[Study on tissue culture of Bolbostemma paniculatum].
OBJECTIVE
To study the method of callus induction and culturing of Bolbostemma paniculatum and its relationship with accumulation of tubeimoside.


METHODS
Stem and leaf were selected as explants. The effects of growth substances and their combinations at different concentrations on callus formation and propagation on MS and B5 basic medium were studied.


RESULTS
The optimal compositions of medium that induced calli from stems and leaves were the MS medium supplemented with 2,4-D 2.0 mg x L(-1), NAA 0.5 mg x L(-1) and BA 1.0 mg x L(-1) and the MS medium supplemented with 2,4-D 0.5 mg x L(-1) and NAA 2.0 mg x L(-1), respectively. The calli cultured on the MS medium supplemented with 2,4-D 2.0 mg x L(-1), NAA 0.5 mg x L(-1) and BA 1.0 mg x L(-1) were optimal for the accumulation of tubeimoside. A uniform and stable callus line could be formed from the calli cultured repeatedly on B5 medium supplemented with 2,4-D 2.0 mg x L(-1), NAA 0.5 mg x L(-1) and BA 1.0 mg x L(-1).


CONCLUSIONS
Using stem and leaf as explants, the method of the callus induction and culture of B. paniculatum was established and it could provide some references for the production of tubeimoside by tissue and cell culture.
Li
Cui-qin
CQ
Key Laboratory of Medicinal Plant Resource and Natural Pharmaceutical Chemistry of Ministry of Education, Shaanxi Normal University, Xi'an, China.
Wang
Zhe-zhi
ZZ
D019662:Cucurbitaceae / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D012503:Saponins / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods*; D014315:Triterpenes / Q000378:metabolism
D003470:Culture Media; D010937:Plant Growth Regulators; D012503:Saponins; D014315:Triterpenes; C051084:tubeimoside I