Influence of fluid resuscitation on renal microvascular PO2 in a normotensive rat model of endotoxemia.
BACKGROUND
Septic renal failure is often seen in the intensive care unit but its pathogenesis is only partly understood. This study, performed in a normotensive rat model of endotoxemia, tests the hypotheses that endotoxemia impairs renal microvascular PO2 (microPO2) and oxygen consumption (VO2,ren), that endotoxemia is associated with a diminished kidney function, that fluid resuscitation can restore microPO2, VO2,ren and kidney function, and that colloids are more effective than crystalloids.


METHODS
Male Wistar rats received a one-hour intravenous infusion of lipopolysaccharide, followed by resuscitation with HES130/0.4 (Voluven), HES200/0.5 (HES-STERIL 6%) or Ringer's lactate. The renal microPO2 in the cortex and medulla and the renal venous PO2 were measured by a recently published phosphorescence lifetime technique.


RESULTS
Endotoxemia induced a reduction in renal blood flow and anuria, while the renal microPO2 and VO2,ren remained relatively unchanged. Resuscitation restored renal blood flow, renal oxygen delivery and kidney function to baseline values, and was associated with oxygen redistribution showing different patterns for the different compounds used. HES200/0.5 and Ringer's lactate increased the VO2,ren, in contrast to HES130/0.4.


CONCLUSIONS
The loss of kidney function during endotoxemia could not be explained by an oxygen deficiency. Renal oxygen redistribution could for the first time be demonstrated during fluid resuscitation. HES130/0.4 had no influence on the VO2,ren and restored renal function with the least increase in the amount of renal work.
Johannes
Tanja
T
Department of Physiology, Academic Medical Center, University of Amsterdam, The Netherlands. t.johannes@amc.uva.nl
Mik
Egbert G
EG
Nohé
Boris
B
Raat
Nicolaas J H
NJ
Unertl
Klaus E
KE
Ince
Can
C
D000818:Animals; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000503:physiopathology / Q000628:therapy*; D005440:Fluid Therapy / Q000379:methods*; D007668:Kidney / Q000098:blood supply* / Q000502:physiology*; D008297:Male; D008833:Microcirculation / Q000502:physiology; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar