Surface plasmon resonance for detection of genetically modified organisms in the food supply.
A review is presented demonstrating that biospecific interaction analysis, using surface plasmon resonance (SPR) and biosensor technologies is a simple, rapid, and automatable approach to detect genetically modified organisms (GMOs). Using SPR, we were able to monitor in real-time the hybridization between oligonucleotide or polymerase chain reaction (PCR)-generated probes and target single-stranded PCR products obtained by using as substrates DNA isolated from normal or transgenic soybean and maize. This procedure allows a one-step, nonradioactive detection of GMOs. PCR-generated probes are far more efficient in detecting GMOs than are oligodeoxyribonucleotide probes. This is expected to be a very important parameter, because information on low percentage of GMOs is of great value. Determination of the ability of SPR-based analysis to quantify GMOs should be considered a major research field for future studies, especially for the analyses of food supplies.
Gambari
Roberto
R
Ferrara University, Biotechnology Center, 44100, Ferrara, Italy. gam@dns.unife.it
Feriotto
Giordana
G
D015374:Biosensing Techniques / Q000379:methods*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D005504:Food Analysis / Q000379:methods*; D005523:Food Supply; D030841:Food, Genetically Modified; D009693:Nucleic Acid Hybridization; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D013025:Glycine max / Q000235:genetics; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors; D003313:Zea mays / Q000235:genetics
D017931:DNA Primers