Geroprotection by glycerol: insights to its mechanisms and clinical potentials.
Chaperones, particularly the heat-shock proteins, are considered as key players in the maintenance of protein homeostasis and are associated with longevity and cellular immortalization. In this study, we investigated the geroprotective activity of the chemical chaperone glycerol. Glycerol showed significant chaperoning activity in refolding heat-denatured luciferase in vivo and in protecting cells from heat stress-induced cytotoxicity. This was accompanied by decrease in p53, an upregulation of a stress chaperone mortalin/mtHsp70, and an increase in proteasome activity in the presence of oxidative stress.
Deocaris
Custer C
CC
National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba, Japan.
Shrestha
Bhupal G
BG
Kraft
David C
DC
Yamasaki
Kazuhiko
K
Kaul
Sunil C
SC
Rattan
Suresh I S
SI
Wadhwa
Renu
R
D000375:Aging / Q000187:drug effects* / Q000502:physiology; D005990:Glycerol / Q000494:pharmacology* / Q000627:therapeutic use*; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D008136:Longevity / Q000187:drug effects; D008156:Luciferases / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D020673:Protein Renaturation / Q000187:drug effects
D050883:HSC70 Heat-Shock Proteins; D018832:Molecular Chaperones; D008156:Luciferases; D005990:Glycerol