[HSF1 inhibits heat stress-induced apoptosis in Raw264.7 macrophages].
OBJECTIVE
To observe the effect of heat shock factor 1 (HSF1) on heat stress-induced apoptosis in Raw264.7 macrophages.


METHODS
Raw264.7 cells transfected with pcDNA3.1 and pcDNA3.1-HSF1 were exposed to heat stress (42.5 degrees C +/- 0.5 degrees C) for 1 h and recovered at 37 degrees C for 6, 9, 12, and 24 h respectively. Flow cytometry (FCM), Hoechst 33258 staining and DNA ladder assays were performed to assess the apoptosis.


RESULTS
After heat stress, FCM showed that apoptotic cells were increased significantly and reached the peak at 9 h in Raw 264.7 cells transfected with pcDNA3.1, and were characterized with classical morphologic changes including apoptotic body and nuclear condensation. Agarose gel electrophoresis showed that "DNA ladder" could be observed clearly at 6, 9, and 12 h after the heat stress. But the overexpression of HSF1 could reduce the number of apoptotic cells and inhibit DNA fragmentation.


CONCLUSIONS
HSF1 can inhibit heat stress-induced apoptosis in Raw264.7 macrophages.
Shun-mei
E
E
Department of Pathophsiology, Xiangya School of Medicine, Central South University, Changsha, China.
Xiao
Wei-min
WM
Wang
Kang-kai
KK
Wang
Qiu-peng
QP
Liu
Mei-dong
MD
Liu
Ke
K
Xiao
Xian-zhong
XZ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D000076249:Heat Shock Transcription Factors; D018869:Heat-Shock Response; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D051381:Rats; D014157:Transcription Factors / Q000494:pharmacology*; D014162:Transfection
D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; C490017:Hsf1 protein, mouse; D014157:Transcription Factors