Decreased cADPR and increased NAD+ in the Cd38-/- mouse.
CD38 is a type II glycoprotein that catalyzes the formation of cyclic ADP-ribose (cADPR), an intracellular calcium signalling molecule, from nicotinamide adenine dinucleotide (NAD(+)). Using a modified version of the fluorimetric cycling assay for cADPR which reduces between-subject variability, we report significant decreases in brain and lung cADPR, which although similar to previously published values, showed much less individual variation. The reduced variation within each group suggests that the range of cADPR is narrower than previously thought, and that the regulatory mechanisms controlling these levels are more finely tuned. We also report significant increases in brain, lung, and kidney NAD(+) in the Cd38(-/-) mouse, and provide the first experimental demonstration of the proximate relationship between CD38 and NAD(+).
Young
Genevieve S
GS
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ont., Canada N1G 3W1. gyoung01@uoguelph.ca
Choleris
Elena
E
Lund
Frances E
FE
Kirkland
James B
JB
D051997:ADP-ribosyl Cyclase 1 / Q000172:deficiency*; D000818:Animals; D001921:Brain / Q000378:metabolism; D036563:Cyclic ADP-Ribose / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000172:deficiency*; D051379:Mice; D018345:Mice, Knockout; D009206:Myocardium / Q000378:metabolism; D009243:NAD / Q000378:metabolism*
D008562:Membrane Glycoproteins; D009243:NAD; D036563:Cyclic ADP-Ribose; C498155:Cd38 protein, mouse; D051997:ADP-ribosyl Cyclase 1