Hormone profiling in Arabidopsis.
A highly sensitive and accurate multiplex gas chromatography-mass spectrometry (GC-MS/MS)-technique is reported for the quantitative analysis of acidic phytohormones in Arabidopsis thaliana and other plant species. The optimized setup allows the routine processing and analysis of between 20 mg and 5 g of tissue. The protocol was designed and the equipment used was chosen to facilitate implementation of the method into other laboratories and to provide access to state-of-the-art analytical tools for the acidic phytohormones and related signaling molecules.
Müller
Axel
A
CarboGen Laboratories AG, Aarau, Switzerland.
Düchting
Petra
P
Weiler
Elmar W
EW
D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D003978:Diazomethane / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007210:Indoleacetic Acids / Q000494:pharmacology; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis; D020156:Salicylic Acid / Q000378:metabolism
D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D003978:Diazomethane; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; C030737:indoleacetic acid; D000040:Abscisic Acid; D020156:Salicylic Acid