Developmental expression of Shisa-2 in Xenopus laevis.
Shisa is an antagonist of Wnt and FGF signaling, that functions cell autonomously in the endoplasmic reticulum (ER) to inhibit the post-translational maturation of Wnt and FGF receptors. In this paper we report the isolation of a second Xenopus shisa gene (Xshisa-2). Xenopus Shisa-2 shows 30.7% identity to Xshisa. RT-PCR analysis indicated that Xshisa-2 mRNA is present throughout early development and shows an increased expression during neurula and tailbud stages. At neurula stages Xenopus shisa-2 is initially expressed in the presomitic paraxial mesoderm and later in the developing somites. The expression profiles and pattern of Xshisa and Xshisa-2 differ significantly. During gastrulation only Xshisa mRNA is present in the Spemann-Mangold organizer and later on becomes restricted to the neuroectoderm and the prechordal plate.
Silva
Ana-Cristina
AC
Early Mouse Development Laboratory, Instituto Gulbenkian de Cincia, Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal.
Filipe
Mário
M
Vitorino
Marta
M
Steinbeisser
Herbert
H
Belo
José-António
JA
D000595:Amino Acid Sequence; D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D015398:Signal Transduction / Q000502:physiology; D051153:Wnt Proteins / Q000037:antagonists & inhibitors / Q000502:physiology; D029867:Xenopus Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D014982:Xenopus laevis
C524790:SHISA2 protein, Xenopus; D051153:Wnt Proteins; D029867:Xenopus Proteins; C524792:shisa1.1 protein, Xenopus; D005346:Fibroblast Growth Factors