Construction of a bacterial artificial chromosome library and characterization of hrp/hrc gene cluster of Pseudomonas syringae pathovar tagetis LMG5090.
Pseudomonas syringae pv. tagetis causes apical chlorosis of several plant species in the Asteraceae, including marigold. As a means to facilitate the isolation of pathogenicity genes and to characterize the genome of this bacterium, we have constructed a bacterial artificial chromosome library of P. syringae pv. tagetis strain LMG5090. The library consists of 1,536 clones with insert size ranging from 30 to 160 kb and an average size of 86 kb. Based upon colony hybridization, the BAC clone 420E23 containing the hrp/hrc gene cluster encoding the type III secretion system was identified from this library and subsequently shotgun sequenced. The hrp/hrc gene cluster of P. syringae pv. tagetis has a 23 kb sequence which contains 27 open reading frames. Comparative analysis of the hrp/hrc gene cluster of P. syringae pv. tagetis LMG5090, P. syringae pv. tomato DC3000, P. syringae pv. syringae B728a, and P. syringae pv. phaseolicola 1448A revealed that the entire hrp/hrc gene cluster of P. syringae pv. tagetis is conserved and identically arranged in all four pathovars.
Song
Eun-Sung
ES
National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea.
Park
Young-Jin
YJ
Chae
Soo-Cheon
SC
Kim
Jeong-Gu
JG
Cho
Hee-Jung
HJ
Lee
Gil-Bok
GB
Lee
Byoung-Moo
BM
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D017124:Conserved Sequence / Q000235:genetics*; D015723:Gene Library; D005818:Genetic Engineering / Q000379:methods; D005810:Multigene Family / Q000235:genetics*; D044224:Pseudomonas syringae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity
D001425:Bacterial Outer Membrane Proteins; C075462:harpin protein, Erwinia amylovora