title: [Effect of 2, 5-hexanedione on calcium homeostasis of motor neuron].
abstract: OBJECTIVE
  To explore the mechanism of cytotoxic effect of 2, 5-hexanedione (2, 5-HD) on motor neuron.
  
  
  METHODS
  Vsc4.1 (a cell line from motor neuron) was incubated with a series concentration of 2, 5-HD. The cell viability, Ca(2+) Mg(2+) ATPase and Na(+)K(+) ATPase were detected. Laser scanning confocal microscope (LSCM) was used for detecting intracellular calcium level. The average calcium level in VSC4.1 was measured by flow cytometry.
  
  
  RESULTS
  The cell viability was decreased when Vsc4.1 cells were treated with 2, 5-HD at the dosage of 2.5, 5.0, 7.5, 10, 15 and 20 mmol/L for 24 hours. Compared with the control group the activity of Ca(2+) Mg(2+) ATPase was decreased to 70.02%, 77.44% and 47.47% respectively; the activity of Na(+)K(+) ATPase was decreased to 82.07%, 72.45% and 50.71%. The difference was significant. Intracellular free calcium of VSC4.1 cell was increased rapidly within 10 s and then recovered within 40 seconds when it was exposed to 33.5 mmol/L 2, 5-HD. An increase in intracellular calcium was observed when the VSC4.1 was treated with 33.5 mmol/L 2, 5-HD. The peak of intracellular calcium level occurred ten minutes later.
  
  
  CONCLUSIONS
  The disturbance of calcium homeostasis may be involved in the mechanisms of neurotoxicity of 2, 5-HD.
authors.affiliation: National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, PR China, 100050.
authors.forename: Yan-shu
authors.initials: YS
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Qing-jun
authors.initials: QJ
authors.lastname: Liu
authors.forename: Hua-wei
authors.initials: HW
authors.lastname: Duan
authors.forename: Feng-sheng
authors.initials: FS
authors.lastname: He
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Zheng
mesh_terms: D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006588:Hexanones / Q000633:toxicity*; D009046:Motor Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D006588:Hexanones; C011269:2,5-hexanedione; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium