Dioxin immunosensor using anti-2,3,7,8-TCDD antibody which was produced with mono 6-(2,3,6,7-tetrachloroxanthene-9-ylidene) hexyl succinate as a hapten.
To detect dioxin using a quartz crystal microbalance (QCM) immunosensor, anti-2,3,7,8-tetrachloro-p-dibenzodioxin (TCDD) monoclonal antibodies (MAbs) were produced as types of IgG1 and IgM, with mono 6-(2,3,6,7-tetrachloroxanthene-9-ylidene) hexyl succinate (as a hapten) conjugated with bovine serum albumin (dioxin-BSA). Furthermore, ScFv was generated from hybridoma-producing IgG1 MAb. Among these antibodies, ScFv showed excellent capability for dioxin detection using QCM immunosensors.
Park
Jong-Won
JW
Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), 1-1 Higashi, Tsukuba 305-8565, Japan.
Kurosawa
Shigeru
S
Aizawa
Hidenobu
H
Hamano
Hirokazu
H
Harada
Yoshitsugu
Y
Asano
Satoko
S
Mizushima
Yutaka
Y
Higaki
Megumu
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004147:Dioxins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D000072317:Polychlorinated Dibenzodioxins / Q000737:chemistry / Q000276:immunology; D013386:Succinates / Q000737:chemistry* / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000032:analysis
D000911:Antibodies, Monoclonal; D004147:Dioxins; D006241:Haptens; D000072317:Polychlorinated Dibenzodioxins; D013386:Succinates; D014874:Water Pollutants, Chemical