The in vitro viability and growth of fibroblasts cultured in the presence of different bone grafting materials (NanoBone and Straumann Bone Ceramic).
Different clinical applications, including dentistry, are making increasing demands on bone grafting material. In the present study we have analysed the viability, proliferation and growth characteristics of fibroblasts cultured in vitro together with two different bone grafting materials, NanoBone and Straumann Bone Ceramic, over a period of 24 and 28 days respectively. Viability was measured at least every 72 hours by using the alamarBlue assay, a test that measures quantitatively cell proliferation and viability but does not require cell fixation or extraction. After one week of culture fibroblast viability was as high as in controls for both grafting materials and remained high (> 90%) for the duration of the experiment. Cell growth was evaluated microscopically. Scanning electron microscopy revealed a dense fibroblast growth at the surface of both bone grafting materials after three weeks of in vitro culture. Generally, our in vitro analyses contribute to further insights into cell - scaffold interactions.
Kauschke
E
E
Institute of Anatomy and Cell Biology, Ernst Moritz Arndt University, Greifswald, Germany. kauschke@uni-greifswald.de
Rumpel
E
E
Fanghänel
J
J
Bayerlein
T
T
Gedrange
T
T
Proff
P
P
D000818:Animals; D018786:Bone Substitutes / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010078:Oxazines / Q000378:metabolism; D014966:Xanthenes / Q000378:metabolism
D018786:Bone Substitutes; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin