title: Effect of fluid resuscitation on ryanodine receptor in macaques with endotoxic shock.
abstract: OBJECTIVE
  Our recent study demonstrated that sodium bicarbonate improved cardiac function in macaque models with early-phase endotoxic shock. In the present study, we investigated further the ryanodine receptor/calcium release-channel (RyR) and calcium pump after fluid resuscitation of macaques with early-phase endotoxic shock.
  
  
  METHODS
  Twenty-four anaesthetised macaques were assigned to four groups. Nineteen animals were given an intravenous dose of 2.8 mgkg(-1) lipopolysaccharide (LPS). Sixty minutes after the LPS challenge, the animals were given (i) 5 mLkg(-1) normal saline (Ns group, n = 6), (ii) 5 mLkg(-1) of 5% sodium bicarbonate (Sb group, n = 6) or (iii) 5 mLkg(-1) of 3.5% hypertonic sodium chloride (Hs group, n = 7). The control group (Co group, n = 5) received 1 mLkg(-1) normal saline and then with 5 mLkg(-1) normal saline 60 min later.
  
  
  RESULTS
  Endotoxin produced a reduction of the density of RyR but did not alter the affinity of RyR. Compared with normal saline, sodium bicarbonate or hypertonic saline induced a restoration of density of RyR but did not influence the affinity of RyR and the calcium pump.
  
  
  CONCLUSIONS
  Up-regulation of RyR performance in myocardium following administration of sodium bicarbonate contributes to the improvement of cardiac function in macaques in the early phase of endotoxic shock.
authors.affiliation: The Second Hospital of Nanjing, Medical College, Southeast University, 1-1 Zhong-fu Road, Nanjing, Jiangsu 210003, PR China. yinguoq@jlonline.com
authors.forename: Guo Qing
authors.initials: GQ
authors.lastname: Yin
authors.forename: Xiao Hui
authors.initials: XH
authors.lastname: Ji
authors.forename: Fei Rong
authors.initials: FR
authors.lastname: Gu
authors.forename: Cheng Ping
authors.initials: CP
authors.lastname: Lu
authors.forename: Zhi Xun
authors.initials: ZX
authors.lastname: Fang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Shen
authors.forename: Jia Qi
authors.initials: JQ
authors.lastname: Tang
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000187:drug effects* / Q000378:metabolism; D004195:Disease Models, Animal; D004926:Escherichia coli; D005260:Female; D005440:Fluid Therapy; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008251:Macaca; D008297:Male; D009206:Myocardium / Q000378:metabolism; D012151:Resuscitation / Q000379:methods*; D019837:Ryanodine Receptor Calcium Release Channel / Q000187:drug effects* / Q000378:metabolism; D012462:Saline Solution, Hypertonic / Q000494:pharmacology* / Q000627:therapeutic use; D012772:Shock, Septic / Q000209:etiology / Q000378:metabolism* / Q000628:therapy; D017693:Sodium Bicarbonate / Q000494:pharmacology* / Q000627:therapeutic use; D015854:Up-Regulation
chemical_list: D008070:Lipopolysaccharides; D019837:Ryanodine Receptor Calcium Release Channel; D012462:Saline Solution, Hypertonic; D017693:Sodium Bicarbonate; D000252:Calcium-Transporting ATPases