title: Silencing of HIV by hairpin-loop-structured DNA oligonucleotide.
abstract: We describe inhibition of HIV replication by a partially double-stranded 54mer oligodeoxynucleotide, ODN, which consists of an antisense strand targeting the highly conserved polypurine tract, PPT, of HIV, and a second strand, compatible with triple-helix formation. Upon treatment of HIV-infected cells with ODN early after infection no viral nucleic acids, syncytia or p24 viral antigen expression was observed. The ODN-mediated effect was highly sequence-specific. The ODN against HIV-IIIB was effective preferentially against its homologous PPT and less against the PPT of HIV-BaL differing in two of 24 nucleotides and vice versa. It may be interesting mechanistically as an antiviral drug.
authors.affiliation: Institute of Medical Virology, University of Zurich, Switzerland. moelling@immv.unizh.ch
authors.forename: Karin
authors.initials: K
authors.lastname: Moelling
authors.forename: Susanne
authors.initials: S
authors.lastname: Abels
authors.forename: Joerg
authors.initials: J
authors.lastname: Jendis
authors.forename: Alexey
authors.initials: A
authors.lastname: Matskevich
authors.forename: Jochen
authors.initials: J
authors.lastname: Heinrich
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D020868:Gene Silencing; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication
chemical_list: D017931:DNA Primers; D004279:DNA, Viral