[Effect of Slit-Robo signal on apoptosis of oral cancer cell line Tb].
OBJECTIVE
To study the effect of Slit-Robo signal on apoptosis of human oral squamous cell carcinoma line Tb.


METHODS
After the treatment in Tb cells with monoclonal antibodies (mAb) R5 of against Robo1 receptor extracellular domain, the apoptosis of Tb cell was examined by clone formation assay, flow cytometry, DNA ladder and Hochst-PI. The expression of fas and fasL proteins was observed by Western blotting analysis.


RESULTS
After the treatment by R5 mAb, the proliferative rate decreased and the apoptotic rates increased, and the expression of fas and fasL proteins was up-regulated.


CONCLUSIONS
Slit-Robo signal could inhibit the apoptosis of tumor cells during genesis of tongue cancer by regulating the expression of fas-fasL proteins.
Ma
Yu-guang
YG
Department of Breast Diseases, The First Hospital of Lanzhou University, Lanzhou 730000, China.
Wang
Li-jing
LJ
Han
Bing
B
Zhang
Jie
J
D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D011971:Receptors, Immunologic; D015398:Signal Transduction; D019014:fas Receptor / Q000378:metabolism; D000097905:Roundabout Proteins
D053222:Fas Ligand Protein; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D019014:fas Receptor; D000097905:Roundabout Proteins