The guanidino-group modifying enzymes: structural basis for their diversity and commonality.
The guanidino-group modifying enzyme (GME) superfamily contains many drug targets, including metabolic enzymes from pathogenic microorganisms as well as key regulatory proteins from higher eukaryotes. These enzymes, despite their diverse sequences, adopt the common alpha/beta propeller fold and catalyze the modification of (methylated) guanidino groups. Our structural superposition and structure-based alignment for the GMEs have identified key residues that are involved in the catalysis and substrate binding. We have shown that conserved guanidino-carboxyl interactions are utilized in two different ways; the acidic residues in the catalytic site form hydrogen bonds to the substrate guanidino group, and the enzyme Arg residues at several key positions recognize the carboxyl group of the substrate and fix its orientation. Based on this observation, we have proposed rules for classifying the GME sequences and predicting their molecular function from the conservation of the key acidic and Arg residues. Other novel motifs have been identified, which involve residues that are not in direct contact with the substrate but are likely to stabilize the active-site conformation through hydrogen-bonding networks. In addition, we have examined the domain architecture of the GMEs. Although most members consist of a single catalytic domain, fold recognition analysis has identified a likely bifunctional enzyme from a cyanobacterium. It has also revealed common immunoglobulin-like beta-sandwich domains found in the enzymes that recognize protein substrates. These findings will be useful for predicting the precise mechanism of action for potential novel targets and designing therapeutic compounds against them.
Shirai
Hiroki
H
Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
Mokrab
Younes
Y
Mizuguchi
Kenji
K
D000579:Amidinotransferases / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D000581:Amidohydrolases / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D006146:Guanidines / Q000378:metabolism*; D006801:Humans; D006860:Hydrogen Bonding; D006867:Hydrolases / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D000076342:Protein-Arginine Deiminases; D016415:Sequence Alignment
D006146:Guanidines; D000579:Amidinotransferases; C020681:glycine amidinotransferase; D006867:Hydrolases; D000581:Amidohydrolases; D000076342:Protein-Arginine Deiminases; C059999:dimethylargininase