Evaluation of alpha-D-mannopyranoside glycolipid micelles-lectin interactions by surface plasmon resonance method.
It is established that achieving higher binding affinities in carbohydrate-protein interactions requires multivalent presentations of the sugar ligands at the receptor binding site. Several inhibition, calorimetric, mass balance, and other studies have reiterated the beneficial effects of molecular level clustering of the sugar ligands for tight binding to the receptors. We have undertaken an effort to study the multivalent effects involving larger assemblies, represented by micelles, and their lectin interactions. The micelles were constituted with monomer bearing one- or two-sugar moieties at the monomolecular level and with varying the distances between the sugar moieties. Micellar aggregation studies and dynamic light scattering (DLS) studies afforded details of the aggregation numbers and the hydrodynamic diameters of various glycolipid (GL) micelles. The GL micelles were used as analytes of surface plasmon resonance (SPR) experiments on a lectin concanavalin A (Con A)-immobilized surface. SPR studies of the micelle-lectin interactions demonstrate that the ligand-receptor binding can be fit into the bivalent analyte model of interaction. Furthermore, micelles formed from two-sugar containing GLs are able to elicit favorable kinetic association rate constants in comparison to the micelles constituted with one-sugar containing GLs. The kinetic rate constants across the micelles and the effect of the sugar valencies in the GLs are discussed.
Murthy
Bandaru Narasimha
BN
School of Chemistry, Physics, and Earth Sciences, Flinders University, Adelaide 5001, Australia.
Voelcker
Nicolas Hans
NH
Jayaraman
Narayanaswamy
N
D003208:Concanavalin A / Q000737:chemistry* / Q000378:metabolism; D006017:Glycolipids / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008358:Mannose / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D008823:Micelles; D011485:Protein Binding; D020349:Surface Plasmon Resonance / Q000379:methods
D006017:Glycolipids; D008024:Ligands; D008823:Micelles; D003208:Concanavalin A; D008358:Mannose