Tissue-specific regulation of acetyl-CoA carboxylase gene expression by dietary soya protein isolate in rats.
We have recently reported that intake of soya protein isolate (SPI) inhibited the DNA-binding activities of hepatic thyroid hormone receptor (TR). The genes for acetyl-CoA carboxylase (ACC), a rate-limiting enzyme in fatty acid synthesis, contain the thyroid hormone response element in their promoters and are regulated by TR. The present study has examined the effect of long-term feeding of SPI and soya isoflavones (ISF) on the gene expression and protein phosphorylation of different ACC isoforms in different tissues and plasma triacylglycerol (TAG) levels in rats. Sprague-Dawley female rats were fed diets containing 20 % casein or alcohol-washed SPI with or without supplemental ISF for 70, 190 and 310 d. SPI intake significantly reduced plasma TAG concentrations compared with casein, whereas supplemental ISF had no effect. Hepatic ACCalpha and ACCbeta mRNA abundance and protein content were markedly lower in the rats fed SPI than in those fed casein. The protein contents of ACCalpha in the kidney and ACCbeta, the predominant isoform in the heart and kidney, were unchanged by dietary SPI. The ratios of phospho-ACCalpha/ACCalpha and phospho-ACCbeta/ACCbeta were not different among dietary groups in all tissues measured. The present study demonstrates that ingestion of SPI decreases plasma TAG level and down-regulates ACCalpha and ACCbeta gene expression in the liver but not in the heart and kidney. The results indicate that the effect of SPI is tissue-specific and that alteration of ACC gene expression rather than phosphorylation status may play a major role in the regulation of ACC activities by soya proteins.
Xiao
Chao Wu
CW
Nutrition Research Division, Food Directorate, Health Products and Food Branch, Health Canada, 2203C Banting Research Centre, Ottawa, ON, Canada K1A 0L2. chaowu_xiao@hc-sc.gc.ca
Wood
Carla
C
Huang
Wenxin
W
L'Abbé
Mary R
MR
Gilani
G Sarwar
GS
Cooke
Gerard M
GM
Curran
Ivan
I
D000103:Acetyl-CoA Carboxylase / Q000032:analysis / Q000235:genetics*; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007328:Insulin / Q000097:blood; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics; D020738:Leptin / Q000097:blood; D008099:Liver / Q000201:enzymology*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D030262:Soybean Proteins / Q000494:pharmacology*; D014280:Triglycerides / Q000097:blood
D007328:Insulin; D007527:Isoenzymes; D020738:Leptin; D012333:RNA, Messenger; D030262:Soybean Proteins; D014280:Triglycerides; D000103:Acetyl-CoA Carboxylase