Production of high-content galacto-oligosaccharide by enzyme catalysis and fermentation with Kluyveromyces marxianus.
Of three beta-galactosidases from Aspergillus oryzae, Kluyveromyces lactis and Bacillus sp., used for the production of low-content galacto- oligosaccharides (GOS) from lactose, the latter produced the highest yield of trisaccharides and tetrasaccharides. GOS production was enhanced by mixing beta-galactosidase glucose oxidase. The low-content GOS syrups, produced either by beta-galactosidase alone or by the mixed enzyme system, were subjected to the fermentation by Kluyveromyces marxianus, whereby glucose, galactose, lactose and other disaccharides were depleted, resulting in up to 97% and 98% on a dry weight basis of high-content GOS with the yields of 31% and 32%, respectively.
Cheng
Chao-Chun
CC
Department of Bioengineering, Tatung University, Taipei 104, Taiwan.
Yu
Mei-Ching
MC
Cheng
Tzu-Chien
TC
Sheu
Dey-Chyi
DC
Duan
Kow-Jen
KJ
Tai
Wei-Lun
WL
D019149:Bioreactors; D002384:Catalysis; D004187:Disaccharides / Q000378:metabolism; D005285:Fermentation / Q000502:physiology*; D005690:Galactose / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D007716:Kluyveromyces / Q000201:enzymology*; D009844:Oligosaccharides / Q000096:biosynthesis*; D019936:Probiotics; D001616:beta-Galactosidase / Q000378:metabolism
D004187:Disaccharides; D009844:Oligosaccharides; D005949:Glucose Oxidase; D001616:beta-Galactosidase; D005690:Galactose