Phellinsin A from Phellinus sp. PL3 exhibits antioxidant activities.
Phellinsin A, which was isolated from the culture broth of Phellinus sp. PL3, exhibited significant low-density lipoproteins (LDL)-antioxidant activity. It inhibited the Cu2+-mediated oxidation of LDL (IC50: 5.3 microM) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPH)-mediated oxidation of LDL (IC50: 2.8 microM) in the thiobarbituric acid-reactive substances (TBARS) assay as well as the macrophage-mediated LDL oxidation (73% inhibition at 5 microM). In addition, it delayed LDL oxidation with a prolonged lag time (192 min at 2 microM, control: 44 min). This compound also showed a 10-fold more potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC50: 1.7 microM) than trolox (IC50: 18.6 microM), a known DPPH inhibitor. In addition, phellinsin A inhibited xanthine oxidase activity with an IC50 value of 31.0 microM, whereas allopurinol, a xanthine oxidase inhibitor, showed an IC50 value of 40.7 microM.
Hwang
Eui Il
EI
Agro-Tech Research Group, KT&G Central Research Institute, Daejeon 305-805, Korea.
Kim
Ju-Ryoung
JR
Jeong
Tae-Sook
TS
Lee
Sangku
S
Rho
Mun-Chual
MC
Kim
Sung Uk
SU
D000363:Agaricales; D000924:Anticholesteremic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001713:Biphenyl Compounds; D008078:Cholesterol, LDL / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D007783:Lactones / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry
D000924:Anticholesteremic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D008078:Cholesterol, LDL; D007783:Lactones; C417111:Phellinsin A; D010636:Phenols; D010851:Picrates; D017392:Thiobarbituric Acid Reactive Substances; C004931:1,1-diphenyl-2-picrylhydrazyl