title: Examination of peripheral blood films using automated microscopy; evaluation of Diffmaster Octavia and Cellavision DM96.
abstract: BACKGROUND
  Differential counting of peripheral blood cells is an important diagnostic tool. Yet, this technique requires highly trained staff, is labour intensive and has limited statistical reliability. A recent development in this field was the introduction of automated peripheral blood differential counting systems. These computerised systems provide an automated morphological analysis of peripheral blood films, including a preclassification of both red and white cells (RBCs and WBCs, respectively).
  
  
  OBJECTIVE
  To investigate the ability of two automated microscopy systems to examine peripheral blood smears.
  
  
  METHODS
  Two automated microscopy systems, the Cellavision Diffmaster Octavia (Octavia) and Cellavision DM96 (DM96), were evaluated.
  
  
  RESULTS
  The overall preclassification accuracy values for the Octavia and the DM96 systems were 87% and 92%, respectively. Evaluation of accuracy (WBC analysis) showed good correlation for both automated systems when compared with manual differentiation. Total analysis time (including post classification) was 5.4 min/slide for the Octavia and 3.2 min/slide for the DM96 (100 WBC/slide) system. The DM96 required even less time than manual differentiation by an experienced biomedical scientist.
  
  
  CONCLUSIONS
  The Octavia and the DM96 are automated cell analysis systems capable of morphological classification of RBCs and WBCs in peripheral blood smears. Classification accuracy depends on the type of pathological changes in the blood sample. Both systems operate most effectively in the analysis of non-pathological blood samples.
authors.affiliation: Department of Clinical Chemistry, Albert Schweitzer Ziekenhuis, Dordrecht, The Netherlands. h.ceelie@asz.nl
authors.forename: H
authors.initials: H
authors.lastname: Ceelie
authors.forename: R B
authors.initials: RB
authors.lastname: Dinkelaar
authors.forename: W
authors.initials: W
authors.lastname: van Gelder
mesh_terms: D001772:Blood Cell Count / Q000295:instrumentation* / Q000379:methods; D001800:Blood Specimen Collection / Q000295:instrumentation* / Q000379:methods; D004906:Erythrocyte Count / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007958:Leukocyte Count / Q000295:instrumentation / Q000379:methods; D007962:Leukocytes / Q000145:classification; D015203:Reproducibility of Results; D013996:Time and Motion Studies