title: Immunization with the biologically active lectin domain of PapGII induces strong adhesion-inhibiting antibody responses but not protection against avian pathogenic Escherichia coli.
abstract: The aim of this study was to investigate whether immunization with the sugar binding domain of PapGII (PapGII196) was able to protect chickens against avian pathogenic Escherichia coli. PapGII196 was expressed, purified by Ni-NTA column chromatography and shown to retain its biological activity, as demonstrated by binding to its receptor, globoside. PapGII196 was tested as a vaccine in specific pathogen free broilers and also by vaccinating breeders and assessing protection in their offspring, and using aerosol exposure or air sac inoculation for challenge. Notwithstanding a strong anti-PapGII196 serum IgG response in vaccinated birds in all experiments and inhibition of haemagglutination by serum from PapGII196-vaccinated birds, chickens were not protected against avian pathogenic E. coli. These findings suggest that PapGII may not be a useful candidate for inclusion in vaccines against avian pathogenic E. coli.
authors.affiliation: Laboratory of Livestock Physiology and Immunology, Faculty of Bioscience Engineering, Catholic University Leuven, Kasteelpark Arenberg 30, 3001, Leuven, Belgium. Frederic.vandemaele@gmail.com
authors.forename: Fréderic
authors.initials: F
authors.lastname: Vandemaele
authors.forename: Nele
authors.initials: N
authors.lastname: Bleyen
authors.forename: Omran
authors.initials: O
authors.lastname: Abuaboud
authors.forename: Ed
authors.initials: E
authors.lastname: vanderMeer
authors.forename: Anton
authors.initials: A
authors.lastname: Jacobs
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Goddeeris
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D001422:Bacterial Adhesion / Q000276:immunology*; D002645:Chickens; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D022361:Escherichia coli Vaccines / Q000276:immunology*; D005260:Female; D006384:Hemagglutination; D007074:Immunoglobulin G / Q000097:blood; D037102:Lectins / Q000737:chemistry*; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D017434:Protein Structure, Tertiary; D013047:Specific Pathogen-Free Organisms
chemical_list: D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; D022361:Escherichia coli Vaccines; D007074:Immunoglobulin G; D037102:Lectins; C519334:PapGII protein, E coli