title: Effect of different extenders on glutamic oxalacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) release from frozen buffalo semen.
abstract: Sixty buffalo semen samples (motility greater than 60%) were frozen in 3 extenders, viz., Tris yolk glycerol (TY-G), Citric acid whey glycerol (CAW-G) and Egg yolk glucose sodium bicarbonate glycerol (EYGSB-G) for studying the release of GOT and GPT enzymes in the extracellular fluid during pre-freezing (after first extension) and post-freezing (15 minutes and 30 days after freezing). Release of GOT and GPT enzymes was less in TY-G than CAW-G and EYGSB-G extenders. Significant differences (P<0.01) in GOT and GPT release were observed between extenders and bulls at various stages of freezing of semen.
authors.affiliation: Department of Animal Science, Punjab Agricultural University, Ludhiana, Punjab, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Tuli
authors.forename: M
authors.initials: M
authors.lastname: Singh
authors.forename: J S
authors.initials: JS
authors.lastname: Matharoo