[Effect of IL-6 and TNF-alpha on Dengue virus infection of human dendritic cells].
OBJECTIVE
To study the effect of IL-6 and TNF-alpha on Dengue virus (DV) infection of human dendritic cells (DCs).


METHODS
Monocytes isolated from healthy human peripheral blood were incubated in medium with GM-CSF and IL-4 for 7-10 days, and then were collected and identified by transmission electron microscope, immunohistochemistry and lymphocytes stimulatory ability assay. DCs infected with DV type II (DV(2)) were incubated with IL-6, TNF-alpha at high, medium and low concentration. The supernatants were collected at 6 h, 24 h, 48 h, 72 h and 96 h. The virus titers were determined by plaque assay and the number of DCs at 48 h postinfection was mensurated by MTT.


RESULTS
IL-6 at medium or low concentration increased DV(2) replication in DCs, whereas TNF-alpha at high and medium concentration inhibited DV(2) replication in DCs. Cytokines had no effect on the number of normal DCs.


CONCLUSIONS
IL-6 and TNF-alpha may play an important role in the pathogenicity and immunity of Dengue virus.
Shi
Yu-jie
YJ
Department of Microbiology and Immunology, Medical College of Jinan University, Guangzhou 510632, China.
Jiang
Zhen-you
ZY
Zeng
Kang
K
D000818:Animals; D002452:Cell Count; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure / Q000821:virology*; D003715:Dengue; D003716:Dengue Virus / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D046529:Microscopy, Electron, Transmission; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha