title: Chromatin assembly of DNA templates microinjected into Xenopus oocytes.
abstract: The packaging of deoxyribonucleic acid (DNA) into chromatin within the eukaryotic nucleus can affect processes such as DNA replication, transcription, recombination, and repair. Therefore, studies aimed at understanding at the molecular level how these processes are operating have to take into account the chromatin context. We present a method to assemble DNA into chromatin by nuclear microinjection into Xenopus oocytes. This method allows in vivo chromatin formation in a nuclear environment. We provide the experimental procedures for oocyte preparation, DNA injection, and analysis of the assembled chromatin.
authors.affiliation: Research Section, Institute Curie, UMR218 du Centre National de la Recherche Scientifique, Paris, France.
authors.forename: Danièle
authors.initials: D
authors.lastname: Roche
authors.forename: Geneviève
authors.initials: G
authors.lastname: Almouzni
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Quivy
mesh_terms: D000818:Animals; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D004270:DNA, Circular / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D005260:Female; D008845:Microinjections / Q000379:methods*; D009865:Oocytes / Q000378:metabolism*; D013698:Templates, Genetic; D014981:Xenopus / Q000235:genetics
chemical_list: D004270:DNA, Circular; D004277:DNA, Single-Stranded