title: Probing protein function with small molecules.
abstract: The interface of chemistry and biology offers many opportunities to explore different aspects of cell biology. The emerging field of chemical genetics is providing the chemical means to understand biological systems not easily accessible using classical genetic manipulations. In this article, we will discuss how natural product mode of action studies and novel bio-organic manipulation of intracellular protein levels are proving useful in the exploration of cell biology.
authors.affiliation: Department of Molecular, Cell and Development Biology, New Haven, CT 06520-8103, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Gough
authors.forename: C M
authors.initials: CM
authors.lastname: Crews
mesh_terms: D000818:Animals; D019921:Biotinylation; D020650:Combinatorial Chemistry Techniques; D015195:Drug Design; D004353:Drug Evaluation, Preclinical; D023281:Genomics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007659:Ketones / Q000737:chemistry; D008956:Models, Chemical; D015336:Molecular Probe Techniques; D036103:Nanotechnology; D009842:Oligopeptides / Q000737:chemistry; D010766:Phosphorylation; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D011944:Receptors, Androgen / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012694:Serine / Q000031:analogs & derivatives / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry
chemical_list: D007659:Ketones; D009842:Oligopeptides; D011506:Proteins; D011944:Receptors, Androgen; D011993:Recombinant Fusion Proteins; D012717:Sesquiterpenes; C116212:dihydroeponemycin; D049452:Green Fluorescent Proteins; C002669:parthenolide; D012694:Serine; D044767:Ubiquitin-Protein Ligases; C078846:epoxomicin