title: Novel immunoblotting monoclonal antibodies against human and rat CD36/fat used to identify an isoform of CD36 in rat muscle.
abstract: CD36, a surface membrane glycoprotein, functions as a class B scavenger receptor that binds to several distinct ligands. Included among these is oxidized low-density lipoprotein (Ox-LDL), a major trigger of atherosclerotic lesions, and the levels of CD36 activity and Ox-LDL uptake may have an impact on coronary artery disease. In addition, recent studies in rodents have shown that CD36, also known as FAT, controls the levels of free fatty acids and triglycerides in plasma, and is an important regulator of the metabolic pathways involved in insulin resistance. Despite the importance of measuring CD36 expression in different tissues there is a paucity of good immunoblotting antibodies, particularly for rodent tissue. Here, using GST-fusion proteins incorporating the cysteine cluster encoded by exons VIII, IX, and X of the CD36 gene as immunogen, we have generated a panel of monoclonal antibodies that are excellent blotting reagents for human and rat CD36. With these reagents we were able to visualize an additional, faster migrating CD36 band in rat muscle, likely representing a minor splice variant of CD36 (CD36var.1) hitherto seen only in the human HEL cell line.
authors.affiliation: Cancer Research Unit, Faculty of Health, The University of Newcastle, Callaghan, and The Hunter Medical Research Institute, NSW, Australia.
authors.forename: Rick F
authors.initials: RF
authors.lastname: Thorne
authors.forename: Xinhai
authors.initials: X
authors.lastname: Zhang
authors.forename: Chaojun
authors.initials: C
authors.lastname: Song
authors.forename: Boquan
authors.initials: B
authors.lastname: Jin
authors.forename: Gordon F
authors.initials: GF
authors.lastname: Burns
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods*; D018955:CD36 Antigens / Q000276:immunology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009132:Muscles / Q000276:immunology*; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D000911:Antibodies, Monoclonal; D018955:CD36 Antigens