title: Core transcriptional regulatory circuitry in human hepatocytes.
abstract: We mapped the transcriptional regulatory circuitry for six master regulators in human hepatocytes using chromatin immunoprecipitation and high-resolution promoter microarrays. The results show that these regulators form a highly interconnected core circuitry, and reveal the local regulatory network motifs created by regulator-gene interactions. Autoregulation was a prominent theme among these regulators. We found that hepatocyte master regulators tend to bind promoter regions combinatorially and that the number of transcription factors bound to a promoter corresponds with observed gene expression. Our studies reveal portions of the core circuitry of human hepatocytes.
authors.affiliation: Young Laboratory, Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
authors.forename: Duncan T
authors.initials: DT
authors.lastname: Odom
authors.forename: Robin D
authors.initials: RD
authors.lastname: Dowell
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Jacobsen
authors.forename: Lena
authors.initials: L
authors.lastname: Nekludova
authors.forename: P Alexander
authors.initials: PA
authors.lastname: Rolfe
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Danford
authors.forename: David K
authors.initials: DK
authors.lastname: Gifford
authors.forename: Ernest
authors.initials: E
authors.lastname: Fraenkel
authors.forename: Graeme I
authors.initials: GI
authors.lastname: Bell
authors.forename: Richard A
authors.initials: RA
authors.lastname: Young
mesh_terms: D005786:Gene Expression Regulation; D022781:Hepatocytes; D006706:Homeostasis; D006801:Humans; D011401:Promoter Regions, Genetic; D040081:Protein Array Analysis; D014157:Transcription Factors; D014158:Transcription, Genetic
chemical_list: D014157:Transcription Factors