Up-regulation of chemokine ligand 20 in chronic rhinosinusitis.
OBJECTIVE
To investigate the up-regulation of chemokine ligand 20 (CCL20) in chronic rhinosinusitis mucosa and to localize the distribution of CCL20 in the human paranasal sinus mucosa.


METHODS
Prospective study.


METHODS
Tertiary academic institution.


METHODS
Ten patients who underwent functional endoscopic sinus surgery for chronic rhinosinusitis without nasal polyps and 10 normal control subjects.


METHODS
Messenger RNA was extracted from the sinus mucosa, and semiquantitative reverse transcriptase-polymerase chain reaction was performed. Immunohistochemical staining was used to localize the CCL20 protein.


RESULTS
The expression levels of CCL20 messenger RNA level in chronic rhinosinusitis without nasal polyps were significantly increased compared with those in normal sinus mucosa. The expression of CCL20 protein was greater in chronic rhinosinusitis without nasal polyps mucosa and was localized to the epithelial and submucosal glandular cells.


CONCLUSIONS
CCL20 is an inducible product of human paranasal sinus epithelium that may play a role in modulating mucosal immunity of the sinus mucosa.
Lee
Jong Hyouk
JH
Department of Otorhinolaryngology-Head and Neck Surgery, Hallym University College of Medicine, Seoul, South Korea.
Kang
Hee Joon
HJ
Woo
Jeong-Soo
JS
Chae
Sung Won
SW
Lee
Sang Hag
SH
Hwang
Soon Jae
SJ
Lee
Heung-Man
HM
D000328:Adult; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000235:genetics*; D002908:Chronic Disease; D004724:Endoscopy; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D018928:Immunity, Mucosal / Q000235:genetics; D019402:Macrophage Inflammatory Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000473:pathology; D010256:Paranasal Sinuses / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D012016:Reference Values; D012220:Rhinitis / Q000235:genetics* / Q000601:surgery; D012852:Sinusitis / Q000235:genetics* / Q000601:surgery; D015854:Up-Regulation / Q000235:genetics*
C108355:CCL20 protein, human; D054418:Chemokine CCL20; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger