[Effect of vitexia-rhamnoside (V-R) on vasomotor factors expression of endothelial cell].
OBJECTIVE
To observe the influence of vitexia-rhamnoside (V-R) on vasomotor factor expression of endothelial cell (EC) damaged by hypoxia and reoxygenation.


METHODS
The cultured human umbilical vein endothelial cells (HUV ECs) were subject to ischemia and reperfusion following hypoxia and reoxygenation. The levels of ET-1, NO and NOS intracellular in culture supertanants were measured by radioimmunity, Griess and immunohistochemistry, respectively. And the gene expressions of ET-1 and NOS intracellular were measured by reverse transcriptase-polymerase chain reaction.


RESULTS
V-R at different doses markedly increased the gene expression and activity of NOS, enhanced the level of vaso-dilating factor NO, and significantly decreased the gene expression and production of vaso-constricting factor ET-1 of EC.


CONCLUSIONS
We have demonstrated that V-R had the regulatory effect on the expression of vaso-active substances of EC damaged by hypoxia and reoxygenation in the levels of protein and gene transcription of cytokines.
Zhu
Xiao-Xin
XX
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. zhuxiaoxin@mail.cintcm.com.cn
Li
Lian-Da
LD
Liu
Jian-Xun
JX
Liu
Zhi-Yun
ZY
Ma
Xue-Ying
XY
D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D027825:Crataegus / Q000737:chemistry; D042783:Endothelial Cells / Q000378:metabolism*; D019332:Endothelin-1 / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis / Q000235:genetics; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology
D019332:Endothelin-1; D012333:RNA, Messenger; D009569:Nitric Oxide; C021329:vitexin rhamnoside; D047310:Apigenin; D019001:Nitric Oxide Synthase