Isolation of functional ingredients from rosemary by preparative-supercritical fluid chromatography (Prep-SFC).
A supercritical fluid extract of rosemary has been fractionated under supercritical conditions by using a preparative-SFC system. In this work, the optimum conditions have been evaluated to achieve a selective isolation of the compounds responsible for both, antioxidant and antimicrobial activities. A 25 cm x 10 mm i.d. LC-Diol packed column (dp=5 microm) has been used and the separation took place at 80 degrees C of column temperature, 130 bar of pressure, and 10% of ethanol as modifier of the mobile phase (CO(2)). Two cyclones were employed to collect the fractions which were subsequently characterized by HPLC-DAD, GC, and in vitro antioxidant and antimicrobial assays. By a careful selection of the separation conditions it is possible to obtain two different fractions, one enriched with antioxidant and antimicrobial compounds (with an improvement of about 20% and 40% of antioxidant and antimicrobial activity, respectively, compared to the original extract) collected in cyclone 2 and with no residual rosemary aroma and another one containing the essential oil.
Ramírez
Pilar
P
Sección Departamental Ciencias de la Alimentación, Unidad Asociada al CSIC, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
García-Risco
Mónica R
MR
Santoyo
Susana
S
Señoráns
F Javier
FJ
Ibáñez
Elena
E
Reglero
Guillermo
G
D045784:Abietanes / Q000032:analysis; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry; D001713:Biphenyl Compounds; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements / Q000032:analysis; D005504:Food Analysis; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D029794:Ledum / Q000737:chemistry*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D009812:Odorants; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects
D045784:Abietanes; D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl; C018381:salvin