A novel method for construction of gene fragment library to searching epitopes.
Identification of the epitope sequence or the functional domain of proteins is a laborious process but a necessary one for biochemical and immunological research. To achieve intensive and effective screening of these functional peptides in various molecules, we established a novel screening method using a phage library system that displays various lengths and parts of peptides derived from target protein. Applying this library for epitope mapping, epitope peptide was more efficiently identified from gene fragment library than conventional random peptide library. Our system may be a most powerful method for identifying functional peptides.
Kawamura
Maki
M
Laboratory of Pharmaceutical Proteomics, National Institute of Biomedical Innovation, 7-6-8 Asagi, Saito, Ibaraki, Osaka 567-0085, Japan.
Shibata
Hiroko
H
Kamada
Haruhiko
H
Okamoto
Takayuki
T
Mukai
Yohei
Y
Sugita
Toshiki
T
Abe
Yasuhiro
Y
Imai
Sunao
S
Nomura
Tetsuya
T
Nagano
Kazuya
K
Mayumi
Tadanori
T
Nakagawa
Shinsaku
S
Tsutsumi
Yasuo
Y
Tsunoda
Shin-ich
SI
D000595:Amino Acid Sequence; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D018604:Epitope Mapping / Q000379:methods*; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D014409:Tumor Necrosis Factor-alpha