title: [Preparation of polyclonal antibody against microcystin-LR].
abstract: Good quality polyclonal antibody against Microcystin-LR (MC-LR) was obtained from the New Zealand rabbit immunized with the homemade complete antigen BSA-MC-LR. Indirect ELISA shows the titer of this antibody is more than 1.5 x 10(5); Indirect competitive ELISA immobilized the coating antigen OVA-MC-LR was adapted to detect MCs, the calibration curve shows that the detection lower limit is 10 ng/L, and the range of quantitative detection was between 30 ng/L to 3 microg/L, with a good linearity, which will well potentially suit for sensitive analysis for MC-LR in drinking as well as surface water samples.
authors.affiliation: ESPC State Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Jian-wu
authors.initials: JW
authors.lastname: Sheng
authors.forename: Miao
authors.initials: M
authors.lastname: He
authors.forename: Han-chang
authors.initials: HC
authors.lastname: Shi
authors.forename: Yi
authors.initials: Y
authors.lastname: Qian
mesh_terms: D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis / Q000276:immunology*; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008387:Marine Toxins; D052998:Microcystins; D014873:Water Pollutants; C057862:cyanoginosin LR