Chemokines involved in protection from colitis by CD4+CD25+ regulatory T cells.
Chemokines are small proteins involved in the direction of migration of immune cells both during normal homeostasis and inflammation. Chemokines have been implicated in the pathology of many different inflammatory disorders and are therefore appealing therapeutic targets. Using a chemokine/chemokine receptor-specific gene expression profiling system of 67 genes, the authors have determined the expression profile of chemokine and chemokine receptor genes in the rectum of colitic mice and in mice that have been protected fromcolitis by CD4CD25 regulatory T cells. In mice protected from colitis, the authors found down regulation of the mRNA expression of the inflammatory chemokine receptors CCR1 and CXCR3 and their ligands CXCL9, CXCL10, CCL5, and CCL7. Also the transcripts for CCR9, CCL25, CCL17, and CXCL1 are found down regulated in protected compared with colitic animals. In addition, the authors' results suggest that CCL20 is used by CCR6 regulatory T cells in the complex process of controlling colitis because transcripts for this chemokine were expressed to a higher level in protected animals. The chemokine pathways identified in the present study may be of importance for the development of new targets for anti-inflammatory treatment strategies in human inflammatory bowel disease.
Kristensen
Nanna Ny
NN
Department of Medical Anatomy, University of Copenhagen, The Panum Institute, Copenhagen, Denmark. n.n.kristensen@mai.ku.dk
Brudzewsky
Dan
D
Gad
Monika
M
Claesson
Mogens Helweg
MH
D000818:Animals; D000938:Antigen-Presenting Cells; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D049109:Cell Proliferation; D018925:Chemokines / Q000378:metabolism*; D003092:Colitis / Q000276:immunology / Q000378:metabolism* / Q000517:prevention & control*; D006720:Homozygote; D007249:Inflammation; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis*; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D020411:Oligonucleotide Array Sequence Analysis; D013601:T-Lymphocytes / Q000378:metabolism*
D018925:Chemokines; D053645:Interleukin-2 Receptor alpha Subunit; D008024:Ligands