Improved production of alkaline protease from a mutant of alkalophilic Bacillus pantotheneticus using molasses as a substrate.
An alkalophilic bacterial isolate identified as Bacillus pantotheneticus, isolated from saline-alkali soils of Avadh region of UP, India, was studied for the production of alkaline protease. The mutant of the isolated species showed 44% improved production over the parent strain. Organic nitrogen sources supported better protease production than the inorganic sources. The production of alkaline protease was (242 U/ml) in the medium containing molasses, which was comparable with molasses and wheat bran (285 U/ml) as carbon and nitrogen sources, respectively. Protease production was best at pH 10 and temperature 30 degrees C. The Km (for casein) was 11 mg/ml and Vmax was 380-microg tyrosine/ml/min. The enzyme was stable between pH 7 and 10.7 and temperature between 30 and 60 degrees C with a pH and temperature optimum at 8.4 and 40 degrees C, respectively. The results indicated that molasses was an optimal substrate for alkaline protease production.
Shikha
Department of Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad 224 001, UP, India. dr_shikha2003@yahoo.co.in
Sharan
Adhyayan
A
Darmwal
Nandan S
NS
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000378:metabolism; D010450:Endopeptidases / Q000096:biosynthesis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008965:Molasses; D009154:Mutation; D013379:Substrate Specificity; D013696:Temperature
D001426:Bacterial Proteins; D010450:Endopeptidases; C087822:alkaline protease