title: The glucose clamp reveals an association between adiponectin gene polymorphisms and insulin sensitivity in obese subjects.
abstract: Results concerning the association of adiponectin gene polymorphisms (single-nucleotide polymorphisms, SNPs) with obesity, type 2 diabetes (T2DM), metabolic disorders and insulin resistance have not lead to definite conclusions. The aim of our study was to investigate a possible association between the -11391G>A and -11377C>G SNPs of adiponectin gene and measure of insulin sensitivity evaluated by the hyperinsulinemic-euglycemic clamp in a group of 'uncomplicated' obese subjects (with no associated comorbidities) (n=99, mean age 35 years) with a history of obesity lasting at least 10 years. The study of uncomplicated obese subjects, free of possible confounding factors that could interfere with insulin sensitivity, such as pharmacological treatment, provides a good model to assess insulin sensitivity per se. We observed that subjects homozygous for the G allele at locus -11391 had lower M (mg/kg min)/fat-free mass (FFM) index and adiponectin levels compared to subjects with GA+AA genotypes (P=0.002 and P=0.03, respectively) and subjects carrying the -11377G variant had lower M (mg/kg min)/FFM index and adiponectin levels compared to noncarriers (P=0.003 and P=0.03, respectively). Our results imply that the two promoter SNPs, -11391G>A and -11377C>G, of the adiponectin gene are associated with a reduced insulin sensitivity evaluated by hyperinsulinemic-euglycemic clamp in obese subjects.
authors.affiliation: Endocrinology, Department of Clinical Sciences, Università di Roma La Sapienza, Viale del Policlinico 155, Rome, Italy. raffaella.buzzetti@uniroma1.it
authors.forename: R
authors.initials: R
authors.lastname: Buzzetti
authors.forename: A
authors.initials: A
authors.lastname: Petrone
authors.forename: S
authors.initials: S
authors.lastname: Zavarella
authors.forename: S
authors.initials: S
authors.lastname: Zampetti
authors.forename: M
authors.initials: M
authors.lastname: Spoletini
authors.forename: S
authors.initials: S
authors.lastname: Potenziani
authors.forename: G
authors.initials: G
authors.lastname: Leto
authors.forename: J
authors.initials: J
authors.lastname: Osborn
authors.forename: F
authors.initials: F
authors.lastname: Leonetti
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000235:genetics*; D000328:Adult; D000483:Alleles; D005260:Female; D015309:Glucose Clamp Technique / Q000379:methods; D005951:Glucose Tolerance Test; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D052242:Adiponectin