title: Production and partial characterization of dehairing protease from Bacillus cereus MCM B-326.
abstract: Bacillus cereus MCM B-326, isolated from buffalo hide, produced an extracellular protease. Maximum protease production occurred (126.87+/-1.32 U ml(-1)) in starch soybean meal medium of pH 9.0, at 30 degrees C, under shake culture condition, with 2.8 x 10(8) cells ml(-1) as initial inoculum density, at 36 h. Ammonium sulphate precipitate of the enzyme was stable over a temperature range of 25-65 degrees C and pH 6-12, with maximum activity at 55 degrees C and pH 9.0. The enzyme required Ca(2+) ions for its production but not for activity and/or stability. The partially purified enzyme exhibited multiple proteases of molecular weight 45 kDa and 36 kDa. The enzyme could be effectively used to remove hair from buffalo hide indicating its potential in leather processing industry.
authors.affiliation: Microbial Sciences Division, Agharkar Research Institute, G.G. Agarkar Road, Pune, Maharashtra State 411004, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Nilegaonkar
authors.forename: V P
authors.initials: VP
authors.lastname: Zambare
authors.forename: P P
authors.initials: PP
authors.lastname: Kanekar
authors.forename: P K
authors.initials: PK
authors.lastname: Dhakephalkar
authors.forename: S S
authors.initials: SS
authors.lastname: Sarnaik
mesh_terms: D001409:Bacillus cereus / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D010447:Peptide Hydrolases