title: Quorum sensing system lactones do not increase invasiveness of a MexAB-OprM efflux mutant but do play a partial role in Pseudomonas aeruginosa invasion.
abstract: We studied the quorum sensing (QS) system and the related homoserine lactones (HSLs) observing Pseudomonas aeruginosa invasion using the epithelial cell monolayer penetration assay model. Compared to the PAO1 wild-type, the QS mutants, DeltalasI and DeltarhlI, were compromised in their capacity to invade. The decreased invasiveness of DeltarhlI was restored by adding 100 microM exogenous C(4)-HSL. However, the decreased invasiveness of an efflux mutant, DeltamexAB-oprM, was not restored in the presence of exogenous HSLs. The QS system partially plays a role in P. aeruginosa invasion; however, C(4)-HSL and 3-O-C(12)-HSL are not the essential determinants for invasiveness for P. aeruginosa.
authors.affiliation: Department of Laboratory Medicine, Nagasaki University School of Medicine and Dentistry, Nagasaki, Nagasaki 852-850, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kondo
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Hirakata
authors.forename: Naomasa
authors.initials: N
authors.lastname: Gotoh
authors.forename: Kazuko
authors.initials: K
authors.lastname: Fukushima
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ohno
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Higashiyama
authors.forename: Yoshitugu
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Kiyoharu
authors.initials: K
authors.lastname: Nishide
authors.forename: Manabu
authors.initials: M
authors.lastname: Node
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Yamada
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
authors.forename: Shimeru
authors.initials: S
authors.lastname: Kamihira
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004285:Dogs; D008025:Ligases / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C081237:LasI protein, Pseudomonas aeruginosa; D026901:Membrane Transport Proteins; C118598:MexA protein, Pseudomonas aeruginosa; C118597:MexB protein, Pseudomonas aeruginosa; C402263:OprM protein, Pseudomonas aeruginosa; D014157:Transcription Factors; C088386:homoserine lactone; D008025:Ligases; C096955:RHLI protein, Pseudomonas aeruginosa; D015107:4-Butyrolactone