Assembly of pseudorabies virus genome-based transfer vehicle carrying major antigen sites of S gene of transmissible gastroenteritis virus: potential perspective for developing live vector vaccines.
Two severe porcine infectious diseases, pseudorabies (PR) and transmissible gastroenteritis (TGE) caused by pseudorabies virus (PRV) and transmissible gastroenteritis virus (TGEV) respectively often result in serious economic loss in animal husbandry worldwide. Vaccination is the important prevention means against both infections. To achieve a PRV genome-based virus live vector, aiming at further TGEV/PRV bivalent vaccine development, a recombinant plasmid pUG was constructed via inserting partial PK and full-length gG genes of PRV strain Bartha K-61 amplified into pUC119 vector. In parallel, another recombinant pHS was generated by introducing a fragment designated S1 encoding the major antigen sites of S gene from TGEV strain TH-98 into a prokaryotic expression vector pP(RO)EX HTc. The SV40 polyA sequence was then inserted into the downstream of S1 fragment of pHS. The continuous region containing S1fragment, SV40 polyA and four single restriction enzyme sites digested from pHS was subcloned into the downstream of gG promoter of pUG. In addition, a LacZ reporter gene was introduced into the universal transfer vector named pUGS-LacZ. Subsequently, a PRV genome-based virus live vector was generated via homologous recombination. The functionally effective vector was purified and partially characterized. Moreover, the potential advantages of this system are discussed.
Yin
Jiechao
J
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030 Harbin, PR China.
Ren
Xiaofeng
X
Tian
Zhijun
Z
Li
Yijing
Y
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D002522:Chlorocebus aethiops; D005761:Gastroenteritis, Transmissible, of Swine / Q000276:immunology / Q000821:virology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016679:Genome, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000235:genetics*; D014709:Vero Cells; D014765:Viral Vaccines
D000956:Antigens, Viral; D014765:Viral Vaccines