An oligonucleotide microarray study on gene expression profile in mouse testis of experimental cryptorchidism.
To investigate the germ cell apoptosis under body temperature in testis, we analyzed the gene expression patterns on day 1, day 4, day 7, day 14, day 28 and normal control adult mouse testis after experimental cryptorchidism (EC) using Affymetrix MOE430A microarray. Our data showed that EC led to the oxidative stress and gene expression fluctuation in the first 28 days, both of which were highly coincident in timing. Cryptorchid testis showed more effective antioxidative capability in the first 4 days, and suddenly lowered the capability from day 5 on, then gradually restored the antioxidation from day 10 to day 14, and turned to worse on day 28 again. The extensive high gene expression on day 4 after EC and the up-rising of oxidative stress level on day 5 and the abrupt down-regulation of the gene on day 7 were closely related. From the chip data, we have found that the high level of reactive oxidative species (ROS) was not only related to the dysfunction or abnormality of the direct origin of ROS generation, but also related to the abnormality of the more upstream physiological events in energy metabolism, lipid metabolism. The selective regulation of metabolic substrate transporter in different cell population implied the existence of various regulation of the selective signal pathways among different cell populations by EC.
Li
Yin-Chuan
YC
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
Hu
Xiao-Qian
XQ
Xiao
Li-Juan
LJ
Hu
Zhao-Yuan
ZY
Guo
Jian
J
Zhang
Ke-Ying
KY
Song
Xin-Xin
XX
Liu
Yi-Xun
YX
D000818:Animals; D017209:Apoptosis; D003456:Cryptorchidism / Q000235:genetics*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005854:Germ Cells / Q000378:metabolism; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013737:Testis / Q000033:anatomy & histology / Q000378:metabolism*
D017382:Reactive Oxygen Species