Association of low density lipoprotein receptor related protein-associated protein (LRPAP1) gene insertion/deletion polymorphism with gallstone disease.
OBJECTIVE
Gallstones are byproducts of cholesterol supersaturated bile. Various studies have indicated that there might be a genetic predisposition to the disease. Receptor-associated protein (RAP) is a molecular chaperone for low density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD).


METHODS
To analyze the association of insertion/deletion polymorphism in GSD, 130 gallstone patients and 202 healthy subjects took part in the present study. For genotyping, polymerase chain reaction was followed by 2% agarose gel electrophoresis.


RESULTS
The results showed that frequencies of D and I allele were 65.77% and 34.23% in patients, 76.24% and 23.76% in controls, respectively. Frequency of I allele was significantly higher in the patient group than in the control group (P = 0.003).


CONCLUSIONS
In the present study I (insertion) allele was found to be associated with GSD.
Dixit
Manjusha
M
Department of Genetics, SGPGIMS, Lucknow, India.
Choudhuri
Gourdas
G
Keshri
Laxman Ji
LJ
Mittal
Balraj
B
D000328:Adult; D042882:Gallstones / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D020022:Genetic Predisposition to Disease; D006801:Humans; D007194:India / Q000453:epidemiology; D026563:LDL-Receptor Related Protein-Associated Protein / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic
D026563:LDL-Receptor Related Protein-Associated Protein