Lariatins, antimycobacterial peptides produced by Rhodococcus sp. K01-B0171, have a lasso structure.
Two antimycobacterial agents, lariatins A and B, were isolated from the culture broth of Rhodococcus sp. K01-B0171. Their structures were elucidated by spectral analysis and advanced protein chemical methods to be unique cyclic peptides, which consist of 18 and 20 L-amino acid residues with an internal linkage between the gamma-carboxyl group of Glu8 and the alpha-amino group of Gly1. The three-dimensional structure of lariatin A deduced from NMR data by dynamical simulated annealing method indicates that the tail segment (Trp9-Pro18) passes through the ring segment (Gly1-Glu8) to form a 'lasso' structure.
Iwatsuki
Masato
M
Kitasato Institute, Kitasato University, Minato-ku, Tokyo 108, Japan.
Tomoda
Hiroshi
H
Uchida
Ryuji
R
Gouda
Hiroaki
H
Hirono
Shuichi
S
Omura
Satoshi
S
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018698:Glutamic Acid / Q000737:chemistry; D005998:Glycine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D011392:Proline / Q000737:chemistry; D012240:Rhodococcus / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry
D000900:Anti-Bacterial Agents; D010456:Peptides, Cyclic; C522551:lariatin A; C522552:lariatin B; D018698:Glutamic Acid; D014364:Tryptophan; D011392:Proline; D005998:Glycine