Use of fluorescent proteins as reporters.
Fluorescent proteins (FPs) have established themselves as valuable reporter proteins in plant molecular biology. Beside general background information about spectral properties, protein structure and maturation of different commonly used FPs, this chapter provides detailed protocols about cloning of suitable expression cassettes for GFP and DsRED and detection of FPs in Arabidopsis protoplasts and plants transiently or stably expressing these constructs by fluorescence microscopy.
Jach
Guido
G
Max Planck-Institut fuer Zuechtungsforschung, Koeln, Germany.
D017360:Arabidopsis / Q000235:genetics*; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D030821:Plants, Genetically Modified; D017433:Protein Structure, Secondary; D011523:Protoplasts / Q000378:metabolism
D008164:Luminescent Proteins; C413663:fluorescent protein 583; D049452:Green Fluorescent Proteins