title: Expression of foreign type I ribulose-1,5-bisphosphate carboxylase/ oxygenase (EC 4.1.1.39) stimulates photosynthesis in cyanobacterium Synechococcus PCC7942 cells.
abstract: A reporter gene assay revealed that promoters derived from Synechococcus PCC7942 (S.7942) psbAI and Synechocystis PCC6803 (S.6803) psbAII were suitable for the expression of foreign ribulose-bisphosphate carboxylase (RuBisCO; EC 4.1.1.39) in S.7942 cells. Transformational vectors with a promoter and a foreign RuBisCO gene, cvrbc originated from Allochromatium vinosum, were constructed on a binary vector, pUC303, and introduced to S.7942 cells. When the cvrbc was expressed with the S.7942 psbAI promoter, the total RuBisCO activity increased 2.5- to 4-fold than that of the wild type cell. The S.6803 psbAII promoter increased the activity of the transformant 1.5-2 times of that of wild type cell. There was a significant increase in the rate of photosynthesis depending on the increase of RuBisCO activity. The maximum rate of photosynthesis of the transformant cell was 1.63 times higher than that of the wild type under the illumination of 400 micromol m(-2) s(-1), at 20 mM bicarbonate and at 30 degrees C. Although the photosynthesis of the higher plant is limited by the ability of photosystems under high irradiance and the high CO(2 )concentration, that of the S.7942 cell is limited by the RuBisCO activity, even at high CO(2) concentrations and under high irradiance.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Gakuen-cho 1-2, 599-8231 Osaka, Sakai, Japan. wadano@bioinfo.osakafu-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Iwaki
authors.forename: K
authors.initials: K
authors.lastname: Haranoh
authors.forename: N
authors.initials: N
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Kojima
authors.forename: R
authors.initials: R
authors.lastname: Satoh
authors.forename: T
authors.initials: T
authors.lastname: Nishino
authors.forename: S
authors.initials: S
authors.lastname: Wada
authors.forename: H
authors.initials: H
authors.lastname: Ihara
authors.forename: S
authors.initials: S
authors.lastname: Tsuyama
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: A
authors.initials: A
authors.lastname: Wadano
mesh_terms: D015870:Gene Expression; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics* / Q000378:metabolism*; D046940:Synechococcus / Q000235:genetics* / Q000378:metabolism*; D014169:Transformation, Bacterial
chemical_list: D012273:Ribulose-Bisphosphate Carboxylase; D010100:Oxygen