Electrophysiological recordings of single ion channels in planar lipid bilayers using a polymethyl methacrylate microfluidic chip.
Planar lipid bilayers are used for functional studies of ion channel proteins using electrophysiological techniques. We have been developing a plastic micro-fluidic device for the reconstitution of planar lipid bilayers and electrophysiological recordings toward a "membrane protein chip" for high-throughput screening. In the previous report [Suzuki, H., Tabata, K.V., Noji, H., Takeuchi, S., 2006. Highly reproducible method of planar lipid bilayer reconstitution in polymethyl methacrylate microfluidic chip. Langmuir 22 (4), 1937-1942], we presented the method and device in which the reproducibility of planar lipid bilayers reached 90%, and multiple bilayers were formed simultaneously. In this communication, we show that our device has excellent electric properties suitable for ion channel analysis down to single molecular level. Additional aspects on the optical accessibility and controllability on lipid bilayer formation are also presented.
Suzuki
Hiroaki
H
Institute of Industrial Science, The University of Tokyo, Meguro-ku, Tokyo 153-8505, Japan. hsuzuki@iis.u-tokyo.ac.jp
Tabata
Kazuhito V
KV
Noji
Hiroyuki
H
Takeuchi
Shoji
S
D004563:Electrochemistry / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D015640:Ion Channel Gating; D007473:Ion Channels / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008564:Membrane Potentials; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D019904:Polymethyl Methacrylate / Q000737:chemistry*
D007473:Ion Channels; D008051:Lipid Bilayers; D019904:Polymethyl Methacrylate