The response of osteoblast-like cells to dexamethasone and cyclic loading.
The aim of this study was to investigate whether cyclic loading can alter the response of osteoblasts to dexamethasone. Proliferation of osteoblast-like cells (MG-63) was assessed by determining uptake of 3H-thymidine. Three doses of dexamethasone were tested: 0.001 nM, .001 nM, or 0.1 nM. Exposure to increasing levels of dexamethasone resulted in decreased proliferation of osteoblast-like cells. Although there was a slight decrease in proliferation seen in the controls (no dexamethasone) with cyclic loading, cyclic loading did not affect the response of osteoblast-like cells to dexamethasone under these test conditions. Varying loading conditions and the potential effect on metabolic and synthetic activity of the osteoblast-like cells need further study.
Jones
Lynne C
LC
Johns Hopkins Orthopaedics at Good Samaritan Hospital, 5601 Loch Raven Boulevard, Baltimore, Maryland 21239, USA. lcjones3@jhmi.edu
Yeoumans
Brian
B
Hungerford
David S
DS
Frondoza
Carmelita G
CG
D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003907:Dexamethasone / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000187:drug effects / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010507:Periodicity; D010812:Physical Stimulation; D013314:Stress, Mechanical; D016474:Weight-Bearing / Q000502:physiology
D003907:Dexamethasone