[alpha-Oxidation of 3-methyl-branched fatty acids: unraveling of a pathway].
Peroxisomes have an important role in lipid metabolism e.g. beta-oxidation of long and very long chain fatty acids, 2-methyl-branched fatty acids, dicarboxylic fatty acids, prostanoids and bile acid intermediates, and synthesis of ether lipids. Also the process of alpha-oxidation of 3-methyl-branched fatty acids, with phytanic acid (3,7,11,15-tetramethylhexadecanoic acid) as the best known example, occurs in peroxisomes. alpha-Oxidation is a process in which fatty acids are shortened by one carbon atom. The alpha-oxidation sequence of 3-methyl-branched fatty acids starts with an activation to the corresponding CoA-ester. Subsequently this acyl-CoA-ester undergoes a 2-hydroxylation by the peroxisomal phytanoyl-CoA hydroxylase (PAHX). In a third step the peroxisomal 2-hydroxyphytanoyl-CoA lyase (2-HPCL) splits the carbon carbon bond of the 2-hydroxy-intermediate into a 2-methyl(n-1)aldehyde and formyl-CoA, which is subsequently converted to formate and CO2. Finally the aldehyde is dehydrogenated by an aldehyde dehydrogenase to the corresponding acid, which, after its conversion to the acyl-CoA ester, can be a substrate for beta-oxidation. 2-HPCL is the first thiamine pyrophosphate dependent peroxisomal enzyme in mammals. Apart from 2-hydroxy-3-methylacyl-CoAs also 2-hydroxyacyl-CoAs are substrates for this enzyme. This indicates that the 2-hydroxy function but not the 3-methyl function of acyl-CoA esters is needed for 2-HPCL-activity. Long and very long chain 2-hydroxy fatty acids are constituents of brain cerebrosides and sulfatides, which mainly occur in myelin.
Casteels
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Afdeling Farmacologie, Departement Moleculaire Celbiologie, Faculteit Geneeskunde, KULeuven, Herestraat 49, B 3000 Leuven.
D000214:Acyl Coenzyme A / Q000378:metabolism; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism*; D006801:Humans; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D020675:Peroxisomes / Q000378:metabolism*
D000214:Acyl Coenzyme A; D005227:Fatty Acids