The intradermal Leishmanin reaction induces antigen-specific maturation of canine dendritic cells with up-regulation of MHCII synthesis and expression.
Dendritic cells (DCs) are professional antigen-presenting cells that reside in many tissues, including the skin. This study showed that intradermal injection of leishmanin in Leishmania infantum-infected dogs induced the "up-regulation" of surface MHCII expression, associated with progressive ultrastrucutural changes characteristic of DC maturation, including the formation of multilaminar MHC class II-containing compartments and arrays of tubulo-vesicular structures. These changes were not observed in control dogs from L. infantum non-endemic areas. The results indicated that canine DCs were effector cells in delayed-type hypersensitivity, that the leishmanin reaction was specific for a cell-mediated reaction to L. infantum in infected dogs, and that canine DCs possessed ultrastructural organelles reminiscent of those in activated human DCs.
Sacchi
L
L
Department of Animal Biology, University of Pavia, Italy.
Calvi
L E
LE
Kramer
L H
LH
Ferroglio
E
E
Grandi
G
G
Clementi
E
E
Corona
S
S
D000818:Animals; D000941:Antigens / Q000276:immunology / Q000378:metabolism*; D000953:Antigens, Protozoan / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism* / Q000469:parasitology / Q000648:ultrastructure; D004285:Dogs; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000502:physiology* / Q000648:ultrastructure; D018314:Leishmania infantum / Q000276:immunology; D015854:Up-Regulation
D000941:Antigens; D000953:Antigens, Protozoan; D000949:Histocompatibility Antigens Class II; C032127:leishmanin