Expression of genes coding for GerA and GerK spore germination receptors is dependent on the protein phosphatase PrpE.
The ability of Bacillus subtilis to form spores is a strategy for survival under unfavorable environmental conditions. It is equally crucial to break spore dormancy and return to vegetative growth at the appropriate time. Here we present data showing that the PrpE phosphatase is involved in the control of expression of genes coding for GerA receptors, which are necessary for L-alanine-induced spore germination. Moreover, PrpE is also involved in aspartic acid, glucose, fructose, and potassium (AGFK)-induced spore germination by controlling expression of genes coding for GerK receptors. In the absence of PrpE, the production of spores was essentially normal. However, L-alanine-induced spore germination and, to a lesser extent, the AGFK-induced pathway were abolished. In contrast, the germination pathway dependent on Ca2+-dipicolinate or dodecylamine remained intact. A protein phosphatase PrpE-green fluorescent protein fusion was localized to the prespore and to the dormant spore, consistent with a role in controlling expression of genes coding for GerA receptors. We propose that PrpE is an important element in a signal transduction pathway in Bacillus subtilis that controls the expression of genes coding for germination receptors.
Hinc
Krzysztof
K
Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-AMG, Medical University of Gdańsk, Debinki 1, 80-211 Gdańsk, Poland.
Nagórska
Krzysztofa
K
Iwanicki
Adam
A
Wegrzyn
Grzegorz
G
Séror
Simone J
SJ
Obuchowski
Michal
M
D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins / Q000235:genetics; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D013171:Spores, Bacterial / Q000235:genetics
D001426:Bacterial Proteins; C090771:GerAA protein, Bacillus subtilis; D008565:Membrane Proteins; D010749:Phosphoprotein Phosphatases