Sequence analysis of the Choristoneura occidentalis granulovirus genome.
The genome of the Choristoneura occidentalis granulovirus (ChocGV) isolated from the western spruce budworm, Choristoneura occidentalis, was sequenced completely. It was 104,710 bp long, with a 67.3% A+T content and contained 116 potential open reading frames (ORFs) covering 88.4% of the genome. Of these, 29 ORFs were conserved in all fully sequenced baculovirus genomes, 30 were GV-specific, 53 were present in some nucleopolyhedroviruses (NPVs) and/or GVs, three were common to ChocGV and Choristoneura fumiferana GV (ChfuGV) and one was so far unique. To date, ChocGV is the only GV identified that contains a homologue of the apoptosis inhibitor protein P35/P49, present in some group I NPVs. It is also the first GV without a Xestia c-nigrum GV ORF 26 homologue. Five homologous regions (hrs)/repeat regions, lacking typical NPV hr palindromes were identified. ChocGV hrs were similar to each other but not to other GV hrs. A 1.8 kb repeat region with a high A+T content (81%) and multiple repeats of 21-210 bp was found between choc36 and 37. This area resembled the non-homologous region origin of DNA replication (non-hr ori) identified in Cryptophlebia leucotreta GV (CrleGV) and Cydia pomonella GV (CpGV). Based on the mean amino acid identities of homologous proteins, ChocGV was closest to fully sequenced genomes CpGV (52.3%) and CrleGV (52.1%). The closest amino acid identity was to individual ORFs from the partially sequenced ChfuGV genome (97.2% in 38 ORFs). Phylogenetic analysis placed ChocGV in a clade with CrleGV and CpGV.
Escasa
Shannon R
SR
Laboratory for Molecular Virology, Great Lakes Forestry Centre, Sault Ste Marie, ON P6A 2E5, Canada.
Lauzon
Hilary A M
HAM
Laboratory for Molecular Virology, Great Lakes Forestry Centre, Sault Ste Marie, ON P6A 2E5, Canada.
Mathur
Amanda C
AC
Laboratory for Molecular Virology, Great Lakes Forestry Centre, Sault Ste Marie, ON P6A 2E5, Canada.
Krell
Peter J
PJ
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.
Arif
Basil M
BM
Laboratory for Molecular Virology, Great Lakes Forestry Centre, Sault Ste Marie, ON P6A 2E5, Canada.
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D016367:Baculoviridae / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D025722:Granulovirus / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
D004279:DNA, Viral; D014764:Viral Proteins