title: Production of high-content galacto-oligosaccharide by enzyme catalysis and fermentation with Kluyveromyces marxianus.
abstract: Of three beta-galactosidases from Aspergillus oryzae, Kluyveromyces lactis and Bacillus sp., used for the production of low-content galacto- oligosaccharides (GOS) from lactose, the latter produced the highest yield of trisaccharides and tetrasaccharides. GOS production was enhanced by mixing beta-galactosidase glucose oxidase. The low-content GOS syrups, produced either by beta-galactosidase alone or by the mixed enzyme system, were subjected to the fermentation by Kluyveromyces marxianus, whereby glucose, galactose, lactose and other disaccharides were depleted, resulting in up to 97% and 98% on a dry weight basis of high-content GOS with the yields of 31% and 32%, respectively.
authors.affiliation: Department of Bioengineering, Tatung University, Taipei 104, Taiwan.
authors.forename: Chao-Chun
authors.initials: CC
authors.lastname: Cheng
authors.forename: Mei-Ching
authors.initials: MC
authors.lastname: Yu
authors.forename: Tzu-Chien
authors.initials: TC
authors.lastname: Cheng
authors.forename: Dey-Chyi
authors.initials: DC
authors.lastname: Sheu
authors.forename: Kow-Jen
authors.initials: KJ
authors.lastname: Duan
authors.forename: Wei-Lun
authors.initials: WL
authors.lastname: Tai
mesh_terms: D019149:Bioreactors; D002384:Catalysis; D004187:Disaccharides / Q000378:metabolism; D005285:Fermentation / Q000502:physiology*; D005690:Galactose / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D007716:Kluyveromyces / Q000201:enzymology*; D009844:Oligosaccharides / Q000096:biosynthesis*; D019936:Probiotics; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004187:Disaccharides; D009844:Oligosaccharides; D005949:Glucose Oxidase; D001616:beta-Galactosidase; D005690:Galactose