Regioselective esterase-catalyzed feruloylation of L-arabinobiose.
The regioselective chemoenzymatic synthesis of O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1-->5)-L-arabinofuranose has been achieved. The reaction parameters affecting the feruloylation rate and conversion of the enzymatic synthesis, such as the composition of the reaction medium, substrate and enzyme concentration, have been investigated.
Vafiadi
Christina
C
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, GR-15700 Athens, Greece.
Topakas
Evangelos
E
Christakopoulos
Paul
P
D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D002855:Chromatography, Thin Layer; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods; D008958:Models, Molecular; D015394:Molecular Structure; D013237:Stereoisomerism; D013379:Substrate Specificity
D003373:Coumaric Acids; D004187:Disaccharides; C434937:arabinobiose; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase