title: Bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability.
abstract: Five bacterial strains were compared for halogenated compounds conversion in aqueous media. Depending on the strain, the optimal temperature for dehalogenase activity of resting cells varied from 30 to 45 degrees C, while optimal pH raised from 8.4 to 9.0. The most effective dehalogenase activity for 1-chlorobutane conversion was detected with Rhodococcus erythropolis NCIMB13064 and Escherichia coli BL21 (DE3) (DhaA). The presence of 2-chlorobutane or propanal in the aqueous media could inhibit the 1-chlorobutane transformation.
authors.affiliation: Laboratoire de Biotechnologies et de Chimie Bio-organique CNRS FRE 2766, Bâtiment Marie Curie, Université de La Rochelle, Avenue Michel Crépeau 17042 La Rochelle cedex 1, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Erable
authors.forename: Isabelle
authors.initials: I
authors.lastname: Goubet
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lamare
authors.forename: Marie-Dominique
authors.initials: MD
authors.lastname: Legoy
authors.forename: Thierry
authors.initials: T
authors.lastname: Maugard
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors; D002073:Butanes / Q000378:metabolism; D002384:Catalysis; D004785:Environmental Pollutants / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006846:Hydrocarbons, Halogenated / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology; D020578:Sphingomonas / Q000201:enzymology; D013696:Temperature; D020584:Xanthobacter / Q000201:enzymology
chemical_list: D002073:Butanes; D004785:Environmental Pollutants; D006846:Hydrocarbons, Halogenated; D006867:Hydrolases; C046137:haloalkane dehalogenase; C006854:butyl chloride