Combinational numeral fingerprint spectra of Glycyrrhiza and analysis of common peak ratio invariableness in HPLC.
To overcome the instability of traditional Chinese medicine (TCM) fingerprint spectra (FPS) and to build up absolute identification standard of TCM, the construction method of combinational numeral fingerprint spectra (CNFPS) was set up. The analysis of invariableness based on CNFPS was carried out. It can be used as absolute quantitative standard to identify the difference of TCM samples. According to this method, the HPLC FPS of components extracted from Glycyrrhiza root samples by ethanol was analyzed. Stable results and accurate quality evaluation were obtained perfectly. The common peak ratio invariableness is an absolute standard in identifying TCM samples, which is better than the similarity method of the FPS.
Zou
Huabin
H
School of Chemistry and Chemical Engineering, Shandong University, Jinan, People's Republic of China.
Yang
Guosheng
G
Du
Aiqin
A
Yuan
Jiurong
J
Qin
Zhengran
Z
Xia
Yingying
Y
Aboul-Enein
Hassan Y
HY
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006035:Glycyrrhiza / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results