[Comparison study of hair cell markers in chicken inner ear].
OBJECTIVE
To supply valuable markers for hair cell development and regeneration.


METHODS
Cryosections of new hatched chicken inner ear were made. The position and intensity of Hair Cell Antigen, Espin, and Myosin7a expression were detected by indirect immunocytochemistry.


RESULTS
Hair Cell Antigen, Espin, and Myosin7a were all expressed in both auditory and vestibular hair cell. Hair Cell Antigen was located in the apical part of hair cell and hair cell bundles. Espin was expressed in the hair cell bundles and cuticular plate. Myosin7a immu no-staining resulted in the typical cytoplasm staining.


CONCLUSIONS
Hair Cell Antigen, Espin, and Myosin7a are ideal markers for hair cell and can be used for studying the hair cell development and regeneration. These markers combined with F-actin staining could give a better result for qualitative and quantitative investigation for hair cell development and regeneration.
Wang
Yucheng
Y
Department of Otoneurology and Skull Base Surgery, Eye and ENT Hospital of Fudan University, Shanghai, 200031, China.
Wang
Zhengmin
Z
Shen
Yunzhen
Y
Li
Wen
W
Li
Huawei
H
D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D002645:Chickens; D007758:Ear, Inner / Q000378:metabolism*; D006198:Hair Cells, Auditory / Q000378:metabolism*
D015415:Biomarkers