Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines.
The concept of tumor stem cells (TSCs) provides a new paradigm for understanding tumor biology, although it remains unclear whether TSCs will prove to be a more robust model than traditional cancer cell lines. We demonstrate marked phenotypic and genotypic differences between primary human tumor-derived TSCs and their matched glioma cell lines. Unlike the matched, traditionally grown tumor cell lines, TSCs derived directly from primary glioblastomas harbor extensive similarities to normal neural stem cells and recapitulate the genotype, gene expression patterns, and in vivo biology of human glioblastomas. These findings suggest that TSCs may be a more reliable model than many commonly utilized cancer cell lines for understanding the biology of primary human tumors.
Lee
Jeongwu
J
Neuro-Oncology Branch, National Cancer Institute, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.
Kotliarova
Svetlana
S
Kotliarov
Yuri
Y
Li
Aiguo
A
Su
Qin
Q
Donin
Nicholas M
NM
Pastorino
Sandra
S
Purow
Benjamin W
BW
Christopher
Neil
N
Zhang
Wei
W
Park
John K
JK
Fine
Howard A
HA
D000818:Animals; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015894:Genome, Human / Q000235:genetics; D005838:Genotype; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D019656:Loss of Heterozygosity; D051379:Mice; D016513:Mice, SCID; D008954:Models, Biological; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000473:pathology*; D010641:Phenotype; D044967:Serum; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor