Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A.
The diversity of polyketide synthase (PKS) genes in Aspergillus ochraceus NRRL 3174 and Aspergillus carbonarius 2Mu134 has been investigated using different primer pairs previously developed for the ketosynthase (KS) domain of fungal PKSs. Nine different KS domain sequences in A. ochraceus NRRL 3174 as well as five different KS domain sequences in A. carbonarius 2Mu134 have been identified. The identified KS fragments were distributed in five different clusters on the phylogenetic tree, indicating that they most probably represent PKSs responsible for different functions.
Atoui
Ali
A
Département Bioprocédés et Systèmes Microbiens, Laboratoire de Génie Chimique UMR5503 (CNRS/INPT/UPS), Ecole Nationale Supérieure Agronomique de Toulouse, Institut National Polytechnique de Toulouse, France.
Dao
Huy Phong
HP
Mathieu
Florence
F
Lebrihi
Ahmed
A
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D001235:Aspergillus ochraceus / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010398:Penicillic Acid / Q000378:metabolism; D010802:Phylogeny; D048630:Polyketide Synthases / Q000737:chemistry* / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial; D048630:Polyketide Synthases; D010398:Penicillic Acid