title: [A cytological, immunophenotypical and cytogenetical study of 136 consecutive cases of B-cell chronic lymphoid hemopathies].
abstract: A cytological, immunophenotypical and cytogenetical study of 136 chronic B-cell proliferations (93 CLL, 43 B-cell lymphomas) was led in order to precise diagnosis and to characterize and appreciate chromosomal rearrangements. In this series, mainly selected on blood lymphocytosis criteria, B-CLL were twice more frequent than small B-cell lymphomas. Probes used revealed cryptic abnormalities, which remained unknown by conventional cytogenetics (CC). The frequency of clonal abnormalities (CC and FISH) was 74.8% for this series, with 74.4% for lymphomas and 75.3% for CLL, mainly of Binet stage A (69 A, 13 B, 1 C, 10 unspecified). Proportion was 88.4% in A stages and 84.6% in B stages. In CLL, 13q14 cryptic deletions and translocations were widely majority, 14q32 translocations and trisomy 12 being predominant in lymphoma series. Interphase FISH study of non-clonal metaphasic abnormalities with locus-specific probes often revealed unrecognised clones.
authors.affiliation: Laboratoire d'Hématologie, Service d'Hématologie Biologique, Pôle d'Oncohématologie, Hôpital de Hautepierre, avenue Molière, 67092 Strasbourg cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Struski
authors.forename: V
authors.initials: V
authors.lastname: Leymarie
authors.forename: C
authors.initials: C
authors.lastname: Helias
authors.forename: A
authors.initials: A
authors.lastname: Falkenrodt
authors.forename: C
authors.initials: C
authors.lastname: Fohrer
authors.forename: B
authors.initials: B
authors.lastname: Audhuy
authors.forename: B
authors.initials: B
authors.lastname: Lioure
authors.forename: P
authors.initials: P
authors.lastname: Moskovtchenko
authors.forename: I
authors.initials: I
authors.lastname: Mazurier
authors.forename: A-C
authors.initials: AC
authors.lastname: Galoisy
authors.forename: C
authors.initials: C
authors.lastname: Gervais
authors.forename: L
authors.initials: L
authors.lastname: Mauvieux
authors.forename: R
authors.initials: R
authors.lastname: Herbrecht
authors.forename: J-P
authors.initials: JP
authors.lastname: Bergerat
authors.forename: M
authors.initials: M
authors.lastname: Lessard
mesh_terms: D000782:Aneuploidy; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000235:genetics* / Q000648:ultrastructure; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics / Q000648:ultrastructure; D002999:Clone Cells / Q000473:pathology; D015331:Cohort Studies; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000235:genetics; D008297:Male; D009367:Neoplasm Staging; D017384:Sequence Deletion