Breakthrough performance of linear-DNA on ion-exchange membrane columns.
Breakthrough performance of linear-DNA adsorption on ion-exchange membrane columns was theoretically and experimentally investigated using batch and fixed-bed systems. System dispersion curves showed the absence of flow non-idealities in the experimental arrangement. Breakthrough curves were not significantly affected by flow-rate or inlet solution concentration. In the theoretical analysis a model was integrated by the serial coupling of the membrane transport model and the system dispersion model. A transport model that considers finite kinetic rate and column dispersed flow was used in the study. A simplex optimization routine coupled to the solution of the partial differential model equations was employed to estimate the maximum adsorption capacity constant, the equilibrium desorption constant and the forward interaction rate-constant, which are the parameters of the membrane transport model. Through this approach a good prediction of the adsorption phenomena is obtained for inlet concentrations and flow rates greater than 0.2 mg/ml and 0.16 ml/min.
Ma Montesinos-Cisneros
Rosa
R
Departamento de Biotecnología y Bioingeniería, CINVESTAV-IPN, Avenida IPN No. 2508, Mexico, DF 07360, Mexico. rosamamc@gauss.mat.uson.mx
Ortega
Jaime
J
Guzmán
Roberto
R
Tejeda-Mansir
Armando
A
D002852:Chromatography, Ion Exchange / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D008567:Membranes, Artificial; D008956:Models, Chemical
D008567:Membranes, Artificial; D004247:DNA