title: Comparison of the recovery of Mycobacterium bovis isolates using the BACTEC MGIT 960 system, BACTEC 460 system, and Middlebrook 7H10 and 7H11 solid media.
abstract: The BACTEC Microbacteria Growth Indicator Tube (MGIT) 960 system was evaluated to determine how it compares with the BACTEC 460 radiometric system and solid media for recovery of Mycobacterium bovis from tissue samples. A total of 506 bovine lymph node samples were collected from abattoirs in the United States and Mexico between November 2003 and September 2004. Processed samples were inoculated into an MGIT 960 tube, BACTEC 460 vial, and Middlebrook 7H10 and Middlebrook 7H11 solid media. Ziehl-Neelsen slides were prepared to check for contaminants and confirm the presence of acid-fast positive bacilli. Samples containing acid-fast bacilli were confirmed as members of the Mycobacterium tuberculosis complex by a nucleic acid assay. Niacin and nitrate biochemical tests were used to distinguish M. bovis from M. tuberculosis isolates. Statistical analyses were performed to compare recovery rate, mean time to detection, contamination rates, as well as pair-wise comparisons in each category. The results showed that the MGIT 960 system had a higher recovery rate of M. bovis (122/129) than did the BACTEC 460 (102/129) and solid media system (96/129). The average time to detection was 15.8 days for the MGIT 960 system, 28.2 days for the BACTEC 460 system, and 43.4 days for solid media. Contamination rates were 6.9% for the MGIT 960 system, 3.4% for the BACTEC 460 system, and 21.7% for solid media. These results indicate the MGIT 960 system can be used as an alternative to the BACTEC 460 system for recovering M. bovis from tissue samples.
authors.affiliation: USDA Animal and Plant Health Inspection Service, Veterinary Services, National Veterinary Services Laboratories, Mycobacteria and Brucella Section, Ames, IA, USA. Nichole.L.Hines@aphis.usda.gov
authors.forename: Nichole
authors.initials: N
authors.lastname: Hines
authors.forename: Janet B
authors.initials: JB
authors.lastname: Payeur
authors.forename: Lorraine J
authors.initials: LJ
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002417:Cattle; D003470:Culture Media; D008198:Lymph Nodes / Q000382:microbiology; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D011933:Reagent Kits, Diagnostic / Q000191:economics / Q000382:microbiology / Q000592:standards / Q000662:veterinary*; D013997:Time Factors; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D003470:Culture Media; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D011933:Reagent Kits, Diagnostic