Protein biomarkers in a mouse model of extremes in trait anxiety.
Brain proteome analysis of mice selectively bred for either high or low anxiety-related behavior revealed quantitative and qualitative protein expression differences. The enzyme glyoxalase-I was consistently expressed to a higher extent in low anxiety as compared with high anxiety mice in several brain areas. The same phenotype-dependent difference was also found in red blood cells with normal and cross-mated animals showing intermediate expression profiles of glyoxalase-I. Another protein that showed a different mobility during two-dimensional gel electrophoresis was identified as enolase phosphatase. The presence of both protein markers in red or white blood cells, respectively, creates the opportunity to screen for their expression in clinical blood specimens from patients suffering from anxiety.
Ditzen
Claudia
C
Max Planck Institute of Psychiatry, D-80804 Munich, Germany.
Jastorff
Archana M
AM
Kessler
Melanie S
MS
Bunck
Mirjam
M
Teplytska
Larysa
L
Erhardt
Angelika
A
Krömer
Simone A
SA
Varadarajulu
Jeeva
J
Targosz
Bianca-Sabrina
BS
Sayan-Ayata
Eser F
EF
Holsboer
Florian
F
Landgraf
Rainer
R
Turck
Christoph W
CW
D000679:Amygdala / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001007:Anxiety / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism*; D001522:Behavior, Animal; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D015153:Blotting, Western; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000378:metabolism; D008297:Male; D051379:Mice; D010751:Phosphopyruvate Hydratase / Q000378:metabolism
D015415:Biomarkers; D001798:Blood Proteins; D010751:Phosphopyruvate Hydratase; D007791:Lactoylglutathione Lyase