title: Use of an on-line imprinted polymer pre-column, for the liquid chromatographic-UV absorbance determination of carbaryl and its metabolite in complex matrices.
abstract: A carbaryl and 1-naphtol molecular imprinted polymers (MIP1 and MIP 2, respectively) were prepared using suspension polymerization and tested for selective and reversible binding to carbaryl and 1-naphtol, respectively. In the suspension polymerization technique used, polymers of methacrylic acid (MAA), highly cross-linked with ethylene dimethacrylate, provide a specific binding sites for the carbaryl molecule and its metabolite when using it as a template to be removed after polymerization. The molecular imprinted polymer with a particle size of approximately 5 microm were isolated and packed into a pre-column (50 mm x 4.6 mm id) that was used to isolate carbaryl and its metabolite from complex matrices injected on a high performance liquid chromatography system using ultra-violet detection without extensive sample preparation and clean up. The HPLC method had a detection limit of 1.00 ng/mL and a linear response (r2 > 0.98) over the concentration range of 1.00-10.0 ng/mL.
authors.affiliation: Charles River Laboratories, Montreal Inc., 87 Senneville road, Senneville, Que. H9X 3R3, Canada. Jamil.Hantash@ca.crl.com
authors.forename: Jamil
authors.initials: J
authors.lastname: Hantash
authors.forename: Alan
authors.initials: A
authors.lastname: Bartlett
authors.forename: Philip
authors.initials: P
authors.lastname: Oldfield
authors.forename: Georges
authors.initials: G
authors.lastname: Dénès
authors.forename: Roger
authors.initials: R
authors.lastname: O'Rielly
authors.forename: Daniel
authors.initials: D
authors.lastname: Roudiere
authors.forename: Stephanie
authors.initials: S
authors.lastname: Menduni
mesh_terms: D000818:Animals; D012721:Carbaryl / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry; D015394:Molecular Structure; D009284:Naphthols / Q000032:analysis / Q000592:standards; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D008689:Methacrylates; D009284:Naphthols; D011108:Polymers; C008384:methacrylic acid; C029350:1-naphthol; C004919:ethylene dimethacrylate; D012721:Carbaryl