An efficient RNA aptamer against human influenza B virus hemagglutinin.
Aptamers are known for their higher discriminating ability between closely related molecules and their requirement for only a small region for binding, as compared to an antibody. In the present studies, we have isolated a specific RNA aptamer against the influenza virus B/Johannesburg/05/1999 by an in vitro selection procedure. The aptamer bound efficiently to the HA of influenza B and required 5 mM MgCl(2) ion for its recognition. The aptamer not only distinguished HA derived from the influenza A virus, but also inhibited HA-mediated membrane fusion.
Gopinath
Subash C B
SC
Functional Nucleic Acids Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1 Higashi, Tsukuba, Ibaraki 305-8566.
Sakamaki
Yuriko
Y
Kawasaki
Kazunori
K
Kumar
Penmetcha K R
PK
D052157:Aptamers, Nucleotide / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004910:Erythrocyte Membrane / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D009980:Influenza A virus / Q000737:chemistry / Q000378:metabolism; D009981:Influenza B virus / Q000737:chemistry / Q000378:metabolism*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D008561:Membrane Fusion / Q000187:drug effects; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011485:Protein Binding / Q000187:drug effects; D052156:SELEX Aptamer Technique / Q000379:methods; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D013045:Species Specificity
D052157:Aptamers, Nucleotide; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D008274:Magnesium