Optimization of preparation of DHAQ-loaded PEG-PLGA-PEG nonaparticles using central composite design.
Mitoxantrone (DHAQ)-loaded poly (ethylene glycol)-poly (lactic acid-co-glycolic acid) -poly (ethyleneglycol) (PELGE) nanoparticles (NP) were fabricated using an emulsification/solvent evaporation technique. A central composite design (CCD) was applied to evaluate the joint influence of three formulation variables: the amounts of polymer, concentration of the DHAQ, and the ratio of the organic phase (inner-phase) and the aqueous phase (outer-phase). In this study, we optimize the preparation technology on the basis of the single factor evaluation. The optimal conditions for the preparation of DHAQ-loaded nanoparticle were found to be: the concentration of PELGE was 9 mg/mL, the concentration of inner-phase of DHAQ was 27.5 mg/L, and the ratio of inner-phase/outer-phase was 8.5/1. The results showed that CCD is an ideal technique for formulation studies. The entrapment efficiency ratio (ER) was 90% and particle sizes are less than 500 nm. The nanoparticles, as examined by transmission electron microscopy (TEM), have a smooth and spherical surface. The DHAQ could be loaded into PELGE copolymers. In this study, the DHAQ nanoparticle-polymer delivery system was established by using PELGE polymers as carrier material.
Duan
Yourong
Y
Shanghai Cancer Institute, Cancer Institute of Shanghai JiaoTong University, Shanghai 200032, China.
Xu
Sen
S
Wang
Qi
Q
Liu
Jie
J
Zhang
Zhirong
Z
D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D004655:Emulsions; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D008942:Mitoxantrone / Q000493:pharmacokinetics*; D049329:Nanostructures / Q000648:ultrastructure; D036103:Nanotechnology; D010316:Particle Size; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004655:Emulsions; D011108:Polymers; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D008942:Mitoxantrone