Production of Fab fragment corresponding to surface protein antigen of Streptococcus mutans serotype c-derived peptide by Escherichia coli and cultured tobacco cells.
The cDNA of a mouse Fab fragment was cloned from a hybridoma cell line that produces a mouse monoclonal antibody, KH5, that reacts with the peptide fragment of the surface protein antigen of Streptococcus mutans serotype c (PAc). After transfection with cDNA, recombinant Fab fragments were produced by Escherichia coli (T15 Fab) and cultured tobacco cells (X253 and X262 Fabs). The antipeptide activities of T15 and X253 were similar to that of KH5. X253 was secreted into the culture media, which had a specific affinity for the PAc peptide.
Yano
Akira
A
Department of Oral Health, National Institute of Public Health, Saitama, Japan. akiray@ibrc.or.jp
Takekoshi
Masataka
M
Morita
Eiko
E
Imai
Susumu
S
Nisizawa
Tosiki
T
Hanada
Nobuhiro
N
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000737:chemistry; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism*; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D013295:Streptococcus mutans / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000378:metabolism*
D000954:Antigens, Surface; D007128:Immunoglobulin Fragments; D010455:Peptides