Caldalkalibacillus thermarum gen. nov., sp. nov., a novel alkalithermophilic bacterium from a hot spring in China.
A thermophilic, alkaliphilic and catalase-positive bacterium, designated strain HA6(T), was isolated from a hot spring in China. The strain was aerobic and chemo-organotrophic and grew optimally at 60 degrees C, pH 8.5 and 1.5 % (w/v) NaCl. The cells were Gram-positive rods, forming single terminal endospores. The predominant cellular fatty acids were iso-C(15 : 0) and iso-C(17 : 0). The cell-wall peptidoglycan contained meso-diaminopimelic acid. The genomic DNA G+C content was 45.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain HA6(T) formed a distinct lineage within the family Bacillaceae and was most closely related to Bacillus horti K13(T) and Bacillus smithii DSM 4216(T), with sequence similarities of 91.8 and 93.1 %, respectively. On the basis of its physiological and molecular properties, strain HA6(T) should be placed in a novel genus and species, for which the name Caldalkalibacillus thermarum gen. nov., sp. nov. is proposed. The type strain of Caldalkalibacillus thermarum is strain HA6(T) (=CGMCC 1.4242(T)=JCM 13486(T)).
Xue
Yanfen
Y
State Key laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Zhang
Xinqi
X
State Key laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Zhou
Cheng
C
State Key laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Zhao
Yueju
Y
State Key laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Cowan
Don A
DA
Department of Biotechnology, University of the Western Cape, Bellville 7535, South Africa.
Heaphy
Shaun
S
Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, UK.
Grant
William D
WD
Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, UK.
Jones
Brian E
BE
Genencor International BV, Archimedesweg 30, 2333 CN Leiden, The Netherlands.
Ventosa
Antonio
A
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Ma
Yanhe
Y
State Key laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
D002681:China; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D016987:Gram-Positive Rods / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D045482:Hot Springs / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D013171:Spores, Bacterial; D014871:Water Microbiology
D005230:Fatty Acids, Nonesterified