A combinatorial approach to minimal peptide models of a metalloprotein active site.
Screening of a "one-bead-one-compound" peptide library containing biomimetic His/Cys ligands has led to the discovery of sequences that hydrolyze ester substrates in combination with Zn2+.
Namuswe
Frances
F
Department of Chemistry, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA.
Goldberg
David P
DP
D000595:Amino Acid Sequence; D001665:Binding Sites; D020650:Combinatorial Chemistry Techniques; D003545:Cysteine / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D006868:Hydrolysis; D008024:Ligands; D008667:Metalloproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009596:Nitrophenols / Q000737:chemistry; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D020539:Sequence Analysis, Protein; D013997:Time Factors; D015032:Zinc / Q000737:chemistry*
D008024:Ligands; D008667:Metalloproteins; D009596:Nitrophenols; D019151:Peptide Library; D010455:Peptides; D006639:Histidine; C008642:4-nitrophenyl acetate; D015032:Zinc; D003545:Cysteine