Direct estimation of the oxygen requirements of Achromobacter xylosoxidans for aerobic degradation of monoaromatic hydrocarbons (BTEX) in a bioscrubber.
The O2 requirements for biomass production and supplying maintenance energy demands during the degradation of both benzene and ethylbenzene by Achromobacter xylosoxidans Y234 were measured using a newly proposed technique involving a bioscrubber. Using this approach, relevant microbial parameter estimates were directly and simultaneously obtained via linear regression of pseudo steady-state data. For benzene and ethylbenzene, the biomass yield on O2, Y(X/O2), was estimated on a cell dry weight (CDW) basis as 1.96 +/- 0.25 mg CDW mgO2(-1) and 0.98 +/- 0.17 mg CDW mgO2(-1), while the specific rate of O2 consumption for maintenance, m(O2), was estimated as 0.041 +/- 0.008 mgO(2) mg CDW(-1) h(-1) and 0.053 +/- 0.022 mgO(2) mg CDW(-1) h(-1), respectively.
Nielsen
David R
DR
Department of Chemical Engineering, Queen's University, K7L 3N6 Kingston, Ontario, Canada.
McLellan
P James
PJ
Daugulis
Andrew J
AJ
D042402:Achromobacter / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D006841:Hydrocarbons, Aromatic / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
D006841:Hydrocarbons, Aromatic; D010100:Oxygen