QTL analysis.
The study of the intraspecific natural variation existing in Arabidopsis provides a useful resource for the dissection of its genome at the functional, ecological, and evolutionary levels. A major step in these studies is the identification and location of quantitative trait loci (QTLs) determining the variation for trait(s) of interest, so-called QTL analysis. Here we provide general methods to achieve this goal, including (1) the selection of wild accessions that can serve as parental lines for QTL mapping; (2) the development of mapping populations; (3) the construction of the required genome-wide linkage maps of the populations; and (4) the mapping and characterization of QTLs affecting the trait(s).
Alonso-Blanco
Carlos
C
Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Centro Nacional de Biotecnologiá (CNB), CSIC, Cantoblanco, Madrid, Spain.
Koornneef
Maarten
M
van Ooijen
Johan W
JW
D017360:Arabidopsis / Q000235:genetics*; D004843:Epistasis, Genetic; D017343:Genes, Plant; D008040:Genetic Linkage; D005821:Genetic Techniques; D014644:Genetic Variation; D018745:Genome, Plant; D008126:Lod Score; D008957:Models, Genetic; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D011995:Recombination, Genetic