title: Analysis of non-B DNA structure at chromosomal sites in the mammalian genome.
abstract: Changes at sites of genetic instability ultimately involve DNA repair pathways. Some sites of genetic instability in the mammalian genome appear to be unstable because they adopt a non-B DNA conformation. We describe two structural approaches for determination of whether a genomic region is configured in a non-B DNA conformation. Our studies indicate that at least some chromosomal fragile sites can be explained by such altered DNA conformations. One of the methods that we describe is called the bisulfite modification assay. This is a powerful assay because it provides information on individual DNA molecules. The second approach uses preexisting DNA structural reagents, but describes our specific application of them to analysis of DNA in vivo.
authors.affiliation: Department of Pathology, University of Southern California, Keck School of Medicine, Los Angeles, USA.
authors.forename: Sathees C
authors.initials: SC
authors.lastname: Raghavan
authors.forename: Albert
authors.initials: A
authors.lastname: Tsai
authors.forename: Chih-Lin
authors.initials: CL
authors.lastname: Hsieh
authors.forename: Michael R
authors.initials: MR
authors.lastname: Lieber
mesh_terms: D000818:Animals; D001483:Base Sequence; D043283:Chromosome Fragile Sites; D004247:DNA / Q000737:chemistry*; D016678:Genome; D007202:Indicators and Reagents / Q000737:chemistry; D008322:Mammals / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction
chemical_list: D007202:Indicators and Reagents; D004247:DNA