title: Requirements for effective IgE cross-linking on mast cells and basophils.
abstract: This article reviews the characteristics of high affinity IgE receptors (FcepsilonRI) and their role in the response to allergenic proteins. The requirements for successful cross-linking of FcepsilonRI on basophils and mast cells and subsequent degranulation by allergenic proteins will be explained in detail. Methods for in vitro analysis of allergen-induced mast cell and basophil degranulation will be described and issues/problems in applying these methods will be discussed. Finally, implications for manipulation of protein allergens will be discussed.
authors.affiliation: Department of Dermatology/Allergology, University Medical Center Utrecht, Utrecht, The Netherlands. e.f.knol@umcutrecht.nl
authors.forename: Edward F
authors.initials: EF
authors.lastname: Knol
mesh_terms: D000485:Allergens / Q000276:immunology; D001491:Basophils / Q000276:immunology*; D015550:Cell Degranulation; D003432:Cross-Linking Reagents; D007073:Immunoglobulin E / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D008407:Mast Cells / Q000276:immunology*; D011506:Proteins / Q000276:immunology; D017455:Receptors, IgE / Q000737:chemistry / Q000276:immunology / Q000378:metabolism
chemical_list: D000485:Allergens; D003432:Cross-Linking Reagents; D007074:Immunoglobulin G; D011506:Proteins; D017455:Receptors, IgE; D007073:Immunoglobulin E