title: Quenching of the electrochemiluminescence of tris(2,2'-bipyridine)ruthenium(II) by ferrocene and its potential application to quantitative DNA detection.
abstract: Efficient and stable quenching of electrochemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(II) by oxidizing ferrocene methanol (FcMeOH) at the electrode is reported. Bimolecular energy or electron transfer between Ru(bpy)(3)(2+*) and ferrocenium (Fc(+)), the oxidized species of Fc, along with suppression of radical reactions is suggested as the mechanism for quenching ECL. Fc shows more efficient quenching of ECL compared with the known quenchers phenol and 1,1-dimethyl-4,4'-bipyridine dication (MV(2+)). The ECL quenching rate constant was 5.6 x 10(10) M(-)(1) s(-)(1). Using Fc as a quencher label on a complementary DNA sequence, an intramolecular ECL quenching in hybridized oligonucleotide strands has been realized. With essentially complete quenching efficiency, this system has the potential for application to sequence-specific DNA detection.
authors.affiliation: Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA.
authors.forename: Weidong
authors.initials: W
authors.lastname: Cao
authors.forename: Jerome P
authors.initials: JP
authors.lastname: Ferrance
authors.forename: James
authors.initials: J
authors.lastname: Demas
authors.forename: James P
authors.initials: JP
authors.lastname: Landers
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D001483:Base Sequence; D056831:Coordination Complexes; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D000075163:Metallocenes; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D056831:Coordination Complexes; D004247:DNA; D005296:Ferrous Compounds; D007202:Indicators and Reagents; D000075163:Metallocenes; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds; C004998:ferrocene