[Identification of aging related proteins in human normal colonic epithelium].
OBJECTIVE
To explore the molecular mechanisms of colonic epithelial aging related proteins and aged colonic epithelial susceptibility to tumor.


METHODS
The proteins of normal human colonic epithelial tissue from young and old people were separated by 2-dimensional gel electrophoresis (2DGE), respectively. Then gels were stained by silver, scanned by imagescanner and analyzed with PDQuest software. The differentially expressed protein spots of colonic epithelium between the old and the young groups were identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and database searching.


RESULTS
Well-resolved and reproducible 2DGE maps of normal human colonic epithelium from the young and the old were acquired. Nineteen more than 2 fold differentially expressed protein spots were identified representing 17 different proteins by MALDI-TOF-MS. The functions of these proteins involve in metabolism, energy generation, transportation, antioxidation, translation and protein folding.


CONCLUSIONS
Seventeen aging related proteins of human colonic epithelium identified indicate that injury of mitochondrial function and decline of antioxidant capability are important reasons for the aging of human colonic epithelium. These data provided useful clues for elucidating the mechanisms of colonic epithelial aging and aged colonic epithelial susceptibility to cancer.
Zhu
Guo
G
Key Laboratory of Cancer Proteomics of Chinese Ministry of Public Health, Xiangya Hospital, Central South University, Changsha.
Xiao
Zhi-qiang
ZQ
Chen
Zhu-chu
ZC
Li
Jian-ling
JL
Zhang
Peng-fei
PF
Yang
Yi-xuan
YX
Feng
Xue-ping
XP
Yuan
Wei-jian
WJ
D000375:Aging / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics*; D018118:Chloride Channels / Q000096:biosynthesis* / Q000235:genetics; D003106:Colon / Q000166:cytology*; D044927:Electron-Transferring Flavoproteins / Q000096:biosynthesis* / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology; D011506:Proteins / Q000378:metabolism
C501966:CLIC1 protein, human; D018118:Chloride Channels; C500318:ETFA protein, human; D044927:Electron-Transferring Flavoproteins; D011506:Proteins