Association of platelet-activating factor acetylhydrolase gene polymorphism with premature coronary artery disease in Turkish patients.
OBJECTIVE
Platelet-activating factor (PAF) is a phospholipid with multiple actions that is involved in inflammatory diseases as well as in atherogenesis. It is inactivated by a plasma enzyme, PAF-acetylhydrolase (PAF-AH). Deficiency of this enzyme in plasma is caused by a missense mutation in the gene (G994T). The aim of this study was to investigate association of this mutation with premature coronary artery disease (CAD).


METHODS
One hundred and fifteen unrelated Turkish patients with a diagnosis of premature CAD and 128 unrelated healthy subjects were enrolled in this study. Genotyping was performed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).


RESULTS
The prevalence of the G994T mutation in the patients was 2.60 % (heterozygote), and 0 % in the controls. There was no significant difference in allele frequency and genotype distribution among the study groups.


CONCLUSIONS
The G9943T mutation in the plasma PAF acetylhydrolase gene is not associated with premature CAD in Turkish subjects.
Sekuri
Cevad
C
Department of Cardiology Faculty of Medicine, Celal Bayar University, Manisa, Turkey. csekuri@hotmail.com
Cam
F Sirri
FS
Tengiz
Istemihan
I
Ercan
Ertuğrul
E
Bayturan
Ozgür
O
Berdeli
Afig
A
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000235:genetics*; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood / Q000453:epidemiology* / Q000209:etiology / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D014421:Turkey / Q000453:epidemiology; D044465:White People / Q000235:genetics
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase