Culture-independent detection of Eikenella corrodens and Veillonella parvula in primary endodontic infections.
Eikenella corrodens and Veillonella parvula are normal cultivable inhabitants of the oral cavity but their presence in endodontic infections has not been as common as it could be anticipated. This might have been because of shortcomings of culture techniques when it comes to bacterial isolation or identification. The present study intended to survey samples from primary endodontic infections for the presence of E. corrodens and V. parvula using a culture-independent 16S rRNA gene-based nested PCR protocol. Genomic DNA was isolated directly from samples taken from different forms of periradicular lesions, and the presence of E. corrodens and V. parvula was determined by nested PCR. Specificity for each primer pair was confirmed by sequence analysis of PCR products from positive clinical samples. V. parvula and E. corrodens were, respectively, detected in 33% and 14% of the root canals associated with chronic apical periodontitis. Both V. parvula and E. corrodens were found in 10% of the cases diagnosed as acute apical periodontitis. V. parvula and E. corrodens were detected in 21% and 26% of the samples from acute apical abscesses, respectively. In general, species-specific nPCR allowed the detection of V. parvula in 24% and E. corrodens in 18% of the samples taken from primary endodontic infections. Findings confirmed that V. parvula and E. corrodens can take part in the microbiota of primary endodontic infections, but in prevalence values somewhat higher when compared to most of the previous culture studies that had reported recovery of these species.
Rôças
I N
IN
Department of Endodontics, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
Siqueira
J F
JF
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016009:Chi-Square Distribution; D017931:DNA Primers / Q000235:genetics; D003786:Dental Pulp Cavity / Q000382:microbiology*; D016124:Eikenella corrodens / Q000235:genetics / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D014678:Veillonella / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers