title: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii. Functional consequences for glyceraldehyde-3-phosphate dehydrogenase.
abstract: The 8.5 kDa chloroplast protein CP12 is essential for assembly of the phosphoribulokinase/glyceraldehyde-3-phosphate dehydrogenase (GAPDH) complex from Chlamydomonas reinhardtii. After reduction of this complex with thioredoxin, phosphoribulokinase is released but CP12 remains tightly associated with GAPDH and downregulates its NADPH-dependent activity. We show that only incubation with reduced thioredoxin and the GAPDH substrate 1,3-bisphosphoglycerate leads to dissociation of the GAPDH/CP12 complex. Consequently, a significant twofold increase in the NADPH-dependent activity of GAPDH was observed. 1,3-Bisphosphoglycerate or reduced thioredoxin alone weaken the association, causing a smaller increase in GAPDH activity. CP12 thus behaves as a negative regulator of GAPDH activity. A mutant lacking the C-terminal disulfide bridge is unable to interact with GAPDH, whereas absence of the N-terminal disulfide bridge does not prevent the association with GAPDH. Trypsin-protection experiments indicated that GAPDH may be also bound to the central alpha-helix of CP12 which includes residues at position 36 (D) and 39 (E). Mutants of CP12 (D36A, E39A and E39K) but not D36K, reconstituted the GAPDH/CP12 complex. Although the dissociation constants measured by surface plasmon resonance were 2.5-75-fold higher with these mutants than with wild-type CP12 and GAPDH, they remained low. For the D36K mutation, we calculated a 7 kcal.mol(-1) destabilizing effect, which may correspond to loss of the stabilizing effect of an ionic bond for the interaction between GAPDH and CP12. It thus suggests that electrostatic forces are responsible for the interaction between GAPDH and CP12.
authors.affiliation: Institut Jacques Monod, CNRS-Universités Paris VI et Paris VII, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Lebreton
authors.forename: Simona
authors.initials: S
authors.lastname: Andreescu
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Graciet
authors.forename: Brigitte
authors.initials: B
authors.lastname: Gontero
mesh_terms: D000818:Animals; D001665:Binding Sites; D016825:Chlamydomonas reinhardtii / Q000737:chemistry* / Q000201:enzymology*; D002736:Chloroplasts / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008958:Models, Molecular; D008970:Molecular Weight; D009154:Mutation; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D021122:Protein Subunits / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D055672:Static Electricity; D020349:Surface Plasmon Resonance; D013879:Thioredoxins / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
chemical_list: D004163:Diphosphoglyceric Acids; D010446:Peptide Fragments; D010940:Plant Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; C015891:glycerate 1,3-biphosphate; D013879:Thioredoxins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022390:phosphoribulokinase; D014357:Trypsin; D003545:Cysteine