Observing S-phase dynamics of histone modifications with fluorescently labeled antibodies.
Histone modifications are central to epigenetic regulation and must be reestablished with each round of DNA replication. Here we describe methods to localize these modifications within mammalian nuclei and to relate them to specific spatiotemporal patterns of DNA replication.
Wu
Rong
R
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA.
Terry
Anna V
AV
Gilbert
David M
DM
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D019175:DNA Methylation; D004261:DNA Replication; D000431:Ethanol / Q000737:chemistry; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006657:Histones / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D016196:S Phase
D000906:Antibodies; D005456:Fluorescent Dyes; D006657:Histones; D000431:Ethanol; D001973:Bromodeoxyuridine