title: Activity of lysosomal exoglycosidases in human gliomas.
abstract: There is a lot of data suggesting that modifications of cell glycoconjugates may be important in progression of cancer. In the present work we studied activities of lysosomal exoglycosidases: beta-hexosaminidase and its isoenzymes A and B, beta-galactosidase and alpha-mannosidase, in human gliomas. Enzyme activity was determined spectrophotometrically based on the release of p-nitrophenol from p-nitrophenyl-derivative of appropriate sugars. The activities of the exoglycosidases tested were significantly higher in malignant glial tumors than in control tissue (normal brain tissue) and non-glial tumors. The highest activities of exoglycosidases were observed in high-grade gliomas, and a positive correlation of enzyme activities and degree of malignancy was noted. Our results suggest that lysosomal exoglycosidases may participate in the progression and dynamical development of glial tumors.
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University of Bialystok, ul. Mickiewicza 2A , 15-089, Bialystok, Poland. pwielg@amb.edu.pl
authors.forename: P
authors.initials: P
authors.lastname: Wielgat
authors.forename: U
authors.initials: U
authors.lastname: Walczuk
authors.forename: S
authors.initials: S
authors.lastname: Szajda
authors.forename: M
authors.initials: M
authors.lastname: Bień
authors.forename: L
authors.initials: L
authors.lastname: Zimnoch
authors.forename: Z
authors.initials: Z
authors.lastname: Mariak
authors.forename: K
authors.initials: K
authors.lastname: Zwierz
mesh_terms: D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000201:enzymology* / Q000473:pathology; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D043323:alpha-Mannosidase / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; D043323:alpha-Mannosidase; D001619:beta-N-Acetylhexosaminidases