[Cloning of A73 gene and its coding sequence analysis].
OBJECTIVE
To clone A73 gene of Epstein-Barr virus (EBV) and examine the variation of its coding sequence.


METHODS
A73 coding sequence (CDS) amplified from 7 patients in nasophryngeal carcinoma (NPC) biopsies by RT-PCR was cloned into pGEM-T-Easy vector to construct the recombinant plasmid, which was subjected to sequence analysis in Gen Bank database using Blast software.


RESULTS
A73 gene from nasophryngeal carcinoma was successfully cloned into pGEM-T Easy vector. A locus with conversion of A-->C in A73 was found at CDS 45 nt, located in the exonVB157154 nt, which did not result in an amino acid replacement, and the variation frequency was 7/7.


CONCLUSIONS
There is a point mutation in A73 CDS of EBV isolated from NPC tissue, which might produce NPC-associated polymorphism with possible involvement in the composition of some subtype of EB virus.
Li
Ang
A
Department of Thoracic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. angli@hotmail.com
Zhao
Song
S
Hu
Wei
W
Zhao
Gao-feng
GF
Zhang
Xiao-shi
XS
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000473:pathology / Q000821:virology; D016366:Open Reading Frames / Q000235:genetics; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
D018076:DNA, Complementary; D014764:Viral Proteins