Determination of the big head carp myofibrillar (Aristichthys nobilis) adenosine triphosphatase activity by ion chromatography.
A method for the determination of adenosine triphosphatase (ATPase) activity of myofibrils of big head carp by using ion chromatography was introduced. Adenosine triphosphate (ATP), adenosine diphosphate (ADP) and orthophosphate (Pi) were separated completely. Recoveries for ATP, ADP and Pi were 98+/-5%, 97+/-4% and 98+/-5%, respectively. Pi liberated from ATP during reaction was monitored by ion chromatography using the suggested method. This method was applicable to the determination of myofibrils ATPase activity for quick quality evaluation of surimi.
Gao
Rui-chang
RC
College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China.
Xue
Chang-hu
CH
Yuan
Li
L
Li
Zhao-jie
ZJ
Xue
Yong
Y
Cui
Fengxia
F
Sun
Yan
Y
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002138:Calibration; D002347:Carps; D002853:Chromatography, Liquid / Q000379:methods*; D006868:Hydrolysis; D015203:Reproducibility of Results
D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases