title: Technical note: a rapid pulsed-field gel electrophoresis method for analysis of bifidobacteria.
abstract: Pulsed-field gel electrophoresis (PFGE) is a widely used and highly discriminatory molecular typing method that has been applied to bifidobacteria. However, published PFGE protocols used with bifidobacteria require between 5 and 7 d to complete. A rapid PFGE method was developed that can be completed within 24 h.
authors.affiliation: Department of Food Science, The Pennsylvania State University, University Park 16802, USA.
authors.forename: E P
authors.initials: EP
authors.lastname: Briczinski
authors.forename: R F
authors.initials: RF
authors.lastname: Roberts
mesh_terms: D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D016133:Polymerase Chain Reaction; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S