title: Arginylation of beta-actin regulates actin cytoskeleton and cell motility.
abstract: Posttranslational arginylation is critical for mouse embryogenesis, cardiovascular development, and angiogenesis, but its molecular effects and the identity of proteins arginylated in vivo are unknown. We found that beta-actin was arginylated in vivo to regulate actin filament properties, beta-actin localization, and lamella formation in motile cells. Arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo. Thus, posttranslational arginylation of a single protein target can regulate its intracellular function, inducing global changes on the cellular level, and may contribute to cardiovascular development and angiogenesis.
authors.affiliation: Department of Animal Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Marina
authors.initials: M
authors.lastname: Karakozova
authors.forename: Marina
authors.initials: M
authors.lastname: Kozak
authors.forename: Catherine C L
authors.initials: CC
authors.lastname: Wong
authors.forename: Aaron O
authors.initials: AO
authors.lastname: Bailey
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Alexander
authors.initials: A
authors.lastname: Mogilner
authors.forename: Henry
authors.initials: H
authors.lastname: Zebroski
authors.forename: Anna
authors.initials: A
authors.lastname: Kashina
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000737:chemistry / Q000378:metabolism*; D002465:Cell Movement; D048430:Cell Shape; D048429:Cell Size; D005347:Fibroblasts; D047468:Immunoprecipitation; D007526:Isoelectric Point; D013058:Mass Spectrometry; D051379:Mice; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D011554:Pseudopodia / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000199:Actins; D020033:Protein Isoforms; D001120:Arginine; D019881:Aminoacyltransferases; C019727:arginyltransferase