title: Production of Trichoderma strains with pesticide-polyresistance by mutagenesis and protoplast fusion.
abstract: The sensitivity of two cold-tolerant Trichoderma strains belonging to the species T. harzianum and T. atroviride was determined to a series of pesticides widely used in agriculture. From the 16 pesticides tested, seven fungicides: copper sulfate, carbendazim, mancozeb, tebuconazole, imazalil, captan and thiram inhibited colony growth of the test strains significantly with minimal inhibitory concentrations of 300, 0.4, 50, 100, 100, 100 and 50 microg/ml, respectively. Mutants resistant to carbendazim and tebuconazole were produced from both wild type strains by means of UV-mutagenesis. The cross-resistance capabilities and in vitro antagonistic properties of the mutants were determined. Carbendazim-resistant mutants showed total cross-resistance to benomyl and thiabendazole at a concentration of 20 microg/ml. Intraspecific protoplast fusion was carried out between carbendazim- and tebuconazole-resistant mutants of both parental strains, and putative haploid recombinants with stable resistance to both pesticides were produced in the case of T. atroviride. These pesticide-polyresistant progenies are potential candidates for application in an integrated pest management system.
authors.affiliation: Department of Microbiology, University of Szeged, PO Box 533, H-6701, Szeged, Hungary. lori@szegedkendo.hu
authors.forename: Lóránt
authors.initials: L
authors.lastname: Hatvani
authors.forename: László
authors.initials: L
authors.lastname: Manczinger
authors.forename: László
authors.initials: L
authors.lastname: Kredics
authors.forename: András
authors.initials: A
authors.lastname: Szekeres
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Antal
authors.forename: Csaba
authors.initials: C
authors.lastname: Vágvölgyi
mesh_terms: D000898:Antibiosis; D001542:Benomyl / Q000494:pharmacology; D025141:Drug Resistance, Fungal; D005658:Fungi / Q000254:growth & development; D005659:Fungicides, Industrial / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D016296:Mutagenesis; D010575:Pesticides / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology; D011523:Protoplasts; D014230:Triazoles / Q000494:pharmacology; D014242:Trichoderma / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D014466:Ultraviolet Rays
chemical_list: D005659:Fungicides, Industrial; D010575:Pesticides; D014230:Triazoles; C087114:tebuconazole; D001542:Benomyl