title: Inactivation of nanocrystalline C60 cytotoxicity by gamma-irradiation.
abstract: We investigated the effect of gamma-irradiation on the cytotoxicity of pure C60 solubilized in water by using tetrahydrofuran (THF/n-C60 or THF/n-C60). In contrast to THF/n-C60, its gamma-irradiated counterpart failed to generate oxygen radicals and cause extracellular signal-regulated kinase (ERK)-dependent necrotic cell death in various types of mammalian cells. Moreover, gamma-irradiated THF/n-C60 protected cells from the oxidative stress induced by native THF/n-C60 or hydrogen peroxide. The observed biological effects were associated with gamma-irradiation-mediated decomposition of THF and subsequent derivatization of the n-C60 surface. These results for the first time demonstrate gamma-irradiation-mediated changes in the physico-chemical properties of THF-prepared nanocrystalline C60, resulting in a complete loss of its cytotoxic effect and its conversion to a cytoprotective agent.
authors.affiliation: Institute of Biochemistry, School of Medicine, 11000 Belgrade, Serbia and Montenegro.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Isakovic
authors.forename: Zoran
authors.initials: Z
authors.lastname: Markovic
authors.forename: Nadezda
authors.initials: N
authors.lastname: Nikolic
authors.forename: Biljana
authors.initials: B
authors.lastname: Todorovic-Markovic
authors.forename: Sanja
authors.initials: S
authors.lastname: Vranjes-Djuric
authors.forename: Ljubica
authors.initials: L
authors.lastname: Harhaji
authors.forename: Nevena
authors.initials: N
authors.lastname: Raicevic
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Romcevic
authors.forename: Dana
authors.initials: D
authors.lastname: Vasiljevic-Radovic
authors.forename: Miroslav
authors.initials: M
authors.lastname: Dramicanin
authors.forename: Vladimir
authors.initials: V
authors.lastname: Trajkovic
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D037741:Fullerenes / Q000528:radiation effects / Q000633:toxicity*; D005663:Furans / Q000528:radiation effects / Q000633:toxicity; D005720:Gamma Rays; D051379:Mice; D049329:Nanostructures
chemical_list: D037741:Fullerenes; D005663:Furans; C018674:tetrahydrofuran; C069837:fullerene C60