Effect of AgK114 on picryl chloride-induced chronic contact hypersensitivity responses.
BACKGROUND
Mouse AgK114 (a glycosylphosphatidylinositol (GPI) anchored membrane-associated protein) expression is found in somatotrophs of the pituitary gland in correlation with the expression of growth hormone. In this study, the effects of AgK114 on the systemic immune response were examined in contact hypersensitivity (CHS) model mice.


METHODS
AgK114 was intraperitoneally injected into BALB/c mice that were sensitized and challenged with picryl chloride (PiCl). Serum IgE levels and the antigen-specific cytokine production by lymph node (LN) cells were examined.


RESULTS
The serum IgE levels in the CHS mice treated with 10 microg/head of AgK114 during the repeated challenge with PiCl were significantly decreased compared with those of the control mice. Moreover, IL-4 production by LN cells in response to 2,4,6-trinitrobenzene-sulfonic acid sodium salt-treated splenocytes was decreased in the AgK114-treated CHS mice compared with that of the control mice.


CONCLUSIONS
Our results suggest that systemic administration of AgK114 exerted immunoregulatory functions on the allergic responses, resulting in the inhibition of IgE production.
Arai
Chikako
C
Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., 675-1 Fujisaki, Okayama 702-8006, Japan. fujisaki@hayashibara.co.jp
Tatefuji
Tomoki
T
Mori
Tetsuya
T
Mizote
Akiko
A
Kohno
Keizo
K
Ohta
Tsunetaka
T
Fukuda
Shigeharu
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002908:Chronic Disease; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy* / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D017261:Glycosylphosphatidylinositols / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000097:blood; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007371:Interferon-gamma / Q000032:analysis; D016753:Interleukin-10 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D010853:Picryl Chloride / Q000633:toxicity*; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014302:Trinitrobenzenesulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D017261:Glycosylphosphatidylinositols; D011994:Recombinant Proteins; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D014302:Trinitrobenzenesulfonic Acid; D010853:Picryl Chloride