Involvement of Y-family DNA polymerases in mutagenesis caused by oxidized nucleotides in Escherichia coli.
Escherichia coli DNA polymerase IV incorporated 2-hydroxy-dATP opposite template guanine or thymine and 8-hydroxy-dGTP exclusively opposite adenine in vitro. Mutator phenotypes in sod/fur strains were substantially diminished by deletion of dinB and/or umuDC. DNA polymerases IV and V may be involved in mutagenesis caused by incorporation of the oxidized deoxynucleoside triphosphates.
Yamada
Masami
M
Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Nunoshiba
Tatsuo
T
Shimizu
Masatomi
M
Gruz
Petr
P
Kamiya
Hiroyuki
H
Harashima
Hideyoshi
H
Nohmi
Takehiko
T
D019951:DNA Polymerase beta / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D016296:Mutagenesis; D009711:Nucleotides / Q000378:metabolism*; D010084:Oxidation-Reduction
D009711:Nucleotides; D019951:DNA Polymerase beta