title: Identification of extended-spectrum, AmpC, and carbapenem- hydrolyzing beta-lactamases in Escherichia coli and Klebsiella pneumoniae by disk tests.
abstract: Antibiotic disks with and without clavulanic acid, 3-aminophenylboronic acid, or EDTA were tested with a set of 55 Klebsiella pneumoniae and Escherichia coli strains producing well-characterized extended-spectrum, AmpC, or carbapenem-hydrolyzing beta-lactamases. A relatively simple scheme was devised for distinguishing beta-lactamase types in clinical isolates with or without intact outer membrane porins.
authors.affiliation: Lahey Clinic, Burlington, MA 01895, USA. george.a.jacoby@lahey.org
authors.forename: George A
authors.initials: GA
authors.lastname: Jacoby
authors.forename: Kelley E
authors.initials: KE
authors.lastname: Walsh
authors.forename: Victoria J
authors.initials: VJ
authors.lastname: Walker
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D015780:Carbapenems / Q000378:metabolism*; D002440:Cefoxitin / Q000494:pharmacology; D019818:Clavulanic Acid; D004492:Edetic Acid; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D006868:Hydrolysis; D015378:Imipenem / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology*; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods / Q000592:standards; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000145:classification* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D019818:Clavulanic Acid; D002440:Cefoxitin; D015378:Imipenem; D004492:Edetic Acid; C048795:AmpC beta-lactamases; D001618:beta-Lactamases