Anti-inflammatory and analgesic activity of the topical preparation of Verbena officinalis L.
Verbena officinalis has traditionally been used in herbal medicine in Navarra, Spain, in the treatment of topical inflammation. Due to the anti-inflammatory activity of Verbena officinalis 50% methanolic extract in i.p. and topical administration, the effects of several formulations were prepared and studied using carrageenan-induced edema and formalin testing. Piroxicam gel and methyl salicylate ointment were studied as positive control for anti-inflammatory and analgesic activity, respectively. The edema inhibition of the preparations containing extract at the doses of 1-3% w/w were significantly different from the control group. The anti-inflammatory effect of VO-3% was similar to the effect of piroxicam gel 3 h after carrageenan injection. The analgesic activity of topical preparation with more than 2.5% w/w was observed in the early phase. This activity was observed in concentrations of more than 2% w/w in the late phase. The topical analgesic activity of the extract was less than the analgesic activity of methyl salicylate ointment.
Calvo
M I
MI
Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, University of Navarra, C/Irularrea s/n, 31008, Pamplona Spain. mcalvo@unav.es
D000287:Administration, Topical; D000700:Analgesics / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002351:Carrageenan; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control*; D005557:Formaldehyde; D008297:Male; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control*; D010147:Pain Measurement; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D032412:Verbena
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D005557:Formaldehyde; D002351:Carrageenan