Effect of methamidophos and urea application on microbial communities in soils as determined by microbial biomass and community level physiological profiles.
In this study, we evaluated the effect of the application by two agrochemicals, methamidophos (O,S-dimethyl phosphoroamidothioate) and urea, on microbial diversity in soil, using the combined approaches of soil microbial biomass analysis and community level physiological profiles (CLPPs). The results showed that both a low and a high level of methamidophos application (CS2 and CS3) and urea application (CS4) significantly decreased microbial biomass C (Cmic) by 41-83% compared with the control (CS1). The soil organic C (Corg) values of CS3 and CS4 were significantly higher and lower by 24% and 14%, respectively, than that of CS1. Similarly to Cmic, the values of Cmic/Corg of the three applied soils which decreased were lower by 31-84% than that of CS1. In contrast, the respiration activity of the three applied soils were significantly higher than the control. Agrochemical application also significantly increased the soil total of N and P (Ntol and Ptol) and decreased the Corg/Ntol and Corg/Ptol values. The CLPPs results showed that the AWCD (average well color development) of the three applied soils were significantly higher than that of CS1 during the incubation period. Substrate richness, Shannon and Simpson indices of microbial communities under chemical stresses, increased significantly. In addition, the CFU (colony-forming unit) numbers of methamidophos metabolized bacteria in CS2 and CS3 also increased significantly by 86.1% and 188.9% compared with that of CS1. The combined results suggest that agrochemicals reduce microbial biomass and enhance functional diversities of soil microbial communities; meanwhile, some species of bacteria may be enriched in soils under methamidophos stress.
Wang
Meng-Cheng
MC
State Key Laboratory of Pharmaceutical Biotechnology, Plant Cell and Molecular Biology Laboratory, Department of Biological Science and Technology, School of Life Science, Nanjing University, Nanjing, P.R. China.
Gong
Ming
M
Zang
Hong-Bing
HB
Hua
Xiao-Mei
XM
Yao
Jian
J
Pang
Yan-Jun
YJ
Yang
Yong-Hua
YH
D018533:Biomass; D002244:Carbon / Q000032:analysis; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring; D007306:Insecticides / Q000494:pharmacology*; D009584:Nitrogen / Q000032:analysis; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*; D010758:Phosphorus / Q000032:analysis; D011157:Population Dynamics; D012988:Soil Microbiology; D013045:Species Specificity; D014508:Urea / Q000494:pharmacology*
D007306:Insecticides; D009946:Organothiophosphorus Compounds; D010758:Phosphorus; D002244:Carbon; D014508:Urea; C014655:methamidophos; D009584:Nitrogen