title: Lysozyme, alkaline phosphatase and neutrophils in uterine secretions of mares with differing resistance to endometritis.
abstract: A study was conducted to 1) determine differences in the inflammatory response following bacterial challenge between normal mares and mares with chronic endometritis and 2) to determine if enzyme activity in uterine fluid can be used to evaluate degree of inflammation in the equine uterus. Six normal mares (Group 1) and four mares with chronic endometritis (Group 2) received an intrauterine infusion of beta-hemolytic streptococci on the second day of estrus. Neutrophil concentration as well as lysozyme and alkaline phosphatase activity were determined in uterine secretions obtained by placing tampons in the uterus of mares. All mares had a similar inflammatory response following bacterial challenge of the uterus, as indicated by a neutrophil response of the same magnitude. Neutrophil numbers, lysozyme and alkaline phosphatase concentrations were all increased 12 h postinoculation and declined rapidly to normal preinoculation values by 48 h after inoculation. In spite of the similarity of the clinical signs, neutrophil concentrations and enzyme activity, mares in group 1 demonstrated a markedly higher ability to eliminate the infection than mares in group 2. It is concluded that factors other than neutrophil numbers, lysozyme and alkaline phosphatase activity account for the inability of the mare to eliminate uterine infections.
authors.affiliation: Department of Veterinary Clinical Medicine University of Illinois Urbana, IL 61801 USA.
authors.forename: T
authors.initials: T
authors.lastname: Katila
authors.forename: T F
authors.initials: TF
authors.lastname: Lock
authors.forename: W E
authors.initials: WE
authors.lastname: Hoffmann
authors.forename: A R
authors.initials: AR
authors.lastname: Smith