Solution-phase molecular-scale computation with deoxyribozyme-based logic gates and fluorescent readouts.
Recent development of solution-phase molecular-scale Boolean calculations using deoxyribozymes is potentially an important step toward the development of autonomous therapeutic and diagnostic devices. Here, the construction of basic YES, AND, ANDNOT, and ANDANDNOT deoxyribozyme-based logic gates is described. Protocols for testing gate activity using fluorescent oligonucleotide probes have been provided, and pointers for gate optimization are included.
Macdonald
Joanne
J
Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY, USA.
Stefanovic
Darko
D
Stojanovic
Milan N
MN
D001483:Base Sequence; D039301:Computers, Molecular; D021881:DNA, Catalytic; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes; D015335:Molecular Probes; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis*; D012235:Rhodamines
C114557:5-carboxytetramethylrhodamine succinimidyl ester; D021881:DNA, Catalytic; D005456:Fluorescent Dyes; D015335:Molecular Probes; D009841:Oligonucleotides; D012235:Rhodamines