title: Measurement of plasma renin activity.
abstract: Measurement of angiotensin 1, released in plasma by the action of endogenous enzyme renin on endogenous substrate, angiotensinogen is described. Details are given for the generation of angiotensin 1 from plasma using controlled conditions of pH and temperature. A radioimmunoassay to quantify the generated material is then described using anti-angiotensin 1 antibody and iodinated angiotensin 1 as label. Separation of the antibody-bound fraction from the free is achieved using dextran-coated charcoal. Problems of cryoactivation of prorenin and the labile nature of angiotensin 1 are highlighted. Additional notes describe steps in the assay that are critical.
authors.affiliation: Department of Clinical Biochemistry and Immunology, Leeds General Infirmary, UK.
authors.forename: Marion L
authors.initials: ML
authors.lastname: Cawood
mesh_terms: D000803:Angiotensin I / Q000032:analysis / Q000097:blood; D000818:Animals; D004789:Enzyme Activation; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D012083:Renin / Q000032:analysis* / Q000097:blood*; D013379:Substrate Specificity
chemical_list: D000803:Angiotensin I; D012083:Renin