title: Frequent rearrangements and amplification of the CDX2 homeobox gene in human sporadic colorectal cancers with chromosomal instability.
abstract: The expression of the CDX2 gene, a crucial regulator of gut homeostasis, is altered in human colorectal cancers in parallel with de-differentiation. Here, we have investigated the chromosomal status of CDX2 in human sporadic colorectal cancers with the phenotype of chromosomal instability. Allelic imbalance determination showed frequent rearrangements at the CDX2 locus. The rearrangements correlated with CDX2 gene amplification, as assessed by quantitative PCR analysis. However, they were not predictive of the Cdx2 protein pattern. These data suggest that mechanisms other than structural alterations at the CDX2 locus account for the change of expression in colorectal cancers.
authors.affiliation: Inserm U682, University Louis Pasteur, 67200 Strasbourg, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Subtil
authors.forename: Eric
authors.initials: E
authors.lastname: Guérin
authors.forename: Anne
authors.initials: A
authors.lastname: Schneider
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Chenard
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Martin
authors.forename: Claire
authors.initials: C
authors.lastname: Domon-Dell
authors.forename: Isabelle
authors.initials: I
authors.lastname: Duluc
authors.forename: Thomas
authors.initials: T
authors.lastname: Brabletz
authors.forename: Michèle
authors.initials: M
authors.lastname: Kedinger
authors.forename: Bernard
authors.initials: B
authors.lastname: Duclos
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Gaub
authors.forename: Jean-Noël
authors.initials: JN
authors.lastname: Freund
mesh_terms: D001483:Base Sequence; D000071616:CDX2 Transcription Factor; D043171:Chromosomal Instability; D015179:Colorectal Neoplasms / Q000235:genetics*; D017931:DNA Primers; D005784:Gene Amplification; D015321:Gene Rearrangement; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans
chemical_list: D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D017931:DNA Primers; D018398:Homeodomain Proteins