title: Effects of ephedrae herba on melanogenesis and gene expression profiles using cDNA microarray in B16 melanocytes.
abstract: Ephedrae Herba (EH) is obtained by drying the stems of Ephedra sinica Stapf (family Ephedraceae). EH has been used clinically to treat colds and to reduce edema in skin. The effects of EH on melanogenesis were studied in B16 murine melanocytes. The tyrosinase activity and melanin content were measured after incubation with EH. Both reverse transcription-polymerase chain reaction and cDNA microarray analysis were used to study the mechanism of EH action. EH decreased tyrosinase activity and melanin content in a dose-dependent manner. EH decreased microphthalmia-associated transcription factor (MITF) and tyrosinase gene expression. These effects were controlled by MITF-mediated regulation of tyrosinase gene expression. EH also altered the expression of about 100 other genes. These results suggest that EH may be used clinically to treat freckles and liver spots.
authors.affiliation: College of Oriental Medicine, Semyung University, Jechon, South Korea.
authors.forename: Hyeong Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Yeong Do
authors.initials: YD
authors.lastname: Cho
authors.forename: Kang-Hyun
authors.initials: KH
authors.lastname: Leem
authors.forename: Dong-Nyung
authors.initials: DN
authors.lastname: Lee
authors.forename: Ee-Hwa
authors.initials: EH
authors.lastname: Kim
authors.forename: Myung-Gyou
authors.initials: MG
authors.lastname: Kim
authors.forename: Dae-Keun
authors.initials: DK
authors.lastname: Kim
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
authors.forename: Yungmin
authors.initials: Y
authors.lastname: Boo
authors.forename: Je-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D029790:Ephedra sinica; D015870:Gene Expression / Q000187:drug effects; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects*; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D010936:Plant Extracts; D014442:Monophenol Monooxygenase