title: Multi-enzyme one-pot strategy for the synthesis of sialyl Lewis X-containing PSGL-1 glycopeptide.
abstract: An enzymatic one-pot three-step glycosylation strategy was developed for the synthesis of sLex moiety of truncated PSGL-1 glycopeptide with and without sulfation. The method provided an efficient way to afford complex glycopeptides in a semi-preparative scale without further complicated and time-consuming purification process in each glycosylation step.
authors.affiliation: Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Kuo-Ting
authors.initials: KT
authors.lastname: Huang
authors.forename: Bing-Ching
authors.initials: BC
authors.lastname: Wu
authors.forename: Chang-Ching
authors.initials: CC
authors.lastname: Lin
authors.forename: Shih-Chi
authors.initials: SC
authors.lastname: Luo
authors.forename: Chinpan
authors.initials: C
authors.lastname: Chen
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Lin
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D004798:Enzymes / Q000378:metabolism*; D005647:Fucosyltransferases / Q000378:metabolism; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009237:N-Acetyllactosamine Synthase / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen; D013431:Sulfates / Q000737:chemistry / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism
chemical_list: D004798:Enzymes; D006020:Glycopeptides; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D013431:Sulfates; D014443:Tyrosine; D005647:Fucosyltransferases; C435083:fucosyltransferase V; D009237:N-Acetyllactosamine Synthase; D000469:Alkaline Phosphatase