Expression of T-STAR gene is associated with regulation of telomerase activity in human colon cancer cell line HCT-116.
OBJECTIVE
To investigate the effects on telomerase activity of transfection of human T-STAR gene full-length sense cDNA or partial antisense cDNA into human colon cancer cell line HCT-116.


METHODS
mRNA and protein expression levels of T-STAR gene were determined by RT-PCR and western blot, and telomerase activity was measured by PCR-ELISA, after transfection of T-STAR sense or antisense gene into HCT-116 cells with lipofectamine.


RESULTS
T-STAR gene expression was enhanced or knocked down both at mRNA and protein levels, and telomerase activity was significantly increased or decreased.


CONCLUSIONS
The T-STAR gene may participate in regulation of telomerase activity in human colon cancer HCT-116 cells in a parallel fashion.
Zhang
Ling
L
Department of Disease Prevention and Health Protection, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
Guo
Lian
L
Peng
Yong
Y
Chen
Bing
B
D016373:DNA, Antisense; D018076:DNA, Complementary; D045325:HCT116 Cells; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism*; D014162:Transfection
D016373:DNA, Antisense; D018076:DNA, Complementary; C118777:KHDRBS3 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D019098:Telomerase