title: Crystallization and preliminary X-ray diffraction analysis of a tRNASer acceptor-stem microhelix.
abstract: In order to understand elongator tRNA(Ser) and suppressor tRNA(Sec) identity elements, the respective acceptor-stem helices have been synthesized and crystallized in order to analyse and compare their structures in detail at high resolution. The synthesis, crystallization and preliminary X-ray diffraction results for a seven-base-pair tRNA(Ser) acceptor-stem helix are presented here. Diffraction data were collected to 1.8 A, applying synchrotron radiation and cryogenic cooling. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 36.14, b = 38.96, c = 30.81 A, beta = 110.69 degrees .
authors.affiliation: Institute of Chemistry and Biochemistry, Free University Berlin, Thielallee 63, 14195 Berlin, Germany.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Förster
authors.forename: Norbert
authors.initials: N
authors.lastname: Krauss
authors.forename: Arnd B E
authors.initials: AB
authors.lastname: Brauer
authors.forename: Karol
authors.initials: K
authors.lastname: Szkaradkiewicz
authors.forename: Svenja
authors.initials: S
authors.lastname: Brode
authors.forename: Klaus
authors.initials: K
authors.lastname: Hennig
authors.forename: Jens P
authors.initials: JP
authors.lastname: Fürste
authors.forename: Markus
authors.initials: M
authors.lastname: Perbandt
authors.forename: Christian
authors.initials: C
authors.lastname: Betzel
authors.forename: Volker A
authors.initials: VA
authors.lastname: Erdmann
mesh_terms: D001483:Base Sequence; D003460:Crystallization; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D012362:RNA, Transfer, Ser / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D009841:Oligonucleotides; D012362:RNA, Transfer, Ser