Importance of virus-medium interactions on the biological activity of wild-type Heliothine nucleopolyhedroviruses propagated via suspension insect cell cultures.
In vitro serial passaging of nucleopolyhedroviruses often results in virus instability, leading to reduction of both yield and biological activity of polyhedra (virus occlusion bodies). In this study, uncloned Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HaSNPV) and cloned Helicoverpa zea single-nucleocapsid nucleopolyhedrovirus (HzSNPV) were each serially passaged five times in both low cost (VPM) and commercial (Excell 401) media using H. zea cell cultures. When the experimental data was analysed as a 2(3) full factorial design (testing two levels of virus, medium and passage number), the passage number was shown to have the most significant effect on polyhedra yield, while the virus-medium interaction had the most important effect on polyhedra biological activity. This interaction was most pronounced for HzSNPV, which experienced a dramatic decline in biological activity when switched from its original Excell 401 medium to the in-house VPM medium. This suggests that genetically homogeneous viruses are less able to adapt to perturbations in the nutrient environment, hence medium changes should be avoided. Therefore, it is important to standardise the scale-up process of nucleopolyhedrovirus biopesticides at the earliest stage of development, especially when it requires both a low cost medium and a plaque purified virus.
Pedrini
Márcia R S
MR
Division of Chemical Engineering, The University of Queensland, St. Lucia, QLD 4072, Australia.
Christian
Peter
P
Nielsen
Lars K
LK
Reid
Steven
S
Chan
Leslie C L
LC
D000818:Animals; D002460:Cell Line; D003470:Culture Media; D007313:Insecta; D015999:Multivariate Analysis; D017924:Nucleopolyhedroviruses / Q000254:growth & development / Q000502:physiology*; D014776:Virus Cultivation
D003470:Culture Media