title: A dynamic role for the mushroom bodies in promoting sleep in Drosophila.
abstract: The fruitfly, Drosophila melanogaster, exhibits many of the cardinal features of sleep, yet little is known about the neural circuits governing its sleep. Here we have performed a screen of GAL4 lines expressing a temperature-sensitive synaptic blocker shibire(ts1) (ref. 2) in a range of discrete neural circuits, and assayed the amount of sleep at different temperatures. We identified three short-sleep lines at the restrictive temperature with shared expression in the mushroom bodies, a neural locus central to learning and memory. Chemical ablation of the mushroom bodies also resulted in reduced sleep. These studies highlight a central role for the mushroom bodies in sleep regulation.
authors.affiliation: Department of Neurobiology and Physiology, Northwestern University, Evanston, Ilinois 60208, USA.
authors.forename: Jena L
authors.initials: JL
authors.lastname: Pitman
authors.forename: Jermaine J
authors.initials: JJ
authors.lastname: McGill
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Keegan
authors.forename: Ravi
authors.initials: R
authors.lastname: Allada
mesh_terms: D000818:Animals; D003624:Darkness; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D005260:Female; D005786:Gene Expression Regulation; D017930:Genes, Reporter / Q000235:genetics; D006706:Homeostasis; D006918:Hydroxyurea / Q000494:pharmacology; D007858:Learning / Q000502:physiology; D008027:Light; D008297:Male; D023421:Models, Animal; D024521:Mushroom Bodies / Q000502:physiology*; D010641:Phenotype; D012890:Sleep / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D013696:Temperature; D013997:Time Factors
chemical_list: D006918:Hydroxyurea