title: A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep.
abstract: Texel sheep are renowned for their exceptional meatiness. To identify the genes underlying this economically important feature, we performed a whole-genome scan in a Romanov x Texel F2 population. We mapped a quantitative trait locus with a major effect on muscle mass to chromosome 2 and subsequently fine-mapped it to a chromosome interval encompassing the myostatin (GDF8) gene. We herein demonstrate that the GDF8 allele of Texel sheep is characterized by a G to A transition in the 3' UTR that creates a target site for mir1 and mir206, microRNAs (miRNAs) that are highly expressed in skeletal muscle. This causes translational inhibition of the myostatin gene and hence contributes to the muscular hypertrophy of Texel sheep. Analysis of SNP databases for humans and mice demonstrates that mutations creating or destroying putative miRNA target sites are abundant and might be important effectors of phenotypic variation.
authors.affiliation: Unit of Animal Genomics, Department of Animal Production, Faculty of Veterinary Medicine & Centre for Biomedical Integrative Genoproteomics, University of Liège (B43), 20 Boulevard de Colonster, 4000 Liège, Belgium.
authors.forename: Alex
authors.initials: A
authors.lastname: Clop
authors.forename: Fabienne
authors.initials: F
authors.lastname: Marcq
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeda
authors.forename: Dimitri
authors.initials: D
authors.lastname: Pirottin
authors.forename: Xavier
authors.initials: X
authors.lastname: Tordoir
authors.forename: Bernard
authors.initials: B
authors.lastname: Bibé
authors.forename: Jacques
authors.initials: J
authors.lastname: Bouix
authors.forename: Florian
authors.initials: F
authors.lastname: Caiment
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Elsen
authors.forename: Francis
authors.initials: F
authors.lastname: Eychenne
authors.forename: Catherine
authors.initials: C
authors.lastname: Larzul
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Laville
authors.forename: Françoise
authors.initials: F
authors.lastname: Meish
authors.forename: Dragan
authors.initials: D
authors.lastname: Milenkovic
authors.forename: James
authors.initials: J
authors.lastname: Tobin
authors.forename: Carole
authors.initials: C
authors.lastname: Charlier
authors.forename: Michel
authors.initials: M
authors.lastname: Georges
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002874:Chromosome Mapping; D006801:Humans; D006984:Hypertrophy; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D009154:Mutation; D055435:Myostatin; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D012756:Sheep / Q000033:anatomy & histology / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics*
chemical_list: C525911:MSTN protein, human; D035683:MicroRNAs; C525912:Mstn protein, mouse; D055435:Myostatin; D016212:Transforming Growth Factor beta