[Analysis of the mitochondrial mutations of articular chondrocyte in temporomandibular joint osteoarthrosis].
OBJECTIVE
To explore the significance of mitochondrial DNA mutations of articular chondrocytes in the rat model of temporomandibular joint osteoarthrosis (TMJOA).


METHODS
TMJOA models were created in left sides of TMJ of 15 SD rats by the partial resection of the articular disc. The experimental rats were killed 3 months after operation. After the chondrocytes culture, the entire mtDNA were amplified using 32 pairs of overlapping. DNA fragments showing different banding patterns between normal and experimental mtDNA by temporal temperature gradient gel electrophoresis were sequenced to identify the mutations.


RESULTS
Of the 35 heteroplasmic pattern PCR products, 42 novel mutations were found, Majority of the novel mutations were in the tRNA and D-loop regions.


CONCLUSIONS
The mutations occurred in the mtDNA of TMJOA articular chondrocytes.
Fang
Ze-qiang
ZQ
Center for TMD & Orofacial Pain, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Ma
Xu-chen
XC
D000818:Animals; D001483:Base Sequence; D002358:Cartilage, Articular / Q000378:metabolism*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D008297:Male; D009154:Mutation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013705:Temporomandibular Joint Disorders / Q000235:genetics* / Q000473:pathology
D004272:DNA, Mitochondrial