[Study of fusion protein and attachment glycoprotein of Nipah virus expressed in recombinant baculovirus].
In this study, Recombinant baculoviruses rBac-NF and rBac-NG were generated for expressing F and G proteins Nipah virus (NiV) . The expression of recommbinant G (rNG) and F (rNF) protein in rBac-NF and rBac-NG infected cells were confirmed by western-blot. Both rNG and rNF showed sensitive and specific antigenic reaction to rabbit serum anti-Nipah virus in indirect immunofluorescence detection and indirect ELISA. Immunization with rBac-NF and rBac-NG infected insect cells elicited G ad F protein specific antibody responses in mice. Furthermore, the G ad F specific antibodies could neutralize the infectivity of the VSVdeltaG* F/G, the NiV F and G envelope glycoproteins psudotyped recombinant Vesicular Stomatitis Virus expressing green fluorescence protein. The results demonstrated F and G protein expressed by the recombinant baculoviruses could be safe economic diagnostic antigens for the surveillance and monitoring of NiV and promising subunit vaccines for the prevention of NiV.
Wang
Xi-Jun
XJ
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Hu
Sen
S
Ge
Jin-Ying
JY
Wang
Qing-Hua
QH
Qin
Li-Ting
LT
Bu
Zhi-Gao
ZG
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D045405:Nipah Virus / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; C510755:F protein, Nipah virus; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C116204:attachment protein G