Antioxidant constituents in the fruits of Luffa cylindrica (L.) Roem.
Hydrophilic antioxidant constituents in the fruits of the vegetable Luffa cylindrica (L.) Roem (sponge gourds) were separated by an antioxidant-guided assay to yield eight compounds: p-coumaric acid (1), 1-O-feruloyl-beta-D-glucose (2), 1-O-p-coumaroyl-beta-D-glucose (3), 1-O-caffeoyl-beta-D-glucose (4), 1-O-(4-hydroxybenzoyl)glucose (5), diosmetin-7-O-beta-D-glucuronide methyl ester (6), apigenin-7-O-beta-D-glucuronide methyl ester (7), and luteolin-7-O-beta-D-glucuronide methyl ester (8). The eight compounds were isolated by high-speed countercurrent chromatography and identified by electrospray ionization-mass spectrometry and NMR analysis, and the antioxidant activity was evaluated by the radical scavenging effect on the 1,1-diphenyl-2-picrylhydrazyl radical. High-performance liquid chromatography analysis showed that a total amount of the eight compounds in the dried gourds without skin was about 1%. The results demonstrate that the consumption of sponge gourds can supply some antioxidant constituents to human body.
Du
Qizhen
Q
Institute of Food and Biological Engineering, Zhejiang Gongshang University, 149 Jiaogong Road, Hangzhou 310035, China. qizhendu@163.com
Xu
Yuanjin
Y
Li
Lei
L
Zhao
Yang
Y
Jerz
Gerold
G
Winterhalter
Peter
P
D000975:Antioxidants / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D029776:Luffa / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization
D000975:Antioxidants; D010636:Phenols