title: 24-Hour cooled storage of equine embryos.
abstract: Equine embryos were collected by transcervical uterine flush 7 d after ovulation. The flush solution was Dulbecco's phosphate buffered saline (PBS) with 1% newborn calf serum and penicillin-streptomycin. Each embryo was washed in modified Dulbecco's PBS with 1% newborn calf serum and 0.4% bovine serum albumin, and placed in 4-ml polystyrene test tube containing this same medium. Embryos were packaged in a commercial semen transport container which cooled (-0.3 degrees C/min) and maintained the embryo at 4 to 6 degrees C. After 24 h, 16 embryos were transcervically transferred into recipient mares. Of the 16 embryos, six were detected as vesicles by ultrasonography at 14 d of pregnancy, of which three were carried to term and resulted in live, normal foals. Sixteen control embryos were directly transferred without prior storage and resulted in five foals.
authors.affiliation: Hofmann Center for Reproductive Studies, University of Pennsylvania - New Bolton Center, Kennett Square, PA 19348, USA.
authors.forename: P L
authors.initials: PL
authors.lastname: Sertich
authors.forename: L B
authors.initials: LB
authors.lastname: Love
authors.forename: M R
authors.initials: MR
authors.lastname: Hodgson
authors.forename: R M
authors.initials: RM
authors.lastname: Kenney