title: Immunologic effects of mistletoe lectins: a placebo-controlled study in healthy subjects.
abstract: The subcutaneous application of lectin-rich mistletoe preparations such as Iscador Quercus (IQ; Weleda Company, Schwäbisch Gmünd, Germany) results in a peripheral eosinophilia. Our goal was to investigate whether this effect is related to mistletoe lectin (ML) and whether it is caused by a response of the specific immune system. In a double-blinded study, 43 volunteers were randomized to one of four treatment groups: (1) IQ, (2) ML that was derived from IQ, (3) IQ that was depleted of ML, and (4) placebo. The respective preparations were applied subcutaneously twice per week for 8 weeks, in increasing doses. Weekly the differential blood count was analyzed. Every 4 weeks interferon-gamma, interleukin-5 (IL-5), and granulocyte-macrophage colony stimulating factor (GM-CSF) were determined in cultures from peripheral mononuclear cells after stimulation with IQ. IQ and ML resulted in significant eosinophilia compared with placebo and ML-depleted IQ. Furthermore, the leukocyte and granulocyte counts were increased in the IQ and ML groups compared with placebo. GM-CSF, interferon-gamma, and IL-5 increased after ex vivo in vitro stimulation with IQ in the IQ and ML groups, and were significantly different from placebo in the IQ group but not in the ML group. Eosinophilia during therapy with mistletoe preparations is due to its content of ML. This effect might be related to a stimulation of IL-5 and/or GM-CSF, which was demonstrated ex vivo in vitro. ML resulted in a temporary increase of the granulocyte count, which is probably related to an acute-phase reaction.
authors.affiliation: Center for Complementary Medicine, University Hospital Freiburg, Freiburg, Germany. rhuber@uniklinik-freiburg.de
authors.forename: Roman
authors.initials: R
authors.lastname: Huber
authors.forename: Matthias
authors.initials: M
authors.lastname: Rostock
authors.forename: Roland
authors.initials: R
authors.lastname: Goedl
authors.forename: Rainer
authors.initials: R
authors.lastname: Lüdtke
authors.forename: Konrad
authors.initials: K
authors.lastname: Urech
authors.forename: Reinhild
authors.initials: R
authors.lastname: Klein
mesh_terms: D000328:Adult; D004311:Double-Blind Method; D004802:Eosinophilia / Q000139:chemically induced / Q000276:immunology; D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D028321:Plant Preparations / Q000494:pharmacology*; D010940:Plant Proteins / Q000494:pharmacology*; D011446:Prospective Studies; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000494:pharmacology*
chemical_list: D015848:Interleukin-5; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C488862:mistletoe lectin I; C488863:mistletoe lectin II; C488864:mistletoe lectin III; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D054788:Ribosome Inactivating Proteins