title: Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein.
abstract: Two recombinant antigens which individually protect mice from lethal intranasal infection were studied in combination, either as a mixture of two separately expressed proteins or as a single chimeric expression product. Mice vaccinated with either combination survived longer than mice given single antigens. Immunized mice also exhibited specific IgG immunoglobulins and yielded splenocytes which produced interferon-gamma in response to either antigen. The chimeric antigen has the practical advantage of offering enhanced protection from multiple components without increasing production costs.
authors.affiliation: The Valley Fever Center for Excellence, University of Arizona, Tucson, AZ 85721, USA. lfshubit@u.arizona.edu
authors.forename: Lisa F
authors.initials: LF
authors.lastname: Shubitz
authors.forename: Jieh-Juen
authors.initials: JJ
authors.lastname: Yu
authors.forename: Chiung-Yu
authors.initials: CY
authors.lastname: Hung
authors.forename: Theo N
authors.initials: TN
authors.lastname: Kirkland
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Robert
authors.initials: R
authors.lastname: Perrill
authors.forename: Julie
authors.initials: J
authors.lastname: Simons
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xue
authors.forename: Roger A
authors.initials: RA
authors.lastname: Herr
authors.forename: Garry T
authors.initials: GT
authors.lastname: Cole
authors.forename: John N
authors.initials: JN
authors.lastname: Galgiani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003045:Coccidioides; D003047:Coccidioidomycosis / Q000382:microbiology / Q000517:prevention & control*; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000627:therapeutic use*; D005657:Fungal Vaccines / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000517:prevention & control*; D022223:Vaccines, Subunit / Q000627:therapeutic use*; D014614:Vaccines, Synthetic / Q000627:therapeutic use
chemical_list: D005656:Fungal Proteins; D005657:Fungal Vaccines; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic