Screening and chromatographic assessing of a novel IgG biomimetic ligand.
A novel biomimetic ligand, N-benzyloxycarbonyl-l-tyrosine (N-cbz-l-Tyr), was screened by a combination method of molecular docking and immobilized receptor technique. Then, N-cbz-l-Tyr was immobilized on Sepharose CL-4B to prepare a specific affinity adsorbent for immunoglobulin G (IgG). Scatchard analysis of the binding isotherm for IgG on the adsorbent gave an association constant (K(a)) of 4.91 x 10(6) m(-1) and a theoretical maximum adsorption capacity of 17.3 mg IgG/mL gel. IgG with a purity of 98% was separated from human plasma by this new affinity adsorbent.
Feng
Hongqin
H
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116023, People's Republic of China.
Jia
Lingyun
L
Li
Honglin
H
Wang
Xicheng
X
D040761:Biomimetic Materials / Q000737:chemistry*; D032701:Biomimetics / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000302:isolation & purification*; D008024:Ligands; D008958:Models, Molecular; D018716:Molecular Mimicry; D015394:Molecular Structure; D015203:Reproducibility of Results; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
D007074:Immunoglobulin G; D008024:Ligands; C516280:N-benzyloxycarbonyl-l-tyrosine; D014443:Tyrosine; C035771:Sepharose CL 4B; D012685:Sepharose