title: Character of preovulatory follicles and oocytes after different superovulatory treatments in heifers.
abstract: The relationship between the opaqueness of the surface of bovine preovulatory follicles, degree of expansion of oocyte-cumulus investment, presence of perivitelline space, and abstriction of the first polar body was examined in heifers treated with PMSG (Group I), FSH-P (Group II), and FSH-P/GnRH (Group III). Follicles greater than 8 mm in diameter were inspected by laparoscopy 65 hours after treatment with cloprostenol in all groups and were classified as clear or opaque based on their surface appearance. Subsequently, oocytes were recovered from the follicles and characterized. The proportion of clear exceeded that of opaque follicles in all groups. The oocyte recovery rate was highest for clear follicles in Group I and II, while in Group III the rates were identical for clear and opaque follicles. The proportion of oocytes with expanded cumulus investment was highest in Group III. In Group II and I decreased proportions of expansion was seen especially among oocytes from opaque follicles. The proportion of oocytes with perivitelline space was highest in Group II and III. Again, this effect was most pronounced among oocytes from "opaque" follicles. The proportion of oocytes with a polar body was highest in Group III followed by Group II while only few oocytes in Group I displayed a polar body. It is concluded that treatment with FSH-P and especially in combination with GnRH reduced the incidence of follicular atresia as measured by opaqueness and improved oocyte quality as indicated by cumulus expansion, formation of the perivitelline space, and abstriction of the first polar body.
authors.affiliation: Research Institute of Animal Production Department of Reproduction and Embryology, Section for Embryology Hlohovska 2, 949 92 Nitra, Czechoslovakia.
authors.forename: J
authors.initials: J
authors.lastname: Laurincik
authors.forename: P
authors.initials: P
authors.lastname: Grafenau
authors.forename: P
authors.initials: P
authors.lastname: Hyttel
authors.forename: T
authors.initials: T
authors.lastname: Greve