Biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa is associated with an unfavorable evolution after surgery for chronic sinusitis and nasal polyposis.
OBJECTIVE
To determine whether biofilm-forming capacity of bacteria demonstrated in chronic rhinosinusitis (CRS) has an impact on persistence of the disease following endoscopic sinus surgery (ESS).


METHODS
Thirty-one bacterial strains recovered from 19 patients with CRS at least 1 year post-ESS. Evolution of disease was assessed by questionnaire and endoscopy as favorable or unfavorable. The bacteria were cultured on a 96-well culture plaque and a semi-quantitative method using crystal violet to quantify biofilm production was used.


RESULTS
Twenty-two of 31 samples produced a biofilm thicker or equal to the positive control. Biofilm production was noted in 6/10 Pseudomonas aeruginosa isolates, 8/10 Staphylococcus aureus, and 8/11 coagulase-negative staphylococci. Biofilm formation was associated with a poor evolution for Pseudomonas aeruginosa and Staphylococcus aureus, but not coagulase-negative staphylococcus.


CONCLUSIONS
There is a correlation between in vitro biofilm-producing capacity by Pseudomonas aeruginosa and Staphylococcus aureus and unfavorable evolution after ESS, suggesting a role for biofilm production in chronic sinusitis.


METHODS
B-2b.
Bendouah
Zohra
Z
Department of Otolaryngology-Head and Neck Surgery, Université de Montréal, Canada.
Barbeau
Jean
J
Hamad
Walid Abou
WA
Desrosiers
Martin
M
D000328:Adult; D000368:Aged; D018441:Biofilms; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009298:Nasal Polyps / Q000382:microbiology* / Q000601:surgery; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D012852:Sinusitis / Q000382:microbiology* / Q000601:surgery; D013211:Staphylococcus aureus / Q000302:isolation & purification*