[Effect of hypericum perforatum on preventing acute injury of rat pulmonary microvascular endothelial cells induced by paraquat].
OBJECTIVE
To examine the effect of hypericum perforatum (HP) on preventing the acute injury of rat pulmonary microvascular endothelial cells (rPMVECs) induced by paraquat (PQ) and the change of production of reactive oxygen species (ROS).


METHODS
rPMVECs was cultured and divided into three groups: PQ group, HP group and blank control group. Paraquat (0.1 mmol/L) was added to cells in the PQ group and the HP group. Half hour later, hypericum perforatum was added to cells in the HP group, with a concentration of 250 mg/L, 10 mg/L, or 1 mg/L. The changes of growth inhibition rates were monitored by MTT at an interval of 3, 12, 24, 48, and 72 hours. Meanwhile, the total activity of SOD and volume of MDA in the supernatant were detected by spectrophotometry.


RESULTS
With the increased concentration and length of existence of hypericum perforatum, the growth inhibit rates decreased accordingly. Hypericum perforatum also inhibited the depressing of the total activity of SOD(P<0.05). The MDA was reduced after 72 hours (P<0.05).


CONCLUSIONS
Hypericum perforatum can protect the rPMVECs from acute injury induced by paraquat.
Wei
Wei
W
Department of Emergency, West China Hospital, Sichuan University, Chengdu 610041, China.
He
Qing
Q
D000818:Animals; D002196:Capillaries / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology*; D020902:Hypericum / Q000737:chemistry*; D008168:Lung / Q000098:blood supply*; D008297:Male; D010269:Paraquat; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D010269:Paraquat