Palindromes and genomic stress fractures: bracing and repairing the damage.
DNA palindromes are a source of instability in eukaryotic genomes but remain under-investigated because they are difficult to study. Nonetheless, progress in the last year or so has begun to form a coherent picture of how DNA palindromes cause damage in eukaryotes and how this damage is opposed by cellular mechanisms. In yeast, the features of double strand DNA interruptions that appear at palindromic sites in vivo suggest that a resolvase-type activity creates the fractures by attacking a palindrome after it extrudes into a cruciform structure. Induction of DNA breaks in this fashion could be deterred through a Center-Break palindrome revision process as investigated in detail in mice. The MRX/MRN likely plays a pivotal role in prevention of palindrome-induced genome damage in eukaryotes.
Lewis
Susanna M
SM
Graduate Department of Molecular and Medical Genetics, University of Toronto, Ont., Canada. susanna@sickkids.on.ca
Coté
Atina G
AG
D000818:Animals; D001483:Base Sequence; D043171:Chromosomal Instability; D019457:Chromosome Breakage; D033481:Chromosomes, Mammalian; D004260:DNA Repair; D015894:Genome, Human; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data