title: Microcalorimetry of biological macromolecules.
abstract: The capabilities of contemporary differential scanning and isothermal titration microcalorimetry for studying the thermodynamics of protein unfolding/refolding and their association with partners, particularly target DNA duplexes, are considered. It is shown that the predenaturational changes of proteins must not be ignored in studying the thermodynamics of formation of their native structure and their complexes with partners, particularly their cognate DNA duplexes.
authors.affiliation: Johns Hopkins University, Baltimore, MD 21218, USA. privalov@jhu.edu
authors.forename: Peter L
authors.initials: PL
authors.lastname: Privalov
authors.forename: Anatoly I
authors.initials: AI
authors.lastname: Dragan
mesh_terms: D002151:Calorimetry / Q000379:methods*; D002152:Calorimetry, Differential Scanning / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D006358:Hot Temperature; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D011506:Proteins; D004247:DNA