Lactococcus lactis GEM particles displaying pneumococcal antigens induce local and systemic immune responses following intranasal immunization.
The present work reports the use of non-living non-recombinant bacteria as a delivery system for mucosal vaccination. Antigens are bound to the cell-wall of pretreated Lactococcus lactis, designated as Gram-positive enhancer matrix (GEM), by means of a peptidoglycan binding domain. The influence of the GEM particles on the antigen-specific serum antibody response was studied. Following nasal immunization with the GEM-based vaccines, antibody responses were induced at systemic and local levels. Furthermore, different GEM-based vaccines could be used consecutively in the same mice without adverse effects or loss of activity. Taken together, the results evidence the adjuvant properties of the GEM particles and indicate that GEM-based vaccines can be used repeatedly and are particularly suitable for nasal immunization purposes.
Audouy
Sandrine A L
SA
BiOMaDe Technology, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
van Roosmalen
Maarten L
ML
Neef
Jolanda
J
Kanninga
Rolf
R
Post
Eduard
E
van Deemter
Mariëlle
M
Metselaar
Heidi
H
van Selm
Saskia
S
Robillard
George T
GT
Leenhouts
Kees J
KJ
Hermans
Peter W M
PW
D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D013294:Lactococcus lactis / Q000378:metabolism*; D051379:Mice; D010957:Plasmids; D022242:Pneumococcal Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D013296:Streptococcus pneumoniae / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D022242:Pneumococcal Vaccines