PCR-identification of Dunaliella salina (Volvocales, Chlorophyta) and its growth characteristics.
The saline pond microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's (basal) medium under laboratory conditions was confirmed by amplifying the chromosomal DNA of the microalga by PCR with specific primers MA1 and MA2. Seaweed extracts obtained from Sargassum wightii and Ulva lactuca were amended separately at 1.0%, 1.5%, 2.0% and 2.5% levels to the basal medium in order to assess their potential on the growth and concentration of pigments, viz. Chl a, Chl b and beta-carotene of the alga. beta-Carotene was isolated and visible absorption spectrum was taken at 443 and 475 nm confirmed the presence of 9-cis-beta-carotene and all-trans-beta-carotene isomers. Maximum yield, highest division rate (mu) and highest pigment concentrations were observed in the cells grown in 1.5% S. wightii and 2.0% U. lactuca amended medium and these cells were subjected to DAPI staining. The results of epifluorescence microscopy and image analysis revealed a significant enhancement of the cell and nuclear area of the microalgae.
Raja
Rathinam
R
Centre for Advanced Studies in Botany, Guindy Campus, University of Madras, Chennai 600 025, India. rraja307@yahoo.co.in
Hema Iswarya
Shanmugam
S
Balasubramanyam
Dakshanamoorthy
D
Rengasamy
Ramasamy
R
D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D000460:Chlorophyta / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry / Q000235:genetics; D007194:India; D007218:Industrial Microbiology; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010936:Plant Extracts / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012624:Seaweed / Q000737:chemistry; D014871:Water Microbiology; D019207:beta Carotene / Q000378:metabolism
D010936:Plant Extracts; D019207:beta Carotene; D002734:Chlorophyll; C037184:chlorophyll b; D004247:DNA; D000077194:Chlorophyll A