Effect of TNF expression by stromal cells on hemopoietic and stromal precursor cells in long-term bone marrow cultures derived from TNF-deficient mice.
The effect of TNF expression by wild type stromal sublayer on hemopoiesis was studied in cultures after second inoculation of TNF-deficient bone marrow cells. Long-term maintenance of hemopoiesis was determined solely by the absence of autocrine expression of TNF by hemopoietic cells. The production of hemopoietic precursors of all studied types in TNF-deficient cultures on a wild type sublayer was significantly higher than in cultures without TNF production. Presumably, initial expression of TNF in the sublayer promotes the survival of hemopoietic precursors with high proliferative potential in the culture. It is also obvious that TNF is required for normal functioning of stromal precursor cells.
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Hematology Research Center, Russian Academy of Medical Sciences, Moscow.
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D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured / Q000166:cytology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D016130:Immunophenotyping; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D017154:Stromal Cells / Q000378:metabolism*; D013997:Time Factors; D048069:Tumor Necrosis Factors / Q000096:biosynthesis*
D048069:Tumor Necrosis Factors