Orotate phosphoribosyl transferase mRNA expression in oral squamous cell carcinoma and its relationship with the dihydropyrimidine dehydrogenase expression and the clinical effect of 5-fluorouracil.
In this study, we investigated whether orotate phosphoribosyl transferase (OPRT) correlates with the clinicopathological features and effect of 5-fluorouracil (5-FU) in human oral carcinoma. We examined the expression of OPRT mRNA by in situ hybridization in surgical specimens of oral squamous cell carcinoma. The expression of OPRT mRNA in oral carcinoma was observed in all specimens and such expression was higher than that seen in normal control tissue specimens. There was no correlation between the expression of OPRT mRNA and clinical factors, but the expression of OPRT mRNA was significantly associated with histological differentiation. The expression of OPRT mRNA showed correlation with effect of 5-FU for oral carcinoma in either in vivo or in vitro. These results suggest that the OPRT expressions may therefore be a prognostic factor of 5-FU efficacy in patients with oral squamous cell carcinoma.
Yoshitomi
Izumi
I
Division of Oral and Maxillofacial Surgery, Department of Developmental and Reconstructive Medicine, Course of Medical and Dental Science, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan.
Kawasaki
Goro
G
Yanamoto
Souichi
S
Mizuno
Akio
A
D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000032:analysis / Q000235:genetics*; D004789:Enzyme Activation; D005260:Female; D005472:Fluorouracil / Q000627:therapeutic use; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D009367:Neoplasm Staging; D009962:Orotate Phosphoribosyltransferase / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D012044:Regression Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D012333:RNA, Messenger; D042943:Dihydrouracil Dehydrogenase (NADP); D009962:Orotate Phosphoribosyltransferase; D005472:Fluorouracil