Protein enrichment of potato processing waste through yeast fermentation.
Potato starch obtained from waste waters of chips manufacturing was used as a fermentation substrate for yeast protein enrichment. Among 18 yeast strains, 6 strains were screened according to their biomass yield and protein content after fermentation for 16 h at 30 degrees C in an aerated glucose-based liquid media (4.5 Ls). Using concentrated media (25% solids) made from potato starch pre-hydrolyzed with malt flour and batch-fermented for 20 h at 26 degrees C under aerobic conditions, Candida utilis ATCC 9256 was the most efficient protein-forming strain. Scaled-up at the 100 Ls level, the aerobic batch process was improved under fed-batch conditions with molasses supplementation. After drying, fermented starch contained 11-12% protein, including 7-8% yeast protein.
Gélinas
P
P
Food Research and Development Centre, 3600, Casavant Blvd. West, Saint-Hyacinthe, Que., Canada J2S 8E3. gelinasp@agr.gc.ca
Barrette
J
J
D002175:Candida / Q000378:metabolism; D003454:Cryptococcus / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005525:Food-Processing Industry; D005656:Fungal Proteins / Q000378:metabolism*; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D007218:Industrial Microbiology; D007716:Kluyveromyces / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D011198:Solanum tuberosum / Q000378:metabolism*; D013213:Starch / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism*
D003470:Culture Media; D005656:Fungal Proteins; D013213:Starch; D005947:Glucose