title: Multiple copies of coding as well as pseudogene c-mos sequence exist in three lacertid species.
abstract: The analysis of a 581 bp section of the nuclear gene c-mos revealed multiple copies of putative functional sequences as well as pseudogenes in three closely related lacertid species Lacerta laevis, L. kulzeri and L. cyanisparsa. A phylogenetic analysis of c-mos in comparison with a molecular phylogeny based on the mitochondrial cytochrome b gene supports our findings. The study also provides new insights into the phylogenetic relationships of L. cyanisparsa and L. laevis. Pseudogenes of the three species share 11 single-nucleotide substitutions, a 1 bp deletion and a premature stop codon but differ by group-specific mutations. This result suggests that the c-mos gene has become duplicated and subsequently silenced already in the common ancestor of the three species. Sequence divergence suggests that the duplication and the loss of function occurred in the late Miocene/early Pliocene, i.e., about 5 million years ago. Indications of gene conversion are discussed. We suggest that future studies using c-mos for phylogenetic studies should provide evidence for the orthology of the sequences compared.
authors.affiliation: Laboratory of Molecular Systematics, Natural History Museum Vienna, Vienna, Austria. mihaela.pavlicev@nhm-wien.ac.at
authors.forename: Mihaela
authors.initials: M
authors.lastname: Pavlicev
authors.forename: Werner
authors.initials: W
authors.lastname: Mayer
mesh_terms: D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D017931:DNA Primers; D018628:Gene Dosage / Q000235:genetics*; D016258:Genes, mos / Q000235:genetics*; D014644:Genetic Variation; D008116:Lizards / Q000145:classification / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D011544:Pseudogenes / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers