[Primary study on the anti-tumor effect of ethanol extracts of Solanum lyratum].
OBJECTIVE
To observe the anti-tumor activity of the ethanol extracts of Solanun lyratum in vitro and in vivo.


METHODS
In vitro, the inhibitory effects of ethanol extracts of S. lyratum on proliferation of human hepatoma BEL-7402 cell and gastric carcinoma SGC-7901 cell were measured by MTT colorimetric assay. The mouse tumor model was used to investigate the effects of ethanol extracts on tumor growth.


RESULTS
The studies demonstrated that ethanol extracts of S. lyratum inhibited proliferation of BEL-7402 cells and SGC-7901 cells, and the IC50 values on them were (287.40 +/- 5.84) micron x mL(-1) and (176.14 +/- 5.18) microg x mL(-1), respectively. The tumor inhibitory rate of high doses of ethanol extracts on S180 sarcoma-transplanted mice and H22 hepatic cancer were (41.15 +/- 4.54) % and (45.00 +/- 7.37) %, respectively. When the dose of ethanol extracts varied from low to high, it was able to inhibit the growth of S180 sarcoma-transplanted mice and H22 hepatic cancer in a dose-dependent manner.


CONCLUSIONS
In tumor inhibitory test, it was shown that the ethanol extracts of S. lyratum may possess significantly inhibitory effect in vitro and in vivo. No acute toxic effect was found in our experiment.
Ren
Jing
J
College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Feng
Guo-Nan
GN
Wang
Min-Wei
MW
Sun
Li-Xin
LX
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010946:Plants, Medicinal / Q000737:chemistry; D012510:Sarcoma 180 / Q000473:pathology*; D032322:Solanum / Q000737:chemistry; D013274:Stomach Neoplasms / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D000431:Ethanol