Influence of water miscible organic solvents on alpha-chymotrypsin in solution and immobilized on Eupergit CM.
The effects of the water-miscible organic solvents (methanol, ethanol, 1-propanol, 2-propanol, acetonitrile, N,N'-dimethylformamide and tetrahydrofuran) on the stability and catalytic activity of alpha-chymotrypsin (CT) immobilized on Eupergit CM were studied. Enhanced stabilities and activities were observed both as a consequence of immobilization and the presence of organic solvent, which in combination provide long term (at least 24 h) retention of activity, and up to 50-fold increase in 50% (v/v) methanol in buffer. Low quantities (20%, v/v) of acetonitrile not only prevented CT inactivation by autolysis at 20 degrees C but also induced a significant increase in the activity of both free (six-fold) and immobilized (two-fold) CT.
Olofsson
Linus
L
Department of Chemistry & Biomedical Sciences, University of Kalmar, SE-391 82, Kalmar, Sweden.
Söderberg
Pernilla
P
Nicholls
Ian A
IA
D000097:Acetonitriles / Q000494:pharmacology*; D002918:Chymotrypsin / Q000737:chemistry*; D004126:Dimethylformamide / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry*; D007700:Kinetics; D011108:Polymers; D012997:Solvents / Q000737:chemistry*
D000097:Acetonitriles; D004800:Enzymes, Immobilized; D011108:Polymers; D012997:Solvents; D000431:Ethanol; D004126:Dimethylformamide; C065843:Eupergit C; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; C032159:acetonitrile