title: Phi-analysis of the folding of the B domain of protein A using multiple optical probes.
abstract: We examined the co-operativity of ultra-fast folding of a protein and whether the Phi-value analysis of its transition state depended on the location of the optical probe. We incorporated in turn a tryptophan residue into each of the three helices of the B domain of Protein A. Each Trp mutant of the three-helix bundle protein was used as a pseudo-wild-type parent for Phi-analysis in which the intrinsic Trp fluorescence probed the formation of each helix during the transition state. Apart from local effects in the immediate vicinity of the probe, the three separate sets of Phi-values were in excellent agreement, demonstrating the overall co-operativity of folding and the robustness of the Phi-analysis. The transition state of folding of Protein A contains the second helix being well formed with many stabilizing tertiary hydrophobic interactions. In contrast, the first and the third helices are more poorly structured in the transition state. The mechanism of folding thus involves the concurrent formation of secondary and tertiary interactions, and is towards the nucleation-condensation extreme in the nucleation-condensation-framework continuum of mechanism, with helix 2 being the nucleus. We provide an error analysis of Phi-values derived purely from the kinetics of two-state chevron plots.
authors.affiliation: MRC Centre for Protein Engineering, Hills Road, Cambridge CB2 2QH, UK.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sato
authors.forename: Tomasz L
authors.initials: TL
authors.lastname: Religa
authors.forename: Alan R
authors.initials: AR
authors.lastname: Fersht
mesh_terms: D002942:Circular Dichroism; D019791:Guanidine / Q000737:chemistry; D006860:Hydrogen Bonding; D007700:Kinetics; D009154:Mutation / Q000235:genetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D015203:Reproducibility of Results; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000737:chemistry*; D013816:Thermodynamics; D014364:Tryptophan / Q000235:genetics
chemical_list: D013205:Staphylococcal Protein A; D014364:Tryptophan; D019791:Guanidine