A new Minos vector for eye-specific expression of white+ marker in Ceratitis capitata and in distantly related dipteran species.
The genetic transformation of insects by transposable elements is based on the use of selectable genetic markers required to identify transgenic individuals. Conserved regulatory sequences can be used to develop single constructs capable of adequate expression of a marker, across a range of different species. We present evidence that the Drosophila GBS regulatory element (Glass-binding site), derived from the Rh1 rhodopsin gene, is able to drive in vivo eye-specific expression of a Ccwhite+ transgene in the Mediterranean fruitfly Ceratitis capitata. The Ceratitis lineage diverged from that of Drosophila approximately 120 Myr ago. As the GBS regulatory sequence seems to be partially conserved in the more distantly related dipteran species Anopheles gambiae (250 Myr), we propose that the GBS may be widely useful for driving eye-specific expression in a wide range of dipteran species.
Salvemini
M
M
Department of Biological Sciences, Section of Genetics and Molecular Biology, University Federico II, Naples, Italy.
Mauro
U
U
Velaeti
S
S
Polito
C
C
Saccone
G
G
D000818:Animals; D000852:Anopheles / Q000235:genetics; D033622:Ceratitis capitata / Q000235:genetics / Q000378:metabolism*; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000378:metabolism; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins / Q000378:metabolism; D005123:Eye / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D017344:Genes, Insect; D005822:Genetic Vectors; D008297:Male; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism*; D010858:Pigmentation; D010957:Plasmids; D050436:Regulatory Elements, Transcriptional; D012243:Rhodopsin / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic; D019076:Transgenes; D019895:Transposases
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C087289:gl protein, Drosophila; D012243:Rhodopsin; C473499:Minos transposase; D019895:Transposases