Induction of human UDP-glucuronosyltransferase 1A1 by cortisol-GR.
During the course of the study of UGT1A1 induction by bilirubin, we could not detect the induction of the reporter gene (-3174/+14) of human UGT1A1 in HepG2 by bilirubin (Mol. Biol. Rep. 31: 151-158 (2004)). In this report, we show the finding of the induction of the reporter gene of UGT1A1 by cortisol at 1 microM, a major natural cortico-steroid, with human glucocorticoid receptor (GR). RU486 of a typical GR antagonist at 10 microM inhibited the induction by cortisol from 5.9- to 1.8-fold. This result indicates that the induction by cortisol-GR is dependence on ligand-binding. This induction is caused by the UGT reporter gene itself, from the results of noinduction with control vector pGL2 (equal to pGV-C) in the presence of cortisol-GR. We confirmed that the induction of the reporter gene by cortisol is dependent on the position of proximal element (-97/-53) of UGT1A1. From this result, we concluded that the increase of corticosteroid in neonates must induce the elevation of UGT1A1 after birth and prevent jaundice. With the study of induction by corisol, we studied the influence of co-expression of PXR (pregnenolone xenobiotic receptor) with the UGT1A1 reporter gene and we could not find the induction of UGT1A1 expression in the presence of dexamethasone, rifampicin, or pregnenolone 16alpha-carbonitrile of the PXR ligands. These results suggest that the induction of UGT1A1 expression by GR is not mediated by PXR, unlike the induction of CYP3A4 through PXR.
Usui
Toru
T
Department of Drug Metabolism and Disposition, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan.
Kuno
Takuya
T
Mizutani
Takaharu
T
D001483:Base Sequence; D002460:Cell Line; D004336:Drug Antagonism; D005938:Glucocorticoids / Q000494:pharmacology*; D014453:Glucuronosyltransferase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D015735:Mifepristone / Q000494:pharmacology; D008969:Molecular Sequence Data; D000077297:Pregnane X Receptor; D011401:Promoter Regions, Genetic; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors* / Q000235:genetics; D011987:Receptors, Steroid / Q000235:genetics; D014162:Transfection; D000099148:UGT1A1 Enzyme
D005938:Glucocorticoids; D014453:Glucuronosyltransferase; D006854:Hydrocortisone; D015735:Mifepristone; D000077297:Pregnane X Receptor; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D000099148:UGT1A1 Enzyme