Luciferases as reporter genes.
Luciferase is the ideal reporter gene to provide temporal and spatial information on promoter activity in Arabidopsis and other eukaryotes; the noninvasive detection of luminescence and short half-life of luciferase activity allow repeated measurements of individual seedlings over several days to assay dynamic changes in gene expression. Transgenic or transiently transformed plants with a luciferase gene under the control of a promoter of interest are required. Detection of the low level of luminescence produced by the luciferase gene in Arabidopsis requires the use of low light detecting charge-coupled device (CCD) cameras or scintillation counters. This chapter contains protocols on assaying and imaging luciferase in vivo and the automation for high-resolution timecourses.
Southern
Megan M
MM
Department of Biological Sciences, University of Warwick, UK.
Brown
Paul E
PE
Hall
Anthony
A
D017360:Arabidopsis / Q000235:genetics*; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D049449:Luminescence; D008163:Luminescent Measurements; D018715:Microscopy, Video; D013997:Time Factors; D014158:Transcription, Genetic
D008156:Luciferases