[Effect of photodynamic therapy with ATX-S 10 (Na) on experimental choroidal neovascularization].
OBJECTIVE
To evaluate an appropriate irradiative condition for selective occlusion of experimental choroidal neovascularization(CNV) with photodynamic therapy (PDT) using ATX-S 10 (Na).


METHODS
Experimental CNV was induced in monkey eyes by laser photocoagulation. PDT(dose of irradiative energy 40 to 80J/cm2) was performed after 3.5 mg/kg of body weight intravenous injections of ATX-S 10(Na). CNV and retinal vessel occlusion induced by PDT was evaluated by fluorescein angiography (FA) at 1 and 7 days after irradiation. If FA showed no fluorescein dye leakage from CNV at 1 and 7 days after irradiation, CNV was evaluated by histopathological analysis at 7 days after irradiation.


RESULTS
Within 30 to 33 minutes after ATX-S 10(Na) injection and irradiation with 50 to 60 J/ cm2, FA showed no fluorescein dye leakage from CNV and no closure of retinal vessels at 1 and 7 days after irradiation. Light micrographs showed occluded CNV, and retinal vessels remained patent and there was no apparent change in the inner layer of the retina.


CONCLUSIONS
Irradiative condition of ATX-S10 (Na) 3.5 mg/kg was appropriate 30 to 33 minutes after ATX-S 10(Na) injection and irradiation with 50 to 60 J/cm2.
Hirose
Tokihito
T
Department of Ophthalmology, Surugadai Hospital, Nihon University. 1-8-13 Kanda, Surugadai, Chiyoda-ku, Tokyo 101-8309, Japan. tokihito@med.nihon-u.ac.jp
Nakajima
Masami
M
Kyou
Tetsuhiro
T
Yuzawa
Mitsuko
M
D000818:Animals; D020256:Choroidal Neovascularization / Q000188:drug therapy*; D008252:Macaca fascicularis; D010778:Photochemotherapy / Q000379:methods*; D011166:Porphyrins / Q000627:therapeutic use*
C117772:ATX S10Na(II); D011166:Porphyrins