Elevated mutation rates in the germline of Polkappa mutant male mice.
Mutation rates at two expanded simple tandem repeat (ESTR) loci were studied in the germline of DNA polymerase kappa (Polkappa(-/-)) deficient mice. The spontaneous mutation rate in homozygous Polkappa(-/-) males was significantly higher than in isogenic wild-type mice (Polkappa(+/+)), but the ESTR mutation spectrum in Polkappa(-/-) animals did not differ from that in Polkappa(+/+) males. We suggest that compromised translesion synthesis in Polkappa(-/-) mice may result in replication fork pausing which, in turn, may affect ESTR mutation rate.
Burr
Karen L-A
KL
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
Velasco-Miguel
Susana
S
Duvvuri
Venkata S
VS
McDaniel
Lisa D
LD
Friedberg
Errol C
EC
Dubrova
Yuri E
YE
D000818:Animals; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000172:deficiency / Q000235:genetics*; D018095:Germ-Line Mutation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D018598:Minisatellite Repeats; D000099270:Y-Family DNA Polymerases
D004259:DNA-Directed DNA Polymerase; C498613:Polk protein, mouse; D000099270:Y-Family DNA Polymerases