title: Cellular stress triggers the human topoisomerase I damage response independently of DNA damage in a p53 controlled manner.
abstract: The 'human topoisomerase I (htopoI) damage response' was reported to be triggered by various kinds of DNA lesions. Also, a high and persistent level of htopoI cleavage complexes correlated with apoptosis. In the present study, we demonstrate that DNA damage-independent induction of cell death using colcemid and tumor necrosis factor alpha is also accompanied by a strong htopoI response that correlates with the onset of apoptotic hallmarks. Consequently, these results suggest that htopoI cleavage complex formation may be caused by signaling pathways independent of the kind of cellular stress. Thus, protein interactions or signaling cascades induced by DNA damage or cellular stress might lead to the formation of stabilized cleavage complexes rather than the DNA lesion itself. Finally, we show that p53 not only plays a key role in the regulation of the htopoI response to UV-C irradiation but also to treatment with colcemid.
authors.affiliation: Leibniz Institute for Age Research - Fritz-Lipmann-Institute eV, Biochemistry, Jena, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Rockstroh
authors.forename: A
authors.initials: A
authors.lastname: Kleinert
authors.forename: M
authors.initials: M
authors.lastname: Kramer
authors.forename: F
authors.initials: F
authors.lastname: Grosse
authors.forename: K
authors.initials: K
authors.lastname: Søe
mesh_terms: D015153:Blotting, Western; D004249:DNA Damage; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D018384:Oxidative Stress; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*
chemical_list: D016159:Tumor Suppressor Protein p53; D004264:DNA Topoisomerases, Type I