Fluorescent image analysis of lipid hydroperoxides in fish muscle with 3-perylene diphenylphosphine.
A fluorescent image analysis method was developed to evaluate lipid hydroperoxide formation in fish muscle. The lipid hydroperoxides generated in white and dark fish muscles during storage at 5-6 degrees C oxidized 3-perylene diphenylphosphine located in the tissue to yield the fluorescent derivative, 3-perylene diphenylphosphine oxide (3-PeDPPO). 3-PeDPPO thus obtained was determined by digital fluorescent image analysis. The 3-PeDPPO fluorescence intensity of white and dark muscle increased during low-temperature storage (0-24 h) and was clearly correlated with total lipid hydroperoxide levels in muscle extracts, which were determined by using HPLC based on a triphenylphosphine oxidation method (R2 = 0.954). These results suggest that 3-PeDPPO fluorescence, coupled with fluorescent image analysis, is a novel tool for direct determination of lipid hydroperoxides in fish muscle without a need for extraction of lipid.
Chotimarkorn
Chatchawan
C
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan 108-8477.
Ohshima
Toshiaki
T
Ushio
Hideki
H
D000818:Animals; D005396:Fish Products; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives*; D010720:Phosphines
C504951:3-perylene diphenylphosphine; D005456:Fluorescent Dyes; D008054:Lipid Peroxides; D010720:Phosphines; D010569:Perylene