title: The bcl-2 major breakpoint region (mbr) possesses transcriptional regulatory function.
abstract: The bcl-2 major breakpoint region (mbr), located within the 3'-UTR of the bcl-2 gene, is the site of the most common chromosomal translocation, t(14;18) (q32;q21), which occurs in follicular lymphoma. The mbr forms a triplex DNA structure under physiological conditions and the transcription factor special AT-rich sequence-binding protein 1 (SATB1) binds immediately downstream of the mbr. These observations raise the possibility that the mbr may be involved in regulation of bcl-2 gene expression. We investigated the role of the bcl-2 mbr on reporter gene activity and the relevance of SATB1 to this function in a variety of cell lines. We found that the mbr up-regulated reporter gene expression. Deletion of the 37-bp AT-rich SATB1 binding site abolished the bcl-2 mbr regulation of reporter gene expression. Overexpression of SATB1 enhanced bcl-2 mbr up-regulation of the reporter gene activity. Our data strongly demonstrated that the bcl-2 mbr possessed regulatory function that was related to SATB1.
authors.affiliation: Key Laboratory of Human Functional Genomics of Jiangsu Province, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
authors.forename: Changyan
authors.initials: C
authors.lastname: Ma
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.forename: Linda K
authors.initials: LK
authors.lastname: Durrin
authors.forename: Yujie
authors.initials: Y
authors.lastname: Sun
mesh_terms: D020413:3' Untranslated Regions / Q000737:chemistry; D001665:Binding Sites; D045744:Cell Line, Tumor; D005260:Female; D017930:Genes, Reporter; D006801:Humans; D019169:Jurkat Cells; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008214:Lymphocytes; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D036961:Matrix Attachment Region Binding Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; C076189:SATB1 protein, human; D008156:Luciferases