title: Characterization of a mutant strain of Rhodovulum sulfidophilum lacking the pufA and pufB genes encoding the polypeptides for the light-harvesting complex 1 (B 870).
abstract: Contradictory results on the effectiveness of energy transfer from the light harvesting complex 2 (LH2) directly to the reaction center (RC) in mutant strains lacking the core light-harvesting complex 1 (LH1) have been obtained with cells of Rhodobacter capsulatus and Rhodobacter sphaeroides. A LH1(-) mutant of Rhodovulum sulfidophilum, named rsLRI, was constructed by deletion of the pufBA genes, resulting in a kanamycin resistant photosynthetically positive clone. To restore the wild type phenotype, a complemented strain C2 was constructed by inserting in trans a DNA segment containing the pufBA genes. Light-induced FTIR difference spectra indicate that the RC in the rsLRI mutant and in the C2 complemented strains are functionally and structurally identical with those in the wild type strain, demonstrating that the assembly and the function of the RC is not impaired by the LH1 deletion. The photosynthetic growth rate of the rsLRI strain increased with decreasing light intensity. At 50 W m(-2 )no photosynthetic growth was observed. These results indicate that the light energy harvested by the LH2 complex was not or inefficiently transferred to the RC; thus most of the energy necessary for photosynthetic growth is in the LH1(-) strain directly absorbed by the RC. It is supposed that in the mutant strain, RC and LH2 cannot interact in an efficient way.
authors.affiliation: Cátedra de Microbiología, Facultad de Agronomía, UBA and Instituto de Investigaciones Bioquímicas y Fisiológicas (IByF-CONICET), Av. San Martín 4453, 1417, Capital Federal, Argentina.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Raiger-Iustman
authors.forename: Norma L
authors.initials: NL
authors.lastname: Kerber
authors.forename: Norma L
authors.initials: NL
authors.lastname: Pucheu
authors.forename: Marc J
authors.initials: MJ
authors.lastname: Bornmann
authors.forename: Simon
authors.initials: S
authors.lastname: Kohler
authors.forename: Andreas
authors.initials: A
authors.lastname: Labahn
authors.forename: Monier
authors.initials: M
authors.lastname: Tadros
authors.forename: Gerhart
authors.initials: G
authors.lastname: Drews
authors.forename: Augusto F
authors.initials: AF
authors.lastname: García
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001429:Bacteriochlorophylls / Q000378:metabolism; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology; D005816:Genetic Complementation Test / Q000379:methods; D045342:Light-Harvesting Protein Complexes / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D016296:Mutagenesis / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D041903:Rhodovulum / Q000235:genetics* / Q000254:growth & development; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D010455:Peptides