title: Sex-, stage- and tissue-specific regulation by a mosquito hexamerin promoter.
abstract: A portion of the 5'-flanking region of the female-specific hexamerin gene, Hex-1.2, from the mosquito Ochlerotatus atropalpus was used to drive expression of the luciferase reporter gene in Drosophila melanogaster. The proximal 0.7 kb of 5'-flanking DNA were sufficient to partially repress reporter gene activity in males and to drive tissue- and stage-specific expression comparable with that of the endogenous O. atropalpus Hex-1.2 gene. The Drosophila doublesex transcription factor (DSX), expressed in Escherichia coli, bound putative DSX sites of the Hex-1.2 gene differentially in vitro. Blocking expression of the female isoform of the Doublesex transcription factor in transgenic female flies resulted in reduction of luciferase expression to levels comparable with those in males, suggesting that Doublesex could contribute to regulation of female-specific expression of the O. atropalpus Hex-1.2 gene.
authors.affiliation: Department of Neurobiology and Developmental Sciences, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
authors.forename: U K
authors.initials: UK
authors.lastname: Jinwal
authors.forename: S O
authors.initials: SO
authors.lastname: Zakharkin
authors.forename: O V
authors.initials: OV
authors.lastname: Litvinova
authors.forename: S
authors.initials: S
authors.lastname: Jain
authors.forename: Helen
authors.initials: H
authors.lastname: Benes
mesh_terms: D024506:5' Flanking Region; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D024202:Electrophoretic Mobility Shift Assay; D005216:Fat Body / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D008018:Life Cycle Stages; D008156:Luciferases / Q000235:genetics; D008297:Male; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics; D044969:Ochlerotatus / Q000235:genetics* / Q000254:growth & development; D011401:Promoter Regions, Genetic; D012727:Sex Characteristics
chemical_list: D004268:DNA-Binding Proteins; C070369:DSX protein, Drosophila; D029721:Drosophila Proteins; D009687:Nuclear Proteins; C097162:Tra protein, Drosophila; D008156:Luciferases