[Killing activity in DC and CIK co-culture against hepatocarcinoma cells].
This study was aimed to investigate the proliferation activities and phenotype changes of DC, CIK and DC-CIK, and their cytotoxicity against hepatocarcinoma cells in co-culture of DC with CIK. Peripheral blood mononuclear cells (PBMNC) were isolated from healthy adult donors. After incubation of PBMNC for 2 hours, DCs were induced from adherent cells by some cytokines and CIKs were generated from non-adherent cells. Mature DCs were harvested after incubation for 9 days, and then were co-cultured with CIK at ratio of 1:5 for 3 days. The cytotoxicity activity against SMMC-7721 hepatocellular carcinoma cell line was detected by MTT assay. The results showed that CIK cells were able to lyse SMMC-7721 hepatocellular carcinoma cells at low ratios of effector to target. This effect was significantly enhanced by co-culture with DCs. It is concluded that CIK cells have high lytic activity against 7721 hepatocellular carcinoma cell line, which can be enhanced by co-culture with DC. DC-CIK cells are highly effective immune cells.
Chen
Bao-An
BA
Department of Hematology, Zhongda Hospital, Southeast University, Nanjing 210009, China. cba8888@hotmail.com
Li
Man
M
Sun
Zai-Yang
ZY
Li
Cui-Ping
CP
Gao
Chong
C
Sun
Yun-Yu
YY
D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000473:pathology; D002478:Cells, Cultured; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology / Q000276:immunology*; D008113:Liver Neoplasms / Q000276:immunology* / Q000473:pathology