The clustering of four subfamilies of satellite DNA at individual chromosome ends in Silene latifolia.
The satellite DNA (satDNA) on the ends of chromosomes has been isolated and characterized in the dioecious plant Silene latifolia. BAC clones containing large numbers of repeat units of satDNA in a tandem array were isolated to examine the clustering of the repeat units. satDNA repeat units were purified from each isolated BAC clone and sequenced. To investigate pairwise similarities among the repeat units, a phylogenetic tree was constructed using the neighbor-joining algorithm. The repeat units derived from 7 BAC clones were grouped into SacI, KpnI, #11F02, and #16E07 subfamilies. The SacI and KpnI subfamilies have been reported previously. Multicolored fluorescence in situ hybridization (FISH) using SacI or KpnI subfamily probes resulted in different signal intensities and locations at the chromosomal ends, indicating that each chromosomal end has a unique composition of subfamilies of satDNA. For example, the p arm of the X chromosome exhibited signal composition similar to that on the pseudo autosomal region (PAR) of the Y chromosome, but not to that on the q arm of the X chromosome. The satDNA has not been completely homogenized in the S. latifolia genome. Each subfamily is available for a probe of FISH karyotyping.
Kazama
Yusuke
Y
Integrated Bioscience, Graduate School of Frontier Sciences, University of Tokyo, Japan. ykaze@riken.jp
Sugiyama
Ryuji
R
Suto
Yumiko
Y
Uchida
Wakana
W
Kawano
Shigeyuki
S
D001483:Base Sequence; D002874:Chromosome Mapping; D022004:Chromosome Structures / Q000378:metabolism*; D022202:Chromosomes, Artificial, Bacterial / Q000378:metabolism; D032461:Chromosomes, Plant; D016384:Consensus Sequence; D004276:DNA, Satellite; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D029751:Silene / Q000235:genetics*
D004276:DNA, Satellite