title: [Study on monoamine neurotransmitters in rat serum and brain tissue by high performance liquid chromatography with electrochemical detection].
abstract: OBJECTIVE
  A rapid, reliable and simple method detecting monoamine neurotransmitters in rat serum and brain tissue by high performance liquid chromatography with electrochemical detector was developed.
  
  
  METHODS
  An ODS column was selected as separation column at room temperature,and the mobile phase (pH4.50) consisted of 0.02 mol/L trisodium citrate and 0.05 mol/L disodium phosphate, containing 15% methanol (volume ratio)in distilled water. The mobile phase was pumped at a flow rate of 1.0 ml/min and the oxidation potential was set at +0.065 mV. Dihydroxybenzylamine was used as the internal standard for quantification. Four compounds, epinephrine (E), dopamine (DA) and 5-hydroxytryptamine(5-HT) in the brain tissue ((whole cerebral cortex, hippocampus, striatum, midbrain, cerebellum) and serum were simultaneously separated and determined under the above conditions.
  
  
  RESULTS
  RSD of the retention times and peak areas of standard sample was in the range 0.29%-0.67% and 0.09%-0.36%. The recoveries of all analytes were over 85%. The detection limits of 0.083 ng/ml for NE, 0.51 ng/ml for E, 0.046 ng/ml for DA and 0.078ng/ml for 5-HT were achieved with standard solutions. The linearity is over the range 1.0-100 ng/ml for NE, 5.0-80 ng/ml for E,1.0-80 ng/ ml for DA and 1.0-60 ng/ml for 5-HT.
  
  
  CONCLUSIONS
  The assay had been applied successfully to measure simultaneously cortex and serum concentrations of these four monoamines in rats.
authors.affiliation: School of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Guo-hong
authors.initials: GH
authors.lastname: Ruan
authors.forename: Li-xing
authors.initials: LX
authors.lastname: Zheng
authors.forename: Qiang-en
authors.initials: QE
authors.lastname: Wu
authors.forename: Zhi-jun
authors.initials: ZJ
authors.lastname: Zhou
mesh_terms: D000818:Animals; D015306:Biogenic Monoamines / Q000097:blood* / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000097:blood* / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods; D004837:Epinephrine / Q000097:blood* / Q000378:metabolism; D008297:Male; D018377:Neurotransmitter Agents / Q000097:blood* / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000097:blood / Q000378:metabolism
chemical_list: D015306:Biogenic Monoamines; D018377:Neurotransmitter Agents; D012701:Serotonin; D004298:Dopamine; D004837:Epinephrine