title: One-pot microbial synthesis of 2'-deoxyribonucleoside from glucose, acetaldehyde, and a nucleobase.
abstract: A one-pot enzymatic synthesis of 2'-deoxyribonucleoside from glucose, acetaldehyde, and a nucleobase was established. Glycolysis by baker's yeast (Saccharomyces cerevisiae) generated ATP which was used to produce D: -glyceraldehyde 3-phosphate production from glucose via fructose 1,6-diphosphate. The D: -glyceraldehyde 3-phosphate produced was transformed to 2'-deoxyribonucleoside via 2-deoxyribose 5-phosphate and then 2-deoxyribose 1-phosphate in the presence of acetaldehyde and a nucleobase by deoxyriboaldolase, phosphopentomutase expressed in Escherichia coli, and a commercial nucleoside phosphorylase. About 33 mM 2'-deoxyinosine was produced from 600 mM glucose, 333 mM acetaldehyde and 100 mM adenine in 24 h. 2'-Deoxyinosine was produced from adenine due to the adenosine deaminase activity of E. coli transformants.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Horinouchi
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.forename: Takako
authors.initials: T
authors.lastname: Kawano
authors.forename: Takafumi
authors.initials: T
authors.lastname: Sakai
authors.forename: Kyota
authors.initials: K
authors.lastname: Saito
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Mie
authors.initials: M
authors.lastname: Sasaki
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Mikami
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D003853:Deoxyribonucleosides / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D010430:Pentosyltransferases / Q000494:pharmacology; D010770:Phosphotransferases / Q000378:metabolism; D012267:Ribosemonophosphates / Q000378:metabolism; D012440:Saccharomyces / Q000201:enzymology*; D014169:Transformation, Bacterial / Q000235:genetics* / Q000502:physiology
chemical_list: D003853:Deoxyribonucleosides; D012267:Ribosemonophosphates; C058245:2-deoxyribose 5-phosphate; D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D010770:Phosphotransferases; C022375:phosphopentomutase; D000079:Acetaldehyde; D005947:Glucose