Molecular effect of FadD on the regulation and metabolism of fatty acid in Escherichia coli.
Wild-type Escherichia coli can utilize exogenous long-chain fatty acids (C>12) as a sole carbon source for growth, but it cannot grow on medium-chain fatty acids. However, when the fadD gene from E. coli MG1655 was expressed in E. coli DH5alpha, the latter strain was found to utilize medium-chain fatty acids as its sole carbon source. Various E. coli strains thought to carry subtly different FadR phenotypes were all confirmed as using medium-chain fatty acids as their sole carbon source when the additional fadD was expressed. Semi-quantitative reverse transcription PCR analysis showed that the additional expression of fadD in E. coli strongly induced the expression of both fadE and fadBA. Excessive amounts of medium-chain acyl-CoA would probably bind to FadR and remove the inhibition of fatty acid beta-oxidation.
Zhang
Hanxing
H
State Key Laboratory of Microbial Technology, Life Science School, Shandong University, Jinan, China.
Wang
Peng
P
Qi
Qingsheng
Q
D001483:Base Sequence; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D010084:Oxidation-Reduction; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D005227:Fatty Acids; D003066:Coenzyme A Ligases; C085934:acyl-coenzyme A synthetase, E coli