title: A keratin cytoskeletal protein regulates protein synthesis and epithelial cell growth.
abstract: Cell growth, an increase in mass and size, is a highly regulated cellular event. The Akt/mTOR (mammalian target of rapamycin) signalling pathway has a central role in the control of protein synthesis and thus the growth of cells, tissues and organisms. A striking example of a physiological context requiring rapid cell growth is tissue repair in response to injury. Here we show that keratin 17, an intermediate filament protein rapidly induced in wounded stratified epithelia, regulates cell growth through binding to the adaptor protein 14-3-3sigma. Mouse skin keratinocytes lacking keratin 17 (ref. 4) show depressed protein translation and are of smaller size, correlating with decreased Akt/mTOR signalling activity. Other signalling kinases have normal activity, pointing to the specificity of this defect. Two amino acid residues located in the amino-terminal head domain of keratin 17 are required for the serum-dependent relocalization of 14-3-3sigma from the nucleus to the cytoplasm, and for the concomitant stimulation of mTOR activity and cell growth. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis.
authors.affiliation: Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: Seyun
authors.initials: S
authors.lastname: Kim
authors.forename: Pauline
authors.initials: P
authors.lastname: Wong
authors.forename: Pierre A
authors.initials: PA
authors.lastname: Coulombe
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D000818:Animals; D048708:Cell Growth Processes; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D004475:Ectoderm / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D007633:Keratins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000378:metabolism; D021381:Protein Transport; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases
chemical_list: D048948:14-3-3 Proteins; D007633:Keratins; D011494:Protein Kinases; C546843:mTOR protein, mouse; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases