title: Identification of novel quaternary domain interactions in the Hsp90 chaperone, GRP94.
abstract: The structural basis for the coupling of ATP binding and hydrolysis to chaperone activity remains a central question in Hsp90 biology. By analogy to MutL, ATP binding to Hsp90 is thought to promote intramolecular N-terminal dimerization, yielding a molecular clamp functioning in substrate protein activation. Though observed in studies with recombinant domains, whether such quaternary states are present in native Hsp90s is unknown. In this study, native subunit interactions in GRP94, the endoplasmic reticulum Hsp90, were analyzed using chemical cross-linking in conjunction with tandem mass spectrometry. We report the identification of two distinct intermolecular interaction sites. Consistent with previous studies, one site comprises the C-terminal dimerization domain. The remaining site represents a novel intermolecular contact between the N-terminal and middle (M) domains of opposing subunits. This N+M domain interaction was present in the nucleotide-empty, ADP-, ATP-, or geldanamycin-bound states and could be selectively disrupted upon addition of synthetic geldanamycin dimers. These results identify a compact, intertwined quaternary conformation of native GRP94 and suggest that intersubunit N+M interactions are integral to the structural biology of Hsp90.
authors.affiliation: Mass Spectrometry Facility, University of California, San Francisco, California 94143, USA.
authors.forename: Feixia
authors.initials: F
authors.lastname: Chu
authors.forename: Jason C
authors.initials: JC
authors.lastname: Maynard
authors.forename: Gabriela
authors.initials: G
authors.lastname: Chiosis
authors.forename: Christopher V
authors.initials: CV
authors.lastname: Nicchitta
authors.forename: Alma L
authors.initials: AL
authors.lastname: Burlingame
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D016227:Benzoquinones; D002853:Chromatography, Liquid / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D019281:Dimerization; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D047029:Lactams, Macrocyclic; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011809:Quinones / Q000378:metabolism; D013388:Succinimides / Q000737:chemistry; D013552:Swine
chemical_list: D016227:Benzoquinones; D003432:Cross-Linking Reagents; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D008565:Membrane Proteins; D018832:Molecular Chaperones; D011809:Quinones; D013388:Succinimides; C018551:glucose-regulated proteins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C019358:disuccinimidyl suberate; C001277:geldanamycin