Multi-enzyme one-pot strategy for the synthesis of sialyl Lewis X-containing PSGL-1 glycopeptide.
An enzymatic one-pot three-step glycosylation strategy was developed for the synthesis of sLex moiety of truncated PSGL-1 glycopeptide with and without sulfation. The method provided an efficient way to afford complex glycopeptides in a semi-preparative scale without further complicated and time-consuming purification process in each glycosylation step.
Huang
Kuo-Ting
KT
Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei 115, Taiwan.
Wu
Bing-Ching
BC
Lin
Chang-Ching
CC
Luo
Shih-Chi
SC
Chen
Chinpan
C
Wong
Chi-Huey
CH
Lin
Chun-Cheng
CC
D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D004798:Enzymes / Q000378:metabolism*; D005647:Fucosyltransferases / Q000378:metabolism; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009237:N-Acetyllactosamine Synthase / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen; D013431:Sulfates / Q000737:chemistry / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism
D004798:Enzymes; D006020:Glycopeptides; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D013431:Sulfates; D014443:Tyrosine; D005647:Fucosyltransferases; C435083:fucosyltransferase V; D009237:N-Acetyllactosamine Synthase; D000469:Alkaline Phosphatase