[Study on induction of polyploidy in Salvia bowleyana by colchicine treatment].
OBJECTIVE
To explore the technique of induction of polyploidy in Salvia bowleyana by colchicine treatment.


METHODS
The three kinds of explant of bud, leaf and calli were induced by colchicine treatment.


RESULTS
The induction effects were better when the calli was treated by colchicines (15 mg x L(-1)) and the leaf was pre-cultured for one week. The doubling rate was 33.33%, while the majority were wholy doubled plants, and the leaves were thicker and broader, the color was darker, the root was thicker and the stoma size was obviously bigger than the diploid plants. The number of chromosome were 8 to 64. Isoenzyme analysis showed that the enzyme activities between the polyploid and the diploid plants were quite different.


CONCLUSIONS
Induction of polyploidy by colchicine treatment is efficacious. The part of the doubled plants were identified as homologmous tetraploids.
Duan
Ying-Zi
YZ
Department of Biology, Tangshan Teacher's College, Tangshan 063000, China. dyz03247@sohu.com
Ke
Shao-Ying
SY
Cao
Jing
J
Niu
Ying-Ze
YZ
Peng
Chao-Zhong
CZ
D032461:Chromosomes, Plant / Q000235:genetics*; D003078:Colchicine / Q000494:pharmacology*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D018520:Plant Shoots / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D011123:Polyploidy; D027544:Salvia / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development
D003078:Colchicine