title: Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation.
abstract: Recombinants were generated from the ectomycorrhizal basidiomycete, Suillus grevillei, through agroinfection using a binary vector carrying the hygromycin B resistance and the autofluorescent protein, DsRed2, markers. DsRed2 was driven by a cis-regulatory region of the glyceraldeyde-3-phosphate dehydrogenase gene (gpd) from the wood-rotting basidiomycete, Coriolus hirsutus, which contains promoters and 5' gpd sequences with first through fourth exons and expressed for the first time in Suillus spp. The transformation system and recombinants expressing an autofluorescent protein may be useful in genetic analysis of the symbiosis.
authors.affiliation: Department of Applied Microbiology and Mushroom Science, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan. murmur@ffpri.affrc.go.jp.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Murata
authors.affiliation: Department of Applied Microbiology and Mushroom Science, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Sunagawa
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, 226-8501, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamazaki
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, 226-8501, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shishido
authors.affiliation: Department of Molecular Cell Biology, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Igasaki
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D038821:Mycorrhizae / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic / Q000502:physiology
chemical_list: D004271:DNA, Fungal; D008164:Luminescent Proteins; C413663:fluorescent protein 583