[Linkage analysis of keloid susceptibility loci on chromosome 7p11 in a Chinese pedigree].
OBJECTIVE
To investigate whether the keloid susceptibility loci occur on chromosome 7p11 in a Chinese pedigree.


METHODS
Peripheral venous blood samples from 32 members significant for hereditary investigation from a large Chinese keloid pedigree (with 5 affected generations) were collected for extracting genomic DNA. Four microsatellites on chromosome 7p11 with known maximal two-point LOD scores were selected as the genetic markers according to the method described in a recent report of the similar study. These markers were subsequently amplified by PCR, and all the PCR products were genotyped and linkage analyses were conducted.


RESULTS
With the theta value of 0-0.1, the two-point LOD scores for these markers were all less than -2, which excluded the linkages of these markers to chromosome 7p11.


CONCLUSIONS
This study provides the first genetic evidence that keloid susceptibility loci in Chinese pedigree do not locate on chromosome 7p11, suggesting the heterogeneity of familial keloid susceptibility loci.
Chen
Yang
Y
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. cy_chen03@126.com
Gao
Jian-hua
JH
Liu
Xiao-jun
XJ
Yan
Xin
X
Song
Mei
M
D000293:Adolescent; D000328:Adult; D044466:Asian People / Q000235:genetics; D002681:China; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D006801:Humans; D007627:Keloid / Q000235:genetics*; D008126:Lod Score; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D010375:Pedigree