Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp. isolated from the Bay of Bengal.
Four marine actinobacteria tolerant to 200 g NaCl l(-1) were screened for antibacterial activity against eight patient-derived multiple drug resistant (MDR) bacteria. The active compound (MW 300.2, predicted molecular formula C(20)H(28)O(2)) from an actinobacterium, was inhibitory to three Gram-positive and three Gram-negative MDR bacteria, seven non-clinical Gram-positive, four Gram-negative bacteria and five fungi (MIC: 3.5-4.0 microg ml(-1)). Also, 54% of human leukemia (HL-60) cells were killed by the compound at 0.05 microg ml(-1). Bioreactor production demonstrated unusual primary metabolite kinetics. Molecular phylogenetic analysis showed this typical intertidal inhabitant to be a member of the Streptomyces genus and distinct from other salt-tolerant actinobacteria. As no compound was found to match the properties in several electronic databases, our screening strategy should increase the possibility of discovering bioactive molecules from rare actinobacteria.
Saha
Malay
M
Environmental Science Programme and Department of Life Science & Biotechnology, Jadavpur University, Kolkata, India.
Jaisankar
Parasuraman
P
Das
Satadal
S
Sarkar
Kalyan K
KK
Roy
Soma
S
Besra
Shila E
SE
Vedasiromani
Joseph R
JR
Ghosh
Debashish
D
Sana
Barindra
B
Mukherjee
Joydeep
J
D039903:Actinobacteria / Q000187:drug effects / Q000378:metabolism*; D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004351:Drug Resistance; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D008969:Molecular Sequence Data; D012965:Sodium Chloride / Q000494:pharmacology
D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D012965:Sodium Chloride