title: Injectable calcium phosphate bone cement provides favorable space and a scaffold for periodontal regeneration in dogs.
abstract: BACKGROUND
  An earlier study showed that an injectable calcium phosphate cement (CPC) served as a stable scaffold for bone formation and promoted histocompatible healing of periodontal tissue in dogs. In this study, we evaluated the influence of CPC on regeneration of periodontal defects with experimental periodontitis in dogs.
  
  
  METHODS
  Experimental periodontitis was induced by placing stainless-steel mesh on the mesial side of maxillary canines in six adult, healthy beagle dogs. Subsequently, intrabony defects were resized so as to be standard, and CPC was injected in the experimental bone defects. Non-grafted defects on the contralateral side served as controls. Twelve weeks after surgery, the animals were sacrificed and histologic specimens were prepared. Periodontal tissue healing was evaluated histologically and histometrically.
  
  
  RESULTS
  Healing of periodontal tissues, in terms of bone and cementum formation, was consistently observed in the CPC-applied sites. CPC was partly replaced by new bone. New cementum and periodontal ligament-like tissue were observed between CPC and the root surface. New bone (P <0.05), new cementum (P <0.01), and new connective tissue attachment and adhesion (P <0.05) were significantly enhanced in the experimental sites.
  
  
  CONCLUSIONS
  Calcium phosphate cement provides stable wound healing and enhanced periodontal regeneration in periodontal defects in dogs with experimental periodontitis.
authors.affiliation: Periodontology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. chiehayashi.peri@tmd.ac.jp
authors.forename: Chie
authors.initials: C
authors.lastname: Hayashi
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Kinoshita
authors.forename: Shigeru
authors.initials: S
authors.lastname: Oda
authors.forename: Koji
authors.initials: K
authors.lastname: Mizutani
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Shirakata
authors.forename: Isao
authors.initials: I
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D008297:Male; D008437:Maxilla / Q000601:surgery*; D010519:Periodontium / Q000187:drug effects
chemical_list: D001843:Bone Cements; D018786:Bone Substitutes; D002130:Calcium Phosphates; C020243:calcium phosphate