Purification and characterization of a heat-stable serine protease inhibitor from the tubers of new potato variety "Golden Valley".
Potide-G, a small (5578.9 Da) antimicrobial peptide, was isolated from potato tubers (Solanum tuberosum L. cv. Golden Valley) through extraction of the water-soluble fraction, dialysis, ultrafiltration and DEAE-cellulose and C18 reverse-phase high performance liquid chromatography. This antimicrobial peptide was heat-stable and almost completely suppressed the proteolytic activity of trypsin, chymotrypsin and papain, with no hemolytic activity. In addition, potide-G potently inhibited growth of a variety of bacterial (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, and Clavibacter michiganense subsp. michiganinse) and fungal (Candida albicans and Rhizoctonia solani) strains. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry revealed that the N-terminal sequence (residues from 1 to 11) of the protein is identical to that of potato proteinase inhibitor, a member of the Kunitz superfamily. And like other members of this class of protease inhibitor, potide-G may have a number of beneficial and therapeutic uses.
Kim
Mi-Hyun
MH
Research Center for Proteineous Materials, Chosun University, Kwangju 501-759, Republic of Korea.
Park
Seong-Cheol
SC
Kim
Jin-Young
JY
Lee
Sun Young
SY
Lim
Hak-Tae
HT
Cheong
Hyeonsook
H
Hahm
Kyung-Soo
KS
Park
Yoonkyung
Y
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D035281:Plant Tubers / Q000737:chemistry*; D016415:Sequence Alignment; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011198:Solanum tuberosum / Q000737:chemistry* / Q000145:classification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010940:Plant Proteins; D015842:Serine Proteinase Inhibitors