title: Analyses of RAS regulation of eye development in Drosophila melanogaster.
abstract: Many aspects of Drosophila eye development depend on receptor tyrosine kinases that signal through Ras. Genetic studies and genetic screens using eye morphology and development as assays have identified major components of receptor tyrosine kinase and Ras signaling and outlined specific contributions of these components to cell fate specification and differentiation, cell survival, cell cycle progression and arrest, and cellular movements and morphology. This chapter presents a brief compendium of methods and strains that may be used to obtain overexpression or loss of function for Ras pathway genes in the eye and methods and reagents permitting initial characterization of retinal cell differentiation, death, and cell cycle behavior.
authors.affiliation: Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Lucy C
authors.initials: LC
authors.lastname: Firth
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Baker
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D016923:Cell Death; D002454:Cell Differentiation; D049109:Cell Proliferation; D004254:DNA Nucleotidyltransferases / Q000502:physiology; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development*; D005123:Eye / Q000254:growth & development*; D019076:Transgenes / Q000502:physiology; D018631:ras Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; D018631:ras Proteins