Improving access to the control region and tRNA gene clusters of dipteran mitochondrial DNA.
Despite the successful use of universal primers for amplifying insect mtDNA, specific regions remain difficult to recover and demand the use of taxon-specific primers. In this work, we describe a new set of primers for efficiently amplifying and sequencing the mtDNA control region and three tRNA gene clusters of dipterans of medical and veterinary importance, including Muscidae, Calliphoridae, and Oestridae species. These new primers were useful for investigating the nucleotide information and the structural organization of dipteran mtDNA.
Oliveira
M T
MT
Laboratório de Genética Animal, Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP 6010, CEP 13083-875, Campinas, São Paulo, Brazil. mto@unicamp.br
Da Rosa
A C
AC
Azeredo-Espin
A M L
AM
Lessinger
A C
AC
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D004175:Diptera / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012343:RNA, Transfer / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004272:DNA, Mitochondrial; D012343:RNA, Transfer