title: New developments in diagnosis of leishmaniasis.
abstract: In this review recent advances made in the field of human leishmaniasis have been discussed with special emphasis on the parasite, and various serological and molecular methods of diagnosing the infection. The article also reviews various modes of parasite transmission including vector borne, blood transfusion, needle sharing, sexual and person-to-person. Microbiological methods including the bone marrow, spleen, liver, lymph node aspirations and various staining methods used to demonstrate the amastigotes of the parasites and various in vitro promastigote culture methods are discussed in detail with their comparative sensitivity rates.
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine All India Institute of Medical Sciences, New Delhi, India. sarman_singh@yahoo.com
authors.forename: Sarman
authors.initials: S
authors.lastname: Singh
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000469:parasitology; D000372:Agglutination Tests; D000818:Animals; D001853:Bone Marrow / Q000469:parasitology; D006801:Humans; D016773:Leishmaniasis, Cutaneous / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology*; D007898:Leishmaniasis, Visceral / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity