Molecular profiling and comparison of field transmissible spongiform encephalopathy cases diagnosed in Catalunya.
Molecular profiling of the proteinase K resistant prion protein (PrP(res)) is a technique that has been applied to the characterisation of transmissible spongiform encephalopathy (TSE) strains. An interesting example of the application of this technique is the ability to differentiate, at the experimental level, between bovine spongiform encephalopathy (BSE) and scrapie infection in sheep, and to distinguish between classical and atypical BSE and scrapie cases. Twenty-six BSE cases and two scrapie cases from an active TSE surveillance program and diagnosed at the PRIOCAT, TSE Reference Laboratory (Centre de Recerca en Sanitat Animal, Universitat Autònoma de Barcelona, Catalunya, Spain) were examined by Western blotting. Molecular profiling was achieved by comparing the glycosylation profile, deglycosylated PrP molecular weight and 6H4/P4 monoclonal antibody binding ratio. The results obtained during the characterisation of these field cases indicated an absence of atypical BSE cases in Catalunya.
Vidal
E
E
Laboratori PRIOCAT, Centre de Recerca en Sanitat Animal, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain.
Márquez
M
M
Costa
C
C
Tortosa
R
R
Domènech
A
A
Serafín
A
A
Pumarola
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D019286:Endopeptidase K / Q000378:metabolism*; D006031:Glycosylation; D015151:Immunoblotting / Q000662:veterinary; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018620:PrPSc Proteins / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D012608:Scrapie / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D012756:Sheep; D013030:Spain
D018620:PrPSc Proteins; D019286:Endopeptidase K