Cytological evidence for assortment mitosis leading to loss of heterozygosity in rice.
In the root meristem cells of the rice line AMR, which causes loss of heterozygosity in its hybrids, both normal and assortment mitoses were observed. During normal mitosis, chromosomes did not form homologous pairs at metaphase; all chromosomes lined up at the equatorial plate and 2 chromatids of each chromosome disjoined at the centromere and moved toward opposite poles. During assortment mitosis, varying numbers of paired homologues were observed at mitotic metaphase. Two groups of 12 chromosomes separated and moved towards the opposite poles of daughter cells with few chromosomes having their chromatids separated at anaphase. These observations support the proposed mechanism that is responsible for early genotype fixation in rice hybrids involving AMR.
Wang
Richard R-C
RR
USDA-ARS Forage and Range Research Laboratory, Utah State University, Logan, 84322, USA. rrcwang@cc.usu.edu
Li
Xiaomei
X
Chatterton
N Jerry
NJ
D032461:Chromosomes, Plant; D019656:Loss of Heterozygosity; D008938:Mitosis / Q000235:genetics; D012275:Oryza / Q000235:genetics*