Surgical bone marrow aspiration in Aotus lemurinus griseimembra.
Aotus lemurinus griseimembra are highly susceptible to infection by human malaria parasites and reproduce some of its clinical manifestations, including anemia. We developed a new surgical technique to obtain bone marrow samples from Aotus by surgical aspiration of the femur. First, we determined that the femur offered advantages over other bones, primarily due to lower fracture vulnerability. We tested a surgical technique using 20 G IV catheters in formaldehyde-preserved animals, then conducted the procedure on 27 live animals. This technique provided easy, quick surgical access to adequate volumes of bone marrow and was safe for almost all animals: only one died; another developed nervous impairment of the lower limb. Adequate cell samples were obtained in all animals and allowed cytological studies. This procedure offers a useful tool for bone marrow research in Aotus and helps overcome current limitations of such research in human where these studies are limited by ethical and technical issues.
Llanos
Cesar
C
Immunology Institute, Universidad del Valle, AA 25574 Cali, Colombia.
Quintero
Gustavo
G
Castellanos
Alejandro
A
Arevalo-Herrera
Myriam
M
Herrera
Sòcrates
S
D000818:Animals; D016645:Aotidae / Q000601:surgery*; D044963:Biopsy, Fine-Needle / Q000379:methods* / Q000662:veterinary*; D001853:Bone Marrow / Q000601:surgery*; D005260:Female; D005269:Femur / Q000601:surgery; D008297:Male