title: Effects of 4-n-nonylphenol and tamoxifen on salmon gonadotropin-releasing hormone, estrogen receptor, and vitellogenin gene expression in juvenile rainbow trout.
abstract: Alkylphenols such as nonylphenol (NP) are one of a wide variety of environmental chemicals reported to have estrogenic effects in both in vitro and in vivo studies. Induction of hepatic vitellogenin (Vg) gene expression is widely used as a biomarker for xenoestrogen exposure in fish. However, little work has been done to characterize the molecular effects of xenoestrogens on other potential target organs such as the brain. To evaluate brain and liver effects of 4-n-nonylphenol (4-NP), juvenile rainbow trout (Oncorhynchus mykiss) were exposed to waterborne 4-NP or 17beta-estradiol (E(2)). Changes in mRNA levels of salmon gonadotropin-releasing hormone (sGnRH) and estrogen receptor alpha (ERalpha) isoforms in the brain and ERalpha isoforms and Vg in the liver were measured after 3 and 6 days of exposure, with the help of a relative RT-PCR-based quantification method. Fish were treated with increasing doses of 4-NP ranging from 0.01 to 10 microM (2.2 microg/l to 2.2 mg/l), and results were compared to the effect of E(2) or tamoxifen, a specific ER modulator. In liver, E(2) and the highest doses of 4-NP increased Vg and ERalpha long-isoform mRNA levels within 3 or 6 days of exposure, but 4-NP did not have any effect on ERalpha short-isoform transcription level. In the brain, 4-NP reduced sGnRH2 gene expression in a dose-dependent manner, but did not modify sGnRH1 or ERalpha mRNA levels.
authors.affiliation: MRC Toxicology Unit, University of Leicester, Leicester LE1 9HN, United Kingdom. angelique.vetillard@ipbs.fr
authors.forename: Angelique
authors.initials: A
authors.lastname: Vetillard
authors.forename: Thierry
authors.initials: T
authors.lastname: Bailhache
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D020845:Selective Estrogen Receptor Modulators / Q000633:toxicity; D013629:Tamoxifen / Q000633:toxicity; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D010636:Phenols; D012333:RNA, Messenger; D020845:Selective Estrogen Receptor Modulators; D014819:Vitellogenins; D013629:Tamoxifen; D007987:Gonadotropin-Releasing Hormone; C041594:4-nonylphenol