Blinded comparison of repetitive-sequence PCR and multilocus sequence typing for genotyping methicillin-resistant Staphylococcus aureus isolates from a children's hospital in St. Louis, Missouri.
We performed a blinded study to compare repetitive-sequence PCR and multilocus sequence typing for genotyping hospital- and community-acquired methicillin-resistant Staphylococcus aureus (MRSA). The MRSA strains that were sequence type 8 (ST8), staphylococcal cassette chromosome mec (SCCmec) type IV, and Panton-Valentine leukocidin-positive clustered separately from those that were ST5 and SCCmec type II.
Liao
Robert S
RS
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Storch
Gregory A
GA
Buller
Richard S
RS
Orscheln
Rachel C
RC
Mardis
Elaine R
ER
Armstrong
Jon R
JR
Dunne
W Michael
WM
D000293:Adolescent; D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000382:microbiology; D003428:Cross Infection / Q000382:microbiology; D005838:Genotype; D006776:Hospitals, Pediatric; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D008923:Missouri; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics