Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages.
Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.
Hashimoto
Mayu
M
Department of Biosciences and Informatics, Keio University, 3-14-1, Hiyoshi, Yokohama 223-8522, Japan.
Morimoto
Minoru
M
Saimoto
Hiroyuki
H
Shigemasa
Yoshihiro
Y
Yanagie
Hironobu
H
Eriguchi
Masazumi
M
Sato
Toshinori
T
D000818:Animals; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000493:pharmacokinetics*; D004247:DNA; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000138:chemical synthesis; D037181:Lectins, C-Type / Q000378:metabolism / Q000502:physiology; D017737:Macrophages, Peritoneal; D008358:Mannose; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism / Q000502:physiology; D051379:Mice; D018613:Microscopy, Confocal; D010957:Plasmids / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000378:metabolism / Q000502:physiology; D014162:Transfection / Q000379:methods*
D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D004247:DNA; D048271:Chitosan; D008358:Mannose