[Detection of MSH2 gene mutation by PCR].
OBJECTIVE
To establish a genetic diagnosis method for a novel MSH2 mutation.


METHODS
A specific primer on the mutated site of MSH2 was synthesized and PCR was conducted using the specific primer and another downstream primer. PCR products were electrophoresed and then the carriers with the novel gene mutation of the carriers or non-carriers were identified.


RESULTS
MSH2 in a hereditary nonpolyposis colorectal cancer family were successfully found.


CONCLUSIONS
The method is effective and simple for genetic diagnosis of the novel mutation in MSH2.
Zheng
Duo
D
School of Biologic Science and Technology, Central South University, Changsha, China. zhengduo@hotmail.com
Liu
Xiao-ping
XP
Li
Tie-gang
TG
Li
Jun
J
Tang
Li-jun
LJ
Hu
Wei-xin
WX
D001483:Base Sequence; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D051718:MutS Homolog 2 Protein / Q000235:genetics*; D010375:Pedigree; D017354:Point Mutation; D016133:Polymerase Chain Reaction
D051718:MutS Homolog 2 Protein