Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap.
Biomimetic heterogeneous patterning of hepatic and endothelial cells, which start from randomly distributed cells inside the microfluidic chamber, via the chip design of enhanced field-induced dielectrophoresis (DEP) trap is demonstrated and reported in this paper. The concentric-stellate-tip electrode array design in this chip generates radial-pattern electric fields for the DEP manipulation of the live liver cells. By constructing the geometric shape and the distribution of stellate tips, the DEP electrodes enhance the desired spatial electric-field gradients to guide and snare individual cells to form the desired biomimetic pattern. With this proposed microfluidic chip design, the original randomly distributed hepatocytes inside the microfluidic chamber can be manipulated in parallel and align into the desired pearl-chain array pattern. This radial pattern mimics the lobular morphology of real liver tissue. The endothelial cells, then, are snared into the additional pearl-chain array and settle at the space in-between the previous hepatic pearl-chain array. By this cell-lab chip, we demonstrate the in vitro reconstruction of the heterogeneous lobule-mimetic radial pattern with good cell viability after cell patterning. This work reports the rapid in-parallel patterning of the dual types of live liver cells via the enhanced DEP trap inside the microfluidic chip.
Ho
Chen-Ta
CT
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 300, ROC.
Lin
Ruei-Zeng
RZ
Chang
Wen-Yu
WY
Chang
Hwan-You
HY
Liu
Cheng-Hsien
CH
D040761:Biomimetic Materials; D002448:Cell Adhesion / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D003198:Computer Simulation; D004566:Electrodes; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D008099:Liver / Q000166:cytology / Q000502:physiology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D011107:Polylysine / Q000737:chemistry
D011107:Polylysine