title: CHOP is involved in neuronal apoptosis induced by neurotrophic factor deprivation.
abstract: Neurotrophic factors are essential for the survival of neurons. We found that the endoplasmic reticulum (ER) stress-C/EBP homologues protein (CHOP) pathway to be activated during neurotrophic factor deprivation-induced apoptosis in PC12 neuronal cells and in primary cultured neurons, and this apoptosis was suppressed in the neurons from chop(-/-) mice. In addition, we found that CHOP is expressed in the subventricular zone (SVZ) and striatum of the young adult mouse brain. The number of apoptotic cells in the SVZ decreased in chop(-/-) mice. These results indicate that the ER stress-CHOP pathway plays a role in neuronal apoptosis during the development of the brain.
authors.affiliation: Department of Molecular Genetics, Graduate School of Medical Sciences, Kumamoto University, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Tajiri
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Yano
authors.forename: Motohiro
authors.initials: M
authors.lastname: Morioka
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kuratsu
authors.forename: Masataka
authors.initials: M
authors.lastname: Mori
authors.forename: Tomomi
authors.initials: T
authors.lastname: Gotoh
mesh_terms: D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D017931:DNA Primers; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051379:Mice; D009414:Nerve Growth Factors / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051742:Transcription Factor CHOP / Q000502:physiology*
chemical_list: D017931:DNA Primers; D009414:Nerve Growth Factors; D051742:Transcription Factor CHOP