Nongastric Helicobacter species detected in the intestinal tract of children.
The presence of members of the family Helicobacteraceae in the colon of children was investigated using PCR, denaturing gradient gel electrophoresis, and fluorescent in situ hybridization. The rate of detection of species belonging to the Helicobacteraceae family in children with inflammatory bowel disease (92%) and irritable bowel syndrome (100%) was found to be significantly higher than that in healthy children (25%) (P < 0.05).
Zhang
Li
L
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Day
Andrew
A
McKenzie
Gavin
G
Mitchell
Hazel
H
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003106:Colon / Q000382:microbiology*; D004586:Electrophoresis / Q000379:methods; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000382:microbiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015212:Inflammatory Bowel Diseases / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction; D016968:Wolinella / Q000235:genetics / Q000302:isolation & purification*