PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae.
Streptococcus phocae, a bacterial pathogen of seals, could reliably be identified by PCR amplification using oligonucleotide primers designed according to species specific segments of the previously sequenced 16S rRNA gene and the 16S-23S rDNA intergenic spacer region of this species. The PCR mediated assay allowed an identification of S. phocae isolated from harbor and gray seals and from Atlantic salmons. No cross-reaction could be observed with 13 different other streptococcal species and subspecies and with Lactococcus garvieae strains investigated for control purposes.
Hassan
A A
AA
Institut für Tierärztliche Nahrungsmittelkunde, Professur für Milchwissenschaften, Justus-Liebig-Universität Giessen, Ludwigstr. 21, 35390 Giessen, Germany.
Vossen
A
A
Lämmler
C
C
Siebert
U
U
Fernández-Garayzábal
J F
JF
D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D020317:Salmo salar; D046048:Seals, Earless / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013290:Streptococcal Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S