Electric cell-substrate impedance sensing with screen printed electrode structures.
Impedance sensors in thick film technology have been tested as a tool for electric cell-substrate impedance sensing. The screen printed Pt electrodes have a width of 250-400 microm. Electrodes and the surrounding ceramic chip substrate could be homogeneously grown with L-929 and Hela cells. The performance of a screen printed interdigitated electrode structure (IDES) was compared with that of thin film structures with the same layout geometry. The thick film impedance sensors allowed to correctly record the morphological response of confluent Hela cell layers to stimulation with histamine. A thick film conductivity sensor also revealed impedance values which were dependent on cell growth on the electrode surface, even at a very low frequency range of approximately 1 Hz.
Brischwein
Martin
M
Heinz Nixdorf-Lehrstuhl für Medizinische Elektronik der Technischen Universität München, Theresienstrasse 90/N3, 80333 Munich, Germany. brischwein@tum.de
Herrmann
Sigrun
S
Vonau
Winfried
W
Berthold
Frank
F
Grothe
Helmut
H
Motrescu
Elena Roza
ER
Wolf
Bernhard
B
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D048708:Cell Growth Processes / Q000502:physiology; D002516:Ceramics / Q000737:chemistry; D017097:Electric Impedance; D004566:Electrodes; D005347:Fibroblasts / Q000166:cytology; D005898:Glass; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010984:Platinum / Q000737:chemistry
D010984:Platinum