title: [Detection of 16S ribosomal RNA gene of bacteria in prostate tissues of adults].
abstract: OBJECTIVE
  To investigate the role of bacteria in chronic prostatitis.
  
  
  METHODS
  Complete specimens of prostate were obtained from 140 organ donors, aged 20 - 35, at autopsy. A piece of tissue was collected from the peripheral zone of prostate from each specimen and was divided into 2 parts to undergo pathological examination and PCR so as to detect the 16S ribosomal RNA (16S rRNA) gene of bacteria.
  
  
  RESULTS
  Focal mild inflammation was shown in 46 of the 104 specimens (32.9%), including interstitial inflammation in 42 specimens, inflammation in both interstitial and body of gland in 3 specimens, and perigladulitis in 1 specimen. Twenty-seven of the 140 specimens (19.3%) were positive in 16S rRNA gene. The positive rate of 16S rRNA gene of the specimens with prostatitis was 48.9%, significantly higher than that of the specimens without prostatitis (5.3%, P < 0.001).
  
  
  CONCLUSIONS
  Bacteria may play an important role in the pathogenesis of chronic prostatitis.
authors.affiliation: Department of Urology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Hui-cong
authors.initials: HC
authors.lastname: Huang
authors.forename: Yi-rong
authors.initials: YR
authors.lastname: Yang
authors.forename: Jian-bo
authors.initials: JB
authors.lastname: Wu
authors.forename: Qiu-xiang
authors.initials: QX
authors.lastname: He
authors.forename: Qi-jian
authors.initials: QJ
authors.lastname: Zhu
authors.forename: Jian-ou
authors.initials: JO
authors.lastname: Chen
authors.forename: Si-qi
authors.initials: SQ
authors.lastname: Wang
authors.forename: Cheng-di
authors.initials: CD
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Xia
authors.forename: Long-jie
authors.initials: LJ
authors.lastname: Shen
mesh_terms: D000328:Adult; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000382:microbiology*; D011472:Prostatitis / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S