Estrogen-dependent synthesis and release of a protein (CUPED) by cultured uterine explants from the domestic cat.
Media from cultured cat endometrial explants were analyzed for the presence of a previously characterized high molecular weight estrogen-dependent secretory protein (CUPED) by polyacrylamide gel electrophoresis and radioimmunoassay (RIA). Both L-[(3)H]-leucine and D-[(3)H]-glucosamine were incorporated into newly synthesized CUPED during the culture of endometrial explants obtained from estradiol-treated ovariectomized cats, but not during the culture of tissue obtained from untreated ovariectomized animals or estradiol-primed ovariectomized animals treated with progesterone. The addition of tunicamycin to the culture medium inhibited the synthesis and release of the glycosylated form of CUPED from endometrial explants obtained from estradiol-treated cats. These data demonstrate that CUPED is synthesized and released in vitro from endometrial explants obtained from estradiol-treated cats as a glycoprotein possessing N-linked oligosaccharide chains.
Verhage
H G
HG
Department of Obstetrics and Gynecology, University of Illinois College of Medicine at Chicago, Chicago, IL 60680 USA; Department of Physiology and Biophysics (RCJ), University of Illinois College of Medicine at Chicago, Chicago, IL 60680 USA.
Fazleabas
A T
AT
Jaffe
R C
RC
Murray
M K
MK