title: Expression of T-STAR gene is associated with regulation of telomerase activity in human colon cancer cell line HCT-116.
abstract: OBJECTIVE
  To investigate the effects on telomerase activity of transfection of human T-STAR gene full-length sense cDNA or partial antisense cDNA into human colon cancer cell line HCT-116.
  
  
  METHODS
  mRNA and protein expression levels of T-STAR gene were determined by RT-PCR and western blot, and telomerase activity was measured by PCR-ELISA, after transfection of T-STAR sense or antisense gene into HCT-116 cells with lipofectamine.
  
  
  RESULTS
  T-STAR gene expression was enhanced or knocked down both at mRNA and protein levels, and telomerase activity was significantly increased or decreased.
  
  
  CONCLUSIONS
  The T-STAR gene may participate in regulation of telomerase activity in human colon cancer HCT-116 cells in a parallel fashion.
authors.affiliation: Department of Disease Prevention and Health Protection, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Lian
authors.initials: L
authors.lastname: Guo
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
mesh_terms: D016373:DNA, Antisense; D018076:DNA, Complementary; D045325:HCT116 Cells; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism*; D014162:Transfection
chemical_list: D016373:DNA, Antisense; D018076:DNA, Complementary; C118777:KHDRBS3 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D019098:Telomerase