In vitro effects of recombinant zebrafish IFN on spring viremia of carp virus and infectious hematopoietic necrosis virus.
In order to evaluate the effects of fish recombinant interferon (rIFN) on fish pathogenic rhabdoviruses, the zebrafish (Danio rerio) IFN (DreIFN) allele B gene was cloned and expressed in Escherichia coli. In addition, the effects of recombinant DreIFN (rDreIFN) on spring viremia of carp virus (SVCV), infectious hematopoietic necrosis virus (IHNV), and vesicular stomatitis virus (VSV) were surveyed in fish and chicken cells. The mature peptide of DreIFN allele B gene encodes 163 amino acids. Residues 3 and 98 are a pair of cysteines that likely form an intrachain disulfide bridge. rDreIFN protein was detected as a band at 21.6 kDa by SDS-PAGE. The purified rDreIFN has anti-SVCV and anti-IHNV activity of 3 x 10(4) U/mg-10(7) U/mg. The results indicate that rDreIFN has higher activity against SVCV and IHNV on epithelioma papulosum cyprinid (EPC) than on grass carp (C. idellus) ovary (CO) cell lines and no activity against VSV on chick embryo fibroblasts (CEF).
Wang
Li
L
Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.
Wang
Lei
L
Zhang
He-Xiao
HX
Zhang
Jian-Hua
JH
Chen
Wei-Hong
WH
Ruan
Xiao-Fei
XF
Xia
Chun
C
D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029142:Infectious hematopoietic necrosis virus / Q000187:drug effects*; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D018116:Vesiculovirus / Q000187:drug effects*; D029961:Zebrafish Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*
D007370:Interferon Type I; D011994:Recombinant Proteins; D029961:Zebrafish Proteins