title: An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice.
abstract: Volatile anesthetics and alcohols enhance transmission mediated by gamma-aminobutyric acid type A receptors (GABA(A)Rs) in the central nervous system, an effect that may underlie some of the behavioral actions of these agents. Substituting a critical serine residue within the GABA(A)R alpha(1) subunit at position 270 with the larger residue histidine eliminated receptor modulation by isoflurane, but it also affected receptor gating (increased GABA sensitivity). To correct the shift in GABA sensitivity of this mutant, we mutated a second residue, leucine at position 277 to alanine. The double mutant alpha(1)(S270H,L277A)beta(2)gamma(2S) GABA(A)R was expressed in Xenopus laevis oocytes and human embryonic kidney (HEK)293 cells, and it had near-normal GABA sensitivity. However, rapid application of a brief GABA pulse to receptors expressed in HEK293 cells revealed that the deactivation was faster in double mutant than in wild-type receptors. In all heterologous systems, the enhancing effect of isoflurane and ethanol was greatly decreased in the double mutant receptor. Homozygous knockin mice harboring the double mutation were viable and presented no overt abnormality, except hyperactivity. This knockin mouse line should be useful in determining which behavioral actions of volatile anesthetics and ethanol are mediated by the GABA(A)Rs containing the alpha(1) subunit.
authors.affiliation: Waggoner Center for Alcohol and Addiction Research, 1 University Station A4800, University of Texas at Austin, Austin, TX 78712-0159, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Borghese
authors.forename: D F
authors.initials: DF
authors.lastname: Werner
authors.forename: N
authors.initials: N
authors.lastname: Topf
authors.forename: N V
authors.initials: NV
authors.lastname: Baron
authors.forename: L A
authors.initials: LA
authors.lastname: Henderson
authors.forename: S L
authors.initials: SL
authors.lastname: Boehm
authors.forename: Y A
authors.initials: YA
authors.lastname: Blednov
authors.forename: A
authors.initials: A
authors.lastname: Saad
authors.forename: S
authors.initials: S
authors.lastname: Dai
authors.forename: R A
authors.initials: RA
authors.lastname: Pearce
authors.forename: R A
authors.initials: RA
authors.lastname: Harris
authors.forename: G E
authors.initials: GE
authors.lastname: Homanics
authors.forename: N L
authors.initials: NL
authors.lastname: Harrison
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D018757:GABA Modulators / Q000494:pharmacology*; D006801:Humans; D007530:Isoflurane / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009043:Motor Activity / Q000187:drug effects; D009154:Mutation; D011963:Receptors, GABA-A / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D013329:Structure-Activity Relationship; D014981:Xenopus; D015032:Zinc / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D018757:GABA Modulators; C546240:GABRA1 protein, human; C546241:Gabra1 protein, mouse; D011963:Receptors, GABA-A; D000431:Ethanol; D005680:gamma-Aminobutyric Acid; D007530:Isoflurane; D015032:Zinc