Molecular cloning and characterization of a rice blast-inducible RING-H2 type zinc finger gene.
A novel blast-inducible RING-H2 type zinc finger protein gene OsRING-1 was cloned from rice by cDNA library screening. OsRING-1 is 1670 bp in length and encodes a 46.6 kDa basic protein with two transmembrane (TM) domains, a basic domain (BD), a conserved domain (CD), a RING finger domain and a serine rich (S-rich) domain. By database search, OsRING-1 was mapped on chromosome 2 and clustered together with other six zinc finger genes. The promoter sequence analysis of OsRING-1 gene revealed that some ABA, GA, ethylene, wound, drought, heat stress and pathogen infection responsive elements were found within the OsRING-1 promoter region. Northern analysis showed that OsRING-1 was induced in different degree by pathogen infections, SA, ABA, JA and ethephon (ET) treatments. Tissue expression analysis showed that OsRING-1 was constitutively strongly expressed in roots, but faintly in stems, leaves and sheaths. Taken together, OsRING-1, as a novel C3H2C3-type zinc finger protein involved in many stress responses in rice might plays a role as a transcription regulator in plant stress response signal transduction pathways.
Meng
Xiang-Bing
XB
Department of Plant Pathology, China Agricultural University, Beijing 100094, People's Republic of China.
Zhao
Wen-Sheng
WS
Lin
Rui-Ming
RM
Wang
Min
M
Peng
You-Liang
YL
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020082:Magnaporthe / Q000472:pathogenicity; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D016415:Sequence Alignment; D017421:Sequence Analysis; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant