title: Studies of Ca2+ ATPase (SERCA) inhibition.
abstract: The Ca(2+) transport ATPase of intracellular membranes (SERCA) can be inhibited by a series of chemical compounds such as Thapsigargin (TG), 2,5-di(tert-butyl)hydroquinone (DBHQ) and 1,3-dibromo-2,4,6-tris (methyl-isothio-uronium) benzene (Br(2)-TITU). These compounds have specific binding sites in the ATPase protein, and different mechanisms of inhibition. On the other hand, SERCA gene silencing offers a convenient and specific method for suppression of SERCA activity in cells. The physiological and pharmacological implications of SERCA inhibition are discussed.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. ginesi@umaryland.edu
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Inesi
authors.forename: Suming
authors.initials: S
authors.lastname: Hua
authors.forename: Cheng
authors.initials: C
authors.lastname: Xu
authors.forename: Hailun
authors.initials: H
authors.lastname: Ma
authors.forename: Malini
authors.initials: M
authors.lastname: Seth
authors.forename: Anand M
authors.initials: AM
authors.lastname: Prasad
authors.forename: Carlota
authors.initials: C
authors.lastname: Sumbilla
mesh_terms: D001665:Binding Sites; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020868:Gene Silencing / Q000187:drug effects; D034741:RNA, Small Interfering / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D034741:RNA, Small Interfering; D000252:Calcium-Transporting ATPases