HPLC determination of a novel aroylhydrazone iron chelator (o-108) in rabbit plasma and its application to a pilot pharmacokinetic study.
A high performance liquid chromatographic method for the determination of a biocompatible iron chelator, pyridoxal 2-chlorobenzoyl hydrazone (o-108), in rabbit plasma was developed and validated. The separation was achieved on a C18 column with the mobile phase composed of a mixture of 0.01 M phosphate buffer (pH 6) with the addition of EDTA (2 mM), methanol and acetonitrile (42:24:14; v/v/v). The method was validated with respect to selectivity, linearity (0.8-150 microg/mL), intra- and inter-day variability and stability. This method was successfully applied to the analysis of the samples obtained from a pilot pharmacokinetic experiment, in which the chelator was administered intravenously to rabbits.
Kovaríková
Petra
P
Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic. Petra.Kovarikova@faf.cuni.cz
Klimes
Jirí
J
Sterba
Martin
M
Popelová
Olga
O
Gersl
Vladimír
V
Ponka
Premysl
P
D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006835:Hydrazones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D007502:Iron Chelating Agents / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D015394:Molecular Structure; D010865:Pilot Projects; D011730:Pyridoxal / Q000031:analogs & derivatives / Q000097:blood / Q000493:pharmacokinetics; D011817:Rabbits; D015203:Reproducibility of Results
D006835:Hydrazones; D007502:Iron Chelating Agents; C507461:pyridoxal 2-chlorobenzoyl hydrazone; D011730:Pyridoxal