title: Molecular nature of sulfhydryl modification by hydrogen peroxide on type 1 ryanodine receptor.
abstract: OBJECTIVE
  To elucidate the molecular nature of sulfhydryl modification by hydrogen peroxide on type 1 ryanodine receptor (RyR1).
  
  
  METHODS
  Rabbit skeletal muscle sarcoplasmic reticulum was treated with hydrogen peroxide, then RyR1 complex was isolated. The proteins in the complex were analysed by electrophoresis, Western blot and electron microscopy.
  
  
  RESULTS
  (1) Hydrogen peroxide induces inter-subunit cross-linking within the tetrameric RyR1 molecule; (2) in parallel to inter-subunit cross-linking, the RyR1 molecule changes morphology; (3) the chemical and morphological changes are reversible: upon reduction by reducing agents, the RyR1 molecule regains its original state.
  
  
  CONCLUSIONS
  These findings suggest that the molecular mechanism of RyR1 channel activity in sarcoplasmic reticulum regulated by hydrogen peroxide is through inter-subunit cross-linking within the tetrameric RyR1 molecule, which in turn induces structural changes of RyR1.
authors.affiliation: Department of Biophysics, Peking University Health Science Centre, Peking University, Beijing 100083, China.
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Han
authors.forename: Ri-sheng
authors.initials: RS
authors.lastname: Wei
authors.forename: F Anthony
authors.initials: FA
authors.lastname: Lai
authors.forename: Anthony F
authors.initials: AF
authors.lastname: Lai
authors.forename: Chang-cheng
authors.initials: CC
authors.lastname: Yin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009124:Muscle Proteins / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology; D011817:Rabbits; D012433:Ryanodine / Q000378:metabolism; D019837:Ryanodine Receptor Calcium Release Channel / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D009124:Muscle Proteins; D016877:Oxidants; D019837:Ryanodine Receptor Calcium Release Channel; D013438:Sulfhydryl Compounds; D012433:Ryanodine; D006861:Hydrogen Peroxide; D002118:Calcium