title: The human muscle proteome in aging.
abstract: The aim of the present study was to assess age-dependent changes of proteins in the vastus lateralis muscle of physically active elderly and young subjects by a combination of two-dimensional difference gel electrophoresis, SDS-PAGE and ESI-MS/MS. The differences observed in the elderly group included down-regulation of regulatory myosin light chains, particularly the phosphorylated isoforms, a higher proportion of myosin heavy chain isoforms 1 and 2A, and enhanced oxidative and reduced glycolytic capacity.
authors.affiliation: Institute of Molecular Bioimaging and Physiology, CNR, Segrate (MI), Italy. cecilia.gelfi@ibfm.cnr.it
authors.forename: Cecilia
authors.initials: C
authors.lastname: Gelfi
authors.forename: Agnese
authors.initials: A
authors.lastname: Vigano
authors.forename: Marilena
authors.initials: M
authors.lastname: Ripamonti
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pontoglio
authors.forename: Shajna
authors.initials: S
authors.lastname: Begum
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Pellegrino
authors.forename: Bruno
authors.initials: B
authors.lastname: Grassi
authors.forename: Roberto
authors.initials: R
authors.lastname: Bottinelli
authors.forename: Robin
authors.initials: R
authors.lastname: Wait
authors.forename: Paolo
authors.initials: P
authors.lastname: Cerretelli
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008969:Molecular Sequence Data; D009043:Motor Activity; D018482:Muscle, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism; D010766:Phosphorylation; D020543:Proteome / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D018994:Myosin Light Chains; D020543:Proteome; D018995:Myosin Heavy Chains