[The viability assessment of ethanol-producing yeast by computer-aided fluorescence microscopy].
Vital staining of the ethanol-producing yeast Saccharomyces cerevisiae with ethidium bromide and DAPI allows intact and damaged cells to be differentiated by fluorescence microscopy. A computer image analysis procedure has been developed for the automatic determination of the relative number of damaged cells using ImageJ software (National Institute of Health, United States; http://rsb.info.nih.gov./ij/). A good correlation has been found between the viability rates determined by the plate count method and the relative numbers of intact cells assessed by the developed procedure in the dry-yeast preparations rehydrated under various conditions.
Puchkov
E O
EO
D015169:Colony Count, Microbial / Q000379:methods*; D000431:Ethanol / Q000378:metabolism*; D004996:Ethidium / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D007211:Indoles / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D013194:Staining and Labeling
D007211:Indoles; D000431:Ethanol; C007293:DAPI; D004996:Ethidium