Costertonia aggregata gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from a mature biofilm.
A marine bacterium, strain KOPRI 13342(T), was isolated from a mature marine biofilm, including various marine algae, covering a rock-bed of the East Sea, Korea (also known as the Sea of Japan). Colonies of the isolate were orange-coloured on marine agar 2216. The isolate showed relatively high 16S rRNA gene sequence similarities to members of the genera Maribacter (91.2-92.4 % similarity), Zobellia (90.7-91.5 %) and Muricauda (90.7-91.4 %). Phylogenetic analysis based on the nearly complete 16S rRNA gene sequence revealed that the isolate formed a phyletic lineage with members of the genus Muricauda. Cells were aerobic, motile, Gram-negative rods and they produced non-diffusible carotenoid pigments. Optimal growth was observed at pH 7.5-8.0 and 26-32 degrees C and required the presence of 3 % (w/v) sea salt. The strain required Ca(2+) and K(+) ions in addition to NaCl for growth. The dominant fatty acids were i-15 : 0, i-15 : 1omega10, 15 : 0 and 16 : 1omega9. The major respiratory quinone was MK-6. The DNA G+C content was 35.8 mol%. On the basis of this polyphasic taxonomic evidence, strain KOPRI 13342(T) should be classified as a representative of a novel species in a new genus in the family Flavobacteriaceae; the name Costertonia aggregata gen. nov., sp. nov. is proposed. The type strain of Costertonia aggregata is KOPRI 13342(T) (=KCCM 42265(T) = JCM 13411(T)).
Kwon
Kae Kyoung
KK
Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, Ansan PO Box 29, 425-600, Republic of Korea.
Lee
Yoo Kyung
YK
Polar BioCenter, Korea Polar Research Institute, Korea Ocean Research and Development Institute, Ansan PO Box 29, 425-600, Republic of Korea.
Lee
Hong Kum
HK
Polar BioCenter, Korea Polar Research Institute, Korea Ocean Research and Development Institute, Ansan PO Box 29, 425-600, Republic of Korea.
D018441:Biofilms / Q000145:classification*; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny