title: Germ cell development in equine testis tissue xenografted into mice.
abstract: Grafting of testis tissue from immature animals to immunodeficient mice results in complete spermatogenesis, albeit with varying efficiency in different species. The objectives of this study were to investigate if grafting of horse testis tissue would result in spermatogenesis, and to assess the effect of exogenous gonadotropins on xenograft development. Small fragments of testis tissue from 7 colts (2 week to 4 years of age) were grafted under the back skin of castrated male immunodeficient mice. For 2 donor animals, half of the mice were treated with gonadotropins. Xenografts were analyzed at 4 and 8 months post-transplantation. Spermatogenic differentiation following grafting ranged from no differentiation to progression through meiosis with appearance of haploid cells. Administration of exogenous gonadotropins appeared to support post-meiotic differentiation. For more mature donor testis samples where spermatogenesis had progressed into or through meiosis, after grafting an initial loss of differentiated germ cells was observed followed by a resurgence of spermatogenesis. However, if haploid cells had been present prior to grafting, spermatogenesis did not progress beyond meiotic division. In all host mice with spermatogenic differentiation in grafts, increased weight of the seminal vesicles compared to castrated mice showed that xenografts were releasing testosterone. These results indicate that horse spermatogenesis occurs in a mouse host albeit with low efficiency. In most cases, spermatogenesis arrested at meiosis. The underlying mechanisms of this spermatogenic arrest require further investigation.
authors.affiliation: Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, 19348, USA.
authors.forename: R
authors.initials: R
authors.lastname: Rathi
authors.forename: A
authors.initials: A
authors.lastname: Honaramooz
authors.forename: W
authors.initials: W
authors.lastname: Zeng
authors.forename: R
authors.initials: R
authors.lastname: Turner
authors.forename: I
authors.initials: I
authors.lastname: Dobrinski
mesh_terms: D000818:Animals; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006085:Graft Survival; D008297:Male; D008540:Meiosis; D051379:Mice; D016513:Mice, SCID; D009919:Orchiectomy; D012671:Seminiferous Tubules / Q000254:growth & development; D013091:Spermatogenesis; D013094:Spermatozoa / Q000166:cytology*; D013737:Testis / Q000378:metabolism / Q000637:transplantation*; D013739:Testosterone / Q000096:biosynthesis; D014183:Transplantation, Heterologous
chemical_list: D006064:Gonadotropins, Equine; D013739:Testosterone