title: Antigen selection in the IgE response of allergic and nonallergic individuals.
abstract: BACKGROUND
  Affinity maturation within germinal centers should usually lead to an accumulation of replacement mutations in complementarity-determining regions (CDRs) of Ig genes as a result of antigen selection. A number of studies have suggested, but not statistically demonstrated, that antigen selection might not guide such an accumulation of replacement mutations in allergic IgE sequences. This has been suggested to reflect the nature of allergens themselves or of the allergic response.
  
  
  OBJECTIVE
  We sought to investigate the role of antigen selection in the evolution of the IgE response by mean of analysis of Ig sequences derived from both allergic and nonallergic individuals.
  
  
  METHODS
  IgE sequences were amplified from peripheral blood of allergic and nonallergic individuals by using seminested RT-PCR. Additional IgE and IgG sequences were obtained from public databases. Analysis considered replacement mutations in the CDRs as a proportion of total mutations and compared IgE sequences with IgG sequences.
  
  
  RESULTS
  The nonallergic IgE sequences were significantly less mutated than both the allergic IgE (P < .001) and IgG (P < .01) sequences. There was a low proportion of replacement mutations in the CDRs of both nonallergic and allergic IgE sequences and a significantly increased proportion of such mutations in IgG sequences (P < .001).
  
  
  CONCLUSIONS
  IgE antibodies in both nonallergic and allergic individuals appear to accumulate few somatic point mutations as a consequence of antigen selection.
  
  
  CONCLUSIONS
  Allergic and nonallergic IgE responses might often develop along a common pathway that is distinct from the conventional germinal center reaction through which the IgG response develops.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydeny, NSW, Australia.
authors.forename: Isabell
authors.initials: I
authors.lastname: Dahlke
authors.forename: David J
authors.initials: DJ
authors.lastname: Nott
authors.forename: John
authors.initials: J
authors.lastname: Ruhno
authors.forename: William A
authors.initials: WA
authors.lastname: Sewell
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Collins
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology*; D000941:Antigens / Q000235:genetics / Q000276:immunology*; D004252:DNA Mutational Analysis; D015723:Gene Library; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000235:genetics*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000485:Allergens; D000941:Antigens; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007073:Immunoglobulin E