Wheat D-type cyclin Triae;CYCD2;1 regulate development of transgenic Arabidopsis plants.
The D-type cyclin genes play key roles in plant development of Arabidopsis. To investigate the functions of cyclins in monocots, a putative ortholog of cyclin D2 genes was isolated from wheat shoot tissues, and designated as Triae;CYCD2;1. The transcripts of Triae;CYCD2;1 were primarily localized in the proliferating tissues, particularly, in shoot apical meristem and leaf primordia of wheat plants. Ectopic expression of Triae;CYCD2;1 in Arabidopsis affected plant morphology and retarded plant growth. Further examination showed that the promotion of cell division and the inhibition of cell differentiation occurred in both transgenic plants and tissues. In vitro experiments indicated that Triae;CYCD2;1 had functional roles in responding to cytokinin and auxin. Molecular analysis revealed that the transcript levels of several cell cycle-associated genes, particularly Arath;CYCD3;1, were increased in the Arabidopsis plants with the expressing Triae;CYCD2;1. The results in this study provide new information on D-type cyclin in wheat.
Wang
Fang
F
Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China.
Huo
Sheng Nan
SN
Guo
Jing
J
Zhang
Xian Sheng
XS
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D046508:Culture Techniques; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D003583:Cytokinins / Q000378:metabolism; D015870:Gene Expression; D007210:Indoleacetic Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000166:cytology / Q000502:physiology; D018520:Plant Shoots / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000502:physiology; D014908:Triticum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015854:Up-Regulation
D029681:Arabidopsis Proteins; C486198:CycD2 protein, Arabidopsis; C496944:CycD3 protein, Arabidopsis; D016213:Cyclins; D003583:Cytokinins; D007210:Indoleacetic Acids