Structure of the O-polysaccharide of Proteus mirabilis CCUG 10705 (OF) containing an amide of D-galacturonic acid with L-alanine.
The structure of the O-polysaccharide of Proteus mirabilis CCUG 10705 (OF) was determined by chemical analyses along with one- and two-dimensional (1)H and (13)C NMR spectroscopy. The polysaccharide was found to contain an amide of D-galacturonic acid with L-alanine and based on the uniqueness of the O-polysaccharide structure and serological data, it was suggested to classify P. mirabilis OF into a new separate Proteus serogroup, O74. A weak cross-reactivity of P. mirabilis OF and P. mirabilis O5 was observed and accounted for by a similarity of their O-repeating units. The following structure of the polysaccharide of P. mirabilis OF was established: [chemical structure: see text]
Perepelov
Andrei V
AV
ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation. perepel@ioc.ac.ru
Zabłotni
Agnieszka
A
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
Sidorczyk
Zygmunt
Z
D000107:Acetylation; D000409:Alanine / Q000737:chemistry*; D000577:Amides / Q000737:chemistry; D000818:Animals; D002240:Carbohydrate Sequence; D006603:Hexuronic Acids / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011513:Proteus mirabilis / Q000737:chemistry* / Q000145:classification; D011817:Rabbits; D012703:Serotyping
D000577:Amides; D006603:Hexuronic Acids; D008070:Lipopolysaccharides; D019081:O Antigens; C007819:galacturonic acid; D000409:Alanine; D000432:Methanol