title: Tal1/Scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) embryonic stem cells.
abstract: The development of embryonic stem cell (ESC) therapies requires the establishment of efficient methods to differentiate ESCs into specific cell lineages. Here, we report the in vitro differentiation of common marmoset (CM) (Callithrix jacchus) ESCs into hematopoietic cells after exogenous gene transfer using vesicular stomatitis virus-glycoprotein-pseudotyped lentiviral vectors. We transduced hematopoietic genes, including tal1/scl, gata1, gata2, hoxB4, and lhx2, into CM ESCs. By immunochemical and morphological analyses, we demonstrated that overexpression of tal1/scl, but not the remaining genes, dramatically increased hematopoiesis of CM ESCs, resulting in multiple blood-cell lineages. Furthermore, flow cytometric analysis demonstrated that CD34, a hematopoietic stem/progenitor cell marker, was highly expressed in tal1/scl-overexpressing embryoid body cells. Similar results were obtained from three independent CM ESC lines. These results suggest that transduction of exogenous tal1/scl cDNA into ESCs is a promising method to induce the efficient differentiation of CM ESCs into hematopoietic stem/progenitor cells.
authors.affiliation: Department of Molecular Genetics, Division of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Fukuoka 812-8582, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Kurita
authors.forename: Erika
authors.initials: E
authors.lastname: Sasaki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yokoo
authors.forename: Takashi
authors.initials: T
authors.lastname: Hiroyama
authors.forename: Kashiya
authors.initials: K
authors.lastname: Takasugi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Imoto
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Izawa
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Takao
authors.initials: T
authors.lastname: Hashiguchi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Soda
authors.forename: Toyoki
authors.initials: T
authors.lastname: Maeda
authors.forename: Youko
authors.initials: Y
authors.lastname: Suehiro
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Yukoh
authors.initials: Y
authors.lastname: Nakazaki
authors.forename: Kenzaburo
authors.initials: K
authors.lastname: Tani
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology; D002144:Callithrix; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D004622:Embryo, Mammalian / Q000166:cytology*; D015870:Gene Expression / Q000187:drug effects; D005822:Genetic Vectors / Q000235:genetics; D006133:Growth Substances / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects; D014161:Transduction, Genetic
chemical_list: D018952:Antigens, CD34; D006133:Growth Substances; D011518:Proto-Oncogene Proteins