Sequential uterine horn versus simultaneous total uterine flush to recover bovine embryos nonsurgically.
Beef cows were superovulated with follicle stimulating hormone (FSH) to compare two nonsurgical methods of embryo recovery from the uterus. In the first method each uterine horn was independently flushed with physiological saline solution (PSS) through a Foley catheter passed through the cervix and into the uterine horn. In the second method both uterine horns were simultaneously flushed with PSS by passing the catheter into the uterine body. In both methods, the numbers of ovulations were determined after embryo collection by counting the corpora lutea (CL) on both ovaries of each cow through a flank incision. Independent flushing (n = 19) averaged 6.4 embryos and 16.1 CL per cow for a recovery rate of 40%. Simultaneous flushings (n = 22) averaged 5.4 embryos and 17.7 CL per cow for a recovery rate of 31%. This difference between the recovery rates of the two flushing methods was not significant (P>0.05).
Hay
J H
JH
School of Veterinary Medicine University of Nevada, Reno, NV 89557 USA; Department of Animal Science University of Nevada, Reno, NV 89557 USA.
Phelps
D A
DA
Hanks
D R
DR
Foote
W D
WD