Comparative 35S-sulfate and 3H-proline metabolism within the interdental septal bone and adjacent periodontal ligament.
Tooth movements require rapid remodeling of the periodontal ligament (PDL) and adjacent alveolar bone. Our objective was to compare the regional metabolism of sulfated-glycosaminoglycans (sGAG) within the PDL and adjacent alveolar bone and compare it to the metabolism of collagenous proteins using radioautographic techniques. Rats were injected with either (3)H-proline or (35)S-sulfate and maxillae were removed at 1, 6, and 12 hr 1-7 days later. Silver grains were counted over the PDL and adjacent alveolar bone and the incorporation and removal rates for each radioisotope were determined. In general, net collagenous protein incorporation and removal were greatest within the distal and net sGAG incorporation and removal were greatest within the mesial compartments of the periodontium. The rate of removal of (3)H-proline was significantly greater within the distal alveolar bone surface than the adjacent PDL at all levels (P < 0.001). In contrast, the rate of removal of (35)S-sulfate was significantly greater in the PDL than within the adjacent mesial surface of the interdental septum at all levels (P < 0.001). The mesial surfaces of the interdental septum had a slower rate of removal of both isotopes than distal surfaces at all levels (P < 0.001). Our data suggest significant regional differences in the metabolism of (35)S-sulfate and (3)H-proline within the PDL and alveolar bone, which likely result from the characteristics of the forces produced by the adjacent teeth and may be a factor in the remodeling of the alveolar wall coincident to tooth movement.
Johnson
Roger B
RB
Department of Periodontics and Preventive Sciences, University of Mississippi, Jackson, 39216, USA. rjohnson@sod.umsmed.edu
D000818:Animals; D008297:Male; D008334:Mandible / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D011392:Proline / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013462:Sulfur Radioisotopes / Q000378:metabolism; D014070:Tooth / Q000166:cytology / Q000378:metabolism; D014316:Tritium / Q000378:metabolism
D013462:Sulfur Radioisotopes; D014316:Tritium; D011392:Proline