Tebufenozide resistance selected in Plutella xylostella and its cross-resistance and fitness cost.
A susceptible strain of diamondback moth, Plutella xylostella (L.), was used to select for resistance to tebufenozide in the laboratory. After continuous selection with tebufenozide 17 times during 35 generations, a resistant strain was achieved with high resistance to tebufenozide (RR 93.8). Bioassay revealed that this strain showed high cross-resistance to abamectin (RR 35.7), methoxyfenozide (29.1) and JS118 (16.5), and a little to deltamethrin (3.9), but no obvious cross-resistance was found to cypermethrin (1.3), fipronil (1.3), trichlorfon (1.1), chlorfenapyr (1.0), phoxim (0.9) and acephate (0.8). The resistant and susceptible insects had similar development rates, but life table tests indicated that the resistant strain showed reproductive disadvantages, including decreased copulation rate, reproductivity and hatchability. When compared with the susceptible strain, the resistant insects had a relative fitness of only 0.3. This indicated that tebufenozide resistance selected under laboratory conditions had considerable fitness costs in this pest, and therefore rotational use of insecticides without cross-resistance is recommended to delay development of resistance.
Cao
Guangchun
G
Key Laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Han
Zhaojun
Z
D000818:Animals; D005260:Female; D006834:Hydrazines; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides; D008018:Life Cycle Stages; D008297:Male; D009036:Moths / Q000235:genetics*; D012098:Reproduction; D012641:Selection, Genetic
D006834:Hydrazines; D007306:Insecticides; C082026:tebufenozide