title: Sequence stability of the T-DNA - plant junctions in tissue culture in Arabidopsis transgenic lines.
abstract: The stability of the inserted transgenes and particularly the junction regions of transgenic events is a concern of food labeling, traceability and post release monitoring, as these regions are used for development of event-specific DNA-based detection methods. During the standard agricultural breeding practices, the transgenic lines can be exposed to completely different conditions than those in the laboratory environment. Some of these conditions have the potential to affect the stability of the transgenic locus and the surrounding DNA. As tissue culture is recognized as a stressful and mutagenic factor, we have analyzed the effect of this process on the stability of the junction regions at nucleotide level in five Arabidopsis thaliana transgenic lines in comparison with the respective integration loci in ColO and C24 ecotypes. No indication of any kind of alteration at nucleotide level of the junctions was found. The relevance of the stability of the plant-T-DNA junction regions for application of the DNA-based methods in commercial transgenic plants is discussed.
authors.affiliation: Unit Technology and Food, Institute for Agricultural and Fisheries Research, 9820, Merelbeke, Belgium. nina.papazova@ilvo.vlaanderen.be
authors.forename: Nina
authors.initials: N
authors.lastname: Papazova
authors.forename: Pieter
authors.initials: P
authors.lastname: Windels
authors.forename: Ann
authors.initials: A
authors.lastname: Depicker
authors.forename: Isabel
authors.initials: I
authors.lastname: Taverniers
authors.forename: Isabel
authors.initials: I
authors.lastname: Roldan-Ruiz
authors.forename: Anne
authors.initials: A
authors.lastname: Milcamps
authors.forename: Erik
authors.initials: E
authors.lastname: Van Bockstaele
authors.forename: Guy
authors.initials: G
authors.lastname: Van Den Eede
authors.forename: Marc
authors.initials: M
authors.lastname: De Loose
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D030821:Plants, Genetically Modified; D017422:Sequence Analysis, DNA; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA