Inhibition of proliferation and induction of apoptosis by EGCG in human osteogenic sarcoma (HOS) cells.
EGCG [(-)-epigallocatechin-3-gallate], a major component of green tea has been considered as a major antioxidant constituent. In addition to having been considered for cancer treatment as a chemopreventive and chemotherapeutic agent, EGCG has recently been attributed an anti-proliferative effect. We re-examined the latter finding in this study and added specific focus on the ability of EGCG to induce apoptosis in human osteogenic sarcoma (HOS) cells. Antiproliferative action of EGCG (IC50 = 35.3 +/- 6.0 microg/mL) appeared to be linked to apoptotic cell death based on morphological changes, chromosomal DNA degradation, and an increase in the sub-G1 apoptotic cell population. Treatment of HOS cells with EGCG gradually activated caspase-3, an established inducer of apoptotic cell death.
Ji
Sang Jin
SJ
Department of Biochemistry, College of Dentistry, KyungHee University, Seoul 130-701, Korea.
Han
Dong Hoon
DH
Kim
Jeong Hee
JH
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D016923:Cell Death; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*
D000970:Antineoplastic Agents; D002392:Catechin; C045651:epigallocatechin gallate; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases