The recovery of 13C-labeled oleic acid in rat lymph after administration of long chain triacylglycerols or specific structured triacylglycerols.
BACKGROUND
Consumption of specific structured triacylglycerols, MLM (M = medium chain fatty acid, L = long chain fatty acid), delivers fast energy and long chain fatty acids to the organism.


OBJECTIVE
The purpose of the present study was to compare lymphatic absorption of (13)C-labeled MLM and (13)C-labeled LLL in rats. Stable isotope labeling enables the separation of the endogenous and exogenous fatty acids.


METHODS
Lymph was collected during 24 h following administration of MLM or LLL. Lymph fatty acid composition and (13)C-enrichment were determined and quantified by gas chromatography combustion isotope ratio mass spectrometry.


RESULTS
The recovery of 18:1n-9 was higher after administration of LLL compared with MLM (58.1% +/- 7.4% and 29.1% +/- 3.9%, respectively, P < 0.001). This may be due to a higher chylomicron formation stimulated by a higher amount of long chain fatty acids in the intestine after LLL compared with MLM administration. This was confirmed by the tendencies of higher lymphatic transport of endogenous fatty acids.


CONCLUSIONS
The study revealed a higher lymphatic recovery of the administered long chain fatty acids after LLL compared with MLM consumption.
Vistisen
Bodil
B
BioCentrum-DTU, Section of Biochemistry & Nutrition, Technical University of Denmark, Lyngby, Denmark. bvistisen@mfi.ku.dk
Mu
Huiling
H
Høy
Carl-Erik
CE
D000284:Administration, Oral; D000818:Animals; D001692:Biological Transport; D002247:Carbon Isotopes; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007408:Intestinal Absorption / Q000187:drug effects; D008196:Lymph / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D019301:Oleic Acid / Q000008:administration & dosage / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D014280:Triglycerides / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics
D002247:Carbon Isotopes; D004041:Dietary Fats; D014280:Triglycerides; D019301:Oleic Acid