[Effect of 2, 5-hexanedione on calcium homeostasis of motor neuron].
OBJECTIVE
To explore the mechanism of cytotoxic effect of 2, 5-hexanedione (2, 5-HD) on motor neuron.


METHODS
Vsc4.1 (a cell line from motor neuron) was incubated with a series concentration of 2, 5-HD. The cell viability, Ca(2+) Mg(2+) ATPase and Na(+)K(+) ATPase were detected. Laser scanning confocal microscope (LSCM) was used for detecting intracellular calcium level. The average calcium level in VSC4.1 was measured by flow cytometry.


RESULTS
The cell viability was decreased when Vsc4.1 cells were treated with 2, 5-HD at the dosage of 2.5, 5.0, 7.5, 10, 15 and 20 mmol/L for 24 hours. Compared with the control group the activity of Ca(2+) Mg(2+) ATPase was decreased to 70.02%, 77.44% and 47.47% respectively; the activity of Na(+)K(+) ATPase was decreased to 82.07%, 72.45% and 50.71%. The difference was significant. Intracellular free calcium of VSC4.1 cell was increased rapidly within 10 s and then recovered within 40 seconds when it was exposed to 33.5 mmol/L 2, 5-HD. An increase in intracellular calcium was observed when the VSC4.1 was treated with 33.5 mmol/L 2, 5-HD. The peak of intracellular calcium level occurred ten minutes later.


CONCLUSIONS
The disturbance of calcium homeostasis may be involved in the mechanisms of neurotoxicity of 2, 5-HD.
Zhang
Yan-shu
YS
National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, PR China, 100050.
Liu
Qiang
Q
Liu
Qing-jun
QJ
Duan
Hua-wei
HW
He
Feng-sheng
FS
Zheng
Yu-xin
YX
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006588:Hexanones / Q000633:toxicity*; D009046:Motor Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D006588:Hexanones; C011269:2,5-hexanedione; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium