Analysis of cag-8: a novel poly(Q)-encoding gene in the mouse brain.
We identified 13 transcript isoforms of a trinucleotide-repeat-containing gene, cag-8, that is expressed almost exclusively in the mouse brain. The polypeptide deduced from the cDNA consists of 137 AAs, of which 74 are glutamines. The 130-kb gene is composed of 16 exons, from which at least 13 isoforms with variable UTRs are formed by alternative splicing. A strong positive cis-acting element was found in the neuroblastomaxglioma hybrid NG108-15 and human embryonic kidney HEK293 cell lines. The cag-8 protein was localized in the cytosol and in granular bodies within the nucleus. These findings indicate that cag-8 is a novel poly(Q)-encoding gene, the expression of which is confined primarily to the brain.
Ji
Jin Woo
JW
Department of Life Science, Dongguk University, Seoul 100-715, Republic of Korea.
Yang
Hye Lim
HL
Kim
Sun Jung
SJ
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000502:physiology*; D013347:Subcellular Fractions
C512445:A230006K03Rik protein, mouse; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D010455:Peptides; C097188:polyglutamine