Fast BIA-amperometric determination of isoniazid in tablets.
This paper proposes a new, fast and precise method to analyze isoniazid based on the electrochemical oxidation of the analyte at a glassy carbon electrode in 0.1M NaOH. The quantification was performed utilizing amperometry associated with batch injection analysis (BIA) technique. Fast sequential analysis (60 determinations h(-1)) in an unusually wide linear dynamic range (from 2.5 x 10(-8) to 1.0 x 10(-3)M), with high sensitivity and low limits of detection (4.1 x 10(-9)M) and quantification (1.4 x 10(-8)M), was achieved. Such characteristics allied to a good repeatability of the current responses (relative standard deviation of 0.79% for 30 measurements), were explored for the specific determination of isoniazid in isoniazid-rifampin tablet.
Quintino
Maria S M
MS
Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748-05508-900 São Paulo, SP, Brazil.
Angnes
Lúcio
L
D004563:Electrochemistry; D017022:Flow Injection Analysis / Q000379:methods*; D007538:Isoniazid / Q000032:analysis*; D015203:Reproducibility of Results; D013607:Tablets
D013607:Tablets; D007538:Isoniazid