[Relationship between genetic polymorphisms of Interferon-gamma gene intron 1 +874 site and susceptibility of hepatitis B virus infection].
OBJECTIVE
To explore the possible relationship between the polymorphisms of interferon-gamma (IFN-gamma) gene intron 1 at position +874 and the susceptibility of hepatitis B virus infection.


METHODS
IFN-gamma gene single nucleotide polymorphism (intron 1 at position +874) were detected in 531 subjects including 231 chronic carrier of hepatitis B virus (group CC), 165 individuals who spontaneously recovered from HBV infection (group SR) and 135 healthy controls, by polymerase chain reaction with sequence specific primers.


RESULTS
Significant difference of IFN-gamma +874 AA genotype frequency was found between group CC (TT, TA and AA frequencies were 9.1%, 12.1% and 78.8%, respectively) and control group (TT, TA and AA frequencies were 12.6%, 23.0% and 64.4%, respectively) (chi(2) = 9.60, P = 0.008), but no significant difference found between group SR (TT, TA and AA frequencies were 13.9%, 23.7% and 62.4%, respectively) and control group (chi(2) = 0.16, P = 0.92).


CONCLUSIONS
There was association between IFN-gamma gene polymorphism (intron 1 at position +874) and chronic HBV infection. This study suggested the possibility that IFN-gamma gene polymorphism (intron 1 at position +874) might be important in determining an individual's susceptibility in the development of chronic HBV infection.
Zhang
Ping-an
PA
Department of Laboratory Science, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Wu
Jian-min
JM
Li
Yan
Y
D002353:Carrier State; D016022:Case-Control Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006515:Hepatitis B virus; D019694:Hepatitis B, Chronic / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D007438:Introns; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D007371:Interferon-gamma