Solution structure and antiestrogenic activity of the unique C-terminal, NR-box motif-containing region of MTA1s.
Metastasis tumor-associated 1 short form (MTA1s) is a naturally occurring, alternatively spliced variant of MTA1 that functions as a repressor of estrogen receptor (ER) alpha transcriptional functions, at least in part by binding and sequestering ERalpha in the cytoplasm. A unique C-terminal 33-amino acid region containing a nuclear receptor (NR)-box motif (-LRILL-) mediates binding of MTA1s with ERalpha and is indispensable in this interaction. Here, we elucidated the solution structure of this 33-amino acid region by NMR spectroscopy. We found a predominance of the alpha-helical region toward the N-terminal region, which includes the NR-box motif. In silico docking and comparison studies showed similarities between the NR-box motif of MTA1s and a similar motif of coregulators, both in structure and mode of ERalpha binding. In MCF-7 breast cancer cells, the MTA1s peptide effectively repressed ERalpha transactivation function, as evidenced by the estrogen response element-luc assay and down-regulation of estrogen-induced genes. In mechanistic studies, we found that the antiestrogenic effects of the MTA1s peptide were due to its ability to compete with the coactivator recruitment to ERalpha. Furthermore, the peptide efficiently repressed estrogen-induced proliferation and anchorage-independent growth of MCF-7 cells. In addition, the MTA1s peptide blocked the progression of tumors formed by MCF-7 cells overexpressing an ERalpha coactivator in a xenograft-based assay. In brief, the characterization of structure and antiestrogenic activity of MTA1s peptide highlight its therapeutic potential.
Singh
Rajesh R
RR
Department of Molecular and Cellular Oncology and Experimental Therapeutics, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA.
Kaluarachchi
Kumaralal
K
Chen
Mingzhi
M
Rayala
Suresh K
SK
Balasenthil
Seetharaman
S
Ma
Jianpeng
J
Kumar
Rakesh
R
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020847:Estrogen Receptor Modulators / Q000494:pharmacology*; D004967:Estrogens / Q000378:metabolism; D005260:Female; D006655:Histone Deacetylases / Q000737:chemistry* / Q000502:physiology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009368:Neoplasm Transplantation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry* / Q000502:physiology*; D020218:Response Elements; D015534:Trans-Activators
D020847:Estrogen Receptor Modulators; D004967:Estrogens; C089320:MTA1 protein, human; D012097:Repressor Proteins; D015534:Trans-Activators; D006655:Histone Deacetylases