title: The sequences of the spacer region between the atpF and atpA genes in the plastid genome allows discrimination among three varieties of medicinal Angelica.
abstract: The dried roots of Angelica acutiloba Kitagawa var. acutiloba Kitagawa, A. acutiloba Kitagawa var. iwatensis Hikino and A. acutiloba Kitagawa var. sugiyamae Hikino have been used as the herbal medicine known in Japan as Japanese Angelica Root. The respective morphological features of, in particular, A. acutiloba var. sugiyamae and A. acutiloba var. iwatensis are similar, and they are not easy to distinguish morphologically from each other. In an attempt to find a method for discriminating among these three varieties, we compared the nucleotide sequence of the spacer region between the atpF and atpA genes among the respective plastid genomes. Comparison of these sequences allowed us to identify each of the three varieties unequivocally.
authors.affiliation: Department of Nutritional Management, Faculty of Health Sciences, Hyogo University, Kakogawa, Hyogo, Japan. khosokaw@hyogo-dai.ac.jp
authors.forename: Keizo
authors.initials: K
authors.lastname: Hosokawa
authors.forename: Atsuyuki
authors.initials: A
authors.lastname: Hishida
authors.forename: Ikuo
authors.initials: I
authors.lastname: Nakamura
authors.forename: Toshiro
authors.initials: T
authors.lastname: Shibata
mesh_terms: D029969:Angelica / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D021901:DNA, Intergenic / Q000737:chemistry*; D018744:DNA, Plant / Q000737:chemistry*; D006801:Humans; D008517:Phytotherapy; D018515:Plant Leaves; D018087:Plastids / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D021901:DNA, Intergenic; D018744:DNA, Plant