Anti-inflammatory effect of amurensin H on asthma-like reaction induced by allergen in sensitized mice.
OBJECTIVE
To explore the anti-inflammatory effects of amurensin H on asthma-like reaction induced by allergen in sensitized mice.


METHODS
BALB/c mice were sensitized by ovalbumin (OVA, ip) on d 0 and d 14 and challenged with 1% OVA on d 18 to 22. Mice developed airway eosinophilia, mucus hypersecretion, and elevation in cytokine levels. Mice were administered amurensin H orally at the doses of 49, 70, or 100 mg/kg once every day from d 15 to the last day. Bronchoalveolar lavage fluid (BALF) were collected at 24 h and 48 h after the last OVA challenge. Levels of tumor necrosis factor-alpha (TNF-alpha), interleukin 4 (IL-4), interleukin 5 (IL-5), and interleukin 13 (IL-13) in BALF were measured using ELISA method. Differential cell counts of macrophages, lymphocytes, neutrophils and eosinophils were performed in 200 cells per slide (one slide per animal). Lung tissue sections of 6-mum thickness were stained with Mayer's hematoxylin and eosin for assessment of cell infiltration, mucus production, and tissue damage.


RESULTS
Oral administration of amurensin H significantly inhibited OVA-induced increases in total cell counts, eosinophil counts, and TNF- alpha, IL-4, IL-5 and IL-13 levels in BALF. In addition, amuresin H dramatically decreased OVA-induced lung tissue damage and mucus production.


CONCLUSIONS
Amurensin H may have therapeutic potential for the treatment of allergic airway inflammation.
Li
Yi-Tang
YT
Department of Pharmacology, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China.
Yao
Chun-Suo
CS
Bai
Jin-Ye
JY
Lin
Mao
M
Cheng
Gui-Fang
GF
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010047:Ovalbumin; D010946:Plants, Medicinal / Q000737:chemistry; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D027843:Vitis / Q000737:chemistry
D000893:Anti-Inflammatory Agents; D001572:Benzofurans; D016207:Cytokines; D018793:Interleukin-13; D015848:Interleukin-5; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; C521077:amurensin H; D015847:Interleukin-4; D010047:Ovalbumin