Isolation and expression analysis of plastidic glucose-6-phosphate dehydrogenase gene from rice (Oryza sativa L.).
Glucose-6-phosphate dehydrogenase is a rate-limiting enzyme of pentose phosphate pathway, existing in cytosolic and plastidic compartments of higher plants. A novel gene encoding plastidic glucose-6-phosphate dehydrogenase was isolated from rice (Oryza sativa L.) and designated OsG6PDH2 in this article. Through semiquantitative RT-PCR approach it was found that OsG6PDH2 mRNA was weakly expressed in rice leaves, stems, immature spikes or flowered spikes, and a little higher in roots. However, the expression of OsG6PDH2 in rice seedlings was significantly induced by dark treatment. The complete opening reading frame (ORF) of OsG6PDH2 was inserted into pET30a (+), and expressed in Escherichia coli strain BL21 (DE3). The enzyme activity assay of transformed bacterial cells indicated that OsG6PDH2 encoding product had a typical function of glucose-6-phosphate dehydrogenase.
Hou
Fu-Yun
FY
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China.
Huang
Ji
J
Lu
Ju-Fei
JF
Wang
Zhou-Fei
ZF
Zhang
Hong-Sheng
HS
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D018517:Plant Roots / Q000201:enzymology; D018087:Plastids / Q000201:enzymology / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D036226:Seedlings / Q000201:enzymology / Q000235:genetics
D018744:DNA, Plant; D012333:RNA, Messenger; D005954:Glucosephosphate Dehydrogenase