title: Development and validation of a capillary electrophoresis method for the determination of sulfate in effervescent tablets.
abstract: A suitable capillary electrophoresis (CE) method was developed and validated for sulfate anion determination in effervescent tablets of Digedryl. The large excess of other ions in the matrix (i.e. excipients) constituted the main difficulty of this method's development. So an original analytical procedure for both the conditioning and rinsing of the capillary was purposed including a running electrolyte constituted by boric acid 20 mM and hexamethonium dibromide 0.75 mM at pH 8.00. Separation was carried out on a 60.2 cm (50 cm to the detector) x 0.75 microm i.d. fused-silica capillary at a potential of -29 kV and 35 degrees C. Indirect UV detection was performed at a wavelength of 254 nm using a background electrolyte containing potassium chromate. Nitrate anion was used as an internal standard for quantification. This CE method was validated in terms of selectivity, linearity, accuracy and precision.
authors.affiliation: Laboratoire de Chimie Analytique, UFR de Pharmacie, Université Victor Segalen Bordeaux 2, Bordeaux, France.
authors.forename: Helen Ho
authors.initials: HH
authors.lastname: Sung
authors.forename: Evelyne
authors.initials: E
authors.lastname: Laborde-Kummer
authors.forename: Karen
authors.initials: K
authors.lastname: Gaudin
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Dubost
mesh_terms: D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013431:Sulfates / Q000737:chemistry*; D013463:Sulfuric Acid Esters / Q000737:chemistry; D013607:Tablets / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations; D013431:Sulfates; D013463:Sulfuric Acid Esters; D013607:Tablets; D012822:Silicon Dioxide