High resolution imaging of live mitochondria.
Classically, mitochondria have been studied by biochemical, genetic and electron microscopic approaches. In the last two decades, it became evident that mitochondria are highly dynamic organelles that are frequently dividing and fusing, changing size and shape and traveling long distances throughout the life of a cell. The study of the complex structural changes of mitochondria in vivo became possible with the advent of fluorescent labeling techniques in combination with live cell imaging microscopy. This review aims to provide an overview on novel fluorescent markers that are used in combination with mitochondrial fusion assays and various live cell microscopy techniques to study mitochondrial dynamics. In particular, approaches to study the movement of mitochondrial proteins and novel imaging techniques (FRET imaging-, 4Pi- and STED-microscopy) that provide high spatial resolution are considered.
Jakobs
Stefan
S
Max-Planck-Institute for Biophysical Chemistry, Department of NanoBiophotonics, Mitochondrial Structure and Dynamics Group, Am Fassberg 11, 37077 Goettingen, Germany. sjakobs@gwdg.de
D000818:Animals; D036681:Fluorescence Recovery After Photobleaching; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D004718:Saccharomycetales / Q000166:cytology
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins