title: Effect of in vitro selenium supplementation on bovine sperm motility.
abstract: The effects of selenium (Se) on motility of bovine spermatozoa were evaluated in two experiments. In Experiment 1, increasing doses of Se alone or with vitamin E were found to increase motility in Se concentrations up to 1 mug/ml. However, Se alone had a greater effect than did Se with vitamin E. The sperm motility response to Se concentrations from background to 1 mug/ml on motility best fit a quadratic equation with a maximum at about .65 mug Se/ml. In Experiment 2, the effect of Se addition before and after freezing was evaluated. This experiment demonstrated that treatment of semen with Se before freezing significantly increased the post-thaw motility as compared with portions of the same ejaculate which were not treated. Treatment after thawing produced motilities which were intermediate and not significantly different from either pre-freezing supplementation or no supplementation at all. These data indicate that some semen exhibits a greater percentage of motile sperm when Se is added prior to freezing, however, no information is yet available on the fertility of Se-treated semen.
authors.affiliation: Department of Animal Science Ohio Agricultural Research and Development Center Wooster, Ohio 44691 USA; Department of Dairy Science Ohio Agricultural Research and Development Center Wooster, Ohio 44691 USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Siegel
authors.forename: F A
authors.initials: FA
authors.lastname: Murray
authors.forename: W E
authors.initials: WE
authors.lastname: Julien
authors.forename: A L
authors.initials: AL
authors.lastname: Moxon
authors.forename: H R
authors.initials: HR
authors.lastname: Conrad