Molecular characterization and expression analysis of the porcine caveolin-3 gene.
Caveolin-3 is the muscle-specific form of the caveolin protein family and plays an important role in modulating both the morphological appearance and function of caveolae. In this study, we cloned and characterized caveolin-3 from porcine muscle. The promoter of porcine caveolin-3 contained three consensus E box elements and one RORalpha2 monomeric binding motif. The deduced amino acid sequence of porcine caveolin-3 contains a WW domain. This gene was mapped to SSC13 q23-q24 by the SCHP and the IMpRH panel. RT-PCR analyses showed that caveolin-3 was expressed specifically in skeletal muscle and heart. And we provide the first evidence that caveolin-3 has a certain regulated expression pattern during the prenatal period of the porcine skeletal muscle development. This result suggests that the caveolin-3 gene might be a candidate gene of meat production trait and provides some information for establishing of an animal model using pig to study human caveolinopathies.
Zhu
Zhengmao
Z
Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China.
Li
Yong
Y
Mo
Delin
D
Li
Kui
K
Zhao
Shuhong
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051244:Caveolin 3 / Q000235:genetics*; D003001:Cloning, Molecular; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D016415:Sequence Alignment; D013552:Swine; D014018:Tissue Distribution
D051244:Caveolin 3