Determination of cellular localization of expression of flavin-containing monooxygenase genes in mouse tissues by in situ hybridization.
Methods are described for the cellular localization of expression of flavin-containing monooxygenase (FMO) genes in various mouse tissues by in situ hybridization. These include the production of digoxigenin (DIG)-labeled antisense and sense RNA probes by transcription from FMO cDNA templates, the preparation of paraffin wax-embedded and cryostat tissue sections, the hybridization of RNA probes to tissue sections, and the specific detection of hybridized probes using an antibody to DIG.
Janmohamed
Azara
A
Department of Biochemistry and Molecular Biology, University College London, UK.
Phillips
lan R
lR
Shephard
Elizabeth A
EA
D000818:Animals; D001921:Brain / Q000201:enzymology; D018076:DNA, Complementary; D015870:Gene Expression; D017403:In Situ Hybridization; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D051379:Mice; D010105:Oxygenases / Q000235:genetics*
D018076:DNA, Complementary; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming)