title: Successful culmination of pregnancy and live birth following the transfer of frozen-thawed buffalo embryos.
abstract: A total of 141 embryos was recovered by nonsurgical flushing of the uterus of 31 superovulated buffalo. A total of 66 good quality embryos (Grade I and Grade II) was frozen using 1.4 M glycerol. Forty-two of the frozen embryos were thawed randomly over a 1-year period, and a total of 39 embryos (Grades I, II or III post thaw) were transferred into an equal number of estrus synchronized recipients. Of 11 confirmed pregnancies, 9 calves were born live.
authors.affiliation: Buffalo Breeding Centre, Nekarikallu - 522 615 (A.P.), India.
authors.forename: R
authors.initials: R
authors.lastname: Kasiraj
authors.forename: A K
authors.initials: AK
authors.lastname: Misra
authors.forename: M
authors.initials: M
authors.lastname: Mutha Rao
authors.forename: R S
authors.initials: RS
authors.lastname: Jaiswal
authors.forename: N S
authors.initials: NS
authors.lastname: Rangareddi