Effect of synthetic cell-binding peptide on the healing of cortical segmental bone defects.
OBJECTIVE
To determine the effect of inorganic bone matric/Pepgen P-15 (ABM/P-15) on the healing of a critical sized segmental defect in a rat radius using a radiological and histological grading system.


METHODS
We carried out this study at the Research Laboratories, Gazi University School of Medicine in 2004. Critical sized segmental defects were created in the radius of 36 Wistar rats. Thirteen defects were filled with ABM/P-15 Flow (gel form), 12 defects were filled with ABM/P-15, and 11 defects were used as a control group. The rats were sacrificed at the tenth week, and healing of the defects was evaluated radiographically and histologically.


RESULTS
The usage of ABM/P-15 and ABM/P-15 Flow were demonstrated to improve healing of segmental bone defects compared with the control group. Statistical evaluation showed that there were significant differences between control sites, and the sites treated with P-15 and P-15 Flow (p=0.011). The highest radiological and histological grades were achieved by P-15.


CONCLUSIONS
Segmental cortical bone defects may be treated with ABM/P-15 instead of bone allografts, and autografts. According to the radiological and histological parameters measured in this study, the implantation of ABM/P-15 resulted in optimum healing of the segmental cortical bone defects. Pepgen P-15 has a positive effect on bone healing, without any immunogenic features and disease transmission risk. Therefore, ABM/P-15 can also be used for orthopedic surgery.
Cakmak
Gokhan
G
Department of Orthopedics and Traumatology, Gazi University School of Medicine, Ankara, Turkey. gcakmak@ttnet.net.tr
Bolukbasi
Selcuk
S
Simsek
Aykin
A
Erdem
Ozlem
O
Yilmaz
Guldal
G
Senkoylu
Alpaslan
A
D000818:Animals; D001857:Bone Matrix / Q000000981:diagnostic imaging / Q000473:pathology; D018786:Bone Substitutes / Q000494:pharmacology*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000293:injuries* / Q000473:pathology; D002146:Bony Callus / Q000000981:diagnostic imaging; D003094:Collagen / Q000494:pharmacology*; D004338:Drug Combinations; D010006:Osteoblasts / Q000000981:diagnostic imaging / Q000473:pathology; D010446:Peptide Fragments / Q000494:pharmacology*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
D018786:Bone Substitutes; D004338:Drug Combinations; C403137:PepGen P-15; D010446:Peptide Fragments; C407062:cell-binding peptide P-15; D003094:Collagen