Over-production of beta-carotene from metabolically engineered Escherichia coli.
To produce recombinant beta-carotene in vitro, synthetic operons encoding genes governing its biosynthesis were engineered into Escherichia coli. Constructs harboring these operons were introduced into either a high-copy or low-copy cloning vector. beta-Carotene production from these recombinant E. coli cells was either constitutive or inducible depending upon plasmid copy number. The most efficient beta-carotene production was with the low-copy based vector. The process was increased incrementally from a 5 l to a 50 l fermentor and finally into a 300 l fermentor. The maximal beta-carotene yields achieved using the 50 l and 300 l fermentor were 390 mg l(-1) and 240 mg l(-1), respectively, with overall productivities of 7.8 mg l(-1) h(-1) and 4.8 mg l(-1) h(-1).
Kim
Sung-Woo
SW
AceBiotech Co Ltd, No 114 Bio-Venture Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Kim
Jae-Bum
JB
Jung
W-H
WH
Kim
Jung-Hoe
JH
Jung
Joon-Ki
JK
D004345:Drug Industry / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D009876:Operon / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism*; D019207:beta Carotene / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D019207:beta Carotene