title: Mechanical strain-induced c-fos expression in pulmonary epithelial cell line A549.
abstract: Pulmonary epithelial cells are exposed to mechanical strain during physiological breathing and mechanical ventilation. It was not clear which style was more related with cell mechanotransduction. c-fos is known to be a component of a transcription factor, activator protein-1, which is induced by oxidative stress. The regulatory pathways involved in the rapid response of the AP-1 transcription factor, c-fos, to mechanical load in the human pulmonary epithelial cell line A549 were investigated using a four-point bending model. In an effort to better understand what processes are involved in mechanotransduction, we have examined whether and how soon c-fos induction occurs in human A549 shortly after the application of the different mechanical strains stimuli.
authors.affiliation: Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Binwu
authors.initials: B
authors.lastname: Ying
authors.forename: Hong
authors.initials: H
authors.lastname: Fan
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Wen
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.forename: Daishun
authors.initials: D
authors.lastname: Liu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yang
authors.forename: Guo
authors.initials: G
authors.lastname: Chen
authors.forename: Liyang
authors.initials: L
authors.lastname: Dou
authors.forename: Fei
authors.initials: F
authors.lastname: Jiang
mesh_terms: D002460:Cell Line; D004548:Elasticity; D004847:Epithelial Cells / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D010812:Physical Stimulation / Q000379:methods; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000502:physiology*; D013314:Stress, Mechanical
chemical_list: D016760:Proto-Oncogene Proteins c-fos