title: Membrane interaction of amphotericin B as single-length assembly examined by solid state NMR for uniformly 13C-enriched agent.
abstract: The membrane interaction of amphotericin B (AmB), one of the most important anti-fungal drugs, was investigated by solid state NMR measurements of uniformly 13C-enriched AmB, which was prepared by the culture of the drug-producing microorganism in the presence of [u-13C6]glucose. All the 13C NMR signals of AmB upon binding to DLPC membrane were successfully assigned on the basis of the 13C-13C correlation spectrum. 13C-31P RDX (Rotational-Echo Double Resonance for X-clusters) experiments clearly revealed the REDOR dephasing effects for carbon atoms residing in the both terminal parts, whereas no dephasing was observed for the middle parts including polyolefinic C20-C33 and hydroxyl-bearing C8/C9 parts. These observations suggest that AmB binds to DLPC membrane with a high affinity to the phospholipid and spans the membrane with a single molecular length.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, 1-16 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Matsuoka
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ikeuchi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Umegawa
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Matsumori
authors.forename: Michio
authors.initials: M
authors.lastname: Murata
mesh_terms: D000666:Amphotericin B / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002247:Carbon Isotopes; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D010713:Phosphatidylcholines / Q000737:chemistry; D013302:Streptomyces / Q000737:chemistry / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D002247:Carbon Isotopes; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; C015153:1,2-dilauroylphosphatidylcholine; D000666:Amphotericin B