title: Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis.
abstract: The links between the cell cycle machinery and the cytoskeletal proteins controlling cytokinesis are poorly understood. The small guanine nucleotide triphosphate (GTP)-binding protein RhoA stimulates type II myosin contractility and formin-dependent assembly of the cytokinetic actin contractile ring. We found that budding yeast Polo-like kinase Cdc5 controls the targeting and activation of Rho1 (RhoA) at the division site via Rho1 guanine nucleotide exchange factors. This role of Cdc5 (Polo-like kinase) in regulating Rho1 is likely to be relevant to cytokinesis and asymmetric cell division in other organisms.
authors.affiliation: Department of Pediatric Oncology, Dana-Farber Cancer Institute and Division of Hematology/Oncology, Children's Hospital Boston and Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Keiko
authors.initials: K
authors.lastname: Kono
authors.forename: Drew M
authors.initials: DM
authors.lastname: Lowery
authors.forename: Sara
authors.initials: S
authors.lastname: Bartolini
authors.forename: Michael B
authors.initials: MB
authors.lastname: Yaffe
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ohya
authors.forename: David
authors.initials: D
authors.lastname: Pellman
mesh_terms: D000199:Actins / Q000378:metabolism; D020816:Amino Acid Motifs; D000705:Anaphase; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D048749:Cytokinesis; D020662:Guanine Nucleotide Exchange Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D008938:Mitosis; D009154:Mutation; D010766:Phosphorylation; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D000199:Actins; C105191:Bni1 protein, S cerevisiae; D018797:Cell Cycle Proteins; D020662:Guanine Nucleotide Exchange Factors; D008840:Microfilament Proteins; C490929:ROM2 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C448998:TUS1 protein, S cerevisiae; D006160:Guanosine Triphosphate; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C081927:CDC5 protein, S cerevisiae; C051302:RHO1 protein, S cerevisiae; D020741:rho GTP-Binding Proteins