title: Improvement in yield and purity of a recombinant malaria vaccine candidate based on the receptor-binding domain of Plasmodium vivax Duffy binding protein by codon optimization.
abstract: A recombinant blood-stage vaccine for Plasmodium vivax malaria based on the functional receptor-binding domain of PvDBP (PvRII) has been developed. A synthetic gene coding for PvRII was expressed in Escherichia coli using codon optimization. Expression level of recombinant PvRII was 10% of the total cellular proteins. Truncated PvRII products, seen when the native PvRII gene was expressed, were absent in case of synthetic gene.
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, India. shams@icgeb.res.in
authors.forename: Syed Shams
authors.initials: SS
authors.lastname: Yazdani
authors.forename: Ahmad Rushdi
authors.initials: AR
authors.lastname: Shakri
authors.forename: Priyabrata
authors.initials: P
authors.lastname: Pattnaik
authors.forename: M Moshahid A
authors.initials: MM
authors.lastname: Rizvi
authors.forename: Chetan E
authors.initials: CE
authors.lastname: Chitnis
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D024861:Genetic Enhancement / Q000379:methods*; D017780:Malaria Vaccines / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D010966:Plasmodium vivax / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: D000953:Antigens, Protozoan; D003062:Codon; C066001:Duffy antigen binding protein, Plasmodium; D017780:Malaria Vaccines; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins