The daily rhythms of melatonin and free fatty acids in goats under varying photoperiods and constant darkness.
The purpose of the study was to explore parallel and divergent features of the daily rhythms of melatonin and plasma free fatty acids (FFA) in goats exposed to different lighting conditions. From these features, we attempted to analyze whether the endogenous melatonin rhythm plays any role in the maintenance of the FFA rhythm. Seven Finnish landrace goats were kept under artificial lighting that simulated the annual changes of photoperiod at 60 degrees N (longest photoperiod, 18 h; shortest, 6 h). The ambient temperature and feeding regimen were kept constant. Blood samples were collected 6 times a year at 2 h intervals for 2 d, first in the prevailing light-dark (LD) conditions and then after 3 d in constant darkness (DD). In LD conditions, the melatonin levels always increased immediately after lights-off and declined around lights-on, except in winter (18 h darkness), when the low daytime levels were restored clearly before lights-on. The FFA levels also displayed a consistent rhythmicity, with low levels at night and a transient peak around lights-on. In DD conditions, the melatonin profiles were very similar to those found in the habitual LD conditions, but the rhythm tended to advance. The FFA rhythm persisted also in DD, and the morning peak tended to advance. There was an overall parallelism between the two rhythms, with one significant exception. In winter in LD conditions, the morning rise in FFA levels coincided with lights-on and not with the declining phase of melatonin, whereas in DD conditions, the FFA peak advanced several hours and coincided with the declining phase of melatonin. From this finding and comparisons of the calculated rhythm characteristics, i.e., phase-shifts, phase differences, and correlations, we conclude that the daily rhythm of FFA levels is most probably generated by an endogenous oscillator, primarily adjusted by dawn, whereas the melatonin rhythm in this species is regulated by an oscillator primarily adjusted by dusk. The results did not exclude a modulatory effect of melatonin on the daily FFA profiles, but melatonin secretion, alone, does not explain the patterns sufficiently.
Alila-Johansson
Aino
A
Department of Basic Veterinary Sciences, University of Helsinki, Helsinki, Finland. aino.alila-johansson@helsinki.fi
Eriksson
Lea
L
Soveri
Timo
T
Laakso
Maija-Liisa
ML
D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D003624:Darkness; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D006041:Goats / Q000097:blood / Q000502:physiology*; D008550:Melatonin / Q000097:blood*; D017440:Photoperiod
D005230:Fatty Acids, Nonesterified; D008550:Melatonin