title: Epidermal and dermal characteristics in skin equivalent after systemic and topical application of skin care ingredients.
abstract: Effects of active ingredients from topical and systemic skincare products on structure and organization of epidermis, dermal-epidermal junction (DEJ), and dermis were examined using an in vitro reconstructed skin equivalent (SE). Imedeen Time Perfection (ITP) ingredients (a mixture of BioMarine Complex, grape seed extract, tomato extract, vitamin C) were supplemented systemically into culture medium. Kinetin, an active ingredient from Imedeen Expression Line Control Serum, was applied topically. Both treatments were tested separately or combined. In epidermis, all treatments stimulated keratinocyte proliferation, showing a significant increase of Ki67-positive keratinocytes (P < 0.05). Kinetin showed a twofold increase of Ki67-positive cells, ITP resulted in a fivefold, and ITP+kinetin showed a nine-fold increase. Differentiation of keratinocytes was influenced only by kinetin since filaggrin was found only in kinetin and kinetin+ITP samples. At the DEJ, laminin 5 was slightly increased by all treatments. In dermis, only ITP increased the amount of collagen type I. Both kinetin and ITP stimulated formation of fibrillin-1 and elastin deposition. The effect of kinetin was seen in upper dermis. It stimulated not only the amount of deposited fibrillin-1 and elastin fibers but also their organization perpendicularly to the DEJ. ITP stimulated formation of fibrillin-1 in deeper dermis. In summary, the combination of topical treatment with kinetin and systemic treatment with ITP had complementary beneficial effects in the formation and development of epidermis and dermis.
authors.affiliation: DermData sro, Czech Republic. javi@ferrosan.com
authors.forename: Jana
authors.initials: J
authors.lastname: Vicanova
authors.forename: Charbel
authors.initials: C
authors.lastname: Bouez
authors.forename: Sophie
authors.initials: S
authors.lastname: Lacroix
authors.forename: Lars
authors.initials: L
authors.lastname: Lindmark
authors.forename: Odile
authors.initials: O
authors.lastname: Damour
mesh_terms: D000287:Administration, Topical; D000328:Adult; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology*; D004347:Drug Interactions; D004549:Elastin / Q000378:metabolism; D000078404:Epidermal Cells; D005260:Female; D000071838:Fibrillin-1; D000071837:Fibrillins; D000091344:Filaggrin Proteins; D006025:Glycosaminoglycans / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D007701:Kinetin / Q000494:pharmacology; D008840:Microfilament Proteins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D011506:Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012867:Skin; D017592:Skin Care; D017768:Skin, Artificial; D054392:Adipokines
chemical_list: D004549:Elastin; D000071838:Fibrillin-1; D000071837:Fibrillins; D000091344:Filaggrin Proteins; D006025:Glycosaminoglycans; D007381:Intermediate Filament Proteins; D019394:Ki-67 Antigen; D007701:Kinetin; D008840:Microfilament Proteins; D010937:Plant Growth Regulators; D011506:Proteins; C000605722:FBN1 protein, human; D054392:Adipokines; C000717249:FLG protein, human; C070172:Imedeen