Synthesis and single enzyme activity of a clicked lipase-BSA hetero-dimer.
Click chemistry is used to construct a novel lipase-BSA hetero-dimer, in which the latter protein acts as a foot enabling the anchoring of the enzyme onto the surface for single enzyme studies.
Hatzakis
Nikos S
NS
Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands.
Engelkamp
Hans
H
Velonia
Kelly
K
Hofkens
Johan
J
Christianen
Peter C M
PC
Svendsen
Allan
A
Patkar
Shamkantr A
SA
Vind
Jesper
J
Maan
Jan C
JC
Rowan
Alan E
AE
Nolte
Roeland J M
RJ
D000818:Animals; D001203:Ascomycota / Q000201:enzymology; D002417:Cattle; D004796:Clinical Enzyme Tests; D019281:Dimerization; D004800:Enzymes, Immobilized / Q000737:chemistry; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*
D004800:Enzymes, Immobilized; D011108:Polymers; D012710:Serum Albumin, Bovine; D008049:Lipase