Proteomics-based expression library screening (PELS): a novel method for rapidly defining microbial immunoproteomes.
Current methodologies for global identification of microbial proteins that elicit host humoral immune responses have several limitations and are not ideally suited for use in the postgenomic era. Here we describe a novel application of proteomics, proteomics-based expression library screening, to rapidly define microbial immunoproteomes. Proteomics-based expression library screening is broadly applicable to any cultivable, sequenced pathogen eliciting host antibody responses and hence is ideal for rapidly mining microbial proteomes for targets with diagnostic, prophylactic, and therapeutic potential. In this report, we demonstrate "proof-of-principle" by identifying 207 proteins of the Escherichia coli O157:H7 immunome in bovine reservoirs in only 3 weeks.
Kudva
Indira T
IT
Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
Krastins
Bryan
B
Sheng
Haiqing
H
Griffin
Robert W
RW
Sarracino
David A
DA
Tarr
Phillip I
PI
Hovde
Carolyn J
CJ
Calderwood
Stephen B
SB
John
Manohar
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D019453:Escherichia coli O157 / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000276:immunology*; D015723:Gene Library; D013058:Mass Spectrometry; D020543:Proteome / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D029968:Escherichia coli Proteins; D020543:Proteome; D011994:Recombinant Proteins