Simultaneous identification and verification of Bacillus anthracis.
Specific identification of Bacillus anthracis (B. anthracis) is vital for the accurate treatment of afflicted personnel during biological warfare situations and civilian terrorist attacks. In order to accomplish this, we have subjected the lysates from B. anthracis to affinity purification using monoclonal antibodies for the selected antigenic protein present in the bacteria. The bound antigenic protein was identified by multi-dimensional protein identification technology (MudPIT) to be a surface layer protein EA1. The same antigen was identified from the lysates from a few strains of B. anthracis demonstrating the observation to be common for B. anthracis strains. Hence, this presents an effective pathway for the identification of the bacteria present in unknown samples of various origins. Generation of a database containing the EA1 protein has been found to be useful in the database search of unknown samples.
Krishnamurthy
Thaiya
T
R&T Directorate, Edgewood Chemical and Biological Center, Aberdeen Proving Ground, MD 21010-5424, USA. thaiya.krishnamurthy@us.army.mil
Hewel
Johannes
J
Bonzagni
Neil J
NJ
Dabbs
Jason
J
Bull
Robert L
RL
Yates
John R
JR
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D001408:Bacillus anthracis / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001694:Biological Warfare; D023482:Bioterrorism; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D053719:Tandem Mass Spectrometry / Q000379:methods
D000345:Affinity Labels; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; C055764:S-layer proteins