The Bacillus anthracis cholesterol-dependent cytolysin, Anthrolysin O, kills human neutrophils, monocytes and macrophages.
BACKGROUND
Bacillus anthracis is an animal and human pathogen whose virulence is characterized by lethal and edema toxin, as well as a poly-glutamic acid capsule. In addition to these well characterized toxins, B. anthracis secretes several proteases and phospholipases, and a newly described toxin of the cholesterol-dependent cytolysin (CDC) family, Anthrolysin O (ALO).


RESULTS
In the present studies we show that recombinant ALO (rALO) or native ALO, secreted by viable B. anthracis, is lethal to human primary polymorphonuclear leukocytes (PMNs), monocytes, monocyte-derived macrophages (MDMs), lymphocytes, THP-1 monocytic human cell line and ME-180, Detroit 562, and A549 epithelial cells by trypan blue exclusion or lactate dehydrogenase (LDH) release viability assays. ALO cytotoxicity is dose and time dependent and susceptibility to ALO-mediated lysis differs between cell types. In addition, the viability of monocytes and hMDMs was assayed in the presence of vegetative Sterne strains 7702 (ALO+), UT231 (ALO-), and a complemented strain expressing ALO, UT231 (pUTE544), and was dependent upon the expression of ALO. Cytotoxicity of rALO is seen as low as 0.070 nM in the absence of serum. All direct cytotoxic activity is inhibited by the addition of cholesterol or serum concentration as low as 10%.


CONCLUSIONS
The lethality of rALO and native ALO on human monocytes, neutrophils, macrophages and lymphocytes supports the idea that ALO may represent a previously unidentified virulence factor of B. anthracis. The study of other factors produced by B. anthracis, along with the major anthrax toxins, will lead to a better understanding of this bacterium's pathogenesis, as well as provide information for the development of antitoxin vaccines for treating and preventing anthrax.
Mosser
Elise M
EM
Department of Microbiology and Immunology, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, USA. emb39@drexel.edu
Rest
Richard F
RF
D001408:Bacillus anthracis / Q000378:metabolism / Q000472:pathogenicity*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D003603:Cytotoxins / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D011994:Recombinant Proteins / Q000633:toxicity; D014343:Trypan Blue
D001426:Bacterial Proteins; D003603:Cytotoxins; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; C498000:anthrolysin O, Bacillus anthracis; D002784:Cholesterol; D014343:Trypan Blue