title: An 8 nt RNA triggers a rate-limiting shift of RNA polymerase II complexes into elongation.
abstract: To better understand the critical conversions that RNA polymerase II complexes undergo during promoter escape, we determined in vitro the precise positions of the rate-limiting step and the last step requiring negative superhelicity or TFIIE and TFIIH. We found that both steps occur after synthesis of an 8 nt RNA during the stage encompassing translocation of the polymerase active site to the ninth register. When added to reactions just before this step, TFIIE and TFIIH overcame the requirement for negative superhelicity. The positions at which both steps occur were strictly dependent on RNA length as opposed to the location of the polymerase relative to promoter elements, showing that the transcript itself controls transformations during promoter escape. We propose a model in which completion of promoter escape involves a rate-limiting conversion of early transcribing complexes into elongation complexes. This transformation is triggered by synthesis of an 8 nt RNA, occurs independent of the general transcription factors, and requires under-winding in the DNA template via negative superhelicity or the action of TFIIE and TFIIH.
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado at Boulder, 80309-0215, USA.
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Hieb
authors.forename: Sean
authors.initials: S
authors.lastname: Baran
authors.forename: James A
authors.initials: JA
authors.lastname: Goodrich
authors.forename: Jennifer F
authors.initials: JF
authors.lastname: Kugel
mesh_terms: D006801:Humans; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D012319:RNA Polymerase II / Q000235:genetics / Q000502:physiology*; D013698:Templates, Genetic; D051758:Transcription Factor TFIIH / Q000235:genetics / Q000502:physiology*; D019473:Transcription Factors, TFII / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D019473:Transcription Factors, TFII; C066644:transcription factor TFIIE; D051758:Transcription Factor TFIIH; D012313:RNA; D012319:RNA Polymerase II