Improvement in yield and purity of a recombinant malaria vaccine candidate based on the receptor-binding domain of Plasmodium vivax Duffy binding protein by codon optimization.
A recombinant blood-stage vaccine for Plasmodium vivax malaria based on the functional receptor-binding domain of PvDBP (PvRII) has been developed. A synthetic gene coding for PvRII was expressed in Escherichia coli using codon optimization. Expression level of recombinant PvRII was 10% of the total cellular proteins. Truncated PvRII products, seen when the native PvRII gene was expressed, were absent in case of synthetic gene.
Yazdani
Syed Shams
SS
Malaria Research Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, India. shams@icgeb.res.in
Shakri
Ahmad Rushdi
AR
Pattnaik
Priyabrata
P
Rizvi
M Moshahid A
MM
Chitnis
Chetan E
CE
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D024861:Genetic Enhancement / Q000379:methods*; D017780:Malaria Vaccines / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D010966:Plasmodium vivax / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods
D000953:Antigens, Protozoan; D003062:Codon; C066001:Duffy antigen binding protein, Plasmodium; D017780:Malaria Vaccines; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins