Laccase activity from the fungus Trametes hirsuta using an air-lift bioreactor.
OBJECTIVE
To produce high laccase activities from the white-rot fungus Trametes hirsuta in an in-house air-lift bioreactor (ALB).


RESULTS
Trametes hirsuta was grown in a 6-l ALB. A fed-batch strategy with glycerol as an addition resulted in maximum laccase activity of 19,400 U l(-1), which was the highest reported from the fungus.


CONCLUSIONS
The ALB configuration with additional glycerol resulted in high laccase activities.


CONCLUSIONS
This study provides useful information on how to produce high concentrations of laccase.
Rodríguez Couto
S
S
Department of Chemical Engineering, University of Vigo, Vigo, Spain. susana.rodriguez@urv.net
Rodríguez
A
A
Paterson
R R M
RR
Lima
N
N
Teixeira
J A
JA
D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D005990:Glycerol / Q000378:metabolism; D042845:Laccase / Q000378:metabolism*
D003470:Culture Media; D042845:Laccase; D005990:Glycerol