The structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O36 containing 3-deoxy-d-manno-oct-2-ulosonic acid.
An oligosaccharide that corresponds to the repeating unit of the O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O36. Structural studies of the oligosaccharide and O-deacylated lipopolysaccharide were performed using sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, and H-detected (1)H,(13)C HSQC and HMBC experiments. It was found that the O-polysaccharide is built up of linear trisaccharide repeating units containing 2-acetamido-2-deoxyglucose, 6-deoxy-l-talose (l-6dTal), and 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) and has the following structure. [structure: see text]
Kocharova
Nina A
NA
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Ovchinnikova
Olga G
OG
Torzewska
Agnieszka
A
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
Rozalski
Antoni
A
D000117:Acetylglucosamine / Q000032:analysis; D002240:Carbohydrate Sequence; D003837:Deoxy Sugars / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D011532:Providencia / Q000737:chemistry*; D013400:Sugar Acids / Q000032:analysis*
D003837:Deoxy Sugars; D006601:Hexoses; D019081:O Antigens; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; C011754:6-deoxytalose; D000117:Acetylglucosamine