CD4+CD25+ regulatory T cells and CD1-restricted NKT cells do not mediate facial motoneuron survival after axotomy.
CD4+ T cells rescue facial motoneurons (FMN) from axotomy-induced cell death. The objective of this study is to determine if the CD4+ T regulatory subsets, CD4+CD25+ T or CD1d-restricted NKT cells are critical for FMN survival after facial nerve axotomy. Surviving FMN within facial motor nuclei from axotomized and control sides 4 weeks after axotomy were counted to determine percent FMN survival. Data generated by applying this paradigm to recombination activating gene-2-deficient mice reconstituted with CD4+ T cells depleted of CD4+CD25+ T cells and to CD1-/- mice, deficient in CD1d-restricted NKT cells, suggest that neither regulatory CD4+ T subset is critical for FMN survival.
DeBoy
Cynthia A
CA
Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Chicago, Maywood, IL 60153, and Research and Development Service, Hines VA Hospital 60141, USA. cdeboy3@jhmi.edu
Byram
Susanna C
SC
Serpe
Craig J
CJ
Wisuri
Danielle
D
Sanders
Virginia M
VM
Jones
Kathryn J
KJ
D000818:Animals; D018949:Antigens, CD1 / Q000502:physiology*; D019771:Axotomy; D002470:Cell Survival; D004268:DNA-Binding Proteins / Q000502:physiology; D005154:Facial Nerve / Q000502:physiology*; D005260:Female; D007694:Killer Cells, Natural / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009046:Motor Neurons / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*
D018949:Antigens, CD1; D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse