title: Decreased activity of brush border membrane-bound digestive enzymes in small intestines from pigs experimentally infected with porcine epidemic diarrhea virus.
abstract: Brush border membrane-bound digestive enzymes such as disaccharidases (lactase, sucrase, and maltase), leucine aminopeptidase N, and alkaline phosphatase were measured in jejunum from pigs experimentally infected with porcine epidemic diarrhea virus (PEDV). Three piglets from the infected and control groups were euthanized by electrocution and subjected to necropsy at 24, 36, 48, 60, and 72 hours post-inoculation (hpi). The infection of PEDV to jejunum resulted in significant decreases in brush border membrane-bound digestive enzymes such as disaccharidases (lactase, sucrase, and maltase), leucine aminopeptidase N, and alkaline phosphatase. PEDV replication results in massive destruction of villous enterocytes leading to a marked reduction of intestinal epithelial surface and brush border membrane-bound digestive enzyme activity. Reduced enzymatic activity and villous atrophy in the small intestine is thought to result in a maldigestive and malabsorptive diarrhea.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1, Kwanak-Gu, 151-742 Seoul, Republic of Korea.
authors.forename: K
authors.initials: K
authors.lastname: Jung
authors.forename: K
authors.initials: K
authors.lastname: Ahn
authors.forename: C
authors.initials: C
authors.lastname: Chae
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D003332:Coronaviridae / Q000472:pathogenicity*; D003333:Coronaviridae Infections / Q000378:metabolism / Q000662:veterinary*; D004186:Disaccharidases / Q000378:metabolism; D007583:Jejunum / Q000201:enzymology*; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008871:Microvilli / Q000201:enzymology*; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism*
chemical_list: D000469:Alkaline Phosphatase; D004186:Disaccharidases; D007931:Leucyl Aminopeptidase