[Development of an immunochromayography assay method for the detection of Yersinia pestis].
OBJECTIVE
To develop a method of immunochromatography assay (ICA) with sensitive, specific, rapid, simple and suitable for the detection of Yersinia pestis antigen at the local laboratories.


METHODS
Colloidal gold labeled with the anti-F1 antibody of Yersinia pestis, was connnected with the anti-F1 antibody of Yersinia pestis to pyroxylin membrane and assembled them to the dipstick of ICA.


RESULTS
Results showed that the rates of sensitivity for F1 antigen and Yersinia pestis were 1 ng/ml and 1.56 x 10(5) CFU/ml respectively. However, Yersinia pseudotuberculosis et al could not be detected by dipstick of ICA.


CONCLUSIONS
The method of ICA appeared to be consistent to those of r-IHA with better specificity and sensitivity but was simple and rapid for the detection of Yersinia pestis and F1 antigen.
Zhu
Hong
H
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Zhang
Chun-hua
CH
Tan
Hua
H
He
Jun
J
Zhao
Bin
B
Duan
Qing
Q
D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods; D012680:Sensitivity and Specificity; D015010:Yersinia pestis / Q000276:immunology*
D001426:Bacterial Proteins; C086725:caf1 protein, Yersinia pestis