title: [Molecular basis of partial D phenotypes in Chinese].
abstract: To investigate the molecular basis of partial D phenotypes in Chinese, D variants with weak D expression was screened by using indirect anti-human globulin test (IAT) method, the polymerase chain reaction-sequence specific primer (PCR-SSP) method was employed to amplify RHD specific exons and their flanking regions. The amplification products were sequenced directly to determine the molecular basis of D variants. The results showed that ten cases of partial D phenotypes, including one case of D Va (Kou.), one case of D Va (Hus.), one case of D Va-like (YH.), and seven cases of D VI type III, were detected from 22 cases of weak D phenotype respectively. All ten cases of partial D phenotypes had one RHD allele deleted. In conclusion, the molecular basis of ten cases of partial D phenotype was confirmed, including D Va (Kou.) and D Va-like (YH.) phenotypes reported firstly in Chinese population.
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Wu
authors.forename: Xiao-Zhen
authors.initials: XZ
authors.lastname: Hong
authors.forename: Xian-Guo
authors.initials: XG
authors.lastname: Xu
authors.forename: Ji
authors.initials: J
authors.lastname: He
authors.forename: Qi-Hua
authors.initials: QH
authors.lastname: Fu
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D000483:Alleles; D044466:Asian People; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012204:Rh-Hr Blood-Group System / Q000235:genetics* / Q000276:immunology*
chemical_list: D012204:Rh-Hr Blood-Group System