title: Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG.
abstract: Autophagy, the degradation of cytoplasmic components, is an evolutionarily conserved homeostatic process involved in environmental adaptation, lifespan determination and tumour development. The tumor suppressor Beclin1 is part of the PI(3) kinase class III (PI(3)KC3) lipid-kinase complex that induces autophagy. The autophagic activity of the Beclin1-PI(3)KC3 complex, however, is suppressed by Bcl-2. Here, we report the identification of a novel coiled-coil UV irradiation resistance-associated gene (UVRAG) as a positive regulator of the Beclin1-PI(3)KC3 complex. UVRAG, a tumour suppressor candidate that is monoallelically mutated at high frequency in human colon cancers, associates with the Beclin1-Bcl-2-PI(3)KC3 multiprotein complex, where UVRAG and Beclin1 interdependently induce autophagy. UVRAG-mediated activation of the Beclin1-PI(3)KC3 complex promotes autophagy and also suppresses the proliferation and tumorigenicity of human colon cancer cells. These results identify UVRAG as an essential component of the Beclin1-PI(3)KC3 lipid kinase complex that is an important signalling checkpoint for autophagy and tumour-cell growth.
authors.affiliation: Department of Microbiology and Molecular Genetics and Tumor Virology Division, New England Primate Research Center, Harvard Medical School, 1 Pine Hill Drive, Southborough, MA 01772, USA.
authors.forename: Chengyu
authors.initials: C
authors.lastname: Liang
authors.forename: Pinghui
authors.initials: P
authors.lastname: Feng
authors.forename: Bonsu
authors.initials: B
authors.lastname: Ku
authors.forename: Iris
authors.initials: I
authors.lastname: Dotan
authors.forename: Dan
authors.initials: D
authors.lastname: Canaani
authors.forename: Byung-Ha
authors.initials: BH
authors.lastname: Oh
authors.forename: Jae U
authors.initials: JU
authors.lastname: Jung
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins; D001343:Autophagy / Q000502:physiology*; D000071186:Beclin-1; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D006801:Humans; D046911:Macromolecular Substances / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D009368:Neoplasm Transplantation; D011485:Protein Binding / Q000502:physiology; D011506:Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; C115010:Becn1 protein, mouse; D046911:Macromolecular Substances; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D025521:Tumor Suppressor Proteins; C408122:UVRAG protein, human; C512859:UVRAG protein, mouse