Influence of diets supplemented with vitamins C and E on pirarucu (Arapaima gigas) blood parameters.
This study evaluated the influence of diets supplemented with 500, 800, 1200 mg kg-1 of vitamin C (ascorbic acid or AA) and vitamin E (alpha-tocopherol or alpha-T) on the physiological responses of pirarucu fed for 2 months. Weight and mortality were not affected by dietary vitamin type or their concentrations. Significant increase (p<0.05) on the red blood cells count was obtained on treatments with 800 and 1200 mg AA kg-1 and on the hemoglobin concentration on treatment with 500 mg alpha-T kg-1 relatively to control. Mean corpuscular volume presented a significant decrease (p<0.05) on treatment with 800 and 1200 mg AA kg-1 when compared to control. Mean corpuscular hemoglobin concentration was significantly high (p<0.05) on treatment with 500 mg alpha-T kg-1. Only in vitamin C treatments, we noticed a significant increase (p<0.05) in the number of leucocytes relative to control. All fish in the vitamin-supplemented treatments, except 500 mg AA kg-1, had high total protein values compared to control. Fish treated with 800 or 1200 mg alpha-T kg-1 also showed increases in plasma glucose concentrations. Our results suggest that 800 and 1200 mg AA kg-1 are probably the most suitable concentrations for pirarucu diets, although high vitamin E diets are not necessary for quantitative leucocyte increases for this species.
de Andrade
Jaqueline Inês Alves
JI
Aquaculture Department, INPA (Instituto Nacional de Pesquisas da Amazônia), Av. André Araújo, 2936, P.O. Box 478, Manaus, AM, 69060-001, Brazil.
Ono
Eduardo Akifumi
EA
de Menezes
Glauber Cruz
GC
Brasil
Elenice Martins
EM
Roubach
Rodrigo
R
Urbinati
Elisabeth Criscuolo
EC
Tavares-Dias
Marcos
M
Marcon
Jaydione Luiz
JL
Affonso
Elizabeth Gusmão
EG
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001769:Blood / Q000187:drug effects*; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004906:Erythrocyte Count; D005398:Fisheries; D005399:Fishes / Q000502:physiology*; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D007958:Leukocyte Count; D013312:Stress, Physiological; D014810:Vitamin E / Q000494:pharmacology*
D001786:Blood Glucose; D006454:Hemoglobins; D014810:Vitamin E; D001205:Ascorbic Acid