title: The repeatability and effect of season on seminal characteristics and computer-aided sperm analysis in the stallion.
abstract: The within-stallion repeatability and effect of season on sperm movement characteristics, determined by computer-aided sperm analysis (CASA), were compared with those of other seminal characteristics. The computer-aided determinations of sperm movement were more repeatable than the seminal characteristics of gel-free volume and sperm cell concentration based on coefficients of variation obtained from the analysis of multiple ejaculates from the same stallions. A significant (P<0.05) seasonal effect on the computer-aided movement characteristic of mean sperm linearity was observed, with a reduction in sperm linearity in the winter months. The percentage of motile and progressively motile cells and mean sperm velocity determined by CASA also tended to be lower in the winter months. These changes in sperm movement characteristics paralleled changes in other seminal characteristics. The use of CASA may have value in the potential fertility evaluation of a stallion in that it can provide relatively precise quantification of sperm movement characteristics from the evaluation of only a few ejaculates. Some CASA derived sperm movement characteristics may be lower during the physiological nonbreeding season than during the breeding season.
authors.affiliation: New York State College of Veterinary Medicine Cornell University, Ithaca, NY 14853 USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Jasko
authors.forename: D H
authors.initials: DH
authors.lastname: Lein
authors.forename: R H
authors.initials: RH
authors.lastname: Foote