Comparative analysis of three indigenous plasmids from Xanthomonas axonopodis pv. glycines.
The complete nucleotide sequences of three representative plasmids, pAG1 from Xanthomonas axonopodis pv. glycines strain AG1, and pXAG81 and pXAG82 from strain 8ra, were determined. The sizes of pAG1, pXAG81, and pXAG82 are 15143, 26721, and 1315 base pairs, respectively. A possible 16, 34, and 1 open-reading frames (ORFs) are present in pAG1, pXAG81, and pXAG82, respectively. pAG1 could encode proteins homologous to AvrBs3, TnpA, TnpR, RepA, HtrA, ParA, M.XmaI, R.XmaI, and six hypothetical proteins. pXAG81 possibly encodes proteins homologous to those involved in conjugal plasmid transfer. Possible oriT sequences similar to those of RP4 were found between mobB and mobC homologs. At the end of the RepA homolog in pAG1 and pXAG81, a putative oriV region at the 3'-end of RepA similar to the integron TNCP23 in pKLC102 of Pseudomonas aeruginosa C strain was found. All 255 isolates carried either pAG1 type or pXAG81 type, and 217 isolates appeared to carry tra gene homologs. Both pAG1 and pXAG81 types contained an avrBs3 homolog varying from three copies in AG1 to eight copies in AG166.
Kim
Jung-Gun
JG
School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921, Republic of Korea.
Choi
Sejung
S
Oh
Jonghee
J
Moon
Jae Sun
JS
Hwang
Ingyu
I
D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity; D014974:Xanthomonas / Q000235:genetics* / Q000472:pathogenicity