Loss of promyelocytic leukemia protein in human gastric cancers.
To clarify the clinical implications of promyelocytic leukemia (PML) expression in gastric carcinomas, the expression of PML was analyzed in large series of gastric carcinoma by immunohistochemistry, western blotting and reverse transcription-PCR. PML protein expression was reduced or abolished in gastric carcinomas (31.7 and 10.6%, respectively) by immunohistochemistry. PML protein loss was associated with more lymphatic invasion, higher pTNM stage, and worse patient survival. Only one gastric carcinoma cell line showed loss of PML, and the PML protein re-appeared after the treatment of proteasome inhibitor in this cell line. We conclude that PML protein loss occurs in a minority of gastric carcinomas during carcinogenesis and progression, and suggest the proteasome-dependent pathway as a mechanism of PML protein loss.
Lee
Hee Eun
HE
Department of Pathology, Seoul National University College of Medicine, Seoul, South Korea.
Jee
Chang Do
CD
Kim
Min A
MA
Lee
Hye Seung
HS
Lee
You Mie
YM
Lee
Byung Lan
BL
Kim
Woo Ho
WH
D002277:Carcinoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000172:deficiency / Q000378:metabolism*; D009687:Nuclear Proteins / Q000172:deficiency / Q000378:metabolism*; D000071156:Promyelocytic Leukemia Protein; D013274:Stomach Neoplasms / Q000378:metabolism*; D014157:Transcription Factors / Q000172:deficiency / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000172:deficiency / Q000378:metabolism*
D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human