Anti-estrogen ICI 182.780 and anti-androgen flutamide induce tyrosine phosphorylation of cortactin in the ectoplasmic specialization between the Sertoli cell and spermatids in the mouse testis.
Our previous study revealed that the ectoplasmic specialization (ES) was deleted by the treatment of anti-estrogen, ICI 182.780 (ICI), and anti-androgen, flutamide (FLUT) in mouse testis. Also, expression of cortactin, an F-actin-binding protein, was decreased by the treatment of FLUT in mouse testis. Cortactin has been suggested to promote actin polymerizer at the ES in the testis, and also actin depolymerization is induced by tyrosine phosphorylation of cortactin. The present study revealed that exogenous treatment of ICI and FLUT caused the deletion of the cortactin in the apical ES and the increase of tyrosine phosphorylated cortactin in mouse testis. These results suggest that the sex hormone antagonists', ICI and FLUT, induced actin depolymerization and tyrosine phosphorylation of cortactin in the mouse testis. Also, the present study may be a key to elucidate the adverse affect exogenous compounds that affect spermiation.
Anahara
Reiko
R
Department of Bioenvironmental Medicine, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba 260-8670, Japan. re-anahara@ryotokuji-u.ac.jp
Toyama
Yoshiro
Y
Maekawa
Mamiko
M
Yoshida
Miyo
M
Kai
Masayuki
M
Ishino
Fumitoshi
F
Toshimori
Kiyotaka
K
Mori
Chisato
C
D000726:Androgen Antagonists / Q000494:pharmacology*; D000818:Animals; D051356:Cortactin / Q000378:metabolism*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology*; D005485:Flutamide / Q000494:pharmacology*; D000077267:Fulvestrant; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010766:Phosphorylation; D012708:Sertoli Cells / Q000502:physiology* / Q000648:ultrastructure; D013087:Spermatids / Q000502:physiology* / Q000648:ultrastructure; D013737:Testis / Q000187:drug effects; D014443:Tyrosine / Q000378:metabolism*
D000726:Androgen Antagonists; D051356:Cortactin; D004965:Estrogen Antagonists; D000077267:Fulvestrant; D014443:Tyrosine; D004958:Estradiol; D005485:Flutamide