title: Proteomic analysis of Agrobacterium tumefaciens response to the Vir gene inducer acetosyringone.
abstract: Agrobacterium tumefaciens causes crown gall disease in a wide range of plants by transforming plants through the transfer and integration of its transferred DNA (T-DNA) into the host genome. In the present study, we used two-dimensional gel electrophoresis to examine the protein expression profiles of A. tumefaciens in response to the phenolic compound acetosyringone (AS), a known plant-released virulence (vir) gene inducer. Using mass spectrometry, we identified 11 proteins consisting of 9 known AS-induced Vir proteins and 2 newly discovered AS-induced proteins, an unknown protein Y4mC (Atu6162) and a small heat shock protein HspL (Atu3887). Further expression analysis revealed that the AS-induced expression of Y4mC and HspL is regulated by the VirA/VirG two-component system. This report presents the first proteomics study successfully identifying both known and new AS-induced proteins that are implicated in Agrobacterium virulence.
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. emlai@gate.sinica.edu.tw
authors.forename: Erh-Min
authors.initials: EM
authors.lastname: Lai
authors.forename: Hsiao-Wei
authors.initials: HW
authors.lastname: Shih
authors.forename: Sy-Ray
authors.initials: SR
authors.lastname: Wen
authors.forename: Min-Wen
authors.initials: MW
authors.lastname: Cheng
authors.forename: Hau-Hsuan
authors.initials: HH
authors.lastname: Hwang
authors.forename: Shih-Hua
authors.initials: SH
authors.lastname: Chiu
mesh_terms: D000098:Acetophenones / Q000494:pharmacology*; D016960:Agrobacterium tumefaciens / Q000187:drug effects / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000098:Acetophenones; D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors; C051667:acetosyringone