[The role of quarternary structure in fluorescent protein stability].
The stability of fluorescent proteins (FPs) is of great importance for their use as reporters in studies of gene expression, protein dynamics and localization in cell. A comparative analysis of conformational stability of fluorescent proteins, having different association state was done. The list of studied proteins includes EGFP (monomer of green fluorescent protein, GFP), zFP506 (tetramer GFP), mRFP1 and "dimer2" (monomer and dimmer of red fluorescent protein), DsRed1 (red tetramer). The character of fluorescence intensity changes induced by guanidine hydrochloride (GdnHCl) of these proteins differs significantly. Green tetramer zFP506 has been shown to be more stable than green monomer EGFP, red dimmer "dimer2" has been shown to be less stable than red tetramer DsRed1, while red monomer mRFP1 has been shown to be practically as stable as tetramer DsRedl. It is concluded that the quaternary structure, being an important stabilizing factor, does not represent the only circumstance dictating the dramatic variations between fluorescent proteins in their conformational stability.
Stepanenko
Olesia V
OV
Verkhusha
V V
VV
Shavlovskiĭ
M M
MM
Aleĭnikova
T D
TD
Uverskiĭ
V N
VN
Kuznetsova
I M
IM
Turoverov
K K
KK
D000595:Amino Acid Sequence; D019281:Dimerization; D004926:Escherichia coli / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism*; D019791:Guanidine / Q000494:pharmacology; D008164:Luminescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D011487:Protein Conformation; D020836:Protein Structure, Quaternary / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D000097573:Red Fluorescent Protein
D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D019791:Guanidine; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D000097573:Red Fluorescent Protein; C485184:enhanced green fluorescent protein; C413663:fluorescent protein 583