title: Estrogenic potency of food-packaging-associated plasticizers and antioxidants as detected in ERalpha and ERbeta reporter gene cell lines.
abstract: This study presents the estrogenic potency of 21 food-packaging-associated compounds determined for the first time, using two transfected U2-OS (human osteoblasts devoid of endogenous estrogen receptors) estrogen receptor (ER) alpha and beta cell lines. Six plasticizers and three antioxidants were slightly estrogenic in the ERalpha cells. The model compounds bisphenol A and nonylphenol, one plasticizer [tris(2-ethylhexyl)trimellitate (TEHTM)], and two antioxidants (propyl gallate and butylated hydroxyanisole) were estrogenic in both ERalpha and ERbeta cells. Compared to estradiol (E2), these compounds appeared to be relatively more estrogenic in the ERbeta cells than in the ERalpha cells. Three sorbitol-based plasticizers activated neither ERalpha nor ERbeta and may be good replacements of existing plasticizers. All responses were additive with the response of E2. This indicates that they may contribute to the total effects of the pool of estrogenic compounds humans are exposed to. The estrogenic potencies of these compounds, together with the suggested beneficial effect of ERbeta-mediated responses and adverse ERalpha-mediated effects, support the importance of detecting characteristics for ERalpha and ERbeta response separately in independent models, as done in the present study.
authors.affiliation: Toxicology section, Wageningen University, Tuinlaan 5, 6703 HE Wageningen, The Netherlands. marcel.terveld@wur.nl
authors.forename: Marcel G R
authors.initials: MG
authors.lastname: ter Veld
authors.forename: Bastiaan
authors.initials: B
authors.lastname: Schouten
authors.forename: Jochem
authors.initials: J
authors.lastname: Louisse
authors.forename: Daan S
authors.initials: DS
authors.lastname: van Es
authors.forename: Paul T
authors.initials: PT
authors.lastname: van der Saag
authors.forename: Ivonne M C M
authors.initials: IM
authors.lastname: Rietjens
authors.forename: Albertinka J
authors.initials: AJ
authors.lastname: Murk
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000187:drug effects* / Q000235:genetics; D047629:Estrogen Receptor beta / Q000187:drug effects* / Q000235:genetics; D004967:Estrogens / Q000494:pharmacology*; D018857:Food Packaging; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D010006:Osteoblasts; D010968:Plasticizers / Q000494:pharmacology*; D014162:Transfection
chemical_list: D000975:Antioxidants; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D010968:Plasticizers; D004958:Estradiol