title: Cartridge-based high-throughput purification of oligonucleotides for reliable oligonucleotide arrays.
abstract: A novel, cartridge-based procedure for the efficient and irreversible detritylation of oligonucleotides is reported. This method, combined with a process for the elimination of depurinated fragments produces, in a highly parallel fashion, oligonucleotides with better purity than those traditionally obtained using reversed-phase high-performance liquid chromotography purification. Our combined detritylation and purification methodology compares favorably with commercial cartridge-based purification systems. The benefits of working with pure oligonucleotides, with regard to higher signal and better signal linearity, are shown in array-based hybridization experiments.
authors.affiliation: Department of Genetics and Pathology, Uppsala University, 75185 Uppsala, Sweden.
authors.forename: Andrey
authors.initials: A
authors.lastname: Semenyuk
authors.forename: Matilda
authors.initials: M
authors.lastname: Ahnfelt
authors.forename: Camilla
authors.initials: C
authors.lastname: Estmer Nilsson
authors.forename: Xiao
authors.initials: X
authors.lastname: Yong Hao
authors.forename: Andras
authors.initials: A
authors.lastname: Földesi
authors.forename: Yu-Shu
authors.initials: YS
authors.lastname: Kao
authors.forename: Hong-Huei
authors.initials: HH
authors.lastname: Chen
authors.forename: Wei-Chen
authors.initials: WC
authors.lastname: Kao
authors.forename: Konan
authors.initials: K
authors.lastname: Peck
authors.forename: Marek
authors.initials: M
authors.lastname: Kwiatkowski
mesh_terms: D057927:Hydrophobic and Hydrophilic Interactions; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000706:statistics & numerical data; D015345:Oligonucleotide Probes; D009841:Oligonucleotides / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D014320:Triphenylmethyl Compounds / Q000737:chemistry
chemical_list: D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D014320:Triphenylmethyl Compounds