Effect of exposure duration of ovaries and oocytes at ambient temperature on parthenogenetic development of porcine follicular oocytes.
This study was conducted to evaluate the effects of exposing porcine ovaries to 30-33 C during transportation for 4 h and subsequently room temperature (25 C) for 6 h of storage on in vitro maturation (IVM) and subsequent parthenogenetic development of oocytes collected from the ovaries. After IVM, oocytes having a tight oopalsm membrane and no signs of degeneration were exposed to Dulbecco's phosphate-buffered saline (DPBS) with 7% ethanol (v/v) for 7 min to induce parthenogenetic activation. Moreover, we also determined whether exposure of the collected oocytes to room temperature for 1, 2 and 4 h in DPBS or porcine follicular fluid (pFF) affected parthenogenetic development. When porcine ovaries were stored after transportation, oocytes collected from the stored ovaries showed a significantly higher rate of degeneration after 65 h of IVM (58.4%) and a significantly lower rate of cleavage after parthenogenetic activation (40.1%) than oocytes collected from ovaries immediately after transportation (38.9% and 47.4%, respectively). However, there was no significant difference in developmental rates to the morula and blastocyst stages between these two groups (14.4% and 14.3%, respectively). The duration of preservation, 1, 2, and 4 h, of oocytes in DPBS did not affect parthenogenetic development. In contrast, when preserved for 4 h in pFF, the developmental rates of the oocytes were significantly decreased. This suggested that some factor(s) in follicular fluid affects the developmental rate of oocytes with the passage of time in ambient conditions. These results suggest that even after 6 h storage of ovaries, oocytes having normal morphology after IVM have the same rate of parthenogenetic development as oocytes collected from ovaries just after 4 h of transportation, except for a lower cleavage rate, and that exposure of oocytes to room temperature for 4 h in DPBS does not affect their parthenogenetic developmental competence.
Kim
Hyun-Jong
HJ
National Livestock Research Institute, RDA, Namwon, Japan.
Choi
Sun-Ho
SH
Son
Dong-Soo
DS
Cho
Sang-Rae
SR
Choe
Chang-Yong
CY
Kim
Young-Keun
YK
Han
Man-Hye
MH
Ryu
Il-Sun
IS
Kim
In-Cheul
IC
Kim
Il-Hwa
IH
Im
Kyung-Soon
KS
Nagai
Takashi
T
D000818:Animals; D005260:Female; D015571:Follicular Fluid; D009865:Oocytes / Q000254:growth & development*; D010053:Ovary / Q000502:physiology*; D010312:Parthenogenesis / Q000502:physiology*; D013552:Swine; D013696:Temperature; D013997:Time Factors