Determination of bovine serum low-density lipoprotein cholesterol using the N-geneous method.
The N-geneous method is a recently developed method for determination of low-density lipoprotein cholesterol (LDL-C) in human serum. In the present study, we attempted to adapt this method to bovine serum. The values of LDL-C obtained using the N-geneous method were highly correlated with those from the method using ultracentrifugation and heparin sepharose affinity chromatography (r = 0.934, p < 0.001). The reproducibility of this method was acceptable (intra-assay CV 4.2%, inter-assay CV 7.6%) for clinical use. Using the N-geneous method, serum LDL-C was evaluated in cows around parturition, and in cows with fatty liver induced by fasting. The concentration of LDL-C decreased significantly in cows close to parturition. A reduced concentration of LDL-C was also observed in cows with fatty liver. In both cases, the changes of LDL-C were similar to those of apolipoprotein B (apoB)-100, and the values of LDL-C were highly correlated (r = 0.876, p < 0.001) with those of apoB-100. These results suggest that the concentration of LDL-C reflects the level of apoB-100. The N-geneous method is simple and rapid, and might to be a useful tool to elucidate the clinical significance of LDL-C in bovine serum.
Miyamoto
T
T
Department of Production Disease, National Institute of Animal Health, Kannondai, Tsukuba, Ibaraki, Japan. miyomoto@affrc.go.jp
Sugiyama
Y
Y
Suzuki
J
J
Oohashi
T
T
Takahashi
Y
Y
D000818:Animals; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000097:blood; D002417:Cattle / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood*; D002847:Chromatography, Agarose / Q000662:veterinary; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005234:Fatty Liver / Q000097:blood; D005260:Female; D036801:Parturition / Q000097:blood; D011247:Pregnancy; D011933:Reagent Kits, Diagnostic / Q000592:standards / Q000662:veterinary*; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation / Q000662:veterinary
D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D008078:Cholesterol, LDL; D005230:Fatty Acids, Nonesterified; D011933:Reagent Kits, Diagnostic; D014280:Triglycerides