title: The effect of silica nanoparticulate coatings on serum protein adsorption and cellular response.
abstract: Serum protein adsorption on colloidal silica surfaces was investigated using a quartz crystal microbalance with dissipation (QCM-D) monitoring. The amount of serum proteins adsorbed on colloidal silica-coated surfaces was not significantly different from the control silica surfaces, with the exception of 21nm colloidal silica which experienced significantly less (P<0.05) fibrinogen adsorption compared with control silica. The adhesion and proliferation of human endothelial cells (C11STH) on nano-scale colloidal silica surfaces were significantly reduced compared with control silica surfaces, suggesting that the conformation of adsorbed proteins on the colloidal silica surfaces plays a role in modulating the amount of cell binding. Fibronectin is one of the main extracellular matrix proteins involved in endothelial cell attachment to biomaterial surfaces. There was reduced binding of a monoclonal anti-fibronectin antibody, that reacted specifically with the cell-binding fragment, to fibronectin-coated colloidal silica surfaces compared with control silica surfaces. This suggests that the fibronectin adsorbed on the colloidal silica-coated surfaces was conformationally changed compared with control silica reducing the availability of the cell-binding domain of fibronectin.
authors.affiliation: Graduate School of Biomedical Engineering, The University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: M S
authors.initials: MS
authors.lastname: Lord
authors.forename: B G
authors.initials: BG
authors.lastname: Cousins
authors.forename: P J
authors.initials: PJ
authors.lastname: Doherty
authors.forename: J M
authors.initials: JM
authors.lastname: Whitelock
authors.forename: A
authors.initials: A
authors.lastname: Simmons
authors.forename: R L
authors.initials: RL
authors.lastname: Williams
authors.forename: B K
authors.initials: BK
authors.lastname: Milthorpe
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005353:Fibronectins / Q000737:chemistry; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D001798:Blood Proteins; D005353:Fibronectins; D012822:Silicon Dioxide