[Effects of magnetic resonance imaging on neutrophil functions].
We evaluated the effects of magnetic resonance (MR) imaging on human neutrophil functions. In addition of the counting of leukocyte and neutrophil number, expression levels of adhesion molecules on neutrophil surface such as CD11b and L-selectin, and reactive oxygen species (ROS) production were determined by means of flowcytometry. Complete blood count did not show any difference between before and after MR imaging in five normal healthy volunteers. The levels of cell surface adhesion molecules were not altered in both in vivo MR imaging (n=5) and in vitro MR exposure experiments (n=13). Moreover, the levels of ROS production were also not affected by in vivo MR imaging. On the other hand, neutrophils exposed to MR in vitro exhibited significant increase in ROS production after stimulation with fMLP combined with lipopolysaccharide, although no increase was observed with PMA stimulation. Actually, there have been no reports describing the complication relating to hyper-neutrophil function as far as we could search, but it might be necessary to evaluate the biological effects of MR imaging especially under the pathologic circumstances that induce neutrophil activation.
Saigo
Katsuyasu
K
Blood Transfusion Division, Kobe University Hospital, Kobe 650-0017.
Jyokei
Yukie
Y
Hashimoto
Makoto
M
Kishimoto
Manami
M
Matsunaga
Kyoko
K
Takenokuchi
Mariko
M
Sakurai
Kosuke
K
Kawamitsu
Hideaki
H
Fujii
Masahiko
M
Kumagai
Shunichi
S
D000328:Adult; D015815:Cell Adhesion Molecules / Q000032:analysis; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D015815:Cell Adhesion Molecules; D017382:Reactive Oxygen Species