title: Global analysis of gene function in yeast by quantitative phenotypic profiling.
abstract: We present a method for the global analysis of the function of genes in budding yeast based on hierarchical clustering of the quantitative sensitivity profiles of the 4756 strains with individual homozygous deletion of nonessential genes to a broad range of cytotoxic or cytostatic agents. This method is superior to other global methods of identifying the function of genes involved in the various DNA repair and damage checkpoint pathways as well as other interrogated functions. Analysis of the phenotypic profiles of the 51 diverse treatments places a total of 860 genes of unknown function in clusters with genes of known function. We demonstrate that this can not only identify the function of unknown genes but can also suggest the mechanism of action of the agents used. This method will be useful when used alone and in conjunction with other global approaches to identify gene function in yeast.
authors.affiliation: Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305-5152, USA. mbrown@stanford.edu
authors.forename: James A
authors.initials: JA
authors.lastname: Brown
authors.forename: Gavin
authors.initials: G
authors.lastname: Sherlock
authors.forename: Chad L
authors.initials: CL
authors.lastname: Myers
authors.forename: Nicola M
authors.initials: NM
authors.lastname: Burrows
authors.forename: Changchun
authors.initials: C
authors.lastname: Deng
authors.forename: H Irene
authors.initials: HI
authors.lastname: Wu
authors.forename: Kelly E
authors.initials: KE
authors.lastname: McCann
authors.forename: Olga G
authors.initials: OG
authors.lastname: Troyanskaya
authors.forename: J Martin
authors.initials: JM
authors.lastname: Brown
mesh_terms: D016000:Cluster Analysis; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D017353:Gene Deletion; D005800:Genes, Fungal / Q000502:physiology*; D008722:Methods; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*