[Assembly of viral particles in the cells infected with endotheliotropic or fibroblastotropic strains of human cytomegalovirus].
The ultrastructure of endothelial cells and fibroblasts infected with VHL/E or AD169 strains was examined. The cells producing the virus 25 days after infection with VHL/E strains (0.01 PFU/cell) contain numerous cytoplasmic-located filament-associated capsids in addition to standard infectious and noninfectious viral particles. Only single filament-contacting capsids were found in the cytoplasm of the cells producing the virus 20 days after infection with AD169 strain (0.001 PFU/cell). In situ DNA hydridization on the VHL/E-infected cells has indicated that the capsid-contacting filament is human cytomegalovirus DNA. The findings suggest that premature release of viral DNA from the capsids occurs during their maturation in the cytoplasm.
Riaskina
S S
SS
Boiarkin
A V
AV
Bystrevskaia
V B
VB
D002213:Capsid / Q000502:physiology / Q000648:ultrastructure; D002467:Cell Nucleus / Q000821:virology; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000502:physiology* / Q000648:ultrastructure; D003586:Cytomegalovirus Infections / Q000821:virology*; D003593:Cytoplasm / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D004730:Endothelium, Vascular / Q000821:virology*; D005347:Fibroblasts / Q000821:virology*; D006801:Humans; D017403:In Situ Hybridization; D008168:Lung; D008854:Microscopy, Electron; D014471:Umbilical Veins; D019065:Virus Assembly
D004279:DNA, Viral