title: Altered beta-amyloid precursor protein isoforms in Mexican Alzheimer's Disease patients.
abstract: OBJECTIVE
  To determine the beta-amyloid precursor protein (betaAPP) isoforms ratio as a risk factor for Alzheimer's Disease and to assess its relationship with demographic and genetic variables of the disease.
  
  
  METHODS
  Blood samples from 26 patients fulfilling NINCDS-ADRDA diagnostic criteria for AD and 46 healthy control subjects were collected for Western blotting for betaAPP. A ratio of betaAPP isoforms, in optical densities, between the upper band (130 Kd) and the lower bands (106-110 Kd) was obtained. Odds ratios were obtained to determine risk factor of this component.
  
  
  RESULTS
  betaAPP ratio on AD subjects was lower than that of control subjects: 0.3662 +/- 0.1891 vs. 0.6769 +/- 0.1021 (mean +/- SD, p<0.05). A low betaAPP ratio (<0.6) showed an OR of 4.63 (95% CI 1.45-15.33). When onset of disease was taken into account, a betaAPP ratio on EOAD subjects of 0.3965 +/- 0.1916 was found vs. 0.3445 +/- 0.1965 on LOAD subjects (p>0.05).
  
  
  CONCLUSIONS
  Altered betaAPP isoforms is a high risk factor for Alzheimer's disease, although it has no influence on the time of onset of the disease.
authors.affiliation: Laboratorio de Desarrollo-Envejecimiento, Enfermedades Neurodegenerativas, División de Neurociencias, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México. vjzahiiir@yahoo.com
authors.forename: V J
authors.initials: VJ
authors.lastname: Sánchez-González
authors.forename: G G
authors.initials: GG
authors.lastname: Ortiz
authors.forename: P
authors.initials: P
authors.lastname: Gallegos-Arreola
authors.forename: M A
authors.initials: MA
authors.lastname: Macías-Islas
authors.forename: E D
authors.initials: ED
authors.lastname: Arias-Merino
authors.forename: V
authors.initials: V
authors.lastname: Loera-Castañeda
authors.forename: E
authors.initials: E
authors.lastname: Martínez-Cano
authors.forename: I E
authors.initials: IE
authors.lastname: Velázquez-Brizuela
authors.forename: S A
authors.initials: SA
authors.lastname: Rosales-Corral
authors.forename: C R
authors.initials: CR
authors.lastname: Curiel-Ortega
authors.forename: F
authors.initials: F
authors.lastname: Pacheco-Moisés
authors.forename: J J
authors.initials: JJ
authors.lastname: García
mesh_terms: D000368:Aged; D000483:Alleles; D000544:Alzheimer Disease / Q000175:diagnosis*; D016229:Amyloid beta-Peptides / Q000097:blood; D016564:Amyloid beta-Protein Precursor / Q000097:blood*; D001057:Apolipoproteins E / Q000235:genetics; D015153:Blotting, Western; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020033:Protein Isoforms / Q000097:blood
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D001057:Apolipoproteins E; D020033:Protein Isoforms