title: Hydroxyapatite/SiO(2)-CaO-P(2)O(5) glass materials: in vitro bioactivity and biocompatibility.
abstract: Materials obtained by the heat treatment of mixtures of hydroxyapatite (HA) and a silicate-based glass of the system SiO(2)-CaO-P(2)O(5) have been investigated. The influence of the glass content on the porosity, microstructure and on the constituent phases of the final materials was studied. The influence of these factors on the in vitro bioactive behaviour of the obtained materials was also investigated. In addition, an in vitro biocompatibility assay with osteoblastic-like cells was carried out. The addition of the glass to HA induced different solid-state reactions that yield the transformation of HA into alpha- and beta-tricalcium phosphate as well as the formation of silicon-containing phases (silicocarnotite or pseudowollastonite). In these mixtures an enhancement in the porosity, pore size and a heterogeneous microstructure was observed, compared with the precursors. As the sol gel glass content increased, the previous effects were higher. The materials showed the formation of an apatite-like layer on their surface when soaked in simulated body fluid, being faster in the sample with a higher content of glass. The formation of the new layer began in preferential zones in both samples, depending on the different reactivity of the crystalline phases formed. A synergistic effect between HA and glass was observed, showing in the mixtures a faster bioactive behaviour than in HA and glass themselves. The obtained materials allow a good attachment, spread and proliferation of the osteoblastic-like cells and no cytotoxic effect was observed.
authors.affiliation: Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Padilla
authors.forename: J
authors.initials: J
authors.lastname: Román
authors.forename: S
authors.initials: S
authors.lastname: Sánchez-Salcedo
authors.forename: M
authors.initials: M
authors.lastname: Vallet-Regí
mesh_terms: D001672:Biocompatible Materials; D001826:Body Fluids; D017610:Calcium Compounds; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003460:Crystallization; D003461:Crystallography; D017886:Durapatite; D005898:Glass / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010087:Oxides; D010100:Oxygen; D010758:Phosphorus; D016062:Porosity; D012825:Silicon; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D020099:Coated Materials, Biocompatible; D010087:Oxides; D010758:Phosphorus; D017886:Durapatite; C016538:lime; D010100:Oxygen; D012825:Silicon