title: Leptin cDNA cloning and its mRNA expression in plateau pikas (Ochotona curzoniae) from different altitudes on Qinghai-Tibet Plateau.
abstract: Leptin, an adipocyte-derived hormone, plays an important role in body energy homeostasis. Plateau pika (Ochotona curzoniae), an endemic and keystone species living only at 3000-5000 m above sea level on Qinghai-Tibet Plateau, is a typically high hypoxia and low temperature tolerant mammal with high resting metabolic rate (RMR), non-shivering thermogenesis (NST), and high ratio of oxygen utilization to cope with harsh plateau environment. To explore the molecular mechanism of ecological acclimation in plateau pika, we first cloned pika leptin cDNA and compared its mRNA expression in different altitudes (3200 and 3900 m) using real-time RT-PCR (Taqman probe) technology. The full-length pika leptin cDNA was 3015 with 504 bp open-reading frame encoding the precursor peptide of 167 amino acids including 21 residues of signal peptide. Pika leptin was 70-72% homologous to that of other species and was of similarly structural characteristics with other species. The pika-specific genetic diversity in leptin sequence occurred at twenty sites. With the increase in altitude, there were larger fat store and high level of ob gene expression in plateau pika. Our results indicated that leptin is sensitive to cold and hypoxia plateau environment and may play one of important roles in pika's ecological adaptation to harsh plateau environment.
authors.affiliation: Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: X Q
authors.initials: XQ
authors.lastname: Zhao
authors.forename: S C
authors.initials: SC
authors.lastname: Guo
authors.forename: H G
authors.initials: HG
authors.lastname: Li
authors.forename: D L
authors.initials: DL
authors.lastname: Qi
authors.forename: D P
authors.initials: DP
authors.lastname: Wang
authors.forename: J H
authors.initials: JH
authors.lastname: Cao
mesh_terms: D000531:Altitude; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015992:Body Mass Index; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D007793:Lagomorpha / Q000235:genetics*; D020738:Leptin / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D020738:Leptin; D012333:RNA, Messenger