Effects of Ginkgolide on the development of NOS and AChE positive neurons in the embryonic basal forebrain.
Extract of Ginkgo biloba (EGb) has been therapeutically used for several decades to increase peripheral and cerebral blood flow so as to prevent cardiovascular and neurovascular diseases. However, the role of EGb in neuroprotective effects has received much attention recently. In this study, we investigated the effect of EGb on the development of NOS and AChE positive neurons in the rat embryonic basal forebrain. The results showed that treated with EGb, the OD of MTT staining analysis, and the numbers, the cell sizes and circumferences of NOS and AChE positive neurons were greatly promoted. These data suggest that EGb had similar effects of the neurotrophins such as NGF and BDNF in promoting the development of NOS and AChE positive neurons in the rat embryonic basal forebrain.
Jin
Guo-Hua
GH
Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Nantong University, Nantong 226001, China. jguohua@ntu.edu.cn
Huang
Zhen
Z
Tan
Xue-Feng
XF
Tian
Mei-Ling
ML
Zhang
Xin-Hua
XH
Qin
Jian-Bing
JB
Xu
Hui-Jun
HJ
Yew
D T
DT
Mak
Y T
YT
D000105:Acetyl Coenzyme A / Q000032:analysis*; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D018929:Cell Culture Techniques; D048429:Cell Size / Q000187:drug effects; D003124:Colorimetry; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020441:Ginkgo biloba; D046934:Ginkgolides / Q000494:pharmacology*; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000032:analysis*; D016548:Prosencephalon / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D051381:Rats
D019208:Brain-Derived Neurotrophic Factor; D004365:Drugs, Chinese Herbal; D046934:Ginkgolides; D000105:Acetyl Coenzyme A; D020932:Nerve Growth Factor; D019001:Nitric Oxide Synthase