Proteome analysis of resting human neutrophils.
Neutrophils constitute the first line of host defense against pathogens. In the present study 2-D gel electrophoresis-mass spectrometry technology was employed to analyze the human resting neutrophils proteome. One hundred and two conserved spots were subjected to peptide mass fingerprinting, yielding 22 identifications. Among the identified proteins, nine are related to the inflammatory process, two polypeptides are assigned to metabolic functions and five are classified as structural.
de Souza Castro
Mariana
M
Brazilian Center for Protein Research, Department of Cell Biology, University of Brasília, Brazil.
de Sá
Natan Monsores
NM
Gadelha
Renata Pedroza
RP
de Sousa
Marcelo Valle
MV
Ricart
Carlos André Ornela
CA
Fontes
Belchor
B
Fontes
Wagner
W
D000328:Adult; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000737:chemistry* / Q000378:metabolism; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*
D020543:Proteome