title: Direct analysis of a GPCR-agonist interaction by surface plasmon resonance.
abstract: Despite their clinical importance, detailed analysis of ligand binding at G-protein coupled receptors (GPCRs) has proved difficult. Here we successfully measure the binding of a GPCR, neurotensin receptor-1 (NTS-1), to its ligand, neurotensin (NT), using surface plasmon resonance (SPR). Specific responses were observed between NT and purified, detergent-solublised, recombinant NTS-1, using a novel configuration where the biotinylated NT ligand was immobilised on the biosensor surface. This SPR approach shows promise as a generic approach for the study of ligand interactions with other suitable GPCRs.
authors.affiliation: Biomembrane Structure Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Harding
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Hadingham
authors.forename: James M
authors.initials: JM
authors.lastname: McDonnell
authors.forename: Anthony
authors.initials: A
authors.lastname: Watts
mesh_terms: D000818:Animals; D007700:Kinetics; D008024:Ligands; D009496:Neurotensin / Q000737:chemistry*; D051381:Rats; D018028:Receptors, Neurotensin / Q000819:agonists* / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D008024:Ligands; D018028:Receptors, Neurotensin; C114036:neurotensin type 1 receptor; D009496:Neurotensin