title: Fluorometric study on the interaction between lomefloxacin and bovine lactoferrin.
abstract: The binding of lomefloxacin to bovine lactoferrin (BLf) in a dilute aqueous solution was studied using fluorescence spectra. The binding constant (K) and the number of binding sites (n) were obtained by a fluorescence quenching method. The binding distance (r) and energy-transfer efficiency (E) between lomefloxacin and bovine lactoferrin were also obtained according to the mechanism of Foörster-type dipole-dipole nonradiative energy-transfer. The effect of lomefloxacin on the conformation of bovine lactoferrin was also analyzed by synchronous fluorescence spectroscopy. The interaction between lomefloxacin and bovine lactoferrin is strong. Lomefloxacin can affect the conformation of bovine lactoferrin to some degree.
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.forename: Ji-Cai
authors.initials: JC
authors.lastname: Fan
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Cheng-Ping
authors.initials: CP
authors.lastname: Fan
authors.forename: Zhi-Cai
authors.initials: ZC
authors.lastname: Shang
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004735:Energy Transfer; D024841:Fluoroquinolones / Q000737:chemistry* / Q000378:metabolism; D007781:Lactoferrin / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D024841:Fluoroquinolones; D007781:Lactoferrin; C053091:lomefloxacin