Antigen-specific expansion of TCR Vbeta3+ CD4+ T cells in the early stage of collagen-induced arthritis and its arthritogenic role in DBA/1J mice.
To investigate type II collagen (CII)-specific CD4+ T cell receptors involving in Collagen-induced arthritis (CIA) in DBA/1J mice as a model of rheumatoid arthritis in humans, TCR Vbeta usage in draining lymph nodes (dLNs) was assessed by flow cytometric analysis at 3, 5, and 8 weeks after bovine CII immunizations. In the early stage of CIA, the draining lymph node CD4+ T cells from CIA mice showed a higher proportion of CD4+ Vbeta3+ subsets compared with those from control mice. The CD4+ Vbeta3+ T cells were specifically and primarily expanded by antigen-specific stimulation in in vitro culture of dLNs lymphocytes and splenocytes from CIA mice. In addition, CII-reactive response was observed when CD4+ Vbeta3+ T cells were added to a non-responding T cell population. The adoptive transfer of CD4+ Vbeta3+ T cells produced exaggerated arthritis compared with that in the control group. Our results indicate that CD4+ Vbeta3+ T cells, which were selectively expanded in dLN of CIA mice, play a pivotal role in CIA pathogenesis.
Lee
Jae-Seon
JS
Department of Medicine, Division of Rheumatology, Center for Rheumatoid Diseases, Catholic Research Institutes of Medical Sciences, Catholic University of Korea, Seoul, Republic of Korea.
Cho
Mi-La
ML
Jhun
Joo-Yeon
JY
Min
So-Youn
SY
Ju
Ji-Hyeon
JH
Yoon
Chong-Hyeon
CH
Min
Jun-Ki
JK
Park
Sung-Hwan
SH
Kim
Ho-Youn
HY
Cho
Young-Gyu
YG
D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D024043:Collagen Type II / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000473:pathology
D024043:Collagen Type II; D016693:Receptors, Antigen, T-Cell, alpha-beta