Determination of gatifloxacin and ornidazole in tablet dosage forms by high-performance thin-layer chromatography.
A simple and sensitive high-performance thin-layer chromatography (HPTLC) method has been developed for the quantitative estimation of gatifloxacin and ornidazole in its combined dosage forms. Gatifloxacin and ornidazole were chromatographed on silica Gel 60 F(254) TLC plate using n-butanol:methanol:ammonia (6 M) (8:1:1.5 v/v) as the mobile phase and scanned at 302 nm using a Camag TLC Scanner 3. The R(f) value of gatifloxacin and ornidazole was found to be 0.21 +/- 0.02 and 0.76 +/- 0.04, respectively. The linearity of gatifloxacin and ornidazole were in the range of 100 - 500 ng/spot and 250 - 1250 ng/spot, respectively. The limit of detection was found to be 40 ng/spot for gatifloxacin and 100 ng/spot for ornidazole. The proposed method was applied for the determination of gatifloxacin and ornidazole in combined dosage forms.
Suhagia
Bhanubhai N
BN
Department of Quality Assurance, L. M. College of Pharmacy.
Shah
Shailesh A
SA
Rathod
Ishwarsinh S
IS
Patel
Haresh M
HM
Shah
Dinesh R
DR
Marolia
Bhavin P
BP
D000890:Anti-Infective Agents / Q000032:analysis* / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D024841:Fluoroquinolones / Q000032:analysis* / Q000592:standards; D000077734:Gatifloxacin; D009950:Ornidazole / Q000032:analysis* / Q000592:standards; D012680:Sensitivity and Specificity; D013607:Tablets
D000890:Anti-Infective Agents; D004338:Drug Combinations; D024841:Fluoroquinolones; D013607:Tablets; D009950:Ornidazole; D000077734:Gatifloxacin