Locations, abundances, and possible functions of FXYD ion transport regulators in rat renal medulla.
The gamma-subunit of Na-K-ATPase (FXYD2) and corticosteroid hormone-induced factor (CHIF; FXYD4) are considered pump regulators in kidney tubules. The aim of this study was to expand the information about their locations in the kidney medulla and to evaluate their importance for electrolyte excretion in an animal model. The cellular and subcellular locations and abundances of gamma and CHIF in the medulla of control and sodium-depleted rats were analyzed by immunofluorescence and immunoelectron microscopy and semiquantitative Western blotting. The results showed that antibodies against the gamma-subunit COOH terminus and splice variant gamma(a), but not splice variant gamma(b), labeled intercalated cells, but not principal cells, in the initial part of the inner medullary collecting duct (IMCD1). In subsequent segments (IMCD2 and IMCD3), all principal cells exhibited distinct basolateral labeling for both the gamma-subunit COOH terminus, splice variant gamma(a), and CHIF. Splice variant gamma(b) was abundant in the inner stripe of the outer medulla but absent in the inner medulla (IM). Double labeling by high-resolution immunoelectron microscopy showed close structural association between CHIF and the Na-K-ATPase alpha(1)-subunit in basolateral membranes. The present observations provide new information about the cellular and subcellular locations of gamma and CHIF in the renal medulla and show a new gamma variant in the IM. Extensive NaCl depletion did not induce significant changes in the locations or abundances of the gamma-subunit COOH terminus and CHIF in different kidney zones. We conclude that the unchanged levels of these two FXYD proteins suggest that they are not primary determinants for urine electrolyte composition during NaCl depletion.
Pihakaski-Maunsbach
Kaarina
K
The Water and Salt Research Ctr, Institute of Anatomy Univ. of Aarhus, DK-8000 Aarhus, Denmark. Maunsbach@ana.au.dk
Vorum
Henrik
H
Honoré
Bent
B
Tokonabe
Shigeki
S
Frøkiaer
Jørgen
J
Garty
Haim
H
Karlish
Steven J D
SJ
Maunsbach
Arvid B
AB
D000818:Animals; D004039:Diet, Sodium-Restricted; D005455:Fluorescent Antibody Technique; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D007679:Kidney Medulla / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D015221:Potassium Channels / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
C094337:Fxyd4 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D015221:Potassium Channels; D012965:Sodium Chloride; C498817:Fxyd2 protein, rat; D000254:Sodium-Potassium-Exchanging ATPase