CP110 cooperates with two calcium-binding proteins to regulate cytokinesis and genome stability.
The centrosome is an integral component of the eukaryotic cell cycle machinery, yet very few centrosomal proteins have been fully characterized to date. We have undertaken a series of biochemical and RNA interference (RNAi) studies to elucidate a role for CP110 in the centrosome cycle. Using a combination of yeast two-hybrid screens and biochemical analyses, we report that CP110 interacts with two different Ca2+-binding proteins, calmodulin (CaM) and centrin, in vivo. In vitro binding experiments reveal a direct, robust interaction between CP110 and CaM and the existence of multiple high-affinity CaM-binding domains in CP110. Native CP110 exists in large (approximately 300 kDa to 3 MDa) complexes that contain both centrin and CaM. We investigated a role for CP110 in CaM-mediated events using RNAi and show that its depletion leads to a failure at a late stage of cytokinesis and the formation of binucleate cells, mirroring the defects resulting from ablation of either CaM or centrin function. Importantly, expression of a CP110 mutant unable to bind CaM also promotes cytokinesis failure and binucleate cell formation. Taken together, our data demonstrate a functional role for CaM binding to CP110 and suggest that CP110 cooperates with CaM and centrin to regulate progression through cytokinesis.
Tsang
William Y
WY
Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.
Spektor
Alexander
A
Luciano
Daniel J
DJ
Indjeian
Vahan B
VB
Chen
Zhihong
Z
Salisbury
Jeffery L
JL
Sánchez
Irma
I
Dynlacht
Brian David
BD
D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D048749:Cytokinesis; D015870:Gene Expression; D042822:Genomic Instability; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000172:deficiency / Q000378:metabolism*; D008970:Molecular Weight; D046912:Multiprotein Complexes / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010750:Phosphoproteins / Q000172:deficiency / Q000378:metabolism*; D011123:Polyploidy; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D034622:RNA Interference
C467559:CCP110 protein, human; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes; D010750:Phosphoproteins