title: In situ replication of immediate dendritic cell (DC) precursors contributes to conventional DC homeostasis in lymphoid tissue.
abstract: The developmental biology of dendritic cells (DC) under physiological conditions remains unclear. In this study, we show that mouse CD11c(+) MHC class II(-)lineage(-) cells are immediate precursors of conventional DC and are widely distributed in both bone marrow and lymphoid tissues. These precursors have a high clonal efficiency, and when cocultured on a supportive stromal monolayer or adoptively transferred in vivo, generate a population CD11c(+)MHC class II(+) DC that retain limited proliferation capacity. During steady state conditions, a small proportion of immediate DC precursors (DCp) and DCs are dividing actively in bone marrow and spleen. Cytokines that initiate and support proliferation of immediate DCp were defined. Collectively, our findings provide evidence of a distinct development pathway for conventional DC in both bone marrow and lymphoid tissues and highlight the importance of in situ replication of immediate DCp and DC in maintaining conventional DC populations.
authors.affiliation: Multiorgan Transplantation Program, Toronto General Research Institute, Toronto General Hospital, Ontario, Canada
authors.forename: Jun
authors.initials: J
authors.lastname: Diao
authors.forename: Erin
authors.initials: E
authors.lastname: Winter
authors.forename: Claude
authors.initials: C
authors.lastname: Cantin
authors.forename: Wenhao
authors.initials: W
authors.lastname: Chen
authors.forename: Luoling
authors.initials: L
authors.lastname: Xu
authors.forename: David
authors.initials: D
authors.lastname: Kelvin
authors.forename: James
authors.initials: J
authors.lastname: Phillips
authors.forename: Mark S
authors.initials: MS
authors.lastname: Cattral
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D002455:Cell Division / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002999:Clone Cells; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D006706:Homeostasis / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015398:Signal Transduction / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism
chemical_list: D017493:Leukocyte Common Antigens