Bemisia tabaci transmission of specific Lettuce infectious yellows virus genotypes derived from in vitro synthesized transcript-inoculated protoplasts.
Bemisia tabaci transmission was demonstrated for virions derived from cloned infectious cDNAs of Lettuce infectious yellows virus (LIYV). RNA transcripts synthesized from the cDNA clone of LIYV RNA 1 and for clones of seven genotypes (pR6, p1-1a, p1-2b, p1-2f, p1-4h, p1-5b, and p1-5d) of LIYV RNA 2 produced virions or virion-like particles (VLPs) when inoculated to tobacco protoplasts. pR6, p1-1a, and p1-2f virions were transmissible to plants by B. tabaci and transmission frequencies ranged from 15 to 80%, depending on the virion concentration. In contrast, no transmission was observed for p1-5b VLPs even though their concentrations were comparable to that of transmissible virions. p1-5b VLPs also differed from transmissible virions by the absence of an intact CPm, and this correlated with an out-of-frame stop codon mutation in the CPm gene. This is the first report of the vector transmissibility of infectious cDNAs-derived virions for a virus in the genus Crinivirus.
Ng
James C K
JC
Department of Plant Pathology, 900 University Ave., University of California, Riverside, CA 92521, USA.
Falk
Bryce W
BW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D029383:Crinivirus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D005838:Genotype; D006430:Hemiptera / Q000821:virology*; D007303:Insect Vectors / Q000821:virology*; D018545:Lactuca / Q000821:virology*; D008969:Molecular Sequence Data; D011523:Protoplasts / Q000821:virology; D014026:Nicotiana / Q000821:virology; D014158:Transcription, Genetic; D014771:Virion / Q000145:classification / Q000235:genetics / Q000502:physiology