title: High-throughput affinity mass spectrometry.
abstract: Affinity mass spectrometry (AMS) is a proteomics approach for selectively isolating target protein(s) from complex biological fluids for mass spectrometric analysis. The resulting high-content mass spectrometry (MS) data show the unique MS protein signatures (wild-type, posttranslationally modified, as well as genetically modified forms of the protein target) that are present within a biological sample. Information regarding such protein diversity is normally lost in classical proteomic or immunoassay analyses. This chapter presents a step-by-step description of high-throughput AMS in the population proteomic screening of the human plasma protein cystatin C.
authors.affiliation: Intrinsic Bioprobes Inc., Tempe, AZ, USA.
authors.forename: Urban A
authors.initials: UA
authors.lastname: Kiernan
authors.forename: Dobrin
authors.initials: D
authors.lastname: Nedelkov
authors.forename: Eric E
authors.initials: EE
authors.lastname: Niederkofler
authors.forename: Kemmons A
authors.initials: KA
authors.lastname: Tubbs
authors.forename: Randall W
authors.initials: RW
authors.lastname: Nelson
mesh_terms: D000595:Amino Acid Sequence; D001331:Automation; D001798:Blood Proteins / Q000737:chemistry; D002138:Calibration; D055316:Cystatin C; D015891:Cystatins / Q000097:blood / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D017354:Point Mutation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D012371:Robotics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001798:Blood Proteins; C525030:CST3 protein, human; D055316:Cystatin C; D015891:Cystatins; D011506:Proteins