Delivery of antibody-captured proteins into living cells using PTD-fused protein A.
Protein transduction domain (PTD)-mediated protein delivery into animal cells is a useful technique for regulating cellular functions. Proteins captured by antibodies were delivered into living cells using an antibody/PTD-fused protein A complex. As a model protein, fluorescent-modified antibodies, captured by their respective primary antibody, were analyzed by fluorescence-activated cell sorting (FACS) which showed that the fluorescent-modified antibodies were directly delivered into cells. Peroxidase, captured by its specific antibody, was also delivered into cells and retained its activity.
Mie
Masayasu
M
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.
Mori
Kazuto
K
Funabashi
Hisakage
H
Kobatake
Eiry
E
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity / Q000502:physiology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology; D005434:Flow Cytometry; D015696:Gene Products, tat / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D051379:Mice; D010544:Peroxidases / Q000378:metabolism / Q000493:pharmacokinetics; D021381:Protein Transport / Q000502:physiology*; D013205:Staphylococcal Protein A / Q000737:chemistry*
D000906:Antibodies; D015696:Gene Products, tat; D013205:Staphylococcal Protein A; D010544:Peroxidases