Polysomes are associated with microtubules in fertilized eggs of Chinese pine (Pinus tabulaeformis).
Transmission electron microscopy of immunogold-labeled Chinese pine egg cells before and after fertilization revealed that polysomes are associated with microtubules (MTs) from fertilization to the 2-nucleate embryo stage. Ribosome aggregates of various size and shape were randomly distributed in the cytoplasm of the eggs before fertilization. Single MTs or clusters were observed to be free of polysomes at this stage. Upon fertilization, all polysomes were attached to MTs, and this association persisted until the formation of the polarized embryo. Thereafter, the polysomes spread into the cytoplasm and no polysome-MT association was observed in the embryo. Some of the polysomes were attached to one end of the MTs, while others appeared to form contacts along their entire length. No polysome-microfilament association was observed at any stage of the development. The polysome-MT association may provide a mechanism for MT-dependent mRNA localization in early embryo development of this plant.
Han
Yuzhen
Y
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Science, China Agricultural University, Beijing.
Yu
Jingjuan
J
Guo
Fengli
F
Watkins
Simon C
SC
D016764:Cell Polarity; D005306:Fertilization; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D028223:Pinus / Q000166:cytology* / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D011132:Polyribosomes / Q000378:metabolism* / Q000648:ultrastructure; D012639:Seeds / Q000378:metabolism; D015053:Zygote / Q000378:metabolism* / Q000648:ultrastructure