[Research of modulation of CD95-mediated apoptosis in lymphoblastic MP-1 and BJAB cells infected by adenovirus and Epstein-Barr virus].
Model systems of infecting limphoblastic MP-1 and BJAB cells by Epstein-Barr virus, 5 serotype adenovirus and double infection are developed. A rather high level of accumulation of DNA of these viruses in the cells in dynamics at monoinfection and inhibition interference at multi-infection was shown by PCR method. The influence of virus infection on proliferative activity was studied. The stimulation of cells growth in the system BJAB + EBV was detected, and double infecting inhibited the process by 50%. The 25% difference in development of apoptosis process between cells infected by adenovirus and EBV was established when defining CD95-mediated apoptosis in infected MP-1 cells. The infecting of BJAB cells by viruses had a scarce effect on the processes of spontaneous apoptosis, but the data on CD95-mediated apoptosis at EBV infection testify to inhibition of this process both at a monoinfection, and at a double infection. The work was performed in the framework of the fundamental agreement of Ministry of Education and Science of Ukraine F7/366-2001, and grant INTAS N011-2382.
Nesterova
N V
NV
Diachenko
N S
NS
Zahorodnia
S D
SD
Nosach
L M
LM
Povnytsia
O Iu
OIu
Baranova
H V
HV
Zhovnovata
V L
VL
D000256:Adenoviridae / Q000235:genetics / Q000254:growth & development*; D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism / Q000473:pathology / Q000821:virology; D019014:fas Receptor / Q000378:metabolism*
D004279:DNA, Viral; D019014:fas Receptor