Drosophila homologue of Eps15 is essential for synaptic vesicle recycling.
The mammalian protein Eps15 is phosphorylated by EGF receptor tyrosine kinase and has been shown to interact with several components of the endocytic machinery. We have identified a hypomorphic Eps15 mutant in Drosophila which shows reversible paralysis and an altered physiology at restrictive temperatures. In addition, the temperature-sensitive paralytic defect of shibire mutant is enhanced by this mutant. Eps15 is enriched in the larval neuromuscular junction in endocytic 'hot spots' in a pattern similar to Dynamin. Eps15 mutants show a decrease in the alpha-Adaptin levels at the larval neuromuscular junction synapse. Genetic and biochemical studies of interactions with components of the endocytic machinery suggest that Eps15 has an important role in synaptic vesicle recycling and regulates recruitment of alpha-Adaptin.
Majumdar
Amitabha
A
Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba Mumbai 400005, India. amitavamajumdar@gmail.com
Ramagiri
Shobha
S
Rikhy
Richa
R
D033965:Adaptor Protein Complex alpha Subunits / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D004330:Drosophila / Q000235:genetics / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034281:Dynamins / Q000235:genetics / Q000378:metabolism; D004596:Electroretinography; D004705:Endocytosis / Q000235:genetics / Q000502:physiology; D008854:Microscopy, Electron; D009154:Mutation / Q000235:genetics; D052066:N-Ethylmaleimide-Sensitive Proteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D011726:Pyridinium Compounds / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013572:Synaptic Vesicles / Q000502:physiology*; D013696:Temperature
D033965:Adaptor Protein Complex alpha Subunits; D002352:Carrier Proteins; D029721:Drosophila Proteins; C512908:Eps-15 protein, Drosophila; C073804:FM1 43; D009419:Nerve Tissue Proteins; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; C498732:comt protein, Drosophila; C420114:stnB protein, Drosophila; D052066:N-Ethylmaleimide-Sensitive Proteins; D034281:Dynamins; C439628:shi protein, Drosophila