title: The entrapment of corrosion products from CoCr implant alloys in the deposits of calcium phosphate: a comparison of serum, synovial fluid, albumin, EDTA, and water.
abstract: Physical wear of orthopedic implants is inevitable. CoCr alloy samples, typically used in joint reconstruction, corrode rapidly after removal of the protective oxide layer. The behavior of CoCr pellets immersed in human serum, foetal bovine serum (FBS), synovial fluid, albumin in phosphate-buffered saline (PBS), EDTA in PBS, and water were studied using X-ray Photoelectron Spectroscopy (XPS) and Time-of-Flight Secondary Ion Mass Spectroscopy (ToF-SIMS). The difference in the corrosive nature of human serum, water, albumin in PBS and synovial fluid after 5 days of immersion was highlighted by the oxide layer, which was respectively 15, 3.5, 1.5, and 1.5 nm thick. The thickness of an additional calcium phosphate deposit from human serum and synovial fluid was 40 and 2 nm, respectively. Co and Cr ions migrated from the bulk metal surface and were trapped in this deposit by the phosphate anion. This may account for the composition of wear debris from CoCr orthopedic implants, which is known to consist predominantly of hydroxy-phosphate compounds. Known components of synovial fluid including proteoglycans, pyrophosphates, phospholipids, lubricin, and superficial zone protein (SZP), have been identified as possible causes for the lack of significant calcium phosphate deposition in this environment. Circulation of these compounds around the whole implant may inhibit calcium phosphate deposition.
authors.affiliation: Interface Analysis Centre, University of Bristol, 121 St. Michael's Hill, Bristol, BS2 8BS, UK. andrewclewis@postmaster.co.uk
authors.forename: A C
authors.initials: AC
authors.lastname: Lewis
authors.forename: M R
authors.initials: MR
authors.lastname: Kilburn
authors.forename: P J
authors.initials: PJ
authors.lastname: Heard
authors.forename: T B
authors.initials: TB
authors.lastname: Scott
authors.forename: K R
authors.initials: KR
authors.lastname: Hallam
authors.forename: G C
authors.initials: GC
authors.lastname: Allen
authors.forename: I D
authors.initials: ID
authors.lastname: Learmonth
mesh_terms: D000418:Albumins / Q000737:chemistry; D000818:Animals; D002021:Buffers; D002130:Calcium Phosphates / Q000737:chemistry*; D002417:Cattle; D002858:Chromium Alloys / Q000737:chemistry*; D003343:Corrosion; D004492:Edetic Acid / Q000737:chemistry; D005312:Fetal Blood / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D011475:Prosthesis Failure; D012965:Sodium Chloride; D018629:Spectrometry, Mass, Secondary Ion; D013052:Spectrometry, X-Ray Emission; D013314:Stress, Mechanical; D013582:Synovial Fluid / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000418:Albumins; D002021:Buffers; D002130:Calcium Phosphates; D002858:Chromium Alloys; D014867:Water; D012965:Sodium Chloride; C020243:calcium phosphate; D004492:Edetic Acid