[Effects of PprI and RecX on antioxidant activity of Deinococcus radiodurans].
Effects of mutations of Pprl (Dr0167) and RecX (Dr1310), which are relative to radioresistance, on reactive oxygen species scavenging activities in Deinococcus radiodurans were investigated using gene mutation, chemiluminescence measurement and enzyme activity analysis. Their possible regulating functions on the activities of antioxidant enzymes was evaluated. Results show that mutant that lacks PprI is remarkably sensitive to reactive oxygen species and its enzyme activities of catalase and superoxide dismutase decrease significantly. On the other hand, RecX has a "negative" effect on reactive oxygen species scavenging activities of this bacterium, i.e., mutation of recX enhances the scavenging activities on reactive oxygen species, and the enzyme activities of catalase and superoxide dismutase in mutant that lacks RecX are significantly increased. These results indicate that these two genes are relative to the regulation of antioxidant system of this bacterium. It presents some idea to the further investigation on the antioxidant mechanism of this bacterium.
Tian
Bing
B
Institute of Nuclear Agricultral Sciences, Zhejiang University, Key Lab of Nuclear-Agricultural Sciences of Ministry of Agriculture, Hangzhou 310029, China. tianbing@zju.edu.cn
Zhang
Shao-Wen
SW
Xu
Zhen-Jian
ZJ
Sheng
Duo-Hong
DH
Hua
Yue-Jin
YJ
D000975:Antioxidants / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D034301:Deinococcus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D009154:Mutation; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; C430523:RecX protein, Xanthomonas campestris; D002374:Catalase; D013482:Superoxide Dismutase