Use of self-quenched, fluorogenic LUX primers for gene expression profiling.
Application of a real-time detection system based on a novel primer design in gene expression profiling is described. In this system, called LUX (Light Upon eXtension), the generation of signal is based on a single fluorescent dye molecule that is attached to an oligonucleotide close to the 3'-end. A primer design software is available that identifies LUX primer pairs based on a set of rules for optimum signal development. The use of LUX fluorogenic primers to determine the expression patterns of various transcripts during differentiation in the P-19 mouse neuronal model is described.
Kusser
Wolfgang
W
Research and Development, Invitrogen Corporation, Carlsbad, CA, USA.
D000818:Animals; D002460:Cell Line; D017931:DNA Primers; D005456:Fluorescent Dyes; D020869:Gene Expression Profiling / Q000379:methods*; D051379:Mice; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D005456:Fluorescent Dyes; D015335:Molecular Probes