Comparative analysis of purified Pacific and Caribbean ciguatoxin congeners and related marine toxins using a modified ELISA technique.
The monoclonal antibody to ciguatoxin (CTX) produced from a hybridoma cell line was assayed for the detection of four congeners of CTX: Pacific ciguatoxin-1 (P-CTX-1), Pacific ciguatoxin-2 (P-CTX-2), Pacific ciguatoxin-3 (P-CTX-3), and Caribbean ciguatoxin-1 (C-CTX-1) and related marine toxins, including domoic acid, palytoxin, and okadaic acid, using a modified enzyme-linked immunosorbent assay (ELISA). Lower detection limits were assessed and linearity was statistically established (P<0.05) for P-CTX-1, P-CTX-2, and P-CTX-3 and C-CTX-1 at concentrations ranging from 0 to 5.00 ng, while the other marine toxins showed statistically insignificant cross-reactivities at similar concentrations. Thus, the monoclonal antibody to CTX is able to specifically detect various CTX congeners at levels comparable to those naturally occurring in ciguatoxic fish.
Campora
Cara E
CE
Department of Cell and Molecular Biology, University of Hawaii-Manoa, Honolulu, Hawaii, USA. empey@hawaii.edu
Hokama
Y
Y
Ebesu
Joanne S M
JS
D000178:Acrylamides / Q000032:analysis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis; D017691:Caribbean Region; D036841:Ciguatera Poisoning; D002922:Ciguatoxins / Q000032:analysis* / Q000276:immunology; D003064:Cnidarian Venoms; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007608:Kainic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000276:immunology; D019319:Okadaic Acid / Q000032:analysis / Q000276:immunology; D010140:Pacific Ocean; D017747:Seafood / Q000032:analysis; D000095702:Polyether Toxins
D000178:Acrylamides; D000911:Antibodies, Monoclonal; D002922:Ciguatoxins; D003064:Cnidarian Venoms; D007608:Kainic Acid; D019319:Okadaic Acid; C012301:domoic acid; C010272:palytoxin; D000095702:Polyether Toxins