Kidney transplantation procedures in rats: assessments, complications, and management.
Kidney transplantation in rats is an experimental model often used for the development of general microsurgical or transplantation techniques, for immunologic studies, and for analyzing transplant-associated long-term arterial blood-pressure changes. The aim of the present study was to analyze different surgical techniques of kidney transplantation in rats, with emphasis on minimizing surgical complications and establishing guidelines for their prevention and management. Complications were categorized into general (e.g., core body temperature drop, ischemic time) and surgically related vascular and urinary tract complications. In conclusion, a significant reduction of the complication rate in renal transplantation in rats can be achieved by placing the animal on a heating pad at an appropriate temperature. To reduce the risk of vascular thrombosis, ice-cold saline with heparin and careful flushing of the donor kidneys are recommended. Vascular complications can be avoided by performing "end-to-end" anastomosis techniques. The use of stents and cannulas in the urinary tract is associated with a high risk of urinary tract obstruction, and therefore is not recommended.
Pahlavan
Payam S
PS
Department of Physiology and Pathophysiology, University of Heidelberg, Heidelberg, Germany. payam.pahlavan@physiologie.uni-heidelberg.de
Smallegange
Corry
C
Adams
Michael A
MA
Schumacher
Martin
M
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D016030:Kidney Transplantation / Q000379:methods*; D023421:Models, Animal; D011183:Postoperative Complications / Q000517:prevention & control*; D017410:Practice Guidelines as Topic; D051381:Rats; D014513:Ureter / Q000601:surgery; D014656:Vascular Surgical Procedures / Q000379:methods