Assessment of bone structure and acoustic impedance in C3H and BL6 mice using high resolution scanning acoustic microscopy.
Two hundred-MHz time-resolved scanning acoustic microscopy was applied for the investigation of acoustic and structural bone properties of mice from two inbred strains. Transverse sections of femur taken from 5 C57BL/6J@Ico and 5 C3H/HeJ@Ico mice were explored. Both strains had the same bone diameter, but the C3H/HeJ@Ico mice had greater cortical thickness, smaller cancellous diameter, and greater acoustic impedance values than C57BL/6J@Ico mice. The strong differences in the measured acoustic impedances among the two inbred strains indicate that the impedance is a good parameter to detect genetic variations of the skeletal phenotype in small animal models.
Hofman
Tobias
T
Laboratoire d'Imagerie Paramétrique, CNRS/Université Paris 6, UMR 7623, 15, Paris, France.
Raum
Kay
K
Leguerney
Ingrid
I
Saïed
Amena
A
Peyrin
Françoise
F
Vico
Laurence
L
Laugier
Pascal
P
D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology*; D017097:Electric Impedance; D005269:Femur / Q000000981:diagnostic imaging* / Q000502:physiology*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D033401:Microscopy, Acoustic / Q000379:methods*