title: HIV-1-induced pulmonary oxidative and nitrosative stress: exacerbated response to endotoxin administration in HIV-1 transgenic mouse model.
abstract: Human immunodeficiency virus (HIV)-1 causes lung disease by increasing the host's susceptibility to pathogens. HIV-1 also causes an increase in systemic oxidative/nitrosative stress, perhaps enhancing the deleterious effects of secondary infections. Here we examined the ability of HIV-1 proteins to increase lung oxidative/nitrosative stress after lipopolysaccharide (LPS) (endotoxin) administration in an HIV-1 transgenic mouse model. Lung oxidative/nitrosative stress biomarkers studied 3 and 6 h after LPS administration were as follows: lung edema, tissue superoxide, NO metabolites, nitrotyrosine, hydrogen peroxide, and bronchoalveolar lavage fluid (BALF) glutathione (GSH). Blood serum cytokine levels were quantified to verify immune function of our nonimmunocompromised animal model. Results indicate that 3 h after LPS administration, HIV-1 transgenic mouse lung tissue has significantly greater edema and superoxide. Furthermore, NO metabolites are significantly elevated in HIV-1 transgenic mouse BALF, lung tissue, and blood plasma compared with those of wild-type mice. HIV-1 transgenic mice also produce significantly greater lung nitrotyrosine and hydrogen peroxide than wild-type mice. In addition, HIV-1 transgenic mice produce significantly less BALF GSH than wild-type mice 3 h after LPS treatment. Without treatment, serum cytokine levels are similar for HIV-1 transgenic and wild-type mice. After treatment, serum cytokine levels are significantly elevated in both HIV-1 transgenic and wild-type mice. Therefore, HIV-1 transgenic mice have significantly greater lung oxidative/nitrosative stress after endotoxin administration than wild-type mice, independent of immune function. These results indicate that HIV-1 proteins may increase pulmonary complications subsequent to a secondary infection by altering the lung redox potential.
authors.affiliation: Department of Pulmonary and Critical Care Medicine, Center for Clinical and Molecular Nutrition, Emory University School of Medicine, Veterans Affairs Medical Center, 1670 Clairmont Rd., Atlanta, GA 30033, USA.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Jacob
authors.forename: Kristi M
authors.initials: KM
authors.lastname: Porter
authors.forename: Shawn C
authors.initials: SC
authors.lastname: Elms
authors.forename: Po-Yung
authors.initials: PY
authors.lastname: Cheng
authors.forename: Dean P
authors.initials: DP
authors.lastname: Jones
authors.forename: Roy L
authors.initials: RL
authors.lastname: Sutliff
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D015658:HIV Infections / Q000378:metabolism*; D015497:HIV-1; D006861:Hydrogen Peroxide / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D015538:Nitrosation; D018384:Oxidative Stress / Q000187:drug effects*; D011654:Pulmonary Edema / Q000139:chemically induced; D014443:Tyrosine / Q000031:analogs & derivatives / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D009566:Nitrates; D009573:Nitrites; C002744:3-nitrotyrosine; D014443:Tyrosine; D006861:Hydrogen Peroxide; D005978:Glutathione