High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow.
Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l(-1). The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone.
Salamone
Daniel
D
Biosidus SA, Buenos Aires, Argentina.
Barañao
Lino
L
Santos
Claudio
C
Bussmann
Leonardo
L
Artuso
Jorge
J
Werning
Carlos
C
Prync
Aida
A
Carbonetto
Cesar
C
Dabsys
Susana
S
Munar
Carlos
C
Salaberry
Roberto
R
Berra
Guillermo
G
Berra
Ignacio
I
Fernández
Nahuel
N
Papouchado
Mariana
M
Foti
Marcelo
M
Judewicz
Norberto
N
Mujica
Ignacio
I
Muñoz
Luciana
L
Alvarez
Silvina Fenández
SF
González
Eliseo
E
Zimmermann
Juan
J
Criscuolo
Marcelo
M
Melo
Carlos
C
D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D019976:Cloning, Organism; D019382:Human Growth Hormone / Q000096:biosynthesis*; D006801:Humans; D008894:Milk Proteins / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*
D008894:Milk Proteins; D011994:Recombinant Proteins; D019382:Human Growth Hormone