Hydrolysis of black soybean isoflavone glycosides by Bacillus subtilis natto.
Hydrolysis of isoflavone glycosides by Bacillus subtilis natto NTU-18 in black soymilk is reported. At the concentration of 3-5% (w/v), black soymilk in flask cultures, the isoflavones, daidzin, and genistin were highly deglycosylated within 24 h. Deglycosylation of isoflavones was further carried out in a 7-l fermenter with 5% black soymilk. During the fermentation, viable cells increased from 10(3) to 10(9) CFU ml(-1) in 15 h, and the activity of beta-glucosidase appeared at 8 h after inoculation and reached a maximum (3.3 U/ml) at 12 h, then decreased rapidly. Deglycosylation of isoflavone glycosides was observed at the same period, the deglycosylation rate of daidzin and genistin at 24 h was 100 and 75%, respectively. It is significantly higher than the previous reports of fermentation with lactic acid bacteria. In accordance with the deglycosylation of isoflavone glycosides, the estrogenic activity of the 24 h fermented black soymilk for ERbeta estrogen receptor increased to threefold; meanwhile, the fermented broth activated ERalpha estrogen receptor to a less extent than ERbeta. These results suggest that this fermentation effectively hydrolyzed the glycosides from isoflavone in black soymilk and the fermented black soymilk has the potential to be applied to selective estrogen receptor modulator products.
Kuo
Lun-Cheng
LC
Institute of Microbiology and Biochemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan, Republic of China.
Cheng
Wei-Yi
WY
Wu
Ren-Yu
RY
Huang
Ching-Jang
CJ
Lee
Kung-Ta
KT
D001412:Bacillus subtilis / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004967:Estrogens / Q000378:metabolism; D005285:Fermentation; D006027:Glycosides / Q000737:chemistry*; D006031:Glycosylation; D006868:Hydrolysis; D007529:Isoflavones / Q000737:chemistry*; D041942:Soy Milk / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism*; D013997:Time Factors; D001617:beta-Glucosidase / Q000378:metabolism
D004967:Estrogens; D006027:Glycosides; D007529:Isoflavones; C040641:genistin; C013908:daidzin; D001617:beta-Glucosidase