[Gene transfection efficiency and destination of recombinant adenovirus gene transfer through internal jugular vein injection in rats].
OBJECTIVE
To investigate the adenovirus-mediated LacZ gene expression and the destination in different organs of SD rats after the intravenous injection in rats.


METHODS
Recombinant adenovirus vector containing LacZ was transferred to SD rats by injecting into the internal jugular vein. To identify the sites and periods of LacZ gene expression, X-gal staining was used to detect beta-gal level and period of LacZ gene expression of different organs in the transfected and non-transfected rats at different time intervals.


RESULTS
On the 1st day after the injection, the lung, liver, kidney, and spleen expressed some beta-gal; on the 3rd day after the injection, the lung, liver, kidney, and spleen expressed beta-gal obviously; their peak levels were on the 7th day; the beta-gal level decreased on the 14th day; beta-gal expression disappeared in the most organs except the lungs on the 28th day. In all animals, the brain did not express any beta-gal.


CONCLUSIONS
The adenovirus-mediated exogenous gene transfer in the internal jugular vein may be an effective approach of gene therapy in some diseases in the lung, liver, and kidney.
Ai
Yu-hang
YH
Intensive Care Unit, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhang
Li-na
LN
Gong
Hua
H
Peng
Liu
L
Zhang
Yang-de
YD
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D007275:Injections, Intravenous; D007601:Jugular Veins; D007763:Lac Operon / Q000235:genetics*; D008168:Lung / Q000378:metabolism; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection
D011994:Recombinant Proteins