title: Molecular cloning of bovine chemokine receptors and expression by WC1+ gammadelta T cells.
abstract: Chemokine receptors mediate leukocyte migration into secondary lymphoid tissues and localization to peripheral inflammation sites. We describe full-length cDNA sequences of bovine chemokine receptors CCR5, CCR7, CXCR3 and CXCR5 and transcript expression by WC1(+)gammadelta T cells, a unique cell population with proinflammatory characteristics that comprises a large proportion of mononuclear cells in young ruminants. Bovine chemokine sequences were more similar to those of humans than were murine sequences to humans', ranging from 84% to 91%. Transcript analysis showed that antigen stimulation of WC1(+)gammadelta T cells induced IFN-gamma production and substantially increased CCR5 and CXCR3 expression when compared with freshly isolated (ex vivo) cells. CCR7 transcripts were minimally expressed in ex vivo and proliferating WC1(+)gammadelta T cells and CXCR5 expression was negligible. These results confirm the proinflammatory nature of WC1(+)gammadelta T cells is reflected by its chemokine receptor expression and suggest WC1(+)gammadelta T cells are unlikely to transit through secondary lymphoid tissues.
authors.affiliation: Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA.
authors.forename: Seth L
authors.initials: SL
authors.lastname: Blumerman
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Carolyn T A
authors.initials: CT
authors.lastname: Herzig
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Baldwin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D007249:Inflammation; D007371:Interferon-gamma / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000276:immunology; D008969:Molecular Sequence Data; D019707:Receptors, Chemokine / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism
chemical_list: C086500:DMBT1 protein, Bos taurus; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D019707:Receptors, Chemokine; D007371:Interferon-gamma