title: Proteomic analysis of UVC irradiation-induced damage of plasma proteins: Serum amyloid P component as a major target of photolysis.
abstract: Ultraviolet-C (UVC) irradiation is a pathogen inactivation method used for disinfection of pharmaceutical products derived from human blood. Previous studies have shown that UVC can potentially damage proteins through photolysis or can generate reactive species resulting in protein thiol oxidation. In this study, two fluorescence-based quantitative proteomic approaches were used to assess the effects of a novel UVC-disinfection strategy on human plasma fractions. We show minimal changes in protein content, but gross alterations in protein thiol reactivity, indicative of oxidative damage. We identify a number of the damaged proteins by mass spectrometry, including serum amyloid P component, and further demonstrate UVC-induced photolysis of its disulphide bond.
authors.affiliation: Ludwig Institute for Cancer Research and Department of Biochemistry and Molecular Biology, University College London, Cruciform Building, Gower Street, London WC1E 6BT, UK.
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Chan
authors.forename: Piers R
authors.initials: PR
authors.lastname: Gaffney
authors.forename: Michael D
authors.initials: MD
authors.lastname: Waterfield
authors.forename: Heinz
authors.initials: H
authors.lastname: Anderle
authors.forename: H
authors.initials: H
authors.lastname: Peter Matthiessen
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Schwarz
authors.forename: Peter L
authors.initials: PL
authors.lastname: Turecek
authors.forename: John F
authors.initials: JF
authors.lastname: Timms
mesh_terms: D001798:Blood Proteins / Q000032:analysis / Q000528:radiation effects*; D004203:Disinfection / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry; D018384:Oxidative Stress; D010782:Photolysis; D040901:Proteomics; D000683:Serum Amyloid P-Component / Q000032:analysis / Q000528:radiation effects*; D013438:Sulfhydryl Compounds / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D001798:Blood Proteins; D000683:Serum Amyloid P-Component; D013438:Sulfhydryl Compounds