Development and validation of a capillary electrophoresis method with laser-induced fluorescence detection for the determination of captopril in human urine and pharmaceutical preparations.
This study describes the development of a CE method for the analysis of the antihypertensive drug captopril using LIF detection. The method is based on the derivatization of captopril with the fluorescent label 5-iodoacetamidofluorescein. The optimization of the electrophoretic electrolyte composition together with other variables, such as applied voltage and injection time, resulted in a solution of 20 mM phosphate buffer adjusted to pH 12.0. The calibration curve for the fluorescent captopril derivative was linear in the concentration range 3.5-6000 ng/mL with a detection limit of 0.5 ng/mL. Intra- and interday precision (at a concentration of about 100 times the LOD) were less than 0.86 and 1.16%, respectively, both expressed as RSD. The assay was successfully used for quantification of captopril in some marketed pharmaceutical preparations and urine samples.
Pérez-Ruiz
Tomás
T
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Murcia, Spain. tpr@um.es
Martínez-Lozano
Carmen
C
Galera
Raquel
R
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis* / Q000652:urine; D000959:Antihypertensive Agents / Q000032:analysis* / Q000652:urine; D002216:Captopril / Q000032:analysis* / Q000652:urine; D002626:Chemistry, Pharmaceutical; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007834:Lasers; D004364:Pharmaceutical Preparations / Q000032:analysis; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D004364:Pharmaceutical Preparations; D002216:Captopril