Comparison of cell populations derived from canine olfactory bulb and olfactory mucosal cultures.
OBJECTIVE
To evaluate the numbers and proportions of olfactory ensheathing cells (OECs) in cell cultures derived from the olfactory bulb (OB) and olfactory mucosa of dogs.


METHODS
7 dogs.


METHODS
OB tissue and olfactory mucosa from the nasal cavity and frontal sinus were obtained from euthanatized dogs and prepared for cell culture. At 7, 14, and 21 days of culture in vitro, numbers and proportions of OECs, astrocytes, and fibroblasts were determined via immunocytochemistry. Antibody against the low-affinity nerve growth factor receptor p75 was used to identify OECs, antibody against glial fibrillary acidic protein was used to identify astrocytes, and antibody against fibronectin was used to identify fibroblasts.


RESULTS
Cultured OECs derived from the olfactory mucosa of the nasal cavity and frontal sinus had similar characteristics. However, whereas OECs in the OB cell cultures constituted approximately 50% of the cells at 7 days and approximately 75% at 21 days the proportion of OECs in cultures derived from both mucosal types was much lower, with approximately 40% OECs at 7 days and approximately 25% at 21 days. Analysis of OEC numbers revealed that these changes were accompanied by corresponding decreases and increases in the population of cells with fibronectin receptors.


CONCLUSIONS
Although olfactory mucosal cell cultures yielded a sufficient number of OECs for spinal cord transplantation procedures in dogs, modification of culture conditions would be required to ensure that the derived cell population contained a sufficient proportion of OECs.
Ito
Daisuke
D
Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK.
Ibanez
Chrystelle
C
Ogawa
Hiroyuki
H
Franklin
Robin J M
RJ
Jeffery
Nick D
ND
D000818:Animals; D018929:Cell Culture Techniques; D004285:Dogs / Q000502:physiology*; D009297:Nasal Mucosa / Q000166:cytology*; D009830:Olfactory Bulb / Q000166:cytology*