title: Antimicrobial properties of the chelating agent EDTA on Streptococcal bovine mastitis isolates.
abstract: To determine the efficacy of the chelating agent EDTA on microbial growth, separate cultures of two streptococcal bovine mastitis isolates, Streptococcus agalactiae and Streptococcus uberis, were exposed to known concentrations of EDTA. Bacterial cultures of 10(8) CFU/ml were exposed to concentrations of EDTA ranging from 30 to 100 mM in an in-vitro-milk environment. Multiple replications of cultures exposed to EDTA were plated during a two-hour time course. A concentration of 100 mM EDTA resulted in a 90% reduction of S. agalactiae and a 99% reduction of S. uberis. Under these experimental conditions, EDTA treatments in cultures of both isolates exhibited from 1 to 2 log reductions suggesting that EDTA is a potentially effective antimicrobial against streptococcal isolates implicated in causing bovine mastitis.
authors.affiliation: Dairy Food Safety Laboratory, Veterinary Medicine and Teaching Research Center, School of Veterinary Medicine, University of California, Davis, California 95616, USA.
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Reidmiller
authors.forename: Wayne L
authors.initials: WL
authors.lastname: Smith
authors.forename: Mary M
authors.initials: MM
authors.lastname: Sawyer
authors.forename: Bennie I
authors.initials: BI
authors.lastname: Osburn
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Stott
authors.forename: James S
authors.initials: JS
authors.lastname: Cullor
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D002614:Chelating Agents; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology*; D005260:Female; D005503:Food Additives / Q000494:pharmacology*; D005506:Food Contamination; D005516:Food Microbiology; D008414:Mastitis, Bovine / Q000188:drug therapy; D008892:Milk / Q000382:microbiology*; D013291:Streptococcus / Q000187:drug effects*; D013292:Streptococcus agalactiae / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D002614:Chelating Agents; D005503:Food Additives; D004492:Edetic Acid