Enhancement and selective production of oligomycin through inactivation of avermectin's starter unit in Streptomyces avermitilis.
Oligomycin and its analogues, produced by Streptomyces avermitilis and other actinomycetes, are of interest for their potent and selective biological activities. PCR-mediated gene replacement, targeting bkdF, one of avermectin's starter unit encoding genes in S. avermitilis, was performed to yield an oligomycin producer, BIB0423. The engineered strain produced oligomycin A at 2.3 mg/ml compared to the wild type strain at 0.1 mg/ml. This resulting mutant was genetically stable and should be useful for the industrial production of oligomycin.
Wei
Xiong
X
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, PR China.
Yunxiang
Liang
L
Yinghua
Zheng
Z
D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000378:metabolism; D009840:Oligomycins / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
D009840:Oligomycins; D007559:Ivermectin; C019264:avermectin