title: Characterization of the porcine acyl-CoA synthetase long-chain 4 gene and its association with growth and meat quality traits.
abstract: Summary Long-chain acyl-CoA synthetase (ACSL) catalyses the formation of long-chain acyl-CoA from fatty acid, ATP and CoA, activating fatty acids for subsequent reactions. Long-chain acyl-CoA synthetase thus plays an essential role in both lipid biosynthesis and fatty acid degradation. The ACSL4 gene was evaluated as a positional candidate gene for the quantitative trait loci (QTL) located between SW2456 and SW1943 on chromosome X. We have sequenced 4906 bp of the pig ACSL4 mRNA. Sequence analysis allowed us to identify 10 polymorphisms located in the 3'-UTR region and to elucidate two ACSL4 haplotypes. Furthermore, a QTL and an association study between polymorphisms of the ACSL4 gene and traits of interest were carried out in an Iberian x Landrace cross. We report QTL that have not been previously identified, and we describe an association of the ACSL4 polymorphisms with growth and percentage of oleic fatty acid. Finally, we have determined allelic frequencies in 140 pigs belonging to the Iberian, Landrace, Large White, Meishan, Pietrain, Duroc, Vietnamese, Peccary and Babirusa populations.
authors.affiliation: Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain. anna.mercader@uab.es
authors.forename: A
authors.initials: A
authors.lastname: Mercadé
authors.forename: J
authors.initials: J
authors.lastname: Estellé
authors.forename: M
authors.initials: M
authors.lastname: Pérez-Enciso
authors.forename: L
authors.initials: L
authors.lastname: Varona
authors.forename: L
authors.initials: L
authors.lastname: Silió
authors.forename: J L
authors.initials: JL
authors.lastname: Noguera
authors.forename: A
authors.initials: A
authors.lastname: Sánchez
authors.forename: J M
authors.initials: JM
authors.lastname: Folch
mesh_terms: D000818:Animals; D003066:Coenzyme A Ligases / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes; D008460:Meat; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000032:analysis; D017423:Sequence Analysis, RNA; D013552:Swine / Q000235:genetics* / Q000254:growth & development
chemical_list: D012333:RNA, Messenger; D003066:Coenzyme A Ligases