Are fluorescent bodies of Y-spermatozoa detectable in common with mammalan species?
An attempt was made to detect the fluorescent bodies (F-body), using Quinacrine mustard (Q-M) staining in the spermatozoa from eight mammalian species (human, bull, boar, dog, rabbit, rat, mouse, and mastomys) as well as in the cock (used as negative control). Sperm suspension, prepared after rinsing by repeated centrifugation with phosphate buffered saline (PBS), was either stained with Q-M for 24 h or treated with protease and then stained with Q-M for 60 min. The final concentration of Q-M in the mixed staining sperm suspension was 0.025 mg/ml. The examination using a reflecting fluorescent microscope revealed that the F-body found in human sperm was also present in the sperm of all the mammals but not in the cock after 24 h of staining. The enzyme-treated specimens showed higher incidences of F-bodies than specimens stained for 24 h without enzymatic digestion. These findings strongly suggest that the F-body is commonly present in the spermatozoa of many mammalian species.
Ogawa
S
S
Labolatory of Animal Reproduction, Ikuta-Campus, Meiji University, Higashimita 1-1-1, Tama-ku, Kawasaki 214, Japan.
Yamakawa
H
H
Yamanoi
J
J
Nishida
S
S
Kano
Y
Y
Takeshima
T
T
Tauchi
K
K
Nagashima
H
H