title: Efficient gene transfer into differentiated human trophoblast cells with adenovirus vector containing RGD motif in the fiber protein.
abstract: Previously we reported fiber-modified adenovirus (Ad) vectors containing the Arg-Gly-Asp (RGD) motif on the HI loop of the fiber knob (Ad-RGD vectors) have high gene transfer efficacy into some human trophoblast cell lines. In the current study, we investigate transgene activity of Ad-RGD during differentiation of human cytotrophoblast BeWo cells into syncytiotrophoblast-like cells. Although cellular differentiation into syncytiotrophoblast cells was followed by a decrease in the coxsackievirus and adenovirus receptor levels on the cell membrane, the alphaVbeta3 and alphaVbeta5 integrin levels did not change. Conventional adenovirus vector had lower transduction activity in the differentiated cells than non-differentiated cells. In contrast, Ad-RGD vector had no influence on differentiation and had a ca. 2-5 fold higher transduction activity than that of the conventional Ad vector. Thus, Ad-RGD vector can be a powerful tool for gene transfer experiments in syncytiotrophoblast cells.
authors.affiliation: Laboratory of Gene Transfer and Regulation, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Koizumi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
authors.forename: Masuo
authors.initials: M
authors.lastname: Kondoh
authors.forename: Makiko
authors.initials: M
authors.lastname: Fujii
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Naoki
authors.initials: N
authors.lastname: Utoguchi
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics*; D002454:Cell Differentiation; D002460:Cell Line; D000090702:Constitutive Androstane Receptor; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D009842:Oligopeptides / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D019030:Receptors, Vitronectin / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; D000090702:Constitutive Androstane Receptor; D039302:Integrin alphaVbeta3; D009842:Oligopeptides; D018160:Receptors, Cytoplasmic and Nuclear; D019030:Receptors, Vitronectin; C018315:hexon capsid protein, Adenovirus; C064660:integrin alphaVbeta5; C047981:arginyl-glycyl-aspartic acid