Effect of salt nutrients on mannitol production by Lactobacillus intermedius NRRL B-3693.
The effects of four salt nutrients (ammonium citrate, sodium phosphate, magnesium sulfate, and manganese sulfate) on the production of mannitol by Lactobacillus intermedius NRRL B-3693 in a simplified medium containing 300 g fructose, 5 g soy peptone, and 50 g corn steep liquor per liter in pH-controlled fermentation at 5.0 at 37 degrees C were evaluated using a fractional factorial design. Only manganese sulfate was found to be essential for mannitol production. Added manganese sulfate concentration of 0.033 g/l was found to support maximum production. The bacterium produced 200.6 +/- 0.2 g mannitol, 61.9 +/- 0.1 g lactic acid, and 40.4 +/- 0.3 g acetic acid from 300 g fructose per liter in 67 h.
Saha
Badal C
BC
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA. sahabc@ncaur.usda.gov
D001709:Biotechnology / Q000379:methods*; D019343:Citric Acid / Q000032:analysis / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D007218:Industrial Microbiology / Q000379:methods*; D007778:Lactobacillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008278:Magnesium Sulfate / Q000032:analysis / Q000494:pharmacology; D017895:Manganese Compounds / Q000032:analysis / Q000494:pharmacology; D008353:Mannitol / Q000378:metabolism*; D010710:Phosphates / Q000032:analysis / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000032:analysis / Q000494:pharmacology; D012492:Salts / Q000032:analysis / Q000494:pharmacology*; D013431:Sulfates / Q000032:analysis / Q000494:pharmacology
D003470:Culture Media; D017895:Manganese Compounds; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D012492:Salts; D013431:Sulfates; D019343:Citric Acid; D008353:Mannitol; D008278:Magnesium Sulfate; C481046:ammonium citrate; C018279:sodium phosphate; C039798:manganese sulfate