title: Morphine modulates monocyte-macrophage conversion phase.
abstract: Monocyte migration and their activation into the macrophage phenotype play a role in the modulation of tissue injury. We studied the effect of morphine on the monocyte-macrophage conversion phase (MMCP). Phorbol 12-myristate 13-acetate (PMA) activated THP-1 cells and promoted their adhesion to the substrate. Morphine inhibited PMA-induced MMCP. However, opiate receptor antagonists attenuated this effect of morphine. Interestingly, PMA as well as morphine-stimulated superoxide production by monocytes. Superoxide dismutase (SOD) not only inhibited PMA-mediated MMCP but also attenuated the inhibitory effect of morphine. PMA not only enhanced adhesion of monocytes to a filter but also promoted their migration. These findings suggest that the PMA-induced macrophage phenotype conversion may be accelerating their migration; whereas, morphine may be preventing the migration of monocytes by inhibiting MMCP.
authors.affiliation: Long Island Jewish Medical Center, New Hyde Park, NY 11040, USA.
authors.forename: Ikuske
authors.initials: I
authors.lastname: Hatsukari
authors.forename: Naoko
authors.initials: N
authors.lastname: Hitosugi
authors.forename: Amit
authors.initials: A
authors.lastname: Dinda
authors.forename: Pravin C
authors.initials: PC
authors.lastname: Singhal
mesh_terms: D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement; D002478:Cells, Cultured; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects*; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D009020:Morphine / Q000494:pharmacology*; D009292:Narcotic Antagonists; D011957:Receptors, Opioid / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D009292:Narcotic Antagonists; D011957:Receptors, Opioid; D013481:Superoxides; D009020:Morphine; D013755:Tetradecanoylphorbol Acetate