title: Distribution of Epstein-Barr virus genotypes in throat washings, sera, peripheral blood lymphocytes and in EBV positive tumor biopsies from Slovenian patients with nasopharyngeal carcinoma.
abstract: Nasopharyngeal carcinoma (NPC) is a malignancy closely associated with Epstein-Barr virus (EBV). It is prevalent among the Chinese of Southern China, whereas outside China, the position seems to be different. The aim of this study was to determine the distribution of EBV genotypes in the patients with NPC in Slovenia, which is a nonendemic area. Detection of EBV was undertaken by testing the throat washes, sera, peripheral blood lymphocytes (PBLs), and biopsies of primary tumors of 48 patients with NPC in Slovenia. The sera of 20 patients with serologically confirmed primary EBV infection served as a control clinical material. The analysis of genotypes was carried out on three regions of EBV genome; BamHI WYH, BamHI I, and BamHI F, using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The results show that, in Slovenia, the predominant combination of EBV genotypes based on the differences in the three genomic regions is ADF. This combination was found in 56 out of 103 different EBV positive clinical samples (throat washes, sera, PBLs, and tumor biopsies) of patients with NPC and in 15 out of 17 sera of patients with primary EBV infection. Very low number of genotypes C and f were detected, in spite of the fact that these two genotypes were considered to be associated with the development and/or maintenance of NPC in Southern China. Genotype f was found in only two tumor biopsies; in all other clinical samples (throat washes, sera and PBLs), genotype F was detected. Genotype C was proven in 31/103 clinical samples, with the highest percentage in tumor biopsies (37.5%). As in the NPC patients from other countries (Alaska is an exception), genotype A was predominant and was detected in 86/103 clinical samples. Genotype B was found in 15 clinical samples of patients with NPC and in 3 the two genotypes A and B were found. In comparison to China, these results show different EBV genotypes distribution. It seems that the genetic disposition of human population is an important factor that may contribute to different susceptibility for specific EBV genotypes.
authors.affiliation: Institute of Microbiology and Immunology, Medical Faculty, Ljubljana, Slovenia. polona.klemenc@mf.uni-lj.se
authors.forename: P
authors.initials: P
authors.lastname: Klemenc
authors.forename: J
authors.initials: J
authors.lastname: Marin
authors.forename: E
authors.initials: E
authors.lastname: Soba
authors.forename: N
authors.initials: N
authors.lastname: Gale
authors.forename: S
authors.initials: S
authors.lastname: Koren
authors.forename: P
authors.initials: P
authors.lastname: Strojan
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000453:epidemiology; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008214:Lymphocytes / Q000821:virology*; D009303:Nasopharyngeal Neoplasms / Q000453:epidemiology* / Q000821:virology*; D017524:Slovenia / Q000453:epidemiology
chemical_list: D004279:DNA, Viral