title: [Determination of octafluoropropane content in human albumin micropheres loaded with octafluoropropane].
abstract: OBJECTIVE
  To develop a method for determinating the octafluoropropane (OFP) content in human albumin micropheres loaded with OFP.
  
  
  METHODS
  OFP content in the loaded human albumin micropheres was determined by means of gas chromatography coupled with mass spetrum/selected ion monitoring (GC-MS/SIM).
  
  
  RESULTS
  The calibration curve was linear within the concentration range of 40.92-16,368 nmol/L (R(2)=0.9999). The limit of detection was 8.18 nmol/L, and the recovery rate was (100.98-/+1.4)% (204 nmol/L), (102.63-/+2.29)% (2,046 nmol/L) and (97.5-/+2.2)% (8,184 nmol/L), respectively. The intra-day and inter-day RSDs were 0.6%-2.0% and 0.86%-2.3%, respectively.
  
  
  CONCLUSIONS
  The method of GC-MS/SIM is accurate, sensitive, precise and applicable for OFP determination in the microspheres.
authors.affiliation: Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.forename: Zhi-liang
authors.initials: ZL
authors.lastname: Chen
mesh_terms: D003287:Contrast Media / Q000032:analysis*; D004452:Echocardiography; D005466:Fluorocarbons / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008863:Microspheres; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D005466:Fluorocarbons; D012709:Serum Albumin; C042852:perflutren