title: [Effect of vitexia-rhamnoside (V-R) on vasomotor factors expression of endothelial cell].
abstract: OBJECTIVE
  To observe the influence of vitexia-rhamnoside (V-R) on vasomotor factor expression of endothelial cell (EC) damaged by hypoxia and reoxygenation.
  
  
  METHODS
  The cultured human umbilical vein endothelial cells (HUV ECs) were subject to ischemia and reperfusion following hypoxia and reoxygenation. The levels of ET-1, NO and NOS intracellular in culture supertanants were measured by radioimmunity, Griess and immunohistochemistry, respectively. And the gene expressions of ET-1 and NOS intracellular were measured by reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  V-R at different doses markedly increased the gene expression and activity of NOS, enhanced the level of vaso-dilating factor NO, and significantly decreased the gene expression and production of vaso-constricting factor ET-1 of EC.
  
  
  CONCLUSIONS
  We have demonstrated that V-R had the regulatory effect on the expression of vaso-active substances of EC damaged by hypoxia and reoxygenation in the levels of protein and gene transcription of cytokines.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. zhuxiaoxin@mail.cintcm.com.cn
authors.forename: Xiao-Xin
authors.initials: XX
authors.lastname: Zhu
authors.forename: Lian-Da
authors.initials: LD
authors.lastname: Li
authors.forename: Jian-Xun
authors.initials: JX
authors.lastname: Liu
authors.forename: Zhi-Yun
authors.initials: ZY
authors.lastname: Liu
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Ma
mesh_terms: D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D027825:Crataegus / Q000737:chemistry; D042783:Endothelial Cells / Q000378:metabolism*; D019332:Endothelin-1 / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis / Q000235:genetics; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D019332:Endothelin-1; D012333:RNA, Messenger; D009569:Nitric Oxide; C021329:vitexin rhamnoside; D047310:Apigenin; D019001:Nitric Oxide Synthase