title: Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane.
abstract: Escherichia coli bolA is a morphogene involved in stress response and cell division. Overexpression of bolA induces biofilm formation and affects the levels of carboxypeptidases PBP5, PBP6 and beta-lactamase AmpC. In this study, we have shown that changes in the expression of bolA result in alterations in the properties of the outer membrane. The sensitivity to detergents and vancomycin was reduced when bolA was overexpressed and fluorescent probes indicated that different levels of bolA had an effect on outer membrane protein accessibility. Moreover, bolA was shown to be involved in the modulation of the OmpF/OmpC balance.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
authors.forename: Patrick
authors.initials: P
authors.lastname: Freire
authors.forename: Helena L A
authors.initials: HL
authors.lastname: Vieira
authors.forename: Ana R
authors.initials: AR
authors.lastname: Furtado
authors.forename: Miguel A
authors.initials: MA
authors.lastname: de Pedro
authors.forename: Cecília M
authors.initials: CM
authors.lastname: Arraiano
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002463:Cell Membrane Permeability; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial / Q000502:physiology*; D018272:Porins / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; C113724:OmpC protein; C109237:OmpF protein; D018272:Porins