title: Cloning of the promoter from the gonadal aromatase gene of the European sea bass and identification of single nucleotide polymorphisms.
abstract: Aromatase contributes to sex differentiation by catalysing the conversion of androgens into estrogens. We have cloned the promoter of the gonadal aromatase gene from the sea bass, Dicentrarchus labrax, a species whose farming is complicated by male-skewed sex ratios. The promoter shows a conserved binding site for SF-1 as well as two cAMP response elements (CREs) and putative binding sites for transcription factors belonging to the Sox and forkhead families. Analysis of promoter sequences from individual fish suggests the presence of three promoter alleles that arise due to three single nucleotide polymorphisms (SNPs) in linkage disequilibrium.
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, UK.
authors.forename: Malyka
authors.initials: M
authors.lastname: Galay-Burgos
authors.forename: Claire
authors.initials: C
authors.lastname: Gealy
authors.forename: Laia
authors.initials: L
authors.lastname: Navarro-Martín
authors.forename: Francesc
authors.initials: F
authors.lastname: Piferrer
authors.forename: Silvia
authors.initials: S
authors.lastname: Zanuy
authors.forename: Glen E
authors.initials: GE
authors.lastname: Sweeney
mesh_terms: D000483:Alleles; D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001483:Base Sequence; D001492:Bass / Q000235:genetics*; D003001:Cloning, Molecular; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D006066:Gonads / Q000201:enzymology* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D054339:Steroidogenic Factor 1; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; D009841:Oligonucleotides; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D014157:Transcription Factors; D001141:Aromatase