title: Bioactive secolignans from Peperomia dindygulensis.
abstract: Thirteen secolignans, including eight new ones (1-8), were isolated from the EtOAc extract of Peperomia dindygulensis. The structures were mainly elucidated by 1D and 2D NMR and MS experiments, the relative configurations were determined by NOE correlations, and the absolute configurations were established by the optical rotations and CD spectra. Cytotoxicity and MDR (multidrug resistance) reversal activity of the isolated compounds were examined. Compounds 6 and 7, peperomins B (10) and E (12), showed moderate to strong growth inhibitory activity against a malignant lung tumor cell (VA-13) with IC(50) values of 15.2, 13.5, 13.9, and 1.93 microM, respectively, and also inhibited the growth of a normal lung fibroblast cell (WI-38) at the same levels. Compound 7 and peperomin E (12) exhibited inhibitory activity against a liver tumor cell (HepG2) with IC(50) values of 22.3 and 12.1 microM. Compounds 5 and 7 and peperomins A, B, C, and E (9-12) enhanced calcein accumulation in MDR 2780 cells at 25 microg/mL. Compounds 2, 3, 7, and peperomin E (12) showed inhibitory activity on induction of the intercellular adhesion molecule-1 (ICAM-1).
authors.affiliation: Graduate School of Science and Technology, Niigata University, Ikarashi, Niigata 950-2181, Japan.
authors.forename: Jian-Lin
authors.initials: JL
authors.lastname: Wu
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Sakai
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Mitsui
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Ogura
authors.forename: Takao
authors.initials: T
authors.lastname: Kataoka
authors.forename: Seiko
authors.initials: S
authors.lastname: Oka
authors.forename: Miwa
authors.initials: M
authors.lastname: Kiuchi
authors.forename: Akira
authors.initials: A
authors.lastname: Tomida
authors.forename: Takashi
authors.initials: T
authors.lastname: Turuo
authors.forename: Minjie
authors.initials: M
authors.lastname: Li
authors.forename: Wanxia
authors.initials: W
authors.lastname: Tang
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ando
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018432:Drug Resistance, Multiple; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D036941:Peperomia / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D017705:Lignans; D018799:Intercellular Adhesion Molecule-1