title: A new Leptographium species associated with the northern spruce engraver, Ips perturbatus, in western Canada.
abstract: An undescribed Leptographium species was isolated from the spruce-infesting bark beetle Ips perturbatus collected from felled spruce trees and logs in northern British Columbia and Yukon Territory. Morphologically, this fungus is similar to L. abietinum and L. hughesii but differed in a number of characteristics (e.g. the arrangement of its conidiophores). The fungus grew optimally at 25 C on 2% malt-extract agar and showed a high level of tolerance to cycloheximide. Comparison of rDNA and beta-tubulin gene sequences also confirmed that this Leptographium species represents an undescribed taxon. Thus we described it as a new species, Leptographium fruticetum sp. nov.
authors.affiliation: Department of Wood Science, University of British Columbia, Vancouver, Canada. alamouti@interchange.ubc.ca
authors.forename: Sepideh
authors.initials: S
authors.lastname: Massoumi Alamouti
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Kim
authors.forename: Colette
authors.initials: C
authors.lastname: Breuil
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D001203:Ascomycota / Q000145:classification* / Q000166:cytology / Q000187:drug effects / Q000302:isolation & purification*; D002170:Canada; D003513:Cycloheximide / Q000494:pharmacology; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D025141:Drug Resistance, Fungal; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010781:Photography; D010784:Photomicrography; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013696:Temperature; D014404:Tubulin / Q000235:genetics; D048889:Weevils / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D014404:Tubulin; D003513:Cycloheximide