title: Effect of dexamethasone on secretion of luteinizing hormone and testosterone in the boar.
abstract: Eight adult, Yorkshire-Landrace crossbred boars were used to evaluate the effects of the synthetic glucocorticoid, dexamethasone (DXM) on the secretion of luteinizing hormone (LH) and testosterone. Four treatments of 4 d each were administered: 1) 2 ml i.m. of 0.9% (w/v) NaCl solution (control); 2) DXM (2 ml i.m. as a dose of 50 mug/kg body weight, every 12 h); 3) DXM plus gonadotropin releasing hormone (GnRH; 50 mug in 1 ml i.m. every 6 h); 4) 2 ml NaCl solution i.m. plus a single dose of 50 mug i.v. GnRH. Blood samples were collected twice daily from an indwelling jugular vein catheter for 3 d and at 15 min intervals for 12 h on the fourth day. DXM treatment resulted in lower (P M0.01) testosterone values in samples collected twice daily. More frequent sampling on Day 4 revealed that DXM reduced (P<0.01) the number of pulsatile increases of LH in plasma, although the individual mean pulse areas did not fiffer between the NaCl- and DXM-treated groups. This was associated with a decreased pulse frequency of testosterone (P<0.05). GnRH plus DXM treatment caused a significant elevation (P<0.05) in mean values as well as in the mean pulse area and in the total of the individual pulse areas of LH. Pulse area and mean concentrations of testosterone were also increased (P<0.01) when GnRH was given concurrently with DXM. Comparison of a single injection of GnRH when NaCl was being administered (Treatment 4) to one of the injections of GnRH (Day 4, 0800 h, Treatment 3) revealed a subsequently greater (P<0.01) pulse area in LH above base-line during DXM treatment (7.67 +/- 1.17 ng/ml) than during the NaCl (4.17 +/- 0.73 ng/ml) treatment period. This was reflected in a greater (P<0.01) pulse increase of testosterone following the LH pulse in boars treated with DXM. It is concluded that DXM treatment in the boar can reduce the pulse frequency of LH secretion, presumably by affecting GnRH secretion, but it has less effect directly on pituitary LH synthesis and release.
authors.affiliation: Department of Biomedical Sciences University of Guelph Guelph, Ontario, Canada N1G 2W1.
authors.forename: D
authors.initials: D
authors.lastname: Gaon
authors.forename: R M
authors.initials: RM
authors.lastname: Liptrap