Secondary metabolites from Opuntia ficus-indica var. saboten.
A butanol fraction, from the methanolic extract of Opuntia ficus-indica var. saboten, on purification either by preparative TLC or reversed phase HPLC, yielded three chemical components: isorhamnetin 3-O-(6''-O-E-feruloyl)neohesperidoside (1), (6R)-9,10-dihydroxy-4,7-megastigmadien-3-one-9-O-beta-D-glucopyranoside (2) and (6S)-9,10-dihydroxy-4,7-megastigmadien-3-one-9-O-beta-D-glucopyranoside (3) along with 15 known compounds. Structures of compounds (1-3) were elucidated by aid of spectroscopic analyses. The absolute stereochemistry in compounds 2 and 3 was established with the help of CD data analysis and comparison with the literature data. In a DPPH radical scavenging assay, compound 1 showed moderate inhibitory activity (IC50 = 45.58 microg/ml).
Saleem
Muhammad
M
Division of Life Sciences, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, Republic of Korea.
Kim
Hyoung Ja
HJ
Han
Chang Kyun
CK
Jin
Changbae
C
Lee
Yong Sup
YS
D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D029747:Opuntia / Q000737:chemistry* / Q000378:metabolism*; D013237:Stereoisomerism