Soluble HLA-G molecules induce apoptosis in natural killer cells.
Membrane-bound human leukocyte antigen-G (HLA-G) molecules are primarily expressed by cytotrophoblasts of the fetus. They are thought to protect the fetus from immunologic attack by the maternal immune system and have recently been associated with transplantation graft acceptance. In addition, soluble HLA-G molecules (sHLA-G) have been shown to play a role in the success of pregnancies, but are upregulated in certain cancers. However, the exact mechanism for this regulation has remained elusive. The aim of this study was to examine the mechanism by which sHLA-G interact with natural killer (NK) cells in vitro. sHLA-G effectively blocked NK lysis of target cells via fracticide killing of NK cells by apoptosis. These studies support the protective role of sHLA-G in immunologic reactions by interacting with NK cells, thus providing a regulatory function.
Lindaman
Amy
A
Department of Internal Medicine, University of Iowa Hospital and Clinics & VA Medical Center Iowa City, 52242, USA.
Dowden
Amy
A
Zavazava
Nicholas
N
D000906:Antibodies / Q000494:pharmacology; D015703:Antigens, CD / Q000276:immunology; D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006680:HLA Antigens / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D015793:HLA-B7 Antigen / Q000494:pharmacology; D059951:HLA-G Antigens; D006367:HeLa Cells; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology
D000906:Antibodies; D015703:Antigens, CD; D006680:HLA Antigens; D015793:HLA-B7 Antigen; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; C105299:LILRB1 protein, human; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D011971:Receptors, Immunologic; D011994:Recombinant Proteins