[Cloning, expression and subcellular localization of a novel human gene-RNF122].
OBJECTIVE
To clone a functionally unknown gene RNF122 and analyze its expression pattern and subcellular localization.


METHODS
PCR was used to clone the novel gene-RNF122 from the mixed human tissue cDNA library. Bioinformatics analysis was used to identify structure characteristics of the gene. Northern blot was used to analyze its expression in normal tissues; RT-PCR was employed for its expression in cell lines and tumor tissues. By co-focal microscope, we identified its subcellular localization with organelle markers.


RESULTS
A novel human gene-RNF122 was cloned, which was proved to have been expressed in several normal and tumor tissues and many cell lines, and localized in ER and Golgi apparatus.


CONCLUSIONS
RNF122 is a novel gene which encodes a protein that has a classic RING domain. It is widely expressed in several tissues and cell lines. The encoded protein is localized in ER and Golgi apparatus, which indicates that it may play a role in the process of protein degradation.
Wang
Li
L
Department of Immunology, Peking University School of Basic Medical Science Center for Human Disease Genomics, Peking University, Beijing 100083, China.
Shi
Tai-ping
TP
Wang
Lan
L
Yu
Chuan-fei
CF
Zeng
Ling-e
LE
Wang
Jing
J
Wang
Lu
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015870:Gene Expression; D006056:Golgi Apparatus / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C529404:ring finger protein 122, human; D049452:Green Fluorescent Proteins