title: Effects of LH and FSH on the maturation of pig oocytes in vitro.
abstract: This research was designed to investigate the effects of LH and FSH (50 ng/ml) on pig oocyte maturation in vitro. The following parameters were studied: a) the degree of heterologous coupling between cumulus cells and oocytes, evaluated by measuring the 3H-uridine and 3H-choline uptake in cumulus enclosed oocytes; b) meiotic maturation; c) cytoplasmatic maturation, evaluated by analyzing the ability of the oocytes to promote male pronucleus formation after in vitro fertilization. Despite the marked cumuli expansion induced by gonadotropins, uridine uptake was not influenced by LH or FSH. By contrast, choline uptake in LH-treated oocytes was significantly higher than in FSH-treated or control oocytes (3199 cpm+/-251 vs 1686 cpm+/-142, P<0.01). Gonadotropins accelerated meiotic progression, and after 30 hours of culture the percentage of oocytes at the germinal vesicle stage was significantly lower (P<0.01) in LH-(24%, 24/102) and FSH-(20%, 18/90) treated oocytes than in control oocytes (76%, 64/84). After 44 hours of culture, the percentage of oocytes reaching the MII stage was significantly higher (P<0.01) in the presence of LH (76%, 92/120) and FSH (86%, 92/108) than in the controls (35%, 40/116). The percentage of oocytes capable of sustaining male pronucleus formation was similar in the control (48.4%, 63/132) and FSH-treated oocytes (44.3%, 51/116), while it was markedly increased (P<0.01) by the addition of LH (72.7%, 143/197). The data reported indicate that in vitro pig oocytes tend to undergo meiotic maturation even in the absence of hormones. However, in our in vitro system, LH and FSH accelerated and facilitated meiotic progression, and LH selectively improved cytoplasmic maturation which is required to promote the formation of a male pronucleus.
authors.affiliation: Istituto di Fisiologia Veterinaria, Via Belmeloro 8/2, 40126, Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mattioli
authors.forename: M L
authors.initials: ML
authors.lastname: Bacci
authors.forename: G
authors.initials: G
authors.lastname: Galeati
authors.forename: E
authors.initials: E
authors.lastname: Seren