title: [Application of four ELISA techniques (two for IgM and two for IgG) for serological diagnosis of an outbreak of Q fever].
abstract: OBJECTIVE
  To evaluate four ELISAs (commercialized by Panbio and Serion) for IgM and IgG detection in an outbreak of Q fever.
  
  
  METHODS
  Fifty-three serum samples corresponding to 29 persons were processed. An indirect immunofluorescence assay was used as the reference method.
  
  
  RESULTS
  The sensitivity and specificity for IgM were 100% and 74% (Panbio), and 91% and 88% (Serion). IgG sensitivity was 72% (Panbio) and 89% (Serion). The specificity of both techniques for IgG was 97%.
  
  
  CONCLUSIONS
  Both ELISAs seem to be good IgM screening methods for use during Q fever outbreaks.
authors.affiliation: Laboratorio Regional de Salud Pública, Instituto de Salud Pública, Comunidad de Madrid, Spain. juan.sanz@salud.madrid.org
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Sanz
authors.forename: Rosa
authors.initials: R
authors.lastname: de los Ríos
authors.forename: Fernando
authors.initials: F
authors.lastname: Martín
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Tébar
authors.forename: Isabel
authors.initials: I
authors.lastname: Jado
authors.forename: Pedro
authors.initials: P
authors.lastname: Anda
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D016997:Coxiella burnetii / Q000276:immunology*; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011778:Q Fever / Q000175:diagnosis* / Q000453:epidemiology; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M