title: The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly.
abstract: The Nup107-160 complex is a critical subunit of the nuclear pore. This complex localizes to kinetochores in mitotic mammalian cells, where its function is unknown. To examine Nup107-160 complex recruitment to kinetochores, we stained human cells with antisera to four complex components. Each antibody stained not only kinetochores but also prometaphase spindle poles and proximal spindle fibers, mirroring the dual prometaphase localization of the spindle checkpoint proteins Mad1, Mad2, Bub3, and Cdc20. Indeed, expanded crescents of the Nup107-160 complex encircled unattached kinetochores, similar to the hyperaccumulation observed of dynamic outer kinetochore checkpoint proteins and motors at unattached kinetochores. In mitotic Xenopus egg extracts, the Nup107-160 complex localized throughout reconstituted spindles. When the Nup107-160 complex was depleted from extracts, the spindle checkpoint remained intact, but spindle assembly was rendered strikingly defective. Microtubule nucleation around sperm centrosomes seemed normal, but the microtubules quickly disassembled, leaving largely unattached sperm chromatin. Notably, Ran-GTP caused normal assembly of microtubule asters in depleted extracts, indicating that this defect was upstream of Ran or independent of it. We conclude that the Nup107-160 complex is dynamic in mitosis and that it promotes spindle assembly in a manner that is distinct from its functions at interphase nuclear pores.
authors.affiliation: Sections of Cell and Developmental Biology, Division of Biological Sciences, University of California-San Diego Medical School, La Jolla, CA 92093-0347, USA.
authors.forename: Arturo V
authors.initials: AV
authors.lastname: Orjalo
authors.forename: Alexei
authors.initials: A
authors.lastname: Arnaoutov
authors.forename: Zhouxin
authors.initials: Z
authors.lastname: Shen
authors.forename: Yekaterina
authors.initials: Y
authors.lastname: Boyarchuk
authors.forename: Samantha G
authors.initials: SG
authors.lastname: Zeitlin
authors.forename: Beatriz
authors.initials: B
authors.lastname: Fontoura
authors.forename: Steven
authors.initials: S
authors.lastname: Briggs
authors.forename: Mary
authors.initials: M
authors.lastname: Dasso
authors.forename: Douglass J
authors.initials: DJ
authors.lastname: Forbes
mesh_terms: D000818:Animals; D002457:Cell Extracts; D016764:Cell Polarity; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D049468:Prometaphase; D021381:Protein Transport; D008941:Spindle Apparatus / Q000378:metabolism*; D014981:Xenopus / Q000378:metabolism; D029867:Xenopus Proteins / Q000378:metabolism*
chemical_list: D002457:Cell Extracts; C512772:NUP107 protein, human; C440251:NUP160 protein, human; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; C499080:Nup160 protein, Xenopus; D029867:Xenopus Proteins