[Application of four ELISA techniques (two for IgM and two for IgG) for serological diagnosis of an outbreak of Q fever].
OBJECTIVE
To evaluate four ELISAs (commercialized by Panbio and Serion) for IgM and IgG detection in an outbreak of Q fever.


METHODS
Fifty-three serum samples corresponding to 29 persons were processed. An indirect immunofluorescence assay was used as the reference method.


RESULTS
The sensitivity and specificity for IgM were 100% and 74% (Panbio), and 91% and 88% (Serion). IgG sensitivity was 72% (Panbio) and 89% (Serion). The specificity of both techniques for IgG was 97%.


CONCLUSIONS
Both ELISAs seem to be good IgM screening methods for use during Q fever outbreaks.
Sanz
Juan Carlos
JC
Laboratorio Regional de Salud Pública, Instituto de Salud Pública, Comunidad de Madrid, Spain. juan.sanz@salud.madrid.org
de los Ríos
Rosa
R
Martín
Fernando
F
Tébar
M Angeles
MA
Jado
Isabel
I
Anda
Pedro
P
D000907:Antibodies, Bacterial / Q000097:blood*; D016997:Coxiella burnetii / Q000276:immunology*; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011778:Q Fever / Q000175:diagnosis* / Q000453:epidemiology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M