[Relationship between polymorphisms of vitamin D receptor gene and familial aggregation of HBsAg carriers].
OBJECTIVE
To determine whether -Taq I T/C and -Fok I C/T polymorphisms of vitamin D receptor (VDR) gene are associated with the familial aggregation of hepatitis B virus (HBV) infection.


METHODS
Based on a population-based case-control family design, 288 family members from 27 case families and 230 family members from 27 control families were recruited. VDR gene polymorphisms were analyzed. VDR-Taq I T/C and VDR-Fok I C/T polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism.


RESULTS
The frequency of VDR-Taq I TT genotype in the case families was significantly higher than that in the control families (P < 0.05) , however, the frequency of VDR-Fok I CC genotype in the case families was significantly higher than that in the control families (P < 0.05). The frequency of family members carriying Taq I T-Fok I C haplotype in the case families was significantly higher than that in the control families (OR = 1.67, P < 0.05), however, the frequency of family members carrying Taq I C-Fok I T haplotype in the case families was significantly lower than that in the control families (OR = 0. 24, P < 0.05). The similar results were found in the familial biological kinship relatives with any HBV-infected makers.


CONCLUSIONS
VDR-Taq I and -Fok I gene polymorphisms are likely to play a substantial role in HBsAg familial aggregation.
Shan
Jing
J
Institute of Hepatitis Research, Affiliated You'an Hospital, Capital University of Medical Sciences, Beijing 100096, China.
Wang
Lu
L
Li
Zhuo
Z
Liu
Ying
Y
Gao
Ji-rong
JR
Pang
Yan-lei
YL
Li
Jun-hong
JH
Pang
Fu-min
FM
Guo
Xin-hui
XH
Chi
Hong-Zhi
HZ
Zhang
Chang-geng
CG
Li
Hui
H
D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006239:Haplotypes; D006509:Hepatitis B / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018167:Receptors, Calcitriol / Q000235:genetics*
D006514:Hepatitis B Surface Antigens; D018167:Receptors, Calcitriol