Effect of the cryopreservation conditions on the viability of the rumen ciliate Diploplastron (Metadinium) affine.
OBJECTIVE
To study the viability of Diploplastron (Metadinium) affine after its cryopreservation at two cooling rates, and the effect of procedure conditions on viability.


RESULTS
There were differences in viability between cooling rates (1 and 4 degrees C min(-1)) at 15 or 5 degrees C, but not after thawing. When the equilibrium temperature (25 or 5 degrees C), the cryopreservant (glycerol or dimethyl sulfoxide [DMSO]) and the use of membrane protector were tested, there were no differences caused by the cryopreservant or the membrane protector. However, the equilibrium at 25 degrees C increased the viability (P = 0.005) compared with 5 degrees C.


CONCLUSIONS
Viability after thawing was 0.10-0.19. Adding the cryopreservant (either glycerol or DMSO) at 25 degrees C instead of 5 degrees C improves viability of D. affine after thawing.


CONCLUSIONS
Conditions of cryopreservation are largely dependent on the species of rumen protozoa. Number of viable cells after thawing would indicate the possibility of culture recovery for D. affine.
de la Fuente
G
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Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Zaragoza, Spain.
Cebrián
J A
JA
Fondevila
M
M
D000818:Animals; D016798:Ciliophora / Q000187:drug effects / Q000254:growth & development*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D012417:Rumen / Q000469:parasitology*; D013696:Temperature
D003451:Cryoprotective Agents; D005990:Glycerol; D004121:Dimethyl Sulfoxide