Real-time in vivo virtual histology of colorectal lesions when using the endocytoscopy system.
BACKGROUND
The histological findings of GI lesions are based on light-microscopic examination of H&E-stained thin-slice specimens. Recently, a concept of optical biopsy has been advocated. A study of the observation of colorectal lesions using endocytoscopy to obtain real-time histological images in vivo during endoscopy was performed.


METHODS
Prospective study.


OBJECTIVE
To evaluate the usefulness of optical biopsy of colorectal lesions with the endocytoscopy (E-C) system.


METHODS
The subjects were 113 consecutive patients who underwent a complete colonic examination, from April 2003 to March 2004, performed by a single colonoscopist.


METHODS
Digestive Disease Center of Showa University Northern Yokohama Hospital.


RESULTS
With E-C, it was possible to observe lesions at the cellular level and evaluate cellular atypia in addition to structural atypia in vivo. The correlation was statistically significant between the endocytoscopic diagnosis and the histological diagnosis.


CONCLUSIONS
The endocytoscope had to be touched to the target colonic glands.


CONCLUSIONS
It was possible to distinguish neoplastic from non-neoplastic lesions, and also possible to distinguish invasive cancer from adenoma. "Ultra-high" magnifying endoscopy, the E-C system, provides real-time histological images in vivo, which correspond well with those of H&E-stained microscopic images.
Sasajima
Keita
K
Digestive Disease Center, Department of Pathology, Showa University Northern Yokohama Hospital, Chigasaki-chuo 35-1, Tsuzuki-ku, Yokohama 224-8503, Japan.
Kudo
Shin-ei
SE
Inoue
Haruhiro
H
Takeuchi
Tsukasa
T
Kashida
Hiroshi
H
Hidaka
Eiji
E
Kawachi
Hiroshi
H
Sakashita
Masanori
M
Tanaka
Junichi
J
Shiokawa
Akira
A
D000236:Adenoma / Q000000981:diagnostic imaging / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology*; D016099:Endoscopy, Gastrointestinal / Q000379:methods*; D005260:Female; D006801:Humans; D008875:Middle Aged; D011446:Prospective Studies; D014463:Ultrasonography