[Usefulness of NCI-N87 cell lines in Helicobacter pylori infected gastric mucosa model].
OBJECTIVE
The unavailability of human gastric cell lines representative of the normal gastric epithelial function such as polarized monolayer restricts the application of cell culture system in approaching the field of Helicobacter pylori (H. pylori) infected gastric mucosa models. The present investigation aimed at assessing the usefulness of NCI-N87 cell line as an adequate cellular model to study the pathophysiology of human H. pylori infection.


METHODS
For the identification of epithelial phenotypes at low magnification, cells were observed on a phase-contrast microscope and confocal microscope. Transepithelial resistance (TER) was measured on NCI-N87 cells seeded on Transwell to identify monolayer polarity two or three times a week after confluency. The IL-8 level was determined by ELISA at 24 hours after the administration of HP60190 and IL-1 alpha on NCI-N87 cells. IL-8 level was compared in both upper and lower well with the control.


RESULTS
A monolayer phenotype was observed in NCI-N87 cell lines by using confocal microscope. TER was measured as 400-500 (Omega x cm2) at two or three weeks after cell culture. In NCI-N87 cell lines, IL-8 level was significantly increased after 24 hour compared to control, and was prominent in the lower well.


CONCLUSIONS
These results suggest that NCI-N87 cell line may be useful in H. pylori infected gastric mucosa model.
Jung
Yong Jin
YJ
Department of Internal Medicine, Seoul National University Boramae Hospital, Seoul National University College of Medicine, Seoul, Korea.
Lee
Kook Lae
KL
Kim
Byung Kwan
BK
Kim
Ji Won
JW
Jeong
Ji Bong
JB
Kim
Sang Gyun
SG
Kim
Joo Sung
JS
Jung
Hyun Chae
HC
Song
In Sung
IS
D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D016481:Helicobacter Infections / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D018613:Microscopy, Confocal; D008858:Microscopy, Phase-Contrast; D010641:Phenotype
D016209:Interleukin-8