title: Mechanism of drug resistance in clonally related clinical isolates of Vibrio fluvialis isolated in Kolkata, India.
abstract: The molecular mechanisms of drug resistance in 19 strains of Vibrio fluvialis isolated from 1998 to 2002 in Kolkata, India, were investigated. Class 1 integrons were detected in eight strains, and four strains were found to carry SXT integrases. In the presence of carbonyl cyanide m-chlorophenylhydrazone or reserpine, all nalidixic acid- and ciprofloxacin-resistant strains became sensitive, suggesting that drug efflux plays a major role in quinolone resistance in V. fluvialis. It was further seen that strains which had MICs of > 25 microg/ml for nalidixic acid had a sense mutation (Ser to Ile) at position 83 of the quinolone resistance-determining region of gyrA. All except one of the integron- and SXT integrase-bearing strains belonged to the same ribotype.
authors.affiliation: Institute of Microbial Technology, Molecular Biology Division, Sector 39A, Chandigarh 160036, India. vijaya@imtech.res.in
authors.forename: Vijaya Bharathi
authors.initials: VB
authors.lastname: Srinivasan
authors.forename: Rupinder Kaur
authors.initials: RK
authors.lastname: Virk
authors.forename: Amita
authors.initials: A
authors.lastname: Kaundal
authors.forename: Rupa
authors.initials: R
authors.lastname: Chakraborty
authors.forename: Basabjit
authors.initials: B
authors.lastname: Datta
authors.forename: T
authors.initials: T
authors.lastname: Ramamurthy
authors.forename: Asish K
authors.initials: AK
authors.lastname: Mukhopadhyay
authors.forename: Amit
authors.initials: A
authors.lastname: Ghosh
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006760:Hospitalization; D006801:Humans; D007194:India; D019426:Integrases / Q000235:genetics; D032023:Integrons / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D010957:Plasmids; D015363:Quinolones / Q000494:pharmacology; D014733:Vibrio / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D014735:Vibrio Infections / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones; D009268:Nalidixic Acid; D019426:Integrases; D027081:DNA Gyrase