title: Extraction and detection of mRNA from horsehair.
abstract: After RNA extraction from horsehair shafts and roots, the mRNAs of beta-actin, muscle-type phosphofructokinase, and transforming growth factor-beta1 were detected by reverse transcription polymerase chain reaction assay. Low amounts of RNA were present in the horsehair. These specific mRNA transcripts were readily detected when more than three hair roots were used. However, detection of the mRNA transcripts was difficult in the hair shaft. These findings indicate that the small amounts of residual RNA in horsehair roots can be utilized as samples for molecular biological analysis.
authors.affiliation: Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Sato
authors.forename: Go
authors.initials: G
authors.lastname: Sato
authors.forename: Youko
authors.initials: Y
authors.lastname: Shoji
authors.forename: Takuya
authors.initials: T
authors.lastname: Itou
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000199:Actins / Q000032:analysis / Q000235:genetics; D000818:Animals; D006197:Hair / Q000737:chemistry*; D006736:Horses / Q000235:genetics / Q000378:metabolism; D025221:Phosphofructokinases / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000235:genetics
chemical_list: D000199:Actins; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D025221:Phosphofructokinases