title: The relationship between CYP17 -34T/C polymorphism and acne in Chinese subjects revealed by sequencing.
abstract: BACKGROUND
  Although many arguments have been raised on the role of heredity in the etiology of acne, the relevant genetic elements in the pathogenesis of the disease are not well established.
  
  
  OBJECTIVE
  The aim of our study was to evaluate the association between a genetic polymorphism in the promoter region of the CYP17 gene and the development of acne.
  
  
  METHODS
  206 acne patients and 200 controls were included in the study. A polymerase chain reaction (PCR) sequencing technique was used to reveal a CYP17 gene polymorphism in its promoter region. A chi2 test was used for data analysis.
  
  
  RESULTS
  CYP17 -34T/C polymorphism was found and the frequency distribution of the C/C homozygotes and C allele in the male patients with severe acne (33.3 and 60.9%, respectively) were statistically significantly different from those of the control samples (18.2 and 46.6%; p < 0.05). No significant difference was observed between the female patients, mild + moderate male patients and their controls, respectively.
  
  
  CONCLUSIONS
  The CYP17 -34C/C homozygote Chinese men are at a significantly increased risk of developing severe acne.
authors.affiliation: Key Laboratory of Bioresources Conservation and Utilization and Human Genetics Center of Yunnan University, Kunming, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yang
authors.forename: Haijing
authors.initials: H
authors.lastname: Yu
authors.forename: Baoweng
authors.initials: B
authors.lastname: Cheng
authors.forename: Wenru
authors.initials: W
authors.lastname: Tang
authors.forename: Yongli
authors.initials: Y
authors.lastname: Dong
authors.forename: Chunjie
authors.initials: C
authors.lastname: Xiao
mesh_terms: D000152:Acne Vulgaris / Q000201:enzymology / Q000235:genetics*; D000465:Algorithms; D016022:Case-Control Studies; D002681:China; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics*
chemical_list: D013254:Steroid 17-alpha-Hydroxylase