[Research on the expression of cancer associated gene in the intracranial tumors].
OBJECTIVE
To investigate the function significance and application value of cancer associated gene (CAGE) in the pathogenesis of intracranial tumors.


METHODS
Semi-quantitative RT-PCR was performed to detect the expression of CAGE gene in 17 normal tissues, 35 meningioma cases and 63 glioma cases.


RESULTS
The CAGE gene was expressing in none of the normal tissues except testis, but in 4 meningioma (11.3%) and 57 glioma cases(90.5%). The expression of CAGE gene showed significant difference between the meningioma and glioma (P<0.001). The amount of CAGE mRNA was 0.5 +/- 0.11, 0.86 +/- 0.23, 1.85 +/- 0.37 or 2.7 +/- 0.46 times of the internal control one in glioma grade I, II, or IV respectively. The expression of CAGE gene increased with the pathological grade(r=0.82).


CONCLUSIONS
The CAGE plays important role in the pathogenesis of glioma, and may act as the tumor specific antigen for glioma.
Gao
Fei
F
Department of Medical Genetics, China Medical University, Shenyang 110001, China.
You
Tian
T
Lü
Jing-yu
JY
Wang
Yun-jie
YJ
D000328:Adult; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008579:Meningioma / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged
D000951:Antigens, Neoplasm; C454199:DDX53 protein, human; D053487:DEAD-box RNA Helicases