[Effect of novel human chemokine-like factor superfamily 8 on proliferation and EGFR expression of tumor cells].
OBJECTIVE
To investigate the effect of chemokine-like factor superfamily 8 (CKLFSF8) on proliferation and epidermal growth factor receptor (EGFR) expression of tumor cells.


METHODS
Expression of CKLFSF8 mRNA in BGC823 and K562 cells was assayed by RT-PCR. Then target gene was transfected into tumor cells by lipid method, and cell growth was observed by inverted microscope. Cell proliferation was determined by MTT assay and expression of EGFR on tumor cells was determined by immunocytochemical technique.


RESULTS
CKLFSF8 was expressed in the two tumor cells. After transfection, cell proliferation was inhibited (P<0.05) and the expression of EGFR of tumor cells was also discovered to be inhibited (P<0.05).


CONCLUSIONS
The finding shows that cell proliferation and expression of EGFR of tumor cells can be inhibited by transfection of CKLFSF8. The new chemokine may has a bright prospect in the treatment of tumor.
Huang
Yu-min
YM
Department of Microbiology and Immunology, Basic Medical School, Zhengzhou University, Zhengzhou 450052, China. huang-yumin@126.com
Du
Ying
Y
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018925:Chemokines / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D062792:MARVEL Domain-Containing Proteins; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D014162:Transfection
C507076:CMTM8 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; C512478:EGFR protein, human; D066246:ErbB Receptors; D004262:DNA Restriction Enzymes