Novel design of multicapillary arrays for high-throughput DNA sequencing.
A novel approach to design and optimize linear multicapillary arrays (LMCAs) for high-throughput DNA sequencing is proposed. A significant increase in the number of capillary lanes is obtained due to the use of composite insertions alternately placed between working capillaries of the array and a specific combination of refractive indices of the DNA separation matrix, capillary glass, the insertions and a medium which surrounds the capillary array. Theoretical and experimental studies showed that in conjunction with a dual-side laser illumination scheme, the proposed LMCA design allows a simultaneous uniform irradiation of as many as 550 working capillaries.
Tsupryk
Andriy
A
Electrical and Computer Engineering Department, State University of New York at Stony Brook, Stony Brook, NY, USA. atsupryk@ic.sunysb.edu
Gorbovitski
Michael
M
Kabotyanski
Evgeni A
EA
Gorfinkel
Vera
V
D003198:Computer Simulation; D019075:Electrophoresis, Capillary; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D017422:Sequence Analysis, DNA / Q000379:methods*