title: Biomolecular sensor based on fluorescence-labeled aptamer.
abstract: Fluorescent DNA probes for L-argininamide were developed by a combination of DNA aptamers and fluorophore-quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect L-argininamide specifically. The binding affinities were defined by the binding affinity of the original aptamer to indicate that the end labeling of the aptamer did not influence the affinities.
authors.affiliation: Department of Chemistry, Faculty of Engineering, Gunma University, Kiryu, Japan. ozaki@chem.gunma-u.ac.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ozaki
authors.forename: Akifumi
authors.initials: A
authors.lastname: Nishihira
authors.forename: Masayuki
authors.initials: M
authors.lastname: Wakabayashi
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sawai
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D052158:Aptamers, Peptide / Q000737:chemistry*; D001120:Arginine / Q000031:analogs & derivatives / Q000737:chemistry; D001483:Base Sequence; D015374:Biosensing Techniques; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004247:DNA / Q000737:chemistry; D015195:Drug Design; D005456:Fluorescent Dyes / Q000494:pharmacology; D008024:Ligands; D008956:Models, Chemical; D008969:Molecular Sequence Data; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
chemical_list: D052157:Aptamers, Nucleotide; D052158:Aptamers, Peptide; D005456:Fluorescent Dyes; D008024:Ligands; C069339:argininamide; D004247:DNA; D001120:Arginine