Evaluation of methanol-water and acetonitrile-water binary mixtures as eluents for temperature-dependent inclusion chromatography.
In the present study the solubility of beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin at sub-zero and elevated temperatures (-10 and +30 degrees C) for a given composition of methanol/water and acetonitrile/water binary mixtures (Xs = 0.16) was studied. Moreover, the freezing temperature profiles of acetonitrile-based chromatographic mobile phases were measured, and the obtained results were compared with data available in the literature. Furthermore, the effect of the macrocycles concentration on the liquid-phase freezing points was determined. The low solubility of native beta-cyclodextrin in a methanol/water mixture at sub-zero temperature as well as the non-linear behavior of acetonitrile/water mixtures that were observed concerning the freezing point profile are discussed from a practical point of view.
Zarzycki
Paweł K
PK
Laboratory of Toxicology, Department of Environmental Biology, Technical University of Koszalin, Poland. pkzarz@wp.pl
Włodarczyk
Elzbieta
E
Lou
Da-Wei
DW
Jinno
Kiyokatsu
K
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000097:Acetonitriles / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D000432:Methanol / Q000737:chemistry*; D044367:Phase Transition; D015203:Reproducibility of Results; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D044366:Transition Temperature; D014867:Water / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry
D000097:Acetonitriles; D012997:Solvents; D047392:beta-Cyclodextrins; D014867:Water; D000073738:2-Hydroxypropyl-beta-cyclodextrin; C031215:betadex; D000432:Methanol; C032159:acetonitrile