[The role of JWA in N-methyl-N'-nitro-N-nitrosoguanidine induced human bronchial epithelial cell apoptosis].
OBJECTIVE
To investigate the role and possible mechanism of JWA in N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced human bronchial epithelial (HBE) cell apoptosis.


METHODS
The cell growth inhibition rate was detected by MTT, the cell apoptosis was measured by Hoechst staining, the expression of JWA protein was detected by Western blot, and the potential binding protein of JWA proximal promoter was detected by Southwestern assay.


RESULTS
MNNG treatment of HBE cells for 24 hours induced apoptosis with significant dose-effect relationship and in this course the expression of JWA protein was elevated. The 2.0 microg/ml MNNG treated cells for 24 hours activated nuclear transcription factor expression that specifically bound to JWA proximal promoter.


CONCLUSIONS
That MNNG treatment activates nuclear transcription factor binding to JWA proximal promoter may be involved in intracellular apoptosis associated signal pathway.
Xu
Yan-qiong
YQ
Department of Molecular Cell Biology & Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
Li
Ai-ping
AP
Chen
Rui
R
Zhou
Jian-wei
JW
D017209:Apoptosis; D001980:Bronchi / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000502:physiology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000502:physiology*; D026901:Membrane Transport Proteins; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism
C484566:ARL6IP5 protein, human; D006360:Heat-Shock Proteins; D047908:Intracellular Signaling Peptides and Proteins; D026901:Membrane Transport Proteins; D014157:Transcription Factors; D008769:Methylnitronitrosoguanidine