Neuroglobin mRNA expression after transient global brain ischemia and prolonged hypoxia in cell culture.
Neuroglobin is a nerve-specific respiratory protein that has been proposed to play an important role in the protection of brain neurons from ischemic and hypoxic injuries. Here, we investigated the regulation of neuroglobin expression after transient global ischemia in the rat brain using mRNA in situ hybridization and under hypoxic stress in cultured neuronal cell lines (PC12, HN33) by quantitative RT-PCR. While neuroglobin mRNA expression was significantly enhanced in cell culture after severe prolonged hypoxia (0-1% O2 for 24 h), we did not find any significant increases in neuroglobin mRNA levels in the rat brain after transient global ischemia. Vegf and Glut1 mRNAs showed increases in the hippocampus as expected. Therefore, it is unlikely that neuroglobin is instrumental in the acute response of neurons to hypoxic or ischemic insults, for which the mammalian brain is not adapted.
Schmidt-Kastner
Rainald
R
Department of Neurology, University of Miami School of Medicine, Miami, FL 33101, USA.
Haberkamp
Mark
M
Schmitz
Christoph
C
Hankeln
Thomas
T
Burmester
Thorsten
T
D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005914:Globins / Q000096:biosynthesis*; D006801:Humans; D017403:In Situ Hybridization; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D000078787:Neuroglobin; D009474:Neurons / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology; D016716:PC12 Cells; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D005914:Globins