Induction of differentiation in leukemic cell strains with myelopeptide-4.
We studied the capacity of myelopeptide-4 (regulatory peptide of the bone marrow origin) to induce terminal differentiation of HL-60 and K-562 leukemic cells. Myelopeptide-4 increased the expression of CD14 and CD38 differentiation antigens on the surface of HL-60 cells and of CD44 antigen on K-562 cells, induced the appearance of mature monocyte/macrophages in HL-60 culture and hemoglobin-producing cells in K-562 cell culture, and stimulated phagocytic activity of THP-1 leukemic cells. Myelopeptide-4 is an endogenous factor of cell differentiation, a prospective agent for antileukemic therapy.
Kirilina
E A
EA
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Suvorov
N I
NI
Popova
S S
SS
Khaidukov
S V
SV
Rapoport
E M
EM
Fonina
L A
LA
Mikhailova
A A
AA
D051997:ADP-ribosyl Cyclase 1 / Q000096:biosynthesis; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D018922:HL-60 Cells; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D020014:K562 Cells; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology*; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D009842:Oligopeptides / Q000378:metabolism*; D010587:Phagocytosis
D006454:Hemoglobins; D018960:Hyaluronan Receptors; D018950:Lipopolysaccharide Receptors; D009842:Oligopeptides; C502954:phenylalanyl-arginyl-prolyl-arginyl-isoleucyl-methionyl-threonyl-proline; D051997:ADP-ribosyl Cyclase 1