title: Centrosome amplification in adult T-cell leukemia and human T-cell leukemia virus type 1 Tax-induced human T cells.
abstract: Centrosomes play pivotal roles in cell polarity, regulation of the cell cycle and chromosomal segregation. Centrosome amplification was recently described as a possible cause of aneuploidy in certain solid tumors and leukemias. ATL is a T-cell malignancy caused by HTLV-1. Although the precise mechanism of cell transformation is unclear, the HTLV-1-encoded protein, Tax, is thought to play a crucial role in leukemogenesis. Here we demonstrate that lymphocytes isolated from patients with ATL show centrosome amplification and that a human T cell line shows centrosome amplification after induction of Tax, which was suppressed by CDK inhibitors. Micronuclei formation was also observed after centrosome amplification in Tax-induced human T cells. These findings suggest that Tax deregulates CDK activity and induces centrosome amplification, which might be associated with cellular transformation by HTLV-1 and chromosomal instability in HTLV-1-infected human T cells.
authors.affiliation: Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8575, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nitta
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kanai
authors.forename: Eiji
authors.initials: E
authors.lastname: Sugihara
authors.forename: Masakazu
authors.initials: M
authors.lastname: Tanaka
authors.forename: Binlian
authors.initials: B
authors.lastname: Sun
authors.forename: Toshiro
authors.initials: T
authors.lastname: Nagasawa
authors.forename: Shunro
authors.initials: S
authors.lastname: Sonoda
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Saya
authors.forename: Masanao
authors.initials: M
authors.lastname: Miwa
mesh_terms: D002472:Cell Transformation, Viral / Q000235:genetics*; D018385:Centrosome / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D016356:Gene Products, tax / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D019169:Jurkat Cells; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000821:virology*
chemical_list: D016356:Gene Products, tax