Preparation of a novel composite particles and its application in the fluorescent detection of proteins.
A new fluorescence method for the detection of proteins with novel composite nanoparticles (CdS/PPA) has been developed. The composite nanoparticles have been prepared through an in-situ polymerization method under ultrasonic irradiation. The surface of the composite nanoparticles was covered with functional groups (-COOH). These groups may play a major role in the improving the water solubility and biocompatibility of the nanoparticles. The composite particles is combined with proteins in NaAc-HCl buffer solution (pH=1.99), which can result in strong fluorescence, and the response is linearly proportional to the concentration of proteins. In lambdaem/lambdaex=650 nm/365 nm place (the stoke' shift is 285 nm), its fluorescent strength reaches the maximum. Under the optimum conditions, the linear range is 0.10-20.0 microg.ml(-1) with the detection limit of 41 ng.ml(-1) for HSA, and 0.10-15.0 microg.ml(-1) with the detection limit of 35 ng.ml(-1) for Human gamma-IgG . The method has been applied to the determination of the total protein in human serum samples collected from the hospital and the results are satisfactory.
Chen
Hong-Qi
HQ
College of Chemistry and Material Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, Wuhu, 241000, People's Republic of China.
Wang
Lun
L
Liu
Yan
Y
Wu
Wei-Li
WL
Liang
A-Ni
AN
Zhang
Xiao-Lei
XL
D002138:Calibration; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D011506:Proteins / Q000032:analysis*; D044967:Serum / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D011506:Proteins