High-throughput affinity mass spectrometry.
Affinity mass spectrometry (AMS) is a proteomics approach for selectively isolating target protein(s) from complex biological fluids for mass spectrometric analysis. The resulting high-content mass spectrometry (MS) data show the unique MS protein signatures (wild-type, posttranslationally modified, as well as genetically modified forms of the protein target) that are present within a biological sample. Information regarding such protein diversity is normally lost in classical proteomic or immunoassay analyses. This chapter presents a step-by-step description of high-throughput AMS in the population proteomic screening of the human plasma protein cystatin C.
Kiernan
Urban A
UA
Intrinsic Bioprobes Inc., Tempe, AZ, USA.
Nedelkov
Dobrin
D
Niederkofler
Eric E
EE
Tubbs
Kemmons A
KA
Nelson
Randall W
RW
D000595:Amino Acid Sequence; D001331:Automation; D001798:Blood Proteins / Q000737:chemistry; D002138:Calibration; D055316:Cystatin C; D015891:Cystatins / Q000097:blood / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D017354:Point Mutation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D012371:Robotics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001798:Blood Proteins; C525030:CST3 protein, human; D055316:Cystatin C; D015891:Cystatins; D011506:Proteins