Development of a ratiometric fluorescent zinc ion probe in near-infrared region, based on tricarbocyanine chromophore.
Novel ratiometric fluorescent probes for Zn2+ in the near-infrared region, based on a tricarbocyanine chromophore, have been designed, synthesized, and evaluated. Upon addition of Zn2+, a 44 nm red shift of the absorption maximum was observed, which indicates that this probe could work as a ratiometric probe for Zn2+. This change is due to the difference in the electron-donating ability of the amine substituent before and after reaction with Zn2+. This fluorescence modulation of amine-substituted tricarbocyanines should be applicable to dual-wavelength measurement of various biomolecules or enzyme activities.
Kiyose
Kazuki
K
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Kojima
Hirotatsu
H
Urano
Yasuteru
Y
Nagano
Tetsuo
T
D000476:Alkanesulfonates / Q000737:chemistry; D002232:Carbocyanines / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007259:Infrared Rays; D015032:Zinc / Q000032:analysis*
D000476:Alkanesulfonates; D002232:Carbocyanines; D004396:Coloring Agents; D005456:Fluorescent Dyes; C520963:dipicolylcyanine; D015032:Zinc