title: Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin.
abstract: We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (Kd) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.
authors.affiliation: Institute for Antibodies Ltd., Toyoake, Aichi 470-1192, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Kakita
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Takako
authors.initials: T
authors.lastname: Komiya
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Iba
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuji
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kurosawa
authors.forename: Motohide
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D004167:Diphtheria Toxin / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011817:Rabbits
chemical_list: D000911:Antibodies, Monoclonal; D004167:Diphtheria Toxin