title: Reconstruction of extensive long bone defects in sheep using resorbable bioceramics based on silicon stabilized tricalcium phosphate.
abstract: In this study we evaluated the performance of Skelite, a resorbable bioceramic based on silicon stabilized tricalcium phosphate (Si-TCP), in promoting the repair of a large-sized, experimentally induced defect in a weight-bearing long bone sheep model. Eighteen 2-year-old ewes were used in this study. Animals were sacrificed at 3, 6, and 12 months. One animal entered a very prolonged followup and was sacrificed 2 years after surgery. Bone formation and scaffold resorption were evaluated by sequential x-ray studies, CT scans, histology, immunohistology, microradiography, and quantitative analysis of x-ray studies (optical density) and microradiographs (percentage of bone and scaffold area). Our data show an excellent implant integration and significant bone regeneration within the bone substitute over the course of the experiment. Progressive osteoclastic resorption of the biomaterial was also evident. At 1 year from surgery, the remaining scaffold was approximately 10-20% of the scaffold initially implanted, while after 2 years it was essentially completely resorbed. At the end of the observation period, the segmental defect was filled with newly formed, highly mineralized, lamellar bone.
authors.affiliation: Dipartimento di Oncologia, Biologia e Genetica, Università di Genova, Genova, Italy.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Mastrogiacomo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Corsi
authors.forename: Edda
authors.initials: E
authors.lastname: Francioso
authors.forename: Mariasevera
authors.initials: M
authors.lastname: Di Comite
authors.forename: Francesco
authors.initials: F
authors.lastname: Monetti
authors.forename: Silvia
authors.initials: S
authors.lastname: Scaglione
authors.forename: Angela
authors.initials: A
authors.lastname: Favia
authors.forename: Antonio
authors.initials: A
authors.lastname: Crovace
authors.forename: Paolo
authors.initials: P
authors.lastname: Bianco
authors.forename: Ranieri
authors.initials: R
authors.lastname: Cancedda
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001862:Bone Resorption; D018786:Bone Substitutes / Q000737:chemistry; D002113:Calcification, Physiologic; D002130:Calcium Phosphates / Q000737:chemistry; D002516:Ceramics / Q000737:chemistry; D005260:Female; D008422:Materials Testing; D010010:Osteoclasts / Q000166:cytology; D010012:Osteogenesis; D011859:Radiography; D012756:Sheep; D012825:Silicon / Q000737:chemistry; D013978:Tibial Fractures / Q000000981:diagnostic imaging / Q000628:therapy*
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C018392:tricalcium phosphate; D012825:Silicon