Genetic differences between Tunisian camel and sheep strains of the cestode Echinococcus granulosus revealed by SSCP.
Ovine and dromedary Echinococcus granulosus isolates from Tunisia were identified as G1 and G6 strains based on polymorphism of the mitochondrial cytochrome C oxydase CO1. Single strand conformation polymorphism (SSCP) was used in order to examine the genetic variation within and between Tunisian G1 and G6 strains and to estimate the extent of selfing. The dromedary isolates are genetically distinct from sheep isolates (high value of genetic variation between populations: Fst= 0.46). No significant deficiency in heterozygotes was found in sheep isolates, whereas heterozygote deficiency (suggesting selfing) was found in a limited number of camel isolates.
Oudni-M'rad
M
M
Faculté de Pharmacie, Laboratoire de Parasitologie-Mycologie, Département de Biologie Clinique B, Monastir, Tunisie. mimitos@gnet.tn
Cabaret
J
J
M'rad
S
S
Bouzid
W
W
Mekki
M
M
Belguith
M
M
Sayadi
T
T
Nouri
A
A
Lahmar
S
S
Azaiez
R
R
Mezhoud
H
H
Babba
H
H
D000818:Animals; D002162:Camelus / Q000469:parasitology*; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000145:classification / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D006790:Host-Parasite Interactions; D010802:Phylogeny; D018807:Polymorphism, Single-Stranded Conformational; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D013045:Species Specificity; D014416:Tunisia
D003576:Electron Transport Complex IV