Identification of conjugated linoleic acid elongation and beta-oxidation products by coupled silver-ion HPLC APPI-MS.
Atmospheric pressure photoionisation (APPI) was used in combination with silver-ion (Ag(+))-HPLC for detection of (conjugated) fatty acid methyl esters (FAME) by tandem-mass spectrometry. APPI-MS of methyl esters of conjugated linoleic acid showed an increase in signal-to-noise ratio by a factor of 40 compared to atmospheric pressure chemical ionization in the positive mode. It was possible to identify double bond position, configuration and chain length of FAME based on chromatographic separation and mass detection. The developed LC-MS method is useful for the analysis of CLA elongation and beta-oxidation products, especially with trans,trans-configuration, which are difficult to analyze by conventional GC-MS techniques.
Müller
André
A
Institute of Biochemistry and Food Chemistry, Department of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Mickel
Markus
M
Geyer
Roland
R
Ringseis
Robert
R
Eder
Klaus
K
Steinhart
Hans
H
D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019787:Linoleic Acid / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*
D012834:Silver; D019787:Linoleic Acid