title: 55 kDa outer-membrane protein from short-chain fatty acids exposed Salmonella enterica serovar Typhi induces apoptosis in macrophages.
abstract: Various pathogens including Salmonella species are known to induce apoptosis in host cell types during their infection processes. However, the bacterial components capable of inducing apoptosis have not been fully understood. It is now known that in vivo expression of virulence determinants differ from the expression under in vitro conditions. Therefore, in the present study, attempts were made to evaluate the apoptotic potential of outer-membrane protein (OMP) from short-chain fatty acids (SCFA) exposed Salmonella enterica serovar Typhi. Short-chain fatty acids exposure is one of the in vivo stresses encountered by the pathogen in the intestine. Therefore, to simulate the in vivo condition, S. enterica serovar Typhi was grown in the presence of SCFA and its OMP profile was analyzed. The apoptotic potential of 55 kDa protein expressed with enhanced intensity under the SCFA stress was evaluated. Murine peritoneal macrophages interacted with 55 kDa protein showed DNA fragmentation, changes in fluorescence and exposure of phosphatidylserine on their outer leaflets. Levels of nitrite and citrulline were found to be increased in the supernatant of macrophages after interacting them with 55 kDa protein. However, the enzymatic activity of superoxide dismutase was found to be decreased as compared to that of the control (uninteracted) macrophages. These observations indicate that increased levels of nitrite and decreased levels of superoxide dismutase may be one of the mechanisms to induce apoptosis in macrophages by SCFA induced 55 kDa OMP. These findings may help us better understand the pathophysiology of the disease during the host pathogen interactions.
authors.affiliation: Department of Microbiology, Panjab University, Chandigarh, India.
authors.forename: Harish
authors.initials: H
authors.lastname: Chander
authors.forename: Siddarth
authors.initials: S
authors.lastname: Majumdar
authors.forename: Sunita
authors.initials: S
authors.lastname: Sapru
authors.forename: Praveen
authors.initials: P
authors.lastname: Rishi
mesh_terms: D000818:Animals; D017209:Apoptosis; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology*; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D005434:Flow Cytometry; D017737:Macrophages, Peritoneal / Q000166:cytology* / Q000382:microbiology / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D012485:Salmonella typhi / Q000472:pathogenicity*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D005232:Fatty Acids, Volatile