title: Multicenter quality control of the detection of HIV-1 genome in semen before medically assisted procreation.
abstract: Couples in whom the man is HIV-1-positive may use medically assisted procreation in order to conceive a child without contaminating the female partner. But, before medically assisted procreation, the semen has to be processed to exclude HIV and tested for HIV nucleic acid before and after processing. The performance was evaluated of the technical protocols used to detect and quantify HIV-1 in 11 centers providing medically assisted procreation for couples with HIV-1 infected men by testing panels of seminal plasma and cells containing HIV-1 RNA and/or DNA. The performance of these tests varied due to the different assays used. False positive results were obtained in 14-19% of cases. The sensitivity for RNA detection in seminal plasma was 500-1,000 RNA copies/ml, over 500 RNA copies/10(6) cells in semen cells, and for DNA detection in semen cells 50-500 DNA copies/10(6) cells. The use of silica-based extraction seemed to increase the assay performance, whereas the use of internal controls to detect PCR inhibitor did not. This first quality control highlights the need for technical improvements of the assays to detect and quantify HIV in semen fractions and for regular evaluation of their performance.
authors.affiliation: Laboratoire de Virologie, laboratoire de Spermiologie et CECOS Midi-Pyrénées, Toulouse University Hospital, Toulouse, France. pasquier.c@chu-toulouse.fr
authors.forename: Christophe
authors.initials: C
authors.lastname: Pasquier
authors.forename: Deborah
authors.initials: D
authors.lastname: Anderson
authors.forename: Corinne
authors.initials: C
authors.lastname: Andreutti-Zaugg
authors.forename: Rianne
authors.initials: R
authors.lastname: Baume-Berkenbosch
authors.forename: Florence
authors.initials: F
authors.lastname: Damond
authors.forename: Aviva
authors.initials: A
authors.lastname: Devaux
authors.forename: Yvon
authors.initials: Y
authors.lastname: Englert
authors.forename: Julie
authors.initials: J
authors.lastname: Galimand
authors.forename: Carole
authors.initials: C
authors.lastname: Gilling-Smith
authors.forename: Odile
authors.initials: O
authors.lastname: Guist'hau
authors.forename: Lital
authors.initials: L
authors.lastname: Hollander
authors.forename: Marianne
authors.initials: M
authors.lastname: Leruez-Ville
authors.forename: Benoit
authors.initials: B
authors.lastname: Lesage
authors.forename: Anne
authors.initials: A
authors.lastname: Maillard
authors.forename: Anne-geneviève
authors.initials: AG
authors.lastname: Marcelin
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Schmitt
authors.forename: Augusto
authors.initials: A
authors.lastname: Semprini
authors.forename: Maria
authors.initials: M
authors.lastname: Vourliotis
authors.forename: Chong
authors.initials: C
authors.lastname: Xu
authors.forename: Louis
authors.initials: L
authors.lastname: Bujan
mesh_terms: D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D011786:Quality Control; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D027724:Reproductive Techniques, Assisted / Q000592:standards*; D012661:Semen / Q000821:virology*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral