title: [Mechanical properties of rat HCC adhesion to collagen I and its relationship with cell cycle].
abstract: The mechanical properties of tumor cells adhering to extracellular matrix (ECM) are closely related with their invasion and metastesis. In this study we investigated the adhesive mechanical properties between hepatocellular carcinoma cells(HCC) and the collagen I coated surfaces from the viewpoint of cell cycle by coupling cellular biology and cellular mechanics, using micropipette aspiration and cell synchronization technique. The results showed that the synchronous G1 and S phase HCC cells were achieved by use of thymine-2-desoryriboside, colchicines sequential blockage method and double thymine-2-desoryriboside blockage method, and that the synchronous rates of G1 and S phase HCC amounted to 74.09% and 90.39% respectively. Within the ranges of dosing and timing in this study, the adhesion of HCC cells to collagen I displayed dose dependent and time dependent patterns. S phase cells had small force of adhesion to collagen I as compared with G1 phase and controlled cells(P<0.001), which suggested that G1 phase HCC may play an important role in the step of invading interstitial connective tissue in the metastasis pathway of HCC through blood circulation. These are of significance to unveiling the mechanism of HCC metastasis.
authors.affiliation: Key Laboratory of Biomechanics and Tissue Engineering of the State Ministry of Education, College of Bioengineering. Chongqing University, Chongqing 400044, China. song9973@tom.com
authors.forename: Guanbi
authors.initials: G
authors.lastname: Song
authors.forename: Jian
authors.initials: J
authors.lastname: Qin
authors.forename: Runbin
authors.initials: R
authors.lastname: Yan
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Shen
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
authors.forename: Shaoxi
authors.initials: S
authors.lastname: Cai
authors.forename: Caixin
authors.initials: C
authors.lastname: Sun
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002453:Cell Cycle; D024042:Collagen Type I / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D009362:Neoplasm Metastasis; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D024042:Collagen Type I