Quantification of ganciclovir in human plasma using capillary electrophoresis.
A fast, simple, specific capillary electrophoretic method in the MEKC mode for the quantification of the antiviral drug ganciclovir is described. The separation was obtained using a 50 microm id fused-silica capillary, 60 mM borax buffer (pH 9.25) containing 40 mM SDS using ethenoadenosine as the internal standard. Sample preparation was done by ultrafiltration with a Microcon 30 000 kDa filter. The analytes were detected with UV detector at 254 nm. A sufficient sensitivity was achieved by using a bubble cell capillary. The linear range was from 0.5 to 10 mg/L with a LOQ of 0.5 mg/L. Correlation coefficients were better than 0.999 whereas inter- and intraday precision and accuracy were less than 10.7%. The analysis of patients' samples after administration of ganciclovir indicates that the method is suitable for drug monitoring in the clinic.
Saleh
Soundos
S
Klinik und Poliklinik für Kinder- und Jugendmedizin--Pädiatrische Hämatologie und Onkologie, Münster, Germany.
Hempel
Georg
G
D000998:Antiviral Agents / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods*; D015774:Ganciclovir / Q000097:blood*; D006801:Humans; D010472:Perchlorates / Q000737:chemistry; D012680:Sensitivity and Specificity; D014238:Trichloroacetic Acid / Q000737:chemistry; D014462:Ultrafiltration
D000998:Antiviral Agents; D010472:Perchlorates; D014238:Trichloroacetic Acid; D015774:Ganciclovir