Full-field quantitative phase imaging by white-light interferometry with active phase stabilization and its application to biological samples.
We report a Koehler-illumination-based full-field, actively stabilized, low-coherence phase-shifting interferometer, which is built on a white-light Michelson interferometer. By using a phase-stepping technique we can obtain full-field phase images of the sample. An actively stabilized phase-lock circuit is employed in the system to reduce phase noise. An application to human epithelial cells (HeLa cells) is achieved in our experiment. The advancement of this technique rests in its ability to take images of unstained biological samples quantitatively and on a nanometer scale.
Li
Xinhong
X
State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, China.
Yamauchi
Toyohiko
T
Iwai
Hidenao
H
Yamashita
Yutaka
Y
Zhang
Haijun
H
Hiruma
Teruo
T
D004867:Equipment Design; D019544:Equipment Failure Analysis; D005246:Feedback; D006367:HeLa Cells; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D008027:Light; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014159:Transducers