title: [Study of androgen receptor and phosphoglycerate kinase gene polymorphism in major cellular components of the so-called pulmonary sclerosing hemangioma].
abstract: OBJECTIVE
  To study the clonality of polygonal cells and surface cuboidal cells in the so-called pulmonary sclerosing hemangioma (PSH).
  
  
  METHODS
  17 female surgically resected PSH were found. The polygonal cells and surface cuboidal cells of the 17 PSH cases were microdissected from routine hematoxylin and eosin-stained sections. Genomic DNA was extracted, pretreated through incubation with methylation-sensitive restrictive endonuclease HhaI or HpaII, and amplified by nested polymerase chain reaction for X chromosome-linked androgen receptor (AR) and phosphoglycerate kinase (PGK) genes. The length polymorphism of AR gene was demonstrated by denaturing polyacrylamide gel electrophoresis and silver staining. The PGK gene products were treated with Bst XI and resolved on agarose gel.
  
  
  RESULTS
  Amongst the 17 female cases of PSH, 15 samples were successfully amplified for AR and PGK genes. The rates of polymorphism were 53% (8/15) and 27% (4/15) for AR and PGK genes respectively. Polygonal cells and surface cuboidal cells of 10 cases which were suitable for clonality study, showed the same loss of alleles (clonality ratio = 0) or unbalanced methylation pattern (clonality ratio < 0.25).
  
  
  CONCLUSIONS
  The polygonal cells and surface cuboidal cells in PSH demonstrate patterns of monoclonal proliferation, indicating that both represent true neoplastic cells.
authors.affiliation: Department of Pathology, China Medical University, Shenyang 110001, China.
authors.forename: Feng-jie
authors.initials: FJ
authors.lastname: Qi
authors.forename: Xiu-wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Yong-xing
authors.initials: YX
authors.lastname: Zhang
authors.forename: Shun-dong
authors.initials: SD
authors.lastname: Dai
authors.forename: En-hua
authors.initials: EH
authors.lastname: Wang
mesh_terms: D041321:Chromosomes, Human, X / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D047868:Pulmonary Sclerosing Hemangioma / Q000235:genetics / Q000473:pathology*; D011944:Receptors, Androgen / Q000235:genetics*; D049951:X Chromosome Inactivation
chemical_list: D004273:DNA, Neoplasm; D011944:Receptors, Androgen; D010735:Phosphoglycerate Kinase