Insulin vesicle release: walk, kiss, pause ... then run.
The mechanisms by which insulin-containing dense core secretory vesicles approach and finally fuse with the plasma membrane are of considerable current interest: defects in these processes may be one of the contributing factors to Type 2 diabetes. In this review, we discuss the molecular mechanisms involved in vesicle trafficking within the pancreatic beta-cell and the mechanisms whereby these may be regulated. We then go on to describe recent evidence that suggests that vesicle fusion at the plasma membrane is a partly reversible process ("kiss and run" or "cavity recapture"). We propose that vesicles may participate in a exo-endocytotic cycle in which a proportion of those that have already undergone an interaction with the plasma membrane may exchange exocytotic machinery with maturing vesicles.
Rutter
Guy A
GA
Department of Biochemistry, School of Medical Sciences, University Walk University of Bristol, Bristol, United Kingdom. g.a.rutter@bris.ac.uk
Hill
Elaine V
EV
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000503:physiopathology*; D004705:Endocytosis / Q000502:physiology; D005089:Exocytosis / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D022142:Secretory Vesicles / Q000378:metabolism*
D007328:Insulin