Isolation and characterization of a new Na+/H+ antiporter gene OsNHA1 from rice (Oryza sativa L.).
The full-length cDNA (3612 bp) of OsNHA1 was cloned by RT-PCR approach from rice (Oryza sativa L.), which encodes a putative plasma membrane Na+/H+ antiporter. Its deduced protein, OsNHA1, has 11 transmembrane domains and a significant similarity to a plasma membrane Na+/H+ antiporter AtNHA1 from Arabidopsis thaliana. Phylogenetic analysis showed that the OsNHA1 clusters with the plasma membrane Na+/H+ antiporters from various organisms. The semi-quantitative RT-PCR assay revealed that the expression of OsNHA1 was up-regulated in both shoots and roots of rice seedlings under salt stress, whereas it was not induced in the rice seedlings treated by drought stress.
Zhou
Guo-An
GA
State key laboratory of crop genetics and germplasm enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Jiang
Yan
Y
Yang
Qing
Q
Wang
Jian-Fei
JF
Huang
Ji
J
Zhang
Hong-Sheng
HS
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D002462:Cell Membrane / Q000378:metabolism; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D017421:Sequence Analysis; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics*
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; C477653:SOS1 protein, Arabidopsis; D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride