The effect of sucrose and trehalose on viability of one- and two-cell rabbit embryos.
The effect of sucrose and trehalose on the viability of one- and two-cell rabbit embryos was investigated. A significant decrease in the viability of one- and two-cell embryos exposed for 30 min. at 20 degrees C was observed. At 38 degrees C none of the two-cell embryos in a sucrose solution survived after 30 min exposure, while approximately 50% of the embryos survived in a trehalose solution. The cleveage rate in culture of two-cell embryos exposed both to 2.0 M or 1.45 M trehalose was significantly lower in comparison with the control group. However the survival rate after transfer of two-cell embryos exposed to 1.45 M trehalose solution at 20 degrees C remained the same as that of the control group.
Smorag
Z
Z
Institute of Zootechnics Department of Animal Reproduction and AI 32-083 Balice/Krakow, Poland.
Heyman
Y
Y
Garnier
V
V
Gajda
B
B