Dependence of Vegetative Recombination Among Haemophilus influenzae Bacteriophage on the Host Cell.
Vegetative recombination of temperature-sensitive mutants of Haemophilus influenzae phage HP1 cl was measured in wild-type H. influenzae strain Rd and in strain DB117, an ultraviolet-sensitive, transformation-defective mutant of the Rd strain. Recombinants are formed with low frequency in wild-type cells, but no recombination was detectable in DB117. It is concluded that these phage make use of the host cell enzymes for vegetative recombination. Lysogenization readily takes place in both strains.
Boling
M E
ME
Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830.
Setlow
J K
JK