title: Gas chromatography-mass spectrometry.
abstract: Gas chromatography-mass spectrometry (GC-MS) has been used in hormone assays particularly for steroids in biological fluids. The combination of GC with MS exploits the high-resolving power of gas chromatography to separate closely related molecules, and the ability of the MS to provide precise data for identification and quantification of the separated substances. GC-MS is a very powerful technique for analysis with specificity of hormones in biological fluids. The general principles of GC-MS are described in this chapter along with some examples that illustrate specific applications of hormone analysis.
authors.affiliation: Department of Clinical Biochemistry, University College London Hospitals, UK.
authors.forename: John W
authors.initials: JW
authors.lastname: Honour
mesh_terms: D000305:Adrenal Cortex Hormones / Q000032:analysis; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D012739:Gonadal Steroid Hormones / Q000032:analysis; D006801:Humans; D013963:Thyroid Hormones / Q000032:analysis
chemical_list: D000305:Adrenal Cortex Hormones; D012739:Gonadal Steroid Hormones; D013963:Thyroid Hormones