Multiplex-polymerase chain reaction assay for the authentication of the mackerel Scomber colias in commercial canned products.
A multiplex-polymerase chain reaction (PCR) system was developed for the authentication of the mackerel Scomber colias in commercial canned products. This novel method consists of an S. colias-specific fragment [159 base pairs (bp)] located in the nontranscribed spacer (NTS) sequence, and a Scomber genus-specific PCR product in the 5S rRNA gene (196-201 bp) as a positive amplification control. The system was assayed using 18 different canned products labeled as S. colias. A positive identification was made in all but one sample, revealing this methodology as a potential molecular tool for direct application in the authentication of S. colias canned products.
Infante
Carlos
C
Laboratorio de Identificación de Especies Pesqueras y Acuicolas, CIFPA El Toruño, I.F.A.P.A. C.I.C.E. (Junta de Andalucia), 11500 El Puerto de Santa Maria Cádiz, Spain. carlos.infante.ext@juntadeandalucia.es
Manchado
Manuel
M
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D021903:DNA, Ribosomal Spacer; D005504:Food Analysis / Q000379:methods*; D005519:Food Preservation; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D013045:Species Specificity
D017931:DNA Primers; D021903:DNA, Ribosomal Spacer; D012341:RNA, Ribosomal, 5S