title: [A related analysis for alpha, beta fibrinogen gene haplotypes and nucleotide polymorphisms associated with the ischemic stroke in Hainan Han population].
abstract: OBJECTIVE
  To analyze the association of that the polymorphisms and haplotypes of Taq I site in beta fibrinogen gene and the single nucleotide sites -455 G/A, -249 C/T, -148 C/T, +1689T/G, Bsm A I G/C, 448 G/A, Bcl I G/A, Hinf I A/C in beta-fibrinogen gene are linked up with the ischemic stroke(IS).
  
  
  METHODS
  Turbidmetric assay was used to measure the plasma fibrinogen level of one hundred and sixty cases with ischemic stroke and one hundred and thirty healthy individuals from Hainanese Han population. The polymorphisms and genotypes were characterized by PCR-RFLP. Hardy-Weinberg equilibrium and statistical differences of allelic, genotype and haplotype frequencies were obtained by Chi-square test. Pairwise linkage disequilibrium was calculated and haplotypes of nine or four polymorphisms were estimated by the EH + program.
  
  
  RESULTS
  There were highly significant differences in genotype frequencies and allelic frequencies of the polymorphisms -455 G/A, -148 C/T, 448 G/A, which happened between the IS group and control subjects (P< 0.01). However, the significant differences of the allelic frequencies in the other six polymorphisms were not found between the IS group and the control (P> 0.05). The odds ratio(OR) with the rare alleles of A -455, T -148 and A 448 is 2.46, 2.30 and 2.08 (95% confidence interval 1.153%-3.924%, 1.429%-3.694% and 1.298%-3.329%) respectively. No definite haplotype block was found by linkage disequilibrium analysis in the control group and the IS group. Association of haplotypes constructed from the nine polymorphisms with IS was not found. Among the haplotypes constructed from four polymorphisms including -455 G/A, -148 C/T, 448 G/A alleles, haplotype differences were found between the control group and the IS group. Haplotypes with G -455, C -148, G448 alleles appeared more frequently in control group(P< or = 0.01), whereas haplotypes with A -455, T -148, A 448 occurred more frequently in the IS group(P< 0.01).
  
  
  CONCLUSIONS
  The results of multi-allele and haplotype analysis indicated that the polymorphisms -455 G/A, -148 C/T, 448 G/A in beta fibrinogen gene were the possible risk factors associated with the occurrence of ischemic stroke in Hainan Han population.
authors.affiliation: Department of Biochemistry, Hainan Medical College, Haikou, Hainan, 571101 PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Liang
authors.forename: Chuan
authors.initials: C
authors.lastname: Sun
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Liao
authors.forename: Feng
authors.initials: F
authors.lastname: Xiao
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Chen
authors.forename: Shi-xiong
authors.initials: SX
authors.lastname: Huang
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Tang
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Wen
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Long
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Guo-xun
authors.initials: GX
authors.lastname: Liu
authors.forename: Shuang
authors.initials: S
authors.lastname: Cheng
authors.forename: Wang-wei
authors.initials: WW
authors.lastname: Cai
mesh_terms: D000483:Alleles; D002545:Brain Ischemia / Q000150:complications; D005260:Female; D005340:Fibrinogen / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D020521:Stroke / Q000209:etiology / Q000235:genetics*
chemical_list: D005340:Fibrinogen