title: Nuclear abnormalities in erythrocytes of parrots (Aratinga canicularis) related to genotoxic damage.
abstract: Nuclear abnormalities in erythrocytes, as micronuclei and nuclear buds (BE), are considered potential biomarkers of genotoxic exposure. We described previously the frequency of spontaneous micronucleated erythrocytes (MNE) in the species Aratinga canicularis. Here, we have used this species to evaluate the induction of MNE and BE by mitomycin-C. Animals were given a single intracoelomic injection of 0, 2, 3 or 4 mg/kg mitomycin-C on two consecutive days. A drop of blood was obtained after 0, 24, 48 and 72 h, and stained smears were used to count micronucleated polychromatic erythrocytes (MNPCE) and polychromatic erythrocytes with buds (BPCE)/1000 polychromatic erythrocytes. The number of MNE and BE in 10 000 total erythrocytes was also counted. MNPCE and BPCE frequencies were elevated at 24, 48, and 72 h after the administration of the lower dose (P<0.03). At a 3 mg/kg dose, the frequency of MNPCE increased at 48 and 72 h (P<0.04) whereas the number of BPCE increased, but not significantly. Administration of 4 mg/kg mitomycin-C increased the number of MNE observed at 72 h (P<0.03), the number of MNPCE at 48 h (P<0.01) and 72 h (P<0.006), the BE frequency at 72 h (P<0.05), and the frequency of BPCE at 48 and 72 h (P<0.001). While mitomycin-C appears to produce a parallel increase in MNPCE and BPCE frequencies, the MNE seemed to be a more sensitive indicator of genotoxicity than the BE. This suggests that evaluating BE and MNE in routine haematological analysis should be considered to evaluate environmental genotoxic exposure.
authors.affiliation: Laboratorio de Mutagénesis, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Col. Independencia, C.P. 44340, Guadalajara, Jalisco, México. mutagenesis95@hotmail.com
authors.forename: Belinda C
authors.initials: BC
authors.lastname: Gómez-Meda
authors.forename: Ana L
authors.initials: AL
authors.lastname: Zamora-Perez
authors.forename: Jaime
authors.initials: J
authors.lastname: Luna-Aguirre
authors.forename: Andrés
authors.initials: A
authors.lastname: González-Rodríguez
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Ramos-Ibarra
authors.forename: Olivia
authors.initials: O
authors.lastname: Torres-Bugarín
authors.forename: Cecilia M
authors.initials: CM
authors.lastname: Batista-González
authors.forename: Guillermo M
authors.initials: GM
authors.lastname: Zúñiga-González
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects* / Q000235:genetics* / Q000473:pathology; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology*; D005260:Female; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000662:veterinary; D016685:Mitomycin / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity; D010311:Parrots / Q000097:blood / Q000235:genetics
chemical_list: D009153:Mutagens; D016685:Mitomycin