Application of monolithic column in quantification of gliclazide in human plasma by liquid chromatography.
A simple, rapid and sensitive isocratic reversed phase HPLC method with UV detection using a monolithic column has been developed and validated for the determination of gliclazide in human plasma. The assay enables the measurement of gliclazide for therapeutic drug monitoring with a minimum quantification limit of 10ngml(-1). The method involves simple, one-step extraction procedure and analytical recovery was complete. The separation was carried out in reversed-phase conditions using a Chromolith Performance (RP-18e, 100mmx4.6mm) column with an isocratic mobile phase consisting of 0.01M disodium hydrogen phosphate buffer-acetonitrile (52:48, v/v) adjusted to pH 4.0. The wavelength was set at 230nm. The calibration curve was linear over the concentration range 10-5000ngml(-1). The coefficients of variation for inter-day and intra-day assay were found to be less than 6.0%.
Foroutan
S M
SM
Department of Pharmaceutics, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran. mforoutan@excite.com
Zarghi
A
A
Shafaati
A
A
Khoddam
A
A
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D016903:Drug Monitoring; D005907:Gliclazide / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D007004:Hypoglycemic Agents; D005907:Gliclazide