Immunohistochemical study of immunoglobulin light chain amyloidosis with antibodies to the immunoglobulin light chain variable region.
To detect immunoglobulin (Ig) light chain amyloidosis (AL amyloidosis) in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry, polyclonal antibodies were generated against synthetic peptides corresponding to amino acids 1-19 of the Ig lambda light chain V lambda VI subgroup (anti-V lambda VI (1-19)) and the Ig kappa light chain Vkappa I subgroup (anti-Vkappa I (1-19)). Anti-V lambda VI (1-19) antibody reacted with amyloid deposits in 21 of 22 Alambda amyloidosis cases, and anti-Vkappa I (1-19) antibody reacted with amyloid deposits in 10 of 11 Akappa amyloidosis cases. Immunoreactivity varied in intensity by case and within specimens. Surprisingly, amyloid deposits were positive for anti-V kappa I (1-19) staining in one case of Alambda amyloidosis. Analysis of anti-V lambda VI (1-19) and anti-Vkappa I (1-19) antibody reactivity by ELISA showed some cross-reactivity with peptides other than antigen peptides. The antibodies were not reactive in all cases of AL amyloidosis examined but may be useful, together with anti-Ig constant region antibodies, for immunohistochemical diagnosis of AL amyloidosis.
Hoshii
Yoshinobu
Y
First Department of Pathology, Yamaguchi University School of Medicine, Ube, Japan. hoshii@yamaguchi-u.ac.jp
Kiyama
Makiko
M
Cui
Dan
D
Kawano
Hiroo
H
Ishihara
Tokuhiro
T
D000682:Amyloid / Q000378:metabolism; D000686:Amyloidosis / Q000276:immunology* / Q000473:pathology; D000906:Antibodies / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006331:Heart Diseases / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D007668:Kidney / Q000276:immunology / Q000473:pathology; D007674:Kidney Diseases / Q000276:immunology* / Q000473:pathology; D010446:Peptide Fragments / Q000276:immunology
D000682:Amyloid; D000906:Antibodies; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D010446:Peptide Fragments