Carbonic anhydrase activators: activation of isozyme XIII with amino acids and amines.
The first activation study of isoform XIII of carbonic anhydrase (CA, EC 4.2.1.1) is reported. A series of amino acids and amines incorporating protonatable moieties of the primary/heterocyclic amine type were included in the study. As for CA I and II, CA XIII activators enhance kcat and show no effect on KM, for the physiologic reaction catalyzed by this isoform. Excellent CA XIII activating properties were shown by D-amino acids (His, Phe, DOPA, and Trp), serotonin, and 4-(2-aminoethyl)-morpholine, whereas the corresponding L-amino acids, dopamine, histamine, and 1-(2-aminoethyl)-piperazine, were weaker activators.
Parkkila
Seppo
S
Institute of Medical Technology, University of Tampere, Tampere University Hospital, Biokatu 6, 33520 Tampere, Finland.
Vullo
Daniela
D
Puccetti
Luca
L
Parkkila
Anna-Kaisa
AK
Scozzafava
Andrea
A
Supuran
Claudiu T
CT
D000588:Amines / Q000494:pharmacology*; D000596:Amino Acids / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D020536:Enzyme Activators / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism*
D000588:Amines; D000596:Amino Acids; D020536:Enzyme Activators; D007527:Isoenzymes; D002256:Carbonic Anhydrases