hKv1.5 channels play a pivotal role in the functions of human alveolar macrophages.
We examined the pharmacological properties, the molecular identity, and the functional roles of hKv1.5 channel in human alveolar macrophage. Some of outward K(+) current was inhibited by 4-aminopyridine and antisense oligodeoxynucleotides against hKv1.5 mRNA. Consistently, the protein and mRNA expressions of hKv1.5 channel were detected. Furthermore, the phagocytosis and migration of human alveolar macrophages were significantly suppressed when the protein expression of hKv1.5 channel was lowered by the antisense hKv1.5 oligodeoxynucleotides. These results suggest that hKv1.5 channel is expressed in human alveolar macrophages and it plays a role in phagocytosis and migration of the human alveolar macrophage.
Park
Seon-Ah
SA
Department of Pharmacology, Chonbuk National University Medical School, Jeonju, Jeonbuk, Republic of Korea.
Lee
Yong-Chul
YC
Ma
Tian-Ze
TZ
Park
Jeong-Ah
JA
Han
Myung-Kwan
MK
Lee
Hwang-Ho
HH
Kim
Hwan-Gyu
HG
Kwak
Yong-Geun
YG
D015761:4-Aminopyridine / Q000494:pharmacology; D002465:Cell Movement; D002478:Cells, Cultured; D006801:Humans; D015640:Ion Channel Gating; D051666:Kv1.5 Potassium Channel / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000502:physiology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D010587:Phagocytosis; D012333:RNA, Messenger / Q000378:metabolism; D017452:Receptors, IgG / Q000502:physiology
D051666:Kv1.5 Potassium Channel; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D017452:Receptors, IgG; D015761:4-Aminopyridine