title: The Arabidopsis root hair cell wall formation mutant lrx1 is suppressed by mutations in the RHM1 gene encoding a UDP-L-rhamnose synthase.
abstract: Cell and cell wall growth are mutually dependent processes that must be tightly coordinated and controlled. LRR-extensin1 (LRX1) of Arabidopsis thaliana is a potential regulator of cell wall development, consisting of an N-terminal leucine-rich repeat domain and a C-terminal extensin-like domain typical for structural cell wall proteins. LRX1 is expressed in root hairs, and lrx1 mutant plants develop distorted root hairs that often swell, branch, or collapse. The aberrant cell wall structures found in lrx1 mutants point toward a function of LRX1 during the establishment of the extracellular matrix. To identify genes that are involved in an LRX1-dependent developmental pathway, a suppressor screen was performed on the lrx1 mutant, and two independent rol1 (for repressor of lrx1) alleles were isolated. ROL1 is allelic to Rhamnose Biosynthesis1, which codes for a protein involved in the biosynthesis of rhamnose, a major monosaccharide component of pectin. The rol1 mutations modify the pectic polysaccharide rhamnogalacturonan I and, for one allele, rhamnogalacturonan II. Furthermore, the rol1 mutations cause a change in the expression of a number of cell wall-related genes. Thus, the lrx1 mutant phenotype is likely to be suppressed by changes in pectic polysaccharides or other cell wall components.
authors.affiliation: Institute of Plant Biology, University of Zürich, 8008 Zürich, Switzerland.
authors.forename: Anouck
authors.initials: A
authors.lastname: Diet
authors.forename: Bruce
authors.initials: B
authors.lastname: Link
authors.forename: Georg J
authors.initials: GJ
authors.lastname: Seifert
authors.forename: Barbara
authors.initials: B
authors.lastname: Schellenberg
authors.forename: Ulrich
authors.initials: U
authors.lastname: Wagner
authors.forename: Markus
authors.initials: M
authors.lastname: Pauly
authors.forename: Wolf-Dieter
authors.initials: WD
authors.lastname: Reiter
authors.forename: Christoph
authors.initials: C
authors.lastname: Ringli
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D005109:Extracellular Matrix / Q000737:chemistry; D020869:Gene Expression Profiling; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D018517:Plant Roots / Q000166:cytology*; D012210:Rhamnose / Q000096:biosynthesis*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C427476:LRX1 protein, Arabidopsis; D009005:Monosaccharides; D014539:Uridine Diphosphate Sugars; D005964:Glucosyltransferases; C519657:RHM1 protein, Arabidopsis; D012210:Rhamnose