[Detection of 16S ribosomal RNA gene of bacteria in prostate tissues of adults].
OBJECTIVE
To investigate the role of bacteria in chronic prostatitis.


METHODS
Complete specimens of prostate were obtained from 140 organ donors, aged 20 - 35, at autopsy. A piece of tissue was collected from the peripheral zone of prostate from each specimen and was divided into 2 parts to undergo pathological examination and PCR so as to detect the 16S ribosomal RNA (16S rRNA) gene of bacteria.


RESULTS
Focal mild inflammation was shown in 46 of the 104 specimens (32.9%), including interstitial inflammation in 42 specimens, inflammation in both interstitial and body of gland in 3 specimens, and perigladulitis in 1 specimen. Twenty-seven of the 140 specimens (19.3%) were positive in 16S rRNA gene. The positive rate of 16S rRNA gene of the specimens with prostatitis was 48.9%, significantly higher than that of the specimens without prostatitis (5.3%, P < 0.001).


CONCLUSIONS
Bacteria may play an important role in the pathogenesis of chronic prostatitis.
Xie
Hui
H
Department of Urology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
Huang
Hui-cong
HC
Yang
Yi-rong
YR
Wu
Jian-bo
JB
He
Qiu-xiang
QX
Zhu
Qi-jian
QJ
Chen
Jian-ou
JO
Wang
Si-qi
SQ
Li
Cheng-di
CD
Xia
Peng
P
Shen
Long-jie
LJ
D000328:Adult; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000382:microbiology*; D011472:Prostatitis / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S