Process optimization for poly-beta-hydroxybutyrate production in a nitrogen fixing cyanobacterium, Nostoc muscorum using response surface methodology.
A five-level-four-factor central composite rotary design was employed to find out the interactive effects of four variables, viz. concentrations of acetate, glucose and K2HPO4, and dark incubation period on poly-beta-hydroxybutyrate (PHB) production in a N2-fixing cyanobacterium, Nostoc muscorum. Acetate, glucose and dark incubation period exhibited positive impacts on PHB yield. Using response surface methodology (RSM), a second order polynomial equation was obtained by multiple regression analysis. A yield of 45.6% of dry cell weight (dcw) was achieved at reduced level of nutrients, i.e. 0.17% acetate, 0.16% glucose and 5 mg l(-1) K2HPO4 at a dark incubation period of 95 h as compared to 41.6% PHB yield in 0.4% acetate, 0.4% glucose and 40 mg l(-1) K2HPO4 at a dark incubation period of 168 h under single factor optimization strategy.
Sharma
Laxuman
L
Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur 721 302, India.
Kumar Singh
Akhilesh
A
Panda
Bhabatarini
B
Mallick
Nirupama
N
D000085:Acetates / Q000032:analysis; D005502:Food; D005947:Glucose / Q000032:analysis; D006885:Hydroxybutyrates / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D015233:Models, Statistical; D050502:Nostoc muscorum / Q000254:growth & development / Q000378:metabolism*; D010710:Phosphates / Q000172:deficiency; D017440:Photoperiod; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D000085:Acetates; D005947:Glucose; D006885:Hydroxybutyrates; D010710:Phosphates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates