Comparison of a stool antigen detection kit and PCR for diagnosis of Entamoeba histolytica and Entamoeba dispar infections in asymptomatic cyst passers in Iran.
The present study was conducted to compare stool antigen detection with PCR for the diagnosis of Entamoeba sp. infection in asymptomatic cyst passers from Iran. Entamoeba dispar and, in one case, E. moshkovskii were the Entamoeba spp. found in the amebic cyst passers. There was a 100% correlation between the results from the TechLab E. histolytica II stool antigen kit and those from nested PCR. We concluded that E. dispar is much more common in asymptomatic cyst passers in Iran and that antigen detection and PCR are comparable diagnostic modalities.
Solaymani-Mohammadi
Shahram
S
Division of Intestinal and Genetal Protozoal Diseases, Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran Universoty of Medical Sciences, 14155 Tehran 6446, Iran.
Rezaian
Mostafa
M
Babaei
Zahra
Z
Rajabpour
Azam
A
Meamar
Ahmad R
AR
Pourbabai
Ahmad A
AA
Petri
William A
WA
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D016054:DNA, Protozoan / Q000032:analysis; D004747:Entamoeba / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004748:Entamoeba histolytica / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D005243:Feces / Q000469:parasitology*; D007492:Iran; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D011933:Reagent Kits, Diagnostic