Unusual type of TLS/FUS-ERG chimeric transcript in a pediatric acute myelocytic leukemia with 47,XX,+10,t(16;21)(p11;q22).
We report on a case of pediatric acute myelocytic leukemia showing 47,XX,+10,t(16;21)(p11;q22) that resulted in an unusual TLS/FUS-ERG chimeric transcript. The leukemic cells showed erythrophagocytosis, positive reactions for myeloperoxidase and Sudan black B stains, and negative reactions for periodic acid-Schiff and alpha-naphtyl butyrate esterase stains as well as expression of myeloid antigens. We also confirmed a very rare type of TLS/FUS-ERG chimeric transcript by fusion of the 5' part of the TLS/FUS gene in chromosome 16p11 and the 3' part of the ERG gene in chromosome 21q22 using reverse-transcriptase polymerase chain reaction and direct sequencing. After achieving a complete remission with two cycles of induction chemotherapy, the patient received an umbilical cord blood transplantation.
Choi
Hyun-Woo
HW
Department of Laboratory Medicine, Chonnam National University Hwasun Hospital, Ilsimri-160, Hwasun-eup, Hwasun-gun, South Korea 519-809.
Shin
Myung-Geun
MG
Sawyer
Jeffrey R
JR
Cho
Duck
D
Kee
Seung-Jung
SJ
Baek
Hee-Jo
HJ
Kook
Hoon
H
Kim
Hyeoung-Joon
HJ
Shin
Jong-Hee
JH
Suh
Soon-Pal
SP
Hwang
Tai-Ju
TJ
Ryang
Dong-Wook
DW
D001854:Bone Marrow Cells / Q000166:cytology; D002675:Child, Preschool; D002879:Chromosomes, Human, Pair 10; D002885:Chromosomes, Human, Pair 16 / Q000648:ultrastructure; D002891:Chromosomes, Human, Pair 21 / Q000648:ultrastructure; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D034702:RNA-Binding Protein FUS / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014178:Translocation, Genetic; D014314:Trisomy
D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; D034702:RNA-Binding Protein FUS; C107302:TLS-ERG fusion protein, human