Comparing the Multi-ZSC one-step standardized swim-up method to the double-wash swim-up method with regard to the effects of sperm separation on morphology, head morphometry, and acrosome reaction inducibility.
The objective of this study was to test the efficacy of the new Multi-ZSC system on human sperm separation compared with the commonly used double-wash swim-up method. It was found that the double-wash swim-up method selects sperm with better head morphometry and acrosome reaction inducibility than the upper fractions of the Multi-ZSC.
Lampiao
Fanuel
F
Department of Medical Physiology and Biochemistry, Faculty of Health Sciences, University of Stellenbosch, Tygerberg, South Africa.
du Plessis
Stefan S
SS
D018929:Cell Culture Techniques / Q000379:methods* / Q000592:standards; D002469:Cell Separation / Q000379:methods* / Q000592:standards; D006801:Humans; D008297:Male; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D013076:Sperm Count / Q000379:methods*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*