[Progress of the PCR amplification techniques for chromosome walking].
Various PCR-based methods are available for chromosome walking from a known sequence to an unknown region. It is promising in genome-related research for the following experiments: promoter cloning, obtaining non-conservative parts of genes in new species, identification of T-DNA or transposon insertion sites and filling in gaps or unknown chromosome regions in genome sequencing. These methods consisted of three types: inverse PCR, ligation-mediated PCR and specific primer PCR. In this review, we illustrated and compared the current techniques.
Liu
Bo
B
School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116023, China. ppic9k@msn.com
Su
Qiao
Q
Tang
Min-Qian
MQ
Yuan
Xiao-Dong
XD
An
Li-Jia
LJ
D000818:Animals; D016386:Chromosome Walking / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers