title: Plasmodium falciparum genotype population dynamics in asymptomatic children from Senegal.
abstract: In areas where malaria is endemic, infected individuals generally harbor a mixture of genetically distinct Plasmodium falciparum parasite populations. For the first time, we studied temporal variations of blood parasite densities and circulating genotypes in asymptomatic Senegalese children, at time intervals as short as 4-12 h. Twenty-one Senegalese children, presenting with an asymptomatic P. falciparum infection, were sampled eight times within three days. Parasite density was assessed by thick blood smears, and all infecting genotypes were quantified by the fragment-analysis method. Parasite densities showed dramatic fluctuations up to a 1 to 1,000 ratio, with at least one peak of parasite density. Polyclonal infections were detected in all children, with a multiplicity of infection of 5.2-6.8 genotypes per child. A single sample never reflected the full complexity of the parasite populations hosted by a given individual. Genotypes with different behaviors were detected in all children, some genotypes undergoing major fluctuations, while others were highly stable during the follow-up. A single peripheral blood sampling does not reflect the total parasite load. Repeated sampling is needed to have a more detailed scheme of parasite population dynamics and a better knowledge of the true complexity of an infection.
authors.affiliation: UR 010, Mother and Child Health in the Tropics, Institut de Recherche pour le Développement (IRD), Faculté de Pharmacie, 4, Avenue de l'Observatoire, Paris, France.
authors.forename: Sayeh
authors.initials: S
authors.lastname: Jafari-Guemouri
authors.forename: Christian
authors.initials: C
authors.lastname: Boudin
authors.forename: Nadine
authors.initials: N
authors.lastname: Fievet
authors.forename: Pape
authors.initials: P
authors.lastname: Ndiaye
authors.forename: Philippe
authors.initials: P
authors.lastname: Deloron
mesh_terms: D000293:Adolescent; D000818:Animals; D001769:Blood / Q000469:parasitology; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000235:genetics; D019353:Endemic Diseases; D005838:Genotype; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology* / Q000469:parasitology*; D018512:Parasitemia; D010963:Plasmodium falciparum / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D012675:Senegal; D013997:Time Factors
chemical_list: D016054:DNA, Protozoan