title: Synthesis and single enzyme activity of a clicked lipase-BSA hetero-dimer.
abstract: Click chemistry is used to construct a novel lipase-BSA hetero-dimer, in which the latter protein acts as a foot enabling the anchoring of the enzyme onto the surface for single enzyme studies.
authors.affiliation: Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands.
authors.forename: Nikos S
authors.initials: NS
authors.lastname: Hatzakis
authors.forename: Hans
authors.initials: H
authors.lastname: Engelkamp
authors.forename: Kelly
authors.initials: K
authors.lastname: Velonia
authors.forename: Johan
authors.initials: J
authors.lastname: Hofkens
authors.forename: Peter C M
authors.initials: PC
authors.lastname: Christianen
authors.forename: Allan
authors.initials: A
authors.lastname: Svendsen
authors.forename: Shamkantr A
authors.initials: SA
authors.lastname: Patkar
authors.forename: Jesper
authors.initials: J
authors.lastname: Vind
authors.forename: Jan C
authors.initials: JC
authors.lastname: Maan
authors.forename: Alan E
authors.initials: AE
authors.lastname: Rowan
authors.forename: Roeland J M
authors.initials: RJ
authors.lastname: Nolte
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000201:enzymology; D002417:Cattle; D004796:Clinical Enzyme Tests; D019281:Dimerization; D004800:Enzymes, Immobilized / Q000737:chemistry; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D012710:Serum Albumin, Bovine; D008049:Lipase