A bioconjugate of Lys and Arg mimics biological activity of the Arg-Gly-Asp peptide sequence.
A peptidomimetic, 2-amino-6-[(2-amino-5{guanidino}pentanoyl) amino] hexanoic acid, was synthesized using Lys and Arg to produce a compound that mimics the biological activity of a cell adhesive Arg-Gly-Asp (RGD) peptide, GRGDSP. When immobilized on solid substrates, the peptidomimetic promoted cell adhesion similar to substrates with immobilized GRGDSP. Ligand competition studies demonstrated that cell interactions with the peptidomimetic were integrin-mediated. The peptidomimetic was very stable to proteolytic degradation in comparison to proteolytically unstable peptides. Both GRGDSP and peptidomimetic were stabilized when immobilized on solid substrates. This peptidomimetic has the broad therapeutic utility of the RGD peptides with higher stability and potentially enhanced therapeutic efficacy.
Ehteshami
Gholamreza
G
Harrington Department of Bioengineering, Arizona State University, P.O. Box 879709, Tempe, AZ 85287-9709, USA.
Brune
Daniel C
DC
Lopez
John C
JC
Massia
Stephen P
SP
D016475:3T3 Cells; D000818:Animals; D001120:Arginine / Q000737:chemistry; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D004355:Drug Stability; D008239:Lysine / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D018716:Molecular Mimicry; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D012996:Solutions
D001672:Biocompatible Materials; D009842:Oligopeptides; D012996:Solutions; C047981:arginyl-glycyl-aspartic acid; D001120:Arginine; D008239:Lysine