title: A highly efficient chemoenzymatic approach toward glycoprotein synthesis.
abstract: [reaction: see text] A highly efficient endoglycosidase-catalyzed synthesis of homogeneous glycoproteins is described. By using ribonuclease B as a model system, it was demonstrated that Endo-A could efficiently attach a preassembled oligosaccharide to a GlcNAc-containing protein in a regio- and stereospecific manner, when the corresponding sugar oxazoline was used as the donor substrate. The method allows the synthesis of both natural and tailor-made N-linked glycoproteins in excellent yield.
authors.affiliation: Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland, Baltimore, 21201, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Haijing
authors.initials: H
authors.lastname: Song
authors.forename: Steven
authors.initials: S
authors.lastname: Hauser
authors.forename: Lai-Xi
authors.initials: LX
authors.lastname: Wang
mesh_terms: D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008954:Models, Biological; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D006026:Glycoside Hydrolases