title: Isolation and characterization of four antibacterial peptides from bovine hemoglobin.
abstract: Peptic digestion of bovine hemoglobin at low degree of hydrolysis yields several intermediate peptide fractions after separation by reversed phase HPLC exhibiting antibacterial activity against Micrococcus luteus A270, Listeria innocua, Escherichia coli, and Salmonella enteritidis. From these fractions, four new antibacterial peptides were isolated and analyzed by ESI-MS/MS. Three of these peptides correspond to fragments of the alpha-chain of bovine hemoglobin: alpha107-141, alpha137-141, and alpha133-141, and one peptide to the beta-chain: beta126-145. The minimum inhibitory concentrations (MIC) of these peptides towards the four strains and their hemolytic activity towards bovine erythrocytes were determined.
authors.affiliation: Laboratoire de Procédés Biologiques Génie Enzymatique et Microbien, IUT A, Polytech'Lille-Lille I, BP 179, 59653 Villeneuve d'Ascq Cedex, France. Naima.Arroume@univ-lille1.fr
authors.forename: Naima
authors.initials: N
authors.lastname: Nedjar-Arroume
authors.forename: Véronique
authors.initials: V
authors.lastname: Dubois-Delval
authors.forename: Khalil
authors.initials: K
authors.lastname: Miloudi
authors.forename: Rachid
authors.initials: R
authors.lastname: Daoud
authors.forename: François
authors.initials: F
authors.lastname: Krier
authors.forename: Mostafa
authors.initials: M
authors.lastname: Kouach
authors.forename: Gilbert
authors.initials: G
authors.lastname: Briand
authors.forename: Didier
authors.initials: D
authors.lastname: Guillochon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D004723:Endorphins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006868:Hydrolysis; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004723:Endorphins; D006454:Hemoglobins; D010446:Peptide Fragments; C503955:hemoglobin (107-136), bovine; C036450:neo-kyotorphin