title: Synthesis of cell-permeable peptide nucleic acids and characterization of their hybridization and uptake properties.
abstract: Guanidine-based peptide nucleic acid (GPNA) monomers and oligomers containing all four natural (adenine (A), cytosine (C), guanine (G), and thymine (T)) and two unnatural (2-thiouracil (sU) and 2,6-diaminopurine (D)) nucleobases have been synthesized. Thermal denaturation study showed that GPNA oligomers containing alternate D-backbone configuration bind sequence-specifically to DNA and, when incubated with mammalian cells, localized specifically to the endoplasmic reticulum (ER).
authors.affiliation: Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Anca
authors.initials: A
authors.lastname: Dragulescu-Andrasi
authors.forename: Birendra
authors.initials: B
authors.lastname: Bhattacharya
authors.forename: Heather
authors.initials: H
authors.lastname: O'Keefe
authors.forename: Paolo
authors.initials: P
authors.lastname: Vatta
authors.forename: Jens J
authors.initials: JJ
authors.lastname: Hyldig-Nielsen
authors.forename: Danith H
authors.initials: DH
authors.lastname: Ly
mesh_terms: D002462:Cell Membrane / Q000187:drug effects*; D002463:Cell Membrane Permeability; D002851:Chromatography, High Pressure Liquid; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D044366:Transition Temperature
chemical_list: D020135:Peptide Nucleic Acids