title: Molecular markers in stored kidneys using perfluorocarbon-based preservation solution: preliminary results.
abstract: BACKGROUND
  Delayed graft function (DGF) is a problem in kidney transplantation and cold ischemia has been identified as a risk factor. Perfluorocarbons (PFC) have an enhanced ability to dissolve and release oxygen. We evaluated histologically and a number of molecular changes induced by ischemia in stored kidneys with University of Wisconsin (UW) and PFC-based preservation solutions (PFC-UW).
  
  
  METHODS
  ACI rats were used as kidney donors. UW (control group) or PFC-UW (study group) preservation solutions were used for kidney perfusion. All kidneys were stored at 4 degrees C for 12, 24, and 36 hours. After this time, intragraft histologic evaluation as well as mRNA HO-1 and iNOS levels were also analyzed.
  
  
  RESULTS
  In the kidneys stored at 24 hours, mRNA HO-1 levels were elevated in the study group when compared with the control and mRNA iNOS was decreased.
  
  
  CONCLUSIONS
  We observed overexpression of HO-1 and underexpression of iNOS in the kidney tissue stored with PFC-UW solution at 24 hours. These preliminary data suggest that increasing oxygen delivery by PFC added to the perfusion solution triggers cytoprotective mechanism in kidney transplantation.
authors.affiliation: Division of Transplant, Department of Surgery, Virginia Commonwealth University, Richmond, Virginia 23298-0248, USA. dgmaluf@vcu.edu
authors.forename: D G
authors.initials: DG
authors.lastname: Maluf
authors.forename: V R
authors.initials: VR
authors.lastname: Mas
authors.forename: K
authors.initials: K
authors.lastname: Yanek
authors.forename: J J
authors.initials: JJ
authors.lastname: Stone
authors.forename: R
authors.initials: R
authors.lastname: Weis
authors.forename: D
authors.initials: D
authors.lastname: Massey
authors.forename: B
authors.initials: B
authors.lastname: Spiess
authors.forename: M P
authors.initials: MP
authors.lastname: Posner
authors.forename: R A
authors.initials: RA
authors.lastname: Fisher
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D015415:Biomarkers; D005466:Fluorocarbons / Q000494:pharmacology; D005786:Gene Expression Regulation; D005978:Glutathione; D051547:Heme Oxygenase-1 / Q000235:genetics; D007328:Insulin; D007668:Kidney / Q000187:drug effects / Q000502:physiology; D008297:Male; D023421:Models, Animal; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D019188:Organ Preservation Solutions; D012333:RNA, Messenger / Q000235:genetics*; D011887:Raffinose; D051381:Rats; D011912:Rats, Inbred ACI
chemical_list: D015415:Biomarkers; D005466:Fluorocarbons; D007328:Insulin; D019188:Organ Preservation Solutions; D012333:RNA, Messenger; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D052247:Nitric Oxide Synthase Type II; D051547:Heme Oxygenase-1; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose