Antigenic profile of osteoblasts present in human bone tissue sections.
The antigenic profile of human osteoblasts was previously analyzed by our group using primary cultures as study samples. These studies suggested a novel functional approach to this cell population. Osteoblasts have a characteristic antigenic profile and share antigens in common with other cell populations that also originate in the bone marrow. Some of the detected antigens are constitutively expressed, while others are modulated by different factors and/or cytokines. The aim of the present study was to analyze the antigens present in osteoblasts in vivo, since the presence of certain biomolecules in fetal bovine serum may modulate the antigenic expression, compromising the results. For this purpose, human bone tissue sections were analyzed with a wide panel of mAbs and using the immunoperoxidase technique. CD10, CD44 and alkaline phosphatase antigens and IL-12, IL-18 and IFNgamma cytokines were detected in osteoblasts in the bone tissue. However, CD80 and HLA-DR antigens were not found in all samples and when present their expression was weak. The expression of CD54 antigen was moderate or weak. These results allow data obtained by the primary culture of osteoblast-like cells to be endorsed.
García-Martínez
O
O
Department of Nursing, Physiology Section, University of Granada, E-18012 Granada, Spain.
Reyes-Botella
C
C
Aguilera-Castillo
O
O
Vallecillo-Capilla
M F
MF
Ruiz
C
C
D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology; D001842:Bone and Bones / Q000166:cytology*; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology*; D007124:Immunoenzyme Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism; D020382:Interleukin-18 / Q000235:genetics / Q000378:metabolism; D008297:Male; D008867:Microtomy / Q000379:methods; D015260:Neprilysin / Q000276:immunology*; D010006:Osteoblasts / Q000166:cytology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D018960:Hyaluronan Receptors; D020382:Interleukin-18; D018799:Intercellular Adhesion Molecule-1; D018664:Interleukin-12; D007371:Interferon-gamma; D000469:Alkaline Phosphatase; D015260:Neprilysin