Fresh PBSC harvests, but not BM, show temperature-related loss of CD34 viability during storage and transport.
BACKGROUND
The optimum conditions for storage and transport of freshly harvested HPC in the liquid state are uncertain. It is not specified in commonly applied standards for stem cell transplantation. We used a viable CD34 assay to determine the optimum temperature for maintaining progenitor cell viability in freshly harvested BM and PBSC. Our aim was to identify standardized conditions for storage and transport of marrow or peripheral blood products that would optimize CD34 recovery, leading to better transplant outcomes.


METHODS
Samples were aseptically removed from 46 fresh HPC harvests (34 PBSC and 12 BM) and stored at refrigerated temperature (2-8 degrees C), room temperature (18-24 degrees C) and 37 degrees C for up to 72 h. Samples were analyzed for viable CD34+ cells/microL at 0, 24, 48 and 72 h.


RESULTS
The mean viable CD34+ yield prior to storage was 7.7 x 10(6)/kg (range 0.7-30.3). The mean loss of viable CD34+ cells in HPC products at refrigerated temperature was 9.4%, 19.4% and 28% at 24, 48 and 72 h, respectively. In contrast, the mean loss of viable CD34+ cells at room temperature was 21.9%, 30.7% and 43.3% at 24, 48 and 72 h, respectively. No viable CD34+ cells remained after storage at 37 degrees C for 24 h. Only PBSC products and not BM showed temperature-related loss of CD34 viability. Greater loss of viable CD34+ cells was observed for allogeneic PBSC compared with autologous PBSC.


CONCLUSIONS
These results demonstrate that the optimum temperature for maintaining the viability of CD34+ cells, during overnight storage and transport of freshly harvested HPC, is 2-8 degrees C. These findings will allow the development of standard guidelines for HPC storage and transport.
Antonenas
V
V
Sydney Cellular Therapies Laboratory, Westmead Hospital, Sydney, New South Wales, Australia.
Garvin
F
F
Webb
M
M
Sartor
M
M
Bradstock
K F
KF
Gottlieb
D
D
D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002470:Cell Survival; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D036102:Peripheral Blood Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013696:Temperature; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D018952:Antigens, CD34