title: Evolutionary breakpoints through a high-resolution comparative map between porcine chromosomes 2 and 16 and human chromosomes.
abstract: This study reports a high-resolution comparative map between human chromosomes and porcine chromosomes 2 (SSC2) and 16 (SSC16), pointing out new homologies and evolutionary breakpoints. SSC2 is of particular interest because of the presence of several important QTLs. Among 226 porcine ESTs selected according to their expected localization, 151 were RH mapped and ordered on SSC2. This study confirmed the extensive conservation between SSC2 and HSA11 and HSA19 and refined the homology with HSA5 (three blocks defined). Furthermore the SSC2q pericentromeric region was shown to be homologous to another human chromosome (HSA1). A complex organization of these syntenies was demonstrated on SSC2q. Our strategy led us to improve also the SSC16 RH map by adding 45 markers. Two-color fluorescence in situ hybridization of markers representative of each synteny confirmed block order. Finally, 29 breakpoints were identified in both species, and porcine BACs containing two breakpoints were isolated.
authors.affiliation: Institut National de la Recherche Agronomique, Laboratoire de Génétique Cellulaire, BP52627, 31326 Castanet-Tolosan, France. lahbib@toulouse.inra.fr
authors.forename: Yvette
authors.initials: Y
authors.lastname: Lahbib-Mansais
authors.forename: Florence
authors.initials: F
authors.lastname: Mompart
authors.forename: Denis
authors.initials: D
authors.lastname: Milan
authors.forename: Sophie
authors.initials: S
authors.lastname: Leroux
authors.forename: Thomas
authors.initials: T
authors.lastname: Faraut
authors.forename: Chantal
authors.initials: C
authors.lastname: Delcros
authors.forename: Martine
authors.initials: M
authors.lastname: Yerle
mesh_terms: D000818:Animals; D005075:Biological Evolution; D019457:Chromosome Breakage; D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial; D002877:Chromosomes, Human; D033481:Chromosomes, Mammalian / Q000235:genetics*; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D023281:Genomics / Q000379:methods; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D013552:Swine / Q000235:genetics*
chemical_list: D005819:Genetic Markers