Dissociation between vascular oxidative stress and cardiovascular function in Wistar Kyoto and spontaneously hypertensive rats.
It has not been completely demonstrated if hypertension may, in part, develop as a result of increased oxidative stress (OS), inflammation and little is known about the short-term effects of antioxidant therapy. This study was designed to appreciate the effect of 7 days vitamin C-enriched diet (5 g/kg/day) on hemodynamic function and vascular OS in normotensive Wistar Kyoto rats and hypertensive rats (SHR). Aorta NAD(P)H oxidase activity was determinate and free radicals evaluated by electron spin resonance with a spin probe CP-H. Matrix metalloproteinase-1 (MMP-1) and monocyte chemoattractant protein-1 (MCP-1) expression were measured. The treatment with vitamin C did not change arterial pressure in SHR but prevented the increase in OS levels in SHR aortas. MMP-1 and MCP-1 expressions were more intense in the media of SHR aortas than in those of WKY rats but these expressions were not modified by vitamin C-pretreatment. Vitamin C-pretreatment was not able to protect heart against in vitro ischemia-reperfusion dysfunctions. These data may suggest that treatment with high doses of vitamin C in SHR can limit over-production of reactive oxygen species; however this effect was not accompanied with changes in arterial pressure and protection against I-R dysfunctions. Dissociation between vascular oxidative stress and cardiovascular function may be evoked.
Sicard
Pierre
P
Laboratoire de Physiopathologie et Pharmacologie Cardio-vasculaires Expérimentales, IFR no. 100, Facultés de Médecine et de Pharmacie, 7, Boulevard Jeanne d'Arc, 21000 Dijon, France. sicard123@yahoo.fr
Oudot
Alexandra
A
Guilland
Jean-Claude
JC
Moreau
Daniel
D
Vergely
Catherine
C
Rochette
Luc
L
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001011:Aorta / Q000378:metabolism; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D018932:Chemokine CCL2 / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D006973:Hypertension / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D009924:Organ Culture Techniques; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D015427:Reperfusion Injury / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D019255:NADPH Oxidases; D020781:Matrix Metalloproteinase 1; D001205:Ascorbic Acid