title: On-column sample enrichment for the high-sensitivity sheath-flow CE-MS analysis of peptides.
abstract: A sample enrichment technique to increase sensitivity in capillary electrophoresis-mass spectrometry (CE-MS) is described. Peptides or glycopeptides are retained and concentrated on a short (3-5-mm) reversed-phase (C18) packed-bed situated in the fused-silica separation capillary and are subsequently released for electrophoretic separation by injection of an organic elutant. The concentration limits of detection are in the high picomolar range with a sheath-flow CE-MS interface.
authors.affiliation: Laboratory of Protein Biochemistry and Protein Engineering, Ghent University, K.L. Ledeganckstraat 35, 9000 Gent, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Sandra
authors.forename: F
authors.initials: F
authors.lastname: Lynen
authors.forename: B
authors.initials: B
authors.lastname: Devreese
authors.forename: J
authors.initials: J
authors.lastname: Van Beeumen
authors.forename: P
authors.initials: P
authors.lastname: Sandra
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D010455:Peptides