title: Comparison of vitellogenin induction, sex ratio, and gonad morphology between zebrafish and Japanese medaka after exposure to 17alpha-ethinylestradiol and 17beta-trenbolone.
abstract: The pharmaceutical estrogen 17alpha-ethinylestradiol (EE2) and the anabolic androgen 17beta-trenbolone (Tb) can interfere with the endocrine and reproductive systems of fish. The potency of these chemicals in zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) was assessed using the core end points vitellogenin (Vtg) concentration at 38 days post-hatch and sex ratio and gonad morphology at 60 days post-hatch. Vtg concentrations were measured in fish whole-body homogenate samples using enzyme-linked immunosorbent assay. Increased Vtg concentration and feminization of fish after exposure to 10 ng/L EE2, as well as masculinization after exposure to 50 ng/L of Tb, were observed in zebrafish. Intersex was observed in medaka exposed to EE2. A decrease in Vtg production after Tb exposure (50 ng/L) was measured in both zebrafish and medaka. Analyses of gonad morphology revealed increased testicular area and sperm percentage in Tb-exposed zebrafish, whereas increased sperm percentage was measured in Tb-exposed medaka. The higher sensitivity of zebrafish compared with medaka to both EE2 and Tb was revealed in the study.
authors.affiliation: Department of Biomedical Sciences and Veterinary Public Health, Division of Pathology, Pharmacology, and Toxicology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Box 7028, SE-750 07, Uppsala, Sweden. stefan.orn@bvf.slu.se
authors.forename: S
authors.initials: S
authors.lastname: Orn
authors.forename: S
authors.initials: S
authors.lastname: Yamani
authors.forename: L
authors.initials: L
authors.lastname: Norrgren
mesh_terms: D000818:Animals; D004997:Ethinyl Estradiol / Q000633:toxicity*; D005260:Female; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development; D009990:Oryzias / Q000502:physiology*; D012744:Sex Ratio; D013094:Spermatozoa / Q000187:drug effects / Q000254:growth & development; D013737:Testis / Q000187:drug effects / Q000254:growth & development; D014204:Trenbolone Acetate / Q000633:toxicity*; D014819:Vitellogenins / Q000032:analysis; D015027:Zebrafish / Q000502:physiology*
chemical_list: D014819:Vitellogenins; D004997:Ethinyl Estradiol; D014204:Trenbolone Acetate