The real-time, three-dimensional analyses of benign and malignant skin tumors by confocal laser scanning microscopy.
BACKGROUND
In obtain images of skin tumors non-invasively with real-time, confocal laser scanning microscope (CLSM) is introduced.


OBJECTIVE
Reconstructed images of given horizontal sections were converted into three-dimensions using the data set of a large number of tomograms in the horizontal directions.


METHODS
To develop the multiplaner reconstruction images of skin tumors in vertical directions and three-dimensionally reconstructed images of tumors will be obtained from the continuously collected horizontal image data sets.


RESULTS
Three-dimensional analyses of the skin tumors from reconstructed images of the CLSM scanning have provided the information as to their physiological characteristics as well as the extent of deep invasion in real-time with non-invasive manner. High performance three-dimensional conversion software was effective in displaying three-dimensional construction of skin tumors.


CONCLUSIONS
The CLSM scanning images followed by three-dimensional reconstruction using them can provide the real-time and non-invasive diagnoses of skin tumors and analyze the radial growth phase of tumors and the three-dimensional growth characteristics.
Ono
Ichiro
I
Sapporo Medical University School of Medicine, Department of Dermatology, Sapporo, Hokkaido 060-8543, Japan. ichiro@sapmed.ac.jp
Sakemoto
Akiko
A
Ogino
Jiro
J
Kamiya
Takafumi
T
Yamashita
Toshiharu
T
Jimbow
Kowichi
K
D000368:Aged; D002280:Basal Cell Carcinoma / Q000473:pathology; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008545:Melanoma / Q000473:pathology; D018613:Microscopy, Confocal; D009508:Nevus, Pigmented / Q000473:pathology; D012871:Skin Diseases / Q000473:pathology*; D012878:Skin Neoplasms / Q000473:pathology*