Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants.
We investigated whether the choice of anticoagulant or the application of density gradient mononuclear cell isolation may account for conflicting published data regarding the levels of the scavenger receptors' expression in healthy individuals. We demonstrate that the detection of CD163, but not CD36, differs dramatically among the methods.
Moniuszko
Marcin
M
Department of Allergology and Internal Medicine, Medical University of Bialystok, Sklodowskiej-Curie Street 24A, 15-276 Bialystok, Poland. moniuszm@amb.edu.pl
Kowal
Krzysztof
K
Rusak
Malgorzata
M
Pietruczuk
Miroslawa
M
Dabrowska
Milena
M
Bodzenta-Lukaszyk
Anna
A
D000293:Adolescent; D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D015703:Antigens, CD / Q000097:blood* / Q000235:genetics; D015214:Antigens, Differentiation, Myelomonocytic / Q000097:blood* / Q000235:genetics; D001773:Blood Cells / Q000187:drug effects* / Q000378:metabolism; D018955:CD36 Antigens / Q000097:blood* / Q000235:genetics; D002452:Cell Count / Q000379:methods; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000097:blood* / Q000235:genetics; D000098902:CD163 Antigen
D000925:Anticoagulants; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D018955:CD36 Antigens; D011956:Receptors, Cell Surface