Amplification and overexpression of the cyclin D1 gene in head and neck squamous cell carcinoma.
Aims-To determine cyclin D1 gene amplification and expression levels in head and neck squamous cell carcinoma (HNSCC) patients.Methods-Total RNA and genomic DNA were isolated from 40 samples of HNSCC tissue and matched normal tissue and were hybridised with a cyclin D1 cDNA probe. Northern and Southern analyses were used to detect mRNA overexpression and cyclin D1 gene amplification, respectively.Results-15 of the 40 HNSCC samples examined (38%) showed cyclin D1 gene amplification. Of these 15 samples, all 13 from which RNA was available showed increased cyclin D1 expression.Conclusions-HNSCC patients with both amplification and overexpression of the cyclin D1 gene are at greater risk than patients who showed no cyclin D1 gene amplification; amplification and over-expression of the cyclin D1 gene may play an important role in the progression of HNSCC and in clinical outcome.
Wang
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Department of Cell Biology, Neurobiology and Anatomy, College of Medicine, University of Cincinnati, 231 Bethesda Avenue, Cincinnati, Ohio 45267-0521, USA.
Pavelic
Z P
ZP
Li
Y Q
YQ
Wang
L
L
Gleich
L
L
Radack
K
K
Gluckman
J L
JL
Stambrook
P J
PJ