CRP determination based on a novel magnetic biosensor.
The c-reactive protein (CRP) is a very significant human blood marker for inflammatory processes and is routinely determined for many clinical purposes. The widespread and well established detection method for this approximately 115 kDa hepatic protein is the high-sensitivity ELISA assay (hsCRP-ELISA) in blood serum. New approaches in medical CRP diagnosis (e.g. for CVD, inflammatory bowel disease) require rapid quantification in native matrices. A novel CRP determination method based on magnetic detection is described and tested for human blood serum, saliva and urine. The detection principle is based on two different anti-CRP antibodies (monoclonal, IgG) for CRP trapment and labelling. The linear detection range of this immunosensor ranged from 25 ng/ml to 2.5 microg/ml and is therefore much more sensitive than typical hsCRP-ELISA-assays.
Meyer
Martin H F
MH
Institute for Pharmaceutical Chemistry, Philipps-University, Marburg, Germany. Martin.Meyer@staff.uni-marburg.de
Hartmann
Markus
M
Krause
Hans-Joachim
HJ
Blankenstein
Gert
G
Mueller-Chorus
Birgit
B
Oster
Juergen
J
Miethe
Peter
P
Keusgen
Michael
M
D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D002097:C-Reactive Protein / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D018189:Immunomagnetic Separation / Q000295:instrumentation* / Q000379:methods; D007249:Inflammation / Q000097:blood / Q000175:diagnosis; D008280:Magnetics / Q000295:instrumentation*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity
D015415:Biomarkers; D002097:C-Reactive Protein