title: [Biological significance of E-cadherin in an inflammatory breast carcinoma cell line].
abstract: OBJECTIVE
  To investigate the effects of E-cadherin on the biologic behavior of SUM149, an inflammatory breast cancer cell line.
  
  
  METHODS
  SUM149 cells were transfected with dominant-negative mutant E-cadherin expressing plasmid. The positive clones with higher expression of dominant-negative E-cadherin mutant were identified by RT-PCR and fluorescent flow cytometry method. The differences in cell growth, proliferation and invasion between positive clones and controls were compared.
  
  
  RESULTS
  Whereas the proliferation of positive clones was of no change, compared with controls, the ability of invasion was decreased and the mRNA levels of MMP-1 and MMP-9 were downregulated. Gelatin zymography analysis also confirmed the decreasing expression of MMP-9 in the positive clones.
  
  
  CONCLUSIONS
  In this cell line model, down-regulation of E-cadherin can inhibit the ability of invasion of this inflammatory breast cancer cell line.
authors.affiliation: Department of Breast Surgery, Cancer Institute, Shanghai Cancer Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Hui-ming
authors.initials: HM
authors.lastname: Dong
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Jiong
authors.initials: J
authors.lastname: Wu
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Lu
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Luo
authors.forename: Zhen-zhou
authors.initials: ZZ
authors.lastname: Shen
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Shao
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D009154:Mutation; D009361:Neoplasm Invasiveness; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
chemical_list: D015820:Cadherins; D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1