The uptake of [Ca]calcium ions by matrix vesicles isolated from calcifying cartilage (Short Communication).
Extracellular membranous matrix vesicles, which contain various phosphatases and appear to initiate hydroxyapatite formation in growth cartilage, were isolated and incubated with (45)Ca(2+) and shown to form mineral in the presence of ATP. There is enhanced calcification in the presence of serum and under alkaline conditions.
Ali
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SY
Institute of Orthopaedics (University of London), Royal National Orthopaedic Hospital, Stanmore, Middx. HA7 4LP, U.K.
Evans
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