title: Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae.
abstract: DNA mismatches are generated when heteroduplexes formed during recombination involve DNA strands that are not completely complementary. We used tetrad analysis in Saccharomyces cerevisiae to examine the meiotic repair of a base-base mismatch and a four-base loop in a wild-type strain and in strains with mutations in genes implicated in DNA mismatch repair. Efficient repair of the base-base mismatch required Msh2p, Msh6p, Mlh1p, and Pms1p, but not Msh3p, Msh4p, Msh5p, Mlh2p, Mlh3p, Exo1p, Rad1p, Rad27p, or the DNA proofreading exonuclease of DNA polymerase delta. Efficient repair of the four-base loop required Msh2p, Msh3p, Mlh1p, and Pms1p, but not Msh4p, Msh5p, Msh6p, Mlh2p, Mlh3p, Exo1p, Rad1p, Rad27p, or the proofreading exonuclease of DNA polymerase delta. We find evidence that a novel Mlh1p-independent complex competes with an Mlhp-dependent complex for the repair of a four-base loop; repair of the four-base loop was affected by loss of the Mlh3p, and the repair defect of the mlh1 and pms1 strains was significantly smaller than that observed in the msh2 strain. We also found that the frequency and position of local double-strand DNA breaks affect the ratio of mismatch repair events that lead to gene conversion vs. restoration of Mendelian segregation.
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, North Carolina 27710, USA.
authors.forename: Jana E
authors.initials: JE
authors.lastname: Stone
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Petes
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D000619:Aminohydrolases / Q000235:genetics / Q000378:metabolism; D001123:Argininosuccinate Lyase; D020137:Base Pair Mismatch; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000235:genetics / Q000502:physiology*; D004271:DNA, Fungal / Q000737:chemistry; D008540:Meiosis; D051718:MutS Homolog 2 Protein / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D011755:Pyrophosphatases / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D000429:Alcohol Oxidoreductases; C092116:HIS4 protein, S cerevisiae; D000619:Aminohydrolases; D011755:Pyrophosphatases; D051718:MutS Homolog 2 Protein; C087900:Arg4 protein, S cerevisiae; D001123:Argininosuccinate Lyase; D045643:DNA Repair Enzymes