Rapid solid-phase peptide synthesis using thermal and controlled microwave irradiation.
A rapid and efficient microwave-assisted solid-phase synthesis method is described for the preparation of the nonapeptide WDTVRISFK, using conventional Fmoc/Bu(t) orthogonal protection strategy. The synthesis protocol is based on the use of cycles of pulsed microwave irradiation with intermittent cooling of the reaction during the removal of the Fmoc protecting group and during the coupling. The desired nonapeptide was obtained in highest yield and purity by employing MicroKan technology. The chemical reactions were carried out in a single-mode microwave reactor, equipped with a fiber-optic probe to monitor the reaction temperature continuously.
Bacsa
Bernadett
B
Institute of Chemistry, Eötvös Loránd University, H-1518 Budapest 112, Hungary.
Desai
Bimbisar
B
Dibó
Gábor
G
Kappe
C Oliver
CO
D001671:Biochemistry / Q000379:methods*; D008872:Microwaves; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
D010455:Peptides