title: Visualization of human sperm chromosomes by using mouse oocytes cryothawed after enucleation.
abstract: OBJECTIVE
  To evaluate whether previously enucleated mouse oocytes that are vitrified are usable for the analysis of human sperm chromosomes.
  
  
  METHODS
  Prospective, comparative laboratory study.
  
  
  METHODS
  Animal breeding and reproduction department in a rural Japan university.
  
  
  METHODS
  Sperm samples were obtained from a fertile donor.
  
  
  METHODS
  Human sperm were injected into previously enucleated-vitrified mouse oocytes.
  
  
  METHODS
  The rates of appearance of the human sperm chromosomes that were injected into previously enucleated-vitrified mouse oocytes.
  
  
  RESULTS
  The rates of appearance of human sperm chromosomes were 71%-72% in enucleated-vitrified mouse oocytes.
  
  
  CONCLUSIONS
  The use of enucleated-vitrified mouse oocytes allowed visualization of human sperm chromosomes.
authors.affiliation: Science of Plant and Animal Production, United Graduated School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan. yaaraki@nifty.com
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Araki
authors.forename: Midori
authors.initials: M
authors.lastname: Yoshizawa
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Araki
mesh_terms: D000818:Animals; D002467:Cell Nucleus; D002470:Cell Survival; D002478:Cells, Cultured; D002877:Chromosomes, Human; D015925:Cryopreservation; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D009865:Oocytes / Q000502:physiology; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000502:physiology*; D013396:Suction