[Immunophenotyping of epithelioid-cell granulomas in sarcoidosis].
The specimens of biopsy of the lung and lymph nodes from 24 patients with sarcoidosis were immunophenotyped. The monoclonal antibody panel for immunophenotyping the monocyte-macrophage cells included HLA-DR, CD64, CD163; CD7, CD3, CD4, CD8, HLA-DR, and CD20 were used to assess lymphocytic populations. In sarcoidosis, the majority of cell elements of a granuloma are in a state of activation, as evidenced by HLA-DR expression on the epithelioid, giant cells, T lymphocytes (which was observed in more than in 90% of cases), the extent of this expression decreasing as fibrosis develops. There is a statistically significant correlation between the presence of individual lymphocytic subpopulations and monocyte-macrophage cells in the granuloma. No clear relationship of the phenotype of a granuloma to the clinical form (stage) of sarcoidosis suggests that is based on the common self-sustaining process of formation, functioning, and involution of granulomas, which has no direct agreements in the X-ray and laboratory manifestations of the disease.
Guntupova
L D
LD
Borisov
S E
SE
Typitsyn
N N
NN
Solov'eva
I P
IP
Ots
O N
ON
Latyshev
A N
AN
Semenov
G I
GI
D000328:Adult; D015622:Epithelioid Cells / Q000473:pathology*; D005260:Female; D015769:Granuloma, Respiratory Tract / Q000453:epidemiology / Q000276:immunology* / Q000473:pathology*; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D017565:Sarcoidosis, Pulmonary / Q000453:epidemiology / Q000276:immunology* / Q000473:pathology*
D006684:HLA-DR Antigens