MultiPrimer: software for multiplex primer design.
This study presents a novel application, MultiPrimer, to assist users to design multiplex PCR primers. MultiPrimer adopts an algorithm based on a genetic algorithm and can efficiently solve the multi-objective optimisation problem. A match pattern model is proposed to speed up examination of the specificity constraint. MultiPrimer is able to find a set of primer pairs simultaneously for a set of gene family sequences and multiple targets for a single cDNA sequence. A public version of the MultiPrimer software is available from http://edith.cse.nsysu.edu.tw/new/software/MultiPrimer.htm.
Lee
Chungnan
C
Department of Computer Science and Engineering, National Sun Yat-Sen University, Kaohsiung, Taiwan. cnlee@mail.cse.nsysu.edu.tw
Wu
Jain-Shing
JS
Shiue
Yow-Ling
YL
Liang
Hong-Long
HL
D000465:Algorithms; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D017931:DNA Primers / Q000737:chemistry*; D018076:DNA, Complementary / Q000378:metabolism; D023281:Genomics / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D014584:User-Computer Interface
D017931:DNA Primers; D018076:DNA, Complementary