Labelling of human mesenchymal stem cells with indium-111 for SPECT imaging: effect on cell proliferation and differentiation.
OBJECTIVE
Stem cell therapy seems to be a new treatment option within cardiac diseases to improve myocardial perfusion and function. However, the delivery and traceability of the cells represent a problem. Radioactive labelling with 111In could be a method for tracking mesenchymal stem cells (MSCs). However, 111In could influence the viability and differentiation capacity of MSCs, which would limit its use. Therefore, the aim of this study was to evaluate the influence of 111In labelling in doses relevant for SPECT imaging in humans on the viability and differentiation capacity of human MSCs.


RESULTS
Human MSCs isolated from bone marrow were incubated with 111In-tropolone (15-800 Bq/cell). The labelling efficiency was approximately 25% with 30 Bq/cell 111In. The MSC doubling time was 1.04+/-0.1 days and was not influenced by 111In within the range 15-260 Bq/cell. Using 30 Bq 111In/cell it was possible to label MSCs to a level relevant for clinical scintigraphic use. With this dose, 111In had no effect on characteristic surface and intracellular markers of cultured MSCs analysed both by flow cytometry and by real-time polymerase chain reaction. Further, the labelled MSCs differentiated towards endothelial cells and formed vascular structures.


CONCLUSIONS
It is possible to label human MSCs with 111In for scintigraphic tracking of stem cells delivered to the heart in clinical trials without affecting the viability and differentiation capacity of the MSCs. This creates an important tool for the control of stem cell delivery and dose response in clinical cardiovascular trials.
Bindslev
L
L
Stem Cell Laboratory 9312, The Heart Centre, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
Haack-Sørensen
M
M
Bisgaard
K
K
Kragh
L
L
Mortensen
S
S
Hesse
B
B
Kjaer
A
A
Kastrup
J
J
D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000000981:diagnostic imaging* / Q000187:drug effects; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000000981:diagnostic imaging*; D009942:Organometallic Compounds / Q000008:administration & dosage*; D019275:Radiopharmaceuticals / Q000008:administration & dosage; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*; D014334:Tropolone / Q000008:administration & dosage / Q000031:analogs & derivatives*
D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; C043895:indium tris(tropolonate); D014334:Tropolone