Apyrase and 5'-nucleotidase activities in synaptosomes from the cerebral cortex of rats experimentally demyelinated with ethidium bromide and treated with interferon-beta.
Apyrase and 5'-nucleotidase activities were analyzed in an ethidium bromide (EB) demyelinating model associated with interferon-beta (IFN-beta). The animals were divided in groups: I, control (saline); II, saline and IFN-beta; III, EB and IV, EB and IFN-beta. After 7, 15 and 30 days the animals (n = 5) were sacrificed and the cerebral cortex was removed for synaptosome preparation and enzymatic assays. Apyrase activity using ATP as substrate increased in groups II, III and IV (P < 0.001) after 7 days and in groups III and IV (P < 0.001) after 15 days. Using ADP as substrate, an activation of this enzyme was observed in group III (P < 0.05) after seven and 15 days. The 5'-nucleotidase activity increased in group III (P < 0.05) after 7 days and in groups II, III and IV (P < 0.001) after 15 days. After 30 days treatment, no significant alteration was observed in enzyme activities. Results showed that apyrase and 5'-nucleotidase activities are altered in demyelination events and that IFN-beta was able to regulate the adenine nucleotide hydrolysis.
Spanevello
R M M
RM
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, RS, Brasil.
Mazzanti
C M
CM
Kaizer
R
R
Zanin
R
R
Cargnelutti
D
D
Hannel
L
L
Côrrea
M
M
Mazzanti
A
A
Festugatto
R
R
Graça
D
D
Schetinger
M R C
MR
Morsch
V M
VM
D015720:5'-Nucleotidase / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001081:Apyrase / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003711:Demyelinating Diseases / Q000139:chemically induced*; D004791:Enzyme Inhibitors / Q000633:toxicity; D004996:Ethidium / Q000633:toxicity*; D007155:Immunologic Factors / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology*; D008297:Male; D009186:Myelin Sheath / Q000187:drug effects / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000201:enzymology*
D000227:Adenine Nucleotides; D004791:Enzyme Inhibitors; D007155:Immunologic Factors; D016899:Interferon-beta; D015720:5'-Nucleotidase; D001081:Apyrase; D004996:Ethidium