title: Pseudomonas aeruginosa epidemic strain carrying bla(SPM) metallo-beta-lactamase detected in Rio de Janeiro, Brazil.
abstract: The present study was designed to characterize beta-lactamase genes and evaluate polymerase chain reaction (PCR) typing for multidrug-resistant Pseudomonas aeruginosa pulsed-field gel electrophoresis (PFGE) genotype A isolates from Rio de Janeiro, Brazil, collected between April 1999 and March 2000 and one additional isolate collected in June 2002. As reported previously, all of the genotype A isolates produced non-characterized metallo-beta-lactamase. These isolates (22) were screened for the bla(SPM) gene by PCR and dot-blotting. Isolates were typed by PCR fingerprinting with primers RAPD-1, 272, 208, 1290, ERIC-1 and ERIC-2. The bla(SPM) gene was detected in 18 (82%) of the 22 isolates. PCR fingerprinting gave results that correlated with PFGE, except with primer 1290. In Rio de Janeiro and other Brazilian states, nearly all SPM-producing P. aeruginosa isolates belong to a single PFGE type accounting for a large proportion of drug-resistant P. aeruginosa hospital infections. RAPD PCR fingerprinting may be a useful technique to screen for an epidemic multidrug-resistant strain in Brazil.
authors.affiliation: Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: F L P C
authors.initials: FL
authors.lastname: Pellegrino
authors.forename: N
authors.initials: N
authors.lastname: Casali
authors.forename: K R N
authors.initials: KR
authors.lastname: Dos Santos
authors.forename: S A
authors.initials: SA
authors.lastname: Nouér
authors.forename: E M D
authors.initials: EM
authors.lastname: Scheidegger
authors.forename: L W
authors.initials: LW
authors.lastname: Riley
authors.forename: B M
authors.initials: BM
authors.lastname: Moreira
mesh_terms: D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D001938:Brazil / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C476155:beta-lactamase SPM-1, Pseudomonas aeruginosa; D001618:beta-Lactamases