title: Narcissus symptomless virus: a new carlavirus of daffodils.
abstract: A filamentous virus, with particles 600-650 nm long, was purified from Narcissus pseudonarcissus (daffodil) in Hangzhou and an antiserum prepared. After mechanical inoculation, the virus could be detected serologically in Narcissus species but not in some commonly used virus indicators. Infection was symptomless. The complete sequence of the genomic RNA (8281 nt) showed six predicted ORFs typical of carlaviruses. Pairwise comparisons of gene sequences and phylogenetic analysis demonstrated that the new virus should be classified as a carlavirus but that it was not closely related to members of any current species. We propose the name Narcissus symptomless virus (NSV).
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, PR China. jchen1975@zjip.com
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: Y-H
authors.initials: YH
authors.lastname: Shi
authors.forename: Y-W
authors.initials: YW
authors.lastname: Lu
authors.forename: M J
authors.initials: MJ
authors.lastname: Adams
authors.forename: J-P
authors.initials: JP
authors.lastname: Chen
mesh_terms: D017788:Carlavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D031427:Narcissus / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins