Determination of constituents of Telazol--tiletamine and zolazepam by a gas chromatography/mass spectrometry-based method.
Tiletamine and zolazepam injection (Telazol) is used in veterinary surgical practice to induce short-term anesthesia and also to immobilize wild animals. The present work describes a sensitive method to measure tiletamine and zolazepam concentrations in plasma by means of GC/EI-MS on a 5% phenyl/95% methylpolysiloxane column. A simple liquid extraction procedure with ethyl acetate was used to isolate the two compounds and the same were separated and analyzed by GC/MS without derivatization. A formal validation of the assay demonstrated good accuracy and precision for both tiletamine (98-100.8%; C.V.total < 6.7%) and zolazepam (98.3-103.4; C.V.total < 13.2%). With 500 microl of plasma, the limits of quantification for both tiletamine and zolazepam were found to be 10 ng/ml. Both compounds were stable after three freeze-thaw cycles. The assay was used to analyze plasma samples collected from a pig after intramuscular administration of 10 mg/kg of Telazol. The plasma concentration-time profile of tiletamine and zolazepam from this representative pig is also provided.
Kumar
Atul
A
Department of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, 308 Harvard St., SE, Minneapolis, MN 55414, USA.
Mann
Henry J
HJ
Remmel
Rory P
RP
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D013552:Swine; D013992:Tiletamine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D015041:Zolazepam / Q000032:analysis* / Q000097:blood / Q000737:chemistry
D013992:Tiletamine; D015041:Zolazepam