Multiplexed detection of protein cancer markers with biobarcoded nanoparticle probes.
We have developed the chemistry for preparing a universal probe and the appropriate nano- and microparticle labels that can be used to do highly selective multiplexed detection of three protein cancer markers at low-femtomolar concentration in buffer and serum media. The approach relies on a new multiplexed version of the biobarcode amplification method and offers new opportunities for studying multiple protein markers in a single sample. This could lead to new forms of disease diagnosis and monitoring disease recurrence in a variety of settings.
Stoeva
Savka I
SI
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208, USA.
Lee
Jae-Seung
JS
Smith
Jennifer E
JE
Rosen
Steven T
ST
Mirkin
Chad A
CA
D014408:Biomarkers, Tumor / Q000032:analysis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D015345:Oligonucleotide Probes; D006046:Gold; D004247:DNA