Genotyping and mapping with high-density oligonucleotide arrays.
This chapter discusses the specific details of how oligonucleotide arrays originally designed for expression analysis can be used for high-density genotyping to reveal tens of thousands of single-feature polymorphisms (SFPs). Cluster of SFPs reveal potential natural deletion polymorphisms. SFPs can be readily used for mapping mutations using bulk segregant analysis. An example is given using the Columbia and Landsberg erecta strains of Arabidopsis thaliana.
Borevitz
Justin
J
Ecology and Evolution, University of Chicago, IL, USA.
D017360:Arabidopsis / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017343:Genes, Plant; D005838:Genotype; D008957:Models, Genetic; D009154:Mutation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D009841:Oligonucleotides