Characterization of T-DNA insertion mutants and RNAi silenced plants of Arabidopsis thaliana UV-damaged DNA binding protein 2 (AtUV-DDB2).
The human UV-damaged DNA binding protein (UV-DDB), a heterodimeric protein composed of 127 kDa (UV-DDB1) and 48 kDa (UV-DDB2) subunits, has been shown to be involved in DNA repair. To elucidate the in vivo function of plant UV-DDB2, we have analyzed T-DNA insertion mutants of the Arabidopsis thaliana UV-DDB2 subunit (atuv-ddb2 mutants) and AtUV-DDB2 RNAi silenced plants (atuv-ddb2 silenced plants). atuv-ddb2 mutants and atuv-ddb2 silenced plants were both viable, suggesting that AtUV-DDB2 is not essential for survival. Interestingly, both plant types showed a dwarf phenotype, implying impaired growth of the meristem. To the best of our knowledge, this is the first occasion that a dwarf phenotype has been found to be associated with a UV-DDB2 mutation in either plants or animals. The mutants also demonstrated increased sensitivity to UV irradiation, methyl methanesulfonate and hydrogen peroxide treatment, indicating that AtUV-DDB2 is also involved in DNA repair. Our results lead us to suggest that not only does AtUV-DDB2 function in DNA repair, it also has a direct or indirect influence on cell proliferation in the plant meristem.
Koga
Asami
A
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Yamazaki, Noda, Chiba, Japan.
Ishibashi
Toyotaka
T
Kimura
Seisuke
S
Uchiyama
Yukinobu
Y
Sakaguchi
Kengo
K
D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology*; D049109:Cell Proliferation; D004260:DNA Repair / Q000235:genetics; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology*; D005816:Genetic Complementation Test; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018519:Meristem / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D009154:Mutation; D010641:Phenotype; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism
D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; C036483:T-DNA; C513136:UV-DDB2 protein, Arabidopsis; D008741:Methyl Methanesulfonate; D006861:Hydrogen Peroxide