Single-molecule approaches reveal the idiosyncrasies of RNA polymerases.
Recently developed single-molecule techniques have provided new insights into the function of one of the most complex and highly regulated processes in the cell--the transcription of the DNA template into RNA. This review discusses methods and results from this emerging field, and it puts them in perspective of existing biochemical and structural data.
Zlatanova
Jordanka
J
Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA. jordanka@uwyo.edu
McAllister
William T
WT
Borukhov
Sergei
S
Leuba
Sanford H
SH
D004247:DNA / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D008280:Magnetics; D018625:Microscopy, Atomic Force; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D014158:Transcription, Genetic
D004247:DNA; D012321:DNA-Directed RNA Polymerases