Scanning holographic microscopy of three-dimensional fluorescent specimens.
We demonstrate experimentally the three-dimensional reconstructions of fluorescent biological specimens using scanning holographic microscopy. Three-dimensional reconstructions with transverse resolution below about 1 microm of transmission and fluorescence emission images are presented and analyzed. The limitations of the method are discussed.
Indebetouw
Guy
G
Department of Physics, Virginia Tech, Blacksburg, Virginia 24061-0435, USA. gindebet@vt.edu
Zhong
Wenwei
W
D000465:Algorithms; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity