title: Comparing the Multi-ZSC one-step standardized swim-up method to the double-wash swim-up method with regard to the effects of sperm separation on morphology, head morphometry, and acrosome reaction inducibility.
abstract: The objective of this study was to test the efficacy of the new Multi-ZSC system on human sperm separation compared with the commonly used double-wash swim-up method. It was found that the double-wash swim-up method selects sperm with better head morphometry and acrosome reaction inducibility than the upper fractions of the Multi-ZSC.
authors.affiliation: Department of Medical Physiology and Biochemistry, Faculty of Health Sciences, University of Stellenbosch, Tygerberg, South Africa.
authors.forename: Fanuel
authors.initials: F
authors.lastname: Lampiao
authors.forename: Stefan S
authors.initials: SS
authors.lastname: du Plessis
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods* / Q000592:standards; D002469:Cell Separation / Q000379:methods* / Q000592:standards; D006801:Humans; D008297:Male; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D013076:Sperm Count / Q000379:methods*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*