title: Morphological significance of cladosporium contaminants on materials and utensils in contact with food.
abstract: Cladosporium contaminants on materials and utensils that come into contact with food were morphologically investigated. The most common contaminants, C. cladosporioides and C. sphaerospermum, were detected on the samples. The morphological changes of the Cladosporium species were investigated by using stereoscopic, optical light, fluorescent, and scanning electron microscopes. Microscopically the Cladosporium contaminants were observed as aggregated dark brown spots, strongly pigmented, irregularly swollen, and in long chains. Using fluorescent microscopy, the Cladosporium mycelia were clearly stained with fluorescein diacetate as viable cells, but the old cells were mostly non-viable, as shown by staining with propidium iodide. The dynamics of the morphological changes showed that the penetrating mycelia were closely attached to the surface of the materials and utensils under investigation. These results provide information about the significance of Cladosporium contamination on materials and utensils in contact with food and may contribute to the control of fungal contamination.
authors.affiliation: National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya, Tokyo 158-8501, Japan.
authors.forename: Toshiko
authors.initials: T
authors.lastname: Ohta
authors.forename: Bong Joo
authors.initials: BJ
authors.lastname: Park
authors.forename: Maki
authors.initials: M
authors.lastname: Aihara
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Ri
authors.forename: Toshiko
authors.initials: T
authors.lastname: Saito
authors.forename: Takuo
authors.initials: T
authors.lastname: Sawada
authors.forename: Kosuke
authors.initials: K
authors.lastname: Takatori
mesh_terms: D002963:Cladosporium / Q000302:isolation & purification / Q000648:ultrastructure*; D003297:Cooking and Eating Utensils / Q000592:standards; D005506:Food Contamination / Q000517:prevention & control*; D005516:Food Microbiology / Q000592:standards*; D018857:Food Packaging / Q000592:standards; D008855:Microscopy, Electron, Scanning