Degradation of PrP(Sc) by keratinolytic protease from Nocardiopsis sp. TOA-1.
A keratinolytic alkaline proteae (NAPase) from Nocardiopsis sp. TOA-1 degraded a scrapie prion without any chemical or physical treatment. Optimal temperature and pH were 60 degrees C and above pH 10.0. The scrapie prion was completely degraded within 3 min under optimal conditions.
Mitsuiki
Shinji
S
Department of Applied Chemistry and Biochemistry, Kyushu Sangyo University, Fukuoka, Japan.
Hui
Zhao
Z
Matsumoto
Daishi
D
Sakai
Masashi
M
Moriyama
Yasushi
Y
Furukawa
Kensuke
K
Kanouchi
Hiroaki
H
Oka
Tatsuzo
T
D000192:Actinomycetales / Q000201:enzymology*; D000818:Animals; D001673:Biodegradation, Environmental; D006224:Cricetinae; D006863:Hydrogen-Ion Concentration; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism*; D018620:PrPSc Proteins / Q000378:metabolism*; D013696:Temperature
D018620:PrPSc Proteins; D010447:Peptide Hydrolases; C035855:keratinase