Estimation of twelve bacopa saponins in Bacopa monnieri extracts and formulations by high-performance liquid chromatography.
A simple and sensitive reversed phase high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of twelve bacopa saponins present in the extracts of the Indian Medicinal Plant, Bacopa monnieri. The separation was achieved on a reversed phase C(18) column (Luna C(18)), 5 microm by isocratic elution with 0.05 M sodium sulphate buffer (pH 2.3) and acetonitrile (68.5 : 31.5, v/v) as the mobile phase at a flow rate of 1.0 ml/min with an operating temperature of 30 degrees C. The method was validated for linearity, precision, intra- and inter-day precision and accuracy. Several Bacopa samples (plant materials, extracts and commercial formulations) were successfully analyzed. Major bacopasaponins were bacosides A(3) (3), bacopaside II (4), bacopaside I (5), bacopaside X (6), bacopasaponin C (7), bacopaside N2 (9) and the minor components were bacopasaponin F (1), bacopasaponin E (2), bacopaside N1 (8) bacopaside III (10), bacopaside IV (11) and bacopaside V (12). The total saponin content in the samples, plant materials and extracts varied from 5.1 to 22.17% and 1.47 to 66.03 mg/capsule or tablet in the commercial formulations.
Murthy
Papolu Bhargava Sriramachandra
PB
Laila Research Centre, Unit I, Phase III, Jawahar Autonagar, Vijayawada-520 007, India.
Raju
Valivarti Raghava
VR
Ramakrisana
Tummala
T
Chakravarthy
Mangu Srinivasa
MS
Kumar
Katta Vijay
KV
Kannababu
Sukala
S
Subbaraju
Gottumukkala Venkata
GV
D034681:Bacopa / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry*
D006027:Glycosides; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C454645:bacopasaponin C; C476480:bacopaside III; C476479:bacopaside IV; C442170:bacopaside I; C442169:bacopaside II