title: Low dose radiation overcomes diabetes-induced suppression of hippocampal neuronal cell proliferation in rats.
abstract: We investigated the effect of low dose radiation on diabetes induced suppression of neurogenesis in the hippocampal dentate gyrus of rat. After 0.01 Gy, 0.1 Gy, 1 Gy and 10 Gy radiation was delivered, the dentate gyrus of hippocampus of streptozotocin (STZ)-induced diabetic rats were evaluated using immunohistochemistry for 5-bromo-2-deoxyuridine (BrdU), caspase-3, and terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL) staining. The number of BrdU positive cells in the non-diabetic rats, diabetic rats without radiation, diabetic rats with 0.01 Gy radiation, diabetic rats with 0.1 Gy radiation, diabetic rats with 1 Gy radiation and diabetic rats with 10 Gy radiation were 55.4+/-8.5/mm2, 33.3+/-6.4/mm2, 67.7+/-10.5/mm2, 66.6+/-10.0/mm2, 23.5+/-6.3/mm2 and 14.3+/-7.2/mm2, respectively. The number of caspase-3 positive cells was 132.6+/-37.4/mm2, 378.6+/-99.1/mm2, 15.0+/-2.8/mm2, 57.1+/-16.9/mm2, 191.8+/-44.8/mm2 and 450.4+/-58.3/mm2, respectively. The number of TUNEL-positive cells was 24.5+/-2.0/mm2, 21.7+/-4.0/mm2, 20.4+/-2.0/mm2, 18.96+/-2.1/mm2, 58.3+/-7.9/mm2, and 106.0+/-9.8/mm2, respectively. These results suggest low doses of radiation paradoxically improved diabetes induced neuronal cell suppression in the hippocampal dentate gyrus of rat.
authors.affiliation: Department of Radiation Oncology, School of Medicine, Kyung Hee University, Seoul, Korea. kangjino@khmc.or.kr
authors.forename: Jin Oh
authors.initials: JO
authors.lastname: Kang
authors.forename: Sang Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: Seong Eon
authors.initials: SE
authors.lastname: Hong
authors.forename: Taeck Hyun
authors.initials: TH
authors.lastname: Lee
authors.forename: Chang Ju
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis; D001973:Bromodeoxyuridine / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000187:drug effects / Q000528:radiation effects*; D003921:Diabetes Mellitus, Experimental / Q000532:radiotherapy; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects; D020287:In Situ Nick-End Labeling; D008297:Male; D009474:Neurons / Q000378:metabolism*; D011878:Radiotherapy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D053148:Caspase 3; D001973:Bromodeoxyuridine