Competitive reactions of L-methionine and 5'-GMP towards platinum (II) complexes.
The complex-formation reactions of the platinum(II) complexes, [Pt(dien)H2O]2+, [PtCl(dien)]+ and [PtBr(dien)]+ (dien is diethylenetriamine) with some biologically relevant ligands such as inosine (INO), inosine-5'-monophosphate (5'-IMP), guanosine-5'-monophosphate (5'-GMP), glutathione (GSH) and L-methionine (S-meth), were studied by UV-Vis (UV-Visible) spectrophotometry and 1H NMR spectroscopy. Reactions of the [PtCl(dien)]+ with L-methionine were studied in the presence and absence of 5'-GMP. The rate constants clearly showed a kinetic preference toward L-methionine. However, competitive reactions of [PtCl(dien)]+ with L-methionine and 5'-GMP demonstrated initially rapid formation of [Pt(dien)(S-meth)]2+ followed by displacement of L-methionine by 5'-GMP. In the later stages the concentration of [Pt(dien)(N7-GMP )]2+ is predominant. The results are analyzed in reference to the anti-tumour activity of Pt(II) complexes.
Soldatović
Tanja
T
University of Kragujevac, Faculty of Science, Department of Chemistry; R. Domanovic'a 12, P. O. Box 60, 34000 Kragujevac, Serbia.
Canović
Predrag
P
Canović
Dragan
D
Bugarcić
Zivadin D
ZD
D001667:Binding, Competitive; D005978:Glutathione; D006157:Guanosine Monophosphate / Q000737:chemistry*; D007288:Inosine / Q000737:chemistry; D007291:Inosine Monophosphate / Q000737:chemistry; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008715:Methionine / Q000737:chemistry*; D015394:Molecular Structure; D009944:Organoplatinum Compounds / Q000737:chemistry; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013329:Structure-Activity Relationship
D008024:Ligands; D009944:Organoplatinum Compounds; D007291:Inosine Monophosphate; D007288:Inosine; D006157:Guanosine Monophosphate; D008715:Methionine; D005978:Glutathione