Parallel artificial membrane permeability assay: a new membrane for the fast prediction of passive human skin permeability.
This work was devoted to the search for new artificial membranes allowing a rapid evaluation of passive human skin permeation of compounds with a parallel artificial membrane permeability assay (PAMPA). Effective permeability coefficients (Pe) determined for a set of compounds using the PAMPA technique with isopropyl myristate (IPM) and silicone oil, alone or in mixture, were compared to the corresponding human skin permeability coefficient values (Kp). A good correlation between Pe and Kp was found for compounds tested through a membrane consisting of 70% silicone and 30% IPM. Moreover, positive correlation between the membrane retention of compounds and stratum corneum/water partition coefficients (PSC) was established. These results showed that this new artificial membrane, defined as PAMPA-skin, is able to mimic the main barrier properties of human stratum corneum and can be used for the fast prediction of passive human skin permeability coefficients.
Ottaviani
Giorgio
G
LCT-Pharmacochimie, Section des Sciences Pharmaceutiques, Université de Genève, Quai Ernest-Ansermet 30, CH-1211, Genève 4, Switzerland.
Martel
Sophie
S
Carrupt
Pierre-Alain
PA
D020003:1-Octanol / Q000737:chemistry; D004058:Diffusion; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D009226:Myristates / Q000737:chemistry*; D010539:Permeability; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D014867:Water / Q000737:chemistry
D004129:Dimethylpolysiloxanes; D008567:Membranes, Artificial; D009226:Myristates; D014867:Water; C008205:isopropyl myristate; D020003:1-Octanol