[Protective effect of maternal immunoglobulin G against NMDA-induced neurotoxicity on hippocampus neurons].
OBJECTIVE
To investigate possible protective effect of maternal immunoglobulin G (IgG) against N-methyl-D-aspartate-mediated neurotoxicity on primary-cultured rat hippocampal neurons and the mechanism of the effect.


METHODS
An in vitro system had been developed for the study of hippocampal neurons. Intracellular lactic dehydrogenase (LDH) release was used as a marker to measure the rates of neuronal damage. The cells were stained with Trypan blue to measure the rate of neuronal death.


RESULTS
N-methyl-D-aspartate (NMDA) at a concentration of 50 micromol/L resulted in increased release of LDH and the cell mortality (P < 0.01, respectively). Maternal IgG of different concentration (10 mg/L, 100 mg/L) inhibited NMDA-induced intracellular LDH release (P < 0.01, respectively) and cell mortality (P < 0.05, 0.01, respectively), and larger dose had stronger effect (P < 0.05).


CONCLUSIONS
Maternal IgG had protective effect on primary-cultured rat hippocampal neurons injured by NMDA and the effect was dose-dependent.
Wang
Hong-mei
HM
Department of Neurology and Rehabilitation Unit, Beijing Children's Hospital, Capital University of Medical Sciences, Beijing 100045, China.
Zou
Li-ping
LP
Zhang
Wei-hua
WH
Zeng
Min
M
Zhao
Chun-li
CL
Zhang
Jun-yan
JY
D000818:Animals; D000831:Animals, Newborn; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018690:Excitatory Amino Acid Agonists; D005260:Female; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007112:Immunity, Maternally-Acquired / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D007155:Immunologic Factors / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000096:biosynthesis; D008297:Male; D016202:N-Methylaspartate; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009924:Organ Culture Techniques; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D018690:Excitatory Amino Acid Agonists; D007074:Immunoglobulin G; D007155:Immunologic Factors; D016202:N-Methylaspartate; D007770:L-Lactate Dehydrogenase