title: Regulation of cellular infiltration into tissue engineering scaffolds composed of submicron diameter fibrils produced by electrospinning.
abstract: We characterize the infiltration of interstitial cells into tissue engineering scaffolds prepared with electrospun collagen, electrospun gelatin, electrospun poly(glycolic) acid (PGA), electrospun poly(lactic) acid (PLA), and an electrospun PGA/PLA co-polymer. Electrospinning conditions were optimized to produce non-woven tissue engineering scaffolds composed of individual fibrils less than 1000 nm in diameter. Each of these materials was then electrospun into a cylindrical construct with a 2 mm inside diameter with a wall thickness of 200-250 microm. Electrospun scaffolds of collagen were rapidly, and densely, infiltrated by interstitial and endothelial cells when implanted into the interstitial space of the rat vastus lateralis muscle. Functional blood vessels were evident within 7 days. In contrast, implants composed of electrospun gelatin or the bio-resorbable synthetic polymers were not infiltrated to any great extent and induced fibrosis. Our data suggests that topographical features, unique to the electrospun collagen fibril, promote cell migration and capillary formation.
authors.affiliation: Shenandoah University Division of Physical Therapy, Winchester, VA 22601, USA. ttelemec@su.edu
authors.forename: T A
authors.initials: TA
authors.lastname: Telemeco
authors.forename: C
authors.initials: C
authors.lastname: Ayres
authors.forename: G L
authors.initials: GL
authors.lastname: Bowlin
authors.forename: G E
authors.initials: GE
authors.lastname: Wnek
authors.forename: E D
authors.initials: ED
authors.lastname: Boland
authors.forename: N
authors.initials: N
authors.lastname: Cohen
authors.forename: C M
authors.initials: CM
authors.lastname: Baumgarten
authors.forename: J
authors.initials: J
authors.lastname: Mathews
authors.forename: D G
authors.initials: DG
authors.lastname: Simpson
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012399:Rotation; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid