Functional expression of the astaxanthin biosynthesis genes from a marine bacterium, Paracoccus haeundaensis.
The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product has been purified to homogeneity. Their molecular characteristics, including enzymatic activities, are reported here.
Lee
Jae Hyung
JH
Department of Microbiology, Pukyong National University, Busan, 608-737, South Korea.
Kim
Young Tae
YT
D002338:Carotenoids / Q000096:biosynthesis / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors / Q000096:biosynthesis; D008967:Molecular Biology / Q000379:methods; D010230:Paracoccus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D024341:Xanthophylls / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D024341:Xanthophylls; D002338:Carotenoids; C005948:astaxanthine