title: [Real-time fluorescence quantitative PCR in detecting ribosome protein S13 (RPS13) gene expression in NK/T cell lymphoma].
abstract: OBJECTIVE
  To quantitatively detect the expression level of ribosome protein S13 (RPS13) in NK/T cell lymphoma.
  
  
  METHODS
  The total RNA isolated from the parafin-embedded tissue of NK/T cell lymphoma was reversely transcribed into cDNA. The real-time fluorescence quantitative PCR technology method was used to analyze the expression level of RPS13 gene.
  
  
  RESULTS
  The real-time fluorescence quantitative PCR technology was successfully performed to precisely detect the mRNA level. The expression level of RPS13 gene in tumor tissue of 20 cases of NK/T cell lymphoma was dramatically lower than that in normal peripheral blood lymphocyte of healthy controls.
  
  
  CONCLUSIONS
  The RPS13 gene has lower expression level in tumor tissue cells of NK/T cell lymphoma than in normal lymphocyte, indicating that it plays an role in the development of the NK/T cell lymphoma.
authors.affiliation: Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Liu
authors.forename: Miao-xia
authors.initials: MX
authors.lastname: He
authors.forename: Qiong-lan
authors.initials: QL
authors.lastname: Tang
authors.forename: Sha
authors.initials: S
authors.lastname: Zhao
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Zhang
authors.forename: Qing-jie
authors.initials: QJ
authors.lastname: Xia
authors.forename: Gan-di
authors.initials: GD
authors.lastname: Li
mesh_terms: D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000378:metabolism*; D009669:Nose Neoplasms / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012269:Ribosomal Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics
chemical_list: C047013:RPS13 protein, human; D012269:Ribosomal Proteins