A consensus epitope prediction approach identifies the breadth of murine T(CD8+)-cell responses to vaccinia virus.
The value of predictive algorithms for identifying CD8+ T (T(CD8+))-cell epitopes has not been adequately tested experimentally. Here we demonstrate that conventional bioinformatic methods predict the vast majority of T(CD8+)-cell epitopes derived from vaccinia virus WR strain (VACV-WR) in the H-2(b) mouse model. This approach reveals the breadth of T-cell responses to vaccinia, a widely studied murine viral infection model, and may provide a tool for developing comprehensive antigenic maps of any complex pathogen.
Moutaftsi
Magdalini
M
La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill Street, Suite 326, San Diego, California 92109, USA.
Peters
Bjoern
B
Pasquetto
Valerie
V
Tscharke
David C
DC
Sidney
John
J
Bui
Huynh-Hoa
HH
Grey
Howard
H
Sette
Alessandro
A
D000465:Algorithms; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D019295:Computational Biology / Q000379:methods*; D018604:Epitope Mapping / Q000379:methods*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014616:Vaccinia virus / Q000276:immunology*
D018984:Epitopes, T-Lymphocyte