MR three-dimensional molecular imaging of intramural biomarkers with targeted nanoparticles.
In this study, porcine carotid arteries were subjected to balloon overstretch injury followed by local delivery of paramagnetic nanoparticles targeted to alphavbeta3-integrin expressed by smooth muscle cells or collagen III within the extracellular matrix. Carotid T1-weighted angiography and vascular imaging was performed at 1.5T. While MR angiograms were indistinguishable between control and targeted vessel segments, alphavbeta3-integrin-and collagen Ill-targeted nanoparticles spatially delineated patterns and volumes of stretch injury. In conclusion, MR molecular imaging with alphavbeta3-integrin or collagen Ill-targeted nanoparticles enables the non-invasive, three-dimensional characterization of arterial pathology unanticipated from routine angiography.
Cyrus
Tillmann
T
Division of Cardiology, Washington University School of Medicine, Saint Louis, Missouri 63110, USA. tcyrus@cmrl.wustl.edu
Abendschein
Dana R
DR
Caruthers
Shelton D
SD
Harris
Thomas D
TD
Glattauer
Veronica
V
Werkmeister
Jerome A
JA
Ramshaw
John A M
JA
Wickline
Samuel A
SA
Lanza
Gregory M
GM
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002339:Carotid Arteries / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D004655:Emulsions; D005109:Extracellular Matrix / Q000378:metabolism*; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D018810:Magnetic Resonance Angiography; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism*; D049329:Nanostructures; D013552:Swine; D014654:Vascular Patency
D015415:Biomarkers; D024061:Collagen Type III; D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D039302:Integrin alphaVbeta3; C003072:perflubron