title: Effects of dietary supplementation with clostridium butyricum on the growth performance and humoral immune response in Miichthys miiuy.
abstract: The effects of dietary supplementation with Clostridium butyricum on growth performance and humoral immune response in Miichthys miiuy were evaluated. One hundred and fifty Miichthys miiuy weighing approximately 200-260 g were divided into five groups and reared in 15 tanks with closed circuiting culture system. The animals were fed 5 diets: basal diet only (control) or supplemented of the basal diet with C. butyricum at doses of 10(3) (CB1), 10(5) (CB2), 10(7) (CB3) or 10(9) (CB4) CFU/g. Compared with the control, the serum phenoloxidase activity was significantly increased by the supplementation (P<0.05), acid phosphatases activity was increased significantly (P<0.05) at the doses of 10(9) CFU/g. Serum lysozyme activity peaked at dose of 10(7) CFU/g and in the skin mucus at dose of 10(9) CFU/g. Immunoglobulin M level in the serum and skin mucus was increased except at dose of 10(3) CFU/g (P<0.05). The growth at the dose of 10(9) CFU/g was higher than that of the control (P<0.05). It is concluded that supplementation of C. butyricum can mediate the humoral immune responses and improve the growth performance in Miichthys miiuy.
authors.forename: Zeng-fu
authors.initials: ZF
authors.lastname: Song
authors.forename: Tian-xing
authors.initials: TX
authors.lastname: Wu
authors.forename: Li-sheng
authors.initials: LS
authors.lastname: Cai
authors.forename: Li-jing
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Zheng
mesh_terms: D000821:Animal Feed / Q000382:microbiology*; D000818:Animals; D000917:Antibody Formation / Q000502:physiology*; D046972:Clostridium butyricum / Q000276:immunology*; D019587:Dietary Supplements / Q000382:microbiology*; D005399:Fishes / Q000254:growth & development* / Q000276:immunology*; D019936:Probiotics / Q000008:administration & dosage*