title: Beneficial effect of erythropoietin administration on murine infection with Trypanosoma congolense.
abstract: The effect of erythropoietin treatment on Trypanosoma congolense infection in mice was studied. Survival rates of mice were dramatically improved by treatment with recombinant human erythropoietin (r-hu-EPO; 5,000 U/kg) when infected with 1,000 cells of T. congolense IL3000 (P < 0.05). All the untreated mice infected with T. congolense IL3000 died by day 9 of infection; however, 100%, 50%, and 25% of the mice treated with r-hu-EPO for 8 days survived to day 20, day 40, and day 60 of the parasitical infection, respectively. Anti-8-hydroxy-2'-deoxyguanosine antibody, a biomarker for oxidative damage of DNA, yielded positive reactions in the cytoplasm of the parasites recovered from the mice treated with r-hu-EPO. These results, taken together, indicate that erythropoietin administration is effective for the treatment of T. congolense infection.
authors.affiliation: Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yoshiko Yanagimoto
authors.initials: YY
authors.lastname: Ueta
authors.forename: Noboru
authors.initials: N
authors.lastname: Inoue
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Hideki
authors.initials: H
authors.lastname: Saitoh
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000740:Anemia / Q000517:prevention & control; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004921:Erythropoietin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D020869:Gene Expression Profiling; D006400:Hematocrit; D051379:Mice; D008810:Mice, Inbred C57BL; D018512:Parasitemia / Q000150:complications / Q000188:drug therapy* / Q000469:parasitology; D010419:Pentamidine / Q000494:pharmacology; D016133:Polymerase Chain Reaction / Q000379:methods; D017467:Receptors, Erythropoietin / Q000096:biosynthesis / Q000187:drug effects; D011994:Recombinant Proteins; D016019:Survival Analysis; D013997:Time Factors; D014348:Trypanosoma congolense / Q000187:drug effects* / Q000302:isolation & purification; D014353:Trypanosomiasis, African / Q000188:drug therapy* / Q000401:mortality / Q000469:parasitology
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D015342:DNA Probes; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins; D004921:Erythropoietin; D010419:Pentamidine