title: Biodegradation of phenol and sodium salicylate mixtures by suspended Pseudomonas putida CCRC 14365.
abstract: The biodegradation of single phenol and sodium salicylate (SA) and their binary mixtures in water by free Pseudomonas putida (P. putida) CCRC 14365 was experimentally studied at 30 degrees C and pH 7. The initial concentration of the cells, adapted with either phenol or SA, was maintained at 0.025 g/L. Single substrate experiments were performed in the substrate level range 0.53-3.18 mM. The Haldane model has shown that phenol was biodegraded more quickly (mu(max)=0.245 h(-1)) than SA (0.137 h(-1)) under the ranges studied, and SA had a more inhibitory effect on cell growth (K(I)=5.21 mM) than phenol (12.6 mM) at low substrate levels even by SA-adapted cells. Binary substrate experiments were carried out at two fixed total substrate levels of 1.06 and 3.18 mM, with a varying molar concentration ratio of 0.33-3.0. The presence of a small amount of phenol to SA could significantly enhance the biodegradation of SA, particularly when the phenol-adapted cells were employed. On the other hand, the addition of a small amount of SA to phenol would retard the biodegradation of phenol, especially at higher total substrate levels (3.18 mM).
authors.affiliation: Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li 320, Taiwan.
authors.forename: Shang-Yuan
authors.initials: SY
authors.lastname: Tsai
authors.forename: Ruey-Shin
authors.initials: RS
authors.lastname: Juang
mesh_terms: D001673:Biodegradation, Environmental; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D007700:Kinetics; D010636:Phenols / Q000378:metabolism*; D016958:Pseudomonas putida / Q000378:metabolism*; D012980:Sodium Salicylate / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D007220:Industrial Waste; D010636:Phenols; D014874:Water Pollutants, Chemical; D012980:Sodium Salicylate