Complement activation by sulfonated poly(ethylene glycol)-acrylate copolymers through alternative pathway.
Previously, novel poly(ethylene glycol) (PEG) and sulfonated PEG acrylate (PEG-SO(3)A/OA) copolymers were prepared as coating and/or blending materials for biomedical applications. Surfaces modified with copolymers exhibited increased anti-coagulation properties and decreased plasma adsorption level due to increased hydrophilic properties and reorientation characteristics of PEG/PEG-SO(3)A chains in water phase. As continuation study, anti-complement effects of PEG-SO(3)/OA copolymers were investigated in vitro, and compared with those of low-density polyethylene (LDPE) and PEG/OA. C3 activation by PEG-SO(3)/OA samples was lower than that by PEG/OA samples, which was attributed to decreased surface nucleophile level of samples. PEG-SO(3)/OA samples increased inhibition of Bb production, resulting in decreased C5 activation. Owing to reduced activations of C3 and C5, PEG-SO(3)/OA samples markedly decreased SC5b-9 levels in plasma.
Jang
Hong Seok
HS
Department of Therapeutic Radiology, Uijeongbu St. Mary's Hospital, College of Medicine, Catholic University, Uijeongbu 480-130, Korea.
Ryu
Kyu Eun
KE
Ahn
Woong Shick
WS
Chun
Heung Jae
HJ
Dal Park
Hyung
H
Park
Ki Dong
KD
Kim
Young Ha
YH
D000179:Acrylates / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible; D015936:Complement C5a / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D003170:Complement Pathway, Alternative / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*
D000179:Acrylates; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D015938:Complement Membrane Attack Complex; D011092:Polyethylene Glycols; D015936:Complement C5a