title: Palmitoylation of huntingtin by HIP14 is essential for its trafficking and function.
abstract: Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Here we show that huntingtin (htt) is normally palmitoylated at cysteine 214, which is essential for its trafficking and function. The palmitoylation and distribution of htt are regulated by the palmitoyl transferase huntingtin interacting protein 14 (HIP14). Expansion of the polyglutamine tract of htt, which causes Huntington disease, results in reduced interaction between mutant htt and HIP14 and consequently in a marked reduction in palmitoylation. Mutation of the palmitoylation site of htt, making it palmitoylation resistant, accelerates inclusion formation and increases neuronal toxicity. Downregulation of HIP14 in mouse neurons expressing wild-type and mutant htt increases inclusion formation, whereas overexpression of HIP14 substantially reduces inclusions. These results suggest that the expansion of the polyglutamine tract in htt results in decreased palmitoylation, which contributes to the formation of inclusion bodies and enhanced neuronal toxicity.
authors.affiliation: Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada.
authors.forename: Anat
authors.initials: A
authors.lastname: Yanai
authors.forename: Kun
authors.initials: K
authors.lastname: Huang
authors.forename: Rujun
authors.initials: R
authors.lastname: Kang
authors.forename: Roshni R
authors.initials: RR
authors.lastname: Singaraja
authors.forename: Pamela
authors.initials: P
authors.lastname: Arstikaitis
authors.forename: Lu
authors.initials: L
authors.lastname: Gan
authors.forename: Paul C
authors.initials: PC
authors.lastname: Orban
authors.forename: Asher
authors.initials: A
authors.lastname: Mullard
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Cowan
authors.forename: Lynn A
authors.initials: LA
authors.lastname: Raymond
authors.forename: Renaldo C
authors.initials: RC
authors.lastname: Drisdel
authors.forename: William N
authors.initials: WN
authors.lastname: Green
authors.forename: Brinda
authors.initials: B
authors.lastname: Ravikumar
authors.forename: David C
authors.initials: DC
authors.lastname: Rubinsztein
authors.forename: Alaa
authors.initials: A
authors.lastname: El-Husseini
authors.forename: Michael R
authors.initials: MR
authors.lastname: Hayden
mesh_terms: D000217:Acyltransferases; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003545:Cysteine / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D006801:Humans; D000071058:Huntingtin Protein; D002479:Inclusion Bodies / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019308:Palmitic Acid / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D019680:Trinucleotide Repeat Expansion / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C506812:Htt protein, mouse; D000071058:Huntingtin Protein; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010455:Peptides; C097188:polyglutamine; D019308:Palmitic Acid; D000217:Acyltransferases; C473038:ZDHHC17 protein, human; D003545:Cysteine