Comparison of an indirect format ELISA on modified graphite and polystyrene surfaces against triazines.
An indirect format enzyme-linked immuno-sorbent assay (ELISA) on graphite rods ( 0.8 mm x 20 mm) and, for comparison, on microtiter plates has been developed against terbuthylazine. For this purpose, a series of 2-aminoalkyl-4-chloro-6-terbuthyl-s-triazine-2,6-diamine ELISA haptens with alkyl spacer lengths of 2, 4, 6, and 8 CH2 groups has been synthesized. The graphite rods or the microtiter plates were covered by a polymerized glutaraldehyde network, and the ELISA haptens have been coupled by imino coupling to the free aldehyde groups of that network. epsilon-Aminocaproic acid has been used as an agent to block unspecific binding sites. The ELISA was run in a competitive mode, where the immobilized ELISA hapten and the solute terbuthylazine as a target analyte compete for the solute antibody.
Fouad
Dina
D
Chemistry Department, Faculty of Science, Assiut University, Egypt.
Sound
Iris
I
Holthues
Heike
H
Pfeifer-Fukumura
Ursula
U
Hammam
Abbas M
AM
Ibrahim
Said A
SA
Baumann
Wolfram
W
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006108:Graphite; D006241:Haptens; D011137:Polystyrenes; D013499:Surface Properties; D014227:Triazines / Q000032:analysis*
D006241:Haptens; D011137:Polystyrenes; D014227:Triazines; D006108:Graphite