A comparison of three methods of recovery of goat oocytes for in vitro maturation and feritilization.
Experiments were conducted to determine an efficient method for recovering a large number of usable oocytes and its effect on subsequent in vitro maturation, fertilization and development. Follicular oocytes were recovered from goat ovaries using 3 different methods: aspiration, puncturing and slicing. The average total number of oocytes recovered per ovary was significantly higher by the aspiration method (2.7+/-0.15) than by puncturing (2.2+/-0.13) or by slicing (2.4+/-0.12). However, significantly more good-quality usable oocytes enclosed with compact cumulus cells were obtained by slicing (0.9+/-0.06) than by aspiration (0.5+/-0.07) or by puncturing (0.5+0.06). Time required for processing each ovary by the slicing method was comparatively less (0.90 min) than that required for puncturing (1.83 min) or for aspiration (1.78 min). Usable oocytes recovered by all three methods were matured, fertilized and developed to the blastocyst stage in vitro. There were no significant differences in the subsequent percentages of oocytes maturing, being fertilized and developing in vitro among the 3 methods of recovering oocytes. In conclusion, the recovery of goat oocytes by the slicing method is simple and efficient compared with the aspiration and puncturing methods.
Pawshe
C H
CH
Embryo Biotechnology Laboratory National Institute of Immunology Aruna Asaf Ali Marg, New Delhi 110067 India.
Totey
S M
SM
Jain
S K
SK