A highly efficient chemoenzymatic approach toward glycoprotein synthesis.
[reaction: see text] A highly efficient endoglycosidase-catalyzed synthesis of homogeneous glycoproteins is described. By using ribonuclease B as a model system, it was demonstrated that Endo-A could efficiently attach a preassembled oligosaccharide to a GlcNAc-containing protein in a regio- and stereospecific manner, when the corresponding sugar oxazoline was used as the donor substrate. The method allows the synthesis of both natural and tailor-made N-linked glycoproteins in excellent yield.
Li
Bing
B
Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland, Baltimore, 21201, USA.
Song
Haijing
H
Hauser
Steven
S
Wang
Lai-Xi
LX
D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008954:Models, Biological; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry
D006023:Glycoproteins; D009844:Oligosaccharides; D006026:Glycoside Hydrolases