Capillary electrophoresis of affinity complexes between subviral 80S particles of human rhinovirus and monoclonal antibody 2G2.
Human rhinoviruses (HRVs), the main etiologic agents of the common cold, transform into subviral B- or 80S particles (they sediment at 80S upon sucrose density gradient centrifugation) during infection and, in vitro, upon exposure to a temperature between 50 and 56 degrees C. With respect to the native virion they lack the genomic RNA and the viral capsid protein VP4. 80S particles are unstable and easily disintegrate into their components, VP1, VP2, and VP3 in buffers containing SDS. However, this detergent was found to be a necessary constituent of the BGE for the analysis of these viruses and their complexes with receptors and antibodies by CE. We here demonstrate that dodecylpoly(ethyleneglycol ether) (D-PEG) a nonionic detergent, is suitable for analysis of subviral particles as it preserves their integrity, in contrast to SDS. Electrophoresis of the 80S particles in borate buffer (pH 8.3, 100 mM) containing 10 mM D-PEG resulted in a well-defined electrophoretic peak. The identity of the peak was confirmed, among other means, by complexation with mAb 2G2, which recognizes a structural epitope exclusively present on subviral particles but not on native virus. Upon incubation of the 80S particles with mAb 2G2 the peak disappeared, but a new peak, attributed to the antibody complex emerged. The separation system allowed following the time course of the transformation of intact HRV serotype 2 into 80S particles upon incubation at temperatures between 40 and 65 degrees C. We also demonstrate that subviral particles derived from HRV2 labeled with the fluorescence dyes FITC or Cy3.5 were stable in the separation system containing D-PEG. Dye-modified particles were still recognized by mAb 2G2, suggesting that the exposed lysines that are derivatized by the reagent do not form part of the epitope of the antibody.
Kremser
Leopold
L
Institute of Analytical Chemistry, University of Vienna, Austria. leopold.kremser@univie.ac.at
Petsch
Martina
M
Blaas
Dieter
D
Kenndler
Ernst
E
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002021:Buffers; D002232:Carbocyanines / Q000737:chemistry; D002478:Cells, Cultured; D003902:Detergents / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols / Q000737:chemistry*; D012229:Rhinovirus / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D014771:Virion / Q000737:chemistry* / Q000276:immunology
D000911:Antibodies, Monoclonal; D002021:Buffers; D002232:Carbocyanines; D003902:Detergents; C089187:cyanine dye 3; C017272:dodecyloctaethyleneglycol monoether; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate