title: The reducing antioxidant capacity of Mycoplasma fermentans.
abstract: Mycoplasma fermentans is an extracellular microorganism capable of adhering to the surface of host cells. It has been recently shown that plasminogen binding to M. fermentans in the presence of the urokinase-type plasminogen activator promotes the invasion of host cells by this organism. In this report, we show that viable mycoplasmas persist within the infected HeLa cells for prolonged periods of time despite the expectation that within host cells the organism may be exposed to oxidative stress. Using cyclic voltammetry and luminol-enhanced chemiluminescence assays, we detected a potent reducing antioxidant activity in M. fermentans. The reducing antioxidant activity was heat stable, not affected by proteolysis and was almost totally lost upon dialysis suggesting that the activity is due to a nonproteinaceus low molecular weight antioxidant. This antioxidant was partially purified by Bio-Gel column chromatography followed by high-pressure liquid chromatographic analysis. We suggest that the high reducing antioxidant capacity in M. fermentans is a principal defense mechanism playing a major role in the battle of the organism against oxidative stress within the host cells.
authors.affiliation: Department of Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: Amichai
authors.initials: A
authors.lastname: Yavlovich
authors.forename: Ron
authors.initials: R
authors.lastname: Kohen
authors.forename: Isaac
authors.initials: I
authors.lastname: Ginsburg
authors.forename: Shlomo
authors.initials: S
authors.lastname: Rottem
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002458:Cell Fractionation; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006367:HeLa Cells; D006801:Humans; D008163:Luminescent Measurements; D008970:Molecular Weight; D016858:Mycoplasma fermentans / Q000378:metabolism* / Q000472:pathogenicity; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D014774:Virulence
chemical_list: D000975:Antioxidants