Determination of lycopene and beta-carotene by high-performance liquid chromatography using sudan I as internal standard.
A new method, using sudan I as internal standard to determine the content of lycopene or beta-carotene in samples, was developed. According to UV-vis absorption spectra, sudan I, lycopene and beta-carotene all had absorption peaks at 450 nm. They could be separated absolutely by high-performance liquid chromatography (HPLC) with retention time of 2.7, 6.6 and 10.1 min, respectively. The related equations between sudan I and lycopene or beta-carotene content were obtained and verified by determining the content of lycopene or beta-carotene in Blakeslea trispora cells. The relative error was below 1.4% for determining lycopene content and below 1.9% for beta-carotene. Intra-day variability for lycopene determination was less than 3.4% and for beta-carotene was less than 1.4%. The mean recovery of lycopene or beta-carotene was 96.1 and 97.9%, respectively.
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College of Life Science and Technology, Beijing University of Chemical Technology, P.O. Box 75, Beijing 100029, PR China.
Yuan
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Dong
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D000465:Algorithms; D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077276:Lycopene; D009284:Naphthols / Q000032:analysis* / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D019207:beta Carotene / Q000032:analysis*
D009284:Naphthols; D019207:beta Carotene; D002338:Carotenoids; C024336:1-phenylazo-2-naphthol; D000077276:Lycopene