Variation in Trypanosoma cruzi clonal composition detected in blood patients and xenodiagnosis triatomines: implications in the molecular epidemiology of Chile.
To identify Trypanosoma cruzi clones from chronically infected individuals, they were transferred to triatomines by the xenodiagnosis test (XD) with Triatoma infestans. Polymerase chain reaction (PCR) and hybridization assays were performed to detect minicircle DNA in human blood samples and triatomine feces, using probes to determine the T. cruzi clones present. T. cruzi clone 19 (TcI) resulted the most prevalent in humans, with a frequency of 0.70 compared with a frequency of 0.53 in triatomines. T. cruzi clone 39 (TcIId) was the most prevalent in T. infestans, with a frequency of 0.65 compared with 0.33 in humans. The T. cruzi clone 43 (TcIIe) was not detected in blood samples; nevertheless, it was present at a rate of 0.17 in T. infestans feces. In conclusion, the T. cruzi clones are associated to each host, suggesting that selective amplification of clones occurs in human and triatomines.
Coronado
Ximena
X
Program of Cellular and Molecular Biology, Biomedical Sciences Institute, Faculty of Medicine, University of Chile, Santiago, Chile.
Zulantay
Ines
I
Albrecht
Helena
H
Rozas
Marlene
M
Apt
Werner
W
Ortiz
Sylvia
S
Rodriguez
Jorge
J
Sanchez
Gittith
G
Solari
Aldo
A
D000328:Adult; D000818:Animals; D014355:Chagas Disease / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission; D002677:Chile / Q000453:epidemiology; D002999:Clone Cells / Q000145:classification; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes; D018105:DNA, Kinetoplast / Q000097:blood / Q000302:isolation & purification; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods / Q000592:standards*; D016133:Polymerase Chain Reaction / Q000379:methods; D014225:Triatominae / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics*; D020525:Xenodiagnosis / Q000379:methods
D017931:DNA Primers; D015342:DNA Probes; D018105:DNA, Kinetoplast