title: Secreted proteins and genes in fetal and neonatal pig adipose tissue and stromal-vascular cells.
abstract: Although microarray and proteomic studies have indicated the expression of unique and unexpected genes and their products in human and rodent adipose tissue, similar studies of meat animal adipose tissue have not been reported. Thus, total RNA was isolated from stromal-vascular (S-V) cell cultures (n = 4; 2 arrays; 2 cultures/array) from 90-d (79% of gestation) fetuses and adipose tissue from 105-d (92% of gestation) fetuses (n = 2) and neonatal (5-d-old) pigs (n = 2). Duplicate adipose tissue microarrays (n = 4) represented RNA samples from a pig and a fetus. Dye-labeled cDNA probes were hybridized to custom microarrays (70-mer oligonucleotides) representing more than 600 pig genes involved in growth and reproduction. Microarray studies showed significant expression of 40 genes encoding for known adipose tissue secreted proteins in fetal S-V cell cultures and adipose tissue. Expression of 10 genes encoding secreted proteins not known to be expressed by adipose tissue was also observed in neonatal adipose tissue and fetal S-V cell cultures. Additionally, the agouti gene was detected by reverse transcription-PCR in pig S-V cultures and adipose tissue. Proteomic analysis of adipose tissue and fetal and young pig S-V cell culture-conditioned media identified multiple secreted proteins including heparin-like epidermal growth factor-like growth factor and several apolipoproteins. Another adipose tissue secreted protein, plasminogen activator inhibitor-1, was identified by ELISA in S-V cell culture media. A group of 20 adipose tissue secreted proteins were detected or identified using the gene microarray and the proteomic and protein assay approaches including apolipoprotein-A1, apolipoprotein-E, relaxin, brain-derived neurotrophic factor, and IGF binding protein-5. These studies demonstrate, for the first time, the expression of several major secreted proteins in pig adipose tissue that may influence local and central metabolism and growth.
authors.affiliation: USDA-ARS, Russell Agricultural Research Center, Athens, GA 30604, USA. ghausman@saa.ars.usda.gov
authors.forename: G J
authors.initials: GJ
authors.lastname: Hausman
authors.forename: S P
authors.initials: SP
authors.lastname: Poulos
authors.forename: R L
authors.initials: RL
authors.lastname: Richardson
authors.forename: C R
authors.initials: CR
authors.lastname: Barb
authors.forename: T
authors.initials: T
authors.lastname: Andacht
authors.forename: H C
authors.initials: HC
authors.lastname: Kirk
authors.forename: R L
authors.initials: RL
authors.lastname: Mynatt
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D020411:Oligonucleotide Array Sequence Analysis; D017154:Stromal Cells / Q000378:metabolism*; D013552:Swine / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned