Propofol, an anesthetic possessing neuroprotective action against oxidative stress, promotes the process of cell death induced by H2O2 in rat thymocytes.
Propofol (2,6-diisopropylphenol) is a general anesthetic possessing a neuroprotective action against oxidative stress produced by H2O2. H2O2 induces an exposure of phosphatidylserine on outer surface of cell membranes, resulting in change in membrane phospholipid arrangement, in rat thymocytes. Since propofol is highly lipophilic, the agent is presumed to interact with membrane lipids and hence to modify the cell vulnerability to H2O2. Therefore, to test the possibility, we have examined the effect of propofol on rat thymocytes simultaneously incubated with H2O2. Although propofol (up to 30 microM) alone did not significantly affect the cell viability, the agent at 10 microM started to increase the population of dead cells in the presence of 3 mM H2O2 and the significant increase was observed at 30 microM. Propofol at clinically relevant concentrations (10-30 microM) facilitated the process of cell death induced by H2O2 in rat thymocytes. However, propofol protected rat brain neurons against the oxidative stress induced by H2O2 under same experimental condition. Therefore, the action of propofol may be dependent on the type of cells.
Chikutei
Ken-ichi
K
Department of Pharmaceutical Care and Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8512, Japan.
Oyama
Tomohiro M
TM
Ishida
Shiro
S
Okano
Yoshiro
Y
Kobayashi
Masako
M
Matsui
Hiroko
H
Horimoto
Kanna
K
Nishimura
Yumiko
Y
Ueno
Shin-ya
SY
Oyama
Yasuo
Y
D018686:Anesthetics, Intravenous / Q000494:pharmacology; D000818:Animals; D017304:Annexin A5 / Q000737:chemistry / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011419:Propidium / Q000737:chemistry / Q000378:metabolism; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D018686:Anesthetics, Intravenous; D017304:Annexin A5; D018696:Neuroprotective Agents; D011419:Propidium; D006861:Hydrogen Peroxide; D016650:Fluorescein-5-isothiocyanate; D015742:Propofol