title: Factors mediating lipofection potency of a series of cationic phosphonolipids in human cell lines.
abstract: A series of cationic liposomes known as cationic phosphonolipids (CPs) were evaluated as vehicles for in vitro gene transfer in K562 erythroleukemia cells and 5637 epithelial carcinoma cells. For each CP and target cell type examined, detailed analyses were performed to determine optimal transfection conditions (lipid/ DNA (+/-) charge ratio, amount of complexed episomal DNA, liposomal and lipoplex size, complexation medium and duration of complex-cell exposure time). Lipofection conditions were determined to be both cell- and lipid-type specific. Complexation medium critically affected transfection competence. The initial size of the liposome was not always predictive of lipofection potency. The lipid chemical composition had a strong impact upon lipofection efficiency; DOPE inclusion in the liposome formulations was found to affect the levels of transgene expression in a cell-dependent way. Notably, effective transgene expression was characterized by prominent plasmid nuclear incorporation. Human A gamma- and epsilon-globin transgene nuclear incorporation and expression in 5637 cells post GLB.391-mediated lipofection lends credence to its use as a vehicle of therapeutic transgene delivery.
authors.affiliation: First Department of Medicine, University of Athens, School of Medicine, Athens, Greece.
authors.forename: Daphne
authors.initials: D
authors.lastname: Koumbi
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Clement
authors.forename: Zili
authors.initials: Z
authors.lastname: Sideratou
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Yaouanc
authors.forename: Dimitris
authors.initials: D
authors.lastname: Loukopoulos
authors.forename: Panagoula
authors.initials: P
authors.lastname: Kollia
mesh_terms: D002412:Cations; D045744:Cell Line, Tumor; D006801:Humans; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry*; D010957:Plasmids; D014162:Transfection / Q000379:methods*
chemical_list: D002412:Cations; D010743:Phospholipids; C028299:phosphonolipids