Clinical, histopathological and immunohistochemical findings in a case of megakaryoblastic leukemia in a dog.
The clinical, hematological, and histopathologic features of megakaryoblastic leukemia (M7) were investigated in a 10-year-old female Shih-Tzu dog. Megakaryoblastic leukemia was diagnosed using anti-human platelet glycoprotein (GP IIIa) and anti-human von Willebrand factor (vWF) antibodies. The expression of CD antigen on megakaryoblasts was also assessed using a CD79a monoclonal antibody. Immunological markers allowed visualization of neoplastic megakaryocytes. Antibodies against platelet GP IIIa were demonstrated to be the most useful for the diagnosis of megakaryoblastic leukemia of paraffin-embedded canine tissues. Hematological and histological data coupled with immunohistochemical reactivity for platelet GP IIIa, vWF, and CD79a antigen in blast cells confirmed a diagnosis of M7 megakaryoblastic leukemia.
Park
Hee-Myung
HM
Department of Veterinary Pathology, Small Animal Tumor Diagnostic Center, College of Veterinary Medicine, Konkuk University, Seoul, KwangJin-Ku 143-701, Korea.
Doster
Alan R
AR
Tashbaeva
Roksana E
RE
Lee
Young-Mee
YM
Lyoo
Young S
YS
Lee
Sang-Jin
SJ
Kim
Hee-Jung
HJ
Sur
Jung-Hyang
JH
D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D051925:CD79 Antigens / Q000378:metabolism; D004283:Dog Diseases / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D017809:Fatal Outcome; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D039661:Integrin beta3 / Q000378:metabolism; D007947:Leukemia, Megakaryoblastic, Acute / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D008099:Liver / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D013154:Spleen / Q000473:pathology; D014841:von Willebrand Factor / Q000378:metabolism
D051925:CD79 Antigens; D039661:Integrin beta3; D014841:von Willebrand Factor