In vitro generation of tumor specific T cells that recognize a shared antigen of AML: molecular characterization of TCR genes.
The identification of immunologically relevant tumor antigens is hampered by the difficulty of generating tumor-specific cytotoxic T cells (CTL). We present data demonstrating in vitro induction of autologous acute myelogenous leukemia (AML)-specific CTL. The specific T cell receptor has been identified and cloned. The CTL demonstrated specific lysis to autologous tumor blasts, but not to autologous BLCL or the NK-sensitive target K562. The clone secreted GM-CSF, TNFa, and IFNg when stimulated with AML blasts from 3 of 11 patients or cell lines tested, but not with K562 or autologous B-LCL. These three AML samples share a single HLA Class I antigen, HLA-A24. The T cell receptor genes identified by molecular methods are Vbeta7.9-J2.3-Cbeta2 and Valpha17-J49-Calpha.
Coppage
Myra
M
University of Rochester Department of Pathology and Laboratory Medicine, 601 Elmwood Avenue, Rochester, NY 14642, United States. Sara.Rollinson@manchester.ac.uk
Belanger
Todd
T
Zauderer
Maurice
M
Sahasrabudhe
Deepak
D
D000208:Acute Disease; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D045744:Cell Line, Tumor; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000951:Antigens, Neoplasm; D006680:HLA Antigens; D011948:Receptors, Antigen, T-Cell