[A study on the protective efficacy of magnesium against anoxic damage to cultured neurons of rats].
OBJECTIVE
This is an in vitro study aimed to assess the influence of magnesium on cultured anoxic cortical neurons of rats.


METHODS
After being treated with MgSO4 and Mg(2+)-ATP respectively, the cortical neurons of rats were given mixture of 97.5% N2 and 2.5% CO2 continuously to set up the model of anoxic cortical neuron of rats at different time points. The mortality of neurons, the levels of Ca2+ within nerve cells (marked by Fluo-3, detected by laser confocal microscopy), and the levels of lactate dehydrogenase (LDH), K+ and neuron specific enolase (NSE) in the culture fluid were investigated at 1,2,4 hours after the inception of anoxia.


RESULTS
MgSO4 and Mg(2+)-ATP reduced the calcium influx of anoxic cortical neuron (P<0.05), lowered the level of LDH, K+, NSE in the culture fluid (P<0.05) and significantly decreased the mortality of neurons (P <0.05).


CONCLUSIONS
The protective efficacy of magnesium against the anoxic damage to cultured cortical neurons of rats is corroborated.
Zheng
Hong-bo
HB
Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
Luo
Zu-ming
ZM
Yang
Jie
J
Zou
Xiao-yi
XY
Kong
Shuang-Yan
SY
Zhang
Jie
J
Zeng
Hong
H
He
Li
L
D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000473:pathology*; D008274:Magnesium / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018696:Neuroprotective Agents; D008274:Magnesium