title: LC-MS Determination of remifentanil in maternal and neonatal plasma.
abstract: An HPLC-MS with electrospray ionization method for the determination of remifentanil in human plasma samples, pre-treated with SPE cartridge, has been developed and validated. Ionisation was performed by positive-ion electrospray and quadrupole filter mass spectrometer operated in the single ion-recording mode. Pre-treatment was performed using Waters Oasis((R)) SPE cartridges. Chromatographic separation was achieved in isocratic elution using a X-Terra C8 5 microm, 150 mm x 2.1 mm i.d. column. The mobile phase consisted of a mixture of water, methanol and acetonitrile (86:10:4, v/v/v) containing 0.1% of formic acid. The method showed to be linear in the range between 0.5 and 48.0 ng/ml, the estimated LOD is 0.18 ng/ml and the LOQ is 0.5 ng/ml. The method can be used to quantify remifentanil in plasma samples taken from adult and newborn patients in a range suitable for clinical studies.
authors.affiliation: Istituto Superiore di Sanità, Viale Regina Elena n. 299, Rome 00161, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Bossù
authors.forename: Annalisa
authors.initials: A
authors.lastname: Montinaro
authors.forename: Raffaele
authors.initials: R
authors.lastname: Lecce
authors.forename: Anna
authors.initials: A
authors.lastname: Farina
authors.forename: Ennia
authors.initials: E
authors.lastname: Suppa
authors.forename: Gaetano
authors.initials: G
authors.lastname: Draisci
authors.forename: Gianluca
authors.initials: G
authors.lastname: Gostoli
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D013058:Mass Spectrometry / Q000379:methods*; D010880:Piperidines / Q000097:blood*; D011247:Pregnancy; D000077208:Remifentanil
chemical_list: D010880:Piperidines; D000077208:Remifentanil