title: A new triterpene synthase from Arabidopsis thaliana produces a tricyclic triterpene with two hydroxyl groups.
abstract: [structure: see text] Thirteen oxidosqualene cyclase homologues exist in the genome of Arabidopsis thaliana. One of these genes, At4g15340, was amplified by PCR and expressed in yeast. The yeast transformant accumulated tricyclic triterpene, (3S,13R)-malabarica-17,21-dien-3,14-diol (arabidiol), whose structure was determined by NMR and MS analyses. Its epoxide analogue, (3S,13R,21S)-malabarica-17-en-20,21-epoxy-3,14-diol (arabidiol 20,21-epoxide), was also isolated from the transformed yeast. This is the first example of a triterpene synthase that yields a tricyclic triterpene with two hydroxyl groups.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Japan.
authors.forename: Ting
authors.initials: T
authors.lastname: Xiang
authors.forename: Masaaki
authors.initials: M
authors.lastname: Shibuya
authors.forename: Yuji
authors.initials: Y
authors.lastname: Katsube
authors.forename: Takehiko
authors.initials: T
authors.lastname: Tsutsumi
authors.forename: Miyuki
authors.initials: M
authors.lastname: Otsuka
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Kazuo
authors.initials: K
authors.lastname: Masuda
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ebizuka
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D002384:Catalysis; D019751:Intramolecular Transferases / Q000378:metabolism*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D014315:Triterpenes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014315:Triterpenes; D019751:Intramolecular Transferases; C021924:lanosterol synthase