title: A genetic linkage map of Silene vulgaris based on AFLP markers.
abstract: A genetic linkage map of an intraspecific cross between 2 Silene vulgaris s.l. ecotypes is presented. Three-hundred AFLP markers from 2 different restriction enzyme combinations were used to genotype an F2 mapping population. Maternal and paternal pure-coupling phase maps with 114 and 186 markers on 12 and 13 linkage groups, respectively, were constructed. Total map length of the paternal and maternal maps are 547 and 446 Kosambi cM, respectively. Nearly half of the markers (49%) exhibited significant transmission ratio distortion. Genome coverage and potential causes of the observed segregation ratio distortions are discussed. The maps represent a first step towards the identification of quantitative trait loci associated with habitat adaptation in the non-model species Silene vulgaris.
authors.affiliation: ETH Zürich, Institute of Integrative Biology, Plan Ecological Genetics, 8092 Zürich, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Bratteler
authors.forename: Christian
authors.initials: C
authors.lastname: Lexer
authors.forename: Alex
authors.initials: A
authors.lastname: Widmer
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics; D017632:Asbestos, Serpentine / Q000009:adverse effects; D020090:Chromosome Segregation / Q000187:drug effects; D003433:Crosses, Genetic; D016172:DNA Fingerprinting / Q000379:methods; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D005838:Genotype; D005810:Multigene Family; D040641:Quantitative Trait Loci / Q000235:genetics; D029751:Silene / Q000235:genetics*
chemical_list: D017632:Asbestos, Serpentine; D005819:Genetic Markers