Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: use for comparison of antigen efficacy.
Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5 degrees C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development.
Scheel
Christina M
CM
Department of Cellular Biology, University of Georgia, Athens, USA. zsr3@cdc.gov
Handali
Sukwan
S
Bueno
Edneia C
EC
Khan
Azra
A
Hancock
Kathy
K
Gonzalez
Armando E
AE
Garcia
Hector H
HH
Gilman
Robert H
RH
Tsang
Victor C W
VC
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D012015:Reference Standards; D041201:Taenia solium / Q000276:immunology; D013997:Time Factors
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G