Monitoring a complex medium fermentation with sample-sample two-dimensional FT-NIR correlation spectroscopy.
The possibility of monitoring state transitions during the time course of a fermentation through the analysis of the on-line collected near-infrared (NIR) spectra with sample-sample two-dimensional correlation spectroscopy (SS-2DCoS) was investigated. SS-2DCoS has proved to be useful for extracting process information directly from the spectra collected on-line. The complexity of the system studied prevented the extraction of concentration profiles, but nevertheless, the application of SS-2DCoS enables the identification of fermentation state transitions due to metabolic and morphological changes of the microorganism.
Ferreira
Ana P
AP
Centre for Biological and Chemical Engineering, IST, Technical University of Lisbon, Av. Rovisco Pais, P-1049-001 Lisbon, Portugal.
Menezes
José C
JC
D002478:Cells, Cultured; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D009862:Online Systems; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013302:Streptomyces / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors