title: High-resolution NMR spectroscopy of a GPCR in aligned bicelles.
abstract: Solid-state NMR spectra with single-site resolution of CXCR1, a G protein-coupled receptor (GPCR), were obtained in magnetically aligned phospholipid bicelles. These results demonstrate that GPCRs in phospholipid bilayers are suitable samples for structure determination by solid-state NMR. The spectra also enable studies of drug-receptor interactions.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0307, USA.
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Stefan
authors.initials: S
authors.lastname: Prytulla
authors.forename: Anna A
authors.initials: AA
authors.lastname: De Angelis
authors.forename: Jonathan Miles
authors.initials: JM
authors.lastname: Brown
authors.forename: Hans
authors.initials: H
authors.lastname: Kiefer
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Opella
mesh_terms: D001665:Binding Sites; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000378:metabolism; D023062:Receptors, Interleukin-8A / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008051:Lipid Bilayers; D043562:Receptors, G-Protein-Coupled; D023062:Receptors, Interleukin-8A; D019204:GTP-Binding Proteins