title: [Genotype of wild-type measles viruses circulating in Beijing in 2003].
abstract: OBJECTIVE
  To investigate genotype of wild-type measles viruses circulated in Beijing in 2003.
  
  
  METHODS
  Throat swabs specimens were collected from patients seen during an outbreak of measles and from clinically suspected sporadic measles patients in 2003. Vero/SLAM cell lines recommended by WHO were used to isolate measles virus. Four hundreds and fifty nucleotides of COOH-terminal of nucleoprotein (N) genes were amplified by using PR-PCR. The amplified products were sequenced and the sequences were compared with references viruses from GeneBank.
  
  
  RESULTS
  Eight strains of measles viruses were isolated from throat swabs of patients who came from seven districts and counties of Beijing. Sequence analysis of the 450 nucleotides of COOH-terminal of nucleoprotein (N) genes indicated that these 8 strains belonged to H1a genotype. The average genetic distances of these 8 strains to H1a genotype, Chin9322, H1b genotype, Chin9475 and H1c genotype, Chin9427, were 0.004 - 0.011, 0.026 - 0.031 and 0.015 - 0.022, respectively. The average genetic distances of these 8 strains to H1a genotype, Anhui 01 - 1/Anhui 02 - 2, were 0.000 - 0.009 (0 - 5 nucleotide variation).
  
  
  CONCLUSIONS
  Major genotypes of wild-type measles viruses circulated in Beijing in 2003 were H1a genotype. The genotypes H1c, H1b and H2 may have disappeared in Beijing.
authors.affiliation: Virology Laboratory, Beijing Children's Hospital Affiliated to Capital University of Medical Sciences, Beijing 100045, China.
authors.forename: Zheng-de
authors.initials: ZD
authors.lastname: Xie
authors.forename: Yi-xin
authors.initials: YX
authors.lastname: Ji
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ya-yi
authors.initials: YY
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Yan
authors.forename: Ying
authors.initials: Y
authors.lastname: Mai
authors.forename: Kun-ling
authors.initials: KL
authors.lastname: Shen
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Xu
mesh_terms: D002648:Child; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D005838:Genotype; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D008457:Measles / Q000453:epidemiology / Q000276:immunology / Q000821:virology*; D008459:Measles virus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics*; D012367:RNA, Viral / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D007075:Immunoglobulin M; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D012367:RNA, Viral; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus