Proteomic analysis of hen egg white.
Hen egg white is an original biological fluid in which major proteins have been widely studied, unlike the minor components. In this study, two-dimensional electrophoresis associated with mass spectrometry enabled the separation of 69 protein spots and their matching with major proteins, which were already known, and with minor proteins. Sixteen proteins were identified, and among them, two had never been previously detected in hen egg white, i.e., Tenp, a protein with strong homology with a bacterial permeability-increasing protein family (BPI), and VMO-1, an outer layer vitelline membrane protein. Thirteen proteins present a very wide polymorphism (ovotransferrin, ovomucoid, clusterin, etc.), some of them up to nine isoforms (ovoinhibitor). Eleven functional protein families were identified (serpin, transferrin, protease inhibitors Kazal, glycosyl hydrolases, lipocalin, bactericidal permeability-increasing protein, clusterin, UPAR/CD59/Ly6/ snake neurotoxin, cysteine protease inhibitor, VMO-1, and folate receptor families). These various biological functions could be interesting for further valorizations. In addition, three spots remain unidentified, probably because these proteins are not yet indexed in the international protein databanks.
Guérin-Dubiard
Catherine
C
UMR 1253 INRA-Agrocampus Rennes Sciences et Technologie du Lait et de l'Oeuf, and UMR 598 INRA-Agrocampus Rennes Génétique Animale, 65 rue de Saint-Brieuc, CS 84215, 35042 Rennes Cedex, France.
Pasco
Maryvonne
M
Mollé
Daniel
D
Désert
Colette
C
Croguennec
Thomas
T
Nau
Françoise
F
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000032:analysis; D002645:Chickens; D004527:Egg Proteins / Q000032:analysis; D004529:Egg White / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis; D011506:Proteins / Q000032:analysis*
D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D004527:Egg Proteins; D008565:Membrane Proteins; D011506:Proteins; C021623:bactericidal permeability increasing protein; C035651:vitelline membrane proteins