Design considerations for high speed quantitative mass spectrometry with MALDI ionization.
A MALDI ion source on a triple quadrupole mass spectrometer constructed for the purpose of obtaining high speed quantitative measurements on drugs and other low molecular weight compounds is described. Particular attention is given to the ion generation and transport phenomena that affect analysis speed, throughput, and practical instrument robustness. In this regard parameters that affect desorption speed, beam spreading, ion flight times, sensitivity, signal-to-noise, ion fragmentation, sample carry-over, and instrument contamination are examined and experimental results are provided. MALDI and electrospray sensitivity is compared, to provide a practical frame of reference.
Corr
Jay J
JJ
MDS SCIEX, 71 Four Valley Drive, L4K 4V8, Concord, ON, Canada.
Kovarik
Peter
P
MDS SCIEX, 71 Four Valley Drive, L4K 4V8, Concord, ON, Canada.
Schneider
Bradley B
BB
MDS SCIEX, 71 Four Valley Drive, L4K 4V8, Concord, ON, Canada.
Hendrikse
Jan
J
MDS SCIEX, 71 Four Valley Drive, L4K 4V8, Concord, ON, Canada.
Loboda
Alexander
A
MDS SCIEX, 71 Four Valley Drive, L4K 4V8, Concord, ON, Canada.
Covey
Thomas R
TR
MDS SCIEX, 71 Four Valley Drive, L4K 4V8, Concord, ON, Canada. Tom.Covey@sciex.com.
D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007834:Lasers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods