Are branched chain fatty acids the natural substrates for P450(BM3)?
Branched chain fatty acids are substrates for cytochrome P450(BM3) (CYP102) from Bacillus megaterium; oxidation of C15 and C17 iso and anteiso fatty acids by P450(BM3) leads to the formation of hydroxylated products that possess high levels of regiochemical and stereochemical purity.
Cryle
Max J
MJ
Department of Chemistry, University of Queensland, St. Lucia, Brisbane, Australia 4072.
Espinoza
Rocio D
RD
Smith
Sarah J
SJ
Matovic
Nicholas J
NJ
De Voss
James J
JJ
D001410:Bacillus megaterium / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry*; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D005227:Fatty Acids / Q000138:chemical synthesis / Q000737:chemistry*; D006900:Hydroxylation; D006899:Mixed Function Oxygenases / Q000737:chemistry*; D015394:Molecular Structure; D009251:NADPH-Ferrihemoprotein Reductase; D010084:Oxidation-Reduction; D013237:Stereoisomerism; D013379:Substrate Specificity
D001426:Bacterial Proteins; D005227:Fatty Acids; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases