Two-generation reproduction toxicity study in rats with 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p'-DDT).
DDT, an organochlorine pesticide, has been cited as a representative chemical suspected of having endocrine disrupting effects. In this study, the potential endocrine disrupting activities of p,p'-DDT, a major component of DDT, were investigated in rats in a 2-generation reproduction toxicity study in accordance with the most current test guidelines of the Ministry of Agriculture, Forestry and Fisheries in Japan, Organization for Economic Cooperation and Development (OECD) and United States Environmental Protection Agency (USEPA) with some modifications and additions. p,p'-DDT was given to parental rats at dietary levels of 0, 5, 50 or 350 ppm. Systemic toxicities in the parental animals consisted of tremors and subsequent deaths (females only) and/or pathological alterations of the liver (both sexes of animals) of the 2 higher dose groups. Reproductive and postnatal developmental toxicities were not evident up to the highest dose level except for the decreased pup viability index on postnatal day 21 in the 350 ppm group. Changes in serum estradiol and progesterone levels and/or a delay in male sexual maturation were noted in the 2 higher dose groups in a dose-dependent fashion, suggesting alterations of endogenous endocrine functions. However, these changes never resulted in substantial reproductive disorders.
Hojo
Hitoshi
H
Laboratories of Reproductive Toxicology, Toxicology Division, Institute of Environmental Toxicology, Ibaraki, Japan.
Aoyama
Hiroaki
H
Takahashi
Ken L
KL
Shimizu
Naoko
N
Araki
Masayuki
M
Takizawa
Yoshio
Y
Sakasai
Koji
K
Kuwahara
Maki
M
Saka
Machiko
M
Teramoto
Shoji
S
D000014:Abnormalities, Drug-Induced; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003634:DDT / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000097:blood; D004967:Estrogens / Q000378:metabolism; D005260:Female; D008099:Liver / Q000187:drug effects; D008297:Male; D010575:Pesticides / Q000633:toxicity*; D011247:Pregnancy; D011270:Pregnancy, Animal; D011374:Progesterone / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
D004967:Estrogens; D010575:Pesticides; D011374:Progesterone; D004958:Estradiol; D003634:DDT