A high-performance liquid chromatography method for determination 2-(n-(N,N,N-trimethyl)-n-alkyl)-5-alkylfuryl halides in dipalmitoylphosphatidilcholine liposome solutions.
A high-performance liquid chromatography (HPLC) method for the determination of 2-(4-(N,N,N-trimethyl)-butyl)-5-dodecylfuryl bromide (DFTA) in dipalmitoylphophatidil-choline (DPPC) liposome solutions has been developed. Lipid-soluble furan derivatives, 2,5-disubstituted with different n-alkyl chains and a terminal trimethylammonium group are useful probes for studying singlet oxygen dynamics and equilibria in microcompartmentalized systems. The actual HPLC method uses a gradient elution and DAD detection. The chromatographic separation of these components is achieved using a C18 analytical column with a 10mM solution of 1-heptanesulfonic acid (PIC-7)-methanol (10:90, v/v) as initial mobile phase. Both DFTA peaks are well resolved and free of interference from matrix components and reaction products. The method has been found to be linear (r > 0.999) over a wide concentration range and reliable to perform kinetic experiments in which the time dependent consumption of a tetraalkylammonium surfactant in a microorganized systems composed by lipidic surfactants is followed.
Morales
Javier
J
Universidad de Chile, Facultad de Ciencias Químicas y Farmacéuticas, Departamento de Química Orgánica y Fisicoquímica, Olivos 1007, Casilla 233, Santiago, Chile.
Zanocco
Antonio L
AL
Günther
Germán
G
Lemp
Else
E
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry* / Q000592:standards; D000473:Alkanes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000737:chemistry; D005663:Furans / Q000737:chemistry*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D015203:Reproducibility of Results
D000473:Alkanes; D005663:Furans; D008081:Liposomes; C049732:7-O-(2,6-dideoxy-2-fluorotalopyranose)adriamycinone; D015060:1,2-Dipalmitoylphosphatidylcholine; D004317:Doxorubicin