Decolorization and removal of textile and non-textile dyes from polluted wastewater and dyeing effluent by using potato (Solanum tuberosum) soluble and immobilized polyphenol oxidase.
Celite bound potato polyphenol oxidase preparation was employed for the treatment of wastewater/dye effluent contaminated with reactive textile and non-textile dyes, Reactive Blue 4 and Reactive Orange 86. The maximum decolorization was found at pH 3.0 and 4.0 in case of Reactive Blue 4 and Reactive Orange 86, respectively. Immobilized potato polyphenol oxidase was significantly more effective in decolorizing the individual dye and complex mixtures of dyes as compared to soluble enzyme. The absorption spectra of the treated and untreated dye mixture and dyeing effluent exhibited a marked difference in the absorption value at various wavelengths. The polluted water contaminated with an individual dye or mixtures of dyes treated with soluble and immobilized potato polyphenol oxidase resulted in the remarkable loss in total organic carbon.
Khan
Amjad Ali
AA
Department of Biochemistry, Faculty of Life Science, Aligarh Muslim University, Aligarh 202 002, UP, India.
Husain
Qayyum
Q
D001673:Biodegradation, Environmental; D002244:Carbon / Q000032:analysis; D004156:Catechol Oxidase / Q000378:metabolism*; D003116:Color; D004396:Coloring Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007220:Industrial Waste; D011198:Solanum tuberosum / Q000201:enzymology*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013783:Textile Industry; D013784:Textiles; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D018508:Water Purification / Q000379:methods*
D004396:Coloring Agents; D004800:Enzymes, Immobilized; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D002244:Carbon; D004156:Catechol Oxidase