Delivery of nitric oxide released from beta-Gal-NONOate activation by beta-galactosidase and its activity against Escherichia coli.
Beta-galactosyl-pyrrolidinyl diazeniumdiolates (beta-Gal-NONOate) is a new site-specific nitric oxide (NO)-releasing compound, which releases NO once activated by beta-galactosidase. In the present experiment, we used beta-Gal-NONOate as a bactericidal reagent to investigate its effectiveness of NO releasing. Through the evaluation of intracellular NO level and the comparison of survival of E. coli transformed with lacZ gene but treated with beta-Gal-NONOate and NONOate, respectively, it's evident that beta-Gal-NONOate had a higher bactericidal activity than conventional NONOate. While either beta-Gal-NONOate- or NONOate-treated E. coli without transferred lacZ gene showed low bactericidal activity. The results revealed that beta-Gal-NONOate was a potentially efficient NO donor, which took on a novel and promising approach to deliver NO into cells.
Chen
Chang
C
State Key Laboratory of Microbial Technology (SKLMT), Shandong University, Jinan, Shandong, China.
Shi
Yan-Qiu
YQ
Song
Jing
J
Qi
Qing-Sheng
QS
Gu
Li
L
Wang
Peng George
PG
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001391:Azo Compounds / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D005690:Galactose / Q000031:analogs & derivatives* / Q000494:pharmacology; D050296:Microbial Viability; D009569:Nitric Oxide / Q000378:metabolism* / Q000502:physiology; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001391:Azo Compounds; D020030:Nitric Oxide Donors; C514571:beta-Gal-NONOate; D009569:Nitric Oxide; D001616:beta-Galactosidase; D005690:Galactose