title: Limitations of oviductal transfers in swine.
abstract: Eighty-six crossbred gilts were used in a series of experiments 1) to determine if eight-cell embryos enter the uterus before four-cell embryos do, and 2) to investigate the effects of transferring older, more developed embryos, either with or without intact zona pellucidae, to the oviducts of swine. Results of these experiments suggested that both four-and eight-cell embryos randomly enter the uterus on Days 3.5 to 4 (Day 0 = onset of estrus). Though there were no differences in survival rates or subsequent morphology of recovered embryos when transferred to the oviduct or uterus on Day 4, the transfer. of Day-6 embryos to the oviduct resulted in lower (P < 0.01) survival rates, and the recovered embryos were smaller (P < 0.01) than those introduced into the uterus. Survival rates were lower (P < 0.06) for zonae-free embryos transferred to the oviduct than for zonae-intact embryos; however, these differences were not evident when zonae-free embryos were transferred into the uterus. These experiments demonstrate that oviductal transfer procedures in swine are limited to zonae-intact, preblastocyst stage embryos.
authors.affiliation: Department of Animal Science The Ohio State University Columbus, OH 43210 USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Broermann
authors.forename: S
authors.initials: S
authors.lastname: Xie
authors.forename: K P
authors.initials: KP
authors.lastname: Nephew
authors.forename: W F
authors.initials: WF
authors.lastname: Pope