title: [Human rhinovirus detection from infants and young children with acute respiratory infections by nested-polymerase chain reaction].
abstract: OBJECTIVE
  To develop a rapid, sensitive and specific method for detection human rhinovirus (HRV) from clinical specimens.
  
  
  METHODS
  Primers derived from the highly conserved 5'noncoding region of human rhinovirus were used to develop a nested RT-PCR for detecting HRV. The sensitivity and specificity of the RT-PCR were determined using various RNA while DNA viruses were used as control. Seven hundred and seventy-one specimens collected from children with symptoms of acute respiratory infections from Nov. 2002 to Oct. 2003 were analyzed for HRV by RT-PCR as well as for other respiratory viruses through isolation of virus and indirect immunofluorescent assay.
  
  
  RESULTS
  Only the cDNA from HRV was positive by RT-PCR, indicating the nested RT-PCR was specific. With RT-PCR, HRV were detected in 148 out of 771 specimens (19.2%). As for HRV positive rates, it was found 53.3% in pharyngitis patients; 43.8% in laryngitis patients and 28.7% in bronchitis patients. In Sep. 2002 and from Aug. 2003 to Oct. 2003, HRV positive rates were high (21.6% - 32.6%), with Sep. 2003 in particular--32.6%. From Mar. 2003 to Jul. 2003, HRV positive rates maintained from 16.0% to 19.1%.
  
  
  CONCLUSIONS
  HRV was one of the important agents for acute respiratory infections in infants and young children in Beijing.
authors.affiliation: Laboratory of Virology, Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China.
authors.forename: Lin-qing
authors.initials: LQ
authors.lastname: Zhao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qian
authors.forename: Ru-nan
authors.initials: RN
authors.lastname: Zhu
authors.forename: Jie
authors.initials: J
authors.lastname: Deng
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
mesh_terms: D020121:5' Untranslated Regions; D000208:Acute Disease; D002675:Child, Preschool; D017124:Conserved Sequence; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007223:Infant; D012367:RNA, Viral / Q000032:analysis; D012141:Respiratory Tract Infections / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012229:Rhinovirus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D020121:5' Untranslated Regions; D004279:DNA, Viral; D012367:RNA, Viral