title: Developmental competence of bovine embryos derived from oocytes collected at various stages of the estrous cycle.
abstract: The developmental competence of bovine oocytes collected from donors at various stages of the estrous cycle and fertilized in vitro was investigated by comparing the yields of embryos obtained from oocytes isolated from the ovaries of cows slaughtered on estrous cycle Days 7 and 14, 8 and 15, 9 and 16 and on Days 19, 20 and 2. The percentages of oocytes that developed into blastocysts at Day 8 after exposure to spermatozoa were: 11.9 vs 20.0; 13.2 vs 30.5; 20.8 vs 29.8; and 11.7, 4.4 and 16.9, respectively. A significantly higher proportion of oocytes developed into blastocysts following isolation on cycle Days 14 to 16 (24.3 %) than following recovery on Days 7 to 9 (13.0 %; P < 0.05), Days 19 to 20 (6.6 %; P < 0.05) or Day 2 (16.9 %; P < 0.05). Embryo development was also faster in oocytes isolated at the end of the luteal phase (Days 14 to 16). These results demonstrate that the stage of the estrous cycle may influence the developmental potential of oocytes and in vitro embryo production.
authors.affiliation: Research and Developmental Center, State Breeding Enterprise, Prague, The Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Machatková
authors.forename: E
authors.initials: E
authors.lastname: Jokesová
authors.forename: J
authors.initials: J
authors.lastname: Petelíková
authors.forename: V
authors.initials: V
authors.lastname: Dvorácek