Low-molecular-weight chromium-binding substance from chicken liver and American alligator liver.
Low-molecular-weight chromium-binding substance (LMWCr), also known as chromodulin, is a chromium-binding oligopeptide proposed to have a function in chromium transport and insulin signaling in mammals. In this work, LMWCr has been isolated and purified for the first time from non-mammalian sources: chicken and American alligator. Milligram quantities of the oligopeptide can be obtained from kilogram quantities of liver. The LMWCr's from both sources are asparatate- and glutamate-rich oligopeptides which possess multinuclear chromium assemblies. The composition and physical and spectroscopic properties of the avian and reptilian LMWCr's are extremely similar to those of their mammalian analogues, suggesting the multinuclear sites of the biomolecule from all three classes of animal possess very similar structures. The chicken and alligator oligopeptides may possess intrinsic phosphotyrosine phosphatase activity.
Hatfield
M Jason
MJ
Department of Chemistry and Coalition for Biomolecular Products, The University of Alabama, Tuscaloosa, AL 35487-0336, USA.
Gillespie
Shanna
S
Chen
Yuan
Y
Li
Zhong
Z
Cassady
Carolyn J
CJ
Vincent
John B
JB
D017667:Adipocytes / Q000201:enzymology; D000489:Alligators and Crocodiles; D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry; D002645:Chickens; D002857:Chromium / Q000378:metabolism*; D018698:Glutamic Acid / Q000737:chemistry; D066298:In Vitro Techniques; D008110:Liver Extracts / Q000737:chemistry*; D008297:Male; D009842:Oligopeptides / Q000302:isolation & purification* / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity
D008110:Liver Extracts; D009842:Oligopeptides; D002857:Chromium; D001224:Aspartic Acid; D018698:Glutamic Acid; D017027:Protein Tyrosine Phosphatases