Identification of a novel antigen of Schistosoma mansoni shared with Plasmodium falciparum and evaluation of different cross-reactive antibody subclasses induced by human schistosomiasis and malaria.
Plasmodium falciparum and Schistosoma mansoni are often found in human coinfections, and cross-reactive antibodies to different components of the two parasites have been detected. In this work, we identified a cross-reactive S. mansoni gene product, referred to as SmLRR, that seems to belong to the leucine-rich repeat protein family. Comparative analysis of SmLRR revealed 57% similarity with a putative gene product encoded in the P. falciparum genome. Antibodies to SmLRR were found in experimental infections and in both S. mansoni- and P. falciparum-infected individuals. Correlative analysis of human anti-SmLRR responses in Kenya and Uganda suggested that malaria and schistosomiasis drive the immunoglobulin G3 (IgG3) and IgG4 isotypes, respectively, against SmLRR, suggesting that there is differential regulation of cross-reactive isotypes depending on the infection. In addition, the levels of anti-SmLRR IgG4, but not the levels of IgG3, correlated positively with the intensity of S. mansoni infection.
Pierrot
Christine
C
Unité Inserm 547, IFR 17, Institut Pasteur de Lille, 1 rue du Prof. Calmette, 59019 Lille, France.
Wilson
Shona
S
Lallet
Hélène
H
Lafitte
Sophia
S
Jones
Frances M
FM
Daher
Wassim
W
Capron
Monique
M
Dunne
David W
DW
Khalife
Jamal
J
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000913:Antibodies, Protozoan / Q000097:blood*; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification; D016778:Malaria, Falciparum / Q000276:immunology*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*
D000909:Antibodies, Helminth; D000913:Antibodies, Protozoan; D000947:Antigens, Helminth; D007074:Immunoglobulin G