title: In vitro fertilizing capacity of frozen-thawed boar semen.
abstract: We describe a porcine semen cryopreservation technique and assess the in vitro fertilizing capacity of the frozen-thawed spermatozoa. The thawed spermatozoa did not lose the physiological properties of motility, viability, and acrosome reaction or capacity to fertilize in vitro. Immediately after thawing, the spermatozoa showed 51% mean motility, 60% viability, and 5% induced acrosome reaction. After 2.5 h of incubation in TALP medium, the spermatozoa exhibited 61% motility, 63% viability and 40% induced acrosome reaction. The average in vitro fertilization capacity of thawed spermatozoa was 68% compared with that of spermatozoa from fresh semen (85%). The percentage of polyspermy was highly variable, with frozen-thawed samples ranging from 0 to 28% and fresh samples from 0 to 30%. The results obtained with frozen semen from 5 boars of different breeds did not show considerable variation. This suggests that the freezing-thawing technique is reproducible and adequate for in vitro fertilization.
authors.affiliation: Department of Agriculture and Animal Production, Metropolitan Autonomous University-Xochimilco, México, D.F. México.
authors.forename: A
authors.initials: A
authors.lastname: Córdova
authors.forename: Y
authors.initials: Y
authors.lastname: Ducolomb
authors.forename: I
authors.initials: I
authors.lastname: Jiménez
authors.forename: E
authors.initials: E
authors.lastname: Casas
authors.forename: E
authors.initials: E
authors.lastname: Bonilla
authors.forename: M
authors.initials: M
authors.lastname: Betancourt