Rabies virus binding to cellular membranes measured by enzyme immunoassay.
The binding of rabies virus to cellular membranes was measured using an enzyme-linked immunosorbent assay (ELISA). Virus binding to membranes adsorbed to the wells of microtiter plates was detected with rabies virus antibody and alkaline phosphatase-linked second antibody. The greatest degree of binding was to myotube, neuroblastoma, and salivary gland membranes; intermediate levels occurred in striated muscle and nerve membranes; and low levels of binding were found in other membranes, including those of most parenchymal organs. Binding of rabies virus to myotube membranes was saturable, dependent on pH (with an optimum of pH 6.0), facilitated by the divalent cations Ca++, Mn++, and Mg++, and was temperature dependent. Binding was greatly reduced by inactivation of virus with beta-propiolactone or treatment of virus with trypsin. In embryonic chick myotubes, total acetylcholine receptor content and acetylcholinesterase activity undergo marked changes during development, first increasing and then decreasing at the time of hatching. Binding of rabies virus followed a similar pattern, indicating that the virus may interact with the acetylcholine receptor or other surface molecules undergoing similar developmental changes.
Lentz
T L
TL
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA.
Chester
J
J
Benson
R J
RJ
Hawrot
E
E
Tignor
G H
GH
Smith
A L
AL
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001666:Binding Sites, Antibody / Q000187:drug effects / Q000502:physiology; D001667:Binding, Competitive / Q000187:drug effects / Q000502:physiology; D002412:Cations / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism* / Q000821:virology*; D002642:Chick Embryo; D006224:Cricetinae; D004285:Dogs; D004622:Embryo, Mammalian; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006168:Guinea Pigs; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000187:drug effects; D009447:Neuroblastoma; D011420:Propiolactone / Q000494:pharmacology; D011820:Rabies virus / Q000276:immunology / Q000502:physiology*; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology
D000906:Antibodies; D002412:Cations; D011950:Receptors, Cholinergic; D011420:Propiolactone; D000110:Acetylcholinesterase; D014357:Trypsin