Correlation between pharmacological efficacy of cyclosporine A and tacrolimus, evaluated by lymphocyte immunosuppressant-sensitivity test (LIST) with MTT assay procedure in renal transplant recipients.
The dose of calcineurin inhibitors in renal transplantation has been adjusted, based on the therapeutic drug monitoring data. However, the data do not always correlate with clinical drug efficacy. In vitro response of peripheral-blood mononuclear cells to immunosuppressive drugs is reported to correlate with the recipient-response to therapeutic efficacy of the drug. We report, here, usefulness of a lymphocyte immunosuppressant sensitivity test for the estimation of individual drug sensitivity in renal transplant recipients. The LIST we have developed includes MTT assay procedures without the use of radioisotope-labeled compounds, which is convenient for general hospital use. Utilizing this procedure, we compared the pharmacological efficacy between cyclosporine A and tacrolimus in 41 renal transplant recipients.
Sugiyama
Kentaro
K
Division of Pharmacy, Niigata University Medical and Dental Hospital, Niigata City, Niigata, Japan. sugiyama-nii@umin.ac.jp
Arakawa
Keiko
K
Satoh
Hiroshi
H
Saito
Kazuhide
K
Takahashi
Kota
K
Saito
Noriko
N
Hirano
Toshihiko
T
D000328:Adult; D065095:Calcineurin Inhibitors; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006084:Graft Rejection / Q000517:prevention & control; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000494:pharmacology*; D016030:Kidney Transplantation / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016559:Tacrolimus / Q000008:administration & dosage / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D065095:Calcineurin Inhibitors; D007166:Immunosuppressive Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D016572:Cyclosporine; C022616:thiazolyl blue; D016559:Tacrolimus