title: [Simultaneous determination of three kinds of components in sanhuang tablets by high-performance liquid chromatography].
abstract: OBJECTIVE
  To establish an assay method for the determination of three kinds of biologically active components, five compounds (emodin, chrysophanol, baicalin, wogonin and berberine hydrochloride) simultaneously in Sanhuang tablets.
  
  
  METHODS
  HPLC was carried out, using a C18 column (150 mm x4. 6 mm ID, 5 microm) set at 30 degrees C, acetonitrile-0.02 mol x L(-1) acetic ammonium (adjusted pH to 3. 50 with acetic acid glacial) as mobile phase (using gradient) with flowing rate 1. 00 mL x min(-1) and detected at 270 nm.
  
  
  RESULTS
  The calibration curve of emodin was linear from 0.020 7 microg to 0.207 microg with r = 0.999 9, the average recovery was 99.65% with RSD 1.25%. The calibration curve of chrysophanol was linear from 0.052 microg to 0.52 microg with r = 0.999 9, and the average recovery was 100.36% with RSD 0.96%. The calibration curve of baicalin was linear from 0.250 5 microg to 2.505 microg with r =0.999 8, and the average recovery was 100.22% with RSD 1.29%. The calibration curve of wogonin was linear from 0.047 6 microg to 0.476 microg with r = 0.999 9, and the average recovery was 98.97% with RSD 1.20%. The calibration curve of berberine hydrochloride was linear from 0.053 12 microg to 0.531 2 microg with r = 0.999 5, and the average recovery was 96.02% with RSD 2.02%. The established method had also been used in the determination of the 5 compounds in 10 different batches of Sanhuang tablets.
  
  
  CONCLUSIONS
  This method was proved to be accurate and quick, and can be used for the quality control of the preparation all-around.
authors.affiliation: China Pharmaceutical University, Nanjing.
authors.forename: You-long
authors.initials: YL
authors.lastname: Feng
authors.forename: Bo-yang
authors.initials: BY
authors.lastname: Yu
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D000880:Anthraquinones / Q000032:analysis; D001599:Berberine / Q000031:analogs & derivatives / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D039323:Coptis / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D004642:Emodin / Q000032:analysis*; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012250:Rheum / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry; D013607:Tablets
chemical_list: D000880:Anthraquinones; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D013607:Tablets; D001599:Berberine; C038044:baicalin; C039639:dihydroberberine; D004642:Emodin; C027113:chrysophanic acid; C085514:wogonin