Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding.
The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described.
Sanchez-Weatherby
Juan
J
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, England. j.sanchez-weatherby@uea.ac.uk
Southall
Stacey
S
Oubrie
Arthur
A
D016954:Acinetobacter calcoaceticus / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D005948:Glucose Dehydrogenases / Q000737:chemistry*; D008320:Maltose / Q000378:metabolism; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D008320:Maltose; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone)