RAPD cluster analysis and chlorate sensitivity of some Indian isolates of Macrophomina phaseolina from sorghum and their relationships with pathogenicity.
Charcoal rot caused by Macrophomina phaseolina is an economically important disease in sorghum grown during the post rainy season in India. Variations in random amplified polymorphic DNA (RAPD) polymorphisms, chlorate sensitivity and pathogenicity were studied among sorghum isolates of M. phaseolina collected from different parts of India. RAPD data based on 14 random primers of Kit A and C (OPA and OPC) on 20 isolates showed a high degree of polymorphism (98.1%) in different isolates. UPGMA dendrogram on RAPD data produced 7 clusters at the level of 37% similarity. Isolates from the same locations showed a tendency to group closer, substantiating closer genetic relatedness. Sorghum infecting Macrophomina isolates showed a mixed response for sensitivity to potassium chlorate (120 mM). Chlorate-resistant isolates were predominant (>65% of the isolates) over sensitive isolates. Chlorate-sensitive isolates were found to be genetically closer among them than the resistant ones. For the first time it was shown that chlorate sensitivity in Macrophomina had some relations with charcoal rot severity in sorghum.
Das
I K
IK
National Research Centre for Sorghum, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, India. indrakanta_das@rediffmail.com
Fakrudin
B
B
Arora
D K
DK
D001203:Ascomycota / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000472:pathogenicity; D002704:Chlorates / Q000494:pharmacology*; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000235:genetics; D052978:Disk Diffusion Antimicrobial Tests; D007194:India; D009566:Nitrates / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D045868:Sorghum / Q000382:microbiology*; D014774:Virulence
D002704:Chlorates; D004271:DNA, Fungal; D009566:Nitrates