title: In vitro fertilization of bovine follicular oocytes recovered by laparoscopy.
abstract: Thirty-three laparoscopies were performed on 16 follicle stimulating hormone (FSH)-superovulated cows (one to four laparoscopies per cow). Two hundred and four follicles were aspirated (6.18/cow) and 157 oocytes (77% recovery rate) were isolated. Sixty percent of the oocytes found were considered to be of good to excellent quality. In cows with ongoing ovulations at laparoscopy only 32% of the oocytes were suitable for fertilization. Twenty-four percent of the oocytes from cows with no ovulation and 16% from cows with ovulations were fertilized. Higher proportions of oocytes were fertilized in cows showing estrous behavior vs cows with no signs of estrus (25 vs 15%). Using lysolecithin-treated spermatozoa, 28% of the oocytes with good to excellent quality were fertilized, and 35% of the fertilized eggs cleaved in vitro to two- to four-cell stages.
authors.affiliation: Department of Physiology and Pharmacology College of Veterinary Medicine The University of Georgia Athens, GA 30602 USA.
authors.forename: K
authors.initials: K
authors.lastname: Schellander
authors.forename: R A
authors.initials: RA
authors.lastname: Fayrer-Hosken
authors.forename: C L
authors.initials: CL
authors.lastname: Keefer
authors.forename: L M
authors.initials: LM
authors.lastname: Brown
authors.forename: H
authors.initials: H
authors.lastname: Malter
authors.forename: C E
authors.initials: CE
authors.lastname: M C Bride
authors.forename: B G
authors.initials: BG
authors.lastname: Brackett