title: Ion-exchange macroporous hydrophilic gel monolith with grafted polymer brushes.
abstract: The grafting of functional polymers to the pore surface of macroporous monolithic polyacrylamide cryogels was found to be an efficient and convenient method for the preparation of macroporous polyacrylamide gels, so-called cryogels (pAAm cryogels), with both controlled extent of functional group incorporated and with tailored surface chemistries. Anion-exchange polymer chains of poly(2-(dimethylamino)ethyl methacrylate) (pDMAEMA) and poly([2-(methacryloyloxy)ethyl]-trimethylammonium chloride) (pMETA), and cation-exchange polymer chains of polyacrylate have been grafted onto pAAm cryogels using potassium diperiodatocuprate as initiator. It was possible to achieve the ion-exchange capacity up to 0.2-0.5 mmol/ml. The graft polymerization did not alter the macroporous structure of the pAAm cryogel, however the flow rate of solutes through the cryogel matrix decreased with increase in the density of polymer grafted. The sorption of low-molecular-weight (metal ion, dye) and high-molecular-weight (protein) substances on the grafted monolithic pAAm column has been studied. The results indicate that a 'tentacle'-type binding of protein to grafted polymer depended on the architecture of the grafted polymer layer and took place after a certain degree of grafting has been reached. The binding of proteins by tentacle-like polymer chains allowed for increasing the binding capacity for proteins on the grafted pAAm cryogels up to 6-12 mg/ml.
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, SE-22100 Lund, Sweden.
authors.forename: Irina N
authors.initials: IN
authors.lastname: Savina
authors.forename: Igor Yu
authors.initials: IY
authors.lastname: Galaev
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001391:Azo Compounds / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D003300:Copper / Q000378:metabolism; D007474:Ion Exchange; D009113:Muramidase / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; D001391:Azo Compounds; D011108:Polymers; C016680:polyacrylamide gels; C008710:Orange G; D012710:Serum Albumin, Bovine; D003300:Copper; D009113:Muramidase