title: Use of alum and inactive Bordetella pertussis for generation of antibodies against synthetic peptides in mice.
abstract: We have investigated the efficacy of the combined use of Alum and inactive Bordetella pertussis (iBP) adjuvants for eliciting anti-peptide antibodies. ICR mice were immunized four times at 3-week intervals with each of 7 free (i.e., not conjugated to any carrier) synthetic peptides of 15-17 amino acid residues in Alum + iBP, in the commonly used adjuvant protocols (CFA; CFA (initial) followed by IFA), or in CFA + iBP. Serum samples after 3 and 4 injections were tested by RIA. Use of Alum + iBP greatly increased the production of antibodies for most of the peptides. The results have important implications for human vaccine formulation involving peptides.
authors.affiliation: Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Maruta
authors.forename: Minako
authors.initials: M
authors.lastname: Oshima
authors.forename: Philip R
authors.initials: PR
authors.lastname: Deitiker
authors.forename: Maki
authors.initials: M
authors.lastname: Ohtani
authors.forename: M Zouhair
authors.initials: MZ
authors.lastname: Atassi
mesh_terms: D000536:Aluminum Hydroxide / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology*; D000917:Antibody Formation / Q000276:immunology; D001886:Bordetella pertussis / Q000276:immunology*; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology; D008055:Lipids / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D011863:Radioimmunoassay
chemical_list: D000906:Antibodies; D008055:Lipids; D010455:Peptides; C114843:incomplete Freund's adjuvant; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant