Large area protein nanopatterning for biological applications.
Large area nanopatterns of functional proteins are demonstrated. A new approach to analyze atomic force microscopy height histograms is used to quantify protein and antibody binding to nanoscale patches. Arrays of nanopatches, each containing less than 40 laminin molecules, are shown to be highly functional binding close to 1 monoclonal anti-laminin IgG (site by IKVAV sequence) or 3-4 polyclonal anti-laminin IgG's per surface bound laminin. Complementary quartz crystal microbalance measurements indicate higher functionality at nanopatches than on homogeneous surfaces.
Agheli
H
H
Department of Applied Physics, Chalmers University of Technology, S-41296 Göteborg, Sweden.
Malmström
J
J
Larsson
E M
EM
Textor
M
M
Sutherland
D S
DS
D000327:Adsorption; D001665:Binding Sites; D001695:Biology / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003460:Crystallization / Q000379:methods*; D007797:Laminin / Q000737:chemistry* / Q000648:ultrastructure*; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D011485:Protein Binding; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D007797:Laminin