A novel sporadic Burkitt lymphoma cell line (BLUE-1) with a unique t(6;20)(q15;q11.2) rearrangement.
We report the establishment and characterization, including HLA-typing, immunophenotypic and molecular cytogenetic analysis, of a novel EBV-negative cell line (BLUE-1) derived from adult relapsed sporadic Burkitt lymphoma. BLUE-1 carries the pathognomonic t(8;14)(q24;q32) effecting MYC/IgHJ fusion and a novel t(6;20)(q15;q11.2) originally present in the patient, analysis of which may facilitate identification of gene target(s) of recurrent 6q rearrangements in B-cell neoplasia. Our findings are discussed in light of the current understanding of endemic and sporadic Burkitt lymphoma. BLUE-1 grows well in culture and should be a useful lymphoma research tool.
Burmeister
Thomas
T
Medizinische Klinik III, Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany. thomas.burmeister@charite.de <thomas.burmeister@charite.de>
Macleod
Roderick A F
RA
Reinhardt
Richard
R
Mansmann
Veit
V
Loddenkemper
Christoph
C
Marinets
Olga
O
Drexler
Hans G
HG
Thiel
Eckhard
E
Blau
Igor Wolfgang
IW
D000328:Adult; D002051:Burkitt Lymphoma / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D045744:Cell Line, Tumor; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D020732:Cytogenetic Analysis / Q000379:methods; D006680:HLA Antigens / Q000235:genetics; D006650:Histocompatibility Testing; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D014178:Translocation, Genetic / Q000235:genetics*
D006680:HLA Antigens