Detection of Babesia bigemina DNA in ticks by DNA hybridization using a nonradioactive probe generated by arbitrary PCR.
The Dig-labeled probe specific to Babesia bigemina generated from monomorphic RAPD fragment of approximately 873 bp size amplified by a 10 mer CGGTGGCGAA, detected up to 100 ng of template DNA. This nonradioactive probe also detected B. bigemina in preparations of larval tick DNA from two of the five samples on dot-blot hybridization.
Ravindran
Reghu
R
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, UP, India. drreghuravi@yahoo.com
Rao
Jammi R
JR
Mishra
Ashok K
AK
D000818:Animals; D001403:Babesia / Q000145:classification / Q000302:isolation & purification*; D001404:Babesiosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000469:parasitology; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis*; D005784:Gene Amplification; D015151:Immunoblotting / Q000662:veterinary; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods / Q000662:veterinary*; D013987:Ticks / Q000469:parasitology*
D015342:DNA Probes; D016054:DNA, Protozoan