Purification of the skeletal muscle protein Endopin 1B and characterization of the genes encoding Endopin 1A and 1B isoforms.
In the present work, a new endopin-like serpin designed mEndopin 1B was purified from bovine muscle. Biochemical characterizations (amino acid sequencing and Maldi-Tof mass spectrometry peptide mapping) demonstrated that the purified protein is different from the previously described Endopin 1, renamed mEndopin 1A. The genes and cDNA of both endopins were characterized. The cDNA sequence of mEndopin 1B encodes a predicted protein of 411 amino-acids with a molecular mass of 43808Da. The mEndopin 1B gene comprised four coding exons and an additional 5' untranslated exon. The reactive site sequence of mEndopin 1B is somewhat different from that of mEndopin 1A. Nevertheless, both serpins have a similar peptidase inhibitory pattern against examined proteases (elastase, trypsin, plasmin and chymotrypsin). The high expression of both mEndopin 1A and 1B in bovine serum and tissues and their high efficiency to inhibit elastase (k(ass) approximately 10(6)-10(7) M(-1) s(-1)) suggested that these serpins might play a major role in inflammatory processes.
Herrera-Mendez
Carlos H
CH
QuaPA, BPM, INRA de Clermont Ferrand - Theix, Saint Genès Champanelle, France.
Brémaud
Laure
L
Coulis
Gerald
G
Pélissier
Patrick
P
Sentandreu
Miguel A
MA
Aubry
Laurent
L
Delourme
Didier
D
Chambon
Christophe
C
Maftah
Abderrahman
A
Leveziel
Hubert
H
Ouali
Ahmed
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D015843:Serpins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D014357:Trypsin / Q000187:drug effects
D017931:DNA Primers; D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D020033:Protein Isoforms; C402207:SERPINA3 protein, Bos taurus; D015843:Serpins; D010196:Pancreatic Elastase; D014357:Trypsin