title: Optical coherence tomography compared with histology of the main pancreatic duct structure in normal and pathological conditions: an 'ex vivo study'.
abstract: OBJECTIVE
  Optical coherence tomography permits high-resolution imaging of tissue microstructures by a probe inserted into the main pancreatic duct through a standard ERCP catheter. The aim of this study was to compare optical coherence tomography images of the main pancreatic duct with histology and identify the optical coherence tomography pattern of the normal and pathological structure of the main pancreatic duct.
  
  
  METHODS
  Multiple sections of neoplastic and non-neoplastic segments of 10 consecutive surgical pancreatic specimens obtained from patients with pancreatic head adenocarcinoma were investigated by optical coherence tomography scanning within 1h of resection. One hundred optical coherence tomography findings were then compared with the corresponding histopathological diagnoses.
  
  
  RESULTS
  Main pancreatic duct wall architecture appeared at optical coherence tomography investigation as a three-layer structure with a different back-scattered signal from each layer. Optical coherence tomography imaging was concordant with histology in 81.8% and 18.75% of sections with normal tissue and chronic inflammatory changes. The K statistic between the two procedures was equal to 0.059 for non-neoplastic main pancreatic duct wall appearance. In all neoplastic sections optical coherence tomography showed a subverted layer architecture with heterogeneous back-scattering of the signal and was concordant with histology.
  
  
  CONCLUSIONS
  Optical coherence tomography provided images of main pancreatic duct wall structure that were concordant with histology in 100% of cases in presence of neoplastic ductal changes and did not have false-positive or negative results. Optical coherence tomography images were also concordant with histology in about 80% of cases with normal main pancreatic duct structure; however, the differential diagnosis between normal tissue and chronic pancreatitis or dysplastic changes appeared very difficult.
authors.affiliation: Division of Gastroenterology, Vita-Salute-San Raffaele University, IRCCS San Raffaele Hospital, Milan, Italy. testoni.pieralberto@hsr.it
authors.forename: P A
authors.initials: PA
authors.lastname: Testoni
authors.forename: B
authors.initials: B
authors.lastname: Mangiavillano
authors.forename: L
authors.initials: L
authors.lastname: Albarello
authors.forename: A
authors.initials: A
authors.lastname: Mariani
authors.forename: P G
authors.initials: PG
authors.lastname: Arcidiacono
authors.forename: E
authors.initials: E
authors.lastname: Masci
authors.forename: C
authors.initials: C
authors.lastname: Doglioni
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000368:Aged; D005260:Female; D006801:Humans; D007249:Inflammation / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010183:Pancreatic Ducts / Q000473:pathology* / Q000648:ultrastructure*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D011446:Prospective Studies; D041623:Tomography, Optical Coherence