Annotation of unknown yeast ORFs by correlation analysis of microarray data and extensive literature searches.
Changes in the expression of genes were used to elucidate the metabolic pathways and regulatory mechanisms that respond to environmental or genetic modifications. Results from previously published chemostat datasets were merged with novel data generated in the present study. ORFs displaying significant changes in expression that correlated with those of other ORFs were analysed using GO mapping tools and supplemented by literature information. The strategy developed was used to propose annotations for ORFs of unknown function. The following ORFs were assigned functions as a result of this study: YMR090w, YGL157w, YGR243w, YLR327c, YER121w, YFR017c, YGR067c, YKL187c, YGR236c (SPG1), YMR107w (SPG4), YMR206w, YER067w, YJL103c, YNL175C (NOP13) YJL200C, YDL070C (FMP16) and YGR173W.
Pir
Pinar
P
Department of Chemical Engineering, Bogaziçi University, Bebek 34342, Istanbul, Turkey.
Ulgen
Kutlu O
KO
Hayes
Andrew
A
Ilsen Onsan
Z
Z
Kirdar
Betül
B
Oliver
Stephen G
SG
D000465:Algorithms; D019295:Computational Biology; D030541:Databases, Genetic; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology*; D016681:Genome, Fungal / Q000235:genetics / Q000502:physiology*; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames / Q000235:genetics / Q000502:physiology*; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012331:RNA, Fungal