Cardiovascular malformations induced by prenatal exposure to phenobarbital in rats.
The effects of prenatal exposure to phenobarbital (PB) on the cardiovascular system were examined in rat fetuses and pups. PB was administered at a dose of 80 or 120 mg/kg/day by gavage to Sprague Dawley (SD) rats on two consecutive gestational days (GD): 7-8, 8-9, 9-10, or 10-11. Fetuses were examined for cardiovascular malformations on GD 20. In addition, pups were examined for PB-induced cardiovascular malformations. Incidences of ventricular septal defect (VSD), overriding aorta, double outlet right ventricle and transposition of great arteries were significantly increased in the fetuses whose dams were administered PB at 120 mg/kg on GD 8-9, 9-10 or 10-11. GD 8-11 was the critical period for the cardiovascular malformations associated with administration of PB in rats. Various types of cardiovascular malformations were detected in pups from the PB-administered dam. Severe cardiovascular malformations induced by PB caused deaths on early postnatal days. However, slight malformations such as isolated VSD persisted until weaning, and did not affect postnatal viability.
Okuda
Hirokazu
H
Japan Bioassay Research Center, Japan Industrial Safety and Health Association, Kanagawa, Japan. h-okuda@jisha.or.jp
Nagao
Tetsuji
T
D000014:Abnormalities, Drug-Induced; D000818:Animals; D018376:Cardiovascular Abnormalities / Q000139:chemically induced; D004305:Dose-Response Relationship, Drug; D018691:Excitatory Amino Acid Antagonists / Q000633:toxicity*; D005260:Female; D006330:Heart Defects, Congenital / Q000139:chemically induced*; D008297:Male; D008431:Maternal-Fetal Exchange; D010634:Phenobarbital / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D018691:Excitatory Amino Acid Antagonists; D010634:Phenobarbital