Calibration and application of an X-ray image intensifier/ charge-coupled device detector for monochromatic macromolecular crystallography.
Charge-coupled device (CCD)-based X-ray detectors allow data to be collected much more quickly (approximately 10 times) than with current on-line imaging-plate systems. At the ESRF, X-ray image intensifier/CCD detector systems have been developed. These have great potential as fast read-out detectors for macromolecular and other forms of crystallography. They are relatively large sensitive X-ray detectors but have two inherent weaknesses: convex detection surfaces leading to spatial distortion and non-uniformity of intensity response, and susceptibility to small changes in magnetic fields. A large improvement has been made to the accuracy obtained by non-uniformity of response calibration and correction, using fluorescence from doped lithium borate glasses. Monochromatic macromolecular crystallography demonstration experiments with external user groups have shown that high-quality results may be obtained under real experimental conditions.
Hammersley
A P
AP
ESRF, BP 220, 38043 Grenoble, France.
Brown
K
K
Burmeister
W
W
Claustre
L
L
Gonzalez
A
A
McSweeney
S
S
Mitchell
E
E
Moy
J P
JP
Svensson
S O
SO
Thompson
A W
AW