Gene expression of human T lymphocytes cell cycle: experimental and bioinformatic analysis.
Human lymphocytes gene expression is monitored before and after PHA stimulation over 72 h, using DNA microarray technology. Results are then compared with our previous bioinformatics predictions, which identified six leader genes of highest importance in human T lymphocytes cell cycle. Experimental data are strikingly compatible with bioinformatic predictions of the specific role and interaction of PCNA, CDC2, and CCNA2 at all phases of the cell cycle and of CHEK1 in regulating DNA repair and preservation. It does not escape our notice that the conception and use of ad hoc arrays, based on a bioinformatics prediction which identifies the most important genes involved in a particular biological process, can really be an added value in cell biology and cancer research alternative to massive frequently misleading molecular genomics.
Giacomelli
Luca
L
Nanoworld Institute, University of Genoa, Corso Europa 30, 16132 Genoa, Italy.
Nicolini
Claudio
C
D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism; D019295:Computational Biology; D019925:Cyclin A / Q000235:genetics / Q000378:metabolism; D056751:Cyclin A2; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*
C503721:CCNA2 protein, human; D019925:Cyclin A; D056751:Cyclin A2; D018809:Proliferating Cell Nuclear Antigen; D016203:CDC2 Protein Kinase