An improved electron microscopic technique for the immunolabeling of Cryptosporidium parvum oocysts.
A technique was developed for immunolabeling Cryptosporidium parvum oocysts for subsequent observation by transmission electron microscopy. This method was developed to maintain architectural integrity of the oocyst wall and improve fixation of internal contents. The improved fixation and embedding method permits efficient immunolabeling of both nonexcysted and excysted C. parvum oocysts and may be applicable to other oocyst- and cyst-forming protozoa.
Jenkins
Mark C
MC
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA. mjenkins@anri.barc.usda.gov
Murphy
Charles
C
Trout
James
J
Fayer
Ronald
R
D000818:Animals; D002417:Cattle; D016785:Cryptosporidium parvum / Q000648:ultrastructure*; D005404:Fixatives; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D033661:Oocysts / Q000648:ultrastructure; D016613:Plastic Embedding / Q000379:methods
D005404:Fixatives