title: The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst.
abstract: Schistosoma mansoni eggs, miracidia and primary sporocysts were labelled with phalloidin-rhodamine to visualize filamentous actin structures. Analysis of these forms by confocal fluorescence microscopy revealed the presence of previously well-defined circular and longitudinal muscle layers. Besides these muscular layers that sustain and provide motility to these parasite forms, we found in these 3 consecutive developmental stages of the parasite previously unidentified actin-rich tubular structures. In the 3 forms, 4 actin-rich tubules could be observed by optical sectioning underneath the well-developed muscle layers. The tubules appear in pairs, transversal to the length of the parasite, and located towards the extremities. By using an anti-flame cell specific antibody we confirmed that the tubules co-localize with flame cells and also determined that the tubule core is filled with microtubules. The additional presence of myosin in these tubules strongly suggests that they are contractile structures.
authors.affiliation: Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou-FIOCRUZ, Av. Augusto de Lima 1715, 30190-002, Belo Horizonte, MG, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Bahia
authors.forename: L G A
authors.initials: LG
authors.lastname: Avelar
authors.forename: F
authors.initials: F
authors.lastname: Vigorosi
authors.forename: D
authors.initials: D
authors.lastname: Cioli
authors.forename: G C
authors.initials: GC
authors.lastname: Oliveira
authors.forename: R A
authors.initials: RA
authors.lastname: Mortara
mesh_terms: D008841:Actin Cytoskeleton / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D000199:Actins / Q000032:analysis / Q000276:immunology; D000818:Animals; D000909:Antibodies, Helminth / Q000378:metabolism; D008018:Life Cycle Stages / Q000502:physiology*; D018613:Microscopy, Confocal / Q000379:methods; D020409:Molecular Motor Proteins / Q000032:analysis* / Q000276:immunology; D009132:Muscles / Q000737:chemistry / Q000648:ultrastructure; D009218:Myosins / Q000276:immunology / Q000378:metabolism; D033661:Oocysts / Q000648:ultrastructure; D012550:Schistosoma mansoni / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure*
chemical_list: D000199:Actins; D000909:Antibodies, Helminth; D020409:Molecular Motor Proteins; D009218:Myosins