Deoxypodophyllotoxin (DPT) inhibits eosinophil recruitment into the airway and Th2 cytokine expression in an OVA-induced lung inflammation.
The effect of deoxypodophyllotoxin (DPT) isolated from Anthriscus sylvestris Hoffm. was evaluated in an IN VIVO animal model for antiasthmatic activity. DPT (1.0 to 5 mg/kg) was given orally to ovalbumin (OVA)/alum-induced asthmatic mice. DPT reduced the number of infiltrated eosinophils in bronchoalveolar lavage (BAL) fluid in a dose-dependent manner. Dexamethasone (5 mg/kg), which was used as a positive control, also strongly inhibited the number of infiltrated eosinophils. The effect of DPT on a transcript profile in a murine asthma model was determined by RT-PCR, which showed that DPT decreased the mRNA levels of the Th2 cytokines. Northern blot analysis showed that DPT also reduced both the eotaxin and arginase I mRNA levels in a dose-dependent manner.
Lin
Chang Xin
CX
College of Pharmacy, Yeungnam University, Gyongsan, Korea.
Lee
Eunkyung
E
Jin
Mei Hua
MH
Yook
Jumin
J
Quan
Zhejiu
Z
Ha
Kyungmi
K
Moon
Tae Chul
TC
Kim
Mi Jin
MJ
Kim
Keuk Jun
KJ
Lee
Seung Ho
SH
Chang
Hyeun Wook
HW
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000737:chemistry / Q000494:pharmacology*; D019661:Apiaceae / Q000737:chemistry*; D001119:Arginase / Q000378:metabolism; D001249:Asthma / Q000276:immunology*; D002465:Cell Movement / Q000187:drug effects; D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004365:Drugs, Chinese Herbal; D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D028321:Plant Preparations / Q000494:pharmacology; D011014:Pneumonia / Q000276:immunology; D011034:Podophyllotoxin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects* / Q000276:immunology
D018927:Anti-Asthmatic Agents; C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D019742:Chemokines, CC; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D028321:Plant Preparations; D020033:Protein Isoforms; D012333:RNA, Messenger; C014451:deoxypodophyllotoxin; D010047:Ovalbumin; D001119:Arginase; D011034:Podophyllotoxin