title: Degradation of PrP(Sc) by keratinolytic protease from Nocardiopsis sp. TOA-1.
abstract: A keratinolytic alkaline proteae (NAPase) from Nocardiopsis sp. TOA-1 degraded a scrapie prion without any chemical or physical treatment. Optimal temperature and pH were 60 degrees C and above pH 10.0. The scrapie prion was completely degraded within 3 min under optimal conditions.
authors.affiliation: Department of Applied Chemistry and Biochemistry, Kyushu Sangyo University, Fukuoka, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Mitsuiki
authors.forename: Zhao
authors.initials: Z
authors.lastname: Hui
authors.forename: Daishi
authors.initials: D
authors.lastname: Matsumoto
authors.forename: Masashi
authors.initials: M
authors.lastname: Sakai
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Moriyama
authors.forename: Kensuke
authors.initials: K
authors.lastname: Furukawa
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kanouchi
authors.forename: Tatsuzo
authors.initials: T
authors.lastname: Oka
mesh_terms: D000192:Actinomycetales / Q000201:enzymology*; D000818:Animals; D001673:Biodegradation, Environmental; D006224:Cricetinae; D006863:Hydrogen-Ion Concentration; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism*; D018620:PrPSc Proteins / Q000378:metabolism*; D013696:Temperature
chemical_list: D018620:PrPSc Proteins; D010447:Peptide Hydrolases; C035855:keratinase