[Establishment of hepatic metastatic subline of nasopharyngeal carcinoma cell line].
OBJECTIVE
To establish a hepatic metastatic subline of nasopharyngeal carcinoma (NPC) cell line.


METHODS
NPC cells metastatic to the liver were isolated from nude mice and the invasion and metastatic ability of the cells was observed in vivo and in vitro.


CONCLUSIONS
The invasion and metastasis activity of 5-8F-H3B-EGFP (an in vivo isolate with enhanced liver metastatic behaviors) were enhanced obviously in comparison with the parent cell line 5-8F-EGFP. This subline may be useful for cloning genes related to liver metastasis of NPC.
Han
Chun
C
Department of Pathology, Southern Medical University, Guangzhou 510515, China. xiaochun@fimmu.com
Wang
Shuang
S
Liu
Li
L
Yao
Kai-tai
KT
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D004195:Disease Models, Animal; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007633:Keratins / Q000032:analysis; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation
D049452:Green Fluorescent Proteins; D007633:Keratins