title: Delivery of condensed DNA by liposomal non-viral gene delivery system into nucleus of dendritic cells.
abstract: In this study, we developed novel double-membranous non-viral gene delivery system modified with SV-40 T antigen-derived nuclear localization signal (NLS-DMEND) for delivery of luciferase plasmid DNA to nucleus of non-dividing mouse bone marrow-derived dendritic cells (BMDC). Intracellular trafficking and gene expression of NLS-DMEND in the BMDC were evaluated. Condensed DNA was observed in the nucleus by confocal laser scanning microscopy, and the NLS-DMEND induced significant luciferase activity in the BMDC. It was suggested that the condensed DNA particle transferred into nucleus via energy dependent manner, since the nuclear transfer was inhibited by metabolic inhibitors. In conclusion, condensed plasmid DNA was delivered into the nucleus of non-dividing BMDC by NLS-DMEND.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Rumiko
authors.initials: R
authors.lastname: Moriguchi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kogure
authors.forename: Arisa
authors.initials: A
authors.lastname: Minoura
authors.forename: Tomoya
authors.initials: T
authors.lastname: Masuda
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Akita
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kato
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hamada
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ueno
authors.forename: Shiroh
authors.initials: S
authors.lastname: Futaki
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D003713:Dendritic Cells / Q000378:metabolism*; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes; D008156:Luciferases / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D019913:Nuclear Localization Signals; D010957:Plasmids
chemical_list: D008081:Liposomes; D019913:Nuclear Localization Signals; D004247:DNA; D008156:Luciferases