title: Manganese superoxide dismutase based phylogeny of pathogenic fungi.
abstract: Superoxide dismutases (SODs), which provide protection against oxidative stress, exhibit an essential role for fungal cell survival, especially during host invasion. Here, 20 partial SOD sequences from 19 pathogenic fungi were determined and aligned with 43 homologous fungal sequences from databases. All sequences encoded tetrameric manganese (Mn)-containing SODs showing predicted cytosolic or mitochondrial subcellular localization. Numerous fungi possessed both cytosolic and mitochondrial MnSODs in addition to the mainly cytosolic copper/zinc isozyme. Moreover, MnSOD sequence variability was higher than SSU rRNA and similar to ITS rRNA, suggesting MnSOD could be used to identify closely related fungal species. MnSOD- and SSU rRNA-based phylogenetic trees were constructed and compared. Despite a more complex topology of the MnSOD tree, due to several gene duplication events, all the classic fungal classes and orders were recovered. A salient point was the existence of two paralogous cytosolic and mitochondrial MnSODs in some Ascomycota. A hypothetical evolutionary scenario with an early gene duplication of the "mitochondrial" gene is proposed.
authors.affiliation: ECOPA (EA 3609-Lille-2 University), IFR17, Lille Pasteur Institute, 59019 Lille cedex, France. emilie.frealle@pasteur-lille.fr
authors.forename: Emilie
authors.initials: E
authors.lastname: Fréalle
authors.forename: Christophe
authors.initials: C
authors.lastname: Noël
authors.forename: Nicole
authors.initials: N
authors.lastname: Nolard
authors.forename: Françoise
authors.initials: F
authors.lastname: Symoens
authors.forename: Maria-Sueli
authors.initials: MS
authors.lastname: Felipe
authors.forename: Eduardo
authors.initials: E
authors.lastname: Dei-Cas
authors.forename: Daniel
authors.initials: D
authors.lastname: Camus
authors.forename: Eric
authors.initials: E
authors.lastname: Viscogliosi
authors.forename: Laurence
authors.initials: L
authors.lastname: Delhaes
mesh_terms: D001203:Ascomycota / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity; D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D005658:Fungi / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D008957:Models, Genetic; D010802:Phylogeny; D012335:RNA, Ribosomal; D013482:Superoxide Dismutase / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D012335:RNA, Ribosomal; D013482:Superoxide Dismutase