title: A colorimetric assay for screening transketolase activity.
abstract: A tetrazolium red-based colorimetric assay has been devised to screen for transketolase activity with a range of aldehyde acceptors. The colorimetric TK assay is able to detect >8% bioconversion using non-alpha-hydroxylated aldehydes as acceptor substrates and is significantly faster and more convenient to use than chromatographic procedures.
authors.affiliation: Department of Chemistry, University College London, 20 Gordon Street, London WC1H OAJ, UK.
authors.forename: Mark E B
authors.initials: ME
authors.lastname: Smith
authors.forename: Ursula
authors.initials: U
authors.lastname: Kaulmann
authors.forename: John M
authors.initials: JM
authors.lastname: Ward
authors.forename: Helen C
authors.initials: HC
authors.lastname: Hailes
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D002384:Catalysis; D003124:Colorimetry / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004789:Enzyme Activation; D007659:Ketones / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D013778:Tetrazolium Salts / Q000737:chemistry; D014174:Transketolase / Q000737:chemistry*
chemical_list: D000447:Aldehydes; D007659:Ketones; D013778:Tetrazolium Salts; D014174:Transketolase