Expression of HLA-G in cancer bladder.
This study comprised 42 patients with cancer bladder, who underwent radical cystectomy. The aim of work is to determine if HLA-G is expressed on tumor cells, derived from cancer bladder. We studied HLA-G-mRNA expression using RT-PCR and HLA-G cell surface expression by immunohistochemistry (IHC) staining technique. HLA-G was expressed in 28.6 % of cancer cases as determined by PCR and on 16.7% of cases determined by IHC staining. The sensitivity, specificity and accuracy of IHC were 58.3%, 96% and 81.7% respectively as compared to PCR results. There was a highly significant increase in the expression of HLA-G on cancer bladder cases with metastatic prostate infiltration (P= 0.021). It is concluded that HLA-G is ectopically expressed on cancer bladder malignant cells both at molecular and protein levels. However, it is not significantly associated with histologic type, tumor grade, stage, T category, schistosomiasis and lymph node involvement.
El-Chennawi
Farha A
FA
Clinical Immunology Unit, Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Auf
Fatma A
FA
El-Diasty
Amany M
AM
El-Daim
Mohamed Abd
MA
El-Sherbiny
Sherif M
SM
Ali
Ayman
A
El-Baz
Mohamoud
M
El-Hameed
Mohammed Abd
MA
Paul
Pascal
P
Ibrahim
El-Cherif
EC
Carosella
Edgardo
E
Ghoneim
Mohammed A
MA
D000328:Adult; D000368:Aged; D015653:Cystectomy; D005260:Female; D006680:HLA Antigens / Q000096:biosynthesis / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000556:secondary; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I