Triterpenic acids and flavonoids from Satureja parvifolia. Evaluation of their antiprotozoal activity.
Bioassay-guided fractionation of a Satureja parvifolia MeOH extract led to the isolation of eriodictyol, luteolin and ursolic and oleanolic acids as its active components against Plasmodium falciparum K1. This is the first time these compounds are reported as constituents of S. parvifolia. Ursolic acid showed an IC50 of 4.9 microg/ml, luteolin 6.4 microg/ml, oleanolic acid 9.3 microg/ml and eriodictyol 17.2 microg/ml. Antiplasmodial activity of eriodictyol and luteolin is reported here for the first time. Besides, the four compounds showed activity against P. falciparum 3D7 strain and Trypanosoma brucei rhodesiense. Eriodictyol showed moderate activity on all the parasites but was the most selective compound as a result of its rather low cytotoxicity (IC50 174.2 microg/ml) on the mammalian KB cell line.
van Baren
Catalina
C
Cátedra de Farmacognosia, Instituto de Química y Metabolismo del Fármaco IQUIMEFA (UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, República Argentina.
Anao
Ivie
I
Leo Di Lira
Paola
P
Debenedetti
Silvia
S
Houghton
Peter
P
Croft
Simon
S
Martino
Virginia
V
D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects*; D002738:Chloroquine / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D044950:Flavanones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D000432:Methanol; D009828:Oleanolic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008517:Phytotherapy; D035261:Plant Components, Aerial / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects*; D031362:Satureja / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000097245:Ursolic Acid
D000981:Antiprotozoal Agents; D002738:Chloroquine; D044950:Flavanones; D005419:Flavonoids; D000432:Methanol; D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid; C007619:eriodictyol