title: Characterization of truncated hantavirus nucleocapsid proteins and their application for serotyping.
abstract: Hemorrhagic fever with renal syndrome (HFRS) is a fulminant infectious disease characterized by fever, hemorrhage, renal impairment, and thrombocytopenia. Hantaviruses associated with this belong to different serotypes: Hantaan (HTN), Seoul (SEO), Dobrava/Belgrade (DOB), and Puumala (PUU). The first two, HTN and SEO, are endemic in China. To investigate the epidemiology of HFRS and virus transmission in China, we constructed prokaryotic plasmids encoding truncated recombinant HTN and SEO nucleocapsid proteins (NPs), which lacked 154 amino acid (aa), 99 aa, or 49 aa in the N-terminal region, respectively. After expression, the truncated rNPs were tested as serotyping antigens, particularly for use in the enzyme-linked immunosorbent assay (ELISA). In addition, 68 acute and 52 convalescent sera were collected from HFRS patients from Harbin, Lantian, and Kaifeng regions in China in 2004, which had hantavirus specific antibodies by IFA. A neutralization test was used to differentiate these, which showed that 73 were due to HTN infection, 33 to SEO infection, and 14 undetermined. By ELISA, the truncated rNPs, that lacked 99 (rNP100) or 49 (rNP50) N-terminal amino acids of the NPs of HTN and SEO, were able to differentiate HTNV and SEOV-specific immune sera, but the rNP155 could not. Particularly, the ELISAs based on the rNP50s had a result comparable to PRNT. Thus, the rNP50 is recommended as efficient serotyping antigen for hantavirus infection diagnosis by ELISA.
authors.affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.forename: Danlei
authors.initials: D
authors.lastname: Mou
authors.forename: Yanping
authors.initials: Y
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xinhong
authors.initials: X
authors.lastname: Li
authors.forename: Junping
authors.initials: J
authors.lastname: Ren
authors.forename: Pingzhong
authors.initials: P
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhansheng
authors.initials: Z
authors.lastname: Jia
authors.forename: Changxing
authors.initials: C
authors.lastname: Huang
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Sun
authors.forename: Weisong
authors.initials: W
authors.lastname: Yang
authors.forename: Shu-Yuan
authors.initials: SY
authors.lastname: Xiao
authors.forename: Xuefan
authors.initials: X
authors.lastname: Bai
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018063:Hantaan virus / Q000145:classification / Q000235:genetics / Q000276:immunology; D006476:Orthohantavirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D006480:Hemorrhagic Fever with Renal Syndrome / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D051379:Mice; D009500:Neutralization Tests; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D029263:Seoul virus / Q000145:classification / Q000235:genetics / Q000276:immunology; D012703:Serotyping / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins