Electrical stimulation in normal and retinal degeneration (rd1) isolated mouse retina.
Stimulus threshold and response latencies were measured for electrically elicited retinal ganglion cell responses in retina isolated from the eyes of normal and retinal degenerate (rd1) mice. Stimulation of the ganglion cell-side in normal retina yielded a significantly lower mean threshold and shorter latency when compared with stimulation of the photoreceptor side in normal retina. The latency of the ganglion cell-side stimulation in normal retina also proved to be significantly shorter than the latency for stimulation of the ganglion cell side in rd1 retina. Thus both the electrode positioning as well as the health of the retinal tissue play a role in the stimulating current required to elicit a retinal response.
O'Hearn
Thomas M
TM
The Doheny Eye Institute, The Keck School of Medicine at the University of Southern California, Los Angeles, CA, USA. ohearn@usc.edu
Sadda
Srinivas R
SR
Weiland
James D
JD
Maia
Mauricio
M
Margalit
Eyal
E
Humayun
Mark S
MS
D000818:Animals; D004558:Electric Stimulation; D004566:Electrodes; D004594:Electrophysiology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011930:Reaction Time; D012162:Retinal Degeneration / Q000503:physiopathology*; D012165:Retinal Ganglion Cells / Q000502:physiology*; D012684:Sensory Thresholds