Value of the chlorhexidine decontamination method for recovery of nontuberculous mycobacteria from sputum samples of patients with cystic fibrosis.
The chlorhexidine method was compared to the N-acetyl-l-cysteine-NaOH-oxalic acid decontamination method currently recommended for the recovery of nontuberculous mycobacteria (NTM) from patients with cystic fibrosis. Sputum samples (n = 827) treated with chlorhexidine yielded twice as many NTM-positive cultures as those treated by the reference method (54 [6.50%] versus 27 [3.25%]; P < 0.0001) despite a higher contamination rate (20% versus 14.2%; P = 0.0017).
Ferroni
Agnès
A
Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France. agnes.ferroni@nck.aphp.fr
Vu-Thien
Hoang
H
Lanotte
Philippe
P
Le Bourgeois
Muriel
M
Sermet-Gaudelus
Isabelle
I
Fauroux
Brigitte
B
Marchand
Sophie
S
Varaigne
Françoise
F
Berche
Patrick
P
Gaillard
Jean-Louis
JL
Offredo
Catherine
C
D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D002710:Chlorhexidine / Q000494:pharmacology*; D003550:Cystic Fibrosis / Q000382:microbiology*; D003666:Decontamination / Q000379:methods*; D006801:Humans; D009161:Mycobacterium / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000382:microbiology; D019815:Oxalic Acid / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology; D013183:Sputum / Q000382:microbiology*
D012972:Sodium Hydroxide; D019815:Oxalic Acid; D002710:Chlorhexidine; D000111:Acetylcysteine