Gene rearrangements in follicular lymphoma among Indonesian.
OBJECTIVE
Gene rearrangement has an important role in the management of lymphoma. We investigated the rearrangements of B-cell leukaemia/lymphoma 2 (BCL2), BCL6 and Paired homeobox 5 (PAX5) genes in Indonesian follicular lymphoma (FL) patients.


METHODS
We examined gene rearrangements using various kinds of polymerase chain reactions (PCRs) on 24 patients' peripheral blood DNA.


RESULTS
BCL2 rearrangement was found in 58% (14 of 24 patients), 8 at mbr (major breakpoint region), 2 at mcr (minor cluster region) and 4 at icr (intermediate cluster region), respectively. No rearrangement in BCL6 and PAX5 was detected. There was a significant difference in the incidence of spleen involvement between patients with BCL-2 rearrangement and without it (50% vs. 11%, p=0.04). BCL-2 rearrangement was correlated with spleen involvement (OR=9) and anemia (OR=2.3).


CONCLUSIONS
BCL2 rearrangement in Indonesian FL was higher than previous reports from other Asia countries (58% vs. 48%, respectively). Our method using peripheral blood DNA might be useful for the molecular diagnosis of FL.
Oehadian
Amaylia
A
Division of Hematology and Medical Oncology, Department of Internal medicine, Padjadjaran University, Hasan Sadikin Hospital, Bandung, Indonesia.
Koide
Naoki
N
Fianza
Pandji Irani
PI
Fadjari
Trinugroho Heri
TH
Sumantri
Rachmat
R
Supandiman
Iman
I
Yokochi
Takashi
T
D000740:Anemia / Q000453:epidemiology; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D015321:Gene Rearrangement; D019254:Genes, bcl-2 / Q000235:genetics; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D008224:Lymphoma, Follicular / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D051757:PAX5 Transcription Factor / Q000235:genetics; D016133:Polymerase Chain Reaction; D051560:Proto-Oncogene Proteins c-bcl-6; D013160:Splenic Neoplasms / Q000453:epidemiology; D014178:Translocation, Genetic
C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; D051560:Proto-Oncogene Proteins c-bcl-6