title: Proteomic comparison of prostate cancer cell lines LNCaP-FGC and LNCaP-r reveals heatshock protein 60 as a marker for prostate malignancy.
abstract: BACKGROUND
  Androgen-sensitive prostate cancer cell-line LNCaP-FGC and androgen-resistant line LNCaP-r constitute a model for development of androgen resistance in prostate cancer.
  
  
  METHODS
  Proteins differently expressed in the two cell-lines were identified by two-dimensional (2-D) electrophoresis and mass spectrometry. HSP60, more abundant in LNCaP-r, was studied by RT-PCR and immunohistochemistry in specimens of human prostate cancer.
  
  
  RESULTS
  HSP60 was upregulated in LNCaP-r, nm23 in LNCaP-FGC, and titin (two isoforms) in either LNCaP-r or LNCaP-FGC. In non-malignant prostate, HSP60-staining was in the glandular compartment, particularly basal epithelial cells. In prostate cancer, most epithelial cells showed moderate-strong staining without apparent correlation between staining intensity and Gleason grade.
  
  
  CONCLUSIONS
  The LNCaP-FGC/LNCaP-r model, characterized by 2-D electrophoresis, reveals distinct proteomic alterations. With HSP60, results from cell-lines correlated with clinical results, indicating that this model can be used for dissection of mechanisms involved in transformation to androgen resistance and assignment of protein markers in prostate cancer.
authors.affiliation: Department of Clinical Neuroscience, Karolinska Hospital, Karolinska Institutet, Stockholm, Sweden. Bjorn.Johansson@neuro.ki.se
authors.forename: Björn
authors.initials: B
authors.lastname: Johansson
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Pourian
authors.forename: Yin-Choy
authors.initials: YC
authors.lastname: Chuan
authors.forename: Irene
authors.initials: I
authors.lastname: Byman
authors.forename: Anders
authors.initials: A
authors.lastname: Bergh
authors.forename: See-Tong
authors.initials: ST
authors.lastname: Pang
authors.forename: Gunnar
authors.initials: G
authors.lastname: Norstedt
authors.forename: Tomas
authors.initials: T
authors.lastname: Bergman
authors.forename: Ake
authors.initials: A
authors.lastname: Pousette
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D013058:Mass Spectrometry; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D018834:Chaperonin 60