The proteome of Mannheimia succiniciproducens, a capnophilic rumen bacterium.
Mannheimia succiniciproducens MBEL55E isolated from bovine rumen is an industrially important bacterium as an efficient succinic acid producer. Recently, its full genome sequence was determined. In the present study, we analyzed the M. succiniciproducens proteome based on the genome information using 2-DE and MS. We established proteome reference map of M. succiniciproducens by analyzing whole cellular proteins, membrane proteins, and secreted proteins. More than 200 proteins were identified and characterized by MS/MS supported by various bioinformatic tools. The presence of proteins previously annotated as hypothetical proteins or proteins having putative functions were also confirmed. Based on the proteome reference map, cells in the different growth phases were analyzed at the proteome level. Comparative proteome profiling revealed valuable information to understand physiological changes during growth, and subsequently suggested target genes to be manipulated for the strain improvement.
Lee
Jeong Wook
JW
Department of Chemical & Biomolecular Engineering, Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
Lee
Sang Yup
SY
Song
Hyohak
H
Yoo
Jong-Shin
JS
D000818:Animals; D002417:Cattle / Q000382:microbiology*; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D005285:Fermentation; D016680:Genome, Bacterial; D031121:Mannheimia / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D013058:Mass Spectrometry; D008954:Models, Biological; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D012417:Rumen / Q000382:microbiology*
D010446:Peptide Fragments; D020543:Proteome