Chitosan scaffolds for in vitro buffalo embryonic stem-like cell culture: an approach to tissue engineering.
Three-dimensional (3D) porous chitosan scaffolds are attractive candidates for tissue engineering applications. Chitosan scaffolds of 70, 88, and 95% degree of deacetylation (% DD) with the same molecular weight were developed and their properties with buffalo embryonic stem-like (ES-like) cells were investigated in vitro. Scaffolds were fabricated by freezing and lyophilization. They showed open pore structure with interconnecting pores under scanning electron microscopy (SEM). Higher % DD chitosan scaffolds had greater mechanical strength, slower degradation rate, lower water uptake ability, but similar water retention ability, when compared to lower % DD chitosan. As a strategy to tissue engineering, buffalo ES-like cells were cultured on scaffolds for 28 days. It appeared that chitosan was cytocompatible and cells proliferated well on 88 and 95% DD scaffolds. In addition, the buffalo ES-like cells maintained their pluripotency during the culture period. Furthermore, the SEM and histological study showed that the polygonal buffalo ES-like cells proliferated well and attached to the pores. This study proved that 3D biodegradable highly deacetylated chitosan scaffolds are promising candidates for ES-like cell based tissue engineering and this chitosan scaffold and ES cell based system can be used as in vitro model for subsequent clinical applications.
Thein-Han
Wah W
WW
Institute of Science and Technology for Research and Development, Mahidol University, Salaya Campus, Nakhorn Pathom 73170, Thailand.
Kitiyanant
Yindee
Y
D000818:Animals; D001672:Biocompatible Materials; D002020:Buffaloes; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan; D053595:Embryonic Stem Cells / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D039904:Pluripotent Stem Cells / Q000648:ultrastructure; D023822:Tissue Engineering
D001672:Biocompatible Materials; D048271:Chitosan