title: Cloning and characterization of a novel, human cellular retinaldehyde-binding protein CRALBP-like (CRALBPL) gene.
abstract: Cellular retinaldehyde-binding protein (CRALBP) plays a role in the vertebrate visual process as a substrate-routing protein. It belongs to a widespread lipid-binding SEC14-like protein family. All the members of the family have the lipid-binding domain called CRAL-TRIO. Here we have isolated a new human CRAL-TRIO domain containing a CRALBP-like (CRALBPL) gene from the cDNA library of human adult brain. The CRALBPL gene consisted of 1,694 bp and had an ORF encoding putatively 354 amino acids with a CRAL-TRIO domain from 118 to 279 aa. The expression pattern in 18 human tissues indicated that CRALBPL gene was mainly expressed in brain. The alignment of CRAL-TRIO domain showed that CRALBPL had 45% identity with human CRALBP. Subcellular location revealed that CRALBPL protein was located in the cytoplasm of HeLa cells. Western blotting indicated that the CRALBPL had a molecular weight of about 40 kDa.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Kong
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Ye
authors.forename: Kai
authors.initials: K
authors.lastname: Qu
authors.forename: Wen-Qi
authors.initials: WQ
authors.lastname: Pan
authors.forename: Xiang-Hua
authors.initials: XH
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Wan
authors.forename: Jin-Hu
authors.initials: JH
authors.lastname: Guo
authors.forename: Long
authors.initials: L
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: C020932:11-cis-retinal-binding protein; D002352:Carrier Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins