The detection of sedatives in hair and nail samples using tandem LC-MS-MS.
Drug screening methods were developed to detect alprazolam, clobazam, clonazepam, diazepam, midazolam, oxazepam, temazepam, triazolam, zopiclone, and selected metabolites in human hair and nail samples employing liquid-liquid extraction and tandem liquid chromatography-mass spectrometry (LC-MS-MS). Hair and nail samples were obtained from patients who had recently discontinued or were currently prescribed one or more of the targeted drugs. Prazepam was used as the internal standard for all compounds. Some components in the hair matrix gave the same transitions as some of the analytes but did not compromise the analyses because their retention times differed from those for the target compounds. The analytical run time was 8-10min. Results of the hair analysis of a DFSA victim are also presented.
Irving
Rachel C
RC
Department of Chemistry, The University of Waikato, Knighton Road, Hamilton, New Zealand.
Dickson
Stuart J
SJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000525:Alprazolam / Q000032:analysis; D053961:Azabicyclo Compounds; D001569:Benzodiazepines / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D000078306:Clobazam; D002998:Clonazepam / Q000032:analysis; D003975:Diazepam / Q000032:analysis; D005260:Female; D049429:Forensic Pathology; D006197:Hair / Q000737:chemistry*; D006801:Humans; D006993:Hypnotics and Sedatives / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008874:Midazolam / Q000032:analysis; D008875:Middle Aged; D009262:Nails / Q000737:chemistry*; D010076:Oxazepam / Q000032:analysis; D010879:Piperazines / Q000378:metabolism; D011237:Predictive Value of Tests; D011902:Rape; D013693:Temazepam / Q000032:analysis; D014229:Triazolam / Q000032:analysis
D053961:Azabicyclo Compounds; D006993:Hypnotics and Sedatives; D010879:Piperazines; C515050:zopiclone; D001569:Benzodiazepines; D014229:Triazolam; D000078306:Clobazam; D002998:Clonazepam; D010076:Oxazepam; D013693:Temazepam; D003975:Diazepam; D008874:Midazolam; D000525:Alprazolam