title: Ultrastructural study of myxoma virus morphogenesis.
abstract: Poxviruses are among the largest and most complex viruses known. Vaccinia virus, the prototype of the family Poxviridae, has been studied much more than myxoma virus. The aim of this work was to have a better knowledge about myxoma virus morphogenesis. The characterization of the main stages of MV morphogenesis was achieved by ultrastructural and immunological analysis. Specific antibodies were raised against M022L and M071L, two envelope proteins of extracellular enveloped virus and intracellular mature virus, respectively. The main stages of assembly were similar to those seen with other poxviruses, and the duration of the whole replication cycle was estimated to be around 16 h, longer than what was described for vaccinia virus. Morphological changes of infected cells were associated with the development of long cellular projections and enlarged microvilli. Intracellular enveloped viruses are associated with the cytoskeleton to move through the cell. Unlike earlier studies, as many cell-associated enveloped viruses as intracellular enveloped viruses were observed in relation with specialized microvilli, although these structures were rarely noticed. Finally, an unusual spreading process was observed, which uses cytoplasmic corridors.
authors.affiliation: UMR 1225 Interactions Hôtes-Agents pathogènes, INRA/ENVT, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France.
authors.forename: J-L
authors.initials: JL
authors.lastname: Duteyrat
authors.forename: J
authors.initials: J
authors.lastname: Gelfi
authors.forename: S
authors.initials: S
authors.lastname: Bertagnoli
mesh_terms: D000818:Animals; D002460:Cell Line; D007668:Kidney / Q000166:cytology / Q000821:virology; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D009024:Morphogenesis; D009233:Myxoma virus / Q000254:growth & development* / Q000502:physiology / Q000648:ultrastructure*; D011817:Rabbits; D014779:Virus Replication