HLA-DR expression, T lymphocyte phenotypes, OKM1 and OKT9 reactive cells in inflammatory myopathy.
Frozen muscle biopsy sections from 13 patients with inflammatory myopathy and from four healthy volunteers were characterized with a double immunohistochemical staining technique, utilizing a panel of antisera to cell surface antigens. The technique enables simultaneous visualization of HLA-DR reactive cells and other cell types, i.e., T lymphocytes. In inflammatory myopathy, large numbers of HLA-DR reactive "macrophage/dendritic" cells were shown. Almost all other inflammatory cells and endothelial cells also expressed HLA-DR, as did the sarcolemma in the vicinity of inflammatory foci. Large numbers of T lymphocytes were detected by alphaLeu-1 antibodies. In most instances, Leu-3a reactive T "helper" lymphocytes dominated over alphaLeu-2a reactive T "suppressor/cytotoxic" lymphocytes. Many T lymphocytes of both phenotypes appeared in close contact with HLA-DR expressing non-T cells. OKM1 and OKT9 antibodies, labeling macrophages and "proliferating" cells, respectively, were common among inflammatory cells. The findings provide basic data that are important for the understanding of inflammatory myopathy.
Olsson
T
T
Department of Neurology, University Hospital Linköping, Sweden.
Henriksson
K G
KG
Klareskog
L
L
Forsum
U
U
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000950:Antigens, Ly / Q000378:metabolism; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016131:Lymphocyte Subsets / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009220:Myositis / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D011990:Receptors, Transferrin / Q000378:metabolism*; D013601:T-Lymphocytes / Q000145:classification / Q000378:metabolism*
D000950:Antigens, Ly; D006684:HLA-DR Antigens; D011990:Receptors, Transferrin