Age-dependent oxidative stress-induced DNA damage in Down's lymphocytes.
The aim of the present study was to investigate the oxidative status of lymphocytes from children (n=7) and adults (n=18) with Down's syndrome (DS). The basal oxidative condition, the vulnerability to in vitro hydrogen peroxide exposure, and the repair capacity were measured by means of the damage-specific alkaline comet assay. Significantly and age-independently elevated numbers of single strand breaks and oxidized bases (pyrimidines and purines) were found in the nuclear DNA of the lymphocytes in the DS group in the basal condition. These results may support the role of an increased level of endogenous oxidative stress in DS and are similar to those previously demonstrated in Alzheimer's disease. In the in vitro oxidative stress-induced state, a markedly higher extent of DNA damage was observed in DS children as compared with age- and gender-matched healthy controls, suggesting that young trisomic lymphocytes are more sensitive to oxidative stress than normal ones. However, the repair ability itself was not found to be deteriorated in either DS children or DS adults.
Zana
Marianna
M
Department of Psychiatry, Alzheimer's Disease Research Center, Faculty of Medicine, Albert Szent-Györgyi Center for Medical and Pharmaceutical Sciences, University of Szeged, 6 Semmelweis St., Szeged, H-6725, Hungary. mzana@freemail.hu
Szécsényi
Anita
A
Czibula
Agnes
A
Bjelik
Annamária
A
Juhász
Anna
A
Rimanóczy
Agnes
A
Szabó
Krisztina
K
Vetró
Agnes
A
Szucs
Péter
P
Várkonyi
Agnes
A
Pákáski
Magdolna
M
Boda
Krisztina
K
Raskó
István
I
Janka
Zoltán
Z
Kálmán
János
J
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000544:Alzheimer Disease / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D002648:Child; D020552:Comet Assay; D004249:DNA Damage / Q000235:genetics / Q000502:physiology*; D004260:DNA Repair / Q000235:genetics / Q000502:physiology; D004314:Down Syndrome / Q000235:genetics / Q000473:pathology*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress