Antineoplastic Agents. 554. The manitoba bacterium Streptomyces sp.
A Streptomyces sp. isolated from riverbank soil in Manitoba, Canada, was found to contain two cancer cell growth inhibitories: diazaanthraquinone 1 and 3-(hydroxyacetyl)indole (8). The structures were determined by interpretation of data from HRMS, UV, and high-field (400 MHz) NMR experiments. The red-colored diazaanthraquinone 1 and 3-(hydroxyacetyl)indole (8) were found to inhibit (0.1-3 microg/mL) growth of a minipanel of human cancer cell lines and P388 lymphocytic leukemia cells. Diazaanthraquinone 1 was also found to inhibit growth of the bacteria Streptococcus pneumoniae and Neisseria gonorrheae. However, three companion constituents, cyclo-Pro-Leu (5), cyclo-Pro-Phe (6), and cyclo-Pro-Val (7), did not inhibit cancer cell growth.
Pettit
George R
GR
Cancer Research Institute, Department of Chemistry and Biochemistry, Arizona State University, P.O. Box 872404, Tempe, Arizona 85287-2404, USA. bpettit@asu.edu
Du
Jiang
J
Pettit
Robin K
RK
Richert
Linda A
LA
Hogan
Fiona
F
Mukku
Venugopal J R V
VJ
Hoard
Michael S
MS
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001372:Aza Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004151:Dipeptides / Q000302:isolation & purification; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007941:Leukemia P388; D008350:Manitoba; D051379:Mice; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000302:isolation & purification; D011809:Quinones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013291:Streptococcus / Q000187:drug effects; D013302:Streptomyces / Q000737:chemistry* / Q000302:isolation & purification; D014407:Tumor Cells, Cultured
C511572:3-(hydroxyacetyl)indole; D000900:Anti-Bacterial Agents; D001372:Aza Compounds; D004151:Dipeptides; D007211:Indoles; D010456:Peptides, Cyclic; D011809:Quinones; C486843:Sch 538415; C505275:cyclo(prolyl-leucyl); C424926:cyclo(prolyl-valyl)