title: [Epstein-Barr virus (EBV) infections in patients treated with allogenic hematopoietic cells transplantation (allo-HCT)].
abstract: OBJECTIVE
  Assessment of frequency and clinical course of EBV infection in patients that underwent non-manipulated allo-HCT from matched-related donors.
  
  
  METHODS
  Active EBV infection was confirmed based on the presence of anti-EA antibodies (ELISA) and/or viral DNA (nPCR) isolated from peripheral leukocytes. For positive DNA-isolations semi-quantitative analysis were done. Patients were examined repeatedly, the time of monitoring was approximately 6 +/- 5 months.
  
  
  RESULTS
  Active EBV infection was confirmed in 27 among 56 examined allo-HCT recipients. Primary infection was detected in 5 patients, in the remaining patients it was probably the result of virus reactivation. In most cases EBV-load was approximately 200 copies per 1 million of leukocytes, 1 patient with lymphoproliferative disorder (PTLD) had 2 million copies. EBV infection was asymptomatic in most cases (17), in 7 cases aminotransferase levels were insignificantly increased, in 2--diarrhea was observed and in 4 patients GvHD was intensified.
  
  
  CONCLUSIONS
  In recipients without risk of PTLD, permanent monitoring of the EBV-load has no clinical justification.
authors.affiliation: Zakład Wirusologii Katedry Mikrobiologii CM UJ w Krakowie. mbzawili@cm-uj.krakow.pl
authors.forename: Barbara
authors.initials: B
authors.lastname: Zawilińska
authors.forename: Beata
authors.initials: B
authors.lastname: Piatkowska-Jakubas
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kopeć
authors.forename: Ewa
authors.initials: E
authors.lastname: Daszkiewicz
authors.forename: Ewa
authors.initials: E
authors.lastname: Kleszcz
authors.forename: Sława
authors.initials: S
authors.lastname: Szostek
authors.forename: Patrycja
authors.initials: P
authors.lastname: Mensah-Glanowska
authors.forename: Dorota
authors.initials: D
authors.lastname: Hawrylecka
authors.forename: Aleksander
authors.initials: A
authors.lastname: Skotnicki
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis* / Q000821:virology*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000302:isolation & purification; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014184:Transplantation, Homologous; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens