[Long-term storage of obligate anaerobic microorganisms in glycerol].
We evaluated the possibility of storing the cultures of obligate anaerobic microorganisms (clostridia. acetogenic and sulfate-reducing bacteria, and methanogenic archaea) in 25% glycerol at -70 degrees C for a long time (up to 3 years). This method of storage is adequate to preserve cell viability in most obligate anaerobes.
Briukhanov
A I
AI
Netrusov
A I
AI
D001105:Archaea / Q000502:physiology; D001421:Bacteria, Anaerobic / Q000502:physiology; D003013:Clostridium / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D005990:Glycerol / Q000737:chemistry*
D003451:Cryoprotective Agents; D005990:Glycerol