Absence of specific follicle stimulating hormone receptors in porcine corpora lutea.
Crude cell membrane preparations of corpora lutea from 13 pigs in different phases of the estrous cycle or pregnancy were assayed for the presence of specific follicle stimulating hormone (FSH) receptors using highly purified ovine FSH. Testicular tissue from boars and granulosa cells from porcine follicles served as positive controls. Scatchard analysis was used to determine binding affinity of FSH to target tissues as well to study human chorionic gonadotropin (hCG) bindability to corpora lutea membranes. In contrast to testicular tissue and granulosa cells, no specific FSH binding was detected in luteal tissue during the estrous cycle or pregnancy in pigs.
Ziecik
A J
AJ
Department of Animal Science, North Carolina State University, Box 7621 Raleigh, NC 27695-7621 USA.
Esbenshade
K L
KL
Britt
J H
JH