Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags.
Intrahepatic cholangiocarcinoma (ICC), a malignant tumor derived from the bile duct epithelium, is one of the leading causes of death from cancer, worldwide. However, the mechanisms related to it remain largely unknown. In this study, an analysis of the gene expression profiles for ICC was done using the frequency of the ESTs obtained from nine cDNA libraries that constructed from 4 ICC cell lines and 4 normal liver tissues. One hundred and thirty-seven genes were identified as being either up- or down-regulated in human ICC cells. Thirty genes were randomly selected to confirm their differential expression in 4 human ICC cell lines and 5 ICC tissues compared to normal liver tissues by semi-quantitative RT-PCR. Among these genes, ANXA1, ANXA2, AMBP, and SERPINC1 were further verified by immunohistochemical analyses. In conclusion, these identified genes represent potential biomarkers for ICC and represent potential targets for elucidating the molecular mechanisms that are associated with ICC.
Wang
Ai-Guo
AG
Laboratory of Human Genomics, Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
Yoon
Sun Young
SY
Oh
Jung-Hwa
JH
Jeon
Yeo-Jin
YJ
Kim
Mirang
M
Kim
Jeong-Min
JM
Byun
Sang-Soon
SS
Yang
Jin Ok
JO
Kim
Joo Heon
JH
Kim
Dae-Ghon
DG
Yeom
Young-Il
YI
Yoo
Hyang-Sook
HS
Kim
Yong Sung
YS
Kim
Nam-Soon
NS
D001650:Bile Duct Neoplasms / Q000235:genetics*; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000235:genetics*; D015536:Down-Regulation; D020224:Expressed Sequence Tags; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D020022:Genetic Predisposition to Disease; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D014408:Biomarkers, Tumor