title: Characterization and isolation of L-to-D-amino-acid-residue isomerase from platypus venom.
abstract: Platypus venom contains an isomerase that reversibly interconverts the second amino-acid residue in some peptides between the L-form and the D-form. The enzyme acts on the natriuretic peptides OvCNPa and OvCNPb, and on the defensin-like peptides DLP-2 and DLP-4, but it does not act on DLP-1. While the isomerization of DLP-2 to DLP-4 is inhibited by the amino-peptidase inhibitor amastatin, it is not affected by the leucine amino-peptidase inhibitor bestatin. The enzyme, that is only present in minute quantities in an extract of the venom gland, is thermally stable up to 55 degrees C, and it was found by anion-exchange chromatography to be acidic. Isolation of the isomerase was carried out by combined ion-exchange chromatography and reverse-phase high performance liquid chromatography (HPLC).
authors.affiliation: School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia.
authors.forename: A M
authors.initials: AM
authors.lastname: Torres
authors.forename: M
authors.initials: M
authors.lastname: Tsampazi
authors.forename: E C
authors.initials: EC
authors.lastname: Kennett
authors.forename: K
authors.initials: K
authors.lastname: Belov
authors.forename: D P
authors.initials: DP
authors.lastname: Geraghty
authors.forename: P S
authors.initials: PS
authors.lastname: Bansal
authors.forename: P F
authors.initials: PF
authors.lastname: Alewood
authors.forename: P W
authors.initials: PW
authors.lastname: Kuchel
mesh_terms: D000591:Amino Acid Isomerases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D007536:Isomerism; D020098:Natriuretic Peptide, C-Type / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010987:Platypus / Q000378:metabolism; D011480:Protease Inhibitors / Q000737:chemistry; D014688:Venoms / Q000201:enzymology*
chemical_list: D010455:Peptides; D011480:Protease Inhibitors; D014688:Venoms; D020098:Natriuretic Peptide, C-Type; C016977:amastatin; D000591:Amino Acid Isomerases