Effect of H-7 and Lat-B on retinal physiology.
OBJECTIVE
To investigate the effects of H-7 and Latrunculin B (Lat-B) on retinal vascular permeability and electrophysiology at concentrations that increase outflow facility in monkeys.


METHODS
One eye of 1 rhesus and 22 cynomolgus monkeys received an intravitreal bolus injection of H-7 or Lat-B; the opposite eye received vehicle. Multifocal electroretinograms (mfERGs), and photopic and scotopic full-field electroretinograms (ffERGs, sERGs) were recorded in subsets of monkeys at baseline and at multiple time-points post-H-7 or Lat-B. Vitreous fluorophotometry (VF) and fluorescein angiography (FA) were also performed.


RESULTS
No differences between the H-7 or Lat-B treated and control eyes were found in ffERGs, mfERGs, sERGs, or in FAs in any monkey. No significant difference was found in vitreous fluorescein levels between H-7 treated or Lat-B treated vs. control eyes.


CONCLUSIONS
No effect on retinal vascular permeability or retinal electrophysiology was apparent after intravitreal administration of H-7 or Lat-B at doses that increase outflow facility and lower IOP when given intracamerally.
Kiland
J A
JA
Department of Ophthalmology & Visual Sciences, University of Wisconsin Medical School, Madison, WI 53792, USA. jkiland@wisc.edu
Miller
C L
CL
Kim
C B Y
CB
Ver Hoeve
J N
JN
Gabelt
B T
BT
Peterson
J
J
Nork
T M
TM
Kaufman
P L
PL
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D003623:Dark Adaptation; D004596:Electroretinography / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005451:Fluorescein Angiography; D015590:Fluorophotometry; D007267:Injections; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008387:Marine Toxins / Q000494:pharmacology; D010775:Photic Stimulation; D012160:Retina / Q000187:drug effects* / Q000502:physiology; D012171:Retinal Vessels / Q000502:physiology; D013844:Thiazoles / Q000494:pharmacology*; D053778:Thiazolidines; D014822:Vitreous Body / Q000187:drug effects / Q000378:metabolism
D019086:Bridged Bicyclo Compounds, Heterocyclic; D004791:Enzyme Inhibitors; D008387:Marine Toxins; D013844:Thiazoles; D053778:Thiazolidines; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C037068:latrunculin B