title: Effect of AgK114 on picryl chloride-induced chronic contact hypersensitivity responses.
abstract: BACKGROUND
  Mouse AgK114 (a glycosylphosphatidylinositol (GPI) anchored membrane-associated protein) expression is found in somatotrophs of the pituitary gland in correlation with the expression of growth hormone. In this study, the effects of AgK114 on the systemic immune response were examined in contact hypersensitivity (CHS) model mice.
  
  
  METHODS
  AgK114 was intraperitoneally injected into BALB/c mice that were sensitized and challenged with picryl chloride (PiCl). Serum IgE levels and the antigen-specific cytokine production by lymph node (LN) cells were examined.
  
  
  RESULTS
  The serum IgE levels in the CHS mice treated with 10 microg/head of AgK114 during the repeated challenge with PiCl were significantly decreased compared with those of the control mice. Moreover, IL-4 production by LN cells in response to 2,4,6-trinitrobenzene-sulfonic acid sodium salt-treated splenocytes was decreased in the AgK114-treated CHS mice compared with that of the control mice.
  
  
  CONCLUSIONS
  Our results suggest that systemic administration of AgK114 exerted immunoregulatory functions on the allergic responses, resulting in the inhibition of IgE production.
authors.affiliation: Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., 675-1 Fujisaki, Okayama 702-8006, Japan. fujisaki@hayashibara.co.jp
authors.forename: Chikako
authors.initials: C
authors.lastname: Arai
authors.forename: Tomoki
authors.initials: T
authors.lastname: Tatefuji
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mori
authors.forename: Akiko
authors.initials: A
authors.lastname: Mizote
authors.forename: Keizo
authors.initials: K
authors.lastname: Kohno
authors.forename: Tsunetaka
authors.initials: T
authors.lastname: Ohta
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002908:Chronic Disease; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy* / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D017261:Glycosylphosphatidylinositols / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000097:blood; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007371:Interferon-gamma / Q000032:analysis; D016753:Interleukin-10 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D010853:Picryl Chloride / Q000633:toxicity*; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014302:Trinitrobenzenesulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D017261:Glycosylphosphatidylinositols; D011994:Recombinant Proteins; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D014302:Trinitrobenzenesulfonic Acid; D010853:Picryl Chloride