title: POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila.
abstract: Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N-terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and Drosophila. Here, we show that POSH forms a complex with Apoptosis-linked gene-2 (ALG-2) and ALG-2-interacting protein (ALIX/AIP1) in a calcium-dependent manner. Overexpression of ALG-2 or ALIX in developing imaginal eye discs resulted in roughened or melanized eyes, respectively. These phenotypes were enhanced by co-overexpression of POSH. We found that overexpression of either gene could induce ectopic JNK activation, suggesting that POSH/ALG-2/ALIX may function together in the regulation of the JNK pathway.
authors.affiliation: Department of Biological Sciences, Tokyo Metropolitan University, Hachioji-shi, Tokyo 192-0397, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Tsuda
authors.forename: Ki-Hyeon
authors.initials: KH
authors.lastname: Seong
authors.forename: Toshiro
authors.initials: T
authors.lastname: Aigaki
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005123:Eye / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D047468:Immunoprecipitation; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism
chemical_list: C511870:ALIX protein, Drosophila; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C453299:POSH protein, Drosophila; D012333:RNA, Messenger; D048670:MAP Kinase Kinase 4