Proteomic analysis of Agrobacterium tumefaciens response to the Vir gene inducer acetosyringone.
Agrobacterium tumefaciens causes crown gall disease in a wide range of plants by transforming plants through the transfer and integration of its transferred DNA (T-DNA) into the host genome. In the present study, we used two-dimensional gel electrophoresis to examine the protein expression profiles of A. tumefaciens in response to the phenolic compound acetosyringone (AS), a known plant-released virulence (vir) gene inducer. Using mass spectrometry, we identified 11 proteins consisting of 9 known AS-induced Vir proteins and 2 newly discovered AS-induced proteins, an unknown protein Y4mC (Atu6162) and a small heat shock protein HspL (Atu3887). Further expression analysis revealed that the AS-induced expression of Y4mC and HspL is regulated by the VirA/VirG two-component system. This report presents the first proteomics study successfully identifying both known and new AS-induced proteins that are implicated in Agrobacterium virulence.
Lai
Erh-Min
EM
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. emlai@gate.sinica.edu.tw
Shih
Hsiao-Wei
HW
Wen
Sy-Ray
SR
Cheng
Min-Wen
MW
Hwang
Hau-Hsuan
HH
Chiu
Shih-Hua
SH
D000098:Acetophenones / Q000494:pharmacology*; D016960:Agrobacterium tumefaciens / Q000187:drug effects / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
D000098:Acetophenones; D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors; C051667:acetosyringone