Determination of genomic damage in neuroblastic tumors by arbitrarily primed PCR: MYCN amplification as a marker for genomic instability in neuroblastomas.
The aim of this study is to establish an estimation of the global genomic alteration in neuroblastic tumors (ganglioneuromas, ganglioneuroblastomas and neuroblastomas) and correlate them with different clinical parameters (age, sex, diagnosis, Shimada index, proliferation index, tumor location, and 1p and v-myc avian myelocitomatosis viral-related (MYCN) status) in order to find new molecular and/or prognostic markers for neuroblastoma. To assess the genomic damage in neuroblastic tumors, we used an arbitrarily primed PCR approach, a technique based on the reproducibility of band profiles obtained by a PCR with a low annealing temperature in its first cycles. Genomic damage was assessed by comparing band profiles of tumors and normal paired samples. Gains and losses in the intensity of the bands were computerized and referred to the total number of bands analyzed. We found a higher genomic damage fraction (GDF) in the female's group (U-Mann-Whitney, P = 0.025), but we could not find any association between GDF and tumor location, proliferation index, diagnosis or age of the patient. There was no relationship between 1p status and GDF, but tumors with MYCN amplification had a slightly higher GDF. MYCN amplification might in some way contribute to genomic instability of neuroblastomas.
Muñoz
Jorge
J
Molecular Neuro-Oncology Laboratory, University of Navarra, Pamplona, Spain.
Vendrell
Elisenda
E
Aiza
Gemma
G
Nistal
Manuel
M
Pestaña
Angel
A
Peinado
Miguel Angel
MA
Castresana
Javier S
JS
D002675:Child, Preschool; D002869:Chromosome Aberrations; D005260:Female; D005729:Ganglioneuroma / Q000235:genetics* / Q000473:pathology; D005784:Gene Amplification; D016259:Genes, myc; D005819:Genetic Markers; D042822:Genomic Instability; D006801:Humans; D007223:Infant; D007680:Kidney Neoplasms / Q000235:genetics / Q000473:pathology; D019656:Loss of Heterozygosity; D008297:Male; D008479:Mediastinal Neoplasms / Q000235:genetics / Q000473:pathology; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D012186:Retroperitoneal Neoplasms / Q000235:genetics / Q000473:pathology; D012737:Sex Factors
D005819:Genetic Markers