Identification and characterization of polymorphisms within the 5' flanking region, first exon and part of first intron of bovine GH gene.
The aim of the present study was to identify and characterize polymorphisms within the 5' flanking region, first exon and part of first intron of the bovine growth hormone gene among different beef cattle breeds: Nelore (n = 25), Simmental (n = 39), Simbrasil (n = 24), Simmental x Nelore (n = 30), Canchim x Nelore (n = 30) and Angus x Nelore (n = 30). Two DNA fragments (GH1, 464 bp and GH2, 453 bp) were amplified by polymerase chain reaction and then used for polymorphism identification by SSCP. Within the GH1 fragment, five polymorphisms were identified, corresponding to three different alleles: GH1.1, GH1.2 and GH1.3 (GenBank: AY662648, AY662649 and AY662650, respectively). These allele sequences were aligned and compared with bovine GH gene nucleotide sequence (GenBank: M57764 and AF118837), resulting in the identification of five insertion/deletions (INDELs) and five single nucleotide polymorphisms (SNPs). In the GH2 fragment two alleles were identified, GH2.1 and GH2.2 (GenBank: AY662651 and AY662652, respectively). The allele sequences were compared with GenBank sequences (M57764, AF007750 and AH009106) and three INDELs and four SNPs were identified. In conclusion, we were able to identify six new polymorphisms of the bovine GH gene (one INDEL and five SNPs), which can be used as molecular markers in genetic studies.
Ferraz
A L J
AL
Faculdade de Medicina Veterinária e Zootecnia, Departamento de Melhoramento e Nutrição Animal, FMVZ, UNESP, Botucatu, SP, Brazil. splinter_zoo2@yahoo.com
Bortolossi
J C
JC
Curi
R A
RA
Ferro
M I T
MI
Ferro
J A
JA
Furlan
L R
LR
D024506:5' Flanking Region / Q000235:genetics*; D000483:Alleles; D000818:Animals; D002417:Cattle / Q000145:classification / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D005091:Exons / Q000235:genetics; D005787:Gene Frequency; D023061:Gene Order; D005819:Genetic Markers / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D016415:Sequence Alignment / Q000662:veterinary
D017931:DNA Primers; D005819:Genetic Markers; C073294:growth hormone, bovine; D013006:Growth Hormone