The uptake of glycerol, alpha-aminoisobutyric acid and 2-deoxy-D-glucose by spermatozoa of a line of chickens selected for fertility of frozen-thawed semen and a control line.
The uptake of glycerol, alpha-aminoisobutyric acid and 2-deoxy-D-glucose by fowl spermatozoa was each measured in two trials using seventh generation males of a line selected for duration of fertility of frozen-thawed semen and those of a randomly selected control line. The selected line had significantly (P< 0.01) higher fertility of frozen-thawed and fresh semen than the control line. The association between glycerol level in spermatozoa before freezing and the fertility of frozen-thawed semen was examined. Significantly greater amounts (CPM/10(9) cells) of glycerol, alpha-aminoisobutyric acid and 2-deoxy-D-glucose were taken up by the spermatozoa of the selected line than those of the control line at 15, 30 and 60 min of incubation. Rank correlations between the fertility of frozen-thawed semen and glycerol levels were generally positive but low in magnitudes.
Ansah
G A
GA
Department of Animal Science Macdonald Campus of McGill University 21,111 Lakeshore Road Ste-Anne de Bellevue Québec, Canada H9X 1C0.
Buckland
R B
RB