title: Milk fat progesterone concentrations in goats and early pregnancy diagnosis.
abstract: Blood and milk samples from foremilk during afternoon milking, were simultaneously collected from 285 dairy goats. In experiment 1, fiva cyclic goats were sampled daily for 21 days. In experiment 2, 280 females from 9 flocks were submitted to sampling 21 days after insemination. In addition, some milk samples were divided in two parts, after which one was frozen and the other kept at +4 degrees C until assay. Progesterone concentrations were measured in blood, whole milk and milk fat by radioimmunoassay. No difference in whole milk or fat progesterone levels was found between frozen and refrigerated milk samples. Milk butterfat progesterone concentrations paralleled those in plasma or whole milk throughout the estrous cycle and ranged from about 20 ng/ml at estrus to about 400 ng/ml in mid-luteal phase. The ratio of mid-luteal phase progesterone levels to those seen in the estrous period was over 20 in fat and in blood. This ratio was very much lower in whole milk. Consequently the determination of pregnant and non-pregnant goats from the samples collected 21 days after service was very much easier and accuracy was better when the progesterone content was assayed from milk fat than from whole milk. It was concluded that early pregnancy diagnosis in goats can be done routinely by determination of progesterone levels in milk fat.
authors.affiliation: Institut technique de l'Elevage Ovin et Caprin (ITOVIC) 149, rue de Bercy, 75595 Paris Cedex 12, France.
authors.forename: G
authors.initials: G
authors.lastname: de Montigny
authors.forename: P
authors.initials: P
authors.lastname: Millerioux
authors.forename: N
authors.initials: N
authors.lastname: Jeanguyot
authors.forename: P
authors.initials: P
authors.lastname: Humblot
authors.forename: M
authors.initials: M
authors.lastname: Thibier