title: Propofol, an anesthetic possessing neuroprotective action against oxidative stress, promotes the process of cell death induced by H2O2 in rat thymocytes.
abstract: Propofol (2,6-diisopropylphenol) is a general anesthetic possessing a neuroprotective action against oxidative stress produced by H2O2. H2O2 induces an exposure of phosphatidylserine on outer surface of cell membranes, resulting in change in membrane phospholipid arrangement, in rat thymocytes. Since propofol is highly lipophilic, the agent is presumed to interact with membrane lipids and hence to modify the cell vulnerability to H2O2. Therefore, to test the possibility, we have examined the effect of propofol on rat thymocytes simultaneously incubated with H2O2. Although propofol (up to 30 microM) alone did not significantly affect the cell viability, the agent at 10 microM started to increase the population of dead cells in the presence of 3 mM H2O2 and the significant increase was observed at 30 microM. Propofol at clinically relevant concentrations (10-30 microM) facilitated the process of cell death induced by H2O2 in rat thymocytes. However, propofol protected rat brain neurons against the oxidative stress induced by H2O2 under same experimental condition. Therefore, the action of propofol may be dependent on the type of cells.
authors.affiliation: Department of Pharmaceutical Care and Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8512, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Chikutei
authors.forename: Tomohiro M
authors.initials: TM
authors.lastname: Oyama
authors.forename: Shiro
authors.initials: S
authors.lastname: Ishida
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Okano
authors.forename: Masako
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Matsui
authors.forename: Kanna
authors.initials: K
authors.lastname: Horimoto
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Shin-ya
authors.initials: SY
authors.lastname: Ueno
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Oyama
mesh_terms: D018686:Anesthetics, Intravenous / Q000494:pharmacology; D000818:Animals; D017304:Annexin A5 / Q000737:chemistry / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011419:Propidium / Q000737:chemistry / Q000378:metabolism; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D018686:Anesthetics, Intravenous; D017304:Annexin A5; D018696:Neuroprotective Agents; D011419:Propidium; D006861:Hydrogen Peroxide; D016650:Fluorescein-5-isothiocyanate; D015742:Propofol