title: Analysis of the relationship between peak stress and proteoglycan loss following injurious compression of human post-mortem knee and ankle cartilage.
abstract: While traumatic joint injuries are known to increase the risk of osteoarthritis (OA), the mechanism is not known. Models for injurious compression of cartilage may identify predictors of injury that suggest a clinical mechanism. We investigated the relationship between peak stress during compression and glycosaminoglycan (GAG) loss after injury for knee and ankle cartilages. Human cartilage explant disks were harvested post-mortem from the knee and ankle of three organ donors with no history of OA and subjected to injurious compression to 65% strain in uniaxial unconfined compression at 2 mm/s (400%/s). The GAG content of the conditioned medium was measured 3 days after injury. After injury of knee cartilage disks, damage was visible in 18 of 39 disks (36%). Three days after injury, the increase in GAG loss to the medium (GAG loss from injured disks minus GAG loss from location-matched uncompressed controls) was 1.5+/-0.3 microg/disk (mean +/- SEM). With final strain and compression velocity held constant, we observed that increasing peak stress during injury was associated with less GAG loss after injury (P<0.001). In contrast, ankle cartilage appeared damaged after injury in only 1 of 16 disks (6%), there was no increase in GAG loss (0.0+/-0.3 microg/disk), and no relationship between peak stress and increase in GAG loss was detected (P=0.51). By itself, increasing peak stress did not appear to be an important cause of GAG loss from human cartilage in our injurious compression model. However, we observed further evidence for differences in the response of knee and ankle cartilages to injury.
authors.affiliation: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA. ppatwari@rics.bwh.harvard.edu
authors.forename: Parth
authors.initials: P
authors.lastname: Patwari
authors.forename: Debbie M
authors.initials: DM
authors.lastname: Cheng
authors.forename: Ada A
authors.initials: AA
authors.lastname: Cole
authors.forename: Klaus E
authors.initials: KE
authors.lastname: Kuettner
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Grodzinsky
mesh_terms: D000842:Ankle / Q000473:pathology*; D002356:Cartilage / Q000473:pathology*; D019245:Compressive Strength; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D007717:Knee / Q000473:pathology*; D011509:Proteoglycans / Q000378:metabolism*; D012044:Regression Analysis; D013314:Stress, Mechanical
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans