Prevalence of extended-spectrum beta-lactamases (ESBLs) produced in blood isolates of gram-negative bacteria in a teaching hospital in southern Thailand.
One hundred and eighty-three strains of gram-negative bacteria were isolated from 177 bacteremic patients during a 6-month period in 2004 at a teaching hospital in southern Thailand. Extended-spectrum beta-lactamases (ESBL) production was detected in K. pneumoniae, E. coli, and E. cloacae, at rates of 16/36 (44.4%), 3/59 (5.1%), and 2/13 (15.4%), respectively. All but one of the screened positive strains were also positive by both the combination disk method and the E-test. All of the K. pneumoniae strains that were resistant to ceftazidime by disk diffusion were demonstrated to be positive for ESBL production by both the combination disk and E-test methods. Most of the ESBL positive strains had a high MIC (more than 32 microg/ml) to ceftazidime. However, all the ESBL positive strains were sensitive to imipenem.
Jitsurong
Siroj
S
Department of Pathology, Faculty of Medicine, Prince of Songkla University, Thailand. sirojj@yahoo.com
Yodsawat
Jareerat
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000453:epidemiology / Q000382:microbiology*; D015169:Colony Count, Microbial; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification; D006784:Hospitals, Teaching; D006801:Humans; D008826:Microbial Sensitivity Tests; D013785:Thailand / Q000453:epidemiology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001618:beta-Lactamases