title: Gingival crevicular fluid transforming growth factor-beta1 in several forms of periodontal disease.
abstract: BACKGROUND
  Transforming growth factor-beta(1) (TGF-beta(1)) has significant effects on periodontal host response regulation. Limited knowledge on the role of TGF-beta(1) in various periodontal disease types and particularly in advanced periodontitis forms warranted the present study. The aim of the present study was to evaluate the gingival crevicular fluid (GCF) TGF-beta(1) levels in patients with different forms of periodontal disease.
  
  
  METHODS
  GCF TGF-beta(1) levels were investigated in 32 chronic periodontitis (CP), 30 generalized aggressive periodontitis (G-AgP), 15 gingivitis patients and 16 periodontally healthy subjects. Periodontal status was evaluated by measuring probing depth, clinical attachment loss, plaque and bleeding on probing. TGF-beta(1) levels were analyzed by enzyme-linked immunosorbent assay. The results were expressed in terms of total amount (pg) and concentration (pg/microl).
  
  
  RESULTS
  G-AgP and CP groups had significantly elevated GCF TGF-beta(1) total amount compared to healthy group (p<0.008). Moreover, GCF TGF-beta(1) total amount of G-AgP group was significantly higher than that of gingivitis group (p<0.008). G-AgP and CP groups had similar GCF TGF-beta(1) total amount (p>0.008). Significant correlation was found between GCF TGF-beta(1) total amount and all clinical periodontal parameters (p<0.05).
  
  
  CONCLUSIONS
  The results of the present study suggest contribution of TGF-beta(1) to the pathogenesis of advanced chronic and aggressive periodontitis. TGF-beta(1) may thus be one of the components modulating exaggerated host response together with other major mediators of inflammation.
authors.affiliation: Ege University, School of Dentistry, Department of Periodontology, Bornova-35100, Izmir, Turkey. aligurkan00@yahoo.com
authors.forename: Ali
authors.initials: A
authors.lastname: Gürkan
authors.forename: Gülnur
authors.initials: G
authors.lastname: Emingil
authors.forename: Serhat
authors.initials: S
authors.lastname: Cinarcik
authors.forename: Afig
authors.initials: A
authors.lastname: Berdeli
mesh_terms: D000328:Adult; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005891:Gingivitis / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010510:Periodontal Diseases / Q000378:metabolism*; D010518:Periodontitis / Q000378:metabolism; D012720:Severity of Illness Index; D013048:Specimen Handling / Q000379:methods; D053773:Transforming Growth Factor beta1 / Q000032:analysis*
chemical_list: D053773:Transforming Growth Factor beta1