title: Anti-inflammatory activity of two diterpenes of Hyptis suaveolens from El Salvador.
abstract: Separation and isolation of the two main compounds suaveolol and methyl suaveolate from leaves of chichinguaste (Hyptis suaveolens Poit., Lamiaceae) could be achieved by means of repeated column chromatography and repeated preparative thin layer chromatography. Their chemical structures were approved by MS, 1H NMR, 13C NMR and 2D-NMR experiments. The anti-inflammatory activity of the two compounds was tested for the first time as inhibition of croton oil-induced dermatitis of the mouse ear. Suaveolol and methyl suaveolate showed nearly the same dose-dependent topical anti-inflammatory activity, only two to three times lower than that of the reference drug indomethacin. The anti-inflammatory properties of these compounds could contribute to the antiphlogistic activity of extracts of Hyptis species and confirm the rational use of Hyptis suaveolens extracts in dermatological diseases.
authors.affiliation: Institute for Applied Botany, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Paolo
authors.initials: P
authors.lastname: Grassi
authors.forename: Tomás S
authors.initials: TS
authors.lastname: Urías Reyes
authors.forename: Silvio
authors.initials: S
authors.lastname: Sosa
authors.forename: Aurelia
authors.initials: A
authors.lastname: Tubaro
authors.forename: Otmar
authors.initials: O
authors.lastname: Hofer
authors.forename: Karin
authors.initials: K
authors.lastname: Zitterl-Eglseer
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer; D003872:Dermatitis / Q000188:drug therapy; D004224:Diterpenes / Q000302:isolation & purification*; D004544:El Salvador; D031331:Hyptis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008517:Phytotherapy
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004224:Diterpenes