Expression of type XXIII collagen mRNA and protein.
Collagen XXIII is a member of the transmembranous subfamily of collagens containing a cytoplasmic domain, a membrane-spanning hydrophobic domain, and three extracellular triple helical collagenous domains interspersed with non-collagenous domains. We cloned mouse, chicken, and humanalpha1(XXIII) collagen cDNAs and showed that this non-abundant collagen has a limited tissue distribution in non-tumor tissues. Lung, cornea, brain, skin, tendon, and kidney are the major sites of expression. In contrast, five transformed cell lines were tested for collagen XXIII expression, and all expressed the mRNA. In vivo the alpha1(XXIII) mRNA is found in mature and developing organs, the latter demonstrated using stages of embryonic chick cornea and mouse embryos. Polyclonal antibodies were generated in guinea pig and rabbit and showed that collagen XXIII has a transmembranous form and a shed form. Comparison of collagen XXIII with its closest relatives in the transmembranous subfamily of collagens, types XIII and XXV, which have the same number of triple helical and non-collagenous regions, showed that there is a discontinuity in the alignment of domains but that striking similarities remain despite this.
Koch
Manuel
M
Center for Biochemistry, University of Cologne, Joseph-Stelzmann Strasse 52, 50931 Cologne, Germany; Department of Dermatology, University of Cologne, Joseph-Stelzmann Strasse 52, 50931 Cologne, Germany; Center for Molecular Medicine Cologne, University of Cologne, Joseph-Stelzmann Strasse 52, 50931 Cologne, Germany.
Veit
Guido
G
Center for Biochemistry, University of Cologne, Joseph-Stelzmann Strasse 52, 50931 Cologne, Germany.
Stricker
Sigmar
S
Development and Disease Group, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.
Bhatt
Pinaki
P
Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy and the Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, New Jersey 08854.
Kutsch
Stefanie
S
Center for Biochemistry, University of Cologne, Joseph-Stelzmann Strasse 52, 50931 Cologne, Germany.
Zhou
Peihong
P
Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy and the Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, New Jersey 08854.
Reinders
Elina
E
Center for Biochemistry, University of Cologne, Joseph-Stelzmann Strasse 52, 50931 Cologne, Germany.
Hahn
Rita A
RA
Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy and the Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, New Jersey 08854.
Song
Rich
R
Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy and the Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, New Jersey 08854.
Burgeson
Robert E
RE
Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy and the Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, New Jersey 08854.
Gerecke
Donald R
DR
Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy and the Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, New Jersey 08854.
Mundlos
Stefan
S
Development and Disease Group, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany; Institute for Medical Genetics, University Medicine Charité, 13353 Berlin, Germany.
Gordon
Marion K
MK
Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy and the Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, New Jersey 08854. Electronic address: magordon@eohsi.rutgers.edu.
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D003094:Collagen / Q000096:biosynthesis* / Q000737:chemistry*; D005786:Gene Expression Regulation; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
C499252:COL23A1 protein, human; D011994:Recombinant Proteins; D003094:Collagen