title: Development of beta-tricalcium phosphate/collagen sponge composite for bone regeneration.
abstract: Synthetic biomaterials have been developed and used for bone grafting. Here, we developed a biodegradable sponge composite for bone tissue engineering by combining beta-tricalcium phosphate (beta-TCP) and collagen. In addition, we sought to determine the optimal beta-TCP granules/collagen ratio by evaluating and bone formation in vivo. Porous beta-TCP granules were mixed with atelocollagen hydrochloride solution at various ratios--0.02, 0.05, 0.1, and 0.2 g/mL. The resultant mixtures were freeze-dried and subjected to dehydrothermal treatment in vacuo. The final composites obtained were designated beta-TCP/collagen sponge composites (beta-TCP/CS). Through compression testing, it was found that the stress values for beta-TCP/CS (0.2 g/mL) were higher than those of the other three composites over the whole strain range. Histological evaluation at four weeks after implantation revealed that the collagen sponge had degraded and newly formed bone was present on the surface of the beta-TCP granules. At 12 weeks, the beta-TCP granules were completely degraded and remodeling of the lamellar bone was observed.
authors.affiliation: Department of Oral & Maxillofacial Surgery, The Nippon Dental University School of Dentistry at Tokyo, Japan. matsunot@tky.ndu.ac.jp
authors.forename: Tomonori
authors.initials: T
authors.lastname: Matsuno
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Koh-ichi
authors.initials: K
authors.lastname: Kuremoto
authors.forename: Syunsuke
authors.initials: S
authors.lastname: Notazawa
authors.forename: Taka
authors.initials: T
authors.lastname: Nakahara
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Tazuko
authors.initials: T
authors.lastname: Satoh
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry* / Q000627:therapeutic use; D002130:Calcium Phosphates / Q000737:chemistry* / Q000627:therapeutic use; D003094:Collagen / Q000737:chemistry* / Q000627:therapeutic use; D019245:Compressive Strength; D004285:Dogs; D008422:Materials Testing; D013552:Swine
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D003094:Collagen