title: Capturing the uncultivated majority.
abstract: The metagenomic analysis of environmental microbial communities continues to be a rapidly developing area of study. DNA isolation, the first step in capturing the uncultivated majority, has seen many advances in recent years. Protocols have been developed to distinguish DNA from live versus dead cells and to separate extracellular from intracellular DNA. Looking to increase our understanding of the role that members of a microbial community play in ecological processes, several techniques have been developed that are enabling greater in-depth analysis of environmental metagenomes. These include the development of environmental gene tags and the serial analysis of 16S rRNA gene sequence tags. In addition, new screening methods have been designed to select for specific functional genes within metagenomic libraries. Finally, new cultivation methods continue to be developed to improve our ability to capture a greater diversity of microorganisms within the environment.
authors.affiliation: Diversa Corporation, San Diego, CA 92121, USA.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Green
authors.forename: Martin
authors.initials: M
authors.lastname: Keller
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D004783:Environmental Microbiology; D015870:Gene Expression; D015723:Gene Library; D005827:Genetics, Microbial / Q000379:methods*; D016680:Genome, Bacterial; D016681:Genome, Fungal; D008828:Microbiological Techniques / Q000379:methods*; D010802:Phylogeny
chemical_list: D004247:DNA