Restraint stress modulates glucocorticoid receptors and nuclear factor kappa B in the cochlea.
The regulation of glucocorticoid receptors and nuclear factor kappaB was evaluated in the spiral ganglion neurons after 4 h of restraint stress in the mouse cochlea. Immediately after restraint stress, glucocorticoid receptor protein expression was not altered in spiral ganglion neurons even though both the plasma corticosterone levels and glucocorticoid receptor nuclear translocation increased. By 24 h after restraint stress, the protein expression of glucocorticoid receptors was decreased in spiral ganglion neurons. Pre-treatment with RU486 and metyrapone prevented nuclear translocation of glucocorticoid receptors and nuclear factor kappaB. Moreover, the synthesis of nuclear factor kappaB protein (p65) and inhibitory factor kappaBalpha decreased when RU486 and metyrapone treatment was given before restraint stress. These findings suggest that restraint stress modulates glucocorticoid receptor and nuclear factor kappaB activity in the spiral ganglion neurons, resulting in an altered response to stress.
Tahera
Yeasmin
Y
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Meltser
Inna
I
Johansson
Peter
P
Canlon
Barbara
B
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D003051:Cochlea / Q000378:metabolism*; D003345:Corticosterone / Q000097:blood; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008797:Metyrapone / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D015735:Mifepristone / Q000494:pharmacology; D016328:NF-kappa B / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D012149:Restraint, Physical / Q000379:methods; D013136:Spiral Ganglion / Q000473:pathology; D013315:Stress, Psychological / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors
D004791:Enzyme Inhibitors; D016328:NF-kappa B; D011965:Receptors, Glucocorticoid; D015735:Mifepristone; D003345:Corticosterone; D008797:Metyrapone