Free diC14-amidine liposomes inhibit the TNF-alpha secretion induced by CpG sequences and lipopolysaccharides: role of lipoproteins.
It has been shown that a preinjection of diC14-amidine cationic liposomes decreased TNF-alpha secretion induced by lipoplexes intravenous injection. We showed here that free cationic liposomes inhibit CpG sequences- or lipopolysaccharides-induced TNF-alpha secretion by macrophages. Surprisingly, this effect was strictly dependent on serum. Free cationic liposomes alone did not reveal any anti-inflammatory activity. Low-density lipoproteins and triglyceride-rich lipoproteins were identified as the serum components that confer to the liposomes an anti-inflammatory activity. Lipid fractions of these lipoproteins were able to reproduce the effect of the total lipoproteins and could inhibit, in association with diC14-amidine liposomes, the CpG-induced TNF-alpha secretion. Serum components confer to cationic liposomes new properties that can be used to modulate the inflammatory response directed against CpG sequences and lipopolysaccharides.
Lonez
Caroline
C
Laboratory of Structure and Function of Biological Membranes, Center of Structural Biology and Bioinformatics, Université Libre de Bruxelles, Belgium.
Vandenbranden
Michel
M
Ouali
Mustapha
M
Legat
Amandine
A
Ruysschaert
Jean-Marie
JM
Elouahabi
Abdelatif
A
D000578:Amidines / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism; D002478:Cells, Cultured; D018899:CpG Islands; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism*; D008074:Lipoproteins / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008081:Liposomes / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009841:Oligonucleotides / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000578:Amidines; D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D008081:Liposomes; C089665:N-t-butyl-n'-tetradecyl-3-tetradecylaminopropionamidine; D009841:Oligonucleotides; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha