The Cu(II)-reductase NADH dehydrogenase-2 of Escherichia coli improves the bacterial growth in extreme copper concentrations and increases the resistance to the damage caused by copper and hydroperoxide.
NADH dehydrogenase-2 (NDH-2) from Escherichia coli respiratory chain is a membrane-bound cupric-reductase encoded by ndh gene. Here, we report that the respiratory system of a ndh deficient strain suffered a faster inactivation than that of the parental strain in the presence of tert-butyl hydroperoxide due to endogenous copper. The inactivation was similar for both strains when copper concentration increased in the culture media. Furthermore, several ndh deficient mutants grew less well than the corresponding parental strains in media containing either high or low copper concentrations. A mutant strain complemented with ndh gene almost recovered the parental phenotype for growing in copper limitation or excess. Then, NDH-2 gives the bacteria advantages to diminish the susceptibility of the respiratory chain to damaging effects produced by copper and hydroperoxides and to survive in extreme copper conditions. These results suggest that NDH-2 contributes in the bacterial oxidative protection and in the copper homeostasis.
Rodríguez-Montelongo
Luisa
L
Departamento Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de Tucumán), San Miguel de Tucumán 4000, Argentina.
Volentini
Sabrina I
SI
Farías
Ricardo N
RN
Massa
Eddy M
EM
Rapisarda
Viviana A
VA
D003300:Copper / Q000378:metabolism / Q000633:toxicity*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004579:Electron Transport / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D006706:Homeostasis / Q000187:drug effects* / Q000502:physiology; D008566:Membranes / Q000201:enzymology; D009154:Mutation; D009245:NADH Dehydrogenase / Q000378:metabolism / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010088:Oxidoreductases / Q000378:metabolism / Q000502:physiology*; D010641:Phenotype; D013997:Time Factors; D020122:tert-Butylhydroperoxide / Q000378:metabolism / Q000633:toxicity*
D003470:Culture Media; D003300:Copper; D020122:tert-Butylhydroperoxide; D010088:Oxidoreductases; C122172:cupric reductase (NADH-linked); C088908:NADH dehydrogenase II; D009245:NADH Dehydrogenase