[Influence of biomateriai on focal adhesion kinase of platelet: ex vivo study].
This study was aimed to detect the biomaterial-induced phosphorylation level of platelet focal adhesion kinase, a key component of signal transduction pathways. Flat tubings, made from 5 biomaterials: organotin-PVC, PU50, PU60, PU70, and PTFE,were connected to an ex vivo flow circuit with canine femoral artery and vein. After 5 min, the adherent platelets on the inner surface of the tubing were lysed, then FAK and phospho-FAK were examined, using immunoprecipitation and Western blot. The results showed a significant increase in the FAK value on the PVC and PTFE surface,compared to the PUs; among the PUs, the FAK value decreased in the following order: PU70/PU60/PU50. The phospho-FAK value on the five groups of surface decreased in the following order: PVC/PTFE/PU70, PU60, PU50; there was no significant difference among the PUs with regard to the phospho-FAK value. In conclusion, after platelet-material interaction, biomateriais may lead to the phosphorylation of FAK of platelets;the level of phosphorylation of FAK has been positively linked to the thrombogenicity of materials; the phospho-FAK value may be a valid molecular parameter of the haemocompatibility assessment; the FAK value may supply references to the haemocompatibility assessment.
Wei
Wenjia
W
The Ninth People's Hospital, Shanghai Second Medical University, Shanghai Biomaterials Research & Testing Center, Shanghai 200023, China.
Sun
Jiao
J
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001792:Blood Platelets / Q000201:enzymology*; D004285:Dogs; D005263:Femoral Artery / Q000601:surgery; D005268:Femoral Vein / Q000601:surgery; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000737:chemistry*; D008422:Materials Testing; D010766:Phosphorylation
D001672:Biocompatible Materials; D051416:Focal Adhesion Protein-Tyrosine Kinases