title: Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite.
abstract: Nano-crystalline Zn-containing hydroxyapatite (ZnHAp) was prepared by the wet-chemical method and the selective adsorption of essential proteins was examined, taking bovine serum albumin (BSA) and pathogenic protein such as beta(2)-microglobulin (beta(2)-MG) as model proteins. Transmission electron microscopy observation and X-ray diffraction analysis indicated that the increase of Zn content led to smaller crystallites and their specific surface area of ZnHAps increased with increasing Zn content, accordingly. Furthermore, the amounts of BSA adsorption on ZnHAp particles decreased with increasing Zn content in spite of the increase in the specific surface area. It is thus concluded that nano-crystalline ZnHAps had a highly selective adsorption property with regard to beta(2)-MG.
authors.affiliation: Industrial Technology Center of Okayama Prefecture, 5301, Haga, Okayama-shi 701-1296, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Fujii
authors.forename: Mizuki
authors.initials: M
authors.lastname: Ohkubo
authors.forename: Kanji
authors.initials: K
authors.lastname: Tsuru
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hayakawa
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Osaka
authors.forename: Koji
authors.initials: K
authors.lastname: Kawabata
authors.forename: Christian
authors.initials: C
authors.lastname: Bonhomme
authors.forename: Florence
authors.initials: F
authors.lastname: Babonneau
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D003460:Crystallization; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D066298:In Vitro Techniques; D008422:Materials Testing; D008854:Microscopy, Electron; D008954:Models, Biological; D049329:Nanostructures; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties; D014961:X-Ray Diffraction; D015032:Zinc / Q000737:chemistry; D001613:beta 2-Microglobulin / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; D011506:Proteins; D001613:beta 2-Microglobulin; D012710:Serum Albumin, Bovine; D015032:Zinc