Genomic approaches for identifying DNA damage response pathways in S. cerevisiae.
DNA damage response pathways have been studied extensively in the budding yeast Saccharomyces cerevisiae, yet new genes with roles in the DNA damage response are still being identified. In this chapter we describe the use of functional genomic approaches in the identification of DNA damage response genes and pathways. These techniques take advantage of the S. cerevisiae gene deletion mutant collection, either as an ordered array or as a pool, and can be automated for high throughput.
Chang
Michael
M
Biochemistry, University of Toronto, Toronto, Ontario, Canada.
Parsons
Ainslie B
AB
Sheikh
Bilal H
BH
Boone
Charles
C
Brown
Grant W
GW
D001483:Base Sequence; D004249:DNA Damage; D017931:DNA Primers; D017353:Gene Deletion; D017930:Genes, Reporter; D016681:Genome, Fungal; D009154:Mutation; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D017931:DNA Primers