title: Efficient transformation of Legionella pneumophila by high-voltage electroporation.
abstract: A simple and reproducible method has been developed to transform Legionella pneumophila by electroporation. Effects of different conditions, including electric field strength, pulse length, DNA quality and cell density, were evaluated. Using our method, an efficiency of up to 6 x 10(7) transformants/microg DNA was obtained. This optimized transformation procedure should efficiently facilitate gene manipulations in L. pneumophila, such as plasmid transfer, transposon mutagenesis, library transformation for complementation cloning, etc.
authors.affiliation: Department of Biochemistry and State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen Zhongshan University, Guangzhou, China.
authors.forename: Ding-Qiang
authors.initials: DQ
authors.lastname: Chen
authors.forename: Shao-Song
authors.initials: SS
authors.lastname: Huang
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Lu
mesh_terms: D004492:Edetic Acid; D018274:Electroporation / Q000379:methods*; D016952:Legionella pneumophila / Q000235:genetics*; D010957:Plasmids; D014169:Transformation, Bacterial
chemical_list: D004492:Edetic Acid