Effects of 17beta-estradiol on the expression of IL-6, IL-11 and NF-kappaB in human MG-63 osteoblast-like cell line.
In order to characterize the effects of 17beta-estradiol (17beta-E2) on the expression of IL-6, IL-11 and NF-kappaB in the human MG-63 osteoblast-like cell line, the expression of IL-6 was detected by RT-PCR, Northern blot and Western blot. The expression of IL-11 was determined by RT-PCR, and NF-kappaB by Western blot. The results showed that 17beta-E2 down-regulated the expression of IL-6 mRNA and protein, IL-11 mRNA and NF-kappaB protein in MG-63 cells. It was suggested that the expression of NF-kappaB, IL-6 and IL-11 in MG-63 cells could be suppressed by 17beta-E2, and this might lend support to estrogen replacement therapy in postmenopausal women.
Wang
Yunlin
Y
Department of Gerontism, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Liao
Eyuan
E
Xiang
Guangda
G
Dai
Ruchun
R
Xiao
Xinhua
X
Luo
Xianghang
X
D045744:Cell Line, Tumor; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D017370:Interleukin-11 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000473:pathology*; D012516:Osteosarcoma / Q000473:pathology*
D017370:Interleukin-11; D015850:Interleukin-6; D016328:NF-kappa B; D004958:Estradiol