Inactivation of nanocrystalline C60 cytotoxicity by gamma-irradiation.
We investigated the effect of gamma-irradiation on the cytotoxicity of pure C60 solubilized in water by using tetrahydrofuran (THF/n-C60 or THF/n-C60). In contrast to THF/n-C60, its gamma-irradiated counterpart failed to generate oxygen radicals and cause extracellular signal-regulated kinase (ERK)-dependent necrotic cell death in various types of mammalian cells. Moreover, gamma-irradiated THF/n-C60 protected cells from the oxidative stress induced by native THF/n-C60 or hydrogen peroxide. The observed biological effects were associated with gamma-irradiation-mediated decomposition of THF and subsequent derivatization of the n-C60 surface. These results for the first time demonstrate gamma-irradiation-mediated changes in the physico-chemical properties of THF-prepared nanocrystalline C60, resulting in a complete loss of its cytotoxic effect and its conversion to a cytoprotective agent.
Isakovic
Aleksandra
A
Institute of Biochemistry, School of Medicine, 11000 Belgrade, Serbia and Montenegro.
Markovic
Zoran
Z
Nikolic
Nadezda
N
Todorovic-Markovic
Biljana
B
Vranjes-Djuric
Sanja
S
Harhaji
Ljubica
L
Raicevic
Nevena
N
Romcevic
Nebojsa
N
Vasiljevic-Radovic
Dana
D
Dramicanin
Miroslav
M
Trajkovic
Vladimir
V
D000818:Animals; D045744:Cell Line, Tumor; D037741:Fullerenes / Q000528:radiation effects / Q000633:toxicity*; D005663:Furans / Q000528:radiation effects / Q000633:toxicity; D005720:Gamma Rays; D051379:Mice; D049329:Nanostructures
D037741:Fullerenes; D005663:Furans; C018674:tetrahydrofuran; C069837:fullerene C60