title: Studies on lactic dehydrogenase and sorbitol dehydrogenase release in relation to deep freezing of buffalo semen in certain extenders.
abstract: Forty seminal ejaculates from five mature buffalo bulls (n=8 from each bull), exhibiting more than 70% initial sperm motility, were frozen in the following three extenders: egg-yolk sodium citrate glycerol (EYCG); tris-egg yolk glycerol (TYG); and citric acid whey glycerol (CAWG). The extenders were evaluated for the release of intracellular enzymes, lactic dehydrogenase (LDH) and sorbitol dehydrogenase (SODH) from the spermatozoa during freezing (in fresh semen, after dilution and after equilibration) and post freezing (24 h and 7 d after freezing. It was found that the release of LDH and SODH enzymes was significantly lower in TYG than in EYCG and CAWG extenders. The most critical stage, at which the enzyme release was maximal, was between equilibration and 24 h post freezing in all three extenders.
authors.affiliation: Department of Veterinary Obstetrics and Gynaecology, College of Veterinary Science, Punjab Agricultural University, Ludhiana - 141 004, India.
authors.forename: J
authors.initials: J
authors.lastname: Singh
authors.forename: G R
authors.initials: GR
authors.lastname: Pangawkar
authors.forename: R K
authors.initials: RK
authors.lastname: Biswas
authors.forename: A K
authors.initials: AK
authors.lastname: Srivastava
authors.forename: R D
authors.initials: RD
authors.lastname: Sharma