Functional role of matrix metalloproteinase-28 in the oral squamous cell carcinoma.
The newly identified MMP-28 has been shown to be expressed in several types of carcinomas, however, its functional role in transformation events is unknown. This study was to assess whether this proteinase plays a role in oral tumor malignancy. By using RT-PCR, we found that expression of MMP-28 was significantly higher in 92 oral squamous cell carcinomas (OSCCs) (52/92, 56.5%) than in seven oral premalignant lesions (OPMLs) (0/7, 0%) (P=0.004). No statistically significant correlation was found between MMP-28 expression and tumor stage, thickness, size, and metastasis. Both mRNA and protein of MMP-28 were preferentially concentrated in OSCC specimens than in neighboring tissues as analyzed by semi-quantitative RT-PCR (P=0.015) and immunohistochemistry, respectively. Transfection of OSCC and esophageal carcinoma cell lines with MMP-28 antisense oligodeoxynucleotide (AODN) resulted in the reduced secretion of MMP-28 protein and the ability of colony formation in soft agar without affecting cell growth. Our findings show the close correlation between MMP-28 and OSCC, and support a role for MMP-28 in the anchorage-independent growth of both OSCC and esophageal carcinomas.
Lin
Mei-Huei
MH
Department of Biotechnology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, ROC.
Liu
Shyun-Yeu
SY
Su
Hsiao-Jing
HJ
Liu
Young-Chau
YC
D015153:Blotting, Western / Q000379:methods; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D049109:Cell Proliferation; D018076:DNA, Complementary / Q000494:pharmacology; D004938:Esophageal Neoplasms / Q000201:enzymology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053505:Matrix Metalloproteinases, Secreted / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011230:Precancerous Conditions / Q000201:enzymology* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis / Q000378:metabolism; D014162:Transfection / Q000379:methods; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D009363:Neoplasm Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D019716:Tissue Inhibitor of Metalloproteinase-2; C424663:MMP28 protein, human; D053505:Matrix Metalloproteinases, Secreted