Peripheral blood lymphocytes P-glycoprotein (P-gp, gp-170) expression in allogenic kidney transplant patients.
OBJECTIVE
P-glycoprotein (gp-170, P-gp) is a transmembrane transporter involved in drug, for example cyclosporine A, efflux from the cells thus limiting their intracellular concentration. Expression of the transporter on the surface of immune competent cells may be associated with poor prognosis in kidney transplant patients. The aim of the present study was to evaluate P-gp expression on the surface of CD4(+), CD8(+), CD19(+) and CD56(+) cells in kidney transplant patients treated with cyclosporine A as a main immunosuppressant, using flow cytometry.


RESULTS
It was found that P-gp expression in kidney transplant patients with acute rejection did not differ significantly from transplanted patients without rejection studied in the same period after transplantation, as well as from the healthy controls. Administration of 3-day course of 1,000 mg/24 h methylprednisolone did not affect the expression of P-gp in the studied cells, except for significant elevation in CD56(+) cells, which disappeared at 2 weeks after cessation of steroid administration.


CONCLUSIONS
Based on the results from the present study it can be concluded that P-gp expression is not a prognostic factor of acute kidney graft rejection.
Kotrych
Katarzyna
K
Department of Pharmacology, Pomeranian Medical University, Szczecin, Poland.
Masiuk
Marek
M
Domański
Leszek
L
Rózański
Jacek
J
Droździk
Marek
M
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000208:Acute Disease; D000328:Adult; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D013601:T-Lymphocytes / Q000378:metabolism*; D014184:Transplantation, Homologous / Q000276:immunology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1