title: Chemokine receptor expression and modulation by Mycobacterium tuberculosis antigens on mononuclear cells from human lymphoid tissues.
abstract: Chemokine receptor switching on lymphoid cells is an important factor regulating migration and homing, but little is known about the expression of such molecules during Mycobacterium tuberculosis infection in humans. We describe CCR2, CCR5 and CCR7 expression on human cells from blood, spleen and pulmonary hilar lymph nodes (PHLN) stimulated by M. tuberculosis antigens. CCR2 was not expressed by CD3+ cells regardless of the presence of antigen, but was highly expressed on CD14+ CD63+ monocytes/macrophages. CCR2 decreased on splenic monocytes/macrophages by nearly 50% in culture, independent of antigen, but remained high in blood and PHLN. CCR5 was low in CD3+ cells and was down-regulated by M. tuberculosis antigens on blood and splenic cells but not in PHLN. CCR5 was highly expressed on monocytes/macrophages and was down-regulated by M. tuberculosis antigens at 48 hr only in blood. Less than 15% of CD3+ cells from spleen and PHLN were CCR7+, whereas nearly 40% from blood expressed this receptor on primary isolation. However, CCR7 in PHLN increased in culture, independent of antigen. Monocytes/macrophages did not express CCR7. Thus, we characterize, for the first time, chemokine receptor expression and differential modulation by M. tuberculosis antigens on human mononuclear cells from spleen, blood and PHLN. Knowledge of chemokine receptor switching in human lymphoid tissue provides novel insight into mechanisms of the immune response to M. tuberculosis with potential effects on directing cell trafficking.
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética, Sede de Investigación Universitaria, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia. marias@sgul.ac.uk
authors.forename: Mauricio A
authors.initials: MA
authors.lastname: Arias
authors.forename: Adelis E
authors.initials: AE
authors.lastname: Pantoja
authors.forename: Gabriela
authors.initials: G
authors.lastname: Jaramillo
authors.forename: Sara C
authors.initials: SC
authors.lastname: Paris
authors.forename: Robin J
authors.initials: RJ
authors.lastname: Shattock
authors.forename: Luis F
authors.initials: LF
authors.lastname: García
authors.forename: George E
authors.initials: GE
authors.lastname: Griffin
mesh_terms: D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D002478:Cells, Cultured; D019742:Chemokines, CC / Q000096:biosynthesis; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D008198:Lymph Nodes / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D054390:Receptors, CCR2; D019713:Receptors, CCR5 / Q000378:metabolism; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D060149:Tetraspanin 30
chemical_list: D000942:Antigens, Bacterial; D015703:Antigens, CD; C515334:CCR2 protein, human; C515370:CCR7 protein, human; D017252:CD3 Complex; C554586:CD63 protein, human; D019742:Chemokines, CC; D018950:Lipopolysaccharide Receptors; D010980:Platelet Membrane Glycoproteins; D054390:Receptors, CCR2; D019713:Receptors, CCR5; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D060149:Tetraspanin 30; C072681:Mycobacterium tuberculosis antigens