Effect of insulin on Ca(2+)-dependent hyperpolarization in erythrocytes from healthy donors and patients with type 2 diabetes mellitus accompanied by arterial hypertension.
Insulin decreased A23187-induced hyperpolarization of the erythrocyte membrane in healthy donors. These data indicate that insulin plays a role in the regulation of Ca(2+)-activated potassium channels in human erythrocytes. However, insulin had little effect on hyperpolarization response of cells induced by artificial ascorbate--phenazine methosulfate donor-acceptor system. Addition of insulin to cell suspension from patients with type 2 diabetes mellitus did not modulate hyperpolarization of the erythrocyte membrane induced by A23187 or ascorbate-phenazine methosulfate, which reflects impairment of regulatory mechanisms for Ca(2+)-activated potassium channels in erythrocytes.
Kremeno
S V
SV
Institute of Cardiology, Tomsk Research Center, Siberian Division of the Russian Academy of Medical Sciences. ksv@cardio.tsu.ru
Sitozhevskii
A V
AV
Petrova
I V
IV
Starikova
N S
NS
Karpov
R S
RS
D001158:Arteries / Q000473:pathology*; D001205:Ascorbic Acid / Q000737:chemistry; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006973:Hypertension / Q000473:pathology*; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology; D010084:Oxidation-Reduction
D007328:Insulin; D007476:Ionophores; D008773:Methylphenazonium Methosulfate; D000001:Calcimycin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D001205:Ascorbic Acid; D002118:Calcium