[Analysis of promoter sequence and its activity of homologues gene rad25 of eukaryotes from halophilic archaea].
The transcriptional product in Halobacterium halobium R1 similar to eukaryotic gene rad25 was analyzed by RT-PCR. Using bgaH as the reporter genes, the promoter function of eukaryotic rad25-like DNA fragment in halophilic archaea was investigated by promoter probe analysis. The important functional regions, which could influence the promoter activity of rad25-like gene, were identified by deletion analysis of the promoter sequence in Haloferax volcanii. It is found that the DNA fragment of promoter similar to eukaryotic gene rad25 contains the typical characteristic sequence of archaeal promoter. These results indicate that rad25-like gene in Halobacterium halobium R1 is active and may play a role on the NER pathway as the eukaryotic pattern.
Zhu
Jian-Yu
JY
College of Life Sciences, Wuhan University, Wuhan 430072, China. csuzhu@hotmail.com
Hu
Yue-Hua
YH
Liu
Jing
J
Huang
Yu-Ping
YP
Shen
Ping
P
D019843:Archaeal Proteins / Q000235:genetics*; D001483:Base Sequence; D004265:DNA Helicases / Q000235:genetics*; D005057:Eukaryotic Cells / Q000201:enzymology*; D019617:Halobacterium salinarum / Q000201:enzymology / Q000235:genetics*; D019622:Haloferax volcanii / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
D019843:Archaeal Proteins; D004265:DNA Helicases