title: Monitoring of ketogenic diet for carnitine metabolites by subcutaneous microdialysis.
abstract: The ketogenic diet (KD) provides ketones from the degradation of free fatty acids for energy metabolism. It is a therapeutic option for pharmacoresistant epilepsies. Carnitine is the carrier molecule that transports fatty acids across the mitochondrial membrane for degradation into ketones. The integrity of this transport system is a prerequisite for an adequate ketogenic response. For monitoring of tissue metabolism with KD, we used the sampling method of s.c. microdialysis (MD), which permits minimally invasive, frequent, and extensive metabolic monitoring independent of blood tests. By using this new method, we monitored changes in carnitine metabolism induced by KD, particularly in free carnitine (C0), acetylcarnitine (C2), and hydroxybutyrylcarnitine (C4OH). Correlation of microdialysate and tissue concentrations for carnitines in vitro was about 85%. Carnitine metabolism was monitored in seven children started on a KD for pharmacoresistant epilepsy after a conventional initial fasting period. Detected metabolic changes consisted of a slight decrease in s.c. C0 and a marked increase in C2/CO and C4OH/CO levels. The levels of s.c. C4OH strongly correlate with beta-hydroxybutyrate (beta-OHB) levels in plasma providing an additional parameter for the carnitine reserve of the body and reflect an optimal ketogenic energy supply. Subcutaneous MD allows close and extensive monitoring of metabolism with a KD.
authors.affiliation: Department of Pediatric Neurology, Children's Hospital, Technical University Munich, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Hack
authors.forename: Verena
authors.initials: V
authors.lastname: Busch
authors.forename: Bettina
authors.initials: B
authors.lastname: Pascher
authors.forename: Raymonde
authors.initials: R
authors.lastname: Busch
authors.forename: Iris
authors.initials: I
authors.lastname: Bieger
authors.forename: Klaus
authors.initials: K
authors.lastname: Gempel
authors.forename: Friedrich A M
authors.initials: FA
authors.lastname: Baumeister
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000108:Acetylcarnitine / Q000032:analysis / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002331:Carnitine / Q000031:analogs & derivatives / Q000032:analysis* / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004032:Diet; D004827:Epilepsy / Q000178:diet therapy / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D007223:Infant; D007659:Ketones / Q000008:administration & dosage / Q000494:pharmacology*; D017551:Microdialysis / Q000379:methods*; D040521:Subcutaneous Tissue / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D007659:Ketones; C513202:hydroxybutyrylcarnitine; C427065:butyrylcarnitine; D000108:Acetylcarnitine; D002331:Carnitine; D020155:3-Hydroxybutyric Acid