HIV-1 Nef is associated with complex pulmonary vascular lesions in SHIV-nef-infected macaques.
BACKGROUND
HIV-infected patients with pulmonary arterial hypertension have histologic manifestations that are indistinguishable from those found in patients with idiopathic pulmonary arterial hypertension. In addition, the role of pleiotropic viral proteins in the development of plexiform lesions in HIV-related pulmonary hypertension (HRPH) has not been explored. Simian immunodeficiency virus (SIV) infection of macaques has been found to closely recapitulate many of the characteristic features of HIV infection, and thus hallmarks of pulmonary arterial hypertension should also be found in this nonhuman primate model of HIV.


OBJECTIVE
To determine whether pulmonary arterial lesions were present in archived SIV-infected macaque lung tissues from Johns Hopkins University and two National Primate Research Centers.


METHODS
Archived macaque and human lung sections were examined via immunohistochemistry for evidence of complex vascular lesions.


RESULTS
Complex plexiform-like lesions characterized by lumenal obliteration, intimal disruption, medial hypertrophy, thrombosis, and recanalized lumena were found exclusively in animals infected with SHIV-nef (a chimeric viral construct containing the HIV nef gene in an SIV backbone), but not in animals infected with SIV. The mass of cells in the lesions were factor VIII positive, and contained cells positive for muscle-specific and smooth muscle actins. Lung mononuclear cells were positive for HIV Nef, suggesting viral replication. Endothelial cells in both the SHIV-nef macaques and patients with HRPH, but not in patients with idiopathic pulmonary arterial hypertension, were also Nef positive.


CONCLUSIONS
The discovery of complex vascular lesions in SHIV-nef- but not SIV-infected animals, and the presence of Nef in the vascular cells of patients with HRPH, suggest that Nef plays a key role in the development of severe pulmonary arterial disease.
Marecki
John C
JC
Department of Medicine, University of Colorado at Denver and Health Sciences Center, 4200 East Ninth Avenue, Box C272, Denver, CO 80262, USA. John.Marecki@UCHSC.edu
Cool
Carlyne D
CD
Parr
Jane E
JE
Beckey
Virginia E
VE
Luciw
Paul A
PA
Tarantal
Alice F
AF
Carville
Angela
A
Shannon
Richard P
RP
Cota-Gomez
Adela
A
Tuder
Rubin M
RM
Voelkel
Norbert F
NF
Flores
Sonia C
SC
D000818:Animals; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015693:Gene Products, nef / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D006976:Hypertension, Pulmonary / Q000235:genetics*; D007150:Immunohistochemistry; D008251:Macaca; D016097:Simian Acquired Immunodeficiency Syndrome / Q000235:genetics*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000472:pathogenicity; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics*; D014779:Virus Replication; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; C429284:NEF protein, SIV; D054334:Viral Regulatory and Accessory Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus