title: Co-transport of sex hormone-binding globulin/SHBG with oxytocin in transport vesicles of the hypothalamo-hypophyseal system.
abstract: The intrinsic expression of sex hormone binding globulin (SHBG) in magnocellular hypothalamic neurons, in part co-localized with either vasopressin or oxytocin, was recently described. This study is focused on the ultrastructural localization of SHBG in the hypothalamo-neurohypophyseal pathway in rats. Immunostaining for SHBG in the hypothalamic perikarya was increased by colchicine treatment, indicating that the steroid-binding globulin is subject to rapid axoplasmic transport along with the classical posterior lobe peptides. With immunoelectron-microscopic double labeling, we found co-localization of oxytocin and sex hormone binding globulin in a portion of the large dense-core vesicles in paraventricular and supraoptic perikarya and in axonal varicosities in the median eminence and in the posterior lobe. Our observations show that SHBG is processed, transported and stored along with oxytocin suggesting that SHBG is released from nerve terminals in the posterior lobe, the median eminence and possibly the brain similarly to and in conjunction with oxytocin.
authors.affiliation: Institute for Neurobiology, Ludwig-Maximilian-Universität Biozentrum, Munich, Germany. telapo@gmx.de
authors.forename: Z
authors.initials: Z
authors.lastname: Herbert
authors.forename: E
authors.initials: E
authors.lastname: Pollák
authors.forename: L
authors.initials: L
authors.lastname: Molnár
authors.forename: J D
authors.initials: JD
authors.lastname: Caldwell
authors.forename: G F
authors.initials: GF
authors.lastname: Jirikowski
mesh_terms: D000818:Animals; D005260:Female; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D010121:Oxytocin / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012738:Sex Hormone-Binding Globulin / Q000276:immunology / Q000378:metabolism*; D027981:Symporters / Q000378:metabolism*; D022161:Transport Vesicles / Q000378:metabolism*
chemical_list: D012738:Sex Hormone-Binding Globulin; D027981:Symporters; D010121:Oxytocin