Determination of 11 low-molecular-weight carbonyl compounds in marine algae by high-performance liquid chromatography.
A new analytical method is reported for the determination of 11 volatile carbonyl compounds isolated at room temperature from the headspace of marine algae. This method is based on the conversion of the carbonyl compounds to their 2,4-dinitrophenylhydrazone derivatives followed by high-performance liquid chromatography analysis. Using this method, 11 carbonyl compounds are detected and identified from the dynamic headspace sampling of 10 species of marine algae. Eight compounds are quantitated and the three remaining are only identified. Under optimized conditions, all carbonyl compounds are separated in 32 min. The detection and quantitation limits of the high-performance liquid chromatography method are, respectively, in the range of 0.26-0.85 ng/g of algae (formaldehyde) to 13.77-45.90 ng/g of algae (E)-2-hexenal. The calibration curves are linear in the concentration range of 2.0-1000 microg/L of solution, corresponding to 0.34-170.00 ng/g of algae. Acetaldehyde and propanal are the most abundant carbonyl compounds identified, with concentrations as high as 980 and 790 ng/g, respectively. The present work, as far as we know, is the first analytical methodology that has been developed to determine low-molecular-weight carbonyl compounds in marine algae. Because many species of marine algae are used as human food, the reported method should be useful to investigators studying the nutritional value as well as oxidative spoilage of fresh and preserved marine algae that is destined for human consumption.
da Silva
Vilma Mota
VM
Instituto de Química, Universidade Federal da Bahia--UFBA, 40170-290, Salvador, Bahia, Brazil.
da Cunha Veloso
Márcia Cristina
MC
Teixeira Sousa
Eliane
E
Santos
Gislaine Vieira
GV
Accioly
Miguel C
MC
de P Pereira
Pedro Afonso
PA
de Andrade
Jailson B
JB
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056890:Eukaryota / Q000737:chemistry*; D007659:Ketones / Q000032:analysis*; D008386:Marine Biology; D008970:Molecular Weight; D012015:Reference Standards
D007659:Ketones