title: [Advanced oxidation protein products in diabetic nephropathy patients and its relation with superoxide dismutase, glutathione peroxidase, neopterin].
abstract: OBJECTIVE
  To determine the changes of advanced oxidation protein products (AOPP) in diabetic nephropathy (DN) patients, as well as its relationship with superoxide dismutase (SOD), glutathione peroxidase (GPx) and neopterin (NPT).
  
  
  METHODS
  By the concentration of urinary albumin excretion rate (UAER) and creatinine (Cr), 85 diabetes mellitus (DM) patients were divided into 4 groups as non-DN group (DM), early-staged DN group (DN3), significant DN group (DN4) and end-staged DN group (DN5). The concentration of the serum AOPP was measured by ameliorated method introduced by Wikto-Sarsat, while SOD by Xanthine oxidase test, GPx by [5,5'Dithio-bis (2-Nitrobenzoic aicd) ] (DTNB) reaction test and NPT by ELISA.
  
  
  RESULTS
  AOPP in Group DN5 [(117.8 +/- 64.8) [micromol/L] and Group DN4 [ (80.0 +/- 23.0) micromol/L] were significantly higher than those in Group DM [(58.2 +/- 17.7) micromol/L]. There was no significant difference of AOPP between Group DN3 [(72.7 +/-17.2) micromol/L] and Group DM. Serum AOPP negatively correlated with SOD and GPx (r = -0.217 and -0.374 respectively, P < 0.05), while positively correlated with NPT (r = 0.499, P < 0.01).
  
  
  CONCLUSIONS
  DN patient has enhanced protein oxidation than DM patient, which is related to oxidative stress and chronic inflammation in DN.
authors.affiliation: Department of Endocrinology, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350025, China.
authors.forename: Zhi-xian
authors.initials: ZX
authors.lastname: Wu
authors.forename: Yao-ming
authors.initials: YM
authors.lastname: Xue
authors.forename: Chen-zhong
authors.initials: CZ
authors.lastname: Li
authors.forename: Mei-ping
authors.initials: MP
authors.lastname: Guan
mesh_terms: D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D003928:Diabetic Nephropathies / Q000097:blood*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019798:Neopterin / Q000097:blood*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D019798:Neopterin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase