Multiplex measurement of cytokine/receptor gene polymorphisms and interaction between interleukin-10 (-1082) genotype and chorioamnionitis in extreme preterm delivery.
OBJECTIVE
To establish a multiplex amplification refractory mutation system (ARMS) in fluid and dried whole blood, and to perform a pilot study to examine the role for single-nucleotide polymorphisms (SNPs) of inflammation-associated genes (interleukin [IL]-1 and -10, tumor necrosis factor-alpha [TNFA], and toll-like receptor-4 [TLR4]) and their interaction with clinical chorioamnionitis (CAM) in prematurity.


METHODS
We established a quadruplex ARMS to detect the four above SNPs. Fifty-four women delivered at gestational age less than 32 weeks and 83 healthy female volunteers were genotyped. We compared (1) mothers of preterm infants with volunteers, and (2) women delivered before 29 weeks' gestation (n = 29) with those delivered at 29 to 31 completed weeks (n = 25).


RESULTS
Multiplex ARMS is feasible using both fluid and dried whole blood. We found no overall differences in genotype and allele frequencies between mothers of preterm infants and volunteers. Among women who had a preterm delivery, those with both CAM and IL10(-1082)*G allele, the risk for delivery before 29 weeks was markedly increased (odds ratio [OR] 22, 95% confidence interval [CI] 2.5 - 191).


CONCLUSIONS
The presence of both CAM and IL10(-1082)*G might play a role in extreme preterm delivery less than 29 weeks.
Kerk
Julia
J
Department of Gynecology and Obstetrics, Hannover Medical School, Hannover, Germany.
Dördelmann
Michael
M
Bartels
Dorothee B
DB
Brinkhaus
Maria-Jantje
MJ
Dammann
Christiane E L
CE
Dörk
Thilo
T
Dammann
Olaf
O
D002821:Chorioamnionitis / Q000235:genetics*; D016207:Cytokines / Q000235:genetics*; D004247:DNA / Q000097:blood; D005260:Female; D005838:Genotype; D005865:Gestational Age; D017359:HELLP Syndrome / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D007375:Interleukin-1 / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics*; D007752:Obstetric Labor, Premature / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011272:Pregnancy, Multiple; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016207:Cytokines; D007375:Interleukin-1; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D004247:DNA