A novel monoclonal antibody with catalytic activity against beta human chorionic gonadotropin.
In this study, we report for the first time, production of monoclonal antibody (MAb) against beta human chorionic gonadotropin (hCG) with proteolytic activity. MAb "7D9" was raised in Balb/C mice using purified human chorionic gonadotropin. Immunoblot analysis and enzyme-linked immunoabsorbent assay (ELISA) showed that this MAb reacts with beta hCG. The epitope for this antibody appears to be located in the C-terminal of beta chain as suggested by the absence of cross-reaction with other glycoprotein hormones such as FSH, TSH and LH. Our data reveal that this MAb is very unstable and has autodegradation characteristics. Zymogram analyses also show that 7D9 MAb has a high level of hydrolytic activity against different substrates such as casein and gelatin. This proteolytic activity can be inhibited by EDTA. These findings demonstrate the proteolytic character of 7D9 MAb and consequently explain its instability.
Mirshahi
Manouchehr
M
Department of Biochemistry, Faculty of Science, Tarbiat Modares University, Tehran, Iran. mirshahi@modares.ac.ir
Shamsipour
Freshteh
F
Mirshahi
Tooran
T
Khajeh
Khosro
K
Naderi-Manesh
Hossein
H
D000818:Animals; D017151:Antibodies, Catalytic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002364:Caseins / Q000276:immunology / Q000378:metabolism; D002384:Catalysis; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000276:immunology*; D006801:Humans; D051379:Mice
D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D002364:Caseins; D018997:Chorionic Gonadotropin, beta Subunit, Human