Inhibition of the alpha-mannosidase Man2c1 gene expression enhances adhesion of Jurkat cells.
Protein N-glycosylation plays very important roles in immunity and alpha-mannosidase is one of the key enzymes in N-glycosylation. This paper reports that inhibition of alpha-mannosidase Man2c1 gene expression enhances adhesion of Jurkat T cells. In comparison to the controls with normal expression of the enzyme, Jurkat cells with the inhibition of Man2c1 gene expression (AS cell) formed larger aggregates in culture, indicating an enhancement of adhesion between the cells. mRNA differential display analysis discovered up-regulation of several adhesion molecule genes in the AS cell. Because of the pivotal role played by CD54-LFA-1 interaction in immune cell interaction, this study focused on the contribution of enhanced expression of CD54 and LFA-1 to the enhanced adhesion of AS Jurkat cells. These facts, including increased binding of AS cells to ICAM-1-Fc, Mg(2+) activation of the binding of AS cells to ICAM-1-Fc and enhanced aggregation of AS cells, together with the inhibiting effect of a blocking CD11a mAb on the binding to ICAM-1-Fc and aggregation of the cells demonstrate an important contribution of enhanced CD54-LFA-1 interaction to increased adhesion between AS cells. The enhanced CD54-LFA-1 interaction also resulted in increased adhesion between AS Jurkat T cells and Raji B cells. In addition, AS cells showed cytoskeletal rearrangement. The data imply a biological significance of MAN2C1 in T-cell functioning.
Qu
Li
L
Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.
Ju
Ji Yu
JY
Chen
Shuang Ling
SL
Shi
Yan
Y
Xiang
Zhi Guang
ZG
Zhou
Yi Qun
YQ
Tian
Yun
Y
Liu
Yin
Y
Zhu
Li Ping
LP
D002448:Cell Adhesion / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D019169:Jurkat Cells / Q000276:immunology / Q000502:physiology*; D008361:Mannosidases / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D043323:alpha-Mannosidase
D018799:Intercellular Adhesion Molecule-1; D008361:Mannosidases; C511245:MAN2C1 protein, human; D043323:alpha-Mannosidase