Tissue-specific effect of age and caloric restriction diet on mitochondrial DNA content.
The effect of age and caloric-restriction (CR) diet on mitochondrial DNA (mtDNA) content in different rat tissues was investigated. A decrease of the mtDNA content occurs with aging in liver and soleus muscle, whereas there is no age-related significant change of mtDNA content in brain. CR fully reverses the age-dependent loss of mtDNA in liver and soleus, whereas it results in a significant increase of mtDNA amount above the value of aged ad libitum fed rats in brain. These results further support the tissue-specific effect of CR, likely because of the different dependence of tissues on external nutrient uptake.
Cassano
P
P
Department of Biochemistry and Molecular Biology, University of Bari, Bari, Italy. p.cassano@biologia.uniba.it
Sciancalepore
A G
AG
Lezza
A M S
AM
Leeuwenburgh
C
C
Cantatore
P
P
Gadaleta
M N
MN
D000367:Age Factors; D000375:Aging / Q000502:physiology; D000818:Animals; D031204:Caloric Restriction; D004261:DNA Replication / Q000502:physiology; D004272:DNA, Mitochondrial / Q000032:analysis*; D018482:Muscle, Skeletal / Q000378:metabolism; D051381:Rats
D004272:DNA, Mitochondrial