title: Expression of tumor endothelial marker 7 mRNA and protein in the dorsal root ganglion neurons of the rat.
abstract: Tumor endothelial marker7 (TEM7) is a transmembrane protein that is highly expressed in the tumor endothelium and neurons. In the present study, the expression profile of TEM7 mRNA and protein was investigated in the dorsal root ganglion (DRG) of the rats. In situ hybridization and reverse transcription polymerase chain reaction studies revealed that TEM7 mRNA expression was localized to the neuronal population of the sensory ganglion. Immunohistochemical analysis on TEM protein with specific antibodies further supported the spatial expression patterns of TEM7 mRNA in the DRG. The temporal expression of TEM7 mRNA in the DRG demonstrated a postnatal increase of TEM7 mRNA expression. Our results indicate that TEM7 may play a role in the peripheral sensory neurons of the vertebrate nervous systems.
authors.affiliation: Department of Physiology, College of Medicine, Dong-A University, 3-1, Dongdaesin-Dong, Seo-Gu, Busan 602-714, South Korea.
authors.forename: Hyun Kyoung
authors.initials: HK
authors.lastname: Lee
authors.forename: Dong Sik
authors.initials: DS
authors.lastname: Kang
authors.forename: In Ae
authors.initials: IA
authors.lastname: Seo
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Choi
authors.forename: Hwan Tae
authors.initials: HT
authors.lastname: Park
authors.forename: Joo In
authors.initials: JI
authors.lastname: Park
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004622:Embryo, Mammalian; D005727:Ganglia, Spinal / Q000166:cytology*; D015870:Gene Expression / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C502423:Plxdc1 protein, rat; D012333:RNA, Messenger