title: Circulating dendritic cells subsets and regulatory T-cells at multiple sclerosis relapse: differential short-term changes on corticosteroids therapy.
abstract: Glucocorticoids remain the treatment of choice for MS relapses. However, little is known on the effect of intravenous methylprednisolone (IVMP) on dendritic cells (DCs) and regulatory T-cells (TReg). Our main goal was to quantify circulating myeloid and plasmacytoid DCs (mDCs and pDCs), and TReg at MS relapse versus healthy controls; and to analyse the short-term changes after IVMP for MS relapse. MS patients at relapse compared to controls showed higher %CD4+CD25high+ TReg (p<0.01). After 5-days of IVMP, activated T-lymphocytes (p=0.001), pDCs (p<0.0001), and CD11c+ mDCs (p<0.0001) decreased. By contrast, CD4+CD25+ and CD4+CD25high+ TReg further increased (p<0.0001 both). Changes on these subsets may play a relevant role in the immunosuppressive activity of this drug.
authors.affiliation: Department of Immunology, Gregorio Marañón University General Hospital, Madrid, Spain.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Navarro
authors.forename: Carol
authors.initials: C
authors.lastname: Aristimuño
authors.forename: Silvia
authors.initials: S
authors.lastname: Sánchez-Ramón
authors.forename: Dolores
authors.initials: D
authors.lastname: Vigil
authors.forename: Ma Luisa
authors.initials: ML
authors.lastname: Martínez-Ginés
authors.forename: Eduardo
authors.initials: E
authors.lastname: Fernández-Cruz
authors.forename: Clara
authors.initials: C
authors.lastname: de Andrés
mesh_terms: D000293:Adolescent; D000328:Adult; D003713:Dendritic Cells / Q000187:drug effects*; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007275:Injections, Intravenous; D008213:Lymphocyte Activation; D008297:Male; D008775:Methylprednisolone / Q000008:administration & dosage*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*
chemical_list: D006684:HLA-DR Antigens; D008775:Methylprednisolone