Gas chromatography-mass spectrometry determination of the pentafluorobenzoyl derivative of methylhydrazine in false morel (Gyromitra esculenta) as a monitor for the content of the toxin gyromitrin.
The main toxic compound found in false morel (Gyromitra esculenta) is acetaldehyde-N-methyl-N-formylhydrazone (gyromitrin). This paper describes a method of determining the total hydrazones content based on acid hydrolysis of gyromitrin and other related hydrazones in air-dried false morel followed by derivatisation of methylhydrazine with pentafluorobenzoyl chloride. The derivative, tris-pentafluorobenzoyl methylhydrazine (tris-PFB-MH) is analyzed by gas chromatography-mass spectrometry. The overall precision of the method is better than 10% (relative standard deviation) for 0.5 ng/microl methylhydrazine in solution. The minimum detectable concentration of methylhydrazine (tris-PFB-MH) by this method is estimated to be approximately 12 pg/microl, which is equal to 0.3 microg/g dry matter (DM) of false morel.
Arshadi
Mehrdad
M
Biomass Technology and Chemistry, Swedish University of Agricultural Sciences, PO Box 4097, SE-904 03 Umeå, Sweden. mehrdad.arshadi@btk.slu.se
Nilsson
Calle
C
Magnusson
Bengt
B
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D001203:Ascomycota / Q000737:chemistry*; D001565:Benzoates / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D009002:Monomethylhydrazine / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry; D005464:Fluorobenzenes; D005466:Fluorocarbons
D000079:Acetaldehyde; D001565:Benzoates; D009002:Monomethylhydrazine; D009183:Mycotoxins; C014737:pentafluorobenzoyl chloride; D005464:Fluorobenzenes; D005466:Fluorocarbons; C005666:gyromitrin