title: [Clinicopathological significance of aberrant methylation of the fragile histidine triad gene in patients with hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the aberrant methylation of fragile histidine triad (FHIT) gene and to explore possible relationship between the aberrant methylation of FHIT and clinicopathological features in hepatocellular carcinoma (HCC).
  
  
  METHODS
  The hypermethylation of FHIT was detected by the methylation specific PCR (MSP) method in 45 patients with HCC (tumoral and nontumoral tissue), 14 cases of normal livers and 4 HCC cell lines (SK-Hep-1, Hep-G2, Hep-3B and Huh7). The correlation of FHIT methylation and clinicopathological features was analyzed.
  
  
  RESULTS
  The frequencies of hypermethylation of FHIT in tumoral and nontumoral tissue, normal liver and cell lines were 71.1%, 64.4%, 14.3% and 75.0%, respectively. A significant relation between hypermethylation of FHIT and poor survival was present (P = 0.0430).
  
  
  CONCLUSIONS
  Hypermethylation of FHIT is a frequent and early event in HCC, it might relate to a poor prognosis for patients with HCC.
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Geng
authors.forename: Li-xin
authors.initials: LX
authors.lastname: Zhu
authors.forename: Qi-ru
authors.initials: QR
authors.lastname: Xiong
authors.forename: Ye-ben
authors.initials: YB
authors.lastname: Qian
authors.forename: Gui-yin
authors.initials: GY
authors.lastname: Dong
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Li
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D011379:Prognosis; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase