Characterization of arylsulfatase formed by derepressed synthesis in Citrobacter braakii.
Arylsulfatase activity was detected in a bacterial strain, Citrobacter braakii 69-b, isolated from soil by enrichment cultivation using porcine gastric mucin. The production of arylsulfatase was derepressed markedly in a synthetic medium by the addition of tyramine. The purified enzyme hydrolyzed 4-nitrophenyl sulfate, 4-nitrocatechol sulfate, and 3-indoxyl sulfate, and was classified as type I arylsulfatase.
Miura
Toyokazu
T
Department of Biotechnology, Faculty of Engineering, Tottori University.
Okamoto
Kenji
K
Yanase
Hideshi
H
D001192:Arylsulfatases / Q000096:biosynthesis / Q000378:metabolism*; D002954:Citrobacter / Q000201:enzymology*; D013379:Substrate Specificity; D014439:Tyramine / Q000378:metabolism
D001192:Arylsulfatases; D014439:Tyramine