title: The effect of sucrose and trehalose on viability of one- and two-cell rabbit embryos.
abstract: The effect of sucrose and trehalose on the viability of one- and two-cell rabbit embryos was investigated. A significant decrease in the viability of one- and two-cell embryos exposed for 30 min. at 20 degrees C was observed. At 38 degrees C none of the two-cell embryos in a sucrose solution survived after 30 min exposure, while approximately 50% of the embryos survived in a trehalose solution. The cleveage rate in culture of two-cell embryos exposed both to 2.0 M or 1.45 M trehalose was significantly lower in comparison with the control group. However the survival rate after transfer of two-cell embryos exposed to 1.45 M trehalose solution at 20 degrees C remained the same as that of the control group.
authors.affiliation: Institute of Zootechnics Department of Animal Reproduction and AI 32-083 Balice/Krakow, Poland.
authors.forename: Z
authors.initials: Z
authors.lastname: Smorag
authors.forename: Y
authors.initials: Y
authors.lastname: Heyman
authors.forename: V
authors.initials: V
authors.lastname: Garnier
authors.forename: B
authors.initials: B
authors.lastname: Gajda