Extended storage of platelets in SSP platelet additive solution.
OBJECTIVE
The aim of this study was to investigate the effects of extended storage of pooled random platelets in SSP+ additive solution (MacoPharma).


METHODS
Eight buffy coat-derived, pooled, leucoreduced platelet concentrates were prepared in 75% SSP+, 25% plasma using Fresenius/NPBI Composelect thrombocyte polishing filter (TPF) systems. Platelet concentrates were stored for 19 days in polyolefin storage bags and samples for in vitro analysis were taken at various time-points during storage.


RESULTS
Platelet yields were lower than seen routinely when platelets are prepared in 100% plasma. The in vitro quality of the platelets stored in SSP+ was maintained until day 9. Glucose was depleted by day 12 and this was accompanied by a rapid fall in pCO2, a rise in pO2 and a cessation of lactate production. ATP and bicarbonate concentrations fell, the platelets began to swell and the ability to swirl decreased. Soluble P-selectin, glycocalicin, and regulated on activation, normal, T-cell expressed, and secreted (RANTES) concentrations increased, as did P-selectin expression. Loss of platelets and an increase in lacate hydrogenase concentration indicated that lysis had occurred. However, the pH remained between 6.4 and 7.4.


CONCLUSIONS
The results suggest that SSP+ could be used for platelet storage for up to 9 days. However, the preparation of platelets in the additive requires some optimization. In vivo studies are required to confirm these in vitro results.
Hornsey
V S
VS
Products and Components R&D Group, National Science Laboratory, Scottish National Blood Transfusion Service, Edinburgh, UK. valerie.hornsey@snbts.csa.scot.nhs.uk
McColl
K
K
Drummond
O
O
McMillan
L
L
Morrison
A
A
Morrison
L
L
MacGregor
I R
IR
Prowse
C V
CV
D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D001793:Blood Preservation; D006801:Humans; D019999:Pharmaceutical Solutions / Q000737:chemistry; D013997:Time Factors
D019999:Pharmaceutical Solutions