[The effect of oncolytic adenovirus on human umbilical vein endothelial cell].
The E1A gene was obtained by PCR with QBI-293A cell genome DNA as template. After enzyme digestion, the E1A gene was ligated to transfer vector pAdTrack-CMV. The positive clone pAdTrack-CMV-E1A were lineared by PmeI and co-transformed with pAdEasy-1 in BJ5183 E. coli. The recombinant adenovirus vector pAdEasy-1-pAdTrack-CMV-E1A were digested by PacI and transfected into QBI-293A cells with liposomes. The oncolytic recombinant adenovirus Ad-E1A was obtained after 7 days. The results showed that this oncolytic adenovirus Ad-E1A can replicate in ECV304 cells and inhibit growth of ECV304 cell. In addition, it also decreased the secretion of VEGF and expression of NF-kappaB of ECV304 cells, indicating that Ad-E1A have potential of inhibition of tumor metastasis.
Ye
Zhen-Min
ZM
Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China.
Wang
Xiao-Hua
XH
Zhong
Jiang
J
Miao
Jing-Cheng
JC
Sheng
Wei-Hua
WH
Xie
Yu-Feng
YF
Wang
Jin-Zhi
JZ
Yang
Ji-Cheng
JC
D000256:Adenoviridae / Q000235:genetics / Q000502:physiology*; D017500:Adenovirus E1A Proteins / Q000096:biosynthesis* / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D050130:Oncolytic Virotherapy; D050504:Oncolytic Viruses / Q000235:genetics / Q000502:physiology*; D011401:Promoter Regions, Genetic; D014471:Umbilical Veins / Q000166:cytology* / Q000378:metabolism
D017500:Adenovirus E1A Proteins