title: Insulin vesicle release: walk, kiss, pause ... then run.
abstract: The mechanisms by which insulin-containing dense core secretory vesicles approach and finally fuse with the plasma membrane are of considerable current interest: defects in these processes may be one of the contributing factors to Type 2 diabetes. In this review, we discuss the molecular mechanisms involved in vesicle trafficking within the pancreatic beta-cell and the mechanisms whereby these may be regulated. We then go on to describe recent evidence that suggests that vesicle fusion at the plasma membrane is a partly reversible process ("kiss and run" or "cavity recapture"). We propose that vesicles may participate in a exo-endocytotic cycle in which a proportion of those that have already undergone an interaction with the plasma membrane may exchange exocytotic machinery with maturing vesicles.
authors.affiliation: Department of Biochemistry, School of Medical Sciences, University Walk University of Bristol, Bristol, United Kingdom. g.a.rutter@bris.ac.uk
authors.forename: Guy A
authors.initials: GA
authors.lastname: Rutter
authors.forename: Elaine V
authors.initials: EV
authors.lastname: Hill
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000503:physiopathology*; D004705:Endocytosis / Q000502:physiology; D005089:Exocytosis / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D022142:Secretory Vesicles / Q000378:metabolism*
chemical_list: D007328:Insulin