1,25-Dihydroxyvitamin D3 suppresses exportin expression in human promyelocytic leukemia HL-60 cells.
The active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3 (DVD), is a potent inducer of cell differentiation and inhibits proliferation of cells such as human promyelocytic leukemia HL-60 cells. In the present study, we examined the effects of DVD on the expression of exportin-1 and exportin-t, which play essential roles in the transport of mRNA and tRNA, respectively. The results of reverse transcription-polymerase chain reaction and quantitative real-time polymerase chain reaction indicated that DVD down-regulated the gene expression of these exportins. Western blotting revealed the decreased production of these proteins in DVD-treated cells. Thus, the present findings of decreased expression of exportin-1 and exportin-t induced by DVD can be correlated to inhibition of the proliferation of HL-60 cells.
Suzuki
Takuji
T
Laboratory of Cellular Biochemistry, Graduate School of Nutritional and Environmental Sciences, and COE for the 21th Century, University of Shizuoka, Japan.
Koyama
Yu
Y
Hayakawa
Sumio
S
Munakata
Hiroshi
H
Isemura
Mamoru
M
D049109:Cell Proliferation; D015973:Gene Expression Regulation, Leukemic; D018922:HL-60 Cells; D006801:Humans; D028884:Karyopherins / Q000235:genetics / Q000378:metabolism*; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000378:metabolism; D000097606:Exportin 1 Protein
D028884:Karyopherins; D029543:Nucleocytoplasmic Transport Proteins; D012333:RNA, Messenger; D012343:RNA, Transfer; D018160:Receptors, Cytoplasmic and Nuclear; D014807:Vitamin D; D000097606:Exportin 1 Protein; C503618:XPOT protein, human; C118756:dihydroxy-vitamin D3