(-)Schisandrin B is more potent than its enantiomer in enhancing cellular glutathione and heat shock protein production as well as protecting against oxidant injury in H9c2 cardiomyocytes.
Effects of schisandrin B enantiomers ((+)Sch B and (-)Sch B) treatment on cellular reduced glutathione (GSH) level and heat shock protein (Hsp)25/70 production were investigated in H9c2 cardiomyocytes. (+)Sch B and (-)Sch B at 6.25 muM produced a time-dependent and biphasic change in cellular GSH level and Hsp25/70 production, with the stimulatory effect of (-)Sch B being more potent. The GSH- and Hsp-enhancing effects were accompanied by a parallel cytoprotection against xanthine oxidase/xanthine-induced toxicity, with the biphasic time course of (+)Sch B- or (-)Sch B-induced protection being superimposed with that of the increase in GSH level but not Hsp25/70 production. The results indicate that (-)Sch B produces more potent enhancing effects on cellular GSH and Hsp production as well as protection against oxidative injury than (+)Sch B in cardiomyocytes.
Chiu
Po Yee
PY
Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Leung
Hoi Yan
HY
Poon
Michel K T
MK
Mak
Duncan H F
DH
Ko
Kam Ming
KM
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D034242:Cyclooctanes / Q000737:chemistry / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D017705:Lignans / Q000737:chemistry* / Q000494:pharmacology*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016877:Oxidants / Q000378:metabolism*; D011083:Polycyclic Compounds / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D013237:Stereoisomerism; D013997:Time Factors; D019820:Xanthine / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
D034242:Cyclooctanes; D018840:HSP70 Heat-Shock Proteins; D017705:Lignans; D016877:Oxidants; D011083:Polycyclic Compounds; C015499:schizandrin B; D019820:Xanthine; D014969:Xanthine Oxidase; D005978:Glutathione