title: A comparative study of 99mTc-YIGSR and 99mTc-MIBI uptake in tumor cells.
abstract: To investigate a new kind of tumor tracer 99mTc-YIGSR developed from a five amino structure (YIGSR) of the Laminin -chain, which can bind to the laminin receptors of tumor specifically, and radiolabeled with MAG3. (1) Preparation of the 99mTc-YIGSR probe: with S-Acetly-NH3-MAG3 as the chelator and with proper reductants YIGSR was labeled with 99mTc; (2) Cell culture and viability measurement: EAC was maintained in RPMI 1640 supplemented with calf serum; the trypan blue exclusion was applied to calculate the cell viability; (3) Study of the cell dynamic: The EAC's uptake of 99mTc-YIGSR and 99mTc-MIBI was observed at 37 degrees C and 22 degrees C, respectively. (1) The labeling efficiencies of 99mTc-YIGSR and 99mTc-MIBI were (62 +/- 3)% and (96 +/- 2)%, respectively; (2) The cell viability was declined with time of incubation; (3) At 37 degrees C, the EAC'S uptake of 99mTc-YIGSR and 99mTc-MIBI reached the peak of (43.16 +/- 2.4) % and (24.4 +/- 1.8) % at 60 min, respectively; and at 22 degrees C, the highest uptake was (26.5 +/- 2.1) % and (9.47 +/- 1.9) % at 60 min, respectively. The in vitro study suggests that 99mTc-YIGSR is superior to 99mTc-MIBI in cell uptake and has potential value in tumor imaging.
authors.affiliation: Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Hu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Lan
authors.forename: Yongxue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zairong
authors.initials: Z
authors.lastname: Gao
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000000981:diagnostic imaging*; D005260:Female; D007797:Laminin; D009842:Oligopeptides; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D051381:Rats; D017263:Technetium Tc 99m Mertiatide / Q000493:pharmacokinetics*; D017256:Technetium Tc 99m Sestamibi / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D007797:Laminin; D009842:Oligopeptides; D019275:Radiopharmaceuticals; C121346:laminin 1; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; D017263:Technetium Tc 99m Mertiatide; D017256:Technetium Tc 99m Sestamibi