title: Mobilisation into cotton and spread of a recombinant cotton leaf curl disease satellite.
abstract: Analysis of a DNA beta satellite associated with a recently identified cotton leaf curl disease (CLCuD) strain indicated it to be recombinant, with most of the molecule originating from CLCuD DNA beta but with some sequence from a satellite isolated from tomato. Analysis of both archival (pre 2001) and recent cotton samples, shows the recombinant satellite is confined to a small area but was not present in cotton prior to 2001. This indicates that the recombinant DNA beta was recently mobilized into cotton, likely from tomato, and that recombination plays a role in the evolution of these satellites.
authors.affiliation: National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. imran@nibge.org
authors.forename: I
authors.initials: I
authors.lastname: Amin
authors.forename: S
authors.initials: S
authors.lastname: Mansoor
authors.forename: L
authors.initials: L
authors.lastname: Amrao
authors.forename: M
authors.initials: M
authors.lastname: Hussain
authors.forename: S
authors.initials: S
authors.lastname: Irum
authors.forename: Y
authors.initials: Y
authors.lastname: Zafar
authors.forename: S E
authors.initials: SE
authors.lastname: Bull
authors.forename: R W
authors.initials: RW
authors.lastname: Briddon
mesh_terms: D001483:Base Sequence; D005075:Biological Evolution; D004276:DNA, Satellite / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D017791:Geminiviridae / Q000235:genetics* / Q000302:isolation & purification; D003368:Gossypium / Q000821:virology*; D008969:Molecular Sequence Data; D010154:Pakistan; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D011995:Recombination, Genetic; D016415:Sequence Alignment
chemical_list: D004276:DNA, Satellite; D004279:DNA, Viral