Nitric oxide synthase inhibitor attenuates number of regenerating spinal motoneurons in adult rats.
Neuronal survival and death-related effects of nitric oxide synthase are widely studied, yet its potential involvement in regeneration remains largely unexplored. In the present study, the regenerative role of nitric oxide synthase in injured motoneurons was investigated. A ventral root was avulsed and a piece of peripheral nerve was implanted into the spinal cord. Results showed that nitric oxide synthase inhibitor reduced the number of regenerating motoneurons to half compared with sham-operated control at 2 weeks and 4 weeks after injury, but the rate of axonal regeneration was not affected. Our study adds a new line of evidence that expression of nitric oxide synthase is beneficial to the axonal regeneration of the injured spinal motoneurons.
Chu
Tak-Ho
TH
Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
Wu
Wu-Tian
WT
D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count / Q000379:methods; D003911:Dextrans; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009046:Motor Neurons / Q000187:drug effects*; D009416:Nerve Regeneration / Q000187:drug effects*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000502:physiology*; D010525:Peripheral Nerves / Q000637:transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D012235:Rhodamines; D013119:Spinal Cord Injuries / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery; D013266:Stilbamidines; D013997:Time Factors
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D003911:Dextrans; D004791:Enzyme Inhibitors; C067661:Fluoro-Ruby; D020800:Receptor, Nerve Growth Factor; D012235:Rhodamines; D013266:Stilbamidines; D019001:Nitric Oxide Synthase