title: Gene expression profile related to the progression of preneoplastic nodules toward hepatocellular carcinoma in rats.
abstract: In this study, we investigated the time course gene expression profile of preneoplastic nodules and hepatocellular carcinomas (HCC) to define the genes implicated in cancer progression in a resistant hepatocyte model. Tissues that included early nodules (1 month, ENT-1), persistent nodules (5 months, ENT-5), dissected HCC (12 months), and normal livers (NL) from adult rats were analyzed by cDNA arrays including 1185 rat genes. Differential genes were derived in each type of sample (n = 3) by statistical analysis. The relationship between samples was described in a Venn diagram for 290 genes. From these, 72 genes were shared between tissues with nodules and HCC. In addition, 35 genes with statistical significance only in HCC and with extreme ratios were identified. Differential expression of 11 genes was confirmed by comparative reverse transcription-polymerase chain reaction, whereas that of 2 genes was confirmed by immunohistochemistry. Members involved in cytochrome P450 and second-phase metabolism were downregulated, whereas genes involved in glutathione metabolism were upregulated, implicating a possible role of glutathione and oxidative regulation. We provide a gene expression profile related to the progression of nodules into HCC, which contributes to the understanding of liver cancer development and offers the prospect for chemoprevention strategies or early treatment of HCC.
authors.affiliation: Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del IPN, Mexico, DF, Mexico.
authors.forename: Julio Isael
authors.initials: JI
authors.lastname: Pérez-Carreón
authors.forename: Cristina
authors.initials: C
authors.lastname: López-García
authors.forename: Samia
authors.initials: S
authors.lastname: Fattel-Fazenda
authors.forename: Evelia
authors.initials: E
authors.lastname: Arce-Popoca
authors.forename: Leticia
authors.initials: L
authors.lastname: Alemán-Lazarini
authors.forename: Sergio
authors.initials: S
authors.lastname: Hernández-García
authors.forename: Véronique
authors.initials: V
authors.lastname: Le Berre
authors.forename: Sergueï
authors.initials: S
authors.lastname: Sokol
authors.forename: Jean Marie
authors.initials: JM
authors.lastname: Francois
authors.forename: Saúl
authors.initials: S
authors.lastname: Villa-Treviño
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018076:DNA, Complementary / Q000378:metabolism; D018450:Disease Progression; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008954:Models, Biological; D011230:Precancerous Conditions / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors
chemical_list: D018076:DNA, Complementary