title: G-protein-coupled receptor microarrays for multiplexed compound screening.
abstract: Conventional assay methods for discovering and profiling drug-target interactions are typically developed on a target-by-target basis and hence can be cumbersome to enable and orchestrate. Herein the authors report a solid-state ligand-binding assay that operates in a multiplexed mode to report compound activity against a micorarray-configured panel of G-protein-coupled receptor (GPCR) targets. The pharmacological fidelity of the system is high, and its miniaturized "plug-and-play" format provides improved efficiency both in terms of execution time and reagent consumption. Taken together, these features make the system ideally suited to explore the structure-activity relationship of compounds across a broad region of target class space.
authors.affiliation: Corning Incorporated, Corning, NY, USA.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Hong
authors.forename: Brian L
authors.initials: BL
authors.lastname: Webb
authors.forename: Sadashiva
authors.initials: S
authors.lastname: Pai
authors.forename: Ann
authors.initials: A
authors.lastname: Ferrie
authors.forename: Jinlin
authors.initials: J
authors.lastname: Peng
authors.forename: Fang
authors.initials: F
authors.lastname: Lai
authors.forename: Joydeep
authors.initials: J
authors.lastname: Lahiri
authors.forename: Gloria
authors.initials: G
authors.lastname: Biddlecome
authors.forename: Brian
authors.initials: B
authors.lastname: Rasnow
authors.forename: Michael
authors.initials: M
authors.lastname: Johnson
authors.forename: Hosung
authors.initials: H
authors.lastname: Min
authors.forename: Ye
authors.initials: Y
authors.lastname: Fang
authors.forename: John
authors.initials: J
authors.lastname: Salon
mesh_terms: D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D040081:Protein Array Analysis / Q000379:methods*; D043562:Receptors, G-Protein-Coupled / Q000187:drug effects*
chemical_list: D005456:Fluorescent Dyes; D043562:Receptors, G-Protein-Coupled