The use of lectins to characterize and separate living canine spermatozoa: A preliminary study.
This study was designed as a preliminary attempt to develop a methodology for relating the glucidic structure of the sperm membrane to sperm morphology. Differences in plasma membrane glycoconjugates between motile and nonmotile spermatozoa were studied by using 7 lectins. Fresh spermatozoa from 3 dogs were analyzed by fluorescein isothiocyanate (FITC) labeled lectins. The binding of lectins to the sperm membrane and the capability of the lectins to agglutinate spermatozoa were estimated semi-quantitatively by observation with either an epifluorescence or a phase contrast microscope, respectively. All the lectins tested bound to non motile spermatozoa, with Helix pomatia , Pisum sativum and Arachis hypogaea showing intense fluorescence, Triticum vulgare and Glycine maxima showing moderate fluorescence, and Phaseolus vulgaris and Phytolacca americana showing low fluorescence. However, Helix pomatia . and Triticum vulgare also bound to rapid and slow moving spermatozoa, and were the only 2 lectins that induced sperm agglutination. These results suggest that lectins could be a possible tool for characterizing and separating spermatozoa with different rates of motility.
González-Chábarri
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Dep. Producción Animal, Fac. de Veterinaria, Madrid, Spain.
Aguado
J A
JA
Navarro
M
M
López-Rodas
V
V
Costas
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