Non-surgical alternatives to invasive procedures in mice.
We have developed and validated catheterization protocols in mice that allow for simultaneous infusion and sampling. A sampling catheter was inserted in the lateral vein of the tail, while the animals were infused either intravenously or intragastrically through a second catheter placed in the contralateral lateral vein or via an intragastric catheter, respectively. The applicability of these methods of infusion and blood sampling were validated by conducting urea kinetics utilizing stable isotopes. These non-surgical procedures are non-invasive, inexpensive, fast to perform and animals do not require a recovery period before their use.
Marini
J C
JC
Animal Science Department, University of Illinois, 1207 Gregory Drive, Urbana, IL 61801, USA. jcmarini@uiuc.edu
Lee
B
B
Garlick
P J
PJ
D000818:Animals; D000830:Animals, Laboratory; D001800:Blood Specimen Collection / Q000379:methods / Q000662:veterinary*; D002404:Catheterization / Q000379:methods / Q000662:veterinary*; D008297:Male; D051379:Mice; D009587:Nitrogen Isotopes; D013047:Specific Pathogen-Free Organisms; D014508:Urea / Q000378:metabolism
D009587:Nitrogen Isotopes; D014508:Urea