title: Spectroscopic studies of stratum corneum model membrane from Bothrops jararaca treated with cationic surfactant.
abstract: This research employed FT-Raman and PAS-FTIR spectroscopic techniques to evaluate the interaction of cetyl trimethyl ammonium chloride (CTAC), a cationic surfactant, on the stratum corneum (SC) of shed snake skins from Bothrops jararaca, used as model membranes. Surfactant aqueous solutions (50.0 and 0.78 gl(-1)) with neutral pH were applied on the samples with intervals of 4, 8 (whole SC) and 12h (SC tape-stripped). Samples presented modifications of the topography for all conditions of the assays and the monomers of the surfactant, instead of the micelles, seemed to interact with the keratin. The SC model membranes treated with CTAC have had an augment of water content (except for whole SC treated for 8h) indicated by the expansion of the band 3600-3300 cm(-1), mainly for the tape-stripped samples after 12h treatment. Concentration appeared to be an important factor related to an increase of the tissue hydration.
authors.affiliation: Department of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, 580 Prof. Lineu Prestes Av., bloco 13, Conjunto das Químicas, Cidade Universitária, 05508-900 São Paulo, SP, Brazil. andrerb@usp.br
authors.forename: André Rolim
authors.initials: AR
authors.lastname: Baby
authors.forename: Aurea Cristina Lemos
authors.initials: AC
authors.lastname: Lacerda
authors.forename: Maria Valéria Robles
authors.initials: MV
authors.lastname: Velasco
authors.forename: Patrícia Santos
authors.initials: PS
authors.lastname: Lopes
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kawano
authors.forename: Telma Mary
authors.initials: TM
authors.lastname: Kaneko
mesh_terms: D000818:Animals; D017837:Bothrops; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D004817:Epidermis / Q000737:chemistry* / Q000187:drug effects; D008566:Membranes / Q000737:chemistry* / Q000187:drug effects; D008954:Models, Biological; D012996:Solutions / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology; D014867:Water / Q000737:chemistry
chemical_list: D002593:Cetrimonium Compounds; D012996:Solutions; D013501:Surface-Active Agents; D014867:Water; D000077286:Cetrimonium