Hydrothermal treatment of Novelose 330 results in high yield of resistant starch type 3 with beneficial prebiotic properties and decreased secondary bile acid formation in rats.
Annealing and heat-moisture treatment (HMT) are shown to be suitable methods to increase the yield of resistant starch type 3 (RS3) from Novelose 330 by up to 75%. Peak temperatures of approximately 121 degrees C were used to produce to a sufficiently high thermal stability of the hydrothermal modified RS3 products for a wide range of applications. HMT significantly increased the crystallinity up to 40%. An in vivo feeding experiment with Wistar rats showed that fermentation of Novelose 330 dominated in the proximal colon, but degradation of HMT-Novelose was more dominant in the distal colon, leading to higher butyrate concentrations in this segment of the large bowel. Large-bowel surface and crypt length increased in the proximal colon in rats fed the Novelose 330-containing diet. In contrast, after the intake of HMT-Novelose, maximal values were found in the distal segment. The lower pH and higher butyrate concentration of the caecal and colonic contents significantly suppressed the formation of secondary bile acids in RS3-fed rats. The formation of secondary bile acids was inhibited more strongly by HMT-Novelose than by Novelose 330. The Ki-67-immunopositive epithelial cells in the colon of RS3-fed rats indicated the establishment of an optimal balance in the dynamic process of mucosal regeneration. HMT provides a method for the economical production of a high-quality RS3 with dominating prebiotic properties in the distal colon for health-promoting applications.
Jacobasch
Gisela
G
German Institute of Human Nutrition Potsdam-Rehbruecke, Research Group Food Chemistry and Preventive Nutrition, D-14558 Nuthetal, Germany. G.K.Jacobasch@t-online.de
Dongowski
Gerhard
G
Schmiedl
Detlef
D
Müller-Schmehl
Katrin
K
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D002151:Calorimetry; D049109:Cell Proliferation; D003106:Colon / Q000166:cytology / Q000378:metabolism; D004063:Digestion / Q000502:physiology*; D005232:Fatty Acids, Volatile / Q000032:analysis / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D005766:Gastrointestinal Contents / Q000737:chemistry; D006358:Hot Temperature; D008297:Male; D019936:Probiotics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013213:Starch; D014961:X-Ray Diffraction
D001647:Bile Acids and Salts; D005232:Fatty Acids, Volatile; D013213:Starch