In vitro fertilization of bovine follicular oocytes recovered by laparoscopy.
Thirty-three laparoscopies were performed on 16 follicle stimulating hormone (FSH)-superovulated cows (one to four laparoscopies per cow). Two hundred and four follicles were aspirated (6.18/cow) and 157 oocytes (77% recovery rate) were isolated. Sixty percent of the oocytes found were considered to be of good to excellent quality. In cows with ongoing ovulations at laparoscopy only 32% of the oocytes were suitable for fertilization. Twenty-four percent of the oocytes from cows with no ovulation and 16% from cows with ovulations were fertilized. Higher proportions of oocytes were fertilized in cows showing estrous behavior vs cows with no signs of estrus (25 vs 15%). Using lysolecithin-treated spermatozoa, 28% of the oocytes with good to excellent quality were fertilized, and 35% of the fertilized eggs cleaved in vitro to two- to four-cell stages.
Schellander
K
K
Department of Physiology and Pharmacology College of Veterinary Medicine The University of Georgia Athens, GA 30602 USA.
Fayrer-Hosken
R A
RA
Keefer
C L
CL
Brown
L M
LM
Malter
H
H
M C Bride
C E
CE
Brackett
B G
BG