Subcutaneous fat tissue for diagnosis and studies of systemic amyloidosis.
The systemic amyloidoses comprise a biochemically heterogeneous group of potentially lethal disorders. An early and precise diagnosis is crucial for the treatment and prognosis. Subcutaneous fat biopsy is a simple and safe method to obtain a diagnosis of systemic amyloidosis and the material can be used for exact determination of amyloid type. A method is described for immunochemical typing of the amyloid based on Western blot analysis combined with specific amyloid fibril protein antibodies.
Westermark
Per
P
Rudbeck Laboratory, Department of Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden. Per.Westermark@genpat.uu.se
Davey
Eva
E
Lindbom
Karolina
K
Enqvist
Stina
S
D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D000682:Amyloid / Q000032:analysis / Q000145:classification / Q000378:metabolism*; D000686:Amyloidosis / Q000145:classification / Q000175:diagnosis* / Q000378:metabolism; D001706:Biopsy; D015153:Blotting, Western; D004396:Coloring Agents; D003224:Congo Red; D006801:Humans; D007120:Immunochemistry / Q000379:methods; D040521:Subcutaneous Tissue / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*
D000682:Amyloid; D004396:Coloring Agents; D003224:Congo Red