Using gelatin scaffold with coated basic fibroblast growth factor as a transfer system for transplantation of human neural stem cells.
Gelatin scaffolds for ex vivo cell cultures are a promising development. These scaffolds can be used as three-dimensional skeletons for cell attachment and culture before transplantation. In this study, we isolated and cultivated neural stem cells from human brain tissues in serum-free medium (DMEM+F12 nutrient). Better neuron growth was observed using the tetrazolium assay (MTT) in the group when basic fibroblast growth factor (bFGF) was coated on the gelatin polymer scaffold. Further development of this nontoxic system may help the future development of transplantation of human neural stem cells.
Chen
Y-W
YW
Cancer Center, Department of Medical Research & Education, Taipei Veterans General Hospital, and Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
Chiou
S-H
SH
Wong
T-T
TT
Ku
H-H
HH
Lin
H-T
HT
Chung
C-F
CF
Yen
S-H
SH
Kao
C-L
CL
D017690:Cell Transplantation / Q000379:methods; D004827:Epilepsy / Q000628:therapy; D005346:Fibroblast Growth Factors / Q000502:physiology*; D005780:Gelatin; D006801:Humans; D009420:Nervous System / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*
D005346:Fibroblast Growth Factors; D005780:Gelatin