title: Protein electrophoresis in cranes with presumed insect bite.
abstract: BACKGROUND
  Serum protein electrophoresis (SPE) has emerged as a potentially valuable diagnostic tool in avian medicine; yet, there is limited information regarding SPE in cranes. Since 2000, 20 cases of unilateral periocular or facial soft tissue swelling, blepharitis, feather loss, and ocular or nasal discharge attributed to insect bite hypersensitivity were observed in cranes from a captive breeding center. SPE may be useful for evaluating these lesions.
  
  
  OBJECTIVE
  The aim of this study was to characterize the inflammatory response in cranes with hypersensitivity reactions using SPE.
  
  
  METHODS
  Serum samples from 7 cranes diagnosed with hypersensitivity reactions were submitted to a diagnostic laboratory for agarose gel electrophoresis. Results were compared to those in control serum samples obtained from the same cranes during routine physical examination, when they were clinically healthy.
  
  
  RESULTS
  Total protein and a- and g-globulin concentrations were significantly increased and albumin/globulin ratios were significantly decreased in serum samples from cranes with hypersensitivity lesions compared with control samples.
  
  
  CONCLUSIONS
  Using SPE, we documented changes in protein fraction concentrations in cranes with clinical signs of hypersensitivity. The increase in alpha- and gamma-globulin concentrations suggested inflammation and antigenic stimulation, consistent with a Type I hypersensitivity reaction.
authors.affiliation: Department of Veterinary Services, International Crane Foundation, Baraboo, WI, USA. hartup@savingcranes.org
authors.forename: Barry K
authors.initials: BK
authors.lastname: Hartup
authors.forename: Carrie A
authors.initials: CA
authors.lastname: Schroeder
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D001717:Birds; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006967:Hypersensitivity / Q000378:metabolism / Q000473:pathology*; D007299:Insect Bites and Stings / Q000378:metabolism / Q000473:pathology*; D008297:Male
chemical_list: D001798:Blood Proteins