Effect of ozone produced from antibody-catalyzed water oxidation on pathogenesis of atherosclerosis.
Recent studies have suggested that antibodies can catalyze the generation of unknown oxidants including hydrogen peroxide (H2O2) and ozone (O3) from singlet oxygen (1O2) and water. This study is aimed to detect the effect of antibody-catalyzed water oxidation on atherosclerosis. Our results showed that both H2O2 and O3 were produced in human leukemia THP-1 monocytes incubated with human immunoglobulin G and phorbol myristate acetate. In the THP-1 monocytes incubated with human immunoglobulin G, phorbol myristate acetate and low density lipoprotein, the intracellular total cholesterol, free cholesterol, cholesteryl ester and lipid peroxides clearly increased, and a larger number of foam cells were observed by oil red O staining. The accumulation of all intracellular lipids was significantly inhibited by vinylbenzoic acid, and only slightly affected by catalase. These findings suggested that the production of O3, rather than H2O2, might be involved in the pathogenesis of atherosclerosis through the antibody-catalyzed water oxidation pathway.
Peng
Ke-Jun
KJ
Department of Pathophysiology, West China Preclinical Medical and Forensic Medical College, Sichuan University, Chengdu 610041, China.
Huang
Yu-Shan
YS
An
Li-Na
LN
Han
Xiao-Qun
XQ
Zhang
Jing-Ge
JG
Wang
Qiu-Lin
QL
Sun
Jing
J
Wang
Shu-Ren
SR
D050197:Atherosclerosis / Q000378:metabolism* / Q000473:pathology*; D001391:Azo Compounds / Q000494:pharmacology; D002384:Catalysis; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D007074:Immunoglobulin G / Q000737:chemistry; D008054:Lipid Peroxides / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D010126:Ozone / Q000494:pharmacology*; D013343:Styrenes / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D001391:Azo Compounds; D007074:Immunoglobulin G; D008054:Lipid Peroxides; D013343:Styrenes; D014867:Water; D010126:Ozone; D002784:Cholesterol; D006861:Hydrogen Peroxide; C011049:oil red O; D010100:Oxygen; C105064:4-vinylbenzoic acid