From genetical genomics to systems genetics: potential applications in quantitative genomics and animal breeding.
This article reviews methods of integration of transcriptomics (and equally proteomics and metabolomics), genetics, and genomics in the form of systems genetics into existing genome analyses and their potential use in animal breeding and quantitative genomic modeling of complex traits. Genetical genomics or the expression quantitative trait loci (eQTL) mapping method and key findings in this research are reviewed. Various procedures and potential uses of eQTL mapping, global linkage clustering, and systems genetics are illustrated using actual analysis on recombinant inbred lines of mice with data on gene expression (for diabetes- and obesity-related genes), pathway, and single nucleotide polymorphism (SNP) linkage maps. Experimental and bioinformatics difficulties and possible solutions are discussed. The main uses of this systems genetics approach in quantitative genomics were shown to be in refinement of the identified QTL, candidate gene and SNP discovery, understanding gene-environment and gene-gene interactions, detection of candidate regulator genes/eQTL, discriminating multiple QTL/eQTL, and detection of pleiotropic QTL/eQTL, in addition to its use in reconstructing regulatory networks. The potential uses in animal breeding are direct selection on heritable gene expression measures, termed "expression assisted selection," and genetical genomic selection of both QTL and eQTL based on breeding values of the respective genes, termed "expression-assisted evaluation."
Kadarmideen
Haja N
HN
Statistical Animal Genetics Group, Institute of Animal Science, Swiss Federal Institute of Technology, ETH Zentrum (UNS D7), Universitaetstrasse 65, CH 8092 Zürich, Switzerland. haja.kadarmideen@inw.agrl.ethz.ch
von Rohr
Peter
P
Janss
Luc L G
LL
D000818:Animals; D001947:Breeding / Q000379:methods; D019295:Computational Biology / Q000379:methods; D023281:Genomics / Q000379:methods; D049490:Systems Biology / Q000379:methods