title: A sensitive method to monitor ectodomain shedding of ligands of the epidermal growth factor receptor.
abstract: All ligands of the epidermal growth factor receptor (EGFR) are made as membrane anchored precursors that can be proteolytically processed and released from the plasma membrane. This process, which is referred to as protein ectodomain shedding, is emerging as a key regulator of the function of EGFR ligands. In light of the important roles of EGFR signaling in development and disease, it will be important to understand more about the regulation of proteolytic processing of EGFR ligands. This chapter describes a sensitive and semiquantitative method to measure ectodomain shedding of EGFR ligands that was designed to facilitate studies of this process in cells.
authors.affiliation: Arthritis and Tissue Degeneration Program, Hospital for Special Surgery, Weill Medical College of Cornell University, New York, NY, USA.
authors.forename: Umut
authors.initials: U
authors.lastname: Sahin
authors.forename: Gisela
authors.initials: G
authors.lastname: Weskamp
authors.forename: Yufang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Valerie
authors.initials: V
authors.lastname: Chesneau
authors.forename: Keisuke
authors.initials: K
authors.lastname: Horiuchi
authors.forename: Carl P
authors.initials: CP
authors.lastname: Blobel
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D066246:ErbB Receptors / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D008024:Ligands; D051379:Mice; D012680:Sensitivity and Specificity
chemical_list: D008024:Ligands; D066246:ErbB Receptors; D000469:Alkaline Phosphatase