Molecular fingerprinting of Vibrio tapetis strains using three PCR-based methods: ERIC-PCR, REP-PCR and RAPD.
Brown Ring Disease (BRD) is a bacterial disease caused by Vibrio tapetis which affects cultured clams and causes heavy economic losses. In this study, 28 V. tapetis strains isolated from 5 different hosts were intraspecifically characterized by 3 different polymerase chain reaction- (PCR-) based typing methods: enterobacteria repetitive intergenic consensus (ERIC)-PCR, repetitive extragenic palindromic (REP)-PCR and randomly amplified polymorphic DNA (RAPD)-PCR. Cluster analysis of genetic profiles obtained from these molecular techniques clearly showed the existence of 3 genetic groups strongly correlated to the host origin. The first group was formed by 23 V. tapetis strains isolated from Manila clam Ruditapes philippinarum, 1 isolated from venus clam Venerupis aurea, and 1 isolated from common cockle Cerastoderma edule, all collected from France and Spain. The second group was formed by 2 strains isolated from carpet-shell clam R. decussatus cultured in the northwest of Spain. The third group was composed of 1 strain isolated from Atlantic halibut Hippoglossus hippoglossus from the UK. We concluded that the 3 typing methods based on PCR were useful for the intraspecific typing of V. tapetis strains, and that they can potentially be used as a fast and reliable tool for epidemiological studies in the future.
Rodríguez
José M
JM
Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago de Compostela, Spain.
López-Romalde
Sonia
S
Beaz
Roxana
R
Alonso
M Carmen
MC
Castro
Dolores
D
Romalde
Jesús L
JL
D000818:Animals; D049872:Bivalvia / Q000382:microbiology; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005432:Flounder / Q000382:microbiology; D014644:Genetic Variation / Q000235:genetics; D017720:Molecular Epidemiology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D014733:Vibrio / Q000145:classification* / Q000235:genetics*
D017931:DNA Primers; D004269:DNA, Bacterial