Applications of chemical-inducible expression systems in functional genomics and biotechnology.
The LexA-VP16-ER (XVE) system is an estrogen-receptor-based chemical-inducible system for use in plants. Protocols are outlined for the inducible overexpression of transgenes, inducible transgene excision, inducible gene silencing, regulated in vivo cDNA generation, inducible activation tagging, and the generation of conditional knockouts.
Zuo
Jianru
J
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing.
Hare
Peter D
PD
Chua
Nam-Hai
NH
D017360:Arabidopsis / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D005821:Genetic Techniques; D008957:Models, Genetic; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D034622:RNA Interference; D011960:Receptors, Estrogen / Q000235:genetics
D018076:DNA, Complementary; D010940:Plant Proteins; D011960:Receptors, Estrogen