[Effects of microRNA miR-181a on gene expression profiles of K562 cells].
OBJECTIVE
To investigate the effect of microRNA on the gene expression profile of human leukemia K562 cells using microarray technique.


METHODS
miR-181a RNA duplexes were designed and synthesized according to the mature sequence of miR-181a. Forty-eight hours after transfection of in vitro cultured K562 cells using Oligofectamine, gene expression profiles of the cells were studied and analyzed using Agilent Human 1A Oligo microarray.


RESULTS
Totalling 228 differentially expressed genes were identified from the 20,173 screened genes, including 59 up-regulated ones (consisting of metabolism-associated genes, tumor suppressor genes, signal transduction-associated genes, immunity and defense-associated genes etc), and 169 down-regulated ones (consisting of oncogenes, DNA-binding and transcription genes, metabolism-associated genes, signal transduction-associated genes, cell cycle and development-associated genes etc.) in the transfected K562 cells as compared with the control K562 cells. Changes in expressions of CTCF, ZAP70, SEMA4C and RALA were confirmed by semi-quantitative reverse transcription-polymerase chain reaction.


CONCLUSIONS
miR-181a transfection for 48 h induces gene expression profile changes in K562 cells, indicating the functionality of the miR-181a. These differentially expressed genes are related to the functions of the microRNA, and may also be the basis of the regulation model of posttranscriptional gene silencing. These findings provide an evidence for further study of the machineries and functions of the microRNA in mammalian cells.
Zhou
Jue-yu
JY
Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China. zhoujueyu@yahoo.com.cn
Ma
Wen-li
WL
Fei
Jia
J
Ding
Da-peng
DP
Shi
Rong
R
Jiang
Li
L
Zheng
Wen-ling
WL
D020869:Gene Expression Profiling; D006801:Humans; D020014:K562 Cells; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D035683:MicroRNAs; D034741:RNA, Small Interfering