G-CSF does not improve systolic function in a rat model of acute myocardial infarction.
OBJECTIVE
Granulocyte colony-stimulating factor (G-CSF) has been reported to improve cardiac performance by increasing the number of bone marrow stem cell in the peripheral circulation. The aim of this study was to investigate the impact of G-CSF administration on cardiac function in a rat model of acute myocardial infarction.


METHODS
Recombinant human G-CSF (Filgrastim, 100 microg/kg, sc) twice a day during seven consecutive days (G-CSF group, n=13) or vehicle (control group, n=10) was administrated three hours after left anterior coronary artery ligation. Cardiac performance was evaluated 19-21 days after myocardial infarction by electro- and echocardiography, hemodynamic and treadmill exercise test.


RESULTS
Both infarcted groups exhibit impaired cardiac function compared to sham-operated rats. Moreover, all cardiac functional parameters were not statistically different between G-CSF and infarcted group at resting conditions as well as after treadmill exercise stress test. There was no sign of cardiac regeneration and infarct size was not different on histological analysis between groups.


CONCLUSIONS
These data clearly shows that G-CSF treatment was unable to prevent cardiac remodeling or to improve cardiovascular function in a rat model of acute myocardial infarction, by permanent LAD ligation, despite bone marrow stem cell mobilization.
Werneck-de-Castro
João Pedro S
JP
Instituto de Biofísica Carlos Chagas Filho, UFRJ CCS, Bloco G Ilha do Fundão, 21949-900 Rio de Janeiro, RJ, Brasil.
Costa-E-Sousa
Ricardo Henrique
RH
de Oliveira
Patricia Fidelis
PF
Pinho-Ribeiro
Vanessa
V
Mello
Débora B
DB
Peçanha
Ramon
R
Mattos
Elisabete
E
Olivares
Emerson L
EL
Maia
Anna Carolina V
AC
Mill
José Geraldo
JG
Dos Santos Goldenberg
Regina Coeli
RC
Campos-de-Carvalho
Antônio Carlos
AC
D000818:Animals; D018952:Antigens, CD34 / Q000097:blood; D004195:Disease Models, Animal; D018450:Disease Progression; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D007958:Leukocyte Count; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D010805:Physical Conditioning, Animal / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013599:Systole / Q000502:physiology*; D018487:Ventricular Dysfunction, Left / Q000503:physiopathology / Q000517:prevention & control; D020257:Ventricular Remodeling / Q000502:physiology
D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor