title: Cantilever arrays for multiplexed mechanical analysis of biomolecular reactions.
abstract: Microchips containing arrays of cantilever beams have been used to mechanically detect and quantitatively analyze multiple reactions of DNA hybridization and antigen-antibody binding simultaneously. The reaction-induced deflection of a cantilever beam reflects the interplay between strain energy increase of the beam and the free energy reduction of a reaction, providing an ideal tool for investigating the connection between mechanics and chemistry of biomolecular reactions. Since free energy reduction is common for all reactions, the cantilever array forms a universal platform for label-free detection of various specific biomolecular reactions. A few such reactions and their implications in biology and biotechnology are discussed.
authors.affiliation: Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Yue
authors.forename: Jeanne C
authors.initials: JC
authors.lastname: Stachowiak
authors.forename: Arunava
authors.initials: A
authors.lastname: Majumdar
mesh_terms: D000937:Antigen-Antibody Reactions; D001696:Biomechanical Phenomena; D002138:Calibration; D004277:DNA, Single-Stranded / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D011485:Protein Binding
chemical_list: D004277:DNA, Single-Stranded