[Effects of quercetin on morphology and VEGF secretion of leukemia cells NB4 in vitro].
OBJECTIVE
To investigate the effects of quercetin on cell morphology and VEGF expression of acute myeloblastic leukemia cells NB4 in vitro.


METHODS
The cytomorphology of NB4 cells was assessed by Wright-stain, apoptosis rate by apoptotic marker Annexin V, and VEGF secretion level by ELISA.


RESULTS
Typical apoptosis was found in NB4 cells after treatment with quercetin. Apoptotic marker Annexin V analysis showed that the apoptotic rate of NB4 cells was increased after treatment with quercetin. The secretion of VEGF of NB4 cells was significantly decreased after treatment with quercetin.


CONCLUSIONS
Quercetin can induce apoptosis and inhibit secretion of VEGF in NB4 leukemia cells.
Zhong
Lu
L
Department of Hematotogy, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Chen
Fang-yuan
FY
Wang
Hai-rong
HR
Ten
Ye
Y
Wang
Chen
C
Ouyang
Ren-rong
RR
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology; D011794:Quercetin / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D042461:Vascular Endothelial Growth Factor A; D011794:Quercetin