Interactions between bovine serum albumin and gemini surfactant alkanediyl-alpha, omega-bis(dimethyldodecyl-ammonium bromide).
Interactions between bovine serum albumin (BSA) and cationic gemini surfactant alkanediyl-alpha,omega-bis(dimethyldodecyl-ammonium bromide) (12-n-12, n=3, 4, 6) in aqueous solution have been investigated by measuring fluorescence, UV-vis transmittance, dynamic lighting scattering, and circular dichroism. Compared to a traditional surfactant dodecyltrimethylammonium bromide (DTAB), 12-n-12 interacts with BSA more strongly. With increasing concentration, 12-n-12 first binds specifically onto BSA leading to the unfolding and aggregation of BSA, and the decrease in alpha-helix content; and then forms micelle-like complexes along the unfolded BSA chains. A gemini surfactant with a longer spacer has a larger effect on BSA unfolding due to a stronger hydrophobic interaction.
Pi
Yingying
Y
Department of Chemistry and Lab for Advanced Materials, East China University of Science and Technology, Shanghai 200237, China.
Shang
Yazhuo
Y
Peng
Changjun
C
Liu
Honglai
H
Hu
Ying
Y
Jiang
Jianwen
J
D000818:Animals; D019281:Dimerization; D057927:Hydrophobic and Hydrophilic Interactions; D008823:Micelles; D011485:Protein Binding; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013057:Spectrum Analysis; D013501:Surface-Active Agents / Q000737:chemistry*
D008823:Micelles; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; C046112:didodecyldimethylammonium; D012710:Serum Albumin, Bovine