Antiserum against perimicrovillar membranes and midgut tissue reduces the development of Trypanosoma cruzi in the insect vector, Rhodnius prolixus.
Antiserum raised against Rhodnius prolixus perimicrovillar membranes (PMM) and midgut tissue interfered with the midgut structural organization and reduced the development of Trypanosoma cruzi in the R. prolixus insect vector. SDS-PAGE and Western blot analyses confirmed the specific recognition of midgut proteins by the antibody. Feeding, mortality, molt, and oviposition of the insects were unaffected by feeding with the antiserum. However, the eclosion of the eggs were reduced from R. prolixus females treated with antiserum. Additionally, in vivo evaluation showed that after oral treatment with the antiserum, the intensity of infection with the Dm-28c clone of T. cruzi decreased in the digestive tract of fifth-instar nymphs and in the excretions of R. prolixus adults. These results suggest that the changes observed in the PMM organization in the posterior midgut of R. prolixus may not be important for triatomine survival but the antiserum acts as a transmission-reduction vaccine able to induce significant decreases in T. cruzi infection in the vector.
Gonzalez
M S
MS
Departamento de Biologia Geral, Universidade Federal Fluminense, Morro do Valonguinho s/n, Niterói, Cx Postal 100436, CEP 24001-970, Rio de Janeiro, Brazil.
Hamedi
A
A
Albuquerque-Cunha
J M
JM
Nogueira
N F S
NF
De Souza
W
W
Ratcliffe
N A
NA
Azambuja
P
P
Garcia
E S
ES
Mello
C B
CB
D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006790:Host-Parasite Interactions / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007303:Insect Vectors / Q000276:immunology* / Q000469:parasitology* / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D008871:Microvilli / Q000276:immunology / Q000648:ultrastructure; D011817:Rabbits; D012239:Rhodnius / Q000276:immunology* / Q000469:parasitology* / Q000648:ultrastructure; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000276:immunology*
D007106:Immune Sera