Circulating gamma delta T cells in response to Salmonella enterica serovar enteritidis exposure in chickens.
gammadelta T cells are considered crucial to the outcome of various infectious diseases. The present study was undertaken to characterize gammadelta (T-cell receptor 1(+) [TCR1(+)]) T cells phenotypically and functionally in avian immune response. Day-old chicks were orally immunized with Salmonella enterica serovar Enteritidis live vaccine or S. enterica serovar Enteritidis wild-type strain and infected using the S. enterica serovar Enteritidis wild-type strain on day 44 of life. Between days 3 and 71, peripheral blood was examined flow cytometrically for the occurrence of gammadelta T-cell subpopulations differentiated by the expression of T-cell antigens. Three different TCR1(+) cell populations were found to display considerable variation regarding CD8alpha antigen expression: (i) CD8alpha(+high) TCR1(+) cells, (ii) CD8alpha(+dim) TCR1(+) cells, and (iii) CD8alpha(-) TCR1(+) cells. While most of the CD8alpha(+high) TCR1(+) cells expressed the CD8alphabeta heterodimeric antigen, the majority of the CD8alpha(+dim) TCR1(+) cells were found to express the CD8alphaalpha homodimeric form. After immunization, a significant increase of CD8alphaalpha(+high) gammadelta T cells was observed within the CD8alpha(+high) TCR1(+) cell population. Quantitative reverse transcription-PCR revealed reduced interleukin-7 receptor alpha (IL-7Ralpha) and Bcl-x expression and elevated IL-2Ralpha mRNA expression of the CD8alphaalpha(+high) gammadelta T cells. Immunohistochemical analysis demonstrated a significant increase of CD8alpha(+) and TCR1(+) cells in the cecum and spleen and a decreased percentage of CD8beta(+) T cells in the spleen after Salmonella immunization. After infection of immunized animals, immune reactions were restricted to intestinal tissue. The study showed that Salmonella immunization of very young chicks is accompanied by an increase of CD8alphaalpha(+high) gammadelta T cells in peripheral blood, which are probably activated, and thus represent an important factor for the development of a protective immune response to Salmonella organisms in chickens.
Berndt
Angela
A
Friedrich-Loeffler-Institute, Institute of Molecular Pathogenesis, Naumburger Str. 96a, D-07743 Jena, Germany. angela.berndt@fli.bund.de
Pieper
Jana
J
Methner
Ulrich
U
D000818:Animals; D018106:CD28 Antigens / Q000097:blood / Q000235:genetics; D016827:CD8 Antigens / Q000097:blood / Q000235:genetics; D002478:Cells, Cultured; D002645:Chickens; D053222:Fas Ligand Protein; D053645:Interleukin-2 Receptor alpha Subunit; D008562:Membrane Glycoproteins / Q000097:blood / Q000235:genetics; D011201:Poultry Diseases / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis / Q000097:blood*; D015375:Receptors, Interleukin-2 / Q000097:blood / Q000235:genetics; D020395:Receptors, Interleukin-7 / Q000097:blood / Q000235:genetics; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D048069:Tumor Necrosis Factors / Q000097:blood / Q000235:genetics; D051020:bcl-X Protein / Q000097:blood / Q000235:genetics; D019014:fas Receptor / Q000097:blood / Q000235:genetics
D018106:CD28 Antigens; D016827:CD8 Antigens; C431698:CD8alpha antigen; D053222:Fas Ligand Protein; D053645:Interleukin-2 Receptor alpha Subunit; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2; D020395:Receptors, Interleukin-7; D048069:Tumor Necrosis Factors; D051020:bcl-X Protein; D019014:fas Receptor; C415350:interleukin-7 receptor, alpha chain