title: MultiPrimer: software for multiplex primer design.
abstract: This study presents a novel application, MultiPrimer, to assist users to design multiplex PCR primers. MultiPrimer adopts an algorithm based on a genetic algorithm and can efficiently solve the multi-objective optimisation problem. A match pattern model is proposed to speed up examination of the specificity constraint. MultiPrimer is able to find a set of primer pairs simultaneously for a set of gene family sequences and multiple targets for a single cDNA sequence. A public version of the MultiPrimer software is available from http://edith.cse.nsysu.edu.tw/new/software/MultiPrimer.htm.
authors.affiliation: Department of Computer Science and Engineering, National Sun Yat-Sen University, Kaohsiung, Taiwan. cnlee@mail.cse.nsysu.edu.tw
authors.forename: Chungnan
authors.initials: C
authors.lastname: Lee
authors.forename: Jain-Shing
authors.initials: JS
authors.lastname: Wu
authors.forename: Yow-Ling
authors.initials: YL
authors.lastname: Shiue
authors.forename: Hong-Long
authors.initials: HL
authors.lastname: Liang
mesh_terms: D000465:Algorithms; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D017931:DNA Primers / Q000737:chemistry*; D018076:DNA, Complementary / Q000378:metabolism; D023281:Genomics / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D014584:User-Computer Interface
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary