title: Semen changes in boars after experimental infection with porcine reproductive and respiratory syndrome (PRRS) virus.
abstract: Eleven boars seronegative to porcine reproductive and respiratory syndrome virus (PRRSV) were trained for semen collection: five boars were inoculated intranasally with 6 x 10(6)TCID(50)/ml of PRRSV (Group A); four boars were inoculated intranasally with 6 x 10(4)TCID(50)/ml (Group B); and two boars were used as uninfected control (Group C). Semen samples were collected at 7-d intervals from 49 d prior to experimental inoculation with PRRSV to 70 d after inoculation, and were examined for sperm volume, sperm concentration, sperm morphology, sperm motility and for the presence of PRRSV. The infection in boars was demonstrated by the reisolation of PRRSV from the serum of all inoculated boars. Rectal temperatures and general health of the boars were clinically normal throughout the trial. Differences were observed in the quality of semen collected from boars after experimental infection with PRRSV. This infection induced a significant decrease in sperm motility and in spermatozoa with normal acrosomes. Of the semen samples tested for virus isolation in swine alveolar macrophages PRRSV was only isolated in 1 boar from Group B. The virus was detected in an additional semen sample in Group A by the production of an antibody titer in a biological assay. All attempts to detect PRRSV by RT-PCR in semen samples were unsuccessful. Nevertheless, from our study it is possible to suggest that the PRRSV can occasionally be transmitted in the semen during the initial phase of the disease.
authors.affiliation: Departamento de Patología Animal I. Universidad Complutense de Madrid, Facultad de Veterinaria 28040 Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Prieto
authors.forename: P
authors.initials: P
authors.lastname: Suárez
authors.forename: J M
authors.initials: JM
authors.lastname: Bautista
authors.forename: R
authors.initials: R
authors.lastname: Sánchez
authors.forename: S M
authors.initials: SM
authors.lastname: Rillo
authors.forename: I
authors.initials: I
authors.lastname: Simarro
authors.forename: A
authors.initials: A
authors.lastname: Solana
authors.forename: J M
authors.initials: JM
authors.lastname: Castro