Enhancement of sapovirus recombinant capsid protein expression in insect cells.
Human sapovirus (SaV) is uncultivable, but expression of the recombinant capsid protein (rVP1) in insect cells results in the formation of virus-like particles (VLPs) that are morphologically similar to the native viruses. However, the SaV rVP1 expression levels are considerably low. We have found that inclusions of short foreign nucleotide sequences inserted directly upstream from the predicted rVP1 AUG start codon lead to increased yield of VLPs. This method allowed us to express a SaV rVP1, which could not have been expressed to measurable or practical levels otherwise.
Hansman
Grant S
GS
Department of Virology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-murayama, Tokyo 208-0011, Japan. ghansman@nih.go.jp
Oka
Tomoichiro
T
Katayama
Kazuhiko
K
Takeda
Naokazu
N
D000818:Animals; D002213:Capsid / Q000648:ultrastructure; D036022:Capsid Proteins / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D018387:Codon, Initiator; D015870:Gene Expression; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D016254:Mutagenesis, Insertional; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D029341:Sapovirus / Q000235:genetics / Q000648:ultrastructure; D018411:Spodoptera / Q000166:cytology
D036022:Capsid Proteins; D018387:Codon, Initiator; D011994:Recombinant Proteins