title: [Effects of nonylphenol on testis tissue development and apoptosis of F1 generation male SD rats in weaning].
abstract: OBJECTIVE
  To study the mechanism of nonylphenol on testis tissue development and apoptosis of F1 generation male SD rats in weaning period.
  
  
  METHODS
  Nonylphenol was administrated via gastropipe to pregnant rats at doses of 50, 100 and 200 mg/kg, respectively, from the 7th day of preganacy to weaning. F1 generation male SD rats were sacrificed in the weaning period; their serum levels of nonylpheol were determined. The histopathological examination and immunohistochemical analysis(Bax,Bcl-2,Caspase-3) on testis were also carried out.
  
  
  RESULTS
  Compared with control, a higher serum nonylpheol level was found in F1 generation rats treated with nonylpheol 100 and 200 mg/kg. Histopathological examination revealed that rats treated with 200 mg/kg nonylphenol had much smaller diameter of testis seminiferous tubules when compared with those in the control group. Immunohistological analysis showed that the expression of Caspase-3 and Bax in the testis seminiferous tubules increased while the expression of Bcl-2 decreased in three experiment groups.
  
  
  CONCLUSIONS
  All the above data proved that nonylphenol, after being given to pregnant rats, caused apparent damage to the development of testis of F1 generation male SD rats in weaning period.
authors.affiliation: Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Long
authors.forename: Li
authors.initials: L
authors.lastname: Zhan
authors.forename: De-sheng
authors.initials: DS
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000096:biosynthesis; D005260:Female; D008297:Male; D009929:Organ Size; D010636:Phenols / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000166:cytology / Q000254:growth & development*; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
chemical_list: D010636:Phenols; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C025256:nonylphenol; D053148:Caspase 3