Low dose radiation overcomes diabetes-induced suppression of hippocampal neuronal cell proliferation in rats.
We investigated the effect of low dose radiation on diabetes induced suppression of neurogenesis in the hippocampal dentate gyrus of rat. After 0.01 Gy, 0.1 Gy, 1 Gy and 10 Gy radiation was delivered, the dentate gyrus of hippocampus of streptozotocin (STZ)-induced diabetic rats were evaluated using immunohistochemistry for 5-bromo-2-deoxyuridine (BrdU), caspase-3, and terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL) staining. The number of BrdU positive cells in the non-diabetic rats, diabetic rats without radiation, diabetic rats with 0.01 Gy radiation, diabetic rats with 0.1 Gy radiation, diabetic rats with 1 Gy radiation and diabetic rats with 10 Gy radiation were 55.4+/-8.5/mm2, 33.3+/-6.4/mm2, 67.7+/-10.5/mm2, 66.6+/-10.0/mm2, 23.5+/-6.3/mm2 and 14.3+/-7.2/mm2, respectively. The number of caspase-3 positive cells was 132.6+/-37.4/mm2, 378.6+/-99.1/mm2, 15.0+/-2.8/mm2, 57.1+/-16.9/mm2, 191.8+/-44.8/mm2 and 450.4+/-58.3/mm2, respectively. The number of TUNEL-positive cells was 24.5+/-2.0/mm2, 21.7+/-4.0/mm2, 20.4+/-2.0/mm2, 18.96+/-2.1/mm2, 58.3+/-7.9/mm2, and 106.0+/-9.8/mm2, respectively. These results suggest low doses of radiation paradoxically improved diabetes induced neuronal cell suppression in the hippocampal dentate gyrus of rat.
Kang
Jin Oh
JO
Department of Radiation Oncology, School of Medicine, Kyung Hee University, Seoul, Korea. kangjino@khmc.or.kr
Kim
Sang Ki
SK
Hong
Seong Eon
SE
Lee
Taeck Hyun
TH
Kim
Chang Ju
CJ
D000818:Animals; D017209:Apoptosis; D001973:Bromodeoxyuridine / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000187:drug effects / Q000528:radiation effects*; D003921:Diabetes Mellitus, Experimental / Q000532:radiotherapy; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects; D020287:In Situ Nick-End Labeling; D008297:Male; D009474:Neurons / Q000378:metabolism*; D011878:Radiotherapy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
D053148:Caspase 3; D001973:Bromodeoxyuridine