title: A screen for genes of heme uptake identifies the FLC family required for import of FAD into the endoplasmic reticulum.
abstract: Although Candida albicans and Saccharomyces cerevisiae express very similar systems of iron uptake, these species differ in their capacity to use heme as a nutritional iron source. Whereas C. albicans efficiently takes up heme, S. cerevisiae grows poorly on media containing heme as the sole source of iron. We identified a gene from C. albicans that would enhance heme uptake when expressed in S. cerevisiae. Overexpression of CaFLC1 (for flavin carrier 1) stimulated the growth of S. cerevisiae on media containing heme iron. In C. albicans, deletion of both alleles of CaFLC1 resulted in a decrease in heme uptake activity, whereas overexpression of CaFLC1 resulted in an increase in heme uptake. The S. cerevisiae genome contains three genes with homology to CaFLC1, and two of these, termed FLC1 and FLC2, also stimulated growth on heme when overexpressed in S. cerevisiae. The S. cerevisiae Flc proteins were detected in the endoplasmic reticulum and the FLC genes encoded an essential function, as strains deleted for either FLC1 or FLC2 were viable, but deletion of both FLC1 and FLC2 was synthetically lethal. FLC gene deletion resulted in pleiotropic phenotypes related to defects in cell wall integrity. High copy suppressors of this synthetic lethality included three mannosyltransferases, VAN1, KTR4, and HOC1. FLC deletion strains exhibited loss of cell wall mannose phosphates, defects in cell wall assembly, and delayed maturation of carboxypeptidase Y. Permeabilized cells lacking FLC proteins exhibited dramatic loss of FAD import activity. We propose that the FLC genes are required for import of FAD into the lumen of the endoplasmic reticulum, where it is required for disulfide bond formation.
authors.affiliation: Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: Olga
authors.initials: O
authors.lastname: Protchenko
authors.affiliation: Department of Biology, McGill University, Montreal, Quebec H3A 1B1, Canada.
authors.forename: Roberto
authors.initials: R
authors.lastname: Rodriguez-Suarez
authors.affiliation: Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: Rachel
authors.initials: R
authors.lastname: Androphy
authors.affiliation: Department of Biology, McGill University, Montreal, Quebec H3A 1B1, Canada.
authors.forename: Howard
authors.initials: H
authors.lastname: Bussey
authors.affiliation: Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892. Electronic address: carolinep@intra.niddk.nih.gov.
authors.forename: Caroline C
authors.initials: CC
authors.lastname: Philpott
mesh_terms: D000483:Alleles; D002176:Candida albicans / Q000378:metabolism; D043402:Cathepsin A / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000493:pharmacokinetics*; D005656:Fungal Proteins / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005821:Genetic Techniques; D006418:Heme / Q000737:chemistry / Q000493:pharmacokinetics*; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D004220:Disulfides; D005656:Fungal Proteins; D005182:Flavin-Adenine Dinucleotide; D006418:Heme; D043402:Cathepsin A