[Expression of fragile histidine triad (FHIT) protein and its significance in diagnosing classical Hodgkin lymphoma].
OBJECTIVE
To study the expression of FHIT protein and its potential application in diagnosing classic Hodgkin lymphoma.


METHODS
Immunohistochemical study using EnVision method for FHIT tumor suppressor protein, hematopoietic stem cell markers CD133/AC133 and CD34, B-cell marker CD20, T-cell marker CD3 and oncoprotein c-erbB2 was performed on 33 cases of classic Hodgkin lymphoma.


RESULTS
Thirty-three of the Hodgkin lymphoma cases (90.9%) expressed FHIT protein. The antigen was mainly located in the cytoplasm, nucleus and membrane of classic Reed-Sternberg and Reed-Sternberg-like cells. Normal B and T lymphocytes, as well as their malignant counterparts, were negative for FHIT protein; whereas monocytes, histiocytes and dendritic cells were positive. All the cases studied were negative for CD133/AC133, CD34, CD3 and c-erbB-2. Two of the 33 cases showed positive staining for CD20 in some of the Reed-Sternberg cells.


CONCLUSIONS
The expression of FHIT protein can be used as a useful adjunct in diagnosing classic Hodgkin lymphoma.
Zhao
Po
P
Department of Pathology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China. zhaopo@301hospital.com.cn
Lü
Ya-li
YL
Zhong
Mei
M
Chen
Ling-hong
LH
Pu
Xiao-lu
XL
D000071916:AC133 Antigen; D017766:Acid Anhydride Hydrolases / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism; D018951:Antigens, CD20 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006689:Hodgkin Disease / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D016539:Reed-Sternberg Cells / Q000378:metabolism; D012680:Sensitivity and Specificity; D000099170:Bis(5'-adenosyl)triphosphatase
D000071916:AC133 Antigen; D017766:Acid Anhydride Hydrolases; D015703:Antigens, CD; D018951:Antigens, CD20; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D009363:Neoplasm Proteins; D010455:Peptides; D000099170:Bis(5'-adenosyl)triphosphatase; C000606023:PROM1 protein, human