A dual superinfection and recombination within HIV-1 subtype B 12 years after primoinfection.
To analyze superinfection in an HIV-1-infected patient showing high-risk practices, viral quasispecies were analyzed in pol and env genes in several plasma samples. Phylogenetic analysis in the reverse transcriptase fragment in pol gene identified a single virus in the first 3 samples analyzed, but 12 years after primoinfection, 3 different viral strains were detected in the patient quasispecies. This result suggests a superinfection with 2 HIV-1 strains, one of which showed the T215Y + M184V resistance mutations. The analysis in the env gene confirmed the existence of 3 different strains in the viral population, one of them a recombinant. This study illustrates that events of superinfection and recombination contribute to the viral genetic variability observed in HIV-1-infected individuals.
Pernas
Maria
M
Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain.
Casado
Concepción
C
Fuentes
Rosa
R
Pérez-Elías
Maria Jesús
MJ
López-Galíndez
Cecilio
C
D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000627:therapeutic use; D018791:CD4 Lymphocyte Count; D004359:Drug Therapy, Combination; D005500:Follow-Up Studies; D015752:Genes, env / Q000235:genetics*; D015751:Genes, pol / Q000235:genetics*; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D020010:Heterosexuality; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase; D011995:Recombination, Genetic; D012309:Risk-Taking; D016415:Sequence Alignment; D013045:Species Specificity; D015819:Substance Abuse, Intravenous; D015163:Superinfection / Q000821:virology*
D019380:Anti-HIV Agents; D012194:RNA-Directed DNA Polymerase