Peptide vector for gene delivery with high affinity for phosphatidylserine.
Since phosphatidylserine (PS) is known to translocate to the external face of the plasma membrane when the cell membrane becomes disordered, we decided to focus our attention on PS as a target molecule for gene delivery. In this paper, the novel peptide Td3701 was designed, synthesized, and characterized for its physico-chemico-biological properties. Td3701 simultaneously exhibited both characters as a DNA carrier and a sensor probe for active targeting, which seemed to be triggered by structural changes in the presence of PS. This is a very unique character among nonviral vectors, and it is believed that Td3701 could be used for selective gene delivery.
Kuriyama
Shinichi
S
Pharmaceutical Research Center, Mochida Pharmaceutical Co., Ltd., Gotemba 412-8524, Japan.
Taguchi
Yasushi
Y
Nishimura
Kanako
K
Mizuguchi
Kiyoshi
K
Kobayashi
Kazutoshi
K
Katayama
Yoshiki
Y
Yanagibashi
Kazutoshi
K
Niidome
Takuro
T
D000595:Amino Acid Sequence; D000818:Animals; D002522:Chlorocebus aethiops; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005169:Factor VIII / Q000737:chemistry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000378:metabolism*; D008081:Liposomes; D008969:Molecular Sequence Data; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D014162:Transfection; D014709:Vero Cells
D008081:Liposomes; D010455:Peptides; D010718:Phosphatidylserines; D005169:Factor VIII; D004247:DNA