title: GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactis.
abstract: We show that the nitrogen regulatory protein GlnR of Lactococcus lactis represses transcription of the amtB-glnK, glnRA, and glnPQ operons. This likely occurs through a conserved DNA motif, 5'-TGTNA-7N-TNACAT-3', and takes place in response to extracellular glutamine and ammonium. GlnR-independent repression of amtB-glnK is mediated by the pleiotropic nitrogen regulator CodY.
authors.affiliation: Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
authors.forename: Rasmus
authors.initials: R
authors.lastname: Larsen
authors.forename: Tomas G
authors.initials: TG
authors.lastname: Kloosterman
authors.forename: Jan
authors.initials: J
authors.lastname: Kok
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D005973:Glutamine; D013294:Lactococcus lactis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D009876:Operon / Q000235:genetics; D000644:Quaternary Ammonium Compounds; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D000644:Quaternary Ammonium Compounds; D015534:Trans-Activators; D005973:Glutamine; D009584:Nitrogen