Changes of expression of stretch-activated potassium channel TREK-1 mRNA and protein in hypertrophic myocardium.
The expression of stretch-activated potassium channel TREK-1 mRNA and protein of hypertrophic myocardium was measured. Using a model of hypertrophy induced by coarctation of abdominal aorta in male Wistar rats, the expression of TREK-1 mRNA and protein was detected by using semi-quantitative RT PCR and Western blot respectively. At 4th and 8th week after constriction of the abdominal aorta, rats developed significant left ventricular hypertrophy. As compared to sham-operated group, stretch-activated potassium channel TREK-1 mRNA was strongly expressed and protein was up-regulated in operation groups (P < 0.05). It was concluded that the expression of TREK-1 was up-regulated in hypertrophic myocardium induced by chronic pressure overload in Wistar rats.
Cheng
Longxian
L
Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Su
Fengcheng
F
Ripen
Nsenga
N
Fan
Hong
H
Huang
Kai
K
Wang
Min
M
Peng
Hongyu
H
Mei
Chunli
C
Zhao
Fang
F
Liao
Yuhua
Y
D000818:Animals; D001012:Aorta, Abdominal; D003250:Constriction; D006984:Hypertrophy; D017379:Hypertrophy, Left Ventricular / Q000209:etiology / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D024683:Potassium Channels, Tandem Pore Domain / Q000096:biosynthesis* / Q000235:genetics; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation; D020257:Ventricular Remodeling
D024683:Potassium Channels, Tandem Pore Domain; D011506:Proteins; D012333:RNA, Messenger; C103742:potassium channel protein TREK-1