title: Treatment and transfer of emphysema by a new bone marrow transplantation method from normal mice to Tsk mice and vice versa.
abstract: We have recently established a new bone marrow transplantation (BMT) method in which bone marrow cells are injected into the intrabone marrow (IBM). In the present study, we used an animal model for emphysema (tight-skin [Tsk] mouse) to examine whether IBM-BMT could be used to treat emphysema in Tsk mice. IBM-BMT was carried out from C3H mice into Tsk mice (8-10 weeks old) that had already shown emphysema. Six months after transplantation, the lungs of all the Tsk mice treated with IBM-BMT [C3H-->Tsk] showed similar structures to those of normal mice, whereas the [Tsk-->Tsk] mice showed emphysema, as seen in age-matched Tsk mice. Next, we attempted to transfer emphysema from Tsk mice to C3H mice by IBM-BMT. Six months after IBM-BMT, the [Tsk-->C3H] mice showed emphysema. These results strongly suggest that emphysema in Tsk mice originates from defects of stem cells in the bone marrow.
authors.affiliation: First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan, 570-0023.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Adachi
authors.forename: Haruki
authors.initials: H
authors.lastname: Oyaizu
authors.forename: Shigeru
authors.initials: S
authors.lastname: Taketani
authors.forename: Keizo
authors.initials: K
authors.lastname: Minamino
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Leonard D
authors.initials: LD
authors.lastname: Shultz
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Iwasaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Tomita
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Keiji
authors.initials: K
authors.lastname: Nakano
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Koike
authors.forename: Ryoji
authors.initials: R
authors.lastname: Yasumizu
authors.forename: Makoto
authors.initials: M
authors.lastname: Sata
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Hirama
authors.forename: Isao
authors.initials: I
authors.lastname: Kubota
authors.forename: Shirou
authors.initials: S
authors.lastname: Fukuhara
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods*; D046528:Chimerism; D004646:Emphysema / Q000628:therapy*; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D006413:Hematopoietic System / Q000166:cytology; D008168:Lung / Q000166:cytology / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011505:Protein-Tyrosine Kinases / Q000172:deficiency*; D012867:Skin / Q000166:cytology / Q000473:pathology
chemical_list: D011505:Protein-Tyrosine Kinases; C080813:emt protein-tyrosine kinase