Genomic structure, promoter analysis, and expression of the porcine (Sus scrofa) Mx1 gene.
Allelic polymorphisms at the mouse Mx1 locus affect the probability of survival after experimental influenzal disease, raising the possibility that marker-assisted selection using the homologous locus could improve the innate resistance of pigs to natural influenza infections. Several issues need to be resolved before efficient large scale screening of the allelic polymorphism at the porcine (Sus scrofa) Mx1 locus can be implemented. First, the Mx1 genomic structure has to be established and sufficient flanking intronic sequences have to be gathered to enable simple PCR amplification of the coding portions of the gene. Then, a basic knowledge of the promoter region needs to be obtained as an allelic variation there can significantly alter absolute levels and/or tissue-specificity of MX protein expression. The results gathered here show that the porcine Mx1 gene and promoter share the major structural and functional characteristics displayed by their homologs described in cattle, mouse, chicken, and man. The crucial function of the proximal interferon-sensitive response elements motif for gene expression is also demonstrated. The sequence data compiled here will allow an extensive analysis of the polymorphisms present among the widest spectrum possible of porcine breeds with the aim to identify an Mx1 allele providing antiviral resistance.
Thomas
Anne V
AV
Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, Liège, 4000, Belgium.
Palm
Melanie
M
Broers
Aurore D
AD
Zezafoun
Hussein
H
Desmecht
Daniel J-M
DJ
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D019204:GTP-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D016678:Genome; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D064547:Myxovirus Resistance Proteins; D009976:Orthomyxoviridae Infections / Q000235:genetics* / Q000662:veterinary*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D034421:Sus scrofa / Q000235:genetics / Q000276:immunology*
C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins; D019204:GTP-Binding Proteins