Obesity-related leptin receptor polymorphisms and gallstones disease.
OBJECTIVE
Investigate the association between polymorphisms in the leptin receptor gene associated with obesity and gallstone disease.


METHODS
We conducted a cross-sectional study, carried out at a tertiary setting.


METHODS
We enrolled 97 subjects, comprising 54 subjects with gallstones (cases) and 43 controls (without gallstones).


METHODS
Diet was assessed using a validated questionnaire for the Mexican population. Body mass index, waist circumference, serum glucose,insulin, leptin, lipids and lipoproteins levels were measured. Insulin resistance was calculated by HOMA-IR. Genomic DNA was isolated from lymphoblastoid cells, and Q223R and K656N polymorphisms in the leptin receptor gene were typed using polymerase chain reaction. Unconditional univariate logistic regression analysis was conducted to estimate the probability of gallstone disease associated with the polymorphisms as main effect.


RESULTS
Cases were different in gender(40.74% males in cases vs 74.41% in controls; p < 0.001), older (49.74 vs 44.83 years; p < 0.05), and had more body fat (32.34% vs 28.14%; p = 0.01). Individuals carrying the polymorphism Q223R exhibited a higher BMI (28.44 +/- 6.6 kg/m2 vs 25.94 +/- 3.67 kg/m2, p < 0.05) and waist circumference (96.7 +/- 16.39 cm vs 89.2 +/- 11.05 cm, p < 0.05). In univariate analysis, we did not observe a relation between the presence of a R223 or N656 genotype and gallstone disease in our population (OR = 0.78, 95% CI 0.35-1.73).


CONCLUSIONS
Obesity-related leptin receptor polymorphisms are not associated with gallstones disease.
Méndez-Sánchez
Nahum
N
Biomedical Research Department & Liver Unit, Medica Sur Clinic & Foundation, Mexico City, Mexico. nmendez@medicasur.org.mx
Bermejo-Martínez
Luisa
L
Chávez-Tapia
Norberto C
NC
Zamora-Valdés
Daniel
D
Sánchez-Lara
Karla
K
Uribe-Ramos
Martha H
MH
Ponciano-Rodríguez
Guadalupe
G
Baptista-González
Hector A
HA
Ramos
Martha H
MH
Uribe
Misael
M
D000328:Adult; D005260:Female; D042882:Gallstones / Q000150:complications* / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D016015:Logistic Models; D008297:Male; D008800:Mexico; D008875:Middle Aged; D009765:Obesity / Q000150:complications* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics*; D054411:Receptors, Leptin
C515448:LEPR protein, human; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin