title: Implementation of X-ray fluorescence microscopy for investigation of elemental abnormalities in amyotrophic lateral sclerosis.
abstract: The abnormalities of metallochemical reactions may contribute to the pathogenesis of Amyotrophic Lateral Sclerosis (ALS). In the present work, an investigation of the elemental composition of the gray matter, nerve cells and white matter from spinal cord tissues representing three ALS cases and five non-ALS controls was performed. This was done with the use of the synchrotron microbeam X-ray fluorescence technique (micro-SRXRF). The following elements were detected in the tissue sections: P, S, Cl, K, Ca, Fe, Cu, Zn and Br. A higher accumulation of Cl, K, Ca, Zn and Br was observed in the nerve cell bodies than in the surrounding tissue. Contrary to all other elements, Zn accumulation was lower in the white matter areas than in the gray matter ones. The results of quantitative analysis showed that there were no general abnormalities in the elemental accumulation between the ALS and the control group. However, for individual ALS cases such abnormalities were observed for the nerve cells. We also demonstrated differences in the elemental accumulation between the analyzed ALS cases.
authors.affiliation: Institute of Neurology, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Tomik
authors.forename: J
authors.initials: J
authors.lastname: Chwiej
authors.forename: M
authors.initials: M
authors.lastname: Szczerbowska-Boruchowska
authors.forename: M
authors.initials: M
authors.lastname: Lankosz
authors.forename: S
authors.initials: S
authors.lastname: Wójcik
authors.forename: D
authors.initials: D
authors.lastname: Adamek
authors.forename: G
authors.initials: G
authors.lastname: Falkenberg
authors.forename: S
authors.initials: S
authors.lastname: Bohic
authors.forename: A
authors.initials: A
authors.lastname: Simionovici
authors.forename: Z
authors.initials: Z
authors.lastname: Stegowski
authors.forename: A
authors.initials: A
authors.lastname: Szczudlik
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000378:metabolism*; D006219:Halogens / Q000032:analysis*; D006801:Humans; D008673:Metals, Alkaline Earth / Q000032:analysis*; D019216:Metals, Heavy / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods; D010758:Phosphorus / Q000032:analysis*; D011188:Potassium / Q000032:analysis*; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D013116:Spinal Cord / Q000737:chemistry; D013455:Sulfur / Q000032:analysis*; D017356:Synchrotrons; D014965:X-Rays
chemical_list: D006219:Halogens; D008673:Metals, Alkaline Earth; D019216:Metals, Heavy; D010758:Phosphorus; D013455:Sulfur; D011188:Potassium