Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes.
Bidens pilosa (B. pilosa) is well known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of both the ethanol (EtOH) and ethylacetate/ethanol (EA/EtOH) extracts from the whole B. pilosa plant, to protect normal human erythrocytes against oxidative damage in vitro. It was determined that the oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by the aqueous peroxyl radical [2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)] were suppressed by both EtOH (50-150 microg/ml) and EA/EtOH (25-75 microg/ml) extracts of B. pilosa in concentration- and time-dependent manners. B. pilosa extracts also prevented the decline of superoxide dismutase (SOD) activity and the depletion of cytosolic glutathione (GSH) and ATP in erythrocytes. These results imply that B. pilosa may have protective antioxidant properties.
Yang
Hsin-Ling
HL
Institute of Nutrition, China Medical University, Taichung, Taiwan.
Chen
Ssu-Ching
SC
Chang
Nai-Wen
NW
Chang
Jia-Ming
JM
Lee
Mei-Ling
ML
Tsai
Pei-Chuan
PC
Fu
Han-Hsuan
HH
Kao
Wei-Wan
WW
Chiang
Hsiao-Chi
HC
Wang
Hsuan-Hui
HH
Hseu
You-Cheng
YC
D000085:Acetates / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology*; D031176:Bidens / Q000737:chemistry; D002478:Cells, Cultured; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism
D000085:Acetates; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D008565:Membrane Proteins; D000431:Ethanol; C007650:ethyl acetate; D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase; D005978:Glutathione