Cartilage regeneration using mesenchymal stem cells and a PLGA-gelatin/chondroitin/hyaluronate hybrid scaffold.
The study was to produce a novel hybrid poly-(lactic-co-glycolic acid) (PLGA)-gelatin/chondroitin/hyaluronate (PLGA-GCH) scaffold and evaluate its potentials in cartilage repair. The porous PLGA-GCH scaffold was developed to mimic the natural extra cellular matrix of cartilage. The differentiated mesenchymal stem cells (MSCs) seeded on PLGA-GCH or PLGA scaffold were incubated in vitro and showed that, compared to PLGA scaffold, the PLGA-GCH scaffold significantly augmented the proliferation of MSCs and GAG synthesis. Then autologous differentiated MSCs/PLGA-GCH was implanted to repair full-thickness cartilage defect in rabbit, while MSCs/PLGA for the contra lateral cartilage defect (n=30). Fifteen additional rabbits without treatment for defects were used as control. Histology observation showed the MSCs/PLGA-GCH repair group had better chondrocyte morphology, integration, continuous subchondral bone, and much thicker newly formed cartilage compared with MSCs/PLGA repair group 12 and 24 weeks postoperatively. There was a significant difference in histological grading score between these two groups, which both showed much better repair than control. The present study implied that the hybrid PLGA-GCH scaffold might serve as a new way to keep the differentiation of MSCs for enhancing cartilage repair.
Fan
Hongbin
H
Institute of Orthopaedics & Traumatology, Xijing Hospital, the Fourth Military Medical University, PR China.
Hu
Yunyu
Y
Zhang
Chunli
C
Li
Xusheng
X
Lv
Rong
R
Qin
Ling
L
Zhu
Rui
R
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002358:Cartilage, Articular / Q000166:cytology / Q000502:physiology*; D002807:Chondroitin / Q000031:analogs & derivatives* / Q000737:chemistry; D048091:Guided Tissue Regeneration / Q000379:methods; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology; D011098:Polyglactin 910 / Q000737:chemistry*; D011817:Rabbits; D012038:Regeneration
D001672:Biocompatible Materials; C513251:poly-(lactic-co-glycolic acid)-gelatin chondroitin hyaluronate; D011098:Polyglactin 910; D006820:Hyaluronic Acid; D002807:Chondroitin