title: Antithrombotic activities of aqueous extract from Radix Ophiopogon japonicus and its two constituents.
abstract: To provide further pharmacological evidence for its clinical use in thrombotic diseases, the antithrombotic activities of the aqueous extract of Radix Ophiopogon japonicus (ROJ-ext) were studied in mouse and rat models. The results showed that ROJ-ext remarkably decreased length of tail thrombus in mice at 48 h and 72 h after carrageenan injection at doses of 12.5 and 25.0 mg/kg. Meanwhile, ROJ-ext markedly inhibited thrombosis induced by arterial-venous (AV) shunt (silk thread) in rats at doses of 6.25 and 12.5 mg/kg. Furthermore, ROJ-ext and one of its components, ruscogenin, significantly inhibited platelet aggregation induced by adenosine diphosphate (ADP) in rats by oral administration of 12.5 mg/kg or 0.7 mg/kg for three times, however, ophiopogonin D 1.4 mg/kg only showed slight inhibition. On the other hand, ophiopogonin D (0.5-2.0 mg/kg, p.o.) and ruscogenin (0.25-1.00 mg/kg, p.o.) produced dose-related inhibition of venous thrombosis induced by tight ligation of the inferior vena cava for 6 h in mice by once oral administration. The findings of this study indicate that an aqueous extract of Radix Ophiopogon japonicus (ROJ-ext) exerted significant antithrombotic activity and ruscogenin and ophiopogonin D are two of its active components, which supported its therapeutic use for thrombotic diseases.
authors.affiliation: Department of Traditional Chinese Prescription, School of Chinese Materia Medica, China Pharmaceutical University, Nanjing, P.R. China.
authors.forename: Junping
authors.initials: J
authors.lastname: Kou
authors.forename: Youqing
authors.initials: Y
authors.lastname: Tian
authors.forename: Yunkit
authors.initials: Y
authors.lastname: Tang
authors.forename: Jin
authors.initials: J
authors.lastname: Yan
authors.forename: Boyang
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D002351:Carrageenan; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005343:Fibrinolytic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D031441:Ophiopogon / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020246:Venous Thrombosis / Q000097:blood / Q000188:drug therapy*
chemical_list: D004365:Drugs, Chinese Herbal; D005343:Fibrinolytic Agents; D002351:Carrageenan