Isotachophoresis in free-flow using a miniaturized device.
For the first time, we report a miniaturized approach for isotachophoresis employing the technique of free-flow electrophoresis. Using a micromachined separation chamber with a volume of 200 nL, a sample mixture of fluorescein, eosin G, and acetylsalicylic acid was separated, stacked, and concentrated in less than a minute. Additionally, an isotachophoretic separation of a reaction mixture of myoglobin and fluoresceinisothiocyanate as a fluorescence label has shown the potential of this method for on-line sample preparation.
Janasek
Dirk
D
Institute for Analytical Sciences Dortmund and Berlin, Bunsen-Kirchhoff-Strasse 11, D-44139 Dortmund, Germany. d.janasek@ansci.de
Schilling
Michael
M
Franzke
Joachim
J
Manz
Andreas
A