[Eyelid conjunctiva can be a potential cells source for conjunctival sack reconstruction purpose].
There is no ideal culture establishing method for the conjunctival sac reconstruction. Two methods of primary conjunctival culture establishing were compared.


METHODS
The internal surface of rat's eyelid was used. In the 1st method cells migrate from specimen and attached to the surface of culture plate. In the 2nd method tissue was digested and placed in the flask. Epithelial character of cultured cells was confirmed.


RESULTS
Both methods allow to set up the culture of epithelial cells. Efficiency of enzymatic isolation was higher then growing from previously attached specimen. At the end of the experiment the cultures were similar.


CONCLUSIONS
Both methods allow to establish the primary culture of conjunctival epithelial cells. Eyelid conjunctiva can be a cells source for the conjunctival sack reconstruction purpose.
Drewa
Tomasz
T
Uniwersytet Mikołaja Kopernika w Toruniu, Collegium Medicum w Bydgoszczy, Zakład Inzynierii Tkankowej, Katedra Biologii Medycznej. tomaszdrewa@wp.pl
Krawczyk
Agnieszka
A
Gałazka
Przemysław
P
Kałuzny
Jakub
J
Sir
Jan
J
D000818:Animals; D021522:Bioartificial Organs / Q000639:trends; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured / Q000166:cytology; D003228:Conjunctiva / Q000166:cytology* / Q000502:physiology / Q000601:surgery*; D004847:Epithelial Cells / Q000637:transplantation*; D005143:Eyelids / Q000166:cytology* / Q000502:physiology; D051381:Rats; D019651:Plastic Surgery Procedures / Q000379:methods*; D023822:Tissue Engineering / Q000639:trends