title: Surfactin C inhibits platelet aggregation.
abstract: This study was designed to investigate the effect of surfactin C, which is derived from Bacillus subtilis, on platelet aggregation and homotypic leucocyte aggregation. Surfactin C strongly and dose-dependently inhibited platelet aggregation, which was stimulated both by thrombin (0.1 U mL(-1)), a potent agonist that activates the G protein-coupled protease receptor, and by collagen (5 microg mL(-1)), a potent ligand that activates alpha(IIb)beta(3) with IC50 values (concentration inhibiting platelet aggregation by 50%) of 10.9 and 17.0 microM, respectively. Moreover, surfactin C significantly suppressed the intracellular Ca(2+) mobilization in thrombin-activated platelets. Surfactin C, however, did not affect various integrin-mediated U937 cell aggregation, implying that the anti-platelet activity of surfactin C was not due to its detergent effect but by its action on the downstream signalling pathway. Therefore, the results suggest that surfactin C may have a beneficial therapeutic effect on aberrant platelet aggregation-mediated cardiovascular diseases.
authors.affiliation: Laboratory of Veterinary Physiology & Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Sung Dae
authors.initials: SD
authors.lastname: Kim
authors.forename: Sun Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Jae Youl
authors.initials: JY
authors.lastname: Cho
authors.forename: Hwa Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Jong Hwan
authors.initials: JH
authors.lastname: Lim
authors.forename: Hyo In
authors.initials: HI
authors.lastname: Yun
authors.forename: Seung Chun
authors.initials: SC
authors.lastname: Park
authors.forename: Kyoung Youl
authors.initials: KY
authors.lastname: Lee
authors.forename: Sang Keun
authors.initials: SK
authors.lastname: Kim
authors.forename: Man Hee
authors.initials: MH
authors.lastname: Rhee
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D055666:Lipopeptides; D010456:Peptides, Cyclic / Q000494:pharmacology*; D010741:Phospholipases A / Q000502:physiology; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D013917:Thrombin / Q000494:pharmacology
chemical_list: D055666:Lipopeptides; D010456:Peptides, Cyclic; D010975:Platelet Aggregation Inhibitors; C009365:surfactin peptide; D003094:Collagen; D010741:Phospholipases A; D013917:Thrombin; D002118:Calcium