title: [Relationship between length of sleep and oxidative stress marker, urinary 8-hydroxy-2'-deoxyguanosine].
abstract: OBJECTIVE
  In this study, we aim to examine whether the length of sleep modifies the level of urinary 8-hydroxy-2'-deoxyguanosine (8-OH-dG).
  
  
  METHODS
  Subjects were 146 workers who were engaged in management, clerical work, laboratory work, or business at a certain company. We obtained information on the subjects concerning gender, body mass index, drinking and smoking habits, and the lengths of habitual and previous night sleep among others using self-reported questionnaires, which were then confirmed by interview. Urine specimens were collected in the morning and those from the second or later void were used to measure 8-OH-dG and creatinine levels. The amount of 8-OH-dG normalized by creatinine content was used as the indicator of 8-OH-dG excretion (Spot Urine 8-OH-dG: SU8-OH-dG). We excluded subjects who took sleeping pills the previous night or habitually, and those whose levels were beyond the three-standard-deviation range. The subjects were then classified into three equal-sized groups according to the length of sleep, either habitual or the previous night.
  
  
  CONCLUSIONS
  (1) A long or a short previous night's sleep, drinking alcohol more than once a week, and habitual use of medicine increased SU8-OH-dG level among female subjects. (2) A short previous night's sleep and habitual smoking, and a short previous night's sleep and habitual use of medicine had interactions which increased SU8-OH-dG level among male subjects but the length of a previous night's sleep itself did not have an effect on SU8-OH-dG level. (3) The length of habitual sleep had no effect on SU8-OH-dG level.
authors.affiliation: National Institute of Industrial Health, Kawasaki.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Rie
authors.initials: R
authors.lastname: Yoshida
authors.forename: Akinori
authors.initials: A
authors.lastname: Nakata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kasai
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ohba
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Ohta
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D008019:Life Style; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D012044:Regression Analysis; D012890:Sleep / Q000502:physiology*; D011795:Surveys and Questionnaires; D013997:Time Factors
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine