[The effect of immobilized fibronectin on karyotypic variability in the skin fibroblast cell subline of indian muntjac].
The numerical and structural karyotypic variability has been investigated in the Indian muntjac skin fibroblast cell subline MT on cultivating cells on the fibronectin-coated surface. In cell subline MT, cultivated on the fibronectin-coated surface for 1 and 2 days, the character of cell distribution for the chromosome number did not change. In 3, 4 and 8 days, the character of cell distribution for the chromosome number changed. These changes involve a significant decrease in frequency of cells with modal numbers of chromosomes, and an increase in frequency of cells with lower chromosome numbers. Many new additional structural variants of the karyotype (SVK) appear. The observed alterations seem to be due to both disturbances of mitotic apparatus and selection of SVK, which are more advantageous to changed culture conditions of the cell population. Detachment of cells from the fibronectin-coated surface, followed by a 1 day cultivation on a hydrophilic surface, commonly used for routine cell cultivation, does not restore the control cell distribution for the chromosome number, but cultivation in these conditions for 5 days restore control distribution. The frequency of chromosomal aberrations on cultivation on the fibronectin-coated surface for 3 and 4 days significantly increases, mainly at the expence of dicentrics (telomeric association). On prolongating the time of cultivation up to 8 days on the fibronectin-coated surface the frequency of chromosomal aberrations approaches the control value. Structural instability of chromosomes at cultivation on the fibronectin-coated surface demonstrates nonspecific reaction of "markerless" cell lines to unfavourable factors of the environment. We discuss possible reasons of differences in the character of karyotypic variability in cell lines of the Indian muntjac skin fibroblasts on cultivating on laminin and fibronectin.
Polianskaia
G G
GG
Goriachaia
T S
TS
Pinaev
G P
GP
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002869:Chromosome Aberrations / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure*; D005353:Fibronectins / Q000494:pharmacology*; D014644:Genetic Variation; D007621:Karyotyping; D018668:Muntjacs / Q000235:genetics*; D012867:Skin / Q000187:drug effects / Q000648:ultrastructure*
D005353:Fibronectins