title: Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages.
abstract: The microsporidian Encephalitozoon cuniculi is a unicellular obligate intracellular parasite considered as an emerging opportunistic human pathogen. The differentiation phase of its life cycle leads to the formation of stress-resistant spores. The E. cuniculi genome (2.9 Mbp) having been sequenced, we undertook a descriptive proteomic study of a spore-rich cell population isolated from culture supernatants. A combination of 2-DE and 2-DE-free techniques was applied to whole-cell protein extracts. Protein identification was performed using an automated MALDI-TOF-MS platform and a nanoLC-MS/MS instrument. A reference 2-DE map of about 350 major spots with multiple isoforms was obtained, and for the first time in microsporidia, a large set of unique proteins (177) including proteins with unknown function in a proportion of 25.6% was identified. The data are mainly discussed with reference to secretion and spore structural features, energy and carbohydrate metabolism, cell cycle control and parasite survival in the environment.
authors.affiliation: Equipe Parasitologie Moléculaire et Cellulaire, LBP, UMR CNRS 6023, Université Blaise Pascal, Aubière, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Brosson
authors.forename: Lauriane
authors.initials: L
authors.lastname: Kuhn
authors.forename: Frédéric
authors.initials: F
authors.lastname: Delbac
authors.forename: Jérôme
authors.initials: J
authors.lastname: Garin
authors.forename: Christian
authors.initials: C
authors.lastname: P Vivarès
authors.forename: Catherine
authors.initials: C
authors.lastname: Texier
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002460:Cell Line; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional; D009670:Encephalitozoon cuniculi / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D016890:Encephalitozoonosis / Q000662:veterinary; D005656:Fungal Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D013058:Mass Spectrometry; D010446:Peptide Fragments; D010449:Peptide Mapping; D025941:Protein Interaction Mapping; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D033761:Spores, Protozoan / Q000737:chemistry* / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D005656:Fungal Proteins; D010446:Peptide Fragments; D020543:Proteome; D011994:Recombinant Proteins; D014357:Trypsin