title: [Autoantibodies against beta1 and M2 receptor in diabetic patients with refractory hypertension].
abstract: OBJECTIVE
  To explore the role of the autoantibodies against M(2)-muscarinic receptor (M(2)-receptor), beta(1)-adrenergic receptor (beta(1)-receptor) in the development of diabetic with refractory hypertension.
  
  
  METHODS
  Serum autoantibodies against M(2) and beta(1) were detected by ELISA using synthesized epitopes of the second extracellular loop of M(2) receptor (169 - 193) and beta(1) receptor (197 - 222) in healthy controls (n = 40), diabetic patients (n = 62), diabetic patients with non-refractory hypertension (n = 55) and diabetic patients with refractory hypertension (n = 81).
  
  
  RESULTS
  The positive rates of the autoantibodies against M(2) receptor and beta(1) receptor were similar among healthy controls (15.0% and 17. 5%), diabetes mellitus patients (17.7% and 14.5%) and diabetic patients with non-refractory hypertension (16.4% and 12.7%) but are significantly higher in diabetic patients with refractory hypertension (64.2% and 55.6%, P < 0.01 vs. other 3 groups).
  
  
  CONCLUSIONS
  This finding suggests that autoimmune mechanisms might play a role in the pathogenesis of diabetic patients with refractory hypertension.
authors.affiliation: Department of Endocrinology Wuhan General Hospital of GuangZhou Military Region, Wuhan 430070, China.
authors.forename: Lin-shuang
authors.initials: LS
authors.lastname: Zhao
authors.forename: Yu-hua
authors.initials: YH
authors.lastname: Liao
authors.forename: Guang-da
authors.initials: GD
authors.lastname: Xiang
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Zi-hua
authors.initials: ZH
authors.lastname: Zhou
authors.forename: Jie
authors.initials: J
authors.lastname: Hou
authors.forename: Ling
authors.initials: L
authors.lastname: Le
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000150:complications; D008297:Male; D008875:Middle Aged; D043585:Receptor, Muscarinic M2 / Q000276:immunology*; D018342:Receptors, Adrenergic, beta-1 / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D043585:Receptor, Muscarinic M2; D018342:Receptors, Adrenergic, beta-1