title: Selective portal clamping to minimize hepatic ischaemia-reperfusion damage and avoid accelerated outgrowth of experimental colorectal liver metastases.
abstract: BACKGROUND
  Temporary vascular clamping during local ablation for colorectal liver metastases increases destruction volumes. However, it also causes ischaemia-reperfusion (IR) injury to the liver parenchyma and accelerates the outgrowth of microscopic tumour deposits. The aim of this study was to investigate the effects of selective portal clamping on hepatocellular damage and tumour growth.
  
  
  METHODS
  Mice carrying pre-established hepatic colorectal micrometastases underwent either simultaneous clamping of both the portal vein and the hepatic artery or selective clamping of the portal vein to the median and left liver lobes for 45 min. Sham-operated mice served as controls. Hepatic injury and tumour growth were assessed over time.
  
  
  RESULTS
  Standard inflow occlusion resulted in a rise in liver enzymes, a local inflammatory response and hepatocellular necrosis. The outgrowth of pre-established micrometastases was accelerated three- to fourfold in clamped compared with non-clamped liver lobes (27.4 versus 7.8 per cent, P < 0.010). Conversely, selective portal clamping induced minimal liver injury, tissue inflammation or hepatocellular necrosis, and completely stopped the accelerated outgrowth of micrometastases.
  
  
  CONCLUSIONS
  Selective portal clamping does not induce liver tissue damage or accelerate micrometastasis outgrowth and may therefore be the preferable clamping method during local ablative treatment of hepatic metastases.
authors.affiliation: Department of Surgery, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: J D W
authors.initials: JD
authors.lastname: van der Bilt
authors.forename: O
authors.initials: O
authors.lastname: Kranenburg
authors.forename: A
authors.initials: A
authors.lastname: Verheem
authors.forename: R
authors.initials: R
authors.lastname: van Hillegersberg
authors.forename: I H M
authors.initials: IH
authors.lastname: Borel Rinkes
mesh_terms: D000818:Animals; D015179:Colorectal Neoplasms; D003250:Constriction; D006498:Hepatectomy / Q000379:methods*; D008113:Liver Neoplasms / Q000098:blood supply / Q000556:secondary / Q000601:surgery*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015427:Reperfusion Injury / Q000517:prevention & control*