title: Inflammatory processes triggered by TCR engagement or by local cytokine expression: differences in profiles of gene expression and infiltrating cell populations.
abstract: Immune cell-mediated inflammatory responses are triggered by TCR engagement with the target antigen, the initial event that brings about the complex sequence of events of the inflammatory process. Another form of inflammation is induced by local expression of certain cytokines. Unlike the former form of inflammation, little is known about the basic features of the cytokine-induced responses. Here, we analyzed tissue morphology, the infiltrating cells, and up-regulated, inflammation-related genes in mouse eyes in which inflammation is triggered by local transgenic (Tg) expression of cytokines and compared these features with those in eyes with experimental autoimmune uveitis (EAU), in which inflammation is initiated by engagement of TCR on sensitized T cells with their target antigen, followed by the well-defined, subsequent cytokine production. Eyes of IFN-gamma Tg mice exhibited severe, morphological changes but essentially no inflammation, and intense inflammation was found in eyes of interleukin (IL)-1 or IL-7 Tg mice. The cellular infiltration in eyes of these latter two lines of Tg mice resembled that in eyes with EAU by including many CD4 cells, but unlike in EAU, the infiltration in Tg eyes contained large proportions of B cells and only small numbers of macrophages. Real-time PCR analysis of eye RNA revealed differences among the disease models in the expression profiles of various inflammation-related genes. It is interesting that a bias toward T helper cell type 1 immunity (high IFN-gamma, RANTES/CCL5, MIG/CXCL9, and T-bet but low IL-4, IL-5, and GATA-3 transcripts) was found in EAU eyes but not in eyes of IL-1 and IL-7 Tg mice. The results thus show that similar to TCR engagement, local expression of certain cytokines triggers a complex, subsequent production of numerous inflammation-related molecules, but features of the ensued inflammatory process are determined by the triggering mechanism.
authors.affiliation: Laboratory of Immunology, National Eye Institute, NIH, Building 10, 10 Center Drive, Bethesda, MD 20892-1857, USA.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takase
authors.forename: Cheng-Rong
authors.initials: CR
authors.lastname: Yu
authors.forename: Don-Il
authors.initials: DI
authors.lastname: Ham
authors.forename: Chi-Chao
authors.initials: CC
authors.lastname: Chan
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Barbara P
authors.initials: BP
authors.lastname: Vistica
authors.forename: Eric F
authors.initials: EF
authors.lastname: Wawrousek
authors.forename: Scott K
authors.initials: SK
authors.lastname: Durum
authors.forename: Charles E
authors.initials: CE
authors.lastname: Egwuagu
authors.forename: Igal
authors.initials: I
authors.lastname: Gery
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D020869:Gene Expression Profiling; D007249:Inflammation / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014605:Uveitis / Q000276:immunology* / Q000473:pathology
chemical_list: D016207:Cytokines; D011948:Receptors, Antigen, T-Cell