title: Increased expression of interleukin-10 in unstable plaque obtained by directional coronary atherectomy.
abstract: OBJECTIVE
  The present study investigates the expression and localization of interleukin (IL)-10, an important anti-inflammatory cytokine, in atherectomy specimens from patients with stable and unstable angina.
  
  
  RESULTS
  Twenty-two patients with stable angina and 21 with unstable angina who underwent directional coronary atherectomy for de novo lesions were studied. The atherectomy specimens were morphologically assessed and immunohistochemically stained with antibodies for IL-10, macrophages, smooth muscle cells, and endothelial cells. The localization and immunopositive areas were evaluated using an image analysing system. Immunoreactivity for IL-10 was detected in coronary plaques, especially in macrophages. Immunopositive areas of macrophages and IL-10, as well as the incidence of thrombus formation, were significantly greater in specimens from patients with unstable angina than in those from patients with stable angina (macrophages, P<0.001; IL-10, P<0.05; thrombus formation, P<0.05; respectively). Even after adjustment, IL-10 expression and the incidence of thrombus formation were significantly greater in the unstable angina group (P<0.05, each). The immunoreactivities for smooth muscle cells and endothelial cells did not differ between the two groups.
  
  
  CONCLUSIONS
  IL-10 was more frequently expressed in specimens from patients with unstable angina. This finding might contribute to a better understanding of plaque instability.
authors.affiliation: First Department of Internal Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan.
authors.forename: Kensaku
authors.initials: K
authors.lastname: Nishihira
authors.forename: Takuroh
authors.initials: T
authors.lastname: Imamura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamashita
authors.forename: Kinta
authors.initials: K
authors.lastname: Hatakeyama
authors.forename: Yoshisato
authors.initials: Y
authors.lastname: Shibata
authors.forename: Yoshitatsu
authors.initials: Y
authors.lastname: Nagatomo
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Date
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kita
authors.forename: Tanenao
authors.initials: T
authors.lastname: Eto
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Asada
mesh_terms: D000368:Aged; D000787:Angina Pectoris / Q000378:metabolism*; D000789:Angina, Unstable / Q000378:metabolism; D017225:Atherectomy, Coronary; D003324:Coronary Artery Disease / Q000378:metabolism*; D003328:Coronary Thrombosis / Q000209:etiology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D016753:Interleukin-10