[Regulation of the expression of interleukin-4 and interleukin-5 by the signal pathway of PKC-NF-kappaB in T lymphocyte of allergic rhinitis].
OBJECTIVE
To explore the role of nuclear factor-kappaB (NF-kappaB) in the signal pathway of protein kinase C (PKC) regulating the expression of interleukin-4 (IL-4) and interleukin-5 (IL-5) by T lymphocyte in allergic rhinitis.


METHODS
Twenty-four allergic rhinitis patients and 24 normal control persons participated in the study. T lymphocytes were isolated and purified from blood of each person and divided into 3 groups: cells of 1st group were control group(Normal control and AR control),cells of 2nd group were stimulated with phorbol 12-myristate 13-acetate (Normal PMA and AR PMA),cells of 3rd group were stimulated with PMA and pyrrolidine dithiocarbamate (Normal PMA/PDTC and AR PMA/PDTC). The expression of NF-kappaB were observed by immunocytochemical staining, IL-4 and IL-5 protein in supernatants were measured by ELISA.


RESULTS
The percentage of cells of active NF-kappaB, IL-4 and IL-5 protein in supernatants of allergic rhinitis T lymphocyte stimulated with PMA were significantly higher than those of allergic rhinitis T lymphocyte stimulated without PMA ( P < 0.01) and with PMA and PDTC ( P < 0.01); than those of normal control T lymphocyte stimulated with PMA ( P < 0.01), with PMA and PDTC ( P < 0.01) and without PMA. The percentage of cells of active NF-kappaB, IL-4 and IL-5 protein in supernatants of allergic rhinitis T lymphocyte stimulated with PMA and PDTC were significantly lower than those of allergic rhinitis T lymphocyte stimulated with PMA (P < 0.01); but significantly higher than those of allergic rhinitis T lymphocyte stimulated without PMA and those of normal T lymphocyte stimulated. There were good positive correlation between the percentage of cells of active NF-kappaB and IL-4 and IL-5 protein in supernatants (r = 0.88,0.85, P < 0.01, respectively).


CONCLUSIONS
The active PKC of allergic rhinitis T lymphocyte increasing the expression of IL-4 and IL-5 may be mediated by activating NF-kappaB, the activation of PKC-NF-kappaB signal pathway of T lymphocyte NF-kappaB may play an important role in the pathogenesis of allergic rhinitis.
Liu
Jie
J
Department of Otorhinolaryngology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, China.
Liao
Hui
H
Zhang
Yamin
Y
Liu
Lisi
L
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism*; D015848:Interleukin-5 / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
C508598:IL4 protein, human; C508599:IL5 protein, human; D015848:Interleukin-5; D016328:NF-kappa B; D015847:Interleukin-4; D011493:Protein Kinase C