title: Accumulation of glycinebetaine in rice plants that overexpress choline monooxygenase from spinach and evaluation of their tolerance to abiotic stress.
abstract: OBJECTIVE
  Glycinebetaine (GB), a quaternary ammonium compound, is a very effective compatible solute. In higher plants, GB is synthesized from choline (Cho) via betaine aldehyde (BA). The first and second steps in the biosynthesis of GB are catalysed by choline monooxygenase (CMO) and by betaine aldehyde dehydrogenase (BADH), respectively. Rice (Oryza sativa), which has two genes for BADH, does not accumulate GB because it lacks a functional gene for CMO. Rice plants accumulate GB in the presence of exogenously applied BA, which leads to the development of a significant tolerance to salt, cold and heat stress. The goal in this study was to evaluate and to discuss the effects of endogenously accumulated GB in rice.
  
  
  METHODS
  Transgenic rice plants that overexpressed a gene for CMO from spinach (Spinacia oleracea) were produced by Agrobacterium-mediated transformation. After Southern and western blotting analysis, GB in rice leaves was quantified by (1)H-NMR spectroscopy and the tolerance of GB-accumulating plants to abiotic stress was investigated.
  
  
  RESULTS
  Transgenic plants that had a single copy of the transgene and expressed spinach CMO accumulated GB at the level of 0.29-0.43 micromol g(-1) d. wt and had enhanced tolerance to salt stress and temperature stress in the seedling stage.
  
  
  CONCLUSIONS
  In the CMO-expressing rice plants, the localization of spinach CMO and of endogenous BADHs might be different and/or the catalytic activity of spinach CMO in rice plants might be lower than it is in spinach. These possibilities might explain the low levels of GB in the transgenic rice plants. It was concluded that CMO-expressing rice plants were not effective for accumulation of GB and improvement of productivity.
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, Aoba, Sendai 981-8555, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Shirasawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takabe
authors.forename: Tetsuko
authors.initials: T
authors.lastname: Takabe
authors.forename: Sachie
authors.initials: S
authors.lastname: Kishitani
mesh_terms: D001622:Betaine / Q000737:chemistry / Q000378:metabolism*; D015139:Blotting, Southern; D015153:Blotting, Western; D017343:Genes, Plant; D009682:Magnetic Resonance Spectroscopy; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010105:Oxygenases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified; D012231:Rhizobium / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism*; D018724:Spinacia oleracea / Q000201:enzymology / Q000235:genetics; D013696:Temperature; D014170:Transformation, Genetic
chemical_list: D001622:Betaine; D012965:Sodium Chloride; C105448:choline monooxygenase; D010105:Oxygenases