Isolation of human eosinophils from peripheral blood using hypotonic lysis and centrifugation.
We describe an alternative method for rapidly purifying viable and structurally intact eosinophils from human peripheral blood using controlled hypotonic lysis and centrifugation. This method has significant advantages in that it does not require the use of density gradients, antibodies, magnetic beads, or ammonium chloride lysis procedures.
Samoszuk
Michael
M
Department of Pathology and Laboratory Medicine, University of California-Irvine, Orange, USA. mike.samoszuk@roche.com
D002469:Cell Separation; D002498:Centrifugation; D004804:Eosinophils / Q000166:cytology*; D006461:Hemolysis; D006801:Humans; D007038:Hypotonic Solutions
D007038:Hypotonic Solutions