title: Isolation of DNA from archival Papanicolaou stained cytological smears using a simple salting-out procedure.
abstract: DNA from archival Papanicolaou stained and unstained cytological smears was successfully isolated using a simple, rapid and inexpensive salting-out procedure. The quality of DNA was controlled by polymerase chain reaction (PCR) amplification of segments of the human beta-globin, human beta-actin and human papillomavirus L1 genes. Only negligible differences in amplification efficiency were observed between DNA isolated from stained and unstained smears. The salting-out procedure is a more rapid method for the isolation of DNA than phenol-chloroform extraction and may be used in instances where fresh or cryopreserved clinical specimens are not available.
authors.affiliation: Institute of Microbiology, Medical Faculty of Ljubljana, Zaloska 4, 61105 Ljubljana, Slovenia.
authors.forename: M
authors.initials: M
authors.lastname: Poljak
authors.forename: J
authors.initials: J
authors.lastname: Barlic
authors.forename: K
authors.initials: K
authors.lastname: Seme
authors.forename: T
authors.initials: T
authors.lastname: Avsic-Zupanc
authors.forename: A
authors.initials: A
authors.lastname: Zore