Changes in antioxidant defense enzymes after d-amphetamine exposure: implications as an animal model of mania.
Studies have demonstrated that oxidative stress is associated with amphetamine-induced neurotoxicity, but little is known about the adaptations of antioxidant enzymes in the brain after amphetamine exposure. We studied the effects of acute and chronic amphetamine administration on superoxide dismutase (SOD) and catalase (CAT) activity, in a rodent model of mania. Male Wistar rats received either a single IP injection of D-: amphetamine (1 mg/kg, 2 mg/kg, or 4 mg/kg) or vehicle (acute treatment). In the chronic treatment rats received a daily IP injection of either D-: amphetamine (1 mg/kg, 2 mg/kg, or 4 mg/kg) or vehicle for 7 days. Locomotor behavior was assessed using the open field test. SOD and CAT activities were measured in the prefrontal cortex, hippocampus, and striatum. Acute and to a greater extent chronic amphetamine treatment increased locomotor behavior and affected SOD and CAT activities in the prefrontal cortex, hippocampus and striatum. Our findings suggest that amphetamine exposure is associated with an imbalance between SOD and CAT activity in the prefrontal cortex, hippocampus and striatum.
Frey
Benício N
BN
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, 90035-003 Porto Alegre, RS, Brazil.
Valvassori
Samira S
SS
Réus
Gislaine Z
GZ
Martins
Márcio R
MR
Petronilho
Fabrícia C
FC
Bardini
Katrine
K
Dal-Pizzol
Felipe
F
Kapczinski
Flávio
F
Quevedo
João
J
D000661:Amphetamine / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001714:Bipolar Disorder / Q000378:metabolism*; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D002374:Catalase / Q000378:metabolism*; D015259:Dopamine Agents / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
D000975:Antioxidants; D015259:Dopamine Agents; D000661:Amphetamine; D002374:Catalase; D013482:Superoxide Dismutase