title: Endothelial response to stress from exogenous Zn2+ resembles that of NO-mediated nitrosative stress, and is protected by MT-1 overexpression.
abstract: While nitric oxide (NO)-mediated biological interactions have been intensively studied, the underlying mechanisms of nitrosative stress with resulting pathology remain unclear. Previous studies have demonstrated that NO exposure increases free zinc ions (Zn(2+)) within cells. However, the resulting effects on endothelial cell survival have not been adequately resolved. Thus the purpose of this study was to investigate the role of altered zinc homeostasis on endothelial cell survival. Initially, we confirmed the previously observed significant increase in free Zn(2+) with a subsequent induction of apoptosis in our pulmonary artery endothelial cells (PAECs) exposed to the NO donor N-[2-aminoethyl]-N-[2-hydroxy-2-nitrosohydrazino]-1,2-ethylenediamine. However, NO has many effects upon cell function and we wanted to specifically evaluate the effects mediated by zinc. To accomplish this we utilized the direct addition of zinc chloride (ZnCl(2)) to PAEC. We observed that Zn(2+)-exposed PAECs exhibited a dose-dependent increase in superoxide (O(2)(-).) generation that was localized to the mitochondria. Furthermore, we found Zn(2+)-exposed PAECs exhibited a significant reduction in mitochondrial membrane potential, loss of cardiolipin from the inner leaflet, caspase activation, and significant increases in TdT-mediated dUTP nick end labeling-positive cells. Furthermore, using an adenoviral construct for the overexpression of the Zn(2+)-binding protein, metallothionein-1 (MT-1), we found either MT-1 overexpression or coincubation with a Zn(2+)-selective chelator, N,N,N',N'-tetrakis(2-pyridylmethyl)ethylene-diamide, in PAECs significantly protected the mitochondria from both NO and Zn(2+)-mediated disruption and induction of apoptosis and cell death. In summary, our results indicate that a loss of Zn(2+) homeostasis produces mitochondrial dysfunction, increased oxidative stress, and apoptotic cell death. We propose that regulation of Zn(2+) levels may represent a potential therapeutic target for disease associated with both nitrosative and oxidative stress.
authors.affiliation: International Heart Institute, St. Patrick Hospital, 554 W. Broadway, Missoula, MT 59802, USA.
authors.forename: Dean A
authors.initials: DA
authors.lastname: Wiseman
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Wells
authors.forename: Jason
authors.initials: J
authors.lastname: Wilham
authors.forename: Maryann
authors.initials: M
authors.lastname: Hubbard
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Welker
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Black
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D002614:Chelating Agents; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005029:Ethylenediamines / Q000494:pharmacology; D005333:Fetus; D015870:Gene Expression; D006706:Homeostasis; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress; D011651:Pulmonary Artery / Q000166:cytology; D026361:Reactive Nitrogen Species / Q000378:metabolism; D012756:Sheep; D015032:Zinc / Q000633:toxicity*
chemical_list: D002614:Chelating Agents; D005029:Ethylenediamines; D026361:Reactive Nitrogen Species; D009569:Nitric Oxide; D008668:Metallothionein; D015032:Zinc; C044387:N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine