Phlorotannins in Ecklonia cava extract inhibit matrix metalloproteinase activity.
Matrix metalloproteinase (MMP) inhibitors have been identified as potential therapeutic candidates for metastasis, arthritis, chronic inflammation and wrinkle formation. For the first time here we report a detailed study on the inhibitory effects of phlorotannins in brown algae, Ecklonia cava (EC) on MMP activities in cultured human cell lines. A novel gelatin digestion assay could visualize complete inhibition of bacterial collagenase-1 activity at 20 microg/ml of EC extract during preliminary screening studies. Sensitive fluorometric assay revealed that EC extract can specifically inhibit both MMP-2 and MMP-9 activities significantly (P < 0.001) at 10 microg/ml. In addition, artificially induced activities of MMP-2 and MMP-9 in human dermal fibroblasts and HT1080 cells were inhibited by EC extract in a more or less similar manner to the positive control doxycycline. Even though the expression levels of MMPs differ from one cell type to the other, gelatin zymography clearly revealed that both MMP expression and activity in cells can be inhibited by EC extract. More interestingly, EC extract did not exert any cytotoxic effect even at 100 microg/ml anticipating its potential use as a safe MMP inhibitor.
Kim
Moon-Moo
MM
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
Ta
Quang Van
QV
Mendis
Eresha
E
Rajapakse
Niranjan
N
Jung
Won-Kyo
WK
Byun
Hee-Guk
HG
Jeon
You-Jin
YJ
Kim
Se-Kwon
SK
D001681:Biological Assay; D002478:Cells, Cultured; D017364:Collagenases; D004353:Drug Evaluation, Preclinical; D005347:Fibroblasts / Q000201:enzymology; D005470:Fluorometry; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D000459:Phaeophyceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D013634:Tannins / Q000032:analysis / Q000494:pharmacology*
D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D013634:Tannins; D017364:Collagenases; C417249:collagenase 1