title: Regulatory variability of camalexin biosynthesis.
abstract: The anthranilate synthase ASA1, CYP79B2 and CYP71B15 (PAD3) are biosynthetic genes of the Arabidopsis phytoalexin camalexin, which are induced after pathogen infection and abiotic treatments like silver nitrate spraying. The natural variation of camalexin biosynthesis in response to Pseudomonas syringae infection was determined in several ecotypes, and differential CYP71B15 regulation as a potential basis for this variation was investigated. The expression of camalexin biosynthetic genes was restricted to the tissue undergoing cell death. After droplet infection with Alternaria alternata, a potent camalexin inducer in the Col-0 ecotype, camalexin formation and the induction of ASA1, CYP79B2 and CYP71B15 were strictly co-localized with the infection site.
authors.affiliation: Lehrstuhl für Genetik, Technische Universität München, Am Hochanger 8, D-85350 Freising, Germany.
authors.forename: Regina
authors.initials: R
authors.lastname: Schuhegger
authors.forename: Thomas
authors.initials: T
authors.lastname: Rauhut
authors.forename: Erich
authors.initials: E
authors.lastname: Glawischnig
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007211:Indoles / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D044224:Pseudomonas syringae; D012835:Silver Nitrate / Q000494:pharmacology; D013844:Thiazoles / Q000378:metabolism*; D014158:Transcription, Genetic; D000094162:Phytoalexins
chemical_list: D029681:Arabidopsis Proteins; D007211:Indoles; D012835:Silver Nitrate; D013844:Thiazoles; C102405:camalexin; D000094162:Phytoalexins