title: Cutting edge: impaired transitional B cell production and selection in the nonobese diabetic mouse.
abstract: Developing B cells undergo selection at multiple checkpoints to eliminate autoreactive clones. We analyzed B cell kinetics in the NOD mouse to establish whether these checkpoints are intact. Our results show that although bone marrow production is normal in NOD mice, transitional (TR) B cell production collapses at 3 wk of age, reflecting a lack of successful immature B cell migration to the periphery. This yields delayed establishment of the follicular pool and a lack of selection at the TR checkpoint, such that virtually all immature B cells that exit the bone marrow mature without further selection. These findings suggest that compromised TR B cell generation in NOD mice yields relaxed TR selection, affording autoreactive specificities access to mature pools.
authors.affiliation: University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Quinn
authors.forename: Negin
authors.initials: N
authors.lastname: Noorchashm
authors.forename: Jenni E
authors.initials: JE
authors.lastname: Crowley
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Reed
authors.forename: Hooman
authors.initials: H
authors.lastname: Noorchashm
authors.forename: Ali
authors.initials: A
authors.lastname: Naji
authors.forename: Michael P
authors.initials: MP
authors.lastname: Cancro
mesh_terms: D000375:Aging / Q000235:genetics / Q000276:immunology; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology / Q000473:pathology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D002453:Cell Cycle / Q000235:genetics / Q000276:immunology*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D019070:Cell Lineage / Q000235:genetics / Q000276:immunology; D002464:Cell Migration Inhibition; D002999:Clone Cells; D020022:Genetic Predisposition to Disease; D018655:Lymphocyte Count; D008231:Lymphopenia / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D013045:Species Specificity; D013234:Stem Cells / Q000166:cytology / Q000276:immunology