Precision study on capillary electrophoresis methods for metacycline.
A CE method for metacycline (MTC) determination was investigated in an inter-laboratory experiment. Many problems were encountered in this study, most of which were related to the transfer of the method to different CE equipment. The reported problems could be classified into different categories: problems related to the precision, to the parameters in the protocol, and to the MTC peak shape. As the peak shape problem was partially responsible for the poor precision, a new CE method was developed in order to obtain a good MTC peak shape on all equipment. The precision of this new method for MTC determination was examined in an intermediate precision study, where the influence of the factors "time" and "equipment" was investigated. Although the new method could be transferred to different instruments, the precision remained poor mainly due to the contributions of the between-replicate and the between-injection variances.
Thi
Thao Do
TD
Laboratory of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Leuven, Belgium.
Pomponio
Romeo
R
Gotti
Roberto
R
Saevels
Jan
J
Van Hove
Ben
B
Van Ael
Willy
W
Matthijs
Nele
N
Vander Heyden
Yvan
Y
Djan'geing'a
Roland Marini
RM
Chiap
Patrice
P
Hubert
Philippe
P
Crommen
Jacques
J
Fabre
Huguette
H
Dehouck
Pieter
P
Hoogmartens
Jos
J
Van Schepdael
Ann
A
D000900:Anti-Bacterial Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008690:Methacycline / Q000032:analysis*; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D008690:Methacycline