title: [Progress of the PCR amplification techniques for chromosome walking].
abstract: Various PCR-based methods are available for chromosome walking from a known sequence to an unknown region. It is promising in genome-related research for the following experiments: promoter cloning, obtaining non-conservative parts of genes in new species, identification of T-DNA or transposon insertion sites and filling in gaps or unknown chromosome regions in genome sequencing. These methods consisted of three types: inverse PCR, ligation-mediated PCR and specific primer PCR. In this review, we illustrated and compared the current techniques.
authors.affiliation: School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116023, China. ppic9k@msn.com
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Qiao
authors.initials: Q
authors.lastname: Su
authors.forename: Min-Qian
authors.initials: MQ
authors.lastname: Tang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Yuan
authors.forename: Li-Jia
authors.initials: LJ
authors.lastname: An
mesh_terms: D000818:Animals; D016386:Chromosome Walking / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers