title: Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues.
abstract: The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.
authors.affiliation: The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia.
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Churchill
authors.forename: Paul R
authors.initials: PR
authors.lastname: Gorry
authors.forename: Daniel
authors.initials: D
authors.lastname: Cowley
authors.forename: Luxshimi
authors.initials: L
authors.lastname: Lal
authors.forename: Secondo
authors.initials: S
authors.lastname: Sonza
authors.forename: Damian F J
authors.initials: DF
authors.lastname: Purcell
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Thompson
authors.forename: Dana
authors.initials: D
authors.lastname: Gabuzda
authors.forename: Justin C
authors.initials: JC
authors.lastname: McArthur
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Pardo
authors.forename: Steven L
authors.initials: SL
authors.lastname: Wesselingh
mesh_terms: D015526:AIDS Dementia Complex / Q000821:virology*; D001253:Astrocytes / Q000821:virology*; D001921:Brain / Q000821:virology*; D002467:Cell Nucleus / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis*; D015497:HIV-1 / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007834:Lasers; D008264:Macrophages / Q000821:virology*; D042282:Microdissection; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000502:physiology*; D016662:Virus Integration
chemical_list: D004279:DNA, Viral