Analyses of phosphorylation events in the rubella virus capsid protein: role in early replication events.
The Rubella virus capsid protein is phosphorylated prior to virus assembly. Our previous data are consistent with a model in which dynamic phosphorylation of the capsid regulates its RNA binding activity and, in turn, nucleocapsid assembly. In the present study, the process of capsid phosphorylation was examined in further detail. We show that phosphorylation of serine 46 in the RNA binding region of the capsid is required to trigger phosphorylation of additional amino acid residues that include threonine 47. This residue likely plays a direct role in regulating the binding of genomic RNA to the capsid. We also provide evidence which suggests that the capsid is dephosphorylated prior to or during virus budding. Finally, whereas the phosphorylation state of the capsid does not directly influence the rate of synthesis of viral RNA and proteins or the assembly and secretion of virions, the presence of phosphate on the capsid is critical for early events in virus replication, most likely the uncoating of virions and/or disassembly of nucleocapsids.
Law
LokMan J
LJ
Department of Cell Biology, 5-14 Medical Sciences Building, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.
Ilkow
Carolina S
CS
Tzeng
Wen-Pin
WP
Rawluk
Matthew
M
Stuart
David T
DT
Frey
Teryl K
TK
Hobman
Tom C
TC
D000818:Animals; D019556:COS Cells; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins / Q000378:metabolism*; D002522:Chlorocebus aethiops; D006224:Cricetinae; D010766:Phosphorylation; D011485:Protein Binding; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000096:biosynthesis; D012412:Rubella virus / Q000502:physiology*; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D014709:Vero Cells; D019065:Virus Assembly / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology*
D036022:Capsid Proteins; D012367:RNA, Viral; D013912:Threonine; D012694:Serine