title: Regulation of poly(A) binding protein function in translation: Characterization of the Paip2 homolog, Paip2B.
abstract: The 5' cap and 3' poly(A) tail of eukaryotic mRNAs act synergistically to enhance translation. This synergy is mediated via interactions between eIF4G (a component of the eIF4F cap binding complex) and poly(A) binding protein (PABP). Paip2 (PABP-interacting protein 2) binds PABP and inhibits translation both in vitro and in vivo by decreasing the affinity of PABP for polyadenylated RNA. Here, we describe the functional characteristics of Paip2B, a Paip2 homolog. A full-length brain cDNA of Paip2B encodes a protein that shares 59% identity and 80% similarity with Paip2 (Paip2A), with the highest conservation in the two PABP binding domains. Paip2B acts in a manner similar to Paip2A to inhibit translation of capped and polyadenylated mRNAs both in vitro and in vivo by displacing PABP from the poly(A) tail. Also, similar to Paip2A, Paip2B does not affect the translation mediated by the internal ribosome entry site (IRES) of hepatitis C virus (HCV). However, Paip2A and Paip2B differ with respect to both mRNA and protein distribution in different tissues and cell lines. Paip2A is more highly ubiquitinated than is Paip2B and is degraded more rapidly by the proteasome. Paip2 protein degradation may constitute a primary mechanism by which cells regulate PABP activity in translation.
authors.affiliation: Department of Biochemistry and McGill Cancer Center, McGill University, Montréal, Québec, Canada. jberlanga@cbm.uam.es
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Berlanga
authors.forename: Alexis
authors.initials: A
authors.lastname: Baass
authors.forename: Nahum
authors.initials: N
authors.lastname: Sonenberg
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002474:Cell-Free System; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D039603:Eukaryotic Initiation Factor-4G / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D010448:Peptide Initiation Factors / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D039103:Poly(A)-Binding Protein II / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D039603:Eukaryotic Initiation Factor-4G; D010448:Peptide Initiation Factors; D039103:Poly(A)-Binding Protein II; D012315:RNA Caps; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D025801:Ubiquitin; D005982:Glutathione Transferase; D046988:Proteasome Endopeptidase Complex