[Expression and effects of nuclear distribution C (NUDC) protein in nasopharyngeal carcinoma cell lines].
OBJECTIVE
Nuclear migration is essential for growth, development, and cellular function of eukaryotes. Nuclear distribution C (NUDC) protein plays an important role in nuclear migration. This study was to detect the expression of NUDC protein in nasopharyngeal carcinoma (NPC) cell lines CNE-2 and HNE-2, and to investigate the effect of anti-NUDC antibody on the growth of both cell lines.


METHODS
Soluble fusion protein GST-NUDC was expressed in the cytoplasm of Escherichia coli, and its poly-antibody was prepared. The subcellular localization of NUDC protein in CNE-2 and HNE-2 cells was detected with indirect immunofluorescent staining, the expression of NUDC protein in CNE-2 and HNE-2 cells was detected by Western blot, the effect of anti-NUDC antibody on the growth of CNE-2 and HNE-2 cells was detected by MTT assay.


RESULTS
NUDC protein was expressed in both nasopharyngeal non-cancerous tissues and CNE-2 and HNE-2 cells, and its expression was higher in CNE-2 and HNE-2 cells than in nasopharyngeal non-cancerous tissues. NUDC protein in CNE-2 and HNE-2 cells was distributed mainly in the karyolemma of cytoplasm, and in nuclei asymmetrically. Anti-NUDC antibody inhibited the growth of CNE-2 and HNE-2 cells in a dose-dependent manner.


CONCLUSIONS
NUDC protein is expressed highly in CNE-2 and HNE-2 cells. Anti-NUDC antibody could inhibit the growth of CNE-2 and HNE-2 cells.
Chen
Yan-Qiu
YQ
Hospital of Otorhinolaryngology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510080, P. R. China. frankcyq2002@yahoo.com.cn
Li
Tian-Ying
TY
Qu
Shen-Hong
SH
Tang
Xiao-Jiang
XJ
D000906:Antibodies / Q000494:pharmacology; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D018797:Cell Cycle Proteins; C512648:NUDC protein, human; D009687:Nuclear Proteins