Proinflammatory cytokine activities, matrix metalloproteinase-3 activity, and sulfated glycosaminoglycan content in synovial fluid of dogs with naturally acquired cranial cruciate ligament rupture.
OBJECTIVE
To measure and compare activities of interleukin-1beta (IL-1beta), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and matrix metalloproteinase-3 (MMP-3); as well as sulfated glycosaminoglycan (S-GAG) content in synovial fluid from dogs with cranial cruciate ligament rupture (CCLR) and dogs with clinically normal stifles. To determine whether correlations exist between demographic and disease-related variables and these synovial markers.


METHODS
Prospective clinical study.


METHODS
Dogs with CCLR (n=23) and Beagles with normal stifle joints (n=21).


METHODS
Synovial fluid activities of proinflammatory cytokines (IL-1beta, IL-6, and TNF-alpha) were determined by bioassay. MMP-3 activity was measured using fluorogenic substrate. S-GAG contents were determined by dimethylmethylene blue dye-binding assay. Mann-Whitney U-test was used to compare results from CCLR joints with normal controls. Spearman's rank correlation test was used to evaluate associations between demographic and disease-related markers and synovial markers.


RESULTS
Mean values for synovial markers were significantly higher in CCLR joints compared with controls. IL-1beta and MMP-3 were positively correlated with lameness duration.


CONCLUSIONS
Activities of proinflammatory cytokines, MMP-3 activity and S-GAG contents were significantly elevated in synovial fluid from canine stifle joints with naturally acquired CCLR. These results indicate that there is joint inflammation and increased release of GAGs into synovial fluid, suggesting that these inflammatory changes are associated with depletion of proteoglycan from articular cartilage.


CONCLUSIONS
Medical and surgical treatments designed to decrease joint inflammation and breakdown of proteoglycans may be of value in the management of CCLR in the dog.
Fujita
Yukihiro
Y
Division of Veterinary Surgery, Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan. y-fujita@zb3.so-net.ne.jp
Hara
Yasushi
Y
Nezu
Yoshinori
Y
Schulz
Kurt S
KS
Tagawa
Masahiro
M
D000818:Animals; D000070598:Anterior Cruciate Ligament Injuries; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism; D004285:Dogs / Q000293:injuries* / Q000601:surgery; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D015601:Injury Severity Score; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007718:Knee Injuries / Q000000981:diagnostic imaging / Q000378:metabolism / Q000601:surgery / Q000662:veterinary*; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D011446:Prospective Studies; D011859:Radiography; D013582:Synovial Fluid / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015415:Biomarkers; D016207:Cytokines; D006025:Glycosaminoglycans; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D019278:Matrix Metalloproteinase 3