The expression of the serine proteinase gene of Bacillus intermedius in Bacillus subtilis.
The gene encoding for Bacillus intermedius serine proteinase was cloned and the complete nucleotide sequence was determined. Gene expression was explored in the protease-deficient strain Bacillus subtilis AJ73 during different stages of growth. Catabolite repression involved in control of proteinase expression during transition state and onset of sporulation was not efficient at the late stationary phase. Salt stress leads to induction of serine proteinase production during B. subtilis AJ73(pCS9) post-exponential growth. Expression of proteinase in B. subtilis deg-mutants may be controlled by DegU regulator. B. subtilis spo0-mutants failed to accomplish B. intermedius proteinase production. These data suggest complex network regulation of B. intermedius serine proteinase expression, including the action of spo0, degU, catabolite repression and demonstrate changes in control of enzyme biosynthesis at different stages of growth.
Sharipova
Margarita
M
Department of Microbiology, Kazan State University, Kazan, Russia. Margarita.Sharipova@ksu.ru
Balaban
Nelly
N
Kayumov
Airat
A
Kirillova
Yuliya
Y
Mardanova
Ayslu
A
Gabdrakhmanova
Leila
L
Leshchinskaya
Inna
I
Rudenskaya
Galina
G
Akimkina
Tatiana
T
Safina
Dina
D
Demidyuk
Ilya
I
Kostrov
Sergey
S
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D003470:Culture Media; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics*; D020860:Subtilisin
D003470:Culture Media; D012697:Serine Endopeptidases; D020860:Subtilisin