Enantiomeric separation of fluoxetine derivatives on polysaccharide-based chiral columns.
A high performance liquid chromatography (HPLC) method employing amylose-based chiral columns (Chiralpak AD-RH and Chiralpak AD) and cellulose-based chiral columns (Chiralcel OD) as chiral stationary phases have been developed for the enantiomeric separation of fluoxetine (FLX) derivatives. The FLX was derivatized with 4-(N-chloroformylmethyl-N-methyl)amino-7-nitro-2,1,3-benzoxadiazole (NBD-COCl) and 4-(N-chloroformylmethyl-N-methyl)amino-7-N,N-dimethylaminosulfonyl-2,1,3-benzoxadiazole (DBD-COCl), respectively. Influence of the mobile phase composition and column temperature on the enantioseparation was discussed during the separation. On the basis of separation of derivatized FLX enantiomers, the paper also discussed the separation mechanism on the chiral stationary phases used.
Yu
Li
L
Department of Analytical Chemistry, School of Pharmacy, Shenyang Pharmaceutical University, China.
Mei-Li
Fa
F
Jie-Guo
Xing
X
D019840:2-Propanol / Q000737:chemistry; D000688:Amylose / Q000031:analogs & derivatives* / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D005453:Fluorescence; D005473:Fluoxetine / Q000737:chemistry* / Q000302:isolation & purification; D006860:Hydrogen Bonding; D015394:Molecular Structure; D048448:Phenylcarbamates / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D017367:Selective Serotonin Reuptake Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D013696:Temperature
D019840:2-Propanol; D000688:Amylose; D002482:Cellulose; D005473:Fluoxetine; D048448:Phenylcarbamates; D011134:Polysaccharides; D017367:Selective Serotonin Reuptake Inhibitors; D012997:Solvents; C080033:Chiralpak AD