Multivariate optimisation of the microwave-assisted extraction of oleuropein and related biophenols from olive leaves.
Microwave assistance is proposed for the first time in order to accelerate the extraction of biophenols from olive leaves. Under optimal working conditions, obtained using a multivariate methodology, complete extraction of the target analytes was achieved in 8 min. The extracts required no clean-up nor concentration prior to injection into a chromatograph-photodiode array detector assembly for individual separation-quantification. The optimal extractant (an 80:20 ethanol-water mixture) was also used in the development of a stirring-based extraction method which required around 24 h for complete extraction of the target compounds. These mixtures can be used as replacements for toxic extractants, with a view to exploiting olive leaves in order to obtain biophenols for human use.
Japón-Luján
R
R
Analytical Chemistry Department, Annex Marie Curie Building, Rabanales Campus, University of Córdoba, 14071 Córdoba, Spain.
Luque-Rodríguez
J M
JM
Luque de Castro
M D
MD
D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D057888:Iridoid Glucosides; D039823:Iridoids; D007700:Kinetics; D008872:Microwaves; D015394:Molecular Structure; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011714:Pyrans / Q000737:chemistry* / Q000302:isolation & purification*
D057888:Iridoid Glucosides; D039823:Iridoids; D010636:Phenols; D010936:Plant Extracts; D011714:Pyrans; C002769:oleuropein