title: G-CSF does not improve systolic function in a rat model of acute myocardial infarction.
abstract: OBJECTIVE
  Granulocyte colony-stimulating factor (G-CSF) has been reported to improve cardiac performance by increasing the number of bone marrow stem cell in the peripheral circulation. The aim of this study was to investigate the impact of G-CSF administration on cardiac function in a rat model of acute myocardial infarction.
  
  
  METHODS
  Recombinant human G-CSF (Filgrastim, 100 microg/kg, sc) twice a day during seven consecutive days (G-CSF group, n=13) or vehicle (control group, n=10) was administrated three hours after left anterior coronary artery ligation. Cardiac performance was evaluated 19-21 days after myocardial infarction by electro- and echocardiography, hemodynamic and treadmill exercise test.
  
  
  RESULTS
  Both infarcted groups exhibit impaired cardiac function compared to sham-operated rats. Moreover, all cardiac functional parameters were not statistically different between G-CSF and infarcted group at resting conditions as well as after treadmill exercise stress test. There was no sign of cardiac regeneration and infarct size was not different on histological analysis between groups.
  
  
  CONCLUSIONS
  These data clearly shows that G-CSF treatment was unable to prevent cardiac remodeling or to improve cardiovascular function in a rat model of acute myocardial infarction, by permanent LAD ligation, despite bone marrow stem cell mobilization.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, UFRJ CCS, Bloco G Ilha do Fundão, 21949-900 Rio de Janeiro, RJ, Brasil.
authors.forename: João Pedro S
authors.initials: JP
authors.lastname: Werneck-de-Castro
authors.forename: Ricardo Henrique
authors.initials: RH
authors.lastname: Costa-E-Sousa
authors.forename: Patricia Fidelis
authors.initials: PF
authors.lastname: de Oliveira
authors.forename: Vanessa
authors.initials: V
authors.lastname: Pinho-Ribeiro
authors.forename: Débora B
authors.initials: DB
authors.lastname: Mello
authors.forename: Ramon
authors.initials: R
authors.lastname: Peçanha
authors.forename: Elisabete
authors.initials: E
authors.lastname: Mattos
authors.forename: Emerson L
authors.initials: EL
authors.lastname: Olivares
authors.forename: Anna Carolina V
authors.initials: AC
authors.lastname: Maia
authors.forename: José Geraldo
authors.initials: JG
authors.lastname: Mill
authors.forename: Regina Coeli
authors.initials: RC
authors.lastname: Dos Santos Goldenberg
authors.forename: Antônio Carlos
authors.initials: AC
authors.lastname: Campos-de-Carvalho
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000097:blood; D004195:Disease Models, Animal; D018450:Disease Progression; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D007958:Leukocyte Count; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D010805:Physical Conditioning, Animal / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013599:Systole / Q000502:physiology*; D018487:Ventricular Dysfunction, Left / Q000503:physiopathology / Q000517:prevention & control; D020257:Ventricular Remodeling / Q000502:physiology
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor