A colorimetric assay for screening transketolase activity.
A tetrazolium red-based colorimetric assay has been devised to screen for transketolase activity with a range of aldehyde acceptors. The colorimetric TK assay is able to detect >8% bioconversion using non-alpha-hydroxylated aldehydes as acceptor substrates and is significantly faster and more convenient to use than chromatographic procedures.
Smith
Mark E B
ME
Department of Chemistry, University College London, 20 Gordon Street, London WC1H OAJ, UK.
Kaulmann
Ursula
U
Ward
John M
JM
Hailes
Helen C
HC
D000447:Aldehydes / Q000737:chemistry; D002384:Catalysis; D003124:Colorimetry / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004789:Enzyme Activation; D007659:Ketones / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D013778:Tetrazolium Salts / Q000737:chemistry; D014174:Transketolase / Q000737:chemistry*
D000447:Aldehydes; D007659:Ketones; D013778:Tetrazolium Salts; D014174:Transketolase