Extraction and detection of mRNA from horsehair.
After RNA extraction from horsehair shafts and roots, the mRNAs of beta-actin, muscle-type phosphofructokinase, and transforming growth factor-beta1 were detected by reverse transcription polymerase chain reaction assay. Low amounts of RNA were present in the horsehair. These specific mRNA transcripts were readily detected when more than three hair roots were used. However, detection of the mRNA transcripts was difficult in the hair shaft. These findings indicate that the small amounts of residual RNA in horsehair roots can be utilized as samples for molecular biological analysis.
Sato
Tetsuo
T
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
Sato
Go
G
Shoji
Youko
Y
Itou
Takuya
T
Sakai
Takeo
T
D000199:Actins / Q000032:analysis / Q000235:genetics; D000818:Animals; D006197:Hair / Q000737:chemistry*; D006736:Horses / Q000235:genetics / Q000378:metabolism; D025221:Phosphofructokinases / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000235:genetics
D000199:Actins; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D025221:Phosphofructokinases