title: Expression of the spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase (GAPDS) in rat testis.
abstract: The spermatogenic cell-specific variant of glyceraldehyde 3-phosphate dehydrogenase (GAPDS) has been cloned from a rat testis cDNA library and its pattern of expression determined. A 1,417 nucleotide cDNA has been found to encode an enzyme with substantial homology to mouse GAPDS (94% identity) and human GAPD2 (83% identity) isozymes. Northern blotting of rat tissue RNAs detected the 1.5 kb Gapds transcript in the testis and not in RNA from liver, spleen, epididymis, heart, skeletal muscle, brain, seminal vesicle, and kidney. The rat Gapds mRNA was first detected at day 29 of postnatal testis development, an age which coincides with the initial post-meiotic differentiation of round spermatids. When isolated rat spermatogenic cell RNA was probed for Gapds expression, transcripts were detected only in round spermatids and condensing spermatids, but not in pachytene spermatocytes, demonstrating haploid expression of the Gapds gene. However, immunohistochemical staining of rat testis sections with anti-GAPDS antisera did not detect GAPDS in round spermatids, but localized the protein only to stage XIII and later condensing spermatids as well as testicular spermatozoa, indicating that Gapds expression is translationally regulated. The current results are similar to those previously obtained for mouse GAPDS and human GAPD2, suggesting that reliable comparisons can be made between these species in toxicant screening and contraceptive development.
authors.affiliation: Gamete and Early Embryo Biology Branch, Reproductive Toxicology Division, National Health Effects and Environmental Research Laboratory, Office of Research and Development, US EPA, Research Triangle Park, North Carolina 27711, USA. welch.jeffrey@epa.gov
authors.forename: Jeffrey E
authors.initials: JE
authors.lastname: Welch
authors.forename: Randy R
authors.initials: RR
authors.lastname: Barbee
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Magyar
authors.forename: Donna O
authors.initials: DO
authors.lastname: Bunch
authors.forename: Deborah A
authors.initials: DA
authors.lastname: O'Brien
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment; D013094:Spermatozoa / Q000166:cytology / Q000201:enzymology; D013737:Testis / Q000166:cytology / Q000201:enzymology*; D014018:Tissue Distribution
chemical_list: D005987:Glyceraldehyde-3-Phosphate Dehydrogenases