The analysis of proteome changes in sunflower seeds induced by N+ implantation.
In this work, the proteomic changes induced by N+ ion implantation were investigated using a sunflower seed model by a two-dimensional electrophoretic analysis. To further understand the changes of total protein irradiated with N+ ion, a proteomic analysis of N+ ion implantation seeds was developed. Among approximately 369 total protein spots displayed in 2-D gels, eight specific proteins were found in non-implanted seeds while four proteins were found in implanted seeds. Six proteins were used for MALDI-TOF MS analysis, of which only two had been reported before. The proteins designated as No.29 showed 23.4% homology to MADS-box transcriptional factor HAM59, while No. 279 protein had 23.20% identity to homeobox-leucine zipper protein HAHB-4. The analysis of proteome changes induced by N+ implantation could provide a new clue to studying mutation mechanism of ion implantation. To our knowledge, this is the first report about the analysis of proteome changes induced by N+ implantation in sunflower seeds.
Guijun
Dong
D
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.
Weidong
Pan
P
Gongshe
Liu
L
D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D006368:Helianthus / Q000033:anatomy & histology / Q000502:physiology* / Q000528:radiation effects; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D012639:Seeds / Q000737:chemistry* / Q000528:radiation effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010940:Plant Proteins; D020543:Proteome; D009584:Nitrogen