Intracellular compartmentation of cardiac fibres from rainbow trout and Atlantic cod--a general design of heart cells.
In mammalian cardiomyocytes, mitochondria and adjacent ATPases with participation of creatine kinase (CK) constitute functional compartments with an exchange of ADP and ATP delimited from cytosolic bulk solution. The question arises if this extends to ectothermic vertebrates: their low body temperature and thinner cardiomyocytes with a lower density of membrane structures may reduce the need and structural basis for compartmentation. In saponin-skinned cardiac fibres from rainbow trout and Atlantic cod, we investigated mitochondrial respiration induced by endogenous ADP generated by ATPases and its competition for this ADP with pyruvate kinase (PK) in excess. At low Ca(2+) activity (pCa = 7.0), PK lowered ATP-induced respiration by 40% in trout and 26% in cod. At high Ca(2+) activity (pCa = 5.41), PK had no effect. Additionally, ADP release from the fibres was almost zero but increased drastically upon inhibition of respiration with 1 mM Na-azide. This suggests that fibres are compartmented. PK abolished creatine-stimulated respiration in trout suggesting a less tight coupling of CK to respiration than in mammals. In conclusion, intracellular compartmentation seems to be a general feature of vertebrate cardiomyocytes, whereas the role of CK is unclear, but it seems to be less important for energy transport in species with lower metabolism.
Birkedal
Rikke
R
Zoophysiology, Department of Biological Sciences, University of Aarhus, Denmark. rbirkedal@gmail.com
Gesser
Hans
H
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000263:Adenylate Kinase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002451:Cell Compartmentation / Q000502:physiology*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D052281:Creatine Kinase, Mitochondrial Form / Q000378:metabolism; D004789:Enzyme Activation; D048389:Gadus morhua / Q000033:anatomy & histology*; D008929:Mitochondria, Heart / Q000187:drug effects / Q000502:physiology; D032383:Myocytes, Cardiac / Q000648:ultrastructure*; D009210:Myofibrils / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000033:anatomy & histology*; D011770:Pyruvate Kinase / Q000378:metabolism
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002950:Citrate (si)-Synthase; D011770:Pyruvate Kinase; D052281:Creatine Kinase, Mitochondrial Form; D000263:Adenylate Kinase; D002118:Calcium