[Effect of androgen on nitric oxiode synthase activity in the kidney of rats with ischemic injury].
OBJECTIVE
To investigate the effect of androgen on nitric oxide synthase activity and kidney function in the kidney of rats with ischemic injury.


METHODS
Fourty-eight adult healthy male SD rats were randomly divided into the renal ischemia group (n = 24 ) and the orchidectomized group with renal ischemia (n = 24). The two groups were randomly divided into 4 groups by the survival time: 0, 1, 3 and 7 days. NADPH diaphorase histochemistry was used to measure the nitric oxide synthase activity of the kidney with ischemic injury in the orchidectomized group and the control group. The image analysis technology system was used to quantify the NOS. BUN and BCr in the plasma were detected when the rats were executed.


RESULTS
The NOS histochemical staining of the kidney was mainly distributed in the renal tubule, macula densa and low in the renal glomerular. The NOS activity of macula densa significantly decreased in the orchidectomized group compared with that in the control group (P < 0.05) . BUN and Bcr have no significant difference on 0, 1 and 7 days (P > 0.05) , but on the third day the data markedly increased (P < 0.05).


CONCLUSIONS
Androgen can adjust the blood flux of glomerular by regulating the NOS activity of macula densa, and affect the kidney function.
Zeng
Qing
Q
Department of Urology, Third Xiangya Hospital, Central South University, Changsha, China. zqdoctor6@yahoo.com.cn
Liu
Zheng-qing
ZQ
Jiang
Xian-zhen
XZ
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001806:Blood Urea Nitrogen; D007668:Kidney / Q000098:blood supply* / Q000503:physiopathology; D008297:Male; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D015427:Reperfusion Injury / Q000201:enzymology*
D000728:Androgens; D019001:Nitric Oxide Synthase