title: Aspects of follicle and oocyte stage that affect in vitro maturation and development of bovine oocytes.
abstract: Success of in vitro maturation (IVM) and production of bovine embryos as related to aspects of follicle source and oocyte size were evaluated. First, it was determined that bovine oocytes continue growing in all follicular sizes studied, including >1- to 15-mm follicles. Populations of oocytes were collected from surface visible (peripheral) and cortical follicles from the same ovaries. When the number of oocytes from both peripheral and cortical follicles was combined, the yield of oocytes was approximately double that collected from 1 ovarian site alone. Oocytes from cortical follicles were smaller than those from the surface population, and the smaller cortical oocytes had a lower potential for both meiotic maturation and embryo development Only cortical oocytes with the largest diameters underwent IVM and subsequently developed to blastocysts at rates comparable to oocytes from peripheral follicles. As the diameter of the oocytes recovered from peripheral follicles increased, so did their developmental potential. When the stage of the estrous cycle was observed, it was found to have no effect on developmental potential. Finally, oocytes which extruded polar bodies at an earlier time during maturation were, on average, larger than those which extruded polar bodies later. The results serve a practical purpose in assisting selection of oocytes capable of developing into blastocysts and they give useful correlates of oocyte competencies based on knowledge of follicle source and oocyte stage.
authors.affiliation: Department of Meat and Animal Science, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: T
authors.initials: T
authors.lastname: Arlotto
authors.forename: J L
authors.initials: JL
authors.lastname: Schwartz
authors.forename: N L
authors.initials: NL
authors.lastname: First
authors.forename: M L
authors.initials: ML
authors.lastname: Leibfried-Rutledge