Technical note: a rapid pulsed-field gel electrophoresis method for analysis of bifidobacteria.
Pulsed-field gel electrophoresis (PFGE) is a widely used and highly discriminatory molecular typing method that has been applied to bifidobacteria. However, published PFGE protocols used with bifidobacteria require between 5 and 7 d to complete. A rapid PFGE method was developed that can be completed within 24 h.
Briczinski
E P
EP
Department of Food Science, The Pennsylvania State University, University Park 16802, USA.
Roberts
R F
RF
D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D016133:Polymerase Chain Reaction; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S