Impedance spectroscopy of alpha-beta tubulin heterodimer suspensions.
Impedance spectroscopy is a technique that reveals information, such as macromolecular charges and related properties about protein suspensions and other materials. Here we report on impedance measurements over the frequency range of 1 Hz to 1 MHz of alpha-beta tubulin heterodimers suspended in a buffer. These and other polyelectrolyte suspensions show enormous dielectric responses at low frequencies, due both to the motion of charges suspended in the medium and to an electrical double layer that forms at each electrode-medium interface. We propose an equivalent circuit model to minimize electrode polarization effects and extract the intrinsic response of the bulk medium. At megaHertz frequencies, the conductivity increases with concentration below the critical concentration of approximately 1 mg/ml for microtubule polymerization, above which the conductivity decreases. This suggests that such measurements can be used to monitor the dynamics of microtubule polymerization. Finally, we obtain the net charge number per tubulin dimer of /Z/ = 306 in the saline buffer, which, if maintained as the dimers polymerized, would yield a linear charge density of 3.8 e/angstroms for the assembled microtubules. These results are potentially important for fundamental electrostatic processes in biomolecules and suggest the possibility of developing future bioelectronic applications.
Sanabria
Hugo
H
Department of Physics and Texas Center for Superconductivity at University of Houston, Houston, Texas 77204, USA.
Miller
John H
JH
Mershin
Andreas
A
Luduena
Richard F
RF
Kolomenski
Alexandre A
AA
Schuessler
Hans A
HA
Nanopoulos
Dimitri V
DV
D001665:Binding Sites; D003198:Computer Simulation; D019281:Dimerization; D017097:Electric Impedance; D008956:Models, Chemical; D046912:Multiprotein Complexes / Q000737:chemistry / Q000648:ultrastructure; D011485:Protein Binding; D011487:Protein Conformation; D012996:Solutions; D013057:Spectrum Analysis / Q000379:methods*; D014404:Tubulin / Q000737:chemistry* / Q000648:ultrastructure*
D046912:Multiprotein Complexes; D012996:Solutions; D014404:Tubulin