Diethylamine extraction of proteins and peptides isolated with a mono-phasic solution of phenol and guanidine isothiocyanate.
Representative extraction of both RNA and protein from a single biological sample is required for reliable assessment of coordinated changes in gene and protein expression. Such a simultaneous extraction can be performed by using Trizol Reagent. Here, we demonstrate that, as an alternative to SDS, 2% diethylamine is an effective solvent, which can be conveniently used in extraction of Trizol-isolated proteins from various tissues. Diethylamine provides efficient extraction of proteins and compatibility with a variety of common downstream analytical applications.
Nolan
Rebecca L
RL
Department of Physiology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
Teller
Jan K
JK
D001921:Brain; D001923:Brain Chemistry; D004048:Diethylamines / Q000737:chemistry*; D006146:Guanidines / Q000737:chemistry*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000302:isolation & purification*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D010636:Phenols / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D004048:Diethylamines; D006146:Guanidines; D009419:Nerve Tissue Proteins; D010455:Peptides; D010636:Phenols; D011506:Proteins; C411644:trizol; C034281:diethylamine