title: IL-5 up-regulates the expression of TGF-beta1 in human blood eosinophils in vitro.
abstract: To investigate the effects of IL-5 on the expression of TGF-beta1 in eosinophils in vitro, eosinophils were incubated in the presence of the same concentrations of 1L-4, IL-5 and IFNgamma, different concentrations of IL-5 in vitro and changes of eosinophil viability were assessed by trypan blue exclusion. Non-cytokine was employed as a negative control. 16 h after the cultivation, supernatants and cells were assayed by using TGF-beta1 specific ELISA and RT-PCR. The mRNA expression and protein expresssion of TGF-beta1 in eosinophils stimulated with different cytokines was observed. The expression of TGF-beta1 protein in eosinophils was increased significantly by IL-4 (433.67 +/- 9.86 vs 228.9 +/- 2.87) and IL-5 (403.72 +/- 7.60 vs 228.9 +/- 2.87, P < 0.05), while decreased by IFNgamma (178.47 +/- 2.60 vs 228.9 +/- 2.87). At the same time, the results demonstrated that the basal level of TGF expression was enhanced by IL-5 in all samples (P < 0.05). The expression of TGF-beta1 mRNA was 1.42, 1.70, 1.76-folds higher than that of the non-stimulated controls. It is concluded that IL-5 can up-regulate the expression of TGF-beta1 in eosinophils in vitro, which might have effect in eosinophil-associated chronic rejection.
authors.affiliation: Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huzhong University of Science and Technology, Key Laboratory of Organ Transplantation, Ministry of Education and Health, Wuhan 430030, China.
authors.forename: Yabing
authors.initials: Y
authors.lastname: Huang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D004804:Eosinophils / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D015848:Interleukin-5; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta