title: Stabilization of a plasmid coding for a heterologous antigen in Salmonella enterica serotype typhi vaccine strain CVD908-htrA by using site-specific recombination.
abstract: A gene cassette incorporating the crs-rsd site-specific recombination system from the Salmonella enterica subsp. enterica serovar Dublin virulence plasmid improved the inheritance in S. enterica serotype Typhi strain CVD908-htrA of a multicopy plasmid expression vector. Use of this recombination cassette may improve expression of heterologous antigens from multicopy plasmid expression vectors in attenuated bacterial vaccine strains.
authors.affiliation: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom.
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Stephens
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Darsley
authors.forename: Arthur K
authors.initials: AK
authors.lastname: Turner
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D015478:Antigens, Heterophile / Q000235:genetics* / Q000276:immunology; D005822:Genetic Vectors / Q000378:metabolism; D016480:Helicobacter pylori / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000378:metabolism*; D011995:Recombination, Genetic; D022562:Salmonella Vaccines / Q000235:genetics* / Q000276:immunology; D012485:Salmonella typhi / Q000235:genetics* / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D015478:Antigens, Heterophile; D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated