Effect of fluid resuscitation on ryanodine receptor in macaques with endotoxic shock.
OBJECTIVE
Our recent study demonstrated that sodium bicarbonate improved cardiac function in macaque models with early-phase endotoxic shock. In the present study, we investigated further the ryanodine receptor/calcium release-channel (RyR) and calcium pump after fluid resuscitation of macaques with early-phase endotoxic shock.


METHODS
Twenty-four anaesthetised macaques were assigned to four groups. Nineteen animals were given an intravenous dose of 2.8 mgkg(-1) lipopolysaccharide (LPS). Sixty minutes after the LPS challenge, the animals were given (i) 5 mLkg(-1) normal saline (Ns group, n = 6), (ii) 5 mLkg(-1) of 5% sodium bicarbonate (Sb group, n = 6) or (iii) 5 mLkg(-1) of 3.5% hypertonic sodium chloride (Hs group, n = 7). The control group (Co group, n = 5) received 1 mLkg(-1) normal saline and then with 5 mLkg(-1) normal saline 60 min later.


RESULTS
Endotoxin produced a reduction of the density of RyR but did not alter the affinity of RyR. Compared with normal saline, sodium bicarbonate or hypertonic saline induced a restoration of density of RyR but did not influence the affinity of RyR and the calcium pump.


CONCLUSIONS
Up-regulation of RyR performance in myocardium following administration of sodium bicarbonate contributes to the improvement of cardiac function in macaques in the early phase of endotoxic shock.
Yin
Guo Qing
GQ
The Second Hospital of Nanjing, Medical College, Southeast University, 1-1 Zhong-fu Road, Nanjing, Jiangsu 210003, PR China. yinguoq@jlonline.com
Ji
Xiao Hui
XH
Gu
Fei Rong
FR
Lu
Cheng Ping
CP
Fang
Zhi Xun
ZX
Zhao
Wei
W
Chen
Yan
Y
Shen
Ling
L
Tang
Jia Qi
JQ
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000187:drug effects* / Q000378:metabolism; D004195:Disease Models, Animal; D004926:Escherichia coli; D005260:Female; D005440:Fluid Therapy; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008251:Macaca; D008297:Male; D009206:Myocardium / Q000378:metabolism; D012151:Resuscitation / Q000379:methods*; D019837:Ryanodine Receptor Calcium Release Channel / Q000187:drug effects* / Q000378:metabolism; D012462:Saline Solution, Hypertonic / Q000494:pharmacology* / Q000627:therapeutic use; D012772:Shock, Septic / Q000209:etiology / Q000378:metabolism* / Q000628:therapy; D017693:Sodium Bicarbonate / Q000494:pharmacology* / Q000627:therapeutic use; D015854:Up-Regulation
D008070:Lipopolysaccharides; D019837:Ryanodine Receptor Calcium Release Channel; D012462:Saline Solution, Hypertonic; D017693:Sodium Bicarbonate; D000252:Calcium-Transporting ATPases