A novel in vitro cultivation system to produce and isolate soluble factors released from hyphae of arbuscular mycorrhizal fungi.
The Petri plate dual in vitro culture system for arbuscular mycorrhizal fungi (AMF) is ill-suited for the production and isolation of extraradically released soluble factors, thereby preventing the further characterization of such compounds. To overcome this technical bottleneck, we here describe a novel cultivation system using the standard AMF Glomus intraradices-carrot dual culture as a model. This new system is capable of producing soluble factors in copious amounts without any interference from host roots. The greatest advantages of this culture method include ease of handling and reusability of the culture vessel, thus making it a very cost effective system.
Gadkar
Vijay
V
Division of Biological Sciences, University of Montana, Missoula, MT 59812-0004, USA. vijay.gadkar@mso.umt.edu
Driver
James D
JD
Rillig
Matthias C
MC
D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D018552:Daucus carota / Q000737:chemistry / Q000382:microbiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D025301:Hyphae / Q000378:metabolism*; D038821:Mycorrhizae / Q000737:chemistry / Q000378:metabolism*; D012995:Solubility
D005656:Fungal Proteins