Gene expression analysis of ELF-MF exposed human monocytes indicating the involvement of the alternative activation pathway.
This study focused on the cell activating capacity of extremely low frequency magnetic fields (ELF-MF) on human umbilical cord blood-derived monocytes. Our results confirm the previous findings of cell activating capacity of ELF-MF (1.0 mT) in human monocytes, which was detected as an increased ROS release. Furthermore, gene expression profiling (whole-genome cDNA array Human Unigene RZPD-2) was performed to achieve a comprehensive view of involved genes during the cell activation process after 45 min ELF-MF exposure. Our results indicate the alteration of 986 genes involved in metabolism, cellular physiological processes, signal transduction and immune response. Significant regulations could be analyzed for 5 genes (expression >2- or <0.5-fold): IL15RA (Interleukin 15 receptor, alpha chain), EPS15R (Epidermal growth factor receptor pathway substrate 15 - like 1), DNMT3A (Hypothetical protein MGC16121), DNMT3A (DNA (cytosine-5) methyltransferase 3 alpha), and one gene with no match to known genes, DKFZP586J1624. Real-time RT-PCR analysis of the kinetic of the expression of IL15RA, and IL10RA during 45 min ELF-MF exposure indicates the regulation of cell activation via the alternative pathway, whereas the delayed gene expression of FOS, IL2RA and the melatonin synthesizing enzyme HIOMT suggests the suppression of inflammatory processes. Accordingly, we suggest that ELF-MF activates human monocytes via the alternative pathway.
Lupke
Madeleine
M
Division of Environmental Physiology, Institute of Cell Biology and Biosystems Technology, University of Rostock, Albert-Einstein-Str. 3, D-18059 Rostock, Germany.
Frahm
Jana
J
Lantow
Margareta
M
Maercker
Christian
C
Remondini
Daniel
D
Bersani
Ferdinando
F
Simkó
Myrtill
M
D002478:Cells, Cultured; D000090205:DNA Methyltransferase 3A; D004574:Electromagnetic Fields; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007700:Kinetics; D009000:Monocytes / Q000378:metabolism*