title: AB-type lectin (toxin/agglutinin) from mistletoe: differences in affinity of the two galactoside-binding Trp/Tyr-sites and regulation of their functionality by monomer/dimer equilibrium.
abstract: Viscumin of mistletoe (Viscum album L.) has a concentration-dependent activity profile unique to plant AB-toxins. It starts with lectin-dependent mitogenicity and then covers toxicity and cell agglutination, associated with shifts in the monomer/dimer equilibrium. Each lectin subunit harbors two sections for ligand contact. In the dimer, the B-chain sites in subdomain 2 gamma (designated as the Tyr-sites) appear fully accessible, whereas Trp-sites in subdomain 1 alpha are close to the dimer interface. It is unclear whether both types of sites operate similarly in binding glycoligands in solution. By systematically covering a broad range of lactose/lectin ratio in isothermal titration calorimetry, we obtained evidence for two sites showing dissimilar binding affinity. Intriguingly, the site with higher affinity was only partially occupied. To assign the observed properties to the Trp/Tyr-sites, we next performed chemically induced dynamic nuclear polarization measurements of Trp and Tyr accessibility. A Tyr signal, but not distinct Trp peaks, was recorded when testing the dimer. Lactose-quenchable Trp peaks became visible on the destabilization of the dimer by citraconylation, intimating Trp involvement in ligand contact in the monomer. Fittingly, Tyr acetylation but not mild Trp oxidation reduced the dimer hemagglutination activity and the extent of binding to asialofetuin-Sepharose 4B. Altogether, the results attribute lectin activity in the dimer primarily to Tyr-sites. Full access to Trp-sites is gained on dimer dissociation. Thus, the monomer/dimer equilibrium of viscumin regulates the operativity of these sites. Their structural divergence affords the possibility for differences in ligand selection when comparing monomers (Tyr- and Trp-sites) with dimers (primarily Tyr-sites).
authors.affiliation: Instituto de Química Física Rocasolano, CSIC, Serrano, Madrid, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Jiménez
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.forename: Hans-C
authors.initials: HC
authors.lastname: Siebert
authors.forename: Hans-J
authors.initials: HJ
authors.lastname: Gabius
authors.forename: Dolores
authors.initials: D
authors.lastname: Solís
mesh_terms: D001212:Asialoglycoproteins / Q000493:pharmacokinetics; D002850:Chromatography, Gel / Q000379:methods; D019281:Dimerization; D060748:Fetuins; D005697:Galactosides / Q000378:metabolism*; D006384:Hemagglutination / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007785:Lactose / Q000378:metabolism; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism*; D008859:Microscopy, Polarization / Q000379:methods; D014784:Mistletoe / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism; D028321:Plant Preparations / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D011485:Protein Binding; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012685:Sepharose / Q000493:pharmacokinetics; D013379:Substrate Specificity; D014118:Toxins, Biological / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000493:pharmacokinetics
chemical_list: D001212:Asialoglycoproteins; D060748:Fetuins; D005697:Galactosides; D037102:Lectins; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; D000509:alpha-Fetoproteins; C017029:asialofetuin; C488862:mistletoe lectin I; C488863:mistletoe lectin II; D014443:Tyrosine; D014364:Tryptophan; D012685:Sepharose; D054788:Ribosome Inactivating Proteins; D007785:Lactose