Regulation of Par-4 by oncogenic Ras.
Oncogenic Ras causes down-regulation of the proapoptotic tumor suppressor gene Par-4. Replenishment of the basal levels of Par-4 results in inhibition of Ras-inducible cellular transformation. Moreover, overexpression of Par-4 (twofold to fourfold over basal levels) results in apoptosis of cells expressing oncogenic Ras. Par-4 does not, on its own, induce apoptosis in immortalized or nontransformed cells. This chapter describes the key methods used for analysis of Par-4 down-regulation by oncogenic Ras, which can be extended to study most genes whose down-regulation by oncogenic Ras is critical for oncogenic transformation and cell survival.
Vasudevan
Krishna Murthi
KM
Dana Farber Cancer Institute, Boston, Massachusetts, USA.
Ranganathan
Padhma
P
Rangnekar
Vivek M
VM
D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D015536:Down-Regulation; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D051379:Mice; D041681:NIH 3T3 Cells; D014162:Transfection; D018631:ras Proteins / Q000502:physiology*
D051017:Apoptosis Regulatory Proteins; C101296:prostate apoptosis response-4 protein; D018631:ras Proteins