[Effects of vasoactive intestinal peptide on LPS-induced MMP-9 expression by alveolar macrophages in rats].
OBJECTIVE
To explore the role of vasoactive intestinal peptide (VIP) on LPS-induced MMP-9 expression by alveolar macrophages (AM) in rats.


METHODS
LPS-induced cultured Wistar rats AMs were treated with different concentrations of VIP (10(-10) to approximately 10(-6) mol/L) for 24 h. AMs and the supernatant were collected to measure the MMP-9 expression and activity by RT-PCR and gelatin zymography, respectively. Results The MMP-9 activity and expression of LPS-induced AMs were significantly higher than those in the control group (P < 0.01). VIP (10(-9) to approximately 10(-6) mol/L) down-regulated LPS-induced MMP-9 activity and its expression. The effects were diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (P < 0.01).


CONCLUSIONS
VIP can decrease LPS-induced MMP-9 activity and its expression, which may be related to protein kinase C and calmodulin pathway. VIP may have protective roles in the lung injury.
Liu
Yong-ping
YP
Department of Physiology, Xiangya Medical College, Central South University, Changsha 410078, China.
Guan
Cha-xiang
CX
Bai
Hong-bo
HB
Qin
Xiao-qun
XQ
Liu
Hui-jun
HJ
D000818:Animals; D002147:Calmodulin / Q000378:metabolism; D002478:Cells, Cultured; D015536:Down-Regulation; D005260:Female; D008070:Lipopolysaccharides; D016676:Macrophages, Alveolar / Q000166:cytology / Q000378:metabolism*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D002147:Calmodulin; D008070:Lipopolysaccharides; D014660:Vasoactive Intestinal Peptide; D011493:Protein Kinase C; D020780:Matrix Metalloproteinase 9