A normalised scale for structural genomics target ranking: the OB-Score.
Target selection and ranking is fundamental to structural genomics. We present a Z-score scale, the "OB-Score", to rank potential targets by their predicted propensity to produce diffraction-quality crystals. The OB-Score is derived from a matrix of predicted isoelectric point and hydrophobicity values for nonredundant PDB entries solved to <or=3.0 A against a background of UniRef50. A highly significant difference was found between the OB-Scores for TargetDB test datasets. A wide range of OB-Scores was observed across 241 proteomes and within 7868 PfamA families; 73.4% of PfamA families contain >or=1 member with a high OB-Score, presenting favourable candidates for structural studies.
Overton
Ian M
IM
School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.
Barton
Geoffrey J
GJ
D030562:Databases, Protein; D023281:Genomics / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D007526:Isoelectric Point; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D013223:Statistics as Topic
D011506:Proteins