Biotransformation of (-)-menthone by human liver microsomes.
The aim of the current study was to investigate the metabolism of (-)-menthone by liver microsomes of humans. (-)-Menthone (1) was metabolized to (+)-neomenthol (2) (3-reduction) and 7-hydroxymenthone (3) by human liver microsomes. The metabolites formed were analyzed on GC and GC-MS. Kinetic analysis showed that K(m) and V(max) values for the metabolized (-)-menthone to respective (+)-neomenthol and 7-hydroxymenthone by liver microsomes of human sample HG70 were 0.37 mM and 4.91 nmol/min/mg protein and 0.07 mM and 0.71 nmol/min/mg protein.
Miyazawa
Mitsuo
M
Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, Osaka, Japan. miyazawa@apch.kindai.ac.jp
Nakanishi
Kyousuke
K
D001711:Biotransformation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007700:Kinetics; D008610:Menthol / Q000032:analysis / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*
D008610:Menthol; C019466:menthone