title: Increased cell-to-cell variation in gene expression in ageing mouse heart.
abstract: The accumulation of somatic DNA damage has been implicated as a cause of ageing in metazoa. One possible mechanism by which increased DNA damage could lead to cellular degeneration and death is by stochastic deregulation of gene expression. Here we directly test for increased transcriptional noise in aged tissue by dissociating single cardiomyocytes from fresh heart samples of both young and old mice, followed by global mRNA amplification and quantification of mRNA levels in a panel of housekeeping and heart-specific genes. Although gene expression levels already varied among cardiomyocytes from young heart, this heterogeneity was significantly elevated at old age. We had demonstrated previously an increased load of genome rearrangements and other mutations in the heart of aged mice. To confirm that increased stochasticity of gene expression could be a result of increased genome damage, we treated mouse embryonic fibroblasts in culture with hydrogen peroxide. Such treatment resulted in a significant increase in cell-to-cell variation in gene expression, which was found to parallel the induction and persistence of genome rearrangement mutations at a lacZ reporter locus. These results underscore the stochastic nature of the ageing process, and could provide a mechanism for age-related cellular degeneration and death in tissues of multicellular organisms.
authors.affiliation: Buck Institute for Age Research, Novato, California 94945, USA.
authors.forename: Rumana
authors.initials: R
authors.lastname: Bahar
authors.forename: Claudia H
authors.initials: CH
authors.lastname: Hartmann
authors.forename: Karl A
authors.initials: KA
authors.lastname: Rodriguez
authors.forename: Ashley D
authors.initials: AD
authors.lastname: Denny
authors.forename: Rita A
authors.initials: RA
authors.lastname: Busuttil
authors.forename: Martijn E T
authors.initials: ME
authors.lastname: Dollé
authors.forename: R Brent
authors.initials: RB
authors.lastname: Calder
authors.forename: Gary B
authors.initials: GB
authors.lastname: Chisholm
authors.forename: Brad H
authors.initials: BH
authors.lastname: Pollock
authors.forename: Christoph A
authors.initials: CA
authors.lastname: Klein
authors.forename: Jan
authors.initials: J
authors.lastname: Vijg
mesh_terms: D000375:Aging / Q000235:genetics* / Q000502:physiology; D000818:Animals; D015870:Gene Expression; D006321:Heart / Q000502:physiology; D006861:Hydrogen Peroxide; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D006861:Hydrogen Peroxide