New synthetic route for RGD tripeptide.
A new route was employed to synthesize RGD. First, Gly-Asp dipeptide was synthesized by a novel chemical method in two steps, including chloroacetylation of L-aspartic acid and ammonolysis of chloroacetyl L-aspartic acid. Second, Nalpha-Z- L-Arginine was reacted with Gly-Asp to synthesize RGD by the N-carboxyanhydride method. Less protected amino acids were used in this synthesis. This method possessed advantages of low cost, simplicity, and rapidity with a reasonable yield of 62% calculated from arginine. In addition, compared with the above method, a conventional solid phase method was also used to synthesize RGD, the yield was 75% calculated from the first amino acid anchored to resin.
Hou
Rui-Zhen
RZ
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, P.R. China.
Liu
Yun-Jia
YJ
Zhang
Na
N
Huang
Yi-Bing
YB
Wang
Hua
H
Yang
Yan
Y
Xu
Li
L
Zhang
Xue-Zhong
XZ
D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry
D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid