title: Activation of pyruvate dehydrogenase in perfused rat heart by dichloroacetate (Short Communication).
abstract: The activity of pyruvate dehydrogenase was assayed in extracts of rat hearts perfused in vitro with media containing glucose and insulin+/-acetate+/-dichloroacetate. Dichloroacetate (100mum, 1mm or 10mm) increased the activity of pyruvate dehydrogenase in perfusions with glucose or glucose+acetate. Evidence is given that dichloroacetate may facilitate the conversion of pyruvate dehydrogenase from an inactive (phosphorylated) form into an active (dephosphorylated) form.
authors.affiliation: Department of Biochemistry, Medical School, University of Bristol, University Walk, Bristol BS8 1TD, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Whitehouse
authors.forename: P J
authors.initials: PJ
authors.lastname: Randle