Determination of optimal conditions for studies of electrophoretic mobility of human erythrocytes loaded with cryoglobulins.
For studies of the charge characteristics of human erythrocytes loaded with cryoglobulins the cells should be stored in Alsever solution with 0.1% sodium azide no longer than 1 week after blood collection. Erythrocytes should be loaded with cryoglobulins by incubation during at least 4 h, the final concentration of cryoproteins in the solution being no lower than 30 microg/ml.
Konstantinova
N A
NA
Department of Experimental and Theoretical Physics, Russian State Medical University, Moscow. cryoglobulin@mail.ru
Karandashov
E N
EN
D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D003450:Cryoglobulins / Q000737:chemistry* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D019810:Sodium Azide / Q000494:pharmacology; D013048:Specimen Handling; D013997:Time Factors
D003450:Cryoglobulins; D019810:Sodium Azide