Compensatory effect of the minor Streptomyces lividans type I signal peptidases on the SipY major signal peptidase deficiency as determined by extracellular proteome analysis.
The developmentally complex bacterium Streptomyces lividans has the ability to produce and secrete a significant amount of protein and possesses four different type I signal peptidase genes (sipW, sipX, sipY and sipZ) that are unusually clustered in its chromosome. 2-DE and subsequent MS of extracellular proteins showed that proteins with typical export signals for type I and type II signal peptidases are the main components of the S. lividans secretome. Secretion of extracellular proteins is severely reduced in a strain deficient in the major type I signal peptidase (SipY). This deficiency was efficiently compensated by complementation with any of the other three signal peptidases as deduced from a comparison of the corresponding 2-D PAGE patterns with that of the wild-type strain.
Escutia
Marta R
MR
Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, Cantoblanco, Madrid, Spain.
Val
Gema
G
Palacín
Arantxa
A
Geukens
Nick
N
Anné
Jozef
J
Mellado
Rafael P
RP
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D012697:Serine Endopeptidases / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D048170:Streptomyces lividans / Q000201:enzymology*; D013379:Substrate Specificity
D001426:Bacterial Proteins; D008565:Membrane Proteins; D020543:Proteome; D012697:Serine Endopeptidases; C026515:type I signal peptidase