title: Comparison of detection methods for Toxoplasma gondii in naturally and experimentally infected swine.
abstract: Results from recent serological surveys and epidemiological studies show that pigs raised in a variety of management systems can be carriers of the tissue cyst stage of Toxoplasma gondi. This parasite can be transmitted to humans through the consumption of improperly prepared pork, making detection and removal of infected swine carcasses from the food chain an important food safety issue. Several methods are available for detection of T. gondii infected swine, including serological assays, polymerase chain reaction, and animal bioassays. The aim of the present study was to compare the detection sensitivities of six of these commonly used methods for detection of T. gondii infection in tissues from naturally and experimentally infected pigs. The results indicate that a serum-based ELISA is the most sensitive method, of those tested, for detection of T. gondii infected swine.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC-East Beltsville, MD 20705, USA. dhill@anri.barc.usda.gov
authors.forename: Dolores E
authors.initials: DE
authors.lastname: Hill
authors.forename: Sreekumar
authors.initials: S
authors.lastname: Chirukandoth
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: Joan K
authors.initials: JK
authors.lastname: Lunney
authors.forename: H Ray
authors.initials: HR
authors.lastname: Gamble
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001681:Biological Assay / Q000379:methods / Q000662:veterinary; D002353:Carrier State / Q000662:veterinary; D002415:Cats; D004197:Disease Reservoirs / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005506:Food Contamination; D016266:Food Parasitology; D010270:Parasite Egg Count / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis*
chemical_list: D000913:Antibodies, Protozoan