title: [Gas gangrene--case diagnosis].
abstract: Anaerobic spore-forming bacteria from species Clostridium perfringens is commonly present in soil and in the intestines of men and animals. Clostridium perfringens type A are responsible for gas gangrene men and animals. This paper describes gas gangrene case after limb amputation at 69-year-old man, treated for diabetes since several years. Used multiplex PCR generated amplification product 900 bp size (cpa) responsible for coding alpha toxin (CPA) present at Clostridium perfringens type A.
authors.affiliation: Zakład Higieny Srodków Zywienia Zwierzat, Państwowy Instytut Weterynaryjny, Państwowy Instytut Badawczy. elawoj@piwet.pulawy.pl
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Wojdat
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kwiatek
authors.forename: Henryk
authors.initials: H
authors.lastname: Zdrojewski
authors.forename: Lidia
authors.initials: L
authors.lastname: Krupa
mesh_terms: D000368:Aged; D001427:Bacterial Toxins / Q000302:isolation & purification*; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D003016:Clostridium perfringens / Q000302:isolation & purification*; D017719:Diabetic Foot / Q000601:surgery; D005738:Gas Gangrene / Q000382:microbiology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D010738:Type C Phospholipases / Q000302:isolation & purification*
chemical_list: D001427:Bacterial Toxins; D002135:Calcium-Binding Proteins; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens