Crystallization and preliminary X-ray analysis of the Rieske-type [2Fe-2S] ferredoxin component of biphenyl dioxygenase from Pseudomonas sp. strain KKS102.
BphA3, a Rieske-type [2Fe-2S] ferredoxin component of a biphenyl dioxygenase (BphA) from Pseudomonas sp. strain KKS102, was crystallized by the hanging-drop vapour-diffusion method. Two crystal forms were obtained from the same conditions. The form I crystal belongs to space group P2(1)2(1)2, with unit-cell parameters a = 26.3, b = 144.3, c = 61.5 A, and diffracted to 2.45 A resolution. The form II crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 26.2, b = 121.3, c = 142.7 A, and diffracted to 2.8 A resolution. A molecular-replacement calculation using BphF as a search model yielded a satisfactory solution for both forms.
Senda
Miki
M
Japan Biological Information Research Center (JBIRC), Japan Biological Informatics Consortium (JBIC), 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan.
Kimura
Shigenobu
S
Kishigami
Shinya
S
Senda
Toshiya
T
D001426:Bacterial Proteins / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D049308:Dioxygenases / Q000737:chemistry*; D005288:Ferredoxins / Q000737:chemistry*; D007506:Iron-Sulfur Proteins / Q000737:chemistry; D010105:Oxygenases / Q000737:chemistry; D011549:Pseudomonas / Q000201:enzymology*; D012997:Solvents; D014961:X-Ray Diffraction
C081714:2Fe-2S ferredoxin; D001426:Bacterial Proteins; D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D012997:Solvents; D010105:Oxygenases; D049308:Dioxygenases; C074161:biphenyl-2,3-dioxygenase