title: Inhibition of NTPDase, 5'-nucleotidase, Na+/K+-ATPase and acetylcholinesterase activities by subchronic treatment with Casearia sylvestris.
abstract: The aqueous extract of Casearia sylvestris was tested in cortical membrane preparations. C. sylvestris was obtained commercially from two different sources, designated as Sample A and Sample B. The enzymes studied in this work were NTPDase-like, 5'-Nucleotidase, Na(+)/K(+)-ATPase and acetylcholinesterase (AChE). Adult rats received aqueous extracts from C. sylvestris in a dose of 20mg/kg body wt. daily for a 75-day-period, by oral administration (gavage). Our study showed that this treatment caused an inhibition of NTPDase-like activity with both, ATP (19.41% with Sample A and 25.03% with Sample B) and ADP (41.57% with Sample A and 31.20% with Sample B) as substrates. This treatment also caused an inhibition of 5'-nucleotidase activity (28.34% with Sample A and 31.46% with Sample B) and Na(+)/K(+)-ATPase (25.08% with Sample A and 24.81% with Sample B). The rate of acetylcholine degradation was reduced, as shown by the inhibition of AChE (31.65% and 26.74%, Samples A and B, respectively). These results suggest that extracts of C. sylvestris can cause neurochemical alterations in the purinergic and cholinergic systems of the central nervous system.
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: da Silva
authors.forename: D
authors.initials: D
authors.lastname: Balz
authors.forename: J B D'A
authors.initials: JB
authors.lastname: de Souza
authors.forename: V M
authors.initials: VM
authors.lastname: Morsch
authors.forename: M C
authors.initials: MC
authors.lastname: Corrêa
authors.forename: G D
authors.initials: GD
authors.lastname: Zanetti
authors.forename: M P
authors.initials: MP
authors.lastname: Manfron
authors.forename: M R C
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D015720:5'-Nucleotidase / Q000037:antagonists & inhibitors; D000110:Acetylcholinesterase / Q000187:drug effects; D000818:Animals; D015703:Antigens, CD; D001081:Apyrase / Q000037:antagonists & inhibitors; D037821:Casearia / Q000737:chemistry*; D002462:Cell Membrane / Q000187:drug effects; D002540:Cerebral Cortex / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000032:analysis*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
chemical_list: D015703:Antigens, CD; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D000110:Acetylcholinesterase; D015720:5'-Nucleotidase; D001081:Apyrase; C067869:CD39 antigen; D000254:Sodium-Potassium-Exchanging ATPase