Chromatin assembly of DNA templates microinjected into Xenopus oocytes.
The packaging of deoxyribonucleic acid (DNA) into chromatin within the eukaryotic nucleus can affect processes such as DNA replication, transcription, recombination, and repair. Therefore, studies aimed at understanding at the molecular level how these processes are operating have to take into account the chromatin context. We present a method to assemble DNA into chromatin by nuclear microinjection into Xenopus oocytes. This method allows in vivo chromatin formation in a nuclear environment. We provide the experimental procedures for oocyte preparation, DNA injection, and analysis of the assembled chromatin.
Roche
Danièle
D
Research Section, Institute Curie, UMR218 du Centre National de la Recherche Scientifique, Paris, France.
Almouzni
Geneviève
G
Quivy
Jean-Pierre
JP
D000818:Animals; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D004270:DNA, Circular / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D005260:Female; D008845:Microinjections / Q000379:methods*; D009865:Oocytes / Q000378:metabolism*; D013698:Templates, Genetic; D014981:Xenopus / Q000235:genetics
D004270:DNA, Circular; D004277:DNA, Single-Stranded