title: A consensus epitope prediction approach identifies the breadth of murine T(CD8+)-cell responses to vaccinia virus.
abstract: The value of predictive algorithms for identifying CD8+ T (T(CD8+))-cell epitopes has not been adequately tested experimentally. Here we demonstrate that conventional bioinformatic methods predict the vast majority of T(CD8+)-cell epitopes derived from vaccinia virus WR strain (VACV-WR) in the H-2(b) mouse model. This approach reveals the breadth of T-cell responses to vaccinia, a widely studied murine viral infection model, and may provide a tool for developing comprehensive antigenic maps of any complex pathogen.
authors.affiliation: La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill Street, Suite 326, San Diego, California 92109, USA.
authors.forename: Magdalini
authors.initials: M
authors.lastname: Moutaftsi
authors.forename: Bjoern
authors.initials: B
authors.lastname: Peters
authors.forename: Valerie
authors.initials: V
authors.lastname: Pasquetto
authors.forename: David C
authors.initials: DC
authors.lastname: Tscharke
authors.forename: John
authors.initials: J
authors.lastname: Sidney
authors.forename: Huynh-Hoa
authors.initials: HH
authors.lastname: Bui
authors.forename: Howard
authors.initials: H
authors.lastname: Grey
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sette
mesh_terms: D000465:Algorithms; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D019295:Computational Biology / Q000379:methods*; D018604:Epitope Mapping / Q000379:methods*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014616:Vaccinia virus / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte