Inhibitory effect of fermented milk on delayed-onset muscle damage after exercise.
Milk fermented with a starter containing Lactobacillus helveticus and Saccharomyces cerevisiae is drunk on a daily basis by many people in Japan and has several beneficial effects. We studied the influence of this fermented milk product on muscle damage after prolonged exercise in rats. Wistar rats were divided into four groups: rested controls, rested rats given fermented milk diet, exercised rats and exercised rats given fermented milk diet. After 3 weeks of acclimatization, both exercise groups were made to run on a treadmill at 26 m/min for 60 min. Exercise increased the serum creatine kinase level, as well as myeloperoxidase activity and the level of thiobarbituric-acid-reactive substances in the gastrocnemius muscle after 24 h. These changes were ameliorated by intake of fermented milk. An increase of CINC-1 was also ameliorated by fermented milk. Furthermore, milk diet increased the mRNA and protein levels of protective proteins such as antioxidants and chaperone proteins. These results indicate that fermented milk can ameliorate delayed-onset muscle damage after prolonged exercise, which is associated with an increased antioxidant capacity of muscles.
Aoi
Wataru
W
Department of Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. waoi@koto.kpu-m.ac.jp
Naito
Yuji
Y
Nakamura
Teppei
T
Akagiri
Satomi
S
Masuyama
Akihiro
A
Takano
Toshiaki
T
Mizushima
Katsura
K
Yoshikawa
Toshikazu
T
D000818:Animals; D000975:Antioxidants / Q000032:analysis; D015153:Blotting, Western; D054360:Chemokine CXCL1; D019743:Chemokines, CXC / Q000032:analysis; D003402:Creatine Kinase / Q000097:blood; D043302:Cultured Milk Products / Q000382:microbiology / Q000502:physiology*; D004032:Diet; D052198:Lactobacillus helveticus; D008297:Male; D009124:Muscle Proteins / Q000032:analysis / Q000235:genetics; D018482:Muscle, Skeletal / Q000201:enzymology / Q000293:injuries*; D009504:Neutrophils / Q000201:enzymology / Q000502:physiology; D009195:Peroxidase / Q000097:blood; D005082:Physical Exertion / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae; D013482:Superoxide Dismutase / Q000032:analysis / Q000235:genetics; D000072105:Superoxide Dismutase-1; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D000099156:Superoxide Dismutase 2
D000975:Antioxidants; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D003402:Creatine Kinase; D009124:Muscle Proteins; D009195:Peroxidase; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D017392:Thiobarbituric Acid Reactive Substances; D000099156:Superoxide Dismutase 2; C513297:Cxcl1 protein, rat; C000606292:Sod1 protein, rat