mRNA expression of MAGE-A3 gene in leukemia cells.
Leukemia-associated antigens such as proteins encoded by MAGE genes might provide tools for immunotherapy of leukemia. Positive and negative results of MAGE-A gene expression in hematological malignancies have been reported. This led us to study MAGE-A gene expression in human leukemias using RT-PCR. Among 115 leukemias from various subtypes, 14/34 (41.17%) AML were positive for one of the three genes analyzed (MAGE-A1 1/32; MAGE-A3 10/32; MAGE-B2 3/12). Expression was also detected in 23/76 (30.26%) B-cell ALL patients (MAGE-A1 2/53; MAGE-A3 20/53; MAGE-B2 1/32). One of these patients expressed both MAGE-A1 (weak signal) and -A3 (strong signal) genes. Other patient with CML were positive for MAGE-B2 (1/5, 20%). MAGE-A3 expression data were corroborated by real time RT-PCR through determination of MAGE-A3 transcript levels. We concluded that the MAGE-A3 gene is expressed at the mRNA level in a proportion of human leukemias.
Martínez
A
A
Laboratorio de Biología Molecular, Servicio de Hematología, Hospital General de México, Dr. Balmis 148, Col. Doctores, 06726 México City D.F., Mexico.
Olarte
I
I
Mergold
M A
MA
Gutiérrez
M
M
Rozen
E
E
Collazo
J
J
Amancio-Chassin
O
O
Ordóñez
R M
RM
Montesinos
J J
JJ
Mayani
H
H
McCurdy
D K
DK
Ostrosky-Wegman
P
P
Garrido-Guerrero
E
E
Miranda
E I
EI
D000328:Adult; D000951:Antigens, Neoplasm / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D015470:Leukemia, Myeloid, Acute / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D000951:Antigens, Neoplasm; D017931:DNA Primers; C072013:MAGEA3 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger