[Molecular genetic analysis of the biological properties of highly pathogenic influenza A/H5N1 virus strains isolated from wild birds and poultry during epizooty in Western Siberia (July 2005)].
The full-size genomes of 2 highly pathogenetic avian influenza (HPAI) virus strains isolated from a wild great-crested grebe (A/Grebe/Novosibirsk/29/05) and a domestic duck (A/Duck/Novosibirsk/56/05) in the tract of the Chany hollow, Barabino forest-steppe (Novosibirsk Region) during the epizootic outbreak in the summer of 2005. The reproductive properties of these strains successively increase in the series of cell lines BHK-2 --> LEH --> Vero-E6 --> MDCK --> PS. A/Grebe/Novosibirsk/29/05 and A/Duck/Novosibirsk/56/05 were shown to be genetically close in all genomic segments to both each other and a group of HPAI/H5N1 A/Qinghai 05 strains isolated from wild birds on the Kukunor Lake in the northwestern province of Tsinkhai, China, in May 2005. All the above strains have the HPAI/H5-specific amino acid sequence of a proteolytic cleavage site (PQGERRRKKRGLF) with Lys-627 in the protein PB2 (which is associated with increased virulence to mammalian cells), Glu-92 in the protein NS1 (that suppresses an antiviral response in the host), Ser-31 in M2 (that is a marker of rimantadine/amantadine sensitivity), 20-member amino acid deletion in the protein NA (positions 49-68) that is a marker of affiliation to the so-called genotype Z and of increased tropism to poultry.
L'vov
D K
DK
Prilipov
A G
AG
Shchelkanov
M Iu
MIu
Deriabin
P G
PG
Shilov
A A
AA
Grebennikova
T V
TV
Sadykova
G K
GK
Liapina
O V
OV
D000547:Amantadine / Q000494:pharmacology; D019943:Amino Acid Substitution; D000818:Animals; D000829:Animals, Domestic / Q000821:virology; D000835:Animals, Wild / Q000821:virology; D000998:Antiviral Agents / Q000494:pharmacology; D001717:Birds / Q000821:virology; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004196:Disease Outbreaks; D004285:Dogs; D016679:Genome, Viral; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000187:drug effects / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D005585:Influenza in Birds / Q000453:epidemiology / Q000821:virology*; D007668:Kidney; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012299:Rimantadine / Q000494:pharmacology; D012800:Siberia / Q000453:epidemiology; D013045:Species Specificity; D013552:Swine; D014763:Viral Matrix Proteins / Q000235:genetics / Q000502:physiology; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence / Q000235:genetics; D000086082:Viroporin Proteins
D000547:Amantadine; D000998:Antiviral Agents; D010447:Peptide Hydrolases; D012324:RNA-Dependent RNA Polymerase; D012299:Rimantadine; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins; C061290:PA protein, influenza viruses