[Effect of mica granule on the expression of gene-protein associated with cancer in gastric mucosa tissue of chronic atrophic gastritis rats].
OBJECTIVE
To research the regulative effect of mica monomer granule preparation on the expression of gene associated with cancer in gastric mucosa tissue of experimental chronic atrophic gastritis (CAG) rats.


METHODS
To treat experimental CAG rats using mica monomer granule preparation with three different dosage-high, moderate and low level respectively. To observe the expression changes of mutant antioncogene-p53 gene-protein, oncogene p21, antioncogene p16 and anti-apoptosis gene bcl-2 in gastric mucosa of CAG rats by two-step ways of EnVision system in immunohistochemical method.


RESULTS
There was the tendency that mica monomer granule preparation with three different dosage could decrease the expression of p53 as well as p21, and mica had the obvious regulative effects on deletion of p16 and high-expression of bcl-2. It could also alleviate the inflammation of gastric mucosa and promote the regeneration of gland.


CONCLUSIONS
The treatment and reversion action of mica on chronic atrophic gastritis is probably related with the regulative effect on the expression of gene associated with cancer.
Zhu
Fang-Shi
FS
Academy of Traditional Chinese Medicine of Jiangsu Province, Nanjing 210028, China. zhufs@tom.net
Si
Jian-Min
JM
Wang
Liang-Jing
LJ
Wang
Dong-Fei
DF
Chen
Pin
P
D000538:Aluminum Silicates / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D005757:Gastritis, Atrophic / Q000378:metabolism* / Q000473:pathology; D008419:Materia Medica / Q000008:administration & dosage / Q000494:pharmacology*; D015689:Oncogene Protein p21(ras) / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
D000538:Aluminum Silicates; D019941:Cyclin-Dependent Kinase Inhibitor p16; D008419:Materia Medica; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D015689:Oncogene Protein p21(ras); C011934:mica