title: Dynamics of dendritic cell migration and the subsequent induction of protective immunity in the lung after repeated airway challenges by nontypeable Haemophilus influenzae outer membrane protein.
abstract: To determine the dynamics of dendritic cell (DCs) migration and their role in recurrent infections by nontypeable Haemophilus influenzae (NTHi), the migration of mature DC into pulmonary lymph nodes (LN) and the development of a P6-specific immune response and bacterial clearance in the lung were examined after repeated airway challenges with outer membrane protein (OMP) at 1-week intervals in mice. Although the migration of mature DC into the pulmonary LN is attenuated after repeated airway challenge with OMP, the in vitro P6-specific T cell proliferation in the cultured pulmonary LN cells was enhanced and was subsequently linked to the development of P6-specific IgA production and the development of protective immunity in the airway of mice.
authors.affiliation: Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kurita
authors.forename: Jun
authors.initials: J
authors.lastname: Koyama
authors.forename: Shozaburo
authors.initials: S
authors.lastname: Onizuka
authors.forename: Kazushi
authors.initials: K
authors.lastname: Motomura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Kiwao
authors.initials: K
authors.lastname: Watanabe
authors.forename: Masachika
authors.initials: M
authors.lastname: Senba
authors.forename: Michael A
authors.initials: MA
authors.lastname: Apicella
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Murphy
authors.forename: Horoyuki
authors.initials: H
authors.lastname: Yoneyama
authors.forename: Kouji
authors.initials: K
authors.lastname: Matsushima
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nagatake
authors.forename: Kazunori
authors.initials: K
authors.lastname: Oishi
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D006192:Haemophilus Infections / Q000276:immunology* / Q000517:prevention & control; D018073:Haemophilus Vaccines / Q000276:immunology*; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000276:immunology*; D051379:Mice
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018073:Haemophilus Vaccines