title: Salicylic acid enhances Jaceosidin and Syringin production in cell cultures of Saussurea medusa.
abstract: Addition of 20 muM salicylic acid to Saussurea medusa cell cultures at day 6 resulted in jaceosidin and syringin productions up to 95 mg l(-1 )and 631 mg l(-1) which were, respectively, about 2.5- and 2.7-fold higher than in the control. The biomass was increased from 8 to 12 g l(-1). Expression of chalcone synthase gene (chs) increased sharply after 12 h treatment and was sustained up to 48 h; chalcone isomerase gene (chi) expression reached a peak at 24 h and decreased after 48 h; and phenylalanine ammonia-lyase activity increased by 7.5-fold (96 U mg(-1) protein) higher than in the control after 24 h. These results indicate that salicylic acid enhances the production of jaceosidin and syringin which is accompanied by induction of the related phenylpropanoid biosynthetic enzymes.
authors.affiliation: Key Laboratory for Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing 100093, China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Yu
authors.forename: Chun-Xiang
authors.initials: CX
authors.lastname: Fu
authors.forename: Ying-Shan
authors.initials: YS
authors.lastname: Han
authors.forename: Yong-Xing
authors.initials: YX
authors.lastname: Li
authors.forename: De-Xiu
authors.initials: DX
authors.lastname: Zhao
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000302:isolation & purification / Q000378:metabolism*; D005960:Glucosides / Q000302:isolation & purification / Q000378:metabolism*; D010666:Phenylpropionates / Q000302:isolation & purification / Q000378:metabolism*; D020156:Salicylic Acid / Q000008:administration & dosage*; D030441:Saussurea / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D005960:Glucosides; D010666:Phenylpropionates; C477508:jaceosidin; C028305:syringin; D020156:Salicylic Acid