title: Effects of histone deacetylase inhibitors on transcriptional regulation of the hsp70 gene in Drosophila.
abstract: Histone acetyltransferases/deacetylases contribute to the activation or inactivation of transcription by modifying the structure of chromatin. Here we examined the effects of histone deacetylase inhibitors (HDIs), trichostatin A, and sodium butyrate on hsp70 gene transcriptional regulation in Drosophila. The chromatin immunoprecipitation assays revealed that HDI treatments induced the hyperacetylation of histone H3 at the promoter and the transcribing regions of hsp70 gene, increased the accessibility of heat-shock factor to target heat-shock element, and promoted the RNA polymerase II-mediated transcription. Moreover, the quantitative real-time PCR confirmed that the HDI-induced hyperacetylation of histone H3 enhanced both the basal and the inducible expression of hsp70 mRNA level. In addition, the acetylation level of histone H3 at the promoter exhibited a fluctuated change upon the time of heat shock. These experimental data implicated a causal link between histone acetylation and enhanced transcription initiation of hsp70 gene in Drosophila.
authors.affiliation: The Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
authors.forename: Yan Mei
authors.initials: YM
authors.lastname: Zhao
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Zhi Gen
authors.initials: ZG
authors.lastname: Yuan
authors.forename: Guo Ling
authors.initials: GL
authors.lastname: Ren
authors.forename: Xiao Xue
authors.initials: XX
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Bai Qu
authors.initials: BQ
authors.lastname: Huang
mesh_terms: D000107:Acetylation; D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology; D007814:Larva; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D002087:Butyrates; D018840:HSP70 Heat-Shock Proteins; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A; D012319:RNA Polymerase II; D006655:Histone Deacetylases