Deglycosylation of CD147 down-regulates Matrix Metalloproteinase-11 expression and the adhesive capability of murine hepatocarcinoma cell HcaF in vitro.
CD147 is a plasma membrane glycoprotein, enriched on the surface of many malignant tumor cells. As a result of heterogeneous N-glycosylation, CD147 exists in both a highly glycosylated form, HG-CD147 ( approximately 40-60 kDa) and lowly glycosylated form, LG-CD147 ( approximately 32 kDa). This experiment investigated the possible role of CD147 glycosylation in the HcaF, HcaP and Hepa1-6 mouse hepatocarcinoma cell lines, which have high, low and no metastatic potential in the lymph nodes. Western blot analysis showed that the ratio of HG-CD147/LG-CD147 protein expression on HcaF and HcaP were much higher than that on Hepa1-6 cells. By treatment with tunicamycin (TM), an inhibitor of N-glycosylation, the expression level of HG-CD147 decreased and the LG-CD147 disappeared completely in HcaF cells. Meanwhile, Matrixmetallproteinase-11 (MMP-11) protein expression was down-regulated, and the adhesive capability of HcaF cells to endothelial cells in cryosection of mouse lymph nodes decreased. These results indicated that the glycosylation of CD147 plays a crucial role. It is HG-CD147 that may contribute more to tumor progress, invasion and metastasis into lymph node rather than LG-CD147. The results of this study are of biological and clinical importance.
Jia
Li
L
Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian, China.
Zhou
Huimin
H
Wang
Shujing
S
Cao
Jun
J
Wei
Wei
W
Zhang
Jianing
J
D000818:Animals; D051926:Basigin / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000503:physiopathology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology; D006031:Glycosylation; D008113:Liver Neoplasms / Q000201:enzymology / Q000503:physiopathology*; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases / Q000096:biosynthesis*; D051379:Mice; D014415:Tunicamycin / Q000494:pharmacology
C497887:Bsg protein, mouse; D014415:Tunicamycin; D051926:Basigin; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases