Effect of porcine reproductive and respiratory syndrome virus (PRRSV) on development of porcine fertilized ova in vitro.
Fertilized pig ova, collected from 14 estrus-synchronized gilts 32 h after presumed ovulation were microinjected or cultured for 72 h in Beltsville Embryo Culture Medium-3 with or without Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). To detect virus in the samples, virus isolation of swine alveolar macrophage, Reverse Transcriptase Polymerase Chain Reaction and Fluorescent Antibody techniques were employed. Microinjection or incubation of embryos with PRRSV did not significantly inhibit development of the porcine embryos in vitro when compared with that of controls (P = 0.75 and P = 0.14, respectively). Although either 10 to 20 TCID50 were microinjected or large concentrations of virus were used for embryo exposure by incubation, PRRSV was not detected in association with the embryos. It is concluded based on the experiments reported here that 4- to 16-cell stage pig embryos are not susceptible to productive infection with PRRSV in vitro.
Prieto
C
C
Departamento de Patología Animal I, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain.
Suárez
P
P
Martín-Rillo
S
S
Simarro
I
I
Solana
A
A
Castro
J M
JM