title: Value of the chlorhexidine decontamination method for recovery of nontuberculous mycobacteria from sputum samples of patients with cystic fibrosis.
abstract: The chlorhexidine method was compared to the N-acetyl-l-cysteine-NaOH-oxalic acid decontamination method currently recommended for the recovery of nontuberculous mycobacteria (NTM) from patients with cystic fibrosis. Sputum samples (n = 827) treated with chlorhexidine yielded twice as many NTM-positive cultures as those treated by the reference method (54 [6.50%] versus 27 [3.25%]; P < 0.0001) despite a higher contamination rate (20% versus 14.2%; P = 0.0017).
authors.affiliation: Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France. agnes.ferroni@nck.aphp.fr
authors.forename: Agnès
authors.initials: A
authors.lastname: Ferroni
authors.forename: Hoang
authors.initials: H
authors.lastname: Vu-Thien
authors.forename: Philippe
authors.initials: P
authors.lastname: Lanotte
authors.forename: Muriel
authors.initials: M
authors.lastname: Le Bourgeois
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sermet-Gaudelus
authors.forename: Brigitte
authors.initials: B
authors.lastname: Fauroux
authors.forename: Sophie
authors.initials: S
authors.lastname: Marchand
authors.forename: Françoise
authors.initials: F
authors.lastname: Varaigne
authors.forename: Patrick
authors.initials: P
authors.lastname: Berche
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Gaillard
authors.forename: Catherine
authors.initials: C
authors.lastname: Offredo
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D002710:Chlorhexidine / Q000494:pharmacology*; D003550:Cystic Fibrosis / Q000382:microbiology*; D003666:Decontamination / Q000379:methods*; D006801:Humans; D009161:Mycobacterium / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000382:microbiology; D019815:Oxalic Acid / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology; D013183:Sputum / Q000382:microbiology*
chemical_list: D012972:Sodium Hydroxide; D019815:Oxalic Acid; D002710:Chlorhexidine; D000111:Acetylcysteine