Mobility of mass-reared diapaused and nondiapaused Cydia pomonella (Lepidoptera: Tortricidae): effect of mating status and treatment with gamma radiation.
Mass-reared insects may differ in their behavioral traits depending on whether they have undergone diapause. We studied the mobility of mass-reared diapaused and nondiapaused Cydia pomonella (L.) with a focus on understanding the effect of mating status and treatment with gamma radiation as these insects are destined for use in an areawide program that uses the sterile insect technique (SIT). Actograph-measured mobility was assessed one gender at a time for 4 h during which the photoperiod transitioned from day to night. We tested 20-30 individuals per treatment. For experiments on the effect of mating status, we used 24-48-h-old adults (diapause [D]-virgin, D-mated, standard [N]-virgin, and N-mated), which is the typical age class that is released in the SIT program. Diapaused females were significantly more mobile than females reared through standard production, whereas no differences were detected in male mobility because of rearing strategy. Mated females were significantly more mobile than virgin females, whereas no difference in mobility because of mating status was detected for males. Mated females were significantly more mobile than mated males. In contrast, virgin females were significantly less mobile than virgin males. For experiments on the effect of treatment with gamma radiation, adults from all rearing strategies and treatments (D-0Gy, D-100Gy, D-250Gy, N-0Gy, N-100Gy, and N-250Gy), were tested simultaneously. Adult males were tested at two different constant temperatures (25 and 20 degrees C), whereas adult females were tested only at 25 degrees C. For standard-reared adults, we found a significant linear decrease in mobility as the radiation dose increased from 0 to 250 Gy. In contrast, the relationship between mobility and dose of radiation was quadratic for diapaused males and absent for diapaused females.
Bloem
Stephanie
S
Institute of Plant Sciences/Applied Entomology, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland.
Carpenter
James E
JE
Dorn
Silvia
S
D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D008297:Male; D009036:Moths / Q000502:physiology* / Q000528:radiation effects; D009043:Motor Activity / Q000502:physiology* / Q000528:radiation effects; D010572:Pest Control, Biological / Q000379:methods; D017440:Photoperiod; D012726:Sexual Behavior, Animal