title: The influence of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) on potato tuber metabolism.
abstract: The aim of this work was to investigate the importance of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) in potato carbohydrate metabolism. For this purpose, the cytosolic isoform of phosphorylating GAPC was cloned and used for an antisense approach to generate transgenic potato plants that exhibited constitutively decreased GAPDH activity. Potato lines with decreased activities of phosphorylating GAPC exhibited no major changes in either whole-plant or tuber morphology. However, the levels of 3-phosphoglycerate were decreased in leaves of the transformants. A broad metabolic phenotyping of tubers from the transformants revealed an increase in sucrose and UDPglucose content, a decrease in the glycolytic intermediates 3-phosphoglycerate and phosphoenolpyruvate but little change in the levels of other metabolites. Moreover, the transformants displayed no differences in cold sweetening with respect to the wild type. Taken together these data suggest that phosphorylating GAPC plays only a minor role in the regulation of potato metabolism. The results presented here are discussed in relation to current models regarding primary metabolism in the potato tuber parenchyma.
authors.affiliation: Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany. mohammad@ipk-gatersleben.de
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Hajirezaei
authors.forename: Sophia
authors.initials: S
authors.lastname: Biemelt
authors.forename: Martin
authors.initials: M
authors.lastname: Peisker
authors.forename: Anna
authors.initials: A
authors.lastname: Lytovchenko
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.forename: Uwe
authors.initials: U
authors.lastname: Sonnewald
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism / Q000502:physiology*; D002250:Carbon Radioisotopes / Q000378:metabolism; D019069:Cell Respiration / Q000502:physiology; D003080:Cold Temperature; D003600:Cytosol / Q000201:enzymology; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000378:metabolism*; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism; D035281:Plant Tubers / Q000201:enzymology / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)