title: DNA test to sex the lesser rhea (Rhea pennata pennata).
abstract: 1. A polymerase-chain-reaction (PCR)-based test was adapted to sex the lesser rhea. Feathers instead of blood were used as the source of DNA. 2. The primers used allowed the assignment of sex by amplification of Z and W chromosome linked sequences. 3. This method proved to be safe, accurate and non-invasive. 4. The W chromosome linked fragment was sequenced and compared to other ratite sequences.
authors.affiliation: Centro de Zoología Aplicada, Facultad de Ciencias Exactas Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: H J
authors.initials: HJ
authors.lastname: Rossi Fraire
authors.forename: M B
authors.initials: MB
authors.lastname: Martella
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis*; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D020311:Rheiformes / Q000235:genetics* / Q000502:physiology; D012732:Sex Determination Analysis / Q000662:veterinary*
chemical_list: D004247:DNA