title: Assessment of oxidative damage induced by acute doses of morphine sulfate in postnatal and adult rat brain.
abstract: The aim of the present study is to evaluate the oxidative damage in rats of different ages. Weaned rats of 25 g and adults of 300 g were used in groups of 6, a single i.p. dose of morphine sulfate of 3, 6 or 12 mg/kg was administered. All animals were sacrificed to measure GSH and 5-HT levels in brain by liquid chromatography, as well as Na(+), K(+)-ATPase and total ATPase enzymatic activity. 5-HT levels decreased significantly (p < 0.05) in adult animals that received 3 and 6 mg morphine. Na(+), K(+)-ATPase activity increased significantly (p < 0.05) in all groups of weaned animals. In adult animals, Na(+), K(+)-ATPase and total ATPase partially diminished. GSH levels diminished significantly (p < 0.05) both in weaned and in adult groups. The results indicate age-induced changes in cellular regulation and biochemical responses to oxidative stress induced by morphine.
authors.affiliation: Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), Mexico DF, Mexico.
authors.forename: David Calderón
authors.initials: DC
authors.lastname: Guzmán
authors.forename: Ivonne Espítia
authors.initials: IE
authors.lastname: Vázquez
authors.forename: Norma Osnaya
authors.initials: NO
authors.lastname: Brizuela
authors.forename: Raquel García
authors.initials: RG
authors.lastname: Alvarez
authors.forename: Gerardo Barragán
authors.initials: GB
authors.lastname: Mejía
authors.forename: Ernestina Hernández
authors.initials: EH
authors.lastname: García
authors.forename: Daniel
authors.initials: D
authors.lastname: Santamaría
authors.forename: Mario la Rosa
authors.initials: Ml
authors.lastname: de Apreza
authors.forename: Hugo Juárez
authors.initials: HJ
authors.lastname: Olguín
mesh_terms: D000367:Age Factors; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009294:Narcotics / Q000494:pharmacology*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D009294:Narcotics; D012701:Serotonin; D009020:Morphine; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione