[Protective effect of puerarin on stress-induced gastric mucosal injury in rats].
OBJECTIVE
To study the protective effect of puerarin on stress-induced gastric mucosal injury in rats.


METHODS
The model of gastric ulcer was established by restraint plus water-immersion stress in rats. Gastric motility was monitored by the method of "Gas Balloon". Gastric mucosal blood flow was recorded by laser-Doppler flowmetry. Colorimetric method was used to determine the content of NO and ET in gastric mucosal tissue. Meantime the pathologic changes of gastric mucosal was examined.


RESULTS
Puerarin could significantly attenuated gastric mucosal damage induced by water-immersion stress, inhibited gastric motility, specially decreased the index of gastric motility and percentage of gastric contraction time and numbers of violent contraction. The gastric mucosal blood flow and NO level in gastric mucosal were enhanced, while ET level was reduced by puerarin. The degree of tissue damage in gastric mucosal was also significantly attenuated after administration fo puerarin.


CONCLUSIONS
Puerarin exerts a significant protective effect on water-immersion stress-induced gastric mucosal damage by relaxing the vessels, increasing NO level in gastric mucosal, increasing regional gastric mucosal blood flow and inhibiting gastric motility.
Wang
Fu-Wen
FW
Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062, China. wangfuwww@tom.com
Li
Jie
J
Hu
Zhi-Li
ZL
Xie
Yan-Ying
YY
D000818:Animals; D016232:Endothelins / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D005769:Gastrointestinal Motility / Q000187:drug effects; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D029908:Pueraria / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000187:drug effects; D013276:Stomach Ulcer / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D013312:Stress, Physiological / Q000150:complications*
D016232:Endothelins; D007529:Isoflavones; D020011:Protective Agents; D009569:Nitric Oxide; C033607:puerarin