title: Differential tissue and subcellular expressionof ERM proteins in normal and malignant tissues: cytoplasmic ezrin expression has prognostic signficance for head and neck squamous cell carcinoma.
abstract: BACKGROUND
  Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC) METHODS: Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue. TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables
  
  
  RESULTS
  ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (>92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p = .04, hazard ratio 1.82)
  
  
  CONCLUSIONS
  Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome.
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, Montefiore Medical Center, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
authors.forename: Rashna
authors.initials: R
authors.lastname: Madan
authors.forename: Margaret
authors.initials: M
authors.lastname: Brandwein-Gensler
authors.forename: Nicolas F
authors.initials: NF
authors.lastname: Schlecht
authors.forename: Kristin
authors.initials: K
authors.lastname: Elias
authors.forename: Eleanor
authors.initials: E
authors.lastname: Gorbovitsky
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Belbin
authors.forename: Radma
authors.initials: R
authors.lastname: Mahmood
authors.forename: Dwayne
authors.initials: D
authors.lastname: Breining
authors.forename: Hong
authors.initials: H
authors.lastname: Qian
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Childs
authors.forename: Joseph
authors.initials: J
authors.lastname: Locker
authors.forename: Richard
authors.initials: R
authors.lastname: Smith
authors.forename: Missak
authors.initials: M
authors.lastname: Haigentz
authors.forename: Frank
authors.initials: F
authors.lastname: Gunn-Moore
authors.forename: Michael B
authors.initials: MB
authors.lastname: Prystowsky
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007820:Laryngeal Mucosa / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D008565:Membrane Proteins; D008840:Microfilament Proteins / Q000378:metabolism; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D010865:Pilot Projects; D011379:Prognosis; D016019:Survival Analysis; D046888:Tissue Array Analysis; D000099149:Ezrin
chemical_list: D014408:Biomarkers, Tumor; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D000099149:Ezrin; C504793:FRMD6 protein, human; C071058:moesin