The free cytoplasmic Ca2+ levels in Duchenne muscular dystrophy lymphocytes.
An increased cellular Ca2+ content has been associated with Duchenne muscular dystrophy (DMD). However, estimates of the free cytoplasmic Ca2+ concentration ([Ca2+]i) in cells of DMD patients were not available. We compared the [Ca2+]i levels of normal and DMD peripheral blood lymphocytes and Epstein-Barr virus-transformed lymphoblasts using the novel probe, quin 2, an internally trapped fluorescent indicator. The [Ca2+]i levels of normal and DMD cells were not significantly different.
Klip
A
A
Department of Neurology, Research Institute, Hospital for Sick Children, University of Toronto, Ontario, Canada.
Elder
B
B
Ruiz-Funes
H P
HP
Buchwald
M
M
Grinstein
S
S
D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology*; D020388:Muscular Dystrophy, Duchenne / Q000473:pathology*
D002118:Calcium