Imaging cerebral blood flow through the intact rat skull with temporal laser speckle imaging.
We discovered that laser speckle temporal contrast analysis (LSTCA) is able to access the two-dimensional (2D) cerebral blood flow velocity and vessel structure through the intact rat skull. It is demonstrated that LSTCA can significantly suppress the influence of the laser speckle from the stationary structure, such as the skull, and thus reveal the blood flow and morphology of blood vessels through the laser speckle images recorded from the intact rat skull.
Li
Pengcheng
P
Key Laboratory of Biomedical Photonics of Ministry of Education--Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China.
Ni
Songlin
S
Zhang
Li
L
Zeng
Shaoqun
S
Luo
Qingming
Q
D000465:Algorithms; D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000166:cytology / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D007834:Lasers; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted; D012886:Skull / Q000098:blood supply / Q000166:cytology / Q000502:physiology