title: Transfection assays with protoplasts containing integrated reporter genes.
abstract: Transient expression assays with protoplasts that utilize stably integrated reporter genes along with transfected effector genes provide several advantages over assays in which both the reporter gene and effector gene(s) are transfected into protoplasts. A protocol for carrying out transient expression assays with Arabidopsis leaf mesophyll protoplasts containing single-copy integrated reporter genes is described.
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia, USA.
authors.forename: Shiv
authors.initials: S
authors.lastname: Tiwari
authors.forename: Shucai
authors.initials: S
authors.lastname: Wang
authors.forename: Gretchen
authors.initials: G
authors.lastname: Hagen
authors.forename: Tom J
authors.initials: TJ
authors.lastname: Guilfoyle
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins; D001681:Biological Assay; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D005821:Genetic Techniques; D005966:Glucuronidase; D018515:Plant Leaves; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000378:metabolism*; D014162:Transfection; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins; D005966:Glucuronidase