title: Peptide vector for gene delivery with high affinity for phosphatidylserine.
abstract: Since phosphatidylserine (PS) is known to translocate to the external face of the plasma membrane when the cell membrane becomes disordered, we decided to focus our attention on PS as a target molecule for gene delivery. In this paper, the novel peptide Td3701 was designed, synthesized, and characterized for its physico-chemico-biological properties. Td3701 simultaneously exhibited both characters as a DNA carrier and a sensor probe for active targeting, which seemed to be triggered by structural changes in the presence of PS. This is a very unique character among nonviral vectors, and it is believed that Td3701 could be used for selective gene delivery.
authors.affiliation: Pharmaceutical Research Center, Mochida Pharmaceutical Co., Ltd., Gotemba 412-8524, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kuriyama
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Taguchi
authors.forename: Kanako
authors.initials: K
authors.lastname: Nishimura
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Mizuguchi
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Katayama
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Yanagibashi
authors.forename: Takuro
authors.initials: T
authors.lastname: Niidome
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002522:Chlorocebus aethiops; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005169:Factor VIII / Q000737:chemistry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000378:metabolism*; D008081:Liposomes; D008969:Molecular Sequence Data; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D014162:Transfection; D014709:Vero Cells
chemical_list: D008081:Liposomes; D010455:Peptides; D010718:Phosphatidylserines; D005169:Factor VIII; D004247:DNA