title: Effect of gossypol and its metabolite on in vitro early development of mouse embryos.
abstract: The purpose of this study was to examine the effect of gossypol and its metabolite on early in vitro mouse embryo development. One hundred and thirty-eight excellent quality mouse blastocysts were randomly assigned to five different treatments. Culture media were supplemented with 10% (V/N) normal steer serum. The embryos were cultured at 37 degrees C with an atmosphere of 5% O(2), 5% CO(2) and 90% N(2), and embryo development was examined and recorded at 12-h intervals for 72 h. The percentage of embryos that developed to expanded blastocyst (92%), hatching blastocyst (84%), and hatched blastocyst (76%) stages in control Ham's F-10 media was not different from that of embryos cultured in media containing 0.1 and 5 mug of gossypol; however, none of the embryos treated with 265 ng of gossypol metabolite (GM) developed beyond the blastocyst stage. A substantial decrease in the percentage of embryos reaching hatching blastocyst (29%) and hatched blastocyst (29%) stages was observed in the embryos cultured with 5.3 ng of GM. At both light and electron microscopic levels, the embryos appeared to be affected even by a lower concentration of GM in vitro. Our results suggest that GM has a much greater potency than the parent gossypol in inhibiting the early development of mouse embryos in vitro.
authors.affiliation: Department of Veterinary Physiology and Pharmacology College of Veterinary Medicine The Ohio State University Columbus, OH 43210-1092 USA.
authors.forename: Y C
authors.initials: YC
authors.lastname: Lin
authors.forename: R
authors.initials: R
authors.lastname: Rajamahendran
authors.forename: Y
authors.initials: Y
authors.lastname: Rikihisa
authors.forename: F C
authors.initials: FC
authors.lastname: Gwazdauskas