title: DNA analysis of digested tomato seeds in stomach contents.
abstract: Examination of stomach contents is one of the important steps in medical legal autopsy. Vegetative materials such as stems, roots, and seeds in stomach contents can be valuable evidence for providing investigative leads in death investigation. Currently, the identification of plant materials relies on microscopic and morphologic examination. We have found that many seeds are often protected from acid degradation during stomach digestion by their tough exterior seed coat. Tomato seeds were selected as a model system to assess DNA analysis and plant variety marker identification. The DNA-amplified fragment length polymorphism method was performed to determine if the DNA obtained from single seeds could be used for PCR analysis. From the amplified fragment length polymorphism results, some candidate markers for individualizing seeds from morphologically distinct tomatoes were identified. These data on DNA analysis of tomato seeds indicate amplified fragment length polymorphism is a viable procedure for the individualization of seeds from stomach contents in forensic investigations.
authors.affiliation: Nuclear Science Department, National Tsing Hua University, Hsinchu, Taiwan, ROC.
authors.forename: Cheng-Lung
authors.initials: CL
authors.lastname: Lee
authors.forename: Heather Miller
authors.initials: HM
authors.lastname: Coyle
authors.forename: Eric
authors.initials: E
authors.lastname: Carita
authors.forename: Carll
authors.initials: C
authors.lastname: Ladd
authors.forename: Nicholas C S
authors.initials: NC
authors.lastname: Yang
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Palmbach
authors.forename: Ian C
authors.initials: IC
authors.lastname: Hsu
authors.forename: Henry C
authors.initials: HC
authors.lastname: Lee
mesh_terms: D018744:DNA, Plant / Q000302:isolation & purification*; D005240:Feasibility Studies; D005554:Forensic Medicine / Q000379:methods*; D005766:Gastrointestinal Contents; D006801:Humans; D018551:Solanum lycopersicum / Q000235:genetics*; D008954:Models, Biological; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds / Q000235:genetics*
chemical_list: D018744:DNA, Plant