title: Influence of organochlorine pesticides on physiological potency of cultured granulosa cells from bovine preovulatory follicles.
abstract: In the present study the direct effect of Dichlorodiphenyltrichloroethane (DDT), Methoxychlor (MXC) and Hexachlorocyclohexane (gammaHCH) was investigated on the DNA synthesis (measured by [3H]thymidine incorporation); proliferation (determined by colorimetric MTT[3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide] assay); and steroidogenesis (estradiol 17beta, progesterone determination) of granulosa cells during in vitro culture. The cells were aspirated from preovulatory follicles in PMSG-treated heifers. They were cultivated in phenol red and serum (0.1% FCS) RPMI 1640 medium without or with the 3 pesticides in different concentrations. In summary, the results showed differential inhibition of parameters named above by these pesticides (gammaHCH < MXC < DDT) in comparison with vehicle-treated control. Progesterone synthesis was more inhibited than estradiol 17beta production by 3 chemicals, which resulted from the loss of viability of cells indicated by estimation of cell-shape-factor and viability of cells. Besides the inhibitory effect, there was a stimulatory effect of low concentrations of DDT and MXC on [3H]thymidine incorporation and proliferation.
authors.affiliation: Department of Reproductive Biology, Research Institute for Biology of Farm Animals, Dummerstorf, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Tiemann
authors.forename: R
authors.initials: R
authors.lastname: Pöhland
authors.forename: F
authors.initials: F
authors.lastname: Schneider