title: Vaccination confers significant protection of sheep against infection with a virulent United Kingdom strain of Corynebacterium pseudotuberculosis.
abstract: Using a virulent United Kingdom Corynebacterium pseudotuberculosis isolate, an ovine experimental model of caseous lymphadenitis was developed, in which the manifestation of disease was equivalent to the naturally observed infection in this country. Subsequently, the capacity of several experimental vaccines to protect against experimental challenge was determined. Sheep were immunised with a recombinant derivative of phospholipase D, deriving from the virulent UK isolate, a formalin-killed bacterin of the same strain, or a bacterin supplemented with recombinant phospholipase D. Following homologous experimental challenge, the phospholipase D and bacterin vaccines were observed to confer statistically significant protection against infection, and appeared to restrict dissemination of challenge bacteria beyond the inoculation site in the majority of animals. More importantly, the combined vaccine succeeded in providing absolute protection against infection, whereby challenge bacteria were eradicated from all vaccinates. In addition to the experimental vaccines, a commercially available CLA vaccine, unlicensed for use in the European Union, was assessed for its capacity to protect against heterologous challenge. The vaccine conferred significant protection, although the dissemination of infection beyond the inoculation site was not restricted as it had been with the previous vaccines. However, no animals immunised with this vaccine manifested infection within the lungs; thus, a potentially important route of disease transmission was eliminated. The results of this study provide information pertinent to the development of an effective caseous lymphadenitis vaccination strategy in the UK.
authors.affiliation: Moredun Research Institute, International Research Centre, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland, United Kingdom. Micheal.Fontaine@Moredun.ac.uk
authors.forename: Michael C
authors.initials: MC
authors.lastname: Fontaine
authors.forename: Graham
authors.initials: G
authors.lastname: Baird
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Connor
authors.forename: Karen
authors.initials: K
authors.lastname: Rudge
authors.forename: Jill
authors.initials: J
authors.lastname: Sales
authors.forename: William
authors.initials: W
authors.lastname: Donachie
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D003354:Corynebacterium Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000276:immunology*; D008199:Lymphadenitis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D008297:Male; D010739:Phospholipase D / Q000008:administration & dosage / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000517:prevention & control*; D006113:United Kingdom; D014611:Vaccination / Q000379:methods / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D001428:Bacterial Vaccines; D014614:Vaccines, Synthetic; D010739:Phospholipase D