title: [Genetic monitoring of the Crimean-Congo Hemorrhagic Fever virus in Kazakhstan and Tajikistan in 2001-2003].
abstract: Blood specimens obtained from 32 CCHF patients were tested for the presence of CCHF virus markers. In addition, 3210 ticks of the genera Hyalomma asiaticum, Hyalomma anatolicum, and Dermacentor niveus were examined to identify the CCHF virus antigen and RNA. This material was obtained during the 2001-2003 local outbreaks of CCHF in Kazakhstan and Tajikistan. The nucleotide sequence in the region 983-1282 of S segment of the CCHF virus for 12 wild type strains was determined. The phylogenetic relationships among the established biovariants of CCHF virus, and also between these biovariants and those from other regions of the world were identified. We were the first to demonstrate the presence of an African-like genotype of CCHF virus in the territory of Kazakhstan. The conclusion was made that two genotypes of CCHF virus were in circulation in Kazakhstan. It was also demonstrated that CCHF virus, circulating in the territories of Kazakhstan and Tajikistan, was genetically heterogeneous.
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Tumanova
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: O I
authors.initials: OI
authors.lastname: Vyshemirskiĭ
authors.forename: V V
authors.initials: VV
authors.lastname: Gutorov
authors.forename: I D
authors.initials: ID
authors.lastname: Petrova
authors.forename: G I
authors.initials: GI
authors.lastname: Tiunnikov
authors.forename: K S
authors.initials: KS
authors.lastname: Ospanov
authors.forename: S V
authors.initials: SV
authors.lastname: Kazakov
authors.forename: S
authors.initials: S
authors.lastname: Karimov
authors.forename: F Kh
authors.initials: FKh
authors.lastname: Tishkova
authors.forename: A I
authors.initials: AI
authors.lastname: Pirov
authors.forename: S V
authors.initials: SV
authors.lastname: Netesov
authors.forename: V S
authors.initials: VS
authors.lastname: Petrov
mesh_terms: D000818:Animals; D001483:Base Sequence; D004196:Disease Outbreaks; D004784:Environmental Monitoring; D062665:Epidemiological Monitoring; D014644:Genetic Variation; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006479:Hemorrhagic Fever, Crimean / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D026863:Ixodidae / Q000821:virology; D007623:Kazakhstan / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis* / Q000097:blood / Q000235:genetics; D013620:Tajikistan / Q000453:epidemiology
chemical_list: D012367:RNA, Viral