title: Zebrafish Trap230/Med12 is required as a coactivator for Sox9-dependent neural crest, cartilage and ear development.
abstract: The vertebrate Sox9 transcription factor directs the development of neural crest, otic placodes, cartilage and bone. In zebrafish, there are two Sox9 orthologs, Sox9a and Sox9b, which together perform the functions of the single-copy tetrapod Sox9. In a large-scale genetic screen, we have identified a novel zebrafish mutant that strongly resembles the Sox9a/Sox9b double mutant phenotype. We show that this mutation disrupts the zebrafish Trap230/Med12 ortholog, a member of the Mediator complex. Mediator is a coactivator complex transducing the interaction of DNA-binding transcription factors with RNA polymerase II, and our results reveal a critical function of the Trap230 subunit as a coactivator for Sox9.
authors.affiliation: European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors.forename: Marlene J
authors.initials: MJ
authors.lastname: Rau
authors.forename: Sabine
authors.initials: S
authors.lastname: Fischer
authors.forename: Carl J
authors.initials: CJ
authors.lastname: Neumann
mesh_terms: D000818:Animals; D002356:Cartilage / Q000196:embryology*; D004423:Ear / Q000196:embryology*; D024002:HMGB Proteins / Q000502:physiology*; D006609:High Mobility Group Proteins / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000502:physiology*; D056892:Mediator Complex; D009432:Neural Crest / Q000196:embryology*; D010641:Phenotype; D021122:Protein Subunits / Q000235:genetics / Q000502:physiology; D011988:Receptors, Thyroid Hormone / Q000235:genetics / Q000502:physiology*; D055758:SOX9 Transcription Factor; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D024002:HMGB Proteins; D006609:High Mobility Group Proteins; D047908:Intracellular Signaling Peptides and Proteins; C513481:Med12 protein, zebrafish; D056892:Mediator Complex; D021122:Protein Subunits; D011988:Receptors, Thyroid Hormone; D055758:SOX9 Transcription Factor; C504918:Sox9a protein, zebrafish; C504919:Sox9b protein, zebrafish; D015534:Trans-Activators; D014157:Transcription Factors; D029961:Zebrafish Proteins