[Allelic polymorphism analysis of killer cell immunoglobulin-like receptor gene 3DL2 in Zhejiang Han population].
OBJECTIVE
To analyze the allelic polymorphism of killer cell immunoglobulin (Ig)-like receptor (KIR) gene 3DL2 in Zhejiang Han population.


METHODS
Allelic definition within KIR3DL2 was achieved through using the PCR-sequence specific oligonucleotide probes (PCR-SSOP) method and the specific amplification of exon 3-exon 4 and exon 8-exon 9. Twenty-one digoxigenin-labelled probes were used to the SSOP analysis.


RESULTS
Seven alleles of KIR3DL2 were observed, in which KIR3DL2 *002 had the highest allele frequency, 0.57. One sample could not be given the allele combination according to the probe profile.


CONCLUSIONS
The PCR-SSOP method was reliable. There is a distinctive allele frequency of KIR3DL2 in Zhejiang Han population.
Jiang
Kan
K
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Minstry of Health, Hangzhou, Zhejiang, 310006 PR China.
Zhu
Fa-ming
FM
Zhang
Wei
W
Lu
Qin-feng
QF
He
Ji
J
Yan
Li-xing
LX
D044466:Asian People / Q000235:genetics; D002681:China; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D054347:Receptors, KIR3DL2 / Q000235:genetics*; D017422:Sequence Analysis, DNA
D054347:Receptors, KIR3DL2