title: Forward genetic screening of insertional mutants.
abstract: Insertional mutagenesis has contributed to the success of forward genetics in Arabidopsis thaliana. The availability of large collections of lines mutagenized by either transposon or T-DNA insertions, in combination with the systematic sequencing of insertion/genome junctions, enables the identification of mutations for any given gene in the nuclear genome of this plant species. Protocols for the identification and confirmation of mutations by forward genetics, the isolation of insertion/genome junctions, and the stabilization of transposon-induced mutations are provided, together with an overview of available insertional mutant collections and of their characteristics.
authors.affiliation: Botanisches Institut der Universität zu Köln, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Schneider
authors.forename: Dario
authors.initials: D
authors.lastname: Leister
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D004251:DNA Transposable Elements; D018744:DNA, Plant; D017343:Genes, Plant; D005821:Genetic Techniques; D008957:Models, Genetic; D016254:Mutagenesis, Insertional; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction
chemical_list: D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; D018744:DNA, Plant