Effects of histone deacetylase inhibitors on transcriptional regulation of the hsp70 gene in Drosophila.
Histone acetyltransferases/deacetylases contribute to the activation or inactivation of transcription by modifying the structure of chromatin. Here we examined the effects of histone deacetylase inhibitors (HDIs), trichostatin A, and sodium butyrate on hsp70 gene transcriptional regulation in Drosophila. The chromatin immunoprecipitation assays revealed that HDI treatments induced the hyperacetylation of histone H3 at the promoter and the transcribing regions of hsp70 gene, increased the accessibility of heat-shock factor to target heat-shock element, and promoted the RNA polymerase II-mediated transcription. Moreover, the quantitative real-time PCR confirmed that the HDI-induced hyperacetylation of histone H3 enhanced both the basal and the inducible expression of hsp70 mRNA level. In addition, the acetylation level of histone H3 at the promoter exhibited a fluctuated change upon the time of heat shock. These experimental data implicated a causal link between histone acetylation and enhanced transcription initiation of hsp70 gene in Drosophila.
Zhao
Yan Mei
YM
The Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
Chen
Xia
X
Sun
Hui
H
Yuan
Zhi Gen
ZG
Ren
Guo Ling
GL
Li
Xiao Xue
XX
Lu
Jun
J
Huang
Bai Qu
BQ
D000107:Acetylation; D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology; D007814:Larva; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D015533:Transcriptional Activation
D002087:Butyrates; D018840:HSP70 Heat-Shock Proteins; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A; D012319:RNA Polymerase II; D006655:Histone Deacetylases