Stable triple-stranded DNA formation and its application to the SNP detection.
We have found that a short stretch (30mer or larger) of triple-stranded DNA structure formed at the terminus (or very near) of linear DNA molecules is unusually stable, withstanding heat treatment at as high as 95 degrees C. The stable triple-stranded structure is formed only when deoxyoligonucleotides are complementary to the strand terminating with 5'-phosphate and not to the strand terminating with 3'-OH. Presence of a single mismatched base in the complementary deoxyoligonucleotides drastically reduces the stability. We show that these unique properties of the terminal triple-stranded structure can be applied to the detection of single nucleotide polymorphisms in genomes without DNA dissociation and/or hybridization.
Shigemori
Yasushi
Y
Department of Human Gene Research, Kazusa DNA Research Institute, Kazusa-kamatari, Kisarazu, Chiba, Japan.
Oishi
Michio
M
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D020641:Polymorphism, Single Nucleotide
D004277:DNA, Single-Stranded; D004247:DNA