title: [Experimental study on cell adhesion characteristic between porous biphasic calcium phosphate nanocomposite and bone marrow mesenchymal stem cells in vitro].
abstract: OBJECTIVE
  To study the adhesion characteristic in vitro between porous biphasic calcium phosphate (BCP) nanocomposite and bone marrow mesenchymal stem cells (MSCs) that have been induced and proliferated.
  
  
  METHODS
  MSCs obtained from SD rat bone marrow were in vitro induced and proliferated. After their osteoblastic phenotype were demonstrated, MSCs were seeded onto prepared porous BCP nanocomposite (experiment group)and common porous hydroxyapatite (control group). Their adhesion situation was analyzed by scanning electron microscope. The initial optimal cell seeding density was investigated between new pattern porous BCP nanocomposite and MSCs by MTT automated colorimetric microassay method.
  
  
  RESULTS
  The differentiation of MSCs to osteoblastic phenotype were demonstrated by the positive staining of mineralized node, alkaline phosphatase (ALP) and collagen type I, the most appropriate seeding density between them was 2 X 10(6)/ml. The maximal number which MSCs could adhere to porous BCP nanocomposite was 1.28 X 10(7)/cm3.
  
  
  CONCLUSIONS
  MSCs can differentiate to osteoblastic phenotype. The MSCs were well adhered to porous BCP nanocomposite.
authors.affiliation: Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu Sichuan, 610041, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Weidong
authors.initials: W
authors.lastname: Tian
authors.forename: Xizhe
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002130:Calcium Phosphates / Q000494:pharmacology*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D053761:Nanocomposites; D010006:Osteoblasts / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells; D023822:Tissue Engineering / Q000379:methods
chemical_list: D002130:Calcium Phosphates; C020243:calcium phosphate