title: [Development of methods for detection of H5N1 from human clinical specimens].
abstract: BACKGROUND
  To establish a method for H5N1 RNA detection and laboratory diagnosis of suspected human avian influenza (H5N1) virus infected cases.
  
  
  METHODS
  The typing and sub-typing primers were designed according to M and H5 and N1 gene respectively, and the RT-PCR and real-time PCR were developed using these primers.
  
  
  RESULTS
  The RT-PCR and real-time PCR could be used for H5N1 detection specifically, and there was no cross reaction with other influenza subtypes such as H1 and H3. The sensitivity for RT-PCR and real-time PCR was 1 TCID50 and 0.01 TCID50 respectively. Thirteen laboratory confirmed human H5N1 cases were detected from 42 suspected cases by using these methods.
  
  
  CONCLUSIONS
  The established RT-PCR and real-time PCR methods can be used for laboratory detection of suspected human H5N1 cases.
authors.affiliation: Chinese Influenza Center, Institute for Viral Disease Control and Prevention, State Key Laboratory for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Le-ying
authors.initials: LY
authors.lastname: Wen
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Yu
authors.initials: Y
authors.lastname: Lan
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Zhang
authors.forename: Da-yan
authors.initials: DY
authors.lastname: Wang
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Yao
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.forename: Jia-huai
authors.initials: JH
authors.lastname: Zhang
authors.forename: Yuan-ji
authors.initials: YJ
authors.lastname: Guo
authors.forename: Yue-long
authors.initials: YL
authors.lastname: Shu
mesh_terms: D000818:Animals; D002642:Chick Embryo; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D014764:Viral Proteins