An essential role of Cdc42-like GTPases in mitosis of HeLa cells.
Here we used RNA interference and examined possible redundancy amongst Rho GTPases in their mitotic role. Chromosome misalignment is induced significantly in HeLa cells by Cdc42 depletion and not by depletion of either one or all of the other four Cdc42-like GTPases (TC10, TCL, Wrch1 or Wrch2), four Rac-like GTPases or three Rho-like GTPases. Notably, combined depletion of Cdc42 and all of the other four Cdc42-like GTPases significantly enhances chromosomal misalignment. These observations suggest that Cdc42 is the primary GTPase functioning during mitosis but that the other four Cdc42-like GTPases can also assume the mitotic role in its absence.
Yasuda
Shingo
S
Horizontal Medical Research Organization, Kyoto University, Faculty of Medicine, Japan.
Taniguchi
Hiroyuki
H
Oceguera-Yanez
Fabian
F
Ando
Yoshikazu
Y
Watanabe
Sadanori
S
Monypenny
James
J
Narumiya
Shuh
S
D001483:Base Sequence; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D034622:RNA Interference; D020764:cdc42 GTP-Binding Protein / Q000502:physiology*
D017931:DNA Primers; D020764:cdc42 GTP-Binding Protein