title: IL-10 and TNF-beta gene polymorphisms have no major influence on lactate levels after cardiac surgery.
abstract: OBJECTIVE
  Lactate levels after cardiac surgery are influenced by different proinflammatory (TNF, IL-6, IL-8) and anti-inflammatory (IL-10) cytokines. The goal of the study was to determine the relationship between polymorphism in the IL-10 (-1082G/A) and TNF-beta (+252G/A) genes and lactate levels in patients after cardiac surgery.
  
  
  METHODS
  We performed prospective observational study in 168 consecutive adult patients without left ventricle dysfunction undergoing elective coronary artery bypass grafting. Lactic acid levels were documented at five different time points: 10 min after beginning of cardiopulmonary bypass, 40 min after cardiopulmonary bypass termination, and 30 min, 8h, and 16 h after the surgery. Genetic analysis for polymorphism was performed by mismatched polymerase chain reaction and restriction analysis.
  
  
  RESULTS
  No association was found between single polymorphism in IL-10 or TNF-beta gene and lactate levels, but the carriers of IL-10/TNF-beta genotype combination +A/GG had significantly different course of lactate levels in time with decrease in lactate (in comparison with increase in other groups) at 8h after the surgery.
  
  
  CONCLUSIONS
  IL-10 (-1082G/A) and TNF-beta (+252G/A) gene polymorphisms have a little, yet measurable influence on the time course of changes in lactate levels after cardiac surgery.
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. hynek.riha@ikem.cz
authors.forename: Hynek
authors.initials: H
authors.lastname: Riha
authors.forename: Jaroslav A
authors.initials: JA
authors.lastname: Hubacek
authors.forename: Rudolf
authors.initials: R
authors.lastname: Poledne
authors.forename: Pavel
authors.initials: P
authors.lastname: Kellovsky
authors.forename: Ales
authors.initials: A
authors.lastname: Brezina
authors.forename: Jan
authors.initials: J
authors.lastname: Pirk
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001026:Coronary Artery Bypass; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000235:genetics; D018836:Inflammation Mediators / Q000097:blood; D016753:Interleukin-10 / Q000235:genetics*; D019344:Lactic Acid / Q000097:blood*; D008233:Lymphotoxin-alpha / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011184:Postoperative Period; D011446:Prospective Studies; D016277:Ventricular Function, Left
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators; D008233:Lymphotoxin-alpha; D016753:Interleukin-10; D019344:Lactic Acid