title: Survival of Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in blood of cats used for transfusions.
abstract: Blood transfusions are commonly administered to cats; associated risks include the transmission of various infectious diseases including Mycoplasma haemofelis (Mhf) and 'Candidatus Mycoplasma haemominutum' (Mhm). Blood transfusions in citrate-phosphate-dextrose-adenine (CPDA-1) solution are commonly administered immediately or stored for up to 1 month prior to administration. It is unknown whether Mhf or Mhm survive in this solution or temperature. The purpose of this study was to determine if Mhf or Mhm remain viable after storage in CPDA-1 for varying periods of time. The results provide evidence that transmission of hemoplasmas to naïve cats occurs after administration of infected feline blood that has been stored in CPDA-1 solution for 1h (Mhf) and 1 week (Mhm). These findings support the recommendation that cats used as blood donors be screened for Mhf and Mhm infections by polymerase chain reaction (PCR) assay prior to use.
authors.affiliation: College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 300 West Drake Road, Ft. Collins, CO 80523, USA. atgary@colostate.edu
authors.forename: Anthony T
authors.initials: AT
authors.lastname: Gary
authors.forename: Holly L
authors.initials: HL
authors.lastname: Richmond
authors.forename: Séverine
authors.initials: S
authors.lastname: Tasker
authors.forename: Timothy B
authors.initials: TB
authors.lastname: Hackett
authors.forename: Michael R
authors.initials: MR
authors.lastname: Lappin
mesh_terms: D000818:Animals; D001782:Blood Donors; D001793:Blood Preservation / Q000662:veterinary*; D001803:Blood Transfusion / Q000662:veterinary*; D002371:Cat Diseases / Q000382:microbiology* / Q000635:transmission; D002415:Cats; D005260:Female; D008297:Male; D009174:Mycoplasma / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary