title: Babesia microti primarily invades mature erythrocytes in mice.
abstract: Babesia microti is a tick-borne red blood cell parasite that causes babesiosis in people. Its most common vertebrate reservoir is the white-footed mouse. To determine whether B. microti invades reticulocytes, as does the canine pathogen B. gibsoni, we infected the susceptible inbred mouse strains C.B-17.scid and DBA/2 with a clinical isolate of B. microti. Staining of fixed permeabilized red blood cells with 4',6'-diamidino-2-phenylindole or YOYO-1, a sensitive nucleic acid stain, revealed parasite nuclei as large bright dots. Flow cytometric analysis indicated that parasite DNA is primarily found in mature erythrocytes that expressed Babesia antigens but not the transferrin receptor CD71. In contrast, CD71-positive reticulocytes rarely contained Babesia nuclei and failed to express Babesia antigens. Accordingly, the frequency of YOYO-1-positive, CD71-negative cells strongly correlated with parasitemia, defined as the frequency of infected red blood cells assessed on Giemsa-stained blood smears. Importantly, the absolute numbers generated by the two techniques were similar. Parasitemia was modest and transient in DBA/2 mice but intense and sustained in C.B-17.scid mice. In both strains, parasitemia preceded reticulocytosis, but reticulocytes remained refractory to B. microti. In immunocompetent C.B-17 mice, reticulocytosis developed early, despite a marginal and short-lived parasitemia. Likewise, an early reticulocytosis developed in resistant BALB/cBy and B10.D2 mice. These studies establish that B. microti has a tropism for mature erythrocytes. Although reticulocytes are rarely infected, the delayed reticulocytosis in susceptible strains may result from parasite or host activities to limit renewal of the mature erythrocyte pool, thereby preventing an overwhelming parasitemia.
authors.affiliation: Tufts-New England Medical Center, Division of Geographic Medicine and Infectious Diseases, Box #41, 750 Washington St., Boston, MA 02111, USA.
authors.forename: Ingo
authors.initials: I
authors.lastname: Borggraefe
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.forename: Sam R
authors.initials: SR
authors.lastname: Telford
authors.forename: Sanjay
authors.initials: S
authors.lastname: Menon
authors.forename: Rouette
authors.initials: R
authors.lastname: Hunter
authors.forename: Sohela
authors.initials: S
authors.lastname: Shah
authors.forename: Andrew
authors.initials: A
authors.lastname: Spielman
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Gelfand
authors.forename: Henry H
authors.initials: HH
authors.lastname: Wortis
authors.forename: Edouard
authors.initials: E
authors.lastname: Vannier
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D041001:Babesia microti / Q000276:immunology / Q000472:pathogenicity*; D001583:Benzoxazoles; D004912:Erythrocytes / Q000469:parasitology*; D005434:Flow Cytometry; D008206:Lymphatic Diseases / Q000209:etiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016513:Mice, SCID; D011806:Quinolinium Compounds; D011990:Receptors, Transferrin / Q000032:analysis
chemical_list: D015703:Antigens, CD; D001583:Benzoxazoles; C068126:CD71 antigen; D011806:Quinolinium Compounds; D011990:Receptors, Transferrin; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium