title: Reduction of vitrification in in vitro raised shoots of Chlorophytum borivilianum Sant. & Fernand., a rare potent medicinal herb.
abstract: Reduction of vitrification in in vitro raised shoots derived from shoot bases and immature floral buds along with inflorescence axis used as explants of C. borivilianum, a rare medicinal herb is described. Shoot multiplication was obtained on MS medium with 2 mg l(-1) benzylaminopurine (BAP) + 0.1 mg l(-1) indole-3-butyric acid (IBA) and MS medium with 2 mg l(-1) kinetin (Kin) + 0.1 mg l(-1) 2,4-dichlorophenoxy acetic acid (2,4-D) from shoot bases and inflorescence axis respectively. Best multiplication rates were obtained from both the explants on MS medium with 2 mg l(-1) BAP. Vitrification of shoots in cultures appeared during the multiplication stage. Culture bottles with aerated caps reduced the vitrification to 80%. Reduction of BAP concentration from 2 mg l(-1) to zero during subsequent subcultures also minimized vitrification. Use of 0.5-2 mg l(-1) Kin produced healthy shoots when compared to BAP. In vitro raised shoots rooted on Knop salts containing iron and vitamins of MS medium, 2 mg l(-1) IBA and 0.1% activated charcoal. About 80% plantlets survived upon soil transfer. Scanning electron microscopic and image analyzer studies reveal the morphological structural differences between the leaves of normal and vitrified plantlets.
authors.affiliation: Department of Biosciences, Sardar Patel University, Vallabh Vidyanagar 388 120, India.
authors.forename: Urvashi
authors.initials: U
authors.lastname: Sharma
authors.forename: J S S
authors.initials: JS
authors.lastname: Mohan
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D001593:Benzyl Compounds / Q000494:pharmacology; D003583:Cytokinins / Q000378:metabolism; D029001:Herbal Medicine; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism*; D010946:Plants, Medicinal / Q000378:metabolism*; D011687:Purines / Q000494:pharmacology; D013997:Time Factors
chemical_list: D001593:Benzyl Compounds; D003583:Cytokinins; D007211:Indoles; D011687:Purines; C014612:indolebutyric acid; D015084:2,4-Dichlorophenoxyacetic Acid; C480551:benzylaminopurine; D007701:Kinetin