The proteome of dissimilatory metal-reducing microorganism Geobacter sulfurreducens under various growth conditions.
The proteome of Geobacter sulfurreducens, a model for the Geobacter species that predominate in many Fe(III)-reducing subsurface environments, was characterized with ultra high-pressure liquid chromatography and mass spectrometry using accurate mass and time (AMT) tags as well as with more traditional two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Cells were grown under six different growth conditions in order to enhance the potential that a wide range of genes would be expressed. The AMT tag approach was able to identify a much greater number of proteins than could be detected with the 2-D PAGE approach. With the AMT approach over 3,000 gene products were identified, representing about 90% of the total predicted gene products in the genome. A high proportion of predicted proteins in most protein role categories were detected; the highest number of proteins was identified in the hypothetical protein role category. Furthermore, 91 c-type cytochromes of 111 predicted genes in the G. sulfurreducens genome were identified. Differences in the abundance of cytochromes and other proteins under different growth conditions provided information for future functional analysis of these proteins. These results demonstrate that a high percentage of the predicted proteins in the G. sulfurreducens genome are produced and that the AMT tag approach provides a rapid method for comparing differential expression of proteins under different growth conditions in this organism.
Ding
Yan-Huai R
YH
Department of Microbiology, University of Massachusetts, Amherst, MA 01002, USA.
Hixson
Kim K
KK
Giometti
Carol S
CS
Stanley
Ann
A
Esteve-Núñez
Abraham
A
Khare
Tripti
T
Tollaksen
Sandra L
SL
Zhu
Wenhong
W
Adkins
Joshua N
JN
Lipton
Mary S
MS
Smith
Richard D
RD
Mester
Tünde
T
Lovley
Derek R
DR
D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D001431:Bacteriological Techniques; D002851:Chromatography, High Pressure Liquid; D003574:Cytochrome c Group / Q000032:analysis / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005290:Ferric Compounds / Q000378:metabolism; D005650:Fumarates / Q000378:metabolism; D045302:Geobacter / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D005290:Ferric Compounds; D005650:Fumarates; D010446:Peptide Fragments; D020543:Proteome; C025314:ferric citrate; C032005:fumaric acid