Altered expression of goblet cell- and mucin glycosylation-related genes in the intestinal epithelium during infection with the nematode Nippostrongylus brasiliensis in rat.
Intestinal nematode infection induces marked goblet cell hyperplasia and mucus secretion, but the mechanisms of regulation of the changes still remain to be elucidated. In the present study, epithelial cells were isolated from the rat small intestine at various times after Nippostrongylus brasiliensis infection, and the levels of expression of goblet cell- and mucin glycosylation-related genes were estimated by semi-quantitative reverse transcription (RT)-PCR. Among the genes investigated, mucin core peptide (MUC) 2, sialyltransferase (Siat) 4c and trefoil factor family (TFF) 3 were upregulated as early as 2-4 days post-infection, suggesting that they are associated with an early innate protective response. Seven days post-infection and thereafter, when the nematodes reached maturity, significant upregulation of MUC3, MUC4, resistin-like molecule beta (Relmbeta) and 3O-sulfotransferase (3ST)1 was observed, while 3ST2 expression levels increased after the majority of the worms were expelled from the intestine. Similar alterations of glycosylation-related gene expression were also observed in mast-cell-deficient Ws/Ws rats, suggesting that mast cells in the epithelium are not relevant to the upregulation of these genes. The present finding that the expression level of each goblet cell- or glycosylation-related gene was altered differently during the time course of infection indicates the progression of sequential qualitative changes in the mucus layer after infection.
Yamauchi
Junko
J
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Kawai
Yuichi
Y
Yamada
Minoru
M
Uchikawa
Ryuichi
R
Tegoshi
Tatsuya
T
Arizono
Naoki
N
D000818:Animals; D005786:Gene Expression Regulation; D006031:Glycosylation; D020397:Goblet Cells / Q000378:metabolism / Q000469:parasitology*; D006729:Hormones, Ectopic / Q000235:genetics; D007411:Intestinal Diseases, Parasitic / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000469:parasitology*; D007421:Intestine, Small / Q000378:metabolism / Q000469:parasitology; D055262:Mucin-2; D055264:Mucin-4; D009077:Mucins / Q000235:genetics / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics; D009559:Nippostrongylus; D051381:Rats; D012376:Rodent Diseases / Q000235:genetics* / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics; D017206:Strongylida Infections / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D015238:Sulfotransferases / Q000235:genetics; D000071165:Trefoil Factor-3; D015854:Up-Regulation; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
D006729:Hormones, Ectopic; C524628:Muc2 protein, rat; C524660:Muc4 protein, rat; D055262:Mucin-2; D055264:Mucin-4; D009077:Mucins; D009479:Neuropeptides; C099037:TFF3 protein, rat; D000071165:Trefoil Factor-3; D012799:Sialyltransferases; D015238:Sulfotransferases; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase