title: Tissue distribution of cefquinome after intramammary and "systemic" administration in the isolated perfused bovine udder.
abstract: Mammary glands taken at slaughter from healthy lactating cows were perfused in vitro with warmed and gassed Tyrode solution. Cefquinome (88.8mg cefquinome sulphate per 8mL) was administered by the intramammary route to all quarters and/or "systemically" via the perfusion fluid at concentrations similar to those measured in plasma following intramuscular administration of 1mg cefquinome per kg body weight. Samples of the perfusate were taken over a 6-h period and from the regional lymph nodes after 6h. Using a scalpel, sections of glandular tissue - at different distances from and vertical to the teat right up to the udder base - were gathered from four quarters each per route of administration at 2, 4 and 6h. The cefquinome content of the tissue samples was analysed by high performance liquid chromatography with diode array detection and of the perfusate samples by bioassay. After intramammary administration, the concentration of cefquinome in the glandular tissue decreased exponentially with increasing distance from the teat. The addition of cefquinome to the perfusion fluid produced a mean concentration of 0.2-0.5microg/g at all glandular tissue sites. Combined intramammary and systemic treatment ensured that concentrations exceeded the MIC(90) values of the most common mastitis pathogens in all areas of the udder by 2h post-administration. There was considerable variability in the tissue concentrations of cefquinome, particularly after intramammary administration. These results suggest that for the treatment of acute mastitis a combination of both intramammary and systemic administration is likely to be advantageous in order to rapidly produce maximum cefquinome concentrations in all regions of the udder.
authors.affiliation: Intervet Innovation GmbH, Zur Propstei, D-55270 Schwabenheim, Germany. andreas.ehinger@intervet.com
authors.forename: A M
authors.initials: AM
authors.lastname: Ehinger
authors.forename: H
authors.initials: H
authors.lastname: Schmidt
authors.forename: M
authors.initials: M
authors.lastname: Kietzmann
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002417:Cattle; D002511:Cephalosporins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D005260:Female; D007263:Infusions, Parenteral / Q000379:methods / Q000662:veterinary; D007273:Injections, Intramuscular / Q000662:veterinary; D008198:Lymph Nodes; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000188:drug therapy*; D010477:Perfusion / Q000662:veterinary; D014018:Tissue Distribution
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; C068212:cefquinome