title: Hypoxia differentially regulates the expression of neuroglobin and cytoglobin in rat brain.
abstract: Neuroglobin (Ngb) and Cytoglobin (Cygb) are new members of the globin family and display heterotopic expression patterns. To examine the effect of different hypoxia profiles on expression of Ngb and Cygb in rodent brain, rats were exposed to either sustained hypoxia (SH; 10% O(2)) or intermittent hypoxia (IH; 10% and 21% O(2) alternating every 90 s) for 1, 3, 7 and 14 days, and mRNA and protein expression of Ngb and Cygb were assessed in brain cortex. SH increased Ngb mRNA and protein expression throughout the exposure, while IH only elicited slight increases in Ngb expression at day 1. Neither SH nor IH elicited increases in Cygb expression. Thus, hypoxic stimulus presentation is a major determinant of the regulation of hypoxic sensitive genes such as Ngb. Furthermore, disparities between Ngb and Cygb responses to hypoxia further suggest that these two globins may play divergent roles in brain.
authors.affiliation: Kosair Children's Hospital Research Institute, Department of Pediatrics, University of Louisville, KY 40202, USA.
authors.forename: Richard C
authors.initials: RC
authors.lastname: Li
authors.forename: Seung Kwan
authors.initials: SK
authors.lastname: Lee
authors.forename: Farzan
authors.initials: F
authors.lastname: Pouranfar
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Brittian
authors.forename: Heather B
authors.initials: HB
authors.lastname: Clair
authors.forename: Barry W
authors.initials: BW
authors.lastname: Row
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: David
authors.initials: D
authors.lastname: Gozal
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism; D000078842:Cytoglobin; D005914:Globins / Q000096:biosynthesis*; D002534:Hypoxia, Brain / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D000078787:Neuroglobin; D009687:Nuclear Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: C000627268:Cygb protein, rat; D000078842:Cytoglobin; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D009687:Nuclear Proteins; D012333:RNA, Messenger; D005914:Globins