[Induction of apoptosis of tumor multidrug resistance cell by uvarigrin and its mechanism].
OBJECTIVE
To study the effect of uvarigrin on mitochondrial dependent pathway during the apoptosis induced by it in MDR KBv200 cells and their parental sensitive KB cells.


METHODS
MTT assay was used to detect the cytotoxic effect of uvarigrin on KBv200 and KB cells. Annexin V FITC staining identified uvarigrin-induced apoptosis in KBv200 and KB cells. These cells underwent incubation with DCFH-DA, or DiOC6, followed by flowcytometry for the measurement of reactive oxygen species (ROS) and mitochondrial membrane potential (deltapsim), respectively. The Western blotting analysis was performed on Caspase-9 activation.


RESULTS
Uvarigrin inhibited the growth of KBv200 cells and KB cells in vitro. Most of the uvarigrin-induced cells death was found to be due to apoptosis, as determined by Annexin V FITC staining. During the apoptosis, the level of ROS increased while the level of deltapsim decreased in a time-dependent manner. Uvarigrin triggered Caspase-9 activation.


CONCLUSIONS
Uvarigrin induced apoptosis in KBv200 cells and KB cells probably through a mitochondria-dependent pathway.
Li
Yan-fang
YF
Cancer Center, Sun Yat-Sen University, Guangzhou, China.
Liang
Yong-ju
YJ
Shi
Zhi
Z
Chen
Li-ming
LM
Ding
Yan
Y
Fu
Li-wu
LW
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053453:Caspase 9 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005663:Furans / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007624:KB Cells; D007783:Lactones / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000502:physiology; D010946:Plants, Medicinal / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism; D031046:Uvaria / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D005663:Furans; D007783:Lactones; D017382:Reactive Oxygen Species; C118092:uvarigrin; D053453:Caspase 9