title: Purification and characterisation of a protease inhibitor from Streptomyces chromofuscus 34-1 with an antiviral activity.
abstract: The aim of this study was to purify and characterise a novel protease inhibitor (PISC-2002) isolated from culture supernatants of Streptomyces chromofuscus. PISC-2002 was purified by anion-exchange chromatography, and RP-HPLC analysis. PISC-2002 had a molecular mass of 11.2 kDa and a high content of hydrophobic amino acids and proline. N-terminal sequence gave two sequences differing by one residue. The main sequence is ASLPAVSALVLTV and the shorter sequence is SLPAVSALVLTV. This shows its homology to Streptomyces subtilisin inhibitor family. Besides its large spectrum of powerful inhibitory activities against various serine proteases, PISC-2002 displayed significant antiviral effect against influenza virus A/Rostock/34 (H7N7).
authors.affiliation: Department of Microbiology, Sofia University, Sofia, Bulgaria.
authors.forename: Lidiya
authors.initials: L
authors.lastname: Angelova
authors.forename: Michèle
authors.initials: M
authors.lastname: Dalgalarrondo
authors.forename: Ignat
authors.initials: I
authors.lastname: Minkov
authors.forename: Svetla
authors.initials: S
authors.lastname: Danova
authors.forename: Nicolai
authors.initials: N
authors.lastname: Kirilov
authors.forename: Julia
authors.initials: J
authors.lastname: Serkedjieva
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chobert
authors.forename: Thomas
authors.initials: T
authors.lastname: Haertlé
authors.forename: Iskra
authors.initials: I
authors.lastname: Ivanova
mesh_terms: D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D053126:Influenza A Virus, H7N7 Subtype / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011480:Protease Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology*; D013302:Streptomyces / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D011480:Protease Inhibitors