Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein.
Two recombinant antigens which individually protect mice from lethal intranasal infection were studied in combination, either as a mixture of two separately expressed proteins or as a single chimeric expression product. Mice vaccinated with either combination survived longer than mice given single antigens. Immunized mice also exhibited specific IgG immunoglobulins and yielded splenocytes which produced interferon-gamma in response to either antigen. The chimeric antigen has the practical advantage of offering enhanced protection from multiple components without increasing production costs.
Shubitz
Lisa F
LF
The Valley Fever Center for Excellence, University of Arizona, Tucson, AZ 85721, USA. lfshubit@u.arizona.edu
Yu
Jieh-Juen
JJ
Hung
Chiung-Yu
CY
Kirkland
Theo N
TN
Peng
Tao
T
Perrill
Robert
R
Simons
Julie
J
Xue
Jianmin
J
Herr
Roger A
RA
Cole
Garry T
GT
Galgiani
John N
JN
D000595:Amino Acid Sequence; D000818:Animals; D003045:Coccidioides; D003047:Coccidioidomycosis / Q000382:microbiology / Q000517:prevention & control*; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000627:therapeutic use*; D005657:Fungal Vaccines / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000517:prevention & control*; D022223:Vaccines, Subunit / Q000627:therapeutic use*; D014614:Vaccines, Synthetic / Q000627:therapeutic use
D005656:Fungal Proteins; D005657:Fungal Vaccines; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic