title: Proteomic analysis of Brucella abortus cell envelope and identification of immunogenic candidate proteins for vaccine development.
abstract: Brucella abortus is the etiologic agent of bovine brucellosis and causes a chronic disease in humans known as undulant fever. In livestock the disease is characterized by abortion and sterility. Live, attenuated vaccines such as S19 and RB51 have been used to control the spread of the disease in animals; however, they are considered unsafe for human use and they induce abortion in pregnant cattle. For the development of a safer and equally efficacious vaccine, immunoproteomics was utilized to identify novel candidate proteins from B. abortus cell envelope (CE). A total of 163 proteins were identified using 2-DE with MALDI-TOF MS and LC-MS/MS. Some of the major protein components include outer-membrane protein (OMP) 25, OMP31, Omp2b porin, and 60 kDa chaperonin GroEL. 2-DE Western blot analyses probed with antiserum from bovine and a human patient infected with Brucella identified several new immunogenic proteins such as fumarate reductase flavoprotein subunit, F0F1-type ATP synthase alpha subunit, and cysteine synthase A. The elucidation of the immunome of B. abortus CE identified a number of candidate proteins for developing vaccines against Brucella infection in bovine and humans.
authors.affiliation: Vital Probes Inc., 1300 Old Plank Road, Mayfield, PA 18433, USA. jconnolly@vitalprobes.com
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Connolly
authors.forename: Diego
authors.initials: D
authors.lastname: Comerci
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Alefantis
authors.forename: Alexander
authors.initials: A
authors.lastname: Walz
authors.forename: Marian
authors.initials: M
authors.lastname: Quan
authors.forename: Ryan
authors.initials: R
authors.lastname: Chafin
authors.forename: Paul
authors.initials: P
authors.lastname: Grewal
authors.forename: Cesar V
authors.initials: CV
authors.lastname: Mujer
authors.forename: Rodolfo A
authors.initials: RA
authors.lastname: Ugalde
authors.forename: Vito G
authors.initials: VG
authors.lastname: DelVecchio
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001428:Bacterial Vaccines / Q000276:immunology*; D015153:Blotting, Western; D002003:Brucella abortus / Q000276:immunology / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000276:immunology; D020543:Proteome
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D008565:Membrane Proteins; D020543:Proteome