[Diagnostic performance of specific IgM detection and genomic amplification in rubella].
Nowadays, most exanthematic diseases for which a vaccine is available affect young adults. A large percentage of these cases prove to be rubella. The aim of this study is to assess the performance of specific IgM and RT-PCR for the diagnosis of rubella infection. Fifty-nine patients with clinically suspected measles or rubella, and with available serum, whole blood, urine and pharyngeal exudate specimens were studied. RT-PCR in pharyngeal exudate was found to be the most effective marker at the start of the disease (mean, 2.5 days). IgM detection yielded a larger percentage of positive results (76.2%), but at a later time (3.7 days).
Mosquera
María del Mar
Mdel M
Servicio de Microbiología Diagnóstica, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. de Majadahonda-Pozuelo s/n, 28220 Majadahonda, Madrid, Spain. mmosquera@isciii.es
Sanz
Juan Carlos
JC
Echevarría
Juan Emilio
JE
Herranz
Nieves
N
Fernández
Marisa
M
de Ory
Fernando
F
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010614:Pharynx / Q000821:virology; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012409:Rubella / Q000097:blood / Q000175:diagnosis* / Q000652:urine / Q000821:virology; D012412:Rubella virus / Q000276:immunology
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D012367:RNA, Viral