title: Multivariate optimisation of the microwave-assisted extraction of oleuropein and related biophenols from olive leaves.
abstract: Microwave assistance is proposed for the first time in order to accelerate the extraction of biophenols from olive leaves. Under optimal working conditions, obtained using a multivariate methodology, complete extraction of the target analytes was achieved in 8 min. The extracts required no clean-up nor concentration prior to injection into a chromatograph-photodiode array detector assembly for individual separation-quantification. The optimal extractant (an 80:20 ethanol-water mixture) was also used in the development of a stirring-based extraction method which required around 24 h for complete extraction of the target compounds. These mixtures can be used as replacements for toxic extractants, with a view to exploiting olive leaves in order to obtain biophenols for human use.
authors.affiliation: Analytical Chemistry Department, Annex Marie Curie Building, Rabanales Campus, University of Córdoba, 14071 Córdoba, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Japón-Luján
authors.forename: J M
authors.initials: JM
authors.lastname: Luque-Rodríguez
authors.forename: M D
authors.initials: MD
authors.lastname: Luque de Castro
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D057888:Iridoid Glucosides; D039823:Iridoids; D007700:Kinetics; D008872:Microwaves; D015394:Molecular Structure; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011714:Pyrans / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D057888:Iridoid Glucosides; D039823:Iridoids; D010636:Phenols; D010936:Plant Extracts; D011714:Pyrans; C002769:oleuropein