Anti-inflammatory effects of an ethanolic extract from Clematis mandshurica Rupr.
Clematis mandshurica Rupr (Ranunculaceae) roots are used in traditional Korean medicine to treat inflammation-related diseases. Therefore, we undertook to investigate their inhibitory effect on inflammation under non-cytotoxic conditions. The ethanolic extract of Clematis mandshurica at 100 microg/ml was found to significantly block the production of the pro-inflammatory mediators, nitric oxide (NO) and prostaglandin E(2) (PGE(2)), in lipopolysaccharide (LPS)/interferon(IFN)-gamma-stimulated mouse peritoneal macrophages, by up to 77% and 59%, respectively. In addition, it significantly inhibited cell proliferation and cytokine production (interleukin (IL)-2 and IFN-gamma) in splenocytes stimulated with Con A (concanavalin A; 5 microg/ml). Furthermore, when splenocytes from extract fed mice (200 mg/kg for 2 weeks) were activated with Con A, cell proliferation and the production of IL-2 and IFN-gamma were significantly inhibited. In addition, the extract reduced in vivo inflammation in oxazolone-induced delayed type hypersensitivity (DTH) model mice. Taken together, these data suggest that Clematis mandshurica is able to ameliorate inflammatory disease by exerting an anti-inflammatory effect in cases of proinflammatory and cell-mediated inflammation.
Park
Eun Kyung
EK
East-West Bone and Joint Research Center, College of Medicine, Kyung Hee University, Hoegi-1 dong, Dongdaemun-gu, Seoul 130-701, South Korea.
Ryu
Mi Hyun
MH
Kim
Young Hoon
YH
Lee
Yeon Ah
YA
Lee
Sang Hoon
SH
Woo
Doo Hyun
DH
Hong
Seung Jae
SJ
Han
Jung Soo
JS
Yoo
Myung Chul
MC
Yang
Hyung In
HI
Kim
Kyoung Soo
KS
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D039324:Clematis / Q000737:chemistry*; D016207:Cytokines / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry*; D005260:Female; D006967:Hypersensitivity / Q000188:drug therapy; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D008517:Phytotherapy; D028321:Plant Preparations / Q000737:chemistry* / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D028321:Plant Preparations; D009569:Nitric Oxide; D000431:Ethanol; D015232:Dinoprostone