title: Synthesis and in vitro cytotoxicity of haloderivatives of noscapine.
abstract: Three haloderivatives of noscapine 2-4 were synthesized chemoselectively and their in vitro cytotoxicity was assessed by MTT assay on U-87 human glioblastoma cell lines. At 50 microM concentration after 72 h, 9-chloronoscapine 2, 9-bromonoscapine 3 (EM011), and 9-iodonoscapine 4 killed 87.8%, 51.2%, and 56.8% cells, respectively, however noscapine kills only 40% of the cells; revealing 9-chloronoscapine as a potential cytotoxic agent than noscapine and 9-bromonoscapine (EM011). At low concentration (1 microM) 9-bromonoscapine (46.7%) and 9-chloronoscapine (45.7%) did not show any significant difference.
authors.affiliation: Synthetic Organic Chemistry Research Laboratory, Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110007, India. akhilesh682000@yahoo.com
authors.forename: Akhilesh Kumar
authors.initials: AK
authors.lastname: Verma
authors.forename: Sandhya
authors.initials: S
authors.lastname: Bansal
authors.forename: Jaspal
authors.initials: J
authors.lastname: Singh
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Tiwari
authors.forename: V
authors.initials: V
authors.lastname: Kasi Sankar
authors.forename: Vibha
authors.initials: V
authors.lastname: Tandon
authors.forename: Ramesh
authors.initials: R
authors.lastname: Chandra
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005909:Glioblastoma / Q000188:drug therapy / Q000473:pathology; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D009665:Noscapine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; D013844:Thiazoles; D009665:Noscapine; C022616:thiazolyl blue