Characterization of acatalasemic erythrocytes treated with low and high dose hydrogen peroxide. Hemolysis and aggregation.
The effects of hydrogen peroxide on normal and acatalasemic erythrocytes were examined. Severe hemolysis of acatalasemic erythrocytes and a small tyrosine radical signal (g = 2.005) associated with the formation of ferryl hemoglobin were observed upon the addition of less than 0.25 mM hydrogen peroxide. However, when the concentration of hydrogen peroxide was increased to 0.5 mM, acatalasemic erythrocytes became insoluble in water and increased the tyrosine radical signal. Polymerization of hemoglobin and aggregation of the erythrocytes were observed. On the other hand, normal erythrocytes exhibited only mild hemolysis by the addition of hydrogen peroxide under similar conditions. From these results, the scavenging of hydrogen peroxide by hemoglobin generates the ferryl hemoglobin species (H-Hb-Fe(IV)=O) plus protein-based radicals (*Hb-Fe(IV)=O). These species induce hemolysis of erythrocytes, polymerization of hemoglobin, and aggregation of the acatalasemic erythrocytes. A mechanism for the onset of Takarara disease is proposed.
Masuoka
Noriyoshi
N
Department of Life Science, Okayama University of Science, 700-0005; Department of Biochemistry, Okayama University Graduate School of Medicine and Dentistry, 700-8558. Electronic address: masuokan@dls.ous.ac.jp.
Sugiyama
Hitoshi
H
Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine and Dentistry, 700-8558.
Ishibashi
Naoki
N
Okayama University Medical School, 700-8558.
Wang
Da-Hong
DH
Department of Public Health, Okayama University Graduate School of Medicine and Dentistry, 700-8558.
Masuoka
Takayoshi
T
Department of Pharmacology, Faculty of Pharmaceutical Science, Okayama University, 700-8530.
Kodama
Hiroyuki
H
Department of Anesthesiology and Critical Care Medicine, Kochi University Medical School, 783-8505.
Nakano
Taku
T
Department of Pharmaceutical Science, University of Toyama, 930-0194, Japan.
D020642:Acatalasia / Q000097:blood* / Q000209:etiology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology*; D006454:Hemoglobins; D006461:Hemolysis / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011108:Polymers
D006454:Hemoglobins; D011108:Polymers; D006861:Hydrogen Peroxide