB genes of Vibrio harveyi and its use for vaccine testing in an experimental mouse model, replacing fastidious CFU counting by rapid luminometry.
Rosseels
Valérie
V
WIV-Pasteur Institute Brussels, 642 Engelandstraat, B-1180 Brussels, Belgium. khuygen@pasteur.be.
Roupie
Virginie
V
Zinniel
Denise
D
Barletta
Raúl G
RG
Huygen
Kris
K
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D005260:Female; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D051379:Mice; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D010957:Plasmids; D014169:Transformation, Bacterial
D001500:BCG Vaccine; D001428:Bacterial Vaccines; D008156:Luciferases