title: MR three-dimensional molecular imaging of intramural biomarkers with targeted nanoparticles.
abstract: In this study, porcine carotid arteries were subjected to balloon overstretch injury followed by local delivery of paramagnetic nanoparticles targeted to alphavbeta3-integrin expressed by smooth muscle cells or collagen III within the extracellular matrix. Carotid T1-weighted angiography and vascular imaging was performed at 1.5T. While MR angiograms were indistinguishable between control and targeted vessel segments, alphavbeta3-integrin-and collagen Ill-targeted nanoparticles spatially delineated patterns and volumes of stretch injury. In conclusion, MR molecular imaging with alphavbeta3-integrin or collagen Ill-targeted nanoparticles enables the non-invasive, three-dimensional characterization of arterial pathology unanticipated from routine angiography.
authors.affiliation: Division of Cardiology, Washington University School of Medicine, Saint Louis, Missouri 63110, USA. tcyrus@cmrl.wustl.edu
authors.forename: Tillmann
authors.initials: T
authors.lastname: Cyrus
authors.forename: Dana R
authors.initials: DR
authors.lastname: Abendschein
authors.forename: Shelton D
authors.initials: SD
authors.lastname: Caruthers
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Harris
authors.forename: Veronica
authors.initials: V
authors.lastname: Glattauer
authors.forename: Jerome A
authors.initials: JA
authors.lastname: Werkmeister
authors.forename: John A M
authors.initials: JA
authors.lastname: Ramshaw
authors.forename: Samuel A
authors.initials: SA
authors.lastname: Wickline
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Lanza
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002339:Carotid Arteries / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D004655:Emulsions; D005109:Extracellular Matrix / Q000378:metabolism*; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D018810:Magnetic Resonance Angiography; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism*; D049329:Nanostructures; D013552:Swine; D014654:Vascular Patency
chemical_list: D015415:Biomarkers; D024061:Collagen Type III; D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D039302:Integrin alphaVbeta3; C003072:perflubron