Regulation of beta1 integrin expression in endothelial cells by chimeric tRNA(Val) ribozyme.
To downregulate expression of the beta1 integrin subunit in endothelial cells, plasmid encoding the ribozyme cassette containing hammerhead ribozyme flanked at the 5' terminus by tRNA(Val) and at the 3' terminus by constitutive transport element sequences was constructed. When used to transfect immortalized human endothelial cell line EA.hy 926, it selectively blocked the synthesis of the beta1 integrin subunit and thus inhibited migration and proliferation of the cells. Thus, this construct may be a valuable tool to control the proangiogenic phenotype of stimulated endothelial cells.
Papiewska-Pajak
Izabela
I
Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland. ibizka@zdn.am.lodz.pl
Antoszczyk
Sławomir
S
D002460:Cell Line; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics*; D008957:Models, Genetic; D016337:RNA, Catalytic / Q000235:genetics*; D012366:RNA, Transfer, Val / Q000235:genetics*; D013997:Time Factors
D019012:Integrin beta1; D016337:RNA, Catalytic; D012366:RNA, Transfer, Val