[Quantitative study of osteoclastic related factors in the process of bone reconstruction].
OBJECTIVE
To detect the expression of osteoclast related factors, tumor necrosis factor-alpha (TNF-alpha), receptor activator of NF-kappaB ligand (RANKL) and tartrate-resistant acid phosphatase (TRAP), in the process of bone remodeling.


METHODS
8-week-old male C57BL/6J mice were employed in this study to detect the expression of osteoclast related factors by real time PCR.


RESULTS
TNF-alpha, RANKL and TRAP were up regulated in the process of bone remodeling, they reached the peak on day 2, 5 and 10 individually after injury.


CONCLUSIONS
Osteoclast related factors also participate in bone remodeling, which depends on the delicate balance between bone formation and bone resorption.
Chang
Xin
X
Dept. of Oral Science, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.
Hou
Zhi-Ming
ZM
Sibata
Yasuaki
Y
Tsukazaki
Tomoo
T
Yamaguchi
Akira
A
D000818:Animals; D001862:Bone Resorption; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B; D010010:Osteoclasts; D010012:Osteogenesis; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D014409:Tumor Necrosis Factor-alpha
D016328:NF-kappa B; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D014409:Tumor Necrosis Factor-alpha