Characterization of monoclonal antibodies directed against the canine distemper virus nucleocapsid protein.
We have established four monoclonal antibodies (MAbs) against the nucleocapsid protein (NP) of canine distemper virus (CDV). A competitive binding assay has revealed that the MAbs are directed against two antigenic domains. An immunofluorescence assay using a series of deletion clones of the NP and an immunoprecipitation assay using the NP have revealed that two of the MAbs recognize the C-terminal region of the NP while the other two recognize the tertiary structure of the N-terminal domain. These MAbs reacted with all eight strains of CDV used in this study, but showed different reactivities against measles virus and rinderpest virus.
Masuda
Munemitsu
M
Department of Biochemistry, School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Sato
Hiroki
H
Kamata
Hiroshi
H
Katsuo
Tomoe
T
Takenaka
Akiko
A
Miura
Ryuichi
R
Yoneda
Misako
M
Tsukiyama-Kohara
Kyoko
K
Mizumoto
Kiyohisa
K
Kai
Chieko
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D000918:Antibody Specificity; D000940:Antigenic Variation; D001667:Binding, Competitive; D019556:COS Cells; D002522:Chlorocebus aethiops; D004216:Distemper / Q000821:virology; D004217:Distemper Virus, Canine / Q000276:immunology*; D004285:Dogs; D018604:Epitope Mapping; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D008807:Mice, Inbred BALB C; D016254:Mutagenesis, Insertional; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D015531:Radioimmunoprecipitation Assay; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D019590:Nucleocapsid Proteins; D012367:RNA, Viral