[Establishment of whole pancreaticoduodenal allotransplantation model with portal venous drainage and enteric drainage in pigs].
OBJECTIVE
To establish an porcine model of whole pancreaticoduodenal transplantation with portal venous drainage and enteric drainage for ensuring physiologically normal function without hyperinsulinemia and reducing postoperative complications.


METHODS
Twenty sichuan native outbreding white pigs weighing 25-30 kg were divided equally into two groups to serve as the donors and recipients. Cooling of the grafts was accomplished with in situ flush with 4 degrees C UW preservation solution via an aortic cannula. A whole pancreatoduodenal graft with the segment of abdominal aorta and the portal vein was harvested from the donor pigs. Type I diabetes model was established by complete removal of the recipient pancreas. The whole pancreatoduodenal graft was preserved and shaped in UW solution, and the subphrenic abdominal aorta of the recipient was joined with the donor abdominal aorta via a side-to-end anastomosis, and venous reflux was reconstructed between the donor portal vein and the recipient superior mesenteric vein. Side-to-side intestinal anastomosis was performed between the donor duodenum and the recipient jejunum.


RESULTS
Ten pancreaticoduodenal transplantations (PVE+ED style) were done, and pancreatic graft thrombosis and embolism occurred only in 1 pig 6 days after transplantation.


CONCLUSIONS
The model of whole pancreaticoduodenal transplantation with portal venous drainage and enteric drainage is stable and reliable.
Dong
Gao-hong
GH
Center of Organ Transplantation, First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
Zhang
Zhao-da
ZD
Hu
Wei-ming
WM
Tang
Yong
Y
Li
Jian-shui
JS
D000818:Animals; D004322:Drainage / Q000379:methods*; D004386:Duodenum / Q000637:transplantation*; D005260:Female; D007422:Intestines / Q000601:surgery; D008297:Male; D023421:Models, Animal; D016035:Pancreas Transplantation; D011169:Portal Vein / Q000601:surgery; D013552:Swine; D014184:Transplantation, Homologous