Isolation and identification of mixed linked beta -glucan degrading bacteria in the intestine of broiler chickens and partial characterization of respective 1,3-1,4-beta -glucanase activities.
Media with 1,3-1,4-beta -glucans as selective markers were used for isolation of non-starch-polysaccharide (NSP) degrading bacteria from the intestinal tract of broiler chicken. Formerly unknown 1,3-1,4-beta endoglucanase activities in various bacterial species were identified in this study. E. faecium , Streptococcus , Bacteroides and Clostridium strains seem to be responsible for degradation of mixed linked beta -glucans in the small intestine and in the hind gut of chickens. Strict anaerobic bacteria (Bacteroides ovatus , B. uniformis , presumably B. capillosus and Clostridium perfringens ) as well as an unidentified bacterium with 98% 16S rDNA homology to an uncultered chicken cecum bacterium were isolated. Additionally, Streptococcus bovis with 1,3-1,4-beta -endoglucanase activity was also detected. Different 1,3-1,4-beta -endoglucanase activity profiles were observed in SDS/PAGE zymograms.
Beckmann
Lutz
L
Institute for Animal Nutrition, Faculty of Veterinary Medicine, Free University of Berlin, Brümmerstr, Berlin, Germany.
Simon
Ortwin
O
Vahjen
Wilfried
W
D000818:Animals; D001439:Bacteroides / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens / Q000378:metabolism / Q000382:microbiology*; D003016:Clostridium perfringens / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D016984:Enterococcus faecium / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology; D008297:Male; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D016985:Streptococcus bovis / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D047071:beta-Glucans / Q000378:metabolism*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D047071:beta-Glucans; D006026:Glycoside Hydrolases; C028020:licheninase