Urea hydrogen peroxide determination in whole blood using europium tetracycline probe.
We introduce the use of a lanthanide complex, tetracycline-europium, for the clinical diagnosis of urea hydrogen peroxide in human whole blood. The values obtained agree with the urea concentration variation verified in 49 patients, including 12 predialysis, 12 peritoneal, and 15 dialysis subjects, and 10 controls. This method is noninvasive and can help in the identification of renal and cardiac diseases.
Courrol
Lilia C
LC
FATEC-SP/CEETEPS, São Paulo, SP, Brazil. lcourrol@fatecsp.br
Bellini
Maria H
MH
Tarelho
Luiz V G
LV
Silva
Flávia R O
FR
Mansano
Ronaldo D
RD
Gomes
Laércio
L
Vieira
Nilson D
ND
Shor
Nestor
N
D015374:Biosensing Techniques / Q000379:methods; D000077463:Carbamide Peroxide; D004338:Drug Combinations; D005453:Fluorescence; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000175:diagnosis / Q000628:therapy; D015335:Molecular Probes / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010545:Peroxides / Q000097:blood*; D006435:Renal Dialysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013754:Tetracyclines / Q000737:chemistry*; D014508:Urea / Q000031:analogs & derivatives* / Q000097:blood
D004338:Drug Combinations; D015335:Molecular Probes; D009942:Organometallic Compounds; D010545:Peroxides; D013754:Tetracyclines; C488317:europium(III) tetracycline; D000077463:Carbamide Peroxide; D014508:Urea