title: [Raising fat content in transgenic rice by anti-PEP gene transformation].
abstract: Gene for phosphoenolpyruvate carboxylase (PEPCase) was incorporated in antisense orientation into genome of japonica rice variety Xiushui 11 through Agrobacterium tumefaciens-mediated transformation of mature seeds-derived embryogenic calli, and a total of 95 transgenic plants were regenerated, which were confirmed by histochemical detection of GUS activity (Fig.2), PCR assay (Fig.3) and Southern hybridization analysis (Fig.4). The transgene was inherited by the T1 progeny and Mendelian 3:1 segregation ratio was observed in most transgenic lines. It was found that the fat content of hulled grains of T2 transgenic lines was 0.37 +/- 0.12 per cent higher than that of the control, most of which were statistically very significant (Table 2).
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Guan-Ting
authors.initials: GT
authors.lastname: Wu
authors.forename: Chun-Xiu
authors.initials: CX
authors.lastname: Lang
authors.forename: Zhang-Hua
authors.initials: ZH
authors.lastname: Hu
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Chen
authors.forename: Fu-Lin
authors.initials: FL
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Jin-Qing
authors.initials: JQ
authors.lastname: Chen
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D015139:Blotting, Southern; D016373:DNA, Antisense / Q000235:genetics*; D005223:Fats / Q000378:metabolism*; D018745:Genome, Plant / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
chemical_list: D016373:DNA, Antisense; D005223:Fats; D010730:Phosphoenolpyruvate Carboxylase