A lectin with some unique characteristics from the samta tomato.
A lectin, with a molecular mass of 79 kDa, and with specificity toward rhamnose and O-nitrophenyl-beta-D-galactopyranoside, was isolated from samta tomato fruits. The procedure entailed ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose and gel filtration by fast protein liquid chromatography on Superdex 75. The lectin was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel and CM-cellulose. The lectin was stable up to 70 degrees C. The lectin activity was potentiated by NaOH solutions (25-100 mM), but was reduced by 50 and 100 mM HCl solutions. The activity of the lectin was reduced in the presence of CaCl(2), MgCl(2) and ZnCl(2), but was potentiated by 5 and 10 mM AlCl(3) solutions. The lectin stimulated the mitogenic response in mouse splenocytes and inhibited human immunodeficiency virus-1 reverse transcriptase with an IC(50) of 6.2 microM.
Wang
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Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China.
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D000818:Animals; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D020128:Inhibitory Concentration 50; D018551:Solanum lycopersicum / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D008934:Mitogens; D037121:Plant Lectins; D054303:HIV Reverse Transcriptase