Recovery and use of Plasmodium DNA from malaria rapid diagnostic tests.
The purpose of this study was to show that individual malaria rapid diagnosis tests (MRDTs) could also be used to isolate Plasmodium DNA for genetic studies. We extracted and amplified Plasmodium DNA using two commercial MRDT kits. Phenol/chloroform extraction followed by a nested polymerse chain reaction (PCR) can be used to identify Plasmodium falciparum and Plasmodium vivax from MRDTs. The PCR on MRDT-isolated DNA was more sensitive than antigen capture by MRDT. Satisfactory results were also obtained if older MRDT tests were used, even after long periods of storage at ambient temperature, with no special preservation.
Veron
Vincent
V
Medical Parasitology and Mycology Laboratory, Research Team EA 3593, Referral Hospital and School of Medicine, University of West Indies and French Guiana, Cayenne, French Guiana. veronvi@voila.fr
Carme
Bernard
B
D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008288:Malaria / Q000175:diagnosis / Q000469:parasitology*; D018512:Parasitemia / Q000175:diagnosis; D010961:Plasmodium / Q000235:genetics* / Q000302:isolation & purification*; D010963:Plasmodium falciparum / Q000235:genetics / Q000302:isolation & purification; D010966:Plasmodium vivax / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors
D016054:DNA, Protozoan; D011933:Reagent Kits, Diagnostic