[Determnination of betaine in Fufang Guilu granule by HPLC].
OBJECTIVE
To describe a HPLC method for assessing betaine in Fufang Guilu granule.


METHODS
The content of betainephenaxcyl bromide in Fufang Guilu granule was determined by HPLC. The analytical column was a shim-pack CLC-ODS (6.0 mm x 150 mm) filling a 5 microm stationary phase; The mobile phase consisted of acetonitrile-water(35:65) with 0.1 mol x L(-1) NaClO4; The flow-rate was 1 mL x min (-1); The detector was set at 254 nm.


RESULTS
The calibration curve was linear over the range of 0.09-0.585 microg (r = 0.9997). The average recovery of the method was 98.4%, RSD 2.5% (n = 5).


CONCLUSIONS
The results showed that this method was reliable and accurate, and can be used for quality control of Fufang Guilu granule.
Xu
Ming-Wang
MW
Affiliated Liyuan Hospital, Mongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, China. ligenluoxi@yahoo.com.cn
Yuan
Xiu-Zhi
XZ
Liu
Yan-Weng
YW
Shen
Xu-Ju
XJ
D001622:Betaine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029745:Codonopsis / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D032305:Lycium / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D001622:Betaine