Omental and subcutaneous adipose tissue steroid levels in obese men.
We examined plasma and fat tissue sex steroid levels in a sample of 28 men aged 24.8-62.2 years (average BMI value of 46.3 +/- 12.7 kg/m(2)). Abdominal adipose tissue biopsies were obtained during general or obesity surgery. Omental and subcutaneous adipose tissue steroid levels were measured by gas chromatography and chemical ionization mass spectrometry after appropriate extraction procedures. BMI and waist circumference were negatively correlated with plasma testosterone (r = -0.49 and -0.50, respectively, p < 0.01) and dihydrotestosterone (r = -0.58 and -0.56, respectively, p < 0.01), and positively associated with estrone levels (r = 0.64 and 0.62, respectively, p < 0.001). Regional differences in adipose tissue steroid levels were observed for dihydrotestosterone (p < 0.005), androstenedione (p < 0.0001) and dehydroepiandrosterone levels (p < 0.05), which were all significantly more concentrated in omental versus subcutaneous fat. Positive significant associations were found between circulating level of a steroid and its concentration in omental and subcutaneous adipose tissue, for estrone (r = 0.72 and 0.57, respectively, p < 0.01), testosterone (r = 0.66 and 0.58, respectively, p < 0.01) and dihydrotestosterone (r = 0.58 and 0.45, respectively, p < 0.05). Positive correlations were observed between plasma dehydroepiandrosterone-sulfate and omental (r = 0.56, p < 0.01) as well as subcutaneous adipose tissue dehydroepiandrosterone level (r = 0.38, p = 0.05). Positive significant associations were found between omental adipocyte responsiveness to positive lipolytic stimuli (isoproterenol, dibutyryl cyclic AMP and forskolin) and plasma or omental fat tissue androgen levels. In conclusion, although plasma androgen or estrogen levels are strong correlates of adipose tissue steroid content both in the omental and subcutaneous fat depots, regional differences may be observed. Androgen concentration differences in omental versus subcutaneous adipose tissue suggest a depot-specific impact of these hormones on adipocyte function and metabolism.
Bélanger
Chantal
C
Molecular Endocrinology and Oncology Research Center, Laval University, Canada.
Hould
Frédéric-Simon
FS
Lebel
Stéfane
S
Biron
Simon
S
Brochu
Gaétan
G
Tchernof
André
A
D050153:Abdominal Fat / Q000737:chemistry*; D017667:Adipocytes / Q000378:metabolism; D000328:Adult; D050218:Body Fat Distribution; D015992:Body Mass Index; D001837:Body Weights and Measures; D012739:Gonadal Steroid Hormones / Q000032:analysis / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000473:pathology* / Q000601:surgery; D009852:Omentum / Q000737:chemistry*; D013256:Steroids / Q000032:analysis* / Q000097:blood; D050151:Subcutaneous Fat / Q000737:chemistry*; D014018:Tissue Distribution
D012739:Gonadal Steroid Hormones; D013256:Steroids