[Effects of various HIV protease inhibitors on function of rat insulinoma cells].
To investigate the effects of various HIV protease inhibitors on the function of pancreatic beta-cells.



Rat insulinoma INS-1 cells were incubated with different concentrations of ritonavir or amprenavir for 48 h and stimulated with 20 mmol/L D-glucose for 30 min. The rate of insulin release was measured in the supernatant by ELISA, normalized to cellular DNA contents. Cells were counted with trypan blue and MTT test were determined to evaluate the effect of protease inhibitors on cell viability.



Ritonavir treatment significantly decreased baseline insulin release and glucose-stimulated insulin release in a dose-dependent manner (r=-0.861, -0.839, both P<0.01). For 10 micromol/L of ritonavir, the decrease rate of baseline insulin secretion and glucose-stimulated insulin secretion was 46% and 47%, respectively. Amprenavir had no effect on the rate of insulin release.



Various HIV protease inhibitors present different effect on the insulin release of pancreatic beta-cells.
Zhou
Jia-qiang
JQ
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. zhoujq@medmail.com.cn
Li
Wen-peng
WP
Xiang
Zun
Z
Schutt
Morten
M
D000818:Animals; D002219:Carbamates / Q000494:pharmacology*; D005663:Furans; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007340:Insulinoma / Q000378:metabolism* / Q000473:pathology; D007515:Islets of Langerhans / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology; D051381:Rats; D019438:Ritonavir / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*
D002219:Carbamates; D005663:Furans; D017320:HIV Protease Inhibitors; D007328:Insulin; D013449:Sulfonamides; C095108:amprenavir; D019438:Ritonavir