title: Genetic analysis of angiotensinogen gene polymorphisms (M235T and T174M) in Romanian patients with essential arterial hypertension.
abstract: BACKGROUND
  Several studies suggested the role of the genes of the rennin-angiotensin system in the pathogenesis of essential arterial hypertension.
  
  
  METHODS
  We investigated 100 consecutive hypertensive patients (42 males and 58 females; mean +/- SD age, 55.14 +/- 9.49 years) for M235T and T174M polymorphisms. Seventy six normotensive patients (28 males and 48 females; mean +/- SD age, 54.94 +/- 9.56 years) formed the control group.
  
  
  METHODS
  M235T and T174M polymorphisms were analyzed using PCR-RFLP methods.
  
  
  RESULTS
  The carrier frequency of M235T polymorphism in the AGT gene was 73% in hypertensive patients and 34.21% in normotensive patients (OR 5.2, 95%CI [2.72-9.93], p<0.01, chi2 test). The carrier frequency of T174M polymorphism in the AGT gene was 42% in hypertensive patients and 11.84% in normotensive patients (OR 5.39, 95%CI [2.41-12.01], p<0.01, chi2 test).
  
  
  CONCLUSIONS
  Essential arterial hypertension is associated with polymorphisms in the AGT gene, M235T and T174M.
authors.affiliation: Department of Medical Biochemistry, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. Lprocopciuc@personal.ro
authors.forename: Lucia Maria
authors.initials: LM
authors.lastname: Procopciuc
authors.forename: Dana
authors.initials: D
authors.lastname: Pop
authors.forename: D
authors.initials: D
authors.lastname: Zdrenghea
authors.forename: Gh
authors.initials: G
authors.lastname: Jebeleanu
mesh_terms: D000328:Adult; D000808:Angiotensinogen / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012016:Reference Values; D012383:Romania
chemical_list: D000808:Angiotensinogen