title: Indole-3-carbinol in the maternal diet provides chemoprotection for the fetus against transplacental carcinogenesis by the polycyclic aromatic hydrocarbon dibenzo[a,l]pyrene.
abstract: The fetus and neonate are sensitive targets for chemically induced carcinogenesis. Few studies have examined the risk/benefit of chemoprotective phytochemicals, given in the maternal diet, against transplacental carcinogenesis. In this study, B6129 SF1/J (AHR(b-1/d)) and 129Sv/ImJ (AHR(d/d)) mice were cross-bred. The polycyclic aromatic hydrocarbon, dibenzo[a,l]pyrene (DBP), was administered to pregnant mice (15 mg/kg, gavage) on gestation day 17, and 2000 p.p.m. indole-3-carbinol (I3C), a chemoprotective phytochemical from cruciferous vegetables, was fed to half of the mice from gestation day 9 until weaning. Offspring born to dams fed I3C exhibited markedly fewer mortalities (P < 0.0001). Maternal dietary exposure to I3C also significantly lowered lung tumor multiplicity (P = 0.035) in offspring surviving to 10 months of age. The I3C chemoprotection was independent of either maternal or fetal AHR genotype. The bioavailability of DBP to fetal target tissue was demonstrated by assessing DNA covalent adduction with a (33)P-post-labeling assay. The bioavailability of I3C was determined by dosing a subset of pregnant mice with [(14)C]-I3C. Addition of chemoprotective agents to the maternal diet during pregnancy and nursing may be an effective new approach in reducing the incidence of cancers in children and young adults.
authors.affiliation: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331-7301, USA.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yu
authors.forename: Brinda
authors.initials: B
authors.lastname: Mahadevan
authors.forename: Christiane V
authors.initials: CV
authors.lastname: Löhr
authors.forename: Kay A
authors.initials: KA
authors.lastname: Fischer
authors.forename: Mandy A
authors.initials: MA
authors.lastname: Louderback
authors.forename: Sharon K
authors.initials: SK
authors.lastname: Krueger
authors.forename: Clifford B
authors.initials: CB
authors.lastname: Pereira
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Albershardt
authors.forename: William M
authors.initials: WM
authors.lastname: Baird
authors.forename: George S
authors.initials: GS
authors.lastname: Bailey
authors.forename: David E
authors.initials: DE
authors.lastname: Williams
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage*; D001580:Benzopyrenes / Q000378:metabolism / Q000633:toxicity*; D001682:Biological Availability; D018736:DNA Adducts / Q000032:analysis; D004032:Diet; D005260:Female; D005315:Fetal Diseases / Q000139:chemically induced / Q000517:prevention & control*; D007211:Indoles / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000517:prevention & control; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000139:chemically induced / Q000517:prevention & control; D008223:Lymphoma / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D008431:Maternal-Fetal Exchange; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D011247:Pregnancy
chemical_list: D016588:Anticarcinogenic Agents; D001580:Benzopyrenes; D018736:DNA Adducts; D007211:Indoles; D016328:NF-kappa B; C016517:indole-3-carbinol; C041517:dibenzo(a,l)pyrene