Proteomic analysis of rice leaf, stem and root tissues during growth course.
Rice proteins were isolated from leaf, stem and root tissues, harvesting at 1, 2, 4, 8 and 10 weeks after budding. Each tissue of each age was separately pulverized in liquid nitrogen, and the resulted tissue powders were suspended in 10% TCA-acetone and followed by acetone suspension to precipitate at low temperature, which resulted in the tissue-specific and age-specific protein mixture. The protein mixtures were separated by 2-DE using polyacrylamide gels (26 x 20 cm). The protein spots were identified by N-terminal sequence analysis and by MALDI and LC-MS/MS analyses after in-gel tryptic digestion. From a total of 4532 spots, 676 unique proteins were identified, of which 80 proteins (12%) were observed in all three tissues: leaf, stem and root. In addition, 45 (7%) were common in leaf and stem, 57 (8%) in stem and root, and 10 (2%) proteins in root and leaf. Also 141 unique proteins (21%) were observed only for leaf, 96 (14%) for stem, and 247 (36%) for root tissue. Proteins playing a role for photosynthesis and energy production were most abundant in leaf and stem, and those for cell defense were rich in roots.
Nozu
Yuzo
Y
Proteomics Research Laboratory, Tokyo Rikakikai Co., Tsukuba, Japan.
Tsugita
Akira
A
Kamijo
Kenichi
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D012275:Oryza / Q000737:chemistry* / Q000254:growth & development*; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D000596:Amino Acids; D010446:Peptide Fragments; D010940:Plant Proteins; D020543:Proteome; D014357:Trypsin