The CLAVATA3/ESR motif of CLAVATA3 is functionally independent from the nonconserved flanking sequences.
It is believed that CLAVATA3 (CLV3) encodes a peptide ligand that interacts with the CLV1/CLV2 receptor complex to limit the number of stem cells in the shoot apical meristem of Arabidopsis thaliana; however, the exact composition of the functional CLV3 product remains a mystery. A recent study on CLV3 shows that the CLV3/ESR (CLE) motif, together with the adjacent C-terminal sequence, is sufficient to execute CLV3 function when fused behind an N-terminal sequence of ERECTA. Here we show that most of the sequences flanking the CLE motif of CLV3 can be deleted without affecting CLV3 function. Using a liquid culture assay, we demonstrate that CLV3p, a synthetic peptide corresponding to the CLE motif of CLV3, is able to restrict the size of the shoot apical meristem in clv3 seedlings but not in clv1 seedlings. In accordance with this decrease in meristem size, application of CLV3p to in vitro-grown clv3 seedlings restricts the expression of the stem cell-promoting transcription factor WUSCHEL. Thus, we propose that the CLE motif is the functional region of CLV3 and that this region acts independently of its adjacent sequences.
Fiers
Martijn
M
Plant Research International, Centre for BioSystems Genomics, 6700 AA Wageningen, The Netherlands.
Golemiec
Elzbieta
E
van der Schors
Roel
R
van der Geest
Lonneke
L
Li
Ka Wan
KW
Stiekema
Willem J
WJ
Liu
Chun-Ming
CM
D020816:Amino Acid Motifs / Q000502:physiology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017124:Conserved Sequence; D017353:Gene Deletion; D005816:Genetic Complementation Test; D018398:Homeodomain Proteins / Q000378:metabolism; D018519:Meristem / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D018520:Plant Shoots / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
C118167:AT2G27250 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; C116421:WUSCHEL protein, Arabidopsis