Molecular characterization of a potato MAP kinase transcriptionally regulated by multiple environmental stresses.
The MAPK cascade is an evolutionary conserved signaling pathway that links external stimuli with cellular responses. Using polymerase chain reaction (PCR), a DNA fragment corresponding to a Solanum tuberosum MAPK, StMPK1, was isolated. StMPK1 amino acid sequence displayed over 90% identity with tomato MPK1 (LeMPK1) and tobacco SIPK. Southern blot analysis indicated that the gene encoding StMPK1 is present in a single copy in the potato genome. StMPK1 mRNA levels differentially accumulated in potato tuber in response to wounding and to wounding plus Fusarium solani f. sp. eumartii. Transcript accumulation after infection was transient and started earlier than what was observed in wounded tubers. StMPK1 mRNA levels also increased in potato tuber after 24 h of treatment with jasmonic acid (JA) and abscicic acid (ABA), but not in response to ethylene or salicylic acid. In addition, StMPK1 transcript levels increased after a heat-shock treatment at 42 degrees C. The results suggest that StMPK1 may participate in the cellular responses against multiple environmental stimuli in potato tubers.
Blanco
Flavio Antonio
FA
Instituto de Investigaciones Biológicas-Departamento de Biología, Universidad Nacional de Mar del Plata, Funes 3250, CC 1245, 7600 Mar del Plata, Argentina. fablanco@biol.unlp.edu.ar
Zanetti
María Eugenia
ME
Casalongué
Claudia Anahí
CA
Daleo
Gustavo Raúl
GR
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D003517:Cyclopentanes / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D005670:Fusarium; D018506:Gene Expression Regulation, Plant; D018869:Heat-Shock Response; D006358:Hot Temperature; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D054883:Oxylipins; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D035281:Plant Tubers / Q000187:drug effects / Q000201:enzymology* / Q000382:microbiology; D012333:RNA, Messenger / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000187:drug effects / Q000201:enzymology* / Q000382:microbiology
D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010940:Plant Proteins; D012333:RNA, Messenger; C011006:jasmonic acid; D000040:Abscisic Acid; C036216:ethylene; D020928:Mitogen-Activated Protein Kinases; D020156:Salicylic Acid