FLO11 mediated filamentous growth of the yeast Saccharomyces cerevisiae depends on the expression of the ribosomal RPS26 genes.
The RPS26A and RPS26B isogenes of Saccharomyces cerevisiae encode two almost identical proteins of the small 40S ribosomal subunit, which differ by only two amino acid residues. Growth of an rps26BDelta mutant strain is normal, whereas an rps26ADelta strain displays a reduced growth rate and increased sensitivity towards the specific translational inhibitor paromomycin. An rps26ADelta rps26BDelta double mutant strain is inviable. RPS26A but not RPS26B is required for haploid adhesive and diploid pseudohyphal growth mediated by FLO11, which encodes an adhesion. The RPS26A and RPS26B transcripts make up about 70 and 30% of the cellular RPS26 mRNA, respectively. Overexpression of RPS26B, as well as an RPS26B open reading frame driven by the RPS26A promoter, complements the rps26ADelta deletion and restores haploid invasive growth as well as diploid pseudohyphal growth. These results suggest that the two proteins are functionally interchangeable. FLO11-lacZ activity is not present in haploid rps26ADelta yeast mutant strains, even though FLO11 mRNA levels are not reduced. This suggests that the amount of Rps26p is critical for accurate translation of the FLO11 mRNA, and therefore for the dimorphic switch of the bakera9s yeast from a single cell yeast to an adhesive filamentous growth form.
Strittmatter
Axel W
AW
Institute of Microbiology and Genetics, Georg-August-Universität, 37077, Göttingen, Germany.
Fischer
Claudia
C
Kleinschmidt
Malte
M
Braus
Gerhard H
GH
D000595:Amino Acid Sequence / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002448:Cell Adhesion / Q000235:genetics; D004171:Diploidy; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000502:physiology*; D006238:Haploidy; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D010303:Paromomycin / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D014176:Protein Biosynthesis / Q000187:drug effects / Q000235:genetics; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012331:RNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics; D017384:Sequence Deletion
D000900:Anti-Bacterial Agents; C110690:FLO11 protein, S cerevisiae; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D020033:Protein Isoforms; D012331:RNA, Fungal; C489204:RPS26A protein, S cerevisiae; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D010303:Paromomycin