On the statistical analysis of the GS-NS0 cell proteome: imputation, clustering and variability testing.
We have undertaken two-dimensional gel electrophoresis proteomic profiling on a series of cell lines with different recombinant antibody production rates. Due to the nature of gel-based experiments not all protein spots are detected across all samples in an experiment, and hence datasets are invariably incomplete. New approaches are therefore required for the analysis of such graduated datasets. We approached this problem in two ways. Firstly, we applied a missing value imputation technique to calculate missing data points. Secondly, we combined a singular value decomposition based hierarchical clustering with the expression variability test to identify protein spots whose expression correlates with increased antibody production. The results have shown that while imputation of missing data was a useful method to improve the statistical analysis of such data sets, this was of limited use in differentiating between the samples investigated, and highlighted a small number of candidate proteins for further investigation.
Ahmad
Norhaiza
N
Institute of Mathematics and Statistics, University of Kent, Canterbury, Kent, UK.
Zhang
Jian
J
Brown
Phillip J
PJ
James
David C
DC
Birch
John R
JR
Racher
Andrew J
AJ
Smales
C Mark
CM
D000465:Algorithms; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D025341:Principal Component Analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods / Q000706:statistics & numerical data*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D000911:Antibodies, Monoclonal; D020543:Proteome; D011994:Recombinant Proteins; D005974:Glutamate-Ammonia Ligase