Testosterone metabolism in Neomysis integer following exposure to benzo(a)pyrene.
Cytochromes P450 (CYPs) are important enzymes involved in the regulation of hormone synthesis and in the detoxification and/or activation of xenobiotics. CYPs are found in virtually all organisms, from archae, and eubacteria to eukaryota. A number of endocrine disruptors are suspected of exerting their effects through disruption of normal CYP function. Consequently, alterations in steroid hormone metabolism through changes in CYP could provide an important tool to evaluate potential effects of endocrine disruptors. The aim of this study was to investigate the potential effects of the known CYP modulator, benzo(a)pyrene (BaP), on the testosterone metabolism in the invertebrate Neomysis integer (Crustacea; Mysidacea). N. integer were exposed for 96 h to 0.43, 2.39, 28.83, 339.00 and 1,682.86 microg BaP L(-1) and a solvent control, and subsequently their ability to metabolize testosterone was assessed. Identification and quantification of the produced phase I and phase II testosterone metabolites was performed using liquid chromatography coupled with multiple mass spectrometry (LC-MS2). Significant changes were observed in the overall ability of N. integer to metabolize testosterone when exposed to 2.39, 28.83, 339.00 and 1,682.86 microg BaP L(-1) as compared to the control animals.
Poelmans
S
S
Laboratory of Chemical Analysis, Department of Veterinary Public Health and Food Safety, Research Group Veterinary Public Health and Zoonoses, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Verslycke
T
T
Monteyne
E
E
Noppe
H
H
Verheyden
K
K
Janssen
C R
CR
De Brabander
H F
HF
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002853:Chromatography, Liquid; D003445:Crustacea / Q000187:drug effects* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000633:toxicity*; D013058:Mass Spectrometry; D013739:Testosterone / Q000378:metabolism*
D052244:Endocrine Disruptors; D001564:Benzo(a)pyrene; D013739:Testosterone; D003577:Cytochrome P-450 Enzyme System