title: Combining gene expression QTL mapping and phenotypic spectrum analysis to uncover gene regulatory relationships.
abstract: Gene expression QTL (eQTL) mapping can suggest candidate regulatory relationships between genes. Recent advances in mammalian phenotype annotation such as mammalian phenotype ontology (MPO) enable systematic analysis of the phenotypic spectrum subserved by many genes. In this study we combined eQTL mapping and phenotypic spectrum analysis to predict gene regulatory relationships. Five pairs of genes with similar phenotypic effects and potential regulatory relationships suggested by eQTL mapping were identified. Lines of evidence supporting some of the predicted regulatory relationships were obtained from biological literature. A particularly notable example is that promoter sequence analysis and real-time PCR assays support the predicted regulation of protein kinase C epsilon (Prkce) by cAMP responsive element binding protein 1 (Creb1). Our results show that the combination of gene eQTL mapping and phenotypic spectrum analysis may provide a valuable approach to uncovering gene regulatory relations underlying mammalian phenotypes.
authors.affiliation: Department of Molecular Sciences, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, TN 38163, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.forename: Lai
authors.initials: L
authors.lastname: Wei
authors.forename: Jeremy L
authors.initials: JL
authors.lastname: Peirce
authors.forename: Ramin
authors.initials: R
authors.lastname: Homayouni
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Li
authors.forename: Mi
authors.initials: M
authors.lastname: Zhou
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Robert W
authors.initials: RW
authors.lastname: Williams
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: Pfeffer
authors.forename: Dan
authors.initials: D
authors.lastname: Goldowitz
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D041681:NIH 3T3 Cells; D016716:PC12 Cells; D010641:Phenotype; D040641:Quantitative Trait Loci; D051381:Rats