title: Variation in Trypanosoma cruzi clonal composition detected in blood patients and xenodiagnosis triatomines: implications in the molecular epidemiology of Chile.
abstract: To identify Trypanosoma cruzi clones from chronically infected individuals, they were transferred to triatomines by the xenodiagnosis test (XD) with Triatoma infestans. Polymerase chain reaction (PCR) and hybridization assays were performed to detect minicircle DNA in human blood samples and triatomine feces, using probes to determine the T. cruzi clones present. T. cruzi clone 19 (TcI) resulted the most prevalent in humans, with a frequency of 0.70 compared with a frequency of 0.53 in triatomines. T. cruzi clone 39 (TcIId) was the most prevalent in T. infestans, with a frequency of 0.65 compared with 0.33 in humans. The T. cruzi clone 43 (TcIIe) was not detected in blood samples; nevertheless, it was present at a rate of 0.17 in T. infestans feces. In conclusion, the T. cruzi clones are associated to each host, suggesting that selective amplification of clones occurs in human and triatomines.
authors.affiliation: Program of Cellular and Molecular Biology, Biomedical Sciences Institute, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Ximena
authors.initials: X
authors.lastname: Coronado
authors.forename: Ines
authors.initials: I
authors.lastname: Zulantay
authors.forename: Helena
authors.initials: H
authors.lastname: Albrecht
authors.forename: Marlene
authors.initials: M
authors.lastname: Rozas
authors.forename: Werner
authors.initials: W
authors.lastname: Apt
authors.forename: Sylvia
authors.initials: S
authors.lastname: Ortiz
authors.forename: Jorge
authors.initials: J
authors.lastname: Rodriguez
authors.forename: Gittith
authors.initials: G
authors.lastname: Sanchez
authors.forename: Aldo
authors.initials: A
authors.lastname: Solari
mesh_terms: D000328:Adult; D000818:Animals; D014355:Chagas Disease / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission; D002677:Chile / Q000453:epidemiology; D002999:Clone Cells / Q000145:classification; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes; D018105:DNA, Kinetoplast / Q000097:blood / Q000302:isolation & purification; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods / Q000592:standards*; D016133:Polymerase Chain Reaction / Q000379:methods; D014225:Triatominae / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics*; D020525:Xenodiagnosis / Q000379:methods
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D018105:DNA, Kinetoplast