[Expression and catalysis of glucokinase of Thermoanaerobacter tengcongensis at different temperatures].
According to analysis of proteomic profiling for Thermoanaerobacter tencongensis, TTE0090 could be a novel gene of glucokianse (GLK), though no GLK gene was annotated in the genomic data. With the methods of cloning and expression in vitro, the recombinant TTE0090 was successfully expressed and purified. The recombinant TTE0090 exhibited the catalysis of GLK, even at high temperatures. Detection of expression levels and catalysis of TTE0090 in vivo was furthermore carried out at different temperatures. The expression of TTE0090 was attenuated during the culture temperature elevated; however, the specific activity was positively correlated to temperature raised. This leads a possibility that the metabolic capacity of glycolysis in T. tencongensis is relatively constant at different temperatures. All the results herein demonstrate that TTE0090 is a novel gene of GLK. The studies on TTE0090 and its protein product, thus, may deepen our understanding of the adaptation mechanism of thermophilic bacteria living in harsh environment.
Qian
Zhong
Z
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101318, China. qianzhong@genomics.org.cn
Wang
Jing-Qiang
JQ
Zhou
Chuan-Qi
CQ
Ma
Yan-He
YH
Liu
Si-Qi
SQ
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D002384:Catalysis; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005941:Glucokinase / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D013696:Temperature; D046968:Thermoanaerobacter / Q000737:chemistry / Q000201:enzymology*
D001426:Bacterial Proteins; D005941:Glucokinase