Psi, a Temperate Phage of Agrobacterium tumefaciens, Is Mutagenic.
A new temperate bacteriophage (phage Psi) of Agrobacterium tumefaciens strain B91 is described. Many octopine-utilizing strains of A. tumefaciens harbor prophage Psi or a phage that is similar if not identical to it. This phage has a very narrow host range, and we found that its growth is strongly reduced in strains which carry an octopine pTi plasmid. When sensitive bacteria are infected with Psi, 1 to 3% of the survivors carry mutations on the chromosome, as well as on the pTi plasmid. This phenomenon appears to be a direct consequence of lysogenization. The possible mechanisms whereby such Psi-induced mutations occur are discussed.
Expert
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Laboratoire de Biologie Cellulaire, Centre National de Recherches Agronomiques, 78000 Versailles, France.
Tourneur
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