Genotyping of Sporothrix schenckii by analysis of ribosomal DNA regions.
An attempt was made to identify the genotypes of 31 Sporothrix schenckii strains and the relationship between the genotypes, the geographic distributions and clinical manifestations using restriction fragment length polymorphic analysis. The total DNA was extracted by cetyltrimethyl ammonium bromide. The polymorphisms were detected by hybridisation of ApaI-digested Sporothrix genomic DNA with a probe amplified from the small subunit ribosomal DNA and adjacent internal transcribed spacer regions. The band patterns manifested by Southern blotting were explored to investigate the genotypes of the 31 strains of S. schenckii collected from five different areas in China. Of the data from the 31 strains of S. schenckii studied, 15 individual patterns (DNA type A-O) were recognised. Type A-C accounted for 51.61% of all the strains. The DNA typing of S. schenckii by Southern blotting was highly sensitive and highly distinguishable. We also found a significant correlation between DNA patterns and different geographic areas and clinical manifestations.
Zhang
Zhenying
Z
Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, China.
Liu
Xiaoming
X
Yang
Guoling
G
Gao
Xiaorong
X
Jin
Liji
L
An
Lijia
L
D015139:Blotting, Southern; D004271:DNA, Fungal / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D005838:Genotype; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D013175:Sporothrix / Q000145:classification* / Q000235:genetics
D004271:DNA, Fungal; D004275:DNA, Ribosomal