title: [Isolation of shiga toxin-producing Escherichia coli strains from healthy cattle of the region of Lower Silesia].
abstract: The aim of the study was to determine if cattle from the region of Lower Silesia is the reservoir of shiga toxin-producing E. coli strains (STEC) and the analysis of virulence factors of isolated STEC strains. The ability of tested animal strains to shiga toxin synthesis was analysed in cytotoxicity assay in vitro on Vero cell line and then confirmed by detection of shiga toxin-encoding genes by PCR. STEC strains were isolated from 12 (15,2%) of animals examined, 21,4% of these strains were obtained from 9 of 42 calves, and 8,1% from 3 of 37 cows. Most of STEC isolated (75%) was enterohemolysin-producing. The cattle from the region of Lower Silesia is the reservoir of pathogenic for humans sorbitol-fermenting non-O157 STEC strains.
authors.affiliation: Katedra i Zakład Mikrobiologii AM we Wrocławiu.
authors.forename: Beata
authors.initials: B
authors.lastname: Sobieszczańska
authors.forename: Romuald
authors.initials: R
authors.lastname: Gryko
authors.forename: Magdalena
authors.initials: M
authors.lastname: Dobrowolska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Błaszkowska
authors.forename: Jan
authors.initials: J
authors.lastname: Twardoń
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D004197:Disease Reservoirs / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D011044:Poland; D022621:Shiga Toxin / Q000096:biosynthesis; D013045:Species Specificity; D014774:Virulence
chemical_list: D029968:Escherichia coli Proteins; D022621:Shiga Toxin