Immunohistochemical study of small integrin-binding ligand, N-linked glycoproteins in reactionary dentin of rat molars at different ages.
Small integrin-binding ligand, N-linked glycoproteins (SIBLING) are believed to play key roles in the process of biomineralization. Reactionary dentin (RD), formed by odontoblasts in response to external stimuli, differs morphologically from primary dentin (PD). To test our hypothesis that the microscopic changes reflect variations in molecular mechanisms involved in formation of the two forms of dentin, and to characterize RD further, we compared the distributions of four SIBLING proteins [bone sialoprotein (BSP), osteopontin (OPN), dentin matrix protein 1 (DMP-1) and dentin sialophosphoprotein (DSPP)] in naturally occurring RD with those in PD. Molars of rats aged 12, 18, 24 and 36 wk were analyzed using immunohistochemistry with antibodies against BSP, OPN, DMP-1, and dentin sialoprotein (a fragment of DSPP). Differences in the distribution of the four SIBLING proteins were evident. Bone sialoprotein, not seen in PD, was consistently observed in RD. Osteopontin, almost absent from PD, was clearly observed in RD. The expression levels of DMP-1 and DSP in RD were lower than in PD. Elevated expression of BSP and OPN, along with a marked decrease of dentin sialoprotein and DMP-1 in RD, suggests a difference in the mechanism of formation of the two forms of dentin.
Moses
Kyle D
KD
Department of Endodontics, University of Texas Health Science Center at Houston Dental Branch, Houston, 77030, USA.
Butler
William T
WT
Qin
Chunlin
C
D000367:Age Factors; D000818:Animals; D000906:Antibodies; D004396:Coloring Agents; D003782:Dental Pulp / Q000166:cytology; D003804:Dentin / Q000166:cytology; D003809:Dentin, Secondary / Q000166:cytology*; D003810:Dentinogenesis / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000032:analysis; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein; D008963:Molar / Q000166:cytology; D009804:Odontoblasts / Q000166:cytology; D053495:Osteopontin; D010750:Phosphoproteins / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000032:analysis*; D019217:Tooth Attrition / Q000473:pathology; D000099311:Dentin Sialophosphoprotein
D000906:Antibodies; D004396:Coloring Agents; D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D053495:Osteopontin; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C081635:Dmp1 protein, rat; C546873:Ibsp protein, rat; C506897:Spp1 protein, rat