Viral nanoparticles donning a paramagnetic coat: conjugation of MRI contrast agents to the MS2 capsid.
A nanoparticle magnetic resonance imaging (MRI) contrast agent was developed by conjugation of more than 500 gadolinium chelate groups onto a viral capsid. The high density of paramagnetic centers and slow tumbling rate of modified MS2 capsids provided enhanced T1 relaxivities up to 7200 mM-1s-1 per particle. A bimodal imaging agent was generated by sequential conjugation of fluorescein and Gd3+ chelate. These results illustrate the potential for engineering natural protein assemblies to address bionanotechnology applications.
Anderson
Elizabeth A
EA
Department of Chemistry, New York University, 100 Washington Square East, New York, New York 10003, USA.
Isaacman
Steven
S
Peabody
David S
DS
Wang
Edwin Y
EY
Canary
James W
JW
Kirshenbaum
Kent
K
D001665:Binding Sites; D002213:Capsid / Q000737:chemistry* / Q000648:ultrastructure; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003287:Contrast Media / Q000737:chemistry*; D019786:Gadolinium DTPA / Q000737:chemistry*; D007089:Image Enhancement / Q000379:methods; D017909:Levivirus / Q000737:chemistry* / Q000648:ultrastructure*; D008280:Magnetics; D008422:Materials Testing; D046912:Multiprotein Complexes / Q000737:chemistry / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011485:Protein Binding
D020099:Coated Materials, Biocompatible; D003287:Contrast Media; D046912:Multiprotein Complexes; D019786:Gadolinium DTPA