title: The effects of estradiol on estrogen receptor and glutamate transporter expression in organotypic hippocampal cultures exposed to oxygen--glucose deprivation.
abstract: The molecular basis of estrogen-mediated neuroprotection against brain ischemia remains unclear. In the present study, we investigated changes in expression of estrogen receptors (ERs) alpha and beta and excitatory amino acid transporters (EAAT) 1 and 2 in rat organotypic hippocampal slice cultures treated with estradiol and subsequently exposed to oxygen--glucose deprivation (OGD). Pretreatment with 17beta-estradiol (10 nM) for 7 days protected the CA1 area of hippocampus against OGD (60 min), reducing cellular injury by 46% compared to the vehicle control group. Levels of ERalpha protein were significantly reduced by 20% after OGD in both vehicle- and estradiol-treated cultures, whereas ERbeta was significantly up-regulated by 25% in the estradiol-treated cultures. In contrast, EAAT1 and EAAT2 levels were unchanged in response to estradiol treatment in this model of OGD. These findings suggest that estrogen-induced neuroprotection against ischemia might involve regulation of ERbeta and, consequently, of the genes influenced by this receptor.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Allegre, Brazil .
authors.forename: Helena
authors.initials: H
authors.lastname: Cimarosti
authors.forename: Ross D
authors.initials: RD
authors.lastname: O'Shea
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Jones
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Horn
authors.forename: Fabrício
authors.initials: F
authors.lastname: Simão
authors.forename: Lauren L
authors.initials: LL
authors.lastname: Zamin
authors.forename: Melissa
authors.initials: M
authors.lastname: Nassif
authors.forename: Rudimar
authors.initials: R
authors.lastname: Frozza
authors.forename: Carlos Alexandre
authors.initials: CA
authors.lastname: Netto
authors.forename: Philip M
authors.initials: PM
authors.lastname: Beart
authors.forename: Christianne
authors.initials: C
authors.lastname: Salbego
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000378:metabolism*; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism*; D027342:Excitatory Amino Acid Transporter 2 / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D000860:Hypoxia; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D027341:Excitatory Amino Acid Transporter 1; D027342:Excitatory Amino Acid Transporter 2; C493395:Slc1a2 protein, rat; C493392:Slc1a3 protein, rat; D004958:Estradiol; D005947:Glucose