FSH receptor gene polymorphisms have a role for different ovarian response to stimulation in patients entering IVF/ICSI-ET programs.
OBJECTIVE
To examine the frequency distribution of the Ser680Asn polymorphism of the follicle-stimulating hormone receptor (FSHR) gene in ovarian dysfunction (OD) infertile women, "poor responders" (PR) and "good responders" (GR).


METHODS
The hormonal profiles and treatment of all patients were analyzed and FSHR polymorphism was examined by PCR and RFLP. Women from all groups were classified as Asn/Asn, Asn/Ser, and Ser/Ser genotypes.


RESULTS
The frequency distribution of Ser/Ser, Asn/Ser and Asn/Asn variants in OD patients was 45.5, 22.7, and 31.8%, respectively. Day 3 FSH levels in OD and GR patients were higher in Ser/Ser and Asn/Asn subgroups. Asn/Ser carriers from OD and GR groups provided more follicles and oocytes compared to other allelic variants.


CONCLUSIONS
GR patients carry more often the Asn/Ser genotype. The latter is correlated with more follicles and oocytes in both OD and GR patients. The Ser/Ser variant might be related to higher serum FSH levels, while the Asn/Ser with lower.
Loutradis
Dimitris
D
Division of Human Reproduction, IVF Unit, 1st Department of Obstetrics and Gynaecology, Athens University Medical School, Athens, Greece.
Patsoula
Eleni
E
Minas
Vassilis
V
Koussidis
Giorgos A
GA
Antsaklis
Aristeidis
A
Michalas
Stylianos
S
Makrigiannakis
Antonis
A
D000328:Adult; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood; D010053:Ovary / Q000502:physiology*; D010062:Ovulation Induction; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D016649:Primary Ovarian Insufficiency / Q000235:genetics; D011962:Receptors, FSH / Q000097:blood / Q000235:genetics*
D011962:Receptors, FSH; D007986:Luteinizing Hormone