title: [Effect of HSP72 on acute injury of neonatal cardiomyocytes induced by oxidative stress].
abstract: OBJECTIVE
  To explore the protective effect of HSP72 on the acute injury of cardiomyocyte induced by oxidative stress.
  
  
  METHODS
  Cardiomyocytes of neonatal rats treated with heat shock (42 degrees C, 30 min, recovery for 6 h) to induce the expression of HSP72 and HSP72 antisense oligonucleotide was transformed to block the expression of HSP72. 0.5 mmol/L (final concentration) H2O2 was added into the culture medium to mimic oxidative stress, and to induce the acute injury of neonatal cardiomyocytes. The release of LDH and the total protein synthesis were applied to evaluate the injury of cardiomyocyte of neonatal rats.
  
  
  RESULTS
  Oxidative stress could significantly increase the release of LDH, and inhibit the total protein synthesis. By inducing the expression of HSPs, heat shock pretreatment significantly reduced the release of LDH and relieved the oxidative stress-mediated inhibition of total protein synthesis. Moreover, HSP7-2 anti-sense oligonucleotide could remarkably block the protective effect of heat shock pretreatment on the cellular injuries induced by H2O2.
  
  
  CONCLUSIONS
  HSP72 plays a most important role in the acute injury of cardiomyocyte mediated by oxidative stress.
authors.affiliation: Cell Biology Research Laboratory, Xiangya School of Medicine, Central South University, Changsha, China. luoxuebing@126.com
authors.forename: Xue-bing
authors.initials: XB
authors.lastname: Luo
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Yi
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Mei-dong
authors.initials: MD
authors.lastname: Liu
authors.forename: Xian-zhong
authors.initials: XZ
authors.lastname: Xiao
authors.forename: Gong-hua
authors.initials: GH
authors.lastname: Deng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D050884:HSP72 Heat-Shock Proteins / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000473:pathology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D018384:Oxidative Stress; D014176:Protein Biosynthesis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D050884:HSP72 Heat-Shock Proteins; D016376:Oligonucleotides, Antisense; D007770:L-Lactate Dehydrogenase