Effect of sulfide on the immune response and susceptibility to Vibrio alginolyticus in the kuruma shrimp Marsupenaeus japonicus.
Kuruma shrimp Marsupenaeus japonicus held in 34 per thousand seawater were injected with tryptic soy broth (TSB)-grown Vibrio alginolyticus (2.7x10(6)cfu shrimp(-1)), and then placed in water containing concentrations of sulfide at 0 (control), 51, 106, 528 and 1050microgl(-1), respectively. After 12-144h, mortality of V. alginolyticus-injected shrimp exposed to 528 and 1102microgl(-1) sulfide was significantly higher than that of shrimp exposed to 51microgl(-1) sulfide and the control solution. In another experiment, M. japonicus which had been exposed to 0, 56, 112, 525 and 1076microgl(-1) sulfide for 6, 12, 24 and 48h were examined for immune parameters, and phagocytic activity and clearance efficiency of V. alginolyticus. Sulfide concentrations at 525microgl(-1) or greater for 12h resulted in decreased total haemocyte count (THC) and phenoloxidase activity, phagocytic activity and bacterial clearance efficiency, whereas a sulfide concentration at 1076microgl(-1) for 24h caused a significant increase in respiratory burst and superoxide dismutase activity of M. japonicus. It is concluded that concentrations of sulfide at 528microgl(-1) or greater increased the susceptibility of M. japonicus against V. alginolyticus infection by a depression in immune ability. The increased production of superoxide anion by M. japonicus exposed to 525microgl(-1) sulfide or greater was considered to be cytotoxic to the host.
Cheng
Sha-Yen
SY
Department of Environmental Biology and Fisheries, National Taiwan Ocean University, Keelung, Taiwan 202, ROC.
Hsu
Si-Wei
SW
Chen
Jiann-Chu
JC
D000818:Animals; D004781:Environmental Exposure; D006434:Hemocytes / Q000187:drug effects; D014442:Monophenol Monooxygenase / Q000187:drug effects / Q000378:metabolism; D033561:Penaeidae / Q000187:drug effects* / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects; D016897:Respiratory Burst / Q000187:drug effects; D013440:Sulfides / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000187:drug effects; D013997:Time Factors; D044149:Vibrio alginolyticus / Q000276:immunology* / Q000472:pathogenicity
D013440:Sulfides; D014442:Monophenol Monooxygenase; D013482:Superoxide Dismutase