Effect of recombinant human tissue inhibitor of matrix metalloproteinase-1 in rabbit mandibular distraction osteogenesis: a histological and immunohistochemical study.
BACKGROUND
Bone matrix metalloproteinases are capable of degrading bone matrix during the remodelling, and their degradation activities can be down regulated by the tissue inhibitors of matrix metalloproteinases. This study evaluated the influence of exogenous tissue inhibitor of matrix metalloproteinase-1 on the expression of matrix metalloproteinases and endogenous tissue inhibitor of matrix metalloproteinases in mandibular distraction osteogenesis.


METHODS
Fifteen New Zealand white rabbits were assigned to three groups: a negative control; a sham control group implanted with a collagen sheet; and an experimental group implanted with recombinant human tissue inhibitor of matrix metalloproteinase-1 impregnated in a collagen sheet. Rabbits were sacrificed at 6 weeks, 12 weeks and 24 weeks of consolidation.


RESULTS
Major expression of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases was observed at the early stage of consolidation, only positive signals of tissue inhibitors of matrix metalloproteinases were observed at 24 weeks. The addition of recombinant human tissue inhibitor of matrix metalloproteinases-1 did not affect bone maturation and remodelling.


CONCLUSIONS
An equilibrium of bone formation and resorption was reached at 24 weeks of consolidation in the rabbit mandible. No obvious influence of recombinant human tissue inhibitor of matrix metalloproteinase-1 on bone remodelling of mandibular distraction osteogenesis was noted.
Zheng
Li Wu
LW
Discipline of Oral & Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, China.
Ma
Li
L
Rabie
A Bakr M
AB
Cheung
Lim Kwong
LK
D000818:Animals; D016723:Bone Remodeling / Q000187:drug effects*; D006801:Humans; D008334:Mandible / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects*; D023421:Models, Animal; D019857:Osteogenesis, Distraction / Q000379:methods*; D011480:Protease Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000494:pharmacology*
D011480:Protease Inhibitors; D019715:Tissue Inhibitor of Metalloproteinase-1; D020781:Matrix Metalloproteinase 1