[A cytological, immunophenotypical and cytogenetical study of 136 consecutive cases of B-cell chronic lymphoid hemopathies].
A cytological, immunophenotypical and cytogenetical study of 136 chronic B-cell proliferations (93 CLL, 43 B-cell lymphomas) was led in order to precise diagnosis and to characterize and appreciate chromosomal rearrangements. In this series, mainly selected on blood lymphocytosis criteria, B-CLL were twice more frequent than small B-cell lymphomas. Probes used revealed cryptic abnormalities, which remained unknown by conventional cytogenetics (CC). The frequency of clonal abnormalities (CC and FISH) was 74.8% for this series, with 74.4% for lymphomas and 75.3% for CLL, mainly of Binet stage A (69 A, 13 B, 1 C, 10 unspecified). Proportion was 88.4% in A stages and 84.6% in B stages. In CLL, 13q14 cryptic deletions and translocations were widely majority, 14q32 translocations and trisomy 12 being predominant in lymphoma series. Interphase FISH study of non-clonal metaphasic abnormalities with locus-specific probes often revealed unrecognised clones.
Struski
S
S
Laboratoire d'Hématologie, Service d'Hématologie Biologique, Pôle d'Oncohématologie, Hôpital de Hautepierre, avenue Molière, 67092 Strasbourg cedex, France.
Leymarie
V
V
Helias
C
C
Falkenrodt
A
A
Fohrer
C
C
Audhuy
B
B
Lioure
B
B
Moskovtchenko
P
P
Mazurier
I
I
Galoisy
A-C
AC
Gervais
C
C
Mauvieux
L
L
Herbrecht
R
R
Bergerat
J-P
JP
Lessard
M
M
D000782:Aneuploidy; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000235:genetics* / Q000648:ultrastructure; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics / Q000648:ultrastructure; D002999:Clone Cells / Q000473:pathology; D015331:Cohort Studies; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000235:genetics; D008297:Male; D009367:Neoplasm Staging; D017384:Sequence Deletion