The visualization of hepatic vasculature by X-ray micro-computed tomography.
In this study, X-ray micro-computed tomography (CT) was used to reconstruct the fine structure macro- and microvasculature in three dimensions in contrast-enhanced rat liver samples. The subsequent application in the experimental CC531s colorectal cancer model was concurrent with results obtained from confocal microscopy in earlier studies. The en bloc stains osmium tetroxide in combination with uranyl acetate provided an excellent contrasting result for hepatic tissue after a trial of several contrasting agents. X-ray micro-CT allowed us to image the large blood vessels together with the branching sinusoids of hepatic tissue in three dimensions. Furthermore, interruption of the microvasculature was noted when rats were injected with CC531s colorectal cancer cells indicating the presence of hepatic metastases.
Ananda
Shalini
S
Australian Key Centre for Microscopy and Microanalysis, Electron Microscope Unit, The University of Sydney NSW 2006, Australia.
Marsden
Valerie
V
Vekemans
Katrien
K
Korkmaz
Emine
E
Tsafnat
Naomi
N
Soon
Lilian
L
Jones
Allan
A
Braet
Filip
F
D000792:Angiography; D000818:Animals; D001808:Blood Vessels / Q000648:ultrastructure*; D002196:Capillaries / Q000000981:diagnostic imaging; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000648:ultrastructure; D008099:Liver / Q000098:blood supply*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D014057:Tomography, X-Ray Computed / Q000379:methods*