title: Xenopus ADAMTS1 negatively modulates FGF signaling independent of its metalloprotease activity.
abstract: We have isolated the Xenopus ortholog of ADAMTS1 (a disintegrin and metalloprotease with thrombospondin motifs), XADAMTS1, which is expressed in the presumptive ectoderm, then the Spemann organizer, and later in the trunk organizer region and posterior ectoderm in the Xenopus embryo. We show that, when overexpressed in the dorsal marginal zone or in the anterior ectoderm by mRNA injection, XADAMTS1 inhibits gastrulation or generates embryos with an enlarged cement gland, respectively. XADAMTS1 also reduces the expression of Xbra in both whole embryos and FGF-treated animal caps. These effects of XADAMTS1 are likely to be due to its inhibition of the Ras-MAPK cascade because XADAMTS1 inhibits the phosphorylation of ERK by FGF4 in animal caps. Deletion analysis of XADAMTS1 revealed that a combination of the signal peptide and the C-terminal region containing the thrombospondin type 1 repeats is necessary and sufficient for this function, whereas the metalloprotease domain is dispensable. In addition, loss-of-function analysis with antisense morpholino oligos showed that knockdown of XADAMTS1 sensitizes animal caps to Xbra induction by FGF2. These data suggest that secreted XADAMTS1 negatively modulates FGF signaling in the Xenopus embryo.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Suga
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hikasa
authors.forename: Masanori
authors.initials: M
authors.lastname: Taira
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D000071097:ADAMTS1 Protein; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004475:Ectoderm / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D005775:Gastrula / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006493:Heparin / Q000378:metabolism; D008648:Mesoderm / Q000378:metabolism; D045726:Metalloproteases / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D015398:Signal Transduction; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D014981:Xenopus / Q000196:embryology / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020825:T-Box Domain Proteins; C116765:TBXT protein, Xenopus; D029867:Xenopus Proteins; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors; D006493:Heparin; D045726:Metalloproteases; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C512854:ADAMTS1 protein, Xenopus