[The role of proton pump and potassium channels of plasma membrane in regulation of cellulose synthesis in plants].
A study was made of the influence of two activators of plasma membrane proton pump [indole-3-acetic acid (IAA) and fusicoccin (EC)] and of the blocker of potassium channels of outward direction [tetraethylammonium chloride (TEA)] on exogenous [U-14C]glucose incorporation into cellulose fraction of cell wall, and on the value of plasmalemma membrane potential. It has been shown that IAA and FC exert different influences on the intensity of [U-14C]glucose incorporation into cellulose fraction: IAA activates, while FC inhibits incorporation intensity. A conclusion is made that differences in affects of IAA and FC on the intensity of cellulose synthesis at the plasma membrane level may be due to the fact that the activating effect of IAA on plasma membrane proton pump involves activation of the inward direction potassium channels, whereas that of FC, on the contrary, is associated with their blocking. Under the action of TEA, the intensity of incorporation of radioactively labeled glucose was increased. Apparently, the role of plasma membrane in regulation of the intensity of cellulose synthesis may be associated with not only the activity of proton pump on plasma membrane, but also the functional condition of potassium channels of this membrane.
Belova
L P
LP
Velikanov
G A
GA
Il'ina
T M
TM
D002462:Cell Membrane / Q000378:metabolism* / Q000502:physiology; D002478:Cells, Cultured; D002482:Cellulose / Q000096:biosynthesis*; D006027:Glycosides / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology; D008564:Membrane Potentials; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000378:metabolism / Q000648:ultrastructure; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels / Q000502:physiology*; D017494:Proton Pumps / Q000502:physiology*; D036226:Seedlings / Q000378:metabolism / Q000648:ultrastructure; D019789:Tetraethylammonium / Q000494:pharmacology; D014908:Triticum / Q000378:metabolism* / Q000648:ultrastructure
D006027:Glycosides; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D017494:Proton Pumps; C007808:fusicoccin; D019789:Tetraethylammonium; C030737:indoleacetic acid; D002482:Cellulose