title: Multigenotype HCV-NS3 recombinant vaccinia viruses as a model for evaluation of cross-genotype immunity induced by HCV vaccines in the mouse.
abstract: Surrogate infections with HCV-recombinant vaccinia viruses (HCV-rVV) are a standard method to test the efficacy of hepatitis C virus (HCV) vaccine candidates in the mouse model. We established a panel of 16 HCV-rVV expressing the nonstructural protein 3 (NS3) of HCV genotypes 1a, 1b, 2, 3 and 4. Mice immunized with recombinant NS3 protein derived from HCV genotype 1b were challenged with the rVV. rVV-titers decreased up to 54-fold after subtype 1b challenge and up to 8.5-fold after subtype 1a challenge. No change was detected for genotype 2, 3, or 4. Our model is a convenient and reliable tool to analyze the induction of cross-genotype immunity by experimental vaccination of mice.
authors.affiliation: University of Heidelberg, Department of Gastroenterology, Hepatology, Infectious Diseases and Intoxications, Im Neuenheimer Feld 410, D-69120 Heidelberg, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Eisenbach
authors.forename: Anne
authors.initials: A
authors.lastname: Freyse
authors.forename: Catalin M
authors.initials: CM
authors.lastname: Lupu
authors.forename: Kilian
authors.initials: K
authors.lastname: Weigand
authors.forename: Evelyn
authors.initials: E
authors.lastname: Ernst
authors.forename: Birgit
authors.initials: B
authors.lastname: Hoyler
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Stremmel
authors.forename: Joachim J
authors.initials: JJ
authors.lastname: Bugert
authors.forename: Jens
authors.initials: J
authors.lastname: Encke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics*; D014761:Viral Hepatitis Vaccines / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases