title: Global analysis of protein palmitoylation in yeast.
abstract: Protein palmitoylation is a reversible lipid modification that regulates membrane tethering for key proteins in cell signaling, cancer, neuronal transmission, and membrane trafficking. Palmitoylation has proven to be a difficult study: Specifying consensuses for predicting palmitoylation remain unavailable, and first-example palmitoylation enzymes--i.e., protein acyltransferases (PATs)--were identified only recently. Here, we use a new proteomic methodology that purifies and identifies palmitoylated proteins to characterize the palmitoyl proteome of the yeast Saccharomyces cerevisiae. Thirty-five new palmitoyl proteins are identified, including many SNARE proteins and amino acid permeases as well as many other participants in cellular signaling and membrane trafficking. Analysis of mutant yeast strains defective for members of the DHHC protein family, a putative PAT family, allows a matching of substrate palmitoyl proteins to modifying PATs and reveals the DHHC family to be a family of diverse PAT specificities responsible for most of the palmitoylation within the cell.
authors.affiliation: Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Amy F
authors.initials: AF
authors.lastname: Roth
authors.forename: Junmei
authors.initials: J
authors.lastname: Wan
authors.forename: Aaron O
authors.initials: AO
authors.lastname: Bailey
authors.forename: Beimeng
authors.initials: B
authors.lastname: Sun
authors.forename: Jason A
authors.initials: JA
authors.lastname: Kuchar
authors.forename: William N
authors.initials: WN
authors.lastname: Green
authors.forename: Brett S
authors.initials: BS
authors.lastname: Phinney
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Davis
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D000217:Acyltransferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D019308:Palmitic Acid / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology; D020543:Proteome / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D019308:Palmitic Acid; D000217:Acyltransferases; C511958:Pfa4 protein, S cerevisiae; D000123:Acetyltransferases; C062782:protein acyltransferase