title: [Relationship of trichloroethylene-induced medicamentosa like dermatitis to HLA-DMA and HLA-DMB].
abstract: OBJECTIVE
  To investigate the susceptibility of trichloroethylene-induced medicamentosa like dermatitis by comparing the frequency of HLA-DMA and HLA-DMB in patients with trichloroethylene-induced medicamentosa like dermatitis and in normal controls.
  
  
  METHODS
  The DNA of lymphocytes in 61 patients with trichloroethylene-induced medicamentosa like dermatitis and in 60 people as the normal control were abstracted by using touchdown PCR amplification of HLA-DMA and HLA-DMB. Then through restriction fragment length polymorphism (RFLP) and sequence base typing, the alleles and genotypes were confirmed. The frequency of HLA-DMA and HLA-DMB in the two groups was compared.
  
  
  RESULTS
  The HLA-DMA*0101 allele frequency in patients with trichloroethylene-induced medicamentosa like dermatitis was significantly higher than in the control group (71.3% vs 55.0%, P < 0.05). The allele frequency of HLA-DMA*0103 was significantly higher in the patient group than in the control group (11.5% vs 3.3%, P < 0.05). The ratio of *0102 homozygotes of HLA-DMA*0102 in the patient group was significantly higher than in the control group (25.0% vs 8.2%, P < 0.05). The ratio of *0102 heterozygotes of HLA-DMB*0101 in the patient group was lower than in the control group (P < 0.05).
  
  
  CONCLUSIONS
  The polymorphisms of DMA may be related to the susceptibility of the patients with trichloroethylene-induced medicamentosa like dermatitis.
authors.affiliation: Department of Environmental Hygiene, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yue
authors.forename: Han-lin
authors.initials: HL
authors.lastname: Huang
authors.forename: Zhen-lie
authors.initials: ZL
authors.lastname: Huang
authors.forename: Lai-yu
authors.initials: LY
authors.lastname: Li
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Liang
authors.forename: Xue-min
authors.initials: XM
authors.lastname: Chen
mesh_terms: D000483:Alleles; D009783:Dermatitis, Occupational / Q000209:etiology / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006681:HLA-D Antigens / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014241:Trichloroethylene / Q000009:adverse effects*
chemical_list: D006681:HLA-D Antigens; C084245:HLA-DM antigens; D014241:Trichloroethylene