title: Injectable calcium phosphate scaffold and bone marrow graft for bone reconstruction in irradiated areas: an experimental study in rats.
abstract: The purpose of this study was to assess the possibilities for bone reconstruction of an injectable calcium phosphate scaffold (ICPS) associated with a bone marrow (BM) graft after irradiation in a rat model. External irradiation was delivered to 12 out of 27 inbred rats. Three weeks later, four osseous defects were created per animal and were kept empty or filled with either ICPS alone, BM graft alone or with a mixture of BM and ICPS. Three weeks after implantation, bone specimens were studied under light microscopy and by scanning electron microscopy. Filling irradiated defects with ICPS alone was not accompanied by the formation of new bone. The BM graft associated with ICPS significantly increased ceramic degradation (p<0.01) and bone ingrowth (p<0.01) in the irradiated areas. The results are evidence for the meaning of the BM in driving the bone repair in irradiated animals.
authors.affiliation: INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 Place Alexis Ricordeau, F44042 Nantes Cedex, France. elerouxel@yahoo.fr
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Lerouxel
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.forename: Bernard
authors.initials: B
authors.lastname: Giumelli
authors.forename: Anne
authors.initials: A
authors.lastname: Moreau
authors.forename: Paul
authors.initials: P
authors.lastname: Pilet
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guicheux
authors.forename: Pierre
authors.initials: P
authors.lastname: Corre
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Bouler
authors.forename: Guy
authors.initials: G
authors.lastname: Daculsi
authors.forename: Olivier
authors.initials: O
authors.lastname: Malard
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D016026:Bone Marrow Transplantation; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000008:administration & dosage*; D016025:Bone Transplantation / Q000379:methods*; D001842:Bone and Bones / Q000648:ultrastructure; D002130:Calcium Phosphates / Q000008:administration & dosage*; D002516:Ceramics / Q000378:metabolism; D005260:Female; D007267:Injections; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C020243:calcium phosphate