title: Extracellular matrix proteins and basement membrane: their identification in bovine ovaries and significance for the attachment of cultured preantral follicles.
abstract: Described in the present paper is the immunolocalization of the extracellular matrix proteins (e.g., fibronectin, collagen Types I and III) in the bovine ovary, with special attention to preantral follicles. In addition, we have shown, histochemically and ultrastructurally, that mechanically isolated bovine preantral follicles are surrounded by an intact basement membrane. After 24 h of culture in serum-free medium, only 20.4% of these follicles attached to a plastic substrate. We showed that covering the plastic with extracellular matrix proteins (i.e., fibronectin, collagen Type I and matrigel) significantly increased the percentage of attached follicles to 76.0, 65.2 and 80.4%, respectively, while laminin had no effect (18.6%). When preantral follicles were embedded within three-dimensional collagen gels, no loss of follicles was observed. Restoring surface interactions between preantral follicles and the extracellular matrix in vitro, either in a two- or a three-dimensional system, might be important for maintaining follicular viability and growth in the future.
authors.affiliation: Department of Animal Endocrinology and Reproduction, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
authors.forename: S C
authors.initials: SC
authors.lastname: Hulshof
authors.forename: M
authors.initials: M
authors.lastname: Thiry
authors.forename: R
authors.initials: R
authors.lastname: Van den Hurk
authors.forename: M M
authors.initials: MM
authors.lastname: Bevers
authors.forename: B
authors.initials: B
authors.lastname: Nusgens
authors.forename: J F
authors.initials: JF
authors.lastname: Beckers