Inflammatory response on injection of chitosan/GP to the brain.
Chitosan is a well-known biomaterial that, with the addition of glycerophosphate salt (GP), gels at physiological temperatures and therefore is useful for tissue engineering purposes. This study examines the procedure of injecting chitosan/ GP to the brain in order to form a gel track. The gel system and surgical technique were successful in this endeavour; however, on examining the inflammatory response to the material it was found that the chitosan/GP was wholly engulfed by macrophages within 7 days. This was determined by staining for both the gel and the macrophages, an important technique for localising injected material. The chitosan/GP-containing macrophages formed a neat tract at the lesion site, but after 45 days no chitosan/GP was found. It was concluded that, although chitosan/GP is present after implantation, it is not available for direct scaffolding in the brain.
Crompton
K E
KE
Department of Materials Engineering, Monash University, Wellington Road, Clayton, 3168, Australia.
Tomas
D
D
Finkelstein
D I
DI
Marr
M
M
Forsythe
J S
JS
Horne
M K
MK
D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects* / Q000737:chemistry; D048271:Chitosan / Q000008:administration & dosage / Q000009:adverse effects* / Q000737:chemistry; D005782:Gels / Q000378:metabolism; D005994:Glycerophosphates / Q000008:administration & dosage / Q000009:adverse effects* / Q000737:chemistry; D007150:Immunohistochemistry; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology*; D007276:Injections, Intraventricular; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials; D005782:Gels; D005994:Glycerophosphates; D048271:Chitosan