title: Neonatal hypothyroidism alters the kinetic properties of Na+, K+-ATPase in synaptic plasma membranes from rat brain.
abstract: Neonatal hypothyroidism was induced in rat pups by injecting 131I within two days of birth and the effects on kinetic properties of Na+, K+-ATPase from synaptic plasma membranes were examined. Neonatal hypothyroidism resulted in a generalized decrease in V(max) with ATP, Na+, K+ and Mg2+ together with an increase in the K(m) for ATP, appearance of a low affinity component for Na+ and allosteric characteristic for the Mg2+-dependent activity at high Mg2+ concentrations. Binding pattern for Na+ and Mg2+ changed. Our results suggest that impairment of Na+, K+-ATPase activity together with altered kinetic properties could be one of the underlying biochemical mechanism leading to central nervous system (CNS) dysfunctions as a consequence of thyroid hormone deprivation during critical stages of brain development.
authors.affiliation: Department of Biochemistry, Terna Medical College, Nerul, Navi Mumbai 400706, India.
authors.forename: Framroze R
authors.initials: FR
authors.lastname: Billimoria
authors.forename: Bharat N
authors.initials: BN
authors.lastname: Dave
authors.forename: Surendra S
authors.initials: SS
authors.lastname: Katyare
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology* / Q000473:pathology; D004789:Enzyme Activation; D005260:Female; D007037:Hypothyroidism / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D007456:Iodine Isotopes / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013570:Synaptic Membranes / Q000201:enzymology*
chemical_list: D007456:Iodine Isotopes; D000254:Sodium-Potassium-Exchanging ATPase