Effect of acidosis on expression of mesothelial cell plasminogen activator inhibitor type-1.
BACKGROUND
Abdominal mesothelial cells are exposed to carbon dioxide during laparoscopy. Previous data indicate that carbon dioxide increases release and expression of plasminogen activator inhibitor type-1 (PAI-1) and induces acidification.


METHODS
To assess the impact resulting from a range of pH, human mesothelial cells were exposed to culturing media balanced to pH levels of 6.0 to 8.0 for 90 min. Samples from cell media were withdrawn at several time points. Concentrations of PAI-1 and PAI-1 activity were measured using enzyme-linked immunoassay techniques. To focus on the effect of clinically relevant pH, cells were subjected to pH 6.4 and 7.4. Samples were withdrawn for PAI-1 assessments and for PAI-1 mRNA analyses.


RESULTS
During exposure to various levels of pH, PAI-1 secretion and activity were variable. However, 5 h after exposure, greater concentration and activity of PAI-1 were observed in acidified cultures. More PAI-1 mRNA was isolated after exposure of cells to a pH of 6.4, apparently indicating transcriptional regulation.


CONCLUSIONS
Mesothelial cells seem to respond to acidification by an increased release and production of PAI-1 in vitro.
Bergström
M
M
Department of Surgery, Sahlgrenska University Hospital, Göteborg University, S-41685, Göteborg, Sweden.
Falk
P
P
Holmdahl
L
L
D000138:Acidosis / Q000378:metabolism* / Q000473:pathology; D002245:Carbon Dioxide / Q000494:pharmacology; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010537:Peritoneum / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D002245:Carbon Dioxide