title: Phosphorylation state-responsive lanthanide peptide conjugates: a luminescence switch based on reversible complex reorganization.
abstract: [reaction: see text] A luminogenic probe for peptide dephosphorylation has been developed. It consists of a serine-/tyrosine-containing peptide modified on the N-terminus with a tryptophan residue and a DTPA chelate capable of binding Tb(3+). We propose a mechanistic model for the luminescence enhancement based on the interconversion of monomeric and dimeric lanthanide species, which is affected by the phosphorylation state of the serine or tyrosine residue. The optical switch reports effectively on phosphatase-catalyzed dephosphorylation in vitro.
authors.affiliation: Department of Chemistry, Columbia University, New York, New York 10027, USA.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Tremblay
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Angel A
authors.initials: AA
authors.lastname: Martí
authors.forename: Joanne
authors.initials: J
authors.lastname: Dyer
authors.forename: Marlin
authors.initials: M
authors.lastname: Halim
authors.forename: Steffen
authors.initials: S
authors.lastname: Jockusch
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turro
authors.forename: Dalibor
authors.initials: D
authors.lastname: Sames
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000595:Amino Acid Sequence; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D008958:Models, Molecular; D004369:Pentetic Acid / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D010766:Phosphorylation; D012694:Serine / Q000737:chemistry; D013725:Terbium / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry
chemical_list: D028581:Lanthanoid Series Elements; D010455:Peptides; D013725:Terbium; D012694:Serine; D004369:Pentetic Acid; D014364:Tryptophan; D000469:Alkaline Phosphatase