title: Association of HLA-DR3 and HLA-DR4 with sinonasal polyposis in Mexican Mestizos.
abstract: OBJECTIVE
  The pathogenesis of sinonasal polyposis (SNP) is not clear; it has been suggested that it is polygenic and multifactorial. The major histocompatibility complex is a useful tool to predict genetic susceptibility to diseases, especially to autoimmune diseases. Since such susceptibility is influenced by ethnicity, it is necessary to have a wide knowledge of the structure of the population to which the patient belongs. The purpose of the study was to determine the association of HLA-DRB1 alleles with sinonasal polyposis in the Mexican Mestizo population.
  
  
  METHODS
  We studied the HLA-DR alleles in 34 adult Mexican Mestizo patients with SNP and compared them to those present in 99 healthy controls.
  
  
  METHODS
  Genomic DNA from mononuclear cells was obtained by using the "salting out" technique and high-resolution DNA typing of the HLA-DRB1 alleles was performed after PCR amplification.
  
  
  RESULTS
  We found a statistically significant increased frequency of the HLA-DRB1*03 allele (P = 0.03, odds ratio [OR] = 2.9, 95% confidence interval [CI]: 1.0-7.8) and of the HLA-DRB1*04 allele (P = 0.009, OR = 2.2, 95% CI: 1.2-4.2) in patients with SNP as compared to controls, and a statistically significant decreased frequency of the HLA-DRB1*08 allele (P = 0.01, OR = 0.2, 95% CI: 0.05-0.8).
  
  
  CONCLUSIONS
  The HLA-DR locus seems to be associated with the genetic susceptibility to develop SNP in Mexicans.
  
  
  METHODS
  B-2b.
authors.affiliation: Department of Otolaryngology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Privada de San Francisco No. 47-1 Barrio San Francisco, San Jerónimo Magdalena Contreras, CP 10810, Mexico City DF, Mexico. jmdelafuente@hotmail.com
authors.forename: Jaqueline
authors.initials: J
authors.lastname: Ramírez-Anguiano
authors.forename: Jesús K
authors.initials: JK
authors.lastname: Yamamoto-Furusho
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Barquera
authors.forename: Olga
authors.initials: O
authors.lastname: Beltrán
authors.forename: Julio
authors.initials: J
authors.lastname: Granados
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D004247:DNA / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D015803:HLA-DR3 Antigen / Q000235:genetics*; D015804:HLA-DR4 Antigen / Q000235:genetics*; D006801:Humans; D015994:Incidence; D008297:Male; D008800:Mexico / Q000208:ethnology; D008875:Middle Aged; D009298:Nasal Polyps / Q000208:ethnology / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
chemical_list: D015803:HLA-DR3 Antigen; D015804:HLA-DR4 Antigen; D004247:DNA