Fine mapping of the keratoconus with cataract locus on chromosome 15q and candidate gene analysis.
OBJECTIVE
To report the fine mapping of the keratoconus with cataract locus on chromosome 15q and the mutational analysis of positional candidate genes.


METHODS
Genotyping of two novel microsatellite markers and a single nucleotide polymorphism (SNP) in the critical region of linkage for keratoconus with cataract on 15q was performed. Positional candidate genes (MORF4L1, KIAA1055, ETFA, AWP1, REC14, KIAA1199, RCN2, FAH, IDH3A, MTHFS, ADAMTS7, MAN2C1, PTPN9, KIAA1024, ARNT2, BCL2A1, ISL2, C15ORF22 (P24B), DNAJA4, FLJ14594, CIB2 (KIP2), C15ORF5, and PSMA4) prioritized on the basis of ocular expression and probable function were screened by PCR-based DNA sequencing methods.


RESULTS
We report the refinement of the linkage region for keratoconus with cataract to an interval of approximately 5.5 Mb flanked by the MAN2C1 gene and the D15S211 marker on chromosome 15q. Mutational analysis of positional candidate genes detected many sequence variations and single nucleotide polymorphisms. None of the sequence variants were considered pathogenic as they were also found in unaffected family members and normal control DNA samples.


CONCLUSIONS
Fine mapping of the keratoconus with cataract locus on 15q has reduced the linked region to 5.5 Mb, thereby excluding 28 candidate genes. A further 23 candidate genes were excluded by direct sequencing methods, although a pathogenic genomic rearrangement or exonic deletion would not have been detected.
Dash
Durga Prasad
DP
Department of Medical Genetics, Queen's University of Belfast, Royal Victoria Hospital, Belfast, United Kingdom. d.dash@queens-belfast.ac.uk
Silvestri
Giuliana
G
Hughes
Anne E
AE
D016022:Case-Control Studies; D002386:Cataract / Q000150:complications* / Q000235:genetics*; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D004252:DNA Mutational Analysis; D008040:Genetic Linkage; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D007640:Keratoconus / Q000150:complications* / Q000235:genetics*; D008361:Mannosidases / Q000235:genetics / Q000378:metabolism; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D043323:alpha-Mannosidase
D007527:Isoenzymes; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; C511245:MAN2C1 protein, human; D043323:alpha-Mannosidase