title: Application of microdialysis to study the in vivo release of pingyangmycin from in situ gels in rabbits.
abstract: Microdialysis was used together with HPLC to monitor the local concentration of pingyangmycin hydrochloride (PYM) after embolizing rabbit ear-veins with an injectable sustained release formulation, PYM-Zein/PYM-Zein-sucrose acetate isobutyrate (SAIB), in situ gel. The dialysis probe was perfused at 2 microL/min. The in vivo recovery was 46.6 3.1% (n = 4). The samples were injected directly into HPLC. PYM was detected using UV detector at 291 nm. Separation from other components in the dialysate was performed using a Discovery((R)) RP-Amide C(16) column within 12 min. The response for PYM in the dialysate was linear (r(2) > 0.996) over the range of 2.5-212 microg/mL. The limit of detection and limit of quantitation of PYM in the dialysate were 0.4 and 1.5 microg/mL, respectively. The results demonstrated that the in situ gel of PYM-Zein-SAIB could extend the release of PYM to 4 days. SAIB could significantly cut down the initial burst of PYM from the in situ gels (p < 0.05).
authors.affiliation: Department of Pharmaceutics, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, People's Republic of China.
authors.forename: Zi-bin
authors.initials: ZB
authors.lastname: Gao
authors.forename: Ping-tian
authors.initials: PT
authors.lastname: Ding
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhen-ting
authors.initials: ZT
authors.lastname: Yuan
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.forename: Da-wei
authors.initials: DW
authors.lastname: Chen
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D017551:Microdialysis / Q000379:methods*; D011817:Rabbits; D015203:Reproducibility of Results
chemical_list: D001761:Bleomycin; C025703:bleomycetin