title: Antioxidant and anti-inflammatory activities of the methanolic extract of Neorhodomela aculeate in hippocampal and microglial cells.
abstract: The antioxidant and anti-inflammatory properties of the marine red alga Neorhodomela aculeate (N. aculeata) Masuda were investigated with neuronal and microglial cells. Extracts of N. aculeata had potent neuroprotective effects on glutamate-induced neurotoxicity and inhibited reactive oxygen species (ROS) generation in the murine hippocampal HT22 cell line. Also, extracts of N. aculeata inhibited H2O2-induced lipid peroxidation in rat brain homogenates. The properties of the extract as an anti-inflammatory agent were investigated in microglial activation by interferon-gamma (IFN-gamma): it reduced the inducible nitric oxide synthase that consequently resulted in the reduction of nitric oxide. These results suggest that the marine red alga N. aculeata could be considered as a potential source for reducing reactive oxygen species and inflammation related to neurological diseases.
authors.affiliation: Graduate Program in Neuroscience, Institute for Brain Science and Technology (IBST), Inje University, Daejeon, South Korea.
authors.forename: Chol Seung
authors.initials: CS
authors.lastname: Lim
authors.forename: Da-Qing
authors.initials: DQ
authors.lastname: Jin
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Sung
authors.forename: Ji Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Han Gil
authors.initials: HG
authors.lastname: Choi
authors.forename: Ilho
authors.initials: I
authors.lastname: Ha
authors.forename: Jung-Soo
authors.initials: JS
authors.lastname: Han
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D000432:Methanol / Q000737:chemistry; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010851:Picrates / Q000737:chemistry; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D000461:Rhodophyta / Q000737:chemistry*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D001713:Biphenyl Compounds; D018696:Neuroprotective Agents; D010851:Picrates; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol