Synthesis and in vitro cytotoxicity of haloderivatives of noscapine.
Three haloderivatives of noscapine 2-4 were synthesized chemoselectively and their in vitro cytotoxicity was assessed by MTT assay on U-87 human glioblastoma cell lines. At 50 microM concentration after 72 h, 9-chloronoscapine 2, 9-bromonoscapine 3 (EM011), and 9-iodonoscapine 4 killed 87.8%, 51.2%, and 56.8% cells, respectively, however noscapine kills only 40% of the cells; revealing 9-chloronoscapine as a potential cytotoxic agent than noscapine and 9-bromonoscapine (EM011). At low concentration (1 microM) 9-bromonoscapine (46.7%) and 9-chloronoscapine (45.7%) did not show any significant difference.
Verma
Akhilesh Kumar
AK
Synthetic Organic Chemistry Research Laboratory, Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110007, India. akhilesh682000@yahoo.com
Bansal
Sandhya
S
Singh
Jaspal
J
Tiwari
Rakesh Kumar
RK
Kasi Sankar
V
V
Tandon
Vibha
V
Chandra
Ramesh
R
D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005909:Glioblastoma / Q000188:drug therapy / Q000473:pathology; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D009665:Noscapine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; D013844:Thiazoles; D009665:Noscapine; C022616:thiazolyl blue