Genome-wide analysis of Polycomb targets in Drosophila melanogaster.
Polycomb group (PcG) complexes are multiprotein assemblages that bind to chromatin and establish chromatin states leading to epigenetic silencing. PcG proteins regulate homeotic genes in flies and vertebrates, but little is known about other PcG targets and the role of the PcG in development, differentiation and disease. Here, we determined the distribution of the PcG proteins PC, E(Z) and PSC and of trimethylation of histone H3 Lys27 (me3K27) in the D. melanogaster genome. At more than 200 PcG target genes, binding sites for the three PcG proteins colocalize to presumptive Polycomb response elements (PREs). In contrast, H3 me3K27 forms broad domains including the entire transcription unit and regulatory regions. PcG targets are highly enriched in genes encoding transcription factors, but they also include genes coding for receptors, signaling proteins, morphogens and regulators representing all major developmental pathways.
Schwartz
Yuri B
YB
Rutgers University, Department of Molecular Biology and Biochemistry, 604 Allison Road, Piscataway, New Jersey 08854, USA.
Kahn
Tatyana G
TG
Nix
David A
DA
Li
Xiao-Yong
XY
Bourgon
Richard
R
Biggin
Mark
M
Pirrotta
Vincenzo
V
D000818:Animals; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D019175:DNA Methylation; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D016678:Genome; D063150:Polycomb Repressive Complex 1; D020133:Reverse Transcriptase Polymerase Chain Reaction
D029721:Drosophila Proteins; C066770:Pc protein, Drosophila; D063150:Polycomb Repressive Complex 1