Synthesis and DNA cleavage activities of mononuclear macrocyclic polyamine zinc(II), copper(II), cobalt(II) complexes which linked with uracil.
Mononuclear macrocyclic polyamine zinc(II), copper(II), cobalt(II) complexes, which could attach to peptide nucleic acid (PNA), were synthesized as DNA cleavage agents. The structures of these new mononuclear complexes were identified by MS and (1)H NMR spectroscopy. The catalytic activities on DNA cleavage of these mononuclear complexes with different central metals were subsequently studied, which showed that copper complex was better catalyst in the DNA cleavage process than zinc and cobalt complexes. The effects of reaction time, concentration of complexes were also investigated. The results indicated that the copper(II) complexes could catalyze the cleavage of supercoiled DNA (pUC 19 plasmid DNA) (Form I) under physiological conditions to produce selectively nicked DNA (Form II, no Form III produced) with high yields. The mechanism of the cleavage process was also studied.
Wang
Xiao-Yan
XY
Department of Chemistry, Key Laboratory of Green Chemistry and Technology (Ministry of Education), Sichuan University, Chengdu, 610064, PR China.
Zhang
Ji
J
Li
Kun
K
Jiang
Ning
N
Chen
Shan-Yong
SY
Lin
Hong-Hui
HH
Huang
Yu
Y
Ma
Li-Jian
LJ
Yu
Xiao-Qi
XQ
D003035:Cobalt; D003300:Copper; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000494:pharmacology*; D010957:Plasmids / Q000737:chemistry; D011073:Polyamines / Q000138:chemical synthesis* / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry; D014498:Uracil / Q000737:chemistry*; D017967:Zinc Compounds / Q000138:chemical synthesis* / Q000494:pharmacology*
D007202:Indicators and Reagents; D009942:Organometallic Compounds; D011073:Polyamines; D017392:Thiobarbituric Acid Reactive Substances; D017967:Zinc Compounds; D003035:Cobalt; D014498:Uracil; D003300:Copper; D004247:DNA