Increases in pig major acute-phase protein in wasting pigs brought to the abattoir.
Plasma proteins of wasting pigs were quantitatively and qualitatively compared with those of normal fattening pigs. Higher expression of a 120 kDa protein was observed in the plasma of wasting pigs by SDS-PAGE. This protein was identified as pig major acute-phase protein (Pig-MAP) by proteomic analysis using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The plasma concentration of Pig-MAP in wasting pigs was 7-fold higher than that of normal ones.
Yamane
Hiromi
H
Chiran Meat Inspection Center, Kagoshima Prefecture, Japan.
Kanouchi
Hiroaki
H
Arimizu
Goro
G
Obi
Takeshi
T
Oka
Tatsuzo
T
D000003:Abattoirs; D000209:Acute-Phase Proteins / Q000378:metabolism*; D000818:Animals; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D008460:Meat / Q000592:standards*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013552:Swine; D013553:Swine Diseases / Q000097:blood*; D019282:Wasting Syndrome / Q000097:blood / Q000662:veterinary*
D000209:Acute-Phase Proteins