title: Fluorescence resonance energy transfer in the studies of guanine quadruplexes.
abstract: A guanine (G)-quadruplex DNA motif has recently emerged as a biologically important structure that is believed to interfere with telomere maintenance by telomerase. G-quadruplexes exhibit four-stranded structures containing one or more nucleic acid strands with central channel able to accommodate metal cations. Coordination of certain metal cations stabilizes G-quadruplex as with some promising small organic molecules that promote the formation and/or stabilization of G-quadruplex. Among many techniques employed to explore properties of G-quadruplexes, the fluorescence resonance energy transfer (FRET) technique has been recognized as a powerful tool to study G-quadruplex formation. This review summarizes the current developments in the uses of FRET technique for the fundamental structural investigations and its practical applications. Applications include FRET-based selection of efficient quadruplex-binding ligands, design of a nanomolecular machine, and a molecular aptamer beacon for protein recognition. We also describe a technique for detection of potassium ions in aqueous solution with the use of quadruplex-based sensor (potassium-sensing oligonucleotide).
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland.
authors.forename: Bernard
authors.initials: B
authors.lastname: Juskowiak
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry* / Q000378:metabolism; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D011068:Poly G / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D011068:Poly G; D006147:Guanine; D004247:DNA