Molecular analysis of T-cell receptor beta genes in cutaneous T-cell lymphoma reveals Jbeta1 bias.
Molecular characterization of T-cell receptor junctional region sequences in cutaneous T-cell lymphoma had not been previously reported. We have examined in detail the features of the T-cell receptor beta (TCRB) gene rearrangements in 20 individuals with well-defined stages of cutaneous T-cell lymphoma (CTCL) comprising 10 cases with early-stage mycosis fungoides (MF) and 10 cases with late-stage MF or Sezary syndrome. Using BIOMED-2 PCR primers, we detected a high frequency of clonally rearranged TCR gamma and TCRB genes (17/20 and 15/20 cases, respectively). We carried out sequencing analysis of each complete clonal variable (V)beta-diversity (D)beta-joining(J)beta fingerprint generated by PCR amplification, and determined the primary structure of the Vbeta-Dbeta-Jbeta junctional regions. We observed considerable diversity in the T-cell receptor Vbeta gene usage and complementarity-determining region 3 loops. Although we found that TCRB gene usage in CTCL and normal individuals share common features, our analysis also revealed preferential usage of Jbeta1 genes in all cases with advanced stages of disease.
Morgan
Suzanne M
SM
Molecular Pathology, Cancer Sciences Division, School of Medicine, General Hospital, Southampton University Hospitals NHS Trust, Southampton, UK.
Hodges
Elizabeth
E
Mitchell
Tracey J
TJ
Harris
Susan
S
Whittaker
Sean J
SJ
Smith
John L
JL
D015972:Gene Expression Regulation, Neoplastic; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics*; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D006801:Humans; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000276:immunology; D009182:Mycosis Fungoides / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D012751:Sezary Syndrome / Q000235:genetics / Q000276:immunology; D012878:Skin Neoplasms / Q000235:genetics* / Q000276:immunology