Alpha-galactosidase production by Aspergillus oryzae in solid-state fermentation.
Comparisons were made for alpha-galactosidase production using red gram plant waste (RGPW) with wheat bran (WB) and other locally available substrates using the fungus Aspergillus oryzae under solid-state fermentation (SSF). RGPW proved to be potential substrate for alpha-galactosidase production as it gave higher enzyme titers (3.4 U/g) compared to WB (2.7 U/g) and other substrates tested. Mixing WB with RGPW (1:1, w/w) resulted enhanced alpha-galactosidase yield. The volume of moistening agent in the ratio of 1:2 (w/v), pH 5.5 and 1 ml (1 x 10(6) spores) of inoculum volume and four days incubation were optimum for alpha-galactosidase production. Increase in substrate concentration (RGPW+WB) did not decrease enzyme yield in trays.
Shankar
S K
SK
Department of Biochemistry, Gulbarga University, Gulbarga, 585106, Karnataka, India.
Mulimani
V H
VH
D001236:Aspergillus oryzae / Q000201:enzymology*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D000519:alpha-Galactosidase / Q000096:biosynthesis* / Q000378:metabolism
D000519:alpha-Galactosidase