title: [Differentiation of transplanted mouse c-Kit+lin- bone marrow cells into hepatocytes in vitro].
abstract: OBJECTIVE
  To ascertain whether mouse c-Kit(+)Lin- bone marrow cells have the potential of hepatic stem cells.
  
  
  METHODS
  c-Kit(+)lin- bone marrow cells were isolated and purified by magnetic-activated cell sorting (MACS) from BALB/C male donor mice, and immediately transplanted into age-matched BALB/C syngeneic female mice with 35-Gy total liver irradiation. The recipients were sacrificed 1 month after the transplantation for pathological observation of the liver morphology. The presence of Y-chromosome was examined in the liver cells of the recipient by in situ hybridization (ISH), and alpha-fetoprotein (AFP) and albumin in the cells were detected by immunohistochemistry.
  
  
  RESULTS
  The hepatocytes positive for Sry gene on Y-chromosome were identified 1 month after transplantation, and immunohistochemistry for AFP and albumin confirmed that the donor mice-derived cells were hepatocytes.
  
  
  CONCLUSIONS
  c-Kit(+)lin- bone marrow cells have the potential of hepatic stem cells, which can reside and differentiate into hepatocytes in the liver after transplantation. c-Kit(+)lin- bone marrow cells can be used as the source cells of cell transplantation for liver disease.
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.forename: Cai-xian
authors.initials: CX
authors.lastname: Liao
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Hao-sheng
authors.initials: HS
authors.lastname: Jin
authors.forename: Yuan-fei
authors.initials: YF
authors.lastname: Tan
authors.forename: Jun
authors.initials: J
authors.lastname: Su
authors.forename: Chun-xing
authors.initials: CX
authors.lastname: Zhang
authors.forename: Shou-hua
authors.initials: SH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation; D005260:Female; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D039902:Multipotent Stem Cells / Q000378:metabolism / Q000637:transplantation*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D011897:Random Allocation; D014916:Whole-Body Irradiation; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D000509:alpha-Fetoproteins; D019009:Proto-Oncogene Proteins c-kit