[Mutation of mitochondrial DNA in patients with non-alcoholic steatohepatitis].
When the numbers of mutations per 1,000 bases of mitochondrial DNA were compared among three groups of Al-Hep, NASH and FL, there were significantly more mutations in ND1 and COII of Al-Hep and NASH than in FL. When details of the base mutation were investigated by group, the transition type of mutation between T:A and C:G occurred in control and coding regions. In FLS and FLS-ob mice, mutations in the D-loop and coding regions of mitochondrial DNA were investigated by sequencing analysis. The average number of mutations per clone in each region was more than 5-fold higher in FLS-ob than in FLS. In addition, the mutations were mostly transition-type mutations. These data indicate that nitric oxide plays an important role in mitochondrial DNA mutations.
Kawahara
Hiromu
H
Department of Gastroenterology, Kanazawa Medical University.
Takase
Shujiro
S
D000818:Animals; D050260:Carbohydrate Metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D005234:Fatty Liver / Q000235:genetics*; D006801:Humans; D007328:Insulin / Q000502:physiology; D050356:Lipid Metabolism; D051379:Mice; D009154:Mutation; D009569:Nitric Oxide / Q000502:physiology; D009765:Obesity / Q000378:metabolism
D004272:DNA, Mitochondrial; D007328:Insulin; D009569:Nitric Oxide