JC virus chromogenic in situ hybridization in brain biopsies from patients with and without PML.
Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system caused by the JC polyoma virus. Electron microscopy and immunohistochemistry are the traditional methods of confirming the presence of the virus in brain biopsies from these patients. We studied the brain biopsies from 7 patients with PML and 6 patients without PML with chromogenic in situ hybridization (CISH) for the JC polyoma virus using a commercially available probe. The biopsies from the patients with the PML cases were proven to contain the JC polyoma virus by traditional and molecular methods. The CISH findings were compared with the known state of infection. All (7/7) of the biopsies from patients with PML were positive for the presence of polyoma virus by CISH, whereas the biopsies from patients without PML were uniformly negative. CISH seems to be a useful tool for the detection of the JC virus in brain biopsies from patients with PML, and is more accessible because a commercial probe is available.
Procop
Gary W
GW
Division of Pathology and Laboratory Medicine, The Cleveland Clinic Foundation, Cleveland, OH, USA. procopg@ccf.org
Beck
Rose C
RC
Pettay
James D
JD
Kohn
Debra J
DJ
Tuohy
Marion J
MJ
Yen-Lieberman
Belinda
B
Prayson
Richard A
RA
Tubbs
Raymond R
RR
D001921:Brain / Q000821:virology*; D016022:Case-Control Studies; D002863:Chromogenic Compounds; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D007577:JC Virus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D007968:Leukoencephalopathy, Progressive Multifocal / Q000175:diagnosis / Q000821:virology*; D016133:Polymerase Chain Reaction
D002863:Chromogenic Compounds