[Evaluation of the enzyme-linked immunosorbent assay for the serodiagnosis of tularemia].
The usefulness of the ELISA using as antigen prepared in our laboratory supernatant obtained after centrifugation of sonicated F. tularensis cell suspension was compared with the tube agglutination test with commercial available antigen. Paired serum specimens obtained from 6 patients with ulceroglandular syndrome of tularemia were tested in both tests. The cut-off limit of serum antibodies was set at mean antibody titre determined in the sera of 115 blood donors exceeded by three standard deviations. Antibodies to F. tularensis in diagnostically significant titre were detected in all 12 serum samples by both tests. However the titres obtained in ELISA were several times higher than in tube agglutination test. In the second serum sample the level of IgA and IgM was lower but the level of IgG higher than in the first sample. We could not observe any difference in the level of antibodies between paired serum specimens in tube agglutination test.
Rastawicki
Waldemar
W
Zakład Bakteriologii PZH w Warszawie.
Jagielski
Marek
M
Gierczyński
Rafał
R
D000372:Agglutination Tests / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005604:Francisella tularensis / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D011237:Predictive Value of Tests; D012016:Reference Values; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D044967:Serum; D014406:Tularemia / Q000175:diagnosis* / Q000276:immunology
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M