title: The influence of sex and social isolation housing on pre- and postsynaptic 5-HT1A receptors.
abstract: Serotonergic (5-HT) receptors are crucial for different brain functions and play an important role in several pathological conditions. We analysed [3H]8-OH-DPAT-specific binding to 5-HT1A receptors in male and female mice after group or isolation housing by in vitro autoradiography (n = 6 per group). Females displayed higher postsynaptic 5-HT1A receptor binding compared to males, especially in the cortex. In contrast, lower [3H]8-OH-DPAT-specific binding was found in the female hippocampus. No sex difference was seen for the somatodendritic 5-HT1A autoreceptor. Sex differences in postsynaptic 5-HT1A receptor binding should be relevant to behavioural sex differences, especially in locomotor activity and hippocampus-dependent behaviours. Six weeks isolation housing caused an increase in 5-HT1A receptor binding in most of the brain regions analysed and was more pronounced in males. In isolated males, the increases were detected in the CA1 field of the hippocampus (+16.8%), in the septum (+76.8%), in the cortical amygdala (+24.6%), in the periaqueductal gray (+67.2%) and in the different cortical regions analysed (+61.8-81.4%). [3H]8-OH-DPAT-specific binding increased significantly in the dentate gyrus (+47.1%), the supramammillary nucleus (+31.2%) and in the ventromedial hypothalamus (+34.4%) of isolated females. Sex-dependent isolation-induced alterations in [3H]8-OH-DPAT-specific binding were also found in the raphe nuclei. Isolation-induced increases in 5-HT1A receptor binding could be relevant to the behavioural disinhibition with heightened arousal, impulsivity and activity often observed in isolates. The male-specific alterations in the corticolimbic system as well as in the midbrain could be crucial for isolation-induced aggression.
authors.affiliation: AG Neurobiologie, Klinik für Psychiatrie, Universitätsklinikum der TU Dresden, Fetscherstrasse 74, 01307 Dresden, Germany. Lydia.Schiller@mailbox.tu-dresden.de
authors.forename: Lydia
authors.initials: L
authors.lastname: Schiller
authors.forename: Monika
authors.initials: M
authors.lastname: Jähkel
authors.forename: Jochen
authors.initials: J
authors.lastname: Oehler
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry / Q000187:drug effects*; D003627:Data Interpretation, Statistical; D003712:Dendrites / Q000378:metabolism; D005260:Female; D008297:Male; D051379:Mice; D044282:Receptor, Serotonin, 5-HT1A / Q000502:physiology*; D017661:Receptors, Presynaptic / Q000502:physiology*; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D012727:Sex Characteristics; D012934:Social Isolation; D013569:Synapses / Q000502:physiology*
chemical_list: D017661:Receptors, Presynaptic; D017366:Serotonin Receptor Agonists; D044282:Receptor, Serotonin, 5-HT1A; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin