Potential use of glycogen level as biomarker of chemical stress in Biomphalaria glabrata.
Biomphalaria glabrata, a freshwater gastropod mollusc, was tested as biondicator organism to assess cadmium, lead and arsenic exposure using acute laboratory bioassays. Modifications of glycogen levels were measured in different anatomical regions of B. glabrata in order to test the usefulness of this parameter as a general biomarker of chemical stress. The snails were exposed 96 h to different concentrations of the following contaminants: 0.1 and 0.05 mg Cd/L; 0.5, 0.1 and 0.05 mg Pb/L; 0.5, 0.1 and 0.05 mg As/L. Significant decreases in the polysaccharide content were observed in gonadal region for all treated animals. Arsenic and lead at 0.1 and 0.5 mg/L level of exposure were also able to decrease the levels of glycogen in the pulmonary and digestive gland region. Glycogen content in the cephalopedal region of treated animals presented a significant decrease (p<0.05) when compared with control organisms only for arsenic at the highest level of exposure. To establish possible correlations between glycogen and contaminants accumulated by snails, analyses of the elements bioaccumulated in the different anatomical regions of B. glabrata were also performed. Cadmium and lead followed a similar pattern of bioaccumulation with highest values in the digestive gland region. Arsenic bioaccumulation, however, was highest in the gonadal region.
Ansaldo
Martín
M
Instituto Antártico Argentino, Cerrito 1248, 1010 Buenos Aires, Argentina. tincho@qb.fcen.uba.ar
Nahabedian
Daniel E
DE
Holmes-Brown
Eduardo
E
Agote
Marcos
M
Ansay
Cristina V
CV
Guerrero
Noemí R Verrengia
NR
Wider
Eva A
EA
D000818:Animals; D001151:Arsenic / Q000493:pharmacokinetics / Q000633:toxicity; D015415:Biomarkers; D001700:Biomphalaria / Q000378:metabolism*; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity; D006003:Glycogen / Q000378:metabolism*; D007854:Lead / Q000493:pharmacokinetics / Q000633:toxicity; D008670:Metals / Q000378:metabolism / Q000633:toxicity; D009942:Organometallic Compounds / Q000633:toxicity; D013312:Stress, Physiological; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D008670:Metals; D009942:Organometallic Compounds; D014874:Water Pollutants, Chemical; D002104:Cadmium; D007854:Lead; D006003:Glycogen; D001151:Arsenic