title: Subdoligranulum variabile gen. nov., sp. nov. from human feces.
abstract: During studies on the microflora of human feces we have isolated a strictly anaerobic, non-spore-forming, Gram-negative staining organism which exhibits a somewhat variable coccus-shaped morphology. Comparative 16S ribosomal RNA gene sequencing studies show the unidentified organism is phylogenetically a member of the Clostridium leptum supra-generic rRNA cluster and displays a close affinity to some rDNA clones derived from human and pig feces. The nearest named relatives of the unidentified isolate corresponded to Faecalibacterium prausnitzii (formerly Fusobacterium prausnitzii) displaying a 16S rRNA sequence divergence of approximately 9%, with Anaerofilum agile and A. pentosovorans the next closest relatives of the unidentified bacterium (sequence divergence approximately 10%). Based on phenotypic and phylogenetic considerations, it is proposed that the unusual coccoid-shaped organism be classified as a new genus and species, Subdoligranulum variabile. The type strain of S. variabile is BI 114(T) (=CCUG 47106(T)=DSM 15176(T)).
authors.affiliation: Bioneer A/S, Department of Molecular Characterization, Kogle Alle 2, DK-2970 Hørsholm, Denmark. kho@bioneer.dk
authors.forename: Kim
authors.initials: K
authors.lastname: Holmstrøm
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Collins
authors.forename: Trine
authors.initials: T
authors.lastname: Møller
authors.forename: Enevold
authors.initials: E
authors.lastname: Falsen
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lawson