Adsorption of bisphosphonate onto hydroxyapatite using a novel co-precipitation technique for bone growth enhancement.
Premature bone resorption and remodeling by osteoclasts can limit the longevity of implant fixation and recovery time. Orally administered bisphosphonates (BPs) have been used to inhibit osteoclast action at the implant/bone interface. Ideally, these should be delivered at the interface with the osteoblast-active hydroxyapatite (HA) for maximum effect. This investigation introduces a novel BP loading technique to achieve improved BP release from a simulated body fluid-grown HA (SBF-HA) with the aim of improving implant fixation. A solution co-precipitation technique incorporates the BP (pamidronate) into a thin SBF-HA coating. Surface analysis, using X-ray photoelectron spectroscopy (XPS), of the resultant coating was employed to confirm the presence of the adsorbed BP on the surface of SBF-HA. XPS analysis was also used to determine the optimal adsorption process. Osteoclast cell culture experiments confirmed the biological effectiveness of BP adsorption and proved that the pamidronate was biologically active, causing both decreased osteoclast numbers and decreased resorption.
McLeod
Kate
K
Ian Wark Research Institute, University of South Australia, Mawson Lakes, South Australia 5095, Australia.
Anderson
Gail I
GI
Dutta
Naba K
NK
Smart
Roger St C
RS
Voelcker
Nicolas H
NH
Sekel
Ron
R
Kumar
Sunil
S
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D004164:Diphosphonates / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D010010:Osteoclasts / Q000378:metabolism; D000077268:Pamidronate; D011817:Rabbits; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
D001672:Biocompatible Materials; D004164:Diphosphonates; D017886:Durapatite; D014025:Titanium; D000077268:Pamidronate