title: Synthesis and in vitro biocompatibility of injectable polyurethane foam scaffolds.
abstract: The development of therapeutics for orthopedic clinical indications exploiting minimally invasive surgical techniques has substantial benefits, especially for treatment of fragility fractures in the distal radius of osteoporotics and vertebral compression fractures. We have designed six formulations of injectable polyurethane foams to address these clinical indications. The polyurethanes were prepared by mixing two liquid components and injecting the reactive liquid mixture into a mold where it hardens in situ. Porous polyurethane foams were synthesized from lysine methyl ester diisocyanate, a poly(epsilon-caprolactone-co-glycolide) triol, a tertiary amine catalyst, anionic and non-ionic stabilizers, and a fatty acid pore opener. The rise time of the foams varied from 8-20 min. The porosity was approximately 95% and the pores varied in size from 100-1000 microm. The polyurethane foams supported attachment of viable (>95%) MG-63 cells under dynamic seeding conditions. We anticipate compelling opportunities will be available as a consequence of the favorable biological and physical properties of the injectable polyurethane foams.
authors.affiliation: Department of Chemical Engineering, Vanderbilt University, Nashville, Tennessee 37235, USA. scott.guelcher@vanderbilt.edu
authors.forename: Scott A
authors.initials: SA
authors.lastname: Guelcher
authors.forename: Vishal
authors.initials: V
authors.lastname: Patel
authors.forename: Katie M
authors.initials: KM
authors.lastname: Gallagher
authors.forename: Susan
authors.initials: S
authors.lastname: Connolly
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Didier
authors.forename: John S
authors.initials: JS
authors.lastname: Doctor
authors.forename: Jeffrey O
authors.initials: JO
authors.lastname: Hollinger
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D018786:Bone Substitutes / Q000138:chemical synthesis* / Q000737:chemistry; D002460:Cell Line; D050815:Fractures, Compression / Q000628:therapy; D006801:Humans; D008049:Lipase / Q000138:chemical synthesis* / Q000737:chemistry; D008422:Materials Testing; D010024:Osteoporosis / Q000628:therapy; D016062:Porosity; D016103:Spinal Fractures / Q000628:therapy
chemical_list: D001426:Bacterial Proteins; D018786:Bone Substitutes; C408182:pueA protein, Pseudomonas chlororaphis; D008049:Lipase