Crystallization and preliminary X-ray diffraction analysis of a tRNASer acceptor-stem microhelix.
In order to understand elongator tRNA(Ser) and suppressor tRNA(Sec) identity elements, the respective acceptor-stem helices have been synthesized and crystallized in order to analyse and compare their structures in detail at high resolution. The synthesis, crystallization and preliminary X-ray diffraction results for a seven-base-pair tRNA(Ser) acceptor-stem helix are presented here. Diffraction data were collected to 1.8 A, applying synchrotron radiation and cryogenic cooling. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 36.14, b = 38.96, c = 30.81 A, beta = 110.69 degrees .
Förster
Charlotte
C
Institute of Chemistry and Biochemistry, Free University Berlin, Thielallee 63, 14195 Berlin, Germany.
Krauss
Norbert
N
Brauer
Arnd B E
AB
Szkaradkiewicz
Karol
K
Brode
Svenja
S
Hennig
Klaus
K
Fürste
Jens P
JP
Perbandt
Markus
M
Betzel
Christian
C
Erdmann
Volker A
VA
D001483:Base Sequence; D003460:Crystallization; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D012362:RNA, Transfer, Ser / Q000737:chemistry*; D014961:X-Ray Diffraction
D009841:Oligonucleotides; D012362:RNA, Transfer, Ser