title: Arginine-rich intracellular delivery peptides noncovalently transport protein into living cells.
abstract: Plasma membranes of plant or animal cells are generally impermeable to peptides or proteins. Many basic peptides have previously been investigated and covalently cross-linked with cargoes for cellular internalization. In the current study, we demonstrate that arginine-rich intracellular delivery (AID) peptides are able to deliver fluorescent proteins or beta-galactosidase enzyme into animal and plant cells, as well as animal tissue. Cellular internalization and transdermal delivery of protein could be mediated by effective and nontoxic AID peptides in a neither fusion protein nor conjugation fashion. Therefore, noncovalent AID peptides may provide a useful strategy to have active proteins function in living cells and tissues in vivo.
authors.affiliation: Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Chung-Pin
authors.initials: CP
authors.lastname: Chen
authors.forename: Ming-Huan
authors.initials: MH
authors.lastname: Chan
authors.forename: Microsugar
authors.initials: M
authors.lastname: Chang
authors.forename: Yu-Wun
authors.initials: YW
authors.lastname: Hou
authors.forename: Hwei-Hsien
authors.initials: HH
authors.lastname: Chen
authors.forename: Hui-Ru
authors.initials: HR
authors.lastname: Hsu
authors.forename: Kevin
authors.initials: K
authors.lastname: Liu
authors.forename: Han-Jung
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival; D002477:Cells / Q000166:cytology* / Q000378:metabolism*; D006224:Cricetinae; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism; D051379:Mice; D019697:Onions / Q000166:cytology / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity; D010957:Plasmids / Q000235:genetics; D021381:Protein Transport; D012867:Skin / Q000166:cytology / Q000378:metabolism
chemical_list: D010455:Peptides; D001120:Arginine