title: [Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC].
abstract: OBJECTIVE
  To determine 20(S)-ginsengnoside Rh2 in the hydrolysis product of saponins from leaves of Panax qinquefolium.
  
  
  METHODS
  The separation was performed on ZORBAX EXEND C18 column (4.6 mm x 250 mm, 5 microm), eluted with methanol and water (85:15) as mobile phase with the rate of 1.2 mL x min(-1) at 25 degrees C, the wavelength for measurement was 203 nm.
  
  
  RESULTS
  The calibration curve was linear in the range of 0.5-25 microg for 20(S)-ginsengnoside Rh2(r = 0.9999, n = 7). The average recovery was 99.7% (RSD= 1.0%).
  
  
  CONCLUSIONS
  This method is simple, accurate, reliable and reproducible. The result shows that the transform ratio of 20(S)-ginsengnoside Rh2 is high by this hydrolysis method.
authors.affiliation: College of Chemistry, Jilin Unirersity, Changchun, China. xwli@jlu.edu.cn
authors.forename: Xu-wen
authors.initials: XW
authors.lastname: Li
authors.forename: Ming-yu
authors.initials: MY
authors.lastname: Gui
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yong-ri
authors.initials: YR
authors.lastname: Jin
authors.forename: Han-qi
authors.initials: HQ
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D036145:Ginsenosides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D005894:Panax / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D036145:Ginsenosides; C055305:ginsenoside Rh2