Nonneutralizing antibodies are able to inhibit human immunodeficiency virus type 1 replication in macrophages and immature dendritic cells.
Only five monoclonal antibodies (MAbs) neutralizing a broad range of primary isolates (PI) have been identified up to now. We have found that some MAbs with no neutralizing activities according to the "conventional" neutralization assay, involving phytohemagglutinin-stimulated peripheral blood mononuclear cells as targets, efficiently inhibit the replication of human immunodeficiency virus type 1 (HIV-1) PI in macrophages and immature dendritic cells (iDC). The mechanism of inhibition is distinct from the neutralization of infectivity occurring via Fab fragments and involves the interaction of the F portion with the FcgammaRs present on macrophages and iDC. We propose that, if such nonneutralizing inhibitory antibodies limit mucosal HIV transmission, they should be induced by vaccination.
Holl
Vincent
V
EA 3770, ULP, Institut de Virologie, 3 rue Koeberlé, F-67000 Strasbourg, France.
Peressin
Maryse
M
Decoville
Thomas
T
Schmidt
Sylvie
S
Zolla-Pazner
Susan
S
Aubertin
Anne-Marie
AM
Moog
Christiane
C
D002460:Cell Line; D003713:Dendritic Cells / Q000821:virology*; D015483:HIV Antibodies / Q000494:pharmacology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D008264:Macrophages / Q000821:virology*; D009500:Neutralization Tests; D017452:Receptors, IgG; D014779:Virus Replication / Q000187:drug effects / Q000276:immunology*
D015483:HIV Antibodies; D007140:Immunoglobulin Fab Fragments; D017452:Receptors, IgG