Direct confinement of individual viruses within polyethylene glycol (PEG) nanowells.
Individual M13 viruses were spatially confined within wells fabricated from nanomolding of a PEG-based random copolymer. The viruses were selectively adhered to the region pretreated with an antibody against the virus, resulting in individual virus arrays. The polymer surface was found to be highly resistant to the attachment of the virus (approximately 0.02 microm-2), approximately 2 orders of magnitude lower than that on a bare silicon surface. The physical height of the template provided an additional barrier to the attachment of the virus due to entropic penalty in bending of a semi-flexible M13 virus. The effects of pattern size and barrier height were investigated, revealing that a certain critical height is needed to ensure successful confinement within the template for a given pattern size.
Suh
Kahp Y
KY
School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, Korea, and Harvard-MIT Division of Health Sciences and Technology, Brigham and Women's Hospital, Cambridge, Massachusetts 02139, USA.
Khademhosseini
Ali
A
Jon
Sangyong
S
Langer
Robert
R
D017104:Bacteriophage M13 / Q000737:chemistry*; D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003460:Crystallization / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
D011092:Polyethylene Glycols