Immune protection effect of exosomes against attack of L1210 tumor cells.
We investigated the anti-tumor immunity of L1210 cell-secreted exosomes. Exosomes were purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation. Expression of H-2D and interstitial cell adhesion molecule (ICAM(-1 was investigated by solid-phase immuno-electron microscopy and expression of Hsp70 was investigated by western blotting. DBA/2 mice were immunized with a given dose of exosomes (2.5 or 5 microg(. Transmission electron microscopy revealed that L1210-derived exosomes were membrane vesicles. They were labeled by colloidal gold H-2D and ICAM-1. Western blot analysis demonstrated the presence of Hsp70 antigens in L1210 exosomes. Exosome immunization partly inhibited the growth of implanted tumor in mice. There was a significant difference between exosome groups and the control group (P < 0.05(. In conclusion, exosomes can be considered as a candidate therapeutic vaccine for leukemia.
Bu
Ning
N
Department of Hematology, Xijing Hospital, China. bnbu@163.com
Li
Qi-Ling
QL
Feng
Qi
Q
Sun
Bing-Zhong
BZ
D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage; D019496:Cancer Vaccines; D045744:Cell Line, Tumor; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D007167:Immunotherapy / Q000379:methods*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007938:Leukemia / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008811:Mice, Inbred DBA; D022142:Secretory Vesicles / Q000737:chemistry / Q000276:immunology* / Q000637:transplantation*
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D018840:HSP70 Heat-Shock Proteins; D018799:Intercellular Adhesion Molecule-1