title: Resuscitation of severe but brief haemorrhagic shock with PFC in rabbits restores skeletal muscle oxygen delivery and does not alter skeletal muscle metabolism.
abstract: Studies have demonstrated that perfluorocarbon (PFC) emulsions associated with hyperoxia improved whole body oxygen delivery during resuscitation of acute haemorrhagic shock (HS). Nevertheless the microcirculatory effects of PFC and the potential deleterious effects of hyperoxic reperfusion are still of concern. We investigated (i) the ability of a newly formulated, small sized and highly stable PFC emulsion to increase skeletal muscle oxygen delivery and (ii) the effect of hyperoxic reperfusion on skeletal muscle metabolism after a brief period of ischaemia using an original, microdialysis-based method that allowed simultaneous measurement tissue oxygen pressure (PtiO2) and interstitial lactate and pyruvate. These measurements were carried out in anaesthetised and ventilated (FiO2 = 1) rabbits subjected to acute HS (50% of blood volume withdrawal) and either resuscitated with a PFC emulsion diluted with a 5% albumin solution (16.2 g PFC per kg body weight) (n = 10) or with a modified fluid gelatin solution (Gelofusine) (n = 10). We found no difference between the two groups for the haemodynamic and haematological variables (except for the venous oxygen partial pressure). However, a significant difference was observed in the slope of the regression linear relationship exhibited between the mean arterial pressure (MAP) and the PtiO2, PFC group showing a much steeper slope than Gelofusine group. In addition, PtiO2 values increased linearly with decreasing haematocrit (Hct) values in PFC-resuscitated animals and decreased linearly with decreasing Hct values in Gelofusine-resuscitated animals. There were no differences between the two groups concerning the blood and interstitial lactate/pyruvate ratios suggesting no deleterious effect of hyperoxic resuscitation in skeletal muscle. In conclusion these results suggest that resuscitation of severe, but brief, HS with PFC increased skeletal muscle oxygen delivery without measurable deleterious effects.
authors.affiliation: Laboratoire d'Hématologie et de Physiologie, EA 3452, Faculté de Pharmacie, Université Henri Poincaré, 5 rue Albert Lebrun, B.P. 403, F-54001 Nancy Cedex, France. Sandra.Audonnet@pharma.uhp-nancy.fr
authors.forename: Sandra
authors.initials: S
authors.lastname: Audonnet-Blaise
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Krafft
authors.forename: Younes
authors.initials: Y
authors.lastname: Smani
authors.forename: Paul-Michel
authors.initials: PM
authors.lastname: Mertes
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Marie
authors.forename: Pierre
authors.initials: P
authors.lastname: Labrude
authors.forename: Dan
authors.initials: D
authors.lastname: Longrois
authors.forename: Patrick
authors.initials: P
authors.lastname: Menu
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001802:Blood Substitutes / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005466:Fluorocarbons / Q000627:therapeutic use*; D018496:Hyperoxia / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood / Q000378:metabolism; D016014:Linear Models; D008297:Male; D008833:Microcirculation; D018482:Muscle, Skeletal / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D019289:Pyruvic Acid / Q000097:blood / Q000378:metabolism; D011817:Rabbits; D012151:Resuscitation / Q000379:methods*; D012771:Shock, Hemorrhagic / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology
chemical_list: D001802:Blood Substitutes; D005466:Fluorocarbons; D019344:Lactic Acid; D019289:Pyruvic Acid; D010100:Oxygen