Physiological and behavioral effects of chemoreceptors located in different body parts of the swimming crab Callinectes danae.
By perfusing their branchial chambers with filtered seawater, we have developed a preparation that allows us to maintain the swimming crab Callinectes danae outside water without any major effects on its cardiac activity. This in turn allowed us to selectively stimulate chemoreceptors located in different body parts, and specifically to discriminate between the receptors located in the branchial chambers and those located in the oral region (mainly in the mouthparts, antennules and antennae). We show that a taurine solution can evoke bradycardia when applied to the oral region or to a combination of the oral region and the branchial chambers. Although the precise localization of the oral region receptors involved remains to be determined, ablation experiments show that the olfactory organs (i.e., the antennules) are not involved. Finally, we show that although stimulating the pereiopods has no effect on the animals' cardiac activity it causes the animals to move, putatively to try to grasp a piece of food, a reaction not evoked by stimulating the gills or the oral regions. Our results lend support to the idea that chemoreceptors located in different parts of the body play different functional roles in decapod crustaceans.
Aggio
Juan F
JF
Instituto de Biociências, Universidade de São Paulo, Brazil. jfaggio@whitney.ufl.edu
de Freitas
José C
JC
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D003386:Brachyura / Q000502:physiology*; D002628:Chemoreceptor Cells / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006321:Heart / Q000502:physiology; D006339:Heart Rate; D008297:Male; D009043:Motor Activity; D009055:Mouth / Q000502:physiology; D013654:Taurine / Q000494:pharmacology
D013654:Taurine