title: Rsc4 connects the chromatin remodeler RSC to RNA polymerases.
abstract: RSC is an essential, multisubunit chromatin remodeling complex. We show here that the Rsc4 subunit of RSC interacted via its C terminus with Rpb5, a conserved subunit shared by all three nuclear RNA polymerases (Pol). Furthermore, the RSC complex coimmunoprecipitated with all three RNA polymerases. Mutations in the C terminus of Rsc4 conferred a thermosensitive phenotype and the loss of interaction with Rpb5. Certain thermosensitive rpb5 mutations were lethal in combination with an rsc4 mutation, supporting the physiological significance of the interaction. Pol II transcription of ca. 12% of the yeast genome was increased or decreased twofold or more in a rsc4 C-terminal mutant. The transcription of the Pol III-transcribed genes SNR6 and RPR1 was also reduced, in agreement with the observed localization of RSC near many class III genes. Rsc4 C-terminal mutations did not alter the stability or assembly of the RSC complex, suggesting an impact on Rsc4 function. Strikingly, a C-terminal mutation of Rsc4 did not impair RSC recruitment to the RSC-responsive genes DUT1 and SMX3 but rather changed the chromatin accessibility of DNases to their promoter regions, suggesting that the altered transcription of DUT1 and SMX3 was the consequence of altered chromatin remodeling.
authors.affiliation: Service de Biochimie et Génétique Moléculaire, Bâtiment 144, CEA/Saclay, F-91191 Gif-sur-Yvette Cedex, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Soutourina
authors.forename: Véronique
authors.initials: V
authors.lastname: Bordas-Le Floch
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Gendrel
authors.forename: Amando
authors.initials: A
authors.lastname: Flores
authors.forename: Cécile
authors.initials: C
authors.lastname: Ducrot
authors.forename: Hélène
authors.initials: H
authors.lastname: Dumay-Odelot
authors.forename: Pascal
authors.initials: P
authors.lastname: Soularue
authors.forename: Francisco
authors.initials: F
authors.lastname: Navarro
authors.forename: Bradley R
authors.initials: BR
authors.lastname: Cairns
authors.forename: Olivier
authors.initials: O
authors.lastname: Lefebvre
authors.forename: Michel
authors.initials: M
authors.lastname: Werner
mesh_terms: D002843:Chromatin / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D009154:Mutation; D021122:Protein Subunits / Q000378:metabolism; D014158:Transcription, Genetic; D015003:Yeasts / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; D021122:Protein Subunits; D012321:DNA-Directed RNA Polymerases