Identification and characterization of D-AKAP1 as a major adipocyte PKA and PP1 binding protein.
Protein kinase A (PKA) plays an important role in the regulation of lipid metabolism in adipocytes. The activity of PKA is known to be modulated by its specific location in the cell, a process mediated by A-kinase anchoring proteins (AKAPs). In order to examine the subcellular localization of PKA in this tissue we performed a search for AKAP proteins in adipocytes. We purified a 120 kDa protein which can bind both the regulatory subunit of PKA as well as the catalytic subunit of protein phosphatase 1 (PP1). This protein was found to be enriched in the lipid droplet fraction of primary adipocytes and was identified as D-AKAP1. This protein may play an important role in the regulation of PKA in adipocytes.
Bridges
Dave
D
Department of Biological Sciences, University of Calgary, Calgary, Alta., Canada T2N 4N1.
MacDonald
Justin A
JA
Wadzinski
Brian
B
Moorhead
Greg B G
GB
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing / Q000302:isolation & purification* / Q000378:metabolism*; D017667:Adipocytes / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D008297:Male; D015388:Organelles / Q000737:chemistry; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D054645:Protein Phosphatase 1; D051381:Rats
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; C486332:Akap1 protein, rat; D017868:Cyclic AMP-Dependent Protein Kinases; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1