[Comparison of three staining methods for hepatitis B virus].
OBJECTIVE
To compare the advantages and disadvantages of three staining methods of HBV.


METHODS
Normal Liver tissue and HBV-infected, HCV-infected, or dually infected (HBV and HCV) liver tissues were selected for this study. Formalin-fixed, paraffin-embedded sections (4 microm) were prepared. Each of the liver tissue specimens was detected by three staining methods, including immunohistochemical methods, Shikata's orcein stain and Victoria blue stain,respectively.


RESULTS
In the three methods, all of six HBV-infected cases showed intense staining, and three cases with dual infection (HBV and HCV) were weakly positive. However, both normal and HCV-infected liver tissues showed no staining. HBsAg stained dark brown with Immunohistochemical stain; HBsAg containing ground-glass hepatocytes stained magenta with Shikata's orcein stain; HBsAg stained blue with Victoria blue.


CONCLUSIONS
Each of three methods has its own advantages and disadvantages: high specificity and sensitivity, but high cost for immunohistochemical methods; complicated and overelaborate procedure for preparation of solutions, lower specificity and sensitivity, but low cost, for special staining methods.
Du
Juan
J
Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100083, China. Dujuan.122@eyou.com
Han
Zhi-hui
ZH
Pei
Fei
F
Zheng
Jie
J
D006509:Hepatitis B / Q000175:diagnosis / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006515:Hepatitis B virus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D009930:Organic Chemicals / Q000737:chemistry; D010078:Oxazines / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*
D006514:Hepatitis B Surface Antigens; D009930:Organic Chemicals; D010078:Oxazines; C041370:victoria blue; C002313:PAcein