title: Fatal transfusion-associated graft-versus-host disease in an immunocompetent recipient of a volunteer unit of red cells.
abstract: BACKGROUND
  Transfusion-associated graft-versus-host disease (TAGVHD) is a lethal complication of transfusion of nonirradiated cellular blood components to a susceptible recipient.
  
  
  METHODS
  An 82-year-old man underwent cardiac surgery during which he received 6 units of red cells (RBCs) and a 6-unit pool of platelets (PLTs). He was discharged with a normal white blood cell (WBC) count and hemoglobin (Hb) level and a PLT count of 104x10(9) per L. He was readmitted 2 weeks later with a diffuse erythematous rash, a sore throat, and difficulty swallowing. His WBC count was 2.1x10(9) per L, his Hb level was 12.0 g per dL, and his PLT count was 131x10(9) per L. The next day he had worsening cytopenias: WBC count, 1x10(9) per L; Hb level, 10.9 g per dL; PLT count, 104x10(9) per L. He also had diarrhea. A marrow biopsy showed a severe hypoplasia without evidence of malignancy. A skin biopsy showed Grade II GVHD. The patient worsened and despite aggressive therapy he expired on Postoperative Day 42. DNA-based HLA testing of the 12 blood donors was performed. One of the RBC donors was found to be homozygous for an HLA Class I and Class II haplotype in the patient.
  
  
  CONCLUSIONS
  This is the first reported case in the United States of fatal TAGVHD from RBCs in an immunocompetent patient who received a randomly selected unit of RBCs from a donor who was homozygous for a shared HLA haplotype. The policy of selective irradiation should be reexamined.
authors.affiliation: Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA. dtriulzi@itxm.org
authors.forename: Darrell
authors.initials: D
authors.lastname: Triulzi
authors.forename: Rene
authors.initials: R
authors.lastname: Duquesnoy
authors.forename: Lawrence
authors.initials: L
authors.lastname: Nichols
authors.forename: Kenneth
authors.initials: K
authors.lastname: Clark
authors.forename: Drazen
authors.initials: D
authors.lastname: Jukic
authors.forename: Adriana
authors.initials: A
authors.lastname: Zeevi
authors.forename: Dennis
authors.initials: D
authors.lastname: Meisner
mesh_terms: D000368:Aged; D001782:Blood Donors; D017707:Erythrocyte Transfusion / Q000009:adverse effects*; D017809:Fatal Outcome; D006086:Graft vs Host Disease / Q000209:etiology* / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics; D006648:Histocompatibility / Q000235:genetics; D006720:Homozygote; D006801:Humans; D016867:Immunocompromised Host; D008297:Male
chemical_list: D006680:HLA Antigens