The effect of semen extension, cAMP and caffeine on in vitro fertilization of bovine oocytes.
A previously reported in vitro system that used epididymal spermatozoa for fertilizing bovine follicular oocytes (1) has been expanded to include ejaculated semen as the sperm source. Frequency of fertilization was higher when semen was extended 1:1 prior to transport to the laboratory rather than transport as neat semen. Pretreatment of spermatozoa with cAMP, caffeine or both prior to insemination of oocytes did not increase frequency of either acrosome reactions or fertilization after sperm/oocyte incubation.
Critser
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ES
Department of Meat and Animal Science University of Wisconsin Madison, WI 53706 USA.
Leibfried
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ML
First
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NL