title: Isolation and characterization of a new simian rotavirus, YK-1.
abstract: BACKGROUND
  To effectively analyze the requirements for protection to rotavirus infection, a reliable animal model that reasonably mimics infection and disease in humans is needed. A requirement for an effective animal model is the availability of appropriate rotavirus stocks for challenge.
  
  
  RESULTS
  A new simian rotavirus, designated YK-1, was isolated from a 2-year-old immunodeficient pigtailed macaque with chronic diarrhea. YK-1 was distinguishable by electropherotype from the other simian rotavirus strains, SA11 and RRV. One variant of YK-1, clone 311, which was isolated after adaptation and plaque purification in cell cultures, displayed an unusual RNA electropherotype with an abnormally migrating gene 11 segment. Sequence analysis demonstrated a genetic rearrangement that involved a partial duplication of the gene 11 ORF encoding NSP5. YK-1 was identified as a Group A rotavirus belonging to subgroup 1. To further characterize the YK-1 strain, the genes encoding VP4, VP7, and NSP4 were sequenced. Analysis of VP4 and VP7 gene fragments suggests that this strain is a G3P3 rotavirus and is closely related to the simian rotavirus strain RRV. Serotype analysis also identified YK-1 as a G3 rotavirus. The NSP4 genotype of YK-1 is C, the same genotype as RRV.
  
  
  CONCLUSIONS
  This newly isolated rotavirus, YK-1, is being used to establish a nonhuman primate model for studying the infectivity, immunity, and pathogenesis of rotavirus and for evaluating candidate rotavirus vaccines.
authors.affiliation: Viral Gastroenteritis Team, Respiratory and Enteric Viruses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. larry@cidrz.org
authors.forename: Larry E
authors.initials: LE
authors.lastname: Westerman
authors.forename: Baoming
authors.initials: B
authors.lastname: Jiang
authors.forename: Harold M
authors.initials: HM
authors.lastname: McClure
authors.forename: Lauren J
authors.initials: LJ
authors.lastname: Snipes-Magaldi
authors.forename: Dixie D
authors.initials: DD
authors.lastname: Griffin
authors.forename: Gary
authors.initials: G
authors.lastname: Shin
authors.forename: Jon R
authors.initials: JR
authors.lastname: Gentsch
authors.forename: Roger I
authors.initials: RI
authors.lastname: Glass
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D005243:Feces / Q000821:virology; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D008254:Macaca nemestrina; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology*; D012367:RNA, Viral; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D012703:Serotyping; D014118:Toxins, Biological / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D012367:RNA, Viral; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins