Purification of coenzyme Q10 from fermentation extract: high-speed counter-current chromatography versus silica gel column chromatography.
High-speed counter-current chromatography (HSCCC) is applied to the purification of coenzyme Q(10) (CoQ(10)) for the first time. CoQ(10) was obtained from a fermentation broth extract. A non-aqueous two-phase solvent system composed of heptane-acetonitrile-dichloromethane (12:7:3.5, v/v/v) was selected by analytical HSCCC and used for purification of CoQ(10) from 500 mg of the crude extract. The separation yielded 130 mg of CoQ(10) at an HPLC purity of over 99%. The overall results of the present studies show the advantages of HSCCC over an alternative of silica gel chromatography followed by recrystallization. These advantages extend to higher purity (97.8% versus 93.3%), recovery (88% versus 74.3%) and yield (26.4% versus 23.4%). An effort to avoid the toxic, expensive solvent CH(2)Cl(2) was unsuccessful, but at least its percentage is low in the solvent system.
Cao
Xue-Li
XL
Beijing Technology and Business University, School of Chemical and Environmental Engineering, Beijing Key Lab of Plant Resource Research and Development, Beijing 100037, China.
Xu
Ya-Tao
YT
Zhang
Guang-Ming
GM
Xie
Sheng-Meng
SM
Dong
Ying-Mao
YM
Ito
Yoichiro
Y
D016960:Agrobacterium tumefaciens / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods*; D003067:Coenzymes; D003377:Countercurrent Distribution / Q000379:methods*; D005285:Fermentation; D058428:Silica Gel; D012822:Silicon Dioxide; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000302:isolation & purification
D003067:Coenzymes; D014451:Ubiquinone; D058428:Silica Gel; D012822:Silicon Dioxide; C024989:coenzyme Q10