title: Cytokines and soluble intracellular adhesion molecule 1 in the sera of Saudi eczematous children.
abstract: We report five children with atopic eczema. The level of soluble adhesion molecules is known to correlate with disease severity. On the assumption that atopic eczema is a T-lymphocyte-mediated inflammatory disease, in this study, we aimed to determine the presence of soluble intracellular adhesion molecule (sICAM) 1 and the release of inflammatory cytokines. The patient sera were used to measure sICAM-1 by enzyme-linked immunosorbent assay, and the concentration of interleukin (IL)-4 and IL-5 in whole blood culture supernatant was determined by a flow cytometry microsphere-based assay. sICAM-1 was dramatically higher in these patients than healthy controls. In addition, the results showed an increase in IL-4 and IL-5 concentration compared with control subjects. In conclusion, these results showed that sICAM-1 expression is important in inflammation of epithelial cells and can be used as a useful parameter in monitoring the disease activity. The study suggests that T-helper cell subset plays an important role in initiating eczematous disease and that additional studies are needed to explore such a role.
authors.affiliation: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, Saudi Arabia. falenzi@kfu.edu.sa
authors.forename: Faris Q B
authors.initials: FQ
authors.lastname: Alenzi
mesh_terms: D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004485:Eczema / Q000097:blood* / Q000473:pathology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood*; D015848:Interleukin-5 / Q000097:blood*; D007958:Leukocyte Count; D012529:Saudi Arabia
chemical_list: D015848:Interleukin-5; D018799:Intercellular Adhesion Molecule-1; D015847:Interleukin-4; D007073:Immunoglobulin E