Application of a column selection system and DryLab software for high-performance liquid chromatography method development.
This paper describes a strategy for the development of chromatographic methods for drug candidates based upon the use of simple MS compatible mobile phases and optimization of the chromatographic selectivity through variations of the stationary phase and mobile phase pH. The strategy employs an automated column selection system and a series of HPLC columns, varying in hydrophobicity and silanol activity, in combination with DryLab software to develop chromatographic methods for the separation of mixtures of bupivacaine and its metabolites; acidic, basic, and neutral compounds; and atenolol, nitrendipine, and their degradation products.
Krisko
Ryan M
RM
Department of Chemistry, University of Kansas, Mallott Hall, Lawrence, KS 66045, USA.
McLaughlin
Kieran
K
Koenigbauer
Michael J
MJ
Lunte
Craig E
CE
D001262:Atenolol / Q000032:analysis / Q000737:chemistry; D002045:Bupivacaine / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003198:Computer Simulation; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012984:Software; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013237:Stereoisomerism
D004364:Pharmaceutical Preparations; D001262:Atenolol; D002045:Bupivacaine