Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation.
Recombinants were generated from the ectomycorrhizal basidiomycete, Suillus grevillei, through agroinfection using a binary vector carrying the hygromycin B resistance and the autofluorescent protein, DsRed2, markers. DsRed2 was driven by a cis-regulatory region of the glyceraldeyde-3-phosphate dehydrogenase gene (gpd) from the wood-rotting basidiomycete, Coriolus hirsutus, which contains promoters and 5' gpd sequences with first through fourth exons and expressed for the first time in Suillus spp. The transformation system and recombinants expressing an autofluorescent protein may be useful in genetic analysis of the symbiosis.
Murata
Hitoshi
H
Department of Applied Microbiology and Mushroom Science, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan. murmur@ffpri.affrc.go.jp.
Sunagawa
Masahide
M
Department of Applied Microbiology and Mushroom Science, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan.
Yamazaki
Takashi
T
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, 226-8501, Japan.
Shishido
Kazuo
K
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, 226-8501, Japan.
Igasaki
Tomohiro
T
Department of Molecular Cell Biology, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan.
D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D038821:Mycorrhizae / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic / Q000502:physiology
D004271:DNA, Fungal; D008164:Luminescent Proteins; C413663:fluorescent protein 583