title: Ionic channels and conductance-based models for hypothalamic neuronal thermosensitivity.
abstract: Thermoregulatory responses are partially controlled by the preoptic area and anterior hypothalamus (PO/AH), which contains a mixed population of temperature-sensitive and insensitive neurons. Immunohistochemical procedures identified the extent of various ionic channels in rat PO/AH neurons. These included pacemaker current channels [i.e., hyperpolarization-activated cyclic nucleotide-gated channels (HCN)], background potassium leak channels (TASK-1 and TRAAK), and transient receptor potential channel (TRP) TRPV4. PO/AH neurons showed dense TASK-1 and HCN-2 immunoreactivity and moderate TRAAK and HCN-4 immunoreactivity. In contrast, the neuronal cell bodies did not label for TRPV4, but instead, punctate labeling was observed in traversing axons or their terminal endings. On the basis of these results and previous electrophysiological studies, Hodgkin-Huxley-like models were constructed. These models suggest that most PO/AH neurons have the same types of ionic channels, but different levels of channel expression can explain the inherent properties of the various types of temperature-sensitive and insensitive neurons.
authors.affiliation: School of Mathematics and Statistics, University of Sydney, Sydney, Australia.
authors.forename: Martin
authors.initials: M
authors.lastname: Wechselberger
authors.forename: Chadwick L
authors.initials: CL
authors.lastname: Wright
authors.forename: Georgia A
authors.initials: GA
authors.lastname: Bishop
authors.forename: Jack A
authors.initials: JA
authors.lastname: Boulant
mesh_terms: D000200:Action Potentials; D000818:Animals; D001831:Body Temperature / Q000502:physiology*; D004553:Electric Conductivity; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000166:cytology*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007473:Ion Channels