title: A rat model of bone cancer pain induced by intra-tibia inoculation of Walker 256 mammary gland carcinoma cells.
abstract: This study described a modified rat model of bone cancer pain. Syngeneic Walker 256 mammary gland carcinoma cells were injected into the tibia medullary cavity via intercondylar eminence. Series of tests were carried out including bone radiology, bone histology, ambulatory pain, thermal hyperalgesia, mechanical allodynia, weight bearing ability, and electrophysiological recording from primary afferent fibers. The rats inoculated with carcinoma cells showed significant ambulatory pain, mechanical allodynia, and reduction in weight bearing, as well as increased incidence of spontaneous activity in Abeta fibers in affected limb, whereas PBS (vehicle) or heat-killed cells (sham) injected rats showed no significant difference in comparison to normal rats. The pain hypersensitive behaviors were aggravated with time and destruction of bone. Interestingly, mechanical allodynia was also observed in the contralateral limb, indicating the involvement of 'mirror image' pain in bone cancer pain. In summary, the present study provided a useful and easily established rat model of bone cancer pain which will contribute to further study of the mechanisms underlying cancer pain.
authors.affiliation: Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Fudan University, China.
authors.forename: Qi-Liang
authors.initials: QL
authors.lastname: Mao-Ying
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Dong
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Yu
authors.forename: Min-Fen
authors.initials: MF
authors.lastname: Yan
authors.forename: Yu-Qiu
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Gen-Cheng
authors.initials: GC
authors.lastname: Wu
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Wang
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001859:Bone Neoplasms / Q000150:complications*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004558:Electric Stimulation; D005260:Female; D006614:Hindlimb / Q000503:physiopathology; D006930:Hyperalgesia / Q000209:etiology / Q000503:physiopathology; D015674:Mammary Neoplasms, Animal / Q000473:pathology; D009368:Neoplasm Transplantation; D009412:Nerve Fibers / Q000502:physiology; D009437:Neuralgia / Q000209:etiology / Q000503:physiopathology; D010146:Pain / Q000209:etiology / Q000503:physiopathology*; D010147:Pain Measurement / Q000379:methods; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology; D013979:Tibial Nerve / Q000503:physiopathology; D013997:Time Factors; D016474:Weight-Bearing / Q000502:physiology