title: The influence of different types of media supplement on the meiotic maturation of bovine oocytes in vitro.
abstract: This work was designed to evaluate the influence of different types of media supplements in the maturation of preovulatory oocytes in cattle. We studied 4 different supplements: serum from estrous cattle (ECS), serum from fetal calves (FCS), amniotic fluid from cattle (bAF) and follicular fluid from cattle (bFF). Preovulatory bovine oocytes recovered from ovaries of mature cows after slaughter were cultured for 24 h in TCM-199 medium containing 20% of one of these protein supplements. The percentages of oocyte maturation were significantly higher (P < 0.001) in ECS (78%), FCS (79%) and bAF (77%) than in bFF (52%). These data indicate that supplementation with ECS, FCS or bFF is adequate for maturation of bovine oocytes without addition of other compounds.
authors.affiliation: Departamento de Genética. Laboratorlo de Citogenética Facultad de Veterinaria de Córdoba 14005-Córdoba. España.
authors.forename: J M
authors.initials: JM
authors.lastname: Ocaña Quero
authors.forename: M
authors.initials: M
authors.lastname: Moreno Millán
authors.forename: M
authors.initials: M
authors.lastname: Valera Córdoba
authors.forename: A
authors.initials: A
authors.lastname: Rodero Franganillo