Effect of alloxan-diabetes on multiple forms of hexokinase in adipose tissue and lung.
Comparison has been made of the effect of alloxan-diabetes on the multiple forms of hexokinase (EC 2.7.1.1) in adipose tissue and lung. Types I and II hexokinase were distinguished in adipose tissue by their different stabilities to heat treatment, which made it possible to determine the activity of each form spectrophotometrically; additional confirmatory evidence was obtained from starch-gel electrophoresis. Type II hexokinase was markedly depressed in adipose tissue from alloxan-diabetic rats. Lung contained types I, II and III hexokinase, type I predominating. There was no significant change in the pattern of these multiple forms of hexokinase in lung from alloxan-diabetic rats. These results are discussed in relation to current ideas that the insulin-sensitivity of a tissue may be correlated with the content of type II hexokinase.
McLean
P
P
Courtauld Institute of Biochemistry, The Middlesex Hospital Medical School, London, W. 1, and Department of Medicine, University of California, Los Angeles, Calif. 90024, U.S.A.
Brown
J
J
Walters
E
E
Greenslade
K
K