An alternative spliced transcript of ADAMTS4 is present in human synovium from OA patients.
The major proteoglycan of articular cartilage aggrecan is a substrate for ADAMTS4. RT-PCR analysis of human osteoarthritic (OA) synovial co-cultures using oligonucleotide primers designed to amplify across the exon 8/9 junction of human ADAMTS4 resulted in the amplification of two products, the expected product and a smaller product missing 161 bp from the 5' end of exon 9, the result of alternative splicing in which exon 8 joins to a cryptic 3' splice site within exon 9. The protein produced would be identical to human ADAMTS4 up to Arg(696), and would have a new C-terminal domain with no commonality with the ADAMTS4 spacer domain. Changes in the C-terminal domain of ADAMTS4 may alter its substrate specificity.
Wainwright
Shane D
SD
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, Wales, CF10 3US, United Kingdom.
Bondeson
Jan
J
Hughes
Clare E
CE
D051722:ADAM Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000071121:ADAMTS4 Protein; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010003:Osteoarthritis / Q000201:enzymology* / Q000235:genetics*; D011348:Procollagen N-Endopeptidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013583:Synovial Membrane / Q000201:enzymology*
D018076:DNA, Complementary; D012333:RNA, Messenger; D051722:ADAM Proteins; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein; C000603918:ADAMTS4 protein, human