Tal1/Scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) embryonic stem cells.
The development of embryonic stem cell (ESC) therapies requires the establishment of efficient methods to differentiate ESCs into specific cell lineages. Here, we report the in vitro differentiation of common marmoset (CM) (Callithrix jacchus) ESCs into hematopoietic cells after exogenous gene transfer using vesicular stomatitis virus-glycoprotein-pseudotyped lentiviral vectors. We transduced hematopoietic genes, including tal1/scl, gata1, gata2, hoxB4, and lhx2, into CM ESCs. By immunochemical and morphological analyses, we demonstrated that overexpression of tal1/scl, but not the remaining genes, dramatically increased hematopoiesis of CM ESCs, resulting in multiple blood-cell lineages. Furthermore, flow cytometric analysis demonstrated that CD34, a hematopoietic stem/progenitor cell marker, was highly expressed in tal1/scl-overexpressing embryoid body cells. Similar results were obtained from three independent CM ESC lines. These results suggest that transduction of exogenous tal1/scl cDNA into ESCs is a promising method to induce the efficient differentiation of CM ESCs into hematopoietic stem/progenitor cells.
Kurita
Ryo
R
Department of Molecular Genetics, Division of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Fukuoka 812-8582, Japan.
Sasaki
Erika
E
Yokoo
Tomoko
T
Hiroyama
Takashi
T
Takasugi
Kashiya
K
Imoto
Hideyuki
H
Izawa
Kiyoko
K
Dong
Yan
Y
Hashiguchi
Takao
T
Soda
Yasushi
Y
Maeda
Toyoki
T
Suehiro
Youko
Y
Tanioka
Yoshikuni
Y
Nakazaki
Yukoh
Y
Tani
Kenzaburo
K
D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology; D002144:Callithrix; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D004622:Embryo, Mammalian / Q000166:cytology*; D015870:Gene Expression / Q000187:drug effects; D005822:Genetic Vectors / Q000235:genetics; D006133:Growth Substances / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects; D014161:Transduction, Genetic
D018952:Antigens, CD34; D006133:Growth Substances; D011518:Proto-Oncogene Proteins