title: Differences in the regulation of microtubule stability by the pro-rich region variants of microtubule-associated protein 4.
abstract: We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery after photobleaching analyses revealed that the interaction of MAP4-SP with the microtubules is very rapid, with a half-time of fluorescence recovery of 7 +/- 2.36 s, compared to 19.5 +/- 3.03 s in case of MAP4-LP. The dynamic interaction of MAP4-SP with microtubules in neural cells may contribute to the dynamic behaviors of extending neurites.
authors.affiliation: Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan.
authors.forename: Mohammad Rubayet
authors.initials: MR
authors.lastname: Hasan
authors.forename: Mingyue
authors.initials: M
authors.lastname: Jin
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Matsushima
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Susumu
authors.initials: S
authors.lastname: Kotani
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins