Somite formation and expression of MyoD, myogenin and myosin in Atlantic halibut (Hippoglossus hippoglossus L.) embryos incubated at different temperatures: transient asymmetric expression of MyoD.
Genes encoding the myogenic regulating factors MyoD and myogenin and the structural muscle proteins myosin light chain 2 (MyLC2) and myosin heavy chain (MyHC) were isolated from juvenile Atlantic halibut (Hippoglossus hippoglossus L.). The impact of temperature on their temporal and spatial expression during somitogenesis were examined by incubating halibut embryos at 4, 6 and 8 degrees C, and regularly sampling for whole-mount in situ hybridisation and reverse transcription (RT)-PCR. There were no significant effects of temperature on the onset of somitogenesis or number of somites at hatching. The rate of somite formation increased with increasing temperature, and the expression of MyoD, myogenin and MyHC followed the cranial-to-caudal somite formation. Hence, no significant effect of temperature on the spatial and temporal expression of the genes studied was found in relation to somite stage. MyoD, which has subsequently been shown to encode the MyoD2 isoform, displayed a novel bilaterally asymmetric expression pattern only in white muscle precursor cells during early halibut somitogenesis. The expression of myogenin resembled that previously described for other fish species, and preceded the MyHC expression by approximately five somites. Two MyLC2 cDNA sequences were for the first time described for a flatfish, probably representing embryonic (MyLC2a) and larval/juvenile (MyLC2b) isoforms. Factors regulating muscle determination, differentiation and development have so far mostly been studied in vertebrates with external bilateral symmetry. The findings of the present study suggest that more such investigations of flatfish species could provide valuable information on how muscle-regulating mechanisms work in species with different anatomical, physiological and ecological traits.
Galloway
Trina F
TF
Department of Biology, Brattøra Research Centre, Norwegian University of Science and Technology (NTNU), N-7491 Trondheim, Norway. trine.galloway@biomar.no
Bardal
Tora
T
Kvam
Sylvia N
SN
Dahle
Stine W
SW
Nesse
Gaute
G
Randøl
Markus
M
Kjørsvik
Elin
E
Andersen
Oivind
O
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005432:Flounder / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018257:Helix-Loop-Helix Motifs; D008969:Molecular Sequence Data; D017570:MyoD Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018008:Myogenin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D018994:Myosin Light Chains / Q000235:genetics / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D019170:Somites / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013696:Temperature
D018076:DNA, Complementary; D029941:Fish Proteins; D017570:MyoD Protein; D018008:Myogenin; D018994:Myosin Light Chains; D020033:Protein Isoforms; D018995:Myosin Heavy Chains; D009218:Myosins