[Ultrastructural organization of carcinoma Lewis (3LL) cells during cisplatin resistance formation].
The electron-microscopic analysis of the morphological status of 3LL (Lewis) carcinoma tumour cells in the process of cisplatin resistant phenotype formation has been performed. It was shown that selection of tumour cells forming cell clones characterized by more complicated nuclear and cytoplasm organization took place. The tumour cells had the diffused nuclear chromatin; nuclear envelope had the numerous pores with expanded diaphragms. The prominent nucleoli consisted of the active centres surrounded by considerable areas of the condensed nucleolar chromatin. Cell cytoplasm contained the well-developed Goldgi complex and the numerous well-structured myelinoid formations in the form of dense-wrapped concentric membrane structures. The obtained data can morphologically confirm the hypothesis of Gately D.P. and Howel S.B., 1993, thain the process of resistant phenotype formation the tumour cells can create the cellular mechanisms to remove the drug from the cell and to correct the damages of the cellular nucleus and cytoplasm.
Tregubova
N A
NA
Iurchenko
O V
OV
Todor
I N
IN
Sudiakov
V S
VS
Kulik
G I
GI
Chekhun
V F
VF
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy* / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002945:Cisplatin / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D019008:Drug Resistance, Neoplasm; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron
D002945:Cisplatin