Production of alpha, omega-alkanediols using Escherichia coli expressing a cytochrome P450 from Acinetobacter sp. OC4.
Our biotransformation using Escherichia coli expressing a cytochrome P450 (CYP) belonging to the CYP153A family from Acinetobacter sp. OC4 produced a great amount of 1-octanol (2,250 mg per liter) from n-octane after 24 h of incubation. This level of production is equivalent to the maximum level previously achieved in biotransformation experiments of alkanes. In addition, the initial production rate of 1-octanol was maintained throughout the entire incubation period. These results indicate that we have achieved the functional and stable expression of a CYP in E. coli for the first time. Further, our biotransformation system showed alpha,omega-diterminal oxidation activity of n-alkanes, and a large amount of 1,8-octanediol (722 mg per liter) was produced from 1-octanol after 24 h of incubation. This is the first report on the bioproduction of alpha,omega-alkanediols from n-alkanes or 1-alkanols.
Fujii
Tadashi
T
Bioresource Laboratories, Mercian Corporation, Shizuoka.
Narikawa
Tatsuya
T
Sumisa
Futoshi
F
Arisawa
Akira
A
Takeda
Koji
K
Kato
Junichi
J
D000150:Acinetobacter / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D000473:Alkanes / Q000737:chemistry* / Q000378:metabolism*; D002457:Cell Extracts; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D007536:Isomerism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009795:Octanes / Q000378:metabolism
D000473:Alkanes; D002457:Cell Extracts; D009795:Octanes; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C435228:cytochrome P-450 CYP153 (Acinetobacter); C026728:octane