A simple and practical agglutination assay for human leucocyte antigen-B27 typing.
OBJECTIVE
The human leucocyte antigen (HLA) B27 is the most frequently typed single antigen that is associated with diseases. Here, we describe a simple and rapid particle agglutination assay (PaGIA) for HLA-B27 typing.


METHODS
Superparamagnetic particles were coated with a monoclonal antibody to HLA-B27 and subsequently used for testing. Anticoagulated whole-blood samples were obtained from healthy blood donors (n = 194) with known HLA patterns and from patients (n = 51) who had been typed positive for HLA-B27 by flow cytometry.


RESULTS
The particles agglutinated only after incubation with HLA-27-positive blood samples, using the ID-microtyping system. Positive reactions were clearly distinguishable from negative reactions in all samples tested. Flow cytometric HLA-B27 typing revealed an indeterminate result in one patient.


CONCLUSIONS
The new HLA-B27 PaGIA is suitable for rapid typing of HLA-B27. The assay is simple and easy to perform, and can be implemented in any routine laboratory.
Meyer
O
O
Charité, Universitätsmedizin Berlin, Institut für Transfusionsmedizin, Berlin, Germany.
Abdallah
Z
Z
Schönemann
C
C
Hugo
F
F
Agaylan
A
A
Kiesewetter
H
H
Salama
A
A
D000372:Agglutination Tests / Q000379:methods*; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D015796:HLA-B27 Antigen / Q000032:analysis* / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D007841:Latex Fixation Tests; D012680:Sensitivity and Specificity; D013167:Spondylitis, Ankylosing / Q000175:diagnosis
D000911:Antibodies, Monoclonal; D015796:HLA-B27 Antigen