title: Differential sensitivity of in vivo and ex vivo diffusion tensor imaging to evolving optic nerve injury in mice with retinal ischemia.
abstract: Decreased axial (lambda(||)) and increased radial (lambda( perpendicular)) diffusivity have been shown to reflect axonal and myelin injury respectively. In the present study, evolving white matter injury within the optic nerves of mice with retinal ischemia was examined by in vivo and ex vivo measurements of lambda(||) and lambda( perpendicular). The results show that at 3 days after retinal ischemia, a 33% decrease in vivo and a 38% decrease ex vivo in lambda(||) without change in lambda( perpendicular) was observed in the injured optic nerve compared to the control, suggestive of axonal damage without myelin injury. At 14 days, both in vivo and ex vivo measured lambda( perpendicular) increased significantly to 220-240% of the control level in the injured optic nerve suggestive of myelin damage. In contrast, the axonal injury that was clearly detected in vivo as a significantly decreased lambda(||) (33% decrease) was not as clearly detected by ex vivo lambda(||) (17% decrease). The current findings suggest that ex vivo lambda( perpendicular) is comparable to in vivo lambda( perpendicular) in detecting myelin injury. However, the structural changes resulting from axonal damage causing the decreased in vivo lambda(||) may not be preserved ex vivo in the fixed tissues. Despite the accurate depiction of the pathology using lambda(||) and lambda( perpendicular) in vivo, the use of ex vivo lambda(||) to extrapolate the status of axonal injury in vivo would require further investigation.
authors.affiliation: Department of Radiology, Biomedical MR Laboratory, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA. richard@bmr.wustl.edu
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Sun
authors.forename: Hsiao-Fang
authors.initials: HF
authors.lastname: Liang
authors.forename: Tuan Q
authors.initials: TQ
authors.lastname: Le
authors.forename: Regina C
authors.initials: RC
authors.lastname: Armstrong
authors.forename: Anne H
authors.initials: AH
authors.lastname: Cross
authors.forename: Sheng-Kwei
authors.initials: SK
authors.lastname: Song
mesh_terms: D000465:Algorithms; D000818:Animals; D001369:Axons / Q000473:pathology; D001921:Brain / Q000473:pathology; D003627:Data Interpretation, Statistical; D038524:Diffusion Magnetic Resonance Imaging; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009186:Myelin Sheath / Q000473:pathology; D009410:Nerve Degeneration / Q000473:pathology; D016900:Neurofilament Proteins / Q000378:metabolism; D009901:Optic Nerve Diseases / Q000209:etiology / Q000473:pathology*; D018917:Optic Neuropathy, Ischemic / Q000150:complications / Q000473:pathology*; D016707:Tissue Fixation
chemical_list: D016900:Neurofilament Proteins