Adipose tissue production of hepatocyte growth factor contributes to elevated serum HGF in obesity.
Serum HGF is elevated in obese individuals. This study examined the contribution of excess adipose tissue to increased circulating HGF levels in obesity. Serum HGF was measured by ELISA before and after weight loss due to bariatric surgery or a 24-h fast. At 6.1 +/- 0.1 mo following surgery, BMI (50.6 +/- 1.6 vs. 35.1 +/- 1.3 kg/m(2); P < 0.0001) and serum HGF were significantly decreased (1,164 +/- 116 vs. 529 +/- 39 pg/ml, P < 0.001). A 24-h fast did not change serum HGF, but serum leptin was significantly reduced (67.7 +/- 7.1 vs. 50.3 +/- 8.3 ng/ml, P = 0.02). HGF secretion in vitro from adipocytes of obese (BMI 40.3 +/- 2.8 kg/m(2)) subjects was significantly greater (80.9 +/- 10.4 vs. 21.5 +/- 4.0 pg/10(5) cells, P = 0.008) than release from adipocytes of lean (BMI 23.3 +/- 1.4 kg/m(2)) subjects. HGF mRNA levels determined by real-time RT-PCR were not different in adipocytes from lean (BMI 24.0 +/- 0.8 kg/m(2)) and obese (45.7 +/- 3.0 kg/m(2)) subjects, but serum HGF was significantly elevated in the obese individuals studied (787 +/- 61 vs. 489 +/- 49 pg/ml, P = 0.001). TNF-alpha (24 h treatment) significantly increased HGF release from subcutaneous adipocytes 23.6 +/- 8.3% over control (P = 0.02). These data suggest that elevated serum HGF in obesity is in part attributable to excess adipose tissue and that this effect can be reversed by reducing adipose tissue mass through weight loss. Increased HGF secretion from adipocytes of obese subjects may be due to posttranscriptional events possibly related to adipocyte size and stimulation by elevated TNF-alpha in the adipose tissue of obese individuals.
Bell
Lauren N
LN
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 541 North Clinical Drive, Rm. CL455, Indianapolis, IN 46202-5111, USA.
Ward
Jennifer L
JL
Degawa-Yamauchi
Mikako
M
Bovenkerk
Jason E
JE
Jones
RoseMarie
R
Cacucci
Brenda M
BM
Gupta
Christine E
CE
Sheridan
Carol
C
Sheridan
Kevin
K
Shankar
Sudha S
SS
Steinberg
Helmut O
HO
March
Keith L
KL
Considine
Robert V
RV
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D050110:Bariatric Surgery; D001706:Biopsy; D005260:Female; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis* / Q000097:blood* / Q000235:genetics; D006801:Humans; D020738:Leptin / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000378:metabolism* / Q000601:surgery; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015431:Weight Loss / Q000502:physiology*
D020738:Leptin; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D017228:Hepatocyte Growth Factor