title: Identification of AGE-modified proteins in SH-SY5Y and OLN-93 cells.
abstract: The formation of "Advanced Glycation End products" (AGEs) is an inevitable consequence of mammalian glucose metabolism. AGE-mediated protein-protein crosslinks lead to detergent-insoluble and protease-resistant protein aggregates, and in Alzheimer's disease (AD) extra cellular senile plaques (SPs) and intracellular neurofibrillary tangles (NFTs) have been shown to contain AGEs. However, to date little is known concerning the most prevalent protein-targets of AGE modification under normal, non-pathological conditions. Here, a combination of 2D-electrophoresis, Western blotting and mass spectrometry has been used to identify preferentially AGE-modified proteins in oligodendrocyte (OLN-93) and neuroblastoma cell lines (SH-SY5Y) in standard culture. Proteomics analysis identified a total of eight targets with structural, metabolic and regulatory function, three of which (beta-actin, beta-tubulin and eukaryotic Elongation Factor 1-alpha) were common to both cell lines. Based on results from prior studies, modification of these proteins may lead to a loss of function. Consequently, the identification of targets for these proteins is of particular interest for a better understanding of the consequences of AGE-modification in aging, neurodegenerative diseases and diabetes.
authors.affiliation: Nachwuchsgruppe 1, Interdisciplinary Centre of Clinical Research (IZKF), University of Leipzig, 04103 Leipzig, Germany. Andre.Langer@web.de
authors.forename: André K
authors.initials: AK
authors.lastname: Langer
authors.forename: H Fai
authors.initials: HF
authors.lastname: Poon
authors.forename: Gerald
authors.initials: G
authors.lastname: Münch
authors.forename: Bert C
authors.initials: BC
authors.lastname: Lynn
authors.forename: Thomas
authors.initials: T
authors.lastname: Arendt
authors.forename: D Allan
authors.initials: DA
authors.lastname: Butterfield
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002682:Chinchilla; D017127:Glycation End Products, Advanced / Q000032:analysis* / Q000096:biosynthesis*; D006801:Humans; D009836:Oligodendroglia / Q000737:chemistry* / Q000378:metabolism; D011817:Rabbits; D051381:Rats
chemical_list: D017127:Glycation End Products, Advanced