title: A putative acyl-CoA-binding protein is a major phloem sap protein in rice (Oryza sativa L.).
abstract: The N-terminal amino-acid sequence of a major rice phloem-sap protein, named RPP10, was determined. RPP10 is encoded by a single gene in the rice genome. Its complete amino-acid sequence, predicted from the corresponding rice full-length cDNA, showed high similarity to plant acyl-CoA-binding proteins (ACBPs). Western blot analysis using anti-ACBP antiserum revealed that putative ACBP is abundant in the phloem sap of rice plants, and is also present in sieve-tube exudates of winter squash (Cucurbita maxima), oilseed rape (Brassica napus), and coconut palm (Cocos nucifera). These findings give rise to the idea that ACBP may involve lipid metabolism and regulation in the phloem.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Tokyo 113-8657, Japan. suzui.nobuo@jaea.go.jp
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzui
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nakamura
authors.forename: Toru
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hayashi
authors.forename: Tadakatsu
authors.initials: T
authors.lastname: Yoneyama
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D000214:Acyl Coenzyme A; D002352:Carrier Proteins; D010940:Plant Proteins