Stable isotope metabolic labeling of Neisseria meningitidis lipooligosaccharide.
The lipooligosaccharide (LOS) of a Neisseria meningitidis acetate auxotroph was metabolically labeled with either [2-13C]-sodium acetate or [1,2-13C2]-sodium acetate. In this study, we demonstrated that this label was efficiently incorporated into both the lipid A acyl moieties and the two N-acetylglucosamines present in the oligosaccharide branch of the LOS. The development of this efficient labeling protocol should prove useful in future structural studies analyzing the interactions between LOS and host proteins.
Post
Deborah M B
DM
The Buck Institute for Age Research, Novato, California 94945, USA.
Zhang
Desheng
D
Weiss
Jerrold P
JP
Gibson
Bradford W
BW
D000085:Acetates / Q000737:chemistry; D002250:Carbon Radioisotopes; D004731:Endotoxins / Q000737:chemistry*; D007553:Isotope Labeling; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D013058:Mass Spectrometry; D008970:Molecular Weight; D009345:Neisseria meningitidis / Q000737:chemistry* / Q000254:growth & development; D021241:Spectrometry, Mass, Electrospray Ionization
D000085:Acetates; D002250:Carbon Radioisotopes; D004731:Endotoxins; D008050:Lipid A; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides