title: Polyclonal antibodies specific for VP1 and VP3 capsid proteins of Taura syndrome virus (TSV) produced via gene cloning and expression.
abstract: Capsid protein genes VP1 and VP3 of Taura syndrome virus (TSV) were cloned into pGEX-6P-1 expression vector and transformed into Escherichia coli BL21. After induction, recombinant VP1 (rVP1) and recombinant VP3 (rVP3) were produced, purified by SDS-PAGE and used for immunization of Swiss mice for antisera production. Anti-rVP1 and anti-rVP3 antisera showed specific immunoreactivities to rVP1 and rVP3 proteins, respectively, by Western blot assay and also yielded good results for detection of TSV in various shrimp tissues by immunohistochemistry. This is the first step towards our target of preparing monoclonal antibodies specific to rVP1 and rVP3 for use in simple immuno-diagnostic test kits for TSV detection and identification.
authors.affiliation: Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, Thailand.
authors.forename: Parin
authors.initials: P
authors.lastname: Chaivisuthangkura
authors.forename: Thanawan
authors.initials: T
authors.lastname: Tejangkura
authors.forename: Sombat
authors.initials: S
authors.lastname: Rukpratanporn
authors.forename: Siwaporn
authors.initials: S
authors.lastname: Longyant
authors.forename: Weerawan
authors.initials: W
authors.lastname: Sithigorngul
authors.forename: Paisarn
authors.initials: P
authors.lastname: Sithigorngul
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D015153:Blotting, Western / Q000379:methods; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry / Q000662:veterinary; D051379:Mice; D033561:Penaeidae / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D017931:DNA Primers; D007106:Immune Sera; D011994:Recombinant Proteins