Separation of CD34+ cells from human peripheral blood through polyurethane foaming membranes.
Cell separation from peripheral blood was investigated using polyurethane (PU) foaming membranes and PU membranes (pore size, 5 or 12 mum) at different blood permeation speeds. Permeation ratio of hematopoietic stem cells (CD34(+) cells) through the PU membranes was the lowest among the blood cells at any blood permeation speed. This is thought to be because CD34(+) cells are more adhesive than red blood cells (RBCs), platelets, T cells, and B cells. Primitive hematopoietic stem and progenitor cells tend to adhere to the surface of mature blood cells, because of the high expression of cell-adhesion molecules on the surface of the cells. Human serum albumin solution was exposed to PU-COOH membranes to detach adhered cells from the surface of the membranes, allowing isolation of CD34(+) cells and reduction of RBCs in the permeate solution. Most purified CD34(+) cells (high recovery ratio of CD34(+) cells divided by recovery ratio of RBCs) were obtained in the recovery process using PU-COOH membranes (pore size, 5.2 microm) at a permeation speed of 0.3-1 mL/min.
Higuchi
Akon
A
Department of Applied Chemistry, Seikei University, 3-1 Kichijoji Kitamachi 3, Musashino, Tokyo 180-8633, Japan. higuchi@st.seikei.ac.jp
Iizuka
Ayumi
A
Gomei
Yumiko
Y
Miyazaki
Toyohiko
T
Sakurai
Masaru
M
Matsuoka
Yuki
Y
Natori
Shizue Hayashi
SH
D000328:Adult; D018952:Antigens, CD34 / Q000097:blood*; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004912:Erythrocytes / Q000648:ultrastructure; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007962:Leukocytes / Q000648:ultrastructure; D008567:Membranes, Artificial; D011140:Polyurethanes
D018952:Antigens, CD34; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011140:Polyurethanes