[The effect of duplications in the T-DNA on the stability of manifestation of heterologous genes in transgenic tobacco plants].
The duplication of uidA gene within T-DNA was shown to disturb stability of expression of another marker gene, nptII, in the second generation (T2) of selfed initial transformants and in F1 hybrids of the crosses with nontransgenic tobacco. Hybridological analysis of the progeny resulting from various crosses involving T1 plants demonstrated that the expression of nptII gene was impaired in the hybrids that were hemizygous for the inactivated copy of uidA gene.
Marenkova
T V
TV
Deĭneko
E V
EV
D004269:DNA, Bacterial / Q000235:genetics*; D018744:DNA, Plant; D020440:Gene Duplication; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005819:Genetic Markers; D030821:Plants, Genetically Modified / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
D004269:DNA, Bacterial; D018744:DNA, Plant; D005819:Genetic Markers; C036483:T-DNA