[Identification of biomarkers in the serum of the patients with poorly differentiated gastric cancer].
OBJECTIVE
To investigate the application of proteomics in the field of serology,and to screen the differential expression proteins related with poorly differentiated gastric carcinoma.


METHODS
Two-dimensional electrophoresis (2-DE) was applied to segregate the total proteins in the serum form gastric cancer patients and health volunteers. After staining,the differential expression proteins were analyzed using PDQuest software,and identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS).


RESULTS
Electrophoresis figures with high resolution and reproducibility were obtained. Six differential expression proteins were found only in the serum from gastric cancer patients, while four other proteins from healthy volunteers.


CONCLUSIONS
Protein expression is differential in the serum from the gastric cancer patients and health volunteers. It is hopeful to find the biomarkers related with poorly differentiated gastric carcinoma using proteomics.
Li
Wei
W
Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai Institute of Digestive Surgery, Shanghai 200025, China.
Liu
Bing-ya
BY
Li
Jian-fang
JF
Chen
Xue-hua
XH
Hao
Jian-hong
JH
Cai
Qu
Q
Gu
Qin-long
QL
Zhu
Zheng-gang
ZG
Lin
Yan-zhen
YZ
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D040901:Proteomics; D044967:Serum / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000097:blood* / Q000473:pathology*
D014408:Biomarkers, Tumor