Determination of minor proteins of bovine milk and colostrum by optical biosensor analysis.
Automated, rapid, sensitive, and label-free biosensor-based immunoassays for immunoglobulin G (IgG), folate binding protein, lactoferrin, and lactoperoxidase in bovine milk using surface plasmon resonance optical detection with direct binding assay format are described. Samples are prepared for analysis by direct dilution into buffer. Analysis conditions, including ligand immobilization, flow rate, contact time, and regeneration are defined and nonspecific binding considerations evaluated. The technique has been applied to the measurement of these proteins in consumer milks, colostrum, milk products, and infant formulas, and their temporal change during early bovine lactation followed.
Indyk
Harvey E
HE
Fonterra, PO Box 7, Waitoa, New Zealand. Harvey.Indyk@fonterra.com
Filonzi
Enrico L
EL
Gapper
Leyton W
LW
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002138:Calibration; D002352:Carrier Proteins / Q000032:analysis; D002417:Cattle; D003126:Colostrum / Q000378:metabolism*; D058973:Folate Receptors, GPI-Anchored; D007074:Immunoglobulin G / Q000032:analysis; D007781:Lactoferrin / Q000032:analysis; D007784:Lactoperoxidase / Q000032:analysis; D008024:Ligands; D008894:Milk Proteins / Q000032:analysis* / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D007074:Immunoglobulin G; D008024:Ligands; D008894:Milk Proteins; D011956:Receptors, Cell Surface; D007784:Lactoperoxidase; D007781:Lactoferrin