The interaction between tylophorine B and TMV RNA.
Tylophorine B exhibits 60% inhibition against tobacco mosaic virus (TMV) at a concentration of 1.0 x 10(-6) g/ml. In our study, high affinity for TMV RNA and assembly origin of TMV RNA (oriRNA) was revealed, accompanied by the conformational change of RNA. Considering that TMV assembly begins with the specific recognition by the coat protein aggregate of oriRNA, and that tylophorine B has favorable interaction with oriRNA, we speculate that tylophorine B likely exerts its virus inhibition by binding to oriRNA and interfering with virus assembly initiation. This work may shed light on the possible molecular inhibition mechanism against TMV by tylophorine B, and provide clues in rational design of sequence-specific RNA binding antivirus drugs.
Xi
Zhen
Z
State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, Nankai University, Tianjin, China. zhenxi@nankai.edu.cn
Zhang
Ruoyu
R
Yu
Zhihong
Z
Ouyang
Di
D
D000470:Alkaloids / Q000737:chemistry*; D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D002942:Circular Dichroism; D007212:Indolizines / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010616:Phenanthrenes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D012367:RNA, Viral / Q000235:genetics*; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D019065:Virus Assembly; D016662:Virus Integration
D000470:Alkaloids; D000998:Antiviral Agents; D007212:Indolizines; D010616:Phenanthrenes; D012367:RNA, Viral; C408668:tylophorine B; D012313:RNA