Rats subjected to extended L-tryptophan restriction during early postnatal stage exhibit anxious-depressive features and structural changes.
Serotonin transmission dysfunction has been suggested to play an important role in depression and anxiety. This study reports the results of a series of experiments in which rats were subjected to extended maize-based tortilla diets during early postnatal stages. This diet contains only approximately 20% of the L-tryptophan in normal diets of laboratory rodents. Compared with controls, experimental rats displayed a significant increase of immobility counts in the forced swimming test and exhibited anxiety-like behavior in the elevated plus maze test after 1 month of diet treatment. Low levels of serotonin contents were found in prefrontal cortex, striatum, hippocampus, and brainstem using high-performance liquid chromatography. Immunocytochemical reactions against 5-Bromo-2'-deoxyuridine revealed a significant decrease in the proliferation rate for the subgranular zone of dentate gyrus. c-Fos expression after the forced swimming test was found reduced in prefrontal cortex, dentate gyrus, CA1, and hilus of hippocampus and amygdala. Moreover, dendrite arbor atrophy and decreased spine density were evident in Golgi-Cox-impregnated CA1 pyramidal neurons. Abnormal dendrite swelling in dentate gyrus granule cells was also observed. These findings indicate an involvement of hyposerotoninergia in emotional disturbance produced by L-tryptophan restriction during critical developmental stages and suggest that neuroplasticity changes might underlie these changes.
Zhang
Limei
L
Department Physiology, Faculty of Medicine, University of Mexico, Mexico City.
Guadarrama
Leyla
L
Corona-Morales
Aleph A
AA
Vega-Gonzalez
Arturo
A
Rocha
Luisa
L
Escobar
Alfonso
A
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001007:Anxiety / Q000503:physiopathology*; D001522:Behavior, Animal; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D003863:Depression / Q000503:physiopathology*; D018753:Diet, Protein-Restricted / Q000379:methods*; D004195:Disease Models, Animal; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018782:Maze Learning / Q000502:physiology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D016622:Silver Staining / Q000379:methods; D013550:Swimming; D014364:Tryptophan / Q000172:deficiency*; D014365:Tryptophan Hydroxylase / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D012701:Serotonin; D014364:Tryptophan; D014365:Tryptophan Hydroxylase; D001973:Bromodeoxyuridine