title: Multiple initial culture conditions enhance the establishment of cell lines from primary ovarian cancer specimens.
abstract: To increase the efficiency of stable cell line establishment from primary ovarian cancer specimens, we simultaneously initiated cultures under multiple conditions, varying extracellular matrices and the inclusion of supplements (e.g., serum or serum albumin), while minimizing exposure to xenogeneic antigens (e.g., fetal calf serum). Primary cultures were initiated from 30 specimens; cell lines were established from 10 of these for a success rate of 33%. In some instances, multiple cell lines were established from the same specimen. Five lines were characterized extensively with respect to growth properties, antigen expression, and genomic alterations. Although these lines are all low-passage, marked heterogeneity was observed, even between lines derived from the same specimen. The culture approach outlined herein will facilitate generation of reagents useful for many aspects of ovarian cancer biology.
authors.affiliation: Abramson Family Cancer Research Institute, Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19104-6160, USA.
authors.forename: Cara C
authors.initials: CC
authors.lastname: Bertozzi
authors.forename: Cheng-Yi
authors.initials: CY
authors.lastname: Chang
authors.forename: Sonya
authors.initials: S
authors.lastname: Jairaj
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Shan
authors.forename: Jia
authors.initials: J
authors.lastname: Huang
authors.forename: Barbara L
authors.initials: BL
authors.lastname: Weber
authors.forename: Christina S
authors.initials: CS
authors.lastname: Chu
authors.forename: Richard G
authors.initials: RG
authors.lastname: Carroll
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured