[Studies on polyploid induction of Phellodendron chinense].
OBJECTIVE
This paper aimed at improving existing breeds of Phellodendron chinense and enriching the resources of heredity breeding.


METHODS
The polyploid of P. chinense was obtained using calli, seeds and buds, which were treated with colchicines solution.


RESULTS
The polyploid of P. chinense was obtained using calli with stem treated with 0.2% colchicines medium, the inducing rate reached 12.45%. Compared with the morphology and chromosomal number the polyploid induction was proved to be effective.


CONCLUSIONS
It was an effective biotechnological way to use colchicines to induce polyploid on the different explants of P. chinense.
Wang
Yue-Hua
YH
Department of Biological Engineering, Chengdu University, Chengdu 610160, China. tlfor@hotmail.com
D032461:Chromosomes, Plant / Q000235:genetics*; D003078:Colchicine / Q000494:pharmacology*; D032102:Phellodendron / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D018520:Plant Shoots / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D011123:Polyploidy; D012639:Seeds / Q000235:genetics / Q000254:growth & development
D003078:Colchicine