[Detecting the DNA polymorphism of human complement component C8A by PCR-SSCP analysis].
OBJECTIVE
The aim of this study is to establish a new method for detecting the DNA polymorphism of human complement component C8A.


METHODS
Based on the point mutation (C-->A) of C8A gene in exon3,The genotypes of 98 unrelated individuals from Han population in Chengdu were studied by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP)analysis followed by DNA sequencing.


RESULTS
The genotypes of C8A * AA, C8A * BB and C8A * AB were observed and the distribution of the genotypes frequencies of C8A in Chengdu Han population was in accordance with Hardy-Weinberg equilibrium.


CONCLUSIONS
The method of PCR-SSCP is reliable, rapid, simple and cost-effective in detecting the DNA polymorphism of C8A, and it is valuable for further application in population genetic study and forensic science practice.
Yang
Zhi-hui
ZH
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Zhang
Lin
L
Zhou
Bin
B
Wang
Chuang
C
Jia
Jing
J
D000328:Adult; D001483:Base Sequence; D003185:Complement C8 / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
D003185:Complement C8; D004247:DNA