Flow injection chemiluminescence determination of cefadroxil using potassium permanganate and formaldehyde system.
A simple, rapid and precise flow injection chemiluminescence (FI-CL) method is proposed for the determination of cefadroxil and is suitable for application to other antibiotics containing phenolic hydroxyl groups. A possible mechanism for this selectivity is suggested. The method is based on the CL-emitting reaction between cefadroxil and potassium permanganate in sulfuric acid medium, enhanced by formaldehyde (HCHO). Under the optimum conditions, calibration graphs over the ranges of 0.05-0.8 and 1.0-10.0 microg ml(-1) were obtained. The proposed method was successfully applied to the determination of cefadroxil in pharmaceutical formulations with no evidence of interference from common excipients. The detection limit (3sigma) of this method is 25 ng ml(-1) (6.9 x 10(-8) mol l(-1)). The relative standard deviation was less than 2% for 0.4 and 4.0 microg ml(-1) cefadroxil (n = 20). The sample throughput was found to be 120 h(-1).
Thongpoon
Chalermporn
C
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiand Mai 50200, Thailand.
Liawruangrath
Boonsom
B
Liawruangrath
Saisunee
S
Wheatley
R Alan
RA
Townshend
Alan
A
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002138:Calibration; D002434:Cefadroxil / Q000032:analysis*; D005079:Excipients; D017022:Flow Injection Analysis; D005557:Formaldehyde / Q000737:chemistry*; D008163:Luminescent Measurements; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000592:standards; D011196:Potassium Permanganate / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D005079:Excipients; D004364:Pharmaceutical Preparations; D011196:Potassium Permanganate; D005557:Formaldehyde; D002434:Cefadroxil