title: The derivation of clinical-grade human embryonic stem cell lines.
abstract: The pluripotent nature of human embryonic stem cells (hESC) has attracted great interest in using them as a source of cells or tissue in cell therapy. However, in order to be used in regenerative medicine, the pluripotent hESC lines should be established and propagated according to good manufacturing practice quality requirements. The cultures should be animal substance free in order to exclude the risk of infections and immunogenity. They should also be genetically and epigenetically normal. The detailed molecular mechanisms of their pluripotency are still not defined. Using human feeder cells, a medium containing only human proteins, the mechanical isolation of the inner cell mass and mechanical passaging of hESC, is a safe option until a functional defined medium containing physiological concentrations of regulatory factors is available.
authors.affiliation: Institute of Regenerative Medicine, Regea, University of Tampere and Tampere University Hospital, FI 33520 Tampere, Finland.
authors.forename: Heli
authors.initials: H
authors.lastname: Skottman
authors.forename: M Sirac
authors.initials: MS
authors.lastname: Dilber
authors.forename: Outi
authors.initials: O
authors.lastname: Hovatta
mesh_terms: D002460:Cell Line; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D013234:Stem Cells / Q000166:cytology*
chemical_list: D003470:Culture Media