Isolation of marine bacteria by in situ culture on media-supplemented polyurethane foam.
Polyurethane foam (PUF) supplemented with various agar media was used in situ to trap marine bacteria and it consequently provided a substrate on which they could be cultivated while exposed to natural seawater in the coral reef area. The bacterial population on the PUF blocks was analyzed by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 16S rDNA fragments. Changing the composition of the cultivation medium in the PUF blocks and selecting different sampling sites resulted in different bacteria being detected on the PUF blocks. For example, iron-utilizing (IU) bacteria, siderophore-producing (SP) bacteria, and petroleum-degrading (PD) bacteria were isolated from PUF blocks and it was discovered that IU and SP contained iron and PD contained hydrocarbon. This method opens up the possibility for isolating novel and useful marine bacteria.
Yasumoto-Hirose
Mina
M
Marine Biotechnology Institute (MBI), 3-75-1 Heita, Kamaishi-shi, Iwate, 026-0001, Japan.
Nishijima
Miyuki
M
Ngirchechol
Metiek Kimie
MK
Kanoh
Kaneo
K
Shizuri
Yoshikazu
Y
Miki
Wataru
W
D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D001431:Bacteriological Techniques; D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D010578:Petroleum; D011140:Polyurethanes / Q000737:chemistry*; D012623:Seawater; D017262:Siderophores / Q000378:metabolism
D003470:Culture Media; D010578:Petroleum; D011140:Polyurethanes; D017262:Siderophores