[An in vitro investigation of the regulation mechanism to the blood-labyrinth barrier permeability of guinea pig inner ear].
OBJECTIVE
To investigate the regulation mechanism of the blood-labyrinth barrier permeability of guinea pig inner ear.


METHODS
The cultured endothelial cell was derived from the stria vascularis from guinea pig inner ear. We exposed guinea pig inner ear capillary endothelial cell monolayers to substances known to increase or decrease cyclic AMP and measured their effect on endothelial permeability to albumin and Evan's blue and endothelial cell cyclic AMP and F-actin concentrations.


RESULTS
Cholera toxin (CT) led to a remarkable increase in cyclic AMP concentration and a marked reduction in F-actin concentration which was associated with remarkable reduction in albumin and Evan's blue transfer across endothelial monolayers; In contrast, katlex led to an obvious reduction in cyclic AMP concentration and a marked increase in F-actin concentration which was associated with obvious increase in albumin and Evan's blue transfer across endothelial monolayers.


CONCLUSIONS
The results indicated that cyclic AMP is an important determinant of the blood-labyrinth barrier in vitro. The barrier function changes are probably associated with apparent endothelial shape changes and qualitative and quantitative changes in F-actin.
Pan
Mingjin
M
Department of Otolaryngology, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China. pmj68923@sohu.com
Zhang
Xueyuan
X
D000199:Actins / Q000378:metabolism; D000818:Animals; D002199:Capillary Permeability; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D007758:Ear, Inner / Q000098:blood supply; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006168:Guinea Pigs; D055806:Microvessels / Q000166:cytology / Q000378:metabolism*
D000199:Actins; D000242:Cyclic AMP