Synthesis and evaluation of in vitro antioxidant capacities of some benzimidazole derivatives.
New, except 1d, melatonin analogue benzimidazole derivatives were synthesized and characterized in the present study. The potential role of melatonin as an antioxidant by scavenging and detoxifying ROS raised the possibility that compounds that are analogous to melatonin can also be used for their antioxidant properties. Therefore the antioxidant effects of the newly synthesized compounds were investigated in vitro by means of their inhibitory effect on hydrogen peroxide-induced erythrocyte membrane lipid peroxidation (EMLP) and on various erythrocyte antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and glucose-6-phosphate dehydrogenase (G6PD). The synthesized benzimidazole derivatives showed remarkable antioxidant activity in vitro in the H2O2-induced EMLP system. Furthermore their effects on various antioxidant enzymes are discussed and evaluated from the perspective of structure- activity relationships.
Gurer-Orhan
Hande
H
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University, Sihhiye 06100, Ankara, Turkey.
Orhan
Hilmi
H
Suzen
Sibel
S
Püsküllü
M Orhan
MO
Buyukbingol
Erdem
E
D000975:Antioxidants / Q000138:chemical synthesis* / Q000494:pharmacology*; D001562:Benzimidazoles / Q000138:chemical synthesis* / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008954:Models, Biological; D013329:Structure-Activity Relationship; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D001562:Benzimidazoles; D006861:Hydrogen Peroxide; C031000:benzimidazole; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase