S-Allylcysteine prevents the rat from 3-nitropropionic acid-induced hyperactivity, early markers of oxidative stress and mitochondrial dysfunction.
We investigated the effects of S-allylcysteine (SAC) on early behavioral alterations, striatal changes in superoxide dismutase (SOD) activity, lipid peroxidation (LP) and mitochondrial dysfunction induced by the systemic infusion of 3-nitropropionic acid (3-NPA) to rats. SAC (300 mg/kg, i.p.), given to animals 30 min before 3-NPA (30 mg/kg, i.p.), prevented the hyperkinetic pattern evoked by the toxin. In addition, 3-NPA alone produced decreased activities of manganese- (Mn-SOD) and copper/zinc-dependent superoxide dismutase (Cu,Zn-SOD), increased LP (evaluated as the formation of lipid fluorescent products) and produced mitochondrial dysfunction in the striatum (measured as decreased 3-(3,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction). In contrast, pretreatment of 3-NPA-injected rats with SAC resulted in a significant prevention of all these markers. Our findings suggest that the protective actions of SAC are related with its antioxidant properties, which in turn may be accounting for the preservation of SOD activity and primary mitochondrial tasks.
Herrera-Mundo
María N
MN
Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía, México DF, Mexico.
Silva-Adaya
Daniela
D
Maldonado
Perla D
PD
Galván-Arzate
Sonia
S
Andrés-Martínez
Leticia
L
Pérez-De La Cruz
Verónica
V
Pedraza-Chaverrí
José
J
Santamaría
Abel
A
D017109:Akathisia, Drug-Induced; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism; D001522:Behavior, Animal / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism*; D003292:Convulsants / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D003545:Cysteine / Q000031:analogs & derivatives* / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D009574:Nitro Compounds / Q000494:pharmacology*; D018384:Oxidative Stress; D011422:Propionates / Q000494:pharmacology*; D011595:Psychomotor Agitation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D000970:Antineoplastic Agents; D015415:Biomarkers; D003292:Convulsants; D007527:Isoenzymes; D009574:Nitro Compounds; D011422:Propionates; C065299:S-allylcysteine; D013482:Superoxide Dismutase; D003545:Cysteine; C015392:3-nitropropionic acid