[Transfection of pemt-2-cDNA inhibits the expression of cell cycle related proteins in rat CBRH-7919 hepatoma cells].
OBJECTIVE
To unravel the molecular mechanism of proliferation inhibition induced by transfection of pemt2-cDNA into rat CBRH-7919 hepatoma cells.


METHODS
We started with the highly expressed PEMT2 clone. Cell culture and Western blotting techniques were used to examine the expression of cyclinD1/CDK4, cyclinE/CDK2, phospho-Rb, caspase-3, c-jun and caveolins.


RESULTS
Our results showed that CDK4, CDK2, phospho-Rb and c-jun were down regulated in the pemt2 highly expressed cell clone. The high expression clone of pemt2-transfected cells also showed over expression of caspase-3.


CONCLUSIONS
The reductions of proliferation and apoptosis of pemt2 transfected cells could be related to the G1 phase arrest induced by down-regulation of the cell cycle-associated proteins.
Liu
Cui-ping
CP
Department of Biology, Liaoning Normal University, Dalian 116029, China.
Zou
Wei
W
Wang
Liang
L
Cui
Zhao-chun
ZC
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D053148:Caspase 3 / Q000096:biosynthesis / Q000235:genetics; D051357:Cyclin-Dependent Kinase 2 / Q000096:biosynthesis* / Q000235:genetics; D051358:Cyclin-Dependent Kinase 4 / Q000096:biosynthesis* / Q000235:genetics; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis / Q000235:genetics; D015536:Down-Regulation; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism*; D050918:Phosphatidylethanolamine N-Methyltransferase / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D050918:Phosphatidylethanolamine N-Methyltransferase; C495894:CDK2 protein, human; C495900:CDK4 protein, human; D051357:Cyclin-Dependent Kinase 2; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases; D053148:Caspase 3