title: [Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer].
abstract: OBJECTIVE
  To study the feasibility of vector-mediated RNA interference for HER-2-positive breast cancer therapy.
  
  
  METHODS
  A plasmid vector capable of mediating HER-2 RNA interference was constructed, and HER-2-positive breast cancer cell line SKBR-3 was transfected with this constructed vector. The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting, and the growth and apoptosis of SKBR-3 cells was analyzed after transfection.
  
  
  RESULTS
  The expressions of HER-2 mRNA and HER-2 protein was downregulated in response to vector-mediated HER-2 RNA interference, which also resulted in tumor cell growth inhibition and increased number apoptotic cells.
  
  
  CONCLUSIONS
  HER-2 is a good target for RNA interference and RNA interference targeting HER-2 can lead to HER-2 breast cancer cell apoptosis and growth inhibition.
authors.affiliation: Center of Breast Cancer, Second Hospital of Sun Yat-sen University, Guangzhou 510272, China.
authors.forename: Xiao-qu
authors.initials: XQ
authors.lastname: Hu
authors.forename: Li
authors.initials: L
authors.lastname: Qin
authors.forename: Feng-xi
authors.initials: FX
authors.lastname: Su
authors.forename: He-rui
authors.initials: HR
authors.lastname: Yao
authors.forename: Ji-sheng
authors.initials: JS
authors.lastname: Chen
authors.forename: Chang
authors.initials: C
authors.lastname: Gong
authors.forename: Ju-jiang
authors.initials: JJ
authors.lastname: Guo
authors.forename: Feng-yan
authors.initials: FY
authors.lastname: Yu
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Jia
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases