Microtargeted gene silencing and ectopic expression in live embryos using biolistic delivery with a pneumatic capillary gun.
Shefi
Orit
O
Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0376, USA. oshefi@ucsd.edu
Simonnet
Claire
C
Baker
Michael W
MW
Glass
James R
JR
Macagno
Eduardo R
ER
Groisman
Alex
A
D000199:Actins / Q000235:genetics; D000818:Animals; D019470:Biolistics / Q000295:instrumentation; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian / Q000502:physiology*; D004867:Equipment Design; D005456:Fluorescent Dyes; D015870:Gene Expression; D020868:Gene Silencing; D047709:Hirudo medicinalis / Q000196:embryology; D009414:Nerve Growth Factors / Q000235:genetics; D034622:RNA Interference; D013194:Staining and Labeling; D014162:Transfection
D000199:Actins; D005456:Fluorescent Dyes; D009414:Nerve Growth Factors