Microarray image scanning.
Of the technologies available for measuring gene expression, microarrays using cDNA targets is one of the most common and well-developed high-throughput techniques. With this technique, the expression levels of thousands of genes are measured simultaneously. DNA probes are immobilized on solid surfaces, either membrane-based or chemically coated glass surfaces. On glass arrays, the probes are hybridized with fluorescent-labeled target samples. Fluorescence intensities, which reflect gene expression levels, are detected by imaging the array using a laser or white-light source and capturing the image using photomultiplier tube detection or a charge-coupled device camera. Different laser-based scanners are used in laboratories to scan microarray images. This chapter discusses the imaging process and the protocols being developed.
Ramdas
Latha
L
Cancer Genomics Core Laboratory, University of Texas, M.D. Anderson Cancer Center, Houston, USA.
Zhang
Wei
W
D002232:Carbocyanines / Q000378:metabolism; D015342:DNA Probes / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D013223:Statistics as Topic
D002232:Carbocyanines; D015342:DNA Probes; D005456:Fluorescent Dyes