title: Effects of bafilomycin A1, a vacuolar type H+ ATPase inhibitor, on the thermosensitivity of a human pancreatic cancer cell line.
abstract: OBJECTIVE
  It has been known that the thermosensitivity of tumour cells can be increased by lowering intra-cellular pH (pHi) by inhibiting pHi control mechanisms. The pHi is partially controlled by transport of H+ from cytoplasm into endocytic and secretary systems in the cells mediated by vacuolar type H+ATPase and also by transport of H+ through plasma membrane.
  
  
  METHODS
  This study investigated the effects the bafilomycine A1, an inhibitor of the vacuolar type H+ATPase and the EIPA, an inhibitor of the Na+/H+ exchanger in plasma membrane, on thermosensitivity of AsPC-1 cells, a human pancreatic cancer cell line. It also investigated the effects of combination of bafilomycine A1 and EIPA.
  
  
  RESULTS
  The treatment of cancer cells with bafilomycine A1 or EIPA individually slightly lowered pHi of the cells in vitro and increased the thermosensitivity of the cells.
  
  
  CONCLUSIONS
  The combination of these two drugs significantly lowered pHi and increased thermosensitivity of cancer cells in vitro and enhanced the heat-induced the growth delay of AsPC-1 tumours grown s.c in the legs of BALB/cA nude mice.
authors.affiliation: First Department of Surgery, Faculty of Medicine, University of Fukui, Matsuoka, Fukui, Japan. yukiho@green.ocn.ne.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Kanji
authors.initials: K
authors.lastname: Katayama
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Togawa
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Kimura
authors.forename: Akio
authors.initials: A
authors.lastname: Yamaguchi
mesh_terms: D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols; D001833:Body Temperature Regulation / Q000187:drug effects* / Q000502:physiology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D003131:Combined Modality Therapy; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006979:Hyperthermia, Induced; D018942:Macrolides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000503:physiopathology* / Q000628:therapy; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D017923:Sodium-Hydrogen Exchangers / Q000037:antagonists & inhibitors; D014183:Transplantation, Heterologous / Q000473:pathology
chemical_list: D004791:Enzyme Inhibitors; D018942:Macrolides; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride; C040929:bafilomycin A1; D006180:Proton-Translocating ATPases; C039614:ethylisopropylamiloride