title: Evaluation of reproductive potential after intracytoplasmic sperm injection of varied human semen tested by antiacrosomal antibodies.
abstract: OBJECTIVE
  To determine whether varied human spermatozoa, as detected with monoclonal antibodies against acrosomal proteins, have an influence on fertilization, transfer, pregnancy, and implantation rates when intracytoplasmic sperm injection is used.
  
  
  METHODS
  A retrospective study.
  
  
  METHODS
  A private IVF center and academic research laboratory.
  
  
  METHODS
  One thousand two hundred forty men participating in the intracytoplasmic sperm injection program.
  
  
  METHODS
  Sperm were divided into seven groups: oligozoospermia, oligoasthenozoospermia, and oligoasthenoteratozoospermia and fresh and frozen-thawed epididymal and fresh and frozen-thawed testicular sperm. Fertilization, transfer, pregnancy, and implantation rates were recorded in each category. Sperm were tested with antibodies for detection of the of the sperm acrosome.
  
  
  METHODS
  Fertilization, transfer, pregnancy and implantation rates, and percentage of acrosome-reacted cells.
  
  
  RESULTS
  The fertilization rate and statistical evaluation showed differences between morphologically normal and pathological sperm and other groups. The freezing-thawing procedure had no influence on the fertilization of testicular sperm, but epididymal frozen-thawed sperm had a higher fertilization rate. Immunofluorescence proved decreasing sperm quality in all groups compared with the control group. This difference is not manifested in other parameters (transfer, pregnancy, implantation rates).
  
  
  CONCLUSIONS
  The spermatozoa with varied semen characteristics and good quality, also detected with specific antibodies, gave the best fertilization rates. The paternal effect is not proved in other parameters.
authors.affiliation: Iscare IVF Ltd, Prague, Czech Republic.
authors.forename: Olina
authors.initials: O
authors.lastname: Tepla
authors.forename: Jana
authors.initials: J
authors.lastname: Peknicova
authors.forename: Karel
authors.initials: K
authors.lastname: Koci
authors.forename: Josef
authors.initials: J
authors.lastname: Mika
authors.forename: Milan
authors.initials: M
authors.lastname: Mrazek
authors.forename: Fatima
authors.initials: F
authors.lastname: Elzeinova
mesh_terms: D000177:Acrosome / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000032:analysis; D002478:Cells, Cultured; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007248:Infertility, Male / Q000453:epidemiology* / Q000276:immunology / Q000628:therapy*; D008297:Male; D017063:Outcome Assessment, Health Care / Q000379:methods*; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D012661:Semen / Q000166:cytology* / Q000276:immunology*; D020554:Sperm Injections, Intracytoplasmic / Q000706:statistics & numerical data*; D016896:Treatment Outcome
chemical_list: D000936:Antigen-Antibody Complex