Covalent microcontact printing of proteins for cell patterning.
We describe a straightforward approach to the covalent immobilization of cytophilic proteins by microcontact printing, which can be used to pattern cells on substrates. Cytophilic proteins are printed in micropatterns on reactive self-assembled monolayers by using imine chemistry. An aldehyde-terminated monolayer on glass or on gold was obtained by the reaction between an amino-terminated monolayer and terephthaldialdehyde. The aldehyde monolayer was employed as a substrate for the direct microcontact printing of bioengineered, collagen-like proteins by using an oxidized poly(dimethylsiloxane) (PDMS) stamp. After immobilization of the proteins into adhesive "islands", the remaining areas were blocked with amino-poly(ethylene glycol), which forms a layer that is resistant to cell adhesion. Human malignant carcinoma (HeLa) cells were seeded and incubated onto the patterned substrate. It was found that these cells adhere to and spread selectively on the protein islands, and avoid the poly(ethylene glycol) (PEG) zones. These findings illustrate the importance of microcontact printing as a method for positioning proteins at surfaces and demonstrate the scope of controlled surface chemistry to direct cell adhesion.
Rozkiewicz
Dorota I
DI
Laboratory of Supramolecular Chemistry and Technology, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Kraan
Yvonne
Y
Werten
Marc W T
MW
de Wolf
Frits A
FA
Subramaniam
Vinod
V
Ravoo
Bart Jan
BJ
Reinhoudt
David N
DN
D000447:Aldehydes / Q000737:chemistry; D002448:Cell Adhesion; D024061:Collagen Type III / Q000737:chemistry* / Q000378:metabolism; D003584:Cytological Techniques / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007097:Imines / Q000737:chemistry; D018613:Microscopy, Confocal; D011092:Polyethylene Glycols / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012828:Silicones / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D000447:Aldehydes; C439971:COL3A1 protein, human; D024061:Collagen Type III; D004129:Dimethylpolysiloxanes; D007097:Imines; D012828:Silicones; D011092:Polyethylene Glycols; C013830:baysilon; D006046:Gold; D012822:Silicon Dioxide