title: N type Ca2+ channels and RIM scaffold protein covary at the presynaptic transmitter release face but are components of independent protein complexes.
abstract: Fast neurotransmitter release at presynaptic terminals occurs at specialized transmitter release sites where docked secretory vesicles are triggered to fuse with the membrane by the influx of Ca2+ ions that enter through local N type (CaV2.2) calcium channels. Thus, neurosecretion involves two key processes: the docking of vesicles at the transmitter release site, a process that involves the scaffold protein RIM (Rab3A interacting molecule) and its binding partner Munc-13, and the subsequent gating of vesicle fusion by activation of the Ca2+ channels. It is not known, however, whether the vesicle fusion complex with its attached Ca2+ channels and the vesicle docking complex are parts of a single multifunctional entity. The Ca2+ channel itself and RIM were used as markers for these two elements to address this question. We carried out immunostaining at the giant calyx-type synapse of the chick ciliary ganglion to localize the proteins at a native, undisturbed presynaptic nerve terminal. Quantitative immunostaining (intensity correlation analysis/intensity correlation quotient method) was used to test the relationship between these two proteins at the nerve terminal transmitter release face. The staining intensities for CaV2.2 and RIM covary strongly, consistent with the expectation that they are both components of the transmitter release sites. We then used immunoprecipitation to test if these proteins are also parts of a common molecular complex. However, precipitation of CaV2.2 failed to capture either RIM or Munc-13, a RIM binding partner. These findings indicate that although the vesicle fusion and the vesicle docking mechanisms coexist at the transmitter release face they are not parts of a common stable complex.
authors.affiliation: Cellular and Molecular Biology Division, MP14-320 Toronto Western Research Institute, University Health Network, 399 Bathurst Street, Toronto, Ontario, M5T 2S8 Canada.
authors.forename: R
authors.initials: R
authors.lastname: Khanna
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: T J
authors.initials: TJ
authors.lastname: Collins
authors.forename: E F
authors.initials: EF
authors.lastname: Stanley
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000032:analysis / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D020864:Calcium Channels, N-Type / Q000032:analysis / Q000378:metabolism*; D002642:Chick Embryo; D019204:GTP-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism*; D013574:Synaptosomes / Q000737:chemistry / Q000378:metabolism; D020826:rab3A GTP-Binding Protein / Q000032:analysis / Q000378:metabolism
chemical_list: D030161:Avian Proteins; C500963:Cacna1b protein, mouse; D020864:Calcium Channels, N-Type; D018377:Neurotransmitter Agents; D019204:GTP-Binding Proteins; D020826:rab3A GTP-Binding Protein