Cloning, in vitro expression and bioactivity of goose interferon-alpha.
The cDNA of goose interferon-alpha (goIFN-alpha) was amplified from PHA stimulated PBMCs of goose by RT-PCR. The cDNA encodes a 30-amino acid signal peptide and a 161-amino acid mature protein, respectively. Recombinant mature goIFN-alpha (rgoIFN-alpha) expressed by prokaryotic and eukaryotic system possessed antiviral activity that was neutralized by rabbit anti-rgoIFN-alpha antibody in vitro. On the other hand, rgoIFN-alpha lacks intrinsic macrophage activating factor (MAF) activity, peripheral blood leukocyte-derived macrophages (PBLMs) could not produce nitric oxide (NO) by stimulate with rgoIFN-alpha as compared to stimulate with recombinant mature goIFN-gamma (rgoIFN-gamma) that was a powerful NO stimulant in vitro.
Li
Hong-Tao
HT
College of Animal Medicine, Northeast Agricultural University, Harbin 150030, China.
Ma
Bo
B
Mi
Jing-Wei
JW
Jin
Hong-Yan
HY
Xu
Li-Na
LN
Wang
Jun-Wei
JW
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005777:Geese / Q000235:genetics* / Q000276:immunology*; D015870:Gene Expression; D016898:Interferon-alpha / Q000096:biosynthesis / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D016898:Interferon-alpha