title: Oviductal and uterine influence on the development of Day-2 equine embryos in vivo and in vitro.
abstract: The objective of this experiment was to contrast the influence of the oviductal and uterine environments on development of Day-2 embryos. Embryos were transferred to oviducts or uteri of synchronous recipient mares, or were incubated in oviductal co-culture, in uterine co-culture or in defined culture medium. Significantly more (P < 0.02) embryos transferred to the oviduct versus the uterus survived until Day 11 after ovulation (5 7 vs 0 7 , respectively). Significantly more (P < 0.001) embryos developed to expanded and hatched blastocysts in uterine co-culture than in culture medium (6 7 vs 0 7 , respectively). The rate of embryo development to expanded blastocysts was not significantly different (P > 0.1) in oviductal co-culture versus uterine co-culture (3 7 vs 6 7 , respectively), or in oviductal co-culture versus culture in medium (3 7 vs 0 7 , respectively). Three of 7 and 6 of 7 embryos developed to hatched blastocysts greater than 2000 mum in diameter during oviductal and uterine co-culture, respectively, while 0 of 7 embryos cultured in medium expanded to greater than 500 mum in diameter. Proportions of embryos that developed for at least 9 days.
authors.affiliation: Northwest Equine Reproduction Laboratory Department of Animal and Veterinary Science University of Idaho, Moscow, ID 83844-2201 USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Weber
authors.forename: G L
authors.initials: GL
authors.lastname: Woods
authors.forename: D A
authors.initials: DA
authors.lastname: Freeman
authors.forename: D K
authors.initials: DK
authors.lastname: Vanderwall