[Quality standard for Xindi soft capsule].
OBJECTIVE
To establish the quality control standard of Xindi soft capsule.


METHODS
Quercetin, kaempferol and isorhamnetin were isolated by TLC with chloroform-ethyl formate-formic acid (5:4:1). The chromatographic separation was performed on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm). Acetonitrile-water-phosphoric (30:70:0.1) as mobile phase. The flow rate was 1 mL x min(-1) and column temperature was set at 40 degrees C. The UV detection wavelength was set at 254 nm.


RESULTS
Quercetin, Kaempferol and Isorhamnetin could be identified by TLC. Quercetin showed a good linear relationship at a range of 0.412-1.648 microg, r = 0.999 9, the average recovery was 96.8%, and RSD was 0.9% (n = 6). Kaempferol showed a good linear relationship at a range of 0.021-0.083 microg, r = 0.999 8, the average recovery was 96.9%, and RSD was 2.0% (n = 6). Isorhamnetin showed a good linear relationship at a range of 0.183-0.732 microg, r = 0.999 9, the average recovery was 97.1%, and RSD was 1.6% (n = 6).


CONCLUSIONS
The method is accurate with the good reproducibility and can be used for the quality control of Xindi soft capsule.
Wang
Yu-Jie
YJ
College of Traditional Chinese Pharmacy, Chengdu University of Traditional Chinese medicine, Chengdu 611731, China. superwangyj@163.com
Zhang
Yi
Y
Gu
Rui
R
Lai
Xian-Rong
XR
Wang
Ping
P
Zhang
Zhu-Jun
ZJ
D002214:Capsules; D002855:Chromatography, Thin Layer; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D044948:Flavonols / Q000032:analysis; D031281:Hippophae / Q000737:chemistry*; D044949:Kaempferols / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D011794:Quercetin / Q000032:analysis / Q000031:analogs & derivatives; D015203:Reproducibility of Results
D002214:Capsules; D004365:Drugs, Chinese Herbal; D044948:Flavonols; D044949:Kaempferols; C047368:3-methylquercetin; C006552:kaempferol; D011794:Quercetin