Study of cellulolytic soil fungi and two nova species and new medium.
This study is aimed at identifying and determining the percentage of occurrence frequency of cellulose decomposing soil fungi. The soil samples were inoculated into culture plates prepared in Sabouraud medium under sterilized conditions and incubated at 30 degrees C for 4 to 7 d. The identified fungal species were incubated in self-designed cellulose medium for testing their cellulolytic ability. Forty-two species, including 2 nova species, representing sixteen genera showed growth and sporulation in the cellulose medium. Most of the isolated species were from genus Aspergillus and Penicillium. Aspergillus niger and Mucor hiemalis showed highest occurrence frequency (45% and 36% respectively), as these species were collected from about 80% of soil samples. Being agar free and cheaper, the new fungal medium designed showed results equivalent to Sabouraud medium.
Khalid
Mahmood
M
Government College, Satellite Town, Rawalpindi 46000, Pakistan. kmahdi2005@yahoo.com
Yang
Wei-Jun
WJ
Kishwar
Nazir
N
Rajput
Zahid Iqbal
ZI
Arijo
Abdullah G
AG
D001230:Aspergillus / Q000302:isolation & purification / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media; D003904:Mitosporic Fungi / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D010407:Penicillium / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology
D003470:Culture Media; D002482:Cellulose