Plasma membrane destination of the classical Xenopus laevis progesterone receptor accelerates progesterone-induced oocyte maturation.
Xenopus laevis oocyte maturation is induced by the steroid hormone progesterone through a non-genomic mechanism initiated at the cell membrane. Recently, two Xenopus oocyte progesterone receptors have been cloned; one is the classical progesterone receptor (xPR-1) involved in genomic actions and the other a putative seven-transmembrane-G-protein-couple receptor. Both receptors are postulated to be mediating the steroid-induced maturation process in the frog oocyte. In this study, we tested the hypothesis that the classical progesterone receptor, associated to the oocyte plasma membrane, is participating in the reinitiation of the cell cycle. Addition of a myristoilation and palmytoilation signal at the amino terminus of xPR-1 (mp xPR-1), increased the amount of receptor associated to the oocyte plasma membrane and most importantly, significantly potentiated progesterone-induced oocyte maturation sensitivity. These findings suggest that the classical xPR-1, located at the plasma membrane, is mediating through a non-genomic mechanism, the reinitiation of the meiotic cell cycle in the X. laevis oocyte.
Martinez
Silvana
S
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Casilla 160-C, Universidad de Concepción, Concepción, Chile.
Grandy
Rodrigo
R
Pasten
Pamela
P
Montecinos
Hernán
H
Montecino
Martín
M
Olate
Juan
J
Hinrichs
María Victoria
MV
D000818:Animals; D019556:COS Cells; D002453:Cell Cycle / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism*; D002522:Chlorocebus aethiops; D005260:Female; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011374:Progesterone / Q000378:metabolism / Q000494:pharmacology*; D011499:Protein Processing, Post-Translational; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
D011980:Receptors, Progesterone; C419774:XPR-1 protein, Xenopus; D029867:Xenopus Proteins; D011374:Progesterone