Mobilisation into cotton and spread of a recombinant cotton leaf curl disease satellite.
Analysis of a DNA beta satellite associated with a recently identified cotton leaf curl disease (CLCuD) strain indicated it to be recombinant, with most of the molecule originating from CLCuD DNA beta but with some sequence from a satellite isolated from tomato. Analysis of both archival (pre 2001) and recent cotton samples, shows the recombinant satellite is confined to a small area but was not present in cotton prior to 2001. This indicates that the recombinant DNA beta was recently mobilized into cotton, likely from tomato, and that recombination plays a role in the evolution of these satellites.
Amin
I
I
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. imran@nibge.org
Mansoor
S
S
Amrao
L
L
Hussain
M
M
Irum
S
S
Zafar
Y
Y
Bull
S E
SE
Briddon
R W
RW
D001483:Base Sequence; D005075:Biological Evolution; D004276:DNA, Satellite / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D017791:Geminiviridae / Q000235:genetics* / Q000302:isolation & purification; D003368:Gossypium / Q000821:virology*; D008969:Molecular Sequence Data; D010154:Pakistan; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D011995:Recombination, Genetic; D016415:Sequence Alignment
D004276:DNA, Satellite; D004279:DNA, Viral