title: Relationship between fertility of bovine semen and in vitro induction of acrosome reactions by heparin.
abstract: This study was designed to relate nonreturn rates of bulls in a commercial artificial insemination programme to the in vitro induction of acrosome reactions by the glycosaminoglycan, heparin. Semen was collected from twelve 1 to 2 year-old Holstein bulls. Washed spermatozoa from 4 to 6 ejaculates were incubated for 6 hours with 10 mug/ml heparin. Acrosome reactions were determined by differential interference microscopy. The percentage increase in acrosome reaction in heparin-treated compared to control samples was significantly correlated to the 90-day nonreturn rate of the bulls (r = 0.86; P<0.001). A significant correlation was obtained between the fertility of bulls predicted on the basis of acrosome reaction induction and achieved 90-day nonreturn rate (r=0.81; P<0.01).
authors.affiliation: Hampshire Cattle Breeders Society Ltd., Beechen Lane, Lyndhurst, Hants., SO43 7NN, UK.
authors.forename: C H
authors.initials: CH
authors.lastname: Whitfield
authors.forename: T J
authors.initials: TJ
authors.lastname: Parkinson