title: Phylogenetic correlation of Greek and Italian orf virus isolates based on VIR gene.
abstract: Thirteen orf virus isolates obtained during the time period between 1995 and 2004 from crusted scab lesions of nine sheep and four goats from different geographical areas of Greece and Italy with suspected contagious ecthyma infection were analyzed. DNA of all isolates was successfully amplified by PCR with the primers 045F-045R and identified them as parapox virus. Partial DNA sequence of orf virus interferon resistant (VIR) gene, phylogenetic analysis of the available isolates and amino acid comparison of the interferon resistance protein encoded by this genomic region was carried out. According to the results of the present report a precise characterisation of the genomic region studied might provide evidence for the genetic variation and movement of the circulating orf virus strains.
authors.affiliation: Centre of Athens Veterinary Institutes, Institute of Infectious and Parasitic Diseases, 25 Neapoleos Street, 15310 Agia Paraskevi, Attiki, Greece.
authors.forename: Christine
authors.initials: C
authors.lastname: Kottaridi
authors.forename: Kyriaki
authors.initials: K
authors.lastname: Nomikou
authors.forename: Liana
authors.initials: L
authors.lastname: Teodori
authors.forename: Giovanni
authors.initials: G
authors.lastname: Savini
authors.forename: Rossella
authors.initials: R
authors.lastname: Lelli
authors.forename: Panayotis
authors.initials: P
authors.lastname: Markoulatos
authors.forename: Olga
authors.initials: O
authors.lastname: Mangana
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004279:DNA, Viral / Q000737:chemistry*; D004474:Ecthyma, Contagious / Q000175:diagnosis / Q000821:virology*; D015511:Goat Diseases / Q000175:diagnosis / Q000821:virology*; D006041:Goats; D006115:Greece; D007558:Italy; D008969:Molecular Sequence Data; D009923:Orf virus / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment; D012756:Sheep; D013045:Species Specificity
chemical_list: D004279:DNA, Viral