Establishment of the model of white blood cell membrane chromatography and screening of antagonizing TLR4 receptor component from Atractylodes macrocephala Koidz.
A model of white blood cell membrane chromatography (WB-CMC) was established to screen active component from Atractylodes macrocephala Koidz. The component can antagonize Toll-like receptor 4 (TLR4) and inhibit inflammatory reaction. In the model of WB-CMC, cell membrane stationary phase (CMSP) was prepared by immobilizing the rabbit white blood cell membrane (WBCM) onto the surface of silica carrier and taxinol was used as a model molecule. The active component which can act on WBCM and its receptor (such as TLR4) as an effective target in A. macrocephala was determined by using a replacement experiment. The anti-inflammatory effects of the active component were tested by using pharmacological methods in vivo. The results indicated that the retention characteristics of atractylenolide I as active component was similar to that of taxinol in the model of WB-CMC. And so, atractylenolide I acted on the WBCM and TLR4 and its anti-inflammatory activity was related with antagonizing TLR4. Therefore, the interaction between the active component and WBCM and its receptor can be simulated by the model of WB-CMC in vitro. This model can be used to screen active components and to study effective characteristics for acting on definite targets.
Li
Cuiqin
C
School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
He
Langchong
L
D019342:Acetic Acid; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D031174:Atractylodes / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry* / Q000648:ultrastructure; D002845:Chromatography / Q000379:methods*; D004427:Ear Diseases / Q000139:chemically induced / Q000517:prevention & control; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D005260:Female; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007962:Leukocytes / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010538:Peritonitis / Q000139:chemically induced / Q000517:prevention & control; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011817:Rabbits; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors / Q000737:chemistry; D014992:Xylenes
D000893:Anti-Inflammatory Agents; D007783:Lactones; D010936:Plant Extracts; D012717:Sesquiterpenes; D051197:Toll-Like Receptor 4; D014992:Xylenes; C424804:atractylenolide I; D019342:Acetic Acid