Bombyx mori cell line as a model of immune-system organs.
We tested 11 Bombyx mori cell lines for induction of cecropin B gene (CecB) expression. After the immune challenge, CecB expression was induced in seven cell lines. A mixture of the cell-free supernatant from the immune-responsive cell lines and lipopolysaccharide activated a promoter of CecB in the non-immune-responsive cell line, indicating that secreted factor(s) is involved in CecB activation. The expressed sequence tags of one of the immune-responsive cell lines, NISES-BoMo-Cam1, contained genes encoding proteins similar to Relish, Cactus, clip-domain serine protease, serpin, lectin, peptidoglycan recognition protein, 6tox and gloverin, in addition to seven known B. mori immune-inducible genes. These results show that NISES-BoMo-Cam1 cells can be used as an in vitro model of the immune system organs of B. mori.
Taniai
K
K
Insect Biotechnology and Sericology Department, National Institute of Agrobiological Sciences, Ibaraki, Japan. taniai@affrc.go.jp
Lee
J H
JH
Lee
I H
IH
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D001483:Base Sequence; D012831:Bombyx / Q000166:cytology / Q000276:immunology*; D002460:Cell Line; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation / Q000276:immunology*; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008954:Models, Biological; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000737:chemistry / Q000235:genetics
D000900:Anti-Bacterial Agents; D019476:Insect Proteins; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; C107617:gloverin; C031162:cecropin B protein, Insecta