[Effects of rapamycin on Rho-kinase and p27 mRNA expressions in a porcine coronary intimal proliferation model induced by interleukin-1beta].
OBJECTIVE
To observe the effects of rapamycin on the expressions of Rho-kinase and p27 mRNA during vascular intimal proliferation in a porcine model of coronary stenosis induced by interleukin-1beta (IL-1beta).


METHODS
The proximal segments of LAD and LCX were wrapped with cotton mesh that had absorbed sepharose bead solution with or without IL-1beta. Selective coronary angiography was performed two weeks later and the animals were killed for collecting the samples for histopathology and RT-PCR analyzing of Rho-kinase and p27 mRNA.


RESULTS
The expressions of Rho-kinase and p27 mRNA could be visualized in normal coronary wall. The expression of Rho-kinase mRNA was significantly enhanced and the expression of p27 mRNA was significantly decreased during the process of intimal proliferation induced by IL-1beta. Rapamycin significantly inhibited the intimal proliferation, reduced the infiltration of inflammatory cells, reduced the expression of Rho-kinase mRNA and increased the expression of p27 mRNA.


CONCLUSIONS
The expression of Rho-kinase mRNA is upregulated and p27 mRNA downregulated in coronary artery stenosis induced by IL-1beta and these effects could be abolished by cotreatment with rapamycin.
Miao
Zhi-lin
ZL
Deptment of Cardiovascular Disease, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zeng
Ding-yin
DY
Sun
Xi-zhuo
XZ
Zhou
Xu-chen
XC
Cheng
Ying
Y
Guan
Qi-gang
QG
Zhang
Li
L
He
Xue-zhi
XZ
Han
Feng-tong
FT
D000818:Animals; D017023:Coronary Angiography; D003331:Coronary Vessels / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D020123:Sirolimus / Q000494:pharmacology*; D013552:Swine; D017539:Tunica Intima / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D054460:rho-Associated Kinases / Q000378:metabolism*
D053583:Interleukin-1beta; D012333:RNA, Messenger; D054460:rho-Associated Kinases; D020123:Sirolimus