title: Mesenchymal stem cells isolated from the human bone marrow: cultivation, phenotypic analysis and changes in proliferation kinetics.
abstract: Mesenchymal Stem Cells (MSCs) are rare elements living in various organs (e.g., bone marrow), able to differentiate into specialized tissues, such as bone, cartilage, tendon, and myocardium. Since the first description of MSCs by Fridenshtein, several investigators have shown that these cells can also differentiate into chondrocytes, adipocytes, and, at least, in rodents into skeletal myoblasts. Later on, more primitive progenitor cells were characterized, able to give rise not only to limb-bud mesoderm, but also to cells of visceral mesoderm. Those cells were named mesodermal progenitor cells (MPCs). The aim of our study was to characterize and compare the biological properties and spontaneous differentiation potential of two different cell types (MSCs and MPCs) isolated from the human vertebral body bone marrow. The results of our experiments proved that the MPCs can be expanded beyond Hayflick's limit and differed from MSCs in morphology, biological and phenotypic characteristics. Because of their high proliferative and differentiation potential, MPCs can become more attractive source of adult stem cells for therapeutic purposes.
authors.affiliation: Department of Histology and Embryology, Charles University in Prague, Faculty of Medicine, Hradec Králové, Czech Republic. soukupto@lfhk.cuni.cz
authors.forename: Tomás
authors.initials: T
authors.lastname: Soukup
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Mokrý
authors.forename: Jana
authors.initials: J
authors.lastname: Karbanová
authors.forename: Robert
authors.initials: R
authors.lastname: Pytlík
authors.forename: Petr
authors.initials: P
authors.lastname: Suchomel
authors.forename: Lenka
authors.initials: L
authors.lastname: Kucerová
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D008648:Mesoderm / Q000166:cytology / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology