Proteomic dissection of potential signal recognition particle dependence in protein secretion by Bacillus subtilis.
The bacterial signal recognition particle (SRP)-dependent pathway is believed to be a major targeting route for membrane proteins, as well as for subsets of secretory proteins. The present studies were aimed at an assessment of the role of two key components of SRP, namely Ffh and FtsY, in protein secretion by the Gram-positive bacterium Bacillus subtilis. Our results show that both components are important for the extracellular accumulation of proteins containing known signal peptides. Remarkably, extracellular accumulation of individual proteins was affected to different extents by depletion of Ffh or FtsY, at least under the conditions tested. Moreover, the observed Ffh or FtsY dependence of certain secretory proteins did not seem to correlate with signal peptide length or hydrophobicity. Although it is presently difficult to distinguish between direct and indirect effects, these findings suggest that other, yet unidentified, determinants in secretory proteins are also important for their SRP dependence. High-level production of homologous and heterologous secretory proteins was shown to result in elevated cellular Ffh and FtsY levels. This phenomenon is, most likely, due to post-transcriptional regulation. In conclusion, the present proteomic dissection of SRP-dependent extracellular protein accumulation provides exciting leads to identify novel determinants for interactions between secretory proteins and SRP.
Zanen
Geeske
G
Department of Pharmaceutical Biology, University of Groningen, The Netherlands.
Antelmann
Haike
H
Meima
Rob
R
Jongbloed
Jan D H
JD
Kolkman
Marc
M
Hecker
Michael
M
van Dijl
Jan Maarten
JM
Quax
Wim J
WJ
D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D018271:Signal Recognition Particle / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D010446:Peptide Fragments; D020543:Proteome; D018271:Signal Recognition Particle