Active-site assembly in glutaminyl-tRNA synthetase by tRNA-mediated induced fit.
Structure-based mutational analysis was employed to probe an unusual intramolecular interaction between partially buried glutamate residues adjacent to the active site of Escherichia coli glutaminyl-tRNA synthetase (GlnRS). The crystal structures of unliganded GlnRS and the GlnRS-tRNA(Gln) complex reveal that the Glu34 and Glu73 side chain carboxylates contact each other only in the tRNA-bound state and that the interaction is formed via mutual induced-fit transitions that occur en route to the ground-state Michaelis complex. Steady-state and transient kinetic analysis of mutant enzymes suggest that the formation of this intermolecular contact is a key event that facilitates the proper formation of the active site. Mutants at both positions destabilize the binding of the substrate glutamine at the opposite side of the active-site cleft, whereas Glu73 appears to play an additional important role by promoting the correct binding of the 3'-acceptor end of tRNA adjacent to both ATP and glutamine. The data suggest the existence of multiple structural pathways by which the binding of tRNA propagates conformational transitions leading to the proper formation of the glutamine binding site. The single-turnover kinetic analysis also establishes that the Glu34 carboxylate does not play a direct enzymatic role as a catalytic base to help deprotonate the tRNA-A76 nucleophilic 2'-hydroxyl group. The elimination of this previously proposed mechanism, together with recent chemical modification experiments in the histidyl-tRNA synthetase system, emphasizes that substrate-assisted catalysis by the phosphate of the aminoacyl adenylate may be a common means by which all tRNA synthetases facilitate the aminoacyl transfer step of the reaction.
Uter
Nathan T
NT
Department of Chemistry and Biochemistry, University of California at Santa Barbara, Santa Barbara, California 93106-9510, USA.
Perona
John J
JJ
D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D003461:Crystallography; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000201:enzymology*; D018698:Glutamic Acid / Q000737:chemistry / Q000235:genetics; D005973:Glutamine / Q000737:chemistry; D011487:Protein Conformation; D012351:RNA, Transfer, Gln / Q000737:chemistry*; D046249:Transfer RNA Aminoacylation
D012351:RNA, Transfer, Gln; D005973:Glutamine; D018698:Glutamic Acid; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase