Global analysis of gene function in yeast by quantitative phenotypic profiling.
We present a method for the global analysis of the function of genes in budding yeast based on hierarchical clustering of the quantitative sensitivity profiles of the 4756 strains with individual homozygous deletion of nonessential genes to a broad range of cytotoxic or cytostatic agents. This method is superior to other global methods of identifying the function of genes involved in the various DNA repair and damage checkpoint pathways as well as other interrogated functions. Analysis of the phenotypic profiles of the 51 diverse treatments places a total of 860 genes of unknown function in clusters with genes of known function. We demonstrate that this can not only identify the function of unknown genes but can also suggest the mechanism of action of the agents used. This method will be useful when used alone and in conjunction with other global approaches to identify gene function in yeast.
Brown
James A
JA
Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305-5152, USA. mbrown@stanford.edu
Sherlock
Gavin
G
Myers
Chad L
CL
Burrows
Nicola M
NM
Deng
Changchun
C
Wu
H Irene
HI
McCann
Kelly E
KE
Troyanskaya
Olga G
OG
Brown
J Martin
JM
D016000:Cluster Analysis; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D017353:Gene Deletion; D005800:Genes, Fungal / Q000502:physiology*; D008722:Methods; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*