[Internal epidemic influenza virus proteins: isolation and investigation].
The internal influenza virus proteins M1 and RNP free from surface protein impurities were isolated from subviral particles (virions free from HA and NA ectomenes). The spikeless particles had no propensity to aggregate in the solution at pH 5.0 as compared with native viruses. The subviral particles of B/Hong Kong/330/01 influenza virus, which belonged to B/Victoria/2/87-lineage, were obtained by proteolytic treatment with the enzyme bromelain under the same conditions as in cases of influenza B viruses of B/Jamagata/16/88 lineage. A chromatographic analysis of the tryptic hydrolyzates obtained for matrix (M1) proteins of A(H1N1) and A(H3N2) influenza viruses revealed differences that were greatest between the protein M1 molecules isolated from influenza viruses of different subtypes of hemagglutinine. These findings suggest there are variations in the structure of this conservative internal viral protein M1 during evolution.
Ivanova
V T
VT
Rakutina
R O
RO
Kordiukova
L V
LV
Manykin
A A
AA
Fedorova
N V
NV
Ksenofontov
A L
AL
Slepushkin
A N
AN
D001963:Bromelains / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D004586:Electrophoresis; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry* / Q000235:genetics; D053122:Influenza A Virus, H3N2 Subtype / Q000737:chemistry* / Q000235:genetics; D016865:Reassortant Viruses / Q000737:chemistry; D012261:Ribonucleoproteins / Q000032:analysis* / Q000302:isolation & purification*; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000302:isolation & purification*; D014764:Viral Proteins / Q000302:isolation & purification*; D014771:Virion / Q000737:chemistry*
C488936:M1 protein, Influenza A virus; D012261:Ribonucleoproteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D001963:Bromelains