[Cloning and sequencing of the GTP cyclohydrolase I function region of azole target enzyme gene in artificially induced resistant Candida albicans].
OBJECTIVE
To study the relationship between gene mutation of GTP cyclohydrolase I function region in lanosterine 14 alpha-demethylase (14-DM) DNA sequence and drug resistance of Candida albicans.


METHODS
One standard strain and 2 isolate strains of Candida albicans were induced artificially by fluconazole plus albendazole. The gene fragments of the 3 strains and another 2 clinical isolates which were resistant to fluconazol were detected by PCR, and then cloned onto pMD-18T vectors to sequence and analyze the change of gene sequence after the induction.


RESULTS
The sequences underwent substantial gene mutations after induction. Some of the mutations resulted in alteration of amino acids. The sequence change and subsequent alteration of amino acids in the tested strains coincided with those in the clinical isolates.


CONCLUSIONS
Gene mutation and alteration of amino acid of 14-DM GTP domain are related to azole-resistance in Candida albicans.
Deng
Xu-liang
XL
Peking University School and Hospital of Stomatology, Beijing 100081, China.
Zhang
Xin-cheng
XC
Zhang
Feng
F
Li
Ruo-yu
RY
Lu
Hui-jun
HJ
He
Wen-qi
WQ
Liu
Li-guo
LG
Fan
Bin
B
Hu
Xiao-yang
XY
D015766:Albendazole / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology; D006136:GTP Cyclohydrolase / Q000235:genetics*; D009154:Mutation / Q000187:drug effects; D017422:Sequence Analysis, DNA
D000935:Antifungal Agents; D004271:DNA, Fungal; D015725:Fluconazole; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases; D006136:GTP Cyclohydrolase; D015766:Albendazole