Evaluation of new apolipoprotein C-II and apolipoprotein C-III automatized immunoturbidimetric kits.
BACKGROUND
Apolipoprotein C-II and apolipoprotein C-III play an important and complex role in plasma triglycerides metabolism, respectively, as inhibitor and activator of lipoprotein lipase. Thus, they appear to be suitable markers for clinical studies of triglyceride-rich lipoproteins and related cardiovascular risk. Our aim was to evaluate, for routine analysis, the accuracy to quantify these apolipoprotein in human sera.


METHODS
Precision (intra- and inter-run), limit of detection and quantification, linearity, common interferents (lipids, haemoglobin, bilirubin) and reference intervals were determined according to guidelines of the French Society of Clinical Biology and ISO Norm 5725 specifications.


RESULTS
Intra- and inter-run CVs were respectively less than 5.0% and 7.5%. Linearities extended from 10.8 mg/L to 112.9 mg/L for apolipoprotein C-II and from 31.8 mg/L to 375.5 mg/L for apolipoprotein C-III. Haemolysis (up to 227.6 micromol/L haemoglobin) and lipemia (up to 19.3 mmol/L triglycerides) do not interfere, contrary to bilirubin, which has a positive effect above 350 micromol/L. Comparison of methods shows good agreement between immunoturbidimetric and electro-immunodiffusion methods for measuring apolipoprotein C-III in 62 samples within a wide range (n = 62, r = 0.954, y = 3.81 x -14.4).


CONCLUSIONS
This study shows the reliability of these kits for measuring apolipoprotein C-II and apolipoprotein C-III in human sera, and their suitability for routine analysis.
Nicolay
Alain
A
Laboratoire Central, Hôpital Sainte-Marguerite, 270 bd Sainte-Marguerite, BP 29, 13274 Marseille Cedex 09, France. alain.nicolay@pharmacie.univ-mrs.fr <alain.nicolay@pharmacie.univ-mrs.fr>
Lombard
Elise
E
Arlotto
Emmanuelle
E
Saunier
Vincent
V
Lorec-Penet
Anne-Marie
AM
Lairon
Denis
D
Portugal
Henri
H
D000368:Aged; D053304:Apolipoprotein C-II / Q000097:blood*; D053305:Apolipoprotein C-III / Q000097:blood* / Q000378:metabolism*; D001322:Autoanalysis; D001331:Automation; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D012044:Regression Analysis
D053304:Apolipoprotein C-II; D053305:Apolipoprotein C-III; D011933:Reagent Kits, Diagnostic