title: RACK1, a novel hPER1-interacting protein.
abstract: PER1, an important component of circadian clock systems, plays a critical role in regulating the period length and maintaining the precision and stability of the period of circadian rhythms. RACK1 (receptor for activated protein kinase C-1), a member of the WD-40 family of proteins, can interact with numerous signaling proteins and is regarded as a scaffolding, anchor, or adaptor protein in multiple intracellular signal transduction pathways. In the present study, we identified and confirmed RACK1 as a novel protein interacting with human clock protein, hPER1, using the yeast two-hybrid system and co-immunoprecipitation experiment. Further study by RT-PCR showed that RACK1 was expressed widely in tissues and there was no obvious expressional rhythmicity. However, RNA interfering plasmid inhibiting hPER1 (pTER/hPER1-II) could not interfere expression of RACK1. These results together suggested that RACK1 might act as a novel signal molecule to mediate or regulate the functions of PER1 through protein interaction.
authors.affiliation: West China Medical Center, Sichuan University, Chengdu, Sichuan 610041, P. R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Hu
authors.forename: Fang
authors.initials: F
authors.lastname: Lu
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanyou
authors.initials: Y
authors.lastname: Liu
authors.forename: Desang
authors.initials: D
authors.lastname: Liu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Jiang
authors.forename: Chaomin
authors.initials: C
authors.lastname: Wan
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.forename: Lu
authors.initials: L
authors.lastname: Gan
authors.forename: Yueqi
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001683:Biological Clocks / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002940:Circadian Rhythm / Q000502:physiology; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007031:Hypothalamus / Q000166:cytology; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D056950:Period Circadian Proteins; D034622:RNA Interference; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D020798:Two-Hybrid System Techniques
chemical_list: D018797:Cell Cycle Proteins; D009363:Neoplasm Proteins; C539022:PER1 protein, human; D056950:Period Circadian Proteins; C419368:RACK1 protein, human; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins