[A method based on image processing and analyzing technology for estimating the activity of mesenchymal stem cells].
Cell culture is one of the usual methods for studying living cell and tissue, the method presented in this paper is based on image processing and analyzing technology for activity estimation of mesenchymal stem cells (MSCs). The existing activity estimation methods are costly, complex and invasive. In this method, thresholding is used to preprocess image and to separate out the growth hallow. Then the area is calculated by counting the pixels of the growth hallow. The changes of the activity estimated by this method are similar to those by corresponding cellular experiments. Compared with the existing methods in biology, medicine or medical cellular science, this method is easier, faster, cost-effective and non-invasive. The proposed method has been proved to be efficient by primary experiments of MSCs.
Xie
Lizhe
L
Biomedical Engineering Center, Box 373, Sichuan University, Chengdu 60065, China.
Wu
Jiang
J
Li
Deyu
D
Lin
Jiangli
J
Wang
Tianfu
T
Zheng
Changqiong
C
D000818:Animals; D002478:Cells, Cultured; D005269:Femur / Q000166:cytology; D007091:Image Processing, Computer-Assisted / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley