title: The effects of fumonisin B1 on viability and mitogenic response of avian immune cells.
abstract: Fumonisin B1 (FB1) is a mycotoxin produced by the fungus Fusarium verticillioides (formerly Fusarium moniliforme) and is found in diverse crops such as corn, wheat, and barley. Many diseases linked to FB1, such as porcine pulmonary edema, rat hepatic cancer, and equine leukoencephalomalacia, indicate a compromised immune system. The purpose of this study was to determine whether FB1 altered immunological responses in various cell populations of Single Comb White Leghorn chicks. Cells collected for this study were obtained from those immunological organs with well-defined responses (i.e., spleen, thymus, and blood). Cell populations were exposed to 5 to 50 microg/mL FB1 in vitro for 24 to 72 h, and viability and mitogenic response were evaluated. The effects of FB1 on the mitogenic response were evaluated in cell populations from the spleen and blood stimulated with the mitogens, lipopolysaccharide, concanavalin A, and pokeweed mitogen and in thymocytes stimulated with concanavalin A. The 3-(4,5-dimethylthazol-2-yl)-diphenyl-2H-tetrazolium bromide (MTT) reduction assay was used to assess viability and mitogenic response. Fumonisin B1 decreased spleen cell viability and mitogenic response, albeit the degree of decrease varied with mitogen and time of exposure. Fumonisin B1 increased number of viable thymic cells at 50 microg/mL but had no effect on the mitogenic response of thymocytes. Fumonisin B1 had no effect on blood lymphocyte viability or mitogenic response.
authors.affiliation: Department of Animal and Veterinary Sciences, Clemson University, SC 29634, USA. becky.keck@hgtc.edu
authors.forename: B B
authors.initials: BB
authors.lastname: Keck
authors.forename: A B
authors.initials: AB
authors.lastname: Bodine
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D037341:Fumonisins / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D008934:Mitogens / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
chemical_list: D037341:Fumonisins; D008070:Lipopolysaccharides; D008934:Mitogens; D011043:Pokeweed Mitogens; D003208:Concanavalin A; C056933:fumonisin B1