[Raising fat content in transgenic rice by anti-PEP gene transformation].
Gene for phosphoenolpyruvate carboxylase (PEPCase) was incorporated in antisense orientation into genome of japonica rice variety Xiushui 11 through Agrobacterium tumefaciens-mediated transformation of mature seeds-derived embryogenic calli, and a total of 95 transgenic plants were regenerated, which were confirmed by histochemical detection of GUS activity (Fig.2), PCR assay (Fig.3) and Southern hybridization analysis (Fig.4). The transgene was inherited by the T1 progeny and Mendelian 3:1 segregation ratio was observed in most transgenic lines. It was found that the fat content of hulled grains of T2 transgenic lines was 0.37 +/- 0.12 per cent higher than that of the control, most of which were statistically very significant (Table 2).
Wu
Guan-Ting
GT
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Lang
Chun-Xiu
CX
Hu
Zhang-Hua
ZH
Chen
Xiao-Yun
XY
Wang
Fu-Lin
FL
Jin
Wei
W
Chen
Jin-Qing
JQ
D016960:Agrobacterium tumefaciens / Q000235:genetics; D015139:Blotting, Southern; D016373:DNA, Antisense / Q000235:genetics*; D005223:Fats / Q000378:metabolism*; D018745:Genome, Plant / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
D016373:DNA, Antisense; D005223:Fats; D010730:Phosphoenolpyruvate Carboxylase