Elimination and multiplication of bacteria during preparation and storage of buffy coat-derived platelet concentrates.
BACKGROUND
The prevalence of bacterial contamination of random-donor platelet concentrates (PCs) is considerably lower than that of blood donations. Which key steps of the preparation procedure contribute to the elimination of bacteria was investigated.


METHODS
Ten bacteria species were used. Blood donations were spiked with bacteria and stored at 22 degrees C for 8 hours. The buffy coats were kept for 6 hours. PCs were prepared from pools of 4 buffy coats. At each preparation step and during PC storage, bacteria contents were measured. In additional experiments, the titers of spiked blood and buffy coats were determined after storage at 20, 22, or 24 degrees C for 8 and up to 24 hours, respectively.


RESULTS
Enterobacter cloacae, Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, and Yersinia enterocolitica were completely inactivated during storage in blood or buffy coats. Titer reduction was between 3.32 and 4.62 log. Bacillus cereus, Propionibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis did not multiply. Compared with their values in spiked blood the titers in the PCs were reduced by 1.7 to 2.8 log. Klebsiella pneumoniae was the only species that grew in blood. With the exception of P. acnes, those species that were not removed by the preparation process multiplied in the PCs. Remarkable donor-to-donor variations of the bactericidal activities of buffy coats were detected when the storage time was prolonged to 24 hours.


CONCLUSIONS
Bacteria are significantly eliminated by the preparation procedure for random donor PCs. Also, blood and buffy coats are bactericidal for most species. When buffy-coat storage is prolonged, it cannot, however, be predicted whether specific strains vanish or multiply.
Mohr
Harald
H
Blood Center of the German Red Cross Chapters of NSTOB, Institutes Springe and Gera, Springe, Germany. hmohr@bsd-nstob.de
Bayer
Anette
A
Gravemann
Ute
U
Müller
Thomas H
TH
D001419:Bacteria / Q000254:growth & development*; D001792:Blood Platelets / Q000382:microbiology; D001793:Blood Preservation / Q000379:methods*; D006801:Humans; D050296:Microbial Viability; D010983:Plateletpheresis / Q000592:standards*; D013696:Temperature; D013997:Time Factors