title: [cDNA microarray-based study of gene expression profile changes in human esophageal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the differentially expressed genes between human esophageal squamous cell carcinoma (ESCC) and normal esophageal mucosa and explore an effective method with high throughput for screening the molecular markers closely correlated with the development, invasion and metastasis of ESCC.
  
  
  METHODS
  With cDNA microarray and laser capture microdissection, T7-based amplification were used to detect the mRNA from both the primary carcinoma and the corresponding esophageal epithelium in 15 ESCC cases, and the results were analyzed by bioinformatics methods.
  
  
  RESULTS
  Among the 886 target genes, 110 (12.42%) genes were differentially expressed commonly at least twice in all the 15 samples, including 56 (6.32%) up-regulated by at least 2 folds and 54 (6.09%) down-regulated by at least 0.5 folds.
  
  
  CONCLUSIONS
  Many ESCC-associated genes were screened by the high-throughput gene chip method, and functional study of these genes may help to identify the key genes or pathways involved in the pathogenesis and development of ESCC.
authors.affiliation: Department of Pathophysiology, School for Basic Medical Sciences of Zhengzhou University, Zhengzhou 450052, China. lipeifreemai@hotmail.com
authors.forename: Pei
authors.initials: P
authors.lastname: Li
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Ling
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Yang
authors.forename: You-tian
authors.initials: YT
authors.lastname: Huang
authors.forename: Ji-min
authors.initials: JM
authors.lastname: Zhao
authors.forename: Ming-yao
authors.initials: MY
authors.lastname: Zhao
authors.forename: Zi-ming
authors.initials: ZM
authors.lastname: Dong
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004848:Epithelium / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D004947:Esophagus / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019859:Proto-Oncogene Proteins c-met; D017978:Receptors, Growth Factor / Q000235:genetics
chemical_list: D011518:Proto-Oncogene Proteins; D017978:Receptors, Growth Factor; C491743:MET protein, human; D019859:Proto-Oncogene Proteins c-met