title: A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure.
abstract: A novel vaccine against porcine parvovirus (PPV), composed of recombinant virus-like particles (PPV-VLPs) produced with the baculovirus expression vector system (BEVS) at industrial scale, was tested for its immunogenicity and protective potency. A formulation of submicrogram amounts of PPV-VLPs in a water-in-mineral oil adjuvant evoked high serum antibody titres in both guinea pigs, used as reference model, and target species, pigs. A single immunisation with 0.7microg of this antigen yielded complete foetal protection against PPV infection after challenge with a virulent strain of this virus. Furthermore, also in the presence of mild adjuvants the protective action of these PPV-VLPs is excellent. This recombinant subunit vaccine overcomes some of the drawbacks of classical PPV vaccines.
authors.affiliation: Animal Sciences Group (ASG, Previously ID-Lelystad), P.O. Box 65, 8200 AB Lelystad, The Netherlands.
authors.forename: Adriaan F G
authors.initials: AF
authors.lastname: Antonis
authors.forename: Christianne J M
authors.initials: CJ
authors.lastname: Bruschke
authors.forename: Paloma
authors.initials: P
authors.lastname: Rueda
authors.forename: Luis
authors.initials: L
authors.lastname: Maranga
authors.forename: J Ignacio
authors.initials: JI
authors.lastname: Casal
authors.forename: Carmen
authors.initials: C
authors.lastname: Vela
authors.forename: Luuk A Th
authors.initials: LA
authors.lastname: Hilgers
authors.forename: Peter B G M
authors.initials: PB
authors.lastname: Belt
authors.forename: Klaas
authors.initials: K
authors.lastname: Weerdmeester
authors.forename: Manuel J T
authors.initials: MJ
authors.lastname: Carrondo
authors.forename: Jan P M
authors.initials: JP
authors.lastname: Langeveld
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D010322:Parvoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D028323:Parvovirus, Porcine / Q000276:immunology*; D011247:Pregnancy; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014765:Viral Vaccines / Q000009:adverse effects / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D014765:Viral Vaccines