title: Application of a column selection system and DryLab software for high-performance liquid chromatography method development.
abstract: This paper describes a strategy for the development of chromatographic methods for drug candidates based upon the use of simple MS compatible mobile phases and optimization of the chromatographic selectivity through variations of the stationary phase and mobile phase pH. The strategy employs an automated column selection system and a series of HPLC columns, varying in hydrophobicity and silanol activity, in combination with DryLab software to develop chromatographic methods for the separation of mixtures of bupivacaine and its metabolites; acidic, basic, and neutral compounds; and atenolol, nitrendipine, and their degradation products.
authors.affiliation: Department of Chemistry, University of Kansas, Mallott Hall, Lawrence, KS 66045, USA.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Krisko
authors.forename: Kieran
authors.initials: K
authors.lastname: McLaughlin
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Koenigbauer
authors.forename: Craig E
authors.initials: CE
authors.lastname: Lunte
mesh_terms: D001262:Atenolol / Q000032:analysis / Q000737:chemistry; D002045:Bupivacaine / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003198:Computer Simulation; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012984:Software; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013237:Stereoisomerism
chemical_list: D004364:Pharmaceutical Preparations; D001262:Atenolol; D002045:Bupivacaine