Frequent rearrangements and amplification of the CDX2 homeobox gene in human sporadic colorectal cancers with chromosomal instability.
The expression of the CDX2 gene, a crucial regulator of gut homeostasis, is altered in human colorectal cancers in parallel with de-differentiation. Here, we have investigated the chromosomal status of CDX2 in human sporadic colorectal cancers with the phenotype of chromosomal instability. Allelic imbalance determination showed frequent rearrangements at the CDX2 locus. The rearrangements correlated with CDX2 gene amplification, as assessed by quantitative PCR analysis. However, they were not predictive of the Cdx2 protein pattern. These data suggest that mechanisms other than structural alterations at the CDX2 locus account for the change of expression in colorectal cancers.
Subtil
Clément
C
Inserm U682, University Louis Pasteur, 67200 Strasbourg, France.
Guérin
Eric
E
Schneider
Anne
A
Chenard
Marie-Pierre
MP
Martin
Elisabeth
E
Domon-Dell
Claire
C
Duluc
Isabelle
I
Brabletz
Thomas
T
Kedinger
Michèle
M
Duclos
Bernard
B
Gaub
Marie-Pierre
MP
Freund
Jean-Noël
JN
D001483:Base Sequence; D000071616:CDX2 Transcription Factor; D043171:Chromosomal Instability; D015179:Colorectal Neoplasms / Q000235:genetics*; D017931:DNA Primers; D005784:Gene Amplification; D015321:Gene Rearrangement; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans
D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D017931:DNA Primers; D018398:Homeodomain Proteins