title: Effect of caffeine on the post-thaw motility of buffalo spermatozoa.
abstract: Buffalo semen was diluted (1:2) with lactose diluent containing caffeine (2, 4 and 6 mM). Diluted semen samples were frozen in a pellet form (0.15 ml), thawed 24 h after freezing in 2.9% sodium citrate for 30 sec and incubated at 37 degrees C for 3 h. Addition of caffeine to diluted buffalo semen before freezing resulted in a significant increase in the post-thaw motility of spermatozoa over the 3-h incubation period. When caffeine was added to the thawing medium, the post-thaw motility was further improved. Thus, the increase in motility due to caffeine treatment was even more pronounced than in samples treated with caffeine before freezing.
authors.affiliation: Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza-12211, Egypt.
authors.forename: El-S M
authors.initials: el-SM
authors.lastname: Fattouh
authors.forename: M S
authors.initials: MS
authors.lastname: Abdou