title: [Cloning and characterization of two novel alternatively spliced transcripts of ZNF268].
abstract: ZNF268 gene encodes a typical KRAB-containing zinc finger protein, which has an amino-terminal KRAB domain and 24 carboxyl-terminal C2H2 zinc finger motifs. There is evidence that ZNF268 is expressed in human early embryos, and that it played a role in the development of human fetal liver. In this study, we use immunohistochemistry to show that ZNF268 was also expressed in hematopoietic stem cells in 3-5 week-old human embryos. ZNF268 expression was also detected in the blood cells from 7 healthy adult donors by RT-PCR. Furthermore, 4 alternative transcripts were detected in the 7 samples. By cloning and sequencing, two novel transcripts, ZNF268c and ZNF268d were identified. We predict that there is association between ZNF268 and development of hematopoietic cells.
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan 430072, China. lunnarnight@163.com
authors.forename: Chong
authors.initials: C
authors.lastname: Sun
authors.forename: Zhou-Zhou
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Huan-Jie
authors.initials: HJ
authors.lastname: Shao
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Li
mesh_terms: D017398:Alternative Splicing; D001773:Blood Cells / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D008297:Male; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C482295:ZNF268 protein, human