title: Runx3 expression during mouse tongue and palate development.
abstract: Studies of molecular mechanisms underlying the development of the mammalian oral mucosa have revealed a major involvement of transforming growth factor beta (TGF-beta) and bone morphologic protein (BMP) signaling pathways. Here, we examined the expression of a downstream target of TGF-beta and BMPs, Runx3, in oral mucosa. Runx3 is a runt-related transcription factor that acts as a gastric tumor suppressor and regulator of growth and differentiation in mammalian gastric epithelial cells. Another member of the Runx family in C. elegans, run, is involved in the development of a functional hypodermis and gut. In this report, we examined Runx3 expression using reverse transcription-polymerase chain reaction, immnunohistochemistry, and in situ hybridization and found that Runx3 is expressed in the tongue and palate epithelium of mouse embryos from embryonic day 12.5 to 16.5. The functional relationship between Runx3 and TGF-beta/BMPs signaling in tongue and palate development is discussed.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan. hyamamot@kuhp.kyoto-u.ac.jp
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Kosei
authors.initials: K
authors.lastname: Ito
authors.forename: Mariko
authors.initials: M
authors.lastname: Kawai
authors.forename: Yota
authors.initials: Y
authors.lastname: Murakami
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Bessho
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D050677:Core Binding Factor Alpha 3 Subunit / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D010159:Palate / Q000254:growth & development* / Q000378:metabolism*; D014059:Tongue / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; C493730:Runx3 protein, mouse