title: Diversity, complexity and transmission of double-stranded RNA elements in Chalara elegans (synanam. Thielaviopsis basicola).
abstract: Double-stranded (ds) RNA banding patterns were determined in 21 wild-type strains of the soilborne plant pathogen Chalara elegans originating from different geographic regions worldwide. Five strains, each with a unique dsRNA pattern, were selected for cDNA cloning, northern blot analysis and dsRNA transmission experiments. Four strains contained multiple (up to 6) dsRNA elements (2.0kbp to 12kbp in size) and one strain contained a single 2.8kbp fragment. These five strains were distinguished from one another by their unique RAPD-PCR patterns. Seven partial cDNA clones were derived from the predominant 2.8, 5.3, and 12kbp dsRNA elements. Nucleotide sequence analysis and northern blot hybridizations revealed a high degree of genetic dissimilarity among the different molecular-size dsRNA elements, even those found within a single strain. Four clones from the 5.3kbp dsRNA fragment showed a 23-43 % amino acid identity to either the coat protein or RNA-dependent RNA polymerase regions of viruses in the Totiviridae. One clone from the 2.8kbp dsRNA fragment had a 55-57 % amino acid identity to the RdRp region of viruses in the Narnaviridae. Two clones from the 12kbp dsRNA fragment showed no significant homology to any known virus group. Colonies derived from 100 single-conidia isolates of C. elegans strains with the 2.8, 5.3 and 12kbp elements all contained the corresponding dsRNA element, indicating that dsRNA transmission through conidia was highly efficient, regardless of molecular size. However, transmission of dsRNA between the mycelium of strains of C. elegans could not be achieved in this study. Genetically unique strains carrying diverse dsRNA elements appear to have evolved within populations of C. elegans. Based on our findings, there are at least 3 groups of viruses present in C. elegans.
authors.affiliation: Department of Biological Sciences, Centre for Environmental Biology, Simon Fraser University, 888 University Drive, Burnaby, BC, V5A 1S6, Canada.
authors.forename: Yunjung
authors.initials: Y
authors.lastname: Park
authors.forename: Xiaobang
authors.initials: X
authors.lastname: Chen
authors.forename: Zamir K
authors.initials: ZK
authors.lastname: Punja
mesh_terms: D001203:Ascomycota / Q000821:virology*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012328:RNA Viruses / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D019196:Totiviridae / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D012330:RNA, Double-Stranded