Massive intestinal resection in rats fed up on glutamine: hepatic glycogen content valuation.
BACKGROUND
Glutamine has been widely used in treatment of small bowel syndrome and its metabolic effects on the small intestine are well known, however, it has been little studied its effects on hepatic metabolism under this condition.


OBJECTIVE
To verify through experimental model, a glutamine based supplemental diet, administered via oral to rats submitted to massive intestinal resection, evaluating weight evolution and hepatic glycogen content.


METHODS
Male rats, Wistar, were allocated into three groups to undergo enterectomy. Following diets were applied: with glutamine (G group), without glutamine (NG group), and standard diet from the laboratory (R group). All animals had massive small intestine resection including ileocecal valve removal. After 20 days, all animals were sacrificed. The liver was removed to histological analysis by light microscopy. Slides were stained by periodic acid of Schiff with diastasis.


RESULTS
All animals lost weight from the beginning to the end of experiment. Comparing weight loss average expressed in percentage, there was no difference statistically significant on this variance. In analyzed groups, the hepatic glycogen content did not differ statistically, in the histological method evaluated.


CONCLUSIONS
Glutamine feeding via oral did not influence weight loss reduction of animal submitted to massive intestinal resection and did not stimulate glycogen synthesis and storage into hepatocytes.
Miranda
Ariney Costa de
AC
Instituto de Assistência ao Servidor Público Estadual, University of São Paulo Medical School, São Paulo, SP, Brazil. ariney_costa@uol.com.br
Pinto
Paulo Engler
PE
Ribeiro
Sidney Resende
SR
Bromberg
Samson Henrique
SH
Lopasso
Fábio Pinatel
FP
Irya
Kiyoshi
K
D000818:Animals; D005973:Glutamine / Q000008:administration & dosage*; D022781:Hepatocytes / Q000378:metabolism*; D007421:Intestine, Small / Q000473:pathology / Q000601:surgery*; D008112:Liver Glycogen / Q000032:analysis*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015431:Weight Loss
D008112:Liver Glycogen; D005973:Glutamine