title: Simultaneous detection of tau phospho-epitopes with haptenylated antibodies.
abstract: Neurofibrillary tangles as a neuropathological hallmark of Alzheimer's disease are mainly composed of abnormally phosphorylated microtubule-associated protein tau. The present work was primarily focused on the immunohistochemical characterization of recently developed monoclonal antibodies directed against disease-associated epitopes. Anti-phospho-threonine 212/phospho-serine 214 (HPT-1), anti-phospho-threonine 231/phospho-serine 235 (HPT-101) and their biotinylated derivatives were shown to be sensitive markers for the immunohistochemical detection of neuropathological alterations during Alzheimer's disease. Triple carbocyanine immunofluorescence labelling was based on digoxigenylated, fluoresceinated and biotinylated primary antibodies. AT8-immunolabelling of phospho-serine 202 and phospho-threonine 205 combined with HPT-1 and HPT-101-staining revealed similar distribution patterns of the three double-phosphorylated tau epitopes in the neocortex of patients with Alzheimer's disease.
authors.affiliation: Paul Flechsig Institute for Brain Research, University of Leipzig, Germany. hartig@medizin.uni-leipzig.de
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Härtig
authors.forename: Jörg
authors.initials: J
authors.lastname: Lehmann
authors.forename: Jens
authors.initials: J
authors.lastname: Stieler
authors.forename: David
authors.initials: D
authors.lastname: Singer
authors.forename: Jens
authors.initials: J
authors.lastname: Grosche
authors.forename: Thomas
authors.initials: T
authors.lastname: Arendt
authors.forename: Ralf
authors.initials: R
authors.lastname: Hoffmann
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000939:Epitopes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D019579:Neocortex / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D016875:tau Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; C054369:MAPT protein, human; D009419:Nerve Tissue Proteins; D016875:tau Proteins; D013912:Threonine; D012694:Serine