title: Coated wire potentiometric detection for capillary electrophoresis studied using organic amines, drugs, and biogenic amines.
abstract: Capillary electrophoresis was coupled successfully and reliably to potentiometric sensors, which are based on an ionically conductive rubber phase coating, applied on a 250 microm diameter metal substrate. The membrane components included potassium tetrakis(p-chlorophenyl)borate (TCPB), bis(2-ethylhexyl)sebacate (DOS), and high molecular mass poly(vinyl chloride) (PVC). Potentiometry reveals a very sensitive CE detection mode, with sub-micromolar detection limits for amines and the randomly chosen drugs quinine, clozapine, cocaine, heroine, noscapine, papaverine, and ritodrine. The lowest detection limit, 1 x 10(-8) M injected concentration, was obtained for the quaternary ammonium compound tetrahexylammonium chloride. The more polar lower aliphatic amines and the biogenic amines dopamine, adrenaline, and cadaverine have much higher detection limits. The detection limits are log P dependent. Addition of a commercially available calixarene molecule or a synthetic macrocyclic amphiphilic receptor molecule to the electrode coatings enhanced the sensitivity respectively for the lower aliphatic amines and for the biogenic amines. A transpose of the Nikolskii-Eisenman-type function was suggested and used to convert the signal of the detector to a concentration-dependent signal.
authors.affiliation: Chemistry Department, Antwerp University, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
authors.forename: Justyna
authors.initials: J
authors.lastname: Sekula
authors.forename: Joseph
authors.initials: J
authors.lastname: Everaert
authors.forename: Hugo
authors.initials: H
authors.lastname: Bohets
authors.forename: Bert
authors.initials: B
authors.lastname: Vissers
authors.forename: Marek
authors.initials: M
authors.lastname: Pietraszkiewicz
authors.forename: Oksana
authors.initials: O
authors.lastname: Pietraszkiewicz
authors.forename: Filip
authors.initials: F
authors.lastname: Du Prez
authors.forename: Koen
authors.initials: K
authors.lastname: Vanhoutte
authors.forename: Piotr
authors.initials: P
authors.lastname: Prus
authors.forename: Luc J
authors.initials: LJ
authors.lastname: Nagels
mesh_terms: D001679:Biogenic Amines / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D015394:Molecular Structure; D009930:Organic Chemicals / Q000737:chemistry*
chemical_list: D001679:Biogenic Amines; D009930:Organic Chemicals