High-resolution NMR spectroscopy of a GPCR in aligned bicelles.
Solid-state NMR spectra with single-site resolution of CXCR1, a G protein-coupled receptor (GPCR), were obtained in magnetically aligned phospholipid bicelles. These results demonstrate that GPCRs in phospholipid bilayers are suitable samples for structure determination by solid-state NMR. The spectra also enable studies of drug-receptor interactions.
Park
Sang Ho
SH
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0307, USA.
Prytulla
Stefan
S
De Angelis
Anna A
AA
Brown
Jonathan Miles
JM
Kiefer
Hans
H
Opella
Stanley J
SJ
D001665:Binding Sites; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000378:metabolism; D023062:Receptors, Interleukin-8A / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism
D008051:Lipid Bilayers; D043562:Receptors, G-Protein-Coupled; D023062:Receptors, Interleukin-8A; D019204:GTP-Binding Proteins