Genesis of and trafficking to the Maurer's clefts of Plasmodium falciparum-infected erythrocytes.
Malaria parasites export proteins beyond their own plasma membrane to locations in the red blood cells in which they reside. Maurer's clefts are parasite-derived structures within the host cell cytoplasm that are thought to function as a sorting compartment between the parasite and the erythrocyte membrane. However, the genesis of this compartment and the signals directing proteins to the Maurer's clefts are not known. We have generated Plasmodium falciparum-infected erythrocytes expressing green fluorescent protein (GFP) chimeras of a Maurer's cleft resident protein, the membrane-associated histidine-rich protein 1 (MAHRP1). Chimeras of full-length MAHRP1 or fragments containing part of the N-terminal domain and the transmembrane domain are successfully delivered to Maurer's clefts. Other fragments remain trapped within the parasite. Fluorescence photobleaching and time-lapse imaging techniques indicate that MAHRP1-GFP is initially trafficked to isolated subdomains in the parasitophorous vacuole membrane that appear to represent nascent Maurer's clefts. The data suggest that the Maurer's clefts bud from the parasitophorous vacuole membrane and diffuse within the erythrocyte cytoplasm before taking up residence at the cell periphery.
Spycher
Cornelia
C
Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, Socinstrasse 57, CH 4002 Basel, Switzerland.
Rug
Melanie
M
Klonis
Nectarios
N
Ferguson
David J P
DJ
Cowman
Alan F
AF
Beck
Hans-Peter
HP
Tilley
Leann
L
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000648:ultrastructure; D002465:Cell Movement; D004912:Erythrocytes / Q000166:cytology* / Q000469:parasitology*; D005091:Exons / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000502:physiology*; D015800:Protozoan Proteins / Q000737:chemistry / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism
D002352:Carrier Proteins; C478484:MAHRP-1 protein, Plasmodium falciparum; D008565:Membrane Proteins; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins