title: Three-dimensional imaging of monogenoidean sclerites by laser scanning confocal fluorescence microscopy.
abstract: A nondestructive protocol for preparing specimens of Monogenoidea for both alpha-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy.
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Galli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Strona
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Villa
authors.forename: Francesca
authors.initials: F
authors.lastname: Benzoni
authors.forename: Stefani
authors.initials: S
authors.lastname: Fabrizio
authors.forename: Silvia Maria
authors.initials: SM
authors.lastname: Doglia
authors.forename: Delane C
authors.initials: DC
authors.lastname: Kritsky
mesh_terms: D000818:Animals; D002347:Carps / Q000469:parasitology*; D005393:Fish Diseases / Q000469:parasitology*; D018613:Microscopy, Confocal / Q000662:veterinary*; D010473:Perciformes / Q000469:parasitology*; D010986:Platyhelminths / Q000033:anatomy & histology*; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*