title: [A study on the karyotype, C-banding and Ag-NORs in Rana nigromaculata].
abstract: The chromosome samples of Rana nigromaculata were prepared by peripheral blood lymphocyte cultured in vitro. Karyotype, C-banding and silver-staining nucleolus organizer regions (Ag-NORs) were observed on chromosomes samples. The result showed that: (1) the chromosome number of Rana nigromaculata is 26 (2n=26), i.e., 10 large and 16 small chromosomes, the large chromosomes are matched into 5 homologous pairs and small chromosomes are matched into 8 homologous pairs, and further indicates the karyotype of Rana nigromaculata is bi-modal; (2) Metaphases of female and male were observed separately, the chromosome of No.11 has a pronounced secondary constriction in the middle of long arms. But the position of secondary constriction in the aberrant type individuals is in the middle of long arms of the No.8; (3) it has only one obvious C-banding close to the telomere, which is located in the long arms of the No.5; (4) The chromosome No.11 is a pair of homologous chromosomes with Ag-NORs, and the position of Ag-NORs in female and male is identical.
authors.affiliation: School of Life Science, Xuzhou Normal University, Xuzhou 221116, China.
authors.forename: Heng-Liang
authors.initials: HL
authors.lastname: Shi
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Mao
authors.initials: M
authors.lastname: Wu
authors.forename: Pei-Qing
authors.initials: PQ
authors.lastname: Li
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Dong
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Pang
authors.forename: Huan-Min
authors.initials: HM
authors.lastname: Liu
authors.forename: Xing-Tang
authors.initials: XT
authors.lastname: Fang
authors.forename: Jian Guo
authors.initials: JG
authors.lastname: Gao
authors.forename: Bi-Cai
authors.initials: BC
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D005260:Female; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region / Q000235:genetics*; D011898:Ranidae / Q000235:genetics*; D016622:Silver Staining