Biochemical and biological analyses of Rgr RalGEF oncogene.
The Ras superfamily of GTP-binding proteins is involved in many cellular processes, including cell proliferation, movement, and morphology. One such member, Ral GTPase, activates downstream signaling molecules after a conversion to the active state on GTP binding. The RalGDS-related (Rgr) oncogene belongs to the RalGDS family of guanine nucleotide exchange factors (GEFs). RalGEFs activate Ral by stimulating the dissociation of GDP, allowing the binding of GTP and the initiation of downstream signaling events by Ral effectors. Rgr was first identified as a fusion between the rabbit homolog of the Rad 23 gene and the Rgr gene in a rabbit squamous cell carcinoma. The Rgr portion of the fusion was demonstrated to contain the oncogenic activity. The human form of the Rgr oncogene was identified recently, and expression was detected in human T-cell malignancies. This chapter describes the analysis of rabbit and human Rgr function using various methods. These assays may be used for the study of oncogene function in other systems.
Martello
Laura A
LA
Department of Pathology, New York Cancer Institute, New York University School of Medicine, New York, New York, USA.
Pellicer
Angel
A
D000818:Animals; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D041681:NIH 3T3 Cells; D009857:Oncogenes / Q000502:physiology*; D011817:Rabbits; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism; D020726:ral Guanine Nucleotide Exchange Factor / Q000502:physiology*; D018631:ras Proteins / Q000378:metabolism
D020726:ral Guanine Nucleotide Exchange Factor; D018631:ras Proteins