[Experimental study of effect of tanshinone on artery restenosis in rat carotid injury model].
OBJECTIVE
To observe the preventive and therapeutic effect of tanshinone (TA) on artery restenosis in the rat carotid injury model and explor the mechanism.


METHODS
Male SD rats were randomly divided into model control group, and low dose, moderate dose and high dose TA groups. Each group had 10 rats. The rats in the high, moderate and low dose groups were respectively fed with TA 120, 40,13.3 mg x kg(-1) x d(-1) by gast rogavage; the rats in the model control group were fed with the same volume solvent. Two days later, the rat's right carotid artery was injuried by balloon dilatation to induce intimal thickening for establishing the restenosis model. After 2 weeks of treatment, the artery was harvested and stained by hematoxylin-elsin (HE) and immunohistochemistry of PCNA, NF-kappaB and iNOS. The morphological changes were checked under microscope. The area of the intimal and medial layer of the vessels, and their ratios were analyzed with image analysis software. The expression level of PCNA, NF-kappaB and iNOS were used as the positive index.


RESULTS
The intimal area and intima-to-media ratio of the injuried artery increased obviously, suggesting the model was successful. Compared with the model group, TA significantly decreased the intimal area and intima-to-media ratio (P < 0.05), and also decreased the positive index of PCNA and the positive ratio of NF-kappaB and iNOS (P < 0.05).


CONCLUSIONS
TA can effectively inhibit intimal thickening and inflammation. This result suggestes that TA may play a positive role in the prevention of restenosis after PTCA.
Li
Xin
X
School of Huaxi Pharmacy, Sichuan University, Chengdu 610041, China.
Du
Jun-Rong
JR
Wang
Wei-Dong
WD
Zheng
Xiao-Yuan
XY
Sun
Wei
W
Zong
Xu
X
Zheng
Hu
H
Qian
Zhong-Ming
ZM
D045784:Abietanes; D015906:Angioplasty, Balloon, Coronary / Q000009:adverse effects; D000818:Animals; D020212:Carotid Artery Injuries / Q000150:complications*; D017536:Carotid Artery, Common / Q000378:metabolism / Q000473:pathology; D016893:Carotid Stenosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry; D017539:Tunica Intima / Q000378:metabolism / Q000473:pathology*
D045784:Abietanes; D016328:NF-kappa B; D010616:Phenanthrenes; D018809:Proliferating Cell Nuclear Antigen; C021751:tanshinone; D052247:Nitric Oxide Synthase Type II