Interaction of subtilisin BPN' and recombinant fungal protease inhibitor F from silkworm with substituted P1 site residues.
Fungal protease inhibitor F (FPI-F) from silkworm inhibits subtilisin and fungal proteases. FPI-F mutants P(1) residues of which, Thr(29), were replaced with Glu, Phe, Gly, Leu, Met, and Arg, were prepared. The inhibitory activities of mutated FPI-F against subtilisin and other mammalian proteases indicated that FPI-F might be a specific inhibitor toward subtilisin-type protease.
Taniguchi
Mai
M
Department of Applied Biology, Kyoto Institute of Technology, Japan.
Atiwetin
Panida
P
Hirai
Tomoya
T
Itoh
Masanobu
M
Harada
Shigeharu
S
Hara
Saburo
S
Kamei
Kaeko
K
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D012831:Bombyx / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D011480:Protease Inhibitors / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics; D013381:Subtilisins / Q000037:antagonists & inhibitors* / Q000737:chemistry*
D019476:Insect Proteins; D011480:Protease Inhibitors; D011994:Recombinant Proteins; D013381:Subtilisins