[Transgenic wheat (Triticum aestivum L.) with increased resistance to the storage pest obtained by Agrobacterium tumefaciens--mediated].
The transgenic wheat of improved resistance to the storage pest was production. We have introduced the cowpea trypsin inhibitor gene (CpTI) into cultured embryonic callus cells of immature embryos of wheat elite line by Agrobacterium-mediated method. Independent plantlets were obtained from the kanamycin-resistant calli after screening. PCR and real time PCR analysis, PCR-Southern and Southern blot hybridization indicated that there were 3 transgenic plants viz. transformed- I, II and III (T- I, T-II and T-III). The transformation frequencies were obviously affected by Agrobacterium concentration, the infection duration and transformation treatment. The segregations of CpTI in the transgenic wheat progenies were not easily to be elucidated, and some transgenic wheat lines (T- I and T-III) showed Mendelian segregations. The determinations of insect resistance to the stored grain insect of wheat viz. the grain moth (Sitotroga cerealella Olivier) indicated that the 3 transgenic wheat progeny seeds moth-resistance was improved significantly. The seed moth-eaten ratio of T- I, T-II, T-III and nontransformed control was 19.8%, 21.9%, 32.9% and 58.3% respectively. 3 transgenic wheat T1 PCR-positive plants revealed that the 3 transgenic lines had excellent agronomic traits. They supplied good germplasm resource of insect-resistance for wheat genetic improvement.
Bi
Rui-Ming
RM
Subcenter of National Wheat Improvement Center, The Key Laboratory of Crop Biology of Shandong, Agronomy College of Shandong Agricultural University, Tainan 271018, China.
Jia
Hai-Yan
HY
Feng
De-Shun
DS
Wang
Hong-Gang
HG
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000818:Animals; D006927:Hymenoptera / Q000254:growth & development; D007915:Lepidoptera / Q000254:growth & development; D018532:Pisum sativum / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics*; D014361:Trypsin Inhibitors / Q000096:biosynthesis / Q000235:genetics*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D010940:Plant Proteins; D014361:Trypsin Inhibitors; D000516:alpha-Amylases