Methodological approaches to exploring epileptic disorders in the human brain in vitro.
Brain surgery, and in particular epilepsy surgery, offers the unique opportunity to study viable human central nervous tissue in vitro. This does not only open a window to address the basic mechanisms underlying human disease, such as epilepsy, but it allows to venture into investigating neurophysiological functions per se. In the present paper, we describe the most commonly used methods in the electrophysiological (and, at least to some extent, also histochemical and molecular) analysis of human tissue in vitro. In addition, we consider the pitfalls and limitations of such studies, in particular regarding the issue of tissue sampling procedures and control experiments.
Köhling
Rüdiger
R
Institute of Physiology, University of Rostock, 18055 Rostock, Germany.
Avoli
Massimo
M
D001706:Biopsy / Q000379:methods / Q000592:standards; D001921:Brain / Q000378:metabolism / Q000503:physiopathology*; D004210:Dissection / Q000379:methods; D004594:Electrophysiology / Q000379:methods*; D004827:Epilepsy / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D009448:Neurochemistry / Q000379:methods*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009480:Neuropharmacology / Q000379:methods; D009924:Organ Culture Techniques / Q000379:methods; D018408:Patch-Clamp Techniques / Q000379:methods