Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening.
BacterioMatch Two-Hybrid System (Stratagene) was applied in order to identify potential human TIMAP interaction proteins in the lung. TIMAP highly expressed in endothelial cells and may be involved in endothelial cytoskeletal and barrier regulation. Seven TIMAP interacting partner proteins were identified. Four of identified proteins: cystein and glycine-rich protein 1, eukaryotic translation elongation factor 2, U5 snRNP-specific protein 116 kD, and solute carrier family 3 member 2 are involved in actin cytoskeleton organization, cell adhesion or translation and transcriptional regulation.
Adyshev
Djanybek M
DM
Department of Medicine, Division of Pulmonary and Critical Care Medicine, School of Medicine, Johns Hopkins University, 5200 Eastern Avenue, MFL Building Center Tower, Baltimore, MD 21224, USA.
Kolosova
Irina A
IA
Verin
Alexander D
AD
D004727:Endothelium / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D008565:Membrane Proteins; C459597:PPP1R16B protein, human; D011994:Recombinant Proteins