Primary structure of cat preproendothelin-2 and cat renal mRNA expression of preproendothelin-1 and preproendothelin-2 in naturally occurring renal failure.
Endothelin (ET)-1 is involved in the pathophysiology of various renal disorders, promoting renal cellular proliferation and extracellular matrix protein accumulation, and, thus, diminishing fundamental renal function, including filtration. To determine whether ET-1 and ET-2 play a role in feline chronic renal failure, we analyzed the messenger RNA (mRNA) expression of the prepro-ET (PPET )-1 and PPET-2 genes in affected cat kidney after molecular cloning of full-length PPET-2 complementary DNA (cDNA). Conceptual analysis of the primary structure of cat PPET-2 based on the cloned sequence demonstrated that the putative regions corresponding to a mature peptide and peptidase processing sites are present in cat PPET-2. Homology analysis showed that the similarity of the cat PPET-2 amino acid sequence with those from human, mouse, rat, rabbit, dog, ferret, cow, and horse was 73.0%, 68.6%, 69.1%, 76.4%, 81.2%, 83.1%, 76.3%, and 79.2%, respectively. Analysis of PPET-1 and PPET-2 mRNA in cat by reverse transcription polymerase chain reaction showed upregulated expression of both genes in kidneys affected by renal failure.
Uchide
Tsuyoshi
T
Laboratory of Toxicology, Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, 35-1 Higashi 23-bancho, Towada, Aomori 034-8628, Japan. uchide@vmas.kitasato-u.ac.jp
Saida
Kaname
K
D000595:Amino Acid Sequence; D000818:Animals; D002371:Cat Diseases / Q000378:metabolism* / Q000503:physiopathology; D002415:Cats; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D019332:Endothelin-1 / Q000235:genetics / Q000378:metabolism*; D016232:Endothelins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000662:veterinary*; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D019332:Endothelin-1; D016232:Endothelins; D011498:Protein Precursors; D012333:RNA, Messenger; C099648:preproendothelin 2