title: Effective PCR detection of animal species in highly processed animal byproducts and compound feeds.
abstract: In this paper we present a polymerase chain reaction (PCR)-based method for detecting meat and bone meal (MBM) in compound feedingstuffs. By choosing adequate DNA targets from an appropriate localisation in the genome, the real-time PCR method developed here proved to be robust to severe heat treatment of the MBM, showing high sensitivity in the detection of MBM. The method developed here permits the specific detection of processed pig and cattle materials treated at 134 degrees C in various feed matrices down to a limit of detection of about 0.1%. This technique has also been successfully applied to well-characterised MBM samples heated to as high as 141 degrees C, as well as to various blind feed samples with very low MBM contents. Finally, the method also passed several official European ring trials.
authors.affiliation: Département Qualité des Productions Agricoles, Centre Wallon de Recherches agronomiques (CRA-W), 24 Chaussée de Namur, 5030 Gembloux, Belgium. fumiere@cra.wallonie.be
authors.forename: Olivier
authors.initials: O
authors.lastname: Fumière
authors.forename: Marc
authors.initials: M
authors.lastname: Dubois
authors.forename: Vincent
authors.initials: V
authors.lastname: Baeten
authors.forename: Christoph
authors.initials: C
authors.lastname: von Holst
authors.forename: Gilbert
authors.initials: G
authors.lastname: Berben
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000829:Animals, Domestic; D001688:Biological Products / Q000032:analysis; D002417:Cattle; D008460:Meat / Q000032:analysis*; D008903:Minerals / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D013552:Swine
chemical_list: D001688:Biological Products; D008903:Minerals; C027762:bone meal