Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses.
A synthetic chimeric gene, TBI-HBS, encoding the immunogenic ENV and GAG epitopes of human immunodeficiency virus (HIV-1) and the surface protein antigen (HBsAg) of hepatitis B virus (HBV), was expressed in tomato plants. Tomato fruits containing the TBI-HBS antigen were fed to experimental mice and, on days 14 and 28 post-feeding, high levels of HIV- and HBV-specific antibodies were present in the serum and feces of the test animals. Intraperitoneal injection of a DNA vaccine directing synthesis of the same TBI-HBsAg antigen boosted the antibody response to HIV in the blood serum; however, it had no effect on the high level of antibodies produced to HBV.
Shchelkunov
Sergei N
SN
State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region, 630559, Russia.
Salyaev
Rurik K
RK
Pozdnyakov
Sergei G
SG
Rekoslavskaya
Natalia I
NI
Nesterov
Andrei E
AE
Ryzhova
Tatiana S
TS
Sumtsova
Valentina M
VM
Pakova
Natalia V
NV
Mishutina
Uliana O
UO
Kopytina
Tatiana V
TV
Hammond
Rosemarie W
RW
D000284:Administration, Oral; D017325:Hepatitis B Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism*; D006515:Hepatitis B virus / Q000187:drug effects* / Q000502:physiology; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000276:immunology*; D030821:Plants, Genetically Modified; D015202:Protein Engineering / Q000379:methods
D017325:Hepatitis B Vaccines; D010936:Plant Extracts