Characterization and phylogenetic analysis of Brazilian chicken anaemia virus.
Chicken anaemia virus (CAV) was detected by a Nested-PCR assay in field samples from different regions of Brazil. The 539 bp amplified fragments of vp1 gene from 44 field samples were sequenced and 10 new nucleotide sequences of CAV were observed. These sequences were phylogenetically analysed by Mega2 using neighbour joining distance methods with 1000 bootstrap replications. Phylogenetic analysis did not show correlation between CAV pathology pattern and genetic groups. The 10 nucleotide sequences of the Brazilian samples were also analysed together with 30 sequences of CAV strains previously described from other countries. The genetic variability observed was not related to the geographical distribution. Amino acid substitutions were detected at 9 positions of the Brazilian sequences and two of them had not been observed before, (65)R replacing the Q residue and (98)F replacing Y residue.
Simionatto
Simone
S
Laboratório de Virologia, Faculdade de Veterinária (FAVET), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 9090, Cep: 91540-000, Porto Alegre, RS, Brasil.
Lima-Rosa
Carlos André da Veiga
CA
Binneck
Eliseu
E
Ravazzolo
Ana Paula
AP
Canal
Cláudio Wageck
CW
D000595:Amino Acid Sequence; D000818:Animals; D001938:Brazil; D017727:Chicken anemia virus / Q000737:chemistry* / Q000235:genetics*; D002645:Chickens / Q000821:virology; D018173:Circoviridae Infections / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny