title: Roles of sumoylation of a reptin chromatin-remodelling complex in cancer metastasis.
abstract: Defining the functional modules within transcriptional regulatory factors that govern switching between repression and activation events is a central issue in biology. Recently, we have reported the dynamic role of a beta-catenin-reptin chromatin remodelling complex in regulating a metastasis suppressor gene KAI1 (ref.1), which is capable of inhibiting the progression of tumour metastasis. Here, we identify signalling factors that confer repressive function on reptin and hence repress the expression of KAI1. Biochemical purification of a reptin-containing complex has revealed the presence of specific desumoylating enzymes that reverse the sumoylation of reptin that underlies its function as a repressor. Desumoylation of reptin alters the repressive function of reptin and its association with HDAC1. Furthermore, the sumoylation status of reptin modulates the invasive activity of cancer cells with metastatic potential. These data clearly define a functional model and provide a novel link for SUMO modification in cancer metastasis.
authors.affiliation: Department of Biological Sciences, Research Center for Functional Cellulomics, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Kim
authors.forename: Hee June
authors.initials: HJ
authors.lastname: Choi
authors.forename: Bogyou
authors.initials: B
authors.lastname: Kim
authors.forename: Mi Hyang
authors.initials: MH
authors.lastname: Kim
authors.forename: Ji Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Ik Soo
authors.initials: IS
authors.lastname: Kim
authors.forename: Moon Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Soo Joon
authors.initials: SJ
authors.lastname: Choi
authors.forename: Keun Il
authors.initials: KI
authors.lastname: Kim
authors.forename: Su-Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Chin Ha
authors.initials: CH
authors.lastname: Chung
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Baek
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D002352:Carrier Proteins / Q000378:metabolism / Q000502:physiology*; D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism*; D004265:DNA Helicases / Q000378:metabolism / Q000502:physiology*; D005786:Gene Expression Regulation; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000378:metabolism; D006801:Humans; D051861:Kangai-1 Protein / Q000235:genetics; D009362:Neoplasm Metastasis; D011485:Protein Binding; D012097:Repressor Proteins; D015398:Signal Transduction; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*
chemical_list: C497688:CD82 protein, human; D002352:Carrier Proteins; D002843:Chromatin; D051861:Kangai-1 Protein; D012097:Repressor Proteins; D025841:Small Ubiquitin-Related Modifier Proteins; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases; D000074183:ATPases Associated with Diverse Cellular Activities; D004265:DNA Helicases; C122079:RUVBL2 protein, human