title: Compensatory effect of the minor Streptomyces lividans type I signal peptidases on the SipY major signal peptidase deficiency as determined by extracellular proteome analysis.
abstract: The developmentally complex bacterium Streptomyces lividans has the ability to produce and secrete a significant amount of protein and possesses four different type I signal peptidase genes (sipW, sipX, sipY and sipZ) that are unusually clustered in its chromosome. 2-DE and subsequent MS of extracellular proteins showed that proteins with typical export signals for type I and type II signal peptidases are the main components of the S. lividans secretome. Secretion of extracellular proteins is severely reduced in a strain deficient in the major type I signal peptidase (SipY). This deficiency was efficiently compensated by complementation with any of the other three signal peptidases as deduced from a comparison of the corresponding 2-D PAGE patterns with that of the wild-type strain.
authors.affiliation: Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, Cantoblanco, Madrid, Spain.
authors.forename: Marta R
authors.initials: MR
authors.lastname: Escutia
authors.forename: Gema
authors.initials: G
authors.lastname: Val
authors.forename: Arantxa
authors.initials: A
authors.lastname: Palacín
authors.forename: Nick
authors.initials: N
authors.lastname: Geukens
authors.forename: Jozef
authors.initials: J
authors.lastname: Anné
authors.forename: Rafael P
authors.initials: RP
authors.lastname: Mellado
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D012697:Serine Endopeptidases / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D048170:Streptomyces lividans / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D020543:Proteome; D012697:Serine Endopeptidases; C026515:type I signal peptidase