Beneficial effect of erythropoietin administration on murine infection with Trypanosoma congolense.
The effect of erythropoietin treatment on Trypanosoma congolense infection in mice was studied. Survival rates of mice were dramatically improved by treatment with recombinant human erythropoietin (r-hu-EPO; 5,000 U/kg) when infected with 1,000 cells of T. congolense IL3000 (P < 0.05). All the untreated mice infected with T. congolense IL3000 died by day 9 of infection; however, 100%, 50%, and 25% of the mice treated with r-hu-EPO for 8 days survived to day 20, day 40, and day 60 of the parasitical infection, respectively. Anti-8-hydroxy-2'-deoxyguanosine antibody, a biomarker for oxidative damage of DNA, yielded positive reactions in the cytoplasm of the parasites recovered from the mice treated with r-hu-EPO. These results, taken together, indicate that erythropoietin administration is effective for the treatment of T. congolense infection.
Suzuki
Tomoko
T
Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
Ueta
Yoshiko Yanagimoto
YY
Inoue
Noboru
N
Xuan
Xuenan
X
Saitoh
Hideki
H
Suzuki
Hiroshi
H
D000740:Anemia / Q000517:prevention & control; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004921:Erythropoietin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D020869:Gene Expression Profiling; D006400:Hematocrit; D051379:Mice; D008810:Mice, Inbred C57BL; D018512:Parasitemia / Q000150:complications / Q000188:drug therapy* / Q000469:parasitology; D010419:Pentamidine / Q000494:pharmacology; D016133:Polymerase Chain Reaction / Q000379:methods; D017467:Receptors, Erythropoietin / Q000096:biosynthesis / Q000187:drug effects; D011994:Recombinant Proteins; D016019:Survival Analysis; D013997:Time Factors; D014348:Trypanosoma congolense / Q000187:drug effects* / Q000302:isolation & purification; D014353:Trypanosomiasis, African / Q000188:drug therapy* / Q000401:mortality / Q000469:parasitology
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D015342:DNA Probes; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins; D004921:Erythropoietin; D010419:Pentamidine