[Localization of repetitive DNA sequences in the pericentromeric heterochromatin of malarial mosquitoes of the "Anopheles maculipennis" complex].
Distribution of eight fragments of conserved repetitive DNA from pericentromeric heterochromatin of chromosome 2 of Anopheles atroparvus has been investigated by in situ hybridization on polytene chromosomes of An. atroparvus and An. messeae. We have shown that heterochromatic regions of all chromosomes both in An. atroparvus and An. messeae vary in combinations of, at least, conserved repeats. Some repeats have been found only in pericentromeric heterochromatic regions of chromosomes 2 (clones Atr2R-46a, Atr2R-73, Atr2R-85a in An. atroparvus and Atr2R-25 in An. messeae). Others have been found in two (clones Atr2R-25a and Atr2R-90 in An. atroparvus, Atr2R-25a in An. messeae) and more (clones Atr2R-118, Atr2R-136 in An. atroparvus, Atr2R-73 in An. messeae) pericentromeric heterochromatic regions of chromosomes. DNA comparison of pericentromeric heterochromatic regions of chromosomes in species of the "Anopheles maculipennis" complex is species- and chromosome-specific, due, in particular, to different maintenance of conserved repeates.
Grushko
O G
OG
Rusakova
A M
AM
Sharakhova
M V
MV
Sharakhov
I V
IV
Stegniĭ
V N
VN
D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000648:ultrastructure; D002503:Centromere / Q000648:ultrastructure*; D004247:DNA / Q000235:genetics*; D006570:Heterochromatin / Q000648:ultrastructure*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D012091:Repetitive Sequences, Nucleic Acid
D006570:Heterochromatin; D004247:DNA