title: Genomic instability of human aberrant crypt foci measured by inter-(simple sequence repeat) PCR and array-CGH.
abstract: Aberrant crypt foci (ACF) are the earliest identifiable neoplastic lesions in the colon. Thirty-two ACFs were examined for genomic instability in forms detectable either by inter-(simple sequence repeat) PCR or by array comparative genomic hybridization [array-CGH]. One-fourth of ACFs revealed moderate instability by inter-(simple sequence repeat) PCR; none showed amplifications or deletions on array-CGH. The absence of genomic events detectible by BAC array-CGH indicates early events in colorectal tumor progression are typically smaller than the approximate 150 kb size of a BAC clone insert.
authors.affiliation: Division of Gastrointestinal Surgery, Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors.forename: Sadir J
authors.initials: SJ
authors.lastname: Alrawi
authors.forename: Robert E
authors.initials: RE
authors.lastname: Carroll
authors.forename: Hank C
authors.initials: HC
authors.lastname: Hill
authors.forename: John F
authors.initials: JF
authors.lastname: Gibbs
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Tan
authors.forename: Bruce M
authors.initials: BM
authors.lastname: Brenner
authors.forename: Norma J
authors.initials: NJ
authors.lastname: Nowak
authors.forename: Helen
authors.initials: H
authors.lastname: Swede
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Stoler
authors.forename: Garth R
authors.initials: GR
authors.lastname: Anderson
mesh_terms: D000369:Aged, 80 and over; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology*; D005260:Female; D015894:Genome, Human / Q000235:genetics; D042822:Genomic Instability / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology*