[Neutralization of hepatitis B virus surface antigen with immunoglobulin preparations].
The in vitro experiments with immunoglobulin and blood plasma containing hepatitis B virus (HBV) markers, revealed that the control of immunoglobulin preparations for the presence of hepatitis B virus surface antigen (HBsAg), mandatory for Russia, was not sufficiently informative. The neutralization of HBsAg with specific antibodies to the level, not determined by the EIA method, reached not less than 24 ng/ ml in 2 hours of incubation and not less than 49 ng/ml in 24 hours of incubation, which, when evaluated in 1 lU of anti-HBs, was 34.6 +/- 0.9 ng and 70.7 +/- 1.8 ng of HBsAg respectively. The process of the formation of immune complexes depended mainly on the time of incubation of experimental samples and on the antibody--antigen proportion in the system. The neutralization of viruses by antibodies had no influence on the capacity of the polymerase chain reaction to detect HBV DNA.
Zubkova
N V
NV
Anastasiev
V V
VV
Moiseeva
M A
MA
Zubov
S V
SV
D000936:Antigen-Antibody Complex; D001688:Biological Products / Q000032:analysis* / Q000592:standards; D006509:Hepatitis B / Q000517:prevention & control; D006510:Hepatitis B Antibodies / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D016756:Immunoglobulins, Intravenous / Q000032:analysis* / Q000276:immunology; D009500:Neutralization Tests / Q000379:methods*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013997:Time Factors
D000936:Antigen-Antibody Complex; D001688:Biological Products; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007106:Immune Sera; D016756:Immunoglobulins, Intravenous