title: Short-term retention of actin filament binding proteins on lamellipodial actin bundles.
abstract: Actin filaments are organised into sub-compartments of meshwork and bundles in lamellipodia. Localisation of fascin, the LIM and SH3 domain protein 1 (lasp-1), and lasp-2 to the bundles suggest their involvement in that organisation; however, their contributions remain unclear. We have compared the turnover of these proteins with actin at the bundle. After photobleaching, EGFP-actin recovered inwards from the bundle tip, consistent with the retrograde flow by treadmilling. In contrast, the recovery of EGFP-fascin, -lasp-1 and -lasp-2 occurred from the anterograde direction. These results suggest that these molecules would participate in the stabilisation of bundles but not in initiation.
authors.affiliation: Division of Biology, Faculty of Science, Fukuoka University, Fukuoka 814-0180, Japan. hnakagawa@fukuoka-u.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Asako G
authors.initials: AG
authors.lastname: Terasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Ohashi
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Miyamoto
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D060588:LIM Domain Proteins; D051379:Mice; D008840:Microfilament Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D038761:Photobleaching; D011554:Pseudopodia / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C096353:LASP1 protein, human; C484572:LDB2 protein, human; D060588:LIM Domain Proteins; D008840:Microfilament Proteins; D014157:Transcription Factors; C485184:enhanced green fluorescent protein; C020324:fascin; D049452:Green Fluorescent Proteins