title: Bbt-TNFR1 and Bbt-TNFR2, two tumor necrosis factor receptors from Chinese amphioxus involve in host defense.
abstract: Two novel tumor necrosis factor receptors, Bbt-TNFR1 and Bbt-TNFR2, were isolated from Chinese amphioxus, the closest relative to vertebrate. The mRNA of Bbt-TNFR1 encoded a type I membrane protein of 452 amino acids, including four cysteine-rich domains in the extracellular region and a putative TRAF6-binding site at its 154aa long cytoplasmic tail. Bbt-TNFR2 was a 304aa long type I membrane protein, featuring three cysteine-rich domains and a short cytoplasmic tail of just 13 amino acids. Southern blot revealed that Bbt-TNFR1 was a single copy gene, while Bbt-TNFR2 was presented in multiple copies. Sequence comparison indicated that both Bbt-TNFR1 and Bbt-TNFR2 were weakly similar to LT-bR, HVEM, TNFR2, CD40, OX40 and DcR3. Real-time PCR showed that Bbt-TNFR1 and Bbt-TNFR2 were regulated during development and finally had high expression in mucosa-rich tissues in adult stage. Furthermore, up-regulated expression of both genes was also observed in guts after Gram-positive bacteria challenge. However, not like Bbt-TNFR2's slowly and gradually augmentation in the following 48 h, expression of Bbt-TNFR1 dramatically surged up within 4 h and then subsided rapidly. Taking together, Bbt-TNFR1 and Bbt-TNFR2 may involve in the host defense of Chinese amphioxus via distinct fashions.
authors.affiliation: Department of Biochemistry, Open Laboratory for Marine Functional Genomics of State High-Tech Development Program, Guangdong Key Laboratory of Therapeutic Functional Genes, College of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou, PR China.
authors.forename: Shaochun
authors.initials: S
authors.lastname: Yuan
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Yu
authors.forename: Shengfeng
authors.initials: S
authors.lastname: Huang
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Meiling
authors.initials: M
authors.lastname: Dong
authors.forename: Shangwu
authors.initials: S
authors.lastname: Chen
authors.forename: Yingcai
authors.initials: Y
authors.lastname: Yu
authors.forename: Anlong
authors.initials: A
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002816:Chordata, Nonvertebrate / Q000235:genetics* / Q000276:immunology; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics* / Q000276:immunology; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000235:genetics* / Q000276:immunology; D013203:Staphylococcal Infections / Q000276:immunology; D013211:Staphylococcus aureus
chemical_list: D012333:RNA, Messenger; D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II