title: Microwave assisted "click" chemistry for the synthesis of multiple labeled-carbohydrate oligonucleotides on solid support.
abstract: A versatile approach has been developed for the multiple labeling of oligonucleotides. First, three linkers as a H-phosphonate monoester derivative were condensed on a solid-supported T12 to introduce H-phosphonate diester linkages which were oxidized in the presence of propargylamine. Second, three galactosyl azide derivatives were conjugated to the solid-supported three-alkyne-modified T12 by a 1,3-cycloaddition so-called "click chemistry" in the presence of Cu(I) assisted by microwaves.
authors.affiliation: Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-Université Montpellier II, Place E. Bataillon, 34095 Montpellier Cedex 5, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Bouillon
authors.forename: Albert
authors.initials: A
authors.lastname: Meyer
authors.forename: Sébastien
authors.initials: S
authors.lastname: Vidal
authors.forename: Anne
authors.initials: A
authors.lastname: Jochum
authors.forename: Yann
authors.initials: Y
authors.lastname: Chevolot
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Cloarec
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Praly
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Vasseur
authors.forename: François
authors.initials: F
authors.lastname: Morvan
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques; D003300:Copper; D008872:Microwaves; D015335:Molecular Probes / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis*
chemical_list: D002241:Carbohydrates; D015335:Molecular Probes; D009841:Oligonucleotides; D003300:Copper