[Expression of Nestin, CK19, insulin, glucagon and somatostatin in embryonic pancreas].
OBJECTIVE
To investigate the expression of Nestin, CK19, insulin, glucagon and somatostatin in the development of embryonic pancreas.


METHODS
Sample collection was conducted in accordance to the principle of informed consent. Immunohistochemical SABC method and LAB-SA for double satining were used to study the expression of the molecules in human embryonic pancreas.


RESULTS
(1) There are few nestin-positive cells in the stroma of the embryonic pancreas. CK19 is consistently expressed in the epithelial cells of pancreatic ducts. (2) From 7 weeks of gestation, pancreatic duct epithelial cells begin to differentiate into insulin, glucagon and somatostatin-positive cells.


CONCLUSIONS
Nestin-positive pancrease stem cells are in the stroma of human embryonic pancrease. Pancreatic duct epithelial cells differentiate into islet cells secreting insulin at the early stage of embryonic development.
Yan
Kai-ming
KM
Department of Anatomy, Preclinical and Forensic College of Sichuan University, Chengdu 610041, China. kming1965@163.com
Li
Ai-dong
AD
Yang
Hui-jun
HJ
Mei
Yan
Y
Zhou
Hong-ying
HY
D000818:Animals; D002454:Cell Differentiation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005934:Glucagon / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D053539:Keratin-19 / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D010179:Pancreas / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D011247:Pregnancy; D013004:Somatostatin / Q000378:metabolism*
D007328:Insulin; D007381:Intermediate Filament Proteins; D053539:Keratin-19; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D013004:Somatostatin; D005934:Glucagon