Evaluation of the Duopath Legionella lateral flow assay for identification of Legionella pneumophila and Legionella species culture isolates.
Duopath Legionella (Merck KGaA, Darmstadt, Germany) is a new immunochromatographic assay for the simultaneous identification of cultured L. pneumophila and Legionella species other than L. pneumophila. In tests of 89 L. pneumophila strains and 87 Legionella strains other than L. pneumophila representing 41 different species, Duopath and a widely used latex agglutination assay detected L. pneumophila with 100% and 98% accuracy, respectively, whereas the percentages differed significantly for other Legionella spp. (93% versus 37% [P < 0.001]). Since many countries' regulations require the identification of Legionella spp. in water and environmental samples, the use of Duopath Legionella to comply with those regulations could contribute to significantly fewer false-negative results.
Helbig
Jürgen Herbert
JH
Institute of Medical Microbiology and Hygiene, Medical Faculty of the Technical University Dresden, Fetscherstr. 74, D-01307 Dresden, Germany. j.helbig@mailbox.tu-dresden.de.
Lück
Paul Christian
PC
Kunz
Britta
B
Bubert
Andreas
A
D002845:Chromatography / Q000379:methods; D005188:False Negative Reactions; D007841:Latex Fixation Tests; D007875:Legionella / Q000145:classification / Q000302:isolation & purification*; D016952:Legionella pneumophila / Q000145:classification / Q000302:isolation & purification*; D011549:Pseudomonas / Q000145:classification / Q000302:isolation & purification; D015203:Reproducibility of Results