title: [Diagnostic performance of specific IgM detection and genomic amplification in rubella].
abstract: Nowadays, most exanthematic diseases for which a vaccine is available affect young adults. A large percentage of these cases prove to be rubella. The aim of this study is to assess the performance of specific IgM and RT-PCR for the diagnosis of rubella infection. Fifty-nine patients with clinically suspected measles or rubella, and with available serum, whole blood, urine and pharyngeal exudate specimens were studied. RT-PCR in pharyngeal exudate was found to be the most effective marker at the start of the disease (mean, 2.5 days). IgM detection yielded a larger percentage of positive results (76.2%), but at a later time (3.7 days).
authors.affiliation: Servicio de Microbiología Diagnóstica, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. de Majadahonda-Pozuelo s/n, 28220 Majadahonda, Madrid, Spain. mmosquera@isciii.es
authors.forename: María del Mar
authors.initials: Mdel M
authors.lastname: Mosquera
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Sanz
authors.forename: Juan Emilio
authors.initials: JE
authors.lastname: Echevarría
authors.forename: Nieves
authors.initials: N
authors.lastname: Herranz
authors.forename: Marisa
authors.initials: M
authors.lastname: Fernández
authors.forename: Fernando
authors.initials: F
authors.lastname: de Ory
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010614:Pharynx / Q000821:virology; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012409:Rubella / Q000097:blood / Q000175:diagnosis* / Q000652:urine / Q000821:virology; D012412:Rubella virus / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M; D012367:RNA, Viral