[Immunoglobulin expression in colon cancer cell line HT-29 and its biological activities].
OBJECTIVE
To determine the expression of immunoglobulins in HT-29 cells (an established colon cancer cell line, and explore their effect on the biological activities of the cancer cells.)


METHODS
The transcripts of variable regions of immunoglobulin heavy chains in HT-29 cells were detected by RT-PCR. Antisense CDR3 (specific to HT-29)-pIRES 1 neo vector was constructed, then transfected into HT-29 cells by electroporation. Programmed cell death and growth inhibition of HT-29 cells were detected by FCM and MTT, respectively.


RESULTS
The transcripts of Ig heavy chain (V(H) CDR3 region) were expressed in HT-29 cells. Moreover, they showed a monoclonal characteristic after being sequenced. After transfection of the antisense vector of CDR3 (specific to HT-29)-pIRES 1 neo, expression level of Ig in HT-29 cells was significantly decreased, and growth inhibition (P < 0.05) and apoptosis (P < 0.01) were induced.


CONCLUSIONS
These results suggest that tumor derived Ig could promote the survival and growth of tumor cells.
Deng
Yu-qing
YQ
Peking University Center for Human Disease Genomics, Beijing 100083, China.
Zheng
Jie
J
Li
Guo-hui
GH
Zhu
Xiao-hui
XH
Zhang
Pei
P
Huang
Jing
J
Zhang
Ying-mei
YM
Li
Zhi-xin
ZX
Qiu
Xiao-yan
XY
D017209:Apoptosis; D049109:Cell Proliferation; D022801:Complementarity Determining Regions / Q000096:biosynthesis* / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics; D018274:Electroporation; D005822:Genetic Vectors; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis* / Q000235:genetics; D007075:Immunoglobulin M / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis* / Q000235:genetics; D007136:Immunoglobulins / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection
D022801:Complementarity Determining Regions; D016373:DNA, Antisense; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D012333:RNA, Messenger; D011994:Recombinant Proteins