title: The use of mass spectrometry for the proteomic analysis of glycosylation.
abstract: Of all protein PTMs, glycosylation is by far the most common, and is a target for proteomic research. Glycosylation plays key roles in controlling various cellular processes and the modifications of the glycan structures in diseases highlight the clinical importance of this PTM. Glycosylation analysis remains a difficult task. MS, in combination with modern separation methodologies, is one of the most powerful and versatile techniques for the structural analysis of glycoconjugates. This review describes methodologies based on MS for detailed characterization of glycoconjugates in complex biological samples at the sensitivity required for proteomic work.
authors.affiliation: Unité Mixte de Recherche CNRS/USTL 8576, Université des Sciences et Technologies de Lille 1, Villeneuve d'Ascq Cedex, France. willy.morelle@univ-lille1.fr
authors.forename: Willy
authors.initials: W
authors.lastname: Morelle
authors.forename: Kévin
authors.initials: K
authors.lastname: Canis
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chirat
authors.forename: Valegh
authors.initials: V
authors.lastname: Faid
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Michalski
mesh_terms: D015415:Biomarkers / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000737:chemistry; D040901:Proteomics / Q000295:instrumentation; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D006020:Glycopeptides; D006023:Glycoproteins; D011134:Polysaccharides