Induction of abscisic acid-regulated gene expression by diacylglycerol pyrophosphate involves Ca2+ and anion currents in Arabidopsis suspension cells.
Diacylglycerol pyrophosphate (DGPP) was recently shown to be a possible intermediate in abscisic acid (ABA) signaling. In this study, reverse transcription-PCR of ABA up-regulated genes was used to evaluate the ability of DGPP to trigger gene expression in Arabidopsis (Arabidopsis thaliana) suspension cells. At5g06760, LTI30, RD29A, and RAB18 were stimulated by ABA and also specifically expressed in DGPP-treated cells. Use of the Ca2+ channel blockers fluspirilene and pimozide and the Ca2+ chelator EGTA showed that Ca2+ was required for ABA induction of DGPP formation. In addition, Ca2+ participated in DGPP induction of gene expression via stimulation of anion currents. Hence, a sequence of Ca2+, DGPP, and anion currents, constituting a core of early ABA-signaling events necessary for gene expression, is proposed.
Zalejski
Christine
C
Université Pierre et Marie Curie-Paris 6 and Centre National de la Recherche Scientifique, FRE 2846, Physiologie Cellulaire et Moléculaire des Plantes, F-94200 Ivry-sur-Seine, France.
Paradis
Sophie
S
Maldiney
Régis
R
Habricot
Yvette
Y
Miginiac
Emile
E
Rona
Jean-Pierre
JP
Jeannette
Emmanuelle
E
D000040:Abscisic Acid / Q000378:metabolism*; D000838:Anions / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D011756:Diphosphates / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D005484:Fluspirilene / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005990:Glycerol / Q000031:analogs & derivatives* / Q000378:metabolism; D008564:Membrane Potentials; D010868:Pimozide / Q000494:pharmacology; D015398:Signal Transduction
D000838:Anions; D029681:Arabidopsis Proteins; D002121:Calcium Channel Blockers; D002614:Chelating Agents; D011756:Diphosphates; C408642:diacylglycerol pyrophosphate; D010868:Pimozide; D004533:Egtazic Acid; D000040:Abscisic Acid; D005484:Fluspirilene; D005990:Glycerol; D002118:Calcium