Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA.
Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNA(Tyr). The crystals belong to a tetragonal space group P4(1)2(1)2 with cell dimensions of a = b = 63.85 Angstrom, and c = 330.3 Angstrom. The asymmetric unit contains one molecule each of yTyrRS and tRNA(Tyr) (one-half of a 2:2 complex). X-ray diffraction data have been collected up to 2.5 Angstrom resolution.
Kusakabe
Yoshio
Y
School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
Ohno
Satoshi
S
Tanaka
Nobutada
N
Nakamura
Masashi
M
Tsunoda
Masaru
M
Moriguchi
Tomohisa
T
Asai
Norio
N
Sekine
Mitsuo
M
Yokogawa
Takashi
T
Nishikawa
Kazuya
K
Nakamura
Kazuo T
KT
D003460:Crystallization; D018360:Crystallography, X-Ray; D012365:RNA, Transfer, Tyr / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014448:Tyrosine-tRNA Ligase / Q000737:chemistry*
D012365:RNA, Transfer, Tyr; D014448:Tyrosine-tRNA Ligase