[Application of multiparameter flow cytometry in diagnosis of non-Hodgkin lymphoma].
OBJECTIVE
To evaluate the role and application of flow cytometry in the diagnosis of non-Hodgkin lymphoma (NHL).


METHODS
Fresh cell samples from 40 cases of lymphoproliferative disorders were obtained by fine needle aspiration or excisional biopsies. Multiparameter flow cytometry was used to study the surface antigens of lymphoid cells. The immunophenotyping results were also correlated with morphologic features seen in the cytology preparations.


RESULTS
Of the 40 cases with histologic diagnosis of NHL, 37 cases (92.5%) had the lymphoma diagnosis confirmed by this method. The concordance rate for the 20 cases of B-cell NHL was 100%. As for the 17 cases with histologic diagnosis of T-cell NHL, 12 cases (66.7%) were correctly diagnosed as T-cell NHL using flow cytometry, while 2 cases (11.8%) were interpreted as B-cell NHL and the remaining 3 cases (17.6%) were undiagnosed.


CONCLUSIONS
Immunophenotyping by flow cytometry can serve as an ancillary technique in diagnosis and subclassification of NHL.
He
Fu-jin
FJ
Department of Pathology, School of Oncology, Peking Cancer Hospital, Peking University, Beijing 100036, China. fujinhe@sina.com
Xie
Yan
Y
Li
Ji-you
JY
Yong
Wei-ben
WB
Lu
Ai-ping
AP
Zhu
Jun
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D044963:Biopsy, Fine-Needle; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity