title: Dynamic proteomics in individual human cells uncovers widespread cell-cycle dependence of nuclear proteins.
abstract: We examined cell cycle-dependent changes in the proteome of human cells by systematically measuring protein dynamics in individual living cells. We used time-lapse microscopy to measure the dynamics of a random subset of 20 nuclear proteins, each tagged with yellow fluorescent protein (YFP) at its endogenous chromosomal location. We synchronized the cells in silico by aligning protein dynamics in each cell between consecutive divisions. We observed widespread (40%) cell-cycle dependence of nuclear protein levels and detected previously unknown cell cycle-dependent localization changes. This approach to dynamic proteomics can aid in discovery and accurate quantification of the extensive regulation of protein concentration and localization in individual living cells.
authors.affiliation: Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Alex
authors.initials: A
authors.lastname: Sigal
authors.forename: Ron
authors.initials: R
authors.lastname: Milo
authors.forename: Ariel
authors.initials: A
authors.lastname: Cohen
authors.forename: Naama
authors.initials: N
authors.lastname: Geva-Zatorsky
authors.forename: Yael
authors.initials: Y
authors.lastname: Klein
authors.forename: Inbal
authors.initials: I
authors.lastname: Alaluf
authors.forename: Naamah
authors.initials: N
authors.lastname: Swerdlin
authors.forename: Natalie
authors.initials: N
authors.lastname: Perzov
authors.forename: Tamar
authors.initials: T
authors.lastname: Danon
authors.forename: Yuvalal
authors.initials: Y
authors.lastname: Liron
authors.forename: Tal
authors.initials: T
authors.lastname: Raveh
authors.forename: Anne E
authors.initials: AE
authors.lastname: Carpenter
authors.forename: Galit
authors.initials: G
authors.lastname: Lahav
authors.forename: Uri
authors.initials: U
authors.lastname: Alon
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000502:physiology*; D002999:Clone Cells; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008164:Luminescent Proteins / Q000737:chemistry; D009687:Nuclear Proteins / Q000737:chemistry* / Q000502:physiology*; D019151:Peptide Library; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D019151:Peptide Library; C054562:yellow fluorescent protein, Bacteria