Differential in vivo effects of whole cigarette smoke exposure versus cigarette smoke extract on mouse ciliated tracheal epithelium.
In this study the authors compared the affect of vapor phase cigarette smoke (CS) versus cigarette smoke extract (CSE) on the lungs and upper airway of C57BL/6 mice. The authors found that CSE treatment significantly increased neutrophil influx (P < .001), baseline ciliary beat frequency (CBF) (P < .05), and protein kinase C activity compared to CS and controls. Isoproterenol increased CBF with CS exposure, but decreased CBF with CSE (P < .01). Isoproterenol increased protein kinase A (PKA) activity in all groups except CSE. CSE exposure induced inflammatory cell bronchiolitis. These data indicate that CSE exposure has differential effects on the lungs and tracheal epithelium compared to CS exposure.
Elliott
Margaret K
MK
Pulmonary, Critical Care and Sleep Medicine Section, University of Nebraska Medical Center, Omaha, Nebraska 68198-5300, USA.
Sisson
Joseph H
JH
West
William W
WW
Wyatt
Todd A
TA
D000280:Administration, Inhalation; D000281:Administration, Intranasal; D000818:Animals; D001835:Body Weight; D001988:Bronchiolitis / Q000209:etiology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002923:Cilia / Q000187:drug effects*; D003367:Cotinine / Q000097:blood; D017868:Cyclic AMP-Dependent Protein Kinases / Q000187:drug effects*; D007545:Isoproterenol; D051379:Mice; D008810:Mice, Inbred C57BL; D020545:Respiratory Mucosa / Q000187:drug effects*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014132:Trachea / Q000166:cytology / Q000187:drug effects
D014028:Tobacco Smoke Pollution; D017868:Cyclic AMP-Dependent Protein Kinases; D003367:Cotinine; D007545:Isoproterenol