title: A high-performance liquid chromatography method for hypoxanthine determination in vitreous humour: application to estimation of post mortem interval.
abstract: We have developed a new technique to determine the concentration of hypoxanthine [Hx] in a reverse phase column using a modified high-performance liquid chromatography (HPLC) method that is faster and more reliable than those previously described. In this paper we present a formula for estimating the post mortem interval (PMI) based on this HPLC method by applying the inverse prediction method. The regression line obtained by changing the variables gives PMI = 0.183 [Hx] + 0.599 (PMI in hours, [Hx] in micromol/L, R2 = 0.531, P < 0.05).
authors.affiliation: Department of Pathology and Forensic Sciences, University of Santiago de Compostela, Spain.
authors.forename: J I
authors.initials: JI
authors.lastname: Muñoz
authors.forename: E
authors.initials: E
authors.lastname: Costas
authors.forename: M S
authors.initials: MS
authors.lastname: Rodríguez-Calvo
authors.forename: J M
authors.initials: JM
authors.lastname: Suárez-Peñaranda
authors.forename: M
authors.initials: M
authors.lastname: López-Rivadulla
authors.forename: L
authors.initials: L
authors.lastname: Concheiro
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005554:Forensic Medicine; D006801:Humans; D007042:Hypoxanthines / Q000032:analysis*; D011180:Postmortem Changes; D015203:Reproducibility of Results; D014822:Vitreous Body / Q000737:chemistry*
chemical_list: D007042:Hypoxanthines