title: Effect of 2-deoxy-D-glucose on aminoacids metabolism in rats' cerebral cortex slices.
abstract: We studied the effect of different concentrations of 2-deoxy-D-glucose on the L-[U-14C]leucine, L-[1-14C]leucine and [1-14C]glycine metabolism in slices of cerebral cortex of 10-day-old rats. 2-deoxy-D-glucose since 0.5 mM concentration has inhibited significantly the protein synthesis from L-[U-14C]leucine and from [1-14C]glycine in relation to the medium containing only Krebs Ringer bicarbonate. Potassium 8.0 mM in incubation medium did not stimulate the protein synthesis compared to the medium containing 2.7 mM, and at 50 mM diminishes more than 2.5 times the protein synthesis compared to the other concentration. Only at the concentration of 5.0 mM, 2-deoxy-D-glucose inhibited the CO2 production and lipid synthesis from L-[U-14C] leucine. This compound did not inhibit either CO2 production, or lipid synthesis from [1-14C]glycine. Lactate at 10 mM and glucose 5.0 mM did not revert the inhibitory effect of 2-deoxy-D-glucose on the protein synthesis from L-[U-14C]leucine. 2-deoxy-D-glucose at 2.0 mM did not show any effect either on CO2 production, or on lipid synthesis from L-[U-14C]lactate 10 mM and glucose 5.0 mM.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Alexandre P
authors.initials: AP
authors.lastname: Muller
authors.forename: Liane N
authors.initials: LN
authors.lastname: Rotta
authors.forename: Cristina
authors.initials: C
authors.lastname: Kawano
authors.forename: Daniel N
authors.initials: DN
authors.lastname: Leszczinski
authors.forename: Ingrid D
authors.initials: ID
authors.lastname: Schweigert
authors.forename: Lisiane G
authors.initials: LG
authors.lastname: Londero
authors.forename: Fernanda S
authors.initials: FS
authors.lastname: Gravina
authors.forename: Clarice K B
authors.initials: CK
authors.lastname: da Silveira
authors.forename: Carolina G
authors.initials: CG
authors.lastname: de Souza
authors.forename: Cíntia E
authors.initials: CE
authors.lastname: Battu
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Gonçalves
authors.forename: Diogo O
authors.initials: DO
authors.lastname: de Souza
authors.forename: Marcos L S
authors.initials: ML
authors.lastname: Perry
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism*; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000378:metabolism; D007930:Leucine / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002250:Carbon Radioisotopes; D008055:Lipids; D002245:Carbon Dioxide; D019344:Lactic Acid; D003847:Deoxyglucose; D007930:Leucine; D005947:Glucose; D005998:Glycine