Ti-6Al-7Nb promotes cell spreading and fibronectin and osteopontin synthesis in osteoblast-like cells.
The purpose of this study was to compare the early response of human osteoblast-like cells (SaOS-2) on commercially pure titanium (cpTi) and titanium-6-aluminium-7-niobium (Ti-6Al-7Nb) using glass slide as a control. In terms of cell attachment, no significance was observed when cells were seeded on the materials. However, morphological analysis by scanning electron microscope revealed that cells on Ti-6Al-7Nb showed better spreading after 4 hrs. After 48 hrs, both Western analysis and reverse transcription polymerase chain reaction analyses showed that cells cultured on Ti-6Al-7Nb synthesized a higher amount of fibronectin and osteopontin as compared to cells seeded on cpTi or on glass slide. These results suggest that Ti-6Al-7Nb possess a good potential to support SaOS-2 cells on spreading and fibronectin and osteopontin synthesis, therefore, this material may be one of a candidate material used in implant dentistry.
Osathanon
Thanaphum
T
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Henri-Dunant Rd., Pathumwan, Bangkok, 10330, Thailand.
Bespinyowong
Kritchai
K
Arksornnukit
Mansuang
M
Takahashi
Hidekazu
H
Pavasant
Prasit
P
D000497:Alloys / Q000737:chemistry*; D000535:Aluminum / Q000737:chemistry; D018929:Cell Culture Techniques; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005353:Fibronectins / Q000096:biosynthesis*; D006801:Humans; D009556:Niobium / Q000737:chemistry; D010006:Osteoblasts / Q000502:physiology* / Q000648:ultrastructure; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000096:biosynthesis*; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
D000497:Alloys; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D009556:Niobium; D053495:Osteopontin; D000535:Aluminum; D014025:Titanium