Analysis of loss of heterozygosity and immunohistochemistry in BRCA1 gene in sporadic breast cancers.
BRCA1 is a tumour suppressor gene (TSG), which predisposes cancer to both breast and ovary. The primary objective of the present study is to ascertain the involvement of BRCA1 gene in the pathogenesis of sporadic breast cancer women in Chennai (South India) by analysing its protein expression by immunohistochemistry (IHC) and loss of heterozygosity (LOH) for confirmation of the involvement of TSG in the study population. We found down regulation of BRCA1 protein (54%) in IHC and it was correlated with the clinicopathological parameters of the patients. We found near significant correlation (P < 0.063) between BRCA1 protein expression and clinicopathological parameters. We found 30% LOH in our study and it was also correlated with the clinicopathological parameters. No correlation was found between LOH and clinicopathological parameters. Though we found no correlation, the results revealed in this study support the involvement of BRCA1 TSG in the pathogenesis of sporadic breast cancer women in Chennai (South India).
Dinesh
Kada Peela Bheeman
KP
Unit of Biochemistry, Department of Zoology, University of Madras, Guindy Campus, Chennai, 600 025, India.
Devaraj
Halagowder
H
Murugan
Vadivel
V
Rajaraman
Ramamurthy
R
Niranjali
Sivasidhambaram
S
D019313:BRCA1 Protein / Q000032:analysis* / Q000235:genetics; D001943:Breast Neoplasms / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D019398:Genes, BRCA1; D006801:Humans; D007150:Immunohistochemistry; D007194:India / Q000453:epidemiology; D019656:Loss of Heterozygosity
D019313:BRCA1 Protein