Transfection of oral squamous cell carcinoma with human papillomavirus-16 induces proliferative and morphological changes in vitro.
BACKGROUND
Human papillomavirus has been implicated in virtually all cervical cancers and is believed to be the primary etiological factor that transforms cervical epithelia. The presence of HPV in oral cancers suggests that HPV may play a similar role in transforming the oral epithelia. The prevalence of HPV in oral cancers is highly variable, however, presenting problematic issues regarding the etiology of oral cancers, which must be investigated more thoroughly. Past analyses of HPV in cancers of the oral cavity have largely been confined to retrospective studies of cancer patients. The purpose of this study was to examine the potential for HPV16 infection to alter the proliferative phenotype of oral squamous cell carcinoma in vitro.


RESULTS
This study found that the oral squamous cell carcinoma cell line, CAL27, transfected with HPV16, exhibited significantly increased proliferation, compared with non-transfected CAL27. The increased proliferation was observed under low density conditions, even in the absence of serum. Moreover, these effects were specific to proliferation, adhesion, and morphology, while cell viability was not affected.


CONCLUSIONS
This study represents one of the first investigations of the effects of HPV16 infection on the proliferation, adhesion, and morphology of an oral squamous cell carcinoma cell line in vitro. The finding that HPV16 has the ability to measurably alter adhesion and proliferative potential is significant, indicating that HPV may have multiple influences on precancerous and cancerous lesions and should be explored as a risk factor and mediator of cancer phenotypes. These measurements and observations will be of benefit to researchers interested in elucidating the mechanisms of oral cancer transformation and the factors governing carcinogenesis and progression.
Kingsley
Karl
K
Department of Biomedical Sciences, University of Nevada, Las Vegas - School of Dental Medicine, 1001 Shadow Lane B315, Las Vegas, Nevada, 89106, USA.
Johnson
Devin
D
Department of Biomedical Sciences, University of Nevada, Las Vegas - School of Dental Medicine, 1001 Shadow Lane B315, Las Vegas, Nevada, 89106, USA.
O'Malley
Susan
S
Department of Biomedical Sciences, University of Nevada, Las Vegas - School of Dental Medicine, 1001 Shadow Lane B315, Las Vegas, Nevada, 89106, USA.