Prolongation of rat intestinal allograft survival by administration of donor interleukin-12 p35-silenced bone marrow-derived dendritic cells.
Severe graft rejection remains an important obstacle in intestinal transplantation. In this study, we demonstrated that intravenous injection of interleukin (IL)-12 p35 siRNA-transfected dendritic cells (DCs) into recipient rats prolonged the survival of intestinal allografts. Serum IL-2 and interferon-gamma levels in the IL-12 p35 siRNA-transfected DCs treatment group were both significantly lower than in control groups at 7 days after transplantation. Further study is required to investigate the operative pathways and to optimize the strategy targeting dendritic cells to induce tolerance to intestinal allografts.
Xu
H
H
Nanjing Medical University, Nanjing, People's Republic of China.
Chen
T
T
Wang
H Q
HQ
Ji
M J
MJ
Zhu
X
X
Wu
W X
WX
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D020868:Gene Silencing; D006085:Graft Survival / Q000502:physiology*; D007108:Immune Tolerance / Q000276:immunology*; D018664:Interleukin-12 / Q000235:genetics*; D007422:Intestines / Q000637:transplantation*; D023421:Models, Animal; D012313:RNA / Q000235:genetics; D016372:RNA, Antisense / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D014162:Transfection; D014184:Transplantation, Homologous / Q000502:physiology*
D016372:RNA, Antisense; D034741:RNA, Small Interfering; D018664:Interleukin-12; D012313:RNA