Species identification of non-tuberculous mycobacteria from humans and cattle of Chad.
In Chad, during a study on tuberculosis in humans and cattle, 52 non-tuberculous mycobacteria (NTM) strains were isolated. By means of INNO-LiPA, PRA-hsp65 amplification and sequencing of 16S rDNA, NTM species of 25/52 isolates were identified. M. fortuitum complex (8) was the most frequent species, followed by M. nonchromogenicum (4) and M. avium complex (4). PRA method could identify M. fortuitum 3rd variant among isolates derived from cattle specimens. This finding could confirm the existence of farcy in the Chadian cattle population as M. fortuitum 3rd variant and putitative pathogen M. farcinogenes can't be distinguished by the methods used in this study. Half of the NTM isolates could not be specified and we considered them as contaminants from the environment.
Diguimbaye-Djaibé
C
C
Laboratoire de Recherches Vétérinaires et Zootechniques de Farcha, N'Djaména, Chad.
Vincent
V
V
Schelling
E
E
Hilty
M
M
Ngandolo
R
R
Mahamat
H H
HH
Pfyffer
G
G
Baggi
F
F
Tanner
M
M
Zinsstag
J
J
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002596:Chad; D004269:DNA, Bacterial / Q000032:analysis*; D005784:Gene Amplification; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology*; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013045:Species Specificity
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011933:Reagent Kits, Diagnostic