title: Purification, crystallization and preliminary X-ray crystallographic analysis of rice Bowman-Birk inhibitor from Oryza sativa.
abstract: Bowman-Birk inhibitors (BBIs) are cysteine-rich proteins with inhibitory activity against proteases that are widely distributed in monocot and dicot species. The expression of rice BBI from Oryza sativa is up-regulated and induced by pathogens or insects during germination of rice seeds. The rice BBI (RBTI) of molecular weight 15 kDa has been crystallized using the hanging-drop vapour-diffusion method. According to the diffraction of rice BBI crystals at a resolution of 2.07 A, the unit cell belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 74.37, b = 96.69, c = 100.36 A. Preliminary analysis indicates four BBI molecules in an asymmetric unit, with a solvent content of 58.29%.
authors.affiliation: Life Science Group, Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Yi-Hung
authors.initials: YH
authors.lastname: Lin
authors.forename: Hsin-Tai
authors.initials: HT
authors.lastname: Li
authors.forename: Yen-Chieh
authors.initials: YC
authors.lastname: Huang
authors.forename: Ying-Cheng
authors.initials: YC
authors.lastname: Hsieh
authors.forename: Hong-Hsiang
authors.initials: HH
authors.lastname: Guan
authors.forename: Ming-Yih
authors.initials: MY
authors.lastname: Liu
authors.forename: Tschining
authors.initials: T
authors.lastname: Chang
authors.forename: Andrew H-J
authors.initials: AH
authors.lastname: Wang
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D003460:Crystallization / Q000379:methods; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D011480:Protease Inhibitors / Q000737:chemistry*; D012997:Solvents; D014961:X-Ray Diffraction
chemical_list: D010940:Plant Proteins; D011480:Protease Inhibitors; D012997:Solvents