title: The activity and immunoexpression of cathepsin D in rat male reproductive organs.
abstract: Cathepsin D is a cysteine endopeptidase that belongs to the lysosomal enzyme family. The aim of the study was to evaluate the enzyme immunoexpression and activity in selected male genital organs in mature Wistar rats. The activity of cathepsin D was measured spectrophotometrically in homogenates of the testis, epididymis, seminal vesicle and prostate. Immunohistochemical staining was also performed in the ductus deferens. Enzyme activity was found in the following sequence: testis>epididymis>dorsal prostatic lobe>seminal vesicle>lateral prostatic lobe>ventral prostatic lobe. Although there were differences in enzyme activity between various organs of the male reproductive system, cathepsin D immunoreactivity was seen exclusively in the Sertoli and Leydig cells in the testis.
authors.affiliation: Experimental Teratology Unit of the Human Anatomy Department, Medical University of Lublin, Lublin, Poland. fb3@wp.pl
authors.forename: F
authors.initials: F
authors.lastname: Burdan
authors.forename: J
authors.initials: J
authors.lastname: Szumiło
authors.forename: J
authors.initials: J
authors.lastname: Dudka
authors.forename: A
authors.initials: A
authors.lastname: Dabrowski
authors.forename: R
authors.initials: R
authors.lastname: Maciejewski
authors.forename: Z
authors.initials: Z
authors.lastname: Wójtowicz
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism*; D004822:Epididymis / Q000166:cytology / Q000201:enzymology; D005837:Genitalia, Male / Q000166:cytology / Q000201:enzymology*; D007150:Immunohistochemistry; D007985:Leydig Cells / Q000166:cytology / Q000201:enzymology; D008297:Male; D011467:Prostate / Q000166:cytology / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D012669:Seminal Vesicles / Q000166:cytology / Q000201:enzymology; D012708:Sertoli Cells / Q000166:cytology / Q000201:enzymology; D013737:Testis / Q000166:cytology / Q000201:enzymology
chemical_list: D015415:Biomarkers; D002402:Cathepsin D