title: RraA rescues Escherichia coli cells over-producing RNase E from growth arrest by modulating the ribonucleolytic activity.
abstract: RraA is an evolutionary conserved protein inhibitor of RNase E, which catalyzes the initial step in the decay and processing of numerous RNAs in Escherichia coli and forms the core component of the degradosome, a large protein complex involved in RNA metabolism. Here, we report that co-expression of RraA reduces the ribonucleolytic activity in cells over-producing RNase E and consequently rescues these cells from growth arrest. These findings suggest that inability of cells over-producing RNase E to normally grow results from increased cellular ribonucleolytic activity and RraA is able to effectively modulate the catalytic activity of RNase E in vivo.
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, Republic of Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Yeom
authors.forename: Kangseok
authors.initials: K
authors.lastname: Lee
mesh_terms: D002384:Catalysis; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D015151:Immunoblotting; D010957:Plasmids / Q000235:genetics; D012329:RNA, Bacterial / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014169:Transformation, Bacterial
chemical_list: D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; C477942:RraA protein, E coli; D004722:Endoribonucleases; C022537:ribonuclease E