title: Critical role of MCP-1 in the pathogenesis of experimental colitis in the context of immune and enterochromaffin cells.
abstract: Mucosal changes in inflammatory bowel disease (IBD) are characterized by ulcerative lesions accompanied by a prominent infiltrate of inflammatory cells including lymphocytes, macrophages, and neutrophils and alterations in 5-hydroxytryptamine (5-HT)-producing enterochromaffin (EC) cells. Mechanisms involved in recruiting and activating these cells are thought to involve a complex interplay of inflammatory mediators. Studies in clinical and experimental IBD have shown the upregulation of various chemokines including monocyte chemoattractant protein (MCP)-1 in mucosal tissues. However, precise information on the roles of this chemokine or the mechanisms by which it takes part in the pathogenesis of IBD are not clear. In this study, we investigated the role of MCP-1 in the development of hapten-induced experimental colitis in mice deficient in MCP-1. Our results showed a significant reduction in the severity of colitis both macroscopically and histologically along with a decrease in mortality in MCP-1-deficient mice compared with wild-type control mice. This was correlated with a downregulation of myeloperoxidase activity, IL-1beta, IL-12p40, and IFN-gamma production, and infiltration of CD3+ T cells and macrophages in the colonic mucosa. In addition, we observed significantly lower numbers of 5-HT-expressing EC cells in the colon of MCP-1-deficient mice compared with those in wild-type mice after dinitrobenzenesulfonic acid. These results provide evidence for a critical role of MCP-1 in the development of colonic inflammation in this model in the context of immune and enteric endocrine cells.
authors.affiliation: Intestinal Disease Research Program, Department of Medicine, McMaster University, 1200 Main St. West, Hamilton, ON, Canada L8N 3Z5. khanwal@mcmaster.ca
authors.forename: W I
authors.initials: WI
authors.lastname: Khan
authors.forename: Y
authors.initials: Y
authors.lastname: Motomura
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: R T
authors.initials: RT
authors.lastname: El-Sharkawy
authors.forename: E F
authors.initials: EF
authors.lastname: Verdu
authors.forename: M
authors.initials: M
authors.lastname: Verma-Gandhu
authors.forename: B J
authors.initials: BJ
authors.lastname: Rollins
authors.forename: S M
authors.initials: SM
authors.lastname: Collins
mesh_terms: D000818:Animals; D017252:CD3 Complex; D018932:Chemokine CCL2 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D003092:Colitis / Q000139:chemically induced / Q000473:pathology*; D016207:Cytokines / Q000378:metabolism; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives; D004759:Enterochromaffin Cells / Q000502:physiology*; D007111:Immunity, Cellular / Q000502:physiology; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000096:biosynthesis; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009195:Peroxidase / Q000378:metabolism; D012701:Serotonin / Q000096:biosynthesis; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D017252:CD3 Complex; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D016207:Cytokines; C045305:2,4-dinitrofluorobenzene sulfonic acid; D012701:Serotonin; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene; D009195:Peroxidase