Analysis of Mammalian sperm-egg membrane interactions during in vitro fertilization.
The interactions between egg and sperm are among the most fascinating in cell biology. These interactions include cell-cell adhesion and then membrane fusion between the two gametes. This chapter details the experimental methods used to for gamete culture and in vitro fertilization using mouse sperm and eggs.
Wortzman
Genevieve B
GB
Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins University of Bloomberg School of Public Health, Baltimore, MD, USA.
Gardner
Allison J
AJ
Evans
Janice P
JP
D000818:Animals; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D008297:Male; D008561:Membrane Fusion / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D010063:Ovum / Q000166:cytology / Q000502:physiology*; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*