[Expression of HIC-5/ARA55 in colonrectal cancer and its mechanisms of action].
OBJECTIVE
To investigate the relationship between HIC-5 and colonrectal cancer.


METHODS
Samples were obtained from 42 patients with colonrectal cancer, and the mRNA and protein expressions of HIC-5/ARA55 were determined by RT-PCR and Western-blot respectively. FCM was used to investigate the apoptosis of Lovo in the HIC-5/ARA55 transfection cells. The expression of apoptosis related proteins Bax and Bcl-2 was determined by Western-blot, and the MTT assay was used to test cell growth speed affected by HIC-5/ARA55.


RESULTS
The expression of HIC-5 significantly decreased on colonrectal cancer tissues as compared with adjacent normal tissues on mRNA level and protein level(P<0.05). The apoptosis rate increase significantly in the HIC-5/ARA55 transfected cells. HIC-5/ARA55 could restrain tumor cell grow speed.


CONCLUSIONS
HIC-5/ARA55 is the protein that can reduce tumor cell growth rate, and induce apoptosis in colonrectal cancer cells.
Cui
Wei
W
Department of General Surgery, Peking University First Hospital, BeiJing 100034, China.
Wang
Xin
X
Liu
Yu-cun
YC
Wan
Yuan-lian
YL
Guo
Hong-jie
HJ
Zhu
Jing
J
D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D060588:LIM Domain Proteins; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D012333:RNA, Messenger; C490351:TGFB1I1 protein, human; D016212:Transforming Growth Factor beta