title: Genetic characterization of novel reassortant H1N2 influenza A viruses isolated from pigs in southeastern China.
abstract: In December 2004, three influenza H1N2 viruses were isolated from lung samples of pigs that had died from respiratory disease on a farm in southeastern China. To determine the genetic characterization and probable origin, one of the three isolates, A/Swine/Zhejiang/1/2004 (Sw/ZJ/1/2004), was genetically analyzed. Sw/ZJ/1/2004 was a reassortant with an NA gene most closely related to the corresponding gene from a human-like H3N2 virus circulating in 1995. The remaining seven genes were most closely related to those from the classical swine H1N1 virus. Sw/ZJ/1/2004 appeared to be a novel reassortant H1N2 virus that was genetically distinguishable from other H1N2 viruses found in pigs worldwide. The isolation of Sw/ZJ/1/2004 provided further evidence for pigs serving as a "mixing vessel" for the generation of new reassortant genotypes of influenza viruses and emphasizes the importance of reinforcing influenza virus surveillance in pigs in China.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: X
authors.initials: X
authors.lastname: Qi
authors.forename: C P
authors.initials: CP
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics*; D009980:Influenza A virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008168:Lung / Q000821:virology; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics*; D012141:Respiratory Tract Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000821:virology*