Role of soluble triggering receptor expressed on myeloid cells in inflammatory bowel disease.
OBJECTIVE
To investigate the probable role of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in the pathogenesis of inflammatory bowel disease (IBD).


METHODS
Fifty-eight patients were enrolled; nineteen healthy volunteers served as controls; 8 patients were diagnosed with Crohn's disease, and 31 with ulcerative colitis. Clinical and endoscopic activity indexes of patients with Crohn's disease and ulcerative colitis respectively were estimated. Upon admission blood was sampled; sTREM-1 and TNFalpha were measured by an immunoassay and malondialdehyde (MDA) by the thiobarbitourate assay, after passage through an HPLC system.


RESULTS
Median +/- SE of TNFalpha of controls, patients with Crohn's disease and patients with ulcerative colitis were 6.02 +/- 3.94, 7.98 +/- 5.08 (P = NS vs controls), and 8.45 +/- 4.15 ng/L (P = 0.018 vs controls) respectively. Respective values of sTREM-1 were 53.31 +/- 32.93, 735.10 +/- 197.17 (P = 0.008 vs controls) and 435.82 +/- 279.71 ng/L (P = 0.049 vs controls). sTREM-1 was positively correlated with Crohn's disease activity index and clinical and endoscopic activity indexes of ulcerative colitis (P = 0.002, 0.001 and 0.009, respectively). sTREM-1 of patients with ulcerative colitis was positively correlated with TNFalpha (P = 0.001).


CONCLUSIONS
sTREM-1 seems to behave as a novel mediator in IBD in correlation with the degree of the inflammatory reaction of the intestinal mucosa.
Tzivras
Michalis
M
Department of Pathophysiology, Gastroenterology Section, University of Athens, Medical School, Greece.
Koussoulas
Vassilios
V
Giamarellos-Bourboulis
Evangelos J
EJ
Tzivras
Dimitrios
D
Tsaganos
Thomas
T
Koutoukas
Pantelis
P
Giamarellou
Helen
H
Archimandritis
Athanasios
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D003093:Colitis, Ulcerative / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D003424:Crohn Disease / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008562:Membrane Glycoproteins / Q000097:blood / Q000502:physiology*; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D011971:Receptors, Immunologic / Q000097:blood / Q000502:physiology*; D012720:Severity of Illness Index; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000502:physiology
D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde