title: Immunohistochemically detectable p53 and mdm-2 oncoprotein expression in colorectal carcinoma: prognostic significance.
abstract: Aims-To investigate the correlation between the expression of the p53 and mdm-2 oncoproteins and to assess their prognostic value in colorectal cancer.Methods-Using a polyclonal (CM1) and a monoclonal antibody directed against p53 and mdm-2, respectively, these oncoproteins were stained immunohistochemically in 109 colorectal adenocarcinomas.Results-p53 was detected in less than 10% of tumour cells in 11 of 109 adenocarcinomas, in 10-50% of tumour cells, in 17 of 109 adenocarcinomas, and in more than 50% of tumour cells in 32 of 109 adenocarcinomas. Expression of mdm-2 was detected in 22 of 109 (20%) cases investigated, of which 19 showed concomitant p53 expression. In most cases mdm-2 immunoreactivity was strongly associated with a small proportion of p53 positive tumour cells. Both p53 and mdm-2 expression lacked statistical significance when correlated with common staging and grading parameters.Conclusions-Detection of p53 and mdm-2 oncoprotein expression, detected using immunohistochemistry, is of no prognostic value in colorectal cancer. However, the close correlation between mdm-2 immunoreactivity and the proportion of p53 positive cells provides further evidence that the mdm-2 gene product interacts with p53 protein.
authors.affiliation: Gerhard Domagk Institute of Pathology, University of Münster, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Ofner
authors.forename: H
authors.initials: H
authors.lastname: Maier
authors.forename: B
authors.initials: B
authors.lastname: Riedmann
authors.forename: P
authors.initials: P
authors.lastname: Holzberger
authors.forename: M
authors.initials: M
authors.lastname: Nogler
authors.forename: M
authors.initials: M
authors.lastname: Tötsch
authors.forename: A
authors.initials: A
authors.lastname: Bankfalvi
authors.forename: G
authors.initials: G
authors.lastname: Winde
authors.forename: W
authors.initials: W
authors.lastname: Böcker
authors.forename: K W
authors.initials: KW
authors.lastname: Schmid