IgM antibody response to antigens prepared from vegetative and coccoid forms of Helicobacter pylori.
This report describes the utility of antigens prepared from different coccoid and spiral forms of Helicobacter pylori in a serological method. The presence of IgM antibody to H. pylori was determined in 22 human sera on antigens prepared from 24 strains of H. pylori. Antigens prepared from spiral form of certain strains of H. pylori detected IgM in all confirmed positive sera. Antigens obtained from the coccoid cells of the same strains could not completely detect IgM in the same sera. Testing sera on boosted antigens of the coccoid cells showed reduction in the number of false negative, indicating that the coccoid cells do not have one or more antigenic fractions essential for accurate detection of antibody. Our data suggest that H. pylori may lose CagA during the coccoid conversion process and regain it in the spiral form. In conclusion, we suggest that the antigen used for the detection of antibodies to H. pylori in serological methods should contain a broad spectrum of antigenic fractions and should be prepared from certain strains and culturable cells of H. pylori.
Shahamat
Manoucher
M
University of Maryland, Biotechnology Institute, Baltimore, MD 21202, USA.
Alem
Navid
N
Asalkhou
Mandana
M
Hamedi
Nina
N
Alem
Neda
N
Morshedizadeh
Kasra
K
Alem
Mehdi
M
D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000145:classification / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D016036:Seroepidemiologic Studies
D000942:Antigens, Bacterial; D007075:Immunoglobulin M