title: The differential expression of LCK and BAFF-receptor and their role in apoptosis in human lymphomas.
abstract: OBJECTIVE
  We explored the expression of LCK and BAFF-R (B-cell activating factor receptor) both of which are known to play a role in signaling and apoptosis, in routine tissue biopsies. It was hypothesized that their expression patterns might yield information on apoptosis as it occurs in normal and reactive lymphoid cells, and also be of value for the detection of lymphoma subtypes.
  
  
  METHODS
  Both molecules were studied in paraffin-embedded tissue sections and cell lines by immunoperoxidase staining, and were also studied by western blotting. Human tonsillar B-cell subsets were analyzed by flow cytometry for LCK expression.
  
  
  RESULTS
  LCK was detected for the first time in germinal centers and, at lower levels, in mantle zone B cells. The presence of LCK in B cells was confirmed by western blotting. Cross-linking surface IgM reduced LCK expression whereas cross-linking surface CD40 appeared to have the opposite effect. BAFF-R was present on mantle zone B cells but absent or weakly expressed in germinal center cells. Most lymphomas of germinal center origin (e.g. follicular lymphoma) and also many mantle cell lymphomas, chronic lymphocytic leukemia (CLL) and most T-cell neoplasms expressed LCK. In contrast, BAFF-R was expressed in a variety of B-cell lymphomas, but often absent in grade 3 follicular lymphomas and diffuse large B-cell lymphomas (DLBCL). Both LCK-positive and BAFF-R-positive DLBCL tended to be of germinal-center phenotype.
  
  
  CONCLUSIONS
  The reciprocal expression pattern of LCK and BAFF-R in germinal center and mantle zone B cells may reflect their opposing roles in apoptosis. Their detection in lymphoma tissue biopsies may therefore be of clinical relevance in predicting response to treatment.
authors.affiliation: Leukaemia Research Fund Immunodiagnostics Unit, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford, UK.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Paterson
authors.forename: Sara
authors.initials: S
authors.lastname: Tedoldi
authors.forename: Andrew
authors.initials: A
authors.lastname: Craxton
authors.forename: Margaret
authors.initials: M
authors.lastname: Jones
authors.forename: Martin-Leo
authors.initials: ML
authors.lastname: Hansmann
authors.forename: Graham
authors.initials: G
authors.lastname: Collins
authors.forename: Helen
authors.initials: H
authors.lastname: Roberton
authors.forename: Yasodha
authors.initials: Y
authors.lastname: Natkunam
authors.forename: Stefano
authors.initials: S
authors.lastname: Pileri
authors.forename: Elias
authors.initials: E
authors.lastname: Campo
authors.forename: Edward A
authors.initials: EA
authors.lastname: Clark
authors.forename: David Y
authors.initials: DY
authors.lastname: Mason
authors.forename: Teresa
authors.initials: T
authors.lastname: Marafioti
mesh_terms: D017209:Apoptosis; D053265:B-Cell Activation Factor Receptor; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000235:genetics* / Q000378:metabolism; D008221:Lymphoid Tissue / Q000473:pathology; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000235:genetics; D020522:Lymphoma, Mantle-Cell / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D014066:Palatine Tonsil / Q000276:immunology / Q000473:pathology; D011379:Prognosis; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics* / Q000378:metabolism
chemical_list: D053265:B-Cell Activation Factor Receptor; D008565:Membrane Proteins; D018124:Receptors, Tumor Necrosis Factor; C435926:TNFRSF13C protein, human; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)