title: [Artificial cornea, cell culture and tissue engineering].
abstract: The cornea, the fibrous transparent layer of the eye ball, is becoming a target of tissue engineering research because of the scarcity of donors. We report our experience in developing a mechanism to build a full-thickness corneal substitute using cell culture and protocols of tissue engineering. Isolation of keratocytes, epithelial and endothelial cells was accomplished by culturing denuded corneal stroma, limbal explant and enzymatically detached endothelium. Each type of cell was cultured in selective medium. Microanalytical quality control of cell viability was carried out. A multilayered cornea construct was built by sequential deposition of endothelium, keratocytes immersed a gel of human fibrin and epithelium in a manner that reproduce the normal histological structure.
authors.forename: Antonio
authors.initials: A
authors.lastname: Campos Muñoz
mesh_terms: D001187:Artificial Organs; D002478:Cells, Cultured; D003315:Cornea; D006801:Humans; D023822:Tissue Engineering