PDGF isoforms induce and maintain anagen phase of murine hair follicles.
BACKGROUND
It is known that platelet-derived growth factor (PDGF) receptors are expressed in hair follicle (HF) epithelium.


OBJECTIVE
The aim of the present study was to clarify the effects of PDGF-AA and -BB on the cyclic growth of HFs.


METHODS
PDGF-AA or -BB was injected into the dorsal skin of C3H mice during the second telogen phase once daily for five consecutive days, or PDGF-AA or -BB dissolved in hyaluronic acid was injected only once. In order to confirm the effects of different PDGF isoforms, anti-PDGF-AA antibody or anti-PDGF-BB antibody was injected just after each injection of PDGF-AA or -BB. In addition, anti-PDGF antibodies were injected into the skin of C3H mice during the second anagen phase once daily for 5 days. We studied expression of signaling molecules in the skin where anagen phase had been induced by PDGF injection by real-time RT-PCR.


RESULTS
Both PDGF-AA and -BB injection experiments immediately induced the anagen phase of the hair growth cycle at the injection sites. The induction of anagen was interfered by anti-PDGF antibody treatment. Real-time RT-PCR using extracted RNA from the PDGF injected sites of skin samples showed upregulated expression of HF differentiation-related key signaling molecules, Sonic hedgehog (Shh), Lef-1 and Wnt5a.


CONCLUSIONS
These results indicate that both PDGF-AA and -BB are involved in the induction and maintenance of the anagen phase in the mouse hair cycle. Local application of PDGF-AA and -BB might therefore prove to be an effective treatment option for alopecia associated with early catagen induction and elongated telogen phase.
Tomita
Y
Y
Department of Dermatology, Hokkaido University Graduate School of Medicine, N15 W7 Sapporo 060-8638, Japan.
Akiyama
M
M
Shimizu
H
H
D000818:Animals; D000077214:Becaplermin; D005786:Gene Expression Regulation / Q000187:drug effects; D006197:Hair / Q000254:growth & development*; D018859:Hair Follicle / Q000187:drug effects / Q000502:physiology*; D053823:Hedgehog Proteins; D006801:Humans; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000235:genetics; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000187:drug effects; D015534:Trans-Activators / Q000235:genetics
D053823:Hedgehog Proteins; C497574:Lef1 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; C509239:SHH protein, human; D015534:Trans-Activators; C075278:platelet-derived growth factor A; D000077214:Becaplermin