title: Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells: in vitro analysis.
abstract: OBJECTIVE
  To analyze, in vitro, the effects of acetylsalicylic acid (aspirin) and acetic acid solutions on VX2 carcinoma cells in suspension and to examine the correlation between these effects and neoplastic cell death.
  
  
  METHODS
  The VX2 tumor cells (10(7) cells/ml) were incubated in solutions containing differing concentrations (2.5% and 5%) of either acetylsalicylic acid or acetic acid, or in saline solution (controls). Every five minutes, cell viability was tested (using the trypan blue test) and analyzed under light microscopy.
  
  
  RESULTS
  Tumor cell viability (in %) decreased progressively and, by 30 minutes, neoplastic cell death had occurred in all solutions.
  
  
  CONCLUSIONS
  Based on this experimental model and the methodology employed, we conclude that these solutions cause neoplastic cell death in vitro.
authors.affiliation: Department of Surgery and Orthopedics, Botucatu Medical School, Paulista State University, Brazil. saad@fmb.unesp.br
authors.forename: Rogério
authors.initials: R
authors.lastname: Saad-Hossne
authors.forename: René Gamberini
authors.initials: RG
authors.lastname: Prado
authors.forename: William Saad
authors.initials: WS
authors.lastname: Hossne
mesh_terms: D019342:Acetic Acid / Q000627:therapeutic use*; D000818:Animals; D001241:Aspirin / Q000627:therapeutic use*; D016923:Cell Death; D002470:Cell Survival / Q000187:drug effects; D004338:Drug Combinations; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D066298:In Vitro Techniques; D007202:Indicators and Reagents / Q000627:therapeutic use*; D008856:Microscopy, Fluorescence; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D011817:Rabbits
chemical_list: D004338:Drug Combinations; D007202:Indicators and Reagents; D019342:Acetic Acid; D001241:Aspirin