Assessment of oxidative damage induced by acute doses of morphine sulfate in postnatal and adult rat brain.
The aim of the present study is to evaluate the oxidative damage in rats of different ages. Weaned rats of 25 g and adults of 300 g were used in groups of 6, a single i.p. dose of morphine sulfate of 3, 6 or 12 mg/kg was administered. All animals were sacrificed to measure GSH and 5-HT levels in brain by liquid chromatography, as well as Na(+), K(+)-ATPase and total ATPase enzymatic activity. 5-HT levels decreased significantly (p < 0.05) in adult animals that received 3 and 6 mg morphine. Na(+), K(+)-ATPase activity increased significantly (p < 0.05) in all groups of weaned animals. In adult animals, Na(+), K(+)-ATPase and total ATPase partially diminished. GSH levels diminished significantly (p < 0.05) both in weaned and in adult groups. The results indicate age-induced changes in cellular regulation and biochemical responses to oxidative stress induced by morphine.
Guzmán
David Calderón
DC
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), Mexico DF, Mexico.
Vázquez
Ivonne Espítia
IE
Brizuela
Norma Osnaya
NO
Alvarez
Raquel García
RG
Mejía
Gerardo Barragán
GB
García
Ernestina Hernández
EH
Santamaría
Daniel
D
de Apreza
Mario la Rosa
Ml
Olguín
Hugo Juárez
HJ
D000367:Age Factors; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009294:Narcotics / Q000494:pharmacology*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D009294:Narcotics; D012701:Serotonin; D009020:Morphine; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione