title: Paired in vitro and in vivo comparison of apheresis platelet concentrates stored in platelet additive solution for 1 versus 7 days.
abstract: BACKGROUND
  To improve clinical access to platelet concentrates (PCs), prolonging the storage period is one alternative, provided that they are free from bacteria. The quality of platelets (PLTs) stored for 1 versus 7 days was compared by in vitro analyses and in vivo recovery and survival in blood donors.
  
  
  METHODS
  Apheresis PCs from 10 donors were divided and stored in PLT additive solution in 2 equal units for a paired comparison. PLTs in one unit were (111)In-labeled at 1 day of storage, and PLTs in the other unit were labeled after 7 days of storage. PLTs were injected into the donor after labeling and samples were drawn after 30, 60, and 150 minutes and thereafter once a day for 14 days for recovery and survival measurements.
  
  
  RESULTS
  PLT recovery on Day 7 was 80 percent of the recovery on Day 1 (p<0.05), and the survival on Day 7 was 65 percent of survival on Day 1 (p<0.005). No significant differences were seen regarding mean PLT volume (MPV), pH, pCO2, pO2, bicarbonate, or hypotonic shock response. Lactate increased and lactic dehydrogenase increased slightly, whereas glucose and ATP decreased, but not to a critical level. A significant increase in RANTES (110.7+/-76.6 vs. 277.6+/-50.8 pg/10(6) PLTs [p<0.005]) and PLT factor 4 (19.9+/-9.6 vs. 59.8+/-7.5 IU/10(6) PLTs [p<0.0001]) was noticed during storage.
  
  
  CONCLUSIONS
  Recovery and survival of PCs stored for 7 days decreased, but met suggested criteria. Analyzed in vitro parameters showed acceptable results. Randomized patient transfusion studies will provide additional verification of the suitability of 7-day storage of PLTs.
authors.affiliation: Department of Transfusion Medicine, Karolinska University Hospital at Huddinge, Stockholm, Sweden. agneta.shanwell@karolinska.se
authors.forename: Agneta
authors.initials: A
authors.lastname: Shanwell
authors.forename: Beatrice
authors.initials: B
authors.lastname: Diedrich
authors.forename: Cecilia
authors.initials: C
authors.lastname: Falker
authors.forename: Berit
authors.initials: B
authors.lastname: Jansson
authors.forename: Per
authors.initials: P
authors.lastname: Sandgren
authors.forename: Lars
authors.initials: L
authors.lastname: Sundkvist
authors.forename: Leif
authors.initials: L
authors.lastname: Svensson
authors.forename: Mervi
authors.initials: M
authors.lastname: Vesterinen
authors.forename: Hans
authors.initials: H
authors.lastname: Gulliksson
mesh_terms: D001774:Blood Chemical Analysis; D001782:Blood Donors; D001792:Blood Platelets / Q000166:cytology; D001793:Blood Preservation / Q000379:methods*; D001804:Blood Transfusion, Autologous; D002470:Cell Survival; D006801:Humans; D017713:Platelet Transfusion; D010983:Plateletpheresis / Q000379:methods* / Q000592:standards; D012996:Solutions; D013997:Time Factors
chemical_list: D012996:Solutions