Production of murine monoclonal anti-B.
OBJECTIVE
Monoclonal antibodies against red blood cell antigens used in research and as diagnostics in India are commercially procured from western countries. Indigenously generated potent clones are not available in India. Hence, the objective of the present study was to raise potent murine monoclonal antibodies against A, B and H blood group antigens indigenously and establish a stable clone of anti-B secreting cells.


METHODS
Spleen cells of female BALB/c mice immunized with B group red blood cells were fused in presence of polyethylene glycol (PEG) 1500 with a mouse myeloma cell line Sp 2/0 Ag. 14 in hypoxanthine aminopterine thymidine (HAT) selective medium and incubated at 37 degrees C, 5 per cent CO(2) and 95 per cent humidity for a week.


RESULTS
The culture supernatant of the wells showing anti-B activity, were further subcloned and a clone 2C4D5F10 was generated which showed a good potency, avidity and specificity.


CONCLUSIONS
The anti-B clones thus produced indigenously provided a useful reagent in blood group typing. The unlimited availability unlike polyclonal antisera makes this reagent more cost-effective. It also ensures a regular supply with the similar specificity.
Iyer
Y S
YS
Institute of Immunohematology (ICMR), NMS Building, KEM Hospital Campus, Parel, Mumbai 400 012, India. yegneswar2003@yahoo.com
Vasantha
K
K
Manisha
P
P
Jadhav
S
S
Gupte
S C
SC
Mohanty
D
D
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000915:Antibody Affinity; D002459:Cell Fusion; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007194:India; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents