[Separation and purification of skeletal muscle satellite cells for tissue engineering applications by Percoll].
OBJECTIVE
To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique.


METHODS
The primary skeletal muscle satellite cells of New Zealand white rabbits were cultured with different adhesion time method and incontinuous density Percoll gradient centrifugation technique. The cells were observed under invert microscope and scanning electron microscope. The degree of purification was examined by celluar immunochemical stain. The growth curve was tested by thiazolyl blue assay.


RESULTS
Over 90% satellite cells were harvested by incontinuous density Percoll gradient centrifugation technique, in contrast to which, only 30%-40% cells were harvested by different adhesion time. Morphological observation accorded with satellite cells. The growth curve indicated that the cells grew in a good status.


CONCLUSIONS
The high purification satellite cells can be obtained by incontinuous density Percoll gradient centrifugation technique. It is a good method to culture seed cells for tissue engineering applications.
Hang
Yu-shi
YS
Department of Urology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. zhangyushi@126.com
Li
Han-zhong
HZ
Zhang
Rui-qiang
RQ
Wang
Peng
P
Yan
Wei-gang
WG
D000818:Animals; D021522:Bioartificial Organs; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D008297:Male; D011205:Povidone; D011817:Rabbits; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology*; D012822:Silicon Dioxide; D023822:Tissue Engineering; D001743:Urinary Bladder
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone