Granulocyte-macrophage colony-stimulating-factor increases interferon-tau protein secretion in bovine trophectoderm cells.
OBJECTIVE
Uterine-derived factors are required for optimal conceptus development and secretion of the maternal recognition of pregnancy factor, interferon-tau (IFN-tau). Identifying these factors may lead to the development of schemes for increasing pregnancy success in cattle.


METHODS
The objectives were to examine the effects of granulocyte-macrophage colony-stimulating-factor (GM-CSF) on trophectoderm proliferation rates and IFN-tau production, and verify the appropriateness of using an in vitro model of bovine trophectoderm (CT-1 cell).


RESULTS
Rate of [(3)H]-thymidine incorporation into DNA was increased by supplementation of CT-1 medium with 10 or 100 ng/mL porcine (po) GM-CSF. GM-CSF supplementation to CT-1 medium also increased IFN-tau secretion. When results were normalized to account for number of CT-1 cells, 10 and 100 ng/mL poGM-CSF increased antiviral activity and IFN-tau concentrations (using an IFN-tau-specific enzyme-linked immunosorbent assay) in CT-1 conditioned medium compared with controls.


CONCLUSIONS
These findings indicate that GM-CSF increases proliferation and IFN-tau production in bovine trophectoderm.
Michael
Donna D
DD
Department of Dairy & Animal Science, The Pennsylvania State University, University Park, 16811, USA.
Wagner
Sara K
SK
Ocón
Olga M
OM
Talbot
Neil C
NC
Rooke
John A
JA
Ealy
Alan D
AD
D000818:Animals; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D007370:Interferon Type I / Q000378:metabolism*; D011257:Pregnancy Proteins / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D007370:Interferon Type I; D011257:Pregnancy Proteins; C020947:interferon tau; D016178:Granulocyte-Macrophage Colony-Stimulating Factor