title: [Research of the spermatozoal gene expression with gene microarrays].
abstract: OBJECTIVE
  To perform the detection of spermatozoal gene expression in order to accelerate the study of spermatozoal molecular biology.
  
  
  METHODS
  To collect the healthy adults sperm and lymphocytes respectively, and then to extract the total RNAs from them by RNeasy mini kit (QIAGEN) or Trizol reagent. Corresponding cDNAs were produced, digested, ligated, finally labeled with Cy3 (sperm) and CyS (lymphocyte) in the course of RD amplifying reactions. Hybridization with self-made microarrays contained 560 probes was carried out after the labeled cDNAs pured by PCR Product Purification Kit.
  
  
  RESULTS
  Among the 560 probes, 72 genes were up-regulated, 321 genes were down-regulated, the others had no different expression. Furthermore, genes associated with replication, transcription, translation and regulative functions were non-different expression or down-regulated, and those belonged to the spermatogenesis associated, sperm associated antigen were up-regulated, but those involved in the glycolysis were up-regulated, in the oxidative phosphorylation were down-regulated.
  
  
  CONCLUSIONS
  It had successfully confirmed that there were a plenty of genes expressed in sperm, furthermore the genes expressed were accorded to spermatozoal functions and characteristics.
authors.affiliation: Department of Urology, Nanfang Hospital, Guangzhou, Guangdong 510515, China. mxm@fimmu.com
authors.forename: Xiang-Ming
authors.initials: XM
authors.lastname: Mao
authors.forename: Chun-Qiong
authors.initials: CQ
authors.lastname: Feng
authors.forename: Ya-Guang
authors.initials: YG
authors.lastname: Zou
authors.forename: Rong
authors.initials: R
authors.lastname: Shi
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Song
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000328:Adult; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification; D013094:Spermatozoa / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D012313:RNA