title: Temporal expression of estrogen receptor alpha in rat bone marrow mesenchymal stem cells.
abstract: Estrogen responsiveness of bone formation is mediated by the estrogen receptor alpha (ERalpha) in osteoblastic lineage. As osteoblasts arise from the multipotent bone marrow stromal (mesenchymal) cells, this study was undertaken to observe the ERalpha in primary female adult rat bone marrow mesenchymal stem cells (BMSCs). The ERalpha was localized using immunocytochemical analysis in identified primary BMSCs. Then, using real-time PCR analysis, we measured the expression of ERalpha messenger RNA (mRNA) in BMSCs. ERalpha transcripts showed different trends between untreated cultures (control group) and osteogenic-induced cultures (treated group). In the control group, ERalpha mRNA climbed at peak levels at a confluence stage and decreased until day 20, whereas, in the treated group, the ERalpha mRNA kept climbing from a low level until day 20. Thus, the observed developmental expression of ERalpha mRNA correlates with progressive BMSCs growth and osteogenic differentiation and BMSCs may be a primary target cell for estrogen in maintaining bone formation.
authors.affiliation: Department of Orthodontics, College of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Yu
authors.forename: Hui-hong
authors.initials: HH
authors.lastname: Zhai
authors.forename: Qing-tao
authors.initials: QT
authors.lastname: Zhao
authors.forename: Jin-wu
authors.initials: JW
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Shu
authors.forename: De-qiang
authors.initials: DQ
authors.lastname: Li
authors.forename: Da-yong
authors.initials: DY
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Dong
authors.forename: Yin
authors.initials: Y
authors.lastname: Ding
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D047628:Estrogen Receptor alpha