title: The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved synteny with Medicago truncatula.
abstract: We report the first gene-based linkage map of Lupinus angustifolius (narrow-leafed lupin) and its comparison to the partially sequenced genome of Medicago truncatula. The map comprises 382 loci in 20 major linkage groups, two triplets, three pairs and 11 unlinked loci and is 1,846 cM in length. The map was generated from the segregation of 163 RFLP markers, 135 gene-based PCR markers, 75 AFLP and 4 AFLP-derived SCAR markers in a mapping population of 93 recombinant inbred lines, derived from a cross between domesticated and wild-type parents. This enabled the mapping of five major genes controlling key domestication traits in L. angustifolius. Using marker sequence data, the L. angustifolius genetic map was compared to the partially completed M. truncatula genome sequence. We found evidence of conserved synteny in some regions of the genome despite the wide evolutionary distance between these legume species. We also found new evidence of widespread duplication within the L. angustifolius genome.
authors.affiliation: School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, Perth, WA 6009, Australia.
authors.forename: Matthew N
authors.initials: MN
authors.lastname: Nelson
authors.forename: Huyen T T
authors.initials: HT
authors.lastname: Phan
authors.forename: Simon R
authors.initials: SR
authors.lastname: Ellwood
authors.forename: Paula M
authors.initials: PM
authors.lastname: Moolhuijzen
authors.forename: James
authors.initials: J
authors.lastname: Hane
authors.forename: Angela
authors.initials: A
authors.lastname: Williams
authors.forename: Clare E
authors.initials: CE
authors.lastname: O'Lone
authors.forename: John
authors.initials: J
authors.lastname: Fosu-Nyarko
authors.forename: Marie
authors.initials: M
authors.lastname: Scobie
authors.forename: Mehmet
authors.initials: M
authors.lastname: Cakir
authors.forename: Michael G K
authors.initials: MG
authors.lastname: Jones
authors.forename: Matthew
authors.initials: M
authors.lastname: Bellgard
authors.forename: Michał
authors.initials: M
authors.lastname: Ksiazkiewicz
authors.forename: Bogdan
authors.initials: B
authors.lastname: Wolko
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Barker
authors.forename: Richard P
authors.initials: RP
authors.lastname: Oliver
authors.forename: Wallace A
authors.initials: WA
authors.lastname: Cowling
mesh_terms: D000483:Alleles; D005787:Gene Frequency; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D031297:Lupinus / Q000235:genetics*; D029902:Medicago / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D005819:Genetic Markers