Molecular and functional characterization of bovine beta-defensin-1.
We report the biochemical and functional properties of a novel bovine beta-defensin (bBD-1). Cloned from bovine mammary papillary duct epithelia, the bBD-1 cDNA predicts a 69 amino acid propeptide that is much more similar to human beta-defensin-1 (hBD-1) than to other bovine defensins. The bBD-1 gene contains two exons and one 8.5 kb intron. Using RT-PCR, we detected the bBD-1 transcript in the teat mucosa, kidney, vagina, ovary, oviduct, and colon. A synthetic bBD-1 peptide demonstrates potent antibacterial activity against Escherichia coli. The widespread expression of bBD-1 mRNA indicates that bBD-1 may play an important role in the bovine host defense against infections.
Aono
Shelly
S
Department of Anatomy, Physiology & Pharmacology, Auburn University, Auburn, AL 34849, USA.
Li
Changqing
C
Zhang
Guolong
G
Kemppainen
Robert J
RJ
Gard
Julie
J
Lu
Wuyuan
W
Hu
Xueyou
X
Schwartz
Dean D
DD
Morrison
Edward E
EE
Dykstra
Christine
C
Shi
Jishu
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D023083:beta-Defensins / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology
D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; D023083:beta-Defensins