title: [Protein-protein interaction between ESAT-6 and CFP-10 of mycobacterium tuberculosis].
abstract: OBJECTIVE
  To investigate the protein-protein interaction between ESAT-6 and CFP-10 of mycobacterium tuberculosis.
  
  
  METHODS
  ESAT-6 gene and CFP-10 gene were amplified from mycobacterium tuberculosis genome DNA. The ESAT-6 gene was subcloned into pGBKT7 and the CFP-10 gene was subcloned into pGADT7-Rec. After being verified with restriction endonuclease digestion and DNA sequencing, the recombinant vectors were transformed into yeast cell AH109 by lithium acetate method.
  
  
  RESULTS
  The yeast cells co-transformed with pGBKT7-ESAT-6 and pGADT7-CFP-10 grew on SD/-Ade/-His/-Leu/-Trp plates, and beta-galactosidase activity assays showed positive results.
  
  
  CONCLUSIONS
  ESAT-6 and CFP-10 protein could interact with each other in yeast cells.
authors.affiliation: Research Unit of Infection and Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Lang
authors.initials: L
authors.lastname: Bao
authors.forename: Hui-dong
authors.initials: HD
authors.lastname: Zhang
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Hai-long
authors.initials: HL
authors.lastname: Zhu
authors.forename: Ya-sha
authors.initials: YS
authors.lastname: Li
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020798:Two-Hybrid System Techniques
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; D004269:DNA, Bacterial; C093276:ESAT-6 protein, Mycobacterium tuberculosis