New restriction fragment length polymorphisms in the cytochrome oxidase I gene facilitate host strain identification of fall armyworm (Lepidoptera: Noctuidae) populations in the southeastern United States.
Several restriction sites in the cytochrome oxidase I gene of fall armyworm, Spodoptera frugiperda (J.E. Smith), were identified by sequence analysis as potentially being specific to one of the two host strains. Strain specificity was demonstrated for populations in Florida, Texas, Mississippi, Georgia, and North Carolina, with an AciI and SacI site specific to the rice (Oryjza spp.)-strain and a BsmI and HinfI site joining an already characterized MspI site as diagnostic of the corn (Zea mays L.)-strain. All four of these sites can be detected by digestion of a single 568-bp polymerase chain reaction-amplified fragment, but the use of two enzymes in separate digests was found to provide accurate and rapid determination of strain identity. The effectiveness of this method was demonstrated by the analysis of almost 200 adult and larval specimens from the Mississippi delta region. The results indicated that the corn-strain is likely to be the primary strain infesting cotton (Gossypium spp.) and that an unexpected outbreak of fall armyworm on the ornamental tree Paulownia tomentosa (Thunb.) Sieb. & Zucc. ex Steud. was due almost entirely to the rice-strain.
Nagoshi
Rod N
RN
Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, Gainesville, FL 32608, USA.
Meagher
Robert L
RL
Adamczyk
John J
JJ
Braman
S Kristine
SK
Brandenburg
Rick L
RL
Nuessly
Gregg
G
D000818:Animals; D003576:Electron Transport Complex IV / Q000235:genetics*; D008018:Life Cycle Stages; D008297:Male; D010944:Plants / Q000469:parasitology; D012150:Polymorphism, Restriction Fragment Length; D015144:Southeastern United States; D018411:Spodoptera / Q000235:genetics*
D003576:Electron Transport Complex IV