Identification of a dithiol-dependent nucleoside triphosphate hydrolase in Sarcocystis neurona.
A putative nucleoside triphosphate hydrolase (NTPase) gene was identified in a database of expressed sequence tags (ESTs) from the apicomplexan parasite Sarcocystis neurona. Analysis of culture-derived S. neurona merozoites demonstrated a dithiol-dependent NTPase activity, consistent with the presence of a homologue to the TgNTPases of Toxoplasma gondii. A complete cDNA was obtained for the S. neurona gene and the predicted amino acid sequence shared 38% identity with the two TgNTPase isoforms from T. gondii. Based on the obvious homology, the S. neurona protein was designated SnNTP1. The SnNTP1 cDNA encodes a polypeptide of 714 amino acids with a predicted 22-residue signal peptide and an estimated mature molecular mass of 70kDa. Southern blot analysis of the SnNTP1 locus revealed that the gene exists as a single copy in the S. neurona genome, unlike the multiple gene copies that have been observed in T. gondii and Neospora caninum. Analyses of the SnNTP1 protein demonstrated that it is soluble and secreted into the culture medium by extracellular merozoites. Surprisingly, indirect immunofluorescence analysis of intracellular S. neurona revealed apical localisation of SnNTP1 and temporal expression characteristics that are comparable with the microneme protein SnMIC10. The absence of SnNTP1 during much of endopolygeny implies that this protein does not serve a function during intracellular growth and development of S. neurona schizonts. Instead, SnNTP1 may play a role in events that occur during or proximal to merozoite egress from and/or invasion into cells.
Zhang
Deqing
D
Department of Veterinary Sciences, University of Kentucky, Gluck Equine Research Center, Lexington, KY 40546-0099, USA.
Gaji
Rajshekhar Y
RY
Howe
Daniel K
DK
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D019084:Fluorescent Antibody Technique, Indirect; D017125:Genes, Protozoan; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D018688:Neospora / Q000235:genetics; D043583:Nucleoside-Triphosphatase / Q000032:analysis / Q000235:genetics*; D010274:Parasitology / Q000379:methods; D012522:Sarcocystis / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology; D014050:Toluene / Q000031:analogs & derivatives; D014122:Toxoplasma / Q000235:genetics; D014774:Virulence / Q000235:genetics
D014050:Toluene; D043583:Nucleoside-Triphosphatase; C004848:dithiol