title: Increased lactate levels impair the coagulation system--a potential contributing factor to progressive hemorrhage after traumatic brain injury.
abstract: Progressive intracerebral contusions are a major problem in the management of patients with severe traumatic brain injury that is also linked to worse outcome. Microdialysis studies have revealed that lactate levels are very high inside contusions, corresponding to significant acidosis. The current study was performed in an effort to investigate whether the lactate accumulation inside cerebral contusions may be a contributing factor to the prolonged bleeding inside contusions. We have investigated the effects of lactic acidosis on the coagulation system with rotational thromboelastometry. It was a laboratory study involving 6 healthy volunteers. Blood was drawn and the pH was adjusted by addition of lactic acid in vitro. The pH levels studied were 7.4, 7.2, 7.0, and 6.8. The pH was also readjusted to 7.4 by addition the buffer THAM to blood initially adjusted to a pH of 6.8 to study the reversibility of potential adverse effects induced by the lactic acidosis. We found the coagulation to be significantly impaired by lactic acidosis (P = 0.000l). The impairment found was reversible after correction of the acidosis by a buffer. In conclusion, we found that lactic acidosis impaired the coagulation system. The impairment caused by lactic acidosis may be one factor causing the progressive hemorrhage in posttraumatic cerebral contusions, known to have high levels of lactate and correspondingly low pH. It may also be important to consider in bleeding trauma patients.
authors.affiliation: Department of Anaesthesia and Intensive Care, Lund University Hospital, Sweden. martin.engstrom@med.lu.se
authors.forename: Martin
authors.initials: M
authors.lastname: Engstrom
authors.forename: Ulf
authors.initials: U
authors.lastname: Schott
authors.forename: Carl-Henrik
authors.initials: CH
authors.lastname: Nordstrom
authors.forename: Bertil
authors.initials: B
authors.lastname: Romner
authors.forename: Peter
authors.initials: P
authors.lastname: Reinstrup
mesh_terms: D000138:Acidosis / Q000097:blood / Q000503:physiopathology; D001777:Blood Coagulation / Q000502:physiology*; D001930:Brain Injuries / Q000150:complications / Q000503:physiopathology*; D002021:Buffers; D020202:Cerebral Hemorrhage, Traumatic / Q000209:etiology / Q000503:physiopathology*; D018450:Disease Progression; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000097:blood*; D013916:Thrombelastography; D014325:Tromethamine / Q000494:pharmacology
chemical_list: D002021:Buffers; D014325:Tromethamine; D019344:Lactic Acid