title: [Lipophilic toxin profiles associated with diarrhetic shellfish poisoning in scallops, Patinopecten yessoensis, collected in Hokkaido and comparison of the quantitative results between LC/MS and mouse bioassay].
abstract: Lipophilic toxins associated with diarrhetic shellfish poisoning (DSP) in scallops, Patinopecten yessoensis, collected in Hokkaido, Japan were quantified by liquid chromatography-mass spectrometry (LC/MS). Pectenotoxin-6 (PTX6) and yessotoxin (YTX) were the dominant toxins in the scallops, although the percentages of these toxins were different depending on the production area or the sampling period. The quantitative results obtained for the scallops in LC/MS and in mouse bioassay (MBA) were compared. Fifty of the 55 samples found to be exceeding the local quarantine level (0.025 MU/g whole meat) in Hokkaido by LC/MS were quantified by MBA as being below the quarantine level. It is suggested that this discrepancy is due to poor detection of YTX by MBA. These results indicate that LC/MS is a better method than MBA in terms of sensitivity and accuracy to quantify known lipophilic toxins, including YTX.
authors.affiliation: Hokkaido Institute of Public Health, Sapporo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yuri
authors.initials: Y
authors.lastname: Shirota
authors.forename: Motohisa
authors.initials: M
authors.lastname: Honma
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Itabashi
authors.forename: Takao
authors.initials: T
authors.lastname: Chyounan
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamiyama
mesh_terms: D000818:Animals; D001681:Biological Assay; D002853:Chromatography, Liquid; D004988:Ethers, Cyclic / Q000032:analysis; D005517:Foodborne Diseases / Q000209:etiology*; D007564:Japan; D013058:Mass Spectrometry; D051379:Mice; D008978:Mollusk Venoms / Q000032:analysis*; D035964:Oxocins / Q000032:analysis; D049897:Pectinidae / Q000737:chemistry*
chemical_list: D004988:Ethers, Cyclic; D008978:Mollusk Venoms; D035964:Oxocins; C066632:yessotoxin