title: Human rotavirus serotype G9, São Paulo, Brazil, 1996-2003.
abstract: A total of 3,101 fecal specimens were collected during an 8-year survey for rotavirus infection in São Paulo, Brazil. Group A rotavirus was detected in 774 (25.0%) specimens. Of these, 431 strains (55.7%) were analyzed for G and P types by reverse transcription-polymerase chain reaction; G1 was the predominant serotype (68.2%), followed by G9 (17.2%), G4 (6.3%), G2 (1.2%), G3 (0.7%), mixed infection (1.8%), and untypeable (4.6%). Both rotavirus G and P types could be established in 332 strains (77.0%). We identified the 4 most common strains worldwide: P[8]G1 (66.6%), P[4]G2 (1.0%), P[8]G3 (0.6%), and P[8]G4 (7.2%). Among the single G9 strains detected, VP4 genotyping showed that P[8]G9 was the most prevalent, followed by P[4]G9 and P[6]G9. The emergence and high frequency of rotavirus G9 in São Paulo, Brazil, and other parts of the world will affect the development and evaluation of future vaccines.
authors.affiliation: Laboratório de Vírus Entéricos, Adolfo Lutz Institute, São Paulo, Brazil. rcarmona@ial.sp.gov.br
authors.forename: Rita Cássia Compagnoli
authors.initials: RC
authors.lastname: Carmona
authors.forename: Maria do Carmo Sampaio Tavares
authors.initials: Mdo C
authors.lastname: Timenetsky
authors.forename: Simone Guadagnucci
authors.initials: SG
authors.lastname: Morillo
authors.forename: Leonardo José
authors.initials: LJ
authors.lastname: Richtzenhain
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D006801:Humans; D008875:Middle Aged; D015995:Prevalence; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D012703:Serotyping
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral