title: Defective mitochondrial gene expression results in reactive oxygen species-mediated inhibition of respiration and reduction of yeast life span.
abstract: Mitochondrial dysfunction causes numerous human diseases and is widely believed to be involved in aging. However, mechanisms through which compromised mitochondrial gene expression elicits the reported variety of cellular defects remain unclear. The amino-terminal domain (ATD) of yeast mitochondrial RNA polymerase is required to couple transcription to translation during expression of mitochondrial DNA-encoded oxidative phosphorylation subunits. Here we report that several ATD mutants exhibit reduced chronological life span. The most severe of these (harboring the rpo41-R129D mutation) displays imbalanced mitochondrial translation, conditional inactivation of respiration, elevated production of reactive oxygen species (ROS), and increased oxidative stress. Reduction of ROS, via overexpression of superoxide dismutase (SOD1 or SOD2 product), not only greatly extends the life span of this mutant but also increases its ability to respire. Another ATD mutant with similarly reduced respiration (rpo41-D152A/D154A) accumulates only intermediate levels of ROS and has a less severe life span defect that is not rescued by SOD. Altogether, our results provide compelling evidence for the "vicious cycle" of mitochondrial ROS production and lead us to propose that the amount of ROS generated depends on the precise nature of the mitochondrial gene expression defect and initiates a downward spiral of oxidative stress only if a critical threshold is crossed.
authors.affiliation: Department of Pathology, Yale University School of Medicine, 310 Cedar St., P.O. Box 208023, New Haven, CT 06520-8023, USA.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Bonawitz
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Rodeheffer
authors.forename: Gerald S
authors.initials: GS
authors.lastname: Shadel
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D050259:Genes, Mitochondrial; D024101:Mitochondrial Proteins; D009154:Mutation; D018384:Oxidative Stress / Q000235:genetics*; D010641:Phenotype; D014176:Protein Biosynthesis / Q000235:genetics; D012313:RNA / Q000378:metabolism; D000077278:RNA, Mitochondrial; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000099156:Superoxide Dismutase 2
chemical_list: D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases; D024101:Mitochondrial Proteins; D012313:RNA; D000077278:RNA, Mitochondrial; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D014157:Transcription Factors; D000099156:Superoxide Dismutase 2; C081937:MSN4 protein, S cerevisiae; C000606290:SOD1 protein, human; C487996:RPO41 protein, S cerevisiae