In vivo modulation of epidermal growth factor receptor phosphorylation in mice expressing different gangliosides.
We studied in this work the in vivo phosphorylation of the epidermal growth factor receptor (EGFr) in skin from knockout mice lacking different ganglioside glycosyltransferases. Results show an enhancement of EGFr phosphorylation, after EGF stimulation, in skin from Sial-T2 knockout and Sial-T2/GalNAc-T double knockout mice as compared with wild-type and Sial-T1 knockout mice. Qualitative analysis of ganglioside composition in mice skin suggest that the increase of EGFr phosphorylation observed in skin from Sial-T2 knockout and Sial-T2/GalNAc-T double knockout mice in response to EGF might not be primary attributed to the expression of GD3 or a-series gangliosides in mice skin. These studies provide, for the first time, an approach for studying the molecular mechanisms involved in the in vivo regulation of EGFr function by gangliosides.
Daniotti
Jose L
JL
Departamento de Química Biológica, Facultad de Ciencias Químicas, Centro de Investigaciones en Química Biológica de Córdoba, CIQUIBIC (UNC-CONICET), Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina. daniotti@dqb.fcq.unc.edu.ar
Crespo
Pilar M
PM
Yamashita
Tadashi
T
D000818:Animals; D000831:Animals, Newborn; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism
D005732:Gangliosides; D004815:Epidermal Growth Factor; D017350:N-Acetylgalactosaminyltransferases; D012799:Sialyltransferases; C021343:haematoside synthetase; D066246:ErbB Receptors