title: Effect of cyclic AMP on fructose utilization, progressive motility and protein synthesis by ram spermatozoa.
abstract: Ejaculated washed ram spermatozoa showed consistent increases in the intracellular concentration of cyclic 3', 5'-adenosine monophosphate (cAMP) after incubation for 15 minutes with the phosphodiesterase (PDE)-inhibitors, theophylline and caffeine. In vitro addition of cAMP or PDE-inhibitors to ram semen also stimulated and maintained sperm motility and enhanced the rate of fructose utilization. The same doses of cAMP or theophylline significantly stimulated the rate of protein synthesis by the washed spermatozoa, while the PDE-stimulator, imidazole, inhibited protein synthesis significantly. The stimulatory effect of cAMP on sperm protein synthesis was not affected by cycloheximide, but was abolished by the mitochondrial inhibitor, chloramphenicol. The present results indicate a positive correlation between the intracellular concentration of cAMP and the rates of progressive motility, fructose utilization, and protein synthesis by ram spermatozoa. The results suggest that the effect of cAMP is associated with the synthesis of mitochndrial proteins which may be involved with the observed enhancement of sperm motility and metabolism. The data also indicate that cAMP map act either as a first or a second messenger in mature spermatoza.
authors.affiliation: Department of Animal Production, Faculty of Agriculture Alexandria University, Alexandria, Egypt.
authors.forename: M H
authors.initials: MH
authors.lastname: Salem
authors.forename: M Y
authors.initials: MY
authors.lastname: Mekkawy
authors.forename: N A
authors.initials: NA
authors.lastname: Ahmed
authors.forename: I Y
authors.initials: IY
authors.lastname: Abdel-Aziz
authors.forename: A A
authors.initials: AA
authors.lastname: Mohamed
authors.forename: H A
authors.initials: HA
authors.lastname: El-Oksh
authors.forename: V G
authors.initials: VG
authors.lastname: Pursel