[The expression and clinical significance of hematopoietic cell phosphatase and cysteinyl aspartate-specific proteinase in leukemia].
OBJECTIVE
To evaluate the relationship between the expression of hematopoietic cell phosphatase (SHP-1) gene or cysteinyl aspartate-specific proteinases (Caspase-1, 3) gene and the effect of chemotherapy in leukemia patients.


METHODS
The expression of SHP-1 and Caspase-1, 3 gene was measured in leukemia patients with reverse transcriptase-polymerase chain reaction.


RESULTS
The positive rate of SHP-1 and Caspase-1, 3 gene in acute leukemia was 33.3%, 80.0% and 100.0%. The positive rate of SHP-1 in chronic myeloid leukemia (CML)-blastic phase and CML-chronic phase was 0.0%, 37.5%. The first complete remission (CR) rate of SHP-1 and Caspase-1, 3 positive group was 85.0%, 64.3% and 55.8%, which were higher than that of SHP-1 and Caspase-1, 3 negative group (P < 0.05). There was a positive correlation between SHP-1 and Caspase-1, 3.


CONCLUSIONS
SHP-1 gene is potentially an anti-oncogene and may play a role through activation of Caspase-1, 3 gene. The expression of SHP-1 and Caspase-1, 3 is associated with remission rate and it is shown that they participate in the prognosis of leukemia. They could be used as indices for evaluating therapeutic efficacy and judging prognosis in leukemia patients.
Han
Ying
Y
Department of Hematology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Luo
Jian-min
JM
Jia
Xiao-hui
XH
Wang
Fu-xu
FX
Yao
Li
L
Du
Xing-yan
XY
D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000378:metabolism; D020170:Caspase 1 / Q000096:biosynthesis* / Q000235:genetics; D053148:Caspase 3 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D053148:Caspase 3; D020170:Caspase 1