Changes in biochemical parameters in rabbits blood after oral exposure to diphenyl diselenide for long periods.
The concept that selenium-containing molecules may be better antioxidants than classical antioxidants, has led to the design of synthetic organoselenium compounds. The present study was conducted to evaluate the potential toxicity of long time oral exposure to diphenyl diselenide (PhSe)2 in rabbits. Male adult New Zealand rabbits were divided into four groups, group I served as control; groups II, III and IV received 0.3, 3.0 and 30 ppm of (PhSe)2 pulverized in the chow for 8 months. A number of parameters were examined in blood as indicators of toxicity, including delta-aminolevulinate dehydratase (delta-ALA-D), catalase, glutathione peroxidase (GPx), alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, creatinine, TBARS, non-protein-SH, ascorbic acid and selenium. The results demonstrated that 6 and 8 months of 30 ppm (PhSe)2 intake caused a significant increase in blood delta-ALA-D activity. Erythrocyte non-protein thiol levels were significantly increased after 2 months of 30 ppm (PhSe)2 intake and then return to control levels after prolonged periods of intake. Ingestion of 3.0 ppm of (PhSe)2 for 8 months significantly increased catalase activity in erythrocytes. Conversely, no alterations in GPx, ALT, AST, TBARS and selenium levels were observed in rabbit serum, conversely, selenium levels in peri-renal adipose tissue were significantly increased after 8 months of 30 ppm (PhSe)2 intake, indicating its great lipophylicity. The present results suggest that diphenyl diselenide was not hepato- or renotoxic for rabbits, but caused some biochemical alterations that can be related to some pro-oxidant activity of the compound (particularly the reduction in Vitamin C).
de Bem
Andreza Fabro
AF
Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS 97105900, Brazil.
de Lima Portella
Rafael
R
Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS 97105900, Brazil.
Perottoni
Juliano
J
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS 97105900, Brazil.
Becker
Emilene
E
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS 97105900, Brazil.
Bohrer
Denise
D
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS 97105900, Brazil.
Paixão
Márcio Weber
MW
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS 97105900, Brazil.
Nogueira
Cristina Wayne
CW
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS 97105900, Brazil.
Zeni
Gilson
G
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS 97105900, Brazil.
Rocha
João Batista Teixeira
JBT
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS 97105900, Brazil. Electronic address: jbtrocha@yahoo.com.br.
D000284:Administration, Oral; D000821:Animal Feed; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood; D001555:Benzene Derivatives / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D001769:Blood / Q000187:drug effects* / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D003404:Creatinine / Q000652:urine; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D015394:Molecular Structure; D016566:Organoselenium Compounds / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D000623:Porphobilinogen Synthase / Q000378:metabolism; D011817:Rabbits; D012643:Selenium / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
D001555:Benzene Derivatives; D016566:Organoselenium Compounds; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; C061132:diphenyldiselenide; D003404:Creatinine; D002374:Catalase; D005979:Glutathione Peroxidase; D000623:Porphobilinogen Synthase; D012643:Selenium; D001205:Ascorbic Acid