title: Transferable bread wheat EST-SSRs can be useful for phylogenetic studies among the Triticeae species.
abstract: The genetic similarity between 150 accessions, representing 14 diploidand polyploid species of the Triticeae tribe, was investigated following the UPGMA clustering method. Seventy-three common wheat EST-derived SSR markers (EST-SSRs) that were demonstrated to be transferable across several wheat-related species were used. When diploid species only are concerned, all the accessions bearing the same genome were clustered together without ambiguity while the separation between the different sub-species of tetraploid as well as hexaploid wheats was less clear. Dendrograms reconstructed based on data of 16 EST-SSRs mapped on the A genome confirmed that Triticum aestivum and Triticum durum had closer relationships with Triticum urartu than with Triticum monococcum and Triticum boeoticum, supporting the evidence that T. urartu is the A-genome ancestor of polyploid wheats. Similarly, another tree reconstructed based on data of ten EST-SSRs mapped on the B genome showed that Aegilops speltoides had the closest relationship with T. aestivum and T. durum, suggesting that it was the main contributor of the B genome of polyploid wheats. All these results were expected and demonstrate thus that EST-SSR markers are powerful enough for phylogenetic analysis among the Triticeae tribe.
authors.affiliation: UMR INRA-UBP Amélioration et Santé des Plantes, Domaine de Crouël, 234 Avenue du Brézet, 63100, Clermont-Ferrand, France.
authors.forename: L Y
authors.initials: LY
authors.lastname: Zhang
authors.forename: C
authors.initials: C
authors.lastname: Ravel
authors.forename: M
authors.initials: M
authors.lastname: Bernard
authors.forename: F
authors.initials: F
authors.lastname: Balfourier
authors.forename: P
authors.initials: P
authors.lastname: Leroy
authors.forename: C
authors.initials: C
authors.lastname: Feuillet
authors.forename: P
authors.initials: P
authors.lastname: Sourdille
mesh_terms: D016000:Cluster Analysis; D020224:Expressed Sequence Tags; D018745:Genome, Plant; D018895:Microsatellite Repeats; D010802:Phylogeny; D011003:Ploidies; D006109:Poaceae / Q000145:classification* / Q000235:genetics; D014908:Triticum / Q000145:classification / Q000235:genetics