Expression of bovine viral diarrhea virus glycoprotein E2 as a soluble secreted form in a Mammalian cell line.
Bovine viral diarrhea virus (BVDV) membrane-anchored type I glycoprotein E2 is an approximately 53-kDa immunodominant glycoprotein inducing the production of neutralizing antibodies in the animal host after natural infection or following immunization with live or killed vaccines. The E2 coding region lacking the transmembrane domain was constructed in a soluble secreted form (secE2) and expressed in the medium of a transiently transfected human cell line. The crude conditioned medium containing secE2 can be potentially employed to develop an enzyme-linked immunosorbent assay antigen for the diagnosis of BVDV infection or for vaccine purposes.
Donofrio
Gaetano
G
Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali, Facoltà di Medicina Veterinaria, Via del Taglio 8, 43100 Parma, Italy. gaetano.donofrio@unipr.it
Bottarelli
Ezio
E
Sandro
Cavirani
C
Flammini
Cesidio Filippo
CF
D000818:Animals; D015153:Blotting, Western; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000378:metabolism*; D002417:Cattle; D002460:Cell Line; D001908:Diarrhea Viruses, Bovine Viral / Q000737:chemistry*; D015870:Gene Expression / Q000502:physiology*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009500:Neutralization Tests / Q000379:methods; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
D006023:Glycoproteins; D014759:Viral Envelope Proteins