Use of superparamagnetic beads for the isolation of a peptide with specificity to cymbidium mosaic virus.
A modified method for the rapid isolation of specific ligands to whole virus particles is described. Biopanning against cymbidium mosaic virus was carried out with a commercial 12-mer random peptide display library. A solution phase panning method was devised using streptavidin-coated superparamagnetic beads. The solution based panning method was more efficient than conventional immobilized target panning when using whole viral particles of cymbidium mosaic virus as a target. Enzyme-linked immunosorbent assay of cymbidium mosaic virus-binding peptides isolated from the library identified seven peptides with affinity for cymbidium mosaic virus and one peptide which was specific to cymbidium mosaic virus and had no significant binding to odontoglossum ringspot virus. This method should have broad application for the screening of whole viral particles towards the rapid development of diagnostic reagents without the requirement for cloning and expression of single antigens.
Ooi
Diana Jia Miin
DJ
Biotechnology Program, Malaysia University of Science and Technology, Block C, Kelana Square, 17 Jalan SS7/26, 47301 Petaling Jaya, Selangor, Malaysia.
Dzulkurnain
Adriya
A
Othman
Rofina Yasmin
RY
Lim
Saw Hoon
SH
Harikrishna
Jennifer Ann
JA
D008024:Ligands; D008280:Magnetics; D008863:Microspheres; D009029:Mosaic Viruses; D029595:Orchidaceae / Q000821:virology; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D012680:Sensitivity and Specificity
D008024:Ligands; D019151:Peptide Library; D010455:Peptides