title: The design of silk fiber composition in moths has been conserved for more than 150 million years.
abstract: The silk of caterpillars is secreted in the labial glands, stored as a gel in their lumen, and converted into a solid filament during spinning. Heavy chain fibroin (H-fibroin), light chain fibroin (L-fibroin), and P25 protein constitute the filament core in a few species that have been analyzed. Identification of these proteins in Yponomeuta evonymella, a moth from a family which diverged from the rest of Lepidoptera about 150 million years ago, reveals that the mode of filament construction is highly conserved. It is proposed that association of the three proteins is suited for long storage of hydrated silk dope and its rapid conversion to filament. Interactions underlying these processes depend on conserved spacing of critical amino acid residues that are dispersed through the L-fibroin and P25 and assembled in the short ends of the H-fibroin molecule. Strength, elasticity, and other physical properties of the filament are determined by simple amino acid motifs arranged in repetitive modules that build up most of the H-fibroin. H-Fibroin synergy with L-fibroin and P25 does not interfere with motif diversification by which the filament acquires new properties. Several types of motifs in complex repeats occur in the silks used for larval cobwebs and pupal cocoons. Restriction of silk use to cocoon construction in some lepidopteran families has been accompanied by simplification of H-fibroin repeats. An extreme deviation of the silk structure occurs in the Saturniidae silkmoths, which possess modified H-fibroin and lack L-fibroin and P25.
authors.affiliation: Institute of Entomology and Faculty of Biological Sciences, Academy of Sciences, University of South Bohemia, Branisovská 31, 370 05, Ceské Budejovice, Czech Republic.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Yonemura
authors.forename: Frantisek
authors.initials: F
authors.lastname: Sehnal
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000302:isolation & purification; D019143:Evolution, Molecular; D005349:Fibroins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D047011:Silk / Q000096:biosynthesis*; D013045:Species Specificity; D040681:Structural Homology, Protein; D013329:Structure-Activity Relationship; D013995:Time
chemical_list: D018076:DNA, Complementary; D006023:Glycoproteins; D019476:Insect Proteins; C432480:L-chain, fibroin protein, insect; C111320:P25 protein, Galleria mellonella; D047011:Silk; D005349:Fibroins