title: Detection of adeno-associated virus type 2 genome in cervical carcinoma.
abstract: Adeno-associated virus (AAV) can impair the replication of other viruses. Adeno-associated virus seroprevalences have been reported to be lower among women with cervical cancer. In-vitro, AAV can interfere with the production of human papillomavirus virions. Adeno-associated virus-2 DNA has also been detected in cervical cancer tissue, although not consistently. To evaluate the role of AAV infection in relation to invasive cervical cancer, we performed a nested case-control study within a retrospectively followed population-based cohort. A total of 104 women who developed invasive cervical cancer on average 5.6 years of follow-up (range: 0.5 months-26.2 years) and 104 matched control-women who did not develop cervical cancer during the same follow-up time were tested for AAV and human papillomavirus by polymerase chain reaction. At baseline, two (2%) case-women and three (3%) control-women were positive for AAV-2 DNA. At the time of cancer diagnosis, 12 (12%) case-women and 3 (3%) matched control-women were positive for AAV-2 DNA. Persisting AAV infection was not evident. In conclusion, AAV-2 DNA was present in a low proportion of cervical cancers and we found no evidence that the presence of AAV in cervical smears of healthy women would be associated with reduced risk of cervical cancer.
authors.affiliation: Department of Molecular Medicine and Surgery, Karolinska Institute, Center for Molecular Medicine, Karolinska University Hospital Solna, Stockholm, Sweden. biying.zheng@cmm.ki.se
authors.forename: B Y
authors.initials: BY
authors.lastname: Zheng
authors.forename: X D
authors.initials: XD
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Wiklund
authors.forename: S
authors.initials: S
authors.lastname: Chowdhry
authors.forename: T
authors.initials: T
authors.lastname: Angstrom
authors.forename: G
authors.initials: G
authors.lastname: Hallmans
authors.forename: J
authors.initials: J
authors.lastname: Dillner
authors.forename: K L
authors.initials: KL
authors.lastname: Wallin
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis*; D000229:Dependovirus / Q000235:genetics*; D005260:Female; D006801:Humans; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000453:epidemiology*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000821:virology*
chemical_list: D004279:DNA, Viral