title: Contribution of the intra- and intermolecular routes in autocatalytic zymogen activation: application to pepsinogen activation.
abstract: Taking as the starting point a recently suggested reaction scheme for zymogen activation involving intra- and intermolecular routes and the enzyme-zymogen complex, we carry out a complete analysis of the relative contribution of both routes in the process. This analysis suggests the definition of new dimensionless parameters allowing the elaboration, from the values of the rate constants and initial conditions, of the time course of the contribution of the two routes. The procedure mentioned above related to a concrete reaction scheme is extrapolated to any other model of autocatalytic zymogen activation involving intra- and intermolecular routes. Finally, we discuss the contribution of both of the activating routes in pepsinogen activation into pepsin using the values of the kinetic parameters given in the literature.
authors.affiliation: Grupo de Modelización en Bioquímica, Departamento de Química-Física, Universidad de Castilla-La Mancha, Albacete, Spain. ramon.varon@uclm.es
authors.forename: Ramón
authors.initials: R
authors.lastname: Varón
authors.forename: Matilde E
authors.initials: ME
authors.lastname: Fuentes
authors.forename: Manuela
authors.initials: M
authors.lastname: García-Moreno
authors.forename: Francisco
authors.initials: F
authors.lastname: Garcìa-Sevilla
authors.forename: Enrique
authors.initials: E
authors.lastname: Arias
authors.forename: Edelmira
authors.initials: E
authors.lastname: Valero
authors.forename: Enrique
authors.initials: E
authors.lastname: Arribas
mesh_terms: D000465:Algorithms; D002384:Catalysis; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical; D020116:Pepsinogen A / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004792:Enzyme Precursors; D020116:Pepsinogen A