title: Silver nanoparticle-based ultrasensitive chemiluminescent detection of DNA hybridization and single-nucleotide polymorphisms.
abstract: A new nanoparticle-based chemiluminescent (CL) method has been developed for the ultrasensitive detection of DNA hybridization. The assay relies on a sandwich-type DNA hybridization in which the DNA targets are first hybridized to the captured oligonucleotide probes immobilized on polystyrene microwells and then the silver nanoparticles modified with alkylthiol-capped oligonucleotides are used as probes to monitor the presence of the specific target DNA. After being anchored on the hybrids, silver nanoparticles are dissolved to Ag+ in HNO3 solution and sensitively determined by a coupling CL reaction system (Ag+-Mn2+-K2S2O8-H3PO4-luminol). The combination of the remarkable sensitivity of the CL method with the large number of Ag+ released from each hybrid allows the detection of specific sequence DNA targets at levels as low as 5 fM. The sensitivity increases 6 orders of magnitude greater than that of the gold nanoparticle-based colorimetric method and is comparable to that of surface-enhanced Raman spectroscopy, which is one of the most sensitive detection approaches available to the nanoparticle-based detection for DNA hybridization. Moreover, the perfectly complementary DNA targets and the single-base mismatched DNA strands can be evidently differentiated through controlling the temperature, which indicates that the proposed CL assay offers great promise for single-nucleotide polymorphism analysis.
authors.affiliation: College of Chemistry and Environment Science, Hebei University, Baoding 071002, China.
authors.forename: Cheng-Hui
authors.initials: CH
authors.lastname: Liu
authors.forename: Zheng-Ping
authors.initials: ZP
authors.lastname: Li
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Du
authors.forename: Xin-Rui
authors.initials: XR
authors.lastname: Duan
authors.forename: Yu-Cong
authors.initials: YC
authors.lastname: Wang
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013696:Temperature
chemical_list: D015342:DNA Probes; D012834:Silver; D004247:DNA