Accumulation of recessive lethal mutations in Saccharomyces cerevisiae mlh1 mismatch repair mutants is not associated with gross chromosomal rearrangements.
We examined mismatch repair (MMR)-defective diploid strains of budding yeast grown for approximately 160 generations to determine whether decreases in spore viability due to the uncovering of recessive lethal mutations correlated with an increase in gross chromosomal rearrangements (GCRs). No GCRs were detected despite dramatic decreases in spore viability, suggesting that frameshift and/or other unrepaired DNA replication lesions play a greater role than chromosomal instability in decreasing viability in MMR-defective strains.
Heck
Julie Akiko
JA
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853-2703, USA.
Gresham
David
D
Botstein
David
D
Alani
Eric
E
D048868:Adaptor Proteins, Signal Transducing; D020137:Base Pair Mismatch / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D002869:Chromosome Aberrations; D016368:Frameshift Mutation / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics*; D015321:Gene Rearrangement / Q000502:physiology; D005804:Genes, Lethal / Q000502:physiology; D005808:Genes, Recessive / Q000502:physiology; D008954:Models, Biological; D000070957:MutL Protein Homolog 1; D009154:Mutation / Q000502:physiology*; D009693:Nucleic Acid Hybridization / Q000379:methods; D011336:Probability; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D048868:Adaptor Proteins, Signal Transducing; D005656:Fungal Proteins; C088859:MLH1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000070957:MutL Protein Homolog 1