[Study on association of CTLA4 gene polymorphism with Grave's disease in Guangxi Zhuang nationality population].
OBJECTIVE
To investigate the relationship between the polymorphic (AT)n repeats in 3ountranslated region of exon 4 of CTLA4 gene [CTLA4(AT)n] and Graveso disease (GD) in Zhuang nationality population of Guangxi province.


METHODS
The studied groups comprised 48 patients with GD and 44 normal controls. Amplification of target DNA was carried out by polymerase chain reaction (PCR). The amplified products were run by 8% polyacrylamide gel electrophoresis, and then followed by 0.1% silver staining. Some of amplified products were sequenced directly.


RESULTS
Nineteen alleles of CTLA4 gene microsatellite polymorphism were found in Guangxi Zhuang nationality individuals. The 106 bp long allele was apparently increased in patients with GD of Zhuang nationality but not in healthy controls (P< 0.05).


CONCLUSIONS
CTLA4 gene microsatellite polymorphism is strongly associated with Graveso disease in Zhuang nationality population of Guangxi province. CTLA4(AT)n 106 bp may be the susceptible gene in GD patients of Zhuang nationality in Guangxi; 19 alleles of CTLA4 gene microsatellite polymorphism were found in Guangxi Zhuang nationality individuals.
Liang
Xing-huan
XH
Department of Endocrinology, the First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, 530021 PR China. Lxhcnnn@163.com
Qin
Ying-fen
YF
Ma
Yan
Y
Xie
Xin-rong
XR
Xie
Kai-qing
KQ
Luo
Zuo-jie
ZJ
D000328:Adult; D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D060908:CTLA-4 Antigen; D002681:China; D018900:Dinucleotide Repeats / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006111:Graves Disease / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
D015703:Antigens, CD; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human