title: Characterization of optical parameters with a human forearm at the region from 1.15 to 1.52 microm using diffuse reflectance measurements.
abstract: Time- and space-resolved diffuse reflectance measurements were used to identify the optical parameters, the reduced scattering and absorption coefficients, of bulk living tissue in the region from 1.15 to 1.52 microm. Although in this region the detector was limited in its temporal resolution, we applied a peak-time shift analysis successfully to determine these coefficients in a human forearm, and then determined the absorption spectrum by space-resolved diffuse reflectance measurements. The absorption spectrum of a water content of 52% determined by magnetic resonance imaging experiments is in good agreement with the absorption coefficient obtained by optical measurements. Moreover, magnetic resonance imaging measurements suggest that the deviation of the absorption coefficients from the water spectrum in the strong water absorption band is caused by the heterogeneity of water distribution in tissue: the low content of water in the skin. These findings indicate that this optical method is potentially applicable to the non-invasive measurement of water in tissue, especially in a region lower than about 1.3-1.35 microm, which may be useful in monitoring oedema and tissue swelling.
authors.affiliation: Biophysics Laboratory, Research Institute for Electronic Science, Hokkaido University, Sapporo 060-0812, Japan. gnishi@imd.es.hokudai.ac.jp
authors.forename: Goro
authors.initials: G
authors.lastname: Nishimura
authors.forename: Ikuhiro
authors.initials: I
authors.lastname: Kida
authors.forename: Mamoru
authors.initials: M
authors.lastname: Tamura
mesh_terms: D004487:Edema / Q000175:diagnosis / Q000473:pathology; D005542:Forearm / Q000000981:diagnostic imaging* / Q000473:pathology; D006801:Humans; D008279:Magnetic Resonance Imaging; D055095:Optics and Photonics / Q000295:instrumentation*; D011859:Radiography; D012031:Refractometry / Q000379:methods*; D012542:Scattering, Radiation; D017695:Soft Tissue Injuries / Q000175:diagnosis / Q000473:pathology; D013057:Spectrum Analysis; D014018:Tissue Distribution; D014867:Water / Q000737:chemistry / Q000378:metabolism
chemical_list: D014867:Water