Comparison of ELISA and capillary electrophoresis with laser-induced fluorescence detection in the analysis of Ochratoxin A in low volumes of human blood serum.
In this paper the determination of Ochratoxin A (OTA) in low volumes of human blood serum by enzyme-linked immunosorbent assay (ELISA) is compared with an appropriate capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method. In order to use ELISA for high-throughput analysis in epidemiological studies no sample cleanup was performed. Both methods showed a limit of detection (LOD) of 0.5 ng/mL. Comparing the precisions of both methods, the data show that the quantified concentrations in ELISA are higher than the corresponding concentrations in the CE-LIF method. Using a matrix calibration curve instead of a standard calibration curve the reproducibilities of both methods are comparable. No additional matrix effect could be observed by adding phenylalanine as probable matrix compound to the serum.
Köller
Gábor
G
Department of Human Exposure Research and Epidemiology, Centre for Environmental Research Leipzig-Halle, Permoserstr. 15, 04318 Leipzig, Germany. gabor.koeller@ufz.de
Wichmann
Gunnar
G
Rolle-Kampczyk
Ulrike
U
Popp
Peter
P
Herbarth
Olf
O
D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D009793:Ochratoxins / Q000097:blood* / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry; D015203:Reproducibility of Results; D044967:Serum; D013050:Spectrometry, Fluorescence / Q000379:methods*
D009793:Ochratoxins; C025589:ochratoxin A; D010649:Phenylalanine