[Determination of octafluoropropane content in human albumin micropheres loaded with octafluoropropane].
OBJECTIVE
To develop a method for determinating the octafluoropropane (OFP) content in human albumin micropheres loaded with OFP.


METHODS
OFP content in the loaded human albumin micropheres was determined by means of gas chromatography coupled with mass spetrum/selected ion monitoring (GC-MS/SIM).


RESULTS
The calibration curve was linear within the concentration range of 40.92-16,368 nmol/L (R(2)=0.9999). The limit of detection was 8.18 nmol/L, and the recovery rate was (100.98-/+1.4)% (204 nmol/L), (102.63-/+2.29)% (2,046 nmol/L) and (97.5-/+2.2)% (8,184 nmol/L), respectively. The intra-day and inter-day RSDs were 0.6%-2.0% and 0.86%-2.3%, respectively.


CONCLUSIONS
The method of GC-MS/SIM is accurate, sensitive, precise and applicable for OFP determination in the microspheres.
Yang
Ling
L
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Chen
Zhi-liang
ZL
D003287:Contrast Media / Q000032:analysis*; D004452:Echocardiography; D005466:Fluorocarbons / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008863:Microspheres; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000737:chemistry*
D003287:Contrast Media; D005466:Fluorocarbons; D012709:Serum Albumin; C042852:perflutren