title: Preparation of optically pure tert-leucine by penicillin G acylase-catalyzed resolution.
abstract: Penicillin G acylase, from Kluyvera citrophila, was used in kinetic resolution of DL-tert-leucine. N-phenylacetylated-DL-tert-leucine, chemically synthesized from DL-tert-leucine, was enantioselctively hydrolyzed by penicillin G acylase to obtain L-tert-leucine, D-tert-leucine was prepared by acid-catalyzed hydrolysis of the remaining substrate. The total yields of D-tert-leucine and L-tert-leucine are 80.6% and 83.1%, respectively. The enantiomeric excess of the two products, D-tert-leucine and L-tert-leucine, are 98.5% and 99%. This is a practical way for the preparation of D-tert-leucine and L-tert-leucine.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, P. R. China.
authors.forename: Shu-Lai
authors.initials: SL
authors.lastname: Liu
authors.forename: Qing-Xun
authors.initials: QX
authors.lastname: Song
authors.forename: Dong-Zhi
authors.initials: DZ
authors.lastname: Wei
authors.forename: Ye-Wang
authors.initials: YW
authors.lastname: Zhang
authors.forename: Xue-Dong
authors.initials: XD
authors.lastname: Wang
mesh_terms: D002384:Catalysis; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D044086:Kluyvera / Q000201:enzymology; D007930:Leucine / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009904:Optical Rotation; D010399:Penicillin Amidase / Q000378:metabolism*; D010400:Penicillin G / Q000378:metabolism
chemical_list: D010399:Penicillin Amidase; D007930:Leucine; D010400:Penicillin G