The complete nucleotide sequence and genomic organization of Citrus Leprosis associated Virus, Cytoplasmatic type (CiLV-C).
The Citrus leprosis disease (CiL) is associated to a virus (CiLV) transmitted by Brevipalpus spp. mites (Acari: Tenuipalpidae). CiL is endemic in Brazil and its recently spreading to Central America represents a threat to citrus industry in the USA. Electron microscopy images show two forms of CiLV: a rare nuclear form, characterized by rod-shaped naked particle (CiLV-N) and a common cytoplasmic form (CiLV-C) associated with bacilliform-enveloped particle and cytoplasmic viroplasm. Due to this morphological feature, CiLV-C has been treated as Rhabdovirus-like. In this paper we present the complete nucleotide sequence and genomic organization of CiLV-C. It is a bipartite virus with sequence similarity to ssRNA positive plant virus. RNA1 encodes a putative replicase polyprotein and an ORF with no known function. RNA2 encodes 4 ORFs. pl5, p24 and p61 have no significant similarity to any known proteins and p32 encodes a protein with similarity to a viral movement protein. The CiLV-C sequences are associated with typical symptoms of CiL by RT-PCR. Phylogenetic analysis suggests that CiLV-C is probably a member of a new family of plant virus evolutionarily related to Tobamovirus.
Pascon
Renata C
RC
Alellyx Applied Genomics, Techno Park (Rod. Anhanguera Km 104), Rua James Clerk Maxwell 320, 13069-380, Campinas, SP, Brazil.
Kitajima
João Paulo
JP
Breton
Michèle C
MC
Assumpção
Laura
L
Greggio
Christian
C
Zanca
Almir S
AS
Okura
Vagner Katsumi
VK
Alegria
Marcos C
MC
Camargo
Maria E
ME
Silva
Giovana G C
GG
Cardozo
Jussara C
JC
Vallim
Marcelo A
MA
Franco
Sulamita F
SF
Silva
Vitor H
VH
Jordão
Hamilton
H
Oliveira
Fernanda
F
Giachetto
Poliana F
PF
Ferrari
Fernanda
F
Aguilar-Vildoso
Carlos I
CI
Franchiscini
Fabrício J B
FJ
Silva
José M F
JM
Arruda
Paulo
P
Ferro
Jesus A
JA
Reinach
Fernando
F
da Silva
Ana Cláudia Rasera
AC
D001483:Base Sequence; D032084:Citrus sinensis / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000145:classification / Q000235:genetics*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D012367:RNA, Viral