title: Efficacy of HIV PCR techniques to diagnose HIV in infants born to HIV infected mothers--an Indian perspective.
abstract: OBJECTIVE
  To determine the efficiency of HIV DNA PCR (qualitative) and HIV RNA PCR (quantitative) to detect or rule out HIV infection in infants born to HIV infected mothers.
  
  
  METHODS
  Pediatric and perinatal HIV clinic in a tertiary pediatric hospital.
  
  
  METHODS
  Prospective study
  
  
  METHODS
  52 infants born to HIV positive mother were tested for HIV infection by HIV DNA PCR or HIV-1 viral load from 1.5 to 7 months of age. Their HIV status was confirmed by an HIV ELISA test at 18 months of age.
  
  
  RESULTS
  Of the 36 patients tested by HIV DNA PCR test, 15 patients (41.8%) had a positive test and 21 patients (58.2%) had a negative test. 12 patients (80%) had a false positive test (80%) and no patient had a false negative test. Sensitivity of HIV DNA test 100% with specificity of 53.9%. Of the 18 patients tested by HIV viral load, 17 patients (94.4%) had a negative test and 1 patient (5.6%) had a positive test. No patient had a false negative or false positive test. Sensitivity and specificity of HIV viral load was 100%, which was statistically significant (p=0.0004).
  
  
  CONCLUSIONS
  HIV-RNA PCR (quantitative) is highly specific and sensitive test for diagnosing and excluding perinatal HIV infection in children in India.
authors.affiliation: Department of Pediatric HIV Clinic, BJ Wadia Hospital for Children, Parel, Mumbai-400012.
authors.forename: Ira
authors.initials: I
authors.lastname: Shah
mesh_terms: D004279:DNA, Viral / Q000097:blood*; D005069:Evaluation Studies as Topic; D005260:Female; D006679:HIV Seropositivity / Q000175:diagnosis* / Q000635:transmission*; D006801:Humans; D007194:India; D007223:Infant; D018445:Infectious Disease Transmission, Vertical; D009035:Mothers; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood*; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral