Full length and delta lactoferrin display differential cell localization dynamics, but do not act as tumor markers or significantly affect the expression of other genes.
Lactoferrin is a secreted protein related to transferrin. Lactoferrin indirectly protects host cells against foreign insults by killing bacteria, scavenging free iron, and binding to receptors required for viral invasion. However, lactoferrin is also proposed to act directly on cells as a transcription factor and tumor suppressor gene. In addition to full length lactoferrin, a truncated form, called delta lactoferrin, can also be produced by alternative splicing. We show here that transformed and nontransformed cells are equally able to express both full length and delta lactoferrin. Moreover, both forms of lactoferrin failed to substantially modulate the expression of other genes. Thus, lactoferrin does not seem to directly control gene expression or inhibit tumor cell growth.
Goldberg
Gary S
GS
Department of Molecular Biology, University of Medicine and Dentistry of New Jersey, 2 Medical Center Drive, Stratford, NJ 08084, USA. gary.goldberg@stonybrook.edu
Kunimoto
Takehiko
T
Alexander
David B
DB
Suenaga
Kayoko
K
Ishidate
Fumiyoshi
F
Miyamoto
Kazuaki
K
Ushijima
Toshikazu
T
Teng
Christina T
CT
Yokota
Jun
J
Ohta
Tsutomu
T
Tsuda
Hiroyuki
H
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000737:chemistry; D005347:Fibroblasts / Q000737:chemistry* / Q000378:metabolism; D017353:Gene Deletion; D005786:Gene Expression Regulation; D007781:Lactoferrin / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data
D014408:Biomarkers, Tumor; D007781:Lactoferrin