title: Levels of tissue inhibitor of metalloproteinases 1 in plasma and urine from patients with bladder cancer.
abstract: OBJECTIVE
  To assess the potential use of plasma and urine levels of tissue inhibitor of metalloproteinases 1 (TIMP-1) in urothelial cancer.
  
  
  METHODS
  TIMP-1 levels were determined in urine and plasma from healthy donors (n=26), patients with bacterial bladder infection (n=24), urothelial bladder adenoma (n=3) or adenocarcinoma (n=7).
  
  
  RESULTS
  Free and total TIMP-1 in plasma were weakly but significantly correlated with age; urinary TIMP-1 was not. A strong correlation between free and total TIMP-1 in plasma was observed, with an average ratio of 0.85. No correlation between total TIMP-1 in urine and plasma was found (p=0.55). No significant differences in free or total TIMP-1 in plasma were found between healthy individuals, patients with cystitis or bladder cancer (p=0.4). Urinary TIMP-1 levels were significantly increased in patients with cystitis (p=0.001). No apparent differences in TIMP-1 levels were found in patients with bladder cancer at different stages.
  
  
  CONCLUSIONS
  Our previous observation of a weak but significant correlation between plasma TIMP-1 and age was confirmed. Likewise, an association between free and total TIMP-1 in plasma with a ratio of 0.85 was established. No correlation between plasma and urine TIMP-1 was found. Measurement of TIMP-1 in plasma and/or urine is apparently not useful for the identification of bladder cancer.
authors.affiliation: Department of Veterinary Pathology, The Veterinary University, Frederiksberg. mholten@dadlnet.dk
authors.forename: M N
authors.initials: MN
authors.lastname: Holten-Andersen
authors.forename: N
authors.initials: N
authors.lastname: Brünner
authors.forename: H J
authors.initials: HJ
authors.lastname: Nielsen
authors.forename: I J
authors.initials: IJ
authors.lastname: Christensen
authors.forename: N Møller
authors.initials: NM
authors.lastname: Sørensen
authors.forename: A S Schrohl
authors.initials: AS
authors.lastname: Rasmussen
authors.forename: H
authors.initials: H
authors.lastname: Primdahl
authors.forename: T
authors.initials: T
authors.lastname: Orntoft
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis; D000236:Adenoma / Q000175:diagnosis; D000368:Aged; D000369:Aged, 80 and over; D001424:Bacterial Infections / Q000175:diagnosis; D003404:Creatinine / Q000652:urine; D003556:Cystitis / Q000097:blood / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood* / Q000652:urine*; D001745:Urinary Bladder Diseases / Q000175:diagnosis; D001749:Urinary Bladder Neoplasms / Q000097:blood* / Q000652:urine*
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D003404:Creatinine