title: New SNPs in the IGF2 gene and association between this gene and backfat thickness and lean meat content in Large White pigs.
abstract: IGF2-in3-G3072A is a causative mutation for paternally expressed quantitative trait loci on the p arm of porcine chromosome 2 with substantial effect on muscle growth and backfat thickness. The linkage disequilibrium between IGF2-in3-G3072A and IGF2-in7-G162C (IGF2-NciI) in four breeds and associations between these polymorphisms and growth and meat performance in pigs of the Large White breed were analysed. A significant effect of these polymorphisms on backfat thickness and lean meat content was found. In addition, we identified two new single nucleotide polymorphisms (SNPs) in intron 7 of the gene. The existence of complete linkage disequilibrium between IGF2-in3-G3072A locus in the population under study where the locus segregated and SNPs in intron 7 of the IGF2 gene detectable with simple and reliable polymerase chain reaction-restriction fragment length polymorphism techniques (G162C, C179G and G186T) offer possibilities to use these SNPs for genotyping of quantitative trait nucleotide in Large White and Landrace breeds.
authors.affiliation: Department of Morphology, Physiology and Genetics of Animals, Mendel University of Agriculture and Forestry Brno, Brno, Czech Republic. zuzana.vykoukalova@quick.cz
authors.forename: Z
authors.initials: Z
authors.lastname: Vykoukalová
authors.forename: A
authors.initials: A
authors.lastname: Knoll
authors.forename: J
authors.initials: J
authors.lastname: Dvorák
authors.forename: S
authors.initials: S
authors.lastname: Cepica
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D007335:Insulin-Like Growth Factor II; D015810:Linkage Disequilibrium; D008297:Male; D008460:Meat / Q000592:standards*; D009154:Mutation / Q000235:genetics / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D040641:Quantitative Trait Loci / Q000235:genetics / Q000502:physiology*; D013552:Swine / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D017931:DNA Primers; C035170:IGF2 protein, human; D011506:Proteins; D007335:Insulin-Like Growth Factor II