Study on biological characters of SGC7901 gastric cancer cell-dendritic cell fusion vaccines.
OBJECTIVE
To detect the biological characters of the SGC7901 gastric cancer cell-dendritic cell fusion vaccines.


METHODS
The suspending living SGC7901 gastric cancer cells and dendritic cells were induced to be fusioned by polyethylene glycol. Pure fusion cells were obtained by selective culture with the HAT/HT culture systems. The fusion cells were counted at different time points of culture and their growth curves were drawn to reflect their proliferative activities. The fusion cells were also cultured in culture medium to investigate whether they could grow into cell clones. MTT method was used to test the stimulating abilities of the fusion cells on T lymphocytes' proliferations. Moreover, the fusion cells were planted into nude mice to observe whether they could grow into new planted tumors in this kind of immunodeficiency animals.


RESULTS
The fusion cells had weaker proliferative activity and clone abilities than their parental cells. When they were cultured, the counts of cells did not increase remarkably, nor could they grow into cell clones in culture medium. The fusion cells could not grow into new planted tumors after planted into nude mice. The stimulating abilities of the fusion cells on T lymphocytes' proliferations were remarkably increased than their parental dendritic cells.


CONCLUSIONS
The SGC7901 gastric cancer cell-dendritic cell fusion vaccines have much weaker proliferative abilities than their parental cells, but they keep strong abilities to irritate the T lymphocytes and have no abilities to grow into new planted tumors in immunodeficiency animals. These are the biological basis for their anti-tumor biotherapies.
Zhang
Kun
K
Department of Hepatobiliary Surgery, Fuzhou General Hospital, Fujian Province, China. zhangkun73@yahoo.com.cn
Gao
Peng-Fen
PF
Yu
Pei-Wu
PW
Rao
Yun
Y
Zhou
Li-Xin
LX
D000818:Animals; D019496:Cancer Vaccines / Q000032:analysis* / Q000627:therapeutic use; D002450:Cell Communication / Q000502:physiology; D002459:Cell Fusion / Q000379:methods*; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000473:pathology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D013274:Stomach Neoplasms / Q000473:pathology*; D013601:T-Lymphocytes / Q000502:physiology; D014407:Tumor Cells, Cultured
D019496:Cancer Vaccines