title: RNA extraction.
abstract: RNA extraction is a routine technique in a molecular biology lab. High quality of RNA extracted from plants is a prerequisite to succeeding in subsequent experiments such as reverse transcription-polymerase chain reaction, Northern hybridization, cDNA library construction, and microarray analysis. Two methods for RNA extraction in Arabidopsis, small-scale and large-scale, are described here. RNA isolated by using the small-scale method can be applied in most downstream applications. The large-scale RNA preparation is recommended to extract RNA for Northern hybridization only.
authors.affiliation: Texas Tech University, Department of Chemistry and Biochemistry, Lubbock, USA.
authors.forename: Huazhong
authors.initials: H
authors.lastname: Shi
authors.forename: Ray
authors.initials: R
authors.lastname: Bressan
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D001901:Botany / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D005821:Genetic Techniques; D018749:RNA, Plant / Q000032:analysis* / Q000302:isolation & purification*; D012260:Ribonucleases / Q000037:antagonists & inhibitors
chemical_list: D018749:RNA, Plant; D012260:Ribonucleases