Human endothelial cell septins: SEPT11 is an interaction partner of SEPT5.
The septin SEPT11 is a novel member of the highly conserved septin family. Septins are cytoskeletal GTPases, which form heteropolymeric complexes. They are involved in cytokinesis and other cellular processes, such as vesicle trafficking and exocytosis. SEPT11 has strong homology to SEPT8. Previously, we identified the interaction of SEPT5 and SEPT8. Using the yeast two-hybrid system, we now demonstrate that SEPT11 partners with SEPT5. The molecular interaction of SEPT11 with SEPT5 was verified by coprecipitation of SEPT5 and SEPT11 from lysates of the human T-cell leukaemia cell line JURKAT and by fluorescence resonance energy transfer. The interaction between SEPT5 and SEPT11 requires the GTP-binding domain and the C-terminal extension. Western analysis in various mouse and human tissues revealed that expression of SEPT11 is restricted to the same tissues as those expressing SEPT5, suggesting that SEPT11 and SEPT5 are components of a cell-specific septin complex. SEPT5, which is expressed in human umbilical vein endothelial cells (HUVECs), has been reported to play an important role in exocytosis. We now report that HUVECs also express SEPT11. Given the interactivity between SEPT5 and SEPT11 as shown above and their coexpression in HUVECs, it may be that a complex formed by these two proteins is involved in the exocytosis mechanism in HUVECs.
Bläser
S
S
Department of Paediatrics and Adolescent Medicine, University Hospital Freiburg, Mathildenstrasse 1, D-79106 Germany.
Röseler
S
S
Rempp
H
H
Bartsch
I
I
Bauer
H
H
Lieber
M
M
Lessmann
E
E
Weingarten
L
L
Busse
A
A
Huber
M
M
Zieger
B
B
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001923:Brain Chemistry / Q000235:genetics; D019556:COS Cells; D018797:Cell Cycle Proteins / Q000502:physiology*; D011232:Chemical Precipitation; D002522:Chlorocebus aethiops; D004347:Drug Interactions; D042783:Endothelial Cells / Q000502:physiology*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005786:Gene Expression Regulation / Q000235:genetics; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D018482:Muscle, Skeletal / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D009363:Neoplasm Proteins / Q000502:physiology*; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism; D058112:Septins; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000378:metabolism
D018797:Cell Cycle Proteins; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D006160:Guanosine Triphosphate; D001616:beta-Galactosidase; C496549:SEPTIN11 protein, human; C109366:SEPTIN5 protein, human; D058112:Septins