Voltammetric determination of tyrosine based on an L-serine polymer film electrode.
A novel film electrode for the voltammetric determination of tyrosine has been constructed based on electropolymerization of L-serine on a glassy carbon electrode. Voltammetric behaviour of tyrosine on the poly-L-serine film electrode was investigated with cyclic voltammetry and linear sweep voltammetry, and electrochemical parameters were calculated from chronocoulometry. In optimal conditions, the oxidation peak current of tyrosine on the poly-L-serine film electrode was enhanced greatly. A sensitive oxidation peak at 0.90 V was employed to determine tyrosine. A linearity between the oxidation peak current and the tyrosine concentration was obtained in the range of 3.0 x 10(-7) to 1.0 x 10(-4) mol L(-1) with a detection limit of 1.0 x 10(-7)mol L(-1). The practical application of the film electrode in the determination of tyrosine in a commercial amino acid oral solution demonstrated that it has good selectivity and high sensitivity.
Li
Chunya
C
College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China. lcychem@yahoo.com
D000596:Amino Acids / Q000032:analysis; D002244:Carbon; D004563:Electrochemistry; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D010455:Peptides; D011506:Proteins / Q000737:chemistry; D012996:Solutions; D014443:Tyrosine / Q000032:analysis* / Q000737:chemistry
D000596:Amino Acids; D010455:Peptides; D011506:Proteins; D012996:Solutions; C027794:polyserine; D014443:Tyrosine; D002244:Carbon