title: Antihypertensive effect of angiotensin I-converting enzyme inhibitory peptides from a sesame protein hydrolysate in spontaneously hypertensive rats.
abstract: Sesame peptide powder (SPP) exhibited angiotensin I-converting enzyme (ACE) inhibitory activity, and significantly and temporarily decreased the systolic blood pressure (SBP) in spontaneously hypertensive rats (SHRs) by a single administration (1 and 10 mg/kg). Six peptide ACE inhibitors were isolated and identified from SPP. The representative peptides, Leu-Val-Tyr, Leu-Gln-Pro and Leu-Lys-Tyr, could competitively inhibit ACE activity at respective Ki values of 0.92 microM, 0.50 microM, and 0.48 microM. A reconstituted sesame peptide mixture of Leu-Ser-Ala, Leu-Gln-Pro, Leu-Lys-Tyr, Ile-Val-Tyr, Val-Ile-Tyr, Leu-Val-Tyr, and Met-Leu-Pro-Ala-Tyr according to their content ratio in SPP showed a strong antihypertensive effect on SHR at doses of 3.63 and 36.3 microg/kg, which accounted for more than 70% of the corresponding dosage for the SPP-induced hypotensive effect. Repeated oral administration of SPP also lowered both SBP and the aortic ACE activity in SHR. These results demonstrate that SPP would be a beneficial ingredient for preventing and providing therapy against hypertension and its related diseases.
authors.affiliation: Department of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nakano
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Ogura
authors.forename: Masazumi
authors.initials: M
authors.lastname: Miyakoshi
authors.forename: Fumie
authors.initials: F
authors.lastname: Ishii
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kawanishi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kurumazuka
authors.forename: Chol-Jun
authors.initials: CJ
authors.lastname: Kwak
authors.forename: Kenji
authors.initials: K
authors.lastname: Ikemura
authors.forename: Masanori
authors.initials: M
authors.lastname: Takaoka
authors.forename: Shigeo
authors.initials: S
authors.lastname: Moriguchi
authors.forename: Taeko
authors.initials: T
authors.lastname: Iino
authors.forename: Aki
authors.initials: A
authors.lastname: Kusumoto
authors.forename: Sumio
authors.initials: S
authors.lastname: Asami
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shibata
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kiso
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Matsumura
mesh_terms: D000284:Administration, Oral; D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D006868:Hydrolysis; D006973:Hypertension / Q000201:enzymology*; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007703:Peptidyl-Dipeptidase A / Q000187:drug effects*; D028321:Plant Preparations / Q000008:administration & dosage / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D011918:Rats, Inbred SHR; D031685:Sesamum / Q000737:chemistry*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D009842:Oligopeptides; D028321:Plant Preparations; D010940:Plant Proteins; D007703:Peptidyl-Dipeptidase A