title: Characterization of TRIM5alpha trimerization and its contribution to human immunodeficiency virus capsid binding.
abstract: The coiled-coil domain of the tripartite motif (TRIM) family protein TRIM5alpha is required for trimerization and function as an antiretroviral restriction factor. Unlike the coiled-coil regions of other related TRIM proteins, the coiled coil of TRIM5alpha is not sufficient for multimerization. The linker region between the coiled-coil and B30.2 domains is necessary for efficient TRIM5alpha trimerization. Most of the hydrophilic residues predicted to be located on the surface-exposed face of the coiled coil can be altered without compromising TRIM5alpha antiviral activity against human immunodeficiency virus (HIV-1). However, changes that disrupt TRIM5alpha trimerization proportionately affect the ability of TRIM5alpha to bind HIV-1 capsid complexes. Therefore, TRIM5alpha trimerization makes a major contribution to its avidity for the retroviral capsid, and to the ability to restrict virus infection.
authors.affiliation: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Hassan
authors.initials: H
authors.lastname: Javanbakht
authors.forename: Wen
authors.initials: W
authors.lastname: Yuan
authors.forename: Darwin F
authors.initials: DF
authors.lastname: Yeung
authors.forename: Byeongwoon
authors.initials: B
authors.lastname: Song
authors.forename: Felipe
authors.initials: F
authors.lastname: Diaz-Griffero
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.forename: Matthew
authors.initials: M
authors.lastname: Stremlau
authors.forename: Joseph
authors.initials: J
authors.lastname: Sodroski
mesh_terms: D000090103:Antiviral Restriction Factors; D002213:Capsid / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D009154:Mutation; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases
chemical_list: D000090103:Antiviral Restriction Factors; D002352:Carrier Proteins; D000070584:Tripartite Motif Proteins; C477346:TRIM5 protein, human; D044767:Ubiquitin-Protein Ligases