title: A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays.
abstract: We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatibility with standard methods for protein assays. This feature greatly expands the range of applications for assay systems quantifying the expression of multiple luciferase reporters.
authors.affiliation: Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Hampf
authors.forename: Manfred
authors.initials: M
authors.lastname: Gossen
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D048888:Fireflies / Q000201:enzymology / Q000235:genetics; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D049409:Luciferases, Firefly / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000235:genetics; D049410:Luciferases, Renilla / Q000032:analysis* / Q000235:genetics; D008163:Luminescent Measurements / Q000379:methods; D048790:Renilla / Q000201:enzymology / Q000235:genetics
chemical_list: D004791:Enzyme Inhibitors; C485197:luciferase, Photinus; D049410:Luciferases, Renilla; D049409:Luciferases, Firefly