HPLC analysis of K-48 concentration in plasma.
K-48 is a new oxime-type compound to be used as an enzyme reactivator in the treatment of exposure to organophosphorous compounds. Plasma concentration of K-48 can be determined using reversed-phase HPLC. Analysis using octyl silica stationary phase and ultraviolet-absorbance detection is fast and simple. K-48 displays a relatively high dose-normalized area under the curve as compared to pralidoxime, which might be beneficial for an antidote. After i.m. administration of 50 mumol K-48, the time course of the concentration can be approximated by a straight line between 15 and 120 min meaning the elimination follows zero-order kinetics.
Kalász
H
H
Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary. huba.kalasz@gmail.com
Hasan
M Y
MY
Sheen
R
R
Kuca
K
K
Petroianu
G
G
Ludányi
K
K
Gergely
A
A
Tekes
K
K
D000818:Animals; D000931:Antidotes; D019540:Area Under Curve; D002800:Cholinesterase Inhibitors; D002801:Cholinesterase Reactivators / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002802:Cholinesterases / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D009943:Organophosphorus Compounds / Q000009:adverse effects; D010091:Oximes / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D010599:Pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization
D000931:Antidotes; D002800:Cholinesterase Inhibitors; D002801:Cholinesterase Reactivators; C520230:K-48 compound; D009943:Organophosphorus Compounds; D010091:Oximes; D002802:Cholinesterases