Polymerase chain reaction identification of coagulase-negative Staphylococci and of strain diversity and spread of Staphylococcus epidermidis in a major medical center in Lebanon.
A 2-step polymerase chain reaction (PCR) assay and random amplification of polymorphic DNA (RAPD) analysis, respectively, were assessed to identify coagulase-negative staphylococci organisms to the species level and to determine the strain diversity and spread of Staphylococcus epidermidis, the most frequently isolated species, in a medical center in Beirut, Lebanon. Our data indicated that PCR was faster and was more efficient in identifying S. epidermidis isolates than is conventional biochemical testing. RAPD analysis have shown that S. epidermidis strains were scattered across the different clinical services, demonstrating various clusters of infection in the medical center.
Abdallah
Inaya M
IM
Department of Microbiology and Immunology, American University of Beirut Medical Center, Beirut, Lebanon.
Araj
George F
GF
Matar
Ghassan M
GM
Abdelnour
George
G
Uwaydah
Marwan
M
Abdelnoor
Alexander M
AM
D001483:Base Sequence; D003030:Coagulase / Q000378:metabolism; D017931:DNA Primers; D006801:Humans; D007861:Lebanon; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D013210:Staphylococcus / Q000201:enzymology / Q000302:isolation & purification*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification*
D003030:Coagulase; D017931:DNA Primers