Characteristics of Ca2+-exocytosis coupling in isolated mouse pancreatic beta cells.
OBJECTIVE
To characterize Ca2+-stimulated exocytosis in isolated mouse pancreatic beta cells.


METHODS
An improved method was described for isolation of mouse pancreatic beta cells by collagenase P. The Ca2+ channel current and the membrane capacitance were examined by using the whole-cell patch clamp recording technique.


RESULTS
Using depolarization and flash photolysis of caged Ca2+ to induce Ca2+-dependent exocytosis in beta cell from KM mouse, we have explored the characteristics of the Ca2+ channel current and the relationship between Ca2+ signals and exocytosis. The averaged peak Ca2+ current measured at +20 mV was -60+/-6 pA (n=13).


CONCLUSIONS
We characterized three kinetically different pools of vesicles in mouse pancreatic beta cells, namely an immediately releasable pool, a readily releasable pool, and a reserve pool.
Ge
Qian
Q
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Dong
Yong-ming
YM
Hu
Zhi-tao
ZT
Wu
Zheng-xing
ZX
Xu
Tao
T
D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D020013:Calcium Signaling; D002478:Cells, Cultured; D004705:Endocytosis / Q000502:physiology; D005089:Exocytosis / Q000502:physiology*; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice
D020746:Calcium Channels, L-Type; D002118:Calcium