[HPLC-MSn analysis of trigonelline and its metabolites in rat urine].
OBJECTIVE
To establish a rapid and sensitive LC-MSn method for the identification of trigonelline and its main metabolites in rat urine.


METHODS
After optimizing the detection conditions of LC-MSn chromatography and mass spectrometry using trigonelline, its ionization and cleavage in ESI-MS and ESI-MSn modes were summarized, then serving as the basis for the metabolite analysis of trigonelline in rat urine. The 0-48 h urine samples of rats were collected after iv 8 mg x kg(-1) trigonelline, then, the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by LC-MSn.


RESULTS
The structures of trigonelline metabolites were elucidated according to the changes of the molecular weights of the metabolites (deltaM) and their cleavage pattern in ESI-ITMSn. As a result, two phase I metabolites and the parent drug were identified existing in rat urine, and two phase II metabolites were identified.


CONCLUSIONS
The LC-MSn method is rapid and high sensitive and specific, it is suitable for the identification of trigonelline and its metabolites in rat urine.
Chen
Yong
Y
Bio-Technology of Traditional Chinese Medicine Key Lab of Hubei Province, Hubei University, Wuhan, China. cy101610@npc.gov.cn
Shen
Shao-lin
SL
Chen
Huai-xia
HX
Pan
Jun
J
Han
Feng-mei
FM
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D029910:Trigonella / Q000737:chemistry
D000470:Alkaloids; D007004:Hypoglycemic Agents; C009560:trigonelline