title: [Expression and purification of human MT-2a fusion protein in prokaryotic cells and preparation of its antiserum].
abstract: OBJECTIVE
  To express and purify human MT-2a in prokaryotic cells and to prepare the MT-2a-specific rabbit antiserum.
  
  
  METHODS
  GST-MT-2a fusion protein was expressed after IPTG induction and further purified with Glutathione Sepharose 4B. Then the purified GST-MT-2a fusion protein was used to immunize New Zealand rabbits. The titer and specificity of rabbit antiserum were evaluated by double immunodiffusion, ELISA and Western blot.
  
  
  RESULTS
  GST-MT-2a fusion protein was highly expressed. The final yield of the pure GST-MT-2a was about 38 mg per liter of bacterial culture. Its antiserum with high specificity and potency was also obtained.
  
  
  CONCLUSIONS
  The successful expression of GST-MT-2a fusion protein in E. coli and the preparation of MT-2a specific rabbit antiserum will be valuable for the study on the function of human MT-2a.
authors.affiliation: Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China. hezhimin2005@yahoo.com
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: He
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Liu
authors.forename: Zhu-chu
authors.initials: ZC
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000918:Antibody Specificity; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D008668:Metallothionein / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D011387:Prokaryotic Cells / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D012995:Solubility
chemical_list: D007106:Immune Sera; C491053:MT2A protein, human; D011993:Recombinant Fusion Proteins; D008668:Metallothionein