title: Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: use for comparison of antigen efficacy.
abstract: Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5 degrees C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development.
authors.affiliation: Department of Cellular Biology, University of Georgia, Athens, USA. zsr3@cdc.gov
authors.forename: Christina M
authors.initials: CM
authors.lastname: Scheel
authors.forename: Sukwan
authors.initials: S
authors.lastname: Handali
authors.forename: Edneia C
authors.initials: EC
authors.lastname: Bueno
authors.forename: Azra
authors.initials: A
authors.lastname: Khan
authors.forename: Kathy
authors.initials: K
authors.lastname: Hancock
authors.forename: Armando E
authors.initials: AE
authors.lastname: Gonzalez
authors.forename: Hector H
authors.initials: HH
authors.lastname: Garcia
authors.forename: Robert H
authors.initials: RH
authors.lastname: Gilman
authors.forename: Victor C W
authors.initials: VC
authors.lastname: Tsang
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D012015:Reference Standards; D041201:Taenia solium / Q000276:immunology; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G