Microsatellite analysis of three phylogenetic species of Paracoccidioides brasiliensis.
Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an important human systemic mycosis in Latin America. Recently, the existence of three different phylogenetic species (S1, PS2, and PS3) of P. brasiliensis was demonstrated. Despite being genetically isolated, all three species were capable of inducing disease in both humans and animals, although lower virulence has been found with the PS2 species. The available molecular methods developed to characterize and type strains have not been useful for assigning isolates to the described species, creating the need for molecular markers capable of distinguishing genetically isolated groups. Here, we describe a PCR and sequencing-based microsatellite marker system that is stable, easy to assay, adaptable to large series of isolates, and discriminatory enough to be used as a typing system in identifying the three proposed species of P. brasiliensis. In addition, this system provides an unambiguous tool for strain discrimination between two (S1 and PS2) of the three phylogenetic species.
Matute
Daniel R
DR
Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia.
Sepulveda
Victoria E
VE
Quesada
Lina M
LM
Goldman
Gustavo H
GH
Taylor
John W
JW
Restrepo
Angela
A
McEwen
Juan G
JG
D000483:Alleles; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010228:Paracoccidioides / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D005819:Genetic Markers