The pattern of E2F1 and c-myb immunoreactivities in the CA1 region is different from those in the CA2/3 region of the gerbil hippocampus induced by transient ischemia.
In this study, we examined transient ischemia-induced changes in transcription factor E2F1 and c-myb expressions in the gerbil hippocampus after 5 min of transient forebrain ischemia. E2F1 immunoreactivity significantly increased in the CA1 region 6-12 h after ischemia/reperfusion. c-myb immunoreactivity increased mainly in CA1 pyramidal cells with time by 12 h after ischemia. Thereafter, E2F1 and c-myb immunoreactivities significantly decreased compared to those in the 12 h post-ischemic group. Four days after ischemia/reperfusion, E2F1 and c-myb immunoreactivities were detected in non-pyramidal cells. Ten days after ischemia, c-myb immunoreactivity increased again: at this time, astrocytes as well as non-pyramidal cells showed E2F1 and c-myb immunoreactivities. In the CA2/3 region, E2F1 and c-myb immunoreactivities mainly changed in non-pyramidal cells, and 10 days after ischemia, c-myb immunoreactivity was not expressed in astrocytes. In conclusion, E2F1 and c-myb significantly alter in pyramidal cells and express in astrocytes in the gerbil hippocampal CA1 region after transient ischemia. These results indicate that E2F1 and c-myb in the CA1 region after ischemic damage may be associated with delayed neuronal death.
Hwang
In Koo
IK
Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, South Korea.
Yoo
Ki-Yeon
KY
Cho
Byung Moon
BM
Hwang
Hyung Sik
HS
Kim
Sung Min
SM
Oh
Sae Moon
SM
Choi
Sun Kil
SK
Hwang
Do Yun
DY
Won
Moo Ho
MH
Moon
Seung Myung
SM
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D016923:Cell Death / Q000502:physiology; D050703:E2F2 Transcription Factor / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology*; D007150:Immunohistochemistry / Q000379:methods; D002546:Ischemic Attack, Transient / Q000473:pathology*; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D020598:Proto-Oncogene Proteins c-myb / Q000378:metabolism*; D015424:Reperfusion; D013997:Time Factors
D050703:E2F2 Transcription Factor; D020598:Proto-Oncogene Proteins c-myb