title: Dimethyl-acetamide as a cryoprotectant for rainbow trout spermatozoa.
abstract: Dimethyl-acetamide was evaluated as a cryoprotectant for rainbow trout (Oncorhynchus mykiss ) semen on the basis of motility, percentage of dead spermatozoa as determined by fluorometry, and fertility of frozen-thawed spermatozoa. Dimethyl-acetamide performed significantly better (P<0.05) than the conventional cryoprotectant, dimethyl sulfoxide, by all evaluation methods. An extender comprised of 0.137 M NaCl, 0.011 M KCl, 0.004 M Na(2)HPO(4) 7H(2)O, 7.5 g/l L-alpha-lecithin and 10 % DMA showed promise for cryopreserving rainbow trout spermatozoa. Future evaluation of new extenders should be carried out by several in vitro techniques and fertility measurements to present a complete assessment of an extender's ability to cryopreserve sperm cells.
authors.affiliation: Department of Health Management Atlantic Veterinary College University of Prince Edward Island Charlottetown, P.E.I., Canada, C1A 4P3.
authors.forename: M A
authors.initials: MA
authors.lastname: McNiven
authors.forename: R K
authors.initials: RK
authors.lastname: Gallant
authors.forename: G F
authors.initials: GF
authors.lastname: Richardson