title: Identifying influenza viruses with resequencing microarrays.
abstract: Identification of genetic variations of influenza viruses is essential for epidemic and pandemic outbreak surveillance and determination of vaccine strain selection. In this study, we combined a random amplification strategy with high-density resequencing microarray technology to demonstrate simultaneous detection and sequence-based typing of 25 geographically distributed human influenza virus strains collected in 2004 and 2005. In addition to identification, this method provided primary sequence information, which suggested that distinct lineages of influenza viruses co-circulated during the 2004-2005 season, and simultaneously identified and typed all component strains of the trivalent FluMist intranasal vaccine. The results demonstrate a novel, timely, and unbiased method for the molecular epidemiologic surveillance of influenza viruses.
authors.affiliation: Naval Research Laboratory, Washington, DC, USA.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Luke T
authors.initials: LT
authors.lastname: Daum
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Vora
authors.forename: David
authors.initials: D
authors.lastname: Metzgar
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Walter
authors.forename: Linda C
authors.initials: LC
authors.lastname: Canas
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Malanoski
authors.forename: Baochuan
authors.initials: B
authors.lastname: Lin
authors.forename: David A
authors.initials: DA
authors.lastname: Stenger
mesh_terms: D000595:Amino Acid Sequence; D005814:Genes, Viral / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D006388:Hemagglutinins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009981:Influenza B virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010802:Phylogeny
chemical_list: D006388:Hemagglutinins