Extracellular potassium deprivation reversibly dephosphorylates cofilin.
Cofilin is essential for actin filament remodeling in various cell types. We previously reported that ouabain leads to dephosphorylation of cofilin, resulting in cytoskeletal reorganization and cell volume change. We report here that the extracellular [K(+)](e) deprivation causes dephosphorylation of cofilin with disruption of cytoskeletal structures and cell shrinkage. These changes can be reversed by time-dependent restoration of [K(+)](e). Thus, these results suggest that the acute shift of [K(+)](e) triggers a reversible change of cofilin phosphorylation which may be an essential event in the regulation of cytoskeletal architecture and cell volume.
Jung
Jaehoon
J
College of Pharmacy, Center for Cell Signaling Research and Division of Molecular Life Sciences, Ewha Womans University, Seoul, Republic of Korea.
Park
Heejin
H
Kim
Moonhee
M
Kim
Min-Jeong
MJ
Choi
Eung Chil
EC
Lee
Kyunglim
K
D051339:Actin Depolymerizing Factors / Q000378:metabolism*; D000818:Animals; D048429:Cell Size / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D051379:Mice; D041681:NIH 3T3 Cells; D010766:Phosphorylation / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology*; D013997:Time Factors
D051339:Actin Depolymerizing Factors; D011188:Potassium