Fluorescent aromatic platforms for cell patterning.
This paper describes a simple experimental method of patterning fluorescent organic dyes, fluorescein, and rhodamine on gold substrates by microcontact printing techniques. The development of this step-by-step protocol has allowed us to prepare striped and squared micropatterns with poly(ethylene glycol) (PEG) linkers terminated by these fluorophores using a fast, easy, and inexpensive technique. Although the rest of the surface was covered with aliphatic molecules (methyl terminated), human fibroblasts demonstrated an unexpected response, aligning themselves according to the aromatic patterns, despite the presence of PEG, which is a cell resistant molecule, in the fluorescent regions.
de la Fuente
Jesus M
JM
Centre for Cell Engineering, Institute of Biomedical and Life Science, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK. lafuente@iiq.csic.es
Andar
Abhay
A
Gadegaard
Nikolaj
N
Berry
Catherine C
CC
Kingshott
Peter
P
Riehle
Mathis O
MO
D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure
D020099:Coated Materials, Biocompatible; D005456:Fluorescent Dyes