title: Nesterenkonia aethiopica sp. nov., an alkaliphilic, moderate halophile isolated from an Ethiopian soda lake.
abstract: Strain DSM 17733(T), isolated from the shore of Lake Abjata in Ethiopia, is a heterotrophic, alkaliphilic, moderately halophilic, Gram-positive, strictly aerobic, non-motile,non-endospore-forming bacterium. The organism grows optimally at 30-37 degrees C, pH 9 and 3 % (w/v) NaCl. Analysis of the cell wall showed the presence of murein of the type L-lys-gly-L-Glu, variation A4alpha. The G + C content of the genomic DNA was 69.0 mol%. Sequence analysis of 16S rRNA gene sequence of strain DSM 17733(T) placed the isolate in the genus Nesterenkonia. DNA-DNA hybridization of DSM 17733(T) with those organisms with the closest phylogenetic affiliation, i.e. Nesterenkonia halobia, Nesterenkonia lacusekhoensis and Nesterenkonia xinjiangensis, gave relatedness values of 48.5 %, 63.7 % (repetition, 57.2 %) and 35.7 % (repetition, 29.3 %), respectively. On the basis of both phenotypic and phylogenetic criteria and the low levels of DNA-DNA relatedness with the phylogenetically closest species N. xinjiangensis and N. halobia, it is proposed that the isolate be classified in a novel species, Nesterenkonia aethiopica sp. nov. The type strain is DSM 17733(T) (=CCUG 48939(T)).
authors.affiliation: Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Delgado
authors.affiliation: Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
authors.forename: Jorge
authors.initials: J
authors.lastname: Quillaguamán
authors.affiliation: Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Bakhtiar
authors.affiliation: Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
authors.affiliation: Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
authors.forename: Amare
authors.initials: A
authors.lastname: Gessesse
authors.affiliation: Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
authors.forename: Rajni
authors.initials: R
authors.lastname: Hatti-Kaul
mesh_terms: D005002:Ethiopia; D005618:Fresh Water / Q000382:microbiology; D006094:Gram-Positive Bacteria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S