title: (-)Schisandrin B is more potent than its enantiomer in enhancing cellular glutathione and heat shock protein production as well as protecting against oxidant injury in H9c2 cardiomyocytes.
abstract: Effects of schisandrin B enantiomers ((+)Sch B and (-)Sch B) treatment on cellular reduced glutathione (GSH) level and heat shock protein (Hsp)25/70 production were investigated in H9c2 cardiomyocytes. (+)Sch B and (-)Sch B at 6.25 muM produced a time-dependent and biphasic change in cellular GSH level and Hsp25/70 production, with the stimulatory effect of (-)Sch B being more potent. The GSH- and Hsp-enhancing effects were accompanied by a parallel cytoprotection against xanthine oxidase/xanthine-induced toxicity, with the biphasic time course of (+)Sch B- or (-)Sch B-induced protection being superimposed with that of the increase in GSH level but not Hsp25/70 production. The results indicate that (-)Sch B produces more potent enhancing effects on cellular GSH and Hsp production as well as protection against oxidative injury than (+)Sch B in cardiomyocytes.
authors.affiliation: Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
authors.forename: Po Yee
authors.initials: PY
authors.lastname: Chiu
authors.forename: Hoi Yan
authors.initials: HY
authors.lastname: Leung
authors.forename: Michel K T
authors.initials: MK
authors.lastname: Poon
authors.forename: Duncan H F
authors.initials: DH
authors.lastname: Mak
authors.forename: Kam Ming
authors.initials: KM
authors.lastname: Ko
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D034242:Cyclooctanes / Q000737:chemistry / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D017705:Lignans / Q000737:chemistry* / Q000494:pharmacology*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016877:Oxidants / Q000378:metabolism*; D011083:Polycyclic Compounds / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D013237:Stereoisomerism; D013997:Time Factors; D019820:Xanthine / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D034242:Cyclooctanes; D018840:HSP70 Heat-Shock Proteins; D017705:Lignans; D016877:Oxidants; D011083:Polycyclic Compounds; C015499:schizandrin B; D019820:Xanthine; D014969:Xanthine Oxidase; D005978:Glutathione