title: Characterization of acatalasemic erythrocytes treated with low and high dose hydrogen peroxide. Hemolysis and aggregation.
abstract: The effects of hydrogen peroxide on normal and acatalasemic erythrocytes were examined. Severe hemolysis of acatalasemic erythrocytes and a small tyrosine radical signal (g = 2.005) associated with the formation of ferryl hemoglobin were observed upon the addition of less than 0.25 mM hydrogen peroxide. However, when the concentration of hydrogen peroxide was increased to 0.5 mM, acatalasemic erythrocytes became insoluble in water and increased the tyrosine radical signal. Polymerization of hemoglobin and aggregation of the erythrocytes were observed. On the other hand, normal erythrocytes exhibited only mild hemolysis by the addition of hydrogen peroxide under similar conditions. From these results, the scavenging of hydrogen peroxide by hemoglobin generates the ferryl hemoglobin species (H-Hb-Fe(IV)=O) plus protein-based radicals (*Hb-Fe(IV)=O). These species induce hemolysis of erythrocytes, polymerization of hemoglobin, and aggregation of the acatalasemic erythrocytes. A mechanism for the onset of Takarara disease is proposed.
authors.affiliation: Department of Life Science, Okayama University of Science, 700-0005; Department of Biochemistry, Okayama University Graduate School of Medicine and Dentistry, 700-8558. Electronic address: masuokan@dls.ous.ac.jp.
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Masuoka
authors.affiliation: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine and Dentistry, 700-8558.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sugiyama
authors.affiliation: Okayama University Medical School, 700-8558.
authors.forename: Naoki
authors.initials: N
authors.lastname: Ishibashi
authors.affiliation: Department of Public Health, Okayama University Graduate School of Medicine and Dentistry, 700-8558.
authors.forename: Da-Hong
authors.initials: DH
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Science, Okayama University, 700-8530.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Masuoka
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Kochi University Medical School, 783-8505.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kodama
authors.affiliation: Department of Pharmaceutical Science, University of Toyama, 930-0194, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Nakano
mesh_terms: D020642:Acatalasia / Q000097:blood* / Q000209:etiology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology*; D006454:Hemoglobins; D006461:Hemolysis / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011108:Polymers
chemical_list: D006454:Hemoglobins; D011108:Polymers; D006861:Hydrogen Peroxide