title: Crystallization and preliminary crystallographic analysis of orotidine 5'-monophosphate decarboxylase from the human malaria parasite Plasmodium falciparum.
abstract: Orotidine 5'-monophosphate (OMP) decarboxylase (OMPDC; EC 4.1.1.23) catalyzes the final step in the de novo synthesis of uridine 5'-monophosphate (UMP) and defects in the enzyme are lethal in the malaria parasite Plasmodium falciparum. Active recombinant P. falciparum OMPDC (PfOMPDC) was crystallized by the seeding method in a hanging drop using PEG 3000 as a precipitant. A complete set of diffraction data from a native crystal was collected to 2.7 A resolution at 100 K using synchrotron radiation at the Swiss Light Source. The crystal exhibits trigonal symmetry (space group R3), with hexagonal unit-cell parameters a = b = 201.81, c = 44.03 A. With a dimer in the asymmetric unit, the solvent content is 46% (V(M) = 2.3 A3 Da(-1)).
authors.affiliation: Unit of Biochemistry, Department of Medical Science, Faculty of Science, Rangsit University, Patumthani 12000, Thailand.
authors.forename: Sudaratana R
authors.initials: SR
authors.lastname: Krungkrai
authors.forename: Keiji
authors.initials: K
authors.lastname: Tokuoka
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kusakari
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Adachi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Takano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Murakami
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Mori
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kai
authors.forename: Jerapan
authors.initials: J
authors.lastname: Krungkrai
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Horii
mesh_terms: D000818:Animals; D003460:Crystallization; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000201:enzymology*; D011994:Recombinant Proteins; D012997:Solvents; D014961:X-Ray Diffraction
chemical_list: D011994:Recombinant Proteins; D012997:Solvents; D009964:Orotidine-5'-Phosphate Decarboxylase