Interaction of nucleoside diphosphate kinase and catalases for stress and light responses in Neurospora crassa.
Nucleoside diphosphate kinase (NDK) is an ubiquitous enzyme with the function of a signal transducer. In Neurospora crassa, an ndk-1(P72H) mutant carrying the point mutation Pro72His was isolated. We found that ndk-1(P72H) showed hypersensitivity to oxidative and heat stress and a decrease in the levels of catalase (Cat)-1 and -3 induced by oxidative, heat stress and illumination compared with wild type (Wt). We found, by conducting a yeast two-hybrid assay, that Cat-1 interacted with NDK-1. NDK-1 was suggested to control Cat-1 and Cat-3 at the post-transcriptional level in response to heat, oxidative stress and light.
Yoshida
Yusuke
Y
Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Totsuka-ku, Yokohama 244-0813, Japan.
Ogura
Yasunobu
Y
Hasunuma
Kohji
K
D002374:Catalase / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D047468:Immunoprecipitation; D008027:Light; D009492:Neurospora crassa / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D025941:Protein Interaction Mapping; D020798:Two-Hybrid System Techniques; D014867:Water / Q000494:pharmacology
D005656:Fungal Proteins; D014867:Water; D002374:Catalase; D009701:Nucleoside-Diphosphate Kinase