title: HA/alginate hybrid composites prepared through bio-inspired nucleation.
abstract: Poorly crystalline apatite has been directly nucleated on self-assembling alginate chains by neutralization synthesis to obtain a biomimetic artificial bone-like composite. It has been observed that in preparing HA/alginate composites, Ca2+ ions present on the apatitic surface cross-link the alginate chains to produce a material with different morphology and thermal stability, both functions of the HA/alginate weight ratio. In vitro tests were performed on different samples in terms of both the HA/alginate ratio and synthesis temperature. All the samples were cultured for seven days with MG63 osteoblast-like cells and then underwent morphological and biochemical analyses (MTT and ALP tests). Scaffolds showed a different solubility into the culture media, which was related to the temperature of synthesis and to the HA/alginate ratio. All our data confirm the ability of the tested materials to favour cell growth and to maintain their osteoblastic functionality, at least during the examined period.
authors.affiliation: Institute of Science and Technology for Ceramics ISTEC-CNR, via Granarolo 64, I-48018 Faenza (RA), Italy. tampieri@istec.cnr.it
authors.forename: A
authors.initials: A
authors.lastname: Tampieri
authors.forename: M
authors.initials: M
authors.lastname: Sandri
authors.forename: E
authors.initials: E
authors.lastname: Landi
authors.forename: G
authors.initials: G
authors.lastname: Celotti
authors.forename: N
authors.initials: N
authors.lastname: Roveri
authors.forename: M
authors.initials: M
authors.lastname: Mattioli-Belmonte
authors.forename: L
authors.initials: L
authors.lastname: Virgili
authors.forename: F
authors.initials: F
authors.lastname: Gabbanelli
authors.forename: G
authors.initials: G
authors.lastname: Biagini
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D017886:Durapatite; D000469:Alkaline Phosphatase