Expression of endo-1,4-beta-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening.
Arabidopsis thaliana CEL1 protein was detected in young expanding tissues. Immunostaining revealed that CEL1 accumulated mostly in xylem cells. The primary, as well as the secondary xylem showed considerable CEL1 staining. CEL1 was also observed in young epidermal cells, in which the thicker lateral and tangential walls stained more intensely than the inner walls. In newly formed cell walls, the lateral tangential walls were labeled more intensively than the inner walls. Cellulase activity was found to be significantly higher in growing tissue compared to mature parts of the plant. Cel1 expression concurrently with cellulase activity could be restored in detached matured leaves by sucrose treatment after 48 h in the culture medium.
Shani
Ziv
Z
CBD-Technologies Ltd., Tamar Science Park, PO Box 199, Rehovot, 76100, Israel.
Dekel
Mara
M
Roiz
Levava
L
Horowitz
Miri
M
Kolosovski
Natalia
N
Lapidot
Shaul
S
Alkan
Sari
S
Koltai
Hinanit
H
Tsabary
Galit
G
Goren
Raphael
R
Shoseyov
Oded
O
D000906:Antibodies / Q000276:immunology; D017360:Arabidopsis / Q000033:anatomy & histology* / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002473:Cell Wall / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D018547:Plant Stems / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D021381:Protein Transport / Q000187:drug effects; D013395:Sucrose / Q000494:pharmacology
D000906:Antibodies; D029681:Arabidopsis Proteins; D013395:Sucrose; D005966:Glucuronidase; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase