title: The effect of recombinant human intrinsic factor on the uptake of vitamin B12 in patients with evident vitamin B12 deficiency.
abstract: We report on the use of recombinant human intrinsic factor (rhIF) in a new vitamin B12 absorption test. Holotranscobalamin (holoTC) was measured before and 24 hours after intake of three 9-mg doses of vitamin B12 (B12) and again 24 hours after intake of the same dose of B12 together with rhIF (rhIF-B12). Nine patients with evident vitamin B12 deficiency had a significantly higher increase in holoTC after intake of rhIF-B12 than after intake of B12. Twenty-eight patients with suspected vitamin B12 deficiency showed no additional increase in holoTC after intake of rhIF-B12. We conclude that rhIF promotes B12 absorption among patients with evident vitamin B12 deficiency.
authors.affiliation: Department of Clinical Biochemistry, Skejby Hospital and Aarhus Hospital, Aarhus University Hospital, Aarhus, Denmark. am.hvas@dadlnet.dk
authors.forename: Anne-Mette
authors.initials: AM
authors.lastname: Hvas
authors.forename: Hora
authors.initials: H
authors.lastname: Buhl
authors.forename: Niels Bech
authors.initials: NB
authors.lastname: Laursen
authors.forename: Birger
authors.initials: B
authors.lastname: Hesse
authors.forename: Lars
authors.initials: L
authors.lastname: Berglund
authors.forename: Ebba
authors.initials: E
authors.lastname: Nexo
mesh_terms: D001692:Biological Transport; D006801:Humans; D007408:Intestinal Absorption; D007437:Intrinsic Factor / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000627:therapeutic use; D014805:Vitamin B 12 / Q000493:pharmacokinetics* / Q000627:therapeutic use; D014806:Vitamin B 12 Deficiency / Q000188:drug therapy*
chemical_list: D011994:Recombinant Proteins; D007437:Intrinsic Factor; D014805:Vitamin B 12