Neuropeptide Y in the olfactory microvillar cells.
This paper examines a possible role of microvillar cells in coordinating cell death and regeneration of olfactory epithelial neurons. The olfactory neuroepithelium of mammals is a highly dynamic organ. Olfactory neurons periodically degenerate by apoptosis and as a consequence of chemical or physical damage. To compensate for this loss of cells, the olfactory epithelium maintains a lifelong ability to regenerate from a pool of resident multipotent stem cells. To assure functional continuity and histological integrity of the olfactory epithelium over a period of many decades, apoptosis and regeneration require to be precisely coordinated. Among the factors that have been implicated in mediating this regulation is the neuropeptide Y (NPY). Knockout mice that lack functional expression of this neurogenic peptide show defects in embryonic development of the olfactory epithelium and in its ability to regenerate in the adult. Here we show that, in postnatal olfactory epithelia, NPY is exclusively expressed by a specific population of microvillar cells. We previously characterized these cells as a novel type of putative chemosensory cells, which are provided with a phosphatidyl-inositol-mediated signal transduction cascade. Our findings allow for the first time to suggest that microvillar cells are involved in connecting apoptosis to neuronal regeneration by stimulus-induced release of NPY.
Montani
Giorgia
G
Department of Neuroscience, University of Parma, Via Volturno, 39, I-43100, Parma, Italy.
Tonelli
Simone
S
Elsaesser
Rebecca
R
Paysan
Jacques
J
Tirindelli
Roberto
R
D000818:Animals; D019771:Axotomy; D015220:Calcium Channels / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008871:Microvilli / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism*; D009831:Olfactory Mucosa / Q000294:innervation / Q000378:metabolism* / Q000648:ultrastructure; D054799:Phospholipase C beta; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007527:Isoenzymes; D009478:Neuropeptide Y; D018160:Receptors, Cytoplasmic and Nuclear; D010738:Type C Phospholipases; D054799:Phospholipase C beta