title: The effect of vitamin A deficiency and dietary alpha-tocopherol on the stability of rat-liver lysosomes.
abstract: 1. Vitamin A-deficient rats and pair-fed controls were maintained on either normal or raised amounts of dietary alpha-tocopherol. 2. Their livers were fractionated and ;free' and ;total' lysosomal phosphatase were determined in the various fractions. The rate of release of this enzyme was determined in the mitochondria-lysosome-rich fraction during incubation at pH5 and 37 degrees . 3. The deficient livers showed increased enzymic activity. 4. Prolonged incubation caused more rapid enzyme release from the mitochondria-lysosome-rich fraction of the vitamin A-deficient rats receiving the normal amount of dietary alpha-tocopherol than from the equivalent fraction of their pair-fed controls receiving vitamin A. Raised dietary alpha-tocopherol reversed this phenomenon.
authors.affiliation: Columbia University Institute of Nutrition Sciences, New York, N.Y., U.S.A.
authors.forename: O A
authors.initials: OA
authors.lastname: Roels
authors.forename: M
authors.initials: M
authors.lastname: Trout
authors.forename: A
authors.initials: A
authors.lastname: Guha