Use of the housekeeping genes, gdh (zwf) and gki, in multilocus sequence typing to differentiate Streptococcus pneumoniae from Streptococcus mitis and Streptococcus oralis.
Polymerase chain reaction and sequencing of the housekeeping genes, gdh (zwf) and gki, based on the primers and alleles from multilocus sequence typing can be used to delineate and support the identity of clinical isolates of Streptococcus pneumoniae and differentiate from the closely related Streptococcus mitis and Streptococcus oralis.
Ip
Margaret
M
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong. margaretip@cuhk.edu.hk
Chi
Fang
F
Chau
Shirley S L
SS
Hui
Mamie
M
Tang
Julian
J
Chan
Paul K S
PK
D015373:Bacterial Typing Techniques; D005798:Genes, Bacterial; D010802:Phylogeny; D034361:Streptococcus mitis / Q000235:genetics / Q000302:isolation & purification*; D019533:Streptococcus oralis / Q000235:genetics / Q000302:isolation & purification*; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics* / Q000302:isolation & purification
D013401:Sugar Alcohol Dehydrogenases; C095946:galactitol 2-dehydrogenase