Delivery of condensed DNA by liposomal non-viral gene delivery system into nucleus of dendritic cells.
In this study, we developed novel double-membranous non-viral gene delivery system modified with SV-40 T antigen-derived nuclear localization signal (NLS-DMEND) for delivery of luciferase plasmid DNA to nucleus of non-dividing mouse bone marrow-derived dendritic cells (BMDC). Intracellular trafficking and gene expression of NLS-DMEND in the BMDC were evaluated. Condensed DNA was observed in the nucleus by confocal laser scanning microscopy, and the NLS-DMEND induced significant luciferase activity in the BMDC. It was suggested that the condensed DNA particle transferred into nucleus via energy dependent manner, since the nuclear transfer was inhibited by metabolic inhibitors. In conclusion, condensed plasmid DNA was delivered into the nucleus of non-dividing BMDC by NLS-DMEND.
Nakamura
Takashi
T
Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Moriguchi
Rumiko
R
Kogure
Kentaro
K
Minoura
Arisa
A
Masuda
Tomoya
T
Akita
Hidetaka
H
Kato
Kazunori
K
Hamada
Hirofumi
H
Ueno
Masaharu
M
Futaki
Shiroh
S
Harashima
Hideyoshi
H
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D003713:Dendritic Cells / Q000378:metabolism*; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes; D008156:Luciferases / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D019913:Nuclear Localization Signals; D010957:Plasmids
D008081:Liposomes; D019913:Nuclear Localization Signals; D004247:DNA; D008156:Luciferases