Trypanosoma cruzi: Oxidative stress induces arginine kinase expression.
Trypanosoma cruzi arginine kinase is a key enzyme in cell energy management and is also involved in pH and nutritional stress response mechanisms. T. cruzi epimastigotes treated with hydrogen peroxide presented a time-dependent increase in arginine kinase expression, up to 10-fold, when compared with untreated parasites. Among other oxidative stress-generating compounds tested, only nifurtimox produced more than 2-fold increase in arginine kinase expression. Moreover, parasites overexpressing arginine kinase showed significantly increased survival capability during hydrogen peroxide exposure. These findings suggest the participation of arginine kinase in oxidative stress response systems.
Miranda
Mariana R
MR
Laboratorio de Biología Molecular de Trypanosoma cruzi, Departamento de Sustancias Vasoactivas, Instituto de Investigaciones Médicas Alfredo Lanari, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Argentina.
Canepa
Gaspar E
GE
Bouvier
Leon A
LA
Pereira
Claudio A
CA
D000818:Animals; D001122:Arginine Kinase / Q000096:biosynthesis*; D015153:Blotting, Western; D004790:Enzyme Induction / Q000187:drug effects / Q000502:physiology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D051379:Mice; D008807:Mice, Inbred BALB C; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000032:analysis; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism
D016877:Oxidants; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D006861:Hydrogen Peroxide; D001122:Arginine Kinase