title: Thyroid peroxidase immunohistochemistry in differential diagnosis of thyroid tumors.
abstract: Thyroperoxidase (TPO) is a thyroid-specific enzyme expressed by differentiated thyroid cells. Initial immunohistochemical studies claimed that TPO expression, detected by the monoclonal antibody mAb 47, may be a potentially important diagnostic tool in differentiating malignant from benign lesions. However, some recent studies have failed to reproduce the earlier results, suggesting the limitations for TPO immunohistochemistry. To assess these observations we have evaluated the immunohistochemical expression of TPO in thyroid tissue from 215 patients. The studied material included 87 nonmalignant thyroid lesions and 128 thyroid carcinomas. TPO expression was investigated using newly available mAb 47 and staining of less than 80% of the follicular cells/specimen as the threshold indicating a malignant lesion. We found that TPO had a sensitivity of 89.9% for cancer and a specificity of 64.4% for nonmalignant lesions, showing that it does not give a sufficient degree of diagnostic certainty that the lesion is benign. In addition, the variability in the degree of TPO expression found within and between follicular carcinomas, and the significant number of benign adenomas having similar immunostaining patterns, assured us that TPO immunostaining is not sufficiently discriminatory in the differential diagnosis of thyroid cancer versus benign lesions.
authors.affiliation: Institute for the Application of Nuclear Energy-INEP, University of Belgrade, Zemun, Belgrade. ssavin@inep.co.yu
authors.forename: Svetlana
authors.initials: S
authors.lastname: Savin
authors.forename: Dubravka
authors.initials: D
authors.lastname: Cvejic
authors.forename: Tijana
authors.initials: T
authors.lastname: Isic
authors.forename: Ivana
authors.initials: I
authors.lastname: Petrovic
authors.forename: Ivan
authors.initials: I
authors.lastname: Paunovic
authors.forename: Svetislav
authors.initials: S
authors.lastname: Tatic
authors.forename: Marija
authors.initials: M
authors.lastname: Havelka
mesh_terms: D000236:Adenoma / Q000201:enzymology* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism; D002277:Carcinoma / Q000201:enzymology* / Q000473:pathology; D003937:Diagnosis, Differential; D007124:Immunoenzyme Techniques; D007453:Iodide Peroxidase / Q000378:metabolism*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013959:Thyroid Diseases / Q000175:diagnosis / Q000201:enzymology; D013964:Thyroid Neoplasms / Q000201:enzymology* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D007453:Iodide Peroxidase