Characterization of human dopamine responsive protein DRG-1 that binds to p75NTR-associated cell death executor NADE.
Expression of human dopamine responsive gene-1 (DRG-1) is up-regulated in response to treatment of dopamine in the rat astrocytes. However, its functions are not clear up to now. In the presented studies, DRG-1 was identified to be a conserved gene in the vertebrate and expressed abundantly in human testis, brain and skeletal muscle. DRG-1 was shown to interact with human p75NTR-associated cell death executor (NADE) in vivo and in vitro, and the interaction occurred in cytoplasm. The regions required for the interaction were subsequently mapped to the N-terminal of DRG-1 and the C-terminal of NADE. Furthermore, MTT assay showed that stable expression of DRG-1 in 293 cells could promote cell proliferation, and this promotion was suppressed by overexpression of NADE. In flow cytometry cell cycle analysis, overexpression of DRG-1 in 293 or PC12 cells increased the population of cells in the S phase with a concomitant decrease in G0/G1 population. These findings suggest that DRG-1 may contribute to the dopamine-induced cell growth, which is negatively regulated by NADE.
Yu
Yao
Y
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
Wang
Jiadong
J
Yuan
Hanying
H
Qin
Feng
F
Wang
Jing
J
Zhang
Nailing
N
Li
Yu-Yang
YY
Liu
Jianping
J
Lu
Hong
H
D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D019556:COS Cells; D002453:Cell Cycle / Q000235:genetics; D002455:Cell Division / Q000502:physiology; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D002874:Chromosome Mapping; D015536:Down-Regulation / Q000502:physiology; D005434:Flow Cytometry; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006824:Hybridization, Genetic; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D016716:PC12 Cells; D010957:Plasmids; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae; D013347:Subcellular Fractions; D013778:Tetrazolium Salts; D013844:Thiazoles; D014018:Tissue Distribution; D014162:Transfection
D051017:Apoptosis Regulatory Proteins; C410727:BEX3 protein, human; D013778:Tetrazolium Salts; D013844:Thiazoles; C078418:developmentally regulated GTP-binding protein; D019204:GTP-Binding Proteins; C022616:thiazolyl blue; D005978:Glutathione