title: Mice strain differences in effects of fetal exposure to diesel exhaust gas on male gonadal differentiation.
abstract: We have shown that in ICR pregnant mice exposed to diesel exhaust (DE), mRNA expression of mällerian inhibiting substance (MIS) and a steroid hormone transcription factor (Ad4BP/SF-1), which are essential in male gonadal differentiation, decreases in a DE concentration-dependent manner. To further investigate whether these effects differ among strains, we conducted the present study in 3 different strains: ICR mice, ddY mice, and C57BL/6J mice. The response to DE exposure differed among the 3 strains. In C57BL/6J male fetuses, only MIS mRNA expression significantly decreased, and in ddY male fetuses, there was no change in either MIS or Ad4BP/SF-1 mRNA expression. Although there was no definite correlation between mouse strain characteristics and differences in the effects of DE, our findings suggest strain-related variations in DE sensitivity with respect to gene expression regulating male gonadal differentiation.
authors.affiliation: Department of Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-0022, Japan. syoshida@oita-nhs.ac.jp
authors.forename: Seiichi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Miki
authors.initials: M
authors.lastname: Yoshida
authors.forename: Isamu
authors.initials: I
authors.lastname: Sugawara
authors.forename: Ken
authors.initials: K
authors.lastname: Takeda
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008431:Maternal-Fetal Exchange; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D012733:Sex Differentiation; D013045:Species Specificity; D054339:Steroidogenic Factor 1; D013735:Testicular Hormones / Q000235:genetics / Q000378:metabolism; D013737:Testis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D006023:Glycoproteins; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D013735:Testicular Hormones; D014157:Transcription Factors; D001335:Vehicle Emissions; D054304:Anti-Mullerian Hormone