Cellular and biochemical parameters of exercise-induced oxidative stress: relationship with training levels.
To better clarify the relationship between physical activity and oxidative stress, we determined the effects of a maximal test in 18 young subjects with different training levels (six professional Athletes and 12 non-agonists (NA)). Redox homeostasis (total antioxidant activity (TAS), vitamin C and glutathione (GSH)), oxidative damage (diene conjugation and hemolysis), lymphocyte cell death and repair systems (apoptosis, micronuclei and Hsp70 expression) were evaluated. We found that agonistic training led to a chronic oxidative insult (high baseline values of oxidized glutathione (GSSG), micronuclei and hemolysis). On the contrary, NA with the lowest level of training frequency showed a well balanced profile at rest, but they were more susceptible to exercise-induced variations (GSSG/GSH and diene increased values), respect to the NA with an higher level of training. As almost all the parameters employed in this study showed inter-individual variations, the GSSG/GSH ratio remains the most sensitive and reliable marker of oxidative stress, accordingly with other data just reported in the literature.
Pittaluga
Monica
M
Laboratory of Biology and Biochemistry of Movement, Department of Sciences of Human Movement and Sport, IUSM-University of Sport and Movement Sciences, Foro Italico piazza Lauro de Bosis 6, 00194, Rome, Italy. pittaluga@iusm.it
Parisi
Paolo
P
Sabatini
Stefania
S
Ceci
Roberta
R
Caporossi
Daniela
D
Valeria Catani
M
M
Savini
Isabella
I
Avigliano
Luciana
L
D000328:Adult; D017209:Apoptosis; D015415:Biomarkers; D004260:DNA Repair; D015444:Exercise / Q000502:physiology*; D005978:Glutathione / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010809:Physical Fitness / Q000502:physiology*
D015415:Biomarkers; D005978:Glutathione