Behavioral effects of 26RFamide and related peptides.
A novel 26-amino acid peptide possessing the Arg-Phe-NH(2) motif at its C-terminal extremity has been recently characterized and named 26RFamide (26RFa). The 26RFa precursor encompasses several potential cleavage sites and thus may generate various mature peptides including an N-terminally extended form of 26RFa (termed 43RFa), two fragments of 26RFa (26RFa(1-16) and 26RFa(20-26)), and a 9-amino acid peptide (9RFa) located in tandem in the human 26RFa precursor. In the present study, we have investigated the central effects of 26RFa and related peptides on food intake and locomotor activity in mice. We observed that i.c.v. injection of 26RFa, 43RFa, 26RFa(20-26) and 9RFa stimulated food consumption while 26RFa(1-16) and 26RFa(8-16) had no effect. A dose-dependent stimulation of locomotor activity was observed after i.c.v. administration of 26RFa, 43RFa and 26RFa(1-16), but not 26RFa(20-26), 26RFa(8-16) or 9RFa. These data indicate that the novel neuropeptides 26RFa and 43RFa act centrally to stimulate feeding and locomotor activities but the domains of the peptide involved in each of these responses are different suggesting that the two behavioral effects may be mediated through distinct receptors.
do Rego
Jean-Claude
JC
CNRS FRE 2735, Laboratoire de Neuropsychopharmacologie Expérimentale, Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (IFRMP 23), U.F.R. de Médecine et Pharmacie, Université de Rouen, 76183 Rouen, France.
Leprince
Jérôme
J
Chartrel
Nicolas
N
Vaudry
Hubert
H
Costentin
Jean
J
D000577:Amides; D000595:Amino Acid Sequence; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009043:Motor Activity / Q000187:drug effects; D009479:Neuropeptides / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D010455:Peptides / Q000235:genetics / Q000502:physiology*
D000577:Amides; D009479:Neuropeptides; D010455:Peptides; C480638:prepro-26RFa protein, human