[Dystrophin expression in mdx mice after bone marrow stem cells transplantation].
OBJECTIVE
To investigate the dynamic changes of dystrophin expression in mdx mice after bone marrow stem cells transplantation.


METHODS
The bone marrow stem cells of C57 BL/6 mice (aged 6 to 8 weeks) were injected intravenously into the mdx mice (aged 7 to 9 weeks), which were preconditioned with 7Gy gamma ray. The amount of dystrophin;expression in gastrocnemius was detected by immunofluorescence, reverse transcription-polymerase chain reaction and Western blot at week 5, 8, 12 and 16 after transplantation.


RESULTS
At week 5 after bone marrow stem cells transplantation, the dystrophin expression detected in mdx mice were very low; however, its expression increased along with time. At week 16 week, about 12% muscle cells of all transplanted mice expressed dystrophin. There were less centrally placed myonuclei than the control mdx mice, whereas peripheral myonuclei increased.


CONCLUSIONS
After having been injected into mdx mice, the allogenic bone marrow stem cells have a trend to reach the injured muscle tissues and differentiate to fibers that can express dystrophin and the expression increased with time. The bone marrow stem cells participates in the repair and regeneration of the injured tissues permanently and constantly.
Feng
Shan-wei
SW
Department of Neurology, the First Affiliated Hospital, SUN Yat-sen University, Guangzhou 510080, China.
Zhang
Cheng
C
Yao
Xiao-li
XL
Yu
Mei-juan
MJ
Li
Jing-lun
JL
Chen
Song-lin
SL
Liu
Tai-yun
TY
Lu
Xi-lin
XL
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D004195:Disease Models, Animal; D016189:Dystrophin / Q000096:biosynthesis*; D018380:Hematopoietic Stem Cell Transplantation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D020388:Muscular Dystrophy, Duchenne / Q000378:metabolism / Q000601:surgery*; D014184:Transplantation, Homologous
D016189:Dystrophin