title: Neural nitric oxide gene inactivation affects the release profile of oxytocin into the blood in response to forced swimming.
abstract: This study was undertaken to examine the importance of nitric oxide (NO) generated by the neural isoform of the nitric oxide synthase (nNOS) on the activity of the hypothalamic neurohypophyseal system in neural nitric oxide synthase knock-out (KO) and wild-type (WT) mice under basal conditions and in response to forced swimming. The intensity of the hybridisation signal for vasopressin (AVP) in the hypothalamic supraoptic nucleus (SON) was significantly higher in KO mice when compared with WT, whereas oxytocin (OXT) basal mRNA levels were similar in both groups. Although the basal peripheral release of AVP and OXT was equivalent in both genotypes, we observed in KO mice a significant drop of AVP and OXT plasma values 15 min after stressor onset and a robust increase in the OXT plasma concentration at 60 min. These findings suggest that in the male mouse, NO inhibits AVP gene transcription in magnocellular neurones of the SON and collaborates in maintaining constant AVP and OXT plasma levels following acute stressor exposure, exerting a bimodal regulatory action on OXT secretion. We conclude that NO is involved in the regulation of magnocellular neurones of the SON, and it is preferentially implicated in the attenuation of the peripheral release of OXT induced by acute stressor exposure.
authors.affiliation: Institute of Medical Neurobiology, Otto-von-Guericke University, Leipziger Str. 44, D-39120 Magdeburg, Germany. gabriella.orlando@medizin.uni-magdeburg.de
authors.forename: G F
authors.initials: GF
authors.lastname: Orlando
authors.forename: K
authors.initials: K
authors.lastname: Langnaese
authors.forename: R
authors.initials: R
authors.lastname: Landgraf
authors.forename: M G
authors.initials: MG
authors.lastname: Spina
authors.forename: G
authors.initials: G
authors.lastname: Wolf
authors.forename: M
authors.initials: M
authors.lastname: Engelmann
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D020868:Gene Silencing; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D052248:Nitric Oxide Synthase Type I / Q000235:genetics* / Q000502:physiology; D010121:Oxytocin / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D013550:Swimming
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D001127:Arginine Vasopressin; D010121:Oxytocin; D052248:Nitric Oxide Synthase Type I