Screening for human papillomavirus (HPV) in Egyptian women by the second-generation hybrid capture (HC II) test.
BACKGROUND
HPV infection is the main cause of cervical cancer and cervical intraepithelial neoplasia worldwide. The second-generation HC II test is a liquid hybridization assay designed to detect 18 HPV types. The aim of the present study was to detect the rate of HPV infection and its various genotypes among Egyptian women.


METHODS
We evaluated 166 Egyptian women. They were classified according to cytology into those with normal cytology, chronic nonspecific cervicitis, and squamous intraepithelial lesions (SILs).


RESULTS
The overall prevalence of HPV DNA in the studied groups was 15.06% (25/166). Among the 25 HPV-positive women, 16 (64%) were infected with high-risk HPV types, 4 (16%) with low risk HPV types, while 5 (20%) had both types. Twenty-one (84%) of the infected women harbored at least one high-risk HPV type, while 9 (36%) harbored at least one low-risk HPV type. Values of HPV viral load for low-risk HPV infection showed no significant difference in the normal and chronic nonspecific cervicitis groups. But when HPV viral load of high-risk HPV infection was compared in the normal, chronic nonspecific cervicitis, and SIL groups, a significant difference was found. The same was detected between chronic nonspecific cervicitis and SIL and between normal cytology and SIL, suggesting an association between viral load and risk of SIL and, accordingly, cancer.


CONCLUSIONS
It may be concluded that HPV testing using the HC II assay is a useful tool when combined with cytology in the diagnosis of high-risk HPV viral types in apparently normal tissues.
Abdel Aziz
Mohamed Talaat
MT
Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt. talaatabdaziz@yahoo.uk.co
Abdel Aziz
Mohamed Zayed
MZ
Atta
Hazem Mahmoud
HM
Shaker
Olaf Gamil
OG
Abdel Fattah
Manal Mohsen
MM
Mohsen
Gamal Ahmed
GA
Ahmed
Hanan Hosni
HH
El Derwi
Douaa Ahmed
DA
D000328:Adult; D004279:DNA, Viral / Q000032:analysis; D004534:Egypt; D005260:Female; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral