Molecular characterization of recombinant Hepatitis B surface antigen from Chinese hamster ovary and Hansenula polymorpha cells by high-performance size exclusion chromatography and multi-angle laser light scattering.
The molecular weight and size of recombinant Hepatitis B surface antigen (HBsAg) derived from Chinese hamster ovary (CHO) and the Hansenula polymorph have been characterized by high-performance size exclusion chromatography with multi-angle laser light scattering (HPSEC-MALLS). The average molecular weight of CHO-derived HBsAg particle (CHO-rHBsAg) (4921 kDa) was higher than that of H. polymorpha yeast strain (Hans-rHBsAg) (3010 kDa). The size of CHO-rHBsAg (22.1 nm) is nearly the same as that of native HBsAg compared to 18.1 nm for Hans-rHBsAg. The average monomer numbers were found to be 155 for CHO-rHBsAg and 86 for Hans-rHBsAg, respectively. The data obtained support the assumption that the higher immunogenicity of CHO-derived HBsAg is related to its more favorable macromolecular assembly structure.
Zhou
Weibin
W
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, PR China.
Bi
Jingxiu
J
Janson
Jan-Christer
JC
Li
Yan
Y
Huang
Yongdong
Y
Zhang
Yan
Y
Su
Zhiguo
Z
D000818:Animals; D016466:CHO Cells; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007834:Lasers; D008970:Molecular Weight; D010843:Pichia / Q000166:cytology / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012542:Scattering, Radiation
D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins