The protective effects of Phyllanthus emblica Linn. extract on ethanol induced rat hepatic injury.
This study was undertaken to investigate the protective effects of Phyllanthus emblica Linn. (PE) extract on ethanol induced rat hepatic injury. PE (0.5 and 1 mg/ml) increased cell viability of rat primary cultured hepatocytes being treated with ethanol (96 microl/m) by increasing % MTT and decreasing the release of transaminase. Hepatotoxic markers studied in rats included serum transaminases (AST and ALT), serum triglyceride (STG), hepatic triglyceride (HTG), TNF-alpha and IL-1beta together with histopathological examination. Pretreatment of rats with PE at oral dose of 25, 50 and 75 mg/kg or SL (silymarin, a reference hepatoprotective agent) at 5 mg/kg, 4 h before ethanol, lowered the ethanol induced levels of AST, ALT and IL-1beta. The 75 mg/kg PE dose gave the best result similar to SL. Treatment of rats with PE (75 mg/kg/day) or SL (5 mg/kg/day) for 7 days after 21 days with ethanol (4 g/kg/day, p.o.) enhanced liver cell recovery by bringing the levels of AST, ALT, IL-1beta back to normal. Histopathological studies confirmed the beneficial roles of PE and SL against ethanol induced liver injury in rats.
Pramyothin
Pornpen
P
Pharmacological Action of Natural Products Research Unit, Department of Pharmacology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand. ppornpen@chula.ac.th
Samosorn
Patcharavadee
P
Poungshompoo
Somlak
S
Chaichantipyuth
Chaiyo
C
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D005638:Fruit; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D053583:Interleukin-1beta / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D029842:Phyllanthus emblica; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012838:Silymarin / Q000494:pharmacology; D013997:Time Factors; D014280:Triglycerides / Q000097:blood / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D053583:Interleukin-1beta; D010936:Plant Extracts; D020011:Protective Agents; D012838:Silymarin; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase