Maltose and maltodextrin utilization by Bacillus subtilis.
Bacillus subtilis can utilize maltose and maltodextrins that are derived from polysaccharides, like starch or glycogen. In this work, we show that maltose is taken up by a member of the phosphoenolpyruvate-dependent phosphotransferase system and maltodextrins are taken up by a maltodextrin-specific ABC transporter. Uptake of maltose by the phosphoenolpyruvate-dependent phosphotransferase system is mediated by maltose-specific enzyme IICB (MalP; synonym, GlvC), with an apparent K(m) of 5 microM and a V(max) of 91 nmol . min(-1) . (10(10) CFU)(-1). The maltodextrin-specific ABC transporter is composed of the maltodextrin binding protein MdxE (formerly YvdG), with affinities in the low micromolar range for maltodextrins, and the membrane-spanning components MdxF and MdxG (formerly YvdH and YvdI, respectively), as well as the energizing ATPase MsmX. Maltotriose transport occurs with an apparent K(m) of 1.4 microM and a V(max) of 4.7 nmol . min(-1) . (10(10) CFU)(-1).
Schönert
Stefan
S
Lehrstuhl für Mikrobiologie, Fachbereich Biologie der Universität Konstanz, Universitätsstrasse 10, M605, D-78457 Konstanz, Germany. stefan.schoenert@gmx.de
Seitz
Sabine
S
Krafft
Holger
H
Feuerbaum
Eva-Anne
EA
Andernach
Iris
I
Witz
Gabriele
G
Dahl
Michael K
MK
D001412:Bacillus subtilis / Q000378:metabolism*; D001692:Biological Transport; D005838:Genotype; D007700:Kinetics; D008320:Maltose / Q000378:metabolism*; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010957:Plasmids; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D015183:Restriction Mapping
D011134:Polysaccharides; D008320:Maltose; C008315:maltodextrin; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System