Fast 2D NMR ligand screening using Hadamard spectroscopy.
Fast 2D NMR-based screening can be achieved using Hadamard encoded spectroscopy to focus on the signals of interest (e.g., enzyme active or ligand recognition sites). By recording a set of Hadamard spectra (a "Hadamard constellation") with relative offsets comparable to the excitation bandwidth, quantitative ligand-induced shifts can be obtained from peak intensities.
Feliz
Miguel
M
Laboratory of Biomolecular NMR, Institute of Research in Biomedicine, Science Research Park, Josep Samitier 1-5, 08028 Barcelona, Spain.
García
Jesús
J
Aragón
Eric
E
Pons
Miquel
M
D002352:Carrier Proteins / Q000737:chemistry; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011485:Protein Binding
D002352:Carrier Proteins; D008024:Ligands