title: Phosphoester-transfer mechanism of an RNA-cleaving acidic deoxyribozyme revealed by radioactivity tracking and enzymatic digestion.
abstract: A convenient method involving 32P-labeling of an RNA substrate at the cleavage site and subsequent enzymatic digestion of cleavage products via phosphatases reveals that pH4DZ1--an RNA-cleaving deoxyribozyme with optimal activity at pH 4--forms a 5'-cleavage fragment with 2',3'-cyclic phosphate group and a 3'-cleavage fragment with 5'-OH group.
authors.affiliation: Department of Biochemistry and Biomedical Sciences, Department of Chemistry, McMaster University, 1280 Main St. W. Hamilton, ON L8N 3Z5, Canada.
authors.forename: Srinivas A
authors.initials: SA
authors.lastname: Kandadai
authors.forename: William
authors.initials: W
authors.lastname: Chiuman
authors.forename: Yingfu
authors.initials: Y
authors.lastname: Li
mesh_terms: D021881:DNA, Catalytic / Q000737:chemistry*; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling; D010755:Organophosphates / Q000737:chemistry*; D010761:Phosphorus Radioisotopes / Q000737:chemistry; D012313:RNA / Q000737:chemistry*
chemical_list: D021881:DNA, Catalytic; D010755:Organophosphates; D010761:Phosphorus Radioisotopes; D012313:RNA