title: Synthesis of mixed sequence borane phosphonate DNA.
abstract: A novel solid-phase phosphoramidite-based method has been developed for the synthesis of borane phosphonate DNA. Keys to this new approach are replacement of the common 5'-dimethoxytrityl blocking group with a 5'-silyl ether and the use of new protecting groups on the bases (adenine, N6-dimethoxytrityl; cytosine, N4-trimethoxytrityl; guanine, N2-[9-fluorenylmethoxycarbonyl]; thymine, N3-anisoyl). Because of these developments, it is now possible for the first time to synthesize oligodeoxynucleotides having any combination of the four 2'-deoxynucleosides and both phosphate and borane phosphonate internucleotide linkages (including oligomers having exclusively borane phosphonate linkages).
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.
authors.forename: Heather Brummel
authors.initials: HB
authors.lastname: McCuen
authors.forename: Mary S
authors.initials: MS
authors.lastname: Noé
authors.forename: Agnieszka B
authors.initials: AB
authors.lastname: Sierzchala
authors.forename: Adrian P
authors.initials: AP
authors.lastname: Higson
authors.forename: Marvin H
authors.initials: MH
authors.lastname: Caruthers
mesh_terms: D001880:Boranes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry*; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D063065:Organophosphonates / Q000737:chemistry*
chemical_list: D001880:Boranes; D009841:Oligonucleotides; D063065:Organophosphonates; D004247:DNA