Intergeneric relationship between the Aspergillus ochraceous virus F and the Penicillium stoloniferum virus S.
It was reported that the "slow" component (PsV-S) of Penicillium stoloniferum virus complex also occurred in a second genus, Aspergillus ochraceous. The responsible virus for this intergeneric occurrence was considered to be the "fast" component (AoV-F) of A. ochraceous virus complex. In this investigation, AoV dsRNA 1, that was previously shown to cross-hybridize with PsV-S dsRNA, has been cloned. It was 1754 bp in length and contained one open reading frame of 539 amino acids (p63), had the same genome organization as PsV-S dsRNA S1 and also had the conserved sequence motif of the PsV-S dsRNAs (5'-GCGCAAAA-3') at the 5' terminus. A BLAST search indicated that p63 was a putative dsRNA-dependent RNA polymerase (RdRp), had 81% of sequence homology to members of the genus Partitivirus, and grouped together with PsV-S in phylogenetic analysis. But immunoblot analysis showed that the capsid protein (P3) of AoV-F virus component did not reacted against PsV-S antiserum. These evidences suggest that the cross serological relationship between AoV-F and PsV-S previously observed may have been due to the RdRps of the respective viruses rather than between their respective capsid proteins as was assumed in 1985.
Kim
Jong Wook
JW
Department of Biochemistry, College of Medicine, Inha University, Incheon 402-751, South Korea. jwkim@inha.ac.kr
Choi
Eun Young
EY
Kim
Young Tae
YT
D001235:Aspergillus ochraceus / Q000821:virology*; D004587:Electrophoresis, Agar Gel; D010407:Penicillium / Q000821:virology*; D010802:Phylogeny; D012328:RNA Viruses / Q000201:enzymology / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D017385:Sequence Homology; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase