title: The complete nucleotide sequence and genomic organization of Citrus Leprosis associated Virus, Cytoplasmatic type (CiLV-C).
abstract: The Citrus leprosis disease (CiL) is associated to a virus (CiLV) transmitted by Brevipalpus spp. mites (Acari: Tenuipalpidae). CiL is endemic in Brazil and its recently spreading to Central America represents a threat to citrus industry in the USA. Electron microscopy images show two forms of CiLV: a rare nuclear form, characterized by rod-shaped naked particle (CiLV-N) and a common cytoplasmic form (CiLV-C) associated with bacilliform-enveloped particle and cytoplasmic viroplasm. Due to this morphological feature, CiLV-C has been treated as Rhabdovirus-like. In this paper we present the complete nucleotide sequence and genomic organization of CiLV-C. It is a bipartite virus with sequence similarity to ssRNA positive plant virus. RNA1 encodes a putative replicase polyprotein and an ORF with no known function. RNA2 encodes 4 ORFs. pl5, p24 and p61 have no significant similarity to any known proteins and p32 encodes a protein with similarity to a viral movement protein. The CiLV-C sequences are associated with typical symptoms of CiL by RT-PCR. Phylogenetic analysis suggests that CiLV-C is probably a member of a new family of plant virus evolutionarily related to Tobamovirus.
authors.affiliation: Alellyx Applied Genomics, Techno Park (Rod. Anhanguera Km 104), Rua James Clerk Maxwell 320, 13069-380, Campinas, SP, Brazil.
authors.forename: Renata C
authors.initials: RC
authors.lastname: Pascon
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Kitajima
authors.forename: Michèle C
authors.initials: MC
authors.lastname: Breton
authors.forename: Laura
authors.initials: L
authors.lastname: Assumpção
authors.forename: Christian
authors.initials: C
authors.lastname: Greggio
authors.forename: Almir S
authors.initials: AS
authors.lastname: Zanca
authors.forename: Vagner Katsumi
authors.initials: VK
authors.lastname: Okura
authors.forename: Marcos C
authors.initials: MC
authors.lastname: Alegria
authors.forename: Maria E
authors.initials: ME
authors.lastname: Camargo
authors.forename: Giovana G C
authors.initials: GG
authors.lastname: Silva
authors.forename: Jussara C
authors.initials: JC
authors.lastname: Cardozo
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Vallim
authors.forename: Sulamita F
authors.initials: SF
authors.lastname: Franco
authors.forename: Vitor H
authors.initials: VH
authors.lastname: Silva
authors.forename: Hamilton
authors.initials: H
authors.lastname: Jordão
authors.forename: Fernanda
authors.initials: F
authors.lastname: Oliveira
authors.forename: Poliana F
authors.initials: PF
authors.lastname: Giachetto
authors.forename: Fernanda
authors.initials: F
authors.lastname: Ferrari
authors.forename: Carlos I
authors.initials: CI
authors.lastname: Aguilar-Vildoso
authors.forename: Fabrício J B
authors.initials: FJ
authors.lastname: Franchiscini
authors.forename: José M F
authors.initials: JM
authors.lastname: Silva
authors.forename: Paulo
authors.initials: P
authors.lastname: Arruda
authors.forename: Jesus A
authors.initials: JA
authors.lastname: Ferro
authors.forename: Fernando
authors.initials: F
authors.lastname: Reinach
authors.forename: Ana Cláudia Rasera
authors.initials: AC
authors.lastname: da Silva
mesh_terms: D001483:Base Sequence; D032084:Citrus sinensis / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000145:classification / Q000235:genetics*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral