title: Association study of genetic variants of pro-inflammatory chemokine and cytokine genes in systemic lupus erythematosus.
abstract: BACKGROUND
  Several lines of evidence suggest that chemokines and cytokines play an important role in the inflammatory development and progression of systemic lupus erythematosus. The aim of this study was to evaluate the relevance of functional genetic variations of RANTES, IL-8, IL-1alpha, and MCP-1 for systemic lupus erythematosus.
  
  
  METHODS
  The study was conducted on 500 SLE patients and 481 ethnically matched healthy controls. Genotyping of polymorphisms in the RANTES, IL-8, IL-1alpha, and MCP-1 genes were performed using a real-time polymerase chain reaction (PCR) system with pre-developed TaqMan allelic discrimination assay.
  
  
  RESULTS
  No significant differences between SLE patients and healthy controls were observed when comparing genotype, allele or haplotype frequencies of the RANTES, IL-8, IL-1alpha, and MCP-1 polymorphisms. In addition, no evidence for association with clinical sub-features of SLE was found.
  
  
  CONCLUSIONS
  These results suggest that the tested functional variation of RANTES, IL-8, IL-1alpha, and MCP-1 genes do not confer a relevant role in the susceptibility or severity of SLE in the Spanish population.
authors.affiliation: Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Granada, Spain. elena@ipb.csic.es
authors.forename: Elena
authors.initials: E
authors.lastname: Sánchez
authors.forename: José M
authors.initials: JM
authors.lastname: Sabio
authors.forename: José L
authors.initials: JL
authors.lastname: Callejas
authors.forename: Enrique
authors.initials: E
authors.lastname: de Ramón
authors.forename: Rosa
authors.initials: R
authors.lastname: Garcia-Portales
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: García-Hernández
authors.forename: Juan
authors.initials: J
authors.lastname: Jiménez-Alonso
authors.forename: Ma Francisca
authors.initials: MF
authors.lastname: González-Escribano
authors.forename: Javier
authors.initials: J
authors.lastname: Martín
authors.forename: Bobby P
authors.initials: BP
authors.lastname: Koeleman
mesh_terms: D000328:Adult; D018932:Chemokine CCL2 / Q000235:genetics; D018946:Chemokine CCL5 / Q000235:genetics; D018925:Chemokines / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007249:Inflammation / Q000235:genetics; D007375:Interleukin-1 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide
chemical_list: D018932:Chemokine CCL2; D018946:Chemokine CCL5; D018925:Chemokines; D007375:Interleukin-1; D016209:Interleukin-8; D007378:Interleukins