Optic nerve alterations in PTEN +/- mice.
OBJECTIVE
To study the morphologic characteristics of the optic nerve (ON) by using an experimental model of knockout mice for the expression of the PTEN gene, mainly involved in cell cycle arrest, apoptosis control, and cell size regulation.


METHODS
The eyeballs with the retrobulbar ON attached were obtained from 26-week-old mice. By using morphologic and morphometric techniques, light and electron transmission microscopy, the ON characteristics were determined in two groups of mice: 1) 'wild type' mice as the control group (C-G; n=15), 2) heterozygous knockout mice (+/-) for the PTEN gene (PTEN-G; n=15). Glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) were studied using Western blot and immunoblotting approaches.


RESULTS
The ON cross-sectional area was significantly higher in the PTEN-G than in the C-G (p<0.001). The axon sizes in mutant animals were much larger than in wild-type mice (p<0.001). No significant differences were noticed between those groups regarding the number of axons forming the ON and the presence of intra-axonal degeneration, myelin sheath alterations, or axoplasm density. No differences were detected in developmental marker protein expression.


CONCLUSIONS
The morphologic and morphometric results suggest that heterozygous PTEN knockout mice had hypertrophic ON without ultrastructural alterations.
Lopez-Sanchez
E
E
Hospital Arnau de Vilanova, Valencia--Spain. elopsan@ono.com
Frances-Muñoz
E
E
Menezo
J L
JL
Pinazo-Duran
M D
MD
D000818:Animals; D001369:Axons; D015153:Blotting, Western; D048429:Cell Size; D005786:Gene Expression Regulation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006984:Hypertrophy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D004676:Myelin Basic Protein / Q000378:metabolism; D009900:Optic Nerve / Q000378:metabolism / Q000473:pathology*; D051059:PTEN Phosphohydrolase / Q000235:genetics*
D005904:Glial Fibrillary Acidic Protein; D004676:Myelin Basic Protein; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse