Bioanalytical interaction studies executed by preincubation affinity capillary electrophoresis.
The versatility of CE is beneficial for the study of many types of molecular interactions, because different experimental designs can be made to suit the characteristics of a particular interaction. A very versatile starting point is the preequilibration type of affinity CE that has been used extensively for characterizing biomolecular interactions in the last 15 years. We review this field here and include a comprehensive overview of the existing preincubation ACE modes including their advantages and limitations as well as the methodological developments and applications within the bioanalytical field.
Ostergaard
Jesper
J
Department of Pharmaceutics and Analytical Chemistry, The Danish University of Pharmaceutical Sciences, Copenhagen, Denmark. joe@dfuni.dk
Heegaard
Niels H H
NH
D000818:Animals; D000937:Antigen-Antibody Reactions; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D004347:Drug Interactions; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009696:Nucleic Acids / Q000032:analysis / Q000378:metabolism; D025941:Protein Interaction Mapping; D011506:Proteins / Q000032:analysis / Q000378:metabolism
D002241:Carbohydrates; D009696:Nucleic Acids; D011506:Proteins