title: Gene duplication event in family 12 glycosyl hydrolase from Phytophthora spp.
abstract: A total of 18 paralogs of xyloglucan-specific endoglucanases (EGLs) from the glycosyl hydrolase family 12 were identified and characterized in Phytophthora sojae and Phytophthora ramorum. These genes encode predicted extracellular enzymes, with sizes ranging from 189 to 435 amino acid residues, that would be capable of hydrolyzing the xyloglucan component of the host cell wall. In two cases, four and six functional copies of these genes were found in tight succession within a region of 5 and 18 kb, respectively. The overall gene copy number and relative organization appeared well conserved between P. sojae and P. ramorum, with apparent synteny in this region of their respective genomes. Phylogenetic analyses of Phytophthora endoglucanases of family 12 and other known members of EGL 12, revealed a close relatedness with a fairly conserved gene sub-family containing, among others, sequences from the fungi Emericella desertorum and Aspergillus aculeatus. This is the first report of family 12 EGLs present in plant pathogenic eukaryotes.
authors.affiliation: Department of Natural Resource Sciences and Landscape Architecture, University of Maryland, College Park, 20742, USA.
authors.forename: Stefano
authors.initials: S
authors.lastname: Costanzo
authors.forename: M D
authors.initials: MD
authors.lastname: Ospina-Giraldo
authors.forename: K L
authors.initials: KL
authors.lastname: Deahl
authors.forename: C J
authors.initials: CJ
authors.lastname: Baker
authors.forename: Richard W
authors.initials: RW
authors.lastname: Jones
mesh_terms: D020418:Algal Proteins / Q000145:classification / Q000235:genetics; D000595:Amino Acid Sequence; D002480:Cellulase / Q000235:genetics; D048028:DNA, Algal / Q000737:chemistry / Q000235:genetics; D020440:Gene Duplication; D015870:Gene Expression / Q000235:genetics; D006026:Glycoside Hydrolases / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny; D010838:Phytophthora / Q000201:enzymology / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods; D017386:Sequence Homology, Amino Acid
chemical_list: D020418:Algal Proteins; D048028:DNA, Algal; D006026:Glycoside Hydrolases; D002480:Cellulase