title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection.
abstract: As part of an investigation aimed at illuminating the possibilities and limits of the HOPE-fixation and paraffin-embedding technique we here describe a novel procedure which was developed in order to combine the benefits of the HOPE-technique with the capabilities of laser microdissection. The presented procedure avoids the need for amplification of template-RNA thus facilitating reliable and reproducible results. The excellent preservation of nucleic acids, proteins, and morphology in HOPE-fixed, paraffin-embedded tissues enhances the molecular applications available to date with materials acquired by laser microdissection when compared to formalin fixed, paraffin-embedded tissues, thus substantially extending the methodological panel in tissue based research.
authors.affiliation: Clinical and Experimental Pathology, Research Center Borstel, Germany. tgoldmann@fz-borstel.de
authors.forename: Torsten
authors.initials: T
authors.lastname: Goldmann
authors.forename: Renate
authors.initials: R
authors.lastname: Burgemeister
authors.forename: Ulrich
authors.initials: U
authors.lastname: Sauer
authors.forename: Siegfried
authors.initials: S
authors.lastname: Loeschke
authors.forename: Dagmar Silvia
authors.initials: DS
authors.lastname: Lang
authors.forename: Detlev
authors.initials: D
authors.lastname: Branscheid
authors.forename: Peter
authors.initials: P
authors.lastname: Zabel
authors.forename: Ekkehard
authors.initials: E
authors.lastname: Vollmer