title: [Studies on polyploid induction of Phellodendron chinense].
abstract: OBJECTIVE
  This paper aimed at improving existing breeds of Phellodendron chinense and enriching the resources of heredity breeding.
  
  
  METHODS
  The polyploid of P. chinense was obtained using calli, seeds and buds, which were treated with colchicines solution.
  
  
  RESULTS
  The polyploid of P. chinense was obtained using calli with stem treated with 0.2% colchicines medium, the inducing rate reached 12.45%. Compared with the morphology and chromosomal number the polyploid induction was proved to be effective.
  
  
  CONCLUSIONS
  It was an effective biotechnological way to use colchicines to induce polyploid on the different explants of P. chinense.
authors.affiliation: Department of Biological Engineering, Chengdu University, Chengdu 610160, China. tlfor@hotmail.com
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Wang
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D003078:Colchicine / Q000494:pharmacology*; D032102:Phellodendron / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D018520:Plant Shoots / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D011123:Polyploidy; D012639:Seeds / Q000235:genetics / Q000254:growth & development
chemical_list: D003078:Colchicine