Effect of Mouriri pusa extracts on experimentally induced gastric lesions in rodents: role of endogenous sulfhydryls compounds and nitric oxide in gastroprotection.
Several plants are used in folk medicine to treat gastrointestinal disorders. Mouriri pusa Gardn. (Melastomataceae) is a medicinal plant commonly used in the central region of Brazil against gastric ulcer. Two organic extracts methanolic (MeOH) and dichloromethane (DCM) obtained by sequential extraction from the leaves of Mouriri pusa were evaluated for their ability to protect the gastric mucosa against injuries caused by necrotizing agents (0.3M HCl/60% EtOH, absolute ethanol, non-steroidal anti-inflammatory drug, stress and pylorus ligature) in mice and rats. The best results were obtained after pretreatment with MeOH extract whereas the DCM extract did not show the same significant antiulcerogenic activity. No acute toxicity was observed in animals treated with 5 g/kg, p.o. of MeOH extract. The mechanism involving the antiulcerogenic action of MeOH extract seemed to be related to NO generation and also suggested the effective participation of endogenous sulfhydryl group in the gastroprotective action. Phytochemical investigation of the MeOH extract of Mouriri pusa yielded tannins, flavonoids and (-)-epicatechin. The presence of these phenolic compounds probably would explain the antiulcerogenic effect of the polar extract of Mouriri pusa leaves.
Andreo
Marcio Adriano
MA
Departamento de Química Orgânica, Instituto de Química, CP 355, CEP 14801-970, UNESP, São Paulo State University, Araraquara, SP, Brasil.
Ballesteros
Kátia Verônica Rodríguez
KV
Hiruma-Lima
Clélia Akiko
CA
Machado da Rocha
Lúcia Regina
LR
Souza Brito
Alba Regina Monteiro
AR
Vilegas
Wagner
W
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D001938:Brazil; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000431:Ethanol; D005033:Ethylmaleimide / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D005745:Gastric Acidity Determination; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D005769:Gastrointestinal Motility / Q000187:drug effects; D006851:Hydrochloric Acid; D008297:Male; D008519:Medicine, Traditional; D035322:Melastomataceae; D051379:Mice; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves; D011708:Pylorus / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D013312:Stress, Physiological / Q000150:complications; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D009569:Nitric Oxide; D000431:Ethanol; D019001:Nitric Oxide Synthase; D005033:Ethylmaleimide; D006851:Hydrochloric Acid; D019331:NG-Nitroarginine Methyl Ester