Immunogenicity of C-terminus of Plasmodium falciparum merozoite surface protein 1 expressed as a non-glycosylated polypeptide in yeast.
The C-terminal region of the merozoite surface protein 1 (MSP119) is one of the most promising vaccine candidates against the erythrocytic forms of malaria. In the present study, a gene encoding Plasmodium falciparum MSP119 was expressed in yeast Pichia pastoris. A non-glycosylated form of the recombinant protein MSP119 was purified from culture medium. This recombinant protein maintains its antigenicity. Significant immune responses were seen in C57BL/6 mice after the second immunization. Moreover, the specific antibodies recognized the native antigens of P. falciparum. The prevailing isotypes of immunoglobulin (Ig) G associated with immunization were IgG1, IgG2a and IgG2b. The antibodies isolated from mouse sera immunized with MSP119 can inhibit parasite growth in vitro. Based on these immunological studies, we concluded that MSP119 deserves further evaluation in pre-clinical immunizations against P. falciparum.
Zhang
Zhong-Guang
ZG
Medical College of Qingdao University, Qingdao 266021, China.
Yu
Wen-Gang
WG
Qiu
Wen-Sheng
WS
Zhao
Heng-Mei
HM
D000818:Animals; D003470:Culture Media / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D006031:Glycosylation; D007074:Immunoglobulin G / Q000737:chemistry; D020066:Merozoite Surface Protein 1 / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010843:Pichia / Q000378:metabolism; D010963:Plasmodium falciparum / Q000737:chemistry* / Q000276:immunology; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry
D003470:Culture Media; D007074:Immunoglobulin G; D020066:Merozoite Surface Protein 1; D011994:Recombinant Proteins