Electrochemically amplified detection for lipopolysaccharide using ferrocenylboronic acid.
A novel electrochemical technique for lipopolysaccharide (LPS) detection has been developed using a combination of ferrocenylboronic acid derivatives and an enzyme-modified electrode. The enzyme-modified electrode was constructed from a gold electrode modified with a bovine serum albumin membrane containing diaphorase. Ferrocenylboronic acid derivatives are oxidized on the electrode, and then regenerated by a diaphorase-catalyzed reaction in the presence of NADH. The consumption/regeneration cycle for ferrocenylboronic acid derivatives resulted in a chemically amplified current response. The current response for ferrocenylboronic acid derivatives decreased in association with its complexation with glycosyl units of LPS, and this current decrease caused by LPS was also amplified by the recycling process. On the other hand, the addition of a monosaccharide such as D-mannose or D-galactose induced no response at the same LPS concentration. The enzyme membrane immobilized on the electrode plays an important role in selectivity as well as chemical amplification. In addition, the enzyme-modified electrode exhibited a rapid response of 5 min for LPS, which is much faster than the currently used method. The detection limit of LPS from Escherichia coli O127:B8 was as low as 50 ng ml-1.
Kato
Dai
D
JST-CREST, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan.
Iijima
Seiichiro
S
Kurita
Ryoji
R
Sato
Yukari
Y
Jia
Jianbo
J
Yabuki
Soichi
S
Mizutani
Fumio
F
Niwa
Osamu
O
D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds; D008070:Lipopolysaccharides / Q000032:analysis*
D005296:Ferrous Compounds; D008070:Lipopolysaccharides; C420283:ferrocenyl boronate