[Isolation and determination of silver-resistant bacteria plasmids].
Through the enrichment of the active mud obtained from three chemical plants and the domestication with different concentration Ag+ solution, thirty bacteria strains with silver (Ag+)-resistance were isolated, among which, the highest Ag+ -resistant concentration was 80 mg x ml(-1). The plasmids in these bacteria were extracted, with the detection rate of 76.67%. The elimination rate of the plasmid in HAg4 bacteria was 98.75% by 40 mmol x L(-1) sodium benzoate, and 77.78% by 350 microg x ml(-1) acridine orange. It was suggested that the Ag+ -resistance of bacteria was highly correlated with their plasmids.
Li
Junmin
J
Ecology Institute of Taizhou University, Linhai 317000, China. lijm@mail.tzptt.zj.cn
Jin
Zexin
Z
D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification*; D012722:Sewage / Q000382:microbiology; D012835:Silver Nitrate / Q000494:pharmacology*
D012722:Sewage; D012835:Silver Nitrate