title: Low-potential nicotinamide adenine dinucleotide detection at a glassy carbon electrode modified with toluidine blue O functionalized multiwall carbon nanotubes.
abstract: The toluidine blue O (TBO) functionalized multiwall carbon nanotubes (MWNTs) nanomaterials (TBO-MWNTs) were prepared by assembling TBO onto the surface of a MWNTs modified glassy carbon (GC) electrode. Also TBO-MWNTs modified GC electrodes exhibiting a strong and stable electrocatalytic response toward beta-nicotinamide adenine dinucleotide (NADH) were described. Compared with a bare GC electrode, the TBO-MWNTs modified GC electrodes could decrease the oxidization overpotential of NADH by 730 mV, with a peak current at 0.0 V, since there was a positively synergistic electrocatalytic effect between the MWNTs and TBO toward NADH. Furthermore, the TBO-MWNTs modified GC electrodes had perfect performances, such as a low detection limit (down to 0.5 microM), being very stable (the current diminutions is lower than 6% in a period over 35 min), a fast response (within 3 s), and a wide linear range (from 2.0 microM to 3.5 mM). Such an ability of TBO-MWNTs to promote the NADH electron-transfer reaction suggests great promise for dehydrogenase-based amperometric biosensors.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University, Hunan, Changsha, P R China.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Zeng
authors.forename: Wanzhi
authors.initials: W
authors.lastname: Wei
authors.forename: Xiurong
authors.initials: X
authors.lastname: Zhai
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
mesh_terms: D002138:Calibration; D002244:Carbon / Q000737:chemistry*; D002384:Catalysis; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D008855:Microscopy, Electron, Scanning; D009243:NAD / Q000032:analysis* / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D013499:Surface Properties; D014048:Tolonium Chloride / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D009243:NAD; D014048:Tolonium Chloride; D002244:Carbon