The effects of intradermal and topical mitomycin C on wound healing.
OBJECTIVE
To determine the effect of intradermal and topical mitomycin C (MMC) on skin wound healing.


METHODS
A prospective, controlled study in a rat wound model performed in an academic medical center.


RESULTS
Intradermal and topical MMC application decreased wound integrity when compared with saline-treated animals at 1 week, 2 weeks, 1 month, and 6 months. Skin necrosis occurred in animals that received intradermal MMC. Hemotoxylin and eosin and immunohistochemical staining showed no consistent difference between treatment arms. Fibrosis and collagen deposition were reduced in MMC-treated wounds on trichrome staining.


CONCLUSIONS
MMC-treated wounds showed decreased wound strength compared with controls. Intradermal MMC can cause skin necrosis. Histologic findings did not always correspond with clinical data.


CONCLUSIONS
The data suggest cautious use of MMC in clinical situations when wound breaking strength is critical.
Porter
Glen T
GT
Mike O'Callaghan Federal Hospital, Nellis Air Force Base, NV 89191-6601, USA. glen.porter@nelis.af.mil
Gadre
Swarupa A
SA
Calhoun
Karen H
KH
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D005500:Follow-Up Studies; D007150:Immunohistochemistry; D007271:Injections, Intradermal; D015552:Injections, Intralesional; D008297:Male; D016685:Mitomycin / Q000008:administration & dosage*; D019384:Nucleic Acid Synthesis Inhibitors / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin