title: Ozone stress and antioxidant substances in Trifolium repens and Centaurea jacea leaves.
abstract: Ozone-sensitive (NC-S clone) and resistant plants (NC-R clone) of Trifolium repens and Centaurea jacea were exposed to moderate ozone concentrations in ambient air. The aim of this study was the investigation of the relation between ozone-sensitivity and leaf concentrations of antioxidants (ascorbic acid, total phenolics and total antioxidant capacity). NC-R clone showed the highest concentrations of antioxidants with 50-70% more ascorbic acid than NC-S. NC-R had about 5 times more ascorbic acid in the young leaves and 9 times more in the old leaves than Centaurea. In a fumigation experiment with acute ozone stress (100 nl L(-1)) the antioxidant levels changed profoundly. The ozone-injured leaves of NC-S had 6-8 times more total phenolics than uninjured leaves. Generally older leaves had lower antioxidant concentrations and were more prone to ozone injury than younger leaves. Ascorbic acid concentrations were closer related to the appearance of visible ozone injury than the other antioxidative parameters.
authors.affiliation: Department of Environmental Research/UU, ARC Seibersdorf Research GmbH, A-2444 Seibersdorf, Austria. y.ferreira-severino@umweltforschung.at
authors.forename: Joyce Ferreira
authors.initials: JF
authors.lastname: Severino
authors.forename: Karl
authors.initials: K
authors.lastname: Stich
authors.forename: Gerhard
authors.initials: G
authors.lastname: Soja
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis; D031179:Centaurea / Q000187:drug effects* / Q000378:metabolism; D004781:Environmental Exposure / Q000009:adverse effects; D005651:Fumigation; D010083:Oxidants, Photochemical / Q000633:toxicity*; D010126:Ozone / Q000633:toxicity*; D010636:Phenols / Q000032:analysis; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D029921:Trifolium / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010083:Oxidants, Photochemical; D010636:Phenols; D010126:Ozone; D001205:Ascorbic Acid