Peroxisome is a reservoir of intracellular calcium.
We have examined fura 2-loaded purified peroxisomes under confocal microscope to prove that this mammalian organelle is a store of intracellular calcium pool. Presence of calcium channel and vanadate sensitive Ca(2+)-ATPase in the purified peroxisomal membrane has been demonstrated. We have further observed that machineries to maintain calcium pool in this mammalian organelle are impaired during infection caused by Leishmania donovani. Results reveal that peroxisomes have a merit to play a significant role in the metabolism of intracellular calcium.
Raychaudhury
Bikramjit
B
Department of Biological Chemistry, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Kolkata 700032, India.
Gupta
Shreedhara
S
Banerjee
Shouvik
S
Datta
Salil C
SC
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism / Q000469:parasitology; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D007893:Leishmania donovani / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D018613:Microscopy, Confocal; D020675:Peroxisomes / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D014638:Vanadates / Q000494:pharmacology
D015220:Calcium Channels; D014638:Vanadates; D002118:Calcium