title: Over-production of beta-carotene from metabolically engineered Escherichia coli.
abstract: To produce recombinant beta-carotene in vitro, synthetic operons encoding genes governing its biosynthesis were engineered into Escherichia coli. Constructs harboring these operons were introduced into either a high-copy or low-copy cloning vector. beta-Carotene production from these recombinant E. coli cells was either constitutive or inducible depending upon plasmid copy number. The most efficient beta-carotene production was with the low-copy based vector. The process was increased incrementally from a 5 l to a 50 l fermentor and finally into a 300 l fermentor. The maximal beta-carotene yields achieved using the 50 l and 300 l fermentor were 390 mg l(-1) and 240 mg l(-1), respectively, with overall productivities of 7.8 mg l(-1) h(-1) and 4.8 mg l(-1) h(-1).
authors.affiliation: AceBiotech Co Ltd, No 114 Bio-Venture Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Jae-Bum
authors.initials: JB
authors.lastname: Kim
authors.forename: W-H
authors.initials: WH
authors.lastname: Jung
authors.forename: Jung-Hoe
authors.initials: JH
authors.lastname: Kim
authors.forename: Joon-Ki
authors.initials: JK
authors.lastname: Jung
mesh_terms: D004345:Drug Industry / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D009876:Operon / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism*; D019207:beta Carotene / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D019207:beta Carotene