Messenger RNA expression of RANTES in the urinary sediment of patients with lupus nephritis.
BACKGROUND
Lupus nephritis is characterized by intra-renal inflammation. Patients with systemic lupus erythematosus (SLE) showed abnormal T-cell expression of RANTES (regulated upon activation, normal T cell expressed) and its level in their serum. The authors studied the mRNA expression of RANTES in the urinary sediment of lupus patients.


METHODS
The authors studied 88 lupus patients, who were classified into active, remission and non-renal SLE groups according to the disease activity, 29 non-SLE and 10 healthy controls. Lupus activity was assessed by the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). Urinary mRNA expression of RANTES was studied by real-time quantitative polymerase chain reaction.


RESULTS
The expression of RANTES in urinary sediment was significantly elevated in active group (P < 0.001). Expression level of RANTES correlated with the SLEDAI score (r = 0.57; P < 0.001) and renal score in SLEDAI (r = 0.60; P < 0.001). In addition, urinary expression of RANTES had significant correlation with degree of proteinuria, serum creatinine, albumin and estimated glomerular filtration rate.


CONCLUSIONS
The authors conclude that the mRNA expression of RANTES was elevated in the urinary sediment of patients with active lupus nephritis. Measurement of urinary mRNA expression may be a novel non-invasive method for the assessment of lupus disease activity and the severity of renal involvement.
Chan
Rebecca Wing-Yan
RW
Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China.
Lai
Fernand Mac-Moune
FM
Li
Edmund Kwok-Ming
EK
Tam
Lai-Shan
LS
Chow
Kai-Ming
KM
Li
Philip Kam-Tao
PK
Szeto
Cheuk-Chun
CC
D000328:Adult; D018946:Chemokine CCL5 / Q000235:genetics* / Q000652:urine*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008181:Lupus Nephritis / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology / Q000652:urine*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D012307:Risk Factors; D012680:Sensitivity and Specificity
D018946:Chemokine CCL5; D012333:RNA, Messenger