Building of an immunosensor: how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?
Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated.
Briand
Elisabeth
E
Laboratoire de Réactivité de Surface, UMR CNRS 7609, Université Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05, France.
Salmain
Michèle
M
Herry
Jean-Marie
JM
Perrot
Hubert
H
Compère
Chantal
C
Pradier
Claire-Marie
CM
D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013205:Staphylococcal Protein A / Q000032:analysis / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D000906:Antibodies; D003432:Cross-Linking Reagents; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D013438:Sulfhydryl Compounds