title: Recovery and characterization of a 30.7-kDa protein from Bacillus licheniformis associated with inhibitory activity against methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and Listeria monocytogenes.
abstract: Of 131 bacterial isolates from seaweed, a culture of Bacillus licheniformis produced a novel protein with antibacterial activity against methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and Listeria monocytogenes. The antibacterial activity was maximal in cultures prepared in Columbia broth containing pieces of synthetic polyurethane sponge and shaken at 210 to 230 rpm. Antibacterial activity was not found in cultures grown statically or with different speeds of rotary shaking. Reduced activity was apparent in supernatants prepared from marine 2216E broth and tryptone soya broth with or without 1% (wt/vol) sodium chloride. The antibacterial compound was sensitive to proteinase K, pronase, and trypsin, but was not affected by Tween-20, -40, -60, or -80, or alpha- or beta-amylase. Activity was not adversely affected by heating up to 40 degrees C or treatment at pH 5 to 14. The bioactive compound was determined to be associated with a protein of 30.7 kDa, which had homology to the YbdN protein of B. licheniformis ATCC 14580.
authors.affiliation: School of Life Sciences, John Muir Building, Heriot-Watt University, Riccarton, Edinburgh, EH14 4AS, Scotland, UK.
authors.forename: Mamdoh T
authors.initials: MT
authors.lastname: Jamal
authors.forename: Peter C
authors.initials: PC
authors.lastname: Morris
authors.forename: Rasmus
authors.initials: R
authors.lastname: Hansen
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Jamieson
authors.forename: J Grant
authors.initials: JG
authors.lastname: Burgess
authors.forename: Brian
authors.initials: B
authors.lastname: Austin
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001407:Bacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism / Q000494:pharmacology*; D016983:Enterococcus / Q000187:drug effects*; D008089:Listeria monocytogenes / Q000187:drug effects*; D016106:Methicillin Resistance; D013211:Staphylococcus aureus / Q000187:drug effects*; D020713:Vancomycin Resistance
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins