title: Diagnosis of mixed Plasmodium malariae and P. vivax infection in a development aid volunteer by examination of bone-marrow specimens by real-time PCR.
abstract: Mixed Plasmodium malariae and P. vivax infections in humans are reported very infrequently. The case of a 27-year-old male who sustained malaria quartana/tertiana caused by an unbalanced mixed P. malariae-P. vivax infection is reported here. Conventional tests and serology for malarial parasites were uniformly negative. Identification and quantification of the parasites were accomplished by examining bone-marrow specimens using specific real-time TaqMan PCR.
authors.affiliation: Center for Tropical Diseases and Travel Medicine, Hospital of the Justus-Liebig University Giessen, Institute of Medical Microbiology, Frankfurter Strasse 107, Giessen, Germany.
authors.forename: Can
authors.initials: C
authors.lastname: Imirzalioglu
authors.forename: Nedim
authors.initials: N
authors.lastname: Soydan
authors.forename: Markus
authors.initials: M
authors.lastname: Schaller
authors.forename: Reinhard G
authors.initials: RG
authors.lastname: Bretzel
authors.forename: Trinad
authors.initials: T
authors.lastname: Chakraborty
authors.forename: Eugen
authors.initials: E
authors.lastname: Domann
mesh_terms: D000328:Adult; D000818:Animals; D001853:Bone Marrow / Q000469:parasitology*; D003906:Developing Countries; D006801:Humans; D008288:Malaria / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology; D016780:Malaria, Vivax / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D010965:Plasmodium malariae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010966:Plasmodium vivax / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013397:Sudan; D019914:Taq Polymerase; D014838:Volunteers
chemical_list: D019914:Taq Polymerase