Differential acetylcholinesterase activity in rat cerebrum, cerebellum and hypothalamus.
Acetylcholinesterase (AChE) has been purified from three different regions of rat brain using Sephadex G 200 column. SDS PAGE (6%) showed single band for the purified AChE fractions. Purified and lyophilized AChE from different (NH4)2SO4 precipitated fractions of three brain parts were utilized for in vitro enzyme kinetics using Dimethoate (Dmt) as inhibitor. K(m) values for cerebellum and hypothalamus were almost similar whereas cerebrum showed a different K(m) value compared to other two regions. With the drug Rivastigmine it was found that % G1 and G4 forms of AChE in three different parts of brain are different.
Roy
Rini
R
Department of Microbiology, Lady Brabourne College, P1/2 Suhrawardy Avenue, Kolkata 700 017, India.
Chaudhuri
Aditi Nag
AN
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002531:Cerebellum / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007031:Hypothalamus / Q000201:enzymology*; D008297:Male; D051381:Rats; D013687:Telencephalon / Q000201:enzymology*
D000110:Acetylcholinesterase