High-resolution analysis of catechol-type siderophores using polyamide thin layer chromatography.
The iron-deficient culture supernatant of a soil bacterial strain identified as Erwinia sp. was analyzed using a new high-resolution polyamide thin layer chromatography (TLC) and a silica TLC. The results showed both TLC methods were very effective for separating simple catechol compounds such as 2,3-dihydroxybenzoic acid (2,3-DHBA) and catechol. However, in the analysis of more complicated catechol compounds or true catechol-type siderophores (conjugates of 2,3-DHBA and amino acids), the polyamide TLC had the higher resolution. Polyamide TLC analysis showed that strain S1 produced three distinct catechol-type siderophores.
Xie
Xiaojun
X
Department of Plant Nutrition, College of Natural Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China.
Wang
Jingguo
J
Yuan
Hongli
H
D002855:Chromatography, Thin Layer / Q000379:methods*; D004885:Erwinia / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000302:isolation & purification*; D009757:Nylons / Q000737:chemistry; D017262:Siderophores / Q000302:isolation & purification*
D062385:Hydroxybenzoates; D009757:Nylons; D017262:Siderophores; C009135:2,3-dihydroxybenzoic acid