title: Detection of the marine toxin okadaic acid in mussels during a diarrhetic shellfish poisoning (DSP) episode in Thermaikos Gulf, Greece, using biological, chemical and immunological methods.
abstract: An approach involving chemical and biological techniques was taken for the detection and quantification of the marine toxin okadaic acid (OA) in mussels from Thermaikos Gulf, Greece, during a 4-month DSP episode that occurred in 2002. Samples were analyzed using the mouse bioassay, high performance liquid chromatography (HPLC) with fluorimetric detection and an enzyme-linked immunosorbent assay (ELISA). Okadaic acid was quantifiable at three different sites of Thermaikos Gulf reaching a maximum concentration of 36 microg/g hepatopancreas. High correlation was revealed between results derived from HPLC and ELISA (R2=0.998), while 91% consistency between HPLC and the mouse bioassay results was observed.
authors.affiliation: National Reference Laboratory on Marine Biotoxins, Center of Veterinary Institutions of Thessaloniki, Ministry of Agriculture, Limnou 3A, 546 27 Thessaloniki, Greece. thmourat@chem.auth.gr
authors.forename: Theoni
authors.initials: T
authors.lastname: Mouratidou
authors.forename: Ignatia
authors.initials: I
authors.lastname: Kaniou-Grigoriadou
authors.forename: Constantini
authors.initials: C
authors.lastname: Samara
authors.forename: Themistokles
authors.initials: T
authors.lastname: Kouimtzis
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003967:Diarrhea / Q000209:etiology; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination; D006115:Greece; D008387:Marine Toxins / Q000032:analysis* / Q000633:toxicity*; D051379:Mice; D049878:Mytilus; D019319:Okadaic Acid / Q000032:analysis* / Q000633:toxicity* / Q000031:analogs & derivatives; D011714:Pyrans / Q000032:analysis; D057096:Shellfish Poisoning
chemical_list: D008387:Marine Toxins; D011714:Pyrans; D019319:Okadaic Acid; C051904:dinophysistoxin 1