Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin.
We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (Kd) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.
Kakita
Mai
M
Institute for Antibodies Ltd., Toyoake, Aichi 470-1192, Japan.
Takahashi
Tsuyoshi
T
Komiya
Takako
T
Iba
Yoshitaka
Y
Tsuji
Takao
T
Kurosawa
Yoshikazu
Y
Takahashi
Motohide
M
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D004167:Diphtheria Toxin / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011817:Rabbits
D000911:Antibodies, Monoclonal; D004167:Diphtheria Toxin