title: Determination of Thiamethoxam residues in honeybees by high performance liquid chromatography with an electrochemical detector and post-column photochemical reactor.
abstract: A method for the determination of Thiamethoxam in bee samples was set up by means of high performance liquid chromatography with an electrochemical detector and post-column photochemical reactor (HPLC-h nu-ED). Analytical method was based on a rapid sample extraction procedure with acetone, followed by chromatographic separation into a C18-RP column isocratically operated by 60 mM phosphate buffer/acetonitrile (75/25) mobile phase at pH 2.7. A photochemical reactor was used as a tool to verify and eventually quantify the presence of Thiamethoxam in the samples by distinguishing it from interference contribution. Detection was performed with a potential of 880 mV after a photoactivation with a 254 nm light. The least detectable dose was 0.002 mg kg(-1). Recovery rates ranged between 59.88 and 71.62%.
authors.affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Istituto Nazionale di Apicoltura, Via di Saliceto 80, 40128 Bologna, Italy. mrancan@inapicoltura.org
authors.forename: Milena
authors.initials: M
authors.lastname: Rancan
authors.forename: Simona
authors.initials: S
authors.lastname: Rossi
authors.forename: Anna Gloria
authors.initials: AG
authors.lastname: Sabatini
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000032:analysis*; D010078:Oxazines / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D010777:Photochemistry / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D000077922:Thiamethoxam; D013844:Thiazoles
chemical_list: D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D010573:Pesticide Residues; D013844:Thiazoles; D000077922:Thiamethoxam