title: Stable isotope metabolic labeling of Neisseria meningitidis lipooligosaccharide.
abstract: The lipooligosaccharide (LOS) of a Neisseria meningitidis acetate auxotroph was metabolically labeled with either [2-13C]-sodium acetate or [1,2-13C2]-sodium acetate. In this study, we demonstrated that this label was efficiently incorporated into both the lipid A acyl moieties and the two N-acetylglucosamines present in the oligosaccharide branch of the LOS. The development of this efficient labeling protocol should prove useful in future structural studies analyzing the interactions between LOS and host proteins.
authors.affiliation: The Buck Institute for Age Research, Novato, California 94945, USA.
authors.forename: Deborah M B
authors.initials: DM
authors.lastname: Post
authors.forename: Desheng
authors.initials: D
authors.lastname: Zhang
authors.forename: Jerrold P
authors.initials: JP
authors.lastname: Weiss
authors.forename: Bradford W
authors.initials: BW
authors.lastname: Gibson
mesh_terms: D000085:Acetates / Q000737:chemistry; D002250:Carbon Radioisotopes; D004731:Endotoxins / Q000737:chemistry*; D007553:Isotope Labeling; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D013058:Mass Spectrometry; D008970:Molecular Weight; D009345:Neisseria meningitidis / Q000737:chemistry* / Q000254:growth & development; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes; D004731:Endotoxins; D008050:Lipid A; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides