Flagellin in combination with curli fimbriae elicits an immune response in the gastrointestinal epithelial cell line HT-29.
Flagellin is the major cytokine-releasing factor when Salmonella enterica serovar Typhimurium (S. Typhimurium) infects intestinal epithelial cells. In this work it is shown that curli, an adhesive proteinaceous surface component of Enterobacteriaceae involved in biofilm formation of S. Typhimurium and Escherichia coli strains can bind flagellin and thus elicit an immune response by the intestinal epithelial cell line HT-29.
Rochon
Maike
M
Microbiology and Tumor Biology Center, Karolinska Institutet, Box 280, SE-171 77 Stockholm, Sweden.
Römling
Ute
U
D001426:Bacterial Proteins / Q000276:immunology* / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology*; D004926:Escherichia coli / Q000276:immunology* / Q000502:physiology; D005408:Flagellin / Q000276:immunology* / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000276:immunology*; D011485:Protein Binding
D001426:Bacterial Proteins; D016209:Interleukin-8; D005408:Flagellin; C077481:Crl protein, Bacteria