title: [Determination of tetrodotoxin in puffer-fish tissues, and in serum and urine of intoxicated humans by liquid chromatography with tandem mass spectrometry].
abstract: A simple and rapid method was developed for the analysis of tetrodotoxin in puffer-fish tissues, and in serum and urine of humans poisoned after consuming puffer-fish, by means of high-performance liquid chromatography with tandem mass spectrometry (LC/MS/MS). Tetrodotoxin was extracted with 2% acetic acid. The extracted solution from puffer-fish tissues was diluted with water, and the extracted solution from human serum and urine was cleaned up by LC/MS/MS with a methacrylate-styrenedivinylbenzene cartridge. The LC separation was performed on a C18 column (50 mm x 2.1 mm i.d.) using 10 mmol/L IPCC-MS7-methanol (65 : 35) as the mobile phase at a flow rate of 0.2 mL/min. The mass spectral acquisition was done in the positive ion mode by applying selected reaction monitoring (SRM). The recoveries of tetrodotoxin were 79-90% from puffer-fish tissues fortified at 0.1 microg/g and 1 microg/g, and 93-101% from human serum and urine fortified at 0.5 ng/mL and 5 ng/mL. The detection limits of tetrodotoxin were 0.01 microg/g in puffer-fish tissues and 0.1 ng/mL in human serum and urine. Thirty samples of puffer-fish from wholesale markets, and 7 serum and 5 urine samples of humans poisoned after consuming puffer-fish were analyzed by this method. Tetrodotoxin was detected in all puffer-fish tissues, and all serum and urine samples at the levels of 0.04-140 microg/g, 0.9-1.8 ng/mL and 15-150 ng/mL, respectively.
authors.affiliation: Fukuoka City Institute for Hygiene and the Environment, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Akaki
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Hatano
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002853:Chromatography, Liquid; D005260:Female; D005517:Foodborne Diseases / Q000097:blood / Q000378:metabolism* / Q000652:urine; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D023721:Tetraodontiformes / Q000378:metabolism*; D013779:Tetrodotoxin / Q000032:analysis* / Q000097:blood / Q000506:poisoning* / Q000652:urine
chemical_list: D013779:Tetrodotoxin