title: Immobilization of proteins on agarose beads, monitored in real time by bead injection spectroscopy.
abstract: A novel approach to real-time monitoring of protein immobilization resulted in the surprising finding that current immobilization protocols are far from optimized.
authors.affiliation: Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA.
authors.forename: Jaromir
authors.initials: J
authors.lastname: Ruzicka
authors.forename: Andrea D
authors.initials: AD
authors.lastname: Carroll
authors.forename: Ilkka
authors.initials: I
authors.lastname: Lähdesmäki
mesh_terms: D017022:Flow Injection Analysis / Q000379:methods; D008832:Microchemistry / Q000379:methods*; D008863:Microspheres; D011506:Proteins / Q000737:chemistry*; D012685:Sepharose; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D011506:Proteins; D012685:Sepharose