title: Some properties of beta-fructofuranosidases partially purified from Phaseolus vulgaris and Solanum tuberosum.
abstract: 1. Soluble beta-fructofuranosidases were purified 16-fold from French-bean-pod extracts and 35-fold from potato-tuber extracts. 2. The two enzymes had similar overall properties but differed from each other quantitatively in lability and reaction kinetics. 3. A non-diffusible inhibitor of beta-fructofuranosidase was present in the potato-tuber extracts but was absent from the bean extracts. This non-competitive inhibitor was acid-labile. 4. The possible roles of imidazole, carboxyl and thiol groups in beta-fructofuranosidase action are discussed, and the properties of the bean and potato enzymes are compared with published data for yeast, mould and grape-berry enzymes.
authors.affiliation: Department of Biochemistry, University of Birmingham, Edgbaston, Birmingham, 15.
authors.forename: G M
authors.initials: GM
authors.lastname: Frost
authors.forename: R N
authors.initials: RN
authors.lastname: Greenshields
authors.forename: F W
authors.initials: FW
authors.lastname: Teale