Biomolecular sensor based on fluorescence-labeled aptamer.
Fluorescent DNA probes for L-argininamide were developed by a combination of DNA aptamers and fluorophore-quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect L-argininamide specifically. The binding affinities were defined by the binding affinity of the original aptamer to indicate that the end labeling of the aptamer did not influence the affinities.
Ozaki
Hiroaki
H
Department of Chemistry, Faculty of Engineering, Gunma University, Kiryu, Japan. ozaki@chem.gunma-u.ac.jp
Nishihira
Akifumi
A
Wakabayashi
Masayuki
M
Kuwahara
Masayasu
M
Sawai
Hiroaki
H
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D052158:Aptamers, Peptide / Q000737:chemistry*; D001120:Arginine / Q000031:analogs & derivatives / Q000737:chemistry; D001483:Base Sequence; D015374:Biosensing Techniques; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004247:DNA / Q000737:chemistry; D015195:Drug Design; D005456:Fluorescent Dyes / Q000494:pharmacology; D008024:Ligands; D008956:Models, Chemical; D008969:Molecular Sequence Data; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
D052157:Aptamers, Nucleotide; D052158:Aptamers, Peptide; D005456:Fluorescent Dyes; D008024:Ligands; C069339:argininamide; D004247:DNA; D001120:Arginine