Theoretical aspects of retention in overloaded columns.
Issues regarding the concentration dependent solute distributions in overloaded chromatographic columns are discussed. Geometric simplicity of wall-coated capillary columns is taken as a reference and the discussion is developed using the example of the absorption isotherm of a solute having more affinity for itself than for the stationary phase. A retention function is deduced solving the equation of motion for the peak maximum, making some approximations. By means of this expression, the applicability of capillary gas chromatography (GC) as a technique for obtaining information on the sorption isotherm is analyzed.
González
Francisco Rex
FR
Química Analítica, Fac. Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, 1900 La Plata, Argentina. rex@quimica.unlp.edu.ar
D002849:Chromatography, Gas / Q000379:methods*; D008962:Models, Theoretical; D012995:Solubility; D013816:Thermodynamics