Specificity and expression of RalGPS as RalGEFs.
Ral proteins regulate a variety of biological processes and are major downstream targets of Ras because of association of the RalGDS family of exchange factors with Ras-GTP. However, a second, less-characterized family of Ral GEFs has also been isolated. Described here are methods used to determine the substrate specificity of this RalGPS family of GEFs, measure their membrane localization, interactions of with SH3 domains and phospholipids, as well as to measure their ability to activate Ral in vivo.
Quilliam
Lawrence A
LA
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine and Walther Cancer Institute, Indianapolis, Indiana, USA.
D002458:Cell Fractionation; D002460:Cell Line; D006801:Humans; D010743:Phospholipids / Q000378:metabolism; D013379:Substrate Specificity; D014162:Transfection; D020726:ral Guanine Nucleotide Exchange Factor / Q000096:biosynthesis* / Q000378:metabolism; D018909:src Homology Domains / Q000502:physiology
D010743:Phospholipids; D020726:ral Guanine Nucleotide Exchange Factor