Overproduction of wild-type and bioengineered derivatives of the lantibiotic lacticin 3147.
Lacticin 3147 is a broad-spectrum two-peptide lantibiotic whose genetic determinants are located on two divergent operons on the lactococcal plasmid pMRC01. Here we introduce each of 14 subclones, containing different combinations of lacticin 3147 genes, into MG1363 (pMRC01) and determine that a number of them can facilitate overproduction of the lantibiotic. Based on these studies it is apparent that while the provision of additional copies of genes encoding the biosynthetic/production machinery and the regulator LtnR is a requirement for high-level overproduction, the presence of additional copies of the structural genes (i.e., ltnA1A2) is not.
Cotter
Paul D
PD
Department of Microbiology, University College Cork, Cork, Ireland.
Draper
Lorraine A
LA
Lawton
Elaine M
EM
McAuliffe
Olivia
O
Hill
Colin
C
Ross
R Paul
RP
D001426:Bacterial Proteins / Q000096:biosynthesis; D001430:Bacteriocins / Q000096:biosynthesis*; D001483:Base Sequence; D001698:Biomedical Engineering / Q000379:methods*; D017931:DNA Primers; D017045:Lactococcus / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D001430:Bacteriocins; D017931:DNA Primers; C082508:lacticin 481