Surfactin C inhibits platelet aggregation.
This study was designed to investigate the effect of surfactin C, which is derived from Bacillus subtilis, on platelet aggregation and homotypic leucocyte aggregation. Surfactin C strongly and dose-dependently inhibited platelet aggregation, which was stimulated both by thrombin (0.1 U mL(-1)), a potent agonist that activates the G protein-coupled protease receptor, and by collagen (5 microg mL(-1)), a potent ligand that activates alpha(IIb)beta(3) with IC50 values (concentration inhibiting platelet aggregation by 50%) of 10.9 and 17.0 microM, respectively. Moreover, surfactin C significantly suppressed the intracellular Ca(2+) mobilization in thrombin-activated platelets. Surfactin C, however, did not affect various integrin-mediated U937 cell aggregation, implying that the anti-platelet activity of surfactin C was not due to its detergent effect but by its action on the downstream signalling pathway. Therefore, the results suggest that surfactin C may have a beneficial therapeutic effect on aberrant platelet aggregation-mediated cardiovascular diseases.
Kim
Sung Dae
SD
Laboratory of Veterinary Physiology & Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
Park
Sun Kyu
SK
Cho
Jae Youl
JY
Park
Hwa Jin
HJ
Lim
Jong Hwan
JH
Yun
Hyo In
HI
Park
Seung Chun
SC
Lee
Kyoung Youl
KY
Kim
Sang Keun
SK
Rhee
Man Hee
MH
D000818:Animals; D002118:Calcium / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D055666:Lipopeptides; D010456:Peptides, Cyclic / Q000494:pharmacology*; D010741:Phospholipases A / Q000502:physiology; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D013917:Thrombin / Q000494:pharmacology
D055666:Lipopeptides; D010456:Peptides, Cyclic; D010975:Platelet Aggregation Inhibitors; C009365:surfactin peptide; D003094:Collagen; D010741:Phospholipases A; D013917:Thrombin; D002118:Calcium