title: Acetyl-CoA synthetase from Pseudomonas putida U is the only acyl-CoA activating enzyme induced by acetate in this bacterium.
abstract: The gene (acs) encoding the acetyl-CoA synthetase (Acs) in Pseudomonas putida U has been cloned, sequenced and expressed in different microbes. The protein has been purified and characterized from a biochemical, structural and evolutionary point of view. Disruption or deletion of acs handicapped the bacterium for growth in a chemically defined medium containing acetate; this ability was regained when P. putida U was transformed with a plasmid carrying this gene. By contrast, all the acs knock-out mutants could assimilate n-alkanoic acids having a carbon length greater than C2, suggesting that other acyl-CoA activating enzymes (different from Acs) are involved in the catabolism of these compounds. However, these enzymes that can replace the function played by Acs in vivo are not induced by acetate.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, León, España.
authors.forename: Elsa
authors.initials: E
authors.lastname: Arias-Barrau
authors.forename: Elías R
authors.initials: ER
authors.lastname: Olivera
authors.forename: Angel
authors.initials: A
authors.lastname: Sandoval
authors.forename: Germán
authors.initials: G
authors.lastname: Naharro
authors.forename: José M
authors.initials: JM
authors.lastname: Luengo
mesh_terms: D000106:Acetate-CoA Ligase / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D000085:Acetates / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D010802:Phylogeny; D016958:Pseudomonas putida / Q000201:enzymology*
chemical_list: D000085:Acetates; D000214:Acyl Coenzyme A; D000106:Acetate-CoA Ligase