Enhanced culture of Borrelia garinii and Borrelia afzelii strains on a solid BSK-based medium in anaerobic conditions.
The growth of 29 human strains from the three main pathogenic species of Borrelia burgdorferi sensu lato on a solid BSK-based medium was compared in two culture atmospheres: 3% CO(2) air and anaerobiosis. All strains grew under anaerobic conditions, whereas only 13 strains were able to grow in aerobiosis with 3% CO(2) (P<0.001). In the latter condition, 75% of the B. burgdorferi sensu stricto strains grew versus 33% of the B. garinii and B. afzelii strains. These data suggest that, especially for B. garinii and B. afzelii species, anaerobic conditions enhance growth yield and speed of low-passage Borrelia strains.
De Martino
Sylvie Josiane
SJ
Laboratoire de Physiopathologie et d'Antibiologie Microbiennes, EA 3432, Laboratoire de Bactériologie de la Faculté de Médecine et Hopitaux Universitaires de Strasbourg, 3 rue Koeberlé, 67000 Strasbourg, France.
Sordet
Christelle
C
Piémont
Yves
Y
Ruzic-Sabljic
Eva
E
Thaddée Vetter
Marie
M
Monteil
Henri
H
Sibilia
Jean
J
Jaulhac
Benoît
B
D000693:Anaerobiosis; D001431:Bacteriological Techniques; D015748:Borrelia burgdorferi Group / Q000254:growth & development*; D018929:Cell Culture Techniques; D003470:Culture Media; D050296:Microbial Viability
D003470:Culture Media