Polyelectrolyte complex formation mediated immobilization of chitosan-invertase neoglycoconjugate on pectin-coated chitin.
Saccharomyces cerevisiae invertase, chemically modified with chitosan, was immobilized on pectin-coated chitin support via polyelectrolyte complex formation. The yield of immobilized enzyme protein was determined as 85% and the immobilized biocatalyst retained 97% of the initial chitosan-invertase activity. The optimum temperature for invertase was increased by 10 degrees C and its thermostability was enhanced by about 10 degrees C after immobilization. The immobilized enzyme was stable against incubation in high ionic strength solutions and was 4-fold more resistant to thermal treatment at 65 degrees C than the native counterpart. The biocatalyst prepared retained 96 and 95% of the original catalytic activity after ten cycles of reuse and 74 h of continuous operational regime in a packed bed reactor, respectively.
Gómez
Leissy
L
Center for Enzyme Technology, University of Matanzas, 44740, Matanzas, Cuba.
Ramírez
Hector L
HL
Neira-Carrillo
Andrónico
A
Villalonga
Reynaldo
R
D019149:Bioreactors; D002686:Chitin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D007700:Kinetics; D008422:Materials Testing; D008567:Membranes, Artificial; D010368:Pectins / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D043324:beta-Fructofuranosidase / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D004800:Enzymes, Immobilized; D006023:Glycoproteins; D008567:Membranes, Artificial; D002686:Chitin; D010368:Pectins; D048271:Chitosan; D043324:beta-Fructofuranosidase