title: GMF-knockout mice are unable to induce brain-derived neurotrophic factor after exercise.
abstract: We earlier reported that overexpression of glia maturation factor (GMF) in cultured astrocytes enhances the production of brain-derived neurotrophic factor (BDNF). The current study was conducted to find out whether BDNF production is impaired in animals devoid of GMF. To this end GMF-knockout (KO) mice were subjected to exercise and the neurotrophin mRNAs were determined by real-time RT-PCR. Compared to wild-type (WT) mice, there is a decrease in exercise-induced BDNF in the KO mice. The observation was correlated with the finding that, in WT mice, exercise increases GMF expression. The results are consistent with the hypothesis that GMF is necessary for exercise-induction of BDNF, and that GMF may promote neuroprotection through BDNF production.
authors.affiliation: Department of Neurology (Division of Neurochemistry and Neurobiology), Veterans Affairs Medical Center and University of Iowa College of Medicine, Iowa City, 52242, USA. asgar-zaheer@uiowa.edu
authors.forename: Asgar
authors.initials: A
authors.lastname: Zaheer
authors.forename: Joel T
authors.initials: JT
authors.lastname: Haas
authors.forename: Carlos
authors.initials: C
authors.lastname: Reyes
authors.forename: Satya N
authors.initials: SN
authors.lastname: Mathur
authors.forename: Baoli
authors.initials: B
authors.lastname: Yang
authors.forename: Ramon
authors.initials: R
authors.lastname: Lim
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020904:Glia Maturation Factor / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D018696:Neuroprotective Agents / Q000378:metabolism; D020933:Neurotrophin 3 / Q000235:genetics / Q000378:metabolism; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020904:Glia Maturation Factor; D018696:Neuroprotective Agents; D020933:Neurotrophin 3; D012333:RNA, Messenger; D020932:Nerve Growth Factor