title: Simple and rapid determination of hydrogen peroxide using phosphine-based fluorescent reagents with sodium tungstate dihydrate.
abstract: A simple batch method for the fluorometric determination of hydrogen peroxide using phosphine-based fluorescent reagents has been developed. A rapid, mild and selective derivatization reaction was achieved by adding sodium tungstate dihydrate to the reaction mixture of hydrogen peroxide and a phosphine-based fluorescent reagent. When 4-diphenylphosphino-7-methylthio-2,1,3-benzoxadiazole was used as a reagent, the derivatization reaction was completed after 2 min at room temperature. The calibration curve was linear between 12.5 and 500 ng hydrogen peroxide in a 10 microL sample solution. This method is accurate and has potential for on-line applications.
authors.affiliation: Department of Analytical Chemistry, School of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan. ff57007@mail.ecc.u-tokyo.ac.jp
authors.forename: Maki
authors.initials: M
authors.lastname: Onoda
authors.forename: Takefumi
authors.initials: T
authors.lastname: Uchiyama
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Mawatari
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Kaneko
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nakagomi
mesh_terms: D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010720:Phosphines / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D017973:Tungsten Compounds / Q000032:analysis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D010720:Phosphines; D017973:Tungsten Compounds; C025399:sodium tungstate(VI); D006861:Hydrogen Peroxide