title: [Large-scale screening of mtDNA A1555G mutation in China and its significance in prevention of aminoglycoside antibiotic induced deafness].
abstract: OBJECTIVE
  To explore the necessity of large-scale screening of mtDNA A1555G mutation in prevention of aminoglycoside antibiotic induced deafness (AAID) and to develop a feasible method to prevent AAID.
  
  
  METHODS
  A total of 1836 patients with non-syndromic hearing impairment (NSHI), 1352 students of schools for deaf-mutes in 11 provinces and municipality in China, 413 out-patients, and 71 persons from the families with maternal relatives suffering from AAID, underwent questionnaire survey and/or PCR for A-to-G mutation at nucleotide 1555 of the mitochondrial genome.
  
  
  RESULTS
  Sixty three patients with mtDNA A1555G mutation were found among the 1836 NSHI patients. Fifty-two maternal pedigrees were identified. 536 cases with normal hearing from these pedigrees were informed to avoid using aminoglycoside antibiotics (AmAn).
  
  
  CONCLUSIONS
  Large-scale screening of mtDNA A1555G mutation and relevant health education to avoid use of AmAn are effective to prevent ototoxicity in the A1555G carriers and their maternal relatives.
authors.affiliation: Department of Otolaryngology, General Hospital of People's Liberation Army, Beijing 100853, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Pu
authors.initials: P
authors.lastname: Dai
authors.forename: De-liang
authors.initials: DL
authors.lastname: Huang
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Yuan
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Li
authors.forename: Ju-yang
authors.initials: JY
authors.lastname: Cao
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.forename: Rui-ning
authors.initials: RN
authors.lastname: Zhang
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Lin
authors.forename: Xiu-hui
authors.initials: XH
authors.lastname: Zhu
authors.forename: Yong
authors.initials: Y
authors.lastname: He
authors.forename: You-jun
authors.initials: YJ
authors.lastname: Yu
authors.forename: Kun
authors.initials: K
authors.lastname: Yao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000617:Aminoglycosides / Q000009:adverse effects; D000900:Anti-Bacterial Agents / Q000009:adverse effects; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004272:DNA, Mitochondrial / Q000235:genetics*; D003638:Deafness / Q000139:chemically induced* / Q000453:epidemiology / Q000517:prevention & control*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D009154:Mutation; D010375:Pedigree; D013334:Students; D011795:Surveys and Questionnaires
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004272:DNA, Mitochondrial