Amplification of CCND1, EMS1, PIK3CA, and ERBB oncogenes in ethmoid sinus adenocarcinomas.
OBJECTIVE
Assess the amplification of CCND1, EMS1, PIK3CA, ERBB1, and ERBB2 oncogenes in ethmoid sinus adenocarcinomas.


METHODS
Tissue samples from 13 primary ethmoid adenocarcinomas and 2 recurrences were studied at Hospital Central de Asturias, Oviedo, Spain, between July 1998 and February 2002. A semiquantitative evaluation of CCND1, EMS1, PIK3CA, ERBB1, and ERBB2 amplification was performed by multiplex polymerase chain reaction.


RESULTS
Three (23%) cases presented CCND1 amplification, one of them with a concurrent PIK3CA amplification. EMS1 was amplified in the 2 studied recurrences. ERBB1 was amplified in 1 case (8%), whereas none presented ERBB2 amplification. Oncogenic amplifications were only detected in advanced (stage III) tumors; however, no prognostic value could be shown for them.


CONCLUSIONS
CCND1, EMS1, PIK3CA, and ERBB1 amplifications are uncommon and appear to be late events in the development of ethmoid sinus adenocarcinoma.


CONCLUSIONS
New information on the carcinogenesis of this infrequent sinonasal tumor is presented.
Nazar
Gonzalo
G
Department of Otolaryngology, Hospital Central de Asturias, Oviedo, Spain. gnazar@clinicalascondes.cl
González
María V
MV
García
Juana María
JM
Llorente
José L
JL
Rodrigo
Juan P
JP
Suárez
Carlos
C
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D058534:Class I Phosphatidylinositol 3-Kinases; D051356:Cortactin / Q000235:genetics*; D019938:Cyclin D1 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005005:Ethmoid Sinus; D015972:Gene Expression Regulation, Neoplastic; D018773:Genes, erbB-1 / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D010255:Paranasal Sinus Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*; D016133:Polymerase Chain Reaction
D014408:Biomarkers, Tumor; C495868:CTTN protein, human; D051356:Cortactin; D004273:DNA, Neoplasm; D019938:Cyclin D1; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human