Forward genetic screening of insertional mutants.
Insertional mutagenesis has contributed to the success of forward genetics in Arabidopsis thaliana. The availability of large collections of lines mutagenized by either transposon or T-DNA insertions, in combination with the systematic sequencing of insertion/genome junctions, enables the identification of mutations for any given gene in the nuclear genome of this plant species. Protocols for the identification and confirmation of mutations by forward genetics, the isolation of insertion/genome junctions, and the stabilization of transposon-induced mutations are provided, together with an overview of available insertional mutant collections and of their characteristics.
Schneider
Anja
A
Botanisches Institut der Universität zu Köln, Germany.
Leister
Dario
D
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D004251:DNA Transposable Elements; D018744:DNA, Plant; D017343:Genes, Plant; D005821:Genetic Techniques; D008957:Models, Genetic; D016254:Mutagenesis, Insertional; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction
D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; D018744:DNA, Plant