Biosynthesis and bioactivity of trichosanthin in cultured crown gall tissues of Trichosanthes kirilowii Maximowicz.
Trichosanthin (TCS) from Trichosanthes kirilowii Maximowicz (T. kirilowii) can be used to treat choriocarcinoma. In this work, we established a novel system to produce TCS in crown gall tissues of T. kirilowii infected by Agrobacterium tumefaciens C58 (A. tumefaciens). In the crown gall tissues, a nopaline synthase (NOS) gene of A. tumefaciens was identified by polymerase chain reaction (PCR), and nopaline accumulation was confirmed by a high-voltage filter paper electrophoresis. Furthermore, we optimized conditions to culture the crown gall tissues able to grow fast and produce TCS in an auxin-free medium, and found that a fungal elicitor of Armillaria mellea was capable of stimulation of TCS secretion into the medium. Moreover, we identified that the TCS purified from the crown gall tissues could induce gastric cancer cell death. These data underscore the usefulness of our system as an inexpensive and virtually unlimited source of TCS.
Lei
Hetian
H
Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, Hai-Dian District, Beijing, 100094, PR China. Leihetian18@hotmail.com
Qi
Jianjun
J
Song
Jingyuan
J
Yang
Dejun
D
Wang
Yanzhi
Y
Zhang
Yinling
Y
Yang
Junshan
J
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000594:Amino Acid Oxidoreductases / Q000235:genetics; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000627:therapeutic use*; D001487:Basidiomycota / Q000235:genetics; D019149:Bioreactors; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D010941:Plant Tumors / Q000235:genetics* / Q000382:microbiology; D029778:Trichosanthes / Q000254:growth & development / Q000378:metabolism*; D015978:Trichosanthin / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D015978:Trichosanthin; D000594:Amino Acid Oxidoreductases; C021883:nopaline synthase