[Osteogenic growth peptide regulates proliferation and differentiation of calvarial osteoblast-like cells of rat].
OBJECTIVE
To investigate the effect of osteogenic growth peptide(OGP) to the proliferation and differentiation of osteoblast-like cells in vitro.


METHODS
Calvarial osteoblast-like cells of rat bones were cultured individually in medium contained various concentrations (10(-11) mol/L-10(-7) mol/L) of OGP; and the cell proliferation was checked by MTT method, alkaline phosphatase activity was detected by ELISA and level of Cbfal mRNA was amplified with RT-PCR.


RESULTS
In vitro, the OGP put in culture medium resulted in an increase of cell proliferation (P < 0.05), ALP activity (P < 0.05) and level of mRNA for Cbfal (P < 0.05) respectively. All used OGP concentrations (10(-11) mol/L-10(-7) mol/L) were effective but the maximum efficiency was obtained with 10(-8) mol/L OGP.


CONCLUSIONS
Our results demonstrate that OGP stimulates proliferation and sequential differentiation in vitro of calvarial osteoblast-like cells of rat bones.
Xu
Ling
L
Department of Prosthodontics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Liang
Xing
X
Huang
Jiao
J
Dong
Qiang
Q
Xia
Lu
L
Hu
Jian
J
Zhang
Qing-hong
QH
Li
Xiao-yu
XY
D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis* / Q000235:genetics; D006657:Histones / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008297:Male; D010006:Osteoblasts / Q000166:cytology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000166:cytology*
D050659:Core Binding Factor Alpha 1 Subunit; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C074533:osteogenic growth peptide