The usefulness of CD71 expression by flow cytometry for differentiating indolent from aggressive CD10+ B-cell lymphomas.
We studied 34 low- and 30 high-grade CD10+ B-cell lymphomas. Forward light scatter (FSC) and CD71 fluorescence intensity (CD71i) of tumor cells were measured and normalized by corresponding values for resting T cells. Significant differences in CD71i values between low- and high-grade lymphomas were observed by the Mann-Whitney U test (P < .001) and receiver operating characteristic (ROC) curve analysis (P < .001). FSC was not significantly different between low- and high-grade lymphomas; the area under the ROC curve was less than that for CD71i. Neither FSC nor CD71i significantly differentiated follicular lymphoma (FL) grades. A comparison of all FLs (grades 1-3) and non-FL high-grade lymphomas (Burkitt lymphoma [BL] and large B-cell lymphoma [LBCL]) showed significant differences in CD71i (P < .001) and FSC (P = .021). Differences were significant in CD71i and FSC between FL and LBCL (P < .001) but not between FL and BL. CD71i is more potent than FSC for distinguishing CD10+ low- from high-grade lymphomas and FL from non-FL high-grade lymphomas. Sensitivity and specificity are limited owing to inability to identify FL3. In ROC analysis, a high value for CD71i can identify BL and LBCL with a high degree of certainty.
Wu
Julie M
JM
Department of Pathology, Johns Hopkins Medical, Institutions, Baltimore, MD, USA.
Borowitz
Michael J
MJ
Weir
Edward G
EG
D015703:Antigens, CD / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D003937:Diagnosis, Differential; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D008027:Light; D016393:Lymphoma, B-Cell / Q000145:classification / Q000378:metabolism / Q000473:pathology*; D015260:Neprilysin / Q000378:metabolism; D012372:ROC Curve; D011990:Receptors, Transferrin / Q000378:metabolism*; D012189:Retrospective Studies; D012542:Scattering, Radiation
D015703:Antigens, CD; D014408:Biomarkers, Tumor; C068126:CD71 antigen; D011990:Receptors, Transferrin; D015260:Neprilysin