An improved method for Southern DNA and Northern RNA blotting using a Mupid-2 Mini-Gel electrophoresis unit.
An improved method for Southern DNA and Northern RNA blotting using the Mupid-2 Mini-Gel System is described. We get sharp and clear bands in Southern and Northern blotting after only 30 min short gel electrophoresis instead of the several hours large gel electrophoresis of conventional methods. The high electrical voltage with a pulse-like current of the Mupid-2 Mini-Gel System also allows reduction of the amount of formaldehyde, a harmful reagent, from the gel running buffer in RNA blotting. This minor modification of DNA and RNA blotting technique enables us to perform the complete experimental procedure more quickly economically in less space, than conventional Southern and Northern blotting, as well as using an extremely small amount of formaldehyde in RNA blotting.
Furuya
Hirokazu
H
Clinical Research Center, Japan. furuya@neuro.med.kyushu-u.ac.jp
Yamada
Takeshi
T
Ikezoe
Koji
K
Ohyagi
Yasumasa
Y
Fukumaki
Yasuyuki
Y
Fujii
Naoki
N
D015152:Blotting, Northern / Q000379:methods; D015139:Blotting, Southern / Q000379:methods; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D012313:RNA / Q000032:analysis*
D012313:RNA; D004247:DNA