B cells in murine cervical lymph nodes are conventional B-2 cells.
We investigated the characteristic features of cervical lymph node B cells to determine whether their behavior differs from that of B cells located elsewhere, because cervical lymph nodes may be exposed to continual antigenic stimulation from the naso- and/or oropharynx. B cells were isolated from cervical lymph nodes, spleen and peritoneal fluid of mice, cultured in medium, and exposed to various stimuli. The expression of various surface molecules characteristic of lymphoid B cells was assayed by flow cytometry, and immunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent assay. B220+ cells were cultured in medium alone or with lipopolysaccharide, and their entrance into S phase in response to stimuli was measured by proliferative assays. Phenotypic characteristics of cervical lymph node B cells included CD5 low, CD23high, CD43low, B7.1low, B7.2low, and Syndecan-1low. Unstimulated lymphoid B cells did not secrete immunoglobulin, but, upon stimulation, secretion of IgM was increased more than secretion of IgA and IgG. B cells actively entered S phase after 48 hr stimulation. These results show that B cells in cervical lymph nodes are conventional B2 cells, like splenic B cells.
Yeo
Seung Geun
SG
Department of Otolaryngology, College of Medicine, Kyung Hee University, Seoul, Korea.
Cho
Joong Saeng
JS
Park
Dong Choon
DC
D000818:Animals; D000941:Antigens / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D003470:Culture Media / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007075:Immunoglobulin M / Q000737:chemistry; D008198:Lymph Nodes / Q000166:cytology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D013154:Spleen / Q000166:cytology
D000941:Antigens; D003470:Culture Media; D007075:Immunoglobulin M