Fluorometric study on the interaction between lomefloxacin and bovine lactoferrin.
The binding of lomefloxacin to bovine lactoferrin (BLf) in a dilute aqueous solution was studied using fluorescence spectra. The binding constant (K) and the number of binding sites (n) were obtained by a fluorescence quenching method. The binding distance (r) and energy-transfer efficiency (E) between lomefloxacin and bovine lactoferrin were also obtained according to the mechanism of Foörster-type dipole-dipole nonradiative energy-transfer. The effect of lomefloxacin on the conformation of bovine lactoferrin was also analyzed by synchronous fluorescence spectroscopy. The interaction between lomefloxacin and bovine lactoferrin is strong. Lomefloxacin can affect the conformation of bovine lactoferrin to some degree.
Chen
Xiang
X
Department of Chemistry, Zhejiang University, Hangzhou, China.
Fan
Ji-Cai
JC
Wang
Yun
Y
Fan
Cheng-Ping
CP
Shang
Zhi-Cai
ZC
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004735:Energy Transfer; D024841:Fluoroquinolones / Q000737:chemistry* / Q000378:metabolism; D007781:Lactoferrin / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D013050:Spectrometry, Fluorescence / Q000379:methods*
D024841:Fluoroquinolones; D007781:Lactoferrin; C053091:lomefloxacin