title: [Detection of MSH2 gene mutation by PCR].
abstract: OBJECTIVE
  To establish a genetic diagnosis method for a novel MSH2 mutation.
  
  
  METHODS
  A specific primer on the mutated site of MSH2 was synthesized and PCR was conducted using the specific primer and another downstream primer. PCR products were electrophoresed and then the carriers with the novel gene mutation of the carriers or non-carriers were identified.
  
  
  RESULTS
  MSH2 in a hereditary nonpolyposis colorectal cancer family were successfully found.
  
  
  CONCLUSIONS
  The method is effective and simple for genetic diagnosis of the novel mutation in MSH2.
authors.affiliation: School of Biologic Science and Technology, Central South University, Changsha, China. zhengduo@hotmail.com
authors.forename: Duo
authors.initials: D
authors.lastname: Zheng
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Liu
authors.forename: Tie-gang
authors.initials: TG
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Tang
authors.forename: Wei-xin
authors.initials: WX
authors.lastname: Hu
mesh_terms: D001483:Base Sequence; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D051718:MutS Homolog 2 Protein / Q000235:genetics*; D010375:Pedigree; D017354:Point Mutation; D016133:Polymerase Chain Reaction
chemical_list: D051718:MutS Homolog 2 Protein