Application of anodized aluminum in fluorescence detection of biological species.
Thin nanoporous alumina obtained by anodization of aluminum films offers promising advantages for application in fluorescence-based biological sensors including convenient preparation, increased density of binding sites, and improved collection efficiency of fluorescence. These advantages are illustrated in the detection of streptavidin using biotin covalently bound to the surface of alumina nanopores. Fluorescence intensity enhancement as high as 7 times is observed in nanopores in comparison to flat glass surface.
Takmakov
Pavel
P
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA.
Vlassiouk
Ivan
I
Smirnov
Sergei
S
D000535:Aluminum / Q000032:analysis* / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry; D007477:Ions / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D019809:Streptavidin / Q000737:chemistry
D007477:Ions; D012996:Solutions; D001710:Biotin; D019809:Streptavidin; D000535:Aluminum