Simultaneous determination of geniposide, baicalin, cholic acid and hyodeoxycholic acid in rat serum for the pharmacokinetic investigations by high performance liquid chromatography-tandem mass spectrometry.
A simple, rapid, and specific analytical method for simultaneous determination of geniposide, baicalin, cholic acid and hyodeoxycholic acid in 50 microL samples of rat serum was developed by high performance liquid chromatography-tandem mass spectrometry. The quantification of the target compounds was determined by multiple reaction monitoring (MRM) mode using electrospray ionization (ESI). The correlation coefficients of the calibration curves were better than 0.997. The intra- and inter-day accuracy, precision, and linear range had been investigated in detail. This method was subsequently applied to pharmacokinetic studies of geniposide, baicalin, cholic acid and hyodeoxycholic acid in rats successfully.
Ran
Xiaorong
X
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Liang
Qionglin
Q
Luo
Guoan
G
Liu
Qingfei
Q
Pan
Yanshu
Y
Wang
Bing
B
Pang
Chunhong
C
D000818:Animals; D002138:Calibration; D019826:Cholic Acid / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003840:Deoxycholic Acid / Q000097:blood* / Q000493:pharmacokinetics; D005419:Flavonoids / Q000097:blood* / Q000493:pharmacokinetics; D039823:Iridoids / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D011714:Pyrans / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D005419:Flavonoids; D039823:Iridoids; D011714:Pyrans; D003840:Deoxycholic Acid; C007835:geniposide; C038044:baicalin; C010471:hyodeoxycholic acid; D019826:Cholic Acid