title: A novel mammalian bubblegum-related acyl-CoA synthetase restricted to testes and possibly involved in spermatogenesis.
abstract: We have characterized a new, membrane-associated acyl-CoA synthetase (ACS), termed bubblegum-related protein (BGR), which upon functional analysis demonstrated ACS activity capable of activating long- and very long-chain fatty acids. By multiple tissue RNA array and Northern blot analyses, human BGR mRNA was exclusively detected in testes. Murine Bgr mRNA was specifically expressed in pubertal and adult testes and was further demonstrated to be enriched in germ cells and Sertoli cells while present at a lower level in Leydig cells both by in situ hybridization and cell type fractionation. The complex 5'-end of the BGR mRNA appears to underlie translational control leading to differential utilization of alternative translation start sites. Thus, the BGR gene expands the bubblegum ACS family with a testes-specific, developmentally regulated member that may play a role in spermatogenesis.
authors.affiliation: Center for Brain Research, Division of Neuroimmunology, Medical University of Vienna, Spitalgasse 4, A-1090 Vienna, Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Fraisl
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Tanaka
authors.forename: Sonja
authors.initials: S
authors.lastname: Forss-Petter
authors.forename: Hans
authors.initials: H
authors.lastname: Lassmann
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Nishimune
authors.forename: Johannes
authors.initials: J
authors.lastname: Berger
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D019556:COS Cells / Q000378:metabolism; D002522:Chlorocebus aethiops; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D005854:Germ Cells / Q000378:metabolism; D006801:Humans; D007985:Leydig Cells / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012708:Sertoli Cells / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology*; D013737:Testis / Q000201:enzymology* / Q000378:metabolism; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
chemical_list: D012333:RNA, Messenger; D003066:Coenzyme A Ligases; C418138:lipidosin; D000098859:Long-Chain-Fatty-Acid-CoA Ligase