Characterization of two epimers, 4alpha and 4beta, of a novel podophyllotoxin-4-O-(D)-6-acetylglucopyranoside from Podophyllum hexandrum by LC-ESI-MS-MS.
High-performance liquid chromatography (HPLC) with diode array detection interfaced to electrospray ionization (ESI) mass spectrometry (MS) is applied to identify the two epimers of a novel and minor constituent, podophyllotoxin-4-O-(D)-6-acetylglucopyraniside from high-altitude Podophyllum hexandrum for the first time. This is done by matching the structural information from the tandem MS data with the reported lignan markers. The results show that LC-MS-MS is the method of choice for fast detection and detailed chemical analysis of mixtures in the crude extracts of Podophyllum. The method can be employed in the absence of reference standards for the markers and is particularly useful in view of the scarcity of these rare chemical standards.
Puri
S C
SC
Division of Natural Products Chemistry, Regional Research Laboratory (CSIR), Jammu-180001, India. purisc@yahoo.com
Handa
G
G
Bhat
B A
BA
Dhar
K L
KL
Spiteller
Michael
M
Qazi
G N
GN
D002853:Chromatography, Liquid / Q000379:methods*; D005960:Glucosides / Q000737:chemistry*; D011034:Podophyllotoxin / Q000031:analogs & derivatives* / Q000737:chemistry; D011035:Podophyllum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D005960:Glucosides; C430086:podophyllotoxin 4-O-glucopyranoside; D011034:Podophyllotoxin