title: Neural differentiation of embryonic stem cells induced by conditioned medium from neural stem cell.
abstract: Embryonic stem cells can proliferate indefinitely and are capable of differentiating into derivatives of all three embryonic germ layers in vitro, including the neural lineage. The main objective of this study is to test the effects of neural stem cell conditioned medium on the neural differentiation of mouse embryonic stem cells. When cultured in neural stem cell conditioned medium, mouse embryonic stem cells can form floating cell spheres composed of many nestin-positive cells. After trypsinization and growth on gelatin, these embryonic stem cell-derived neural progenitor cells can be expanded for more than 3 months without loss of neural progenitor characteristics. Both neuronal and glial cells can be readily generated from these cells under differentiation conditions. Thus, neural stem cell conditioned medium is a highly potent reagent for inducing the development of mouse embryonic stem cells into the neural lineage, especially neural progenitor cells.
authors.affiliation: Centre for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, Guangzhou, Guangdong, China.
authors.forename: Jia-Qing
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Xin-Bing
authors.initials: XB
authors.lastname: Yu
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Ma
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Yu
authors.forename: Ai-Xia
authors.initials: AX
authors.lastname: Zhang
authors.forename: Guo
authors.initials: G
authors.lastname: Huang
authors.forename: Frank Fuxiang
authors.initials: FF
authors.lastname: Mao
authors.forename: Xiu-Ming
authors.initials: XM
authors.lastname: Zhang
authors.forename: Zhi-Chong
authors.initials: ZC
authors.lastname: Wang
authors.forename: Shu-Nong
authors.initials: SN
authors.lastname: Li
authors.forename: Bruce T
authors.initials: BT
authors.lastname: Lahn
authors.forename: Andy Peng
authors.initials: AP
authors.lastname: Xiang
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004622:Embryo, Mammalian; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin; D009457:Neuroglia / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
chemical_list: D017077:Culture Media, Conditioned; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D012333:RNA, Messenger