[Rapid analysis of antiepileptic drugs in human plasma by micellar electrokinetic capillary chromatography].
OBJECTIVE
To develop a rapid and feasible method based on micellar electrokinetic capillary chromatography (MECC) for the simultaneous determination of antiepileptic drugs (AEDs)--phenytoin (PHT), phenobarbital (PB), carbamazepine (CBZ), primidone (PRM) and clonazepam (CZP) in human plasma.


METHODS
Several factors that impact the separation of AEDs with MECC were investigated, such as concentration of sodium dodecyl sulfate (SDS), buffer compositions, pH, organic modifier, internal diameter and temperature, and an optimized MECC running condition was obtained the running buffer consisted of 8 mmol x L(-1) phosphate, 3 mmol x L(-1) sodium tetraborate, and 50 mmol x L(-1) sodium dodecylsulfate (SDS) (pH 8.0), containing acetonitrile (ACN) (18%) as organic modifier. Detection at 210 nm, run at 25 kV at 30 degrees C in a untreated fused silica capillary (50/45.5 cm length, 50 microm ID).


RESULTS
The reproducibility of both migration time and relative peak area with MECC analysis were appropriate for the intra- and inter-assay coefficients. The evaluated drugs concentration intervals of PRM 1.0-40.0 microg x mL(-1), PB 1.0-60.0 microg x mL(-1), PHT 1.0-40.0 microg x mL(-1), CBZ 1.0-40.0 microg x mL(-1), CZP 0.2-8.0 microg x mL(-1) were linear with correlation coefficients higher than 0.999 1, and coefficients of the variation of the points of the calibration curve lower than 10%. The recoveries of AEDs varied from 80.0% to 100.0%, depending on the drug, with coefficients of the variation lower than 10.0%.


CONCLUSIONS
The MECC technique is showed to be rapid, simple, efficient and low cost when applied to monitoring therapeutic drugs in patient treated with a combination of PHT and other AEDs such as hepatic enzyme-inducing agents.
Zhao
Yu
Y
Center for Instrumental Analysis of China Pharmaceutical University, Nanjing.
Rui
Jian-zhong
JZ
Li
Jin-heng
JH
Xiang
Bing-ren
BR
D000927:Anticonvulsants / Q000097:blood*; D002021:Buffers; D002220:Carbamazepine / Q000097:blood; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D002998:Clonazepam / Q000097:blood; D004827:Epilepsy / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010634:Phenobarbital / Q000097:blood; D010672:Phenytoin / Q000097:blood; D011324:Primidone / Q000097:blood; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate
D000927:Anticonvulsants; D002021:Buffers; D011324:Primidone; D002220:Carbamazepine; D012967:Sodium Dodecyl Sulfate; D002998:Clonazepam; D010672:Phenytoin; C028913:dodecyl sulfate; D010634:Phenobarbital