title: The expression of the homologue of the Caenorhabditis elegans lin-45 raf is regulated in the motile stages of the plant parasitic nematode Meloidogyne artiellia.
abstract: The Ras-MAPK signal transduction pathway controls multiple developmental events and is involved in the processing of olfactory information in the free living nematode Caenorhabditis elegans. We have studied the Ras-MAPK pathway in the plant parasitic nematode Meloidogyne artiellia. The genes Mt-let-60, Mt-lin-45, Mt-mek-2 and Mt-mpk-1 have been isolated and sequenced. Each of them shows a high level of sequence similarity to its presumed ortholog in C. elegans and key functional domains are structurally conserved. Furthermore, we show that the M. artiellia recombinant MEK-2 protein can phosphorylate and activate the M. artiellia recombinant MPK-1 and the recombinant MEK-2 itself can be phosphorylated and activated by immunoprecipitated mammalian Raf. Surprisingly, the Mt-lin-45 message is not detectable in freshly emerged juveniles or in male specimens, suggesting that it may be quickly degraded in these life stages.
authors.affiliation: Dipartimento di Biochimica e Biologia Molecolare, Via Orabona 4, 70126 Bari, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Cortese
authors.forename: Mauro
authors.initials: M
authors.lastname: Di Vito
authors.forename: Carla
authors.initials: C
authors.lastname: De Giorgi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D029863:Cicer / Q000469:parasitology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D048370:MAP Kinase Kinase 2 / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009833:Olfactory Pathways; D010766:Phosphorylation; D016415:Sequence Alignment; D015398:Signal Transduction; D014431:Tylenchoidea / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D048490:raf Kinases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; C454817:lin-45 protein, C elegans; D048490:raf Kinases; D020928:Mitogen-Activated Protein Kinases; D048370:MAP Kinase Kinase 2; D018631:ras Proteins