title: [Protective effect of maternal immunoglobulin G against NMDA-induced neurotoxicity on hippocampus neurons].
abstract: OBJECTIVE
  To investigate possible protective effect of maternal immunoglobulin G (IgG) against N-methyl-D-aspartate-mediated neurotoxicity on primary-cultured rat hippocampal neurons and the mechanism of the effect.
  
  
  METHODS
  An in vitro system had been developed for the study of hippocampal neurons. Intracellular lactic dehydrogenase (LDH) release was used as a marker to measure the rates of neuronal damage. The cells were stained with Trypan blue to measure the rate of neuronal death.
  
  
  RESULTS
  N-methyl-D-aspartate (NMDA) at a concentration of 50 micromol/L resulted in increased release of LDH and the cell mortality (P < 0.01, respectively). Maternal IgG of different concentration (10 mg/L, 100 mg/L) inhibited NMDA-induced intracellular LDH release (P < 0.01, respectively) and cell mortality (P < 0.05, 0.01, respectively), and larger dose had stronger effect (P < 0.05).
  
  
  CONCLUSIONS
  Maternal IgG had protective effect on primary-cultured rat hippocampal neurons injured by NMDA and the effect was dose-dependent.
authors.affiliation: Department of Neurology and Rehabilitation Unit, Beijing Children's Hospital, Capital University of Medical Sciences, Beijing 100045, China.
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Wang
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zou
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Zeng
authors.forename: Chun-li
authors.initials: CL
authors.lastname: Zhao
authors.forename: Jun-yan
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018690:Excitatory Amino Acid Agonists; D005260:Female; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007112:Immunity, Maternally-Acquired / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D007155:Immunologic Factors / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000096:biosynthesis; D008297:Male; D016202:N-Methylaspartate; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009924:Organ Culture Techniques; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018690:Excitatory Amino Acid Agonists; D007074:Immunoglobulin G; D007155:Immunologic Factors; D016202:N-Methylaspartate; D007770:L-Lactate Dehydrogenase