title: Cytochemical detection of calcium in a case of calcinosis circumscripta in a dog.
abstract: A 9-month-old intact female Serra-da-Estrela dog (giant Portuguese breed) was presented with a 2-month history of lingual lesions. Two hard, nodular, nonpainful lesions were observed on the lateral border of the tongue. Fine-needle aspiration yielded a chalky white material. Hemacolor-stained smears showed an amorphous granular basophilic background with crystal-like structures and granulomatous inflammation consisting of foamy macrophages and giant cells. Cytochemical staining with von Kossa and Alizarin red S confirmed the calcareous nature of the material. The cytologic diagnosis was lingual calcinosis circumscripta; this was later confirmed by histopathologic examination. To our knowledge, this case is the first to document the use of von Kossa and Alizarin red S on cytologic smears to obtain a definitive diagnosis of lingual calcinosis circumscripta.
authors.affiliation: Cytology Diagnostic Services, Laboratory of Histology and Embryology, ICBAS-Institute of Biomedical Sciences Abel Salazar, University of Porto, Portugal. rmarcos@icbas.up.pt
authors.forename: R
authors.initials: R
authors.lastname: Marcos
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.forename: J
authors.initials: J
authors.lastname: Oliveira
authors.forename: M J
authors.initials: MJ
authors.lastname: Vieira
authors.forename: A L
authors.initials: AL
authors.lastname: Vieira
authors.forename: E
authors.initials: E
authors.lastname: Rocha
mesh_terms: D000818:Animals; D002114:Calcinosis / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D002118:Calcium / Q000032:analysis*; D004283:Dog Diseases / Q000175:diagnosis* / Q000473:pathology; D004285:Dogs; D005260:Female; D006651:Histocytochemistry / Q000662:veterinary; D014060:Tongue Diseases / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*
chemical_list: D002118:Calcium