Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells: in vitro analysis.
OBJECTIVE
To analyze, in vitro, the effects of acetylsalicylic acid (aspirin) and acetic acid solutions on VX2 carcinoma cells in suspension and to examine the correlation between these effects and neoplastic cell death.


METHODS
The VX2 tumor cells (10(7) cells/ml) were incubated in solutions containing differing concentrations (2.5% and 5%) of either acetylsalicylic acid or acetic acid, or in saline solution (controls). Every five minutes, cell viability was tested (using the trypan blue test) and analyzed under light microscopy.


RESULTS
Tumor cell viability (in %) decreased progressively and, by 30 minutes, neoplastic cell death had occurred in all solutions.


CONCLUSIONS
Based on this experimental model and the methodology employed, we conclude that these solutions cause neoplastic cell death in vitro.
Saad-Hossne
Rogério
R
Department of Surgery and Orthopedics, Botucatu Medical School, Paulista State University, Brazil. saad@fmb.unesp.br
Prado
René Gamberini
RG
Hossne
William Saad
WS
D019342:Acetic Acid / Q000627:therapeutic use*; D000818:Animals; D001241:Aspirin / Q000627:therapeutic use*; D016923:Cell Death; D002470:Cell Survival / Q000187:drug effects; D004338:Drug Combinations; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D066298:In Vitro Techniques; D007202:Indicators and Reagents / Q000627:therapeutic use*; D008856:Microscopy, Fluorescence; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D011817:Rabbits
D004338:Drug Combinations; D007202:Indicators and Reagents; D019342:Acetic Acid; D001241:Aspirin