title: [PPARgamma1 overexpression on caveolin-1 expression of Raw264.7 cells].
abstract: OBJECTIVE
  To investigate the effect of PPARgamma1 gene overexpression on caveolin-1 mRNA and protein expressions in a murine macrophage cell line Raw264.7.
  
  
  METHODS
  Replication-deficient recombinant adenovirus expression vector of PPARgamma1 was constructed using the AdEasy system. Raw264.7 cells were randomly treated as follows: P group (PPARgamma1 gene overexpression), T group (Troglitazone 40 micromol/L in DMSO), PT group (PPARgamma1 gene overexpression and Troglitazone) and control group. Changes of PPARgamma1 and caveolin-1 at mRNA and protein levels were investigated.
  
  
  RESULTS
  Caveolin-1 expression can be detected by RT-PCR in Raw264.7, by immunocytochemistry method in cell and nuclear membrane but not by immunoblotting at protein level. Caveolin-1 expression at mRNA and protein levels in Raw264.7 were significantly higher in P, T and PT groups compared to control group and the expression was also significantly higher in PT group than that in P group and T group (P < 0.05). PPARgamma expression was significantly increased in PT group and P group where remained unchanged in T group compared to control group.
  
  
  CONCLUSIONS
  PPARgamma1 overexpression can upregulate caveolin-1 expression in macrophages. Troglitazone upregulated caveolin-1 expression in the absence of increased PPARgamma1 expressions at mRNA and protein levels.
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Hu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chun-xi
authors.initials: CX
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Ma
authors.initials: M
authors.lastname: Jing
authors.forename: Hong
authors.initials: H
authors.lastname: He
authors.forename: Jin-bo
authors.initials: JB
authors.lastname: Feng
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Gui-hua
authors.initials: GH
authors.lastname: Jiang
authors.forename: Xian-jun
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D051242:Caveolin 1 / Q000378:metabolism*; D002460:Cell Line; D002839:Chromans / Q000494:pharmacology; D015870:Gene Expression; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D047495:PPAR gamma / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D045162:Thiazolidinediones / Q000494:pharmacology; D000077288:Troglitazone
chemical_list: D051242:Caveolin 1; D002839:Chromans; D047495:PPAR gamma; D012333:RNA, Messenger; D045162:Thiazolidinediones; D000077288:Troglitazone