title: Polysaccharide differences between planktonic and biofilm-associated EPS from Pseudomonas fluorescens B52.
abstract: The polysaccharides associated with free (planktonic) and surface-attached (biofilm) cells from cultures of Pseudomonas fluorescens strain B52 were compared. Variations in the attached matrix due to surface material (glass or stainless steel) were also analyzed. Two digestion methods were used to optimize the recoveries of sugars, uronic acids and acidic substituents. The yield of analyzable material after digestion reached 90% for the material associated to the biofilms, though only 20-30% for that bound to planktonic cells. The polysaccharide(s) in the biofilm had glucuronic and guluronic acids as main components, besides rhamnose, glucose and glucosamine. The proportion of glucuronic to guluronic acid was higher in the polysaccharide(s) found in biofilms formed on stainless steel than in those on glass.
authors.affiliation: Departamento de Nutrición, Bromatología y Tecnología de Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Juliana
authors.initials: J
authors.lastname: Kives
authors.forename: Belén
authors.initials: B
authors.lastname: Orgaz
authors.forename: Carmen
authors.initials: C
authors.lastname: Sanjosé
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms; D002241:Carbohydrates / Q000032:analysis; D002852:Chromatography, Ion Exchange; D003124:Colorimetry; D005110:Extracellular Space / Q000378:metabolism; D005898:Glass; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D010933:Plankton / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D013193:Stainless Steel; D013499:Surface Properties; D014574:Uronic Acids / Q000032:analysis
chemical_list: D002241:Carbohydrates; D011135:Polysaccharides, Bacterial; D014574:Uronic Acids; D013193:Stainless Steel