Novel comparison of capillary electrophoresis versus immunoassay in the measurement of total percent carbohydrate deficient transferrin.
OBJECTIVE
Novel comparison of CDT isoforms as determined by CE with an FDA-approved immunoassay kit.


METHODS
Subjects (n=51) were categorized by drinking status based on AUDIT questionnaire responses. CDT isoform analyses by CE were compared to a commercially available, FDA-approved immunoassay. The analytical specificity of the immunoassay kit was assessed by analysis with IEF.


RESULTS
Because of the poor correlation between % CDT as measured by CE and the TIA immunoassay and between subject-reported drinking levels and results from the TIA assay, extraction column eluants from the immunoassays were analyzed by IEF for analytical specificity. % CDT by TIA included some trisialo-Tf, a non-CDT fraction, in the % CDT determination.


CONCLUSIONS
Total % CDT by CE, which separates all isoforms is more analytically specific than immunoassays because it does not include trisialo-Tf in the CDT calculation.
Strauss
Kathy A
KA
Department of Epidemiology and Preventive Medicine, University of Maryland, Baltimore, Baltimore, MD 21201, USA. kstrauss@umaryland.edu
O'Kane
Jessica
J
Cook
Janine Denis
JD
D000293:Adolescent; D000328:Adult; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D014168:Transferrin / Q000031:analogs & derivatives* / Q000032:analysis
D014168:Transferrin; C056759:carbohydrate-deficient transferrin