Three-dimensional imaging of monogenoidean sclerites by laser scanning confocal fluorescence microscopy.
A nondestructive protocol for preparing specimens of Monogenoidea for both alpha-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy.
Galli
Paolo
P
Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
Strona
Giovanni
G
Villa
Anna Maria
AM
Benzoni
Francesca
F
Fabrizio
Stefani
S
Doglia
Silvia Maria
SM
Kritsky
Delane C
DC
D000818:Animals; D002347:Carps / Q000469:parasitology*; D005393:Fish Diseases / Q000469:parasitology*; D018613:Microscopy, Confocal / Q000662:veterinary*; D010473:Perciformes / Q000469:parasitology*; D010986:Platyhelminths / Q000033:anatomy & histology*; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*