title: A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis.
abstract: A simple and universally applicable protocol for extracting high-quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysis.
authors.affiliation: Dipartimento di Scienze Ambientali G. Sarfatti,Università di Siena, Italy. mails_wang@yahoo.com
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Rita
authors.initials: R
authors.lastname: Vignani
authors.forename: Monica
authors.initials: M
authors.lastname: Scali
authors.forename: Mauro
authors.initials: M
authors.lastname: Cresti
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D010940:Plant Proteins / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry*; D040901:Proteomics; D016622:Silver Staining
chemical_list: D010940:Plant Proteins