A rapid and simple method for DNA extraction from yeasts and fungi isolated from Agave fourcroydes.
A simple and easy protocol for extracting high-quality DNA from different yeast and filamentous fungal species is described. This method involves two important steps: first, the disruption of cell walls by mechanical means and freezing; and second, the extraction, isolation, and precipitation of genomic DNA. The absorbance ratios (A(260)/A(280)) obtained ranged from 1.6 to 2.0. The main objective of this procedure is to extract pure DNA from yeast and filamentous fungi, including those with high contents of proteins, polysaccharides, and other complex compounds in their cell walls. The yield and quality of the DNAs obtained were suitable for micro/minisatellite primer-polymerase chain reaction (MSP-PCR) fingerprinting as well as for the sequence of the D1/D2 domain of the 26S rDNA.
Tapia-Tussell
Raul
R
Laboratorio GeMBio, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida 97200, Yucatán, México.
Lappe
Patricia
P
Ulloa
Miguel
M
Quijano-Ramayo
Andrés
A
Cáceres-Farfán
Mirbella
M
Larqué-Saavedra
Alfonso
A
Perez-Brito
Daisy
D
D027882:Agave / Q000382:microbiology*; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005658:Fungi / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013997:Time Factors
D004271:DNA, Fungal