Influence of organochlorine pesticides on physiological potency of cultured granulosa cells from bovine preovulatory follicles.
In the present study the direct effect of Dichlorodiphenyltrichloroethane (DDT), Methoxychlor (MXC) and Hexachlorocyclohexane (gammaHCH) was investigated on the DNA synthesis (measured by [3H]thymidine incorporation); proliferation (determined by colorimetric MTT[3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide] assay); and steroidogenesis (estradiol 17beta, progesterone determination) of granulosa cells during in vitro culture. The cells were aspirated from preovulatory follicles in PMSG-treated heifers. They were cultivated in phenol red and serum (0.1% FCS) RPMI 1640 medium without or with the 3 pesticides in different concentrations. In summary, the results showed differential inhibition of parameters named above by these pesticides (gammaHCH < MXC < DDT) in comparison with vehicle-treated control. Progesterone synthesis was more inhibited than estradiol 17beta production by 3 chemicals, which resulted from the loss of viability of cells indicated by estimation of cell-shape-factor and viability of cells. Besides the inhibitory effect, there was a stimulatory effect of low concentrations of DDT and MXC on [3H]thymidine incorporation and proliferation.
Tiemann
U
U
Department of Reproductive Biology, Research Institute for Biology of Farm Animals, Dummerstorf, Germany.
Pöhland
R
R
Schneider
F
F