title: Determination of optimal conditions for studies of electrophoretic mobility of human erythrocytes loaded with cryoglobulins.
abstract: For studies of the charge characteristics of human erythrocytes loaded with cryoglobulins the cells should be stored in Alsever solution with 0.1% sodium azide no longer than 1 week after blood collection. Erythrocytes should be loaded with cryoglobulins by incubation during at least 4 h, the final concentration of cryoproteins in the solution being no lower than 30 microg/ml.
authors.affiliation: Department of Experimental and Theoretical Physics, Russian State Medical University, Moscow. cryoglobulin@mail.ru
authors.forename: N A
authors.initials: NA
authors.lastname: Konstantinova
authors.forename: E N
authors.initials: EN
authors.lastname: Karandashov
mesh_terms: D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D003450:Cryoglobulins / Q000737:chemistry* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D019810:Sodium Azide / Q000494:pharmacology; D013048:Specimen Handling; D013997:Time Factors
chemical_list: D003450:Cryoglobulins; D019810:Sodium Azide