title: [Effect of Herba Epimedii flavone on the osteoblasts metabolism in vitro].
abstract: OBJECTIVE
  To explore the effect of Herba Epimedii flavone (HEF) on the osteoblast metabolism in vitro.
  
  
  METHODS
  Osteoblast were obtained from new born rat calvaria by digestive enzymes. MTF, PNPP and RT-PCR were used to observe the proliferation, activity of ALP and mRNA expression of OPG and RANKL of cultured osteoblasts in vitro.
  
  
  RESULTS
  It was found that HEF had the effect on stimulating cell proliferation, activity of ALP and the mRNA expression of OPG of cultured osteoblasts (P < 0.01, P < 0.05).
  
  
  CONCLUSIONS
  HEF can promote the proliferation, the differentiation and the expression of OPG mRNA of the osteoblasts cultured in vitro.
authors.affiliation: Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. liuyiheng-xjtu@sohu.com
authors.forename: Yi-Heng
authors.initials: YH
authors.lastname: Liu
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Zhang
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Zang
authors.forename: Jun-Chang
authors.initials: JC
authors.lastname: Cheng
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D034181:Epimedium / Q000737:chemistry; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D053244:Osteoprotegerin; D010946:Plants, Medicinal / Q000737:chemistry; D053245:RANK Ligand; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D047309:Flavones; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506032:Tnfrsf11b protein, rat; D000469:Alkaline Phosphatase