Two new bioactive triterpene glycosides from the sea cucumber Pseudocolochirus violaceus.
By activity-guided fractionation, two new triterpene glycosides, violaceusides A (1) and B (2), were isolated from the sea cucumber Pseudocolochirus violaceus as active compounds causing morphological abnormality of Pyricularia oryzae mycelia. By extensive 2D NMR techniques and chemical evidence, the structures of the two new glycosides were established as 16beta-acetoxy-3-O-[3-O-methyl-beta-D-glucopyranosyl-(1 --> 3)-beta-D-xylopyranosyl-(1 --> 4)-beta-D-quinovopyranosyl-(1 --> 2)-4-O-sodiumsulphate-beta-D-xylopyranosyl]-holosta-7,24-diene-3beta-ol (1) and 16beta-acetoxy-3-O-[3-O-methyl-beta-D-glucopyranosyl-(1 --> 3)-beta-D-xylopyranosyl-(1 --> 4)-beta-D-glucopyranosyl-(1 --> 2)-4-O-sodiumsulphate-beta-D-xylopyranosyl]-holosta-7,24-diene-3beta-ol (2), respectively. The two glycosides also exhibited significant cytotoxicity against HL-60 and BEL-7402 cancer cell lines.
Zhang
Shu-Yu
SY
School of Pharmacy, Second Military Medical University, Research Centre for Marine Drugs, Shanghai 200433, China.
Yi
Yang-Hua
YH
Tang
Hai-Feng
HF
Li
Ling
L
Sun
Peng
P
Wu
Jun
J
D000818:Animals; D045744:Cell Line, Tumor; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*; D006801:Humans; D015394:Molecular Structure; D012616:Sea Cucumbers / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*
D006027:Glycosides; D014315:Triterpenes; C511893:violaceuside A; C511892:violaceuside B