title: The in vivo metabolism of 3-oxo-5-benzylidene-6-methyl-(4H)-2-(benzoylmethyl)pyridazine in rats.
abstract: The purpose of the study was to investigate the in vivo metabolic pathway of 3-oxo-5-benzylidene-6-methyl-(4H)-2-(benzoylmethyl)pyridazine (substrate) in rats. Firstly its potential metabolites, i.e. N-dealkylation, ring scission of pyridazine and aromatic hydroxylation products, were synthesized and then the substrate was given orally (100 mg/kg) to male or female Wistar rats at a dose of 100 mg/kg to body weight. Blood samples were collected at 0, 1, 2, 4, 6 and 8 hours after administration of substrate and blood was centrifuged to obtain serum. The substrate and its potential metabolites were separated using a gradient HPLC method on a reverse phase system. This study revealed that 3-oxo-5-benzylidene-6-methyl-(4H)-2-(benzoylmethyl)pyridazine was not metabolized to the proposed metabolites and was present unchanged in the serum.
authors.affiliation: Marmara University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Istanbul, Turkey.
authors.forename: E Elçin
authors.initials: EE
authors.lastname: Oruç
authors.forename: Oya
authors.initials: O
authors.lastname: Unsal
authors.forename: Ayla
authors.initials: A
authors.lastname: Balkan
authors.forename: Fügen
authors.initials: F
authors.lastname: Ozkanli
authors.forename: M Zafer
authors.initials: MZ
authors.lastname: Gören
authors.forename: Berna
authors.initials: B
authors.lastname: Terzioğlu
authors.forename: Sevim
authors.initials: S
authors.lastname: Rollas
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood / Q000138:chemical synthesis / Q000378:metabolism*; D001711:Biotransformation; D002406:Catheterization, Peripheral; D002851:Chromatography, High Pressure Liquid; D003640:Dealkylation; D005260:Female; D006900:Hydroxylation; D007083:Iliac Artery; D008297:Male; D011724:Pyridazines / Q000097:blood / Q000138:chemical synthesis / Q000378:metabolism*; D051381:Rats; D012015:Reference Standards; D012996:Solutions
chemical_list: C511936:3-oxo-5-benzylidene-6-methyl-(4H)-2-(benzoylmethyl)pyridazine; D000894:Anti-Inflammatory Agents, Non-Steroidal; D011724:Pyridazines; D012996:Solutions