Microcystin-RR-induced apoptosis in tobacco BY-2 cells.
Our previous studies showed that microcystin-RR could induce oxidative damage in plant cells as they do with animal cells. However, whether microcystin can induce plant cell apoptosis is still unknown. In this study, the morphological changes of tobacco BY-2 suspension cells exposed to microcystin-RR were observed under light microscopy and transmission electron microscopy, and apoptosis was clearly distinguished by intense perinuclear chromatin margination, condensation of nuclear chromatin after 6d exposure of 50mg/L (about 50microM) microcystin-RR. We also found that microcystin-RR can induce tobacco cell apoptosis in a dose- and time-dependent manner with flow cytometry analysis. Our study provides the first evidence that microcystins can induce plant cell apoptosis.
Yin
Liyan
L
Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan 430074, China.
Huang
Jiaquan
J
Li
Wei
W
Liu
Yongding
Y
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure; D000458:Cyanobacteria / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D008387:Marine Toxins; D052998:Microcystins; D046529:Microscopy, Electron, Transmission; D010456:Peptides, Cyclic / Q000633:toxicity*; D014026:Nicotiana / Q000187:drug effects* / Q000648:ultrastructure
D002843:Chromatin; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C063855:microcystin RR