Malaysian mosquitocidal soil bacterium (Bacillus thuringiensis) strains with selective hemolytic and lectin activity against human and rat erythrocytes.
The objective of this study is to determine the role of carbohydrates on the toxic effect of parasporal inclusion proteins isolated from Malaysian mosquitocidal Bacillus thuringiensis (Bt) strains on erythrocytes (human and rat). Dose response analyses on the effect of these parasporal inclusions on human and rat erythrocytes suggest that toxin action is selective depending on bacterial strains and source of erythrocytes. Results from this study suggest Bt toxin is a lectin which recognizes specific plasma membrane glycoconjugate receptor(s) with a terminal residue of either D-mannose (Man), N-acetyl-D-galactosamine (GalNAc), N-acetyl-D-glucosamine (GlcNAc) or even a combination of these monosaccharides.
Nadarajah
V D
VD
Human Biology Section, International Medical University, Kuala Lumpur, Malaysia. vishnadevi_nadarajah@imu.edu.my
Chai
S H
SH
Mohammed
S M
SM
Chan
K K
KK
Kanakeswary
K
K
D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000382:microbiology*; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism*; D008296:Malaysia; D009005:Monosaccharides / Q000378:metabolism; D010572:Pest Control, Biological / Q000379:methods*; D051381:Rats; D012988:Soil Microbiology; D013045:Species Specificity; D013171:Spores, Bacterial / Q000378:metabolism
D001426:Bacterial Proteins; D037102:Lectins; D009005:Monosaccharides