[Effect of simvastatin on the proliferation of human omental preadipocytes and caspase-3 expression].
OBJECTIVE
To explore the effect of simvastatin on the cell cycle and caspase-3 expression in human omental preadipocytes.


METHODS
The preadipocytes were randomly divided into a blank group, a 10(-5) mol/L simvastatin group, and a 10(-4) mol/L simvastatin group. Each group was incubated with different concentrations of simvastatin for 48 hours. MTT method was used to analyze the effect of simvastatin on the proliferation. Distribution of the cell cycle was measured by flow cytometric. Caspase-3 expression was examined by cyto- immunochemistry.


RESULTS
After being induced to differentiate for 16 days the human omental preadipocytes developed to mature adipocyte penotypes with lipid droplet. Simvastatin 10(-4) mol/L had significant anti-proliferation effect. Flow cytometric analysis showed the cell cycle was blocked in G0/G1 phase and caspase-3 positive cells increased dramatically.


CONCLUSIONS
Simvastatin may block the cells in G0/G1 phase, and induce caspase-3 expression, which may trigger apoptosis.
Wang
Wei
W
Department of Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Wang
Ping-fang
PF
Sheng
Zhi-feng
ZF
Fang
Jian-zheng
JZ
Liu
Feng-ying
FY
D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects*; D000924:Anticholesteremic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis* / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D009852:Omentum / Q000166:cytology; D019821:Simvastatin / Q000494:pharmacology*
D000924:Anticholesteremic Agents; D019821:Simvastatin; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases