Effects of ephedrae herba on melanogenesis and gene expression profiles using cDNA microarray in B16 melanocytes.
Ephedrae Herba (EH) is obtained by drying the stems of Ephedra sinica Stapf (family Ephedraceae). EH has been used clinically to treat colds and to reduce edema in skin. The effects of EH on melanogenesis were studied in B16 murine melanocytes. The tyrosinase activity and melanin content were measured after incubation with EH. Both reverse transcription-polymerase chain reaction and cDNA microarray analysis were used to study the mechanism of EH action. EH decreased tyrosinase activity and melanin content in a dose-dependent manner. EH decreased microphthalmia-associated transcription factor (MITF) and tyrosinase gene expression. These effects were controlled by MITF-mediated regulation of tyrosinase gene expression. EH also altered the expression of about 100 other genes. These results suggest that EH may be used clinically to treat freckles and liver spots.
Kim
Hyeong Jun
HJ
College of Oriental Medicine, Semyung University, Jechon, South Korea.
Cho
Yeong Do
YD
Leem
Kang-Hyun
KH
Lee
Dong-Nyung
DN
Kim
Ee-Hwa
EH
Kim
Myung-Gyou
MG
Kim
Dae-Keun
DK
Shin
Tae-Yong
TY
Boo
Yungmin
Y
Lee
Je-Hyun
JH
Kim
Hye Kyung
HK
D000818:Animals; D045744:Cell Line, Tumor; D029790:Ephedra sinica; D015870:Gene Expression / Q000187:drug effects; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects*; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D010936:Plant Extracts; D014442:Monophenol Monooxygenase