Four new immunomodulating steroidal glycosides from the stems of Stephanotis mucronata.
Four new C-21 steroidal glycosides, mucronatosides E (1), F (2), G (3), and H (4), were isolated from the stems of Stephanotis mucronata. Two of them had the rare aglycone with a double bond between C-6 and C-7. Their structures were elucidated on the basis of spectroscopic data and chemical evidence. These isolated compounds were assayed for their immunological activities in vitro against concanavalin A (Con A)- and lipopolysaccharide (LPS)-induced proliferation of mice splenocytes. Compounds 2 and 4 showed immunosuppressive activities in a dose-dependent manner, while compounds 1 and 3 possessed immunoenhancing activities.
Li
Xiaoyu
X
Department of Chemistry, Zhejiang University, Hangzhou, PR China.
Sun
Hongxiang
H
Ye
Yiping
Y
Chen
Fengyang
F
Tu
Jue
J
Pan
Yuanjiang
Y
D000818:Animals; D029062:Apocynaceae / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007114:Immunization; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008954:Models, Biological; D010936:Plant Extracts / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D006027:Glycosides; D007155:Immunologic Factors; D010936:Plant Extracts; D013256:Steroids