Neural differentiation of embryonic stem cells induced by conditioned medium from neural stem cell.
Embryonic stem cells can proliferate indefinitely and are capable of differentiating into derivatives of all three embryonic germ layers in vitro, including the neural lineage. The main objective of this study is to test the effects of neural stem cell conditioned medium on the neural differentiation of mouse embryonic stem cells. When cultured in neural stem cell conditioned medium, mouse embryonic stem cells can form floating cell spheres composed of many nestin-positive cells. After trypsinization and growth on gelatin, these embryonic stem cell-derived neural progenitor cells can be expanded for more than 3 months without loss of neural progenitor characteristics. Both neuronal and glial cells can be readily generated from these cells under differentiation conditions. Thus, neural stem cell conditioned medium is a highly potent reagent for inducing the development of mouse embryonic stem cells into the neural lineage, especially neural progenitor cells.
Zhang
Jia-Qing
JQ
Centre for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, Guangzhou, Guangdong, China.
Yu
Xin-Bing
XB
Ma
Bao-Feng
BF
Yu
Wei-Hua
WH
Zhang
Ai-Xia
AX
Huang
Guo
G
Mao
Frank Fuxiang
FF
Zhang
Xiu-Ming
XM
Wang
Zhi-Chong
ZC
Li
Shu-Nong
SN
Lahn
Bruce T
BT
Xiang
Andy Peng
AP
D000818:Animals; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004622:Embryo, Mammalian; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin; D009457:Neuroglia / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
D017077:Culture Media, Conditioned; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D012333:RNA, Messenger