Genomic variation in the mitochondrially encoded cytochrome b (MT-CYB) and 16S rRNA (MT-RNR2) genes: characterization of eight endangered Pecoran species.
In an effort to develop species-specific identification markers, we examined genetic variants and molecular signatures within genes encoding mitochondrial cytochrome b and 16S rRNA in eight endangered Pecoran species endemic to the Indian peninsula. Our results revealed that the cytochrome b gene exhibited higher sequence diversity than the 16S rRNA gene, both between and within species. However, the 16S rRNA gene harboured a larger number of species-specific mutation sites compared with the cytochrome b gene, suggesting that it could be useful for species identification. Indeed, we successfully used 'forensically informative nucleotide sequencing' (FINS) analysis of the 16S rRNA gene to identify two previously unknown biological specimens.
Guha
S
S
Central Forensic Science Laboratory, Kolkata, India.
Goyal
S P
SP
Kashyap
V K
VK
D000818:Animals; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics*; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000032:analysis; D007194:India; D024101:Mitochondrial Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012418:Ruminants / Q000145:classification* / Q000235:genetics*; D013045:Species Specificity
D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins; D012336:RNA, Ribosomal, 16S; D045303:Cytochromes b