title: [Construction of two hepatocellular carcinoma cell models for the expression of HBV X gene with different selection characteristics].
abstract: OBJECTIVE
  To construct 2 hepatocellular carcinoma (HCC) cell models for the expression of HBV X gene with different selection characteristics.
  
  
  METHODS
  HepG2 HCC cells were infected with eukaryotic expression vectors with HBV X gene, pCEP4-X, and pcDNA3. 1 (+)-X. Single cell clone was selected by hygromycin and neomycin. After propagating culture for certain periods, the HBV X gene expression was identified by PCR, RT-PCR, and Western blot.
  
  
  RESULTS
  Single HCC cell clone with HBV X gene transferred resistant to hygromycin and neomycin was selectively cultured, and the cells could be propagated for certain periods. PCR, RT-PCR, and Western blot identified the expression of HBV X gene.
  
  
  CONCLUSIONS
  Two HCC cell models for the expression of HBV X gene with different selection characteristics have been successfully constructed.
authors.affiliation: Liver Disease Research Center, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Xing-e
authors.initials: XE
authors.lastname: He
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Lei
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.forename: Wen-long
authors.initials: WL
authors.lastname: Wang
authors.forename: Hong-yu
authors.initials: HY
authors.lastname: Luo
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D005057:Eukaryotic Cells / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008954:Models, Biological; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein