Gene therapy of the ischemic lower limb--Therapeutic angiogenesis.
The limitations of surgical revascularisation and pharmacological treatment in peripheral arterial occlusive disease (PAOD) are well recognized. Therapeutic options for critical leg ischemia are consequently limited to percutaneous transluminal angioplasty (PTA) or surgical revascularisation. Unfortunately, many patients with critical leg ischemia are poor candidates for either procedure. Therapeutic angiogenesis is a novel promising tool to treat these patients. Experimental and clinical and trials of gene transfer for therapeutic angiogenesis have already shown some clinical efficacy. This review is focused on gene transfer techniques in preclinical and clinical therapeutic angiogenesis, angiogenic growth factors, vectors, delivery methods and routes. The results of clinical and experimental studies, safety and side effects of gene therapy, and the perspectives of future research are also discussed.
Bobek
Vladimir
V
Third Faculty of Medicine, Charles University Prague, Department of Tumor Biology, Czech Republic. vbobek@centrum.cz
Taltynov
Oliver
O
Pinterova
Daniela
D
Kolostova
Katarina
K
D000818:Animals; D001157:Arterial Occlusive Diseases / Q000378:metabolism / Q000628:therapy*; D002986:Clinical Trials as Topic; D042783:Endothelial Cells / Q000378:metabolism / Q000637:transplantation; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000639:trends*; D005822:Genetic Vectors; D006801:Humans; D007511:Ischemia / Q000378:metabolism / Q000628:therapy*; D035002:Lower Extremity / Q000098:blood supply*; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D042442:Vascular Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism
D042442:Vascular Endothelial Growth Factors