BCL6 alternative breakpoint region break and homozygous deletion of 17q24 in the nodular lymphocyte predominance type of Hodgkin's lymphoma-derived cell line DEV.
DEV is the only cell line derived from nodular lymphocyte predominance type of Hodgkin's lymphoma (NLPHL); however, a comprehensive report about the genetic and immunophenotypic profile of this unique cell line is lacking. We analyzed DEV with respect to immunophenotype and genetic aberrations. The immunostaining revealed positivity for CD45, CD20, CD22, CD79a, IgA2, CD80, CD86, CD74, and BCL6. Cytogenetically, DEV has complex chromosome 3 translocations involving chromosomes 7, 14, and 22. A detailed analysis of the 3q27 breakpoint of the der(3)t(3;14)(p14;q32)t(3;22)(q27;q11.2) revealed a break in the BCL6 alternative breakpoint region. Using array comparative genomic hybridization, a 3-megabase homozygous deletion at 17q24.1-24.2 was identified. Fluorescence in situ hybridization indicated the presence of 2 chromosome 17 homologues, each of which carried a small interstitial deletion. Eight microsatellite markers flanking the homozygously deleted region all showed a homozygous pattern suggesting loss of one of the parental alleles. D17S1809 and D17S1816 could not be amplified using DEV DNA, in keeping with a location within the homozygously deleted segment. In conclusion, DEV has an immunophenotype that is consistent with the neoplastic cells of NLPHL cases, the lymphocytic and histiocytic cells. We demonstrated involvement of the BCL6 gene based on the presence of a breakpoint in the alternative breakpoint region and nuclear staining for BCL6 protein and identified a homozygously deleted region at 17q24.
Atayar
Ciğdem
C
Department of Pathology and Laboratory Medicine, University Medical Centre Groningen and University of Groningen 9700 RB, The Netherlands.
Kok
Klaas
K
Kluiver
Joost
J
Bosga
Anneke
A
van den Berg
Eva
E
van der Vlies
Pieter
P
Blokzijl
Tjasso
T
Harms
Geert
G
Davelaar
Inge
I
Sikkema-Raddatz
Birgit
B
Martin-Subero
Jose Ignacio
JI
Siebert
Reiner
R
Poppema
Sibrand
S
van den Berg
Anke
A
D045744:Cell Line, Tumor; D019457:Chromosome Breakage; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D020732:Cytogenetic Analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D005819:Genetic Markers; D006689:Hodgkin Disease / Q000235:genetics* / Q000473:pathology; D006720:Homozygote; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D051560:Proto-Oncogene Proteins c-bcl-6
C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D051560:Proto-Oncogene Proteins c-bcl-6