Volatilisation of metals and metalloids by the microbial population of an alluvial soil.
In order to assess the microbial contribution to the volatilisation of metal(loid)s by methylation and hydridisation in the environment, we focused on soils of different origin. Here, we describe the biogenic production of volatile metal(loid) species of an alluvial soil with rather low metal(loid) contamination. The production of volatile metal(loid) compounds was monitored in soil suspensions kept under anaerobic conditions over an incubation time of 3 months. In the headspace of the samples, we detected mainly hydrids and methylated derivatives of a broad variety of elements such as arsenic, antimony, bismuth, selenium, tellurium, mercury, tin and lead, with the volatile products of arsenic, antimony and selenium representing the highest portions. Classical cultivation-dependent procedures resulted in the isolation of a strictly anaerobic Gram-positive strain (ASI-1), which shows a high versatility in transforming metal(loid) ions to volatile derivatives. Strain ASI-1 is affiliated to the species Clostridium glycolicum due to its high 16S rDNA sequence similarity with members of that species. As shown by fluorescence in situ hybridisation, strain ASI-1 amounts to approximately 2% of the total microbial flora of the alluvial soil. Since the spectrum of volatile metal(loid) compounds produced by this strain is very similar to that obtained by the whole population regarding both the broad variety of metal(loid)s converted and the preference for volatilising arsenic, antimony and selenium, we suggest that this strain may represent a dominant member of the metal(loid) volatilisating population in this habitat.
Meyer
Jörg
J
Fachbereich Biologie und Geografie, Fachgebiet Mikrobiologie, Universität Duisburg-Essen, Campus Essen, Universitätsstr. 5, 45117 Essen, Germany.
Schmidt
Annette
A
Michalke
Klaus
K
Hensel
Reinhard
R
D000693:Anaerobiosis; D003013:Clostridium / Q000145:classification / Q000166:cytology / Q000302:isolation & purification / Q000378:metabolism*; D005858:Germany; D008670:Metals / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D014835:Volatilization
D008670:Metals; D012336:RNA, Ribosomal, 16S; D012987:Soil