title: Influence of hypercalcic and/or hyperoxalic diet on calcium oxalate renal stone formation in rats.
abstract: OBJECTIVE
  To test whether increasing dietary calcium intake prevents calcium oxalate stone formation when the diet is oxalate-rich. Material and methods. Four groups, eight rats in each, were subjected to a lithogenic diet by the addition of 0.5% ethylene glycol to drinking water for 3 weeks. The first group, used as a control, simultaneously received a standard diet. The second group was supplemented with calcium at 500 mg/100 g of diet and the third group with oxalate at 3 g/100 g of diet. The diet given to the last group was supplemented with similar doses of calcium and oxalate. One day before the end of treatment, each animal was placed in a metabolic cage to collect 24-h urine samples and determine urinary parameters. The kidneys were removed to determine calcium oxalate deposits and for histological examination.
  
  
  RESULTS
  The number of calcium oxalate crystals in renal tissue was highest in the oxalate group and calcium oxalate deposits were also found to be elevated in this group. Hyperoxaluria and hypocitraturia, induced by a oxalate-rich diet, seemed to be the major causes contributing to aggravated renal stone formation. The protective effect of dietary calcium supplementation, which was clear in the calcium + oxalate group, was probably due to intestinal binding of oxalate by calcium, thereby reducing urinary oxalate excretion.
  
  
  CONCLUSIONS
  Increased dietary calcium intake can prevent calcium oxalate stone formation only when the diet is oxalate-rich.
authors.affiliation: 04/UR/09-03 Faculté de Médecine de Monastir, Tunisia. bardaouimourad@yahoo.fr
authors.forename: Mourad
authors.initials: M
authors.lastname: Bardouri
authors.forename: Bardaoui
authors.initials: B
authors.lastname: Mourad
authors.forename: Fadwa
authors.initials: F
authors.lastname: Neffati
authors.forename: Neffati
authors.initials: N
authors.lastname: Fadwa
authors.forename: Mounir
authors.initials: M
authors.lastname: Trimeche
authors.forename: Trimeche
authors.initials: T
authors.lastname: Mounir
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Elhani
authors.forename: Elhani
authors.initials: E
authors.lastname: Abdelhamid
authors.forename: Mohamed
authors.initials: M
authors.lastname: Fadhel Najjar
authors.forename: Najjar
authors.initials: N
authors.lastname: Mohamed Fadhel
authors.forename: Rachid
authors.initials: R
authors.lastname: Sakly
authors.forename: Sakly
authors.initials: S
authors.lastname: Rachid
mesh_terms: D000818:Animals; D002129:Calcium Oxalate / Q000737:chemistry / Q000652:urine*; D002136:Calcium, Dietary / Q000627:therapeutic use*; D003460:Crystallization; D004032:Diet; D007668:Kidney / Q000473:pathology; D007669:Kidney Calculi / Q000737:chemistry / Q000517:prevention & control* / Q000652:urine; D010070:Oxalates; D051381:Rats
chemical_list: D002136:Calcium, Dietary; D010070:Oxalates; D002129:Calcium Oxalate