Centrifugal extraction of plasma from whole blood on a rotating disk.
We present a centrifugal process for the extraction of plasma from sediment by a decanting structure, terminating with metered plasma which is readily available for subsequent on-disk processing. Our technique supplies 2 microl plasma from 5 microl of whole blood at moderate spinning frequencies of 40 Hz within 20 s, only. The residual cell concentration in the purified plasma amounts to less than 0.11%, independent of the frequency of rotation. A capillary duct connects the extracted plasma to subsequent on-disk processing units.
Haeberle
Stefan
S
Laboratory for MEMS Applications, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany. haeberle@imtek.de
Brenner
Thilo
T
Zengerle
Roland
R
Ducrée
Jens
J
D001769:Blood; D001799:Blood Sedimentation; D002498:Centrifugation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D010949:Plasma