title: Changes of the cortex proteome and Apolipoprotein E in transgenic mouse models of Alzheimer's Disease.
abstract: Transgenic mice carrying human Amyloid Precursor Protein mutations present amyloid plaque deposition in the brain upon aging. In this study, we characterized the changes of cortex proteome and endogenous Apolipoprotein E in these mice. Differential analysis of two-dimensional electrophoresis images revealed spots altered upon aging, transgene addition and plaque deposition. Alpha-synuclein and cytochrome oxidase polypeptide Va were up-regulated in transgenic mice. Upon aging, expression of ATP synthase alpha, alpha enolase, UMP-CMP kinase, and dihydropyrimidinase like-2 protein was modified. These proteins and their modification probably play a role in the amyloid aggregate formation in these mice.
authors.affiliation: Neuroproteomics Group, Department of Structural Biology and Bioinformatics, Geneva University, Faculty of Medicine, Geneva, Switzerland. Odile.Carrette@medecine.unige.ch
authors.forename: O
authors.initials: O
authors.lastname: Carrette
authors.forename: J A
authors.initials: JA
authors.lastname: Burgess
authors.forename: P R
authors.initials: PR
authors.lastname: Burkhard
authors.forename: C
authors.initials: C
authors.lastname: Lang
authors.forename: M
authors.initials: M
authors.lastname: Côte
authors.forename: N
authors.initials: N
authors.lastname: Rodrigo
authors.forename: D F
authors.initials: DF
authors.lastname: Hochstrasser
authors.forename: J-C
authors.initials: JC
authors.lastname: Sanchez
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001057:Apolipoproteins E / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D020543:Proteome
chemical_list: D001057:Apolipoproteins E; D020543:Proteome