title: Antioxidant and anti-inflammatory activity of extract obtained from Aspergillus candidus MTCC 2202 broth filtrate.
abstract: Antioxidant potential of Aspergillus candidus MTCC 2202 broth filtrate extract was studied using different antioxidant models, whereas anti-inflammatory potential was studied using carrageenan-induced rat paw oedema model. The ethyl acetate extract at 1000 microg/ml showed maximum scavenging activity of the stable radical 1,1-diphenyl,2-picryl hydrazyl upto 96.65% (IC50=430.36 microg/ml) and scavenging of the radical cation, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) upto 92.25% (IC50=606.29 microg/ml) at the same concentration. The extract had good reducing power, however showed moderate inhibition for conjugated dienes and thiobarbituric acid reactive acid substances (59.56 and 51.45%). The total phenolic content of various extracts of A. candidus broth filtrate was measured and a correlation between radical scavenging activities of extracts with total phenolic content was observed. The ethyl acetate extract (125 mg/kg ip) showed significant anti-inflammatory activity in carrageenan-induced rat paw oedema model. The exhibited antioxidant activity of ethyl acetate extract of A. candidus broth filtrate was comparable with BHA and ascorbic acid, while anti-inflammatory activity was comparable with standard diclofenac sodium.
authors.affiliation: Department of Pharmaceutical Sciences & Technology, Institute of Chemical Technology, University of Mumbai. Matunga, Mumbai 400 019, India.
authors.forename: Pankaj P
authors.initials: PP
authors.lastname: Malpure
authors.forename: Abhishek S
authors.initials: AS
authors.lastname: Shah
authors.forename: Archana R
authors.initials: AR
authors.lastname: Juvekar
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001230:Aspergillus / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000737:chemistry; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002412:Cations; D003470:Culture Media / Q000378:metabolism; D004008:Diclofenac / Q000494:pharmacology; D004487:Edema / Q000188:drug therapy; D005609:Free Radicals; D006834:Hydrazines / Q000737:chemistry; D019800:Phenol; D010851:Picrates; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D002412:Cations; D003470:Culture Media; D005609:Free Radicals; D006834:Hydrazines; D010851:Picrates; D004008:Diclofenac; D002083:Butylated Hydroxyanisole; D019800:Phenol; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid