title: Choroid plexus tumors differ from metastatic carcinomas by expression of the excitatory amino acid transporter-1.
abstract: Tumors of the choroid plexus (CPTs) are rare neoplasms of neuroectodermal origin usually arising in pediatric patients. However, CPT may occur at any age, and their distinction from metastatic carcinomas is often difficult in adult cases. Because CPTs frequently show focal glial differentiation, we now investigated 35 CPTs (19 males and 16 females 0.3-70 years old; median age, 25.0 years), including 21 choroid plexus papillomas (CPPs), 5 atypical CPP, and 9 choroid plexus carcinomas regarding their expression of the excitatory amino acid transporter-1 (EAAT1, corresponding to rodent GLAST/GLAST-1) by immunohistochemistry. In addition, 77 metastatic carcinomas, including 64 adenocarcinomas with mostly papillary formations, derived from different organs were examined. Of the 35 CPTs, 23 (66%) showed membranous EAAT1 expression in variable numbers of tumor cells, including all atypical CPP and 3 of 9 choroid plexus carcinomas (33%). None of the metastatic carcinomas showed membranous immunostaining. Excitatory amino acid transporter-1 expression in CPT was significantly age dependent (P < .0001), with the proportion of EAAT1-positive tumor cells increasing with age, but not sex dependent. There was a highly significant difference between EAAT1 expression in CPT and in metastatic carcinomas (P < .0001). Establishing a cutoff value of 1% immunoreactive tumor cells served in adult cases to distinguish CPT from metastatic adenocarcinomas with 100% specificity and 70% sensitivity and was associated with positive and negative predictive values of 100% and 91%, respectively. Our findings indicate that EAAT1 immunohistochemistry may be useful in differentiating CPT from metastatic carcinomas.
authors.affiliation: Institute of Brain Research (Neuropathology), Eberhard-Karls-University, Tuebingen, Germany. rudi.beschorner@med.uni-tuebingen.de
authors.forename: Rudi
authors.initials: R
authors.lastname: Beschorner
authors.forename: Jens
authors.initials: J
authors.lastname: Schittenhelm
authors.forename: Heiko
authors.initials: H
authors.lastname: Schimmel
authors.forename: José R
authors.initials: JR
authors.lastname: Iglesias-Rozas
authors.forename: Tina
authors.initials: T
authors.lastname: Herberts
authors.forename: Holger
authors.initials: H
authors.lastname: Schlaszus
authors.forename: Richard
authors.initials: R
authors.lastname: Meyermann
authors.forename: Manfred
authors.initials: M
authors.lastname: Wehrmann
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D016545:Choroid Plexus Neoplasms / Q000378:metabolism* / Q000473:pathology; D003937:Diagnosis, Differential; D027341:Excitatory Amino Acid Transporter 1 / Q000096:biosynthesis*; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D017599:Neuroectodermal Tumors / Q000378:metabolism*; D012737:Sex Factors
chemical_list: D027341:Excitatory Amino Acid Transporter 1