title: Up- and down-regulation of Fragaria x ananassa O-methyltransferase: impacts on furanone and phenylpropanoid metabolism.
abstract: A complex mixture of hundreds of substances determines strawberry (Fragaria x ananassa) aroma, but only approximately 15 volatiles are considered as key flavour compounds. Of these, 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) is regarded as the most important, but it is methylated further by FaOMT (Fragaria x ananassa O-methyltransferase) to 2,5-dimethyl-4-methoxy-3(2H)-furanone (DMMF) during the ripening process. It is shown here that transformation of strawberry with the FaOMT sequence in sense and antisense orientation, under the control of the cauliflower mosaic virus 35S promoter, resulted in a near total loss of DMMF, whereas the levels of the other volatiles remained unchanged. FaOMT repression also affected the ratio of feruloyl 1-O-beta-D-glucose and caffeoyl 1-O-beta-D-glucose, indicating a dual function of the enzyme in planta. Thus, FaOMT is involved in at least two different biochemical pathways in ripe strawberry fruit.
authors.affiliation: Technical University Muenchen, Biomolecular Food Technology, Lise-Meitner-Str. 34, D-85354 Freising, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Lunkenbein
authors.forename: Elma M J
authors.initials: EM
authors.lastname: Salentijn
authors.forename: Heather A
authors.initials: HA
authors.lastname: Coiner
authors.forename: Marjan J
authors.initials: MJ
authors.lastname: Boone
authors.forename: Frans A
authors.initials: FA
authors.lastname: Krens
authors.forename: Wilfried
authors.initials: W
authors.lastname: Schwab
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D002109:Caffeic Acids / Q000378:metabolism; D031985:Fragaria / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005638:Fruit / Q000201:enzymology / Q000378:metabolism*; D005663:Furans / Q000378:metabolism; D015870:Gene Expression; D005960:Glucosides / Q000378:metabolism; D009812:Odorants; D030821:Plants, Genetically Modified / Q000378:metabolism; D011497:Protein O-Methyltransferase / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D002109:Caffeic Acids; D005663:Furans; D005960:Glucosides; D012333:RNA, Messenger; C514186:caffeoyl 1-O-glucose; C514185:feruloyl 1-O-glucose; C514184:4-methoxy-2,5-dimethyl-3(2H)-furanone; C058427:furaneol; D011497:Protein O-Methyltransferase; D015107:4-Butyrolactone