[Real-time fluorescence quantitative PCR in detecting ribosome protein S13 (RPS13) gene expression in NK/T cell lymphoma].
OBJECTIVE
To quantitatively detect the expression level of ribosome protein S13 (RPS13) in NK/T cell lymphoma.


METHODS
The total RNA isolated from the parafin-embedded tissue of NK/T cell lymphoma was reversely transcribed into cDNA. The real-time fluorescence quantitative PCR technology method was used to analyze the expression level of RPS13 gene.


RESULTS
The real-time fluorescence quantitative PCR technology was successfully performed to precisely detect the mRNA level. The expression level of RPS13 gene in tumor tissue of 20 cases of NK/T cell lymphoma was dramatically lower than that in normal peripheral blood lymphocyte of healthy controls.


CONCLUSIONS
The RPS13 gene has lower expression level in tumor tissue cells of NK/T cell lymphoma than in normal lymphocyte, indicating that it plays an role in the development of the NK/T cell lymphoma.
Yang
Fan
F
Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Liu
Wei-ping
WP
He
Miao-xia
MX
Tang
Qiong-lan
QL
Zhao
Sha
S
Zhang
Wen-yan
WY
Xia
Qing-jie
QJ
Li
Gan-di
GD
D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000378:metabolism*; D009669:Nose Neoplasms / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012269:Ribosomal Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics
C047013:RPS13 protein, human; D012269:Ribosomal Proteins