Novel block copolymer (PPDO/PLLA-b-PEG): enhancement of DNA uptake and cell transfection.
The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co-L-lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the beta-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by beta-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy.
Bhattarai
Shanta Raj
SR
Department of Bionanosystem Engineering, Chonbuk National University, Chonju, South Korea.
Yi
Ho-Keun
HK
Bhattarai
Narayan
N
Hwang
Pyoung Han
PH
Kim
Hak Yong
HY
D016475:3T3 Cells; D000818:Animals; D001692:Biological Transport; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms; D004247:DNA / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008422:Materials Testing; D051379:Mice; D010714:Phosphatidylethanolamines; D010957:Plasmids / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D014162:Transfection / Q000379:methods*
D010714:Phosphatidylethanolamines; D011091:Polyesters; C483760:poly(p-dioxanone-co-L-lactide)-block-poly(ethylene glycol); D011092:Polyethylene Glycols; C020888:1,2-dielaidoylphosphatidylethanolamine; D004247:DNA