Gene transfer in common carp (Cyprinus carpio L.) by microinjection into the germinal disc.
We report here on the microinjection of the human growth hormone gene (hGH) into the germinal disc of common carp one-cell embryos. For microinjection, a linear 4.1 kb EcoRi-DNA fragment containing the mouse metallothionein-I promoter (mMT-I), fused to a structural gene coding for the hGH, was used. Of the 14 injected embryos, 9 developed to an age of 60 d. Of these nine fish, two were found to be transgenic and had integrated two to four copies per cell of the hGH construct.
Hernández
O
O
Mammalian Cell Genetics Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana, Cuba.
Castro
F O
FO
Aguilar
A
A
Uliver
C
C
Pérez
A
A
Herrera
L
L
de la Fuente
J
J