[Cloning and expression of cucumber CsEXP10 gene].
Basing on the fragment (CO434610) cloned from cDNA amplified fragment length polymorphism (cDNA-AFLP), a 1191-bp cDNA sequence of CsEXP10 with a complete 3' end was obtained via rapid amplification of cDNA ends (RACE) and getting spliced with an EST (AF319471) of the same gene (Fig.2). Southern blotting analysis showed that CsEXP10 was a single copy gene in cucumber (Fig.5). The transcripts of CsEXP10 were detected by RT-PCR only in young fruit 3 d after pollination, but not in root, stem, and leaf (Fig.4A). At different developmental stages of fruit growth, Northern blotting demonstrated that CsEXP10 gene was expressed strongly in fruit 3 d and 10 d after pollination, especially the latter, but undetectable in ovaries of 2-3 cm in length and without pollination on the day flowering, and in fruits 26 d after pollination (Fig.4B). It is suggested that CsEXP10 might be highly correlated with the expansive growth of cucumber fruit.
Sun
Yong-Dong
YD
College of Horticulture and Landscape, Southwest University, Chongqing 400716, China.
Zhang
Xing-Guo
XG
Du
Xiao-Bing
XB
Su
Cheng-Gang
CG
Bi
Xi-Hong
XH
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018553:Cucumis sativus / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005638:Fruit / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010940:Plant Proteins