Immunophenotyping of surface antigens in acute myeloid leukemia by flow cytometry after red blood cell lysis.
Immunophenotyping of acute leukemia using flow cytometry after density gradient separation (dg-sep) of mononuclear cells is the international gold standard. But destroying red cells by whole blood lysis (wb-lysis) after direct staining has found a broad usage. Both methods revealed congruent results in phenotyping of 26 cases of acute myeloid leukemias by testing CD4, CD7, CD11b, CD11c, CD13, CD14, CD15, CD33, CD34, CD65s, Glycophorin A and HLA-DR antigens. Mean values of these tested antigens were very similar. Only low antigen expression of CD2 was missed in the phenotype of two AML cases by the wb-lysis (23 and 29% positive cells by the dg-sep).
Schwonzen
Martin
M
St. Walburga-Hospital, D-59870 Meschede, Germany. schwonzen@freenet.de
Diehl
Volker
V
Dellanna
Mario
M
Staib
Peter
P
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D006021:Glycophorins / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D016130:Immunophenotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008875:Middle Aged
D015703:Antigens, CD; D000954:Antigens, Surface; D006021:Glycophorins; D006684:HLA-DR Antigens