No evidence of significant silencing of Fanconi genes FANCF and FANCB or Nijmegen breakage syndrome gene NBS1 by DNA hyper-methylation in sporadic childhood leukaemia.
Fanconi anaemia (FA) and Nijmegen breakage syndrome (NBS) carry a high risk of haematological cancer. Affected cellular pathways may be modulated in sporadic malignancies and silencing of FANCF through methylation has been shown to cause somatic disruption of the FA pathway. Combined bisulphite restriction analysis for methylation of FANCF, FANCB and NBS1 was used to investigate 81 sporadic acute childhood leukaemias. No methylation was detected at any associated CpG sites analysed. This does not exclude very low levels of FANCF, FANCB or NBS1 methylation, but suggests other factors are responsible for chemo-sensitivity and chromosomal instability in sporadic childhood leukaemia.
Meyer
Stefan
S
Department of Paediatric Haematology and Oncology, Central Manchester and Manchester Children's University Hospitals NHS Trust, Manchester, UK. stefan.meyer-2@manchester.ac.uk
White
Daniel J
DJ
Will
A M
AM
Eden
Tim
T
Sim
Alyson
A
Brown
Robert
R
Strathdee
Gordon
G
D000208:Acute Disease; D000293:Adolescent; D018797:Cell Cycle Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D043171:Chromosomal Instability; D018899:CpG Islands; D019175:DNA Methylation; D052219:Fanconi Anemia Complementation Group F Protein / Q000235:genetics; D051856:Fanconi Anemia Complementation Group Proteins / Q000235:genetics*; D052138:Genes, Neoplasm / Q000235:genetics; D006801:Humans; D007223:Infant; D007938:Leukemia / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics
D018797:Cell Cycle Proteins; D052219:Fanconi Anemia Complementation Group F Protein; D051856:Fanconi Anemia Complementation Group Proteins; C112156:NBN protein, human; D009687:Nuclear Proteins