title: Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex.
abstract: The RNA replication complexes of mammalian positive-stranded RNA viruses are generally associated with (modified) intracellular membranes, a feature thought to be important for creating an environment suitable for viral RNA synthesis, recruitment of host components, and possibly evasion of host defense mechanisms. Here, using a panel of replicase-specific antisera, we have analyzed the earlier stages of severe acute respiratory syndrome coronavirus (SARS-CoV) infection in Vero E6 cells, in particular focusing on the subcellular localization of the replicase and the ultrastructure of the associated membranes. Confocal immunofluorescence microscopy demonstrated the colocalization, throughout infection, of replicase cleavage products containing different key enzymes for SARS-CoV replication. Electron microscopy revealed the early formation and accumulation of typical double-membrane vesicles, which probably carry the viral replication complex. The vesicles appear to be fragile, and their preservation was significantly improved by using cryofixation protocols and freeze substitution methods. In immunoelectron microscopy, the virus-induced vesicles could be labeled with replicase-specific antibodies. Opposite to what was described for mouse hepatitis virus, we did not observe the late relocalization of specific replicase subunits to the presumed site of virus assembly, which was labeled using an antiserum against the viral membrane protein. This conclusion was further supported using organelle-specific marker proteins and electron microscopy. Similar morphological studies and labeling experiments argued against the previously proposed involvement of the autophagic pathway as the source for the vesicles with which the replicase is associated and instead suggested the endoplasmic reticulum to be the most likely donor of the membranes that carry the SARS-CoV replication complex.
authors.affiliation: Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands. e.j.snijder@lumc.nl
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Snijder
authors.forename: Yvonne
authors.initials: Y
authors.lastname: van der Meer
authors.forename: Jessika
authors.initials: J
authors.lastname: Zevenhoven-Dobbe
authors.forename: Jos J M
authors.initials: JJ
authors.lastname: Onderwater
authors.forename: Jannes
authors.initials: J
authors.lastname: van der Meulen
authors.forename: Henk K
authors.initials: HK
authors.lastname: Koerten
authors.forename: A Mieke
authors.initials: AM
authors.lastname: Mommaas
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000382:microbiology / Q000821:virology; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis; D018741:Replication Origin; D045473:Severe acute respiratory syndrome-related coronavirus; D022161:Transport Vesicles / Q000648:ultrastructure*; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D012324:RNA-Dependent RNA Polymerase