title: Multiple actions of lysophosphatidylcholine in human Jurkat T cells.
abstract: OBJECTIVE
  To obtain pathophysiological meanings of lysophosphatidylcholine (LPC)through the investigation of the effects of LPC in Jurkat T cells .
  
  
  METHODS
  We measured ROS generation, [Ca(2+)](i), and mitochondrial membrane potential (MMP)by fluorescent spectrometry in Jurkat T cells.
  
  
  RESULTS
  We observed that LPC significantly increased the reactive oxygen species (ROS) level in human Jurkat T cells. Among structurally-related lysolipids and eleven synthetic LPCs with different acyl chain lengths, palmitoyl LPC increased ROS to the highest level. alpha-Tocopherol, an antioxidant, and rottlerin PKCdelta inhibitor were inhibitory effects on LPC-induced ROS generation. LPC rapidly depolarized MMP and markedly elevated [Ca(2+)](i) by Ca(2+) influx across the plasma membrane. However, LPC-induced ROS increase seemed to not be related with LPC-induced depolarization of MMP or [Ca(2+)](i) increase. G2A family G protein-coupled receptors (GPCR) for lysolipids were expressed in Jurkat T cells, however, evidence indicated that GPCR was not involved in LPC actions.
  
  
  CONCLUSIONS
  LPC induced several cellular changes in Jurkat T cells, including an increase of ROS generation in a PKCdelta-dependent and GPCR-independent manner, increase of [Ca(2+)](i) through Ca(2+) influx, and decrease of MMP. LPC-induced actions in Jurkat T cells represent novel action modes of LPC that do not involve GPCR and multiple independent changes of intracellular signaling molecules.
authors.affiliation: Laboratories of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 609-735, Korea.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Im
authors.forename: Yun-Kyung
authors.initials: YK
authors.lastname: Lee
authors.forename: Hae-Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Dong-Soon
authors.initials: DS
authors.lastname: Im
mesh_terms: D000098:Acetophenones / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001578:Benzopyrans / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D051745:Protein Kinase C-delta / Q000037:antagonists & inhibitors; D017382:Reactive Oxygen Species / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D024502:alpha-Tocopherol / Q000494:pharmacology
chemical_list: D000098:Acetophenones; D000975:Antioxidants; D001578:Benzopyrans; D008244:Lysophosphatidylcholines; D017382:Reactive Oxygen Species; D043562:Receptors, G-Protein-Coupled; C085746:rottlerin; D051745:Protein Kinase C-delta; D024502:alpha-Tocopherol; D002118:Calcium