Octyl-functionalized hybrid silica monolithic column for reversed-phase capillary electrochromatography.
Hybrid silica monolithic stationary phase functionalized with octyl groups was synthesized by a two-step acid/base-catalyzed hydrolysis/co-condensation of tetraethoxysilane (TEOS) and n-octyltriethoxysilane (C(8)-TEOS). The influences of determining factors in the sol-gel process such as the monomer ratio and water content on the monolith formation were systematically investigated. An increase in the TEOS/C(8)-TEOS ratio in the polymerization mixture shifted the pore size distribution towards smaller pore diameter with larger pore volume. The optimal TEOS/C(8)-TEOS volume ratio was found to be 90/50, under which condition the median pore diameter of the monolith was around 1.0 microm with pore volume of 3.25 cm(3)/g. The chromatographic characteristics of the monolithic column prepared with the optimized fabrication condition were studied. Some aromatic compounds including alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs) and phenols were successfully separated on the octyl-functionalized silica monolithic column with high column efficiency up to 180,000 plates/m.
Yan
Li-Juan
LJ
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Zhang
Qing-He
QH
Feng
Yu-Qi
YQ
Zhang
Wei-Bing
WB
Li
Tong
T
Zhang
Li-Hua
LH
Zhang
Yu-Kui
YK
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D012822:Silicon Dioxide / Q000737:chemistry*
D012822:Silicon Dioxide