title: Cryopreservation of somatic embryos of the herbaceous peony (Paeonia lactiflora Pall.) by air drying.
abstract: This study was carried out to establish a suitable method for the cryopreservation of somatic embryos of the herbaceous peony. The somatic embryos were obtained from cotyledon and anther cultures on a MS medium supplemented with abscisic acid (ABA) and phenylacetic acid (PAA), respectively. The frequency of somatic embryo formation was the greatest (61%) from the cotyledons cultured on a MS medium supplemented with 1.0 mg l(-1) of ABA. Embryos were also obtained directly from anthers cultured on a MS medium with or without 2.0 mg l(-1) of PAA. For the cryopreservation of peony somatic embryos, the embryos were dried under a stream of sterile air and frozen by immersion in liquid nitrogen. Thawed embryos were germinated into plantlets after placing on a medium containing 0.3 mg l(-1) of gibberellic acid (GA(3)). The frequency of the post-thaw regrowth of cryopreserved somatic embryos was related to their size and desiccation time, the latter ranging from 0 to 2 h. When the somatic embryos were desiccated for 1 h, the frequency of post-thaw regrowth was greater than 66%. The frequency of post-thaw regrowth of the cryopreserved somatic embryos from anthers and cotyledon tissues was generally high when they were 2-3 mm in size. Desiccation may be a suitable method for the cryopreservation of somatic embryos of the herbaceous peony.
authors.affiliation: Department of Agronomy, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Hyun Mi
authors.initials: HM
authors.lastname: Kim
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Shin
authors.forename: Jae Keun
authors.initials: JK
authors.lastname: Sohn
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000388:Air; D018548:Cotyledon / Q000196:embryology; D015925:Cryopreservation / Q000379:methods*; D003890:Desiccation / Q000379:methods; D035264:Flowers / Q000196:embryology; D005875:Gibberellins / Q000494:pharmacology; D029596:Paeonia / Q000187:drug effects / Q000196:embryology*; D010648:Phenylacetates / Q000494:pharmacology
chemical_list: D005875:Gibberellins; D010648:Phenylacetates; D000040:Abscisic Acid; C007842:gibberellic acid; C025136:phenylacetic acid