title: Atypical Helicobacter canadensis strains associated with swine.
abstract: Forty-two Helicobacter isolates were isolated from swine feces in The Netherlands and Denmark. All 12 isolates sequenced (16S rRNA gene) formed a robust clade with Helicobacter canadensis ( approximately 99% similarity). Species-specific PCR indicated that all of the isolates were H. canadensis isolates. Although the appearance of the porcine isolates was similar to the appearance of H. canadensis, only one of these isolates was able to hydrolyze indoxyl acetate, a cardinal characteristic of this taxon. Examination of the 23S rRNA and hsp60 genes revealed high levels of similarity between the porcine isolates and H. canadensis. However, amplified fragment length polymorphism genomic typing showed that isolates recovered from swine feces were genetically distinct from H. canadensis strains obtained from humans and geese.
authors.affiliation: Food Safety and Quality National Program, Agriculture and Agri-Food Canada Research Centre, Alberta, T1J 4B1, Canada. inglisd@agr.gc.ca
authors.forename: G Douglas
authors.initials: GD
authors.lastname: Inglis
authors.forename: Malcolm
authors.initials: M
authors.lastname: McConville
authors.forename: Anno
authors.initials: A
authors.lastname: de Jong
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D016998:Helicobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013552:Swine / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S