Flow injection analysis-Rayleigh light scattering detection for online determination of protein in human serum sample.
A flow injection analysis (FIA) system combined with Rayleigh light scattering (RLS) detection is developed for the sensitive and rapid determination of protein concentration in human serum sample. This method is based on the weak intensity of RLS of Eriochrome Black T (EBT, 2-hydroxy-1-(1-hydroxy-2-naphthylazo)-6-nitronaphthalene-4-sulfonic acid sodium salt), which can be enhanced by the addition of protein in weakly acidic solution. The effects of pH and interfering species on the determination of protein were examined. Calibrations for protein, based on RLS intensity, were linear in the concentration ranges of 7-36 microg/ml for human serum album (HSA) and 8-44 microg/ml for bovine serum album (BSA). The detection limits of the method were found to be 0.882 and 2.507 microg/ml for HSA and BSA, respectively. A relative standard deviation of 0.76% (n=5) was obtained with 20 microg/ml HSA standard solution. The FIA-RLS method was more stable than the general RLS method, and the average RSD value of FIA-RLS was less than that of the general RLS. The sample rate was determined to be 90 samples per hour.
Li
Ying
Y
Department of Chemistry, Lanzhou University, Lanzhou, Gansu Province 730000, China.
Dong
Lijun
L
Wang
Weiping
W
Hu
Zhide
Z
Chen
Xingguo
X
D000818:Animals; D001391:Azo Compounds / Q000737:chemistry; D001798:Blood Proteins / Q000032:analysis*; D002138:Calibration; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis
D001391:Azo Compounds; D001798:Blood Proteins; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; C014527:Eriochrome Black T