[Effect of fluid shear stress time on the morphological change of rat polarized osteoclasts].
OBJECTIVE
To elucidate the morphological change of rat polarized osteoclasts influenced by different fluid shear stress time.


METHODS
Special TRAP dyeing and bone resorption of rat polarized osteoclasts were performed. A fluid shear stress device made in the authors' laboratory was used to enforce shear stress at different times on the polarized osteoclasts for observation on their morphological change and analysis on the kind of change by image analytical software. The time points were 0,15,30,45,60,75,90,105,120 min; the shear stress was 0.29 Pa.


RESULTS
Morphologically, the polarized osteoclasts were bigger than other monocytes. They were each about 30 microm in diameter, having about 20 round and black nuclei in cytoplasm. There were large numbers of cellular microfilaments. The areas and diameters of these osteoclasts increased with the times. Statistically, one-way ANOVA showed significant differences between the 0 min group and 30 min, 105 min groups respectively.


CONCLUSIONS
In this study, the morphological change of polarized osteoclasts fluctuated with the increase of fluid shear stress time, and their areas and diameters exhibited a tendency to increase.
Zhang
Oing-hong
OH
Department of Prosthetics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Liang
Xing
X
Dong
Qiang
Q
Chen
Ming
M
Xu
Ling
L
Xia
Lu
L
D000818:Animals; D002478:Cells, Cultured; D008297:Male; D008964:Molar, Third / Q000166:cytology; D010010:Osteoclasts / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033081:Shear Strength; D013997:Time Factors