title: A study on the inactivation of micro-organisms and enzymes by high pressure CO2.
abstract: This study addresses some microbial inactivation phenomena induced by high pressure CO2 over micro-organisms and enzymes. The activity of four selected enzymes was measured before and after treatment with CO2 under pressure in both buffer solutions and natural cellular environment (E. coli cells and tomato paste). Results are reported for acid phosphatase, alkaline phosphatase, ATPase, and pectinase at different conditions of temperature, CO2 pressure, and treatment time (32-40 degrees C, 85-150 bar, 30-70 min). The results obtained show that the high pressure CO2 treatment induces an inactivation of cellular enzymatic activity higher than the one caused on the same enzymes in solution. However, the measured activity difference is not caused by a damage at the enzymes molecular level but is a consequence of the permeabilization of the cellular envelopes which leads to a release of unmodified enzymes from the cells with simultaneous drop of enzymatic cellular activity. The reported data suggest that the bacterial cell death is probably due not to a selective effect of high pressure CO2 treatment but to simultaneous detrimental action of CO2 on cellular membrane and cell wall.
authors.affiliation: Histology, Microbiology and Medical Biotechnologies Department, University of Padova, Via Gabelli 63, 35121 Padova, Italy. giulio.bertoloni@unipd.it
authors.forename: Giulio
authors.initials: G
authors.lastname: Bertoloni
authors.forename: Alberto
authors.initials: A
authors.lastname: Bertucco
authors.forename: Veronica
authors.initials: V
authors.lastname: De Cian
authors.forename: Tiziana
authors.initials: T
authors.lastname: Parton
mesh_terms: D002245:Carbon Dioxide / Q000008:administration & dosage* / Q000737:chemistry*; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D006867:Hydrolases / Q000737:chemistry* / Q000187:drug effects; D050296:Microbial Viability / Q000187:drug effects; D011312:Pressure; D013242:Sterilization / Q000379:methods*
chemical_list: D002245:Carbon Dioxide; D006867:Hydrolases