A high-performance liquid chromatography method for hypoxanthine determination in vitreous humour: application to estimation of post mortem interval.
We have developed a new technique to determine the concentration of hypoxanthine [Hx] in a reverse phase column using a modified high-performance liquid chromatography (HPLC) method that is faster and more reliable than those previously described. In this paper we present a formula for estimating the post mortem interval (PMI) based on this HPLC method by applying the inverse prediction method. The regression line obtained by changing the variables gives PMI = 0.183 [Hx] + 0.599 (PMI in hours, [Hx] in micromol/L, R2 = 0.531, P < 0.05).
Muñoz
J I
JI
Department of Pathology and Forensic Sciences, University of Santiago de Compostela, Spain.
Costas
E
E
Rodríguez-Calvo
M S
MS
Suárez-Peñaranda
J M
JM
López-Rivadulla
M
M
Concheiro
L
L
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005554:Forensic Medicine; D006801:Humans; D007042:Hypoxanthines / Q000032:analysis*; D011180:Postmortem Changes; D015203:Reproducibility of Results; D014822:Vitreous Body / Q000737:chemistry*
D007042:Hypoxanthines