Tubulin is the target binding site for NAP-related peptides: ADNF-9, D-NAP, and D-SAL.
The authors set out to investigate whether NAP-related peptides interact with tubulin at a NAP binding site. Previous studies have shown that the neuroprotective peptide NAP binds to tubulin. As NAP (NAPVSIPQ) shares structural similarities with ADNF-9 (SALLRSIPA), and the all-D-enantiomers, D-NAP and D-SAL, it was hypothesized that all of these peptides compete with NAP-tubulin binding. Using NAP affinity column and extracts from newborn rat brain (cerebral cortex), we now show that the above-mentioned peptides compete with NAP binding to tubulin. The identification of tubulin as a target binding site for NAP-related peptides explains, in part, the broad neuroprotective activity offered by these potent peptides.
Holtser-Cochav
Miri
M
Human Molecular Genetics & Biochemistry, Sackler Medical School, Tel Aviv University, Israel.
Divinski
Inna
I
Gozes
Illana
I
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001665:Binding Sites; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D008969:Molecular Sequence Data; D018696:Neuroprotective Agents / Q000378:metabolism; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism*
D018696:Neuroprotective Agents; D009842:Oligopeptides; D010455:Peptides; D014404:Tubulin; C425904:davunetide