title: Real-time in vivo virtual histology of colorectal lesions when using the endocytoscopy system.
abstract: BACKGROUND
  The histological findings of GI lesions are based on light-microscopic examination of H&E-stained thin-slice specimens. Recently, a concept of optical biopsy has been advocated. A study of the observation of colorectal lesions using endocytoscopy to obtain real-time histological images in vivo during endoscopy was performed.
  
  
  METHODS
  Prospective study.
  
  
  OBJECTIVE
  To evaluate the usefulness of optical biopsy of colorectal lesions with the endocytoscopy (E-C) system.
  
  
  METHODS
  The subjects were 113 consecutive patients who underwent a complete colonic examination, from April 2003 to March 2004, performed by a single colonoscopist.
  
  
  METHODS
  Digestive Disease Center of Showa University Northern Yokohama Hospital.
  
  
  RESULTS
  With E-C, it was possible to observe lesions at the cellular level and evaluate cellular atypia in addition to structural atypia in vivo. The correlation was statistically significant between the endocytoscopic diagnosis and the histological diagnosis.
  
  
  CONCLUSIONS
  The endocytoscope had to be touched to the target colonic glands.
  
  
  CONCLUSIONS
  It was possible to distinguish neoplastic from non-neoplastic lesions, and also possible to distinguish invasive cancer from adenoma. "Ultra-high" magnifying endoscopy, the E-C system, provides real-time histological images in vivo, which correspond well with those of H&E-stained microscopic images.
authors.affiliation: Digestive Disease Center, Department of Pathology, Showa University Northern Yokohama Hospital, Chigasaki-chuo 35-1, Tsuzuki-ku, Yokohama 224-8503, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Sasajima
authors.forename: Shin-ei
authors.initials: SE
authors.lastname: Kudo
authors.forename: Haruhiro
authors.initials: H
authors.lastname: Inoue
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kashida
authors.forename: Eiji
authors.initials: E
authors.lastname: Hidaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawachi
authors.forename: Masanori
authors.initials: M
authors.lastname: Sakashita
authors.forename: Junichi
authors.initials: J
authors.lastname: Tanaka
authors.forename: Akira
authors.initials: A
authors.lastname: Shiokawa
mesh_terms: D000236:Adenoma / Q000000981:diagnostic imaging / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology*; D016099:Endoscopy, Gastrointestinal / Q000379:methods*; D005260:Female; D006801:Humans; D008875:Middle Aged; D011446:Prospective Studies; D014463:Ultrasonography