Production of vanillin from waste residue of rice bran oil by Aspergillus niger and Pycnoporus cinnabarinus.
A new technology of transforming ferulic acid, which was from waste residue of rice bran oil, into vanillin was developed by a combination of fungal strains Aspergillus niger CGMCC0774 and Pycnoporus cinnabarinus CGMCC1115. Various concentrations of ferulic acid were compared, and the highest yield reached 2.2 g l(-1) of vanillic acid by A. niger CGMCC0774 in a 25 l fermenter when concentration of ferulic acid was 4 g l(-1). The filtrate of A. niger CGMCC0774 culture was concentrated and vanillic acid in the filtrate was bio-converted into vanillin by P. cinnabarinus CGMCC1115. The yield of vanillin reached 2.8 g l(-1) when 5 g l(-1) of glucose and 25 g of HZ802 resin were supplemented in the bioconversion medium. The 13C isotope analysis indicated that delta13C(PDB) of vanillin prepared was much different from chemically synthesized vanillin.
Zheng
Lirong
L
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi, 214036 Jiangsu, China.
Zheng
Pu
P
Sun
Zhihao
Z
Bai
Yanbing
Y
Wang
Jun
J
Guo
Xinfu
X
D001234:Aspergillus niger / Q000378:metabolism*; D001547:Benzaldehydes / Q000737:chemistry / Q000378:metabolism*; D019149:Bioreactors; D001711:Biotransformation / Q000187:drug effects; D002250:Carbon Radioisotopes / Q000032:analysis; D003247:Conservation of Natural Resources; D003373:Coumaric Acids / Q000378:metabolism / Q000494:pharmacology; D010938:Plant Oils / Q000378:metabolism*; D011124:Polyporaceae / Q000378:metabolism*; D000073879:Rice Bran Oil; D014641:Vanillic Acid / Q000378:metabolism / Q000494:pharmacology; D014865:Waste Disposal, Fluid / Q000379:methods*
D001547:Benzaldehydes; D002250:Carbon Radioisotopes; D003373:Coumaric Acids; D010938:Plant Oils; C004999:ferulic acid; C100058:vanillin; D014641:Vanillic Acid; D000073879:Rice Bran Oil