title: Oxidative stress parameters in different systemic rheumatic diseases.
abstract: The involvement of oxidative stress in the pathogenesis of rheumatic disorders, such as systemic sclerosis (SSc) and chronic polyarthritides, has been suggested yet not thoroughly verified experimentally. We analysed 4 plasmatic parameters of oxidative stress in patients with SSc (n = 17), psoriatic arthritis (PsA) (n = 10) and rheumatoid arthritis (RA) (n = 9) compared with healthy subjects (n = 22). The biomarkers were: total antioxidant capacity (TAC) measured by ferric reducing antioxidant power (FRAP) method, hydroperoxides determined by ferrous ion oxidation in presence of xylenol orange (FOX) method and sulfhydryl and carbonyl groups assessed by spectrophotometric assays. The results showed significantly increased hydroperoxides in SSc, PsA and RA (3.97 +/- 2.25, 4.87 +/- 2.18 and 5.13 +/- 2.36 micromol L(-1), respectively) compared with the control group (2.31 +/- 1.40 micromol L(-1); P < 0.05). Sulfhydryls were significantly lower in SSc (0.466 +/- 0.081 mmol L(-1)), PsA (0.477 +/- 0.059 mmol L(-1)) and RA (0.439 +/- 0.065 mmol L(-1)) compared with the control group (0.547 +/- 0.066 mmol L(-1); P < 0.05). TAC in all three diseases showed no difference in comparison with controls. Carbonyls were significantly higher in RA than in the control group (32.1 +/- 42 vs 2.21 +/- 1.0 nmol (mg protein)(-1); P < 0.05). The obtained data indicate augmented free radical-mediated injury in these rheumatic diseases and suggest a role for the use of antioxidants in prevention and treatment of these pathologies.
authors.affiliation: Dipartimento di Fisiologia Umana e Farmacologia "Vittorio Erspamer", Università di Roma "La Sapienza", Italy.
authors.forename: Omidreza
authors.initials: O
authors.lastname: Firuzi
authors.forename: Leos
authors.initials: L
authors.lastname: Fuksa
authors.forename: Chiara
authors.initials: C
authors.lastname: Spadaro
authors.forename: Iva
authors.initials: I
authors.lastname: Bousová
authors.forename: Valeria
authors.initials: V
authors.lastname: Riccieri
authors.forename: Antonio
authors.initials: A
authors.lastname: Spadaro
authors.forename: Rita
authors.initials: R
authors.lastname: Petrucci
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Marrosu
authors.forename: Luciano
authors.initials: L
authors.lastname: Saso
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D005260:Female; D005609:Free Radicals / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D050050:Protein Carbonylation; D026361:Reactive Nitrogen Species / Q000097:blood; D017382:Reactive Oxygen Species / Q000097:blood; D012216:Rheumatic Diseases / Q000097:blood* / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000097:blood
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds