Cloning of the promoter from the gonadal aromatase gene of the European sea bass and identification of single nucleotide polymorphisms.
Aromatase contributes to sex differentiation by catalysing the conversion of androgens into estrogens. We have cloned the promoter of the gonadal aromatase gene from the sea bass, Dicentrarchus labrax, a species whose farming is complicated by male-skewed sex ratios. The promoter shows a conserved binding site for SF-1 as well as two cAMP response elements (CREs) and putative binding sites for transcription factors belonging to the Sox and forkhead families. Analysis of promoter sequences from individual fish suggests the presence of three promoter alleles that arise due to three single nucleotide polymorphisms (SNPs) in linkage disequilibrium.
Galay-Burgos
Malyka
M
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, UK.
Gealy
Claire
C
Navarro-Martín
Laia
L
Piferrer
Francesc
F
Zanuy
Silvia
S
Sweeney
Glen E
GE
D000483:Alleles; D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001483:Base Sequence; D001492:Bass / Q000235:genetics*; D003001:Cloning, Molecular; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D006066:Gonads / Q000201:enzymology* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D054339:Steroidogenic Factor 1; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D018398:Homeodomain Proteins; D009841:Oligonucleotides; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D014157:Transcription Factors; D001141:Aromatase