title: Double immunofluorescence shows coexpression of Bcl-x with GFAP in a variety of glial lesions.
abstract: Bcl-x is an important member of the bcl-2 family of proteins that has been shown to be expressed by both native nervous system tissue and several nervous system tumors. Its anti-apoptotic activity is believed to contribute to nervous system tumorigenesis. We seek to compare the staining characteristics of Bcl-x and GFAP in various neuronal and glial lesions, both neoplastic and non-neoplastic. We also use a double immunofluorescence technique to assess for coexpression of Bcl-x and GFAP by the same lesional cells. Forty cases of brain tumors and reactive brain conditions were reviewed. The former included astrocytomas, GBMs, ependymomas, oligodendrogliomas, gangliogliomas, subependymomas and neurocytomas. The latter included cases of gliosis, cerebritis and mesial temporal sclerosis. Immunohistochemistry for Bcl-x and GFAP was performed. Double immunofluorescent labeling using antibodies to both GFAP and Bcl-x was also carried out. Expression of Bcl-x closely follows that of GFAP with strong expression in both reactive astrocytes and astrocytomas. There is more focal expression in other gliomas. Immunostaining for Bcl-x is generally more intense and distinct, compared to that for GFAP. Expression of both GFAP and Bcl-x is more focal in oligodendrogliomas, with staining of mainly intervening astrocytic processes. Double immunolabelling confirms the coexpression of Bcl-x and GFAP in various gliomas and reactive brain conditions. As immunostaining for Bcl-x is generally more distinct and intense than that for GFAP, it may serve as a useful alternative to help highlight glial cells in selected diagnostic settings.
authors.affiliation: Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, National University Hospital, Lower Kent Ridge Road, Singapore, 119074, Singapore. pattankb@nus.edu.sg
authors.forename: Kong-Bing
authors.initials: KB
authors.lastname: Tan
authors.forename: Hui-Keng
authors.initials: HK
authors.lastname: Magdalene Koh
authors.forename: Soo-Yong
authors.initials: SY
authors.lastname: Tan
mesh_terms: D001253:Astrocytes / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D005911:Gliosis / Q000378:metabolism*; D006801:Humans; D008581:Meningitis / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D051020:bcl-X Protein