Drying of DNA droplets.
The evaporation kinetics of droplets containing DNA was studied, as a function of DNA concentration. Drops containing very low DNA concentrations dried by maintaining a constant base, whereas those with high concentration dried with a constant contact angle. To understand this phenomenon, the distribution of the DNA inside the droplet was measured using confocal microscopy. The results indicated that the DNA was condensed mostly on the surface of the droplets. In the case of high concentration droplets, it formed a shell, whereas isolated islands were found for droplets of low DNA concentrations. Rheologic results indicate the formation of a hydro gel in the low concentration drops, whereas phase separation between the self-assembled DNA structures and the water phase occurred at higher concentration.
Fang
Xiaohua
X
Department of Materials Science and Engineering, State University of New York at Stony Brook, New York 11794, USA. mrafailovich@notec.cc.sunysb.edu
Li
Bingquan
B
Petersen
Eric
E
Seo
Young-Soo
YS
Samuilov
Vladimir A
VA
Chen
Yong
Y
Sokolov
Jonathan C
JC
Shew
Chwen-Yang
CY
Rafailovich
Miriam H
MH
D010582:Bacteriophage lambda / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D003890:Desiccation; D018613:Microscopy, Confocal; D013499:Surface Properties
D004279:DNA, Viral