title: [Functional properties of prosomal protein 39 kappaDa from Datura stramonium leaves infected with Potato Virus X].
abstract: Free cytoplasmic informosomes isolated from Datura stramonium plants infected by PVX contain a low-molecular ribonucleoprotein complex (RNP). This complex as to its main physico-chemical parameters (sedimentation coefficient 10S, buoyant density in CsSO4 1.31 g/cm3, stability to 1% lauroylsarcosinate-Na) corresponds to the prosome (inhibitory RNP). Prosomes isolated from free mRNP of D. stramonium plants infected by PVX contain the protein of 39 kDa. This protein was shown to be capable to phosphorylate in vitro in the composition of informosomes and prosomes. It is possible that this protein can be the protein-repressor, since it is absent in the translated polysome-associated form of mRNP. The label incorporation has shown that the protein of 39 kDa is able to reduce in vitro the template activity of genomic RNA PVX to 40% and RNA TMIV--to 30%. Moreover, the protein 39 of kDa has the protease activity. It affects substrate-case in like trypsin. It is supposed that it can participate in splitting the intracellular proteins as well as in the expression of the virus genome, it can also influence the template activity of cell RNAs.
authors.forename: L A
authors.initials: LA
authors.lastname: Maksimenko
authors.forename: L F
authors.initials: LF
authors.lastname: Didenko
authors.forename: N I
authors.initials: NI
authors.lastname: Parkhomenko
mesh_terms: D013286:Datura stramonium / Q000378:metabolism / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D008970:Molecular Weight; D018515:Plant Leaves / Q000378:metabolism / Q000821:virology; D017863:Potexvirus / Q000235:genetics* / Q000472:pathogenicity; D046988:Proteasome Endopeptidase Complex / Q000032:analysis* / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics*; D012261:Ribonucleoproteins / Q000032:analysis* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D012367:RNA, Viral; D012261:Ribonucleoproteins; D046988:Proteasome Endopeptidase Complex