Molecular cloning of nuclear receptor coactivator-1 gene in pig.
Nuclear receptor coactivator-1 (NCOA-1) as a member of Steroid receptor coactivator (SRC) family can interact with some DNA-bound nuclear receptors and enhance their transcriptional activation function, which is physiologically and pathologically important for animals and human. Much work has been done on the gene in human and mouse, but few in pig. Here we cloned and sequenced full length cDNA of NCOA-1 gene in pig. The putative protein includes 1440 amino acids. Sequences alignment showed that pig NCOA-1 gene cDNA sequence has identity of 93.73% with human and mouse, and putative pig NCOA-1 protein has identity of 94.99% with human, mouse and rat. The motifs, LXXLL, which have been demonstrated to be important for NCOA-1 mediating ligand-dependent and direct interaction with nuclear receptor, were conserved in pig.
Yu
Dandan
D
Department of Animal Science, Agriculture and Biology School, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Mneg
He
H
Bai
Chunyan
C
Zhao
W
W
Wang
Q S
QS
Pan
Yuchun
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D051548:Histone Acetyltransferases; D008969:Molecular Sequence Data; D056920:Nuclear Receptor Coactivator 1; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D014157:Transcription Factors; D051548:Histone Acetyltransferases; C538859:NCOA1 protein, human; C538861:Ncoa1 protein, mouse; D056920:Nuclear Receptor Coactivator 1