Selective upregulation of beta1-adrenergic receptors and dephosphorylation of troponin I in end-stage heart failure patients supported by ventricular assist devices.
In terminal failing hearts, adrenergic receptors are downregulated and intracellular adrenergic signal transduction is inhibited. Mechanical circulatory support by ventricular assist devices (VAD) is used to bridge patients to heart transplantation. Mechanical unloading by VAD may induce reverse remodeling in heart transplantation (HTx) candidates. However, little is known on beta-adrenergic receptor subtype regulation and adrenergic signal transduction under VAD-support. We investigated paired myocardial samples from 16 VAD-supported patients and 9 non-failing donor hearts. We analyzed beta-adrenergic receptor subtype regulation by real-time PCR and radioligand binding and cardiac troponin I phosphorylation (by phospho-cTnI-specific antibodies). We found that the beta1-adrenergic receptor (beta1AR) is downregulated at VAD-implantation on mRNA and protein levels whereas the beta2-adrenergic receptor (beta2AR) was not. After VAD-support, beta1AR protein but not its mRNA was upregulated, whereas the degree of cTnI-phosphorylation was reduced. Upregulation of beta1AR was enhanced by beta blocking medication during VAD-support. However, in 9 out of 15 patients, beta1AR-density remained below the 0.25 percentile of donor hearts. VAD-support is associated with partial normalization of the betaAR-signal transduction pathways. This beneficial effect is related to a posttranscriptional increase in beta1AR-density.
Milting
Hendrik
H
Ruhr-Universität Bochum; Herz- and Diabeteszentrum NRW, Erich and Hanna Klessmann-Institut für Kardiovaskuläre Forschung and Entwicklung; 32545 Bad Oeynhausen, Germany. hmilting@hdz-nrw.de
Scholz
Christian
C
Arusoglu
Latif
L
Freitag
Meike
M
Cebulla
Ramona
R
Jaquet
Kornelia
K
Körfer
Reiner
R
V Lewinski
Dirk
D
Kassner
Astrid
A
Brodde
Otto-Erich
OE
Kögler
Harald
H
El Banayosy
Aly
A
Pieske
Burkert
B
D000328:Adult; D000368:Aged; D005260:Female; D005786:Gene Expression Regulation; D006331:Heart Diseases / Q000378:metabolism*; D006333:Heart Failure / Q000378:metabolism*; D006352:Heart Ventricles / Q000473:pathology*; D006353:Heart-Assist Devices; D006801:Humans; D008297:Male; D008875:Middle Aged; D010766:Phosphorylation; D018342:Receptors, Adrenergic, beta-1 / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D019210:Troponin I / Q000378:metabolism*; D015854:Up-Regulation
D018342:Receptors, Adrenergic, beta-1; D019210:Troponin I