title: Molecular epidemiology of dengue in the Pacific: introduction of two distinct strains of dengue virus type-1 [corrected] into Hawaii.
abstract: In 2000, a major dengue epidemic, caused by the type-1 virus (DENV-1), began in the Pacific and Asia, with cases still being reported in 2006. The phylogenetic analysis of full-length sequences of the envelope-protein gene of DENV-1 isolates recovered during outbreaks in Hawaii and Tahiti in 2001-2002 indicated that most Hawaiian isolates were Tahitian in origin. All the Hawaiian and Tahitian isolates were identified as the Pacific subtype (i.e. subtype IV) of DENV-1. A Hawaiian isolate, collected from a resident who had travelled to Samoa, differed significantly at the nucleotide level, however, from all the other Hawaiian strains, clustering, in the phylogenetic analysis, with a virus previously isolated from another visitor to Samoa. These results not only indicate that two distinct strains of DENV-1 were introduced into Hawaii in 2001 but also illustrate the ease with which dengue can be carried across distances of many thousands of miles.
authors.affiliation: University of Hawaii at Manoa, John A. Burns School of Medicine, Biomedical Science Building Room D-204, 1960 East West Road, Honolulu, HI 96822, USA. imrie@pbrc.hawaii.edu
authors.forename: A
authors.initials: A
authors.lastname: Imrie
authors.forename: Z
authors.initials: Z
authors.lastname: Zhao
authors.forename: S N
authors.initials: SN
authors.lastname: Bennett
authors.forename: P
authors.initials: P
authors.lastname: Kitsutani
authors.forename: M
authors.initials: M
authors.lastname: Laille
authors.forename: P
authors.initials: P
authors.lastname: Effler
mesh_terms: D003715:Dengue / Q000453:epidemiology / Q000235:genetics*; D003716:Dengue Virus / Q000235:genetics*; D004196:Disease Outbreaks; D005814:Genes, Viral / Q000235:genetics; D006254:Hawaii; D006801:Humans; D009154:Mutation; D010802:Phylogeny; D011114:Polynesia / Q000453:epidemiology; D017422:Sequence Analysis, DNA / Q000379:methods; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins