title: Degradation characteristics and metabolic pathway of 17alpha-ethynylestradiol by Sphingobacterium sp. JCR5.
abstract: A 17alpha-ethynylestradiol (EE2)-degrading bacterium was isolated from the activated sludge of the wastewater treatment plant (WWTP) of an oral contraceptives producing factory in Beijing, China. On the basis of its morphology, biochemical properties and the 16S rDNA sequence analysis, this strain was identified as Sphingobacterium sp. JCR5. This strain grew on EE2 as sole source of carbon and energy, and metabolized up to 87% of the substrate added (30 mgl-1) within 10 d at 30 degrees C. In addition to EE2 the strain could be cultivated on steroidal estrogens like estrone (E1), 17beta-estradiol (E2), estriol (E3) and mestranol (MeEE2), the intermediates of contraceptive medicine processing and on some aromatic compounds. Mass spectrum analysis of the EE2 degradation showed that in the first step it is oxygenized to E1, 2-hydroxy-2,4-dienevaleric acid and 2-hydroxy-2,4-diene-1,6-dioic acid, which are the main catabolic intermediates. The former was analogous to the pathway of a previously reported testosterone-degrading bacterium Comamonas testosteroni TA441 and the latter is a metabolite with a different cleavage position of 3-hydroxy-4,5-9,10-disecoestrane-1(10),2-diene-5,9,17-trione-4-oic acid from the former.
authors.affiliation: College of Environment and Energy Engineering, Beijing University of Technology, No. 100, Pingleyuan Road, Chaoyang District, Beijing 100022, China.
authors.forename: Ren
authors.initials: R
authors.lastname: Haiyan
authors.forename: Ji
authors.initials: J
authors.lastname: Shulan
authors.forename: Naeem
authors.initials: N
authors.lastname: ud din Ahmad
authors.forename: Wang
authors.initials: W
authors.lastname: Dao
authors.forename: Cui
authors.initials: C
authors.lastname: Chengwu
mesh_terms: D004997:Ethinyl Estradiol / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D015394:Molecular Structure; D045249:Sphingobacterium / Q000254:growth & development / Q000378:metabolism*
chemical_list: D004997:Ethinyl Estradiol