title: Concentration of soluble P-selectin and white blood cell counts in infarct coronary arteries in patients with acute myocardial infarction differ from the systemic circulation.
abstract: BACKGROUND
  Previously, researchers have suggested that Soluble (s) P-selectin mediates the accumulation of leukocytes which in turn promotes fibril deposition. Soluble P-selectin and white blood cell (WBC) counts have been shown to be increased in the systemic circulation after acute myocardial infarction (AMI). However, whether the infarct coronary artery (ICA) and systemic circulation differs with respect to the concentration of sP-selectin and WBC counts following AMI remain unknown. In this study, we investigated whether the concentration of sP-selectin and WBC counts differed between the ICA and the systemic circulation after AMI.
  
  
  METHODS
  Blood samples for circulating sP-selectin and WBC counts were immediately obtained after vascular puncture in 72 patients with AMI of < 12 h undergoing primary percutaneous coronary intervention (PCI). Additionally, blood samples for ICA sP-selectin and WBC counts were obtained via Export Suction Catheter during PCI. For comparison, blood samples for sP-selectin and WBC counts were obtained once in 30 healthy subjects.
  
  
  RESULTS
  The results demonstrated that the circulating sP-selectin [64.7 +/- 18.1 (ng/ml) vs. 29.5 +/- 6.3 (ng/ml), p < 0.0001] and WBC counts [12.1 +/- 3.6 (x 10(3)/ml) vs. 5.0 +/- 1.0 (x 10(3)/ml), p < 0.0001] were significantly higher in our patients than in healthy subjects. Furthermore, the sP-selectin [72.7 +/- 23.3 (ng/ml) vs. 64.7 +/- 18.1 (ng/ml), p < 0.0001] and the WBC counts [16.2 +/- 3.8 (x 10(3/)ml) vs. 12.1 +/- 3.6 (x 10(3)/ml), p < 0.0001] were markedly higher in the ICA than in the systemic circulation for the patients.
  
  
  CONCLUSIONS
  The plasma level of sP-selectin and WBC counts were more elevated in the ICA than in the systemic circulation of patients with AMI undergoing primary PCI. These findings strengthen the role of sequestration of WBC and sP-selectin in the ICA as crucial in thrombus formation.
authors.affiliation: Division of Cardiology, Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
authors.forename: Teng-Hung
authors.initials: TH
authors.lastname: Yu
authors.forename: Sarah
authors.initials: S
authors.lastname: Chua
authors.forename: Cheng-I
authors.initials: CI
authors.lastname: Cheng
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Liu
authors.forename: Cheng-An
authors.initials: CA
authors.lastname: Chiu
authors.forename: Cheng-Hsu
authors.initials: CH
authors.lastname: Yang
authors.forename: Chih-Yuan
authors.initials: CY
authors.lastname: Fang
authors.forename: Yuan-Kai
authors.initials: YK
authors.lastname: Hsieh
authors.forename: Chi-Ling
authors.initials: CL
authors.lastname: Hang
authors.forename: Wei-Chin
authors.initials: WC
authors.lastname: Hung
authors.forename: Yen-Hsun
authors.initials: YH
authors.lastname: Chen
authors.forename: Kuo-Ho
authors.initials: KH
authors.lastname: Yeh
authors.forename: Morgan
authors.initials: M
authors.lastname: Fu
authors.forename: Hon-Kan
authors.initials: HK
authors.lastname: Yip
mesh_terms: D000328:Adult; D000368:Aged; D015906:Angioplasty, Balloon, Coronary; D003331:Coronary Vessels / Q000737:chemistry*; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000628:therapy; D019007:P-Selectin / Q000097:blood*
chemical_list: D019007:P-Selectin