Extracellular phosphate alters cementoblast gene expression.
Genetic data from humans and mice reveal that the formation of cementum is sensitive to intra- and extracellular phosphate/pyrophosphate distribution. The intracellular molecular pathways whereby altered levels of extracellular phosphate concentration may affect cementum formation have not been elucidated. To initiate inquiry, we have studied the temporal effects of extracellular phosphate on global patterns of gene expression in a line of immortalized mouse cementoblasts. Total RNA from cultured cementoblasts treated with 5 mM inorganic phosphate over a designated time period, from 1-48 hrs, was analyzed for global patterns of gene expression by means of DNA microarrays representing the complete mouse genome. Analyses of significant hybridization signals indicated that 5 mM extracellular phosphate alters the expression of genes comprising several gene ontology (GO) groups, including transcription factor activity and Wnt signaling.
Rutherford
R B
RB
Department of Oral Biology, School of Dentistry, University of Washington, Box 357132, Seattle, WA 98195-7132, USA. rbruth@u.washington.edu
Foster
B L
BL
Bammler
T
T
Beyer
R P
RP
Sato
S
S
Somerman
M J
MJ
D000818:Animals; D002478:Cells, Cultured; D025021:Cementogenesis / Q000187:drug effects* / Q000235:genetics; D003739:Dental Cementum / Q000187:drug effects*; D005110:Extracellular Space; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010710:Phosphates / Q000494:pharmacology*; D012313:RNA / Q000032:analysis / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014157:Transcription Factors / Q000187:drug effects; D051153:Wnt Proteins / Q000187:drug effects
D010710:Phosphates; D014157:Transcription Factors; D051153:Wnt Proteins; D012313:RNA