Naturally occurring extended-spectrum cephalosporinases in Escherichia coli.
Genetic and functional characterization of the cephalosporinases produced by 65 clonally unrelated clinical Escherichia coli isolates revealed genetic diversity of the ampC genes and showed that Gln287, Cys287, Pro296, Leu298, and Phe350 substitutions were involved in extension of the hydrolysis spectrum to include ceftazidime and cefepime.
Mammeri
Hedi
H
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris Sud, 94275 Le Kremlin-Bicêtre, France
Poirel
Laurent
L
Fortineau
Nicolas
N
Nordmann
Patrice
P
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000077723:Cefepime; D002442:Ceftazidime / Q000378:metabolism / Q000494:pharmacology*; D002510:Cephalosporinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002511:Cephalosporins / Q000378:metabolism / Q000494:pharmacology*; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002511:Cephalosporins; D000077723:Cefepime; D002442:Ceftazidime; D002510:Cephalosporinase; C048795:AmpC beta-lactamases; D001618:beta-Lactamases