Spectroscopic studies of stratum corneum model membrane from Bothrops jararaca treated with cationic surfactant.
This research employed FT-Raman and PAS-FTIR spectroscopic techniques to evaluate the interaction of cetyl trimethyl ammonium chloride (CTAC), a cationic surfactant, on the stratum corneum (SC) of shed snake skins from Bothrops jararaca, used as model membranes. Surfactant aqueous solutions (50.0 and 0.78 gl(-1)) with neutral pH were applied on the samples with intervals of 4, 8 (whole SC) and 12h (SC tape-stripped). Samples presented modifications of the topography for all conditions of the assays and the monomers of the surfactant, instead of the micelles, seemed to interact with the keratin. The SC model membranes treated with CTAC have had an augment of water content (except for whole SC treated for 8h) indicated by the expansion of the band 3600-3300 cm(-1), mainly for the tape-stripped samples after 12h treatment. Concentration appeared to be an important factor related to an increase of the tissue hydration.
Baby
André Rolim
AR
Department of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, 580 Prof. Lineu Prestes Av., bloco 13, Conjunto das Químicas, Cidade Universitária, 05508-900 São Paulo, SP, Brazil. andrerb@usp.br
Lacerda
Aurea Cristina Lemos
AC
Velasco
Maria Valéria Robles
MV
Lopes
Patrícia Santos
PS
Kawano
Yoshio
Y
Kaneko
Telma Mary
TM
D000818:Animals; D017837:Bothrops; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D004817:Epidermis / Q000737:chemistry* / Q000187:drug effects; D008566:Membranes / Q000737:chemistry* / Q000187:drug effects; D008954:Models, Biological; D012996:Solutions / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology; D014867:Water / Q000737:chemistry
D002593:Cetrimonium Compounds; D012996:Solutions; D013501:Surface-Active Agents; D014867:Water; D000077286:Cetrimonium