title: A bicistronic subgenomic mRNA encodes both the ORF2 and ORF3 proteins of hepatitis E virus.
abstract: Hepatitis E virus replicons containing the neomycin resistance gene expressed from open reading frames (ORFs) 2 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were selected by constant exposure to G418 sulfate. Northern blot analyses detected full-length replicon RNA and a single subgenomic RNA. This subgenomic RNA, which was capped, initiated at nucleotide 5122 downstream of the first two methionine codons in ORF3 and was bicistronic; two closely spaced methionine codons in different reading frames were used for the initiation of ORF3 and ORF2 translation.
authors.affiliation: Molecular Hepatitis Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-8009, USA. jgraff@niaid.nih.gov
authors.forename: Judith
authors.initials: J
authors.lastname: Graff
authors.forename: Udana
authors.initials: U
authors.lastname: Torian
authors.forename: Hanh
authors.initials: H
authors.lastname: Nguyen
authors.forename: Suzanne U
authors.initials: SU
authors.lastname: Emerson
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D002460:Cell Line; D018387:Codon, Initiator; D006023:Glycoproteins / Q000235:genetics*; D016752:Hepatitis E virus / Q000235:genetics*; D006801:Humans; D014176:Protein Biosynthesis; D012315:RNA Caps; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D012093:Replicon; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D018387:Codon, Initiator; D006023:Glycoproteins; C400973:ORF3 protein, Hepatitis E virus; D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins