Liquid chromatography with tandem mass spectrometry-based proteomic discovery in aging and Alzheimer's disease.
Systems biology offers enormous potential to understand the complexity of human brain aging and neurodegenerative diseases. Proteomics has an important role in these investigations because of its unique strengths and because of the potential central pathogenic contribution of pathological protein to several of these diseases. Here we have reviewed the methods and presented some examples of liquid chromatography-electrospray ionization-tandem mass spectrometry-based proteomics, with and without quantification using isotope-coded affinity tags, in the investigation of aging and Alzheimer's disease. As protocols and methods for improved quantitative high-throughput proteomics constantly improve, this approach will likely continue to provide deeper insight into human brain aging and neurodegenerative diseases.
Montine
Thomas J
TJ
Department of Pathology, University of Washington, Harborview Medical Center, Seattle, Washington 98104, USA. tmontine@u.washington.edu
Woltjer
Randall L
RL
Pan
Catherine
C
Montine
Kathleen S
KS
Zhang
Jing
J
D000375:Aging / Q000378:metabolism*; D000544:Alzheimer Disease / Q000378:metabolism*; D000818:Animals; D002853:Chromatography, Liquid; D006801:Humans; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization