[Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer].
OBJECTIVE
To study the feasibility of vector-mediated RNA interference for HER-2-positive breast cancer therapy.


METHODS
A plasmid vector capable of mediating HER-2 RNA interference was constructed, and HER-2-positive breast cancer cell line SKBR-3 was transfected with this constructed vector. The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting, and the growth and apoptosis of SKBR-3 cells was analyzed after transfection.


RESULTS
The expressions of HER-2 mRNA and HER-2 protein was downregulated in response to vector-mediated HER-2 RNA interference, which also resulted in tumor cell growth inhibition and increased number apoptotic cells.


CONCLUSIONS
HER-2 is a good target for RNA interference and RNA interference targeting HER-2 can lead to HER-2 breast cancer cell apoptosis and growth inhibition.
Hu
Xiao-qu
XQ
Center of Breast Cancer, Second Hospital of Sun Yat-sen University, Guangzhou 510272, China.
Qin
Li
L
Su
Feng-xi
FX
Yao
He-rui
HR
Chen
Ji-sheng
JS
Gong
Chang
C
Guo
Ju-jiang
JJ
Yu
Feng-yan
FY
Jia
Hai-xia
HX
D017209:Apoptosis; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases