Direct detection of 8-oxo-deoxyguanosine using UV resonance Raman spectroscopy.
8-oxo-deoxyguanosine (8-oxo-dG) is a major oxidative lesion in DNA and is responsible for mutation and cancer. Current techniques for detecting 8-oxo-dG are indirect methods. Thus, development of new methodologies is needed to directly detect such oxidative lesions. In this article, we have used ultraviolet resonance Raman (UVRR) spectroscopy as a novel analytical technique for the detection of 8-oxo-dG. Here, the UVRR spectrum of 8-oxo-dG was acquired and compared to that of deoxyguanosine (dG) and deoxyadenosine (dA). Data analysis shows a distinct UVRR spectrum of 8-oxo-dG with characteristic peaks. Detection of 8-oxo-dG was easily achieved from a mixture with dG. These results reveal that UVRR spectroscopy shows promise as a direct method for detecting 8-oxo-dG.
Kundu
Lal Mohan
LM
Department of Chemistry, University of Alberta, Edmonton, AB, Canada.
Loppnow
Glen R
GR
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003839:Deoxyadenosines; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman / Q000379:methods*
D003839:Deoxyadenosines; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine