title: The pattern of E2F1 and c-myb immunoreactivities in the CA1 region is different from those in the CA2/3 region of the gerbil hippocampus induced by transient ischemia.
abstract: In this study, we examined transient ischemia-induced changes in transcription factor E2F1 and c-myb expressions in the gerbil hippocampus after 5 min of transient forebrain ischemia. E2F1 immunoreactivity significantly increased in the CA1 region 6-12 h after ischemia/reperfusion. c-myb immunoreactivity increased mainly in CA1 pyramidal cells with time by 12 h after ischemia. Thereafter, E2F1 and c-myb immunoreactivities significantly decreased compared to those in the 12 h post-ischemic group. Four days after ischemia/reperfusion, E2F1 and c-myb immunoreactivities were detected in non-pyramidal cells. Ten days after ischemia, c-myb immunoreactivity increased again: at this time, astrocytes as well as non-pyramidal cells showed E2F1 and c-myb immunoreactivities. In the CA2/3 region, E2F1 and c-myb immunoreactivities mainly changed in non-pyramidal cells, and 10 days after ischemia, c-myb immunoreactivity was not expressed in astrocytes. In conclusion, E2F1 and c-myb significantly alter in pyramidal cells and express in astrocytes in the gerbil hippocampal CA1 region after transient ischemia. These results indicate that E2F1 and c-myb in the CA1 region after ischemic damage may be associated with delayed neuronal death.
authors.affiliation: Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, South Korea.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Byung Moon
authors.initials: BM
authors.lastname: Cho
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Hwang
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Kim
authors.forename: Sae Moon
authors.initials: SM
authors.lastname: Oh
authors.forename: Sun Kil
authors.initials: SK
authors.lastname: Choi
authors.forename: Do Yun
authors.initials: DY
authors.lastname: Hwang
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Seung Myung
authors.initials: SM
authors.lastname: Moon
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D016923:Cell Death / Q000502:physiology; D050703:E2F2 Transcription Factor / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology*; D007150:Immunohistochemistry / Q000379:methods; D002546:Ischemic Attack, Transient / Q000473:pathology*; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D020598:Proto-Oncogene Proteins c-myb / Q000378:metabolism*; D015424:Reperfusion; D013997:Time Factors
chemical_list: D050703:E2F2 Transcription Factor; D020598:Proto-Oncogene Proteins c-myb