Growth characteristics of fusants by protoplast fusion between the thermotolerant yeast, Kluyveromyces marxianus, and the starch-assimilating yeast, Schwanniomyces occidentalis.
Intergeneric fusants were obtained by protoplast fusion between the thermotolerant yeast, Kluyveromyces marxianus, and the starch-assimilating yeast, Schwanniomyces occidentalis. Two thermotolerant fusants growing at 40 degrees C were screened on the medium containing soluble starch. These fusants showed weak growth in a soluble starch medium and the production of a little amylase. The carbon source assimilation and the chromosome composition of the fusants were similar to those of the K. marxianus parent. However, a chromosomal difference from K. marxianus was recognized in the fusants. These results show the possibility that the fusants are amylase-producing strains rearranged from K. marxianus.
Leelavatcharamas
Vichai
V
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand.
Boonyakamola
Anantaphathra
A
Kishida
Masao
M
Kawasaki
Haruhiko
H
D015825:Chromosomes, Fungal; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D011523:Protoplasts; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D013213:Starch / Q000378:metabolism*; D013696:Temperature; D000516:alpha-Amylases / Q000096:biosynthesis
D013213:Starch; D000516:alpha-Amylases