Biochemical and biophysical studies on the precursor cells of mouse erythrocytes at different stages.
The aim of this research is to study the biochemical and biophysical properties of the precursor cells of mouse erythrocytes at different stages and the molecular mechanisms of their regulation. We investigated the degree of terminal differentiation of splenic erythroblasts obtained from mice during the acute phase of disease caused by the anemia-inducing FVAstrain of Friend virus. We analyzed the transcription and protein levels of alpha-globin, beta-globin (erythroid special protein) and GATA-1 (a special erythroid transcription factor). We also have examined the Ca2+ concentration, the distribution and amount of F-actin, important cellular components such as nucleic acids, lipids, and proteins, and the adhesion of precursor cells of RBC at different stages to vascular endothelium. Our results indicated that Ca2+ concentration and the distribution and structure of F-actin changed with the development of proerythroblasts, and that the adhesion rate between the precursor cells and endothelial cells can be correlated with the expression levels of ICAM-1 and P-selectin. These alternations caused changes in biophysical properties of the cell, such as membrane fluidity and deformability.
Wang
Jinhua
J
Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100050, USA.
Ka
Weibo
W
Sun
Dagong
D
Yao
Weijuan
W
Wen
Zongyao
Z
Chien
Shu
S
D000199:Actins / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism; D015672:Erythroid Precursor Cells / Q000378:metabolism / Q000473:pathology*; D005622:Friend murine leukemia virus; D050982:GATA1 Transcription Factor / Q000378:metabolism; D005914:Globins / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D019007:P-Selectin / Q000378:metabolism; D013154:Spleen / Q000473:pathology*; D014157:Transcription Factors
D000199:Actins; D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; D019007:P-Selectin; D014157:Transcription Factors; D018799:Intercellular Adhesion Molecule-1; D005914:Globins; D002118:Calcium