Nepsilon-thioacetyl-lysine: a multi-facet functional probe for enzymatic protein lysine Nepsilon-deacetylation.
Peptides containing Nepsilon-thioacetyl-lysine and Nepsilon-acetyl-lysine were evaluated for their de(thio)acetylation catalyzed by human HDAC8 and SIRT1, two distinct protein deacetylases. Nepsilon-Thioacetyl-lysine was found to be a mimic of Nepsilon-acetyl-lysine for HDAC8-catalyzed reaction, but to confer inhibition against SIRT1. These results point to further diverse applications based on Nepsilon-thioacetyl-lysine.
Fatkins
David G
DG
Department of Chemistry, University of Akron, 190 E. Buchtel Commons, Akron, OH 44325, USA.
Monnot
Andrew D
AD
Zheng
Weiping
W
D000107:Acetylation; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D006655:Histone Deacetylases / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D018716:Molecular Mimicry; D010455:Peptides / Q000737:chemistry; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism; D056564:Sirtuin 1; D037761:Sirtuins / Q000737:chemistry / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry
C513649:N(epsilon)-thioacetyllysine; D010455:Peptides; D012097:Repressor Proteins; D013438:Sulfhydryl Compounds; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D037761:Sirtuins; C409470:HDAC8 protein, human; D006655:Histone Deacetylases; D008239:Lysine