title: Oxidative stress response to aerobic exercise: comparison of antioxidant supplements.
abstract: OBJECTIVE
  To compare the effects of two antioxidant formulas on biomarkers of oxidative stress before and after aerobic exercise.
  
  
  METHODS
  Aerobically trained men (N=25) and women (N=23) were assigned to one of three treatments: 400 IU of vitamin E+1 g of vitamin C (V; N=15), a fruit and vegetable juice powder concentrate (FV; N=16), or a placebo (P; N=17). Subjects ran for 30 min at 80% VO(2 max) before, after 2 wk of supplementation, and after a 1-wk washout period. Blood samples were taken before and immediately after exercise and analyzed for protein carbonyls (PC), malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), and vitamins C and E.
  
  
  RESULTS
  The V treatment increased plasma vitamin C and E after 2 wk (P <or= 0.05), with no change in the FV or P. Postexercise PC values were elevated for all treatments after all exercise bouts (P< 0.0001). Both V and FV attenuated the exercise-induced increase in PC after 2 wk of supplementation (V=21%, FV=17%), and after the 1-wk washout (V=13%, FV=6%) compared with P (P<0.05), with no differences between V and FV. MDA was unaffected by exercise and treatment. A treatment main effect for 8-OHdG was noted, with values for V lower than for FV and P (4.5+/-2.5, 5.5+/-2.7, and 6.0+/-2.5 ng.mL, respectively; P=0.0002). No exercise session or time main effect was noted for 8-OHdG, suggesting that the lower mean value for the V treatment group was not a result of the supplementation.
  
  
  CONCLUSIONS
  These data suggest that V and FV supplementation for 2 wk can attenuate the rise in PC after 30 min of aerobic exercise, even after a 1-wk washout, without an impact on plasma MDA or 8-OHdG.
authors.affiliation: Department of Health and Sport Sciences, University of Memphis, Memphis, TN 38512, USA. rbloomer@memphis.edu
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Bloomer
authors.forename: Allan H
authors.initials: AH
authors.lastname: Goldfarb
authors.forename: Michael J
authors.initials: MJ
authors.lastname: McKenzie
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000097:blood / Q000627:therapeutic use; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D005638:Fruit; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D010101:Oxygen Consumption / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology*; D010919:Placebos; D011208:Powders; D050050:Protein Carbonylation; D014675:Vegetables; D014801:Vitamin A / Q000627:therapeutic use; D014810:Vitamin E / Q000097:blood / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D001798:Blood Proteins; D010919:Placebos; D011208:Powders; D014801:Vitamin A; D014810:Vitamin E; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D001205:Ascorbic Acid