The economics of inclusion body processing.
Many recombinant proteins are often over-expressed in host cells, such as Escherichia coli, and are found as insoluble and inactive protein aggregates known as inclusion bodies (IBs). Recently, a novel process for IB extraction and solubilisation, based on chemical extraction, has been reported. While this method has the potential to radically intensify traditional IB processing, the process economics of the new technique have yet to be reported. This study focuses on the evaluation of process economics for several IB processing schemes based on chemical extraction and/or traditional techniques. Simulations and economic analysis were conducted at various processing conditions using granulocyte macrophage-colony stimulating factor, expressed as IBs in E. coli, as a model protein. In most cases, IB processing schemes based on chemical extraction having a shorter downstream cascade demonstrated a competitive economic edge over the conventional route, validating the new process as an economically more viable alternative for IB processing.
Lee
G H
GH
Department of Chemical and Biomolecular Engineering, National University of Singapore, 10 Kent Ridge Crescent, 117576, Singapore, Singapore.
Cooney
D
D
Middelberg
A P J
AP
Choe
W S
WS
D019149:Bioreactors / Q000191:economics*; D003365:Costs and Cost Analysis; D004926:Escherichia coli / Q000254:growth & development; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000191:economics* / Q000302:isolation & purification; D006801:Humans; D002479:Inclusion Bodies; D011994:Recombinant Proteins / Q000191:economics / Q000302:isolation & purification
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor