title: Uptake of C60 by human monocyte macrophages, its localization and implications for toxicity: studied by high resolution electron microscopy and electron tomography.
abstract: Despite great interest in the engineering applications of carbon-based nanoparticles, recent studies have raised concerns about their potential toxicity and safety. The release of C(60) into the environment has been suggested to be a potential risk with possible ecological implications. Here we evaluate energy-filtered transmission electron microscopy (EFTEM) and scanning transmission electron microscopy (STEM)-based electron tomography as techniques for imaging the three-dimensional (3-D) distribution of nanoparticles within cells. Our aim was to establish if human monocyte macrophages internalise nanoparticles and to assess whether nanoparticles are modified by cells following uptake. Using these techniques we were able to show a marked increased in the amount of information gained from 3-D imaging. 3-D electron tomography revealed several sub-cellular compartments containing C(60) within the cell: secondary lysosomes, along the outer and nuclear membrane and most notably inside the nucleus of the cell. Using EFTEM and STEM-based techniques we were able to visualize cell structures such as membranes, the mitochondria, ribosomes and the nucleus, without the need for traditional staining techniques. In particular we demonstrate the potential of electron tomography for whole cell studies to enable 3-D distributions of particles within cells. The concentrations of C(60) used in this study were not toxic and were chosen to study which sub-cellular compartments accumulated C(60). Knowledge of the sites of accumulation of nanoparticles will allow us to predict vulnerability if the nanoparticles can generate free radicals.
authors.affiliation: The Nanoscience Centre, University of Cambridge, 11 JJ Thompson Avenue, Cambridge CB3 OFF, UK. aep30@cam.ac.uk
authors.forename: Alexandra E
authors.initials: AE
authors.lastname: Porter
authors.forename: Karin
authors.initials: K
authors.lastname: Muller
authors.forename: Jeremy
authors.initials: J
authors.lastname: Skepper
authors.forename: Paul
authors.initials: P
authors.lastname: Midgley
authors.forename: Mark
authors.initials: M
authors.lastname: Welland
mesh_terms: D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005612:Freeze Drying; D037741:Fullerenes / Q000494:pharmacology*; D006801:Humans; D021621:Imaging, Three-Dimensional; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D046032:Microscopy, Energy-Filtering Transmission Electron; D009000:Monocytes / Q000166:cytology; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D014054:Tomography
chemical_list: D037741:Fullerenes