Secretory factors of human neuroblastoma (IMR-32) and human glioblastoma (U87MG) cell lines induce neurite outgrowths in PC12 cells.
Neurite outgrowth is essential for the communication of the nervous system. The rat Pheochromocytoma (PC12) cells are commonly used in the neuronal cell study. It is well known that exogenous stimuli such as Nerve Growth Factor (NGF) induce neurite outgrowth. In the present study it has been investigated whether or not the conditioned medium from human neuroblastoma cell line (IMR-32) and human glioblastoma cell line (U87MG) may augment neurite outgrowth in PC12 cells. PC12 were cultured with and without conditioned media of IMR-32 and U87MG. The result showed that both the conditioned media induce neurite outgrowth within 48 hr and stops further proliferation of PC12 cells. However no outgrowth was noted in PC12 cells incubated without conditioned medium. In conclusion, it is shown that both the conditioned media (IMR-32 and U87MG) have the potential to induce the neurite outgrowth in the PC12 cells.
Satpute
Ravindra M
RM
Biochemistry Research Laboratory, Central India Institute of Medical Sciences, 88/2, Bajaj Nagar, Nagpur 440 010, India.
Kashyap
Rajpal S
RS
Kainthla
Rani Poonam
RP
Purohit
Hemant J
HJ
Taori
Girdhar M
GM
Daginawala
Hatim F
HF
D000818:Animals; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D016501:Neurites; D009447:Neuroblastoma / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats
D017077:Culture Media, Conditioned