Quantitative comparison of the signals of an electrochemical bioactivity sensor during the cultivation of different microorganisms.
The microbial activity of different microorganisms was determined by means of an electrochemical bioactivity sensor (BAS). The BAS is based on a biofuel cell and was used for analytical purposes. Online determination of microbial activity using the BAS demonstrated that when different microorganisms with different metabolic pathways were cultivated, a distinct activity signal was detectable with all organisms applied. Furthermore, the results permitted a quantitative comparison of the BAS signals. Among other findings it was shown that the quotient of the BAS signal and the utilized glucose varied from 0.16-29.08 mV g(-1), the quotient of the maximum BAS signal and the released energy of the reaction exhibited a lower variation of 0.07-0.19 mV kJ(-1). Furthermore it was demonstrated that the highest BAS signals could be measured during anaerobic E. coli fermentations, the reason being the formation of electroactive fermentation products, such as formic acid and H(2).
Holtmann
Dirk
D
Karl-Winnacker-Institut der DECHEMA eV, Theodor-Heuss-Allee 25, 60486, Frankfurt am Main, Germany.
Schrader
Jens
J
Sell
Dieter
D
D001420:Bacteria, Aerobic / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology; D015374:Biosensing Techniques / Q000295:instrumentation*; D004926:Escherichia coli / Q000378:metabolism; D005285:Fermentation / Q000502:physiology*; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000378:metabolism