title: Diagnosis of deep-seated lymphoma and leukemia by endoscopic ultrasound-guided fine-needle aspiration biopsy.
abstract: We retrospectively studied the use of endoscopic ultrasound-guided fine-needle aspiration biopsy (EUS-FNAB) as a tool for the diagnosis of deep-seated lymphoma. An on-site assessment at the time of EUS-FNAB was performed by a cytopathologist using Diff-Quik (American Scientific Products, McGraw Park, IL) stain. In addition, Papanicolaou stains were performed on EUS-FNAB smears, immunohistochemical stains were performed on cell blocks, and additional samples were sent for flow cytometric analysis. Final cytologic diagnosis was correlated with surgical pathology and/or clinical follow-up. We evaluated EUS-FNAB specimens of deep-seated lymph nodes, spleen, stomach, and pancreas, and 1 EUS-guided needle core biopsy specimen of a lymph node. Thirteen cases of deep-seated lymphoma were diagnosed, including non-Hodgkin lymphomas and Hodgkin lymphoma. One case of hairy cell leukemia was diagnosed. EUS-FNAB is a minimally invasive, cost-effective, and useful tool for the primary diagnosis or staging of deep-seated lymphomas.
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, 619 19th Street South, Birmingham, AL 35233, USA.
authors.forename: Judith L
authors.initials: JL
authors.lastname: Pugh
authors.forename: Nirag C
authors.initials: NC
authors.lastname: Jhala
authors.forename: Mohamad A
authors.initials: MA
authors.lastname: Eloubeidi
authors.forename: David C
authors.initials: DC
authors.lastname: Chhieng
authors.forename: Isam A
authors.initials: IA
authors.lastname: Eltoum
authors.forename: D Ralph
authors.initials: DR
authors.lastname: Crowe
authors.forename: Shyam
authors.initials: S
authors.lastname: Varadarajulu
authors.forename: Darshana N
authors.initials: DN
authors.lastname: Jhala
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044963:Biopsy, Fine-Needle; D019160:Endosonography / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007943:Leukemia, Hairy Cell / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D012680:Sensitivity and Specificity