title: The in vitro effect of a lyophilized extract of wine obtained from Jacquez grapes on human chondrocytes.
abstract: The present work was aimed at evaluating the in vitro effects of a lyophilized extract of wine (JW-E) obtained from Jacquez grapes (Vitis aestivalis-cinereaxVitis vinifera grapes) on the production of key molecules released in inflammatory disease utilising interleukin-1beta (IL-1beta) activated chondrocytes. The extract contains large amounts of phenolic components, in particular some flavonoids (flavan-3-ols, also known as catechins) and proanthocyanidins, as hydroxycinnamic acids and anthocyanins, that possess several biological features such as antiinflammatory and antioxidant effects and a "radical scavenger" activity too. In this study, we assayed the effect of JW-E on the production of key molecules released during chronic inflammatory events as nitric oxide (NO), prostaglandins E(2) (PGE(2)) and reactive oxygen species (ROS) in human chondrocytes culture, stimulated with proinflammatory cytokine interleukin-1beta. The JW-E proved to possess good ability against the harmfull effects of IL-1beta. Our data showed the protective effects of JW-E in cartilage alteration, that appears greater than that elicited by indomethacin, a not steroidal antiinflammatory drug (NSAID), commonly employed in joint diseases.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Catania, V.le A. Doria 6, 95125 Catania, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Panico
authors.forename: V
authors.initials: V
authors.lastname: Cardile
authors.forename: S
authors.initials: S
authors.lastname: Avondo
authors.forename: F
authors.initials: F
authors.lastname: Garufi
authors.forename: B
authors.initials: B
authors.lastname: Gentile
authors.forename: C
authors.initials: C
authors.lastname: Puglia
authors.forename: F
authors.initials: F
authors.lastname: Bonina
authors.forename: N A
authors.initials: NA
authors.lastname: Santagati
authors.forename: G
authors.initials: G
authors.lastname: Ronsisvalle
mesh_terms: D002358:Cartilage, Articular / Q000166:cytology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects*; D015232:Dinoprostone / Q000378:metabolism; D005638:Fruit; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D007375:Interleukin-1 / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D010007:Osteochondritis / Q000188:drug therapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism; D027843:Vitis; D014920:Wine
chemical_list: D007375:Interleukin-1; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D015232:Dinoprostone