Method to obtain C-phycocyanin of high purity.
A new approach is made for the purification of C-phycocyanin (C-PC), which is simple and more efficient than existing methods. The proposed method involves two steps: aqueous two phase extraction and ion-exchange chromatography. Crude extract of C-phycocyanin, of purity 1.18, obtained from Spirulina platensis is subjected to aqueous two phase extraction. C-phycocyanin obtained from this process showed a purity of 5.22, which is higher than the reported value till date. In order to explore the possibility of further purification, C-phycocyanin is subjected to ion-exchange chromatography and found that the purity increased from 5.22 to 6.69. The fluorescence, intactness of structure and purity of C-phycocyanin are confirmed using spectrofluorometry, circular dichroism spectra and sodium dodecyl sulfate-polyacrylamide gel, respectively.
Patil
Ganapathi
G
Department of Food Engineering, Central Food Technological Research Institute, Mysore 570020, India.
Chethana
S
S
Sridevi
A S
AS
Raghavarao
K S M S
KS
D002852:Chromatography, Ion Exchange / Q000379:methods*; D002942:Circular Dichroism; D000458:Cyanobacteria / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D010798:Phycocyanin / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D010798:Phycocyanin