The effects of magnesium sulfate on fetal rats of FGR and the expression of caspase-3 in the placenta of maternal rat.
To investigate the effect of magnesium sulfate on the fetal rats of FGR and the expression of caspase-3 in the placenta of maternal rat; To explore the mechanism of using magnesium sulfate to cure the FGR. Establish model of FGR by a way of passive smoking: giving the maternal rats different agent of magnesium sulfate by subcutaneous injection: low agent group (300 mg/kg), high agent group (600 mg/kg). Concentration of magnesium sulfate was monitored. The expression of caspase-3 was measured by RT-PCR and immunohistochemistry technology. Both of the concentrations of magnesium sulfate in high and low agents group are higher than the FGR group (P < 0.01); the weight of the placenta and fetal rat in high agent group are higher than the FGR group (P < 0.05 and P < 0.01); the expression of mRNA and protein of caspase-3 in the two agent group is higher than the FGR group (P < 0.05 respectively); concentration of magnesium sulfate in the maternal rat blood correlate to the weight of fetal rat (r = 0.899, P = 0.038) and the expression of caspase-3 in the placenta of maternal rat (r = -0.747, P = 0.033; r = - 0.915, P = 0.001). The research suggests that the weight of fetal rat could be increased by treatment of magnesium sulfate. Because it would imfrmove the placental function by depressing the expression of caspase-3.
Gao
Hui
H
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zou
Li
L
D000818:Animals; D053148:Caspase 3 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005317:Fetal Growth Retardation / Q000139:chemically induced / Q000188:drug therapy*; D008278:Magnesium Sulfate / Q000627:therapeutic use*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012907:Smoking
D012333:RNA, Messenger; D008278:Magnesium Sulfate; D053148:Caspase 3