The effect of down-regulation of mitochondrial RNA-binding proteins MRP1 and MRP2 on respiratory complexes in procyclic Trypanosoma brucei.
MRP1 and MRP2 are multifunctional mitochondrial RNA-binding proteins with a regulatory role in RNA editing and putative role(s) in RNA processing in Trypanosoma brucei. Silencing of MRP1 and/or MRP2 by RNA interference affected the assembly and functionality of respiratory complexes. The absence of several subunits of complexes I, III and IV resulted in their disintegration and subsequent decrease of specific activities and also caused a significant decrease of membrane potential. The overall respiration in the interfered cells decreased by only about 20%, since the trypanosome alternative oxidase effectively replaced the missing cytochromes and became the principal terminal oxidase.
Zíková
Alena
A
Institute of Parasitology, Czech Academy of Sciences, and Faculty of Biology, University of South Bohemia, Branisovská 31, 37005 Ceské Budejovice, Czech Republic.
Horáková
Eva
E
Jirků
Milan
M
Dunajcíková
Petra
P
Lukes
Julius
J
D000818:Animals; D015536:Down-Regulation; D004579:Electron Transport; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000235:genetics; D017393:RNA Editing; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000378:metabolism*
D045222:Electron Transport Chain Complex Proteins; D024101:Mitochondrial Proteins; D016601:RNA-Binding Proteins; D003576:Electron Transport Complex IV; D006180:Proton-Translocating ATPases