Towards biochips using microstructured optical fiber sensors.
In this paper we present the first incorporation of a microstructured optical fiber (MOF) into biochip applications. A 16-mm-long piece of MOF is incorporated into an optic-fluidic coupler chip, which is fabricated in PMMA polymer using a CO(2) laser. The developed chip configuration allows the continuous control of liquid flow through the MOF and simultaneous optical characterization. While integrated in the chip, the MOF is functionalized towards the capture of a specific single-stranded DNA string by immobilizing a sensing layer on the microstructured internal surfaces of the fiber. The sensing layer contains the DNA string complementary to the target DNA sequence and thus operates through the highly selective DNA hybridization process. Optical detection of the captured DNA was carried out using the evanescent-wave-sensing principle. Owing to the small size of the chip, the presented technique allows for analysis of sample volumes down to 300 nL and the fabrication of miniaturized portable devices.
Rindorf
Lars
L
COM.DTU, Department of Communication, Optics and Materials, Technical University of Denmark, 2800, Kongens Lyngby, Denmark. lhr@com.dtu.dk
Høiby
Poul Erik
PE
Jensen
Jesper Bo
JB
Pedersen
Lars Hagsholm
LH
Bang
Ole
O
Geschke
Oliver
O
D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry; D015342:DNA Probes; D005336:Fiber Optic Technology / Q000379:methods*; D007834:Lasers; D008832:Microchemistry; D044085:Microfluidics / Q000379:methods*; D055100:Optical Fibers
D015342:DNA Probes; D004247:DNA