Novel furostanol saponins from the bulbs of Allium macrostemon B. and their bioactivity on [Ca2+]i increase induced by KCl.
Chemical reinvestigation of the ethanol extract of the dried bulbs of Allium macrostemon B. led to the isolation of two novel furostanol saponins, named macrostemonoside M (1) and macrostemonoside N (2), together with six known saponins. The structures of new compounds were elucidated on the basis of extensive spectroscopic analysis including 1D and 2D NMR as (25R)-22-hydroxy-5beta-furostane-1beta,2beta,3beta,6alpha-tetraol-26-O-beta-D-glucopyranoside and 22-hydroxy-5beta-furost-25-(27)-ene-1beta,2beta,3beta,6alpha-tetraol-26-O-beta-D-glucopyranoside, respectively. The pharmacological activities of all the saponins on [Ca2+]i increase induced by KCl were evaluated.
Chen
Hai-Feng
HF
Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, China.
Wang
Nai-Li
NL
Sun
Hong-Li
HL
Yang
Bao-Feng
BF
Yao
Xin-Sheng
XS
D000490:Allium / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D002478:Cells, Cultured; D006168:Guinea Pigs; D015394:Molecular Structure; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
D012503:Saponins; C511901:macrostemonoside M; C511902:macrostemonoside N; D011189:Potassium Chloride; D002118:Calcium