TSGA10 prevents nuclear localization of the hypoxia-inducible factor (HIF)-1alpha.
The hypoxia-inducible factor (HIF)-1 is a transcriptional regulator of genes involved in oxygen homeostasis. We previously described testis-specific isoforms of HIF-1alpha (mHIF-1alphaI.1 and hHIF-1alphaTe). Using mHIF-1alpha exon I.1 knock-out mice we confirmed the specific expression of mHIF-1alphaI.1 in the sperm tail. A protein-protein interaction between HIF-1alpha and the testis specific gene antigen 10 (TSGA10) was identified by yeast two-hybrid screening. TSGA10 is expressed in testis but also in other organs and malignant tissues. Immunofluorescence analysis indicated that the C-terminal part of TSGA10 accumulates in the midpiece of spermatozoa, where it co-localizes with HIF-1alpha. HIF-1alpha nuclear localization and HIF-1 transcriptional activity were significantly affected by overexpressed TSGA10.
Hägele
Sonja
S
Cell Physiology Group, Martin-Luther University of Halle, Magdeburger Strasse 2, D-06097 Halle, Germany.
Behnam
Babak
B
Borter
Emanuela
E
Wolfe
Jonathan
J
Paasch
Uwe
U
Lukashev
Dmitriy
D
Sitkovsky
Michail
M
Wenger
Roland H
RH
Katschinski
Dörthe M
DM
D021581:Active Transport, Cell Nucleus; D000818:Animals; D015687:Cell Hypoxia; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003598:Cytoskeletal Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011485:Protein Binding; D029589:Seminal Plasma Proteins / Q000235:genetics / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism
D003598:Cytoskeletal Proteins; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D029589:Seminal Plasma Proteins; C493239:Tsga10 protein, mouse