The use of combined immunohistochemical labeling and in situ hybridization to colocalize mRNA and protein in tissue sections.
This chapter explores the combination of a nonradioactive in situ hybridization technique to detect mRNA with an imunnohistochemical labeling technique for use in formalin-fixed, paraffin-embedded, or frozen tissue sections. This technique allows the combination of detecting mRNA by in situ hybridization with immunohistochemical detection of a protein product or a cell surface marker without using any radioactive procedures. This technique is ideal for use on tissue sections when the aim is to identify which cells are producing a secreted protein product, such as a cytokine.
Smith
Malcolm D
MD
Rheumatology Unit, Repatriation General Hospital, Daw Park, Adelaide, Australia.
Ahern
Michael
M
Coleman
Mark
M
D005404:Fixatives; D005557:Formaldehyde; D005629:Frozen Sections; D006652:Histological Techniques; D007150:Immunohistochemistry / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D016612:Paraffin Embedding; D011506:Proteins / Q000145:classification / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*
D005404:Fixatives; D011506:Proteins; D012333:RNA, Messenger; D005557:Formaldehyde