title: Mrc1 and Srs2 are major actors in the regulation of spontaneous crossover.
abstract: In vegetative cells, most recombination intermediates are metabolized without an association with a crossover (CO). The avoidance of COs allows for repair and prevents genomic rearrangements, potentially deleterious if the sequences involved are at ectopic locations. We have designed a system that permits to screen spontaneous intragenic recombination events in Saccharomyces cerevisiae and to investigate the CO outcome in different genetic contexts. We have analyzed the CO outcome in the absence of the Srs2 and Sgs1 helicases, DNA damage checkpoint proteins as well as in a mutant proliferating cell nuclear antigen (PCNA) and found that they all contribute to genome stability. Remarkably high effects on COs are mediated by srs2Delta, mrc1Delta and a pol30-RR mutation in PCNA. Our results support the view that Mrc1 plays a specific role in DNA replication, promoting the Srs2 recruitment to PCNA independently of checkpoint signaling. Srs2 would prevent formation of double Holliday junctions (dHJs) and thus CO formation. Sgs1 also negatively regulates CO formation but through a different process that resolves dHJs to yield non-CO products.
authors.affiliation: Commissariat à l'Energie Atomique, UMR217 CNRS-CEA/DSV/DRR/SRMC/LERA, Fontenay-aux-roses, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Robert
authors.forename: Delphine
authors.initials: D
authors.lastname: Dervins
authors.forename: Francis
authors.initials: F
authors.lastname: Fabre
authors.forename: Serge
authors.initials: S
authors.lastname: Gangloff
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D015825:Chromosomes, Fungal / Q000235:genetics; D003434:Crossing Over, Genetic; D004249:DNA Damage / Q000235:genetics; D004265:DNA Helicases / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D004720:Endonucleases / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics; D008938:Mitosis; D008957:Models, Genetic; D009154:Mutation / Q000235:genetics; D010766:Phosphorylation; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D053484:RecQ Helicases; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C441579:MRC1 protein, S cerevisiae; D018809:Proliferating Cell Nuclear Antigen; D029701:Saccharomyces cerevisiae Proteins; C502374:TOP3 protein, S cerevisiae; C061376:SRS2 protein, S cerevisiae; D004720:Endonucleases; C411624:MUS81 protein, S cerevisiae; C090472:SGS1 protein, S cerevisiae; D004265:DNA Helicases; D053484:RecQ Helicases