title: An 8bp indel in exon 1 of Ghrelin gene associated with chicken growth.
abstract: Ghrelin, acts as the endogenous ligand for growth hormone secretagogues receptor (GHS-R), is a novel growth hormone (GH) releasing peptide with reported effects on food intake in chickens. In this study, an 8 bp indel polymorphism in exon 1 of the chicken Ghrelin (cGHRL) gene was genotyped in a F(2) designed full-sib population to analyze its associations with chicken growth and carcass traits. Later, mRNA level in the proventriculus was determined by real-time PCR to reveal the expression feature of cGHRL gene. Result showed that this 8 bp indel was significantly associated with body weight at the age of 28 days (BW28) and 56 days (BW56), eviscerated weight (EW) and leg muscle weight (LMW) (P<0.05), highly significantly associated with hatch weight (HW), BW14, 21, 35, 42, 49, 90 and body length (BL), dressed weight (DW), eviscerated weight with giblet (EWG), wing weight (WW), breast muscle weight (BMW) and head and neck weight (HNW) (P<0.01). Meanwhile, A allele (with 'CTAACCTG') was positive for chicken growth as individuals with AA genotype had the highest value of all traits. Analysis on cGhrelin mRNA level revealed that it differed significantly among individuals with three genotypes (P<0.05). Individuals with AB genotype had the highest mRNA level, whereas that of AA had the lowest one. It was concluded that this 8 bp indel of cGHRL gene was significantly associated with most body weight and body composition traits, and negative effect of endogenous Ghrelin on chicken growth were indicated by this study.
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China.
authors.forename: Meixia
authors.initials: M
authors.lastname: Fang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Nie
authors.forename: Chenglong
authors.initials: C
authors.lastname: Luo
authors.forename: Dexiang
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiquan
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001665:Binding Sites; D001823:Body Composition / Q000235:genetics; D001835:Body Weight / Q000235:genetics; D002645:Chickens / Q000235:genetics / Q000254:growth & development*; D005091:Exons; D017353:Gene Deletion; D005838:Genotype; D054439:Ghrelin; D016254:Mutagenesis, Insertional; D036361:Peptide Hormones / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011531:Proventriculus / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D054439:Ghrelin; D036361:Peptide Hormones