Characterisation of the oral adjuvant effect of lemnan, a pectic polysaccharide of Lemna minor L.
Lemnan LM, apiogalacturonanic pectin of duckweed Lemna minor L. was tested for adjuvant properties following oral administration with protein antigen. Male Swiss mice were orally immunized thrice with weekly intervals with free OVA or OVA with lemnan (LM). Lemnan LM was shown to increase delayed type hypersensitivity (DTH) and serum anti OVA IgG responses. LM was established to increase levels of both serum IgG1 and IgG2a subclasses, intestinal IgA and failed to elevate levels of serum IgE. Lemnan was found to increase the adhesion of macrophages and to enhance the generation of oxygen radicals by macrophages in response to phorbol 12-myristate 13-acetate. Serum OVA levels were four-fold higher in mice immunized with the mixture of OVA and LM in comparison with those in mice immunized with OVA only. Thus, substantial systemic and local mucosal immune responses were attained by oral immunization with the mixture of OVA and lemnan. Lemnan appeared to elicit adjuvant activity via induction of both Th1- and Th2-type responses. The immunopotentiating effect of lemnan may result from enhanced antigen ingestion and stimulation of macrophage activity.
Popov
Sergey V
SV
Institute of Physiology, Komi Sci. Ctr., The Urals Branch of the Russian Academy of Sciences, 50 Pervomaiskaya Str., 167982 Syktyvkar, Russia. popov@physiol.komisc.ru
Golovchenko
Victoria V
VV
Ovodova
Raisa G
RG
Smirnov
Vasily V
VV
Khramova
Daria S
DS
Popova
Galina Yu
GY
Ovodov
Yury S
YS
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D029064:Araceae / Q000737:chemistry*; D005767:Gastrointestinal Diseases / Q000139:chemically induced; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D010368:Pectins / Q000008:administration & dosage* / Q000009:adverse effects / Q000276:immunology*; D010586:Phagocytes
D000276:Adjuvants, Immunologic; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C423783:lemnan; D010368:Pectins; D010047:Ovalbumin