Functional evaluation of a novel constitutive promoter F1 of Bacillus pumilus, as a rice epiphytic strain, and construction of an efficient expression and secretion system under the control of F1.
To establish a constitutive, high-efficiency expression and secretion system for Bacillus pumilus, the function of a promoter and the abilities of three signal peptides in B. pumilus DX01 were tested. F1, cloned from the rice epiphyte B. pumilus strain DX01, had strong transcription activity and was a vegetative-phase constitutive promoter. The signal sequences of Bacillus subtilis levansucrase (sacB) and subtilisin, as well as B. pumilus DX01 RNase signal sequence could drive the secretion of E. coli beta-lactamase from B. pumilus DX01 efficiently, among which the signal sequence of B. subtilis sacB was the most effective. Likewise, they could also direct the secretion of green fluorescence protein (GFP) from DX01.
Sun
Xiaobo
X
Institute of Genetics, State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, 220 Handan Rd, Shanghai 200433, PR China.
Chen
Yunpeng
Y
Wu
Cuirong
C
Yang
Guangxiao
G
Guo
Bin
B
Shen
Daleng
D
D001407:Bacillus / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D012275:Oryza / Q000382:microbiology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D021382:Protein Sorting Signals / Q000235:genetics*; D014162:Transfection / Q000379:methods*
D021382:Protein Sorting Signals