[Estrogenic and androgenic effects of low dose raw water organics].
OBJECTIVE
To study the estrogenic and (anti)androgenic effects caused by low-dose raw water organic compounds.


METHODS
The organic compounds from raw water was concentrated with solid-phase extraction. Rodent 3-day Uterotrophic Assay and Hershberger assay, the assays recommended in EPA Tier 1 Screening Program for EDCs, were employed to investigate estrogenic and (anti) androgenic effects of these chemical mixture exposure. Doses equivalent to human daily intake were administered to laboratory animals.


RESULTS
Low-dose effects of water organic compounds,which refer to levels of human daily water intake, did not show a statistical difference of weights of mouse uterus and rat sexual accessory glands(SAG) from the negative control in Uterotrophic Assay and Hershberger Assay, except the height of uterus epithelial in 4.0 L / 60 kg was significant difference with negative control.


CONCLUSIONS
Based upon the water sample tested, it did not suggest that the low-dose raw water organic compounds exposure equivalent to people daily drinking water intake cause rodents (anti)androgenic in vivo effects, but it demonstrated a suspected estrogenic effect.
Zhang
Guo-zhou
GZ
Key Laboratory of the Public Health and Safety Ministry of Education Department of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, China.
Zhang
Yu-jie
YJ
Wang
Xia
X
Yin
Li-hong
LH
D000728:Androgens / Q000633:toxicity*; D000818:Animals; D002681:China; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D005618:Fresh Water / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D009930:Organic Chemicals / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply / Q000032:analysis*
D000728:Androgens; D004968:Estrogens, Non-Steroidal; D009930:Organic Chemicals; D014874:Water Pollutants, Chemical