Inhibition of pig oocyte in vitro fertilization by the action of components of the zona pellucida.
The aim of the present study was to determine whether the previous addition of porcine zona pellucida (ZP) components to spermatozoa of the same species has an inhibitory effect on in vitro fertilization (IVF). Boar spermatozoa were exposed to whole porcine solubilized zona pellucida (SZP), ZP glycoproteins (55 kDa and 90 kDa) and peptides (37 kDa, 40 kDa and 68kDa). Doses tested were 40, 70 and 100 mug/ml. In vitro fertilization was clearly inhibited by each component when the oocytes were compared with those fertilized with untreated spermatozoa. All the components had an effect in a dose dependent manner.
Fierro
R
R
Departamento de Ciencias de la Salud Universidad Autónoma Metropolitana-Iztapalapa CP.09340 México, D.F., México.
Bonilla
E
E
Casas
E
E
Jiménez
I
I
Ducolomb
Y
Y
Betancourt
M
M