In vivo culture of embryos in the immature mouse oviduct.
In-vitro culture of mammalian preimplantation embryos is associated with subsequent decreased viability. This phenomenon is more pronounced with the domestic species embryos as culture conditions are at present unable to sustain cleavage of early preimplantation embryos for more than one or two cell divisions. In this study, the immature mouse oviduct is shown to be capable of supporting cleavage and morphological development of rabbit and porcine embryos. The immature mouse oviduct was shown to be comparable to in vitro culture as 76% and 60% of the transferred zygotes developed to the morula stage after 2 and 3 d respectively. The porcine zygotes, however, failed to develop beyond the 4-cell stage in either the immature mouse oviduct or in vitro. Porcine morula showed better tolerance of the oviduct environment and when recovered after 2 d contained an average of 64 cells, which was significantly more than in in vitro cultured morulae (40 cells). Early porcine blastocysts transferred to the mouse oviduct had over a two-fold increase in cell division (104 cells) over comparable blastocysts grown in vitro (57 cells). The immature mouse oviduct is, therefore, a potential surrogate environment for short-term storage of embryos of other species.
Ebert
K M
KM
Department of Anatomy and Cellular Biology School of Veterinary Medicine, Tufts University, 200 Westboro Road, North Grafton, MA 01536 USA; Department of Anatomy and Cellular Biology, School of Medicine, Tufts University, 200 Westboro Road, North Grafton, MA 01536 USA; Department of Anatomy and Cellular Biology, School of Dental Medicine, Tufts University, 200 Westboro Road, North Grafton, MA 01536 USA.
Papaioannou
V E
VE