Analysis of paraphenylenediamine.
The toxic Paraphenylenediamine is characterized by infrared spectrophotometry and nuclear magnetic resonance spectroscopy, whereas, high performance liquid chromatography with a refractive index detector was used to determine its purity in the suspect samples using external standardisation. An analytical method for determination of lower traces of paraphenylenediamine in post-mortem biological fluids was developed. This procedure involves deproteneization or hydrolysis followed by liquid-liquid extraction and derivatization with trifluoroacetic anhydride. 1 microL of the extract was then analysed by gas chromatography/iontrap mass spectrometry. Benzidine used as the internal standard for quantification and the extraction recovery test was evaluated to 85%. This method was validated in cases with paraphenylenediamine poisoning.
Bellimam
M A
MA
Laboratoire de Recherches et d'Analyses et Scientifiques de la Gendarmerie Royale, BP. 6597, Rabat-lnstituts, CP 10100 Rabat-Maroc.
Stambouli
A
A
El Karni
N
N
Bouayoun
T
T
El Bouri
A
A
D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D010655:Phenylenediamines / Q000097:blood* / Q000506:poisoning / Q000652:urine*
D010655:Phenylenediamines; C029728:4-phenylenediamine