title: [Establishment of hepatic metastatic subline of nasopharyngeal carcinoma cell line].
abstract: OBJECTIVE
  To establish a hepatic metastatic subline of nasopharyngeal carcinoma (NPC) cell line.
  
  
  METHODS
  NPC cells metastatic to the liver were isolated from nude mice and the invasion and metastatic ability of the cells was observed in vivo and in vitro.
  
  
  CONCLUSIONS
  The invasion and metastasis activity of 5-8F-H3B-EGFP (an in vivo isolate with enhanced liver metastatic behaviors) were enhanced obviously in comparison with the parent cell line 5-8F-EGFP. This subline may be useful for cloning genes related to liver metastasis of NPC.
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou 510515, China. xiaochun@fimmu.com
authors.forename: Chun
authors.initials: C
authors.lastname: Han
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Kai-tai
authors.initials: KT
authors.lastname: Yao
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D004195:Disease Models, Animal; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007633:Keratins / Q000032:analysis; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation
chemical_list: D049452:Green Fluorescent Proteins; D007633:Keratins