title: Electrochemical performance of 8-hydroxy-2'-deoxyguanosine and its detection at poly(3-methylthiophene) modified glassy carbon electrode.
abstract: 8-Hydroxy-2'-deoxyguanosine (8-OH-dG) has attracted enormous attention in recent years because it has been acknowledged as a typical biomarker of oxidative DNA damage. In this paper, the electrochemical performance of 8-OH-dG at the poly(3-methylthiophene) (P3MT) modified glassy carbon electrode (GCE) was investigated by cyclic voltammetry (CV) and linear sweep voltammetry (LSV). The conducting polymer P3MT can effectively decrease the oxidation peak potential of 8-OH-dG and greatly enhance its peak current. In 0.1 M pH 7.0 phosphate buffer solution (PBS), the anodic peak currents of cyclic voltammograms are linear with the 8-OH-dG concentration in two intervals, viz. 0.700-35.0 microM and 35.0-70.0 microM, with the correlative coefficients of 0.9992 and 0.9995, respectively. The detection limit of 8-OH-dG can be estimated to be 0.100 microM (S/N=3). This modified electrode can be used to detect the amount of 8-OH-dG in human urine. Furthermore, the effects of scan rate, pH, and interference of uric acid (UA) for the voltammetric behavior and detection of 8-OH-dG are also discussed. This proposed modified electrode also shows excellent reproducibility and stability that makes it an ideal candidate for amperometric detection of 8-OH-dG in flow injection analysis (FIA) and high performance liquid chromatography (HPLC).
authors.affiliation: Department of Chemistry, Institute of Analytical Chemistry, Liaocheng University, Liaocheng 252059, PR China.
authors.forename: Tian-Hua
authors.initials: TH
authors.lastname: Li
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Jia
authors.forename: Huai-Sheng
authors.initials: HS
authors.lastname: Wang
authors.forename: Ren-Min
authors.initials: RM
authors.lastname: Liu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002244:Carbon; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D011108:Polymers; D013876:Thiophenes
chemical_list: D011108:Polymers; D013876:Thiophenes; D002244:Carbon; C062788:poly(3-methylthiophene); D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine