Visualization of human sperm chromosomes by using mouse oocytes cryothawed after enucleation.
OBJECTIVE
To evaluate whether previously enucleated mouse oocytes that are vitrified are usable for the analysis of human sperm chromosomes.


METHODS
Prospective, comparative laboratory study.


METHODS
Animal breeding and reproduction department in a rural Japan university.


METHODS
Sperm samples were obtained from a fertile donor.


METHODS
Human sperm were injected into previously enucleated-vitrified mouse oocytes.


METHODS
The rates of appearance of the human sperm chromosomes that were injected into previously enucleated-vitrified mouse oocytes.


RESULTS
The rates of appearance of human sperm chromosomes were 71%-72% in enucleated-vitrified mouse oocytes.


CONCLUSIONS
The use of enucleated-vitrified mouse oocytes allowed visualization of human sperm chromosomes.
Araki
Yasuyuki
Y
Science of Plant and Animal Production, United Graduated School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan. yaaraki@nifty.com
Yoshizawa
Midori
M
Araki
Yasuhisa
Y
D000818:Animals; D002467:Cell Nucleus; D002470:Cell Survival; D002478:Cells, Cultured; D002877:Chromosomes, Human; D015925:Cryopreservation; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D009865:Oocytes / Q000502:physiology; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000502:physiology*; D013396:Suction