Polysaccharide microarray technology for the detection of Burkholderia pseudomallei and Burkholderia mallei antibodies.
A polysaccharide microarray platform was prepared by immobilizing Burkholderia pseudomallei and Burkholderia mallei polysaccharides. This polysaccharide array was tested with success for detecting B. pseudomallei and B. mallei serum (human and animal) antibodies. The advantages of this microarray technology over the current serodiagnosis of the above bacterial infections were discussed.
Parthasarathy
Narayanan
N
Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA. narayanan.parthasarathy@amedd.army.mil
DeShazer
David
D
England
Marilyn
M
Waag
David M
DM
D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D042726:Burkholderia mallei / Q000276:immunology / Q000302:isolation & purification*; D016957:Burkholderia pseudomallei / Q000276:immunology / Q000302:isolation & purification*; D005896:Glanders / Q000175:diagnosis / Q000382:microbiology; D008554:Melioidosis / Q000175:diagnosis / Q000382:microbiology; D046228:Microarray Analysis / Q000379:methods*; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial