title: Novel comparison of capillary electrophoresis versus immunoassay in the measurement of total percent carbohydrate deficient transferrin.
abstract: OBJECTIVE
  Novel comparison of CDT isoforms as determined by CE with an FDA-approved immunoassay kit.
  
  
  METHODS
  Subjects (n=51) were categorized by drinking status based on AUDIT questionnaire responses. CDT isoform analyses by CE were compared to a commercially available, FDA-approved immunoassay. The analytical specificity of the immunoassay kit was assessed by analysis with IEF.
  
  
  RESULTS
  Because of the poor correlation between % CDT as measured by CE and the TIA immunoassay and between subject-reported drinking levels and results from the TIA assay, extraction column eluants from the immunoassays were analyzed by IEF for analytical specificity. % CDT by TIA included some trisialo-Tf, a non-CDT fraction, in the % CDT determination.
  
  
  CONCLUSIONS
  Total % CDT by CE, which separates all isoforms is more analytically specific than immunoassays because it does not include trisialo-Tf in the CDT calculation.
authors.affiliation: Department of Epidemiology and Preventive Medicine, University of Maryland, Baltimore, Baltimore, MD 21201, USA. kstrauss@umaryland.edu
authors.forename: Kathy A
authors.initials: KA
authors.lastname: Strauss
authors.forename: Jessica
authors.initials: J
authors.lastname: O'Kane
authors.forename: Janine Denis
authors.initials: JD
authors.lastname: Cook
mesh_terms: D000293:Adolescent; D000328:Adult; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D014168:Transferrin / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D014168:Transferrin; C056759:carbohydrate-deficient transferrin