title: High rate of genetic recombination in murine leukemia virus: implications for influencing proviral ploidy.
abstract: A significant difference in the recombination rates between human immunodeficiency virus type 1 (HIV-1) and the gammaretroviruses was previously reported, with the former being 10 to 100 times more recombinogenic. It is possible that preferential copackaging of homodimers in the case of gammaretroviruses, like murine leukemia virus (MLV), led to the underestimation of their rates of recombination. To reexamine the recombination rates for MLV, experiments were performed to control for nonrandom copackaging of viral RNA, and it was found that MLV and HIV-1 exhibit similar crossover rates. The implications for control of proviral ploidy and preferential recombination during minus-strand DNA synthesis are discussed.
authors.affiliation: Department of Molecular Genetics, Microbiology and Immunology, UMDNJ/Robert W. Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854-5635, USA.
authors.forename: Jianling
authors.initials: J
authors.lastname: Zhuang
authors.forename: Sayandip
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Yacov
authors.initials: Y
authors.lastname: Ron
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Dougherty
mesh_terms: D000818:Animals; D003434:Crossing Over, Genetic / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics*; D041681:NIH 3T3 Cells; D011003:Ploidies; D012367:RNA, Viral / Q000235:genetics*; D019065:Virus Assembly / Q000235:genetics*
chemical_list: D012367:RNA, Viral