Molecular markers in stored kidneys using perfluorocarbon-based preservation solution: preliminary results.
BACKGROUND
Delayed graft function (DGF) is a problem in kidney transplantation and cold ischemia has been identified as a risk factor. Perfluorocarbons (PFC) have an enhanced ability to dissolve and release oxygen. We evaluated histologically and a number of molecular changes induced by ischemia in stored kidneys with University of Wisconsin (UW) and PFC-based preservation solutions (PFC-UW).


METHODS
ACI rats were used as kidney donors. UW (control group) or PFC-UW (study group) preservation solutions were used for kidney perfusion. All kidneys were stored at 4 degrees C for 12, 24, and 36 hours. After this time, intragraft histologic evaluation as well as mRNA HO-1 and iNOS levels were also analyzed.


RESULTS
In the kidneys stored at 24 hours, mRNA HO-1 levels were elevated in the study group when compared with the control and mRNA iNOS was decreased.


CONCLUSIONS
We observed overexpression of HO-1 and underexpression of iNOS in the kidney tissue stored with PFC-UW solution at 24 hours. These preliminary data suggest that increasing oxygen delivery by PFC added to the perfusion solution triggers cytoprotective mechanism in kidney transplantation.
Maluf
D G
DG
Division of Transplant, Department of Surgery, Virginia Commonwealth University, Richmond, Virginia 23298-0248, USA. dgmaluf@vcu.edu
Mas
V R
VR
Yanek
K
K
Stone
J J
JJ
Weis
R
R
Massey
D
D
Spiess
B
B
Posner
M P
MP
Fisher
R A
RA
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D015415:Biomarkers; D005466:Fluorocarbons / Q000494:pharmacology; D005786:Gene Expression Regulation; D005978:Glutathione; D051547:Heme Oxygenase-1 / Q000235:genetics; D007328:Insulin; D007668:Kidney / Q000187:drug effects / Q000502:physiology; D008297:Male; D023421:Models, Animal; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D019188:Organ Preservation Solutions; D012333:RNA, Messenger / Q000235:genetics*; D011887:Raffinose; D051381:Rats; D011912:Rats, Inbred ACI
D015415:Biomarkers; D005466:Fluorocarbons; D007328:Insulin; D019188:Organ Preservation Solutions; D012333:RNA, Messenger; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D052247:Nitric Oxide Synthase Type II; D051547:Heme Oxygenase-1; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose