title: Immunologic and clinical responses after vaccinations with peptide-pulsed dendritic cells in metastatic renal cancer patients.
abstract: A phase I trial was conducted to evaluate the feasibility, safety, and efficacy of a dendritic cell-based vaccination in patients with metastatic renal cell carcinoma (RCC). Autologous mature dendritic cells derived from peripheral blood monocytes were pulsed with the HLA-A2-binding MUC1 peptides (M1.1 and M1.2). For the activation of CD4(+) T-helper lymphocytes, dendritic cells were further incubated with the PAN-DR-binding peptide PADRE. Dendritic cell vaccinations were done s.c. every 2 weeks for four times and repeated monthly until tumor progression. After five dendritic cell injections, patients additionally received three injections weekly of low-dose interleukin-2 (1 million IE/m(2)). The induction of vaccine-induced T-cell responses was monitored using enzyme-linked immunospot and Cr release assays. Twenty patients were included. The treatment was well tolerated with no severe side effects. In six patients, regression of the metastatic sites was induced after vaccinations with three patients achieving an objective response (one complete response, two partial responses, two mixed responses, and one stable disease). Additional four patients were stable during the treatment for up to 14 months. MUC1 peptide-specific T-cell responses in vivo were detected in the peripheral blood mononuclear cells of the six patients with objective responses. Interestingly, in patients responding to the treatment, T-cell responses to antigens not used for vaccinations, such as adipophilin, telomerase, or oncofetal antigen, could be detected, indicating that epitope spreading might occur. This study shows that MUC1 peptide-pulsed dendritic cells can induce clinical and immunologic responses in patients with metastatic RCC.
authors.affiliation: Department of Hematology, University of Tübingen, Tübingen, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Wierecky
authors.forename: Martin R
authors.initials: MR
authors.lastname: Müller
authors.forename: Stefan
authors.initials: S
authors.lastname: Wirths
authors.forename: Edith
authors.initials: E
authors.lastname: Halder-Oehler
authors.forename: Daniela
authors.initials: D
authors.lastname: Dörfel
authors.forename: Susanne M
authors.initials: SM
authors.lastname: Schmidt
authors.forename: Maik
authors.initials: M
authors.lastname: Häntschel
authors.forename: Wolfram
authors.initials: W
authors.lastname: Brugger
authors.forename: Stephen
authors.initials: S
authors.lastname: Schröder
authors.forename: Marius S
authors.initials: MS
authors.lastname: Horger
authors.forename: Lothar
authors.initials: L
authors.lastname: Kanz
authors.forename: Peter
authors.initials: P
authors.lastname: Brossart
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology*; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000628:therapy*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007680:Kidney Neoplasms / Q000276:immunology / Q000628:therapy*; D008213:Lymphocyte Activation; D017780:Malaria Vaccines / Q000276:immunology; D008297:Male; D008875:Middle Aged; D018396:Mucin-1; D009077:Mucins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D015789:HLA-A2 Antigen; C060192:MUC1 protein, human; D017780:Malaria Vaccines; D018396:Mucin-1; D009077:Mucins; C401223:PADRE 45