[Occurrence of selected genes of the Klebsiella pneumoniae clusters waa and wb for lipolysaccharide synthesis in reference and epidemic strains].
The goal of presented study was to determine by PCR differences in existence or homology level of selected genes involved in K. pneumoniae lipopolysaccharide (LPS) synthesis and application of obtained results for genotyping. Number of 26 reference strains of K. pneumoniae belonging to serogroups O1, O2a, O2a2e, O2a2e2h, O2a2f2g, O3, O4, O5, O7, O8, and O12 was tested together with 13 epidemic strains from 5 outbreaks and 6 casual isolates for the existence of 7 (waaA, waaE, waaL, waaQ, waaZ, waaX and uge) and 4 (wbdA, wbdC, manB, wbbO) genes of the waa and wb clusters for LPS biosynthesis. Based on PCR results, 10 and 11 genotypes were distinguished in tested strains for genes from waa and wb clusters respectively. Derived dendrograms were topologically dissimilar, however observed correlation between clonal groups and O-group was marginal for both compared clusters. Since we aimed to develop genotyping method for K. pneumoniae, genes from clusters waa and wb were used together to enhance the distinguishing capacity. Twenty-one genotypes were distinguished in 45 tested strains (DI=0,46) when 11 genes were applied for typing. Although no apparent correlation between genotype and serogroup was observed, epidemic isolates from 5 outbreaks were diversified into 5 genotypes, whereas strains from the same outbreak were indistinguishable. Described here genotyping method is determinative and was found time and cost effective. This method may be applied in every clinical laboratory equipped in an ordinary PCR apparatus.
Gierczyński
Rafał
R
Zakład Bakteriologii PZH w Warszawie. rgierczynski@pzh.gov.pl
Kałuzewski
Stanisław
S
Zasada
Aleksandra A
AA
Rastawicki
Waldemar
W
Jagielski
Marek
M
D001426:Bacterial Proteins / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D005798:Genes, Bacterial; D005838:Genotype; D005964:Glucosyltransferases / Q000378:metabolism; D006801:Humans; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family; D019081:O Antigens / Q000096:biosynthesis* / Q000235:genetics*; D011044:Poland; D012703:Serotyping / Q000379:methods; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D019081:O Antigens; D005964:Glucosyltransferases; C430063:WaaE protein, Klebsiella pneumoniae