[Screening the hALR-interacting protein from the cDNA library of hepatocarcinoma cells and studying its biological functions].
OBJECTIVE
To screen the hALR-interacting protein by phage-displayed technique and identify its biological activities.


METHODS
The specific phage clones that interacted with target protein hALR from a cDNA library of hepatocarcinoma cells were selected using the T7 phage-displayed technique. The acquired cDNA inserts were sequenced and analyzed by bioinformatic tools. The biological activities of the phage-displayed peptide affecting QGY hepatocarcinoma cells were studied using 3H-TdR method.


RESULTS
The cDNA inserts with 212 bp were acquired after 4 rounds of biopanning. They showed 100% homology with citron kinase. The phage-displayed peptide and the peptide combined with hALR affected QGY cells proliferation.


CONCLUSIONS
hALR-interacting peptide can be specifically screened by phage-displayed technique. Citron kinase that interacted with hALR potentially plays an important role in the proliferation of hepatocarcinoma cells.
Deng
Jian-chuan
JC
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Zhang
Lin
L
Sun
Hang
H
Tang
Lin
L
Wang
Na
N
Liu
Qi
Q
D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D015723:Gene Library; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008115:Liver Regeneration; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D050862:Oxidoreductases Acting on Sulfur Group Donors
D019151:Peptide Library; D010455:Peptides; D011506:Proteins; C088825:GFER protein, rat; D050862:Oxidoreductases Acting on Sulfur Group Donors