title: Protein biomarkers in a mouse model of extremes in trait anxiety.
abstract: Brain proteome analysis of mice selectively bred for either high or low anxiety-related behavior revealed quantitative and qualitative protein expression differences. The enzyme glyoxalase-I was consistently expressed to a higher extent in low anxiety as compared with high anxiety mice in several brain areas. The same phenotype-dependent difference was also found in red blood cells with normal and cross-mated animals showing intermediate expression profiles of glyoxalase-I. Another protein that showed a different mobility during two-dimensional gel electrophoresis was identified as enolase phosphatase. The presence of both protein markers in red or white blood cells, respectively, creates the opportunity to screen for their expression in clinical blood specimens from patients suffering from anxiety.
authors.affiliation: Max Planck Institute of Psychiatry, D-80804 Munich, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Ditzen
authors.forename: Archana M
authors.initials: AM
authors.lastname: Jastorff
authors.forename: Melanie S
authors.initials: MS
authors.lastname: Kessler
authors.forename: Mirjam
authors.initials: M
authors.lastname: Bunck
authors.forename: Larysa
authors.initials: L
authors.lastname: Teplytska
authors.forename: Angelika
authors.initials: A
authors.lastname: Erhardt
authors.forename: Simone A
authors.initials: SA
authors.lastname: Krömer
authors.forename: Jeeva
authors.initials: J
authors.lastname: Varadarajulu
authors.forename: Bianca-Sabrina
authors.initials: BS
authors.lastname: Targosz
authors.forename: Eser F
authors.initials: EF
authors.lastname: Sayan-Ayata
authors.forename: Florian
authors.initials: F
authors.lastname: Holsboer
authors.forename: Rainer
authors.initials: R
authors.lastname: Landgraf
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Turck
mesh_terms: D000679:Amygdala / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001007:Anxiety / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism*; D001522:Behavior, Animal; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D015153:Blotting, Western; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000378:metabolism; D008297:Male; D051379:Mice; D010751:Phosphopyruvate Hydratase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D010751:Phosphopyruvate Hydratase; D007791:Lactoylglutathione Lyase