[Epstein-Barr virus (EBV) infections in patients treated with allogenic hematopoietic cells transplantation (allo-HCT)].
OBJECTIVE
Assessment of frequency and clinical course of EBV infection in patients that underwent non-manipulated allo-HCT from matched-related donors.


METHODS
Active EBV infection was confirmed based on the presence of anti-EA antibodies (ELISA) and/or viral DNA (nPCR) isolated from peripheral leukocytes. For positive DNA-isolations semi-quantitative analysis were done. Patients were examined repeatedly, the time of monitoring was approximately 6 +/- 5 months.


RESULTS
Active EBV infection was confirmed in 27 among 56 examined allo-HCT recipients. Primary infection was detected in 5 patients, in the remaining patients it was probably the result of virus reactivation. In most cases EBV-load was approximately 200 copies per 1 million of leukocytes, 1 patient with lymphoproliferative disorder (PTLD) had 2 million copies. EBV infection was asymptomatic in most cases (17), in 7 cases aminotransferase levels were insignificantly increased, in 2--diarrhea was observed and in 4 patients GvHD was intensified.


CONCLUSIONS
In recipients without risk of PTLD, permanent monitoring of the EBV-load has no clinical justification.
Zawilińska
Barbara
B
Zakład Wirusologii Katedry Mikrobiologii CM UJ w Krakowie. mbzawili@cm-uj.krakow.pl
Piatkowska-Jakubas
Beata
B
Kopeć
Jolanta
J
Daszkiewicz
Ewa
E
Kleszcz
Ewa
E
Szostek
Sława
S
Mensah-Glanowska
Patrycja
P
Hawrylecka
Dorota
D
Skotnicki
Aleksander
A
D000328:Adult; D004279:DNA, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis* / Q000821:virology*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000302:isolation & purification; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014184:Transplantation, Homologous; D019562:Viral Load
D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens