title: Characterization of monoclonal antibodies directed against the canine distemper virus nucleocapsid protein.
abstract: We have established four monoclonal antibodies (MAbs) against the nucleocapsid protein (NP) of canine distemper virus (CDV). A competitive binding assay has revealed that the MAbs are directed against two antigenic domains. An immunofluorescence assay using a series of deletion clones of the NP and an immunoprecipitation assay using the NP have revealed that two of the MAbs recognize the C-terminal region of the NP while the other two recognize the tertiary structure of the N-terminal domain. These MAbs reacted with all eight strains of CDV used in this study, but showed different reactivities against measles virus and rinderpest virus.
authors.affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo 108-8641, Japan.
authors.forename: Munemitsu
authors.initials: M
authors.lastname: Masuda
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamata
authors.forename: Tomoe
authors.initials: T
authors.lastname: Katsuo
authors.forename: Akiko
authors.initials: A
authors.lastname: Takenaka
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Miura
authors.forename: Misako
authors.initials: M
authors.lastname: Yoneda
authors.forename: Kyoko
authors.initials: K
authors.lastname: Tsukiyama-Kohara
authors.forename: Kiyohisa
authors.initials: K
authors.lastname: Mizumoto
authors.forename: Chieko
authors.initials: C
authors.lastname: Kai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D000918:Antibody Specificity; D000940:Antigenic Variation; D001667:Binding, Competitive; D019556:COS Cells; D002522:Chlorocebus aethiops; D004216:Distemper / Q000821:virology; D004217:Distemper Virus, Canine / Q000276:immunology*; D004285:Dogs; D018604:Epitope Mapping; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D008807:Mice, Inbred BALB C; D016254:Mutagenesis, Insertional; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D015531:Radioimmunoprecipitation Assay; D014709:Vero Cells
chemical_list: D000911:Antibodies, Monoclonal; D019590:Nucleocapsid Proteins; D012367:RNA, Viral