title: Transcriptomic analysis indicates putative metabolic changes caused by manipulation of phosphorus availability in rice leaves.
abstract: Plants have developed several strategies for coping with phosphorus (P) deficiency. However, the details of the regulation of gene expression of adaptations to low P are still unclear. Using a cDNA microarray, transcriptomic analyses were carried out of the rice genes regulated by P deficiency and P re-supply to P-deficient plants. The OsPI1 gene, which was isolated as the most significant up-regulated gene under -P conditions, was also the most significant down-regulated gene following P re-supply. Many starch metabolism-related genes, as well as several genes for P(i)-liberating enzymes, were up-regulated by -P treatment, suggesting a homeostatic contribution to the P(i) concentration in leaf tissues. mRNAs for glucanases were also induced by P re-supply: these are suspected to play a role in loosening the cell wall compounds. Most of the genes up-regulated by -P treatment were down-regulated by P re-supply, suggesting that their responses were specific to -P conditions. Conversely, the number of genes up-regulated by P re-supply was also larger following P re-supply than in the -P condition. It is proposed that the genes up-regulated by P re-supply play an important role in P acquisition by P-deficient plants.
authors.affiliation: Creative Research Initiative Sousei (CRIS), Hokkaido University, Kita-ku, Sapporo, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Wasaki
authors.forename: Takuro
authors.initials: T
authors.lastname: Shinano
authors.forename: Kazuki
authors.initials: K
authors.lastname: Onishi
authors.forename: Ryoma
authors.initials: R
authors.lastname: Yonetani
authors.forename: Junshi
authors.initials: J
authors.lastname: Yazaki
authors.forename: Fumiko
authors.initials: F
authors.lastname: Fujii
authors.forename: Kanako
authors.initials: K
authors.lastname: Shimbo
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Zempei
authors.initials: Z
authors.lastname: Shimatani
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nagata
authors.forename: Akiko
authors.initials: A
authors.lastname: Hashimoto
authors.forename: Tomoya
authors.initials: T
authors.lastname: Ohta
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sato
authors.forename: Chikako
authors.initials: C
authors.lastname: Miyamoto
authors.forename: Sachiko
authors.initials: S
authors.lastname: Honda
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kojima
authors.forename: Takuji
authors.initials: T
authors.lastname: Sasaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Kishimoto
authors.forename: Shoshi
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Osaki
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*
chemical_list: D010758:Phosphorus