title: Shear and compression differentially regulate clusters of functionally related temporal transcription patterns in cartilage tissue.
abstract: Chondrocytes are subjected to a variety of biophysical forces and flows during physiological joint loading, including mechanical deformation, fluid flow, hydrostatic pressure, and streaming potentials; however, the role of these physical stimuli in regulating chondrocyte behavior is still being elucidated. To isolate the effects of these forces, we subjected intact cartilage explants to 1-24 h of continuous dynamic compression or dynamic shear loading at 0.1 Hz. We then measured the transcription levels of 25 genes known to be involved in cartilage homeostasis using real-time PCR and compared the gene expression profiles obtained from dynamic compression, dynamic shear, and our recent results on static compression amplitude and duration. Using clustering analysis, we determined that transcripts for proteins with similar function had correlated responses to loading. However, the temporal expression patterns were strongly dependent on the type of loading applied. Most matrix proteins were up-regulated by 24 h of dynamic compression or dynamic shear, but down-regulated by 24 h of 50% static compression, suggesting that cyclic matrix deformation is a key stimulator of matrix protein expression. Most matrix proteases were up-regulated by 24 h under all loading types. Transcription factors c-Fos and c-Jun maximally responded within 1 h to all loading types. Pre-incubating cartilage explants with either a chelator of intracellular calcium or an inhibitor of the cyclic AMP pathway demonstrated the involvement of both pathways in transcription induced by dynamic loading.
authors.affiliation: Biological Engineering Division, Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Fitzgerald
authors.forename: Moonsoo
authors.initials: M
authors.lastname: Jin
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Grodzinsky
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000502:physiology; D002417:Cattle; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology*; D019245:Compressive Strength / Q000502:physiology; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D016133:Polymerase Chain Reaction; D033081:Shear Strength; D013997:Time Factors; D014158:Transcription, Genetic