title: A type II-collagen derived peptide and its nitrated form as new markers of inflammation and cartilage degradation in equine osteochondral lesions.
abstract: Markers of cartilage breakdown enable studying the degradation of cartilage matrix in equine joint pathologies. This study was designed to determine the levels of Coll2-1, a peptide of the triple helix of type II collagen, and Coll2-1NO(2), its nitrated form in the plasma of healthy horses (controls; n=37) and horses suffering from osteochondrosis (n=34). Clinical and arthroscopic scores were attributed reflecting the severity of lesions and were related to the plasma levels of Coll2-1 and Coll2-1NO(2). The median of Coll2-1 was significantly higher in the control group, whereas the mean of Coll2-1NO(2) showed significant elevation in the pathological group. However, the measurement means of scoring classes did not vary significantly. The markers were able to differentiate the group of horses suffering from osteochondrosis from the group of healthy horses. The elevation of Coll2-1NO(2) in the pathological group indicates an inflammation, mediated through reactive oxygen species and/or increased myeloperoxidase activity.
authors.affiliation: Department of Clinical Sciences, Large Animal Surgery, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B41, B-4000 Liège, Belgium. m.gangl@vet-lyon.fr
authors.forename: M
authors.initials: M
authors.lastname: Gangl
authors.forename: D
authors.initials: D
authors.lastname: Serteyn
authors.forename: J-Ph
authors.initials: JP
authors.lastname: Lejeune
authors.forename: N
authors.initials: N
authors.lastname: Schneider
authors.forename: S
authors.initials: S
authors.lastname: Grulke
authors.forename: F
authors.initials: F
authors.lastname: Peters
authors.forename: T
authors.initials: T
authors.lastname: Vila
authors.forename: G
authors.initials: G
authors.lastname: Deby-Dupont
authors.forename: M
authors.initials: M
authors.lastname: Deberg
authors.forename: Y
authors.initials: Y
authors.lastname: Henrotin
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002357:Cartilage Diseases / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D024043:Collagen Type II / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006734:Horse Diseases / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D006736:Horses; D007249:Inflammation / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary; D008297:Male; D009574:Nitro Compounds / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D024043:Collagen Type II; D009574:Nitro Compounds