Chromophores of allophycocyanin and R-phycocyanin.
The biliprotein allophycocyanin was purified from Phormidium luridum, Anabaena variabilis and Plectonema boryanum. R-phycocyanin was purified from Rhodymenia palmata. The chromophores were cleaved from the denatured protein by methanol hydrolysis. They were purified and crystallized as the dimethyl esters. Chromatographic and absorption-spectral (visible-ultraviolet and infrared) comparisons with reference material have established phycocyanobilin as the chromophore of allophycocyanin. Phycocyanobilin and phycoerythrobilin were shown to be the chromophores of R-phycocyanin.
Chapman
D J
DJ
Department of Biology, Brookhaven National Laboratory, Upton, N.Y. 11973, U.S.A.
Cole
W J
WJ
Siegelman
H W
HW