title: A B-lectin receptor kinase gene conferring rice blast resistance.
abstract: Rice blast, caused by the fungal pathogen Magnaporthe grisea, is one of the most devastating diseases in rice worldwide. The dominant resistance gene, Pi-d2 [previously named Pi-d(t)2], present in the rice variety Digu, confers gene-for-gene resistance to the Chinese blast strain, ZB15. Pi-d2 was previously mapped close to the centromere of chromosome 6. In this study, the Pi-d2 gene was isolated by a map-based cloning strategy. Pi-d2 encodes a receptor-like kinase protein with a predicted extracellular domain of a bulb-type mannose specific binding lectin (B-lectin) and an intracellular serine-threonine kinase domain. Pi-d2 is a single-copy gene that is constitutively expressed in the rice variety Digu. Transgenic plants carrying the Pi-d2 transgene confer race-specific resistance to the M. grisea strain, ZB15. The Pi-d2 protein is plasma membrane localized. A single amino acid difference at position 441 of Pi-d2 distinguishes resistant and susceptible alleles of rice blast resistance gene Pi-d2. Because of its novel extracellular domain, Pi-d2 represents a new class of plant resistance genes.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Chen
authors.forename: Junjun
authors.initials: J
authors.lastname: Shang
authors.forename: Dexi
authors.initials: D
authors.lastname: Chen
authors.forename: Cailin
authors.initials: C
authors.lastname: Lei
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.forename: Wenxue
authors.initials: W
authors.lastname: Zhai
authors.forename: Guozhen
authors.initials: G
authors.lastname: Liu
authors.forename: Jichen
authors.initials: J
authors.lastname: Xu
authors.forename: Zhongzhuan
authors.initials: Z
authors.lastname: Ling
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.forename: Bingtian
authors.initials: B
authors.lastname: Ma
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wang
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zhao
authors.forename: Shigui
authors.initials: S
authors.lastname: Li
authors.forename: Lihuang
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D018628:Gene Dosage; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000032:analysis; D007113:Immunity, Innate / Q000235:genetics; D020082:Magnaporthe / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000032:analysis; D017422:Sequence Analysis, DNA
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D017346:Protein Serine-Threonine Kinases