Dynamics of dendritic cell migration and the subsequent induction of protective immunity in the lung after repeated airway challenges by nontypeable Haemophilus influenzae outer membrane protein.
To determine the dynamics of dendritic cell (DCs) migration and their role in recurrent infections by nontypeable Haemophilus influenzae (NTHi), the migration of mature DC into pulmonary lymph nodes (LN) and the development of a P6-specific immune response and bacterial clearance in the lung were examined after repeated airway challenges with outer membrane protein (OMP) at 1-week intervals in mice. Although the migration of mature DC into the pulmonary LN is attenuated after repeated airway challenge with OMP, the in vitro P6-specific T cell proliferation in the cultured pulmonary LN cells was enhanced and was subsequently linked to the development of P6-specific IgA production and the development of protective immunity in the airway of mice.
Kurita
Shin-ichi
S
Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
Koyama
Jun
J
Onizuka
Shozaburo
S
Motomura
Kazushi
K
Watanabe
Hiroshi
H
Watanabe
Kiwao
K
Senba
Masachika
M
Apicella
Michael A
MA
Murphy
Timothy F
TF
Yoneyama
Horoyuki
H
Matsushima
Kouji
K
Nagatake
Tsuyoshi
T
Oishi
Kazunori
K
D000280:Administration, Inhalation; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D006192:Haemophilus Infections / Q000276:immunology* / Q000517:prevention & control; D018073:Haemophilus Vaccines / Q000276:immunology*; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000276:immunology*; D051379:Mice
D001425:Bacterial Outer Membrane Proteins; D018073:Haemophilus Vaccines