The hemagglutinating activity of Pseudomonas aeruginosa strains isolated from humans and animals.
The presence and type of adhesins occurring in Pseudomonas aeruginosa strains were determined by hemagglutination test with a 3% suspension of normal and trypsin-treated human group A erythrocytes, with or without the addition of sugar inhibitors (D-mannose, D-glucose, L-fucose, D-galactose, D-fructose, lactose, N-acetylneuraminic acid, N-acetylglucosamine and N-acetylgalactosamine). This study showed that a low percentage of Pseudomonas aeruginosa strains caused the agglutination of normal erythrocytes. Trypsin treatment of erythrocytes did not affect the hemagglutinating properties, indicating that hemagglutination was not dependent upon a trypsin-sensitive protein on the erythrocytes surface. Most of the studied strains agglutinated RBCs at 37 degrees C. A great variability in the inhibiting activity on studied strains was observed among the carbohydrates tested. These results demonstrated the predominant role of N-acetylglucosamine, N-acetylgalactosamine and N-acetylneuraminic acid for Pseudomonas aeruginosa adhesion to RBCs.
Wolska
K
K
Department of Microbiology, Agricultural Faculty, University of Podlasie, Prusa 12, 08-110 Siedlce, Poland. kwolska@ap.siedlce.pl
Swiderska
E
E
Jakubczak
A
A
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D004912:Erythrocytes / Q000502:physiology; D006384:Hemagglutination / Q000502:physiology*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000502:physiology*