Involvement of wound-induced receptor-like protein kinase in wound signal transduction in tobacco plants.
The wound-induced receptor-like protein kinase (WRK) gene, isolated as one of the genes whose transcripts accumulated during the early period of N gene-dependent synchronized cell death in tobacco mosaic virus-infected tobacco plants, encodes a leucine-rich repeat receptor-like protein kinase, and its transcript was transiently increased 15 min after wounding. In the present study, analysis of a green fluorescent protein fusion protein indicated that WRK is localized in the plasma membrane. In transgenic tobacco plant lines with elevated or suppressed levels of WRK transcript, the wound-induced accumulation of both basic PR-1 and PR-6 transcripts was slightly enhanced or significantly suppressed respectively. The decrease in wound-induced basic PR gene expression in WRK suppressed lines was restored by jasmonic acid (JA) treatment. Furthermore, the levels of wound-induced enzymatic activation of both salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) and wound-induced accumulation of JA were reduced in the WRK suppressed lines in comparison with a control line. These results suggest that WRK functions upstream of SIPK and WIPK and regulates wound signal transduction in tobacco plants.
Takabatake
Reona
R
Plant Physiology Department, National Institute of Agrobiological Sciences, Kannon-dai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan.
Seo
Shigemi
S
Ito
Naoko
N
Gotoh
Yoko
Y
Mitsuhara
Ichiro
I
Ohashi
Yuko
Y
D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000032:analysis; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D054883:Oxylipins; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000032:analysis; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure
D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C011006:jasmonic acid; D017346:Protein Serine-Threonine Kinases; D020928:Mitogen-Activated Protein Kinases; C106405:SA-induced protein kinase; C113423:WIPK protein, Nicotiana tabacum; D020156:Salicylic Acid