Strain-stimulated hypertrophy in cardiac myocytes is mediated by reactive oxygen species-dependent Ras S-glutathiolation.
Although reactive oxygen species (ROS) appear to play a central role in mediating myocardial hypertrophy in response to hemodynamic overload, little is known about the molecular targets by which ROS regulate growth signaling. In cardiac myocytes, we tested the hypothesis that mechanical strain causes cellular hypertrophy via ROS-dependent post-translational modification of Ras leading to activation of the Raf/Mek/Erk growth pathway. Cyclic mechanical strain increased Ras activity by 1.5 to 1.6-fold. Adenoviral overexpression of the N17 dominant negative mutant of Ras inhibited strain-stimulated Erk activation and protein synthesis. Strain-stimulated Ras activation was inhibited by overexpression of catalase, indicating that it is redox-dependent. Strain caused S-glutathiolation of Ras, which was inhibited by catalase overexpression and reversed by DTT. MALDI-TOF mass spectrometry demonstrated that in myocytes subjected to strain there was S-glutathiolation of Ras at Cys118. Adenoviral overexpression of a mutated Ras in which Cys118 was substituted with serine inhibited strain-stimulated S-glutathiolation of Ras, Erk activation and protein synthesis. Overexpression of glutaredoxin-1 likewise inhibited strain-stimulated Ras S-glutathiolation, Ras activation, Erk activation and protein synthesis. These findings indicate that mechanical strain causes ROS-dependent S-glutathiolation of Ras at Cys118, leading to myocyte hypertrophy via activation of the Raf/Mek/Erk pathway.
Pimentel
David R
DR
Cardiovascular Medicine Section, Department of Medicine, Boston University Medical Center, Boston, MA 02118, USA.
Adachi
Takeshi
T
Ido
Yasuo
Y
Heibeck
Tyler
T
Jiang
Bingbing
B
Lee
Yong
Y
Melendez
J Andres
JA
Cohen
Richard A
RA
Colucci
Wilson S
WS
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D002312:Cardiomyopathy, Hypertrophic / Q000209:etiology / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D003545:Cysteine / Q000737:chemistry; D048049:Extracellular Signal-Regulated MAP Kinases / Q000096:biosynthesis / Q000378:metabolism; D054477:Glutaredoxins; D005978:Glutathione / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D020935:MAP Kinase Signaling System; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D010088:Oxidoreductases / Q000502:physiology; D051381:Rats; D017382:Reactive Oxygen Species; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013314:Stress, Mechanical; D014162:Transfection; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism*
C516007:Glrx protein, rat; D054477:Glutaredoxins; D017382:Reactive Oxygen Species; D010088:Oxidoreductases; D002374:Catalase; D048049:Extracellular Signal-Regulated MAP Kinases; D018631:ras Proteins; D005978:Glutathione; D003545:Cysteine