A high-resolution polymerase chain reaction-sequence-specific primer HLA-B*27 typing set and its application in routine HLA-B27 testing.
We designed a set of 35 polymerase chain reaction sequence-specific primers (PCR-SSP) in 29 SSP mixtures to assign 29 HLA-B*27 4-digit level alleles (B*2701-B*2721 and B*2723-B*2730). This was used in conjunction with our 41 PCR-SSP primer mixture low-resolution HLA-B typing set to fully differentiate B*27 from all other HLA-B alleles. Successful typing set validation used 521 B*27 samples covering 13 (B*2701-B*2710 and B*2712, B*2717, B*2723) alleles. The distribution of B*27 alleles was determined in a random population of 4020 local blood donors and the use of PCR-SSP B*27 typing in our routine flow cytometry-based HLA-B27/B2708 typing strategy is described.
Downing
J
J
Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Wales, United Kingdom.
Coates
E
E
Street
J
J
Hammond
L
L
Rees
T J
TJ
Pepperall
J
J
Darke
C
C
D000483:Alleles; D017931:DNA Primers; D015343:DNA Probes, HLA; D005434:Flow Cytometry; D006580:Genetic Carrier Screening / Q000379:methods; D005838:Genotype; D015796:HLA-B27 Antigen / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015343:DNA Probes, HLA; C090660:HLA-B protein, human; D015796:HLA-B27 Antigen