MAP kinases and heat shock-induced hormesis in human fibroblasts during serial passaging in vitro.
Adult human skin fibroblasts were exposed repeatedly to 41 degrees C or 42 degrees C heat shock (HS) for 1 h twice a week during serial passaging throughout their replicative life span. On the basis of longevity curves, cell size, and morphology, we observed that repeated mild heat shock (RMHS) at 41, degrees C had strong anti-aging hormetic effects, including 20% extension of cellular longevity. The basal levels of the MAP kinases JNK1, JNK2, and p38 increased during serial passaging, while that of ERK2 decreased. RMHS further exaggerated these effects, which suggests that age-related changes in MAP kinases may be an adaptive response for better cell survival.
Nielsen
Elise R
ER
Laboratory of Cellular Ageing, Danish Centre for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, Denmark. elisernielsen@hotmail.com
Eskildsen-Helmond
Yvonne E G
YE
Rattan
Suresh I S
SI
D000328:Adult; D018929:Cell Culture Techniques; D048430:Cell Shape; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response; D006358:Hot Temperature; D006801:Humans; D008136:Longevity / Q000502:physiology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*
D006360:Heat-Shock Proteins; D020928:Mitogen-Activated Protein Kinases