title: [Expression of Nestin, CK19, insulin, glucagon and somatostatin in embryonic pancreas].
abstract: OBJECTIVE
  To investigate the expression of Nestin, CK19, insulin, glucagon and somatostatin in the development of embryonic pancreas.
  
  
  METHODS
  Sample collection was conducted in accordance to the principle of informed consent. Immunohistochemical SABC method and LAB-SA for double satining were used to study the expression of the molecules in human embryonic pancreas.
  
  
  RESULTS
  (1) There are few nestin-positive cells in the stroma of the embryonic pancreas. CK19 is consistently expressed in the epithelial cells of pancreatic ducts. (2) From 7 weeks of gestation, pancreatic duct epithelial cells begin to differentiate into insulin, glucagon and somatostatin-positive cells.
  
  
  CONCLUSIONS
  Nestin-positive pancrease stem cells are in the stroma of human embryonic pancrease. Pancreatic duct epithelial cells differentiate into islet cells secreting insulin at the early stage of embryonic development.
authors.affiliation: Department of Anatomy, Preclinical and Forensic College of Sichuan University, Chengdu 610041, China. kming1965@163.com
authors.forename: Kai-ming
authors.initials: KM
authors.lastname: Yan
authors.forename: Ai-dong
authors.initials: AD
authors.lastname: Li
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Mei
authors.forename: Hong-ying
authors.initials: HY
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005934:Glucagon / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D053539:Keratin-19 / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D010179:Pancreas / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D011247:Pregnancy; D013004:Somatostatin / Q000378:metabolism*
chemical_list: D007328:Insulin; D007381:Intermediate Filament Proteins; D053539:Keratin-19; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D013004:Somatostatin; D005934:Glucagon