The bcl-2 major breakpoint region (mbr) possesses transcriptional regulatory function.
The bcl-2 major breakpoint region (mbr), located within the 3'-UTR of the bcl-2 gene, is the site of the most common chromosomal translocation, t(14;18) (q32;q21), which occurs in follicular lymphoma. The mbr forms a triplex DNA structure under physiological conditions and the transcription factor special AT-rich sequence-binding protein 1 (SATB1) binds immediately downstream of the mbr. These observations raise the possibility that the mbr may be involved in regulation of bcl-2 gene expression. We investigated the role of the bcl-2 mbr on reporter gene activity and the relevance of SATB1 to this function in a variety of cell lines. We found that the mbr up-regulated reporter gene expression. Deletion of the 37-bp AT-rich SATB1 binding site abolished the bcl-2 mbr regulation of reporter gene expression. Overexpression of SATB1 enhanced bcl-2 mbr up-regulation of the reporter gene activity. Our data strongly demonstrated that the bcl-2 mbr possessed regulatory function that was related to SATB1.
Zhang
Jingjing
J
Key Laboratory of Human Functional Genomics of Jiangsu Province, PR China.
Ma
Changyan
C
Han
Xiao
X
Durrin
Linda K
LK
Sun
Yujie
Y
D020413:3' Untranslated Regions / Q000737:chemistry; D001665:Binding Sites; D045744:Cell Line, Tumor; D005260:Female; D017930:Genes, Reporter; D006801:Humans; D019169:Jurkat Cells; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008214:Lymphocytes; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D020413:3' Untranslated Regions; D036961:Matrix Attachment Region Binding Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; C076189:SATB1 protein, human; D008156:Luciferases