title: [The application of immunoperoxidase test for the detection of antibodies various classes (IgA, IgG and IgM) coating bacteria in urine].
abstract: For the detection of bacteria coated with immunoglobulins in urine the monoclonal antibodies against human IgA, IgG and IgM conjugated with peroxidase were used. For comparison, the immunofluorescence technique was also employed. The results obtained by two methods revealed that immunofluorescence were less sensitive. It was found that bacteria were predominantly coated with IgA (41,9 +/- 22,4%) and IgG (34,1 +/- 15,3 %) immunoglobulins. The IgM antibodies were found rarely (12,8 +/- 8%).
authors.affiliation: Zakład Diagnostyki Mikrobiologicznej Akademii Medycznej w Białymstoku.
authors.forename: Piotr
authors.initials: P
authors.lastname: Jakoniuk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Leszczyńska
authors.forename: Marta
authors.initials: M
authors.lastname: Zalewska
authors.forename: Paweł
authors.initials: P
authors.lastname: Sacha
authors.forename: Piotr
authors.initials: P
authors.lastname: Wieczorek
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000652:urine*; D001419:Bacteria / Q000276:immunology; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014556:Urine / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M