CHOP is involved in neuronal apoptosis induced by neurotrophic factor deprivation.
Neurotrophic factors are essential for the survival of neurons. We found that the endoplasmic reticulum (ER) stress-C/EBP homologues protein (CHOP) pathway to be activated during neurotrophic factor deprivation-induced apoptosis in PC12 neuronal cells and in primary cultured neurons, and this apoptosis was suppressed in the neurons from chop(-/-) mice. In addition, we found that CHOP is expressed in the subventricular zone (SVZ) and striatum of the young adult mouse brain. The number of apoptotic cells in the SVZ decreased in chop(-/-) mice. These results indicate that the ER stress-CHOP pathway plays a role in neuronal apoptosis during the development of the brain.
Tajiri
Seiji
S
Department of Molecular Genetics, Graduate School of Medical Sciences, Kumamoto University, Japan.
Yano
Shigetoshi
S
Morioka
Motohiro
M
Kuratsu
Jun-ichi
J
Mori
Masataka
M
Gotoh
Tomomi
T
D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D017931:DNA Primers; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051379:Mice; D009414:Nerve Growth Factors / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051742:Transcription Factor CHOP / Q000502:physiology*
D017931:DNA Primers; D009414:Nerve Growth Factors; D051742:Transcription Factor CHOP