title: Successful 40-hour preservation of the canine small intestine with the cavitary 2-layer method with glutamine supplementation.
abstract: BACKGROUND
  We recently reported that the cavitary 2-layer method (cTLM) allowed stable 24-hour preservation of canine intestine. The aim of this study was to examine the possibility of the 40-hour preservation by cTLM that is supplemented with glutamine.
  
  
  METHODS
  Canine jejunal segments (40 cm) were allotransplanted heterotopically without storage (group 1), after 40-hour cold storage with University of Wisconsin solution (group 2), cTLM (group 3), UW with 2% glutamine (group 4), or cTLM with 2% glutamine (group 5). Mucosal glutamine, histidine, tryptophan, glutathione, and adenosine triphosphate concentrations were determined immediately after preservation. At day 7 after the transplantation, maltose absorption test and histopathologic analysis were performed.
  
  
  RESULTS
  Mucosal glutamine concentrations increased significantly with glutamine supplementation during preservation in groups 4 and 5 (P < .01). Mucosal adenosine triphosphate levels in cTLM groups (groups 3 and 5) were similar to those in group 1; group 4 showed very low levels after preservation, despite glutamine supplementation. Mucosal glutathione did not differ among groups 2, 3, 4, and 5 and were significantly lower than in group 1. In the absorption test, serum glucose curves showed a peak level by 30 minutes in groups 1 and 5; delayed peaks were seen in groups 2, 3, and 4 compared with group 1. The villous heights were 879, 555, 685, 688, and 773 microm in groups 1, 2, 3, 4, and 5, respectively (group 1 vs group 2, 3, and 4: P < .05).
  
  
  CONCLUSIONS
  cTLM that was supplemented with glutamine extended a safe preservation period up to 40 hours in canine segmental small intestinal transplantation, although this study failed to elucidate a precise mechanism of the glutamine beneficial effect on the graft mucosa.
authors.affiliation: Department of Gastroenterological Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Shiri
authors.initials: S
authors.lastname: Li
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fujino
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Kakinoki
authors.forename: Takuro
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Naohiro
authors.initials: N
authors.lastname: Goto
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakai
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kuroda
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004285:Dogs; D005260:Female; D005973:Glutamine / Q000494:pharmacology*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000187:drug effects / Q000502:physiology; D007421:Intestine, Small / Q000187:drug effects / Q000502:physiology* / Q000637:transplantation*; D007700:Kinetics; D008297:Male; D008320:Maltose / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D005973:Glutamine; D000244:Adenosine Diphosphate; D008320:Maltose