Characterization of targeted mouse embryonic stem cell chromosomes: karyotyping and fluorescence in situ hybridization of metaphase spreads.
The manipulation of genes in mouse embryonic stem (ES) cells can result in chromosome abnormalities. This chapter describes methods for karyotyping of the manipulated ES cell line before injection into blastocysts and the use of fluorescence in situ hybridization to confirm the deletion of a targeted gene. The method is illustrated by describing how an ES cell line targeted for the deletion of Fmo genes was characterized.
Hernandez
Diana
D
Department of Biochemistry and Molecular Biology, University College London, UK.
Phillips
lan R
lR
Shephard
Elizabeth A
EA
D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008677:Metaphase; D051379:Mice; D013234:Stem Cells / Q000648:ultrastructure*