title: Relationship between haptoglobin, serum amyloid A, and clinical status in a survey of dairy herds during a 6-month period.
abstract: BACKGROUND
  Haptoglobin and serum amyloid A are major acute phase proteins in cattle. Dairy cattle often develop pathologic conditions in the peripartum period; acute phase proteins may be useful in their diagnosis.
  
  
  OBJECTIVE
  The purpose of this study was to compare the accuracy of serum haptoglobin (Hp) and serum amyloid A (SAA) concentrations with clinical health status for diagnosing disease during the peripartum period in dairy cattle.
  
  
  METHODS
  Dairy cows from 4 herds were evaluated every 15 days over a 6-month period. Health status was determined by thorough clinical examination. Haptoglobin and SAA concentrations were measured in serum using validated methods and the results were classified as positive or negative based on defined cutoff points. Disease prevalence, sensitivity, and specificity were compared using clinical examination as the gold standard.
  
  
  RESULTS
  A total of 1896 samples from 158 cows were analyzed. Significant increases in mean Hp and SAA concentrations were observed in the week following parturition in both primiparous and multiparous cows, although high interindividual variability was observed. Both Hp and SAA had low sensitivity but higher specificity in determining disease status compared with clinical examination. Increased concentrations of Hp and SAA were found in <10% of samples from clinically healthy cows, except in the week after parturition.
  
  
  CONCLUSIONS
  Haptoglobin and serum amyloid A should be used with caution as markers of inflammation in the week after calving. Poor sensitivity in other postpartum periods could be related to the higher incidence of chronic (vs acute) inflammation. Haptoglobin may be appropriate for routine screening, but further work needs to be done to assess its value as an indicator of herd health.
authors.affiliation: Department of Functional Sciences, Unit of Biochemistry, Faculty of Veterinary Medicine, University of Liege, 4000 Liege-SartTilman, Belgium. mfhumblet@ulg.ac.be
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Humblet
authors.forename: Hugues
authors.initials: H
authors.lastname: Guyot
authors.forename: Benjamin
authors.initials: B
authors.lastname: Boudry
authors.forename: Faustin
authors.initials: F
authors.lastname: Mbayahi
authors.forename: Christian
authors.initials: C
authors.lastname: Hanzen
authors.forename: Frederic
authors.initials: F
authors.lastname: Rollin
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Godeau
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D003625:Data Collection; D005260:Female; D006242:Haptoglobins / Q000378:metabolism*; D012680:Sensitivity and Specificity; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D013997:Time Factors
chemical_list: D006242:Haptoglobins; D000685:Serum Amyloid A Protein