Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain.
In the adult mouse forebrain, large numbers of neuronal precursors, destined to become GABA- and dopamine-producing interneurons of the olfactory bulb (OB), are generated in the subventricular zone (SVZ). Although this neurogenic system represents a potential reservoir of stem and progenitor cells for brain repair approaches, information about the survival and differentiation of SVZ-derived cells in ectopic brain regions is still fragmentary. We show here that ectopic grafting of SVZ tissue gave rise to two morphologically distinguishable cell types displaying oligodendrocytic or astrocytic characteristics. Since SVZ tissue contains neuronal and glial progenitors, we used magnetic cell sorting to deplete A2B5+ glial progenitors from the dissociated SVZ and to positively select cells that express PSA-NCAM. This procedure allowed the purification of neuronal precursors expressing TUJ1, DCX and GAD65/67. Transplantation of these cells led again to the generation of the same two glial cell types, showing that committed interneuron precursors undergo glial differentiation outside their normal environment.
Seidenfaden
Ralph
R
Institut de Biologie du Développement de Marseille, CNRS, Université de la Méditeranée, Campus de Luminy-case 907, 13288 Marseille cedex 9, France. seidenfa@ibdm.univ-mrs.fr
Desoeuvre
Angélique
A
Bosio
Andreas
A
Virard
Isabelle
I
Cremer
Harold
H
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D016380:Brain Tissue Transplantation / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D002469:Cell Separation; D002470:Cell Survival / Q000502:physiology; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005968:Glutamate Decarboxylase / Q000378:metabolism; D007395:Interneurons / Q000166:cytology / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D020547:Lateral Ventricles / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology; D012794:Sialic Acids / Q000378:metabolism; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D014404:Tubulin / Q000378:metabolism
D015415:Biomarkers; C000714847:Dcx protein, mouse; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D007527:Isoenzymes; D008869:Microtubule-Associated Proteins; D039842:Neural Cell Adhesion Molecule L1; D009479:Neuropeptides; D012794:Sialic Acids; D014404:Tubulin; C462941:beta3 tubulin, mouse; C103469:polysialyl neural cell adhesion molecule; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2