Recombinant expression of Toluene o-Xylene Monooxygenase (ToMO) from Pseudomonas stutzeri OX1 in the marine Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.
The psychrophilic bacterium Pseudoalteromonas haloplanktis TAC125, isolated from Antarctic seawater, was used as recipient for a biodegradative gene of the mesophilic Pseudomonas stutzeri OX1. tou cluster, coding for Toluene o-Xylene Monooxygenase (ToMO), was successfully cloned and expressed into a "cold expression" vector. Apparent catalytic parameters of the recombinant microorganisms on three different substrates were determined and compared with those exhibited by Escherichia coli recombinant cells expressing ToMO. Production of a catalytically efficient TAC/tou microorganism supports the possibility of developing specific degradative capabilities for the bioremediation of chemically contaminated marine environments and of industrial effluents characterised by low temperatures.
Siani
Loredana
L
Dipartimento di Chimica Organica e Biochimica, Complesso Universitario Monte S. Angelo, Via Cinthia, 80126 Napoli, Italy.
Papa
Rosanna
R
Di Donato
Alberto
A
Sannia
Giovanni
G
D000864:Antarctic Regions; D015870:Gene Expression / Q000502:physiology; D008386:Marine Biology; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D031161:Pseudoalteromonas / Q000201:enzymology* / Q000235:genetics; D044223:Pseudomonas stutzeri / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity
D011994:Recombinant Proteins; D010105:Oxygenases; C114360:toluene 2-xylene monooxygenase