title: Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus.
abstract: To validate the use of serology in substantiating freedom from infection after foot-and-mouth disease (FMD) outbreaks have been controlled by measures that include vaccination, 3551 sera were tested with six assays that detect antibodies to the non-structural proteins of FMD virus. The sera came from naïve, vaccinated, infected and vaccinated-and-infected animals; two-thirds from cattle, the remainder from sheep and pigs. The assays were covariant for sensitivity, but not necessarily for specificity. A commercial kit from Cedi-diagnostics and an in-house assay from IZS-Brescia were comparable to the NCPanaftosa-screening index method described in the Diagnostic Manual of the World Animal Health Organisation. Using these three tests the specificity and sensitivity for the detection of carriers in vaccinated cattle approaches or exceeds 99% and 90%, respectively.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), Via Bianchi 7/9, Brescia, Italy. ebrocchi@bs.izs.it
authors.forename: E
authors.initials: E
authors.lastname: Brocchi
authors.forename: I E
authors.initials: IE
authors.lastname: Bergmann
authors.forename: A
authors.initials: A
authors.lastname: Dekker
authors.forename: D J
authors.initials: DJ
authors.lastname: Paton
authors.forename: D J
authors.initials: DJ
authors.lastname: Sammin
authors.forename: M
authors.initials: M
authors.lastname: Greiner
authors.forename: S
authors.initials: S
authors.lastname: Grazioli
authors.forename: F
authors.initials: F
authors.lastname: De Simone
authors.forename: H
authors.initials: H
authors.lastname: Yadin
authors.forename: B
authors.initials: B
authors.lastname: Haas
authors.forename: N
authors.initials: N
authors.lastname: Bulut
authors.forename: V
authors.initials: V
authors.lastname: Malirat
authors.forename: E
authors.initials: E
authors.lastname: Neitzert
authors.forename: N
authors.initials: N
authors.lastname: Goris
authors.forename: S
authors.initials: S
authors.lastname: Parida
authors.forename: K
authors.initials: K
authors.lastname: Sørensen
authors.forename: K
authors.initials: K
authors.lastname: De Clercq
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D002353:Carrier State / Q000276:immunology; D002417:Cattle; D016208:Databases, Factual; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D015203:Reproducibility of Results; D012756:Sheep; D013552:Swine; D014611:Vaccination; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014764:Viral Proteins