Evaluation of nephelometry for albumin measurement in serum and cerebrospinal fluid: experiences with an indwelling subarachnoidal catheter system for repetitive cerebrospinal fluid collection in horses.
The measurement of albumin concentrations in cerebrospinal fluid (CSF) and serum for albumin quotient (AQ) calculations in normal horses was performed by 2 methods: 1) total protein measurement, followed by electrophoresis of the samples to obtain an albumin percentage; and 2) albumin immunoprecipitation quantitated by nephelometry. The results of both methods correlated well, and nephelometry was chosen to determine the albumin concentrations in CSF samples obtained from an indwelling subarachnoidal catheter for daily sampling. Because the use of an indwelling catheter to collect repetitive CSF samples is a novel technique, routine cytological CSF analysis was performed along with daily clinical evaluation to ascertain the well-being of the horses. The catheters were placed in 2 horses for periods of 14 and 17 days. One horse exhibited pleocytosis on cytological evaluation of CSF on 2 occasions for a 1-2-day duration; however, the AQ showed a significant increase on only 1 occasion. The other horse had a normal cell count in CSF but showed 2 sudden changes in the AQ value; however, these values remained within the 95% confidence interval for AQ in horses. Albumin quotient values of the second horse were consistently below the lower range of the confidence interval. Results from this study indicate that nephelometry can be used for albumin determination in serum and CSF samples from horses. Furthermore, an indwelling subarachnoidal catheter system can provide serial CSF samples in horses, thus obviating the need for repetitive centesis for serial CSF sampling.
Goehring
L S
LS
Department of Equine Sciences, Discipline Internal Medicine, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. lutz.goehring@colostate.edu
Kessels
B G F
BG
van Maanen
C
C
Voorbij
H A M
HA
Sloet van Oldruitenborgh-Oosterbaan
M M
MM
D000418:Albumins / Q000134:cerebrospinal fluid*; D000818:Animals; D002408:Catheters, Indwelling / Q000662:veterinary; D004587:Electrophoresis, Agar Gel; D005260:Female; D006736:Horses / Q000097:blood* / Q000134:cerebrospinal fluid*; D008137:Longitudinal Studies; D008297:Male; D009391:Nephelometry and Turbidimetry / Q000379:methods / Q000662:veterinary*; D012709:Serum Albumin / Q000032:analysis*; D013346:Subarachnoid Space
D000418:Albumins; D012709:Serum Albumin