A study of retinal penetration of intravitreal tenecteplase in pigs.
OBJECTIVE
To determine the degree of retinal penetration of intravitreal tenecteplase in a porcine model.


METHODS
Fluorescence-tagged tenecteplase (50 microg in 0.1 mL physiologic saline) was injected into the vitreous of the right and left eyes at 24 and 6 hours before death, respectively, in six nonvitrectomized pigs. Retinal penetration was assessed on frozen sections by epifluorescence microscopy and statistical analysis was performed. Frozen sections of two eyes without injection (control) were also assessed.


RESULTS
Labeling of fluorescence-tagged tenecteplase was seen in all the layers of the retina at both time points with more intense signal at 24 hours after injection (P<0.05).


CONCLUSIONS
Fluorescence-tagged tenecteplase can penetrate all the layers of the retina of porcine eyes after intravitreal injection. Intravitreal tenecteplase may be useful in the in the management of subretinal hemorrhage.
Kwan
Anthony S L
AS
Lions Eye Institute, Centre for Ophthalmology and Visual Science, the University of Western Australia, Nedlands, Australia.
Vijayasekaran
Sarojini
S
McAllister
Ian L
IL
Yu
Paula K
PK
Yu
Dao-Yi
DY
D000818:Animals; D001693:Biological Transport, Active; D001813:Blood-Retinal Barrier / Q000502:physiology; D005343:Fibrinolytic Agents / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes; D007267:Injections; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D012160:Retina / Q000378:metabolism*; D012166:Retinal Hemorrhage / Q000188:drug therapy; D013552:Swine; D000077785:Tenecteplase; D010959:Tissue Plasminogen Activator / Q000493:pharmacokinetics*; D014822:Vitreous Body
D005343:Fibrinolytic Agents; D005456:Fluorescent Dyes; D010959:Tissue Plasminogen Activator; D000077785:Tenecteplase