title: Pursuing gene regulation 'logic' via RNA interference and chromatin immunoprecipitation.
abstract: RNA interference and chromatin immunoprecipitation are now firmly established as useful methods for studying mechanisms of gene regulation in vivo. Their combined use can help elucidate gene regulation 'logic' by aiding in target gene identification for transcription factors and chromatin-modifying complexes.
authors.affiliation: Howard Hughes Medical Institute and Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-1662, USA.
authors.forename: Caiyi C
authors.initials: CC
authors.lastname: Li
authors.forename: Vladimir R
authors.initials: VR
authors.lastname: Ramirez-Carrozzi
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Smale
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000302:isolation & purification; D047369:Chromatin Immunoprecipitation; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology; D004265:DNA Helicases / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D008070:Lipopolysaccharides; D035683:MicroRNAs; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; C499503:Smarca2 protein, mouse; D014157:Transcription Factors; C084108:SMARCA4 protein, human; C513194:Smarca4 protein, mouse; D004265:DNA Helicases