title: [The expression and significance of eotaxin gene in nasal mucosa and CCR3 in bone marrow in model of allergic rhinitis].
abstract: OBJECTIVE
  To study the expression and significance of Eotaxin gene in nasal mucosa and CCR3 in bone marrow in model of allergic rhinitis.
  
  
  METHODS
  Twenty SD rats were randomly divided into allergic rhinitis (AR) group and normal control group. Ovalbumin (OVA) sensitized and challenged rats were used as the model of allergic rhinitis. The leucocytes in the smears of bone marrow and peripheral blood were counted, and the expression of CCR3 in bone marrow was detected by immunohistochemistry. The pathologic samples of nasal mucosa were stained with HE. The expression of Eotaxin mRNA in nasal mucosa was detected by in situ hybridization (ISH), and the expression of Eotaxin in nasal mucosa was detected by immunohistochemistry.
  
  
  RESULTS
  The ratio of eosinophils to white cells in bone marrow smears of AR group was significantly higher than that of normal control group (P < 0.01). The ratio of eosinophils to white cells in peripheral bloods mears of AR group was significantly higher than that of normal control group (P < 0.01). Eotaxin positive cell and the expression of Eotaxin mRNA in nasal mucosa of AR group were significantly higher than that of normal control group (P < 0.01). The expression of Eotaxin mRNA significantly positively correlated with the expression of Eotaxin (r = 0.804, P < 0.01). The number of Eotaxin positive cells in nasal mucosa was significantly positively correlated with the number of eosinophils in nasal mucosa of AR group (r = 0.795, P < 0.01). The ratio of CCR3 positive cells to white cells in bone marrow smears of AR group was significantly higher than that of normal control group (P < 0.01). The ratio of CCR3 positive cells to white cells was significantly positively correlated with the ratio of eosinophils to white cells in bone marrow smears of AR group (r = 0.736, P < 0.05).
  
  
  CONCLUSIONS
  The expression of Eotaxin and Eotaxin mRNA in nasal mucosa increased, and the expression of CCR3 in bone marrow increased in the model of allergic rhinitis. It supplies a cooperation between CCR3 and Eotaxin to allow rapid mobilization of a pool of bone marrow eosinophils followed by recruitment to the nasal mucosa.
authors.affiliation: Department of Otorhinolaryngology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China. zmh0334@yahoo.com.cn
authors.forename: Minghui
authors.initials: M
authors.lastname: Zhou
authors.forename: Mingmin
authors.initials: M
authors.lastname: Dong
authors.forename: Yuying
authors.initials: Y
authors.lastname: Wu
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Baohong
authors.initials: B
authors.lastname: Yue
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D054413:Chemokine CCL11 / Q000378:metabolism*; D005260:Female; D009297:Nasal Mucosa / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D054397:Receptors, CCR3 / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism* / Q000473:pathology
chemical_list: C515458:Ccl11 protein, rat; C515352:Ccr3 protein, rat; D054413:Chemokine CCL11; D054397:Receptors, CCR3