Management of fluoride induced testicular disorders by calcium and vitamin-E co-administration in the albino rat.
Fluoride contamination of drinking water can disrupt male gametogenesis and steroidogenesis and induce testicular oxidative stress. Treatment of rats with sodium fluoride at the dose of 20 mg/kg/day for 28 days resulted in significant diminution of testicular Delta5,3beta-hydroxysteroid dehydrogenase (HSD) and 17beta-hydroxysteroid dehydrogenase (HSD) activities and low plasma levels of testosterone, follicular stimulating hormone (FSH) and leutinizing hormone (LH). Spermatogenesis inhibited after sodium fluoride treatment has been assessed here by the quantification of different generation of germ cells like spermatogonia A (ASg), preleptotene spermatocyte (PLSc), midpachytene spermatocyte (MPSc) and step 7 spermatid (7Sd) at stage VII of seminiferous epithelial cycle. Furthermore, fluoride treatment was associated with low activities of testicular, prostatic and epididymal catalase (CAT), superoxide dismutase (SOD) and peroxidase along with elevation of malondialdehyde (MDA) and conjugated dienes (CD) in those tissues. Co-administration of calcium and Vitamin-E with fluoride resulted in a significant recovery from testicular disorders and oxidative stress in the testis and male accessory sex organs. The results of this study demonstrate that fluoride exposure, at the dose available in drinking water in contaminated areas, led to inhibition of testicular gametogenesis and steroidogenesis in association with oxidative stress in the testis and male accessory sex organs, which are protected significantly by dietary agents like Vitamin-E and calcium.
Sarkar
S Das
SD
Reproductive Endocrinology and Family Welfare Research Unit, Department of Human Physiology with Community Health,Vidyasagar University, Midnapore, West Bengal, India.
Maiti
R
R
Ghosh
D
D
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D002122:Calcium Chloride / Q000008:administration & dosage / Q000097:blood / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D004359:Drug Therapy, Combination; D004793:Enzyme Reactivators / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000097:blood; D005837:Genitalia, Male / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D007442:Intubation, Intratracheal; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity*; D013090:Spermatocytes / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013093:Spermatogonia / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013733:Testicular Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D013739:Testosterone / Q000097:blood; D014810:Vitamin E / Q000008:administration & dosage / Q000097:blood / Q000627:therapeutic use*; D014815:Vitamins / Q000008:administration & dosage / Q000097:blood / Q000627:therapeutic use
D004793:Enzyme Reactivators; D014815:Vitamins; D014810:Vitamin E; D013739:Testosterone; D008315:Malondialdehyde; D012969:Sodium Fluoride; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; C031954:delta(5)-3 beta-hydroxysteroid dehydrogenase; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D002122:Calcium Chloride