Novel polyallylamine-dextran sulfate-DNA nanoplexes: highly efficient non-viral vector for gene delivery.
Polyallylamine (PAA), a less investigated polycationic polymer along with polyanionic dextran sulfate (DS) has been complexed with DNA into self assembling PAA-DS-DNA complexes with zinc as stabilizing agent. The complexes prepared were characterized by dynamic light scattering and atomic force microscopy and found to have average hydrodynamic diameter of 150nm with polydispersity below 0.2, i.e. nanoplexes with narrow size distribution. As expected, the zeta potential values increased with the increase in the PAA in the nanoplexes. Further, PAA-DS-DNA nanoplexes were investigated for in vitro transfecting efficacy on HEK 293 cells. The nanoplexes were found to have better gene transfer efficacy compared to the DNA-PAA complex and commercially available transfecting agent lipofectin. The cytotoxicity was also decreased considerably as revealed by MTT colorimetric assay. The nanoplexes prepared with PAA to DS ratio of 2 were found to be most efficient transfecting agent.
Nimesh
Surendra
S
Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110007, India.
Kumar
Rupesh
R
Chandra
Ramesh
R
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016264:Dextran Sulfate / Q000737:chemistry* / Q000633:toxicity; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008027:Light; D008055:Lipids; D018625:Microscopy, Atomic Force; D053758:Nanoparticles; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry* / Q000633:toxicity; D012542:Scattering, Radiation; D014162:Transfection; D015032:Zinc / Q000737:chemistry
D008055:Lipids; C086724:Lipofectamine; D011073:Polyamines; D049452:Green Fluorescent Proteins; C063994:polyallylamine; D004247:DNA; D016264:Dextran Sulfate; D015032:Zinc