IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry.
Most MALDI instrumentation uses UV lasers. We have designed a MALDI-IM-oTOF-MS which employs both a Nd:YAG laser pumped optical parametric oscillator (OPOTEK, lambda = 2.8-3.2 microm at 20 Hz) to perform IR-LDI or IR-MALDI and a Nd:YLF laser (Crystalaser, lambda = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives a fast separation and analysis of biomolecules from complex mixtures in which ions of similar chemical type fall along well-defined "trend lines". Our data shows that ion mobility allows multiply charged monomers and multimers to be resolved; thus, yielding pure spectra of the singly charged protein ion which are virtually devoid of chemical noise. In addition, we have demonstrated that IR-LDI produced similar results as IR-MALDI for the direct tissue analysis of phospholipids from rat brain.
Woods
Amina S
AS
NIDA IRP, NIH, Baltimore, Maryland 21224, USA. awoods@intra.nida.nih.gov
Ugarov
Michael
M
Jackson
Shelley N
SN
Egan
Thomas
T
Wang
Hay-Yan J
HY
Murray
Kermit K
KK
Schultz
J Albert
JA
D000818:Animals; D001921:Brain / Q000378:metabolism; D002642:Chick Embryo; D045304:Cytochromes c / Q000032:analysis; D006736:Horses; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D009113:Muramidase / Q000032:analysis; D009206:Myocardium / Q000201:enzymology; D010743:Phospholipids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014466:Ultraviolet Rays
D010743:Phospholipids; D011506:Proteins; D045304:Cytochromes c; D009113:Muramidase