Sequence analysis for the complete proviral genome of reticuloendotheliosis virus Chinese strain HA9901.
The genomic DNA extracted from chicken embryo fibroblast (CEF) infected with a Chinese field isolate HA9901 of reticuloendotheliosis virus (REV) was used as the template to amplify the REV proviral genomic cDNA by PCR with 6 pairs of primers according to published sequences. Six overlapping fragments were amplified, cloned into the TA vector and sequenced, including a fragment which was amplified from the circular proviral cDNA and covering both 5'- and 3'-ends. The complete sequence of the whole genome was established and analyzed with a DNAstar software. Comparisons of the sequence with two other strains demonstrated that the genomes of REV were relatively conservative, the homogenecity for all genes or LTR fragments of the 3 strains was over 92%, no matter whether they were isolated from different species and regions in different years. But, the homology of Chinese strain HA9901 to a fowl pox virus-associated strain from Chickens was higher than that to strain SNV isolated from ducks.
Wang
Yu
Y
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
Cui
Zhizhong
Z
Jiang
Shijin
S
D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D005347:Fibroblasts / Q000166:cytology / Q000821:virology; D019084:Fluorescent Antibody Technique, Indirect; D023061:Gene Order; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D011533:Proviruses / Q000235:genetics*; D001356:Reticuloendotheliosis virus / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins