Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste.
The aim of this work was to study chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. The culture produced two biosurfactants, a and b, which showed strong activity and were identified as L-rhamnopyranosyl-L-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydecanoate or Rha-Rha-C10-C10 and L-rhamnopyranosyl-L-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydodecanoate or Rha-Rha-C10-C12, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as SDS and Tween 80. Rhamnolipid a showed significant antiproliferative activity against human breast cancer cell line (MCF-7) at minimum inhibitory concentration (MIC) at 6.25 microg/mL while rhamnolipid b showed MIC against insect cell line C6/36 at 50 microg/mL.
Thanomsub
Benjamas
B
Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand. benjamat@swu.ac.th
Pumeechockchai
Wanna
W
Limtrakul
Anirut
A
Arunrattiyakorn
Panarat
P
Petchleelaha
Wipawan
W
Nitoda
Teruhiko
T
Kanzaki
Hiroshi
H
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005525:Food-Processing Industry; D006017:Glycolipids / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007218:Industrial Microbiology; D007313:Insecta; D008826:Microbial Sensitivity Tests; D008892:Milk; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000378:metabolism*; D013501:Surface-Active Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D014865:Waste Disposal, Fluid
D000970:Antineoplastic Agents; D006017:Glycolipids; D013501:Surface-Active Agents; C418382:rhamnolipid