title: Evaluation of new apolipoprotein C-II and apolipoprotein C-III automatized immunoturbidimetric kits.
abstract: BACKGROUND
  Apolipoprotein C-II and apolipoprotein C-III play an important and complex role in plasma triglycerides metabolism, respectively, as inhibitor and activator of lipoprotein lipase. Thus, they appear to be suitable markers for clinical studies of triglyceride-rich lipoproteins and related cardiovascular risk. Our aim was to evaluate, for routine analysis, the accuracy to quantify these apolipoprotein in human sera.
  
  
  METHODS
  Precision (intra- and inter-run), limit of detection and quantification, linearity, common interferents (lipids, haemoglobin, bilirubin) and reference intervals were determined according to guidelines of the French Society of Clinical Biology and ISO Norm 5725 specifications.
  
  
  RESULTS
  Intra- and inter-run CVs were respectively less than 5.0% and 7.5%. Linearities extended from 10.8 mg/L to 112.9 mg/L for apolipoprotein C-II and from 31.8 mg/L to 375.5 mg/L for apolipoprotein C-III. Haemolysis (up to 227.6 micromol/L haemoglobin) and lipemia (up to 19.3 mmol/L triglycerides) do not interfere, contrary to bilirubin, which has a positive effect above 350 micromol/L. Comparison of methods shows good agreement between immunoturbidimetric and electro-immunodiffusion methods for measuring apolipoprotein C-III in 62 samples within a wide range (n = 62, r = 0.954, y = 3.81 x -14.4).
  
  
  CONCLUSIONS
  This study shows the reliability of these kits for measuring apolipoprotein C-II and apolipoprotein C-III in human sera, and their suitability for routine analysis.
authors.affiliation: Laboratoire Central, Hôpital Sainte-Marguerite, 270 bd Sainte-Marguerite, BP 29, 13274 Marseille Cedex 09, France. alain.nicolay@pharmacie.univ-mrs.fr <alain.nicolay@pharmacie.univ-mrs.fr>
authors.forename: Alain
authors.initials: A
authors.lastname: Nicolay
authors.forename: Elise
authors.initials: E
authors.lastname: Lombard
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Arlotto
authors.forename: Vincent
authors.initials: V
authors.lastname: Saunier
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Lorec-Penet
authors.forename: Denis
authors.initials: D
authors.lastname: Lairon
authors.forename: Henri
authors.initials: H
authors.lastname: Portugal
mesh_terms: D000368:Aged; D053304:Apolipoprotein C-II / Q000097:blood*; D053305:Apolipoprotein C-III / Q000097:blood* / Q000378:metabolism*; D001322:Autoanalysis; D001331:Automation; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D012044:Regression Analysis
chemical_list: D053304:Apolipoprotein C-II; D053305:Apolipoprotein C-III; D011933:Reagent Kits, Diagnostic