Metagenomic analysis of coastal RNA virus communities.
RNA viruses infect marine organisms from bacteria to whales, but RNA virus communities in the sea remain essentially unknown. Reverse-transcribed whole-genome shotgun sequencing was used to characterize the diversity of uncultivated marine RNA virus assemblages. A diverse assemblage of RNA viruses, including a broad group of marine picorna-like viruses, and distant relatives of viruses infecting arthropods and higher plants were found. Communities were dominated by distinct genotypes with small genome sizes, and we completely assembled the genomes of several hitherto undiscovered viruses. Our results show that the oceans are a reservoir of previously unknown RNA viruses.
Culley
Alexander I
AI
Department of Botany, University of British Columbia, 3529-6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada.
Lang
Andrew S
AS
Suttle
Curtis A
CA
D000818:Animals; D001181:Arthropods / Q000821:virology; D020451:Contig Mapping; D018076:DNA, Complementary; D017753:Ecosystem; D014644:Genetic Variation; D016679:Genome, Viral; D015698:Genomic Library; D023281:Genomics; D005838:Genotype; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification / Q000235:genetics; D010944:Plants / Q000821:virology; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012623:Seawater / Q000821:virology*; D017422:Sequence Analysis, DNA; D026801:Synteny; D019183:Tombusviridae / Q000145:classification / Q000235:genetics
D018076:DNA, Complementary; D012367:RNA, Viral