title: [Effect of simvastatin on the proliferation of human omental preadipocytes and caspase-3 expression].
abstract: OBJECTIVE
  To explore the effect of simvastatin on the cell cycle and caspase-3 expression in human omental preadipocytes.
  
  
  METHODS
  The preadipocytes were randomly divided into a blank group, a 10(-5) mol/L simvastatin group, and a 10(-4) mol/L simvastatin group. Each group was incubated with different concentrations of simvastatin for 48 hours. MTT method was used to analyze the effect of simvastatin on the proliferation. Distribution of the cell cycle was measured by flow cytometric. Caspase-3 expression was examined by cyto- immunochemistry.
  
  
  RESULTS
  After being induced to differentiate for 16 days the human omental preadipocytes developed to mature adipocyte penotypes with lipid droplet. Simvastatin 10(-4) mol/L had significant anti-proliferation effect. Flow cytometric analysis showed the cell cycle was blocked in G0/G1 phase and caspase-3 positive cells increased dramatically.
  
  
  CONCLUSIONS
  Simvastatin may block the cells in G0/G1 phase, and induce caspase-3 expression, which may trigger apoptosis.
authors.affiliation: Department of Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ping-fang
authors.initials: PF
authors.lastname: Wang
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Sheng
authors.forename: Jian-zheng
authors.initials: JZ
authors.lastname: Fang
authors.forename: Feng-ying
authors.initials: FY
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects*; D000924:Anticholesteremic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis* / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D009852:Omentum / Q000166:cytology; D019821:Simvastatin / Q000494:pharmacology*
chemical_list: D000924:Anticholesteremic Agents; D019821:Simvastatin; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases