title: [Cloning and sequencing of the GTP cyclohydrolase I function region of azole target enzyme gene in artificially induced resistant Candida albicans].
abstract: OBJECTIVE
  To study the relationship between gene mutation of GTP cyclohydrolase I function region in lanosterine 14 alpha-demethylase (14-DM) DNA sequence and drug resistance of Candida albicans.
  
  
  METHODS
  One standard strain and 2 isolate strains of Candida albicans were induced artificially by fluconazole plus albendazole. The gene fragments of the 3 strains and another 2 clinical isolates which were resistant to fluconazol were detected by PCR, and then cloned onto pMD-18T vectors to sequence and analyze the change of gene sequence after the induction.
  
  
  RESULTS
  The sequences underwent substantial gene mutations after induction. Some of the mutations resulted in alteration of amino acids. The sequence change and subsequent alteration of amino acids in the tested strains coincided with those in the clinical isolates.
  
  
  CONCLUSIONS
  Gene mutation and alteration of amino acid of 14-DM GTP domain are related to azole-resistance in Candida albicans.
authors.affiliation: Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Xu-liang
authors.initials: XL
authors.lastname: Deng
authors.forename: Xin-cheng
authors.initials: XC
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Ruo-yu
authors.initials: RY
authors.lastname: Li
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Lu
authors.forename: Wen-qi
authors.initials: WQ
authors.lastname: He
authors.forename: Li-guo
authors.initials: LG
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Fan
authors.forename: Xiao-yang
authors.initials: XY
authors.lastname: Hu
mesh_terms: D015766:Albendazole / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology; D006136:GTP Cyclohydrolase / Q000235:genetics*; D009154:Mutation / Q000187:drug effects; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D015725:Fluconazole; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases; D006136:GTP Cyclohydrolase; D015766:Albendazole