title: A short-term hypotonic treatment for chromosome preparation of intact and zona-penetrated mouse embryos.
abstract: An air-drying method for chromosome preparation was improved for use in zona-penetrated mouse embryos. This method consisted of a sequence of short-term (1 to 3 min) hypotonic treatments, a two-step fixation process and slow air-drying under heavy moisture. The method is also applicable to intact embryos at the one-cell, morula, and blastocyst stages for chromosome analysis of embryos at each of these stages.
authors.affiliation: Department of Animal Breeding & Reproduction, Faculty of Agriculture University of Utsunomiya, Mine-machi 350, Utsunomiya 321, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yoshizawa
authors.forename: S
authors.initials: S
authors.lastname: Nakamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Tsunoda
authors.forename: T
authors.initials: T
authors.lastname: Muramatsu