title: Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes.
abstract: Bidens pilosa (B. pilosa) is well known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of both the ethanol (EtOH) and ethylacetate/ethanol (EA/EtOH) extracts from the whole B. pilosa plant, to protect normal human erythrocytes against oxidative damage in vitro. It was determined that the oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by the aqueous peroxyl radical [2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)] were suppressed by both EtOH (50-150 microg/ml) and EA/EtOH (25-75 microg/ml) extracts of B. pilosa in concentration- and time-dependent manners. B. pilosa extracts also prevented the decline of superoxide dismutase (SOD) activity and the depletion of cytosolic glutathione (GSH) and ATP in erythrocytes. These results imply that B. pilosa may have protective antioxidant properties.
authors.affiliation: Institute of Nutrition, China Medical University, Taichung, Taiwan.
authors.forename: Hsin-Ling
authors.initials: HL
authors.lastname: Yang
authors.forename: Ssu-Ching
authors.initials: SC
authors.lastname: Chen
authors.forename: Nai-Wen
authors.initials: NW
authors.lastname: Chang
authors.forename: Jia-Ming
authors.initials: JM
authors.lastname: Chang
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Lee
authors.forename: Pei-Chuan
authors.initials: PC
authors.lastname: Tsai
authors.forename: Han-Hsuan
authors.initials: HH
authors.lastname: Fu
authors.forename: Wei-Wan
authors.initials: WW
authors.lastname: Kao
authors.forename: Hsiao-Chi
authors.initials: HC
authors.lastname: Chiang
authors.forename: Hsuan-Hui
authors.initials: HH
authors.lastname: Wang
authors.forename: You-Cheng
authors.initials: YC
authors.lastname: Hseu
mesh_terms: D000085:Acetates / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology*; D031176:Bidens / Q000737:chemistry; D002478:Cells, Cultured; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000085:Acetates; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D008565:Membrane Proteins; D000431:Ethanol; C007650:ethyl acetate; D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase; D005978:Glutathione