Transgenic RNAi: Accelerating and expanding reverse genetics in mammals.
Reverse genetics in mammals has relied on gene targeting strategies and has mostly been limited to the mouse. Gene targeting through homologous recombination in mouse ES cells has drawbacks which include time, expense and complexity. Recently, a new approach has been developed based on RNA-interference (RNAi) which is simpler, faster, less expensive, and should be applicable to mammalian species other than mouse. The advent of RNAi is poised to accelerate the pace at which reverse genetics can be applied to study gene function in mammals.
Xia
Xu-Gang
XG
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA. Xugang.xia@umassmed.edu
Zhou
Hongxia
H
Xu
Zuoshang
Z
D000818:Animals; D004257:DNA Polymerase II / Q000378:metabolism; D004258:DNA Polymerase III / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology; D020868:Gene Silencing; D018390:Gene Targeting; D005821:Genetic Techniques; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D034622:RNA Interference; D011995:Recombination, Genetic; D019076:Transgenes
D004257:DNA Polymerase II; D004258:DNA Polymerase III