title: Surface plasmon resonance biosensor for direct detection of antibody against Epstein-Barr virus.
abstract: This paper describes the direct label-free detection of antibodies against the Epstein-Barr virus (anti-EBNA) using a surface plasmon resonance (SPR) biosensor. The antibody detection was performed using the immunoreaction between anti-EBNA and a respective synthetic peptide (EBNA-1), which was conjugated with bovine serum albumin (BSA-EBNA) and immobilized on the sensor surface. Three immobilization chemistries for the attachment of BSA-EBNA were investigated to optimize ligand density and minimize loss of EBNA-1 immunoreactivity. The developed SPR biosensor functionalized with the optimal immobilization method was calibrated and characterized in terms of detection limit, reproducibility, regenerability and storability. It was demonstrated that the sensor is capable of detecting concentrations of anti-EBNA as low as 0.2 ng/ml (approximately 1 pM) both in buffer and 1% human serum and can be stored and regenerated for repeated use.
authors.affiliation: Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, 182 51 Prague, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Vaisocherová
authors.forename: Katerina
authors.initials: K
authors.lastname: Mrkvová
authors.forename: Marek
authors.initials: M
authors.lastname: Piliarik
authors.forename: Pavel
authors.initials: P
authors.lastname: Jinoch
authors.forename: Marie
authors.initials: M
authors.lastname: Steinbachová
authors.forename: Jirí
authors.initials: J
authors.lastname: Homola
mesh_terms: D000906:Antibodies / Q000032:analysis* / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D019309:Epstein-Barr Virus Nuclear Antigens / Q000032:analysis* / Q000276:immunology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004854:Herpesvirus 4, Human / Q000276:immunology*; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods
chemical_list: D000906:Antibodies; D019309:Epstein-Barr Virus Nuclear Antigens; C417029:EBV-encoded nuclear antigen 1