cDNA cloning and sequence analysis of preproendothelin-1 (PPET-1) from salmon, Oncorhynchus keta.
The presence of endothelin (ET)-like immunoreactivity and the cardiovascular effects of mammalian ET-1 in fish have been reported. To identify ET-related peptides in fish, we screened the cDNA library of the salmon (Oncorhynchus keta) stomach by means of rapid amplification of cDNA ends, and we cloned cDNAs encoding an ET-related peptide. The salmon ET-related sequence of 21 amino acids is identical to the trout ET-1 peptide recently purified from kidney specimens of Oncorhynchus mykiss. The deduced amino acid sequence of salmon pre-proET-1 (PPET-1) comprises 244 amino acids, including a putative signal sequence and mature ET-1, as well as big ET-1 and ET-1-like sequences. This precursor, the first reported PPET-1 sequence for Salmoniformes, Teleostei, has low homology with the sequences of human, mouse, frog (Xenopus laevis), and zebrafish (Danio rerio) PPET-1 (26%, 29%, 24%, and 39%, respectively).
Wang
Hongyu
H
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566 Japan.
Quan
Jiexia
J
Kotake-Nara
Ei-Ichi
E
Uchide
Tsuyoshi
T
Andoh
Tadashi
T
Saida
Kaname
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D019332:Endothelin-1 / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D017685:Oncorhynchus keta / Q000235:genetics / Q000378:metabolism*; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics*; D017421:Sequence Analysis
D018076:DNA, Complementary; D019332:Endothelin-1; D011498:Protein Precursors; C093240:proendothelin 1