[Effect of Herba Epimedii flavone on the osteoblasts metabolism in vitro].
OBJECTIVE
To explore the effect of Herba Epimedii flavone (HEF) on the osteoblast metabolism in vitro.


METHODS
Osteoblast were obtained from new born rat calvaria by digestive enzymes. MTF, PNPP and RT-PCR were used to observe the proliferation, activity of ALP and mRNA expression of OPG and RANKL of cultured osteoblasts in vitro.


RESULTS
It was found that HEF had the effect on stimulating cell proliferation, activity of ALP and the mRNA expression of OPG of cultured osteoblasts (P < 0.01, P < 0.05).


CONCLUSIONS
HEF can promote the proliferation, the differentiation and the expression of OPG mRNA of the osteoblasts cultured in vitro.
Liu
Yi-Heng
YH
Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. liuyiheng-xjtu@sohu.com
Zhang
Hai-Ying
HY
Zang
Hong-Min
HM
Cheng
Jun-Chang
JC
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D034181:Epimedium / Q000737:chemistry; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D053244:Osteoprotegerin; D010946:Plants, Medicinal / Q000737:chemistry; D053245:RANK Ligand; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis* / Q000235:genetics
D002352:Carrier Proteins; D047309:Flavones; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506032:Tnfrsf11b protein, rat; D000469:Alkaline Phosphatase