A B-lectin receptor kinase gene conferring rice blast resistance.
Rice blast, caused by the fungal pathogen Magnaporthe grisea, is one of the most devastating diseases in rice worldwide. The dominant resistance gene, Pi-d2 [previously named Pi-d(t)2], present in the rice variety Digu, confers gene-for-gene resistance to the Chinese blast strain, ZB15. Pi-d2 was previously mapped close to the centromere of chromosome 6. In this study, the Pi-d2 gene was isolated by a map-based cloning strategy. Pi-d2 encodes a receptor-like kinase protein with a predicted extracellular domain of a bulb-type mannose specific binding lectin (B-lectin) and an intracellular serine-threonine kinase domain. Pi-d2 is a single-copy gene that is constitutively expressed in the rice variety Digu. Transgenic plants carrying the Pi-d2 transgene confer race-specific resistance to the M. grisea strain, ZB15. The Pi-d2 protein is plasma membrane localized. A single amino acid difference at position 441 of Pi-d2 distinguishes resistant and susceptible alleles of rice blast resistance gene Pi-d2. Because of its novel extracellular domain, Pi-d2 represents a new class of plant resistance genes.
Chen
Xuewei
X
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Shang
Junjun
J
Chen
Dexi
D
Lei
Cailin
C
Zou
Yan
Y
Zhai
Wenxue
W
Liu
Guozhen
G
Xu
Jichen
J
Ling
Zhongzhuan
Z
Cao
Gang
G
Ma
Bingtian
B
Wang
Yuping
Y
Zhao
Xianfeng
X
Li
Shigui
S
Zhu
Lihuang
L
D000595:Amino Acid Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D018628:Gene Dosage; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000032:analysis; D007113:Immunity, Innate / Q000235:genetics; D020082:Magnaporthe / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000032:analysis; D017422:Sequence Analysis, DNA
D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D017346:Protein Serine-Threonine Kinases