[Preparation of polyclonal antibody against microcystin-LR].
Good quality polyclonal antibody against Microcystin-LR (MC-LR) was obtained from the New Zealand rabbit immunized with the homemade complete antigen BSA-MC-LR. Indirect ELISA shows the titer of this antibody is more than 1.5 x 10(5); Indirect competitive ELISA immobilized the coating antigen OVA-MC-LR was adapted to detect MCs, the calibration curve shows that the detection lower limit is 10 ng/L, and the range of quantitative detection was between 30 ng/L to 3 microg/L, with a good linearity, which will well potentially suit for sensitive analysis for MC-LR in drinking as well as surface water samples.
Sheng
Jian-wu
JW
ESPC State Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China.
He
Miao
M
Shi
Han-chang
HC
Qian
Yi
Y
D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis / Q000276:immunology*; D014873:Water Pollutants / Q000032:analysis*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008387:Marine Toxins; D052998:Microcystins; D014873:Water Pollutants; C057862:cyanoginosin LR