The QseC sensor kinase: a bacterial adrenergic receptor.
Quorum sensing is a cell-to-cell signaling mechanism in which bacteria respond to hormone-like molecules called autoinducers (AIs). The AI-3 quorum-sensing system is also involved in interkingdom signaling with the eukaryotic hormones epinephrine/norepinephrine. This signaling activates transcription of virulence genes in enterohemorrhagic Escherichia coli O157:H7. However, this signaling system has never been shown to be involved in virulence in vivo, and the bacterial receptor for these signals had not been identified. Here, we show that the QseC sensor kinase is a bacterial receptor for the host epinephrine/norepinephrine and the AI-3 produced by the gastrointestinal microbial flora. We also found that an alpha-adrenergic antagonist can specifically block the QseC response to these signals. Furthermore, we demonstrated that a qseC mutant is attenuated for virulence in a rabbit animal model, underscoring the importance of this signaling system in virulence in vivo. Finally, an in silico search found that the periplasmic sensing domain of QseC is conserved among several bacterial species. Thus, QseC is a bacterial adrenergic receptor that activates virulence genes in response to interkingdom cross-signaling. We anticipate that these studies will be a starting point in understanding bacterial-host hormone signaling at the biochemical level. Given the role that this system plays in bacterial virulence, further characterization of this unique signaling mechanism may be important for developing novel classes of antimicrobials.
Clarke
Marcie B
MB
*Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9048; and.
Hughes
David T
DT
*Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9048; and.
Zhu
Chengru
C
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201.
Boedeker
Edgar C
EC
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201.
Sperandio
Vanessa
V
*Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9048; and vanessa.sperandio@utsouthwestern.edu.
D000818:Animals; D004837:Epinephrine / Q000378:metabolism / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism / Q000494:pharmacology; D010766:Phosphorylation; D011485:Protein Binding; D011817:Rabbits; D011941:Receptors, Adrenergic / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
D029968:Escherichia coli Proteins; C455503:QseB protein, E coli; C455504:QseC protein, E coli; D011941:Receptors, Adrenergic; D009638:Norepinephrine; D004837:Epinephrine