title: Purification of the skeletal muscle protein Endopin 1B and characterization of the genes encoding Endopin 1A and 1B isoforms.
abstract: In the present work, a new endopin-like serpin designed mEndopin 1B was purified from bovine muscle. Biochemical characterizations (amino acid sequencing and Maldi-Tof mass spectrometry peptide mapping) demonstrated that the purified protein is different from the previously described Endopin 1, renamed mEndopin 1A. The genes and cDNA of both endopins were characterized. The cDNA sequence of mEndopin 1B encodes a predicted protein of 411 amino-acids with a molecular mass of 43808Da. The mEndopin 1B gene comprised four coding exons and an additional 5' untranslated exon. The reactive site sequence of mEndopin 1B is somewhat different from that of mEndopin 1A. Nevertheless, both serpins have a similar peptidase inhibitory pattern against examined proteases (elastase, trypsin, plasmin and chymotrypsin). The high expression of both mEndopin 1A and 1B in bovine serum and tissues and their high efficiency to inhibit elastase (k(ass) approximately 10(6)-10(7) M(-1) s(-1)) suggested that these serpins might play a major role in inflammatory processes.
authors.affiliation: QuaPA, BPM, INRA de Clermont Ferrand - Theix, Saint Genès Champanelle, France.
authors.forename: Carlos H
authors.initials: CH
authors.lastname: Herrera-Mendez
authors.forename: Laure
authors.initials: L
authors.lastname: Brémaud
authors.forename: Gerald
authors.initials: G
authors.lastname: Coulis
authors.forename: Patrick
authors.initials: P
authors.lastname: Pélissier
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Sentandreu
authors.forename: Laurent
authors.initials: L
authors.lastname: Aubry
authors.forename: Didier
authors.initials: D
authors.lastname: Delourme
authors.forename: Christophe
authors.initials: C
authors.lastname: Chambon
authors.forename: Abderrahman
authors.initials: A
authors.lastname: Maftah
authors.forename: Hubert
authors.initials: H
authors.lastname: Leveziel
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ouali
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D015843:Serpins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D014357:Trypsin / Q000187:drug effects
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D020033:Protein Isoforms; C402207:SERPINA3 protein, Bos taurus; D015843:Serpins; D010196:Pancreatic Elastase; D014357:Trypsin