Coordination of nuclear- and mitochondrial-DNA encoded proteins in cancer and normal colon tissues.
To support the rapid growth of tumors, the cell can respond by increasing the number of mitochondria, in a concerted biosynthesis of mitochondrial constituents (nuclear and mitochondria encoded). Increased transcription, availability and stability of oxidative phosphorylation mRNAs, without increasing mitochondria number could also lead to more rapid energy-yielding effects. Mitochondria biogenesis and de novo formation of respiratory chain components imply coordination of nuclear and mt gene transcription. The mitochondrial mass is regulated by a number of physiopathological conditions. In response to external stimuli, mitochondria biogenesis is dependent on an orchestrated crosstalk between the nuclear and the mitochondrial genomes. Based on the higher incidence of glycolysis over oxidative phosphorylation in cancer tissues, we studied by differential proteomics the energy metabolism pathway of matched samples of normal and cancer tissue. Our results indicated that oxidative phosphorylation in cancer cells seemed altered because there is an unbalanced coordination between nuclear- and mitochondria-encoded mitochondrial proteins.
Mazzanti
Roberto
R
Department Internal Medicine, University of Florence, Florence, Italy.
Giulivi
Cecilia
C
D000230:Adenocarcinoma / Q000378:metabolism*; D000368:Aged; D000369:Aged, 80 and over; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D004247:DNA / Q000502:physiology*; D004272:DNA, Mitochondrial / Q000502:physiology; D004273:DNA, Neoplasm / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D010085:Oxidative Phosphorylation; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D012007:Rectum / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm; D024101:Mitochondrial Proteins; D009363:Neoplasm Proteins; D020543:Proteome; D014157:Transcription Factors; D004247:DNA