title: Amino acid sequence of the Amur tiger prion protein.
abstract: Prion diseases are fatal neurodegenerative disorders in human and animal associated with conformational conversion of a cellular prion protein (PrP(C)) into the pathologic isoform (PrP(Sc)). Various data indicate that the polymorphisms within the open reading frame (ORF) of PrP are associated with the susceptibility and control the species barrier in prion diseases. In the present study, partial Prnp from 25 Amur tigers (tPrnp) were cloned and screened for polymorphisms. Four single nucleotide polymorphisms (T423C, A501G, C511A, A610G) were found; the C511A and A610G nucleotide substitutions resulted in the amino acid changes Lysine171Glutamine and Alanine204Threoine, respectively. The tPrnp amino acid sequence is similar to house cat (Felis catus ) and sheep, but differs significantly from other two cat Prnp sequences that were previously deposited in GenBank.
authors.affiliation: National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.
authors.forename: Changde
authors.initials: C
authors.lastname: Wu
authors.forename: Wanyong
authors.initials: W
authors.lastname: Pang
authors.forename: Deming
authors.initials: D
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000836:Animals, Zoo / Q000378:metabolism*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011328:Prions / Q000235:genetics*; D016415:Sequence Alignment; D013045:Species Specificity; D046010:Tigers / Q000378:metabolism*
chemical_list: D011328:Prions