title: Embryo transfer as a means of controlling the transmission of viral infections. I. The in vitro exposure of preimplantation bovine embryos to akabane, bluetongue and bovine viral diarrhea viruses.
abstract: As part of a program to study the feasibility of using embryo transfer to control disease, initial experiments were undertaken to determine the virus susceptibility of early embryos. Two hundred and ninety-three preimplantation bovine embryos (16-cell to blastocyst stage) were exposed to either akabane virus (AV), bluetongue virus (BTV) or bovine viral diarrhea virus (BVDV). Two hundred and thirty-seven of these embryos were then cultured for 24-48 hours in order to determine whether the virus had any effect on embryonic development and to allow viral replication to occur. No infectious virus was isolated from any of the embryos and the in vitro development of virus exposed embryos proceeded normally. In addition, twenty-nine eggs/embryos isolated from donors that were seropositive to BVDV were found to be uninfected with this virus.
authors.affiliation: Agriculture Canada, Animal Diseases Research Institute, NEPEAN P.O. Box 11300, Station "H", Nepean, Ontario, Canada, K2H 8P9.
authors.forename: E L
authors.initials: EL
authors.lastname: Singh
authors.forename: M D
authors.initials: MD
authors.lastname: Eaglesome
authors.forename: F C
authors.initials: FC
authors.lastname: Thomas
authors.forename: G
authors.initials: G
authors.lastname: Papp-Vid
authors.forename: W C
authors.initials: WC
authors.lastname: Hare