title: Lysophosphatidic receptor, LPA3, is positively and negatively regulated by progesterone and estrogen in the mouse uterus.
abstract: Reciprocal interactions between blastocysts and receptive uteri are essential for successful implantation. This process is regulated by the timely interplay of two ovarian hormones, progesterone and estrogen. However, the molecular targets of these hormones are largely unknown. We showed recently that a small bioactive lysophospholipid, lysophosphatidic acid, plays a pivotal role in the establishment of implantation via its cellular receptor, LPA(3). Here we demonstrate that LPA(3) expression is positively and negatively regulated by steroid hormones in mouse uteri. The LPA(3) mRNA level in the uteri increased during early pseudopregnancy, peaking around 3.5 days post coitus (3.5 d.p.c.), then, decreased to the basal level on 4.5 d.p.c. LPA(3) expression remained at a low level in ovariectomized mice, and administration of progesterone to ovariectomized mice up-regulated LPA(3) mRNA expression. In addition, simultaneous administration of estrogen counteracted the effect of progesterone. These results show that progesterone and estrogen cooperatively regulate LPA(3) expression, thereby contributing to the receptivity of uteri during early pregnancy.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Hama
authors.forename: Junken
authors.initials: J
authors.lastname: Aoki
authors.forename: Koji
authors.initials: K
authors.lastname: Bandoh
authors.forename: Asuka
authors.initials: A
authors.lastname: Inoue
authors.forename: Tomoko
authors.initials: T
authors.lastname: Endo
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Amano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Arai
mesh_terms: D000818:Animals; D015536:Down-Regulation; D004967:Estrogens / Q000494:pharmacology* / Q000502:physiology; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D049368:Receptors, Lysophosphatidic Acid / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015854:Up-Regulation; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D004967:Estrogens; D012333:RNA, Messenger; D049368:Receptors, Lysophosphatidic Acid; D011374:Progesterone