Evaluation of morphological and molecular variation in Plantago asiatica var. densiuscula, with special reference to the systematic treatment of Plantago asiatica var. yakusimensis.
Morphological and molecular variations in Plantago asiatica L. var. densiuscula Pilg. were analyzed to evaluate the genetic basis for recognizing the dwarf variety P. asiatica var. yakusimensis (Masam.) Ohwi. Considerable variation in the leaf size of P. asiatica var. densiuscula was observed, and no morphological discontinuities were found between the dwarf types of P. asiatica var. densiuscula and P. asiatica var. yakusimensis. Morphological analysis of plants grown under standardized conditions revealed that both environmental plasticity and genetic differentiation contributed to the dwarfisms. Molecular phylogenetic analysis of rDNA internal transcribed spacer (ITS) regions and the SUC1 locus encoding a sucrose transporter revealed that P. asiatica var. yakusimensis was genetically unique although the differentiation level was low. From the above results, we concluded that P. asiatica var. yakusimensis should be reduced to a form of P. asiatica var. densiuscula. Furthermore, the geographic distribution of the SUC1 genotype suggested multiple origins of dwarves, and possible hypotheses for the origins of dwarves are discussed.
Ishikawa
Naoko
N
National Institute for Basic Biology/Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8585, Japan. nakoo@nibb.ac.jp
Yokoyama
Jun
J
Ikeda
Hiroshi
H
Takabe
Eriko
E
Tsukaya
Hirokazu
H
D001483:Base Sequence; D021901:DNA, Intergenic / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D017753:Ecosystem; D014644:Genetic Variation; D026901:Membrane Transport Proteins / Q000235:genetics; D010802:Phylogeny; D018515:Plant Leaves / Q000033:anatomy & histology; D010940:Plant Proteins / Q000235:genetics; D010943:Plantago / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics / Q000502:physiology*; D011003:Ploidies; D012639:Seeds; D013624:Taiwan
D021901:DNA, Intergenic; D018744:DNA, Plant; D026901:Membrane Transport Proteins; D010940:Plant Proteins; C078729:sucrose transport protein, plant