title: Induction of cellular immunity against hair follicle melanocyte causes alopecia.
abstract: Alopecia areata (AA) is generally regarded as an organ-specific autoimmune disease. Although it has been hypothesized that the autoimmunity is mediated by T cells and that hair follicle melanocyte is one of the targets, definitive evidence is lacking. We here demonstrate that AA-like lesions can be induced in mice by inducing CD8(+) T-cell-mediated immunity to hair follicle melanocytes. We found that hair loss was induced in mice-bearing interleukin-12-producing B16 melanoma cells by the depletion of CD4(+) T cells, accompanied by vitiligo-like coat color change. The alopecic lesions varied in size from pachy to extensive. In many instances, hair loss developed and was followed by the regrowth of white hairs. Histological analysis revealed that mononuclear cells infiltrated in and around the bulb region of hair follicles. Furthermore, immunohistochemical examination clearly showed the intra-follicular infiltration of CD8(+) T cells. Neither the vitiligo-like coat color nor AA-like lesions were induced when CD8(+) T cells were codepleted. These observations indicate that the induction of CD8(+) T-cell-mediated immunity against hair follicle melanocytes causes alopecia. It is thought that there are many types of AA with different mechanisms, targets etc. Although hair follicle melanocytes have long been thought to be one of the targets of AA, evidence to support the hypothesis is sparse. Therefore, we believe that our observation is significant to support the hypothesis.
authors.affiliation: Department of Dermatology, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. hnagai@med.kobe-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagai
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Oniki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Oka
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Horikawa
authors.forename: Chikako
authors.initials: C
authors.lastname: Nishigori
mesh_terms: D000505:Alopecia / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D018859:Hair Follicle / Q000166:cytology / Q000276:immunology* / Q000473:pathology; D007111:Immunity, Cellular / Q000502:physiology; D008544:Melanocytes / Q000276:immunology*; D008546:Melanoma, Experimental / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology