title: New approaches in the modulation of bladder smooth muscle cells on viable detrusor constructs.
abstract: Identification of biochemical and mechanical stimuli in order to modulate the function of bladder smooth muscle cells (SMC) in viable detrusor constructs. Human bladder detrusor cells were seeded on bladder acellular matrix and cultured under different conditions. Cell viability and proliferation were assessed by fluorescent microscopic analyses. Histological, immunohistochemical and flow cytometric analyses were performed to compare growth characteristics and differentiation of SMC. The combination of medium conditioned with proliferative urothelium and mechanical stretch resulted in a more densely populated membrane. In this culture system, the expression of alpha-smooth muscle actin (alpha-SMA) and desmin were clearly induced after serum elimination. SMC-phenotype can be modulated in viable detrusor constructs by applying selected combinations of urothelial-conditioned media and mechanical stimulation under stepwise reduction and elimination of serum.
authors.affiliation: Department of Urology, Technical University of Dresden, Fetscherstrasse 74, 01307, Dresden, Germany. Gouya.Ram-Liebig@uniklinikum-dresden.de
authors.forename: Gouya
authors.initials: G
authors.lastname: Ram-Liebig
authors.forename: Ursula
authors.initials: U
authors.lastname: Ravens
authors.forename: Bartosz
authors.initials: B
authors.lastname: Balana
authors.forename: Michael
authors.initials: M
authors.lastname: Haase
authors.forename: Gustavo
authors.initials: G
authors.lastname: Baretton
authors.forename: Manfred P
authors.initials: MP
authors.lastname: Wirth
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D003584:Cytological Techniques; D003893:Desmin / Q000032:analysis; D005434:Flow Cytometry; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D013552:Swine; D001743:Urinary Bladder / Q000166:cytology*
chemical_list: D000199:Actins; D003893:Desmin