Potentiometric assessment of Gram-negative bacterial permeabilization of tobramycin.
The construction and electrochemical response characteristics of beta-cyclodextrin-based sensor for determination of the amino glycosidic antibiotic, tobramycin, is described. The membrane incorporates carboxylated poly (vinyl chloride) as matrix polymer, tetrakis-fluorophenyl borate as fixed anionic site and nitrophenyloctylether as plasticizer. The uptake of aminoglycosidic drug was applied to determine permeability changes in the Gram-negative bacteria Escherichia coli as test organism and ethylenediamine tetraacetic acid as model permeabilizer. The sensor shows linear response over concentration range of 10(-2) to 10(-5)M tobramycin with cationic slope of mV per concentration decade and accuracy of 99.8+/-2.14%. The proposed method was successfully applied to the determination of tobramycin uptake, by 100 microl E. coli suspension in presence of up to 10 microg ml(-1) of EDTA solution, with average recovery of 99.3+/-2.77%. The obtained results were compared reasonably well with the data obtained using the compendial fluorimetric assay. The proposed potentiometric assay serves as a convenient method in analyzing and quantifying the effects of permeabilizers on Gram-negative bacteria upon treatment with tobramycin.
El-Kosasy
Amira M
AM
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. kosasy_2010@yahoo.com
D015374:Biosensing Techniques; D004926:Escherichia coli / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010539:Permeability; D010968:Plasticizers; D011199:Potentiometry / Q000379:methods*; D014031:Tobramycin / Q000032:analysis / Q000493:pharmacokinetics*; D047392:beta-Cyclodextrins / Q000494:pharmacology
D010968:Plasticizers; D047392:beta-Cyclodextrins; C031215:betadex; D014031:Tobramycin