A highly restricted T-cell receptor dominates the CD8+ T-cell response to parvovirus B19 infection in HLA-A*2402-positive individuals.
Six of seven HLA-A*2402-positive individuals with acute parvovirus B19 infections made vigorous CD8-positive cytotoxic T-cell (CTL) responses to the viral epitope FYTPLADQF. All responders showed highly focused T-cell receptor (TCR) usage, using almost exclusively BV5.1. The BV5.1 TCR dominated the acute response, was maintained over time, and was also used by a remotely infected individual. Nine CTL clones and two oligoclonal lines obtained from three unrelated individuals used BV5.1, BJ2.1, and a conserved TCR CDR3 of nine amino acids. This commonly recognized epitope is likely important in long-term protective immunity and should be included in vaccine design.
Kasprowicz
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V
Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA. vkasprowicz@partners.org
Isa
A
A
Jeffery
K
K
Broliden
K
K
Tolfvenstam
T
T
Klenerman
P
P
Bowness
P
P
D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D022801:Complementarity Determining Regions / Q000235:genetics / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D015234:HLA-A Antigens / Q000235:genetics / Q000276:immunology*; D059871:HLA-A24 Antigen; D006801:Humans; D008297:Male; D010322:Parvoviridae Infections / Q000235:genetics / Q000276:immunology*; D016732:Parvovirus B19, Human / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
D022801:Complementarity Determining Regions; D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens; C438506:HLA-A*24:02 antigen; D059871:HLA-A24 Antigen; D014765:Viral Vaccines