Role of vitamin E-coated membrane in reducing advanced glycation end products in hemodialysis patients: a pilot study.
BACKGROUND
Advanced glycation end products (AGEs) are markers of oxidative stress.


OBJECTIVE
To assess if a vitamin-E-coated dialyzer affects plasma AGE levels and endothelial function in hemodialysis patients.


METHODS
16 patients were dialyzed with a synthetic modified cellulose membrane (SMC, n = 8) or a vitamin E-coated dialyzer (n = 8), respectively. At week 32 endothelial function was determined as brachial artery flow-mediated dilatation (FMD). Total AGEs, free pentosidine (FP), protein-bound pentosidine (BP) and autoantibodies against oxidized LDL (ox-LDL-autoantibodies) were assessed at baseline (T0) and at 16, 32, 40 and 42 weeks (T16, T32, T40 and T42).


RESULTS
At T16 and T32 FP and BP were lower in vitamin E than in SMC (T 16: 88.7 +/- 8.96 vs. 124.2 +/- 11.90 pmol/ml plasma; p = 0.04, and 22.9 +/- 2.99 vs. 32.8 +/- 2.98 pmol/mg proteins; p = 0.04. T32: 78.7 +/- 8.54 vs. 123.7 +/- 10.15 pmol/ml plasma; p = 0.007, and 19.9 +/- 2.0 vs. 33.67 +/- 2.41 pmol/mg proteins; p = 0.001). In vitamin E, AGEs were lower at T32, T40 and T42 (946.7 +/- 80.91 vs. 1,351.2 +/- 179.33 AU/ml, p = 0.05; 986.9 +/- 59.63 vs. 1,509.9 +/- 154.17 AU/ml, p = 0.013; 890.3 +/- 73.70 vs. 1,453.9 +/- 153.16 AU/ml, p = 0.009). At T32 AGEs, ox-LDL autoantibodies and FMD were inversely correlated (R = -0.70 p = 0.007 and R = -0.59, p = 0.04, respectively).


CONCLUSIONS
Vit E-coated membrane reduces plasma AGEs levels and AGEs values are negatively correlated with FMD.
Baragetti
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I
Department of Medicine, Division of Nephrology and Dialysis, Cinisello Balsamo, University of Milan, Milan, Italy. i.baragetti@bassini.hsgerardo.org
Furiani
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Vettoretti
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Raselli
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S
Maggi
F M
FM
Galli
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F
Catapano
A L
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Buccianti
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D000368:Aged; D000704:Analysis of Variance; D000975:Antioxidants / Q000494:pharmacology*; D001120:Arginine / Q000031:analogs & derivatives / Q000097:blood; D001916:Brachial Artery / Q000187:drug effects / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood*; D006710:Homocysteine / Q000187:drug effects; D006801:Humans; D016014:Linear Models; D008077:Lipoproteins, LDL / Q000276:immunology; D008239:Lysine / Q000031:analogs & derivatives / Q000097:blood; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects; D010865:Pilot Projects; D006435:Renal Dialysis; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D017127:Glycation End Products, Advanced; D008077:Lipoproteins, LDL; D008567:Membranes, Artificial; D006710:Homocysteine; D014810:Vitamin E; D001120:Arginine; C062187:pentosidine; D008239:Lysine