title: Poxvirus CD8+ T-cell determinants and cross-reactivity in BALB/c mice.
abstract: Mouse models of orthopoxvirus disease provide great promise for probing basic questions regarding host responses to this group of pathogens, which includes the causative agents of monkeypox and smallpox. However, some essential tools for their study that are taken for granted with other mouse models are not available for these viruses. Here we map and characterize the initial CD8+ T-cell determinants for poxviruses in H-2d-haplotype mice. CD8+ T cells recognizing these three determinants make up around 40% of the total responses to vaccinia virus during and after resolution of infection. We then use these determinants to test if predicted conservation across orthopoxvirus species matches experimental observation and find an unexpectedly cross-reactive variant peptide encoded by ectromelia (mousepox) virus.
authors.affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases/NIH, Bethesda, MD 20892, USA. david.tscharke@anu.edu.au
authors.forename: David C
authors.initials: DC
authors.lastname: Tscharke
authors.forename: Wai-Ping
authors.initials: WP
authors.lastname: Woo
authors.forename: Isaac G
authors.initials: IG
authors.lastname: Sakala
authors.forename: John
authors.initials: J
authors.lastname: Sidney
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sette
authors.forename: Denis J
authors.initials: DJ
authors.lastname: Moss
authors.forename: Jack R
authors.initials: JR
authors.lastname: Bennink
authors.forename: Gunasegaran
authors.initials: G
authors.lastname: Karupiah
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Yewdell
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions / Q000276:immunology; D004195:Disease Models, Animal; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006239:Haplotypes / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018155:Orthopoxvirus / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D011213:Poxviridae Infections / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000956:Antigens, Viral; D018984:Epitopes, T-Lymphocyte; D010455:Peptides