Study of V1a vasopressin receptor gene single nucleotide polymorphisms in platelet vasopressin responsiveness.
There is a significant heterogeneity among individuals in terms of platelet aggregation response to arginine vasopressin (AVP). The aim of this study was to evaluate whether four single nucleotide polymorphisms (SNPs) in the promoter region of vasopressin V1a receptor gene (V1aR) could be used as genetic markers for divergent platelet aggregation response to AVP. Seventeen of 33 subjects showed more than 60% of maximum platelet aggregation and were classified as responders. Sixteen were classified as nonresponders because they had less than 30% aggregation. In a preliminary study, V1aR gene sequences were determined in two responders and two nonresponders. We found four SNPs in the promoter region of the V1aR gene: -6951G/A, -4112A/T, -3860T/C, and -242C/T. In all 33 subjects the genotypes of four SNPs were determined using either polymerase chain reaction (PCR) with allele-specific primers or PCR followed by restriction-fragment length polymorphism (RFLP). There were no differences in the AVP-induced aggregation between the subjects with and without variant alleles of each four SNPs. The genotype frequencies of four SNPs of V1aR were almost identical between AVP responders and nonresponders. These results suggest that the four SNPs in the promoter region of the V1aR gene may not be useful as genetic markers for platelet aggregation heterogeneity.
Hasan
Kazi N
KN
Department of Laboratory Medicine, Hirosaki University School of Medicine, Hirosaki University, Hirosaki, Japan.
Shoji
Masaru
M
Tsutaya
Shoji
S
Kudo
Ryoko
R
Matsuda
Eriko
E
Saito
Junko
J
Kimura
Tokihisa
T
Yasujima
Minoru
M
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001127:Arginine Vasopressin / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D004338:Drug Combinations; D005819:Genetic Markers; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017483:Receptors, Vasopressin / Q000235:genetics*
D004338:Drug Combinations; D005819:Genetic Markers; D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; D000244:Adenosine Diphosphate