title: Determination of the big head carp myofibrillar (Aristichthys nobilis) adenosine triphosphatase activity by ion chromatography.
abstract: A method for the determination of adenosine triphosphatase (ATPase) activity of myofibrils of big head carp by using ion chromatography was introduced. Adenosine triphosphate (ATP), adenosine diphosphate (ADP) and orthophosphate (Pi) were separated completely. Recoveries for ATP, ADP and Pi were 98+/-5%, 97+/-4% and 98+/-5%, respectively. Pi liberated from ATP during reaction was monitored by ion chromatography using the suggested method. This method was applicable to the determination of myofibrils ATPase activity for quick quality evaluation of surimi.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China.
authors.forename: Rui-chang
authors.initials: RC
authors.lastname: Gao
authors.forename: Chang-hu
authors.initials: CH
authors.lastname: Xue
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.forename: Zhao-jie
authors.initials: ZJ
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Xue
authors.forename: Fengxia
authors.initials: F
authors.lastname: Cui
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002138:Calibration; D002347:Carps; D002853:Chromatography, Liquid / Q000379:methods*; D006868:Hydrolysis; D015203:Reproducibility of Results
chemical_list: D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases