title: Gene expression profiles during heat acclimation in Arabidopsis thaliana suspension-culture cells.
abstract: Thermotolerance is induced by moderated heat acclimation. Suspension cultures of heat-acclimated Arabidopsis thaliana L. (Heynh.), ecotype Columbia, show thermotolerance against lethal heat shock (9 min, 50 degrees C), as evidenced by a chlorophyll assay and fluorescein diacetate staining. To monitor the genome-wide transcriptome changes induced by heat acclimation at 37 degrees C, we constructed an A. thaliana cDNA microarray containing 7,989 unique genes, and applied it to A. thaliana suspension-culture cells harvested at various times (0.5, 1, 2.5, 6, and 16 h) during heat acclimation. Data analysis revealed 165 differentially expressed genes that were grouped into ten clusters. We compared these genes with published and publicly available microarray heat-stress-related data sets in AtGenExpress. Heat-shock proteins were strongly expressed, as previously reported, and we found several of the up-regulated genes encoded detoxification and regulatory proteins. Moreover, the transcriptional induction of DREB2 (dehydration responsive element-binding factor 2) subfamily genes and COR47/rd17 under heat stress suggested cross-talk between the signaling pathways for heat and dehydration responses.
authors.affiliation: Division of Applied Life Science (BK21), Environmental Biotechnology National Core Research Center and PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea.
authors.forename: Chan Ju
authors.initials: CJ
authors.lastname: Lim
authors.forename: Kyung Ae
authors.initials: KA
authors.lastname: Yang
authors.forename: Joon Ki
authors.initials: JK
authors.lastname: Hong
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Choi
authors.forename: Dea-Jin
authors.initials: DJ
authors.lastname: Yun
authors.forename: Jong Chan
authors.initials: JC
authors.lastname: Hong
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Chung
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Moo Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Chae Oh
authors.initials: CO
authors.lastname: Lim
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D002478:Cells, Cultured; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D006358:Hot Temperature
chemical_list: D029681:Arabidopsis Proteins