[In vivo fluorescent microscopy of the liver microcirculation after embolization with a newly developed trans-proper hepatic arterial infusion animal model].
OBJECTIVE
To evaluate the value of the in vivo fluorescent microscopy in studying the changes of liver microcirculation after embolization with a newly developed animal model for tans-proper hepatic arterial infusion, and to summarize the method of making this animal model.


METHODS
Ten Sprague-Dawley rats were used in this study. After a midline abdominal incision, microcatheter was placed into the gastroduodenal artery (GDA). The tip of the catheter was placed facing the orifice of proper hepatic artery. After infusions of 0.02%, 0.1%, 0.5%, 1% fluorescent sodium, fluorescent microscopy was used to evaluate the liver microcirculation. The image quality was then accessed. Embolization was obtained by injections of Lipiodol and Degradable Starch Microspheres (DSM) from the microcatheter. Corresponding changes of the liver microcirculation was evaluated by fluorescent microscopy.


RESULTS
From the 10 rats, 8 animal models were successfully established. The microcirculation of the liver could be clearly visualized by the fluorescent microscopy. The optimal concentration of fluorescent sodium was 0.1%. The direct and indirect phenomena caused by embolic material could be evaluated by fluorescent microscopy.


CONCLUSIONS
Fluorescent microscopy with the corresponding Trans-hepatic arterial infusion animal model is a valuable method to evaluate the microcirculation of the liver and can be used for the evaluation of changes of liver microcirculation caused by embolization material.
Wang
Jian
J
Department of Interventional Radiology and Vascular Surgery, Peking University First Hospital, Beijing 100034, China.
Zou
Ying-hua
YH
Tong
Xiao-qiang
XQ
Lv
Yong-xing
YX
Jiang
Xue-xiang
XX
D000818:Animals; D004621:Embolization, Therapeutic / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage; D006499:Hepatic Artery / Q000502:physiology*; D007261:Infusions, Intra-Arterial; D008099:Liver / Q000098:blood supply*; D008297:Male; D008833:Microcirculation; D008856:Microscopy, Fluorescence / Q000379:methods*; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000008:administration & dosage*
D005456:Fluorescent Dyes; C083286:degradable starch microspheres; D013213:Starch