title: Vitreous SiO2-CaO coatings on Ti6Al4V alloys: reactivity in simulated body fluid versus osteoblast cell culture.
abstract: Vitreous coatings of the SiO(2)-CaO system have been prepared on Ti6Al4V substrates by the sol-gel method. The textural parameters (porosity and roughness) and thickness of the films obtained increase when the concentration of the precursor solutions is raised. In vitro studies of these coatings have been performed using two approaches: soaking in simulated body fluid, and by growing osteoblasts on these materials. The results of both studies show differences in terms of chemical reactivity. While in simulated body fluid the coatings were dissolved without forming a bioactive surface, when osteoblast-like cells grew on the coatings they were more stable. Furthermore, cell culture assays show biocompatible behavior of these coatings making them of potential interest for clinical applications. The effect of the textural parameters of the obtained coatings on the cell functions (attachment, spreading, proliferation and differentiation) has also been studied. The results show an increase in these cell parameters as the roughness and porosity of the coatings increase.
authors.affiliation: Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Izquierdo-Barba
authors.forename: F
authors.initials: F
authors.lastname: Conde
authors.forename: N
authors.initials: N
authors.lastname: Olmo
authors.forename: M A
authors.initials: MA
authors.lastname: Lizarbe
authors.forename: M A
authors.initials: MA
authors.lastname: García
authors.forename: M
authors.initials: M
authors.lastname: Vallet-Regí
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D000497:Alloys; D015415:Biomarkers / Q000378:metabolism; D001826:Body Fluids / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D003198:Computer Simulation; D005898:Glass / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000497:Alloys; D015415:Biomarkers; D017610:Calcium Compounds; D020099:Coated Materials, Biocompatible; D010087:Oxides; C031462:titanium alloy (TiAl6V4); C016538:lime; D014025:Titanium; D000469:Alkaline Phosphatase