Effects of fluoride on hepatic antioxidant system and transcription of Cu/Zn SOD gene in young pigs.
Thirty-two barrows (Duroc x Landrace x Yorkshire) were randomly divided into four groups, each of which included eight pigs. The groups received the same basal diet supplemented with 0, 100, 250 and 400mg/kg fluoride, respectively. The malondialdehyde (MDA) and glutathione (GSH) levels, antioxidant enzymes activities and zinc/copper superoxide dismutase (Cu/Zn SOD) mRNA content in the liver were determined to evaluate the fluoride hepatic intoxication. Results showed the increased lipid peroxides (LPO) level and the reduced GSH content, along with a concomitant decrease in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px). Moreover, the level of hepatic Cu/Zn SOD mRNA was also significantly reduced. We suggest the mechanism of fluoride injuring the liver as follows: fluoride causes a decrease in Cu/Zn SOD mRNA and the reduced activities of antioxidant enzymes, leads to the declined ability of scavenging free radicals with excessive production of LPO, which seriously damages the hepatic structure and function.
Zhan
Xiu An
XA
College of Animal Science, Feed Science Institute, Zhejiang University, No. 268, Kaixuan Road, HangZhou 310029, PR China.
Wang
Min
M
Xu
Zi Rong
ZR
Li
Wei Fen
WF
Li
Jian Xin
JX
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D005459:Fluorides / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic; D005978:Glutathione / Q000235:genetics / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D014158:Transcription, Genetic
D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D005459:Fluorides