Induction of BiP by sugar independent of a cis-element for the unfolded protein response in Arabidopsis thaliana.
BiP is a molecular chaperone induced in the unfolded protein response (UPR). In mammalian cells, BiP is induced by glucose starvation when it is called glucose-regulated protein 78 (GRP78). In Arabidopsis thaliana, however, we demonstrated that BiP transcripts decreased with sugar depletion and increased with sugar addition. Transcripts for beta-glucuronidase (GUS) driven by BiP promoter respond to tunicamycin and sugar, being similar with endogenous BiP transcripts in transgenic A. thaliana. When GUS was regulated by P-UPRE, a cis-element responsible for the UPR identified in BiP promoter, GUS transcripts were accumulated by sugar starvation. Subsequently, transgenic A. thaliana harboring luciferase (LUC) gene regulated by P-UPRE was analyzed. Sugar depletion also increased LUC activity. It is concluded that BiP is induced by sugar independent of the cis-element responsible for the UPR.
Tajima
Hiromi
H
Department of Biological Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan.
Koizumi
Nozomu
N
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D002241:Carbohydrates / Q000494:pharmacology*; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D014415:Tunicamycin / Q000494:pharmacology
D029681:Arabidopsis Proteins; D002241:Carbohydrates; D002352:Carrier Proteins; D011994:Recombinant Proteins; D014415:Tunicamycin; C077221:BIP protein, Arabidopsis; D005966:Glucuronidase