GMF-knockout mice are unable to induce brain-derived neurotrophic factor after exercise.
We earlier reported that overexpression of glia maturation factor (GMF) in cultured astrocytes enhances the production of brain-derived neurotrophic factor (BDNF). The current study was conducted to find out whether BDNF production is impaired in animals devoid of GMF. To this end GMF-knockout (KO) mice were subjected to exercise and the neurotrophin mRNAs were determined by real-time RT-PCR. Compared to wild-type (WT) mice, there is a decrease in exercise-induced BDNF in the KO mice. The observation was correlated with the finding that, in WT mice, exercise increases GMF expression. The results are consistent with the hypothesis that GMF is necessary for exercise-induction of BDNF, and that GMF may promote neuroprotection through BDNF production.
Zaheer
Asgar
A
Department of Neurology (Division of Neurochemistry and Neurobiology), Veterans Affairs Medical Center and University of Iowa College of Medicine, Iowa City, 52242, USA. asgar-zaheer@uiowa.edu
Haas
Joel T
JT
Reyes
Carlos
C
Mathur
Satya N
SN
Yang
Baoli
B
Lim
Ramon
R
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020904:Glia Maturation Factor / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D018696:Neuroprotective Agents / Q000378:metabolism; D020933:Neurotrophin 3 / Q000235:genetics / Q000378:metabolism; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D020904:Glia Maturation Factor; D018696:Neuroprotective Agents; D020933:Neurotrophin 3; D012333:RNA, Messenger; D020932:Nerve Growth Factor