title: Genome-wide linkage of plasma adiponectin reveals a major locus on chromosome 3q distinct from the adiponectin structural gene: the IRAS family study.
abstract: Adiponectin (APM1) is an adipocyte-derived peptide that contributes to glucose, lipid, and energy homeostasis. We assessed the genetic basis of plasma adiponectin in Hispanic-American and African-American families enrolled through the Insulin Resistance Atherosclerosis Study Family Study. A 10-cM genome scan was performed in two batches: an original set (set 1) consisting of 66 families (45 Hispanic American and 21 African American) and a replication set (set 2) consisting of 66 families (45 Hispanic American and 21 African American). Adiponectin levels were measured by radioimmunoassay in 1,727 individuals from 131 of 132 families. Linkage analysis was carried out in Hispanic Americans and African Americans separately in set 1, set 2, and the pooled set (set 1 plus set 2), with and without diabetic subjects. A major gene was mapped to 3q27 with a logarithm of odds (LOD) score of 8.21 in the Hispanic-American sample. Ninety-six unrelated individuals were screened for polymorphisms in the APM1 gene, and 18 single nucleotide polymorphisms (SNPs) were genotyped in the Hispanic-American sample. Plasma adiponectin level was modestly associated with two SNPs and their accompaning haplotypes. Incorporating each or both SNPs in the linkage analysis, however, did not significantly reduce the LOD score. Therefore, a quantitative trait locus at 3q27, likely distinct from the APM1 gene, contributes to the variation of plasma adiponectin levels in the Hispanic-American population.
authors.affiliation: Medical Genetics Institutes, Cedars-Sinai Medical Center, 8700 Beverly Blvd., 665W, Los Angeles, CA 90048, USA. xiuqing.guo@cshs.org
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Guo
authors.forename: Mohammed F
authors.initials: MF
authors.lastname: Saad
authors.forename: Carl D
authors.initials: CD
authors.lastname: Langefeld
authors.forename: Adrienne H
authors.initials: AH
authors.lastname: Williams
authors.forename: Jinrui
authors.initials: J
authors.lastname: Cui
authors.forename: Kent D
authors.initials: KD
authors.lastname: Taylor
authors.forename: Jill M
authors.initials: JM
authors.lastname: Norris
authors.forename: Sujata
authors.initials: S
authors.lastname: Jinagouda
authors.forename: Christine H
authors.initials: CH
authors.lastname: Darwin
authors.forename: Braxton D
authors.initials: BD
authors.lastname: Mitchell
authors.forename: Richard N
authors.initials: RN
authors.lastname: Bergman
authors.forename: Beth
authors.initials: B
authors.lastname: Sutton
authors.forename: Y-D Ida
authors.initials: YD
authors.lastname: Chen
authors.forename: Lynne E
authors.initials: LE
authors.lastname: Wagenknecht
authors.forename: Donald W
authors.initials: DW
authors.lastname: Bowden
authors.forename: Jerome I
authors.initials: JI
authors.lastname: Rotter
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000328:Adult; D001741:Black or African American / Q000235:genetics; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D003920:Diabetes Mellitus / Q000097:blood / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D015894:Genome, Human; D005838:Genotype; D006239:Haplotypes; D006630:Hispanic or Latino / Q000235:genetics; D006801:Humans; D008126:Lod Score; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci / Q000235:genetics
chemical_list: D052242:Adiponectin