title: Direct immobilization of tyrosinase enzyme from natural mushrooms (Agaricus bisporus) on D-sorbitol cinnamic ester.
abstract: Mushroom tyrosinase was immobilized from an extract onto the totally cinnamoylated derivative of D-sorbitol by direct adsorption as a result of the intense hydrophobic interactions that took place. The immobilization pH value and mass of lyophilized mushrooms were important parameters that affected the immobilization efficiency, while the immobilization time and immobilization support concentration were not important in this respect. The extracted/immobilized enzyme could best be measured above pH 3.5 and the optimum measuring temperature was 55 degrees C. The apparent Michaelis constant using 4-tert-butylcatechol as substrate was 0.38+/-0.02 mM, which was lower than for the soluble enzyme from Sigma (1.41+/-0.20 mM). Immobilization stabilized the extracted enzyme against thermal inactivation and made it less susceptible to activity loss during storage. The operational stability was higher than in the case of the tyrosinase supplied by Sigma and immobilized on the same support. The results show that the use of p-nitrophenol as enzyme-inhibiting substrate during enzyme extraction and immobilization made the use of ascorbic acid unnecessary and is a suitable method for extracting and immobilizing the tyrosinase enzyme, providing good enzymatic activity and stability.
authors.affiliation: Grupo de Química de Carbohidratos y Biotecnología de Alimentos (QCBA), Departamento de Química Orgánica, Facultad de Química, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain.
authors.forename: María Elisa
authors.initials: ME
authors.lastname: Marín-Zamora
authors.forename: Francisco
authors.initials: F
authors.lastname: Rojas-Melgarejo
authors.forename: Francisco
authors.initials: F
authors.lastname: García-Cánovas
authors.forename: Pedro Antonio
authors.initials: PA
authors.lastname: García-Ruiz
mesh_terms: D000364:Agaricus / Q000201:enzymology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D011485:Protein Binding; D013012:Sorbitol / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D013012:Sorbitol; D014442:Monophenol Monooxygenase