Amino acids 15-28 in the ectodomain of SARS coronavirus 3a protein induces neutralizing antibodies.
A synthetic peptide corresponding to amino acids (aa) 15-28 of the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein was used to raise polyclonal antibodies in rabbits. This anti-3a N-terminal antibody could detect 3a protein in infected cells, as did an anti-3a C-terminal antibody previously described. The latter targeted the C-terminal cytoplasmic domain of 3a (aa 134-274). The anti-3a N-terminal antibody could detect intracellular 3a as well as 3a expressed on the cell surface. Interestingly, only the anti-3a N-terminal antibody can inhibit SARS-CoV propagation in Vero E6 culture although the binding affinity of the anti-3a N-terminal antibody was lower than the anti-3a C-terminal antibody.
Akerström
Sara
S
Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden.
Tan
Yee-Joo
YJ
Mirazimi
Ali
A
D000596:Amino Acids / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D001666:Binding Sites, Antibody; D019556:COS Cells; D002522:Chlorocebus aethiops; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006801:Humans; D009500:Neutralization Tests; D017434:Protein Structure, Tertiary; D011817:Rabbits; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000737:chemistry* / Q000276:immunology*; D000086082:Viroporin Proteins
C487105:3a protein, SARS-CoV; D000596:Amino Acids; D000914:Antibodies, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D000086082:Viroporin Proteins