Combined infrared multiphoton dissociation and electron-capture dissociation using co-linear and overlapping beams in Fourier transform ion cyclotron resonance mass spectrometry.
A novel set-up for Fourier transform ion cyclotron resonance mass spectrometry (FTICR) is reported for simultaneous infrared multiphoton dissociation (IRMPD) and electron-capture dissociation (ECD). An unmodified electron gun ensures complete, on-axis overlap between the electron and the photon beams. The instrumentation, design and implementation of this novel approach are described. In this configuration the IR beam is directed into the ICR cell using a pneumatically actuated mirror inserted into the ion-optical path. Concept validation was made using different combinations of IRMPD and ECD irradiation events on two standard peptides. The ability to perform efficient IRMPD, ECD and especially simultaneous IRMPD and ECD using lower irradiation times is demonstrated. The increase in primary sequence coverage, with the combined IRMPD and ECD set-up, also increases the confidence in peptide and protein assignments.
Mihalca
Romulus
R
FOM Institute for Atomic and Molecular Physics (AMOLF), Kruislaan 407, 1098 SJ Amsterdam, The Netherlands.
van der Burgt
Yuri E M
YE
McDonnell
Liam A
LA
Duursma
Marc
M
Cerjak
Iliya
I
Heck
Albert J R
AJ
Heeren
Ron M A
RM
D000595:Amino Acid Sequence; D004583:Electrons; D008555:Melitten / Q000737:chemistry; D008969:Molecular Sequence Data; D017785:Photons; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation* / Q000379:methods*; D013373:Substance P / Q000737:chemistry; D013997:Time Factors
D008555:Melitten; D013373:Substance P