Structural studies of the O-antigenic oligosaccharide from Vibrio salmonicida strain C2 isolated from Atlantic cod, Gadus morhua L.
We report the chemical structure of the oligosaccharide part of Vibrio salmonicida lipopolysaccharide serotype C2, isolated from Atlantic cod (Gadus morhua L.). The structure was established by NMR spectroscopy and mass spectrometry. It is concluded that the oligosaccharide has the following structure in which L-alpha-D-Hepp is L-glycero-alpha-D-manno-heptopyranose, D-alpha-D-Hepp is D-glycero-alpha-D-manno-heptopyranose, alpha-NonA is 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-D-galacto-nonulosonic acid, and PEA is phosphoethanolamine. [chemical structure: see text]
Bøgwald
Jarl
J
Norwegian College of Fishery Science, University of Tromsø, Norway. jarlb@nfh.uit.no
Hoffman
James
J
D044165:Aliivibrio salmonicida / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D002240:Carbohydrate Sequence; D048389:Gadus morhua / Q000382:microbiology*; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides