title: Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two- dimensional scanning mirror.
abstract: Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm x 0.75 mm in size and driven in two dimensions by microfabricated vertical comb electrostatic actuators can provide optical deflection angles through a range of approximately16 degrees . Using such scanners we demonstrated two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.52 kHz.
authors.affiliation: James H. Clark Center for Biomedical Engineering & Sciences, Stanford University, Stanford, California 94305, USA.
authors.forename: Wibool
authors.initials: W
authors.lastname: Piyawattanametha
authors.forename: Robert P J
authors.initials: RP
authors.lastname: Barretto
authors.forename: Tony H
authors.initials: TH
authors.lastname: Ko
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Flusberg
authors.forename: Eric D
authors.initials: ED
authors.lastname: Cocker
authors.forename: Hyejun
authors.initials: H
authors.lastname: Ra
authors.forename: Daesung
authors.initials: D
authors.lastname: Lee
authors.forename: Olav
authors.initials: O
authors.lastname: Solgaard
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Schnitzer
mesh_terms: D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007909:Lenses; D019563:Mechanics; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity