Differential trafficking and desensitization of human ET(A) and ET(B) receptors expressed in HEK 293 cells.
Endothelin-1 (ET-1) is a vasoconstrictor peptide that acts on ET(A) and ET(B) receptors on smooth muscle cells (SMCs). Because vascular SMCs can express both receptors, it is difficult to study the localization and properties of each subtype. Therefore, we investigated the localization and function of ET(A) and ET(B) receptors transfected into HEK 293 cells. Immunocytochemistry was used to examine colocalization of ET receptors with the plasma membrane marker, pan cadherin. In cells transfected with ET(A) receptors, 83 +/- 2% of these receptors colocalized with pan cadherin. In ET(B) receptor-transfected cells, 54 +/- 2% of the receptor colocalized with pan cadherin. When ET(A) and ET(B) receptors were cotransfected, 97 +/- 1% of ET(B) receptors colocalized with ET(A) receptors and 84 +/- 2% of ET(B) receptors colocalized with pan cadherin. ET-1 and sarafotoxin 6c (S6c, ET(B) receptor agonist) increased [Ca2+]i in cells transfected with ET(A) or ET(B) receptors; 100 nM of ET-1 and S6c caused maximal responses. When stimulated with ET-1, ET(B) receptors desensitized faster (t(1/2) = 21 +/- 1 sec) than ET(A) receptors (t(1/2) = 48 +/- 1 sec). S6c-induced increases in [Ca2+]i desensitized in cells expressing ET(B) receptors only (t(1/2) = 17 +/- 1 s). Desensitization was eliminated in cells cotransfected with ET receptors. We conclude that ET(A) receptors localize to the cell membrane, whereas ET(B) receptors are in the membrane and intracellular compartments. Coexpressed ET receptors are in the membrane. ET(B) receptors desensitize faster than ET(A) receptors, but receptor coexpression eliminates desensitization. Finally, ET(A) and ET(B) receptors interact to change receptor trafficking which may modify ET receptor function in vascular SMCs coexpressing these receptors.
Dai
Xiaoling
X
Department of Pharmacology and Toxicology and Neuroscience Program, Life Science Building Room B308, Michigan State University, East Lansing, MI 48824, USA. daixiaol@msu.edu
Galligan
James J
JJ
D002118:Calcium / Q000032:analysis; D002451:Cell Compartmentation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003888:Desensitization, Immunologic; D019281:Dimerization; D019332:Endothelin-1 / Q000494:pharmacology; D000939:Epitopes; D006388:Hemagglutinins / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism*; D044022:Receptor, Endothelin A / Q000737:chemistry / Q000276:immunology* / Q000502:physiology*; D044023:Receptor, Endothelin B / Q000737:chemistry / Q000276:immunology* / Q000502:physiology*; D013997:Time Factors; D014162:Transfection; D014757:Viper Venoms / Q000494:pharmacology
D019332:Endothelin-1; D000939:Epitopes; D006388:Hemagglutinins; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D014757:Viper Venoms; C057354:sarafotoxins s6; D002118:Calcium