Measuring D-serine efflux from mouse cortical brain slices using online microdialysis-capillary electrophoresis.
Efflux of a number of important neurochemicals, including D-serine, L-serine, taurine, glutamate, and gamma-aminobutyric acid (GABA), from mouse cortical brain slices housed in a 7 microL perfusion chamber was monitored using online microdialysis-CE (MD-CE). Analyte concentrations could be measured every 20-27 s using the MD-CE instrument. Stimulation with high potassium induced increased release of D-serine. Kainic acid (KA) induced D-serine release, but this release was not blocked by 6-cyano-7-nitroquinoxaline-2,3-dione, suggesting that alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid/KA receptors do not mediate D-serine release. Application of L-serine, the precursor of D-serine, resulted in increased extracellular D-serine concentrations. L-Cysteine also increased extracellular D-serine levels in a partially Na+-dependent manner. The observed effects upon application of L-serine and L-cysteine support the involvement of ASC neutral amino acid transporters in regulating the extracellular concentration of D-serine concentration through competitive inhibition of uptake or increased release through heteroexchange.
O'Brien
Kylie B
KB
Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Bowser
Michael T
MT
D000818:Animals; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods*; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017551:Microdialysis / Q000379:methods*; D009862:Online Systems; D010477:Perfusion; D011188:Potassium / Q000494:pharmacology; D018091:Receptors, AMPA / Q000187:drug effects / Q000378:metabolism; D018092:Kainic Acid Receptors / Q000187:drug effects / Q000378:metabolism; D012694:Serine / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism
D007608:Kainic Acid; D011188:Potassium; D018091:Receptors, AMPA; D018092:Kainic Acid Receptors; D012694:Serine