Overexpression of GbERF confers alteration of ethylene-responsive gene expression and enhanced resistance to Pseudomonas syringae in transgenic tobacco.
GbERF belongs to the ERF (ethylene responsive factor) family of transcription factors and regulates the GCC-box containing pathogen-related (PR) genes in the ethylene signal transduction pathway. To study the function of GbERF in the process of biotic stress, transgenic tobacco plants expressing GbERF were generated. Overexpression of GbERF did not change transgenic plant's phenotype and endogenous ethylene level. However, the expression profile of some ethylene-inducible GCC-box and non-GCC-box containing genes was altered, such as PR1b, PR2, PR3, PR4, Osmotin, CHN50, ACC oxidase and ACC synthase genes. These data indicate that the cotton GbERF could act as a transcriptional activator or repressor to regulate the differential expression of ethylene-inducible genes via GCC and non-GCC cis-elements. Moreover, the constitutive expression of GbERF in transgenic tobacco enhanced the plant's resistance to Pseudomonas syringae pv tabaci infection. In conclusion, GbERF mediates the expression of a wide array of PR and ethylene-responsive genes and plays an important role in the plant's response to biotic stress.
Qin
Jie
J
Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology, R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shaghai, People's Republic of China.
Zuo
Kaijing
K
Zhao
Jingya
J
Ling
Hua
H
Cao
Youfang
Y
Qiu
Chengxiang
C
Li
Fupeng
F
Sun
Xiaofen
X
Tang
Kexuan
K
D001483:Base Sequence; D005030:Ethylenes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000472:pathogenicity*; D012639:Seeds / Q000254:growth & development; D015398:Signal Transduction / Q000502:physiology; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic
D005030:Ethylenes; D009687:Nuclear Proteins; D010940:Plant Proteins; D014157:Transcription Factors; C036216:ethylene