title: Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste.
abstract: The aim of this work was to study chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. The culture produced two biosurfactants, a and b, which showed strong activity and were identified as L-rhamnopyranosyl-L-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydecanoate or Rha-Rha C10-C10 and L-rhamnopyranosyl-L-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydodecanoate or Rha-Rha C(10)-C(12), respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as SDS and Tween 80. Rhamnolipid a showed significant antiproliferative activity against human breast cancer cell line (MCF-7) at minimum inhibitory concentration (MIC) at 6.25 microg/mL while rhamnolipid b showed MIC against insect cell line C6/36 at 50 microg/mL.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand. benjamat@swu.ac.th
authors.forename: Benjamas
authors.initials: B
authors.lastname: Thanomsub
authors.forename: Wanna
authors.initials: W
authors.lastname: Pumeechockchai
authors.forename: Anirut
authors.initials: A
authors.lastname: Limtrakul
authors.forename: Panarat
authors.initials: P
authors.lastname: Arunrattiyakorn
authors.forename: Wipawan
authors.initials: W
authors.lastname: Petchleelaha
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Nitoda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kanzaki
mesh_terms: D000330:Aedes; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000032:analysis*; D002460:Cell Line; D003611:Dairy Products / Q000382:microbiology; D006017:Glycolipids / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D015394:Molecular Structure; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D006017:Glycolipids; D013501:Surface-Active Agents; C418382:rhamnolipid