title: [Application of serum protein fingerprint model and support vector machine in diagnosis of thyroid cancer].
abstract: OBJECTIVE
  To detect new biomarkers and to establish a serum protein fingerprint model for early detection and diagnosis of thyroid cancer.
  
  
  METHODS
  The serum samples of 40 thyroid cancer patients, 9 thyroid adenoma patient, and 32 healthy individuals were randomly divided into 2 sets: training set (n = 66, including 32 thyroid cancer patients, 9 thyroid adenoma patients, and 25 healthy individuals) and test set (n = 15). The serum protein were bound to WCX2 chip and tested by surface enhanced laser desorption/ionization time of flight-mass spectrometry (SELDI-TOF-MS). The data of spectra were analyzed by support vector machine (SVM) to establish a diagnostic model.
  
  
  RESULTS
  The detective model combined with 3 biomarkers could differentiate the serum of thyroid cancer from that of healthy individual with a specificity of 86% and a sensitivity of 100%. The diagnostic model combined with 3 biomarkers could differentiate thyroid cancer from thyroid adenoma with a specificity of 88.9% and a sensitivity of 96.9%. The positive predictive value to differentiate papillary thyroid carcinoma from the thyroid cancer of other types was 97%, and the positive predictive value of thyroid carcinoma of other pathological types was 71%.
  
  
  CONCLUSIONS
  The combination of SELDI with bioinformatics tools is a novel, effective, and highly specific and sensitive method for thyroid cancer detection and diagnosis.
authors.affiliation: Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China. wjiaxiang@zzu.edu.cn
authors.forename: Jia-xiang
authors.initials: JX
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Ying-zhong
authors.initials: YZ
authors.lastname: Fan
authors.forename: Qiu-liang
authors.initials: QL
authors.lastname: Liu
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhang
authors.forename: Jie-kai
authors.initials: JK
authors.lastname: Yu
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D006801:Humans; D010449:Peptide Mapping / Q000379:methods*; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013964:Thyroid Neoplasms / Q000097:blood / Q000175:diagnosis*
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D020543:Proteome