title: Isolation of marine bacteria by in situ culture on media-supplemented polyurethane foam.
abstract: Polyurethane foam (PUF) supplemented with various agar media was used in situ to trap marine bacteria and it consequently provided a substrate on which they could be cultivated while exposed to natural seawater in the coral reef area. The bacterial population on the PUF blocks was analyzed by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 16S rDNA fragments. Changing the composition of the cultivation medium in the PUF blocks and selecting different sampling sites resulted in different bacteria being detected on the PUF blocks. For example, iron-utilizing (IU) bacteria, siderophore-producing (SP) bacteria, and petroleum-degrading (PD) bacteria were isolated from PUF blocks and it was discovered that IU and SP contained iron and PD contained hydrocarbon. This method opens up the possibility for isolating novel and useful marine bacteria.
authors.affiliation: Marine Biotechnology Institute (MBI), 3-75-1 Heita, Kamaishi-shi, Iwate, 026-0001, Japan.
authors.forename: Mina
authors.initials: M
authors.lastname: Yasumoto-Hirose
authors.forename: Miyuki
authors.initials: M
authors.lastname: Nishijima
authors.forename: Metiek Kimie
authors.initials: MK
authors.lastname: Ngirchechol
authors.forename: Kaneo
authors.initials: K
authors.lastname: Kanoh
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Shizuri
authors.forename: Wataru
authors.initials: W
authors.lastname: Miki
mesh_terms: D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D001431:Bacteriological Techniques; D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D010578:Petroleum; D011140:Polyurethanes / Q000737:chemistry*; D012623:Seawater; D017262:Siderophores / Q000378:metabolism
chemical_list: D003470:Culture Media; D010578:Petroleum; D011140:Polyurethanes; D017262:Siderophores