Automated separation of cord blood units in top and bottom bags using the Compomat G4.
Cord blood (CB) has become a real alternative source of haematopoietic stem cells for bone marrow reconstitution in a variety of malignant disorders. As a response to this increasing activity, CB banks have been developed to guarantee the quality of processed CB units. Volume reduction of CB units maximizes storage space and also has other advantages. The aim of this study was to develop a program for the volume reduction of CB in the Compomat G4 device. We also compared two different top and bottom systems for CB fractionation (Compomat G4 and Optipress II). We empirically designed three different programs for volume reduction of CB with Compomat G4: two for final BC volume of 41 ml (CB1 and CB2) and the other one for buffy coat (BC) volume of 25 ml (CB3). Significantly worse recoveries were achieved for CB processed with program CB3. A RBC depletion of >or=50%, >or=60% and >or=70% were achieved for 67%, 39% and 9% of all units respectively. When comparing Compomat G4 and Optipress II, total nucleated cell recovery was similar for both methods, while lymphocytes recovery was significantly better for Optipress II.
Solves
P
P
Umbilical Cord Blood Bank, Valencia Transfusion Centre, Valencia, Spain. solves_pil@gva.es
Mirabet
V
V
Carbonell-Uberos
F
F
Soler
M A
MA
Roig
R
R
D018952:Antigens, CD34 / Q000032:analysis; D000094902:Blood Banking / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D015925:Cryopreservation / Q000379:methods; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D015397:Program Evaluation; D018709:Statistics, Nonparametric; D013234:Stem Cells / Q000166:cytology*
D018952:Antigens, CD34