title: Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA.
abstract: Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNA(Tyr). The crystals belong to a tetragonal space group P4(1)2(1)2 with cell dimensions of a = b = 63.85 Angstrom, and c = 330.3 Angstrom. The asymmetric unit contains one molecule each of yTyrRS and tRNA(Tyr) (one-half of a 2:2 complex). X-ray diffraction data have been collected up to 2.5 Angstrom resolution.
authors.affiliation: School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kusakabe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohno
authors.forename: Nobutada
authors.initials: N
authors.lastname: Tanaka
authors.forename: Masashi
authors.initials: M
authors.lastname: Nakamura
authors.forename: Masaru
authors.initials: M
authors.lastname: Tsunoda
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Moriguchi
authors.forename: Norio
authors.initials: N
authors.lastname: Asai
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokogawa
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nishikawa
authors.forename: Kazuo T
authors.initials: KT
authors.lastname: Nakamura
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D012365:RNA, Transfer, Tyr / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014448:Tyrosine-tRNA Ligase / Q000737:chemistry*
chemical_list: D012365:RNA, Transfer, Tyr; D014448:Tyrosine-tRNA Ligase