Isolation and identification of 2alpha,25-dihydroxyvitamin D3, a new metabolite from Pseudonocardia autotrophica 100U-19 cells incubated with Vitamin D3.
Pseudonocardia autotrophica converted Vitamin D(3) to 25-hydroxyvitamin D(3) and 1alpha,25-dihydroxyvitamin D(3). The hydroxylation of Vitamin D(3) with P. autotrophica was enhanced by the addition of cyclodextrin. In this microbial hydroxylation, a new Vitamin D(3) metabolite was observed in the reaction mixture of P. autotrophica and Vitamin D(3), and was isolated in a pure form by several steps of chromatography. The structure of the new metabolite was determined to be 2alpha,25-dihydroxyvitamin D(3) by UV, NMR and mass spectroscopic analyses. Biological evaluation of the new metabolite was conducted by means of several experiments.
Takeda
Koji
K
Bioresource Laboratories, Mercian Corporation, Iwata, Shizuoka, Japan. takeda-k@mercian.co.jp
Kominato
Kaichiro
K
Sugita
Atsuko
A
Iwasaki
Yukiko
Y
Shimazaki
Mika
M
Shimizu
Masato
M
D000818:Animals; D019556:COS Cells / Q000187:drug effects; D002112:Calcifediol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D002352:Carrier Proteins; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D002762:Cholecalciferol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D005786:Gene Expression Regulation; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D006900:Hydroxylation; D015603:Keratinocytes / Q000187:drug effects; D008954:Models, Biological; D008958:Models, Molecular; D008968:Molecular Conformation; D009615:Nocardia / Q000737:chemistry* / Q000187:drug effects*; D020033:Protein Isoforms / Q000302:isolation & purification* / Q000494:pharmacology; D018167:Receptors, Calcitriol / Q000378:metabolism; D013329:Structure-Activity Relationship
D002352:Carrier Proteins; D020033:Protein Isoforms; D018167:Receptors, Calcitriol; D002762:Cholecalciferol; D002112:Calcifediol