title: Swelling-activated Cl- channels support Cl- secretion by bovine ciliary epithelium.
abstract: OBJECTIVE
  To determine whether swelling-activated Cl- currents (ICl,swell) observed in isolated nonpigmented ciliary epithelial (NPE) cells contribute to Cl- secretion across the ciliary epithelium.
  
  
  METHODS
  Ion transport across intact bovine ciliary epithelium was monitored electrically. Native isolated bovine NPE cells were harvested enzymatically. Cell volume changes were measured by calcein-fluorescence quenching.
  
  
  RESULTS
  Bilateral reduction in osmolality transiently increased short-circuit current (Isc), averaging 60% to 70%. Bilateral pretreatment with 5-nitro-2-(phenylpropylamino)-benzoate (NPPB), a Cl- channel blocker, reduced Isc stimulation by approximately 60%, suggesting that transcellular ICl,swell largely mediates the increased current. The hypotonically-triggered Isc stimulation was also inhibited by phloretin, a blocker of swelling-activated Cl- channels and by flufenamic acid, a blocker of Cl- and nonselective cation channels. Cyclamate substitution for bath Cl- reduced the baseline Isc and the increase in hypotonically-triggered Isc. In that case, addition of either NPPB or flufenamic acid did not produce further inhibition. The transepithelial responses were correlated with regulatory volume responses of freshly harvested NPE cells. Hypotonicity elicited a regulatory volume decrease (RVD) over a period comparable to that of the hypotonicity-triggered increase in Isc. The RVD was also inhibited by Cl--channel blockers and by Cl- substitution.
  
  
  CONCLUSIONS
  ICl,swell of NPE cells is functionally expressed in intact ciliary epithelium and is oriented to subserve aqueous humor formation. NPE cell volume can be measured with calcein-fluorescence quenching. ICl,swell may be stimulated by increased stromal fluid uptake and delivery to the NPE cells, facilitating Cl- secretion and increasing fluid release into the posterior chamber.
authors.affiliation: Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104-6085, USA.
authors.forename: Chi Wai
authors.initials: CW
authors.lastname: Do
authors.forename: Kim
authors.initials: K
authors.lastname: Peterson-Yantorno
authors.forename: Mortimer M
authors.initials: MM
authors.lastname: Civan
mesh_terms: D000818:Animals; D002417:Cattle; D048429:Cell Size / Q000187:drug effects; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism*; D002924:Ciliary Body / Q000166:cytology / Q000378:metabolism*; D004553:Electric Conductivity; D004847:Epithelial Cells / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005439:Flufenamic Acid / Q000494:pharmacology; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D015640:Ion Channel Gating; D017136:Ion Transport / Q000502:physiology; D009579:Nitrobenzoates / Q000494:pharmacology; D009994:Osmolar Concentration; D010693:Phloretin / Q000494:pharmacology
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009579:Nitrobenzoates; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D005439:Flufenamic Acid; D010693:Phloretin; C007740:fluorexon