A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis.
A simple and universally applicable protocol for extracting high-quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysis.
Wang
Wei
W
Dipartimento di Scienze Ambientali G. Sarfatti,Università di Siena, Italy. mails_wang@yahoo.com
Vignani
Rita
R
Scali
Monica
M
Cresti
Mauro
M
D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D010940:Plant Proteins / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry*; D040901:Proteomics; D016622:Silver Staining
D010940:Plant Proteins