Efficient polymer monolith for strong cation-exchange capillary liquid chromatography of peptides.
A stable poly[2-acrylamido-2-methyl-1-propanesulfonic acid-co-poly(ethylene glycol) diacrylate] monolith was synthesized inside a 75-microm-i.d. capillary by photoinitiated copolymerization with water, methanol, and ethyl ether as porogens. The resulting monolith was evaluated for strong cation-exchange capillary liquid chromatography of both synthetic and natural peptides. Although the monolith possessed relatively strong hydrophobicity due to the use of 2-acrylamido-2-methyl-1-propanesulfonic acid as one monomer, the monolith had a high dynamic binding capacity of 157 microequiv of peptide/mL, or 332 mg of cytochrome c/mL. Exceptionally high resolution resulting from extremely narrow peaks was obtained, resulting in a peak capacity of 179 when using a shallow salt elution gradient. Although a second, naturally formed gradient might contribute to the sharp peaks obtained, high efficiency was mainly due to the use of poly(ethylene glycol) diacrylate as a biocompatible cross-linker.
Gu
Binghe
B
Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602-5700, USA.
Chen
Zhaoyuan
Z
Thulin
Craig D
CD
Lee
Milton L
ML
D000595:Amino Acid Sequence; D002021:Buffers; D002412:Cations / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry
D002021:Buffers; D002412:Cations; D010455:Peptides; D011108:Polymers; D011506:Proteins