FKBP133: a novel mouse FK506-binding protein homolog alters growth cone morphology.
FK506-binding proteins are the peptidyl prolyl cis-trans isomerases that are involved in various intracellular events. We characterized a novel mouse FK506-binding protein homolog, FKBP133/KIAA0674, in the developing nervous system. FKBP133 contains a domain similar to Wiskott-Aldrich syndrome protein homology region 1 (WH1) and a domain homologous to FK506-binding protein motif. FKBP133 was predominantly expressed in cerebral cortex, hippocampus, and peripheral ganglia at embryonic day 18.5. FKBP133 protein was distributed in the axonal shafts and was partially co-localized with F-actin in the growth cones of dorsal root ganglion neurons (DRG). The number of filopodia was increased in the DRG neurons overexpressing FKBP133. In contrast, the overexpression of a mutant deleted the WH1 domain reduced the growth cone size and the number of filopodia. Furthermore, the neurons overexpressing FKBP133 became significantly resistant to Semaphorin-3A induced collapse response. These results suggest that FKBP133 modulates growth cone behavior with the WH1 domain.
Nakajima
Oumi
O
Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
Nakamura
Fumio
F
Yamashita
Naoya
N
Tomita
Yusuke
Y
Suto
Fumikazu
F
Okada
Takako
T
Iwamatsu
Akihiro
A
Kondo
Eisaku
E
Fujisawa
Hajime
H
Takei
Kohtaro
K
Goshima
Yoshio
Y
D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000378:metabolism; D020869:Gene Expression Profiling; D020439:Growth Cones / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology; D009474:Neurons / Q000166:cytology; D016415:Sequence Alignment; D022021:Tacrolimus Binding Proteins / Q000737:chemistry / Q000502:physiology*; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000737:chemistry / Q000502:physiology*
C512703:FKBP133 protein, mouse; D051300:Wiskott-Aldrich Syndrome Protein Family; D022021:Tacrolimus Binding Proteins