title: Cytogenetic evaluation of in vitro matured bovine oocytes collected from ovaries of individual donors.
abstract: Oocytes were collected after slaughter by aspiration from pairs of ovaries of individual donors. A total of 656 oocytes was selected for IVM from 74 pairs of ovaries (8.9 oocytes per pair, ranging between 1 and 25). The oocytes were matured in droplets of maturation medium (TCM-199 medium supplemented with 20% estrous cow serum (ECS), 50 microg/ml gentamycin, 10 microg/ml FSH, 1 microg/ml estradiol-17beta). Cytogenetic analysis of 348 oocytes showed 79 at the first metaphase (MI; 22.7%, 79 348 ), 11 at the first telophase (TI; 3.2%, 11/348 ), and 258 at the second metaphase (MII; 74.1% 258/348 ). Significant differences (P < 0.01) were shown among the donors regarding the number of oocytes selected for IVM and the number of oocytes matured for IVF.
authors.affiliation: Department of Genetics and Animal Breeding, Agricultural University of Poznań, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Sosnowski
authors.forename: M
authors.initials: M
authors.lastname: Switoński
authors.forename: D
authors.initials: D
authors.lastname: Lechniak
authors.forename: K
authors.initials: K
authors.lastname: Moliński