title: [Rapid analysis of antiepileptic drugs in human plasma by micellar electrokinetic capillary chromatography].
abstract: OBJECTIVE
  To develop a rapid and feasible method based on micellar electrokinetic capillary chromatography (MECC) for the simultaneous determination of antiepileptic drugs (AEDs)--phenytoin (PHT), phenobarbital (PB), carbamazepine (CBZ), primidone (PRM) and clonazepam (CZP) in human plasma.
  
  
  METHODS
  Several factors that impact the separation of AEDs with MECC were investigated, such as concentration of sodium dodecyl sulfate (SDS), buffer compositions, pH, organic modifier, internal diameter and temperature, and an optimized MECC running condition was obtained the running buffer consisted of 8 mmol x L(-1) phosphate, 3 mmol x L(-1) sodium tetraborate, and 50 mmol x L(-1) sodium dodecylsulfate (SDS) (pH 8.0), containing acetonitrile (ACN) (18%) as organic modifier. Detection at 210 nm, run at 25 kV at 30 degrees C in a untreated fused silica capillary (50/45.5 cm length, 50 microm ID).
  
  
  RESULTS
  The reproducibility of both migration time and relative peak area with MECC analysis were appropriate for the intra- and inter-assay coefficients. The evaluated drugs concentration intervals of PRM 1.0-40.0 microg x mL(-1), PB 1.0-60.0 microg x mL(-1), PHT 1.0-40.0 microg x mL(-1), CBZ 1.0-40.0 microg x mL(-1), CZP 0.2-8.0 microg x mL(-1) were linear with correlation coefficients higher than 0.999 1, and coefficients of the variation of the points of the calibration curve lower than 10%. The recoveries of AEDs varied from 80.0% to 100.0%, depending on the drug, with coefficients of the variation lower than 10.0%.
  
  
  CONCLUSIONS
  The MECC technique is showed to be rapid, simple, efficient and low cost when applied to monitoring therapeutic drugs in patient treated with a combination of PHT and other AEDs such as hepatic enzyme-inducing agents.
authors.affiliation: Center for Instrumental Analysis of China Pharmaceutical University, Nanjing.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Rui
authors.forename: Jin-heng
authors.initials: JH
authors.lastname: Li
authors.forename: Bing-ren
authors.initials: BR
authors.lastname: Xiang
mesh_terms: D000927:Anticonvulsants / Q000097:blood*; D002021:Buffers; D002220:Carbamazepine / Q000097:blood; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D002998:Clonazepam / Q000097:blood; D004827:Epilepsy / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010634:Phenobarbital / Q000097:blood; D010672:Phenytoin / Q000097:blood; D011324:Primidone / Q000097:blood; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate
chemical_list: D000927:Anticonvulsants; D002021:Buffers; D011324:Primidone; D002220:Carbamazepine; D012967:Sodium Dodecyl Sulfate; D002998:Clonazepam; D010672:Phenytoin; C028913:dodecyl sulfate; D010634:Phenobarbital