title: Characterization of the CD8+ T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice.
abstract: The BALB/c mouse model for human respiratory syncytial virus infection has contributed significantly to our understanding of the relative role for CD4+ and CD8+ T cells to immune protection and pathogenic immune responses. To enable comparison of RSV-specific T cell responses in different mouse strains and allow dissection of immune mechanisms by using transgenic and knockout mice that are mostly available on a C57BL/6 background, we characterized the specificity, level and functional capabilities of CD8+ T cells during primary and secondary responses in lung parenchyma, airways and spleens of C57BL/6 mice. During the primary response, epitopes were recognized originating from the matrix, fusion, nucleo- and attachment proteins, whereas the secondary response focused predominantly on the matrix epitope. C57BL/6 mice are less permissive for hRSV infection than BALB/c mice, yet we found CD8+ T cell responses in the lungs and bronchoalveolar lavage, comparable to the responses described for BALB/c mice.
authors.affiliation: Department of Pediatrics, The Wilhelmina Children's Hospital, University Medical Center, KE.04.133.1, Lundlaan 6, 3584 EA Utrecht, The Netherlands.
authors.forename: Michaël V
authors.initials: MV
authors.lastname: Lukens
authors.forename: Erwin A W
authors.initials: EA
authors.lastname: Claassen
authors.forename: Patricia M A
authors.initials: PM
authors.lastname: de Graaff
authors.forename: Mariska E A
authors.initials: ME
authors.lastname: van Dijk
authors.forename: Peter
authors.initials: P
authors.lastname: Hoogerhout
authors.forename: Mireille
authors.initials: M
authors.lastname: Toebes
authors.forename: Ton N
authors.initials: TN
authors.lastname: Schumacher
authors.forename: Robbert G
authors.initials: RG
authors.lastname: van der Most
authors.forename: Jan L L
authors.initials: JL
authors.lastname: Kimpen
authors.forename: Grada M
authors.initials: GM
authors.lastname: van Bleek
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology* / Q000472:pathogenicity*; D013047:Specific Pathogen-Free Organisms; D014764:Viral Proteins / Q000737:chemistry / Q000276:immunology
chemical_list: D010455:Peptides; D014764:Viral Proteins