title: [Study on induction of polyploidy in Salvia bowleyana by colchicine treatment].
abstract: OBJECTIVE
  To explore the technique of induction of polyploidy in Salvia bowleyana by colchicine treatment.
  
  
  METHODS
  The three kinds of explant of bud, leaf and calli were induced by colchicine treatment.
  
  
  RESULTS
  The induction effects were better when the calli was treated by colchicines (15 mg x L(-1)) and the leaf was pre-cultured for one week. The doubling rate was 33.33%, while the majority were wholy doubled plants, and the leaves were thicker and broader, the color was darker, the root was thicker and the stoma size was obviously bigger than the diploid plants. The number of chromosome were 8 to 64. Isoenzyme analysis showed that the enzyme activities between the polyploid and the diploid plants were quite different.
  
  
  CONCLUSIONS
  Induction of polyploidy by colchicine treatment is efficacious. The part of the doubled plants were identified as homologmous tetraploids.
authors.affiliation: Department of Biology, Tangshan Teacher's College, Tangshan 063000, China. dyz03247@sohu.com
authors.forename: Ying-Zi
authors.initials: YZ
authors.lastname: Duan
authors.forename: Shao-Ying
authors.initials: SY
authors.lastname: Ke
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.forename: Ying-Ze
authors.initials: YZ
authors.lastname: Niu
authors.forename: Chao-Zhong
authors.initials: CZ
authors.lastname: Peng
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D003078:Colchicine / Q000494:pharmacology*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D018520:Plant Shoots / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D011123:Polyploidy; D027544:Salvia / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development
chemical_list: D003078:Colchicine