title: Polysaccharide microarray technology for the detection of Burkholderia pseudomallei and Burkholderia mallei antibodies.
abstract: A polysaccharide microarray platform was prepared by immobilizing Burkholderia pseudomallei and Burkholderia mallei polysaccharides. This polysaccharide array was tested with success for detecting B. pseudomallei and B. mallei serum (human and animal) antibodies. The advantages of this microarray technology over the current serodiagnosis of the above bacterial infections were discussed.
authors.affiliation: Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA. narayanan.parthasarathy@amedd.army.mil
authors.forename: Narayanan
authors.initials: N
authors.lastname: Parthasarathy
authors.forename: David
authors.initials: D
authors.lastname: DeShazer
authors.forename: Marilyn
authors.initials: M
authors.lastname: England
authors.forename: David M
authors.initials: DM
authors.lastname: Waag
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D042726:Burkholderia mallei / Q000276:immunology / Q000302:isolation & purification*; D016957:Burkholderia pseudomallei / Q000276:immunology / Q000302:isolation & purification*; D005896:Glanders / Q000175:diagnosis / Q000382:microbiology; D008554:Melioidosis / Q000175:diagnosis / Q000382:microbiology; D046228:Microarray Analysis / Q000379:methods*; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial