[Structure analysis of apolipoprotein B 3' variable number of tandem repeats].
OBJECTIVE
To study the distribution, frequency and structure of variable number of tandem repeats (VNTR) in 3' region of apoB gene in Chinese population.


METHODS
Genomic DNA was extracted from peripheral blood obtained under consent from randomly-chosen 522 individuals who came to the hospital for physical examination, and used to screen for polymorphisms of 3' VNTR of the apoB gene by employing polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), gradient polyacrylamide gel electrophoresis, cloning and sequencing.


RESULTS
Sixteen types of alleles of apoB 3oVNTR were identified, among which heterozygotes were more than homozygotes. The biggest allele is HVE58, and the smallest one is HVE22. HVE34 had the highest frequency (40.4%), followed by HVE32 (34.7%). This showed significant difference from the allelic distribution of other populations (Caucasian and Swedish). Through sequencing of 60 alleles, a new isomer (Y-A=ATAATTAAATATTT) and four new types of alleles were found.


CONCLUSIONS
The Chinese population we studied had a higher frequency of small alleles and showed a difference in allelic structure and frequency distribution from European and American in this populations.
Du
Gong
G
Laboratory of Molecular Biology, Research Center of Clinical Medicine, Second Affiliated Hospital, Jinan University School of Medicine, Shenzhen People's Hospital, Shenzhen, Guangdong, 518020 PR China.
Xue
Hong
H
Wu
Gang
G
Zeng
Wu-wei
WW
Chen
Bao-sheng
BS
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001055:Apolipoproteins B / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D002681:China; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction
D001055:Apolipoproteins B