title: Polymorphisms in vitamin D receptor, osteopontin, insulin-like growth factor 1 and insulin, and their associations with bone, egg and growth traits in a layer--broiler cross in chickens.
abstract: Bone strength traits in chickens are gaining importance due to economic losses and welfare concerns associated with bone fractures and other abnormalities. A chicken F2 resource population was generated from layer and broiler genetic lines, and traits relating to bone strength, egg production, egg quality and growth rate were measured in approximately 500 F2 hens. Four biological candidate genes (vitamin D receptor, VDR; insulin, INS; insulin-like growth factor 1, IGF1; and osteopontin, SPP1) were selected for investigation. Single nucleotide polymorphisms (SNPs) were identified for each candidate gene by comparing sequences between grandparent lines. Polymerase chain reaction restriction-fragment length polymorphism or SNaPshot assays were developed to genotype the F2 population and to evaluate associations between each SNP genotype and multiple phenotypes. Significant associations (P < 0.0125) were found between VDR and bone mineral content of the humerus at 35 weeks of age; between IGF1 and SPP1 and 5-week body weight; and between INS and 55-week body weight.
authors.affiliation: Department of Animal Sciences, Iowa State University, Ames, 50011, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Bennett
authors.forename: P Y
authors.initials: PY
authors.lastname: Hester
authors.forename: D E M
authors.initials: DE
authors.lastname: Spurlock
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000502:physiology; D001842:Bone and Bones / Q000502:physiology*; D002645:Chickens / Q000235:genetics / Q000254:growth & development*; D003433:Crosses, Genetic; D005787:Gene Frequency; D005838:Genotype; D007328:Insulin / Q000235:genetics / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000502:physiology; D053495:Osteopontin; D010063:Ovum / Q000502:physiology*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018167:Receptors, Calcitriol / Q000235:genetics / Q000502:physiology; D012795:Sialoglycoproteins / Q000235:genetics / Q000502:physiology
chemical_list: D030161:Avian Proteins; D007328:Insulin; D018167:Receptors, Calcitriol; D012795:Sialoglycoproteins; D053495:Osteopontin; D007334:Insulin-Like Growth Factor I