In Planta production of immunogenic poliovirus peptide using tobacco mosaic virus-based vector system.
The tobacco mosaic virus (TMV) provides an attractive means of producing foreign peptides in plants. In this study, a TMV-based vector was designed such that a fragment encoding 15 amino acids of the poliovirus peptide (PVP) derived from the viral capsid proteins VP3 and VP1 of poliovirus type 1 Sabin was inserted downstream of the six-base 3' context nucleotide sequence of the TMV coat protein (CP) gene. This design allowed readthrough at the amber stop codon, thereby producing the chimeric TMV particle with both intact CP and CP-fusion protein (CP-PVP) in Nicotiana tabacum cv. Samsun infected with the TMV vector. The TMVCP-PVP virus particle induced antibodies against PVP as well as TMVCP in mice after intraperitoneal immunization. These data illustrate the potential of the readthrough translation system with TMVCP for antigen presentation and vaccine production.
Fujiyama
Kazuhito
K
The International Center for Biotechnology, Osaka University, Suita, Japan. fujiyama@icb.osaka-u.ac.jp
Saejung
Wanida
W
Yanagihara
Itaru
I
Nakado
Junko
J
Misaki
Ryo
R
Honda
Takeshi
T
Watanabe
Yuichiro
Y
Seki
Tatsuji
T
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D030821:Plants, Genetically Modified / Q000378:metabolism / Q000821:virology; D015202:Protein Engineering / Q000379:methods; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014162:Transfection / Q000379:methods
D036022:Capsid Proteins; C442540:VP1 protein, Poliovirus; C442541:VP2 protein, Poliovirus