Substrate porosity enhances chondrocyte attachment, spreading, and cartilage tissue formation in vitro.
Tissue engineering is being explored as a new approach to treat damaged cartilage. As the biomaterial used may influence tissue formation, the effects of substrate geometry on chondrocyte behavior in vitro were examined. Articular chondrocytes were isolated and cultured on the surface of smooth, rough, porous-coated, and fully porous Ti-6Al-4V substrates. The percentage of chondrocytes that attached to each substrate at 24 h was determined. After 24 and 72 h, chondrocytes were visualized by scanning electron microscopy and cell areas were measured. Collagen and proteoglycan accumulation within the first 24 h was determined by incorporation with [3H]-proline and [35S]-SO4, respectively. Chondrocyte attachment as well as matrix accumulation was enhanced as substrate surface area increased. Cell areas on the fully porous substrate were over four times greater than on any other substrate by 72 h in culture. After 8 weeks in culture, a continuous layer of cartilaginous tissue formed only on the surface of the fully porous substrate. This suggests that fully porous Ti-6Al-4V substrates provide the conditions that favor cartilage tissue formation by influencing cell attachment and extent of cell spreading. Understanding how substrate porosity influences chondrocyte behavior may help identify methods to further enhance cartilage tissue formation in vitro.
Spiteri
C G
CG
Bioengineering of Skeletal Tissues Team, Pathology and Laboratory Medicine, Mount Sinai Hospital, 600 University Avenue, Suite 600-9, Toronto, Ontario, Canada M5G 1X5.
Pilliar
R M
RM
Kandel
R A
RA
D000497:Alloys; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000648:ultrastructure; D002417:Cattle; D002448:Cell Adhesion; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000648:ultrastructure; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D014025:Titanium
D000497:Alloys; C031462:titanium alloy (TiAl6V4); D014025:Titanium