title: In vitro activity of an ear rinse containing tromethamine, EDTA, and benzyl alcohol on bacterial pathogens from dogs with otitis.
abstract: OBJECTIVE
  To evaluate the in vitro activity of an ear rinse (ER) containing tromethamine, EDTA, and benzyl alcohol on bacterial pathogens from dogs with otitis.
  
  
  METHODS
  Organisms were collected from ear swab specimens from the external and middle ear and included Staphylococcus spp (n = 11; Staphylococcus intermedius [7] and Staphylococcus spp [4]), Pseudomonas aeruginosa (5), Proteus spp (5), beta-hemolytic streptococcus (11), and 1 control strain of each organism.
  
  
  METHODS
  3 test solutions were evaluated including EDTA, tromethamine, and benzyl alcohol (ER); EDTA and tromethamine (ER without benzyl alcohol [ER - BA]); and purified water. Ten-milliliter aliquots of each test solution were transferred into 36 tubes and inoculated with one of the organisms. Samples were retrieved from each tube at 0, 15, 30, 45, and 60 minutes, transferred to Petri dishes, mixed with soybean-casein digest agar, and incubated. After incubation, plates were examined for growth, and the number of colonies was expressed as CFU per milliliter.
  
  
  RESULTS
  ER significantly decreased bacterial growth in vitro of P aeruginosa and beta-hemolytic streptococcal organisms within 15 minutes, Proteus spp within 30 minutes, and Staphylococcus spp within 60 minutes. Comparatively, the presence of benzyl alcohol in ER significantly decreased bacterial growth of beta-hemolytic streptococcus and Proteus spp.
  
  
  CONCLUSIONS
  On the basis of results of this study, future studies should be performed to evaluate the in vivo efficacy of ER alone as a treatment for otic infections caused by beta-hemolytic streptococcus, P aeruginosa, and Proteus spp and of ER combined with an antimicrobial agent for otic infections caused by Staphylococcus spp.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Lynette K
authors.initials: LK
authors.lastname: Cole
authors.forename: Dao H
authors.initials: DH
authors.lastname: Luu
authors.forename: Paivi J
authors.initials: PJ
authors.lastname: Rajala-Schultz
authors.forename: Cheyney
authors.initials: C
authors.lastname: Meadows
authors.forename: Audrey H
authors.initials: AH
authors.lastname: Torres
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects*; D019905:Benzyl Alcohol / Q000008:administration & dosage / Q000494:pharmacology*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D004304:Dosage Forms; D004492:Edetic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D010032:Otitis Externa / Q000382:microbiology / Q000662:veterinary*; D010033:Otitis Media / Q000382:microbiology / Q000662:veterinary*; D014325:Tromethamine / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D004304:Dosage Forms; D014325:Tromethamine; D004492:Edetic Acid; D019905:Benzyl Alcohol