title: Yeast elf1 factor is phosphorylated and interacts with protein kinase CK2.
abstract: One of the biggest group of proteins influenced by protein kinase CK2 is formed by factors engaged in gene expression. Here we have reported recently identified yeast transcription elongation factor Elf1 as a new substrate for both monomeric and tetrameric forms of CK2. Elf1 serves as a substrate for both the recombinant CK2alpha' (K(m) 0.38 microM) and holoenzyme (K(m) 0.13 microM). By MALDI-MS we identified the two serine residues at positions 95 and 117 as the most probable in vitro phosphorylation sites. Coimmunoprecypitation experiments show that Elf1 interacts with catalytic (alpha and alpha') as well as regulatory (beta and beta') subunits of CK2. These data may help to elucidate the role of protein kinase CK2 and Elf1 in the regulation of transcription elongation.
authors.affiliation: Department of Molecular Biology, Environmental Protection Institute, John Paul II Catholic University of Lublin, Poland.
authors.forename: Konrad
authors.initials: K
authors.lastname: Kubinski
authors.forename: Rafal
authors.initials: R
authors.lastname: Zielinski
authors.forename: Ulf
authors.initials: U
authors.lastname: Hellman
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Mazur
authors.forename: Ryszard
authors.initials: R
authors.lastname: Szyszka
mesh_terms: D000595:Amino Acid Sequence; D047390:Casein Kinase II / Q000737:chemistry / Q000378:metabolism*; D020134:Catalytic Domain; D005656:Fungal Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D035602:Transcriptional Elongation Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: C506065:Elf1 protein, S cerevisiae; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D035602:Transcriptional Elongation Factors; D047390:Casein Kinase II