In vitro effects of some drugs on catalase purified from human skin.
Catalase enzyme (H202: oxidoreductase; E.C. 1.11.1.6) was purified from human skin homogenate using ammonium sulfate precipitation and DEAE-Sephadex A50 ion exchange chromatography at 4 degrees C and some characteristics of the enzyme were investigated. The human skin enzyme, having a specific activity of 1354.5 EU/mg proteins was purified with a yield of 43.13% and 1110-fold. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed a single band for the enzyme. Inhibition by piroxicam, ketoprofen, diclofenac sodium, sulfamethoxazole and nidazole occurred with I50 values of 0.414, 1.29, 1.8, 3.83, and 8.64 mM, respectively.
Altikat
Sayit
S
Dumlupinar University, Nursing Training School, Kutahya, Turkey.
Coban
Abdulkadir
A
Ciftci
Mehmet
M
Ozdemir
Hasan
H
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism / Q000494:pharmacology*; D002374:Catalase / Q000302:isolation & purification / Q000378:metabolism*; D004008:Diclofenac / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D007660:Ketoprofen / Q000494:pharmacology; D010894:Piroxicam / Q000494:pharmacology; D012867:Skin / Q000166:cytology / Q000201:enzymology*; D013420:Sulfamethoxazole / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004791:Enzyme Inhibitors; D010894:Piroxicam; D004008:Diclofenac; D007660:Ketoprofen; D002374:Catalase; D013420:Sulfamethoxazole