Optimization of culture variables for improving glucoamylase production by alginate-entrapped Thermomucor indicae-seudaticae using statistical methods.
Alginate-entrapped sporangiospores of Thermomucor indicae-seudaticae were used for the production of glucoamylase. The critical variables that affected glucoamylase production were identified by Plackett-Burman design (sucrose, yeast-extract, K(2)HPO(4) and asparagine) and further optimized by using a four factor central composite design (CCD) of response surface methodology (RSM). Immobilized sporangiospores secreted 41% and 60% higher glucoamylase titers in shake flasks and airlift fermenter, respectively, when the variables were used at their optimum levels (sucrose 3.0%, yeast-extract 0.2%, K(2)HPO(4) 0.1% and asparagine 0.35%). Glucoamylase production (26.3 U ml(-1)) in the optimized medium was in good agreement with the values predicted by the quadratic model (26.7 U ml(-1)), thereby confirming its validity. The enzyme production was sustainable in flasks of higher volume and also airlift fermenter, and attained a peak within 32 h in the fermenter as compared to that of 48 h in shake flasks.
Kumar
Pardeep
P
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
Satyanarayana
T
T
D000464:Alginates / Q000737:chemistry*; D019149:Bioreactors; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D009089:Mucor / Q000502:physiology*; D013172:Spores, Fungal; D013223:Statistics as Topic
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005087:Glucan 1,4-alpha-Glucosidase