title: 8-Isoprostane is a dose-related biomarker for photo-oxidative ultraviolet (UV) B damage in vivo: a pilot study with personal UV dosimetry.
abstract: BACKGROUND
  Ultraviolet (UV) B irradiation causes visible erythema, which has been linked with DNA damage. However, besides such direct photochemical conformation changes, UVB also induces many indirect photochemical effects in the skin. Lipid peroxidation (LPO) is in this context one of the major pathways by which photo-oxidative stress disturbs cell signalling and promotes photocarcinogenesis and photoageing. So far we lack techniques for visualizing photo-oxidative stress in the skin. Furthermore, LPO has never been linked with individually acquired UVB doses measured by personal dosimetry.
  
  
  OBJECTIVE
  Measuring the skin reaction and photo-oxidative damage by LPO in vivo after UVB exposure in a pilot study surveyed by personal dosimetry in order to allow for a correlation analysis of acquired dose, skin reaction and amount of LPO.
  
  
  METHODS
  UVB exposure was measured with the opto-electronic X2000-1 (Gigahertz Optik, Puchheim, Germany) and the biological DLR Biofilm (German Aerospace Center DLR, Cologne, Germany) portable dosimeter. The skin reaction following UVB exposure was quantified with a Minolta chromameter (Minolta, Tokyo, Japan) and LPO in vivo was measured by 8-isoprostane generation by means of densitometric analysis of immunohistochemical samples obtained 30 min post-UVB irradiation.
  
  
  RESULTS
  Regression analysis revealed significant linear relations between UVB exposures recorded by the dosimeters and colorimetry parameters of the skin reaction. Furthermore, an even better linear relation with higher significance was found between the generation of 8-isoprostane in the skin and the dosimeter readouts.
  
  
  CONCLUSIONS
  LPO measured by the generation of 8-isoprostane provides a suitable intrinsic biomarker for photo-oxidative UVB damage in vivo. This study provides a new approach to visualizing photo-oxidative stress in the skin in vivo. Furthermore, future dosimeter readouts can now be set into relation to the expected increase of LPO that can be calculated within the limits of our study.
authors.affiliation: Department of Dermatology and Allergology, University of Ulm, Maienweg 12, D-09081 Ulm, Germany.
authors.forename: L A
authors.initials: LA
authors.lastname: Schneider
authors.forename: W
authors.initials: W
authors.lastname: Bloch
authors.forename: K
authors.initials: K
authors.lastname: Kopp
authors.forename: A
authors.initials: A
authors.lastname: Hainzl
authors.forename: P
authors.initials: P
authors.lastname: Rettberg
authors.forename: M
authors.initials: M
authors.lastname: Wlaschek
authors.forename: G
authors.initials: G
authors.lastname: Horneck
authors.forename: K
authors.initials: K
authors.lastname: Scharffetter-Kochanek
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D028421:Isoprostanes / Q000096:biosynthesis*; D015227:Lipid Peroxidation / Q000528:radiation effects; D008297:Male; D018384:Oxidative Stress / Q000528:radiation effects; D010865:Pilot Projects; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000209:etiology / Q000378:metabolism*; D011874:Radiometry / Q000379:methods; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D028421:Isoprostanes