Triamcinolone acetonide protects auditory hair cells from 4-hydroxy-2,3-nonenal (HNE) ototoxicity in vitro.
CONCLUSIONS
Triamcinolone acetonide crystalline suspension (e.g. Volon A) was not ototoxic to the auditory hair cells present within organ of Corti explants and protected them from an ototoxic molecule, i.e. 4-hydroxy-2,3-nonenal (HNE), that is produced within the organ of Corti as a result of oxidative stress-induced damage.


OBJECTIVE
To test the corticosteroid, triamcinolone acetonide, for ototoxicity and otoprotective capacity in organ of Corti explants.


METHODS
Organ of Corti explants excised from 4-day-old rats were the test system, HNE was the ototoxin challenge. Hair cell integrity counts were performed with fluorescent microscopy on fixed explants stained with FITC-labeled phalloidin. Statistical significance was set at p<0.05.


RESULTS
Triamcinolone acetonide did not affect hair cell integrity in the organ of Corti explants and it provided a high level of protection of hair cells against the ototoxic effects of a damaging level of HNE as determined by hair cell density counts.
Guzman
Jose
J
University of Miami Ear Institute, Department of Otolaryngology, Miller School of Medicine University of Miami, Miami, FL 33136, USA.
Ruiz
Jose
J
Eshraghi
Adrien A
AA
Polak
Marek
M
Garnham
Carolyn
C
Balkany
Thomas J
TJ
Van de Water
Thomas R
TR
D000447:Aldehydes / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000633:toxicity; D000818:Animals; D015834:Cochlear Diseases / Q000188:drug therapy / Q000517:prevention & control*; D005938:Glucocorticoids / Q000494:pharmacology* / Q000627:therapeutic use; D006198:Hair Cells, Auditory / Q000166:cytology / Q000187:drug effects*; D066298:In Vitro Techniques; D009925:Organ of Corti / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D014222:Triamcinolone Acetonide / Q000494:pharmacology* / Q000627:therapeutic use
D000447:Aldehydes; D005938:Glucocorticoids; D014222:Triamcinolone Acetonide; C027576:4-hydroxy-2-nonenal