Routine immunophenotyping in acute leukemia: Role in lineage assignment and reassignment.
Diagnostic evaluation of acute leukemia at Roswell Park Cancer Institute has routinely included immunophenotyping by multiparameter flow cytometry. In a retrospective analysis of 646 cases, morphology and cytochemistry established lineage in 612, but not in 34 (5%), of which 26, 5, and 3 were myeloid, undifferentiated, and lymphoid, respectively, based on immunophenotyping. In addition, immunophenotyping changed the lineage assigned based on morphology and cytochemistry in 11 cases (2%); 8 changed from lymphoid to myeloid, and 3 from myeloid to lymphoid. The data support routine inclusion of at least limited immunophenotyping in the diagnostic evaluation of acute leukemia.
Qadir
Misbah
M
Leukemia Section, Department of Medicine, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Barcos
Maurice
M
Stewart
Carleton C
CC
Sait
Sheila N J
SN
Ford
Laurie A
LA
Baer
Maria R
MR
D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis; D019070:Cell Lineage; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000145:classification* / Q000175:diagnosis*; D012189:Retrospective Studies
D015703:Antigens, CD; D014408:Biomarkers, Tumor