The efficacy of certain anti-tuberculosis drugs is affected by binding to alpha-1-acid glycoprotein.
One of the most ubiquitous plasma proteins, alpha-1-acid glycoprotein (AGP), has a high affinity, low capacity binding for basic drugs positively charged at physiological pH. Moreover, as an acute phase protein its level is increased in various disease states in a manner that is likely to influence the free plasma level of a drug, the ability to attain minimum effective concentration and overall in vivo effectiveness. AGP is a glycoprotein known to display disease specific changes in glycosylation and although this secondary modification is not directly involved in drug binding, it may influence the conformation of the binding site. Binding studies reveal that alpha-1-acid glycoprotein bind mainly to the tuberculosis drugs: rifampicin; isoniazid; pyrazinamide; p-aminosalicylic acid; capreomycin; ethionamide; levofloxacin and ofloxacin out with the therapeutic plasma range tested. These results are however still considered significant as not only are alpha-1-acid glycoprotein levels increased during the acute phase response but specific alpha-1-acid glycoprotein from tuberculosis samples are subject to glycosylation changes which can increase the binding affinity and cause binding to occur at the therapeutic concentration.
Johnson
Deborah-Ann
DA
Department of Bioscience, Royal College, Glasgow, UK.
Smith
Kevin D
KD
D000995:Antitubercular Agents / Q000378:metabolism* / Q000627:therapeutic use*; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009961:Orosomucoid / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D000995:Antitubercular Agents; D009961:Orosomucoid