Evaluation of the usefulness of anti-glyceraldehyde-3-phosphate dehydrogenase antibodies as a treatment for invasive candidiasis in a murine model.
We have evaluated the effect of antibodies against the Candida albicans glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a potential immunotherapeutic treatment for acute invasive candidiasis in a murine model of infection. Three different approaches were assayed: (i) active immunization of mice using recombinant His-tagged GAPDH, (ii) treatment of fungal yeast cells with anti-GAPDH antibodies prior to infection, and (iii) passive transfer of polyclonal anti-GAPDH antibodies. Results showed that all three approaches, although tending to show a slight beneficial effect in some instances, fail to have a relevant and statistically significant effect on the infection course, determined by survival curves and fungal burden in kidneys. This suggests that the cell wall-associated GAPDH of C. albicans, despite its potential role in virulence, does not appear to be a suitable target protein for the development of immunotherapeutic strategies against candidiasis, although further studies may be required to confirm this observation.
Gil
María Luisa
ML
Departament de Microbiologia i Ecologia, Universitat de València, Avda. Vicent Andrés Estellés s/n, 46100, Burjassot, Spain.
Dagan
Shlomo
S
Eren
Rachel
R
Gozalbo
Daniel
D
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology / Q000627:therapeutic use*; D000946:Antigens, Fungal / Q000276:immunology; D002176:Candida albicans / Q000201:enzymology / Q000276:immunology*; D002177:Candidiasis / Q000382:microbiology / Q000628:therapy*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007114:Immunization; D007116:Immunization, Passive; D051379:Mice; D008813:Mice, Inbred ICR; D011817:Rabbits
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases