Enhanced protective immunity against pneumococcal infection with PspA DNA and protein.
The effect of priming and boosting with pspA/EF5668 and purified recombinant PspA/EF5668 was examined. With this strategy CBA/N mice were protected against fatal challenge with Streptococcus pneumoniae EF5668. Anti-PspA antibody titers were elevated, and Western analysis with the immune serum demonstrated cross-reactivity with PspA from several different pneumococcal isolates, representing different PspA clades. Immune serum localized cross-reactive epitopes to the alpha-helical domain of PspA/Rx1 and PspA/EF5668. We demonstrated that DNA/protein prime-boost immunizations can enhance protective immunity against pneumococcal challenge.
Moore
Quincy C
QC
Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, United States.
Bosarge
Joseph R
JR
Quin
Lisa R
LR
McDaniel
Larry S
LS
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007117:Immunization, Secondary; D051379:Mice; D008808:Mice, Inbred CBA; D011008:Pneumococcal Infections / Q000276:immunology / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology* / Q000472:pathogenicity; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D022242:Pneumococcal Vaccines; D019444:Vaccines, DNA; C051592:pneumococcal surface protein A