title: Effects of growth factors on cell migration and alkaline phosphatase release.
abstract: Segmental defects in long bones pose a clinical challenge in orthopaedic surgery. These defects often require multiple surgeries to achieve the desired bridging and union. Bone morphogenetic protein 2 (BMP-2) has been used extensively to facilitate regeneration in these large defects. The two important roles that BMP-2 has to play in large defect regeneration are chemotaxis to bone cells to migrate and induction of bone matrix production. Experiments were designed to study the effect of BMP-2 on alkaline phosphatase (ALP) production in cells that have migrated through a porous membrane in a cell migration chamber under the influence of culture media supplemented with BMP-2. Two cell lines, UMR cells and MC3T3-E1 cells, were used. A modified Boyden chamber with 8 microm membrane filter was used. Cell migration under the influence of BMP-2 concentration of 1, 10, 100, and 1000 ng/ml was studied. In both cells, the highest chemotactic effect was observed at 10 ng/ml. The ALP activity in the migrated cell was then characterized. A pattern of increase in ALP activity with increased BMP-2 dose was observed in the migrated cells.
authors.affiliation: Department of Biomedical Engineering, Indiana University-Purdue University Indianapolis, 1120 South Drive, Rm. 146, Indianapolis, IN 46202, USA.
authors.forename: Krista N
authors.initials: KN
authors.lastname: Gonnerman
authors.forename: Lauren S
authors.initials: LS
authors.lastname: Brown
authors.forename: Tien-Min G
authors.initials: TM
authors.lastname: Chu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001857:Bone Matrix / Q000187:drug effects / Q000502:physiology*; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D007335:Insulin-Like Growth Factor II; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D011506:Proteins / Q000008:administration & dosage; D016212:Transforming Growth Factor beta / Q000008:administration & dosage*
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; C035170:IGF2 protein, human; D011506:Proteins; D016212:Transforming Growth Factor beta; D007335:Insulin-Like Growth Factor II; D000469:Alkaline Phosphatase