title: Two distinct oxysterol binding protein-related proteins in the parasitic protist Cryptosporidium parvum (Apicomplexa).
abstract: Two distinct oxysterol binding protein (OSBP)-related proteins (ORPs) have been identified from the parasitic protist Cryptosporidium parvum (CpORP1 and CpORP2). The short-type CpOPR1 contains only a ligand binding (LB) domain, while the long-type CpORP2 contains Pleckstrin homology (PH) and LB domains. Lipid-protein overlay assays using recombinant proteins revealed that CpORP1 and CpORP2 could specifically bind to phosphatidic acid (PA), various phosphatidylinositol phosphates (PIPs), and sulfatide, but not to other types of lipids with simple heads. Cholesterol was not a ligand for these two proteins. CpOPR1 was found mainly on the parasitophorous vacuole membrane (PVM), suggesting that CpORP1 is probably involved in the lipid transport across this unique membrane barrier between parasites and host intestinal lumen. Although Cryptosporidium has two ORPs, other apicomplexans including Plasmodium, Toxoplasma, and Eimeria possess only a single long-type ORP, suggesting that this family of proteins may play different roles among apicomplexans.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, 4467 TAMU, College Station, TX 77483, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Zeng
authors.forename: Guran
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D016785:Cryptosporidium parvum / Q000378:metabolism*; D008024:Ligands; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D000098825:Oxysterol Binding Proteins
chemical_list: D008024:Ligands; D018129:Phosphatidylinositol Phosphates; D015800:Protozoan Proteins; D011987:Receptors, Steroid; D011994:Recombinant Proteins; D000098825:Oxysterol Binding Proteins; D002784:Cholesterol