title: Lipid fluorination enables phase separation from fluid phospholipid bilayers.
abstract: To probe the effect of lipid fluorination on the formation of lipid domains in phospholipid bilayers, several new fluorinated and non-fluorinated synthetic lipids were synthesised, and the extent of phase separation of these lipids from phospholipid bilayers of different compositions was determined. At membrane concentrations as low as 1% mol/mol, both fluorinated and non-fluorinated lipids were observed to phase separate from a gel-phase (solid ordered) phospholipid matrix, but bilayers in a liquid disordered state caused no phase separation; if the gel-phase samples were heated above the transition temperature, then phase separation was lost. We found incorporation of perfluoroalkyl groups into the lipid enhanced phase separation, to such an extent that phase separation was observed from cholesterol containing bilayers in the liquid ordered phase.
authors.affiliation: Manchester Interdisciplinary Biocentre, School of Chemistry, University of Manchester, UK. S.Webb@manchester.ac.uk
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Webb
authors.forename: Kevin
authors.initials: K
authors.lastname: Greenaway
authors.forename: Marzieh
authors.initials: M
authors.lastname: Bayati
authors.forename: Laurent
authors.initials: L
authors.lastname: Trembleau
mesh_terms: D006845:Hydrocarbons, Fluorinated / Q000138:chemical synthesis / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification*; D019906:Nuclear Magnetic Resonance, Biomolecular; D013696:Temperature
chemical_list: D006845:Hydrocarbons, Fluorinated; D008051:Lipid Bilayers; D008055:Lipids