Regulatory variability of camalexin biosynthesis.
The anthranilate synthase ASA1, CYP79B2 and CYP71B15 (PAD3) are biosynthetic genes of the Arabidopsis phytoalexin camalexin, which are induced after pathogen infection and abiotic treatments like silver nitrate spraying. The natural variation of camalexin biosynthesis in response to Pseudomonas syringae infection was determined in several ecotypes, and differential CYP71B15 regulation as a potential basis for this variation was investigated. The expression of camalexin biosynthetic genes was restricted to the tissue undergoing cell death. After droplet infection with Alternaria alternata, a potent camalexin inducer in the Col-0 ecotype, camalexin formation and the induction of ASA1, CYP79B2 and CYP71B15 were strictly co-localized with the infection site.
Schuhegger
Regina
R
Lehrstuhl für Genetik, Technische Universität München, Am Hochanger 8, D-85350 Freising, Germany.
Rauhut
Thomas
T
Glawischnig
Erich
E
D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007211:Indoles / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D044224:Pseudomonas syringae; D012835:Silver Nitrate / Q000494:pharmacology; D013844:Thiazoles / Q000378:metabolism*; D014158:Transcription, Genetic; D000094162:Phytoalexins
D029681:Arabidopsis Proteins; D007211:Indoles; D012835:Silver Nitrate; D013844:Thiazoles; C102405:camalexin; D000094162:Phytoalexins