title: Liver exposure to xenobiotics: the aging factor and potentials for functional foods.
abstract: Hepatocytes isolated from 20- and 4-month Wistar rats and cultured with or without alpha-linolenic acid (LNA) were then added with nutraceutical YHK or sylibin before the test with iron or copper. Overall, YHK proved to be more effective than sylibin in Fe/Cu-induced peroxidative damage on normal and LNA-loaded hepatocytes (p < 0.05). YHK exerted a significant protection against DPPH radical-scavenging activity in the "old" group (p versus sylibin) and against lipophilic generators in both age groups (p < 0.05 versus sylibin). Both compounds were ineffective on age-related increase of surface-charge density. These preliminary data suggest that age per se enhances the vulnerability of hepatocytes to xenobiotics, whereas some safe nutraceuticals seem to exert significant protective effects.
authors.affiliation: Hepato-Gastroenterology Department, San Giuseppe Hospital, Milan, Italy. fmarchimede@libero.it
authors.forename: F
authors.initials: F
authors.lastname: Marotta
authors.forename: P
authors.initials: P
authors.lastname: Lecroix
authors.forename: M
authors.initials: M
authors.lastname: Harada
authors.forename: K
authors.initials: K
authors.lastname: Masulair
authors.forename: P
authors.initials: P
authors.lastname: Safran
authors.forename: A
authors.initials: A
authors.lastname: Lorenzetti
authors.forename: S K
authors.initials: SK
authors.lastname: Ono-Nita
authors.forename: P
authors.initials: P
authors.lastname: Marandola
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019587:Dietary Supplements; D022781:Hepatocytes; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine; D015262:Xenobiotics / Q000494:pharmacology*; D017962:alpha-Linolenic Acid
chemical_list: D010936:Plant Extracts; D015262:Xenobiotics; D017962:alpha-Linolenic Acid; D012710:Serum Albumin, Bovine