title: An annotated cDNA library of juvenile Euprymna scolopes with and without colonization by the symbiont Vibrio fischeri.
abstract: BACKGROUND
  Biologists are becoming increasingly aware that the interaction of animals, including humans, with their coevolved bacterial partners is essential for health. This growing awareness has been a driving force for the development of models for the study of beneficial animal-bacterial interactions. In the squid-vibrio model, symbiotic Vibrio fischeri induce dramatic developmental changes in the light organ of host Euprymna scolopes over the first hours to days of their partnership. We report here the creation of a juvenile light-organ specific EST database.
  
  
  RESULTS
  We generated eleven cDNA libraries from the light organ of E. scolopes at developmentally significant time points with and without colonization by V. fischeri. Single pass 3' sequencing efforts generated 42,564 expressed sequence tags (ESTs) of which 35,421 passed our quality criteria and were then clustered via the UIcluster program into 13,962 nonredundant sequences. The cDNA clones representing these nonredundant sequences were sequenced from the 5' end of the vector and 58% of these resulting sequences overlapped significantly with the associated 3' sequence to generate 8,067 contigs with an average sequence length of 1,065 bp. All sequences were annotated with BLASTX (E-value < -03) and Gene Ontology (GO).
  
  
  CONCLUSIONS
  Both the number of ESTs generated from each library and GO categorizations are reflective of the activity state of the light organ during these early stages of symbiosis. Future analyses of the sequences identified in these libraries promise to provide valuable information not only about pathways involved in colonization and early development of the squid light organ, but also about pathways conserved in response to bacterial colonization across the animal kingdom.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706, USA. cchun@wisc.edu
authors.forename: Carlene K
authors.initials: CK
authors.lastname: Chun
authors.forename: Todd E
authors.initials: TE
authors.lastname: Scheetz
authors.forename: Maria de Fatima
authors.initials: Mde F
authors.lastname: Bonaldo
authors.forename: Bartley
authors.initials: B
authors.lastname: Brown
authors.forename: Anik
authors.initials: A
authors.lastname: Clemens
authors.forename: Wendy J
authors.initials: WJ
authors.lastname: Crookes-Goodson
authors.forename: Keith
authors.initials: K
authors.lastname: Crouch
authors.forename: Tad
authors.initials: T
authors.lastname: DeMartini
authors.forename: Mari
authors.initials: M
authors.lastname: Eyestone
authors.forename: Michael S
authors.initials: MS
authors.lastname: Goodson
authors.forename: Bernadette
authors.initials: B
authors.lastname: Janssens
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Kimbell
authors.forename: Tanya A
authors.initials: TA
authors.lastname: Koropatnick
authors.forename: Tamara
authors.initials: T
authors.lastname: Kucaba
authors.forename: Christina
authors.initials: C
authors.lastname: Smith
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Stewart
authors.forename: Deyan
authors.initials: D
authors.lastname: Tong
authors.forename: Joshua V
authors.initials: JV
authors.lastname: Troll
authors.forename: Sarahrose
authors.initials: S
authors.lastname: Webster
authors.forename: Jane
authors.initials: J
authors.lastname: Winhall-Rice
authors.forename: Cory
authors.initials: C
authors.lastname: Yap
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Casavant
authors.forename: Margaret J
authors.initials: MJ
authors.lastname: McFall-Ngai
authors.forename: M Bento
authors.initials: MB
authors.lastname: Soares
mesh_terms: D048908:Aliivibrio fischeri / Q000254:growth & development*; D000818:Animals; D001483:Base Sequence; D049832:Decapodiformes / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D020224:Expressed Sequence Tags; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013559:Symbiosis / Q000502:physiology*