[Preparation of amylopectin modified dipyridamole liposome and its tissue distribution in mice].
OBJECTIVE
To prepare amylopectin anchored dipyridamole (DIP) liposome and to study its tissue distribution in mice.


METHODS
The regular DIP liposomes were prepared by film-scatter method. The amphiphilic O-palmitoyl amylopectin was synthesized and added to modify the surface of liposome. The entrapping efficiency, zeta potential, mean diameter, span of modified and regular liposomes were assayed. The RP-HPLC was used for the determination of DIP concentration in mice tissue.


RESULTS
After modification, the entrapping efficiency depressed, zeta potential was raised, mean diameter and span had no obvious change. The level of DIP in lung, liver and spleen for regular liposomes were higher than that of injections. Compared with regular liposomes, the modified liposomes increased the DIP level in lung, and decreased the DIP level in liver, spleen, moreover, lengthened the retention time of DIP in lung.


CONCLUSIONS
The distribution of modified liposome in mice was markedly changed as compared with regular liposomes and injections. The modified liposomes had obvious lung targeting property.
Cheng
Ji
J
Pharmaceutical Research Institute, China Pharmaceutical University, Nanjing. chengji45@hotmail.com
Zhu
Jia-bi
JB
Yang
Si-xing
SX
Wang
Chang-bin
CB
D000687:Amylopectin / Q000031:analogs & derivatives* / Q000737:chemistry; D000818:Animals; D019540:Area Under Curve; D004176:Dipyridamole / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D008081:Liposomes; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D010168:Palmitates / Q000737:chemistry*; D010316:Particle Size; D014018:Tissue Distribution
D008081:Liposomes; C514132:O-palmitoyl amylopectin; D010168:Palmitates; D004176:Dipyridamole; D000687:Amylopectin