title: Autophosphorylation of alphaCaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse.
abstract: Autophosphorylation of alpha-Ca2+/calmodulin kinase II (alphaCaMKII) at Thr286 is thought to be a general effector mechanism for sustaining transcription-independent long-term potentiation (LTP) at pathways where LTP is NMDA receptor-dependent. We have compared LTP at two such hippocampal pathways in mutant mice with a disabling point mutation at the Thr286 autophosphorylation site. We find that autophosphorylation of alphaCaMKII is essential for induction of LTP at Schaffer commissural-CA1 synapses in vivo, but is not required for LTP that can be sustained over days at medial perforant path-granule cell synapses in awake mice. At these latter synapses LTP is supported by cyclic AMP-dependent signalling in the absence of alphaCaMKII signalling. Thus, the autophosphorylation of alphaCaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse.
authors.affiliation: MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK. scooke@nimr.mrc.ac.uk
authors.forename: Sam F
authors.initials: SF
authors.lastname: Cooke
authors.forename: Jianqun
authors.initials: J
authors.lastname: Wu
authors.forename: Florian
authors.initials: F
authors.lastname: Plattner
authors.forename: Michael
authors.initials: M
authors.lastname: Errington
authors.forename: Michael
authors.initials: M
authors.lastname: Rowan
authors.forename: Marco
authors.initials: M
authors.lastname: Peters
authors.forename: Ayumi
authors.initials: A
authors.lastname: Hirano
authors.forename: Karl D
authors.initials: KD
authors.lastname: Bradshaw
authors.forename: Roger
authors.initials: R
authors.lastname: Anwyl
authors.forename: Timothy V P
authors.initials: TV
authors.lastname: Bliss
authors.forename: K Peter
authors.initials: KP
authors.lastname: Giese
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D019943:Amino Acid Substitution; D000818:Animals; D054737:Calcium-Calmodulin-Dependent Protein Kinase Kinase; D002478:Cells, Cultured; D006624:Hippocampus / Q000502:physiology*; D017774:Long-Term Potentiation / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D017346:Protein Serine-Threonine Kinases; D054737:Calcium-Calmodulin-Dependent Protein Kinase Kinase