Different results of the Salmonella umu test between three isomers of phenylenediamine (PDA) derivatives.
A post-treatment assay of the umu test was performed to detect genotoxicity of 10 phenylenediamine (PDA) derivatives using Salmonella typhimurium TA1535/pSK1002 with/without S9 mix. Seven chemicals (o-PDA, 4-chloro-o-PDA, 4-nitro-o-PDA, p-PDA, 2-chloro-p-PDA, 2-nitro-p-PDA, and 2,5-diaminotoluene) showed positive results with S9 mix, but three chemicals (m-PDA, 4-chloro-m-PDA, and 2,4-diaminotoluene) were negative with and without S9 mix. Four of 7 chemicals (o-PDA, 4-chloro-o-PDA, 4-nitro-o-PDA, and 2-nitro-p-PDA) that gave positive results with S9 mix were also positive without S9 mix. These results indicate that the genotoxicity of PDA derivative possessing m-position amino substituents was not detected in the umu post-treatment assay using TA1535/pSK1002.
Yasunaga
K
K
Mitsubishi Chemical Safety Institute Ltd., Ibaraki, Japan. k-yasunaga@ankaken.co.jp
Kiyonari
A
A
Nakagawa
M
M
Yoshikawa
K
K
D000818:Animals; D004305:Dose-Response Relationship, Drug; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000145:classification / Q000633:toxicity*; D010655:Phenylenediamines / Q000737:chemistry / Q000145:classification / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; D013014:SOS Response, Genetics / Q000187:drug effects* / Q000235:genetics; D012475:Salmonella / Q000187:drug effects* / Q000235:genetics; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D009153:Mutagens; D010655:Phenylenediamines; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins