title: Analysis of asbestos-induced gene expression changes in bronchiolar epithelial cells using laser capture microdissection and quantitative reverse transcriptase-polymerase chain reaction.
abstract: Laser capture microdissection (LCM) enables the removal of discrete microstructures or cell types from properly prepared histological sections. Extraction of RNA from microdissected tissue followed by quantitative reverse transcriptase-polymerase chain (QRT-PCR) reaction permits the analysis of cell-type or microstructure-specific gene expression changes that occur in response to various stimuli in the environment. In our lab, the combination of LCM and QRT-PCR has proven very useful in the determination of the in vivo gene expression changes that occur in bronchiolar epithelium in response to inhalation of crocidolite asbestos. A detailed description of the preparation of cDNA from bronchiolar epithelial cells obtained by LCM is described in this work.
authors.affiliation: Department of Pathology, University of Vermont, Burlington, VT, USA.
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Manning
authors.forename: Brooke T
authors.initials: BT
authors.lastname: Mossman
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Taatjes
mesh_terms: D017638:Asbestos, Crocidolite / Q000494:pharmacology*; D001980:Bronchi / Q000166:cytology* / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000295:instrumentation / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods; D012313:RNA / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods
chemical_list: D017638:Asbestos, Crocidolite; D012313:RNA