The human muscle proteome in aging.
The aim of the present study was to assess age-dependent changes of proteins in the vastus lateralis muscle of physically active elderly and young subjects by a combination of two-dimensional difference gel electrophoresis, SDS-PAGE and ESI-MS/MS. The differences observed in the elderly group included down-regulation of regulatory myosin light chains, particularly the phosphorylated isoforms, a higher proportion of myosin heavy chain isoforms 1 and 2A, and enhanced oxidative and reduced glycolytic capacity.
Gelfi
Cecilia
C
Institute of Molecular Bioimaging and Physiology, CNR, Segrate (MI), Italy. cecilia.gelfi@ibfm.cnr.it
Vigano
Agnese
A
Ripamonti
Marilena
M
Pontoglio
Alessandro
A
Begum
Shajna
S
Pellegrino
Maria Antonietta
MA
Grassi
Bruno
B
Bottinelli
Roberto
R
Wait
Robin
R
Cerretelli
Paolo
P
D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008969:Molecular Sequence Data; D009043:Motor Activity; D018482:Muscle, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism; D010766:Phosphorylation; D020543:Proteome / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
D018994:Myosin Light Chains; D020543:Proteome; D018995:Myosin Heavy Chains