title: Conjunctival papilloma and human papillomavirus: identification of HPV types by PCR.
abstract: OBJECTIVE
  To report the identification of human papillomavirus types in four cases of conjunctival papillomas and to review the literature regarding human conjunctival papillomavirus (HPV).
  
  
  METHODS
  Specimens from conjunctival papillomas of four patients were analyzed for the presence of HPV by polymerase chain reaction and subsequent filter hybridization. HPV types 6, 11, 16, 18, 31, and 33 were investigated. Histologic sections were analyzed for the presence of koilocytosis.
  
  
  RESULTS
  Histologic examination confirmed HPV infection in all cases. HPV type 11 was detected in all specimens.
  
  
  CONCLUSIONS
  HPV is frequently implicated in the pathogenesis of proliferative squamous lesions. HPV type 11 was the most frequently found in benign conjunctival lesion in this study.
authors.affiliation: Department of Ophthalmology, University of Roma 'Campus Bio-Medico', Roma, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Minchiotti
authors.forename: L
authors.initials: L
authors.lastname: Masucci
authors.forename: M
authors.initials: M
authors.lastname: Serapiao Dos Santos
authors.forename: E
authors.initials: E
authors.lastname: Perrella
authors.forename: R
authors.initials: R
authors.lastname: Graffeo
authors.forename: A
authors.initials: A
authors.lastname: Lambiase
authors.forename: S
authors.initials: S
authors.lastname: Bonini
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D003230:Conjunctival Neoplasms / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D052140:Human papillomavirus 11 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D010212:Papilloma / Q000473:pathology / Q000821:virology*; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004279:DNA, Viral