Interactions between model membranes and lignin-related compounds studied by immobilized liposome chromatography.
In order to elucidate the modes of interaction between lignin precursors and membranes, we have studied the influence of temperature, lipid composition and buffer composition on the partitioning of monolignol and dilignol model substances into phospholipid bilayers. The partitioning was determined by immobilized liposome chromatography, which is an established method for studies of pharmaceutical drugs but a new approach in studies of lignin synthesis. The temperature dependence of the retention and the effect of a high ammonium sulfate concentration in the mobile phase demonstrated that the interaction involved both hydrophobic effects and polar interactions. There was also a good correlation between the partitioning and the estimated hydrophobicity, in terms of octanol/water partitioning. The partitioning behavior of the model substances suggests that passive diffusion over the cell membrane is a possible transport route for lignin precursors. This conclusion is strengthened by comparison of the present results with the partitioning of pharmaceutical drugs that are known to pass cell membranes by diffusion.
Boija
Elisabet
E
Department of Biochemistry and Organic Chemistry, Biomedical Center, Uppsala University, Box 576, SE-751 23 Uppsala, Sweden.
Johansson
Gunnar
G
D002853:Chromatography, Liquid / Q000379:methods*; D008081:Liposomes; D008567:Membranes, Artificial; D008962:Models, Theoretical; D013816:Thermodynamics
D008081:Liposomes; D008567:Membranes, Artificial