title: Oxidative damage of periodontal tissue in the rat periodontitis model: effects of a high-cholesterol diet.
abstract: Studies suggest an association between consumption of a high-cholesterol diet and periodontitis. We addressed the mechanism by which high dietary cholesterol could be detrimental to periodontal health in a rat model. Feeding a high-cholesterol diet augmented the effects of bacterial pathogens and their products (e.g., lipopolysaccharide and proteases) on production of pro-inflammatory cytokines in fibroblasts. High dietary cholesterol also increased mitochondrial 8-hydroxydeoxyguanosine in the periodontal tissues. These results suggest that excessive tissue oxidative damage induced by high dietary cholesterol could potentiate pro-inflammatory cytokine production by fibroblasts stimulated with bacterial pathogens.
authors.affiliation: Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8525, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Tomofuji
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Azuma
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kusano
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sanbe
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ekuni
authors.forename: Naofumi
authors.initials: N
authors.lastname: Tamaki
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000821:Animal Feed; D000818:Animals; D002784:Cholesterol / Q000008:administration & dosage* / Q000097:blood / Q000494:pharmacology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004195:Disease Models, Animal; D005347:Fibroblasts; D007375:Interleukin-1 / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010518:Periodontitis / Q000139:chemically induced / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D007375:Interleukin-1; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002784:Cholesterol; D003849:Deoxyguanosine