New approaches to fluorescence in situ hybridization.
Fluorescence in situ hybridization (FISH) is a nonisotopic labeling and detection method that provides a direct way to determine the relative location or copy number of specific DNA sequences in nuclei or chromosomes. With recent advancements, this technique has found increased application in a number of research areas, including cytogenetics, prenatal diagnosis, cancer research and diagnosis, nuclear organization, gene loss and/or amplification, and gene mapping. The availability of different types of probe and the increasing number of FISH techniques has made it a widespread and diversely applied technology. Multicolor karyotyping by multicolor FISH and spectral karyotyping interphase FISH and comparative genomic hybridization allow genetic analysis of previously intractable targets. We present a brief overview of FISH technology and describe in detail methods of probe labeling and detection for different types of tissue sample, including microdissected nuclei from formalin-fixed paraffin-embedded tissue sections.
Murthy
Sabita K
SK
Medical Genetics, Al Wasl Hospital, Dubai, United Arab Emirates.
Demetrick
Douglas J
DJ
D002453:Cell Cycle; D002875:Chromosomes; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods*; D007621:Karyotyping / Q000379:methods; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D011296:Prenatal Diagnosis / Q000379:methods; D016615:Telomere / Q000378:metabolism
D005456:Fluorescent Dyes