Immunoproteomic analysis of the murine antibody response to successful and failed immunization with live anti-Francisella vaccines.
Francisella tularensis subspecies tularensis is one of the most virulent of bacterial pathogens for humans. Protective immunity against the pathogen can be induced in humans and some, but not all, mouse strains by vaccination with live, but not killed, vaccines. In mice, this protection is mediated predominantly by CD4+ and CD8+ T cells. This is thought to be the case too for humans. Nevertheless, it is possible that successful vaccination elicits antigen-specific antibodies that can serve as correlates of protection. To test this hypothesis we examined the repertoire of antibodies induced following successful immunization of BALB/c and CH3/HeN mice versus unsuccessful vaccination of C57BL/6 and DBA\2 mice with F. tularensis Live Vaccine Strain or following unsuccessful vaccination of BALB/c mice with highly related subspecies, F. novicida. The results showed that successful vaccination elicited antibodies to at least six proteins that were not recognized by antisera from vaccinated but unprotected mice.
Twine
Susan M
SM
Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ont., Canada K1A 0R6. susan.twine@nrc-cnrc.gc.ca
Petit
Mireille D
MD
Shen
Hua
H
Mykytczuk
Nadia C S
NC
Kelly
John F
JF
Conlan
J Wayne
JW
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology* / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005604:Francisella tularensis / Q000276:immunology*; D051379:Mice; D040901:Proteomics; D014611:Vaccination
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines