High-performance thin-layer chromatography densitometric method for simultaneous quantitation of phyllanthin, hypophyllanthin, gallic acid, and ellagic acid in Phyllanthus amarus.
Whole plant of Phyllanthus amarus Linn. is a reputed drug of the Indian systems of medicine that is used as hepatoprotective agent. A simple high-performance thin-layer chromatography (HPTLC) densitometric method has been developed for the simultaneous quantitation of phyllanthin, hypophyllanthin, gallic acid, and ellagic acid in the whole plant of P. amarus. They were found at levels of 0.37, 1.16, 0.36, and 0.17% (w/w), respectively. The method was validated for precision, repeatability, and accuracy. Instrumental precision was found to be 0.54, 0.93, 0.08, and 0.78% (coefficient of variation, CV); repeatability of the method was 1.01, 0.79, 0.98, and 1.06% (CV) for phyllanthin, hypophyllanthin, gallic acid, and ellagic acid, respectively. Accuracy of the method was determined by a recovery study conducted at 3 different levels, and the average recovery was found to be 99.09% for phyllanthin, 99.27% for hypophyllanthin, 98.69% for gallic acid, and 100.49% for ellagic acid. The proposed HPTLC method was found to be simple, precise, specific, sensitive, and accurate and can be used for routine quality control of raw material of P. amarus and formulations containing P. amarus. It also has the applicability in quantitating any of these marker compounds in other drugs.
Dhalwal
Kamlesh
K
B.V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Thaltej-Gandhinagar Hwy, Thaltej, Ahmedabad 380 054, Gujarat, India.
Biradar
Yogesh S
YS
Rajani
Mandapati
M
D000470:Alkaloids / Q000032:analysis* / Q000302:isolation & purification; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods*; D004610:Ellagic Acid / Q000032:analysis* / Q000302:isolation & purification; D005707:Gallic Acid / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification; D029803:Phyllanthus / Q000378:metabolism*; D010936:Plant Extracts / Q000032:analysis; D015203:Reproducibility of Results; D013997:Time Factors
D000470:Alkaloids; D017705:Lignans; D010936:Plant Extracts; D004610:Ellagic Acid; C416057:phyllanthine; C512682:hypophyllanthin; D005707:Gallic Acid