[Research of the spermatozoal gene expression with gene microarrays].
OBJECTIVE
To perform the detection of spermatozoal gene expression in order to accelerate the study of spermatozoal molecular biology.


METHODS
To collect the healthy adults sperm and lymphocytes respectively, and then to extract the total RNAs from them by RNeasy mini kit (QIAGEN) or Trizol reagent. Corresponding cDNAs were produced, digested, ligated, finally labeled with Cy3 (sperm) and CyS (lymphocyte) in the course of RD amplifying reactions. Hybridization with self-made microarrays contained 560 probes was carried out after the labeled cDNAs pured by PCR Product Purification Kit.


RESULTS
Among the 560 probes, 72 genes were up-regulated, 321 genes were down-regulated, the others had no different expression. Furthermore, genes associated with replication, transcription, translation and regulative functions were non-different expression or down-regulated, and those belonged to the spermatogenesis associated, sperm associated antigen were up-regulated, but those involved in the glycolysis were up-regulated, in the oxidative phosphorylation were down-regulated.


CONCLUSIONS
It had successfully confirmed that there were a plenty of genes expressed in sperm, furthermore the genes expressed were accorded to spermatozoal functions and characteristics.
Mao
Xiang-Ming
XM
Department of Urology, Nanfang Hospital, Guangzhou, Guangdong 510515, China. mxm@fimmu.com
Feng
Chun-Qiong
CQ
Zou
Ya-Guang
YG
Shi
Rong
R
Song
Yan-Bin
YB
Jiang
Li
L
D000328:Adult; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification; D013094:Spermatozoa / Q000378:metabolism*; D015854:Up-Regulation
D012313:RNA