title: First evidence of the activation of Cg-timp, an immune response component of Pacific oysters, through a damage-associated molecular pattern pathway.
abstract: In a previous work, we characterized a Crassostrea gigas cDNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound healing and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and upstream region. A 1-kb genomic DNA fragment flanking the 5'-end of the gene contains several regulatory elements and notably three NF-kappaB binding sites. The potential involvement of these motifs in Cg-timp gene regulation is discussed.
authors.affiliation: Génome, Populations, Interactions, Adaptation (GPIA), UMR5171 (IFREMER, CNRS, UMII) Université de Montpellier II, place Eugène Bataillon, CC80, 34095 Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Montagnani
authors.forename: J C
authors.initials: JC
authors.lastname: Avarre
authors.forename: J
authors.initials: J
authors.lastname: de Lorgeril
authors.forename: M
authors.initials: M
authors.lastname: Quiquand
authors.forename: V
authors.initials: V
authors.lastname: Boulo
authors.forename: J M
authors.initials: JM
authors.lastname: Escoubas
mesh_terms: D000818:Animals; D001407:Bacillus; D001483:Base Sequence; D049896:Crassostrea / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D015398:Signal Transduction; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015533:Transcriptional Activation; D014733:Vibrio / Q000276:immunology; D014945:Wound Healing / Q000235:genetics / Q000276:immunology*
chemical_list: C431265:TIMP protein, Crassostrea gigas; D019714:Tissue Inhibitor of Metalloproteinases