Efficient transformation of Legionella pneumophila by high-voltage electroporation.
A simple and reproducible method has been developed to transform Legionella pneumophila by electroporation. Effects of different conditions, including electric field strength, pulse length, DNA quality and cell density, were evaluated. Using our method, an efficiency of up to 6 x 10(7) transformants/microg DNA was obtained. This optimized transformation procedure should efficiently facilitate gene manipulations in L. pneumophila, such as plasmid transfer, transposon mutagenesis, library transformation for complementation cloning, etc.
Chen
Ding-Qiang
DQ
Department of Biochemistry and State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen Zhongshan University, Guangzhou, China.
Huang
Shao-Song
SS
Lu
Yong-Jun
YJ
D004492:Edetic Acid; D018274:Electroporation / Q000379:methods*; D016952:Legionella pneumophila / Q000235:genetics*; D010957:Plasmids; D014169:Transformation, Bacterial
D004492:Edetic Acid