title: PUF proteins bind Pop2p to regulate messenger RNAs.
abstract: PUF proteins, a family of RNA-binding proteins, interact with the 3' untranslated regions (UTRs) of specific mRNAs to control their translation and stability. PUF protein action is commonly correlated with removal of the poly(A) tail of target mRNAs. Here, we focus on how PUF proteins enhance deadenylation and mRNA decay. We show that a yeast PUF protein physically binds Pop2p, which is a component of the Ccr4p-Pop2p-Not deadenylase complex, and that Pop2p is required for PUF repression activity. By binding Pop2p, the PUF protein simultaneously recruits the Ccr4p deadenylase and two other enzymes involved in mRNA regulation, Dcp1p and Dhh1p. We reconstitute regulated deadenylation in vitro and demonstrate that the PUF-Pop2p interaction is conserved in yeast, worms and humans. We suggest that the PUF-Pop2p interaction underlies regulated deadenylation, mRNA decay and repression by PUF proteins.
authors.affiliation: Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, Wisconsin 53706, USA.
authors.forename: Aaron C
authors.initials: AC
authors.lastname: Goldstrohm
authors.forename: Brad A
authors.initials: BA
authors.lastname: Hook
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Seay
authors.forename: Marvin
authors.initials: M
authors.lastname: Wickens
mesh_terms: D020413:3' Untranslated Regions; D000249:Adenosine Monophosphate / Q000378:metabolism; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000502:physiology; D017931:DNA Primers; D019143:Evolution, Molecular; D006868:Hydrolysis; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000502:physiology; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: D020413:3' Untranslated Regions; D018797:Cell Cycle Proteins; D017931:DNA Primers; C449502:MPT5 protein, S cerevisiae; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D000249:Adenosine Monophosphate; D012260:Ribonucleases; C079009:POP2 protein, S cerevisiae