title: Isolation of human eosinophils from peripheral blood using hypotonic lysis and centrifugation.
abstract: We describe an alternative method for rapidly purifying viable and structurally intact eosinophils from human peripheral blood using controlled hypotonic lysis and centrifugation. This method has significant advantages in that it does not require the use of density gradients, antibodies, magnetic beads, or ammonium chloride lysis procedures.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of California-Irvine, Orange, USA. mike.samoszuk@roche.com
authors.forename: Michael
authors.initials: M
authors.lastname: Samoszuk
mesh_terms: D002469:Cell Separation; D002498:Centrifugation; D004804:Eosinophils / Q000166:cytology*; D006461:Hemolysis; D006801:Humans; D007038:Hypotonic Solutions
chemical_list: D007038:Hypotonic Solutions