Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues.
The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.
Churchill
Melissa J
MJ
The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia.
Gorry
Paul R
PR
Cowley
Daniel
D
Lal
Luxshimi
L
Sonza
Secondo
S
Purcell
Damian F J
DF
Thompson
Katherine A
KA
Gabuzda
Dana
D
McArthur
Justin C
JC
Pardo
Carlos A
CA
Wesselingh
Steven L
SL
D015526:AIDS Dementia Complex / Q000821:virology*; D001253:Astrocytes / Q000821:virology*; D001921:Brain / Q000821:virology*; D002467:Cell Nucleus / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis*; D015497:HIV-1 / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007834:Lasers; D008264:Macrophages / Q000821:virology*; D042282:Microdissection; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000502:physiology*; D016662:Virus Integration
D004279:DNA, Viral