Evaluation of deletions in 7q11.2 and 8p12-p21 as prognostic indicators of tumour development following molar pregnancy.
OBJECTIVE
Previous studies have identified loss of chromosomal regions 7p12-q11.2 and 8p12-p21 in choriocarcinoma suggesting that suppressor genes involved in tumour development may be located within these regions. Our objectives were to refine the regions of loss and evaluate these deletions as prognostic indicators of trophoblastic tumour development following molar pregnancy.


METHODS
Fluorescent microsatellite genotyping was used to perform deletion mapping in a series of thirty-nine gestational trophoblastic tumours (GTT) including both choriocarcinoma and placental site trophoblastic tumours.


RESULTS
Significant loss of heterozygosity (LOH) was found for both regions in GTT that originated in non-molar pregnancies. Although no common interval of loss was found in those GTT with LOH for the 7q11.2 region, for the 8p12-p21 locus, markers D8S1731 and NEFL defined a minimal region of loss in all tumours showing LOH. However, complete LOH of either region occurred in only a minority of tumours (20%; chromosome 7: 24%; chromosome 8) suggesting that loss of neither region is likely to be a primary event in the development of GTT. This was further supported by the observation that no deletions were found in either region for the fourteen GTT that followed complete molar pregnancies.


CONCLUSIONS
While we have defined a minimal interval in 8p12-p21 in which tumour suppressor genes involved in GTT are likely to be located, the data suggest that deletions in 7q11.2 or 8p12-p21 are unlikely to be useful prognostic indicators in the management of patients with molar pregnancies.
Burke
Beverley
B
Department of Oncology, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Imperial College London, Charing Cross Hospital, Fulham Palace Road, London, UK.
Sebire
Neil J
NJ
Moss
Jill
J
Hodges
Matthew D
MD
Seckl
Michael J
MJ
Newlands
Edward S
ES
Fisher
Rosemary A
RA
D000328:Adult; D002822:Choriocarcinoma / Q000235:genetics / Q000473:pathology; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D005260:Female; D031901:Gestational Trophoblastic Disease / Q000235:genetics* / Q000473:pathology; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000235:genetics* / Q000473:pathology