title: Regulation of calmodulin-stimulated cyclic nucleotide phosphodiesterase (PDE1): review.
abstract: The response of living cells to change in cell environment depends on the action of second messenger molecules. The two second messenger molecules cAMP and Ca2+ regulate a large number of eukaryotic cellular events. Calmodulin-stimulated cyclic nucleotide phosphodiesterase (PDE1) is one of the key enzymes involved in the complex interaction between cAMP and Ca2+ second messenger systems. Some PDE1 isozymes have similar kinetic and immunological properties but are differentially regulated by Ca2+ and calmodulin. Accumulating evidence suggests that the activity of PDE1 is selectively regulated by cross-talk between Ca2+ and cAMP signalling pathways. These isozymes are also further distinguished by various pharmacological agents. We have demonstrated a potentially novel regulation of PDE1 by calpain. This study suggests that limited proteolysis by calpain could be an alternative mechanism for the activation of PDE1. We have also shown PDE1 activity, expression and effect of calpain in the rat model in vitro of cardiac ischemia-reperfusion.
authors.affiliation: Department of Pathology and Laboratory Medicine, College of Medicine, University of Saskatchewan, Saskatoon SK S7N 4H4, Canada. rsharma@scf.sk.ca
authors.forename: Rajendra K
authors.initials: RK
authors.lastname: Sharma
authors.forename: Shankar B
authors.initials: SB
authors.lastname: Das
authors.forename: Ashakumary
authors.initials: A
authors.lastname: Lakshmikuttyamma
authors.forename: Ponniah
authors.initials: P
authors.lastname: Selvakumar
authors.forename: Anuraag
authors.initials: A
authors.lastname: Shrivastav
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D008954:Models, Biological; D051381:Rats
chemical_list: D002147:Calmodulin; D007527:Isoenzymes; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D002118:Calcium