Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection.
A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.
Carnieli Junior
Pedro
P
Institute Pasteur São Paulo, São Paulo, SP, Brazil. pecarniel@yahoo.com.br
Ventura
Armando Moraes
AM
Durigon
Edison Luiz
EL
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D002685:Chiroptera; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D004076:Digoxigenin; D004285:Dogs; D005814:Genes, Viral / Q000235:genetics*; D006736:Horses; D006801:Humans; D017403:In Situ Hybridization; D008163:Luminescent Measurements; D009698:Nucleoproteins / Q000235:genetics*; D011818:Rabies / Q000175:diagnosis* / Q000821:virology; D011820:Rabies virus / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012756:Sheep
D015342:DNA Probes; D004279:DNA, Viral; D009698:Nucleoproteins; D004076:Digoxigenin