[Effects of nonylphenol on testis tissue development and apoptosis of F1 generation male SD rats in weaning].
OBJECTIVE
To study the mechanism of nonylphenol on testis tissue development and apoptosis of F1 generation male SD rats in weaning period.


METHODS
Nonylphenol was administrated via gastropipe to pregnant rats at doses of 50, 100 and 200 mg/kg, respectively, from the 7th day of preganacy to weaning. F1 generation male SD rats were sacrificed in the weaning period; their serum levels of nonylpheol were determined. The histopathological examination and immunohistochemical analysis(Bax,Bcl-2,Caspase-3) on testis were also carried out.


RESULTS
Compared with control, a higher serum nonylpheol level was found in F1 generation rats treated with nonylpheol 100 and 200 mg/kg. Histopathological examination revealed that rats treated with 200 mg/kg nonylphenol had much smaller diameter of testis seminiferous tubules when compared with those in the control group. Immunohistological analysis showed that the expression of Caspase-3 and Bax in the testis seminiferous tubules increased while the expression of Bcl-2 decreased in three experiment groups.


CONCLUSIONS
All the above data proved that nonylphenol, after being given to pregnant rats, caused apparent damage to the development of testis of F1 generation male SD rats in weaning period.
Zhang
Hao
H
Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Long
Dong-mei
DM
Zhan
Li
L
Wu
De-sheng
DS
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000096:biosynthesis; D005260:Female; D008297:Male; D009929:Organ Size; D010636:Phenols / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000166:cytology / Q000254:growth & development*; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
D010636:Phenols; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C025256:nonylphenol; D053148:Caspase 3