Biological, biochemical, and molecular characterization of a new clinical Trichophyton rubrum isolate resistant to terbinafine.
We have characterized a new clinical strain of Trichophyton rubrum highly resistant to terbinafine but exhibiting normal susceptibility to drugs with other mechanisms of action. Resistance to terbinafine in this strain is caused by a missense mutation in the squalene epoxidase gene leading to the amino acid substitution F397L.
Osborne
Colin S
CS
Infectious Diseases, Novartis Institutes for BioMedical Research, Inc., Cambridge, MA 02139, USA. colin.osborne@novartis.com
Leitner
Ingrid
I
Hofbauer
Bettina
B
Fielding
Ceri A
CA
Favre
Bertrand
B
Ryder
Neil S
NS
D019943:Amino Acid Substitution; D000935:Antifungal Agents / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D025141:Drug Resistance, Fungal / Q000235:genetics*; D004875:Ergosterol / Q000096:biosynthesis; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D050603:Squalene Monooxygenase / Q000737:chemistry / Q000378:metabolism; D000077291:Terbinafine; D014249:Trichophyton / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism*
D000935:Antifungal Agents; D009281:Naphthalenes; D050603:Squalene Monooxygenase; D000077291:Terbinafine; D004875:Ergosterol