title: Comparison of immunoblot analysis of emergent multidrug-resistant Salmonella typhimurium definitive phage type 104 with a non-multidrug-resistant definitive phage type 104 strain.
abstract: OBJECTIVE
  The emergent multidrug-resistant (MDR) Salmonella typhimurium definitive phage type 104 (DT104) is a public and veterinary health problem not only due to its wide host range and potential for enhanced virulence, but also the difficulty associated with its control. There is thus a need to investigate possible antigens of MDR DT104.
  
  
  METHODS
  Using standard protocols, whole cell lysates, outer membrane extracts and cell-free ultracentrifuge supernatants of selected isolates of MDR DT104 were prepared, electrophoretically separated and tested for their antigen-antibody reactivity in comparison with a non-MDR DT104 strain.
  
  
  RESULTS
  Protein antigens of both strain types were recognized by antibodies in chick serum in a similar manner for all methods of antigen preparation used.
  
  
  CONCLUSIONS
  This study did not find differences between the antibody recognition of MDR DT104 and that of the non-MDR DT104 strain tested. This observation should strengthen the quest for the possible use of vaccines to control this emergent strain in poultry.
authors.affiliation: Department of Microbiology, Human Virology and Biotechnology, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria. ybngwai@yahoo.com
authors.forename: Yakubu B
authors.initials: YB
authors.lastname: Ngwai
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Adachi
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Kolo
authors.initials: K
authors.lastname: Ibrahim
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002645:Chickens; D003429:Cross Reactions; D018432:Drug Resistance, Multiple; D019450:Genes, MDR; D006801:Humans; D015151:Immunoblotting; D008826:Microbial Sensitivity Tests; D011817:Rabbits; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014461:Ultracentrifugation
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins