Cell microarrays in drug discovery.
There is an increasing need for systematic cell-based assays in a high-throughput screening (HTS) format to analyze the phenotypic consequences of perturbing mammalian cells with drugs, genes, interfering RNA. Taking advantage of the recent progress in microtechnology, new cell microarrays are being developed and applied to a large range of issues in metazoan cells. This article compares different approaches and evaluates their potential use in the drug discovery process. Although still an emerging technology, cell microarrays hold great promise to optimize the efficiency:cost ratio in cell-based HTS.
Castel
David
D
CEA, DSV, DRR, Service de Génomique Fonctionnelle, 2 rue Gaston Crémieux-CP 22, 91057 Evry Cedex, France.
Pitaval
Amandine
A
Debily
Marie-Anne
MA
Gidrol
Xavier
X
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured / Q000378:metabolism; D015195:Drug Design; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D040081:Protein Array Analysis / Q000379:methods; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D046888:Tissue Array Analysis / Q000379:methods
D016326:Extracellular Matrix Proteins; D034741:RNA, Small Interfering