Invader assay for single-nucleotide polymorphism genotyping and gene copy number evaluation.
The Invader assay (Third Wave Technologies) is a homogeneous, isothermal DNA probe-based method for sensitive detection of nucleic acid sequences. Invader reactions are performed directly on genomic DNA or total RNA targets; however, polymerase chain reaction- or reverse transcriptase polymerase chain re action-amplified products can also be used. Detection is achieved through target-specific signal amplification instead of target amplification. The assay is a highly accurate and specific detection method for both qualitative and quantitative analysis of single-nucleotide changes, insertions or deletions, gene copy number, infectious agents, and gene expression.
Mast
Andrea
A
Third Wave Technologies Inc., Madison, WI, USA.
de Arruda
Monika
M
D004247:DNA / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes; D018628:Gene Dosage; D005838:Genotype; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D020641:Polymorphism, Single Nucleotide
D005456:Fluorescent Dyes; D015335:Molecular Probes; D004247:DNA