title: Microbial hydrogen production with Bacillus coagulans IIT-BT S1 isolated from anaerobic sewage sludge.
abstract: Bacillus coagulans strain IIT-BT S1 isolated from anaerobically digested activated sewage sludge was investigated for its ability to produce H(2) from glucose-based medium under the influence of different environmental parameters. At mid-exponential phase of cell growth, H(2) production initiated and reached maximum production rate in the stationary phase. The maximal H(2) yield (2.28 mol H(2)/molglucose) was recorded at an initial glucose concentration of 2% (w/v), pH 6.5, temperature 37 degrees C, inoculum volume of 10% (v/v) and inoculum age of 14 h. Cell growth rate and rate of hydrogen production decreased when glucose concentration was elevated above 2% w/v, indicating substrate inhibition. The ability of the organism to utilize various carbon sources for H(2) fermentation was also determined.
authors.affiliation: Fermentation Technology Laboratory, Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.
authors.forename: Shireen Meher
authors.initials: SM
authors.lastname: Kotay
authors.forename: Debabrata
authors.initials: D
authors.lastname: Das
mesh_terms: D000693:Anaerobiosis; D001407:Bacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D006859:Hydrogen; D005947:Glucose