[Experimental study of anti-cancer effect of Mannan-modified targeted nanoliposome on mice].
OBJECTIVE
To test the anti-cancer effect of mannan-modified targeted nanoliposome on mice.


METHODS
The 3beta[N-(N',N'-dimethylaminoethane) -carbamoyl] cholesterol (DC-chol) was synthesized by the chemical reaction of cholesteryl chloroformate and N, N-dimethylethylendiamine. The DC-chol was then mixed with dioleoylphosphatidylethanolamine (DOPE) and chol-AECM-mannan to produce the sonicated mannan-modified targeted nanoliposome. The size of the nanoliposome was measured by zetasizer. Nanoliposome or purified EGFR (as control) was injected to the C57 mice with implanted lung cancers intravenously. The growth of tumors was observed, followed by ELISA and Western tests of serum antibodies.


RESULTS
The liposome nanoparticle had a size of 132 nm. The experimental groups had significant higher levels of blood serum antibodies than the controls. The experimental groups also had less volumes of tumors than the controls (P<0.05).


CONCLUSIONS
The growth of tumor has been inhibited by mannan-modified targeted nanoliposome. The mannan-modified targeted nanoliposome has anti-cancer effect on mice.
Yang
Li
L
The Central Teaching Laboratory for Modern Pharmacy, West China Pharmacy School, Sichuan University, Chengdu 610041, China.
Cheng
Li
L
Tian
Ling
L
Fan
Lin-yu
LY
Yin
Hong-mei
HM
Wei
Yu-quan
YQ
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002784:Cholesterol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D004337:Drug Carriers; D016503:Drug Delivery Systems; D066246:ErbB Receptors / Q000008:administration & dosage*; D005260:Female; D008081:Liposomes / Q000008:administration & dosage; D008175:Lung Neoplasms / Q000188:drug therapy*; D008351:Mannans / Q000008:administration & dosage* / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D009368:Neoplasm Transplantation; D011897:Random Allocation
D000970:Antineoplastic Agents; D004337:Drug Carriers; D008081:Liposomes; D008351:Mannans; C488215:N-(2-(cholesteryloxycarbonylamino)ethyl)carbamoylmethylated mannan; D002784:Cholesterol; D066246:ErbB Receptors