Methods for studying the cellular response to DNA damage: influence of the Mre11 complex on chromosome metabolism.
Dramatic progress in understanding the mediators and mechanisms of chromosome break metabolism has been made in recent years. As a result, the links between disease and defects in chromosome dynamics have become clearer. In this chapter, we discuss techniques employed in our laboratory to study chromosome break metabolism, which include assessments at the molecular and cellular level. In our laboratory, we use the Mre11 complex as a tool to study this process, but the techniques discussed are of general relevance.
Theunissen
Jan-Willem F
JW
Memorial Sloan Kettering Cancer Center, New York, New York, USA.
Petrini
John H J
JH
D000818:Animals; D002453:Cell Cycle; D002461:Cell Line, Transformed; D002875:Chromosomes; D004249:DNA Damage; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins / Q000235:genetics*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D000076228:MRE11 Homologue Protein; D051379:Mice; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
D004268:DNA-Binding Proteins; C121766:MRE11 protein, human; C512689:Mre11a protein, mouse; D000076228:MRE11 Homologue Protein; D045643:DNA Repair Enzymes