title: Persistence of betanodavirus in Barramundi brain (BB) cell line involves the induction of Interferon response.
abstract: The BB cell line derived from the brain tissue of a barramundi (Lates calcarifer) that survived nervous necrosis virus (NNV) infection is persistently infected with NNV. To elucidate whether interferon (IFN) plays a role in the mechanism of NNV-persistent infection in BB cell line, a virus-negative control cell line was obtained by treating BB cells with NNV-specific rabbit antiserum for 5 subcultures. After the treatment, NNV titer or RNA or capsid protein was no longer detected in the cured BB (cBB) cells. Expression of Mx gene, encoding a type I IFN-inducible antiviral protein, was found in BB cells and cBB cells following NNV infection, but not in NNV-free cBB cells. Moreover, expression of Mx gene and antiviral activity against NNV were induced in cBB cells by the treatment with MAb-neutralized BB cell supernatant. Furthermore, NNV persistent infection was induced again in cBB cell culture if multiplicity of infection (MOI) was low (< or = 1). These experimental results indicated that IFN-like cytokines existed in the culture supernatant of BB cells, and IFN-induced response played an important role in protecting the majority of cells from virus lytic infection and regulating NNV persistence in the BB cell line.
authors.affiliation: Institute of Zoology and Department of Life Science, National Taiwan University, 1, Sec, 4, Roosevelt Rd., Taipei 10617, Taiwan, ROC.
authors.forename: Y C
authors.initials: YC
authors.lastname: Wu
authors.forename: S C
authors.initials: SC
authors.lastname: Chi
mesh_terms: D000199:Actins / Q000032:analysis / Q000096:biosynthesis; D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000276:immunology / Q000821:virology*; D002460:Cell Line; D017931:DNA Primers / Q000737:chemistry; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D007372:Interferons / Q000276:immunology; D064547:Myxovirus Resistance Proteins; D030381:Nodaviridae / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010473:Perciformes; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D000199:Actins; D000914:Antibodies, Viral; D017931:DNA Primers; D064547:Myxovirus Resistance Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D007372:Interferons; D019204:GTP-Binding Proteins