Interactions between yeast lees and wine polyphenols during simulation of wine aging. II. Analysis of desorbed polyphenol compounds from yeast lees.
In the first part of this work, the analysis of the polyphenolic compounds remaining in the wine after different contact times with yeast lees during simulation of red wine aging was undertaken. To achieve a more precise view of the wine polyphenols adsorbed on lees during red wine aging and to establish a clear balance between adsorbed and remnant polyphenol compounds, the specific analysis of the chemical composition of the adsorbed polyphenolic compounds (condensed tannins and anthocyanins) after their partial desorbtion from yeast lees by denaturation treatments was realized in the second part of the study. The total recovery of polyphenol compounds from yeast lees was not complete, since a rather important part of the initial wine colored polyphenols, especially those with a dominant blue color component, remained strongly adsorbed on yeast lees, as monitored by color tristimulus and reflectance spectra measurements. All anthocyanins were recovered at a rather high percentage (about 62%), and it was demonstrated that they were not adsorbed in relation with their sole polarity. Very few monomeric phenolic compounds were extracted from yeast lees. With the use of drastic denaturing treatments, the total recovery of condensed tannins reached 83%. Such tannins extracted from yeast lees exhibited very high polymeric size and a rather high percentage of galloylated residues by comparison with initial wine tannins, indicating that nonpolar tannins were preferentially desorbed from yeast lees by the extraction treatments.
Mazauric
Jean-Paul
JP
Unité Mixte de Recherches, Sciences pour l'Oenologie, INRA, 2 place Viala, F-34060 Montpellier Cedex 1, France.
Salmon
Jean-Michel
JM
D000327:Adsorption; D000872:Anthocyanins / Q000032:analysis; D005419:Flavonoids / Q000032:analysis* / Q000378:metabolism; D005511:Food Handling / Q000379:methods; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D059808:Polyphenols; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism; D013634:Tannins / Q000032:analysis; D013997:Time Factors; D014920:Wine / Q000032:analysis*
D000872:Anthocyanins; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013634:Tannins