[Influence of captopril on human sperm motility parameters in vitro].
OBJECTIVE
To determine the effects of captopril on human sperm motility in vitro.


METHODS
Sperm specimens were aseptically obtained by masturbation and prepared by Percoll gradient-centrifugation technique to produce a spermatozoa suspension of high motility from 12 healthy fertile men. The spermatozoa suspension were incubated with captopril at 1:9 ratio of volume at 37 degrees C in vitro, which was administrated at three different concentrations. Measurements were carried out within 5 minutes for all specimens including VAP, VSL, VCL, STR, ALH and progressive motility.


RESULTS
Within 5 minutes, three different concentrations of captopril could change the human spermatozoa motility and forward motile sperm percentage, but captopril did not decrease the other motility parameters, including VSL, VCL and VAP etc. The high concentration group could decrease the VCL more than those of the other two groups and it seemed that the velocity parameters of the middle concentration group were higher than those of the other two groups, but there were no significant difference statistically.


CONCLUSIONS
It suggests that captopril can decrease the human spermatozoa motility and forward motile sperm percentage, but it can not directly affect the spermatozoa velocity in vitro.
Yao
Hua-Xiong
HX
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan, Hubei 430030, China.
Liu
Ji-Hong
JH
D000328:Adult; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D002216:Captopril / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects
D000806:Angiotensin-Converting Enzyme Inhibitors; D002216:Captopril