An alternative approach to amyloid fibrils morphology: CdSe/ZnS quantum dots labelled beta-amyloid peptide fragments Abeta (31-35), Abeta (1-40) and Abeta (1-42).
Abeta (31-35) peptide and control peptides as well as full length Abeta (1-40) and Abeta (1-42) peptides were labelled with luminescent CdSe/ZnS quantum dots (QDs) to observe the morphology of amyloid fibers. A comparison was made between QDs and an organic dye, namely Dansyl group, which showed that the QDs present a much better contrast for imaging than the organic dye.
Ji
Xiaojun
X
Department of Chemistry, University of Miami, 1301 Memorial Drive, Room 315, Coral Gables, FL 33124, USA. x.ji@umiami.edu
Naistat
David
D
Li
Changqing
C
Orbulesco
Jhony
J
Leblanc
Roger M
RM
D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000648:ultrastructure*; D002942:Circular Dichroism; D004396:Coloring Agents; D003619:Dansyl Compounds; D006801:Humans; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D010446:Peptide Fragments / Q000737:chemistry* / Q000648:ultrastructure*; D045663:Quantum Dots
D016229:Amyloid beta-Peptides; D004396:Coloring Agents; D003619:Dansyl Compounds; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); C113042:amyloid beta-protein (31-35); C033853:dansyl acid