The effect of salidroside on cell damage induced by glutamate and intracellular free calcium in PC12 cells.
Salidroside (Sald), was extracted from Rhodiola rosea L, a traditional Chinese medicine which has been used for long time for anti-aging, anti-cancer and anti-oxidative stress etc. In present experiment, salidroside could protect the PC12 cell against injuries caused by exposure of PC12 cells to 2 mmol/L glutamate for 15 min followed by incubation with serum-free medium for 24 h, which resembled the excitotoxin in vivo system. Furthermore, saldroside could decrease the [Ca2+]i of PC12 cells in Mg2+-free Hanks' solution and D-Hanks' solution but there was no effect on basal [Ca2+]i in Hanks' solution. The studies also indicated that salidroside inhibited the increases of [Ca2+]i induced by KCl and glutamate. In conclusion, salidroside may protect PC12 cell against glutamate excitotoxic damage through suppressing the excessive entry of Ca2+ and the release of the calcium stores.
Cao
Li-Li
LL
Shenyang Pharmaceutical University, Shenyang 110016, China.
Du
Guan-Hua
GH
Wang
Min-Wei
MW
D000311:Adrenal Glands / Q000166:cytology / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D018698:Glutamic Acid / Q000633:toxicity*; D007424:Intracellular Fluid / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D016716:PC12 Cells; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D011189:Potassium Chloride; D051381:Rats
D005960:Glucosides; D010636:Phenols; D018698:Glutamic Acid; D011189:Potassium Chloride; C009172:rhodioloside; D002118:Calcium