Differential expression profiling of genes in a complete hydatidiform mole using cDNA microarray analysis.
OBJECTIVE
To gain a better understanding of the genes involved in the pathogenesis of gestational trophoblastic diseases, we evaluated the genome-wide expression levels of genes in complete hydatidiform mole (H-mole) as compared to normal placenta using cDNA microarray technique.


METHODS
The expression profiles of complete H-mole tissues were compared with those of normal placenta using cDNA microarray technique. The data obtained from 10,305 human genes were normalized by the print-tip-based LOWESS method. Significance analysis of microarray (SAM) was used to identify genes with statistically significant changes in expression. The expression levels of genes which showed significant differences between normal early placenta and complete H-mole tissues were further confirmed by RT-PCR.


RESULTS
A cDNA microarray analysis consisting of 10,305 human genes revealed significant changes in the expression of 213 genes, with 91 genes being upregulated and 122 being downregulated. SAM revealed significant changes in gene expression, including those associated with signal transduction, cell structure, transcription, and apoptosis. Further RT-PCR analysis of altered gene expression in mole tissues supported the microarray analysis results. We confirmed the upregulation of TLE4, CAPZA1, PRSS25, RNF130, and USP1 in complete H-mole tissues. Moreover, our study provides the first evidence that ELK3, LAMA3, LNK, STAT2, and TNFRSF25 are downregulated in complete H-mole compared to normal early placenta tissues.


CONCLUSIONS
These findings provide a large body of information regarding gene expression profiles associated with complete H-mole tumorigenesis and allow the identification of potential targets for tumor prevention or therapy.
Kim
Seung Jo
SJ
Comprehensive Gynecologic Cancer Center, CHA General Hospital, Kyonggi-Do, South Korea.
Lee
Sun Young
SY
Lee
Chan
C
Kim
Inho
I
An
Hee Jung
HJ
Kim
Ji Young
JY
Baek
Kwang Hyun
KH
Kim
Eun Joong
EJ
Kim
Jung Mogg
JM
Lee
Jung Bok
JB
Lee
Jae Won
JW
Jung
Woon-Won
WW
Chun
Taehoon
T
Oh
Yu-Kyoung
YK
D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D015894:Genome, Human; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000378:metabolism / Q000502:physiology; D011247:Pregnancy
D018076:DNA, Complementary