title: Expression and regulation of Nkd-1, an intracellular component of Wnt signalling pathway in the chick embryo.
abstract: The Wnt family of secreted signalling molecules control a wide range of developmental processes in all metazoans. The intracellular response to Wnt signalling depends on the choice of signalling cascade activated in the responding cell. Cells can activate either the canonical pathway that modulates gene expression to control cellular differentiation and proliferation, or the non-canonical pathway that controls cell polarity and movement. Recent work has identified the protein Naked Cuticle to act as an intracellular switch to promote the non-canonical pathway at the expense of the canonical pathway. We have cloned chick Naked Cuticle-1 (cNkd-1) and show that it is expressed in a dynamic manner during early embryogenesis. We show that it is expressed in the somites and in particular regions where cells are undergoing movement. Lastly, we show that the expression of cNkd-1 is regulated by Wnt expression originating from the neural tube. This study provides evidence that non-canonical Wnt signalling plays a part in somite development.
authors.affiliation: Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London, England, NW1 0TU.
authors.forename: Corina
authors.initials: C
authors.lastname: Schmidt
authors.forename: Anthony
authors.initials: A
authors.lastname: Otto
authors.forename: Graham
authors.initials: G
authors.lastname: Luke
authors.forename: Petr
authors.initials: P
authors.lastname: Valasek
authors.forename: William R
authors.initials: WR
authors.lastname: Otto
authors.forename: Ketan
authors.initials: K
authors.lastname: Patel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009672:Notochord / Q000196:embryology / Q000502:physiology; D010802:Phylogeny; D015398:Signal Transduction / Q000502:physiology*; D019170:Somites / Q000502:physiology; D051153:Wnt Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D051153:Wnt Proteins