The RIN family of Ras effectors.
The human RIN1 gene was first identified as a cDNA fragment that interfered with RAS-induced phenotypes in the yeast Saccharomyces cerevisiae. Subsequent analysis of full-length RIN1 clones showed that the protein product of this gene is a downstream effector of RAS and binds with high affinity and specificity to activated HRAS. Two downstream RIN1 effector pathways have been described. The first involves direct activation of RAB5-mediated endocytosis. The second involves direct activation of ABL tyrosine kinase activity. Importantly, each of these distinct RIN1 functions is enhanced by activated RAS, suggesting that RIN1 represents a unique class of RAS effector connected to two independent signaling pathways. In this chapter, we summarize our assays and approaches for evaluating the biochemistry and biology of RIN1.
Bliss
Joanne M
JM
Department of Biological Chemistry, University of California Los Angeles School of Medicine, Los Angeles, California, USA.
Venkatesh
Byrappa
B
Colicelli
John
J
D000818:Animals; D004705:Endocytosis / Q000502:physiology; D019143:Evolution, Molecular; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; C092117:RIN1 protein, human; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D016315:Proto-Oncogene Proteins c-abl; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); D020696:rab5 GTP-Binding Proteins