title: Age- and tissue-dependent metallothionein and cytosolic metal distribution in a native Mediterranean fish, Mullus barbatus, from the Eastern Adriatic Sea.
abstract: The levels of metallothionein (MT), a biomarker of metal exposure, and of cytosolic metals (Zn, Cu, Cd), known as MT inducers, were investigated as variables of age (1 to 8 years) and tissue mass (liver, kidney, brain) of red mullet (Mullus barbatus). Within the age from 1 to 8 years the most significant increase is evident for cytosolic Cd in liver (43-fold) and in kidney (5-fold). MT and essential metals are constant with age or slightly increased. Over the growth period, statistically significant MT and metal increase is evident only between 1 and 6-8 years old specimens, while for Cd in liver and kidney cytosol significant increase already exists at 4 years old specimens. Metal distribution in all tissues follows the order: Zn>Cu>Cd, with even 500-800 times lower Cd levels than essential metal levels. Consequently, MTs follow the levels of essential metals, Zn and Cu, indicating MT involvement in homeostasis of essential metals. In contrast to kidney and brain, hepatic MT levels are not age-dependent. Inclusion of hepatic MT measurements and the associated cytosolic metals will be useful in the assessment of long-term metal effects in demersal fish M. barbatus.
authors.affiliation: Division of Marine and Environmental Research, Ruder Bosković Institute, Bijenicka c. 54, P.O. Box 180, HR-10002 Zagreb, Croatia. vfilip@irb.hr
authors.forename: Vlatka Filipović
authors.initials: VF
authors.lastname: Marijić
authors.forename: Biserka
authors.initials: B
authors.lastname: Raspor
mesh_terms: D000375:Aging; D000818:Animals; D001923:Brain Chemistry; D002104:Cadmium / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008668:Metallothionein / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D010473:Perciformes / Q000378:metabolism*; D014018:Tissue Distribution; D015032:Zinc / Q000378:metabolism
chemical_list: D008670:Metals; D002104:Cadmium; D003300:Copper; D008668:Metallothionein; D015032:Zinc