title: Dye staining gap test: an alternative method for assessing marginal gap formation in composite restorations.
abstract: OBJECTIVE
  The aim of this study was to evaluate and compare marginal adaptation of composite restorations assessed by a dye staining method and by scanning electron microscopy (SEM) analysis.
  
  
  METHODS
  Twenty bovine incisors were selected and ground flat to expose dentin. Two cylindrical cavities were prepared on the central area of flattened surfaces. Single bond adhesive system was applied in accordance with the manufacturer's instructions and the cavities were filled with Filtek Z250 or Filtek Flow. The specimens were polished and replicas were obtained in epoxy resin. The replicas were observed by SEM for marginal quality/quantity evaluation. Caries detector was then applied on each specimen for 5 s to verify marginal adaptation through dye staining of the formed gaps on the outer margins. Images of the stained gaps were transferred to a computer measurement program to determine gap length. The length of the gap was expressed as the percentage of total length of the margins observed. Data were submitted to two-way ANOVA and Pearson correlation.
  
  
  RESULTS
  Filtek flow showed 36% and 34% and Filtek Z250 27% and 29% of gap in the margins when evaluated by SEM analysis and by the dye staining test, respectively. There was no difference between the composites, regardless of the evaluation technique. There was a strong positive correlation (r=0.83) between the results obtained with the tested methods to assess marginal gap.
  
  
  CONCLUSIONS
  Dye staining the gaps can be used with good reliability to evaluate the gap formation in composite restorations.
authors.affiliation: Dental Materials Area, Department of Restorative Dentistry, Piracicaba Dental School, UNICAMP, Piracicaba, SP, Brazil. robalonso@yahoo.com
authors.forename: Roberta
authors.initials: R
authors.lastname: Caroline Bruschi Alonso
authors.forename: Gisele
authors.initials: G
authors.lastname: Maria Correr
authors.forename: Leonardo
authors.initials: L
authors.lastname: Gonçalves Cunha
authors.forename: Ana
authors.initials: A
authors.lastname: Flávia Sanches Borges
authors.forename: Regina
authors.initials: R
authors.lastname: Maria Puppin-Rontani
authors.forename: Mário
authors.initials: M
authors.lastname: Alexandre Coelho Sinhoreti
mesh_terms: D000818:Animals; D002417:Cattle; D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry*; D003737:Dental Cavity Preparation; D018772:Dental Marginal Adaptation; D003775:Dental Polishing; D003793:Dental Restoration, Permanent; D003804:Dentin / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D007180:Incisor; D008855:Microscopy, Electron, Scanning; D016628:Replica Techniques; D015203:Reproducibility of Results; D013499:Surface Properties
chemical_list: D004396:Coloring Agents; D003188:Composite Resins; D017220:Dentin-Bonding Agents; C467913:Filtek Flow; C444701:Filtek Z250