Pinelliae Rhizoma herbal-acupuncture solution induced apoptosis in human cervical cancer cells, SNU-17.
Pinelliae Rhizoma has been used traditionally in Korea to promote the liver Qi activity and the function of the digestive system. We investigated whether the Pinelliae Rhizoma herbal-acupuncture solution (PRHS) would induce cell-death on SNU-17, human cervical cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to investigate the cytotoxicity of PRHS. The cell death was identified as apoptosis with 4, 6-diamidineo-2-phenylindole (DAPI) staining, and terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. PRHS could induce apoptosis of SNU-17 via Bax-related caspase-3 activation. The expressions of both Bax, a pro-apoptotic gene, and caspase-3, an apoptotic gene, were increased. The results might provide the experimental data for the clinical use of Pinelliae Rhizoma on cervical cancer.
Yoon
Jong-Seok
JS
Department of Acupuncture and Moxibustion, College of Oriental Medicine, Daegu Haany University, Daegu, 730-090, Korea.
Seo
Jung-Chul
JC
Han
Sang-Won
SW
D026881:Acupuncture; D017209:Apoptosis; D001483:Base Sequence; D045744:Cell Line, Tumor; D017931:DNA Primers; D005260:Female; D029001:Herbal Medicine; D006801:Humans; D020287:In Situ Nick-End Labeling; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012996:Solutions; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000628:therapy*
D017931:DNA Primers; D012333:RNA, Messenger; D012996:Solutions