Measurement of regional choroidal blood flow in rabbits and monkeys using fluorescent microspheres.
OBJECTIVE
To develop a quantitative measure of regional variation in choroidal blood flow (ChBF).


METHODS
Five million 15-microm fluorescent microspheres were injected into the left ventricles of 4 rabbits and 3 monkeys. The fixed globes were bleached, flat mounted, and photomicrographed. After image analysis to locate each microsphere, regional densities and blood flow were determined.


RESULTS
Regional variation in ChBF was clearly evident. In the rabbit, a high density of spheres was seen in the visual streak. This was surrounded by a middle peripheral area of low sphere density and a far peripheral region of moderately high density. In the monkeys, sphere density was markedly greater in the macula compared with the periphery. Contour plots produced lines of constant flow that were oval and extended farther nasally than temporally in the monkeys. The ratio of central to peripheral ChBF was much greater in the monkeys than in the rabbits.


CONCLUSIONS
Quantitative assessment of regional ChBF can be performed using a modification of the fluorescent microsphere impaction method.


CONCLUSIONS
This method of determining regional ChBF will be useful for studying the vascular effects of pharmacologic agents and for characterizing animal models of human disease involving the outer retina.
Nork
T Michael
TM
Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison 53792-3220, USA. tmnork@wisc.edu
Kim
Charlene B Y
CB
Shanmuganayagam
Dhanansayan
D
Van Lysel
Michael S
MS
Ver Hoeve
James N
JN
Folts
John D
JD
D000818:Animals; D001783:Blood Flow Velocity; D002829:Choroid / Q000098:blood supply*; D005456:Fluorescent Dyes; D018056:Hemorheology / Q000379:methods*; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008856:Microscopy, Fluorescence; D008863:Microspheres; D011817:Rabbits; D012039:Regional Blood Flow / Q000502:physiology
D005456:Fluorescent Dyes