title: One-tube-PCR technique for CCL2, CCL3, CCL4 and CCL5 applied to fine needle aspiration biopsies shows different profiles in autoimmune and non-autoimmune thyroid disorders.
abstract: Autoimmune thyroid diseases are characterized by lymphocytic infiltration of the thyroid gland. Chemokines are crucial in the recruitment of lymphocytes and might play an important role in the pathogenesis of autoimmune thyroid disease. The aim of this study was to test the feasibility of analysing by one-tube reverse-transcriptase polymerase chain reaction (RT-PCR) technique CC chemokine profiles in samples obtained by fine needle aspiration biopsy (FNAB). In 27 out of 35 (77%) samples, the material was sufficient for analysis and in 16 (59%) chemokines were detected, thus demonstrating the potential of this technique. Moreover, even in this small group, a statistically significant increase of CCL3 and CCL4 was found in samples from patients with autoimmune thyroid disease as compared to those with multinodular goiter. Chemokine profile measured by improved multiamplification techniques in FNAB thyroid samples may become a useful complementary tool for the management of thyroid autoimmune disease as it constitutes a source of data for research of their pathogenesis.
authors.affiliation: Immunology (Laboratory of Immunology for Research Applied to Diagnosis (LIRAD), Blood and Tissue Bank, Institute for Health Sciences Research Germans Trias i Pujol, Badalona, Barcelona, Spain.
authors.forename: X
authors.initials: X
authors.lastname: Ferrer-Francesch
authors.forename: P
authors.initials: P
authors.lastname: Caro
authors.forename: L
authors.initials: L
authors.lastname: Alcalde
authors.forename: M P
authors.initials: MP
authors.lastname: Armengol
authors.forename: Y
authors.initials: Y
authors.lastname: Ashhab
authors.forename: A
authors.initials: A
authors.lastname: Lucas-Martín
authors.forename: E M
authors.initials: EM
authors.lastname: Martínez-Cáceres
authors.forename: M
authors.initials: M
authors.lastname: Juan
authors.forename: R
authors.initials: R
authors.lastname: Pujol-Borrell
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001707:Biopsy, Needle; D018932:Chemokine CCL2 / Q000032:analysis; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D019742:Chemokines, CC / Q000032:analysis*; D005260:Female; D006801:Humans; D007453:Iodide Peroxidase / Q000276:immunology; D019402:Macrophage Inflammatory Proteins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011989:Receptors, Thyrotropin / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D013954:Thyroglobulin / Q000276:immunology; D013959:Thyroid Diseases / Q000175:diagnosis*; D013967:Thyroiditis, Autoimmune / Q000175:diagnosis
chemical_list: C111835:CCL2 protein, human; C066507:CCL4 protein, human; C483024:CCL5 protein, human; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D011989:Receptors, Thyrotropin; D013954:Thyroglobulin; D007453:Iodide Peroxidase