[Protein-protein interaction between ESAT-6 and CFP-10 of mycobacterium tuberculosis].
OBJECTIVE
To investigate the protein-protein interaction between ESAT-6 and CFP-10 of mycobacterium tuberculosis.


METHODS
ESAT-6 gene and CFP-10 gene were amplified from mycobacterium tuberculosis genome DNA. The ESAT-6 gene was subcloned into pGBKT7 and the CFP-10 gene was subcloned into pGADT7-Rec. After being verified with restriction endonuclease digestion and DNA sequencing, the recombinant vectors were transformed into yeast cell AH109 by lithium acetate method.


RESULTS
The yeast cells co-transformed with pGBKT7-ESAT-6 and pGADT7-CFP-10 grew on SD/-Ade/-His/-Leu/-Trp plates, and beta-galactosidase activity assays showed positive results.


CONCLUSIONS
ESAT-6 and CFP-10 protein could interact with each other in yeast cells.
Li
Yan
Y
Research Unit of Infection and Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Bao
Lang
L
Zhang
Hui-dong
HD
Wang
Xiao-ying
XY
Zhu
Hai-long
HL
Li
Ya-sha
YS
D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020798:Two-Hybrid System Techniques
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; D004269:DNA, Bacterial; C093276:ESAT-6 protein, Mycobacterium tuberculosis