title: Dietary 5-campestenone (campest-5-en-3-one) enhances fatty acid oxidation in perfused rat liver.
abstract: The effect of dietary 5-campestenone (campest-5-en-3-one), a chemical modification product of a naturally-occurring plant sterol, campesterol, on lipid metabolism was examined using a rat liver perfusion system. Male Sprague-Dawley rats weighing about 140 g were fed a diet supplemented with or without 0.2% 5-campestenone for 14 d. 5-Campestenone feeding resulted in a marked reduction in the concentrations of serum lipids, such as triacylglycerol (TG), cholesterol, phospholipid, and free fatty acid, without influencing food intake or growth. Then, isolated livers from both groups were perfused for 4 h in the presence of an exogenous linoelaidic acid substrate. Dietary 5-campestenone markedly elevated hepatic ketone body production, while cumulative secretions of TG, cholesterol, and phospholipid by the livers of rats fed 5-campestenone were all significantly lowered as compared to those fed without the compound: the extent of the reduction was more prominent in the secretion of TG than other lipid components. In addition, the reduction of TG secretion was concomitantly accompanied by the reduced incorporation of both exogenous and endogenous fatty acids into this lipid molecule. These results suggest that dietary 5-campestenone exerts its hypotriglyceridemic effect, at least, in part through an enhanced metabolism of endogenous and exogenous fatty acids to oxidation at the expense of esterification in rat liver.
authors.affiliation: Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Japan.
authors.forename: Shizuka
authors.initials: S
authors.lastname: Tamaru
authors.forename: Yo
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Masanobu
authors.initials: M
authors.lastname: Sakono
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Fukuda
authors.forename: Ikuo
authors.initials: I
authors.lastname: Ikeda
authors.forename: Rie
authors.initials: R
authors.lastname: Konno
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Kunio
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000821:Animal Feed; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002783:Cholestenones / Q000008:administration & dosage* / Q000494:pharmacology*; D002784:Cholesterol / Q000378:metabolism; D004435:Eating; D005227:Fatty Acids / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D010477:Perfusion; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000378:metabolism
chemical_list: C463760:5-campestenone; D002783:Cholestenones; D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol