title: Reduction of acid tolerance by tetracycline in Escherichia coli expressing tetA(C) is reversed by cations.
abstract: When tetracycline was present, tetA(C) reduced acid tolerance, suppressed rpoS expression, and increased the concentration of total soluble proteins in stationary-phase Escherichia coli. The suppression of acid tolerance was reversed by 85 mM sodium, potassium, magnesium, and calcium ions but not by 85 mM sucrose. Implications for using TetA(C) are discussed.
authors.affiliation: Department of Food Microbiology and Toxicology, Food Research Institute, University of Wisconsin, 1925 Willow Dr., Madison, WI 53706-1187, USA.
authors.forename: Kai F
authors.initials: KF
authors.lastname: Hung
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Byrd
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Bose
authors.forename: Charles W
authors.initials: CW
authors.lastname: Kaspar
mesh_terms: D017920:Antiporters / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002412:Cations / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D013395:Sucrose / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology*
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D002412:Cations; D029968:Escherichia coli Proteins; C079051:tetA protein, Bacteria; D013395:Sucrose; D013752:Tetracycline