title: Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry.
abstract: A comparative analysis of the protein composition of Acidithiobacillus ferrooxidans cells grown on elemental sulfur and ferrous iron was performed. A newly developed protocol involving immobilized pH gradients, improved protein reduction, mass spectrometry protein identification and full genome sequence information was applied. This approach resulted in more than 1300 protein spots displayed in broad and basic pH ranges, the best A. ferrooxidans proteome resolution to date. A comparative image analysis revealed that the proteome was significantly influenced by the growth type, and allowed for the detection of many physiologically important proteins. Among them were sulfate adenylyltransferase and sulfide dehydrogenase, which are involved in sulfate assimilation and sulfide metabolism, respectively. Many other proteins were related to important processes like cell attachment and electron transport. Co-migration of phosphate and sulfate transport proteins was also observed.
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Bouchal
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Zdráhal
authors.forename: Sárka
authors.initials: S
authors.lastname: Helánová
authors.forename: Oldrich
authors.initials: O
authors.lastname: Janiczek
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Hallberg
authors.forename: Martin
authors.initials: M
authors.lastname: Mandl
mesh_terms: D042763:Acidithiobacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D010084:Oxidation-Reduction; D040901:Proteomics / Q000379:methods*; D013430:Sulfate Adenylyltransferase / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013455:Sulfur; D007501:Iron; D013430:Sulfate Adenylyltransferase