Optimization of microwave-assisted extraction of polycyclic aromatic hydrocarbons from sediments.
The extraction methods for the determination of polycyclic aromatic hydrocarbons (PAHs) from spiked sediment containing benzo[k]fluoranthene, chrysene, acenaphthene, anthracene, carbazole and indeno[1,2,3-c,d]pyrene, using pressurised microwave-assisted extraction (PMAE) and sonication extraction were optimised. Each PAH in the spiked sediment was quantified by using the spectrofluorometric method. The optimised methods were compared on the extraction efficiency of 14 PAHs in a certified sediment reference material, LGC6188. Recoveries of 14 PAHs were performed by HPLC-DAD at 254 nm using the standard addition method. The results showed that the most extraction efficient method was pressurised microwave-assisted extraction with cyclohexane:acetone (3:2) for 15 min at 140% boiling point of acetone. Under this condition for the extracted certified sediment reference material recovery was 96.55%. It can be concluded from the experimental results that pressurised microwave-assisted extraction in a closed system provides a good alternative to sonication extraction for the extraction of PAHs from sediments. Furthermore, the PMAE was also applied to determine PAHs from sediments taken from the main entrance of Kasetsart University on Paholyotin road in Bangkok.
Bangkedphol
Sornnarin
S
Department of Chemistry, Faculty of Science, Kasetsart University, PO Box 1011 Kasetsart, Jatujak, Bangkok, Thailand.
Sakultantimetha
Arthit
A
Keenan
Helen E
HE
Songsasen
Apisit
A
D000096:Acetone / Q000737:chemistry; D003510:Cyclohexanes / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D019015:Geologic Sediments / Q000032:analysis; D008752:Methylene Chloride / Q000737:chemistry; D008872:Microwaves; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000737:chemistry; D013010:Sonication; D013696:Temperature; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000032:analysis*
D003510:Cyclohexanes; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D000096:Acetone; D008752:Methylene Chloride