Novel ELISA system for detection of N-ERC/mesothelin in the sera of mesothelioma patients.
We have developed a novel enzyme-linked immunosorbent assay (ELISA) system for the detection of N-ERC/mesothelin in the serum of mesothelioma patients and have begun to examine its clinical usefulness. N-ERC/mesothelin is a 31-kDa protein that forms the N-terminal fragment of the full-length 71-kDa ERC/mesothelin protein, and is physiologically secreted into the blood of mesothelioma patients where it can be detected using our sandwich ELISA containing two antibodies (rabbit polyclonal anti-ERC/mesothelin antibody-282 and mouse monoclonal antibody 7E7). Our ELISA system has thus far detected much higher serum levels of N-ERC/mesothelin in mesothelioma patients than in healthy controls or patients with other lung or pleural diseases. In conclusion, N-ERC/mesothelin is a promising candidate tumor marker for mesothelioma.
Shiomi
Kazu
K
Department of General Thoracic Surgery, Respiratory Medicine, Juntendo University, School of Medicine, 2-1-1 Hongo, Tokyo 113-8421, Japan.
Miyamoto
Hideaki
H
Segawa
Tatsuya
T
Hagiwara
Yoshiaki
Y
Ota
Akinobu
A
Maeda
Masahiro
M
Takahashi
Kazuhisa
K
Masuda
Kimihiko
K
Sakao
Yukinori
Y
Hino
Okio
O
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000097:blood*; D015153:Blotting, Western; D019556:COS Cells; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D058851:GPI-Linked Proteins; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000097:blood*; D000090204:Mesothelin; D008654:Mesothelioma / Q000097:blood* / Q000175:diagnosis*; D051379:Mice; D011817:Rabbits
D000906:Antibodies; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C000715528:Msln protein, mouse; D000090204:Mesothelin