Determination of amikacin in body fluid by high-performance liquid-chromatography with chemiluminescence detection.
A simple and sensitive method was developed for the quantification of amikacin in human plasma and urine samples. The method involves centrifugation of body fluid plasma after dilution with an ethanol/sodium carbonate mixture, and then an aliquot of the supernatant is directly injected into the chromatograph. After separation on a reversed-phase C18 column (runtime 20 min), aminoglycoside is detected on the basis of its complex formation reaction with Cu(II), the catalyst of the luminol/hydrogen peroxide chemiluminescence system. Using a volume of 500 microl biological sample, linearity is established over the concentration range 0.15-2.0 microg/ml and the limit of detection (LOD) is ca. 50 microg/l in plasma or urine. The intra-day and inter-day precision (measured by relative standard deviation, R.S.D.%) are always less than 9%, and relative recoveries are found to be over 92%.
Serrano
Juan Manuel
JM
Department of Analytical Chemistry, Marie-Curie Building Annex , Rabanales Campus, University of Cordoba, E-14071 Cordoba, Spain.
Silva
Manuel
M
D000583:Amikacin / Q000032:analysis* / Q000097:blood / Q000652:urine; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D049449:Luminescence; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D000583:Amikacin