[Effect of three different curcumin pigmens on the prdiferation of vascular smooth muscle cells by ox-LDL and the expression of LDL-R].
OBJECTIVE
To investigate the anti-proliferation of three different structural curcumin pigmen monomers on the bovine VSMC stimulated by ox-LDL and the effect on the expression of LDL-R.


METHODS
MTT and FCM were used to observe the anti-proliferation of three different on the bovine VSMC stimulated by ox-LDL and the effect on the expression of LDL-R.


RESULTS
ox-LDL(2.5,5, 10 mg x L(-1)) had obvious proliferative effect on VSMC, P < 0.05, curcumin (16.5,33,66 micromol x L(-1)), demethoxycurcumin (33,66 micromol x L(-1)) and bisdemethoxycurcumin (66 micromol x L(-1)) had obvious inhibition effect on the proliferation of VSMC stimulated by 10 mg x L(-1) ox-LDL (P < 0.05, curcumin > demethoxycurcumin > bisdemethoxycurcumin) . Three curcumin (16.5,33,66 micromol x L(-1)) could obviously activate the expression of LDLR of VSMC.


CONCLUSIONS
The three different structural curcumin pigmen monomers can not only inhibit the proliferation of bovine VSMC obviously stimulated by ox-LDL, but also promote the expression of LDL-R on bovine VSMC, that may be the mechanism of delaying the development of arteriosclerosis.
Liu
Yan
Y
Zhejiang Traditional Medical College, Hangzhou 310009, China.
Hong
Xing-Qiu
XQ
D000818:Animals; D001011:Aorta / Q000166:cytology; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology*; D036381:Diarylheptanoids; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011973:Receptors, LDL / Q000378:metabolism*; D013329:Structure-Activity Relationship
D036381:Diarylheptanoids; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; C099252:oxidized low density lipoprotein; C034786:bisdemethoxycurcumin; D003474:Curcumin; C050229:demethoxycurcumin