title: [Contribution of first polar body analysis to understanding of human aneuploidy mechanism].
abstract: In our species, reproduction failure rate is high. Clinical evidence is miscarriage where chromosomal origin was largely involved (66% of cases). The development of assisted reproduction techniques made possible to analyse unfertilized oocytes and preimplantation embryos. The results have shown a high rate of chromosome abnormalities before implantation (50%). Two mechanisms were identified which could generate aneuploidy, the meiosis non-disjunction and the premature separation of sister chromatids (PSSC). A FISH analysis of the first polar body, a cell complementary to the oocyte after meiosis, can be performed before intracytoplasmic sperm injection. We studied 2 distinct populations (patients over 38 years old and patients with recurrent implantation failure) and we confirmed that PSSC is the major mechanism linked to advanced maternal age in human.
authors.affiliation: Département de Biologie de la Reproduction, Cytogénétique, Génétique Médicale et Gynécologie Obstétrique, CHI Poissy Saint Germain, 10 rue du Champ Gaillard, 78303 Poissy Cedex.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Selva
authors.forename: Marianne
authors.initials: M
authors.lastname: Bergere
authors.forename: Denise
authors.initials: D
authors.lastname: Molina-Gomes
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Hammoud
authors.forename: Raoul
authors.initials: R
authors.lastname: Lombroso
authors.forename: François
authors.initials: F
authors.lastname: Vialard
mesh_terms: D000782:Aneuploidy; D002842:Chromatids / Q000235:genetics; D020090:Chromosome Segregation; D005260:Female; D006801:Humans; D008423:Maternal Age