Measurement of a genotoxic hydrazine, agaritine, and its derivatives by HPLC with fluorescence derivatization in the agaricus mushroom and its products.
Agaricus blazei MURRILL mushroom products are sold as so-called health foods in Japan. However, a part of Agaricus is known to contain hydrazines. A sensitive and specific method for analyzing a genotoxic hydrazine, agaritine, and its derivatives was developed to assess the safety of Agaricus products. beta-N-(gamma-L(+)-Glutamyl)-4-(hydroxymethyl)phenylhydrazine (agaritine, AGT), 4-hydrazinylbenzylalcohol (HMPH), 4-hydrazinylbenzoic acid (CPH), 4-methylphenylhydrazine (MPH) and phenylhydrazine (PH) were converted to their correspond-ing fluorescent derivatives with 3,4-dihydro-6,7 dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride (DMEQ-COCl) as the fluorescence derivatization reagent. The detection limits (S/N=3) for CPH, AGT, PH and MPH were 422, 45.3, 16.5 and 138 fmol, respectively, in a 20 microl injection volume. Recoveries, achieved by adding known AGT amounts to the Agaricus sample and Agaricus products, ranged from 92.8 to 102%. By using this method which does not require partial purification of the Agaricus sample, the amounts of AGT in several types of foods were found to be 112-1836 microg/g dry weight.
Nagaokaa
Megumi Hamano
MH
National Institute of Health Sciences, Tokyo 158-8501, Japan. nagaoka@nihs.go.jp
Nagaoka
Hiroaki
H
Kondo
Kazunari
K
Akiyama
Hiroshi
H
Maitani
Tamio
T
D000363:Agaricales / Q000737:chemistry*; D000364:Agaricus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005453:Fluorescence; D006834:Hydrazines / Q000633:toxicity*; D009152:Mutagenicity Tests; D010659:Phenylhydrazines / Q000633:toxicity*
D006834:Hydrazines; D010659:Phenylhydrazines; C020628:4-methylphenylhydrazine; C015654:agaritine