Inhibition of sodium glucose cotransporter-I expressed in Xenopus laevis oocytes by 4-acetoxyscirpendiol from Cordyceps takaomantana (anamorph = Paecilomyces tenuipes).
Cordyceps contains many health-promoting constituents. Recent studies revealed that the fruiting body of cordyceps significantly alleviates hyperglycemia which usually accompanies diabetes mellitus. The mechanism of the anti-hyperglycemic effect by cordyceps, however, is not fully understood. In this study, methanolic extracts were prepared from fruiting bodies of Paecilomyces tenuipes, and 4-beta acetoxyscirpendiol (ASD) was eventually purified from the extracts. The Na+/ glucose transporter-1 (SGLT-1) was expressed in Xenopus oocytes, and the effect of ASD on it was analyzed using voltage clamp and 2-deoxy-D-glucose (2-DOG) uptake studies. Fluorescence microscopy was performed to monitor the effect of ASD on glucose uptake using HEK293 cells expressing recombinant SGLT-1. ASD inhibited SGLT-1 activity, and its two derivatives (2-acetoxyscirpenol and 15-acetoxyscirpendiol), were also effective; 15-acetoxyscirepenol was as inhibitory as ASD while diacetoxyscirpenol had less effect. Thus, the ASD in P. tenuipes may play an important role in lowering blood sugar in the circulatory system along with its derivatives as specific inhibitors of SGLT-1.
Yoo
Ocki
O
Department of Life Sciences, University of Seoul, 90 Jeonnong-Dong, Dongdaemun-Gu, Seoul 130-743, Korea.
Lee
Dong-Hee
DH
D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology; D002460:Cell Line; D032661:Cordyceps / Q000737:chemistry*; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D009183:Mycotoxins / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects; D018408:Patch-Clamp Techniques; D051269:Sodium-Glucose Transporter 1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014255:Trichothecenes / Q000494:pharmacology; D014982:Xenopus laevis
C482282:4beta-acetoxyscirpendiol; D001578:Benzopyrans; D009183:Mycotoxins; D051269:Sodium-Glucose Transporter 1; D014255:Trichothecenes; C510382:acetoxyscirpenol; D003847:Deoxyglucose; D013605:T-2 Toxin; D005947:Glucose; C001809:diacetoxyscirpenol