[PPARgamma1 overexpression on caveolin-1 expression of Raw264.7 cells].
OBJECTIVE
To investigate the effect of PPARgamma1 gene overexpression on caveolin-1 mRNA and protein expressions in a murine macrophage cell line Raw264.7.


METHODS
Replication-deficient recombinant adenovirus expression vector of PPARgamma1 was constructed using the AdEasy system. Raw264.7 cells were randomly treated as follows: P group (PPARgamma1 gene overexpression), T group (Troglitazone 40 micromol/L in DMSO), PT group (PPARgamma1 gene overexpression and Troglitazone) and control group. Changes of PPARgamma1 and caveolin-1 at mRNA and protein levels were investigated.


RESULTS
Caveolin-1 expression can be detected by RT-PCR in Raw264.7, by immunocytochemistry method in cell and nuclear membrane but not by immunoblotting at protein level. Caveolin-1 expression at mRNA and protein levels in Raw264.7 were significantly higher in P, T and PT groups compared to control group and the expression was also significantly higher in PT group than that in P group and T group (P < 0.05). PPARgamma expression was significantly increased in PT group and P group where remained unchanged in T group compared to control group.


CONCLUSIONS
PPARgamma1 overexpression can upregulate caveolin-1 expression in macrophages. Troglitazone upregulated caveolin-1 expression in the absence of increased PPARgamma1 expressions at mRNA and protein levels.
Hu
Qin
Q
Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital, Shandong University, Jinan 250012, China.
Zhang
Yun
Y
Liu
Chun-xi
CX
Zhang
Mei
M
Jing
Ma
M
He
Hong
H
Feng
Jin-bo
JB
Wang
Rong
R
Jiang
Gui-hua
GH
Zhang
Xian-jun
XJ
Jiang
Hong
H
Zhu
Qing
Q
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D051242:Caveolin 1 / Q000378:metabolism*; D002460:Cell Line; D002839:Chromans / Q000494:pharmacology; D015870:Gene Expression; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D047495:PPAR gamma / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D045162:Thiazolidinediones / Q000494:pharmacology; D000077288:Troglitazone
D051242:Caveolin 1; D002839:Chromans; D047495:PPAR gamma; D012333:RNA, Messenger; D045162:Thiazolidinediones; D000077288:Troglitazone