[Investigation of algae pollution in Xiliu Lake and identification of toxic cyanobacteria by whole-cell PCR].
OBJECTIVE
To investigate the contaminative condition of the floating algae (especially toxic cyanobacteria) in Xiliu Lake, and establish a whole-cell PCR method for identifying the toxic cyanobacteria.


METHODS
The surface water of Xiliu Lake was sampled by plastic sampler from March, 2004, and the number of algae was counted by using blood cell counter. The phycocyanin intergenic spacer region (PC-IGS) and microcystin synthetase gene B (mcyB) were identified by whole-cell PCR in water samples, and the amplified product of mcyB was inserted into T vector and sequenced.


RESULTS
Cyanobacteria, Chlorophyta, Bacillariophyta and Euglenophyta were main algae, and cyanobacteria was the dominant algae in summer and autumn. From July 7 to September 27,2 004, PC-IGS was detected positively in 11 samples, and from July 29 to September 27, mcyB was-detieted positively in 9 samples. Compared with the reported mcyB of Microcystis aeruginosa in Genbank, the homology of gene sequence was more than 97 t he homology of amino acid sequence was more than 94%.


CONCLUSIONS
In summer and autumn toxic cyanobacteria could be detected in Xiliu Lake. Toxic cyanobacteria could be identified successfully by whole-cell PCR.
Ban
Hai-qun
HQ
School of Public Health, Zhengzhou University, Zhengzhou 450052, China.
Zhuang
Dong-gang
DG
Zhu
Jing-yuan
JY
Ba
Yue
Y
D002681:China; D000458:Cyanobacteria / Q000235:genetics / Q000302:isolation & purification*; D056890:Eukaryota; D005618:Fresh Water / Q000382:microbiology*; D052998:Microcystins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D014871:Water Microbiology; D014876:Water Pollution / Q000032:analysis*
D052998:Microcystins