High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma.
MSP001 is a newly synthesized piperidine analog of the lead antihypertensive compound MS23 that dually targets cAMP-specific type 4 phosphodiesterase and L-type calcium channels. We validated an analytical protocol for MSP001 in rat plasma using high performance liquid chromatographic method. A C18 column and a phosphate/acetonitrile buffer were used to perform the chromatographic separation. UV detection was carried out at 307 nm, a wavelength at which an absorption peak was detected for this group of compounds. The calibration curve for MSP001 was linear in the range from 25 to 10,000 ng/ml. The limit of quantification (LOQ) was 25 ng/ml. The results demonstrate that this method has high linearity (R=0.99995), compound specificity, and acceptable precision/accuracy. The protocol is suitable for in vivo pharmacokinetic studies on the compound.
Wang
Ting
T
Section of Cardiology, Department of Medicine, Pritzker School of Medicine, University of Chicago, 5841 S. Maryland Ave., MC6088, Chicago, IL 60637, USA.
Wang
Desuo
D
D000818:Animals; D000959:Antihypertensive Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D005260:Female; D007275:Injections, Intravenous; D010880:Piperidines / Q000097:blood*; D011758:Pyrroles / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000959:Antihypertensive Agents; C508412:MS23 agent; C516728:MSP001; D010880:Piperidines; D011758:Pyrroles