Coded aperture Raman spectroscopy for quantitative measurements of ethanol in a tissue phantom.
Coded aperture spectroscopy allows for sources of large étendue to be efficiently coupled into dispersive spectrometers by replacing the traditional input slit with a patterned mask. We describe a coded aperture spectrometer optimized for Raman spectroscopy of diffuse sources, (e.g., tissue). We provide design details of the Raman system, along with quantitative estimation results for ethanol at non-toxic levels in a lipid tissue phantom. With 60 mW of excitation power at 808 nm, leave-one-out and blind cross-validation of partial least squares (PLS) regression models achieve r(2) > 0.98. Leave-one-out cross-validation demonstrates prediction errors of <15% at the common legal limit for intoxication (17.4 mmol/L = 0.08% by vol) and the best blind cross-validation achieves <12% error at this concentration.
McCain
S T
ST
Department of Electrical and Computer Engineering, and Fitzpatrick Institute for Photonics, Duke University, Durham, NC 27708, USA.
Gehm
M E
ME
Wang
Y
Y
Pitsianis
N P
NP
Brady
D J
DJ
D000465:Algorithms; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D000431:Ethanol / Q000032:analysis* / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D012031:Refractometry / Q000295:instrumentation* / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*
D008051:Lipid Bilayers; D000431:Ethanol