title: Insemination results with slow-cooled stallion semen stored for 70 or 80 hours.
abstract: An insemination trial was conducted to evaluate the fertility of extended slow-cooled stallion spermatozoa stored for 70 h or 80 h at 5 to 7 degrees C before insemination. Then, 1 or 2 of the first sperm-rich fractions were collected with an open-ended vagina from 4 stallions. Semen from each stallion was diluted within 2 to 3 min after collection with a modified Kenney skim milk extender (6). The proportion of raw semen in the insemination doses was 24+/-6%. One insemination dose (25 to 50 ml) consisted of approximately 2 billion total spermatozoa. In the trial, palpation per rectum and ultrasonography of 34 mares (40 cycles) were performed every 12 h. The pregnancy rate per cycle (30-d) with semen stored for 70 h before insemination was 77% (17 cycles) and, with semen stored for 80 h, 57% (23 cycles). The difference was not statistically significant. The combined pregnancy rate per cycle was 65%. These results indicate that stallion semen can retain its fertilizing capacity for up to 80 h when collected and diluted using this procedure and when the inseminations are done less than 12 h after ovulation.
authors.affiliation: Department of Biochemistry and Biotechnology, University of Kuopio, Kuopio, Finland.
authors.forename: M L
authors.initials: ML
authors.lastname: Heiskanen
authors.forename: M
authors.initials: M
authors.lastname: Huhtinen
authors.forename: A
authors.initials: A
authors.lastname: Pirhonen
authors.forename: P H
authors.initials: PH
authors.lastname: Mäenpää