In vitro monitoring picogram roxithromycin in human urine using flow injection chemiluminescence procedure.
A sensitive chemiluminescence method, based on the enhancive effect of roxithromycin on the chemiluminescence reaction between luminol and hydrogen peroxide in a flow injection system, was proposed for the determination of roxithromycin. The increment of chemiluminescence intensity was linear with roxithromycin concentration in the range 1.0-1000 pg ml(-1) with the detection limit of 0.3 pg ml(-1) (3sigma). At a flow rate of 2.0 ml min(-1), a complete analytical process could be performed within 0.5 min, including sampling and washing, with a relative standard deviation of less than 5%. The proposed procedure was applied successfully in the monitoring of roxithromycin in human urine without any pre-treatment procedures and it was found that roxithromycin in urine reached its maximum after orally administrated for two hours, presenting an excretive ratio of 4.6% in 12 h. With urinary excretion rate method, the total elimination rate constant k and half-life time t(1/2) of roxithomycin was calculated, which was 0.1831, 3.785 h.
Song
Zhenghua
Z
Department of Chemistry, Northwest University, Xi'an 710069, China. songzhenghua@hotmail.com
Liu
Yanhong
Y
Xie
Xiaofeng
X
D000900:Anti-Bacterial Agents / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine*; D005260:Female; D017022:Flow Injection Analysis; D006801:Humans; D006861:Hydrogen Peroxide; D008163:Luminescent Measurements; D008165:Luminol; D015575:Roxithromycin / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine*; D012972:Sodium Hydroxide
D000900:Anti-Bacterial Agents; D015575:Roxithromycin; D012972:Sodium Hydroxide; D008165:Luminol; D006861:Hydrogen Peroxide