title: Gene rearrangements in follicular lymphoma among Indonesian.
abstract: OBJECTIVE
  Gene rearrangement has an important role in the management of lymphoma. We investigated the rearrangements of B-cell leukaemia/lymphoma 2 (BCL2), BCL6 and Paired homeobox 5 (PAX5) genes in Indonesian follicular lymphoma (FL) patients.
  
  
  METHODS
  We examined gene rearrangements using various kinds of polymerase chain reactions (PCRs) on 24 patients' peripheral blood DNA.
  
  
  RESULTS
  BCL2 rearrangement was found in 58% (14 of 24 patients), 8 at mbr (major breakpoint region), 2 at mcr (minor cluster region) and 4 at icr (intermediate cluster region), respectively. No rearrangement in BCL6 and PAX5 was detected. There was a significant difference in the incidence of spleen involvement between patients with BCL-2 rearrangement and without it (50% vs. 11%, p=0.04). BCL-2 rearrangement was correlated with spleen involvement (OR=9) and anemia (OR=2.3).
  
  
  CONCLUSIONS
  BCL2 rearrangement in Indonesian FL was higher than previous reports from other Asia countries (58% vs. 48%, respectively). Our method using peripheral blood DNA might be useful for the molecular diagnosis of FL.
authors.affiliation: Division of Hematology and Medical Oncology, Department of Internal medicine, Padjadjaran University, Hasan Sadikin Hospital, Bandung, Indonesia.
authors.forename: Amaylia
authors.initials: A
authors.lastname: Oehadian
authors.forename: Naoki
authors.initials: N
authors.lastname: Koide
authors.forename: Pandji Irani
authors.initials: PI
authors.lastname: Fianza
authors.forename: Trinugroho Heri
authors.initials: TH
authors.lastname: Fadjari
authors.forename: Rachmat
authors.initials: R
authors.lastname: Sumantri
authors.forename: Iman
authors.initials: I
authors.lastname: Supandiman
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokochi
mesh_terms: D000740:Anemia / Q000453:epidemiology; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D015321:Gene Rearrangement; D019254:Genes, bcl-2 / Q000235:genetics; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D008224:Lymphoma, Follicular / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D051757:PAX5 Transcription Factor / Q000235:genetics; D016133:Polymerase Chain Reaction; D051560:Proto-Oncogene Proteins c-bcl-6; D013160:Splenic Neoplasms / Q000453:epidemiology; D014178:Translocation, Genetic
chemical_list: C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; D051560:Proto-Oncogene Proteins c-bcl-6