A very efficient bioconversion of soybean phytosterols mixtures to androstanes by mycobacteria.
The production of several high value steroid drugs, used as progestational, adrenocortical, estrogenic and contraceptive agents, is mostly derived from 4-androstene-dione (AD) and 1,4 androsta-diene-3,17-dione (ADD). Three Vietnamese phytosterols mixtures named VN-1, VN-2 and VN-3, isolated from soybean oil may be efficiently converted into these key compounds by mycobacterial cells. Their general phytosterol composition was 55.39, 70.55, 70.19% for VN-1, VN-2 and VN-3, respectively. Moreover, values of campesterol, beta-sitosterol and stigmasterol were determined. After 120 h of shaking in suitable culture media and temperature, maximal yield conversion to ADD was higher than 70% and up to 64% to AD, for the various phytosterols mixtures assays. These results may be better when scaling-up such a procedure of phytosterols conversion.
Pérez
C
C
Department of Biotechnological Processes, National Center for Science Research, PO Box 6990, Havana, Cuba. celso.perez@cnic.edu.cu
Falero
A
A
Duc
H Luu
HL
Balcinde
Y
Y
Hung
B R
BR
D000731:Androstanes / Q000378:metabolism*; D007218:Industrial Microbiology; D009161:Mycobacterium; D010840:Phytosterols / Q000378:metabolism*; D013024:Soybean Oil / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*
D000731:Androstanes; D010840:Phytosterols; D013024:Soybean Oil