A highly efficient method for liquid and solid cultivation of the anaerobic hyperthermophilic eubacterium Thermotoga maritima.
An efficient and economical medium--Thermotoga maritima basal medium (TMB)--was designed for the cultivation of T. maritima under either liquid or solid conditions. When the broth was flushed with N2 or CO2 throughout cell growth in a 10-L fermentor (pH controlled to 6.5), the maximum cell density (OD600) on TMB containing 1% glucose rose to 2.0 or higher (1.63 x 10(9) cells mL(-1)). Sheath-less cells observed by electron microscopy were captured during growth in the fermentor. Using a two-layer plating method, isolated single-well colonies were consistently obtained within 24 h on the TMB in modified tissue culture flasks. The minimal inhibitory chloramphenicol concentrations for T. maritima on TMB agar were 5 microg mL(-1) after 24 h and 48 h, and 25 microg mL(-1) at 72 h.
Jiang
Yu
Y
The Key Laboratory of Industrial Biotechnology Under the Ministry of Education, Southern Yangtze University, Wuxi, China.
Zhou
Qing
Q
Wu
Kang
K
Li
Xiang-Qian
XQ
Shao
Wei-Lan
WL
D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods*; D050260:Carbohydrate Metabolism; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006358:Hot Temperature; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D020124:Thermotoga maritima / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D003470:Culture Media