title: Small-angle X-ray scattering reveals an extended organization for the autoinhibitory resting state of the p47(phox) modular protein.
abstract: In response to microbial infection, neutrophiles promote the assembly of the NADPH oxidase complex in order to produce superoxide anions. This reaction is activated by the association of cytosolic factors, p47(phox), p67(phox), p40(phox), and a small G protein Rac with the membranous heterodimeric flavocytochrome b(558), composed of gp91(phox) and p22(phox). In the activation process, p47(phox) plays a central role as the target of phosphorylations and as a scaffolding protein conducting the translocation and assembly of cytosolic factors onto the membranous components. The PX and tandem SH3s of p47(phox) have been highlighted as being key determinants for the interaction with membrane lipids and the p22(phox) component, respectively. In the resting state, the two corresponding interfaces are thought to be masked allowing its cytoplasmic localization. However, the resting state modular organization of p47(phox) and its autoinhibition mode are still not fully understood despite available structural information on separate modules. More precisely, it raises the question of the mutual arrangement of the PX domain and the tandem SH3 domains in the resting state. To address this question, we have engaged a study of the entire p47(phox) molecule in solution using small-angle X-ray scattering. Despite internal autoinhibitory interactions, p47(phox) adopts an extended conformation. First insights about the domain arrangement in whole p47(phox) can be derived. Our data allow to discard the usual representation of a globular and compact autoinhibited resting state.
authors.affiliation: IBBMC, CNRS UMR8619 Université Paris Sud, Bât. 430, 91405 Orsay Cedex, France. dominique.durand@ibbmc.u-psud.fr
authors.forename: Dominique
authors.initials: D
authors.lastname: Durand
authors.forename: Dominique
authors.initials: D
authors.lastname: Cannella
authors.forename: Virginie
authors.initials: V
authors.lastname: Dubosclard
authors.forename: Eva
authors.initials: E
authors.lastname: Pebay-Peyroula
authors.forename: Patrice
authors.initials: P
authors.lastname: Vachette
authors.forename: Franck
authors.initials: F
authors.lastname: Fieschi
mesh_terms: D000595:Amino Acid Sequence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019255:NADPH Oxidases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012542:Scattering, Radiation; D017386:Sequence Homology, Amino Acid; D014965:X-Rays
chemical_list: D011994:Recombinant Proteins; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1