title: Embryo transfer as a means of controlling viral infections. VI. Bluetongue virus-free calves from infectious semen.
abstract: Four Holstein heifers were superovulated and inseminated with infectious semen from a bull experimentally infected with type 17 bluetongue virus (BTV). A total of 20 embryos were collected at donor slaughter and transferred to 16 recipients. Ten recipients became pregnant of which one subsequently aborted, one gave birth to twins which died at birth, one was killed at term because of dystocia, and 7 gave birth to live calves one of which died perinatally. All animals were tested for BTV antibodies at the time of slaughter which was at least 30 days post partum for surviving heifers and calves. Two of the four donor heifers were retrospectively determined to have been infected by the semen (viremia demonstrated) and their embryos accounted for 9 of the 10 pregnancies including the six surviving calves. None of the recipients or calves developed BTV antibody by the termination of the experiment. This study suggests that BTV-free calves can be readily obtained from the use of BTV-positive semen.
authors.affiliation: Agriculture Canada, Animal Diseases Research Institute, NEPEAN, P.O. Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9.
authors.forename: F C
authors.initials: FC
authors.lastname: Thomas
authors.forename: E L
authors.initials: EL
authors.lastname: Singh
authors.forename: W C
authors.initials: WC
authors.lastname: Hare