title: Crystal structure of the DNA-binding domain of BldD, a central regulator of aerial mycelium formation in Streptomyces coelicolor A3(2).
abstract: BldD is a central regulator of the developmental process in Streptomyces coelicolor. The 1.8 angstroms resolution structure of the DNA-binding domain of BldD (BldDN) reveals that BldDN forms a compact globular domain composed of four helices (alpha1-alpha4) containing a helix-turn-helix motif (alpha2-alpha3) resembling that of the DNA-binding domain of lambda repressor. The BldDN/DNA complex model led us to design a series of mutants, which revealed the important role of alpha3 and the 'turn' region between alpha2 and alpha3 for DNA recognition. Based on the fact that BldD occupies two operator sites of bldN and whiG and shows significant disparity in the affinity toward the two operator sites when they are disconnected, we propose a model of cooperative binding, which means that the binding of one BldD dimer to the high affinity site facilitates that of the second BldD dimer to the low affinity site. In addition, structural and mutational investigation reveals that the Tyr62Cys mutation, found in the first-identified bldD mutant, can destabilize BldD structure by disrupting the hydrophobic core.
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: In-Kwon
authors.initials: IK
authors.lastname: Kim
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Lee
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Kim
authors.forename: Jeong-Mok
authors.initials: JM
authors.lastname: Kim
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Kim
authors.forename: Hyung-Soon
authors.initials: HS
authors.lastname: Yim
authors.forename: Sun-Shin
authors.initials: SS
authors.lastname: Cha
authors.forename: Sa-Ouk
authors.initials: SO
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D018360:Crystallography, X-Ray; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D019281:Dimerization; D005816:Genetic Complementation Test; D019077:Helix-Turn-Helix Motifs; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D048372:Streptomyces coelicolor / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics; D014443:Tyrosine / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D001426:Bacterial Proteins; C111306:BldD protein, Streptomyces coelicolor; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D014443:Tyrosine