[Isoelectrofocusing and photocolorimetry for determination of glycated hemoglobin (HbA1c)].
Analytical reliability of a new method for determination of glycated hemoglobin (HbA1c) has been evaluated. The method is based on hemoglobin isolation during isoelectrofocusing followed by acidic hydrolysis and photocolorimetry of furfurol formed after its reaction with thiobarbituric acid. At diabetic and non-diabetic levels inter- and intraserial reproducibility varied from 6 to 21%. Sensitivity of this method was determined using physiological solution and 50% albumin.
Korolev
V A
VA
Molchanov
V I
VI
D003124:Colorimetry / Q000379:methods*; D003920:Diabetes Mellitus / Q000175:diagnosis; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017392:Thiobarbituric Acid Reactive Substances
D006442:Glycated Hemoglobin; D017392:Thiobarbituric Acid Reactive Substances