Microwave assisted "click" chemistry for the synthesis of multiple labeled-carbohydrate oligonucleotides on solid support.
A versatile approach has been developed for the multiple labeling of oligonucleotides. First, three linkers as a H-phosphonate monoester derivative were condensed on a solid-supported T12 to introduce H-phosphonate diester linkages which were oxidized in the presence of propargylamine. Second, three galactosyl azide derivatives were conjugated to the solid-supported three-alkyne-modified T12 by a 1,3-cycloaddition so-called "click chemistry" in the presence of Cu(I) assisted by microwaves.
Bouillon
Camille
C
Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-Université Montpellier II, Place E. Bataillon, 34095 Montpellier Cedex 5, France.
Meyer
Albert
A
Vidal
Sébastien
S
Jochum
Anne
A
Chevolot
Yann
Y
Cloarec
Jean-Pierre
JP
Praly
Jean-Pierre
JP
Vasseur
Jean-Jacques
JJ
Morvan
François
F
D002241:Carbohydrates / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques; D003300:Copper; D008872:Microwaves; D015335:Molecular Probes / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis*
D002241:Carbohydrates; D015335:Molecular Probes; D009841:Oligonucleotides; D003300:Copper