Cryopreservation of somatic embryos of the herbaceous peony (Paeonia lactiflora Pall.) by air drying.
This study was carried out to establish a suitable method for the cryopreservation of somatic embryos of the herbaceous peony. The somatic embryos were obtained from cotyledon and anther cultures on a MS medium supplemented with abscisic acid (ABA) and phenylacetic acid (PAA), respectively. The frequency of somatic embryo formation was the greatest (61%) from the cotyledons cultured on a MS medium supplemented with 1.0 mg l(-1) of ABA. Embryos were also obtained directly from anthers cultured on a MS medium with or without 2.0 mg l(-1) of PAA. For the cryopreservation of peony somatic embryos, the embryos were dried under a stream of sterile air and frozen by immersion in liquid nitrogen. Thawed embryos were germinated into plantlets after placing on a medium containing 0.3 mg l(-1) of gibberellic acid (GA(3)). The frequency of the post-thaw regrowth of cryopreserved somatic embryos was related to their size and desiccation time, the latter ranging from 0 to 2 h. When the somatic embryos were desiccated for 1 h, the frequency of post-thaw regrowth was greater than 66%. The frequency of post-thaw regrowth of the cryopreserved somatic embryos from anthers and cotyledon tissues was generally high when they were 2-3 mm in size. Desiccation may be a suitable method for the cryopreservation of somatic embryos of the herbaceous peony.
Kim
Hyun Mi
HM
Department of Agronomy, Kyungpook National University, Daegu, Republic of Korea.
Shin
Jong Hee
JH
Sohn
Jae Keun
JK
D000040:Abscisic Acid / Q000494:pharmacology; D000388:Air; D018548:Cotyledon / Q000196:embryology; D015925:Cryopreservation / Q000379:methods*; D003890:Desiccation / Q000379:methods; D035264:Flowers / Q000196:embryology; D005875:Gibberellins / Q000494:pharmacology; D029596:Paeonia / Q000187:drug effects / Q000196:embryology*; D010648:Phenylacetates / Q000494:pharmacology
D005875:Gibberellins; D010648:Phenylacetates; D000040:Abscisic Acid; C007842:gibberellic acid; C025136:phenylacetic acid