title: Spectrophotometric determination of acyclovir and ribavirin in their dosage forms.
abstract: Two simple, accurate, and reliable spectrophotometric methods have been developed for the determination of 2 antiviral drugs, acyclovir (ACV) and ribavirin (RBV), in their pharmaceutical formulations. These methods are based on oxidation of the 2 drugs with either cerium (IV) ammonium sulfate (Method A) or potassium persulfate (Method B). The products of oxidation in both methods are coupled with 3-methylbenzothiazolin 2-one hydrazone, producing a deep blue color with a maximum absorption wavelength at 630 nm. In Method A, the absorbance-concentration plots were linear over the ranges of 5-50 and 10-60 microg/mL with detection limits of 0.18 microg/mL (8 x 10(-7) M) and 0.63 microg/mL (2.58 x 10(-6) M) for ACV and RBV, respectively. In Method B, the ranges were 5-45 and 20-50 microg/mL with detection limits of 0.11 microg/mL (4.88 x 10(-7) M) and 1.40 microg/mL (5.73 x 10(-6) M) for the 2 drugs, respectively. The molar absorptivities were 4.1 x 10(3) and 3.65 x 10(3) L/mol/cm in Method A and 5.03 x 10(3) and 3.97 x 10(3) L/mol/cm in Method B for the 2 drugs, respectively. The proposed methods were applied successfully for the determination of the 2 drugs in their pharmaceutical formulations. The percentage recoveries +/- standard deviation were 99.57 +/- 0.86 and 100.82 +/- 0.46 for ACV; 99.41 +/- 1.08 and 100.35 +/- 1.03 for RBV. The results obtained were compared statistically with those given by official methods and showed no significant differences regarding accuracy and precision.
authors.affiliation: University of Mansoura, Faculty of Pharmacy, Department of Analytical Chemistry, Mansoura, 35516, Egypt.
authors.forename: Mohie Khaled Sharaf
authors.initials: MK
authors.lastname: El-Din
authors.forename: Amina Mohammed
authors.initials: AM
authors.lastname: El-Brashy
authors.forename: Zeinab Awad
authors.initials: ZA
authors.lastname: Sheribah
authors.forename: Rania Mohammed
authors.initials: RM
authors.lastname: El-Gamal
mesh_terms: D000212:Acyclovir / Q000032:analysis* / Q000302:isolation & purification; D000645:Ammonium Sulfate / Q000494:pharmacology; D000998:Antiviral Agents / Q000032:analysis* / Q000302:isolation & purification; D002563:Cerium / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004304:Dosage Forms; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D017680:Potassium Compounds / Q000494:pharmacology; D015203:Reproducibility of Results; D012254:Ribavirin / Q000032:analysis* / Q000302:isolation & purification; D013053:Spectrophotometry / Q000379:methods*; D013431:Sulfates / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000998:Antiviral Agents; D004304:Dosage Forms; D017680:Potassium Compounds; D013431:Sulfates; D002563:Cerium; D012254:Ribavirin; C009007:potassium persulfate; D010100:Oxygen; D000645:Ammonium Sulfate; D000212:Acyclovir