In vitro characterization of peptide-modified p(AAm-co-EG/AAc) IPN-coated titanium implants.
Interpenetrating polymer networks (IPNs) of poly(acrylamide-co-ethylene glycol/acrylic acid) [p(AAm-co-EG/AAc)] functionalized with an -Arg-Gly-Asp- containing peptide derived from rat bone sialoprotein [bsp-RGD(15)] were grafted to titanium implants in an effort to modulate osteoblast behavior in vitro. Surface characterization data were consistent with the presence of an IPN, and ligand density measurements established that the range of peptide density on the modified implants spanned three orders of magnitude (0.01-20 pmol/cm2). In vitro biological characterization of the modified implants employing the primary rat calvarial osteoblast (RCO) model resulted in the identification of a critical ligand density (0.01<Gammacrit<0.1 pmol/cm2) for maximal support of the osteoblast phenotype. After 14 and 21 days, mineralization was greater on the 0.1 and 10 pmol/cm2 bsp-RGD(15) modified implants compared to the base titanium and other control surfaces. The observed effects were attributed to specific interactions with bsp-RGD(15) and support the concept that peptide-modified implants can enhance the kinetics of differentiation of the cells they contact. These results suggest that in vivo biological performance evaluation of these biomimetic implant surfaces is merited.
Barber
Thomas A
TA
Department of Bioengineering, University of California at Berkeley, Berkeley, California 94720, USA.
Gamble
Lara J
LJ
Castner
David G
DG
Healy
Kevin E
KE
D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D020007:Implants, Experimental; D058574:Integrin-Binding Sialoprotein; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D012795:Sialoglycoproteins / Q000378:metabolism; D046590:Spectroscopy, Electron Energy-Loss; D014025:Titanium
D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D010455:Peptides; D012795:Sialoglycoproteins; C513408:bsp-RGD(15) peptide; C115156:p(AAM-co-EG-AA)IPN; D011092:Polyethylene Glycols; D014025:Titanium