title: Development of restricted-access media supports and their application to the direct analysis of biological fluid samples via high-performance liquid chromatography.
abstract: A quick overview of published methods for analyzing compounds in complex biological samples reveals that the most difficult step is the clean-up or extraction of a required compound from the matrix. The strategy required to analyze exogenous compounds in biological fluids depends greatly upon the nature of the compound and upon the biomatrix. Coupled-column separation using restricted-access media as the first dimension in order to exclude macromolecules and retain micromolecules has been successfully used for a number of biological fluids. This paper presents the history of the development of restricted-access media supports and of their application to the direct injection of biological fluid samples in high-performance liquid chromatography.
authors.affiliation: Departamento de Química, Universidade Federal de São Carlos, Cx. Postal 676, São Carlos, SP, 13565-905, Brazil.
authors.forename: N M
authors.initials: NM
authors.lastname: Cassiano
authors.forename: V V
authors.initials: VV
authors.lastname: Lima
authors.forename: R V
authors.initials: RV
authors.lastname: Oliveira
authors.forename: A C
authors.initials: AC
authors.lastname: de Pietro
authors.forename: Q B
authors.initials: QB
authors.lastname: Cass
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods / Q000592:standards; D001826:Body Fluids; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D002624:Chemistry, Clinical / Q000379:methods / Q000592:standards; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D019411:Clinical Laboratory Techniques / Q000592:standards; D006801:Humans; D016482:Urinalysis / Q000379:methods