Cloning, sequence analysis and expression of ovine CD154 (CD40 ligand).
The CD154 (CD40 ligand) molecule is a member of the tumor necrosis factor (TNF) family and plays an important role in the interaction between antigen-specific lymphocytes and antigen-presenting cells. In this study, reverse transcription-PCR cloning was used to derive the sequence encoding ovine CD154. Sequence analysis of the cloned CD154 gene showed a similarity of 97%, 89%, and 88% with the bovine, porcine and human sequences, respectively, at the nucleic acid level. The deduced amino acid sequence for the ovine CD154 shared 97%, 91%, and 87% similarity with the CD154 protein of bovine, porcine and human. The cysteine residues characteristic of the TNF family and N-linked glycosylation sites are conserved although one of the cysteine residues (Cys9) appeared only in ovine CD154. The isolated CD154 sequence was expressed as a mature protein in Chinese hamster ovary (CHO) cells. The analysis of expression of ovine CD154 in mammalian cells by Western blot confirmed the cross reactivity with anti-CD154 antibody.
Oledzka
Gabriela
G
Department of Medical Biology, Medical University of Warsaw, 73 Nowogrodzka, 02-018, Warsaw, Poland.
Li
Bo
B
William Kay
Graham
G
Chomicz
Lidia
L
Stankiewicz
Miroslaw
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D023201:CD40 Ligand / Q000235:genetics* / Q000378:metabolism; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000235:genetics*
D023201:CD40 Ligand