DNA analysis of insect iridescent virus 6: evidence for circular permutation and terminal redundancy.
DNA analysis of small insect iridovirus 6 was performed. Combined exonuclease-restriction endonuclease digestions revealed that all resulting fragments were degraded without preference for any one DNA fragment. Upon denaturation and reannealing of native linear Chilo iridescent virus DNA (158 x 10(6) daltons), duplex DNA circles of a smaller size (140 x 10(6) daltons) with protruding tails were formed.
Delius
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European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Darai
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Flügel
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RM