title: Neostriatal cytoskeleton changes following perinatal asphyxia: effect of hypothermia treatment.
abstract: Long-term changes of different types of neurofilaments (NF) and glial fibrillar acid protein (GFAP) were studied in neostriatal rat subjected to perinatal asphyxia (PA) under normothermic and hypothermic (15 degrees C) conditions, using immunohistochemistry for light and electron microscopy. Neostriatal neurons of 6-month-old rats that were subjected to 19 and 20 min of PA, showed an increase of NF 200 kDa immunostaining mainly in the axon fascicles in comparison with the control and hypothermia groups. In contrast, no alterations were seen with NF68 and NF160 neurofilament antibodies. Furthermore, the same PA groups showed astroglial cells with enhanced GFAP immunoreactivity, evidencing a typical astroglial reaction with a clear hypertrophy of these cells. A quantitative image analysis confirmed these observations. Hypothermic treated animals did show neither astroglial nor neuronal cytoskeletal changes in comparison to the control group. These findings showed that PA produces chronic cytoskeletal alterations in the neostriatum cells that can be prevented by hypothermia.
authors.affiliation: Instituto de Biología Celular y Neurociencias Prof. E. De Robertis, Facultad de Medicina Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Elisa
authors.initials: E
authors.lastname: Cebral
authors.forename: Francisco
authors.initials: F
authors.lastname: Capani
authors.forename: Asia
authors.initials: A
authors.lastname: Selvín-Testa
authors.forename: Manuel Rey
authors.initials: MR
authors.lastname: Funes
authors.forename: Héctor
authors.initials: H
authors.lastname: Coirini
authors.forename: C Fabián
authors.initials: CF
authors.lastname: Loidl
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001237:Asphyxia / Q000378:metabolism* / Q000628:therapy; D003599:Cytoskeleton / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007035:Hypothermia; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D016253:Microscopy, Immunoelectron / Q000379:methods; D017072:Neostriatum / Q000378:metabolism* / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D005904:Glial Fibrillary Acidic Protein