Characterization of two populations of mesenchymal progenitor cells in umbilical cord blood.
Umbilical cord blood (UCB) is a valuable source for hematopoietic progenitor cell therapy. Moreover, it contains another subset of non-hematopoietic population referred to as mesenchymal progenitor cells (MPCs), which can be ex vivo expanded and differentiated into osteoblasts, chondrocytes and adipocytes. In this study, we successfully isolated the clonogenic MPCs from UCB by limiting dilution method. These cells exhibited two different morphologic phenotypes, including flattened fibroblasts (majority) and spindle-shaped fibroblasts (minority). Both types of MPCs shared similar cell surface markers except CD90 and had similar osteogenic and chondrogenic potentials. However, the spindle-shaped clones possessed the positive CD90 expression and showed a greater tendency in adipogenesis, while the flattened clones were CD90 negative cells and showed a lower tendency in adipogenesis. The high number of flattened MPCs might be linked to the less sensitivity of UCB-derived MPCs in adipogenic differentiation.
Chang
Yu-Jen
YJ
Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan.
Tseng
Ching-Ping
CP
Hsu
Lee-Feng
LF
Hsieh
Tzu-Bou
TB
Hwang
Shiaw-Min
SM
D050156:Adipogenesis; D002454:Cell Differentiation; D002469:Cell Separation; D048430:Cell Shape; D020219:Chondrogenesis; D002999:Clone Cells / Q000166:cytology; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis; D018800:Thy-1 Antigens / Q000032:analysis
D008565:Membrane Proteins; D018800:Thy-1 Antigens