title: Chemical proteomics profiling of proteasome activity.
abstract: Proteolysis is a key mechanism for protein homeostasis in living cells. This process is effected by different classes of proteases. The proteasome is one of the most abundant and versatile proteases, bearing three different proteolytic active sites. The proteasome plays an important role in essential biological pathways such as antigen presentation, signal transduction, and cell-cycle control feedback loops. The aim of this work is to design novel chemical strategies for capturing, detection, identification, and quantification--in one word, profiling--the active protease fractions of interest, in cells of different phenotypes. Here, a set of chemistry-based functional proteomics techniques is demonstrated by profiling the multi-catalytic protease activities of the proteasome. Importantly, functional profiling is complementary to expression level profiling and is an indispensable parameter for better understanding of mechanisms underlying biological processes.
authors.affiliation: Leiden Institute of Chemistry, Leiden University, the Netherlands.
authors.forename: Martijn
authors.initials: M
authors.lastname: Verdoes
authors.forename: Celia R
authors.initials: CR
authors.lastname: Berkers
authors.forename: Bogdan I
authors.initials: BI
authors.lastname: Florea
authors.forename: Paul F
authors.initials: PF
authors.lastname: van Swieten
authors.forename: Herman S
authors.initials: HS
authors.lastname: Overkleeft
authors.forename: Huib
authors.initials: H
authors.lastname: Ovaa
mesh_terms: D015153:Blotting, Western; D002384:Catalysis; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D006367:HeLa Cells; D006801:Humans; D008954:Models, Biological; D008956:Models, Chemical; D015345:Oligonucleotide Probes / Q000737:chemistry; D010447:Peptide Hydrolases / Q000737:chemistry; D010641:Phenotype; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
chemical_list: D015345:Oligonucleotide Probes; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex