title: Screening of antibiotic residues in ewes' milk destined to cheese by a commercial microbiological inhibition assay.
abstract: Bulk ewes' milk from Spanish dairy farms in the Castilla-La Mancha region and destined for production of protected denomination of origin (PDO) Manchego cheese were analysed each month for antimicrobial residues during the course of 1 year. A microbiological assay specific for ewes' milk (Eclipse '100ov') was used. The number of positive samples by the Eclipse '100ov' test was 2.6%. A second examination of positives following heat treatment at 82 degrees C for 10 min showed a reduction of up to 0.9% (i.e. 63% of the samples detected corresponded to 'false-positives'). Of the confirmed positives, 25% were identified as beta-lactams by a penicillinase solution; the remainder corresponded to antimicrobial compounds that could not be identified. The month of collection was related to the probability of positive results, but to no other factors such as somatic cell counts, bacteriology or composition (fat and protein). The highest rates of positives were observed in September and October. Finally, the results were compared with those obtained by the Delvotest SP method, which showed the detection of a higher number of positive samples compared with the Eclipse '100ov' method.
authors.affiliation: Departamento de Ciencia y Tecnología Agroforestal, ETSIA, Universidad Nacional del Litoral, 3080 Esperanza, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Yamaki
authors.forename: M I
authors.initials: MI
authors.lastname: Berruga
authors.forename: R L
authors.initials: RL
authors.lastname: Althaus
authors.forename: M P
authors.initials: MP
authors.lastname: Molina
authors.forename: A
authors.initials: A
authors.lastname: Molina
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002611:Cheese / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D005260:Female; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D008826:Microbial Sensitivity Tests / Q000379:methods; D008892:Milk / Q000737:chemistry*; D012621:Seasons; D012756:Sheep
chemical_list: D000900:Anti-Bacterial Agents