[Effect of advanced oxidation protein products on nitric oxide production in mouse peritoneal macrophages].
OBJECTIVE
To investigate the effect of advanced oxidation protein products (AOPP) on nitric oxide (NO) production in mouse peritoneal macrophages (MPMs).


METHODS
MPMs were incubated in the absence or presence of lipopolysaccharide (LPS) with AOPP-modified bovine serum albumin (BSA) prepared by exposure of BSA to hypoclorous acid or pre-treated with AOPP-BSA and subsequent stimulation with LPS. NO production in the supernatants of the culture media was determined spectrophotometrically using Griess method. The cell viability was measured by MTT assay.


RESULTS
BSA induced significant NO production in MPMs. AOPP modification of BSA significantly inhibited NO production, and AOPP-BSA exhibited time- and dose-dependent inhibition of NO production induced by LPS in MPMs incubated together with LPS or pre-treated before LPS stimulation.


CONCLUSIONS
AOPP-BSA is capable of inhibiting inducible NO production in MPMs.
Li
Zhong-hai
ZH
Department of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. loy5460@yahoo.com.
Liu
Shang-xi
SX
Hou
Fan-fan
FF
Wang
Yan-qun
YQ
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D017127:Glycation End Products, Advanced / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors
D003470:Culture Media; D017127:Glycation End Products, Advanced; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D012710:Serum Albumin, Bovine; D009569:Nitric Oxide