[Biological significance of E-cadherin in an inflammatory breast carcinoma cell line].
OBJECTIVE
To investigate the effects of E-cadherin on the biologic behavior of SUM149, an inflammatory breast cancer cell line.


METHODS
SUM149 cells were transfected with dominant-negative mutant E-cadherin expressing plasmid. The positive clones with higher expression of dominant-negative E-cadherin mutant were identified by RT-PCR and fluorescent flow cytometry method. The differences in cell growth, proliferation and invasion between positive clones and controls were compared.


RESULTS
Whereas the proliferation of positive clones was of no change, compared with controls, the ability of invasion was decreased and the mRNA levels of MMP-1 and MMP-9 were downregulated. Gelatin zymography analysis also confirmed the decreasing expression of MMP-9 in the positive clones.


CONCLUSIONS
In this cell line model, down-regulation of E-cadherin can inhibit the ability of invasion of this inflammatory breast cancer cell line.
Dong
Hui-ming
HM
Department of Breast Surgery, Cancer Institute, Shanghai Cancer Hospital, Fudan University, Shanghai 200032, China.
Liu
Gang
G
Wu
Jiong
J
Lu
Jin-song
JS
Luo
Jian-min
JM
Shen
Zhen-zhou
ZZ
Shao
Zhi-min
ZM
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D009154:Mutation; D009361:Neoplasm Invasiveness; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D015820:Cadherins; D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1