Reactive oxygen species and antioxidants relationship in the internal spermatic vein blood of infertile men with varicocele.
OBJECTIVE
To assess the relation of reactive oxygen species (ROS) and antioxidants in the internal spermatic vein blood compared to the peripheral venous blood.


METHODS
Sixty-eight infertile oligoasthenozoospemic patients associated with varicocele were investigated. During inguinal varicocelectomy, blood samples of internal spermatic as well as median cubital veins were withdrawn. Three ROS factors (malondialdehyde [MDA], hydrogen peroxide H(2)O(2), nitric oxide [NO]) and four antioxidants (superoxide dismutase [SOD], catalase [Cat], glutathione peroxidase [GPx] and vitamin C) were estimated in these blood samples.


RESULTS
Mean levels of tested ROS factors were significantly higher in the internal spermatic venous blood compared to those in the peripheral one (mean+/-SD) (MDA 18.7+/-1.4 nmol/mL vs. 15.4+/-1.4 nmol/mL, H(2)O(2) 43.6+/-8.0 micromol/mL vs. 30.8+/-8.1 micromol/mL, NO 2.3+/-0.5 nmol/L vs. 1.6+/-0.4 nmol/L, P<0.01). Mean levels of tested antioxidants were significantly lower in the internal spermatic venous blood compared to those in the peripheral one (superoxide dismutase 1 690.7+/-130.0 U/mL vs. 1 818.5+/-143.0 U/mL, catalase 38.9+/-6.1 mol/L vs. 47.9+/-10.2 mol/L, GPx 20.4+/-8.1 U/mL vs. 23.0+/-8.4 U/mL, vitamin C 0.3+/-0.1 vs. 0.4+/-0.1 mg/dL, P<0.05).


CONCLUSIONS
Internal spermatic venous blood of infertile male cases associated with varicocele demonstrated elevated levels of ROS and decreased levels of antioxidants compared to peripheral venous circulation.
Mostafa
Taymour
T
Andrology Deptatment, Faculty of Medicine, Cairo University, Cairo 12311, Egypt. taymour1155@link.net
Anis
Tarek H
TH
Ghazi
Sherif
S
El-Nashar
Abdel Rahman
AR
Imam
Hager
H
Osman
Ihab A
IA
D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000097:blood; D002374:Catalase / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007248:Infertility, Male / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D011446:Prospective Studies; D017382:Reactive Oxygen Species; D013085:Spermatic Cord / Q000098:blood supply*; D013482:Superoxide Dismutase / Q000097:blood; D014646:Varicocele / Q000201:enzymology / Q000378:metabolism*; D014680:Veins / Q000201:enzymology / Q000378:metabolism*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid