Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis.
For developmental competence of mammalian embryos, dynamic epigenetic changes to both the maternally and paternally derived contributions to the genome of the zygote should be brought about in the early cleavage stages. DNA methylation is a typical epigenetic mark modified during pre-implantation development. Here, we describe how to analyze DNA methylation profiles in early-stage embryos.
Han
Yong-Mahn
YM
Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology, Daejeon.
Kim
Seok-Ho
SH
Kang
Yong-Kook
YK
D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000473:pathology*; D002417:Cattle; D003001:Cloning, Molecular; D018899:CpG Islands; D019175:DNA Methylation; D017931:DNA Primers / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D044127:Epigenesis, Genetic; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D016296:Mutagenesis; D013447:Sulfites / Q000737:chemistry / Q000494:pharmacology*
D017931:DNA Primers; D013447:Sulfites; D004262:DNA Restriction Enzymes