title: Evidence of association of macrophage migration inhibitory factor gene polymorphisms with systemic lupus erythematosus.
abstract: The aim of this study was to evaluate the potential association of functional polymorphisms of macrophage migration inhibitory factor with systemic lupus erythematosus. Our study includes 711 systemic lupus erythematosus (SLE) patients and 755 healthy controls. We genotyped the migration inhibitory factor (MIF) -173G/C using a polymerase chain reaction (PCR) system with predeveloped TaqMan allelic discrimination assay and the MIF -794 CATT(n) microsatellite polymorphism using a PCR-fluorescent method. A statistically significant difference in the distribution of the MIF -173(*)C allele between SLE patients and controls (P=0.004, OR=1.34, 95% CI=1.05-1.27) was observed. In addition, the frequency of the MIF -173(*)C/C genotype was higher in SLE patient (P=0.002, OR=2.58, 95% CI=1.32-5.10). No differences in the distribution of CATT(n) were found. However, the haplotypes analyses showed that only the CATT(7)-MIF -173(*)C haplotype was associated with a higher susceptibility to SLE (P=0.001, OR 1.84, 95% CI 1.35-2.79). No association with clinical features was detected in any case. These results suggest that both, MIF -173(*)C allele and CATT(7)-MIF -173(*)C haplotype, confer susceptibility to SLE in our population.
authors.affiliation: Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Granada, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Sánchez
authors.forename: L M
authors.initials: LM
authors.lastname: Gómez
authors.forename: M A
authors.initials: MA
authors.lastname: Lopez-Nevot
authors.forename: M A
authors.initials: MA
authors.lastname: González-Gay
authors.forename: J M
authors.initials: JM
authors.lastname: Sabio
authors.forename: N
authors.initials: N
authors.lastname: Ortego-Centeno
authors.forename: E
authors.initials: E
authors.lastname: de Ramón
authors.forename: J M
authors.initials: JM
authors.lastname: Anaya
authors.forename: M F
authors.initials: MF
authors.lastname: González-Escribano
authors.forename: B P
authors.initials: BP
authors.lastname: Koeleman
authors.forename: J
authors.initials: J
authors.lastname: Martín
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D016001:Confidence Intervals; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000097:blood / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D044465:White People
chemical_list: D008263:Macrophage Migration-Inhibitory Factors