title: Effect of ozone produced from antibody-catalyzed water oxidation on pathogenesis of atherosclerosis.
abstract: Recent studies have suggested that antibodies can catalyze the generation of unknown oxidants including hydrogen peroxide (H2O2) and ozone (O3) from singlet oxygen (1O2) and water. This study is aimed to detect the effect of antibody-catalyzed water oxidation on atherosclerosis. Our results showed that both H2O2 and O3 were produced in human leukemia THP-1 monocytes incubated with human immunoglobulin G and phorbol myristate acetate. In the THP-1 monocytes incubated with human immunoglobulin G, phorbol myristate acetate and low density lipoprotein, the intracellular total cholesterol, free cholesterol, cholesteryl ester and lipid peroxides clearly increased, and a larger number of foam cells were observed by oil red O staining. The accumulation of all intracellular lipids was significantly inhibited by vinylbenzoic acid, and only slightly affected by catalase. These findings suggested that the production of O3, rather than H2O2, might be involved in the pathogenesis of atherosclerosis through the antibody-catalyzed water oxidation pathway.
authors.affiliation: Department of Pathophysiology, West China Preclinical Medical and Forensic Medical College, Sichuan University, Chengdu 610041, China.
authors.forename: Ke-Jun
authors.initials: KJ
authors.lastname: Peng
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Huang
authors.forename: Li-Na
authors.initials: LN
authors.lastname: An
authors.forename: Xiao-Qun
authors.initials: XQ
authors.lastname: Han
authors.forename: Jing-Ge
authors.initials: JG
authors.lastname: Zhang
authors.forename: Qiu-Lin
authors.initials: QL
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Shu-Ren
authors.initials: SR
authors.lastname: Wang
mesh_terms: D050197:Atherosclerosis / Q000378:metabolism* / Q000473:pathology*; D001391:Azo Compounds / Q000494:pharmacology; D002384:Catalysis; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D007074:Immunoglobulin G / Q000737:chemistry; D008054:Lipid Peroxides / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D010126:Ozone / Q000494:pharmacology*; D013343:Styrenes / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D001391:Azo Compounds; D007074:Immunoglobulin G; D008054:Lipid Peroxides; D013343:Styrenes; D014867:Water; D010126:Ozone; D002784:Cholesterol; D006861:Hydrogen Peroxide; C011049:oil red O; D010100:Oxygen; C105064:4-vinylbenzoic acid