ADAP is dispensable for NK cell development and function.
NK cells are key mediators of the innate immune response and anti-tumor surveillance. Adhesion and degranulation-promoting adapter protein (ADAP, formerly known as SLAP-130 or Fyb) is a hematopoietic-specific adapter that is required for efficient TCR signaling and T cell activation. Herein, we examine a potential role for ADAP in NK development and function. ADAP is expressed in primary NK cells and in IL-2 stimulated lymphokine-activated killers. However, ADAP-deficient mice show no defects in NK development. Further, ADAP is dispensable for key NK functions, including cytotoxicity in response to engagement of activating receptors, cytokine production, conjugate formation and tumor suppression in vivo. These results indicate that, unlike events stimulated by TCR engagement, signaling events engaged by immunoreceptor tyrosine-based activation motif-associated and cytokine receptors on NK cells can occur independently of ADAP.
Fostel
Lindsey V
LV
Department of Internal Medicine, University of Minnesota Medical School, Center for Immunology, Cancer Center, University of Minnesota, Minneapolis, 55455, USA.
Dluzniewska
Joanna
J
Shimizu
Yoji
Y
Burbach
Brandon J
BJ
Peterson
Erik J
EJ
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology; D000950:Antigens, Ly / Q000276:immunology / Q000378:metabolism; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology / Q000276:immunology; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D055630:Receptors, NK Cell Lectin-Like
D048868:Adaptor Proteins, Signal Transducing; D000950:Antigens, Ly; C491468:Fyb protein, mouse; D007376:Interleukin-2; D037181:Lectins, C-Type; D055630:Receptors, NK Cell Lectin-Like; D018664:Interleukin-12; D007371:Interferon-gamma