P-CPA pretreatment reverses the changes in sleep and behavior following acute immobilization stress rats.
The effects of p-CPA (para-chlorophenylalanine) pretreatment was studied on the sleep-wake parameters and patterns of behavioral activities in an animal model of acute immobilization stress. For the experiments, young male Charles Foster rats were divided into three groups, subjected to (i) acute immobilization stress for four hours on specially designed wooden boards, (ii) a similar model of acute immobilization stress after pretreatment of p-CPA (injected through i.p. route), and (iii) control rats (p-CPA untreated and unstressed). Three channels of electrographic signals, i.e., EEG (electroencephalogram), EOG (electrooculogram), and EMG (electromyogram) were recorded continuously for four hours for all three groups of rats to analyze the changes in sleep-wake stages. The assessment of behavior was performed just after the stress on separate groups of rats in Open-Field (OF) and Elevated Plus-Maze (EPM) apparatuses. The significant changes in total sleep time (P < 0.05), total time for rapid eye movement sleep (P < 0.01), and total time in wakefulness (P < 0.01) following acute immobilization stress were found reversed in the p-CPA (a serotonin inhibitor) pretreated group of rats. Simultaneously, the results of the present work also revealed that the changes in grooming behavior (P < 0.05) in OF and the total time spent on the center of EPM (P < 0.05) were observed altered in p-CPA pretreated group of rats.
Sinha
Rakesh Kumar
RK
Department of Biomedical Instrumentation, Birla Institute of Technology, Mesra (Ranchi), Jharkhand-835215, India. rksinha_res@rediffmail.com
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D001929:Brain Edema / Q000209:etiology / Q000473:pathology; D004569:Electroencephalography; D004576:Electromyography; D004585:Electrooculography; D010134:Fenclonine / Q000494:pharmacology*; D006120:Grooming / Q000187:drug effects / Q000502:physiology; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012149:Restraint, Physical; D012702:Serotonin Antagonists / Q000494:pharmacology*; D012890:Sleep / Q000187:drug effects* / Q000502:physiology; D020178:Sleep Disorders, Circadian Rhythm / Q000503:physiopathology; D040701:Stress Disorders, Traumatic, Acute / Q000503:physiopathology*; D013997:Time Factors
D012702:Serotonin Antagonists; D010134:Fenclonine