Xenogenous fertilization of laboratory and domestic animals in the oviduct of the pseudopregnant rabbit.
Xenogenous fertilization was accomplished using bovine, porcine, and hamster follicular oocytes. The xenogenous fertilization rates for bovine and porcine follicular oocytes in the oviduct of the pseudopregnant rabbit were 13.4% and 2.0%, respectively. Temperatures of ovary, during transport to the laboratory, of 0 degrees or 37 degrees C had no effect on xenogenous fertilization rates of bovine oocytes. In vitro culture in 50 mug/ml FSH did not alter the xenogenous fertilization rates of bovine oocytes. Fertilization was observed with oocytes recovered 40 to 75 hr after insemination. Two cell embryos were recovered 70 to 75 hr after insemination. Ligation of the rabbit oviduct, number of ova deposited and sperm concentration did not affect the xenogenous fertilization rates of hamster ova. Cleavage of xenogenously fertilized hamster oocytes occurred between 28 and 29 hours after insemination.
Hirst
P J
PJ
Demayo
F J
FJ
Dukelow
W R
WR