title: Elevated mutation rates in the germline of Polkappa mutant male mice.
abstract: Mutation rates at two expanded simple tandem repeat (ESTR) loci were studied in the germline of DNA polymerase kappa (Polkappa(-/-)) deficient mice. The spontaneous mutation rate in homozygous Polkappa(-/-) males was significantly higher than in isogenic wild-type mice (Polkappa(+/+)), but the ESTR mutation spectrum in Polkappa(-/-) animals did not differ from that in Polkappa(+/+) males. We suggest that compromised translesion synthesis in Polkappa(-/-) mice may result in replication fork pausing which, in turn, may affect ESTR mutation rate.
authors.affiliation: Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
authors.forename: Karen L-A
authors.initials: KL
authors.lastname: Burr
authors.forename: Susana
authors.initials: S
authors.lastname: Velasco-Miguel
authors.forename: Venkata S
authors.initials: VS
authors.lastname: Duvvuri
authors.forename: Lisa D
authors.initials: LD
authors.lastname: McDaniel
authors.forename: Errol C
authors.initials: EC
authors.lastname: Friedberg
authors.forename: Yuri E
authors.initials: YE
authors.lastname: Dubrova
mesh_terms: D000818:Animals; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000172:deficiency / Q000235:genetics*; D018095:Germ-Line Mutation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D018598:Minisatellite Repeats; D000099270:Y-Family DNA Polymerases
chemical_list: D004259:DNA-Directed DNA Polymerase; C498613:Polk protein, mouse; D000099270:Y-Family DNA Polymerases