Protective effect of nitric oxide on iron deficiency-induced oxidative stress in maize (Zea mays).
The effects of nitric oxide (NO) in protecting maize (Zea mays) leaves against iron deficiency-induced oxidative stress were investigated. The increased contents of hydrogen peroxide (H(2)O(2)) and superoxide (O(2)(-)*) due to iron deficiency suggested oxidative stress. The increased contents of thiobarbituric acid-reacting substances (TBARS) and the decreased contents of protein-bound thiol (PT) and non-protein-bound thiol (NPT) indicated iron deficiency-induced oxidative damage on proteins and lipids. Sodium nitroprusside (SNP), a nitric oxide (NO) donor, partially reversed iron deficiency-induced retardation of plant growth as well as chlorosis. Reduced contents of H(2)O(2), O(2)(-)*, TBARS and increased contents of PT and NPT also indicated that NO alleviated iron deficiency-induced oxidative damage. The activities of SOD and GR decreased sharply while the activities of CAT, POD and APX increased under SNP treatment. Our data suggest that NO can protect maize plants from iron deficiency-induced oxidative stress by reacting with ROS directly or by changing activities of ROS-scavenging enzymes.
Sun
Baoteng
B
Key Laboratory of Arid and Grassland Ecology, School of Life Science, Lanzhou University, Lanzhou 730000, China.
Jing
Yan
Y
Chen
Kunming
K
Song
Lili
L
Chen
Fangjian
F
Zhang
Lixin
L
D000975:Antioxidants / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D000090463:Iron Deficiencies; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress; D018515:Plant Leaves / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*
D000975:Antioxidants; D009569:Nitric Oxide; D007501:Iron