Preparation and quality assessment of RNA from cell-specific samples obtained by laser microdissection.
Laser microdissection is a powerful tool to obtain cell-specific isolates from complex tissue samples. This chapter outlines how to prepare plant material for microdissection and methods to extract and measure high-quality RNA suitable for a variety of different downstream applications.
Kleber
Regine
R
Max-Planck-Institute of Molecular Plant Physiology, Golm, Germany.
Kehr
Julia
J
D017360:Arabidopsis / Q000235:genetics; D017343:Genes, Plant; D005821:Genetic Techniques; D007834:Lasers; D042282:Microdissection / Q000295:instrumentation* / Q000379:methods*; D018749:RNA, Plant / Q000032:analysis* / Q000302:isolation & purification
D018749:RNA, Plant