title: A novel method for construction of gene fragment library to searching epitopes.
abstract: Identification of the epitope sequence or the functional domain of proteins is a laborious process but a necessary one for biochemical and immunological research. To achieve intensive and effective screening of these functional peptides in various molecules, we established a novel screening method using a phage library system that displays various lengths and parts of peptides derived from target protein. Applying this library for epitope mapping, epitope peptide was more efficiently identified from gene fragment library than conventional random peptide library. Our system may be a most powerful method for identifying functional peptides.
authors.affiliation: Laboratory of Pharmaceutical Proteomics, National Institute of Biomedical Innovation, 7-6-8 Asagi, Saito, Ibaraki, Osaka 567-0085, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kawamura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Shibata
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Kamada
authors.forename: Takayuki
authors.initials: T
authors.lastname: Okamoto
authors.forename: Yohei
authors.initials: Y
authors.lastname: Mukai
authors.forename: Toshiki
authors.initials: T
authors.lastname: Sugita
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Abe
authors.forename: Sunao
authors.initials: S
authors.lastname: Imai
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nomura
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nagano
authors.forename: Tadanori
authors.initials: T
authors.lastname: Mayumi
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tsutsumi
authors.forename: Shin-ich
authors.initials: SI
authors.lastname: Tsunoda
mesh_terms: D000595:Amino Acid Sequence; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D018604:Epitope Mapping / Q000379:methods*; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D014409:Tumor Necrosis Factor-alpha