Deregulation of DNA damage signal transduction by herpesvirus latency-associated M2.
Infected cells recognize viral replication as a DNA damage stress and elicit a DNA damage response that ultimately induces apoptosis as part of host immune surveillance. Here, we demonstrate a novel mechanism where the murine gamma herpesvirus 68 (gammaHV68) latency-associated, anti-interferon M2 protein inhibits DNA damage-induced apoptosis by interacting with the DDB1/COP9/cullin repair complex and the ATM DNA damage signal transducer. M2 expression constitutively induced DDB1 nuclear localization and ATM kinase activation in the absence of DNA damage. Activated ATM subsequently induced Chk activation and p53 phosphorylation and stabilization without eliciting H2AX phosphorylation and MRN recruitment to foci upon DNA damage. Consequently, M2 expression inhibited DNA repair, rendered cells resistant to DNA damage-induced apoptosis, and induced a G(1) cell cycle arrest. Our results suggest that gammaHV68 M2 blocks apoptosis-mediated intracellular innate immunity, which might ultimately contribute to its role in latent infection.
Liang
Xiaozhen
X
Department of Microbiology and Molecular Genetics, New England Primate Research Center, Harvard Medical School, Southborough, MA 01772, USA.
Pickering
Mary T
MT
Cho
Nam-Hyuk
NH
Chang
Heesoon
H
Volkert
Michael R
MR
Kowalik
Timothy F
TF
Jung
Jae U
JU
D021581:Active Transport, Cell Nucleus; D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D002460:Cell Line; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism; D016193:G1 Phase; D006566:Herpesviridae Infections; D006801:Humans; D051379:Mice; D018144:Rhadinovirus / Q000502:physiology*; D015398:Signal Transduction; D014412:Tumor Virus Infections; D014763:Viral Matrix Proteins / Q000502:physiology*; D017735:Virus Latency
D004268:DNA-Binding Proteins; C490415:Ddb1 protein, mouse; D014763:Viral Matrix Proteins