title: Cyclosporin A stimulates apical Na+/H+ exchange in LLC-PK1/PKE20 proximal tubular cells.
abstract: Cyclosporin A (CyA) causes renal Na(+) retention which may lead to arterial hypertension. The apical Na(+)/H(+) exchanger (NHE3) is responsible for bulk proximal tubular Na(+) reabsorption. The aim of this study was to investigate the effects of CyA on the NHE3 of polarized proximal tubular cells to evaluate cellular mechanisms of CyA-associated arterial hypertension. The change of the intracellular pH (Delta-[pH](i)/min) was determined as a measure of the activity of the NHE in LLC-PK(1)/PKE(20) cells using 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF). The NHE activity was identified as the apical NHE3 since it could be inhibited by the inhibitor S3226, but not by inhibitors of the basolateral isoform (NHE1) amiloride or HOE 694. CyA stimulated the NHE3 activity dose dependently. The mean increase stimulated by relevant CyA concentrations was 61+/-11%. A 24-h application of CyA also stimulated an increase of NHE3 activity which did not seem to be mediated by an increase of NHE3 RNA expression. The less immunosuppressive derivatives cyclosporin H and cyclosporin G caused NHE3 activation as well. Carbachol and ATP, which both induce a Ca(2+) release from internal Ca(2+) stores, also increased the NHE3 activity. The Ca(2+) chelator 1,2-bis-(2-aminophenoxy)-ethane-N,N,-N',N'-tetraacetic acid tetraacetoxymethyl ester (BAPTA-AM) abolished the CyA-associated NHE3 stimulation, whereas low extracellular Ca(2+) had no effect. CyA-associated effects did not seem to be mediated via inhibition of protein kinase C (PKC). CyA had no additive effects on the angiotensin II-associated NHE3 stimulation. Concurrent application of losartan did not impair the CyA-induced NHE3 stimulation. In conclusion CyA stimulates the apical NHE3 in proximal tubular cells. This is mediated by Ca(2+) release from intracellular stores but is independent of the action of angiotensin II or PKC.
authors.affiliation: Universitäts-Kinderklinik, Mathildenstr. 1, 79106, Freiburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Epting
authors.forename: Kathrin
authors.initials: K
authors.lastname: Hartmann
authors.forename: Anna
authors.initials: A
authors.lastname: Sandqvist
authors.forename: Roland
authors.initials: R
authors.lastname: Nitschke
authors.forename: Nader
authors.initials: N
authors.lastname: Gordjani
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D015152:Blotting, Northern; D065095:Calcineurin Inhibitors; D002118:Calcium / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology*; D005456:Fluorescent Dyes; D016257:Fura-2; D006863:Hydrogen-Ion Concentration; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D018374:LLC-PK1 Cells / Q000187:drug effects / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D014662:Vasoconstrictor Agents / Q000494:pharmacology
chemical_list: D065095:Calcineurin Inhibitors; D005456:Fluorescent Dyes; D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers; D014662:Vasoconstrictor Agents; D000804:Angiotensin II; C045571:cyclosporin G; D016572:Cyclosporine; D011493:Protein Kinase C; C050025:cyclosporin H; D002118:Calcium; D016257:Fura-2