title: [The impact of rhG-CSF mobilization on migration and adhesive function of CD4+ T cells].
abstract: OBJECTIVE
  To explore the impact of mobilization with recombinant human granulocyte colony stimulated factor (rhG-CSF) on the migration and adhesive function and their related signal mechanism mediated by the CXCR4 and lymphocyte function antigen-1 (LFA-1) molecules on the surfaces of CD4(+) T cells.
  
  
  METHODS
  Before and at day 5 on rhG-CSF mobilization, the expression rates of CXCR4 and LFA-1 (CD11a) on CD4(+) T cells in the peripheral blood were detected by tricolor fluorescence labeling, and the migration and adhesive activities of CD4(+) T cells to stroma cell-derived factor 1 alpha (SDF-1 alpha) and intercellular adhesion molecule-1 (ICAM-1) were also tested.
  
  
  RESULTS
  The expression of CXCR4 on CD4(+) T lymphocytes was (84.58 +/- 20.31)% before mobilization and (81.23 +/- 22.46)% at day 5 on mobilization. The expression of LFA-1 on CD4(+) T lymphocytes before and at day 5 on mobilization was 100%. There was no significant difference in the expression CXCR-4 and LFA-1 on CD4(+) T lymphocytes whether mobilization (P > 0.05). SDF-1 alpha induced 4 hours' CD4(+) T cells migration didn't change markedly before and after mobilization \[(28.5 +/- 10.3)% vs (31.2 +/- 8.9)%\] (P > 0.05). The adhesive activity of CD4(+) T cells to ICAM-1 was decreased from (85.59 +/- 14.21)% to (61.45 +/- 15.07)% after mobilization (P < 0.05).
  
  
  CONCLUSIONS
  The expression of CXCR4 and LFA-1 on CD4(+) T lymphocytes didn't change markedly during rhG-CSF mobilization, but the adhesive activity of CD4(+) T cells to ICAM-1 was frustrated after that.
authors.affiliation: The Hematology Department of PLA General Hospital, Beijing 100853, China.
authors.forename: Wen-rong
authors.initials: WR
authors.lastname: Huang
authors.forename: Li-sheng
authors.initials: LS
authors.lastname: Wang
authors.forename: Xin-li
authors.initials: XL
authors.lastname: Deng
authors.forename: Chun-ji
authors.initials: CJ
authors.lastname: Gao
authors.forename: Zhuo-zhuang
authors.initials: ZZ
authors.lastname: Lu
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Hai-feng
authors.initials: HF
authors.lastname: Duan
authors.forename: Wan-ming
authors.initials: WM
authors.lastname: Da
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000502:physiology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D011994:Recombinant Proteins
chemical_list: D054377:Chemokine CXCL12; D016169:Lymphocyte Function-Associated Antigen-1; D019718:Receptors, CXCR4; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D016179:Granulocyte Colony-Stimulating Factor