title: A rapid and efficient method for DNA extraction from paraffin wax embedded tissue for PCR amplification.
abstract: DNA from archival, formaldehyde fixed, paraffin wax embedded human tissue, suitable for amplification by the polymerase chain reaction (PCR), was obtained using a microwave method based on the capture of DNA by magnetic beads. Fragments of the alpha-1-antitrypsin gene (AAT) and the apolipoprotein E gene (APOE) were amplified successfully from human liver and brain tissue, respectively. This procedure provides a more rapid, simple and efficient method for reproducibly obtaining DNA from preserved tissue that has been kept in storage for up to 30 years.
authors.affiliation: Division of Clinical Chemistry, Department of Clinical Laboratory Sciences, Queen's Medical Centre, Nottingham NG7 2UH.
authors.forename: K
authors.initials: K
authors.lastname: Morgan
authors.forename: L
authors.initials: L
authors.lastname: Lam
authors.forename: N
authors.initials: N
authors.lastname: Kalsheker