Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection.
As part of an investigation aimed at illuminating the possibilities and limits of the HOPE-fixation and paraffin-embedding technique we here describe a novel procedure which was developed in order to combine the benefits of the HOPE-technique with the capabilities of laser microdissection. The presented procedure avoids the need for amplification of template-RNA thus facilitating reliable and reproducible results. The excellent preservation of nucleic acids, proteins, and morphology in HOPE-fixed, paraffin-embedded tissues enhances the molecular applications available to date with materials acquired by laser microdissection when compared to formalin fixed, paraffin-embedded tissues, thus substantially extending the methodological panel in tissue based research.
Goldmann
Torsten
T
Clinical and Experimental Pathology, Research Center Borstel, Germany. tgoldmann@fz-borstel.de
Burgemeister
Renate
R
Sauer
Ulrich
U
Loeschke
Siegfried
S
Lang
Dagmar Silvia
DS
Branscheid
Detlev
D
Zabel
Peter
P
Vollmer
Ekkehard
E