A microfluidic fluorous solid-phase extraction chip for purification of amino acids.
An electrokinetically-driven microfluidic chip was developed to realize beads-based solid-phase extraction (SPE) of amino acids. This chip uses a two-level (deep/shallow) poly(dimethylsiloxane) (PDMS) microchannel network to confine the fluorous reversed-phase silica beads within the SPE chamber. The mixture of fluorous tagged and non-tagged amino acids was carried into the fluorous solid-phase extraction (F-SPE) chamber by electrokinetic pumping and was successfully separated and extracted. By adding a reference material to the sample, the extraction efficiency of the eluted fluorous-tagged amino acid was calculated using the detection results from mass spectrometry (MS). The F-SPE microchips showed good reproducibility and efficiency, yielding an average extraction efficiency of 55% with a RSD of 10.6% under the typical experimental conditions.
Hu
Guoqing
G
Department of Mechanical Engineering, Vanderbilt University, VU Station B 351592, 2301 Vanderbilt Place, Nashville, TN 37235-1592, USA.
Lee
Jacky S H
JS
Li
Dongqing
D
D000596:Amino Acids / Q000737:chemistry* / Q000302:isolation & purification; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D044085:Microfluidics; D012828:Silicones / Q000737:chemistry; D052616:Solid Phase Extraction
D000596:Amino Acids; D004129:Dimethylpolysiloxanes; D006845:Hydrocarbons, Fluorinated; D012828:Silicones; C013830:baysilon