title: Inhibitory effects of Tabebuia impetiginosa inner bark extract on platelet aggregation and vascular smooth muscle cell proliferation through suppressions of arachidonic acid liberation and ERK1/2 MAPK activation.
abstract: The antiplatelet and antiproliferative activities of extract of Tabebuia impetiginosa inner bark (taheebo) were investigated using washed rabbit platelets and cultured rat aortic vascular smooth muscle cells (VSMCs) in vitro. n-Hexane, chloroform and ethyl acetate fractions showed marked and selective inhibition of platelet aggregation induced by collagen and arachidonic acid (AA) in a dose-dependent manner. These fractions, especially the chloroform fraction, also significantly suppressed AA liberation induced by collagen in [(3)H]AA-labeled rabbit platelets. The fractions, especially the chloroform fraction, potently inhibited cell proliferation and DNA synthesis induced by platelet derived growth factor (PDGF)-BB, and inhibited the levels of phosphorylated extracellular signal regulated kinase (ERK1/2) mitogen activated protein kinase (MAPK) stimulated by PDGF-BB, in the same concentration range that inhibits VSMC proliferation and DNA synthesis.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Dong-Ju
authors.initials: DJ
authors.lastname: Son
authors.forename: Yong
authors.initials: Y
authors.lastname: Lim
authors.forename: Young-Hyun
authors.initials: YH
authors.lastname: Park
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Chang
authors.forename: Yeo-Pyo
authors.initials: YP
authors.lastname: Yun
authors.forename: Jin-Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Gary R
authors.initials: GR
authors.lastname: Takeoka
authors.forename: Kwang-Geun
authors.initials: KG
authors.lastname: Lee
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Mi-Ran
authors.initials: MR
authors.lastname: Kim
authors.forename: Jeong-Han
authors.initials: JH
authors.lastname: Kim
authors.forename: Byeoung-Soo
authors.initials: BS
authors.lastname: Park
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects*; D024301:Plant Bark / Q000737:chemistry*; D028321:Plant Preparations / Q000737:chemistry / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D029663:Tabebuia / Q000737:chemistry*
chemical_list: D028321:Plant Preparations; D016718:Arachidonic Acid; D048049:Extracellular Signal-Regulated MAP Kinases