Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues.
Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts. Quantitative analysis of KLK10 and KLK11 expression levels was assessed by real-time PCR, in a cohort of 47 patients with non-small-cell lung cancer (NSCLC). Expression levels of these genes were widely distributed in the population studied. Multivariate analysis revealed a correlation between KLK10 over-expression and the squamous cell carcinoma histotype (p=0.034). There was no correlation between gene expression and patient survival. Overall, both genes behaved similarly (p<0.001). These results suggest a co-regulation of KLK10 and KLK11 expression in lung and a lack of KLK10 suppressor role in NSCLC. Finally, our findings indicate that these genes are likely involved in normal physiology processes in bronchus.
Planque
Chris
C
INSERM U618'Protéases et Vectorisation Pulmonaires', F-37000 Tours, France.
Aïnciburu
Mireille
M
Heuzé-Vourc'h
Nathalie
N
Régina
Sandra
S
de Monte
Michèle
M
Courty
Yves
Y
D017398:Alternative Splicing; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D015331:Cohort Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007610:Kallikreins / Q000235:genetics*; D008168:Lung / Q000166:cytology*; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics*; D016019:Survival Analysis
D012333:RNA, Messenger; C114880:trypsin-like serine protease; C100936:KLK10 protein, human; D007610:Kallikreins; D012697:Serine Endopeptidases