Effect of high frequency transcutaneous electrical nerve stimulation on viability of random skin flap in rats.
OBJECTIVE
To determine the effect of high frequency Transcutaneous Electrical Nerve Stimulation (TENS) on viability of random skin flap in rats.


METHODS
The sample of this study was 75 Wistar rats. The skin flap measured 10 x 4 cm and a plastic barrier was interposed between the flap and donor site. After the operative procedure, animals of all groups were maintained anesthetized one more hour with electrodes positioned in the base of the flap and submitted to treatment according of their respective group. This procedure was repeated on the two subsequent days. G1: sham stimulation (control), G2: TENS (f = 80 Hz and I = 5 mA), G3: TENS (f = 80 Hz and I = 10 mA), G4: TENS (f = 80 Hz and I = 15 mA), G5: TENS (f = 80 Hz and I = 20 mA).


RESULTS
The average percentage of necrotic area was 43,11, 34,65, 49,44, 23,52, 45,10 in groups 1, 2, 3, 4 and 5 respectively.


CONCLUSIONS
The amplitude of 15 mA presented a lower necrotic area than control group and Transcutaneous Electrical Nerve Stimulation was efficient in increasing the random skin flap viability.
Liebano
Richard Eloin
RE
EPM, UNIFESP, Brazil. liebano@gmail.com
Abla
Luiz Eduardo Felipe
LE
Ferreira
Lydia Masako
LM
D000704:Analysis of Variance; D000818:Animals; D006085:Graft Survival / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar; D013524:Surgical Flaps / Q000098:blood supply / Q000473:pathology; D004561:Transcutaneous Electric Nerve Stimulation