[The influence of nitric oxide inductor (NaNO2) on the sensitivity of Chinese hamster cells to hyperthermia].
Formerly we reported the reduction of sensitivity to gamma- and UV-radiation in Chinese hamster cells of line 90 under the influence of nitric oxide inductor (NaNO2). Of interest was to learn if it possible to reduce the influence of hyperthermia on cells by means of NO inductor. A 1 h long incubation with this NO donor demonstrated an increased survival of cells heated up to 45 degrees C, and a decreased frequency of chromosomal aberrations in these. Employment of cycloheximide (CHE), an inhibitor of protein synthesis, and administration of CHE together with nitric oxide donor (NaNO2), equally increased the cell survival at hyperthermia. These and relevant literature data suggest that the demonstrated effect of exogenous NO may be associated with HSP70 protein. The noticed decrease in the number of chromosomal aberrations in heated cells under the influence of NO donor may play an important role in its modifying effect on cells at hyperthermia.
Bol'shakova
O I
OI
Sverdlov
A G
AG
Timoshenko
S I
SI
Nikanorova
N G
NG
Grachev
S A
SA
D000818:Animals; D002460:Cell Line / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003513:Cycloheximide / Q000494:pharmacology; D004357:Drug Synergism; D006361:Heating; D006358:Hot Temperature; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology
D020030:Nitric Oxide Donors; D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide