Quantitative expression analysis of inulinase gene cluster of Penicillium sp. strain TN-88.
The filamentous fungus Penicillium sp. strain TN-88 carries the endoinulinase gene inuC and the exoinulinase gene inuD that are linked head-to-head on the genome and divergently transcribed from an 859-bp intergenic region [Moriyama et al., Biosci. Biotechnol. Biochem., 66, 1887-1896 (2002)]. Quantitative real-time PCR amplification revealed that the transcription levels of the inuC and inuD genes increased 42- and 3260-fold in inulin-grown mycelia, respectively. Sucrose as well as fructose did not induce the expression of the inuC or inuD gene at all. The levels of inuC and inuD transcripts in mycelia grown on the glucose/inulin mixture were both below their basal levels in glucose-grown mycelia. Thus, glucose exerts a strong carbon catabolite repression on the expression of the two genes.
Moriyama
Satoshi
S
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.
Muguruma
Michio
M
Ohta
Kazuyoshi
K
D002244:Carbon / Q000378:metabolism; D003062:Codon; D005632:Fructose / Q000737:chemistry; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics*; D008957:Models, Genetic; D005810:Multigene Family; D010407:Penicillium / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013395:Sucrose / Q000737:chemistry; D014158:Transcription, Genetic
D003062:Codon; D005656:Fungal Proteins; D005632:Fructose; D013395:Sucrose; D002244:Carbon; D006026:Glycoside Hydrolases; C421180:InuC protein, Penicillium; C023086:inulinase; D005947:Glucose