[Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC].
OBJECTIVE
To determine 20(S)-ginsengnoside Rh2 in the hydrolysis product of saponins from leaves of Panax qinquefolium.


METHODS
The separation was performed on ZORBAX EXEND C18 column (4.6 mm x 250 mm, 5 microm), eluted with methanol and water (85:15) as mobile phase with the rate of 1.2 mL x min(-1) at 25 degrees C, the wavelength for measurement was 203 nm.


RESULTS
The calibration curve was linear in the range of 0.5-25 microg for 20(S)-ginsengnoside Rh2(r = 0.9999, n = 7). The average recovery was 99.7% (RSD= 1.0%).


CONCLUSIONS
This method is simple, accurate, reliable and reproducible. The result shows that the transform ratio of 20(S)-ginsengnoside Rh2 is high by this hydrolysis method.
Li
Xu-wen
XW
College of Chemistry, Jilin Unirersity, Changchun, China. xwli@jlu.edu.cn
Gui
Ming-yu
MY
Zheng
Ying
Y
Jin
Yong-ri
YR
Zhang
Han-qi
HQ
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D036145:Ginsenosides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D005894:Panax / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D036145:Ginsenosides; C055305:ginsenoside Rh2