title: Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags.
abstract: Intrahepatic cholangiocarcinoma (ICC), a malignant tumor derived from the bile duct epithelium, is one of the leading causes of death from cancer, worldwide. However, the mechanisms related to it remain largely unknown. In this study, an analysis of the gene expression profiles for ICC was done using the frequency of the ESTs obtained from nine cDNA libraries that constructed from 4 ICC cell lines and 4 normal liver tissues. One hundred and thirty-seven genes were identified as being either up- or down-regulated in human ICC cells. Thirty genes were randomly selected to confirm their differential expression in 4 human ICC cell lines and 5 ICC tissues compared to normal liver tissues by semi-quantitative RT-PCR. Among these genes, ANXA1, ANXA2, AMBP, and SERPINC1 were further verified by immunohistochemical analyses. In conclusion, these identified genes represent potential biomarkers for ICC and represent potential targets for elucidating the molecular mechanisms that are associated with ICC.
authors.affiliation: Laboratory of Human Genomics, Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Wang
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Yoon
authors.forename: Jung-Hwa
authors.initials: JH
authors.lastname: Oh
authors.forename: Yeo-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Mirang
authors.initials: M
authors.lastname: Kim
authors.forename: Jeong-Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Sang-Soon
authors.initials: SS
authors.lastname: Byun
authors.forename: Jin Ok
authors.initials: JO
authors.lastname: Yang
authors.forename: Joo Heon
authors.initials: JH
authors.lastname: Kim
authors.forename: Dae-Ghon
authors.initials: DG
authors.lastname: Kim
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Yeom
authors.forename: Hyang-Sook
authors.initials: HS
authors.lastname: Yoo
authors.forename: Yong Sung
authors.initials: YS
authors.lastname: Kim
authors.forename: Nam-Soon
authors.initials: NS
authors.lastname: Kim
mesh_terms: D001650:Bile Duct Neoplasms / Q000235:genetics*; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000235:genetics*; D015536:Down-Regulation; D020224:Expressed Sequence Tags; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D020022:Genetic Predisposition to Disease; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor