title: Digestion of DNA regions to discriminate ochratoxigenic and non-ochratoxigenic strains in the Aspergillus niger aggregate.
abstract: Aspergillus strains belonging to the Aspergillus niger aggregate, either isolated from Italian grapes or received from public collections, were analysed in order to discriminate between the ochratoxin A (OTA) producing and the non-producing strains by means of the analysis of Internal Transcribed Spacers (ITS), Intergenic Spacers (IGS) and of a beta-tubulin gene portion. A. niger and Aspergillus awamori were identified observing the macro- and microscopic features of the colonies and the strains ochratoxigenicity was evaluated through Thin Layer Chromatography and/or High Performance Liquid Chromatography. PCR amplification of ITS, IGS and beta-tubulin gene portion produced 600, 440 and 550 bp amplicons, respectively, in all the analysed strains. The digestion of the IGS amplicon with Hinf I and of the other two amplicons with Rsa I, revealed one main profile type ("T-C-C") associated with the A. niger aggregate strains which lack the ability to produce OTA.
authors.affiliation: C.R.A.-Istituto Sperimentale per la Viticoltura, Viale XXVIII aprile, 26-31015 Conegliano (TV), Italy. alessandro.zanzotto@ispervit.it
authors.forename: A
authors.initials: A
authors.lastname: Zanzotto
authors.forename: S
authors.initials: S
authors.lastname: Burruano
authors.forename: P
authors.initials: P
authors.lastname: Marciano
mesh_terms: D001234:Aspergillus niger / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004271:DNA, Fungal / Q000032:analysis*; D021901:DNA, Intergenic; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005784:Gene Amplification; D008970:Molecular Weight; D009793:Ochratoxins / Q000032:analysis / Q000096:biosynthesis*; D014404:Tubulin / Q000032:analysis; D027843:Vitis / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic; D009793:Ochratoxins; D014404:Tubulin; C025589:ochratoxin A