title: Reproducibility of MDM2 and CDK4 staining in soft tissue tumors.
abstract: MDM2 and CDK4 immunostaining can be useful adjuncts in diagnosing liposarcoma among soft tissue neoplasms. We examined the reproducibility of MDM2 and CDK4 staining between 2 laboratories and between tissue microarrays and whole tissue sections. Sixty-two soft tissue tumors were immunostained at the Bergonié Institute, Bordeaux, France, and the Curie Institute, Paris, France. We also examined 203 soft tissue neoplasms on standard tissue sections and tissue microarrays. There was high concordance of results obtained from the 2 laboratories (with 2 different pathologists) for MDM2 (kappa, 0.93) and CDK4 (kappa, 0.8) staining. There also was excellent concordance between results on tissue microarray and on whole tissue sections for MDM2 (kappa, 0.80) and CDK4 (kappa, 0.93). Immunostaining for MDM2 and CDK4 is a reproducible technique that may be exported to different laboratories for routine use. Tissue microarray is indicated for studying large series.
authors.affiliation: Department of Pathology, Bergonié Institute and University Victor Segalen, 229 Cours de l'Argonne, 33076 Bordeaux Cédex, France.
authors.forename: Matthieu Bui Nguyen
authors.initials: MB
authors.lastname: Binh
authors.forename: Xavier Sastre
authors.initials: XS
authors.lastname: Garau
authors.forename: Louis
authors.initials: L
authors.lastname: Guillou
authors.forename: Alain
authors.initials: A
authors.lastname: Aurias
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Coindre
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis*; D015588:Observer Variation; D051736:Proto-Oncogene Proteins c-mdm2 / Q000032:analysis*; D015203:Reproducibility of Results; D012189:Retrospective Studies; D012509:Sarcoma / Q000737:chemistry* / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000737:chemistry* / Q000473:pathology*; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D007633:Keratins; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2