Retention modelling in ternary solvent-strength gradient elution reversed-phase chromatography using 30 mm columns.
An optimization strategy for ternary solvent-strength gradient elution RP chromatography is described in which a two-dimensional model of gradient time (2 levels) against ternary proportions of organic modifiers (4 levels) was constructed. From the resolution surface the optimum ratio of organic modifiers could be selected. Excellent retention time and acceptable peak width and resolution simulations were obtained. The separation could be further optimized from the same input data by using a standard one-dimensional model in order to optimize for gradient slope, duration and shape. Excellent retention time and acceptable peak width and resolution simulations were obtained (< 1, 2 and 6% error, respectively).
Euerby
Melvin R
MR
AstraZeneca R&D Charnwood, Analytical Development, Pharmaceutical and Analytical R&D - Charnwood/Lund, Bakewell Road, Loughborough, Leicestershire LE11 5RH, UK. mel.euerby@astrazeneca.com
Scannapieco
Federico
F
Rieger
Hans-Jürgen
HJ
Molnar
Imre
I
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D003198:Computer Simulation; D012984:Software; D012997:Solvents / Q000737:chemistry*
D012997:Solvents