Immobilization of proteins on agarose beads, monitored in real time by bead injection spectroscopy.
A novel approach to real-time monitoring of protein immobilization resulted in the surprising finding that current immobilization protocols are far from optimized.
Ruzicka
Jaromir
J
Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA.
Carroll
Andrea D
AD
Lähdesmäki
Ilkka
I
D017022:Flow Injection Analysis / Q000379:methods; D008832:Microchemistry / Q000379:methods*; D008863:Microspheres; D011506:Proteins / Q000737:chemistry*; D012685:Sepharose; D013057:Spectrum Analysis / Q000379:methods
D011506:Proteins; D012685:Sepharose