14.6 mT ELF magnetic field exposure yields no DNA breaks in model system Salmonella, but provides evidence of heat stress protection.
In this study, we demonstrate that common extremely low frequency magnetic field (MF) exposure does not cause DNA breaks in this Salmonella test system. The data does, however, provide evidence that MF exposure induces protection from heat stress. Bacterial cultures were exposed to MF (14.6 mT 60 Hz field, cycled 5 min on, 10 min off for 4 h) and a temperature-matched control. Double- and single-stranded DNA breaks were assayed using a recombination event counter. After MF or control exposure they were grown on indicator plates from which recombination events can be quantified and the frequency of DNA strand breaks deduced. The effect of MF was also monitored using a recombination-deficient mutant (recA). The results showed no significant increase in recombination events and strand breaks due to MF. Evidence of heat stress protection was determined using a cell viability assay that compared the survival rates of MF exposed and control cells after the administration of a 10 min 53 degrees C heat stress. The control cells exhibited nine times more cell mortality than the MF exposed cells. This Salmonella system provides many mutants and genetic tools for further investigation of this phenomenon.
Williams
Parley A
PA
Department of Physics, University of Utah, Salt Lake City, Utah 84112, USA.
Ingebretsen
Richard J
RJ
Dawson
Renee J
RJ
D004249:DNA Damage; D004269:DNA, Bacterial / Q000528:radiation effects*; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006358:Hot Temperature / Q000009:adverse effects*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000528:radiation effects*
D004269:DNA, Bacterial