Arginine-rich intracellular delivery peptides noncovalently transport protein into living cells.
Plasma membranes of plant or animal cells are generally impermeable to peptides or proteins. Many basic peptides have previously been investigated and covalently cross-linked with cargoes for cellular internalization. In the current study, we demonstrate that arginine-rich intracellular delivery (AID) peptides are able to deliver fluorescent proteins or beta-galactosidase enzyme into animal and plant cells, as well as animal tissue. Cellular internalization and transdermal delivery of protein could be mediated by effective and nontoxic AID peptides in a neither fusion protein nor conjugation fashion. Therefore, noncovalent AID peptides may provide a useful strategy to have active proteins function in living cells and tissues in vivo.
Wang
Ya-Hui
YH
Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
Chen
Chung-Pin
CP
Chan
Ming-Huan
MH
Chang
Microsugar
M
Hou
Yu-Wun
YW
Chen
Hwei-Hsien
HH
Hsu
Hui-Ru
HR
Liu
Kevin
K
Lee
Han-Jung
HJ
D000818:Animals; D001120:Arginine / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival; D002477:Cells / Q000166:cytology* / Q000378:metabolism*; D006224:Cricetinae; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism; D051379:Mice; D019697:Onions / Q000166:cytology / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity; D010957:Plasmids / Q000235:genetics; D021381:Protein Transport; D012867:Skin / Q000166:cytology / Q000378:metabolism
D010455:Peptides; D001120:Arginine