title: Evaluation of relative fertility of cryopreserved goat sperm.
abstract: This study was designed to compare differences in the in vivo fertility of cryopreserved goat semen assessed by heterospermic insemination with differences in in vitro analyses. Five groups of does were inseminated with mixed frozen-thawed semen from different pairs of bucks. The percentage of offspring sired by each buck in the pair was compared with the relative ability of spermatozoa from that frozen-thawed ejaculate to penetrate zona-free hamster ova, relative post-thaw acrosomal integrity, ability to undergo an acrosome reaction during in vitro capacitation, and assessments of sperm motility. In 4 of the 5 different insemination pairs, the ratio of offspring born was other than 1:1. Acrosomal integrity, ability of spermatozoa to undergo an acrosome reaction, and parameters of sperm motility were not correlated with differences in relative fertility in this experiment using ejaculates from fertile bucks. The ability of spermatozoa to fuse with the oocyte plasma membrane was highly correlated with relative in vivo fertility (R2=0.78, P=0.04). This suggests that fusion with the oocyte plasma membrane is an event in the fertilization process in which significant variation exists among fertile bucks. Assessment of ability of spermatozoa to fuse with zona-free hamster ova may contribute to analysis of post-thaw fertility of frozen-thawed buck semen.
authors.affiliation: Department of Animal Science, University of California, Davis, CA 95616, USA.
authors.forename: T
authors.initials: T
authors.lastname: Berger
authors.forename: E Z
authors.initials: EZ
authors.lastname: Drobnis
authors.forename: L
authors.initials: L
authors.lastname: Foley
authors.forename: J K
authors.initials: JK
authors.lastname: Metzler
authors.forename: M
authors.initials: M
authors.lastname: Horton