Diethylenetriaminepentaacetic acid-gadolinium (DTPA-Gd)-conjugated polysuccinimide derivatives as magnetic resonance imaging contrast agents.
Biocompatible polysuccinimide (PSI) derivatives conjugated with diethylenetriaminepentaacetic acid gadolinium (DTPA-Gd) were prepared as magnetic resonance imaging (MRI) contrast agents. In this study, we synthesized PSI derivatives incorporating methoxy-poly(ethylene glycol) (mPEG) as hydrophilic ligand, hexadecylamine as hydrophobic ligand, and DTPA-Gd as contrast agent. PSI was synthesized by the polycondensation polymerization of aspartic acid. All the synthesized materials were characterized by proton nuclear magnetic resonance (1H NMR). Critical micellization concentrations were determined using fluorescent probes (pyrene). Micelle size and shape were measured by electro-photometer light scattering (ELS) and atomic force microscopy (AFM). The formed micelle size ranged from 100 to 300 nm. The T1-weighted MR images of the phantom prepared with PSI-mPEG-C16-(DTPA-Gd) were obtained in a 3.0 T clinical MR imager, and the conjugates showed a great potential as MRI contrast agents.
Lee
Ha Young
HY
Department of Advanced Organic Materials Engineering, Chonbuk National University, Jeonju, Jeonbuk, Republic of Korea.
Jee
Hye Won
HW
Seo
Sung Mi
SM
Kwak
Byung Kook
BK
Khang
Gilson
G
Cho
Sun Hang
SH
D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D005682:Gadolinium / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D008823:Micelles; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D004369:Pentetic Acid / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*
D008823:Micelles; D010455:Peptides; C025252:poly-DL-succinimide; D001224:Aspartic Acid; D004369:Pentetic Acid; D005682:Gadolinium