title: Cell-type specific proximity of centromeric domains of one homologue each of chromosomes 2 and 11 in nuclei of cerebellar Purkinje neurons.
abstract: In Purkinje neurons of the mouse cerebellum, the centromeres of several chromosomes are placed in close proximity to form a distinct pattern of clusters and exhibit reproducible spatial redistributions during development. In granule neurons, an adjacent cell type in the cerebellum, the pattern, size, and number of centromeric aggregations are different from those of Purkinje neurons. The present work was undertaken to test the hypothesis that the same chromosomes form part of one aggregate in a cell-type-specific manner. Fluorescence in situ hybridization (FISH) with chromosome-specific paracentromeric probes was used to identify centromeric regions of individual chromosomes in cerebellar Purkinje and granule neurons of the adult mouse. When pairs of centromeric probes were used in two-color FISH, one homologue each of chromosomes 2 and 11 were routinely found close to each other in Purkinje neurons but not in granule neurons. This finding of specific proximity was limited to the pair 2 and 11, out of the ten chromosome pairs that were randomly selected and studied. Our results indicate that, in adult Purkinje neurons, a cell-type-specific spatial proximity is present between centromeric domains of one homologue each of chromosomes 2 and 11.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Toronto, M5S 1A8 Toronto, ON, Canada.
authors.forename: Kunjumon I
authors.initials: KI
authors.lastname: Vadakkan
authors.forename: Baoxiang
authors.initials: B
authors.lastname: Li
authors.forename: Umberto
authors.initials: U
authors.lastname: De Boni
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics / Q000648:ultrastructure; D002503:Centromere / Q000235:genetics* / Q000648:ultrastructure*; D015342:DNA Probes / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D011689:Purkinje Cells / Q000648:ultrastructure*
chemical_list: D015342:DNA Probes