title: Biochemical study on the protective role of folic acid in rabbits treated with chromium (VI).
abstract: Deleterious effects of chromium (VI) compounds are diversified affecting almost all the organ systems in a wide variety of animals. Therefore, the present study was carried out to determine the effectiveness of folic acid (FA) in alleviating the toxicity of chromium (VI) on certain biochemical parameters, lipid peroxidation, and enzyme activities of male New Zealand white rabbits. Six rabbits per group were assigned to one of four treatment groups: 0 mg FA and 0 mg Cr(VI)/kg BW (control); 8.3 microg FA/kg BW; 5 mg Cr(VI)/kg BW; 5 mg Cr(VI) plus 8.3 microg FA/kg BW, respectively. Rabbits were orally administered their respective doses every day for 10 weeks. Results obtained showed that Cr(VI) significantly (P < 0.05) increased the levels of free radicals and the activity of glutathione S-transferase (GST), and decreased the content of sulfhydryl groups (SH groups) in liver, testes, brain, kidney, and lung. The activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AlP), acid phosphatase (AcP), and lactate dehydrogenase (LDH) were significantly decreased in liver and testes due to Cr(VI) administration. Also, AlP and AcP activities were significantly decreased in kidney and lung. The activity of acetylcholinesterase (AChE) was significantly decreased in brain and plasma. Contrariwise, the activities of AST and ALT were significantly increased in plasma, while AlP and AcP decreased. Chromium (VI) treatment caused a significant decrease in plasma total protein (TP) and globulin, and increased total lipids (TL), cholesterol, glucose, urea, creatinine, and bilirubin concentrations. Folic acid alone significantly decreased the levels of free radicals in liver, brain, and kidney, and increased the content of SH-group. The activities of AST, ALT, and LDH in liver; AST, ALT, AlP, AcP, and LDH in testes; AcP in kidney; AlP and AcP in lung, and LDH in brain were significantly increased. Plasma TP and albumin were increased, while urea and creatinine were decreased. The presence of FA with Cr(VI) restored the changes in enzyme activities and biochemical parameters. In conclusion, folic acid could be effective in the protection of chromium-induced toxicity.
authors.affiliation: Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Egypt. feldemerdash@yahoo.com
authors.forename: Fatma M
authors.initials: FM
authors.lastname: El-Demerdash
authors.forename: Mokhtar I
authors.initials: MI
authors.lastname: Yousef
authors.forename: Fathia A M
authors.initials: FA
authors.lastname: Elaswad
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001681:Biological Assay; D001921:Brain / Q000201:enzymology; D002857:Chromium / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005492:Folic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D005609:Free Radicals / Q000032:analysis; D007668:Kidney / Q000201:enzymology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011817:Rabbits; D013737:Testis / Q000201:enzymology; D014131:Trace Elements / Q000633:toxicity*; D014803:Vitamin B Complex / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D005609:Free Radicals; D014131:Trace Elements; D002857:Chromium; D014803:Vitamin B Complex; D005492:Folic Acid