Determination of aflatoxins in high-pigment content samples by matrix solid-phase dispersion and high-performance liquid chromatography.
A fast, efficient, and cost-effective method was developed for the analysis of aflatoxins in farm commodities with high-pigment content, such as chili powder, green bean, and black sesame. The proposed method involved matrix solid-phase dispersion (MSPD) and high-performance liquid chromatography (HPLC)-fluorescence detection (FLD) with postcolumn electrochemical derivatization in a Kobra cell. The MSPD procedure combined the extraction with neutral alumina and pigment cleanup with graphitic carbon black (GCB) in a single step. The recoveries of aflatoxins ranged from 88% to 95% with the relative standard deviations (RSD) less than 6% (n = 6). The limits of detection (LODs) were 0.25 ng/g aflatoxin B1, G1, and 0.10 ng/g aflatoxin B2, G2, respectively. The analytical results obtained by MSPD were compared to those of the immunoaffinity column (IAC) cleanup method. No significant differences were found between the two methods by t-test at the 95% confidence level.
Hu
Yan-Yun
YY
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
Zheng
Ping
P
Zhang
Zhao-Xiang
ZX
He
You-Zhao
YZ
D000348:Aflatoxins / Q000032:analysis*; D000537:Aluminum Oxide; D002212:Capsicum / Q000737:chemistry; D002244:Carbon; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000737:chemistry; D006108:Graphite; D007202:Indicators and Reagents; D010860:Pigments, Biological / Q000032:analysis*; D011786:Quality Control; D012680:Sensitivity and Specificity; D031685:Sesamum / Q000737:chemistry
D000348:Aflatoxins; D007202:Indicators and Reagents; D010860:Pigments, Biological; D002244:Carbon; D006108:Graphite; D000537:Aluminum Oxide