Neural stem cell separation from the embryonic avian olfactory epithelium by sedimentation field-flow fractionation.
The aim of the present study was to isolate neural stem cells from a complex tissue: the avian olfactory epithelium; by using sedimentation field flow fractionation (SdFFF). By using "Hyperlayer" elution mode, fraction collection and cell characterization methods, results shows that SdFFF could be a useful cell sorter to isolate an enriched, viable and sterile immature neural cell fraction from which the reconstitution of a complete epithelium was possible. In culture, SdFFF eluted cells first led to a "pseudoplacodal" epithelioid cell type from which derived "floating cells". These cells were then able to generate neurosphere-like structures which were composed of cell having many features of immature cells: undifferentiated, self-renewable and multipotentiality. Such a population might be used as a model to improve our understanding of the mechanisms of olfactory neoneurogenesis.
Comte
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I
Laboratoire de Neuro-Immunologie et Développement, EA 3842 Homéostasie Cellulaire & Pathologies, Faculté de Médecine, 2 rue du Dr. Marcland, 87025 Limoges Cedex, France.
Battu
S
S
Mathonnet
M
M
Bessette
B
B
Lalloué
F
F
Cardot
P
P
Ayer-Le Lièvre
C
C
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002642:Chick Embryo; D031521:Fractionation, Field Flow / Q000379:methods*; D020932:Nerve Growth Factor / Q000494:pharmacology; D009831:Olfactory Mucosa / Q000166:cytology / Q000196:embryology*; D020917:Receptor, trkA / Q000096:biosynthesis; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
D020932:Nerve Growth Factor; D020917:Receptor, trkA