Protective effects of beta-glucan extracted from Agaricus brasiliensis against chemically induced DNA damage in human lymphocytes.
beta-Glucans (BGs) are polysaccharides that are found in the cell walls of organisms such as bacteria, fungi, and some cereals. The objective of the present study was to investigate the genotoxic and antigenotoxic effects of BG extracted from the mushroom Agaricus brasiliensis (=Agaricus blazei Murrill ss. Heinemann). The mutagenic activity of BG was tested in single-cell gel electrophoresis assays with human peripheral lymphocytes. In addition, the protective effects against the cooked food mutagen 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and (+/-)-anti-B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE), which is the main metabolite of B[a]P, and against ROS (H(2)O(2))-induced DNA damage, were studied. The results showed that the compound itself was devoid of mutagenic activity, and that a significant dose-dependent protective effect against damage induced by hydrogen peroxide and Trp-P-2 occurred in the dose range 20-80 microg/ml. To investigate the prevention of Trp-P-2-induced DNA damage, a binding assay was carried out to determine whether BG inactivates the amine via direct binding. Since no such interactions were observed, it is likely that BG interacts with enzymes involved in the metabolism of the amine.
Angeli
J P F
JP
Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brazil.
Ribeiro
L R
LR
Gonzaga
M L C
ML
Soares
S de A
Sde A
Ricardo
M P S N
MP
Tsuboy
M S
MS
Stidl
R
R
Knasmueller
S
S
Linhares
R E
RE
Mantovani
M S
MS
D000364:Agaricus / Q000378:metabolism*; D020552:Comet Assay / Q000379:methods*; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008956:Models, Chemical; D011134:Polysaccharides / Q000737:chemistry; D011485:Protein Binding; D017382:Reactive Oxygen Species; D047071:beta-Glucans / Q000378:metabolism*
D011134:Polysaccharides; D017382:Reactive Oxygen Species; D047071:beta-Glucans; D004247:DNA; D006861:Hydrogen Peroxide