title: The in vitro antiviral activity of an aliphatic nitro compound from Heteropteris aphrodisiaca.
abstract: We investigated the antiviral activity of an aliphatic nitro compound (NC) isolated from Heteropteris aphrodisiaca O. Mach. (Malpighiaceae), a Brazilian medicinal plant. The NC was tested for its antiviral activity against poliovirus type 1 (PV-1) and bovine herpes virus type 1 (BHV-1) by plaque reduction assay in cell culture. The NC showed a moderate antiviral activity against PV-1 and BHV-1 in HEp-2 cells, and the 50% inhibitory concentration (IC50) were 22.01 microg/ml (selectivity index (SI)=2.83) and 21.10 microg/ml (SI=2.95), respectively. At the highest concentration of the drug (40 microg/ml) a reduction of approximately 80% in plaque assay was observed for both viruses. The treatment of cells or virus prior to infection did not inhibit the replication of virus strains.
authors.affiliation: Departamento de Microbiologia/CCB, Universidade Estadual de Londrina, Caixa Postal 6001, CEP 86051-990 Londrina-Paraná, Brazil.
authors.forename: Fernando L
authors.initials: FL
authors.lastname: Melo
authors.forename: Fabricio J
authors.initials: FJ
authors.lastname: Benati
authors.forename: Walter Antonio
authors.initials: WA
authors.lastname: Roman
authors.forename: João Carlos Palazzo
authors.initials: JC
authors.lastname: de Mello
authors.forename: Carlos
authors.initials: C
authors.lastname: Nozawa
authors.forename: Rosa Elisa Carvalho
authors.initials: RE
authors.lastname: Linhares
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001938:Brazil; D002417:Cattle; D045744:Cell Line, Tumor; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D007242:Herpesvirus 1, Bovine / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D009574:Nitro Compounds / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D017955:Poliovirus / Q000187:drug effects*; D010948:Viral Plaque Assay
chemical_list: D000998:Antiviral Agents; D009574:Nitro Compounds; D010936:Plant Extracts