The in vitro antiviral activity of an aliphatic nitro compound from Heteropteris aphrodisiaca.
We investigated the antiviral activity of an aliphatic nitro compound (NC) isolated from Heteropteris aphrodisiaca O. Mach. (Malpighiaceae), a Brazilian medicinal plant. The NC was tested for its antiviral activity against poliovirus type 1 (PV-1) and bovine herpes virus type 1 (BHV-1) by plaque reduction assay in cell culture. The NC showed a moderate antiviral activity against PV-1 and BHV-1 in HEp-2 cells, and the 50% inhibitory concentration (IC50) were 22.01 microg/ml (selectivity index (SI)=2.83) and 21.10 microg/ml (SI=2.95), respectively. At the highest concentration of the drug (40 microg/ml) a reduction of approximately 80% in plaque assay was observed for both viruses. The treatment of cells or virus prior to infection did not inhibit the replication of virus strains.
Melo
Fernando L
FL
Departamento de Microbiologia/CCB, Universidade Estadual de Londrina, Caixa Postal 6001, CEP 86051-990 Londrina-Paraná, Brazil.
Benati
Fabricio J
FJ
Roman
Walter Antonio
WA
de Mello
João Carlos Palazzo
JC
Nozawa
Carlos
C
Linhares
Rosa Elisa Carvalho
RE
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001938:Brazil; D002417:Cattle; D045744:Cell Line, Tumor; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D007242:Herpesvirus 1, Bovine / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D009574:Nitro Compounds / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D017955:Poliovirus / Q000187:drug effects*; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D009574:Nitro Compounds; D010936:Plant Extracts