title: [Induction of hair follicle regeneration in mice ear by microencapsulated human hair dermal papilla cells].
abstract: OBJECTIVE
  To induce the hair follicle regeneration in mice ear by microencapsulated dermal papillae cells (DPs) and to investigate the permeability of fluorescein in APA microencapsulation to search the ideal diameter of microencapsulation.
  
  
  METHODS
  The DPs were encapsulated with alginate-polylysine-alginate by a high-voltage electric field droplet generator. The microencapsulated dermal papilla cells were xenotransplanted into the mice ears. After 6 week, the histological examination was made by microscopy. The diffusion way and speed of fluorescein into the microencapsulations were observed by confocal laser scanning microscopy. The comparison of fluorescein intensity was made in APA microencapsulations with different diameters.
  
  
  RESULTS
  Fully developed hair follicles could be easily identified in the skin of implanted site following xenotransplantation of microencapsulation DPs, which were different from the control groups in configuration, number, size and differentiation degree. The fluorescein was diffused gradually into the microencapsulations with a shape of concentric circularity. The fluorescein intensity inside three groups of APA microencapsulations was: small > middle > big.
  
  
  CONCLUSIONS
  The microencapsulated DPs retain the physiological function to induce the follicle regeneration. The APA microencapsulations with 400um diameter could ensure the nutrition and metabolite to pass in and out freely, and isolate the immunocompetent substance absolutely.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tissue Engineering laboraory, First Affiliated Hospital, Shantou University Medical College, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Chang-min
authors.initials: CM
authors.lastname: Lin
authors.forename: Xiang-na
authors.initials: XN
authors.lastname: Cai
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Li
authors.forename: Keng
authors.initials: K
authors.lastname: Huang
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D002454:Cell Differentiation; D017690:Cell Transplantation; D002478:Cells, Cultured; D004423:Ear; D005260:Female; D019793:Fluorescein / Q000737:chemistry; D018859:Hair Follicle / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011107:Polylysine / Q000031:analogs & derivatives / Q000737:chemistry; D012535:Scalp / Q000166:cytology* / Q000637:transplantation*
chemical_list: D000464:Alginates; C051546:alginate-polylysine-alginate; D011107:Polylysine; D019793:Fluorescein