[A study on the karyotype, C-banding and Ag-NORs in Rana nigromaculata].
The chromosome samples of Rana nigromaculata were prepared by peripheral blood lymphocyte cultured in vitro. Karyotype, C-banding and silver-staining nucleolus organizer regions (Ag-NORs) were observed on chromosomes samples. The result showed that: (1) the chromosome number of Rana nigromaculata is 26 (2n=26), i.e., 10 large and 16 small chromosomes, the large chromosomes are matched into 5 homologous pairs and small chromosomes are matched into 8 homologous pairs, and further indicates the karyotype of Rana nigromaculata is bi-modal; (2) Metaphases of female and male were observed separately, the chromosome of No.11 has a pronounced secondary constriction in the middle of long arms. But the position of secondary constriction in the aberrant type individuals is in the middle of long arms of the No.8; (3) it has only one obvious C-banding close to the telomere, which is located in the long arms of the No.5; (4) The chromosome No.11 is a pair of homologous chromosomes with Ag-NORs, and the position of Ag-NORs in female and male is identical.
Shi
Heng-Liang
HL
School of Life Science, Xuzhou Normal University, Xuzhou 221116, China.
Zhang
Chun
C
Wu
Mao
M
Li
Pei-Qing
PQ
Dong
Yu-Wei
YW
Pang
Yong-Hong
YH
Liu
Huan-Min
HM
Fang
Xing-Tang
XT
Gao
Jian Guo
JG
Zhu
Bi-Cai
BC
D000818:Animals; D002871:Chromosome Banding; D005260:Female; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region / Q000235:genetics*; D011898:Ranidae / Q000235:genetics*; D016622:Silver Staining