[Establishment of a rat model of alcoholic liver fibrosis induced by complex factors].
OBJECTIVE
To establish a model of alcoholic liver fibrosis (ALF) in rats induced by complex factors.


METHODS
Forty-seven healthy male rats were divided into three groups: normal control group (n=12), minor CCl4 group (n=12) and complex factors group (n=27). The rats in the complex factors group were fed a complex diet including alcohol, corn oil and pyrazole, and administered with intraperitoneal injection of minor CCl4 to induce ALF. During induction process, the histopathological changes of liver tissue and the values of liver-to-body weight ratio were both observed regularly. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyltransferase (gamma-GT) in these three groups were all examined at the 12th week of the induction process.


RESULTS
At the 12th week of the induction process, the model of ALF induced by complex factors was successfully established in rats, and the histopathological presentations showed alcoholic fatty liver, hepatitis and liver fibrosis in a sequence along with the induction process. The value of liver-to-body weight ratio and the serum levels of ALT, AST and gamma-GT of rats in the complex factors group were all significantly different from those in the other two groups.


CONCLUSIONS
It is a steady and effective way to induce ALF in rats with complex diet and minor CCI4 injection.
Wang
Lei
L
Laboratory of Fatty Liver Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Ji
Guang
G
Zheng
Pei-Yong
PY
Long
Ai-Hua
AH
D000818:Animals; D002252:Carbon Tetrachloride Poisoning; D004195:Disease Models, Animal; D000431:Ethanol; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000431:Ethanol