title: Psychrophilic trypsin-type protease from Serratia proteamaculans.
abstract: A preparative method for purification of a novel protease from the psychrotolerant Gram-negative microorganism Serratia proteamaculans (PSP) was developed using affinity chromatography on BPTI-Sepharose. It yielded electrophoretically homogeneous PSP preparation of 60 kD. The PSP properties (temperature and pH stability, high catalytic efficiency) indicate that this enzyme can be defined as a psychrophilic protease. Inhibitory analysis together with substrate specificity indicates that the studied PSP exhibits properties of serine trypsin-like and Zn-dependent protease.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. anna@enzyme.siobc.ras.ru
authors.forename: A G
authors.initials: AG
authors.lastname: Mikhailova
authors.forename: V V
authors.initials: VV
authors.lastname: Likhareva
authors.forename: R F
authors.initials: RF
authors.lastname: Khairullin
authors.forename: N L
authors.initials: NL
authors.lastname: Lubenets
authors.forename: L D
authors.initials: LD
authors.lastname: Rumsh
authors.forename: I V
authors.initials: IV
authors.lastname: Demidyuk
authors.forename: S V
authors.initials: SV
authors.lastname: Kostrov
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004795:Enzyme Stability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D012705:Serratia / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature; D014357:Trypsin / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011480:Protease Inhibitors; D010447:Peptide Hydrolases; D014357:Trypsin