title: Effect of ethanolic extracts of Ananas comosus L. leaves on insulin sensitivity in rats and HepG2.
abstract: Ethanolic extracts of Ananas comosus L. leaves (AC) enriched with phenols have hypoglycemic activity in diabetic rats. Here, we investigated the effect of AC on insulin sensitivity in rats and HepG2. In high-fat diet-fed and low-dose streptozotozin-treated diabetic Wistar rats subjected to challenge with exogenous human insulin, AC treatment at an oral dose of 0.40 g/kg could significantly improve sensitivity to exogenous insulin. After a sub-acute treatment, AC also could inhibit the development of insulin resistance in high-fat diet-fed and low-dose streptozotozin-treated diabetic rats following the test of loss of tolbutamide-induced blood glucose lowering action. For intravenous insulin/glucose infusion test, high-fat diet-fed and low-dose alloxan-treated Wistar rats were associated with insulin resistance, which was improved after AC or fenofibrate treatment. AC application inhibited the development of insulin resistance in HepG2 cells. The above animal models were well developed to simulate type 2 diabetes. Taken together, our results suggest that AC may improve insulin sensitivity in type 2 diabetes and could be developed into a new potential natural product for handling of insulin resistance in diabetic patients.
authors.affiliation: Life Sciences Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Su
authors.forename: Dongming
authors.initials: D
authors.lastname: Xing
authors.forename: Yang
authors.initials: Y
authors.lastname: Pan
authors.forename: Lijun
authors.initials: L
authors.lastname: Du
mesh_terms: D031230:Ananas / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy; D000431:Ethanol; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D010936:Plant Extracts; D000431:Ethanol; D006003:Glycogen; D005947:Glucose