Inhibitory effects of Tabebuia impetiginosa inner bark extract on platelet aggregation and vascular smooth muscle cell proliferation through suppressions of arachidonic acid liberation and ERK1/2 MAPK activation.
The antiplatelet and antiproliferative activities of extract of Tabebuia impetiginosa inner bark (taheebo) were investigated using washed rabbit platelets and cultured rat aortic vascular smooth muscle cells (VSMCs) in vitro. n-Hexane, chloroform and ethyl acetate fractions showed marked and selective inhibition of platelet aggregation induced by collagen and arachidonic acid (AA) in a dose-dependent manner. These fractions, especially the chloroform fraction, also significantly suppressed AA liberation induced by collagen in [(3)H]AA-labeled rabbit platelets. The fractions, especially the chloroform fraction, potently inhibited cell proliferation and DNA synthesis induced by platelet derived growth factor (PDGF)-BB, and inhibited the levels of phosphorylated extracellular signal regulated kinase (ERK1/2) mitogen activated protein kinase (MAPK) stimulated by PDGF-BB, in the same concentration range that inhibits VSMC proliferation and DNA synthesis.
Son
Dong-Ju
DJ
College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Lim
Yong
Y
Park
Young-Hyun
YH
Chang
Sung-Keun
SK
Yun
Yeo-Pyo
YP
Hong
Jin-Tae
JT
Takeoka
Gary R
GR
Lee
Kwang-Geun
KG
Lee
Sung-Eun
SE
Kim
Mi-Ran
MR
Kim
Jeong-Han
JH
Park
Byeoung-Soo
BS
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects*; D024301:Plant Bark / Q000737:chemistry*; D028321:Plant Preparations / Q000737:chemistry / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D029663:Tabebuia / Q000737:chemistry*
D028321:Plant Preparations; D016718:Arachidonic Acid; D048049:Extracellular Signal-Regulated MAP Kinases