Involvement of nuclear factor-kappaB in the inhibition of pro-inflammatory mediators by pinosylvin.
Nuclear factor-kappaB (NF-kappaB) is a critical transcription factor for maximal expression of many of the cytokines that are involved in the pathogenesis of inflammatory diseases. In this study, we found that pinosylvin, a natural stilbenoid that is a component of the pine leaf (Pinus densiflora), significantly inhibited LPS-induced NF-kappaB activation in a concentration-dependent manner. Additionally, pinosylvin was found to inhibit the LPS-induced phosphorylation and degradation of IkappaB alpha in THP-1 cells. Therefore, we have attempted to determine whether pinosylvin can inhibit the expression of cytokines possessing NF-kappaB binding sites in their promoter regions. In a consistent result, pinosylvin inhibited LPS-induced production of TNF alpha and interleukin-8 (IL-8). Taken together, these results show that pinosylvin suppresses the production of pro-inflammatory mediators through the inhibition of the NF-kappaB signaling pathway.
Lee
Jongsung
J
Biospectrum Life Science Institute, Gunpo City, Gyunggi Do, Republic of Korea.
Jung
Eunsun
E
Lim
Jihee
J
Lee
Jiyoung
J
Hur
Sungran
S
Kim
Sang Suk
SS
Lim
Sangbin
S
Hyun
Chang-Gu
CG
Kim
Yeong Shik
YS
Park
Deokhoon
D
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001665:Binding Sites; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000235:genetics; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000235:genetics; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors; D008156:Luciferases / Q000032:analysis; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D010766:Phosphorylation; D028223:Pinus / Q000737:chemistry; D011401:Promoter Regions, Genetic; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000235:genetics; D000094162:Phytoalexins
D000893:Anti-Inflammatory Agents; D051546:Cyclooxygenase 2; D052246:Cyclooxygenase 2 Inhibitors; D016207:Cytokines; D020672:I-kappa B Proteins; D016209:Interleukin-8; D008070:Lipopolysaccharides; D008156:Luciferases; D016328:NF-kappa B; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; C049032:pinosylvin; D000094162:Phytoalexins