[Valsartan eluting-stents inhibited neointimal hyperplasia by decreasing collagen deposition in rabbits].
OBJECTIVE
To assess the effect of valsartan eluting-stents on restenosis and collagen deposition in neointima hyperplasia in rabbits.


METHODS
Valsartan eluting-stents and the carrier eluting-stents were made with patented multi-layers coating techniques. Bare stents (n = 8), carrier eluting-stents (n = 8) and valsartan eluting-stents (n = 10) were implanted into rabbit abdominal aortas, respectively. Quantitive angiography (QA) was performed before, immediately post and 3 months after stents implantations to determine the diameter of aortas. Rabbits were killed 3 months post stents implantation and the cross sections of the stented vessels were analyzed for neointimal formation: luminal area (LA), neointimal area (NIA), inner elastic lumina area (IELA), the maximal inner-membrane thickness (MIT) and percent stenosis. MASSON and picrosirius red staining were performed to observe the collagen deposition in neointima analyzed.


RESULTS
The mean aortic diameters measured by QA at different time points were similar between the groups. LA was significantly larger (5 016 269 microm(2) +/- 207,934 microm(2) vs. 4,345,548 microm(2) +/- 125,822 microm(2) and 4,302,061 microm(2) +/- 167,952 microm(2), P < 0.01 vs. valsartan stents) while NIA (441,577 microm(2) +/- 74,099 microm(2) vs. 1,119,635 microm(2) +/- 163,503 microm(2) and 1,135,636 microm(2) +/- 136,555 microm(2)) and MIT (116 microm +/- 12 microm vs. 240 microm +/- 30 microm and 192 microm +/- 21 microm) as well as percent stenosis (8% +/- 2% vs. 20% +/- 2% and 21% +/- 2%) were significantly reduced in valsartan eluting-stents group compared to bare and carrier stents groups. MASSON and picrosirius red staining revealed rich type III collagen deposition in neointima and spare type I collagen patched around stents struts in bare and carrier stents groups and collagen deposition was rarely seen in neointima and stents struts in valsartan eluting-stents group.


CONCLUSIONS
Valsartan eluting-stents inhibited neointimal hyperplasia by decreasing collagen deposition.
Wang
Lei
L
Cardiovascular Center, Beijing Friendship Hospital Affiliated of Capital University of Medicine Sciences, Beijing 100050, China.
Jia
San-qing
SQ
Li
Gui-hua
GH
Chen
Hui
H
Li
Hong-wei
HW
Zhao
Lin
L
Yao
Dao-kuo
DK
Ding
Rong-jing
RJ
D000818:Animals; D003094:Collagen / Q000378:metabolism*; D023903:Coronary Restenosis / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D003331:Coronary Vessels / Q000473:pathology; D054855:Drug-Eluting Stents; D005260:Female; D006083:Graft Occlusion, Vascular / Q000378:metabolism / Q000473:pathology*; D006965:Hyperplasia; D008297:Male; D011817:Rabbits; D013777:Tetrazoles / Q000627:therapeutic use*; D017539:Tunica Intima / Q000473:pathology; D014633:Valine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D000068756:Valsartan
D013777:Tetrazoles; D000068756:Valsartan; D003094:Collagen; D014633:Valine