Dissection of additive, epistatic effect and Q x E interaction of quantitative trait loci influencing stigma exsertion under water stress in rice.
Four flowering related traits, spikelet number per panicle (SNP), percentage of single exserted stigma (PSES), dual exserted stigma (PDES) and total exserted stigma (PES) of a RI population with 185 lines under water stress and non-stress conditions for 2 years, were investigated in a drought tolerance screening facility. ANOVA results showed high significance between years, lines, and water stress treatments, together with interactions among them in pairs. Highest phenotypic correlation was found between PSES and PES (r = 0.9752***), followed by PDES and PES (r = 0.7150***), and PSES and PDES (r = 0.5424***). Based on a linkage map of 203 SSR markers, six main effect QTLs were detected for SNP and three or four main effect QTLs were associated with PSES, PDES and PES under stress or non-stress conditions. There were one to nine pairs of epistatic QTLs influencing SNP and stigma exsertion. The contribution rates of additive and epistatic effects seemed to be in a low magnitude for most cases (0.76%-9.92%) while a few QTLs or QTL pairs explained more than 10% of total variance. Some main effect QTL and epistasis were commonly detected among PSES, PDES and PES, explaining the high positive correlation between them. Few QTLs were detected under both water stress and non-stress condition, implying that drought had severe impact on the genetic behaviors of both spikelet number and stigma exsertion.
Yu
Xin-Qiao
XQ
Shanghai Agrobiological Gene Center, China.
Mei
Han-Wei
HW
Luo
Li-Jun
LJ
Liu
Guo-Lan
GL
Liu
Hong-Yan
HY
Zou
Gui-Hua
GH
Hu
Song-Ping
SP
Li
Ming-Sou
MS
Wu
Jin-Hong
JH
D018556:Crops, Agricultural / Q000235:genetics; D003681:Dehydration / Q000235:genetics*; D004843:Epistasis, Genetic; D035264:Flowers / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D040641:Quantitative Trait Loci; D012639:Seeds