Folate immobilized and PEGylated adenovirus for retargeting to tumor cells.
The surface of adenovirus (ADV) was modified with folate-poly(ethylene glycol) (FOL-PEG) conjugate to overcome the inherent problems associated with viral gene therapy. The ADV coding the green fluorescent protein (GFP) gene was used to immobilize PEG or FOL-PEG conjugate on the viral surface to comparatively evaluate their extents of retargeting and immune response, as compared to those of naked ADV. The FOL-PEG immobilized ADV exhibited a greatly enhanced level of GFP expression than naked or PEG immobilized ADV for a folate receptor overexpressing cell line (KB cells), but not for a folate receptor deficient cell line (A549 cells), suggesting that the retargeting of ADV could be achieved by immobilizing FOL-PEG conjugate. The ADV immobilized with PEG or FOL-PEG also significantly lowered innate immune response, as judged from the amount of interleukin 6 released from macrophage cells.
Oh
In Kyung
IK
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea.
Mok
Hyejung
H
Park
Tae Gwan
TG
D000256:Adenoviridae / Q000737:chemistry* / Q000502:physiology*; D000818:Animals; D045744:Cell Line, Tumor; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D009369:Neoplasms / Q000473:pathology* / Q000821:virology*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011092:Polyethylene Glycols; D005492:Folic Acid