Silencing of HIV by hairpin-loop-structured DNA oligonucleotide.
We describe inhibition of HIV replication by a partially double-stranded 54mer oligodeoxynucleotide, ODN, which consists of an antisense strand targeting the highly conserved polypurine tract, PPT, of HIV, and a second strand, compatible with triple-helix formation. Upon treatment of HIV-infected cells with ODN early after infection no viral nucleic acids, syncytia or p24 viral antigen expression was observed. The ODN-mediated effect was highly sequence-specific. The ODN against HIV-IIIB was effective preferentially against its homologous PPT and less against the PPT of HIV-BaL differing in two of 24 nucleotides and vice versa. It may be interesting mechanistically as an antiviral drug.
Moelling
Karin
K
Institute of Medical Virology, University of Zurich, Switzerland. moelling@immv.unizh.ch
Abels
Susanne
S
Jendis
Joerg
J
Matskevich
Alexey
A
Heinrich
Jochen
J
D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D020868:Gene Silencing; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication
D017931:DNA Primers; D004279:DNA, Viral