title: Simple determination of the HIV protease inhibitor atazanavir in human plasma by high-performance liquid chromatography with UV detection.
abstract: A simple high-performance liquid chromatography method for the determination of the human immunodeficiency virus protease inhibitor atazanavir in human plasma samples was developed and validated. The method involved a rapid and simple solid-phase extraction of atazanavir using Oasis HLB 1cc cartridges, an isocratic reversed-phase liquid chromatography on an XTerra RP18 (150mmx4.6mm, 3.5microm) column, and ultraviolet detection at 203nm. The mobile phase consisted of phosphate buffer (pH 6, 52.5mM) and acetonitrile (43:57, v/v). Up to 48 samples could be measured in one day since the run-time of one sample was 30min. The assay was linear from 0.04 to 10microg/ml with a lower limit of quantification of 0.04microg/ml. The mean absolute recovery of ATV was 98.1%. The method was precise, with both intra-day and inter-day coefficients of variation < or =3.0%, and accurate (deviations ranged from -3.0% to 4.5% and from -3.6% to 4.7% for intra-day and inter-day analysis, respectively). There was no interference from 35 tested potentially co-administrated drugs. This method provides a simple, sensitive, precise and reproducible assay for the therapeutic drug monitoring of atazanavir in clinical routine of laboratories with standard equipment.
authors.affiliation: Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via Gabelli 63, Padua 35121, Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Loregian
authors.forename: Silvana
authors.initials: S
authors.lastname: Pagni
authors.forename: Elisa
authors.initials: E
authors.lastname: Ballarin
authors.forename: Elisa
authors.initials: E
authors.lastname: Sinigalia
authors.forename: Saverio Giuseppe
authors.initials: SG
authors.lastname: Parisi
authors.forename: Giorgio
authors.initials: G
authors.lastname: Palù
mesh_terms: D000069446:Atazanavir Sulfate; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring; D017320:HIV Protease Inhibitors / Q000097:blood* / Q000737:chemistry; D006801:Humans; D009842:Oligopeptides / Q000097:blood* / Q000737:chemistry; D011725:Pyridines / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D017320:HIV Protease Inhibitors; D009842:Oligopeptides; D011725:Pyridines; D000069446:Atazanavir Sulfate