[Detection of cyclin E in lymph node metastases of laryngeal squamous cell carcinomas by laser scanning confocal microscopy].
OBJECTIVE
To study the expression of Cyclin E proteins in carcinogenesis and development of the metastasis of laryngeal squamous cell carcinomas (LSCC).


METHODS
The expression of Cyclin E protein were detected by laser scanning confocal microscopy (LSCM) and multiparameter flow cytometry (FCM) after immunofluorescence staining method in twenty-one surgically resected primary LSCC (7 LSCC with cervical lymph node metastasis, 14 LSCC without cervical lymph node metastasis).


RESULTS
The fluorescence intensity was 78.12 +/- 6.54 in LSCC with cervical lymph node metastasis and 74.93 +/- 8. 82 in LSCC without cervical lymph node metastasis (P > 0. 05); The fluorescence intensity in cervical lymph node metastasis was 93.65 +/- 7.48, it's more higher than that in cervical lymph node negative metastasis (P < 0.01) and both LSCC with cervical lymph node metastasis and without cervical lymph node metastasis (P < 0.05).


CONCLUSIONS
It is suggested that Cyclin E proteins may play an important role in the carcinogenesis and development of the lymph node metastases of LSCC. Detection of Cyclin E maybe becomes an indication to evaluating the lymph node metastases of LSCC.
Zhang
Xiaowen
X
Department of Otolaryngology-Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China.
Kong
Weijia
W
Hu
Bin
B
Wang
Zhenlin
Z
D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D019927:Cyclin E / Q000378:metabolism*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008198:Lymph Nodes / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D018613:Microscopy, Confocal; D009367:Neoplasm Staging
D019927:Cyclin E