[Effects of ERK5 MAPK signaling transduction pathway on the inhibition of genistein to breast cancer cells].
OBJECTIVE
To study the role of ERK5 MAPK signaling transduction pathway in the inhibition of genistein to MDA-MB-231 breast cancer cells.


METHODS
The inhibitive effects of genistein on MDA-MB-231 cells were observed with the MTT assay. The cell apoptosis was detected by flow cytometry. The ERK5 MAPK, Bax and Caspase3 protein expressions were detected by using western blot method.


RESULTS
genistein inhibited the cell proliferation of MDA-MB-231 cells and induced apoptosis. The protein expression of ERK5 decreased and those of Bax and Caspase3 increased with the dose of genistein.


CONCLUSIONS
Genistein can affect the ERK5 MAPK signaling transduction pathway and induce the expressions of apoptosis related proteins to inhibit the proliferation of MDA-MB-231 cells.
Li
Jing
J
Department of Nutrition and Food Hygiene, Nanjing Medical University, Nanjing 210029, China.
Li
Zhong
Z
Mo
Bao-qing
BQ
D016588:Anticarcinogenic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology* / Q000517:prevention & control; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D048671:MAP Kinase Kinase 5 / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
D016588:Anticarcinogenic Agents; D019833:Genistein; D048671:MAP Kinase Kinase 5