Standardisation of Gymnema sylvestre R.Br. by high-performance thin-layer chromatography: an improved method.
An improved high-performance thin-layer chromatographic (HPTLC) method for the standardisation of Gymnema sylvestre is reported. The method involves the initial hydrolysis of gymnemic acids, the active ingredients, to a common aglycone followed by the quantitative estimation of gymnemagenin. The present method rectifies an error found in an HPTLC method reported recently.
Raju
Valivarthi S R
VS
Laila Impex Research Centre, Unit I, Phase III, Jawahar Autonagar, Vijayawada -520 007, India.
Kannababu
S
S
Subbaraju
Gottumukkala V
GV
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D031104:Gymnema sylvestre / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry
D012503:Saponins; D014315:Triterpenes; C007898:gymnemic acid