title: Cytotoxic ent-kaurene diterpenoids from Isodon phyllostachys.
abstract: ent-Kaurene diterpenoids, phyllostachysins D-H (1-5), together with nine known compounds, rabdoloxins A-B (6-7), rabdoinflexin B (8), amethystoidin A (9), rabdokunmin D (10), macrocalyxin E (11), 5,7-dihydroxy-4'-hydroxylflavone (12), oleanolic acid (13) and daucosterol (14), were isolated from aerial parts of Isodon phyllostachys. Structures were elucidated on the basis of spectroscopic methods, especially using 2D-NMR spectroscopic analyses. All ent-kaurenoids were tested for their cytotoxic effects against K562 cells. Compound 9 was the most potent with an IC50 value of 0.69 microg/ml.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Longquan Road, Heilongtan 610, Chinese Academy of Sciences, Kunming 650204, Yunnan, PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Weilie
authors.initials: W
authors.lastname: Xiao
authors.forename: Jianxin
authors.initials: J
authors.lastname: Pu
authors.forename: Lili
authors.initials: L
authors.lastname: Ban
authors.forename: Yunheng
authors.initials: Y
authors.lastname: Shen
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Weng
authors.forename: Shenghong
authors.initials: S
authors.lastname: Li
authors.forename: Handong
authors.initials: H
authors.lastname: Sun
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D045786:Diterpenes, Kaurane / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D006801:Humans; D020128:Inhibitory Concentration 50; D037602:Isodon / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D004224:Diterpenes; D045786:Diterpenes, Kaurane; C002627:kaurene