Binding studies of an arabinogalactan-protein from Echinacea purpurea to leucocytes.
Flow cytometric investigations show binding of an isolated arabinogalactan-protein (AGP) from pressed juice of the aerial parts of Echinacea purpurea to the cell surface of human leucocytes. AGP demonstrates binding to lymphocytes, monocytes and granulocytes of different donors (n=8). Competition assays with two antibodies, directed against CD4 and CD8, revealed no interaction of AGP with these receptors, leading to the conclusion that binding of AGP to leucocytes is mediated via other structures.
Thude
S
S
Department of Pharmaceutical Biology, Institute of Pharmacy, University of Kiel, Gutenbergstr. 76, 24118 Kiel, Germany.
Classen
B
B
Blaschek
W
W
Barz
D
D
Thude
H
H
D020900:Echinacea; D005434:Flow Cytometry; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D009088:Mucoproteins / Q000378:metabolism*; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding
D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins