DNA binding by the Methanothermobacter thermautotrophicus Cdc6 protein is inhibited by the minichromosome maintenance helicase.
The Cdc6 proteins from the archaeon Methanothermobacter thermautotrophicus were previously shown to bind double-stranded DNA. It is shown here that the proteins also bind single-stranded DNA. Using minichromosome maintenance (MCM) helicase mutant proteins unable to bind DNA, it was found that the interaction of MCM with Cdc6 inhibits the DNA binding activity of Cdc6.
Kasiviswanathan
Rajesh
R
University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, 9600 Gudelsky Drive, Rockville, MD 20850, USA.
Shin
Jae-Ho
JH
Kelman
Zvi
Z
D019843:Archaeal Proteins / Q000378:metabolism / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D004265:DNA Helicases / Q000502:physiology*; D004261:DNA Replication; D019641:DNA, Archaeal / Q000096:biosynthesis / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D017013:Methanobacteriaceae / Q000201:enzymology / Q000235:genetics*; D011485:Protein Binding
D019843:Archaeal Proteins; D018797:Cell Cycle Proteins; D019641:DNA, Archaeal; D004268:DNA-Binding Proteins; D004265:DNA Helicases