Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two- dimensional scanning mirror.
Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm x 0.75 mm in size and driven in two dimensions by microfabricated vertical comb electrostatic actuators can provide optical deflection angles through a range of approximately16 degrees . Using such scanners we demonstrated two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.52 kHz.
Piyawattanametha
Wibool
W
James H. Clark Center for Biomedical Engineering & Sciences, Stanford University, Stanford, California 94305, USA.
Barretto
Robert P J
RP
Ko
Tony H
TH
Flusberg
Benjamin A
BA
Cocker
Eric D
ED
Ra
Hyejun
H
Lee
Daesung
D
Solgaard
Olav
O
Schnitzer
Mark J
MJ
D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007909:Lenses; D019563:Mechanics; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity