Roles of the Nanog protein in murine F9 embryonal carcinoma cells and their endoderm-differentiated counterparts.
Nanog is a recently discovered homeodomain transcription factor that sustains the pluripotency of embryonic stem (ES) cells and blocks their differentiation into endoderm. The murine F9 embryonal carcinoma cell line is a well-documented model system for endoderm cell lineage differentiation. Here, we examined the function of Nanog in F9 cell endoderm differentiation. Over-expression of Nanog returns the F9 cells to the early status of ES cells and represses the differentiation of primitive endoderm and parietal endoderm in F9 cells, whereas it has no effect on the differentiation of visceral endoderm. In contrast, the expression of C-terminal domain-truncated Nanog spontaneously promotes endoderm differentiation in F9 cells. These data suggest that Nanog is required to sustain the proper undifferentiated status of F9 cells, and the C-terminal domain of Nanog transduces the most effects in repressing primitive endoderm and parietal endoderm differentiation in F9 cells.
Chen
Yanmei
Y
Laboratory of Molecular and Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China. chenym@sibs.ac.cn
Du
Zhongwei
Z
Yao
Zhen
Z
D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D048430:Cell Shape; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism*; D054278:Embryonal Carcinoma Stem Cells; D004707:Endoderm / Q000166:cytology* / Q000502:physiology; D018398:Homeodomain Proteins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D000071317:Nanog Homeobox Protein; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000502:physiology*; D014212:Tretinoin / Q000378:metabolism
D000970:Antineoplastic Agents; D015415:Biomarkers; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D014212:Tretinoin