TRAIL gene reorganizes the cytoskeleton and decreases the motility of human leukemic Jurkat cells.
TRAIL can selectively induce rapid apoptosis of various types of tumor cells. We induced the expression of TRAIL in Jurkat cells, and measured the adhesion of cells to human umbilical vein endothelial cells (HUVECs) and laminin (LN) in a parallel plate flow chamber system and by using a colorimetric method. The apoptosis percentage, cycle distribution, intracellular Ca(2+) concentration, and adhesion molecule expression of the cells were detected by flow cytometry. Cytoskeleton was observed with a laser confocal microscopy. The roles of adhesion molecules in the cell interaction was defined by their function blocking. The results showed that TRAIL attenuated the adhesion of Jurkat cells to HUVECs and LN, as well as their transendothelial migration. The increased apoptosis and G1-phase cell percentages, decreased intracellular Ca(2+) concentration, depolymerized actin and impaired cell deformability could contribute to the decreased adhesion of Jurkat cells caused by TRAIL. Furthermore, CD11a was found to play a more important role than CD62L in the adhesion of Jurkat cells to HUVECs. These findings contribute to the knowledge on the role of TRAIL in tumor metastasis and provide mechanistic basis for the clinical application of TRAIL and tumor therapy.
Jiang
Yuhui
Y
Department of Biophysics, Health Science Center, Peking University, Beijing, People's Republic of China.
Chen
Kai
K
Tang
Zhiyu
Z
Zeng
Zhu
Z
Yao
Weijuan
W
Sun
Dagong
D
Ka
Weibo
W
He
Dongqi
D
Wen
Zongyao
Z
Chien
Shu
S
D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D040881:CD11a Antigen / Q000096:biosynthesis; D018801:CD2 Antigens / Q000096:biosynthesis; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002465:Cell Movement / Q000235:genetics* / Q000502:physiology; D003599:Cytoskeleton / Q000378:metabolism* / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D006801:Humans; D019169:Jurkat Cells; D019041:L-Selectin / Q000096:biosynthesis; D007797:Laminin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D053221:TNF-Related Apoptosis-Inducing Ligand; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
D051017:Apoptosis Regulatory Proteins; D040881:CD11a Antigen; D018801:CD2 Antigens; D015815:Cell Adhesion Molecules; D007797:Laminin; D008562:Membrane Glycoproteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha; D019041:L-Selectin; D002118:Calcium