Radical scavenging activity of ribonuclease inhibitor from cow placenta.
Cow placenta ribonuclease inhibitor (CPRI) has been purified 5062-fold by affinity chromatography, the product being homogeneous by sodium dodecyl sulfate-gel electrophoresis. The chemiluminescence technique was used to determine the radical scavenging activities of CPRI toward different reactive oxygen species (ROS) including superoxide anion (O2-*), hydroxyl radical (OH*), lipid-derived radicals (R*), and singlet oxygen (1O2). CPRI could effectively scavenge O2-*, OH*, R*, and 1O2 at EC50 of 0.12, 0.008, 0.009, and 0.006 mg/ml, respectively. In addition, the radical scavenging activities of CPRI were higher than those of tea polyphenols, indicating that CPRI is a powerful antioxidant.
Wang
Shuo
S
Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300222, P. R. China. s.wang@tust.edu.cn
Li
Haiping
H
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D002417:Cattle; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors / Q000378:metabolism; D008054:Lipid Peroxides / Q000037:antagonists & inhibitors / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology; D010920:Placenta / Q000737:chemistry*; D010926:Placental Hormones / Q000302:isolation & purification* / Q000494:pharmacology; D059808:Polyphenols; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D013481:Superoxides / Q000037:antagonists & inhibitors / Q000378:metabolism; D013662:Tea / Q000737:chemistry; D013997:Time Factors
D000975:Antioxidants; D004791:Enzyme Inhibitors; D005419:Flavonoids; D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D010636:Phenols; D010926:Placental Hormones; D059808:Polyphenols; D017382:Reactive Oxygen Species; D013662:Tea; D013481:Superoxides; C022052:placental ribonuclease inhibitor; D017665:Hydroxyl Radical