title: Safety evaluation of hypoallergenic wheat flour by using a DNA microarray.
abstract: The effect of the consumption of hypoallergenic wheat flour (HWF) on the expression of a wide spectrum of genes was analyzed by using a DNA microarray. Gene expression profiles in the liver and intestines of rats fed on a diet composed mainly of HWF were compared with those of rats fed on a normal flour-based diet. Among the eight thousand transcripts represented on the GeneChip microarray, no more than 30 genes exhibited up- or down-regulation of two-fold or more after one week or two months of HWF consumption. No adverse effects were apparent. Up-regulation of some of the genes known to respond to the interferon-gamma signal was apparent in the one-week experiment, which may be related to possible oral immunotolerance resulting from HWF feeding. This DNA microarray technology presents an efficient method for evaluating the safety of foods.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Narasaka
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Endo
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Fu
authors.forename: Megumi
authors.initials: M
authors.lastname: Moriyama
authors.forename: Soichi
authors.initials: S
authors.lastname: Arai
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.forename: Hisanori
authors.initials: H
authors.lastname: Kato
mesh_terms: D000485:Allergens / Q000494:pharmacology*; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D005433:Flour / Q000592:standards*; D007421:Intestine, Small / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D014908:Triticum; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000485:Allergens