title: The antioxidative effects of astragalus saponin I protect against development of early diabetic nephropathy.
abstract: It has been known that oxidative stress plays an important role in the development of diabetic nephropathy (DN). The antioxidative effects of Astragalus saponin I (AS I) were studied in vitro and in vivo. In the presence of high glucose and H2O2, the total antioxidative capability, catalase, reduced glutathione, and superoxide dismutase level of rat mesangial cells were significantly decreased, and transforming growth factor beta1 (TGF-beta1) mRNA level, collagen IV, and laminin level were significantly increased. When compared with those in the high glucose group, these 4 indexes of cells incubated in 2.0 and/or 20 micromol/L of AS I were significantly enhanced, and levels of TGF-beta1 mRNA, collagen IV and laminin were statistically decreased. By flowcytomery, percentages of S phase of cells incubated in high glucose and H2O2 were lowered, while those in AS I were increased. Furthermore, the physical behaviors of rats treated with 12 mg/kg of AS I restored with vigor and weight gaining, while the level of HbAlC was significantly reduced. Thus, AS I has antioxidative effects and is a potential compound worth further study because it may prevent the development of DN.
authors.affiliation: Department of Pharmacology, Nanjing Medical University, China.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Yin
authors.forename: Yindi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Junxian
authors.initials: J
authors.lastname: Yu
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
authors.forename: Jianping
authors.initials: J
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Qiu
authors.forename: Haiwei
authors.initials: H
authors.lastname: Wu
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D027883:Astragalus Plant / Q000737:chemistry*; D018929:Cell Culture Techniques; D002460:Cell Line; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D050527:Mesangial Cells / Q000187:drug effects / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000145:classification / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D012503:Saponins