An intimate collaboration between peroxisomes and lipid bodies.
Although peroxisomes oxidize lipids, the metabolism of lipid bodies and peroxisomes is thought to be largely uncoupled from one another. In this study, using oleic acid-cultured Saccharomyces cerevisiae as a model system, we provide evidence that lipid bodies and peroxisomes have a close physiological relationship. Peroxisomes adhere stably to lipid bodies, and they can even extend processes into lipid body cores. Biochemical experiments and proteomic analysis of the purified lipid bodies suggest that these processes are limited to enzymes of fatty acid beta oxidation. Peroxisomes that are unable to oxidize fatty acids promote novel structures within lipid bodies ("gnarls"), which may be organized arrays of accumulated free fatty acids. However, gnarls are suppressed, and fatty acids are not accumulated in the absence of peroxisomal membranes. Our results suggest that the extensive physical contact between peroxisomes and lipid bodies promotes the coupling of lipolysis within lipid bodies with peroxisomal fatty acid oxidation.
Binns
Derk
D
Department of Pharmacology, University of Texas Southwestern Medical School, Dallas, TX 75390, USA.
Januszewski
Tom
T
Chen
Yue
Y
Hill
Justin
J
Markin
Vladislav S
VS
Zhao
Yingming
Y
Gilpin
Christopher
C
Chapman
Kent D
KD
Anderson
Richard G W
RG
Goodman
Joel M
JM
D042962:Acyl-CoA Oxidase / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D022083:Cytoplasmic Structures / Q000378:metabolism* / Q000648:ultrastructure; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000378:metabolism; D008954:Models, Biological; D015394:Molecular Structure; D019301:Oleic Acid / Q000494:pharmacology; D000074435:Peroxisome-Targeting Signal 1 Receptor; D020675:Peroxisomes / Q000378:metabolism* / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013997:Time Factors
D005227:Fatty Acids; D008055:Lipids; D026901:Membrane Transport Proteins; C499509:PEX5 protein, S cerevisiae; D000074435:Peroxisome-Targeting Signal 1 Receptor; D029701:Saccharomyces cerevisiae Proteins; D019301:Oleic Acid; D042962:Acyl-CoA Oxidase; C467991:POX1 protein, S cerevisiae