[Technique of detection of hepatitis C core antigen used in safety blood transfusion].
This study was purposed to investigate the feasibility to screen donor with HCV infection by means of HCV-cAg ELISA. The first and repeat assays were performed for detection of serum anti-HCV in 8677 donor's serum specimens from January 2003 to December 2005. All serum anti-HCV specimens with positive anti-HCV from first and repeat assays were finally identified by using HCV-cAg ELISA and HCV RT-PCR methods. The results showed that only 5 serum specimens were positive anti-HCV by HCV-cAg ELISA identification in 29 specimens including 15 specimens with positive ant-HCV in first assays and 14 specimens with positive anti-HCV in repeat assays, the positive rate detected by HCV cAg ELISA was 17.24%. 5 serum specimens were positive anti-HCV by HCV RT-PCR detection also in 29 specimens mentioned above, the positive rate detected by HCV RT-PCR was 17.24% too. It is concluded that sensitivity of HCVcAg ELISA is similar to HCV RT-PCR and may be useful for the early diagnosis of hepatitic C or used as a reliable method to screen donor with HCV infection in blood transfusion medicine.
Yao
Ren-Nan
RN
Department of Transfusion, 97th Hospital of PLA, Xuzhou 221004, China. yaorn97@sohu.com
Zhang
Jian-Hui
JH
Huang
Xiao-Jing
XJ
Yang
Qin
Q
Cao
Qin
Q
Jiang
Xu-Cheng
XC
D001782:Blood Donors; D001803:Blood Transfusion; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016174:Hepacivirus / Q000302:isolation & purification*; D018936:Hepatitis C Antigens / Q000097:blood*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014758:Viral Core Proteins / Q000097:blood*
D018936:Hepatitis C Antigens; D014758:Viral Core Proteins