title: [Separation and purification of skeletal muscle satellite cells for tissue engineering applications by Percoll].
abstract: OBJECTIVE
  To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique.
  
  
  METHODS
  The primary skeletal muscle satellite cells of New Zealand white rabbits were cultured with different adhesion time method and incontinuous density Percoll gradient centrifugation technique. The cells were observed under invert microscope and scanning electron microscope. The degree of purification was examined by celluar immunochemical stain. The growth curve was tested by thiazolyl blue assay.
  
  
  RESULTS
  Over 90% satellite cells were harvested by incontinuous density Percoll gradient centrifugation technique, in contrast to which, only 30%-40% cells were harvested by different adhesion time. Morphological observation accorded with satellite cells. The growth curve indicated that the cells grew in a good status.
  
  
  CONCLUSIONS
  The high purification satellite cells can be obtained by incontinuous density Percoll gradient centrifugation technique. It is a good method to culture seed cells for tissue engineering applications.
authors.affiliation: Department of Urology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. zhangyushi@126.com
authors.forename: Yu-shi
authors.initials: YS
authors.lastname: Hang
authors.forename: Han-zhong
authors.initials: HZ
authors.lastname: Li
authors.forename: Rui-qiang
authors.initials: RQ
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Wei-gang
authors.initials: WG
authors.lastname: Yan
mesh_terms: D000818:Animals; D021522:Bioartificial Organs; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D008297:Male; D011205:Povidone; D011817:Rabbits; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology*; D012822:Silicon Dioxide; D023822:Tissue Engineering; D001743:Urinary Bladder
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone