[Genetic diversity analysis of isolates belonging to Bordetella genus].
In this study, RandomAmplified Polymorphic DNA (RAPD) method was used to track differences among human and animals isolates of eight known species of Bordetella genus. One hundred representative strains of these species from international and Polish bacterial collections were genotyped according to RAPD protocols using primer 1254 or 1247. Problems with reproducibility and discriminatory power, frequently discussed in literature, have been overcome by precise optimization procedure, which allowed to achieve reliable conditions for Bordetella species analysed. This study proved high potential of RAPD method using primers 1247/1254 for intra-species differentiation of B. bronchiseptica and B. hinzii isolates. Additionally RAPD method has been found to be useful for confirming B. avium and B. holmesii identification.
Mosiej
Ewa
E
Zakłład Badania Surowic i Szczepionek PZH w Warszawie.
Gzyl
Anna
A
Slusarczyk
Janusz
J
D000818:Animals; D001884:Bordetella / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D017931:DNA Primers