title: Analysis of clinical manifestations, mutant gene and encoded protein in two Chinese MYH9-related disease families.
abstract: BACKGROUND
  MYH9-related disease is a rare autosomal dominant disorder characterized by the triad of giant platelet, thrombocytopenia and inclusion bodies in neutrophil. In recent years, much progress has been made in the investigation of its clinical feature and pathogenesis.
  
  
  METHODS
  Clinical manifestations were analyzed in two Chinese MYH9-related disease families. Polymerase chain reaction (PCR), DNA sequencing and CpoI restrictive endonuclease map analysis were used to identify spot mutation in nonmuscle myosin heavy chain 9 (MYH9) gene. Indirect immunofluence combined propidium iodine (PI) nuclei count-staining technology was applied to probe nonmuscle myosin heavy chain IIA (NMMHC-A) in MYH9-related disease neutrophils and platelets. Western blot was undergone to examine the expression of NMMHC-A in MYH9-related disease patients.
  
  
  RESULTS
  All of the patients manifested with the typical triad, mild to moderate bleeding tendency were their common clinical feature, some patients were accompanied by renal lesion. G5521A mutation in MYH9 gene was identified in both families. Spindle-like inclusions with yellow fluorescence in MYH9-related disease neutrophils were clearly revealed by indirect immunofluence combined PI nuclei count-staining technology, which matched very well with the inclusions, detected by Wright-Giemsa's stain. An upregulation of NMMHC-A in MYH9-related disease neutrophils was observed by Western blotting analysis.
  
  
  CONCLUSIONS
  Mutation of MYH9 gene exists in cases of Chinese MYH9-related disease. In the two families, the point mutation was located in exon 38(G5521A), and the transference rule of the MYH9 gene mutation is corresponding with clinical phenotype distribution. Indirect immunofluorescence combining with PI nuclei staining technology is sensitive and more specific than Wright-Giemsa's staining in detecting MYH9-related disease inclusions, with which we might easily distinguish MYH9-related disease inclusions from infection-associated inclusions. The expression of the NMMHC-A in MYH9-related disease neutrophils was upregulated than normal control.
authors.affiliation: Division of Hematology/Institute of Molecular Hematology, the Second Xiang-ya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yi
authors.forename: Guang
authors.initials: G
authors.lastname: Sen Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Zhu
authors.initials: Z
authors.lastname: San Ling
authors.forename: Xiu
authors.initials: X
authors.lastname: Ru Shao
authors.forename: Jia
authors.initials: J
authors.lastname: Zeng Li
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000473:pathology; D015153:Blotting, Western; D002681:China; D004252:DNA Mutational Analysis; D005091:Exons; D005192:Family Health; D005260:Female; D006801:Humans; D008297:Male; D020409:Molecular Motor Proteins / Q000235:genetics* / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics* / Q000378:metabolism; D009504:Neutrophils / Q000473:pathology; D010375:Pedigree; D010641:Phenotype; D017354:Point Mutation; D016891:Polycystic Kidney, Autosomal Dominant / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D015854:Up-Regulation
chemical_list: C103814:MYH9 protein, human; D020409:Molecular Motor Proteins; D018995:Myosin Heavy Chains