[In vitro modification of antioxidant response and its influence on cytokine synthesis in children with renal failure].
Recently it was shown that oxidative stress has a negative influence on immunological response. The aim of the study was to investigate the influence of N-acetylcysteine on intracellular oxidative stress and cytokine synthesis in peripheral blood lymphocytes in children with end stage renal disease (ESRD). MATERIAL AND METHODS. We examined 25 children (age 2-16 ys.) with ESRD. To determine oxidative stress we used dihydrorhodamine 123 (DHR). Intracellular oxidation of DHR in T. lymphocytes reflected intracellular oxidative stress within cells. The intracellular synthesis of cytokines (IL-2, IFN-r, IL-4, IL-6) was detected with flow cytometty RESULTS. We found lower intracellular stress and higher cytokines synthesis after incubation with N-acetylcysteine (NA C) in all subpopulations of lymphocytes. CONCLUSIONS. Our results shows that patients with ESRD present increased oxidative stress in T lymphocytes which may lead to decreased cytokines synthesis and abnormal immune response. NAC has favorable influence on these abnormalities.
Jacek
Zachwieja
Z
Akademia Medyczna w Poznaniu, Pracownia Dializ, Klinika Kardiologii i Nefrologii Dzieciecej. J.Zachwieja@mp.pl
Marcin
Zaniew
Z
Maria
Lewandowska-Stachowiak
LS
Ewa
Stefaniak
S
Alfred
Warzywoda
W
Aldona
Siwińska
S
D000293:Adolescent; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use*; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000096:biosynthesis* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007676:Kidney Failure, Chronic / Q000188:drug therapy*; D008297:Male
D000975:Antioxidants; D016207:Cytokines; D007376:Interleukin-2; D015850:Interleukin-6; D015847:Interleukin-4; D007371:Interferon-gamma