[Autoantibodies against beta1 and M2 receptor in diabetic patients with refractory hypertension].
OBJECTIVE
To explore the role of the autoantibodies against M(2)-muscarinic receptor (M(2)-receptor), beta(1)-adrenergic receptor (beta(1)-receptor) in the development of diabetic with refractory hypertension.


METHODS
Serum autoantibodies against M(2) and beta(1) were detected by ELISA using synthesized epitopes of the second extracellular loop of M(2) receptor (169 - 193) and beta(1) receptor (197 - 222) in healthy controls (n = 40), diabetic patients (n = 62), diabetic patients with non-refractory hypertension (n = 55) and diabetic patients with refractory hypertension (n = 81).


RESULTS
The positive rates of the autoantibodies against M(2) receptor and beta(1) receptor were similar among healthy controls (15.0% and 17. 5%), diabetes mellitus patients (17.7% and 14.5%) and diabetic patients with non-refractory hypertension (16.4% and 12.7%) but are significantly higher in diabetic patients with refractory hypertension (64.2% and 55.6%, P < 0.01 vs. other 3 groups).


CONCLUSIONS
This finding suggests that autoimmune mechanisms might play a role in the pathogenesis of diabetic patients with refractory hypertension.
Zhao
Lin-shuang
LS
Department of Endocrinology Wuhan General Hospital of GuangZhou Military Region, Wuhan 430070, China.
Liao
Yu-hua
YH
Xiang
Guang-da
GD
Wang
Min
M
Zhou
Zi-hua
ZH
Hou
Jie
J
Le
Ling
L
Xu
Lin
L
D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000150:complications; D008297:Male; D008875:Middle Aged; D043585:Receptor, Muscarinic M2 / Q000276:immunology*; D018342:Receptors, Adrenergic, beta-1 / Q000276:immunology*
D001323:Autoantibodies; D043585:Receptor, Muscarinic M2; D018342:Receptors, Adrenergic, beta-1