title: Effect of different equilibration times and extenders on deep freezing of buffalo semen.
abstract: Thirty semen collections from 3 Murrah buffalo bulls were frozen in Tris yolk glycerol (TY-G) and Citric acid whey glycerol (CAW-G) extenders using 2, 4 and 6 hours equilibration times and 7 percent glycerol level. Sperm motility after freezing was studied at an interval of 15 minutes 7 days and 30 days storage in liquid nitrogen. Sperm survivability was found to be better at all the stages of deep-freezing using 4 hours equilibration time. Significant differences (P<0.01) were observed between extenders and equilibration times.
authors.affiliation: Department of Animal Science, Punjab Agricultural University, Ludhiana, Punjab, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Tuli
authors.forename: M
authors.initials: M
authors.lastname: Singh
authors.forename: J S
authors.initials: JS
authors.lastname: Matharoo