title: Nuclear ING2 expression is reduced in human cutaneous melanomas.
abstract: Cutaneous malignant melanoma is a severe and sometimes life-threatening cancer. The molecular mechanism of melanomagenesis is incompletely understood. Deregulation of apoptosis is probably one of the key factors contributing to the progression of melanoma. The inhibitor of growth (ING) family proteins are candidate tumour suppressors which play important roles in apoptosis. Downregulated expression of ING proteins have been reported in several tumour types, including the loss of nuclear expression of p33ING1b in melanoma. As ING2 exhibits 58.9% homology with p33ING1b, we hypothesized that the aberrant expression of ING2 may be involved in melanomagenesis. Here, we used tissue microarray technology and immunohistochemistry to examine ING2 expression in human nevi and melanoma biopsies. Our data showed that nuclear ING2 expression was significantly reduced in radial growth phase (RGP), vertical growth phase (VGP), and metastatic melanomas compared with dysplastic nevi (P < 0.05). Our data also revealed that nuclear ING2 expression was not associated with patient's gender, age or tumour thickness, ulceration, American Joint Committee on Cancer (AJCC) stage, tumour subtype, location and 5-year survival (P > 0.05). Taken together, our results suggest that nuclear ING2 expression is significantly reduced in human melanomas and that reduced ING2 may be an important molecular event in the initiation of melanoma development.
authors.affiliation: Department of Dermatology and Skin Science, Jack Bell Research Centre, Vancouver, BC, Canada V6H 3Z6.
authors.forename: F
authors.initials: F
authors.lastname: Lu
authors.forename: D L
authors.initials: DL
authors.lastname: Dai
authors.forename: M
authors.initials: M
authors.lastname: Martinka
authors.forename: V
authors.initials: V
authors.lastname: Ho
authors.forename: G
authors.initials: G
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002467:Cell Nucleus / Q000378:metabolism* / Q000473:pathology; D004416:Dysplastic Nevus Syndrome / Q000378:metabolism* / Q000473:pathology; D005260:Female; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D011237:Predictive Value of Tests; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology; D015996:Survival Rate; D046888:Tissue Array Analysis / Q000379:methods; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; C444654:ING2 protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D025521:Tumor Suppressor Proteins