title: Preparative production and separation of 2-acetamido-2-deoxymannopyranoside-containing saccharides using borate-saturated polyolic exclusion gels.
abstract: A new separation method based on the combination of exclusion and ion exchange chromatography in borate buffer was developed. It allows semi-preparatory and preparatory separation of isobaric N-acylhexosamines (C-2 epimers) and corresponding methyl glycosides (anomers and tautomers). Three types of polyolic gels were tested for these separations. Ion-exchange HPLC was used as a rapid and reliable method for the quantification of the respective analytes. NMR studies of the interactions of N-acetylhexosamines with borate confirmed the importance of a proper stereochemical arrangement of acetamido sugars for their interactions with borate anions.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Vídenská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Petrásková
authors.forename: Andrea
authors.initials: A
authors.lastname: Charvátová
authors.forename: Vera
authors.initials: V
authors.lastname: Prikrylová
authors.forename: Veronika
authors.initials: V
authors.lastname: Kristová
authors.forename: Jana
authors.initials: J
authors.lastname: Rauvolfová
authors.forename: Ludmila
authors.initials: L
authors.lastname: Martínková
authors.forename: Jesús
authors.initials: J
authors.lastname: Jiménez-Barbero
authors.forename: Nuria
authors.initials: N
authors.lastname: Aboitiz
authors.forename: Ladislav
authors.initials: L
authors.lastname: Petrus
authors.forename: Vladimír
authors.initials: V
authors.lastname: Kren
mesh_terms: D001881:Borates / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel / Q000379:methods*; D006595:Hexosamines / Q000138:chemical synthesis* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000302:isolation & purification
chemical_list: D001881:Borates; D006595:Hexosamines; D009844:Oligosaccharides; C002022:N-acetylmannosamine