Inactivation of bovine herpesvirus-1 (BHV-I) from in vitro infected bovine semen.
Frozen-thawed bovine semen, experimentally infected with bovine herpesvirus-1 (BHV-1) at levels of 10(3) TCID(50)/ml and 10(4) TCID(50)/ml, was treated with a 0.3% trypsin solution to determine the effect of trypsin on the virus and on fertilization using superovulated animals. Virus was not isolated from any trypsin-treated samples using a cell culture assay system. Nor did two calves develop antibodies to BHV-1 following inoculation with trypsin-treated semen pooled from six bulls. Nonsurgical flushing of eight heifers inseminated with trypsin-treated frozen-thawed semen yielded 28 transferable-quality embryos.
Bielanski
A
A
Agriculture Canada, Animal Diseases Research Institute, NEPEAN P.O. Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9.
Loewen
K G
KG
Hare
W C
WC