title: Binding of a transition state analog to newly synthesized Rubisco.
abstract: Radioactive amino acids, when added to isolated pea chloroplasts or chloroplast extracts engaged in protein synthesis, are incorporated into Rubisco large subunits that co-migrate with native Rubisco during nondenaturing electrophoresis. We have added the transition state analog 2'-carboxyarabinitol bisphosphate (CABP) to chloroplast extracts after in organello or in vitro incorporation of radioactive amino acids into Rubisco large subunits. Upon addition of CABP the radioactive bands co-migrating with native Rubisco undergo a readily detected shift in electrophoretic mobility just as the native enzyme, thus demonstrating the ability of the newly assembled molecules to interact with this transition state analog.
authors.affiliation: Biology Department, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, USA.
authors.forename: Boovaraghan
authors.initials: B
authors.lastname: Balaji
authors.forename: Michele
authors.initials: M
authors.lastname: Gilson
authors.forename: Harry
authors.initials: H
authors.lastname: Roy
mesh_terms: D018532:Pisum sativum / Q000378:metabolism; D010428:Pentosephosphates / Q000378:metabolism*; D011485:Protein Binding; D021122:Protein Subunits; D011868:Radioisotopes; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis* / Q000378:metabolism*; D013402:Sugar Alcohols / Q000378:metabolism*
chemical_list: D010428:Pentosephosphates; D021122:Protein Subunits; D011868:Radioisotopes; D013402:Sugar Alcohols; C032687:2-carboxyarabinitol 1,5-biphosphate; D012273:Ribulose-Bisphosphate Carboxylase