Extensive analysis of different allelelic structures of the chicken BF2 and beta2m proteins.
No information is available to date on the different allelelic structures of the chicken MHC class I (BF2) and beta2m proteins. To elucidate the structure, new allelic beta2m and five different BF2 genes were expressed solubly and purified in a pMAL-p2X/E. coli TB1 system. The 2D structure was detected by circular dichroism (CD) spectroscopy, and the 3D structures of their peptide-binding domain (PBD) were analyzed by homology modeling. The sequence lengths of the alpha-helix, beta-sheet, turn, and random coil in the five BF2 proteins were 69-73 aa, 67-72 aa, 35-37 aa, and 94-98 aa, respectively. The new beta2m protein displayed a typical beta-sheet. Homology modeling of the different BF2 and beta2m proteins demonstrated similarities to the structure of human and rat MHC class I proteins. The 3D structure, however, revealed that the BF2 and beta2m structures were unique. The correct refolding of recombinant BF2 and beta2m proteins might be a powerful tool to further detect antigenic peptides.
Li
Xin Sheng
XS
Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, P.R. China.
Fang
Qin Mei
QM
Yan
Ruo Qian
RQ
Gao
Feng Shan
FS
Hao
Hui Fang
HF
Jia
Zhen Hu
ZH
Lin
Chang You
CY
Xia
Chun
C
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D002942:Circular Dichroism / Q000662:veterinary; D004247:DNA / Q000737:chemistry / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D017422:Sequence Analysis, DNA; D001613:beta 2-Microglobulin / Q000235:genetics* / Q000276:immunology
D015395:Histocompatibility Antigens Class I; D011994:Recombinant Proteins; D001613:beta 2-Microglobulin; D004247:DNA