PON1 55/192 polymorphism, oxidative stress, type, prognosis and severity of stroke.
We investigated the association of PON1 55/192 polymorphisms with type, severity and prognosis of stroke and oxidative markers. Paraoxonase1 (PON1), Glutathione Reductase (GSH-Rd) and Malondialdehyde (MDA) levels were measured at day 1 and at day 5 following the onset of stroke. Genotypes were determined by polymerase chain reaction and restriction digestion. The frequencies of QQ and MM genotypes of PON1 192 and PON1 55, respectively, were significantly higher in controls than in patients. However, the allele frequencies of PON1 192 R and PON1 55 L were significantly more frequent in patients compared to controls. The frequency of combined genotype of RR/LL was significantly higher in cardioembolic group than in atherothrombotic group. PON1 activities were significantly diminished in stroke patients compared to controls. In contrast, serum MDA levels were significantly greater in patients than the values in controls. GSH-Rd activity was higher in patients with small lesion and good prognosis than those with large and poor prognosis. Low density lipoprotein (LDL) levels in patients with large lesions were higher than those with small lesions. PON1 55/192 polymorphisms influence activity of the enzyme. PON1 55/192 genotypes have been associated with MDA levels. In conclusion, PON1 genetic variations are associated with risk factors, severity, type and prognosis of stroke and oxidative stress.
Aydin
Makbule
M
Istanbul University, Institute of Experimental Medicine Research, Department of Neuroscience, Istanbul, Turkey. makbuleaydin@yahoo.com
Gencer
Mehmet
M
Cetinkaya
Yilmaz
Y
Ozkok
Elif
E
Ozbek
Zeynep
Z
Kilic
Gamze
G
Orken
Cihat
C
Tireli
Hulya
H
Kara
Ihsan
I
D000368:Aged; D019943:Amino Acid Substitution / Q000235:genetics; D001120:Arginine / Q000235:genetics; D043303:Aryldialkylphosphatase / Q000235:genetics*; D005260:Female; D005973:Glutamine / Q000235:genetics; D006801:Humans; D007930:Leucine / Q000235:genetics; D008297:Male; D008715:Methionine / Q000235:genetics; D018384:Oxidative Stress / Q000235:genetics* / Q000502:physiology; D011110:Polymorphism, Genetic; D011379:Prognosis; D012307:Risk Factors; D012720:Severity of Illness Index; D020521:Stroke / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism
D005973:Glutamine; D001120:Arginine; D008715:Methionine; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human; D007930:Leucine