title: The transcription factors Emx1 and Emx2 suppress choroid plexus development and promote neuroepithelial cell fate.
abstract: The transcription factors Emx1 and Emx2 exert important functions during development of the cerebral cortex, including its arealization. Here, we addressed their role in development of the derivatives of the midline region in the telencephalon. The center of the midline region differentiates into the choroid plexus, but little is known about its molecular specification. As we noted a lack of Emx1 or 2 expression in the midline region early in development, we interfered by misexpressing Emx1 and/or Emx2 in this region of the chick telencephalon. Ectopic expression of either Emx1 or Emx2 prior to HH 13 instructed a neuroepithelial identity in the previous midline region instead of a choroidal fate. Thus, Gli3 and Lhx2 normally restricted to the neuroepithelium expanded into the Emx misexpressing region. This was accompanied by down-regulation of Otx2 and BMP7, which implicates that these factors are essential for choroid plexus specification and differentiation. Interestingly, the region next to the ectopic Emx-misexpression then acquired a hybrid identity with some choroidal features such as Bmp7, Otx2 and Ttr gene expression, as well as some neuroepithelial features. These observations indicate that the expression levels of Emx1 and/or Emx2 restrict the prospective choroid plexus territory, a novel role of these transcription factors.
authors.affiliation: GSF-National Research Center for Environment and Health, Institute for Stem Cell Research, Ingolstädter Landstrasse 1, 85764 Neuherberg/Munich, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: von Frowein
authors.forename: Andrea
authors.initials: A
authors.lastname: Wizenmann
authors.forename: Magdalena
authors.initials: M
authors.lastname: Götz
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002831:Choroid Plexus / Q000196:embryology*; D004475:Ectoderm / Q000166:cytology / Q000502:physiology*; D018398:Homeodomain Proteins / Q000502:physiology*; D051379:Mice; D046569:Neuroepithelial Cells / Q000166:cytology* / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*; D000099260:Empty Spiracles Homeobox Proteins
chemical_list: D018398:Homeodomain Proteins; D014157:Transcription Factors; D000099260:Empty Spiracles Homeobox Proteins