A low temperature bonding of quartz microfluidic chip for serum lipoproteins analysis.
A low-temperature bonding method for microfabrication of quartz microfluidic chips has been developed. The bonding process involved two steps: pre-bonding and post-annealing at low temperature. The bonding quality was evaluated by measuring the shear force at bonding interface and the electrical properties of the chips. Shear force of 5.66 MPa (566 N/cm(2)) was obtained in a bonded chip after a post-annealing at 200 degrees C for 6 h. We owe the strong bonding strength to the formation of Si-O-Si bonds at the bonding interface during the post-annealing stage. The bonding procedures were not sensitive to surrounding and could be performed in a routine laboratory without clean room conditions. The performance of the fabricated microfluidic chips was tested by capillary zone electrophoresis (CZE) of serum lipoproteins with laser-induced fluorescence (LIF). The low-density (LDL) and high-density (HDL) lipoproteins in the serum was separated completely by using tricine buffer with methylglucamine.
Zhuang
Guisheng
G
Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, 865 Chang Ning Road, 200050 Shanghai, China. guishengzhuang@gmail.com
Jin
Qinghui
Q
Liu
Jing
J
Cong
Hui
H
Liu
Kangdong
K
Zhao
Jianlong
J
Yang
Mengsu
M
Wang
Huimin
H
D003080:Cold Temperature; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D011791:Quartz / Q000737:chemistry
D008074:Lipoproteins; D011791:Quartz