[Effect of cyclophosphamide on spermatogonial stem cells].
OBJECTIVE
To perform a preliminary study on the dysfunction of spermatogonial stem cells (SSCs) induced by cyclophosphamide.


METHODS
According to development stage of spermatogenesis in Wistar rat, 72 rats were divided into three groups, 1, 3 and 9 weeks groups, respectively. Then 24 rats per group were randomly divided into experimental and control groups, and 100 mg/kg cyclophosphamide was injected by intraperitoneal injection in experimental groups, with the same volume of normal saline in the control. Apoptosis of spermatogenic cells were detected after 24 hours by TUNEL. Then 60 one-week rats, whose germ cells were only SSCs, were randomly divided into experimental and control group. We detected c-Kit by immunohistochemistry and cell cycle by flow cytometry at 24 hours, 3 and 9 weeks.


RESULTS
There was no significant difference in apoptosis of germ cells in 1 week between experimental group and control group ( P > 0.05); however, there were significant differences among other groups. The ratio of S stage and c-Kit expression of spermatogonial cells were decreased in one-week rats at 24 hours, 3 and 9 weeks in experimental group compared with the control, respectively (P < 0.01).


CONCLUSIONS
Cyclophosphamide does not significantly induce SSCs apoptosis. It may be more important to interfere the proliferation and differentiation of SSCs.
He
Da-Wei
DW
Department of Pediatric Urology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China. babyuro@163.com
Li
Xu-Liang
XL
Yue
Li-Qin
LQ
Wei
Guang-Hui
GH
Lin
Tao
T
Liu
Jun-Hong
JH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000494:pharmacology*; D008297:Male; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013093:Spermatogonia / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
D003520:Cyclophosphamide; D019009:Proto-Oncogene Proteins c-kit