title: 26RFa, a novel orexigenic neuropeptide, inhibits insulin secretion in the rat pancreas.
abstract: 26RFa is a novel orexigenic neuropeptide identified as the endogenous ligand of the orphan G protein-coupled receptor GPR103. GPR103 shares sequence identity with the receptors for neuropeptide-Y and galanin, two peptides known to inhibit insulin secretion. We have investigated the effect of 26RFa on insulin and glucagon secretion in the perfused rat pancreas. 26RFa dose-dependently reduced glucose-induced insulin release, inhibited the insulin responses to both arginine and exendin-4 and did not affect glucagon output. The inhibitory effect of 26RFa on exendin-4-induced insulin secretion was not observed in pancreata from pertussis toxin-treated rats, thus suggesting that 26RFa may inhibit insulin secretion, at least in part, via a pertussis toxin-sensitive G(i) protein coupled to the adenylyl cyclase system.
authors.affiliation: Hospital Universitario Puerta de Hierro and Department of Physiology, Medical School, Universidad Autónoma de Madrid, San Martín de Porres 4, 28035 Madrid, Spain.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Egido
authors.forename: Raquel
authors.initials: R
authors.lastname: Hernández
authors.forename: Jérôme
authors.initials: J
authors.lastname: Leprince
authors.forename: Nicolas
authors.initials: N
authors.lastname: Chartrel
authors.forename: Hubert
authors.initials: H
authors.lastname: Vaudry
authors.forename: José
authors.initials: J
authors.lastname: Marco
authors.forename: Ramona A
authors.initials: RA
authors.lastname: Silvestre
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007328:Insulin; D009479:Neuropeptides; D005934:Glucagon; D005947:Glucose