title: Neonatal hypothyroidism affects the adenine nucleotides metabolism in astrocyte cultures from rat brain.
abstract: Neonatal hypothyroidism is associated with multiple and severe brain alterations. We recently demonstrated a significant increase in hydrolysis of AMP to adenosine in brain of hypothyroid rats at different ages. However, the origin of this effect was unclear. Considering the effects of adenine nucleotides to brain functions and the harmful effects of neonatal hypothyroidism to normal development of the central nervous system, in this study we investigated the metabolism of adenine nucleotides in hippocampal, cortical and cerebellar astrocyte cultures from rats submitted to neonatal hypothyroidism. ATP and AMP hydrolysis were enhanced by 52 and 210%, respectively, in cerebellar astrocytes from hypothyroid rats. In hippocampus of hypothyroid rats, the 47% increase in AMP hydrolysis was significantly reverted when the astrocytes were treated with T3. Therefore, the imbalance in the ATP and adenosine levels in astrocytes, during brain development, may contribute to some of the effects described in neonatal hypothyroidism.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Elizandra
authors.initials: E
authors.lastname: Braganhol
authors.forename: Alessandra Nejar
authors.initials: AN
authors.lastname: Bruno
authors.forename: Luci
authors.initials: L
authors.lastname: Bavaresco
authors.forename: Maria Luiza M
authors.initials: ML
authors.lastname: Barreto-Chaves
authors.forename: João José Freitas
authors.initials: JJ
authors.lastname: Sarkis
authors.forename: Ana Maria Oliveira
authors.initials: AM
authors.lastname: Battastini
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D007037:Hypothyroidism / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013974:Thyroxine / Q000008:administration & dosage / Q000378:metabolism; D014284:Triiodothyronine / Q000008:administration & dosage / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D014284:Triiodothyronine; D013974:Thyroxine