Cloning and expression analysis of Mx cDNA from Senegalese sole (Solea senegalensis).
Senegalese sole (Solea senegalensis) is a promising fish species of growing interest in European aquaculture. In fish farming, viral infections are a constant threat therefore, understanding fish defence mechanisms is a main priority to avoid economic losses. Mx proteins are involved in the innate antiviral response of fish. They are induced by type I interferons (alpha and beta) and are essential to investigate viral defence mechanisms in fish, due to the difficulty in tracking interferon activity in these species. In this study a full-length Senegalese sole Mx cDNA has been RT-PCR cloned, resulting in 2322bp coding for 623 amino acids. The sequence accounts for the main characteristics of Mx proteins but lacking nuclear localisation signal (NLS), which suggests cytoplasmic localisation. The alignments of Senegalese sole Mx sequence showed the highest identity with the flatfish species, 80.1% identity with flounder and 78.9% with halibut. The spatial and temporal expression pattern has been analysed in control and challenged fish by RT-PCR. In control fish a constitutive level of sole Mx expression has been obtained and a clear induction was observed after treatment with Poly[I:C], which supports a putative role for the Mx in Senegalese sole viral defence. These findings contribute to increasing the knowledge of the role of interferon pathway in fish innate immunity and to develop new tools to fight virus infections in the culture of this species.
Fernández-Trujillo
M Alejandra
MA
Department of Genetics, Faculty of Sciences, University of Málaga, Campus de Teatinos, 29071 Málaga, Spain.
Porta
Javier
J
Borrego
Juan J
JJ
Alonso
M Carmen
MC
Alvarez
M Carmen
MC
Béjar
Julia
J
D000199:Actins / Q000032:analysis / Q000096:biosynthesis; D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology; D005412:Flatfishes / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression / Q000276:immunology*; D020869:Gene Expression Profiling / Q000662:veterinary; D006801:Humans; D008969:Molecular Sequence Data; D064547:Myxovirus Resistance Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013997:Time Factors
D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary; D064547:Myxovirus Resistance Proteins; D019204:GTP-Binding Proteins