Study of neonatal exposure to androgenic endocrine disruptors, testosterone and dihydrotestosterone by normal-phase HPLC.
Neonatal exposure to androgen induces developmental abnormalities in the male reproductive system. To investigate whether neonatal exposure affects spermatogenesis in juvenile and pubertal testis, Sprague-Dawley rat pups were given androgen or various androgenic endocrine disruptors by a single injection on the day of birth at concentrations ranging between 4 mm to 200 mm, and sacrificed on day 21 (juvenile) or 50 (puberty). The testes were weighed and examined histologically at each stage. Further, the metabolites of steroidogenesis were analyzed using normal-phase high-performance liquid chromatography. Neonatal exposure significantly reduced testis weights and steroidogenesis of juveniles. Neonatal exposure to testosterone and dihydrotestosterone still suppressed pubertal steroidogenesis, although testis weight was completely restored during puberty.
Kuwada
Masahiro
M
Department of Nutrition, Faculty of Human Health, Hachinohe University, 13-98 Mihono, Hachinohe, Aomori 031-8544, Japan. kuwada@hachinohe-u.ac.jp
Kawashima
Rei
R
Nakamura
Kazuo
K
Kojima
Hisako
H
Hasumi
Hideyo
H
Maki
Jun
J
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017373:Cyproterone Acetate / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000494:pharmacology*; D052244:Endocrine Disruptors / Q000494:pharmacology*; D005260:Female; D008297:Male; D051381:Rats; D013737:Testis / Q000187:drug effects* / Q000254:growth & development*; D013739:Testosterone / Q000494:pharmacology*; D014220:Trialkyltin Compounds / Q000494:pharmacology
D000728:Androgens; D052244:Endocrine Disruptors; D014220:Trialkyltin Compounds; D013196:Dihydrotestosterone; D013739:Testosterone; D017373:Cyproterone Acetate; C011559:tributyltin