Development of resistant transgenic soybeans with inverted repeat-coat protein genes of soybean dwarf virus.
In an attempt to generate soybean plants resistant to soybean dwarf virus (SbDV), we transformed a construct containing inverted repeat-SbDV coat protein (CP) genes spaced by beta-glucuronidase (GUS) sequences into soybean somatic embryos via microprojectile bombardment. Three T(0) plants with an introduced CP gene were obtained, and one generated T(1) seeds. The presence of the transgene in T(1) plants was confirmed by PCR and Southern blot hybridization analysis, but expression of CP was not detected by northern blot hybridization analysis. Two months after inoculation of SbDV by aphid, T(2) plants contained little SbDV-specific RNA and remained symptomless. These plants contained SbDV-CP-specific siRNA. These results suggest that the T(2) plants achieved resistance to SbDV by an RNA-silencing-mediated process.
Tougou
Makoto
M
National Agricultural Research Center for Tohoku Region, Morioka, Iwate, 020-0198, Japan.
Furutani
Noriyuki
N
Yamagishi
Noriko
N
Shizukawa
Yoshiaki
Y
Takahata
Yoshihito
Y
Hidaka
Soh
S
D036022:Capsid Proteins / Q000235:genetics*; D017793:Luteovirus / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000821:virology; D034622:RNA Interference; D012367:RNA, Viral / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D012639:Seeds / Q000235:genetics / Q000821:virology; D013025:Glycine max / Q000235:genetics* / Q000821:virology; D014170:Transformation, Genetic
D036022:Capsid Proteins; D012367:RNA, Viral; C118138:coat protein, Soybean dwarf virus