title: The mouse immune response to carrier erythrocyte entrapped antigens.
abstract: This study investigated the potential of a single administration of carrier erythrocyte entrapped antigen to elicit humoral responses in the Balb/c mouse. Humoral responses to primary immunizations of erythrocyte encapsulated antigens were compared with those obtained with adjuvanted antigen administered via the subcutaneous route. Ig isotype responses to primary immunizations of erythrocyte entrapped antigen and subcutaneous antigen were compared to responses observed in mice that subsequently received booster immunizations with un-entrapped antigen. This study demonstrates that a single administration of antigen-loaded carrier erythrocytes is able to elicit humoral immune responses comparable or superior to those obtained via the adjuvanted subcutaneous vaccination route. The IgG isotype profiles demonstrate that the erythrocyte entrapment of antigens is another mechanism by which the Th responses to antigens maybe modulated.
authors.affiliation: Child Health, Division of Clinical Developmental Sciences, St. George's University of London, Cranmer Terrace, London SW17 0RE, United Kingdom.
authors.forename: Anne M
authors.initials: AM
authors.lastname: Murray
authors.forename: Ian F S
authors.initials: IF
authors.lastname: Pearson
authors.forename: Lynette D
authors.initials: LD
authors.lastname: Fairbanks
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Chalmers
authors.forename: Murray D
authors.initials: MD
authors.lastname: Bain
authors.forename: Bridget E
authors.initials: BE
authors.lastname: Bax
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000502:physiology*; D000941:Antigens / Q000008:administration & dosage*; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D007114:Immunization / Q000379:methods*; D007074:Immunoglobulin G / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000941:Antigens; D007074:Immunoglobulin G