title: Increased infiltration of macrophages in omental adipose tissue is associated with marked hepatic lesions in morbid human obesity.
abstract: In human obesity, white adipose tissue (WAT) is enriched in macrophages. How macrophage infiltration in WAT contributes to the complications of obesity is unknown. This study tested the hypothesis that recruitment of macrophages in omental WAT is associated with hepatic damage in obese patients. Paired biopsies of subcutaneous and omental WAT and a liver biopsy were collected during gastric surgery in 46 obese women and 9 obese men (BMI 47.9 +/- 0.93 kg/m(2)). The number of HAM56+ macrophages in WAT was quantified microscopically, and correlations with clinical and biological parameters and histological liver pathology were investigated. There were twice as many macrophages in omental as in subcutaneous WAT (P<0.0001). After adjustment for age, omental WAT macrophage infiltration was correlated to fasting glucose and insulin, quantitative insulin sensitivity check index, triglycerides, aspartate aminotransferase (AST), and gamma-glutamyltranspeptidase. We propose an easy equation to estimate the amount of macrophages in omental WAT. Increased macrophage accumulation specifically in omental WAT was associated with hepatic fibroinflammatory lesions (P=0.01). The best predictive model for the severity of hepatic damage includes adiponectinemia, AST, and omental WAT macrophages. These data suggest that the presence of macrophages in omental WAT participates in the cellular mechanisms favoring hepatic fibroinflammatory lesions in obese patients.
authors.affiliation: INSERM, U755 Nutriomique, Service de Nutrition, Hôtel-Dieu, 1 Place du Parvis Notre-Dame, 75004 Paris, France.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Cancello
authors.forename: Joan
authors.initials: J
authors.lastname: Tordjman
authors.forename: Christine
authors.initials: C
authors.lastname: Poitou
authors.forename: Gaël
authors.initials: G
authors.lastname: Guilhem
authors.forename: Jean Luc
authors.initials: JL
authors.lastname: Bouillot
authors.forename: Danielle
authors.initials: D
authors.lastname: Hugol
authors.forename: Christiane
authors.initials: C
authors.lastname: Coussieu
authors.forename: Arnaud
authors.initials: A
authors.lastname: Basdevant
authors.forename: Avner
authors.initials: A
authors.lastname: Bar Hen
authors.forename: Pierre
authors.initials: P
authors.lastname: Bedossa
authors.forename: Michèle
authors.initials: M
authors.lastname: Guerre-Millo
authors.forename: Karine
authors.initials: K
authors.lastname: Clément
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000473:pathology; D052436:Adipose Tissue, White / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000704:Analysis of Variance; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D008076:Cholesterol, HDL / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D016014:Linear Models; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009767:Obesity, Morbid / Q000378:metabolism / Q000473:pathology*; D009852:Omentum / Q000378:metabolism / Q000473:pathology*; D050151:Subcutaneous Fat / Q000378:metabolism / Q000473:pathology; D014280:Triglycerides / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D007328:Insulin; D014280:Triglycerides; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases