A sensitive method to monitor ectodomain shedding of ligands of the epidermal growth factor receptor.
All ligands of the epidermal growth factor receptor (EGFR) are made as membrane anchored precursors that can be proteolytically processed and released from the plasma membrane. This process, which is referred to as protein ectodomain shedding, is emerging as a key regulator of the function of EGFR ligands. In light of the important roles of EGFR signaling in development and disease, it will be important to understand more about the regulation of proteolytic processing of EGFR ligands. This chapter describes a sensitive and semiquantitative method to measure ectodomain shedding of EGFR ligands that was designed to facilitate studies of this process in cells.
Sahin
Umut
U
Arthritis and Tissue Degeneration Program, Hospital for Special Surgery, Weill Medical College of Cornell University, New York, NY, USA.
Weskamp
Gisela
G
Zheng
Yufang
Y
Chesneau
Valerie
V
Horiuchi
Keisuke
K
Blobel
Carl P
CP
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D066246:ErbB Receptors / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D008024:Ligands; D051379:Mice; D012680:Sensitivity and Specificity
D008024:Ligands; D066246:ErbB Receptors; D000469:Alkaline Phosphatase