The effect of interleukin-17 on hematopoietic cells and cytokine release in mouse spleen.
To evaluate whether the response of hematopoietic cells to interleukin-17 (IL-17) depends on the tissue microenvironment in which hematopoiesis occurs, the influence of recombinant mouse IL-17 on spleen hematopoietic cells and cytokine release was assessed in normal mice in vitro and in vivo. In vitro, IL-17 did not significantly affect the growth of granulocyte-macrophage (CFU-GM) and erythroid (BFU-E and CFU-E) derived colonies. A single injection of IL-17 in vivo exhibited stimulatory effects on hematopoietic cells from both granulocytic and erythroid lineages. The increased number of metamyelocytes 48 h after treatment imply to the IL-17-induced stimulation of granulopoiesis. The number of BFU-E was increased at 24 h, while the number of CFU-E increased 6 h and 24 h after treatment. Since the same treatment in the bone marrow decreased the number of CFU-E, it may be concluded that the local microenvironment plays an important role in IL-17-mediated effects on CFU-E. IL-17 increased the release of IL-6 both in vitro and in vivo, but showed tendency to suppress the constitutive secretion of IL-10 by spleen cells. Our results suggest the complexity of target cell response and interplay of secondary induced cytokines by IL-17 in different hematopoietic organs.
Jovcić
G
G
Institute for Medical Research, Beograd, Serbia. gocaj@imi.bg.ac.yu
Bugarski
D
D
Krstić
A
A
Vlaski
M
M
Petakov
M
M
Mojsilović
S
S
Stojanović
N
N
Milenković
P
P
D000818:Animals; D002470:Cell Survival; D016207:Cytokines / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D020381:Interleukin-17 / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D016207:Cytokines; D020381:Interleukin-17