title: Measurement of the arylsulphatase of Patella vulgata with 4-methylumbelliferone sulphate.
abstract: 1. The preparation, purification, chemical and spectral properties of potassium 4-methylumbelliferone sulphate are described. 2. The use of 4-methylumbelliferone sulphate as a substrate for the arylsulphatase of Patella vulgata is presented with specific reference to the fluorimetric assay procedure used with this substrate. 3. 4-Methylumbelliferone sulphate is compared with the previously used synthetic sulphatase substrates nitrocatechol sulphate and p-nitrophenyl sulphate with respect to K(m), V(max.) and sensitivity in the assay of arylsulphatase. 4. 4-Methylumbelliferone sulphate was strongly inhibited by phosphate. Sulphate, a less potent inhibitor, appeared to be of the competitive type with some anomalous characteristics.
authors.affiliation: Department of Psychiatry, Washington University School of Medicine, St Louis, Mo., U.S.A.
authors.forename: W R
authors.initials: WR
authors.lastname: Sherman
authors.forename: E F
authors.initials: EF
authors.lastname: Stanfield