title: Dissemination of Salmonella enterica serotype agona and multidrug-resistant Salmonella enterica serotype typhimurium in Cuba.
abstract: The molecular epidemiology, antimicrobial susceptibility, and mechanisms of resistance of 34 Salmonella spp. strains causing acute gastroenteritis, isolated from different provinces in Cuba, were determined. Sixty-four percent of the strains showed multiresistance. Salmonella typhimurium was the most frequent with 15 strains (44%), 13 of which belonged to phagotype 104 and presented similar genetic profiles of pulsed field gel electrophoresis. High levels of resistance to tetracycline (53%), spectinomycin (50%), ampicillin (44%), and chloramphenicol (41%) were found. Resistance to tetracycline was associated with the tet G and tet A genes. Resistance to ampicillin was caused by the presence of beta-lactamases, mainly the CARB type. The floR gene was the main mechanism of resistance to chloramphenicol. Our results showed an antimicrobial susceptible clone of Salmonella enterica serotype Agona in two separate regions. This is the first report of the widespread dissemination of a multiresistant clone of S. enterica serotype Typhimurium definitive phage type 104 in Cuba.
authors.affiliation: Servicio de Microbiología, IDIBAPS, Hospital Clínic, Barcelona, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Cabrera
authors.forename: Joaquim
authors.initials: J
authors.lastname: Ruiz
authors.forename: Margarita
authors.initials: M
authors.lastname: Ramírez
authors.forename: Laura
authors.initials: L
authors.lastname: Bravo
authors.forename: Anabel
authors.initials: A
authors.lastname: Fernández
authors.forename: Ana
authors.initials: A
authors.lastname: Aladueña
authors.forename: Aurora
authors.initials: A
authors.lastname: Echeíta
authors.forename: Joaquim
authors.initials: J
authors.lastname: Gascón
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Alonso
authors.forename: Jordi
authors.initials: J
authors.lastname: Vila
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017920:Antiporters / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001434:Bacteriophage Typing; D001435:Bacteriophages / Q000145:classification; D003462:Cuba / Q000453:epidemiology; D018432:Drug Resistance, Multiple / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D029968:Escherichia coli Proteins / Q000235:genetics; D005843:Geography; D006801:Humans; D032023:Integrons / Q000235:genetics; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D012480:Salmonella Infections / Q000453:epidemiology* / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D012703:Serotyping; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D017920:Antiporters; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C501927:FloR protein, E coli; C079051:tetA protein, Bacteria; D001618:beta-Lactamases