title: In situ hybridization of whole-mount embryos.
abstract: The ability to visualize the expression of a gene in both time and space is an essential tool of developmental biology. Here, we detail a robust method for in situ hybridization of RNA probes to whole pieces of fixed tissue. This method has been optimized for reliable and sensitive visualization of the spatial patterns of gene expression in mouse embryo tissue.
authors.affiliation: Institute of Molecular Bioscience, The University of Queensland, Brisbane, Australia.
authors.forename: Murray
authors.initials: M
authors.lastname: Hargrave
authors.forename: Josephine
authors.initials: J
authors.lastname: Bowles
authors.forename: Peter
authors.initials: P
authors.lastname: Koopman
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D009693:Nucleic Acid Hybridization / Q000379:methods; D011247:Pregnancy; D015347:RNA Probes / Q000138:chemical synthesis; D014158:Transcription, Genetic
chemical_list: D015347:RNA Probes