BRD2 is one of BRD7-interacting proteins and its over-expression could initiate apoptosis.
BRD7 is a potential nuclear transcription regulation factor related to nasopharyngeal carcinoma (NPC). BRD2, a putative BRD7-interacting protein, has been screened from human fetal brain cDNA library by yeast two-hybrid system. This study was to further identify the interaction between BRD7 and BRD2 in mammalian cells, and to investigate the subcellular localization of BRD2, as well as the effect on the functions of cell biology. Both immunoprecipitation and subcellular colocalization were performed together to identify the interaction of BRD7 with full-length BRD2, as well as C-terminal truncated BRD2 or N-terminal truncated BRD2. GFP direct fluorescence and Hochest 33258 staining were used to investigate the cellular localization pattern of BRD2 and the roles in initiating cell apoptosis in COS7 and HNE1. The results showed that BRD7 could interact with BRD2 and the region from amino acid 430 to 798 of BRD2 was critical for the interaction of BRD2 with BRD7. BRD2 mainly localizes in nucleus in two distribution patterns, diffused and dotted, and BRD2 has distinct roles in initiating apoptosis, and the dotted distribution pattern of BRD2 in nucleus may be a morphologic marker of cell apoptosis.
Zhou
Ming
M
Cancer Research Institute, Central South University Xiang-Ya School of Medicine, Changsha, Hunan, 410078, China.
Xu
Xiao-Jie
XJ
Zhou
Hou-De
HD
Liu
Hua-Ying
HY
He
Jia-Jin
JJ
Li
Xiao-Ling
XL
Peng
Cong
C
Xiong
Wei
W
Fan
Song-Qing
SQ
Lu
Jian-Hong
JH
Ouyang
Jue
J
Shen
Shou-Rong
SR
Xiang
Bo
B
Li
Gui-Yuan
GY
D000818:Animals; D017209:Apoptosis; D019556:COS Cells; D002522:Chlorocebus aethiops; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D021381:Protein Transport; D014157:Transcription Factors; D000092468:Bromodomain Containing Proteins
D002868:Chromosomal Proteins, Non-Histone; D004247:DNA; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D014157:Transcription Factors; C098415:BRD2 protein, human; D000092468:Bromodomain Containing Proteins; C485694:BRD7 protein, human