Postprandial serum triacylglycerols and oxidative stress in mice after consumption of fish oil, soy oil or olive oil: possible role for paraoxonase-1 triacylglycerol lipase-like activity.
OBJECTIVE
Postprandial triacylglycerols and oxidative stress responses are influenced by the type of fat consumed. We investigated the effect of individual unsaturated fatty acids or oils (fish, soy, or olive) on postprandial triglyceridemia response in association with serum resistance to oxidation and paraoxonase-1 (PON1) activity.


METHODS
Balb/C mice were supplemented with phosphate buffered saline (control), docosahexaenoic acid (omega-3), linoleic acid (omega-6), or oleic acid (omega-9; 500 microg/300 microL of phosphate buffered saline) and with fish, soy, or olive oil (300 microL); blood samples were collected 2 h after feeding.


RESULTS
Serum triacylglycerol and oxidative stress responses increased after intake of all unsaturated fatty acids and oil supplements. However, ingestion of fish oil or its major fatty acid, docosahexaenoic acid, induced the most remarkable increase in postprandial serum triacylglycerols and in the susceptibility of serum to in vitro oxidation. Serum PON1 activity was decreased by 24% after fish oil ingestion. The increase in postprandial serum susceptibility to oxidation was lower after soy oil supplementation to PON1-transgenic mice in comparison with Balb/C mice, showing that PON1 attenuates the postprandial serum oxidative response. In parallel, in PON1-transgenic mice, a decreased postprandial triacylglycerol response was noted, suggesting PON1 involvement in triacylglycerol metabolism. PON1 exhibited a triacylglycerol lipase-like activity on chylomicrons.


CONCLUSIONS
PON1 attenuates the postprandial oxidative stress response, and this could have resulted from PON1 lipase-like activity on chylomicron triacylglycerols.
Fuhrman
Bianca
B
Lipid Research Laboratory, Technion Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences and Rambam Medical Center, Haifa, Israel.
Volkova
Nina
N
Aviram
Michael
M
D000818:Animals; D030801:Animals, Genetically Modified; D043303:Aryldialkylphosphatase / Q000378:metabolism*; D002914:Chylomicrons / Q000097:blood / Q000737:chemistry*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage* / Q000378:metabolism; D005395:Fish Oils; D008049:Lipase / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D000069463:Olive Oil; D018384:Oxidative Stress / Q000187:drug effects*; D010938:Plant Oils; D019518:Postprandial Period; D011897:Random Allocation; D013024:Soybean Oil; D014280:Triglycerides / Q000097:blood* / Q000737:chemistry
D002914:Chylomicrons; D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides; D013024:Soybean Oil; D008049:Lipase; D043303:Aryldialkylphosphatase