title: Participation of ATP/ADP antiporter in oleate- and oleate hydroperoxide-induced uncoupling suppressed by GDP and carboxyatractylate.
abstract: In experiments on isolated kidney and liver mitochondria, it is shown that oleate hydroperoxide induces a much smaller increase in the controlled respiration rate and DeltaPsi decrease than the same concentrations of oleate. Palmitate appears to be less efficient than oleate but more efficient than oleate hydroperoxide. In all cases, GDP and CAtr cause some recoupling, CAtr being more effective. Addition of 0.2 mM GDP before CAtr does not prevent further DeltaPsi increase by subsequent CAtr addition. On the other hand, GDP added after CAtr is without any effect. GDP partially prevents the DeltaPsi lowering by ADP at the State 4--State 3 transition if small amounts of CAtr are present. The data are consistent with the suggestion of F. Goglia and V.P. Skulachev (FASEB J. 17, 1585-1591, 2003) that fatty acid anions are translocated by mitochondrial anion carriers much better than their hydroperoxides. As to GDP recoupling, it cannot be regarded as a specific probe for uncoupling by UCPs since it can be mediated by the ATP/ADP antiporter.
authors.affiliation: Department of Bioenergetics, A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119992, Russia.
authors.forename: Lyudmila S
authors.initials: LS
authors.lastname: Khailova
authors.forename: Elena A
authors.initials: EA
authors.lastname: Prikhodko
authors.forename: Vera I
authors.initials: VI
authors.lastname: Dedukhova
authors.forename: Elena N
authors.initials: EN
authors.lastname: Mokhova
authors.forename: Vasily N
authors.initials: VN
authors.lastname: Popov
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Skulachev
mesh_terms: D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives* / Q000494:pharmacology; D019069:Cell Respiration / Q000187:drug effects; D006153:Guanosine Diphosphate / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007473:Ion Channels / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism; D019301:Oleic Acid / Q000494:pharmacology*; D051381:Rats; D014475:Uncoupling Agents / Q000494:pharmacology; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D014475:Uncoupling Agents; D000071256:Uncoupling Protein 1; D006153:Guanosine Diphosphate; D001278:Atractyloside; D019301:Oleic Acid; D000226:Mitochondrial ADP, ATP Translocases; D006861:Hydrogen Peroxide; C003853:carboxyatractyloside