title: [Primary study on proteomics about Ganoderma lucidium spores promoting survival and axon regeneration of injured spinal motor neurons in rats].
abstract: OBJECTIVE
  To detect some proteins associated with the effect of ganoderma lucidium spores (GASP) on promoting the survival and axon regeneration of injured spinal motor neurons in rats.
  
  
  METHODS
  The rats were divided into normal control group, untreated group and GASP-treated group, and the rats in the last two groups received ventral root avulsion. GASP preparation was fed to the rats in the GASP-treated group for 14 days. The gray matter tissues of the lumbar spinal were sampled from rats in each group after 14 days following ventral root avulsion, and the extracted proteins from these tissues were detected by using 2-dimensional electrophoresis. Matrix assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS) was utilized to identify the differentially expressed proteins among these three groups.
  
  
  RESULTS
  There were six kinds of proteins differentially expressed among the three groups, which were collapsin response mediator protein 2 (CRMP-2), F-actin capping protein beta subunit (FCP-beta), isocitrate dehydrogenase [NAD] subunit beta (IDH-beta), ATPase, glutamate oxaloacetate transaminase-1 (GOT1) and M2 pyruvate kinase (M2-PK). The expression levels of CRMP-2, IDH-beta, ATPase and GOT1 were higher in the GASP-treated group than those in the untreated group, while the expression levels of FCP-beta and M2-PK were lower than those in the untreated group.
  
  
  CONCLUSIONS
  GASP maybe promotes the survival and axon regeneration of injured spinal motor neurons in rats by virtue of up- or down-regulating the expression levels of the proteins mentioned above.
authors.affiliation: Division of Neuroscience, Department of Histology and Embryology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yuan-Shan
authors.initials: YS
authors.lastname: Zeng
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Cheng
authors.forename: Sui-Jun
authors.initials: SJ
authors.lastname: Chen
mesh_terms: D051344:Actin Capping Proteins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D001369:Axons / Q000502:physiology; D002470:Cell Survival; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use; D036341:Intercellular Signaling Peptides and Proteins; D007521:Isocitrate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D009046:Motor Neurons / Q000502:physiology*; D009416:Nerve Regeneration / Q000187:drug effects*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D008517:Phytotherapy; D040901:Proteomics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020940:Reishi; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000503:physiopathology; D013172:Spores, Fungal
chemical_list: D051344:Actin Capping Proteins; D004365:Drugs, Chinese Herbal; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C095114:collapsin response mediator protein-2; D007521:Isocitrate Dehydrogenase