Immunohistochemical analysis of subcortical white matter astroglia of infant and adult primate brains, with a note on resident neurons.
An immunohistochemical analysis of brain subcortical white matter astroglia from human (infant, adult) and adult monkey (Cebus apella, Macaca nemestrina) cases without any known neurological disease, is described. Expression of synaptic vesicle-associated proteins, excitatory amino acid transporters (EAAT1 and EAAT2) and GABAA Ralpha2 receptor produced coarse punctate labeling in human adult white matter astrocytes. A finer, generalized, punctate labeling was observed in human infants and adult C. apella monkeys. Labeling of neuronal somata and processes with microtubule-associated proteins (MAP2a-c) and neuron nuclear (NeuN) antibodies, was also observed in subcortical white matter of humans and monkeys. Results suggest competence of subcortical white matter astroglia of the primate brain to participate in various transmitter regulatory pathways. It is also proposed that, collectively with resident neurons, they may exert some role in affecting the transfer of information that takes place through the various associational and projecting fiber systems coursing through this brain compartment.
Colombo
Jorge A
JA
Unidad de Neurobiología Aplicada (UNA) (CEMIC), Av. Galván 4102, 1431 Ciudad de, Buenos Aires, Argentina. colombo@pruna.gov.ar
Bentham
Carolina
C
D000368:Aged; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D015415:Biomarkers; D001921:Brain / Q000166:cytology*; D002428:Cebus; D018031:Connexin 43; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005970:Glutamate Synthase / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008252:Macaca fascicularis; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D009478:Neuropeptide Y / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism; D011323:Primates / Q000502:physiology*; D016704:Synapsins / Q000378:metabolism; D013572:Synaptic Vesicles / Q000378:metabolism; D016708:Synaptophysin / Q000378:metabolism; D050857:Synaptotagmins / Q000378:metabolism
D015415:Biomarkers; D018031:Connexin 43; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D018377:Neurotransmitter Agents; D016704:Synapsins; D016708:Synaptophysin; D050857:Synaptotagmins; D005970:Glutamate Synthase