[Cloning and characterization of two novel alternatively spliced transcripts of ZNF268].
ZNF268 gene encodes a typical KRAB-containing zinc finger protein, which has an amino-terminal KRAB domain and 24 carboxyl-terminal C2H2 zinc finger motifs. There is evidence that ZNF268 is expressed in human early embryos, and that it played a role in the development of human fetal liver. In this study, we use immunohistochemistry to show that ZNF268 was also expressed in hematopoietic stem cells in 3-5 week-old human embryos. ZNF268 expression was also detected in the blood cells from 7 healthy adult donors by RT-PCR. Furthermore, 4 alternative transcripts were detected in the 7 samples. By cloning and sequencing, two novel transcripts, ZNF268c and ZNF268d were identified. We predict that there is association between ZNF268 and development of hematopoietic cells.
Sun
Chong
C
College of Life Sciences, Wuhan University, Wuhan 430072, China. lunnarnight@163.com
Zhao
Zhou-Zhou
ZZ
Gao
Li
L
Sun
Yan
Y
Shao
Huan-Jie
HJ
Li
Wen-Xin
WX
D017398:Alternative Splicing; D001773:Blood Cells / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D008297:Male; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C482295:ZNF268 protein, human