title: Intergeneric relationship between the Aspergillus ochraceous virus F and the Penicillium stoloniferum virus S.
abstract: It was reported that the "slow" component (PsV-S) of Penicillium stoloniferum virus complex also occurred in a second genus, Aspergillus ochraceous. The responsible virus for this intergeneric occurrence was considered to be the "fast" component (AoV-F) of A. ochraceous virus complex. In this investigation, AoV dsRNA 1, that was previously shown to cross-hybridize with PsV-S dsRNA, has been cloned. It was 1754 bp in length and contained one open reading frame of 539 amino acids (p63), had the same genome organization as PsV-S dsRNA S1 and also had the conserved sequence motif of the PsV-S dsRNAs (5'-GCGCAAAA-3') at the 5' terminus. A BLAST search indicated that p63 was a putative dsRNA-dependent RNA polymerase (RdRp), had 81% of sequence homology to members of the genus Partitivirus, and grouped together with PsV-S in phylogenetic analysis. But immunoblot analysis showed that the capsid protein (P3) of AoV-F virus component did not reacted against PsV-S antiserum. These evidences suggest that the cross serological relationship between AoV-F and PsV-S previously observed may have been due to the RdRps of the respective viruses rather than between their respective capsid proteins as was assumed in 1985.
authors.affiliation: Department of Biochemistry, College of Medicine, Inha University, Incheon 402-751, South Korea. jwkim@inha.ac.kr
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Kim
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Choi
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Kim
mesh_terms: D001235:Aspergillus ochraceus / Q000821:virology*; D004587:Electrophoresis, Agar Gel; D010407:Penicillium / Q000821:virology*; D010802:Phylogeny; D012328:RNA Viruses / Q000201:enzymology / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D017385:Sequence Homology; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase