Simultaneous electrochemical determination of uric acid and ascorbic acid on a glassy carbon electrode modified with cobalt(II) tetrakisphenylporphyrin.
A cobalt(II) tetrakisphenylporphyrin (Co(II)TPP) film modified glassy carbon electrode (Co(II)TPP-GCE) was prepared by just coating Co(II)TPP solution on the surface of the electrode. It can be used for the simultaneous determination of ascorbic acid and uric acid. The anodic peaks of AA and UA can be separated well. Owing to the strongly hydrophobic property of porphyrin, the modified electrode has good stability and long life. The linear range for UA and AA were 2.0 x 10(-6)-1.0 x 10(-4) M and 9.0 x 10(-6)-2.0 x 10(-3) M with detection limits of 5.0 x 10(-7) and 5.0 x 10(-6) M, respectively. Furthermore, metalloporphyrins of other kinds were also used to construct modified electrodes. Their performances were inferior compared with that of the Co(II)TPP modified electrode.
Li
Chun-Xiang
CX
Chemistry and Chemical Engineering College, Hunan University of Science and Technology, Xiangtan, P R China. cxli@hnust.edu.cn
Zeng
Yun-Long
YL
Liu
Ying-Ju
YJ
Tang
Chun-Rang
CR
D001205:Ascorbic Acid / Q000032:analysis* / Q000652:urine; D002244:Carbon / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008665:Metalloporphyrins / Q000737:chemistry*; D008670:Metals / Q000737:chemistry; D015203:Reproducibility of Results; D014527:Uric Acid / Q000032:analysis* / Q000652:urine
D008665:Metalloporphyrins; D008670:Metals; D014527:Uric Acid; D003035:Cobalt; D002244:Carbon; D001205:Ascorbic Acid