Human laminin-5 and laminin-10 mediated gene expression of prostate carcinoma cells.
In prostate cancer progression, the basal lamina switches from predominantly laminin-5 to laminin-10. DU-145 prostate cancer cells were treated with either soluble laminin-5 (20 ng/ml) or laminin-10 (1 microg/ml) for 6, 24, and 48 hr. Total RNA was harvested for a 7,500 human cDNA microarray. Hybridizations were carried out in accordance with a 10 sample analysis of variance (ANOVA) statistical model. One thousand one hundred sixteen genes had measurable expression 2 standard deviations above background and 50% of spots for any given sample for all hybridizations were positive. Expression values of significantly varying genes were clustered and a list of 408 genes (P < 0.05) with a 1.5 or greater fold change in at least one time point were chosen for further analysis. Seventy eight changed in a time-dependent manner with laminin-10 treatment, 85 changed with laminin-5, and 13 showed changes with both treatments. The 408 genes that passed a paired t-test in at least one time-dependent category were further analyzed using Pathway Miner. One of the largest gene association networks involved signal transduction in the growth factor-MAP kinase pathways. EGFR was validated by real-time PCR and laminin-10 mediated cell adhesion activated EGFR in DU-145 cells. Both laminins appear to be important signal transducers in prostate cancer.
Calaluce
Robert
R
Arizona Cancer Center, University of Arizona Health Sciences Center, Tucson, Arizona 85724-5024, USA.
Beck
Shaleen K
SK
Bair
Elisabeth L
EL
Pandey
Ritu
R
Greer
Kevin A
KA
Hoying
Adam M
AM
Hoying
James B
JB
Mount
David W
DW
Nagle
Raymond B
RB
D002154:Calpain / Q000235:genetics / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000502:physiology*; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007797:Laminin / Q000502:physiology*; D020935:MAP Kinase Signaling System / Q000235:genetics; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012334:RNA, Neoplasm / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D013997:Time Factors; D000097794:Kalinin
D002154:Calpain; D015815:Cell Adhesion Molecules; D004273:DNA, Neoplasm; D066246:ErbB Receptors; D007797:Laminin; D012334:RNA, Neoplasm; D000097794:Kalinin; C119013:laminin 10