[Contribution of first polar body analysis to understanding of human aneuploidy mechanism].
In our species, reproduction failure rate is high. Clinical evidence is miscarriage where chromosomal origin was largely involved (66% of cases). The development of assisted reproduction techniques made possible to analyse unfertilized oocytes and preimplantation embryos. The results have shown a high rate of chromosome abnormalities before implantation (50%). Two mechanisms were identified which could generate aneuploidy, the meiosis non-disjunction and the premature separation of sister chromatids (PSSC). A FISH analysis of the first polar body, a cell complementary to the oocyte after meiosis, can be performed before intracytoplasmic sperm injection. We studied 2 distinct populations (patients over 38 years old and patients with recurrent implantation failure) and we confirmed that PSSC is the major mechanism linked to advanced maternal age in human.
Selva
Jacqueline
J
Département de Biologie de la Reproduction, Cytogénétique, Génétique Médicale et Gynécologie Obstétrique, CHI Poissy Saint Germain, 10 rue du Champ Gaillard, 78303 Poissy Cedex.
Bergere
Marianne
M
Molina-Gomes
Denise
D
Hammoud
Ibrahim
I
Lombroso
Raoul
R
Vialard
François
F
D000782:Aneuploidy; D002842:Chromatids / Q000235:genetics; D020090:Chromosome Segregation; D005260:Female; D006801:Humans; D008423:Maternal Age