White spot syndrome virus VP24 interacts with VP28 and is involved in virus infection.
White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. Herein, the characterization of VP24, a major structural protein of WSSV, is described. When purified virions were subjected to Nonidet P-40 treatment to separate the envelopes from the nucleocapsids, VP24 was found to be present exclusively in the envelope fraction. Triton X-114 extraction also indicated that VP24 behaves as an envelope protein. Immunoelectron microscopy further confirmed that VP24 is located in the virion envelope. Far-Western experiments showed that VP24 interacts with VP28, another major envelope protein of the WSSV virion. To investigate the function of VP24, WSSV was neutralized with various amounts of anti-VP24 IgG and injected into crayfish. The results showed that anti-VP24 IgG could partially attenuate infection with WSSV. It is concluded that VP24 is an envelope protein and functions at an early stage in virus infection.
Xie
Xixian
X
School of Life Science, Xiamen University, 178 Daxue Road, Xiamen 361005, People's Republic of China.
Yang
Feng
F
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Xiamen, People's Republic of China.
D000818:Animals; D003400:Astacoidea; D001483:Base Sequence; D004266:DNA Virus Infections / Q000209:etiology; D004279:DNA, Viral / Q000235:genetics; D016253:Microscopy, Immunoelectron; D009500:Neutralization Tests; D033561:Penaeidae; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D014774:Virulence; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology*
D004279:DNA, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins