title: Effect of abdominal trauma on hemorrhagic shock-induced acute lung injury in rats.
abstract: OBJECTIVE
  To evaluate the effects of abdominal trauma on hemorrhagic shock-induced acute lung injury in rats.
  
  
  METHODS
  Five groups were allocated (n = 8) in the study. Group I was taken as the control group, group II as the hemorrhagic shock group, group III as hemorrhagic shock + laparotomy, group IV as hemorrhagic shock + splenectomy and group V as splenectomy + omentectomy + hemorrhagic shock group. Hemorrhagic shock was induced by drawing blood and reducing mean arterial pressure (MAP) to 40 mmHg within 10 min. After a hypotensive period of 1 h, animals were resuscitated. Bronchoalveolar lavage (BAL) was performed to recover cells from the alveolar space with 40 mL of BAL fluid after resuscitation malondialdehyde (MDA) and L-gamma-glutamyl-L-cysteinyl-glycine (GSH) levels were measured in serum, erythrocytes and lung tissue.
  
  
  RESULTS
  Serum, erythrocyte, lung tissue MDA and GSH levels were significantly increased in hemorrhagic shock groups II-V (P < 0.05). Lymphocyte, neutrophil and alveolar macrophage counts in BAL fluid indicated a significant difference between control and shock groups (P < 0.05).
  
  
  CONCLUSIONS
  The degree of trauma increases hemorrhagic shock-induced acute lung injury.
authors.affiliation: Department of Surgery, Ankara Training and Research Hospital, Ulucanlar, Ankara, Turkey. bulentkilicoglu@yahoo.com.tr
authors.forename: Bulent
authors.initials: B
authors.lastname: Kilicoglu
authors.forename: Erol
authors.initials: E
authors.lastname: Eroglu
authors.forename: Sibel-Serin
authors.initials: SS
authors.lastname: Kilicoglu
authors.forename: Kemal
authors.initials: K
authors.lastname: Kismet
authors.forename: Fusun
authors.initials: F
authors.lastname: Eroglu
mesh_terms: D000007:Abdominal Injuries / Q000150:complications* / Q000473:pathology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D004912:Erythrocytes / Q000737:chemistry / Q000473:pathology; D007813:Laparotomy; D008168:Lung / Q000737:chemistry / Q000473:pathology; D055370:Lung Injury; D008214:Lymphocytes / Q000473:pathology; D016676:Macrophages, Alveolar / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000097:blood; D009504:Neutrophils / Q000473:pathology; D009852:Omentum / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D012128:Respiratory Distress Syndrome / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D012771:Shock, Hemorrhagic / Q000150:complications* / Q000473:pathology / Q000503:physiopathology*; D013156:Splenectomy
chemical_list: D017382:Reactive Oxygen Species; D008315:Malondialdehyde