Fumonisin concentration and ceramide synthase inhibitory activity of corn, masa, and tortilla chips.
Nixtamalization removes fumonisins from corn and reduces their amounts in masa and tortilla products. Fumonisin concentrations and potential toxicity could be underestimated, however, if unknown but biologically active fumonisins are present. Therefore, the relative amounts of fumonisins in extracts of fumonisin-contaminated corn and its masa and tortilla chip nixtamalization products were determined with an in vitro ceramide synthase inhibition bioassay using increased sphinganine (Sa) and sphinganine to sphingosine ratio (Sa/So) as endpoints. African green monkey kidney cells (Vero cells ATCC CCL-81) were grown in 1-ml wells and exposed to 4 microl of the concentrated extracts for 48 h. The corn extract inhibited ceramide synthase as Sa (mean = 132 pmol/well) and Sa/So (mean = 2.24) were high compared to vehicle controls (Sa = 9 pmol/well; Sa/So = 0.10). Inhibitory activity (mean Sa = 14-24 pmol/well; mean Sa/So = 0.17-0.28) of the masa and tortilla chip extracts were reduced > or = 80% compared to the corn extract. Results were corroborated in a second experiment in which Sa and Sa/So of the wells treated with masa or tortilla chip extracts were reduced > or = 89% compared to those treated with the corn extract. Masa and tortilla chip FB1 concentrations (4-7 ppm) were reduced about 80-90% compared to the corn (30 ppm) when the materials were analyzed by high-performance liquid chromatography (HPLC). Therefore, nixtamalization reduced both the measured amount of FB1 and the ceramide synthase inhibitory activity of masa and tortilla chips extracts. The results further suggest that the masa and tortilla chip extracts did not contain significant amounts of unknown fumonisins having ceramide synthase inhibitory activity.
Voss
Kenneth A
KA
Toxicology and Mycotoxin Research Unit, USDA Agricultural Research Service, Athens, Georgia 30605-5677, USA. kvoss@saa.ars.usda.gov
Norred
William P
WP
Meredith
Filmore I
FI
Riley
Ronald T
RT
Stephen Saunders
D
D
D000818:Animals; D001681:Biological Assay; D002522:Chlorocebus aethiops; D005433:Flour / Q000382:microbiology; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D005511:Food Handling; D005516:Food Microbiology; D037341:Fumonisins / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D010088:Oxidoreductases / Q000378:metabolism*; D014709:Vero Cells; D003313:Zea mays / Q000737:chemistry* / Q000382:microbiology
D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1; D010088:Oxidoreductases; C108138:dihydroceramide desaturase