Polyclonal antibody against a recombinant chlorotoxin-like peptide from the Chinese scorpion and detection of its putative receptors in human glioma cells.
The nucleotide sequence of a type of chlorotoxin-like peptide, an inhibitor of small-conductance Cl(-) channels, from the scorpion, Buthus martensii Karsch, was synthesized (named rBmK CTa) according to the sequence optimized for codon usage in E. coli. It was over-expressed using a pExSecI expression system and purified to homogeneity. Polycolonal antibodies to the purified protein were raised in rats. Overlay assay and pull-down assay showed that this toxin specially binds to two proteins in the glioma cells with corresponding molecular weights of about 80 and 35 kDa. They may serve as candidate receptors or alternative cellular component for interaction with rBmK CTa.
Fu
Yue-Jun
YJ
Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, PR China.
Yin
Li-Tian
LT
Liang
Ai-Hua
AH
D000818:Animals; D000917:Antibody Formation; D015153:Blotting, Western; D045744:Cell Line, Tumor; D005910:Glioma / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051381:Rats; D011955:Receptors, Drug / Q000378:metabolism*; D012604:Scorpion Venoms / Q000276:immunology* / Q000378:metabolism; D012605:Scorpions
C516430:BmK CTa protein, Buthus martensii; D011955:Receptors, Drug; D012604:Scorpion Venoms; D020778:Matrix Metalloproteinase 2