[Interaction of laminin with the plasma membrane components of ascitic Zajdela hepatoma cells].
The laminin affinity chromatography was used for isolating laminin-binding proteins from the plasma membrane of Zajdela hepatoma cells synthesizing laminin. These were components with mol. weights about 80, 67, 60, 55, 52, 48 and 43 kDa. The isolation of laminin integrin receptors from plasma membranes of Zajdela hepatoma cells in the presence of MnCl2 detected only a protein with mol. weight about 80 kDa in EDTA-elution conditions. This protein was identified by mass spectrometry method as the 78 kDa glucose-regulated protein precursor (GRP78). It belongs to the family of 70 kDa heat shock proteins, recently GRP78 was reported to be localized on the surface of different cell types, including hepatocytes.
Tiuriaeva
I I
II
Mirgorodskaia
O A
OA
Cherepanova
O A
OA
Podol'skaia
E P
EP
Serebriakova
M V
MV
Ivanov
V A
VA
D000595:Amino Acid Sequence; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002847:Chromatography, Agarose; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007797:Laminin / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D018832:Molecular Chaperones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism
D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D007797:Laminin; D018832:Molecular Chaperones; D011506:Proteins; D011956:Receptors, Cell Surface