Deep - freezing of rainbow trout Salmo gairdneri sperm at varying intervals after collection.
Rainbow trout semen cryopreserved 0.33, 5, 60 and 360 min after collection gave postthaw fertilization rates of 71.9, 68.4, 65.5 and 49.1% of eyed eggs, respectively. The corresponding result obtained with chilled semen stored for 2 wk under oxygen was 96.9%. This suggests that the freezing of rainbow trout semen should be done immediately after collection.
Schmidt-Baulain
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Institute of Animal Husbandry and Genetics University of Göttingen Albrecht-Thaer-Weg 1, D-3400 Göttingen Federal Republic of Germany.
Holtz
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