title: Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp. isolated from the Bay of Bengal.
abstract: Four marine actinobacteria tolerant to 200 g NaCl l(-1) were screened for antibacterial activity against eight patient-derived multiple drug resistant (MDR) bacteria. The active compound (MW 300.2, predicted molecular formula C(20)H(28)O(2)) from an actinobacterium, was inhibitory to three Gram-positive and three Gram-negative MDR bacteria, seven non-clinical Gram-positive, four Gram-negative bacteria and five fungi (MIC: 3.5-4.0 microg ml(-1)). Also, 54% of human leukemia (HL-60) cells were killed by the compound at 0.05 microg ml(-1). Bioreactor production demonstrated unusual primary metabolite kinetics. Molecular phylogenetic analysis showed this typical intertidal inhabitant to be a member of the Streptomyces genus and distinct from other salt-tolerant actinobacteria. As no compound was found to match the properties in several electronic databases, our screening strategy should increase the possibility of discovering bioactive molecules from rare actinobacteria.
authors.affiliation: Environmental Science Programme and Department of Life Science & Biotechnology, Jadavpur University, Kolkata, India.
authors.forename: Malay
authors.initials: M
authors.lastname: Saha
authors.forename: Parasuraman
authors.initials: P
authors.lastname: Jaisankar
authors.forename: Satadal
authors.initials: S
authors.lastname: Das
authors.forename: Kalyan K
authors.initials: KK
authors.lastname: Sarkar
authors.forename: Soma
authors.initials: S
authors.lastname: Roy
authors.forename: Shila E
authors.initials: SE
authors.lastname: Besra
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Vedasiromani
authors.forename: Debashish
authors.initials: D
authors.lastname: Ghosh
authors.forename: Barindra
authors.initials: B
authors.lastname: Sana
authors.forename: Joydeep
authors.initials: J
authors.lastname: Mukherjee
mesh_terms: D039903:Actinobacteria / Q000187:drug effects / Q000378:metabolism*; D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004351:Drug Resistance; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D008969:Molecular Sequence Data; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D012965:Sodium Chloride