Ondamtanggamibang protects neurons from oxidative stress with induction of heme oxygenase-1.
Ondamtanggamibang (ODG) has been used as a remedy to treat psychological anxiety and depression in Oriental medicine. In this study, we found the protective effects of ODG against oxidative stress by induction of the antioxidative enzyme, heme oxygenase (HO)-1 in neuronal PC12 cells. Pretreatment with ODG extract protected neuronal cells from damage induced by H(2)O(2) and 6-hydroxydopamine (6-OHDA), but simultaneous treatment with ODG extract did not. ODG also inhibited the intracellular reactive oxygen species elevation by H(2)O(2) and 6-OHDA. ODG stimulation strongly induced the expression of HO-1 in PC12 cells. The protective effect of ODG extract on oxidative stress-induced damage was suppressed by HO inhibitor, zinc protoporphyrin IX (ZnPP-IX). Taken together, these data suggest that ODG treatment has potential protective effects in neuronal cells under oxidative stress.
Lee
Myeong Soo
MS
Center for Integrative Medicine, Institute of Medical Science, Wonkwang University, Iksan 570-749, Republic of Korea.
Lee
Jienny
J
Kwon
Dae Young
DY
Kim
Myung-Sunny
MS
D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D029001:Herbal Medicine; D006861:Hydrogen Peroxide / Q000633:toxicity; D015151:Immunoblotting; D007723:Korea; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects*; D016627:Oxidopamine / Q000633:toxicity; D016716:PC12 Cells; D010936:Plant Extracts / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011524:Protoporphyrins / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D014151:Anti-Anxiety Agents; D010936:Plant Extracts; D011500:Protein Synthesis Inhibitors; D011524:Protoporphyrins; D017382:Reactive Oxygen Species; C017803:zinc protoporphyrin; D003609:Dactinomycin; D016627:Oxidopamine; D003513:Cycloheximide; D006861:Hydrogen Peroxide; D051547:Heme Oxygenase-1