title: SFTG international collaborative study on in vitro micronucleus test IV. Using CHL cells.
abstract: In this report, are presented the results of an international collaborative study on the in vitro micronucleus assay, using CHL cells. Fourteen laboratories participated in this study which was coordinated by an organizing committee supported by the SFTG (the French branch of the European Environmental Mutagen Society). Nine coded substances, having different modes of action and at different levels were assessed in the in vitro micronucleus test, using a common protocol. Mitomycin C was used as a positive control. In order to help to define a standard protocol on CHL cells, short and long treatment periods followed by various recovery times, with or without cytochalasin B, were compared. After an evaluation of the acceptability of the assays, the tested chemicals were classified as negative, positive or equivocal. Mannitol and clofibrate were judged as negative in all treatment schedules. Bleomycin was positive in all the treatment schedules, with an increase in the number of micronucleated cells in both mononucleate and binucleate cells when using cytochalasin B. This was also shown for the aneugens colchicine, diethylstilboestrol and griseofulvin, as expected. Urethane was judged as equivocal only after long treatment with cytochalasin B, and negative in all other treatment schedules. In any case, no genotoxic compound would have been missed with schedules including a short and a long treatment time, whether the treatment was followed by a recovery period or not and whether cytochalasin B was used or not. Thus, these results show that CHL cells were suitable for accurately detecting clastogenic and aneugenic compounds of various types in the in vitro micronucleus test.
authors.affiliation: Astellas Pharma Inc., Drug Safety Research Laboratories, Osaka, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Wakata
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsuoka
authors.forename: Kohji
authors.initials: K
authors.lastname: Yamakage
authors.forename: Junichi
authors.initials: J
authors.lastname: Yoshida
authors.forename: Kinya
authors.initials: K
authors.lastname: Kubo
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Nami
authors.initials: N
authors.lastname: Senjyu
authors.forename: Satoru
authors.initials: S
authors.lastname: Itoh
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Miyajima
authors.forename: Shuichi
authors.initials: S
authors.lastname: Hamada
authors.forename: Souji
authors.initials: S
authors.lastname: Nishida
authors.forename: Harumi
authors.initials: H
authors.lastname: Araki
authors.forename: Eiji
authors.initials: E
authors.lastname: Yamamura
authors.forename: Akiko
authors.initials: A
authors.lastname: Matsui
authors.forename: Veronique
authors.initials: V
authors.lastname: Thybaud
authors.forename: Giocondo
authors.initials: G
authors.lastname: Lorenzon
authors.forename: Daniel
authors.initials: D
authors.lastname: Marzin
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Lorge
mesh_terms: D024422:Aneugens / Q000633:toxicity; D000818:Animals; D001761:Bleomycin / Q000633:toxicity; D002460:Cell Line; D002994:Clofibrate / Q000633:toxicity; D003078:Colchicine / Q000633:toxicity; D006224:Cricetinae; D003561:Cytarabine / Q000633:toxicity; D003571:Cytochalasin B; D004054:Diethylstilbestrol / Q000633:toxicity; D005472:Fluorouracil / Q000633:toxicity; D006118:Griseofulvin / Q000633:toxicity; D066298:In Vitro Techniques; D007391:International Cooperation; D008353:Mannitol / Q000633:toxicity; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D014520:Urethane / Q000633:toxicity
chemical_list: D024422:Aneugens; D009153:Mutagens; D003561:Cytarabine; D001761:Bleomycin; D006118:Griseofulvin; D003571:Cytochalasin B; D014520:Urethane; D008353:Mannitol; D004054:Diethylstilbestrol; D002994:Clofibrate; D003078:Colchicine; D005472:Fluorouracil