Changes of the cortex proteome and Apolipoprotein E in transgenic mouse models of Alzheimer's Disease.
Transgenic mice carrying human Amyloid Precursor Protein mutations present amyloid plaque deposition in the brain upon aging. In this study, we characterized the changes of cortex proteome and endogenous Apolipoprotein E in these mice. Differential analysis of two-dimensional electrophoresis images revealed spots altered upon aging, transgene addition and plaque deposition. Alpha-synuclein and cytochrome oxidase polypeptide Va were up-regulated in transgenic mice. Upon aging, expression of ATP synthase alpha, alpha enolase, UMP-CMP kinase, and dihydropyrimidinase like-2 protein was modified. These proteins and their modification probably play a role in the amyloid aggregate formation in these mice.
Carrette
O
O
Neuroproteomics Group, Department of Structural Biology and Bioinformatics, Geneva University, Faculty of Medicine, Geneva, Switzerland. Odile.Carrette@medecine.unige.ch
Burgess
J A
JA
Burkhard
P R
PR
Lang
C
C
Côte
M
M
Rodrigo
N
N
Hochstrasser
D F
DF
Sanchez
J-C
JC
D000544:Alzheimer Disease / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001057:Apolipoproteins E / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D020543:Proteome
D001057:Apolipoproteins E; D020543:Proteome