[Effect of hematopoietic stimulating factors on the expansion of megakaryocyte].
OBJECTIVE
To investigate the effect of hematopoietic stimulating factors on the expansion of mature megakaryocytes.


METHODS
(2, 4, 6, 8, 10) x 10(5)/mL bone marrow single nucleus cells (BMNC) were added in the culture system of colony forming unit-megkaryocyte (CFU-Meg) to find out the relationship of the cultured BMNC with the output of CFU-Meg. rmSCF + rmTPO + rmIL-3 (3HSFs) and rmSCF + rmTPO + rmIL-3 + rmIL-6 (4HSFs) or F-CM were added in the liquid culture system of megkaryocytes respectively. The number of mature megakaryocytes were counted every other day.


RESULTS
The number of CFU-Meg increased with the increase of the cultured BMNC. The CFU-Meg productivity of 1 x 10(6) BMNC/mL culture system was more than that of 2 x 10(5) BMNC/mL culture system. 3HSFs and 4HSFs or F-CM significantly promoted the expansion of mature megakaryocytes in the liquid culture system, but the effect was different. The peak time of the number of mature megakaryocytes in 3HSFs and 4HSFs or F-CM were 7 d, 7 d and 5 d respectively.


CONCLUSIONS
3HSFs and 4 HSFs or F-CM had positive effect on the expansion of mature megakaryocytes. 4HSFs was better than 3HSFs and F-CM. 3HSFs was better than F-CM. The peak time of the number of mature megakaryocytes in different culture systems was different.
Huang
Yan-hong
YH
Research Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha, China. tjyanhong@yahoo.com.cn
Wang
Qi-ru
QR
D000818:Animals; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008297:Male; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice
D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D015850:Interleukin-6; D016173:Macrophage Colony-Stimulating Factor