title: Tween 40-based precipitate production observed on modified chromogenic agar and development of biological identification kit for Malassezia species.
abstract: We developed a simple identification kit for nine species of Malassezia (M. furfur, M. slooffiae, M. sympodialis, M. restricta, M. obtusa, M. globosa, M. pachydermatis, M. dermatis, and M. japonica) based on their biological features. This method utilizes Tween 40-based precipitate production on modified chromogenic agar (CHROMagar) Malassezia medium, growth on specific agars (Sabouraud's dextrose agar, Cremophor EL agar, Tween 60-esculin agar), and catalase reactions. This identification kit was verified with 11 type and reference strains of nine Malassezia species. An additional 26 clinical isolates were also successfully identified using the kit and the results were confirmed by molecular biological analysis.
authors.affiliation: Kanto Chemical Co., Inc., Nihonbashi Honcho, Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Kaneko
authors.forename: Koichi
authors.initials: K
authors.lastname: Makimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugita
authors.forename: Hideyo
authors.initials: H
authors.lastname: Yamaguchi
mesh_terms: D000362:Agar; D011232:Chemical Precipitation; D003470:Culture Media; D006801:Humans; D008290:Malassezia / Q000254:growth & development / Q000302:isolation & purification*; D011136:Polysorbates / Q000737:chemistry*; D011933:Reagent Kits, Diagnostic
chemical_list: D003470:Culture Media; D011136:Polysorbates; D011933:Reagent Kits, Diagnostic; D000362:Agar; C068430:polysorbate 40