title: Alteration of serotonin transporter density and activity in fibromyalgia.
abstract: The aim of the study was to evaluate the kinetic parameters of a specific serotonin transporter (SERT) and serotonin uptake in a mentally healthy subset of patients with fibromyalgia. Platelets were obtained from 40 patients and 38 healthy controls. SERT expression and functionality were evaluated through the measurement of [3H]paroxetine binding and the [3H]serotonin uptake itself. The values of maximal membrane binding capacity (Bmax) were statistically lower in the patients than in the healthy volunteers, whereas the dissociation constant (Kd) did not show any statistically significant variations. Moreover, a decrease in the maximal uptake rate of SERT (Vmax) was demonstrated in the platelets of patients, whereas the Michaelis constant (Km) did not show any statistically significant variations. Symptom severity score (tiredness, tender points index and Fibromyalgia Impact Questionnaire) were negatively correlated with Bmax and with Vmax, and positively correlated with Km. A change in SERT seems to occur in fibromyalgic patients, and it seems to be related to the severity of fibromyalgic symptoms.
authors.affiliation: Department of Internal Medicine, Division of Rheumatology, University of Pisa, Via Roma 67-56126 Pisa, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Bazzichi
authors.forename: Gino
authors.initials: G
authors.lastname: Giannaccini
authors.forename: Laura
authors.initials: L
authors.lastname: Betti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Mascia
authors.forename: Laura
authors.initials: L
authors.lastname: Fabbrini
authors.forename: Paola
authors.initials: P
authors.lastname: Italiani
authors.forename: Francesca
authors.initials: F
authors.lastname: De Feo
authors.forename: Tiziana
authors.initials: T
authors.lastname: Giuliano
authors.forename: Camillo
authors.initials: C
authors.lastname: Giacomelli
authors.forename: Alessandra
authors.initials: A
authors.lastname: Rossi
authors.forename: Antonio
authors.initials: A
authors.lastname: Lucacchini
authors.forename: Stefano
authors.initials: S
authors.lastname: Bombardieri
mesh_terms: D000368:Aged; D001792:Blood Platelets / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005356:Fibromyalgia / Q000097:blood* / Q000503:physiopathology; D006801:Humans; D007700:Kinetics; D008875:Middle Aged; D017374:Paroxetine / Q000378:metabolism; D012701:Serotonin / Q000493:pharmacokinetics; D050486:Serotonin Plasma Membrane Transport Proteins / Q000097:blood*; D017367:Selective Serotonin Reuptake Inhibitors / Q000378:metabolism; D012720:Severity of Illness Index; D018434:Sickness Impact Profile; D011795:Surveys and Questionnaires
chemical_list: D017374:Paroxetine; D012701:Serotonin; D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors