title: Application of anodized aluminum in fluorescence detection of biological species.
abstract: Thin nanoporous alumina obtained by anodization of aluminum films offers promising advantages for application in fluorescence-based biological sensors including convenient preparation, increased density of binding sites, and improved collection efficiency of fluorescence. These advantages are illustrated in the detection of streptavidin using biotin covalently bound to the surface of alumina nanopores. Fluorescence intensity enhancement as high as 7 times is observed in nanopores in comparison to flat glass surface.
authors.affiliation: Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA.
authors.forename: Pavel
authors.initials: P
authors.lastname: Takmakov
authors.forename: Ivan
authors.initials: I
authors.lastname: Vlassiouk
authors.forename: Sergei
authors.initials: S
authors.lastname: Smirnov
mesh_terms: D000535:Aluminum / Q000032:analysis* / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry; D007477:Ions / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D019809:Streptavidin / Q000737:chemistry
chemical_list: D007477:Ions; D012996:Solutions; D001710:Biotin; D019809:Streptavidin; D000535:Aluminum