Down-regulation of transglutaminase II leads to impaired motility of cancer cells by inactivation of the protein kinase, Akt, and decrease of reactive oxygen species.
We employed RNA interference to determine the role of tissue transglutaminase II (TGase II) in motility of cancer cells. Down-regulation of TGase II by small interfering RNA against TGase II impaired adhesion and motility of HeLa cells by decreasing phosphorylation of the protein kinase, Akt, and reactive oxygen species (ROS). Over-expression of TGase II showed opposite effects. These results suggest potential utility of TGase II for development of therapeutic anti cancer vaccine.
Bae
Jinhee
J
School of Life Sciences, Kangwon National University, Chunchon, 200-701, Korea.
Lee
Yun-Sil
YS
Jeoung
Dooil
D
D019496:Cancer Vaccines; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D015536:Down-Regulation / Q000502:physiology; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D011503:Transglutaminases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D019496:Cancer Vaccines; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D051057:Proto-Oncogene Proteins c-akt; D019204:GTP-Binding Proteins