Tube-immunoassay for rapid detection of carbaryl residues in agricultural products.
An antibody-based rapid, quantitative, and qualitative tube enzyme-linked immunosorbent assay (tube-ELISA) was developed and used to determine carbaryl (1-naphthyl methylcarbamate) residues in agricultural products (apple, Chinese cabbage, rice, and barley). The tube-ELISA is a competitive immunoassay in which the antibody is coated in the polystyrene tube, with a dynamic range between 0.7 and 46.3 microg kg(-1). Carbaryl was extracted from each agricultural sample by hand-shaking with methanol and examined for application to on-site analysis. After the liquid extraction, the sample extracts diluted with buffer were analyzed by rapid tube-ELISA directly. The overall test time was around 15-30 min, including sample preparation and assay performance. The results obtained from tube-ELISA correlated well with high-performance liquid chromatography (R2 > 0.9). The study shows that tube-ELISA is useful as a quality control tool and can be used to quantitatively detect carbaryl as well.
Wang
Shuo
S
Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, P.R. China. s.wang@tust.edu.cn
Yu
Chundi
C
Zhang
Yan
Y
Wang
Junping
J
Duan
Zhenjuan
Z
Zhang
Juankun
J
D012721:Carbaryl / Q000032:analysis*; D003257:Consumer Product Safety; D018556:Crops, Agricultural / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D006801:Humans; D007306:Insecticides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D012680:Sensitivity and Specificity
D007306:Insecticides; D010573:Pesticide Residues; D012721:Carbaryl