title: Exonuclease requirements for recombination of lambda-phage in recD mutants of Escherichia coli.
abstract: Recombination of lambda red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5'-3' exonuclease activity of RecJ and exonuclease VII is essential for lambda-recombination, whereas exonucleases of 3'-5' polarity are dispensable. The implications of the presented data on current models for recombination initiation in E. coli are discussed.
authors.affiliation: Division of Molecular Biology, Ruder Bosković Institute, 10002 Zagreb, Croatia. dermic@irb.hr
authors.forename: Damir
authors.initials: D
authors.lastname: Dermić
authors.forename: Davor
authors.initials: D
authors.lastname: Zahradka
authors.forename: Mirjana
authors.initials: M
authors.lastname: Petranović
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000821:virology; D029968:Escherichia coli Proteins / Q000235:genetics*; D043211:Exodeoxyribonuclease V / Q000235:genetics*; D008957:Models, Genetic; D009154:Mutation; D011995:Recombination, Genetic
chemical_list: D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli