title: Establishment of a novel B-cell lymphoma cell line with suppressed growth by gamma-secretase inhibitors.
abstract: A novel lymphoma cell line, designated TMD8 was established from cells of a patient with diffuse large B-cell lymphoma. TMD8 cells expressed HES1 mRNA, suggesting constitutive activation of Notch signaling. TMD8 cells expressed normal-sized Notch1 protein, and showed no mutations in the NOTCH1 gene. Cell growth was suppressed by gamma-secretase inhibitors (GSI). It was reported that GSI suppressed growth of T-cell acute lymphoblastic leukemia (T-ALL) cell lines, which frequently had NOTCH1 mutations. In addition to T-ALL, TMD8 is another unique cell line sensitive to GSI, and is useful to study effects of GSI in molecular targeting therapy.
authors.affiliation: Department of Laboratory Medicine, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-Ku, Tokyo 113-8519, Japan. tohda.mlab@tmd.ac.jp <tohda.mlab@tmd.ac.jp>
authors.forename: Shuji
authors.initials: S
authors.lastname: Tohda
authors.forename: Taku
authors.initials: T
authors.lastname: Sato
authors.forename: Hanae
authors.initials: H
authors.lastname: Kogoshi
authors.forename: Lu
authors.initials: L
authors.lastname: Fu
authors.forename: Seiji
authors.initials: S
authors.lastname: Sakano
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nara
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors*; D051792:Basic Helix-Loop-Helix Proteins / Q000187:drug effects / Q000235:genetics; D002135:Calcium-Binding Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004151:Dipeptides / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D017809:Fatal Outcome; D018398:Homeodomain Proteins / Q000187:drug effects / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D000072101:Jagged-2 Protein; D007621:Karyotyping; D008024:Ligands; D016393:Lymphoma, B-Cell / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008875:Middle Aged; D009842:Oligopeptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D051881:Receptor, Notch1 / Q000235:genetics; D051883:Receptor, Notch2 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013329:Structure-Activity Relationship; D000072056:Transcription Factor HES-1
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D053829:Amyloid Precursor Protein Secretases; D051792:Basic Helix-Loop-Helix Proteins; D002135:Calcium-Binding Proteins; D004151:Dipeptides; D004791:Enzyme Inhibitors; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; D000072101:Jagged-2 Protein; D008024:Ligands; D008565:Membrane Proteins; D009842:Oligopeptides; D012333:RNA, Messenger; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D000072056:Transcription Factor HES-1; C424126:DLL4 protein, human; C490504:JAG2 protein, human; C419410:N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester; C497761:NOTCH1 protein, human; C497764:NOTCH2 protein, human; C523463:benzyloxycarbonyl-isoleucyl-leucinal; C523462:benzyloxycarbonyl-leucyl-leucyl-norleucinal; C078493:HES1 protein, human