title: Brain alpha-dystroglycan displays unique glycoepitopes and preferential binding to laminin-10/11.
abstract: alpha-Dystroglycan was quantitatively enriched from mammalian brain based on its uniform reactivity with Vicia villosa agglutinin and resolved into sub-populations possessing or lacking the sulfated glucuronic acid epitope recognized by monoclonal antibody HNK-1. We generated a new monoclonal antibody specific for a glycoepitope on brain alpha-dystroglycan but absent from alpha-dystroglycan expressed in all other tissues examined. Finally, we found that laminin-10/11 preferentially bound to brain alpha-dystroglycan compared to skeletal muscle alpha-dystroglycan. Our results suggest that tissue-specific glycosylation modifies the laminin binding specificity of alpha-dystroglycan.
authors.affiliation: Department of Physiology, University of Wisconsin, Madison, 53706, USA.
authors.forename: Erin L
authors.initials: EL
authors.lastname: McDearmon
authors.forename: Ariana C
authors.initials: AC
authors.lastname: Combs
authors.forename: Kiyotoshi
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: Hironobu
authors.initials: H
authors.lastname: Fujiwara
authors.forename: James M
authors.initials: JM
authors.lastname: Ervasti
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D049030:Dystroglycans / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism; D011817:Rabbits
chemical_list: D049030:Dystroglycans