Uniconazole-induced tolerance of soybean to water deficit stress in relation to changes in photosynthesis, hormones and antioxidant system.
This study investigated whether uniconazole confers drought tolerance to soybean and if such tolerance is correlated with changes in photosynthesis, hormones and antioxidant system of leaves. Soybean plants were foliar treated with uniconazole at 50 mg L-1 at the beginning of bloom and then exposed to water deficit stress at pod initiation for 7 d. Uniconazole promoted biomass accumulation and seed yield under both water conditions. Plants treated with uniconazole showed higher leaf water potential only in water-stressed condition. Water stress decreased the chlorophyll content and photosynthetic rate, but those of uniconazole-treated plants were higher than the stressed control. Uniconazole increased the maximum quantum yield of photosystemand ribulose-1,5-bisphosphate carboxylase/oxygenase activity of water-stressed plants. Water stress decreased partitioning of assimilated 14C from labeled leaf to the other parts of the plant. In contrast, uniconazole enhanced translocation of assimilated 14C from labeled leaves to the other parts, except stems, regardless of water treatment. Uniconazole-treated plants contained less GA3, GA4 and ABA under well-watered condition than untreated plants, while the IAA and zeatin levels were increased substantially under both water conditions, and ABA concentration was also increased under water stressed condition. Under water-stressed conditions, uniconazole increased the content of proline and soluble sugars, and the activities of superoxide dismutase and peroxidase in soybean leaves but not the malondialdehyde content or electrical conductivity. These results suggest that uniconazole-induced tolerance to water deficit stress in soybean was related to the changes of photosynthesis, hormones and antioxidant system of leaves.
Zhang
Mingcai
M
State Key Laboratory of Plant Physiology and Biochemistry, Department of Agronomy, Centre for Crop Chemical Control, College of Agronomy and Biotechnology, China Agricultural University, 2#, Yuanmingyuan Xilu, Haidian District, Beijing, 100094, PR China.
Duan
Liusheng
L
Tian
Xiaoli
X
He
Zhongpei
Z
Li
Jianmin
J
Wang
Baomin
B
Li
Zhaohu
Z
D000040:Abscisic Acid / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002244:Carbon / Q000378:metabolism; D002250:Carbon Radioisotopes; D002734:Chlorophyll / Q000378:metabolism; D004553:Electric Conductivity; D005875:Gibberellins / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects*; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D014230:Triazoles / Q000494:pharmacology*; D014867:Water / Q000378:metabolism*
D000975:Antioxidants; D002250:Carbon Radioisotopes; D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014230:Triazoles; D014867:Water; D002734:Chlorophyll; D000040:Abscisic Acid; D002244:Carbon; D011392:Proline; C007842:gibberellic acid; D012273:Ribulose-Bisphosphate Carboxylase; C092705:uniconazole