title: A hyaluronidase supercatalyst for the enzymatic polymerization to synthesize glycosaminoglycans.
abstract: Hyaluronidase (HAase) catalyzes multiple enzymatic polymerizations with controlling regio- and stereoselectivity perfectly. This behavior, that is, the single enzyme being effective for multireactions and retaining the enzyme catalytic specificity, is not usual, and hence, HAase is a supercatalyst. Various sugar oxazoline monomers prepared based on the concept "transition-state analogue substrate" were successfully polymerized and copolymerized with HAase catalysis, yielding natural and unnatural glycosaminoglycans.
authors.affiliation: Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan. kobayash@kit.ac.jp
authors.forename: Shiro
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Masashi
authors.initials: M
authors.lastname: Ohmae
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ochiai
authors.forename: Shun-Ichi
authors.initials: S
authors.lastname: Fujikawa
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002384:Catalysis; D006025:Glycosaminoglycans / Q000138:chemical synthesis* / Q000737:chemistry; D006821:Hyaluronoglucosaminidase / Q000737:chemistry*; D008969:Molecular Sequence Data; D013237:Stereoisomerism; D013997:Time Factors
chemical_list: D006025:Glycosaminoglycans; D006821:Hyaluronoglucosaminidase