Human rotavirus serotype G9, São Paulo, Brazil, 1996-2003.
A total of 3,101 fecal specimens were collected during an 8-year survey for rotavirus infection in São Paulo, Brazil. Group A rotavirus was detected in 774 (25.0%) specimens. Of these, 431 strains (55.7%) were analyzed for G and P types by reverse transcription-polymerase chain reaction; G1 was the predominant serotype (68.2%), followed by G9 (17.2%), G4 (6.3%), G2 (1.2%), G3 (0.7%), mixed infection (1.8%), and untypeable (4.6%). Both rotavirus G and P types could be established in 332 strains (77.0%). We identified the 4 most common strains worldwide: P[8]G1 (66.6%), P[4]G2 (1.0%), P[8]G3 (0.6%), and P[8]G4 (7.2%). Among the single G9 strains detected, VP4 genotyping showed that P[8]G9 was the most prevalent, followed by P[4]G9 and P[6]G9. The emergence and high frequency of rotavirus G9 in São Paulo, Brazil, and other parts of the world will affect the development and evaluation of future vaccines.
Carmona
Rita Cássia Compagnoli
RC
Laboratório de Vírus Entéricos, Adolfo Lutz Institute, São Paulo, Brazil. rcarmona@ial.sp.gov.br
Timenetsky
Maria do Carmo Sampaio Tavares
Mdo C
Morillo
Simone Guadagnucci
SG
Richtzenhain
Leonardo José
LJ
D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D006801:Humans; D008875:Middle Aged; D015995:Prevalence; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D012703:Serotyping
D036022:Capsid Proteins; D012367:RNA, Viral