Preparative production and separation of 2-acetamido-2-deoxymannopyranoside-containing saccharides using borate-saturated polyolic exclusion gels.
A new separation method based on the combination of exclusion and ion exchange chromatography in borate buffer was developed. It allows semi-preparatory and preparatory separation of isobaric N-acylhexosamines (C-2 epimers) and corresponding methyl glycosides (anomers and tautomers). Three types of polyolic gels were tested for these separations. Ion-exchange HPLC was used as a rapid and reliable method for the quantification of the respective analytes. NMR studies of the interactions of N-acetylhexosamines with borate confirmed the importance of a proper stereochemical arrangement of acetamido sugars for their interactions with borate anions.
Petrásková
Lucie
L
Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Vídenská 1083, 142 20 Prague 4, Czech Republic.
Charvátová
Andrea
A
Prikrylová
Vera
V
Kristová
Veronika
V
Rauvolfová
Jana
J
Martínková
Ludmila
L
Jiménez-Barbero
Jesús
J
Aboitiz
Nuria
N
Petrus
Ladislav
L
Kren
Vladimír
V
D001881:Borates / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel / Q000379:methods*; D006595:Hexosamines / Q000138:chemical synthesis* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000302:isolation & purification
D001881:Borates; D006595:Hexosamines; D009844:Oligosaccharides; C002022:N-acetylmannosamine