Effects of two treatments on semen from different bulls on in vitro fertilization results of bovine oocytes.
The semen of six different bulls was used to examine the effects of treatment with caffeine or caffeine plus Ca-ionophre on in vitro fertilization, cleavage and development into morulae of in vitro matured bovine oocytes. In vitro fertilization results (formation of both pronuclei, cleavage and development to >or= four-cell stage were significantly (P<0.01) higher using caffeine plus Ca-ionophre than those using only caffeine. The rates of fertilization and first cleavage were only slightly variable among the bulls. However, the present data showed significant variability in formation of both pronuclei (36 to 75%) of fertilized ova and development to the >or=4 cell stage (39 to 71%) by different bulls. Development into morulae of ova recovered from the rabbit oviduct did not show any significant differences in relation to sperm treatments or individual bulls.
Aoyagi
Y
Y
Hokkaido Livestock Improvement Association Inc., Kunneppu, Tokorogun 099-14, Japan.
Fujii
K
K
Iwazumi
Y
Y
Furudate
M
M
Fukui
Y
Y
Ono
H
H