Primary amine coding as a path to comparative proteomics.
Various isotope coding strategies are being used today in the field of comparative proteomics. This article specifically reviews the strengths and limitations of various N-termini-directing strategies. N-termini-directed coding strategy allows for use of different chromatographic enrichment techniques. Since N-termini-directed coding strategies are global in nature, they can be utilized in studying PTMs as well as protein expression. Using different N-termini-directed coding strategies, both relative and absolute quantification of proteins can be achieved either in the MS mode or in the MS/MS mode. The review ends with the conclusion that significant improvements have been made in the last decade. Among various issues, a need still exists for a better understanding of the kinetic issues in proteomics, relative protein pool sizes for different proteins and the issue of stimulus-induced changes in protein aggregation. Another critical issue that needs to be addressed in great detail is the role of PTMs in regulation.
Regnier
Fred E
FE
Department of Chemistry, Purdue University, West Lafayette, IN 47906, USA. fregnier@purdue.edu
Julka
Samir
S
D000588:Amines / Q000737:chemistry*; D015415:Biomarkers / Q000737:chemistry*; D006031:Glycosylation; D007554:Isotopes / Q000737:chemistry; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods* / Q000639:trends*
D000588:Amines; D015415:Biomarkers; D007554:Isotopes; D011506:Proteins