Colocalization of interferon regulatory factor 7 (IRF7) with latent membrane protein 1 (LMP1) of Epstein-Barr virus.
Interferon regulatory factor 7 (IRF7) is one of the transcriptional factors for the activation of type I Interferon (IFN) genes. It is known that IRF7 and the latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) are highly expressed in EBV type III latency cells, and LMP1 induces mRNA expression of IRF7. In this study, the expression pattern of endogenous IRF7 was observed in several B cell lines with or without EBV infection by immunofluorescence staining. IRF7 was localized in the cytoplasm of EBV-negative B cells and EBV type I latency B cell lines. However, IRF7 was located both in the cytoplasm and nucleus of EBV type III latency cell lines. In the Jijoye cell (type III latency cell), IRF7 was colocalized with LMP1 in the cytoplasm in a capping configuration, and their interaction was confirmed by co-immunoprecipitation of LMP1 and IRF7. This colocalization was confirmed by co-transfection of IRF7 and LMP1 plasmids in EBV-negative B cells. These results suggest that the IRF7 and LMP1 interact with each other, and this may relate to the mechanism whereby LMP1 exerts functional effects in B-lymphocytes.
Kim
In-Wook
IW
Department of Applied Microbiology, College of Natural Resources, Yeungnam University, Daegu, Korea.
Park
Ho-Sun
HS
D001402:B-Lymphocytes / Q000378:metabolism / Q000821:virology; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D050839:Interferon Regulatory Factor-7 / Q000096:biosynthesis*; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D015533:Transcriptional Activation; D014763:Viral Matrix Proteins / Q000096:biosynthesis* / Q000378:metabolism
C037662:EBV-associated membrane antigen, Epstein-Barr virus; C494234:IRF7 protein, human; D050839:Interferon Regulatory Factor-7; D012333:RNA, Messenger; D014763:Viral Matrix Proteins