title: Detection of group a human rotavirus G9 genotype circulating in Córdoba, Argentina, as early as 1980.
abstract: The incidence of human rotavirus G types was determined over a 25-year period (1979-2003) by using reverse transcription-PCR (RT-PCR) to examine 519 stool specimens found to be positive for rotavirus by enzyme linked immunosorbent assay (ELISA) or polyacrylamide gel electrophoresis (PAGE). These stool samples were obtained from children under 3 years old who had been treated for acute diarrhea at public hospitals in Córdoba, Argentina. The present study describes the continued circulation of the common human G types G1 (53.8%), G2 (10.2%), G3 (4.4%), and G4 (27%), and also the detection of the unusual types G8 (0.5%) and G9 (4.2%). Genotype G9 was detected during the 1980-1988 and 1997-2003 periods at relatively low rates. Rotavirus G types distribution was independent of age (1-18 months), gender or out-patient or in-patient status. Unexpectedly, 44.6% of mixed infections were detected, involving common and unusual genotypes. Overall, 95.4% of the typed strains belonged to the most prevalent human serotypes (G1-G4) but the detection of G9 infection throughout this study period highlights the importance of this serotype as a human pathogen.
authors.affiliation: Instituto de Virología Dr. J.M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n Agencia 4-Ciudad Universitaria, Córdoba, Argentina.
authors.forename: P A
authors.initials: PA
authors.lastname: Barril
authors.forename: L C
authors.initials: LC
authors.lastname: Martinez
authors.forename: M O
authors.initials: MO
authors.lastname: Giordano
authors.forename: A A
authors.initials: AA
authors.lastname: Castello
authors.forename: R P
authors.initials: RP
authors.lastname: Rota
authors.forename: M B
authors.initials: MB
authors.lastname: Isa
authors.forename: G
authors.initials: G
authors.lastname: Masachessi
authors.forename: L J
authors.initials: LJ
authors.lastname: Ferreyra
authors.forename: G
authors.initials: G
authors.lastname: Glikmann
authors.forename: S V
authors.initials: SV
authors.lastname: Nates
mesh_terms: D000375:Aging; D001118:Argentina / Q000453:epidemiology; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D013997:Time Factors