UVA-mediated down-regulation of MMP-2 and MT1-MMP coincides with impaired angiogenic phenotype of human dermal endothelial cells.
UVA irradiation, dose-dependently (5-20 J/cm2), was shown to impair the morphogenic differentiation of human microvascular endothelial cells (HMECs) on Matrigel. Parallely, UVA down-regulated the expression of MMP-2 and MT1-MMP, both at the protein and the mRNA levels. On the contrary, the production of MMP-1 and TIMP-1 by HMECs increased following UVA treatment. The inhibitory effect of UVA on MMP expression and pseudotubes formation was mediated by UVA-generated singlet oxygen (1O2). The contribution of MT1-MMP, but not TIMP-1, to the regulation of HMECs' angiogenic phenotype following UVA irradiation was suggested using elastin-derived peptides and TIMP-1 blocking antibody, respectively.
Cauchard
Jean-Hubert
JH
CNRS UMR 6198, IFR 53 Biomolecules, Faculty of Medicine, 51, rue Cognacq Jay, Reims 51100, France.
Robinet
Arnaud
A
Poitevin
Stéphane
S
Bobichon
Hélene
H
Maziere
Jean-Claude
JC
Bellon
Georges
G
Hornebeck
William
W
D042501:Angiogenic Proteins / Q000235:genetics / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D015536:Down-Regulation / Q000528:radiation effects; D004549:Elastin / Q000494:pharmacology; D042783:Endothelial Cells / Q000378:metabolism / Q000528:radiation effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology / Q000528:radiation effects*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D053504:Matrix Metalloproteinases, Membrane-Associated; D010641:Phenotype; D026082:Singlet Oxygen / Q000494:pharmacology; D012867:Skin / Q000166:cytology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000494:pharmacology; D014466:Ultraviolet Rays
D042501:Angiogenic Proteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D026082:Singlet Oxygen; D004549:Elastin; D020782:Matrix Metalloproteinases; D053504:Matrix Metalloproteinases, Membrane-Associated; D020778:Matrix Metalloproteinase 2