Hormonal stimulation increases the recruitment of bone marrow-derived myoepithelial cells and periductal fibroblasts into the mammary gland.
Recent reports have revealed that bone marrow (BM)-derived cells can be constituents in a number of organs, especially in remodeling tissue. Using bone marrow transplantation (BMT) technique, we found that BM can serve as a source of both myoepithelial cells and periductal fibroblasts in the mammary gland. The numbers of BM-derived myoepithelial cell were 4.8-fold, and those of periductal fibroblast were 2.4-fold higher in the mice when BMT which was performed at the pubertal stage, as compared with BMT was performed at the postpubertal stage. Treatment with estrogen+progesterone pellet increased numbers of BM-derived myoepithelial cells and periductal fibroblasts, to levels 4.5- and 2.6-fold higher than in placebo mice, respectively. In situ hybridization revealed BM-derived periductal fibroblasts expressed insulin-like growth factor I mRNAs that are known to regulate mammary gland. These results suggest that drastic structural change that is induced by hormonal stimulation increased the recruitment of BM-derived myoepithelial cells and periductal fibroblasts to the mammary gland context.
Sangai
Takafumi
T
Pathology Division, Innovative Medical Research Center, National Cancer Center, 6-5-1 Kashiwanoha, Kashiwa-City, Chiba 277-8577, Japan.
Ishii
Genichiro
G
Fujimoto
Hiroshi
H
Ikehara
Akashi
A
Ito
Takashi
T
Hasebe
Takahiro
T
Magae
Junji
J
Nagashima
Takeshi
T
Miyazaki
Masaru
M
Ochiai
Atsushi
A
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D004338:Drug Combinations; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000166:cytology; D011374:Progesterone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism
D004338:Drug Combinations; D004967:Estrogens; D012333:RNA, Messenger; D011374:Progesterone; D007334:Insulin-Like Growth Factor I