title: [Evaluation of two methods for counting residual white blood cells in thrombocytaphoresis concentrates].
abstract: To evaluate flow-cytometry and Nageotte method for counting residual WBC in thrombocytaphoresis concentrates, their accuracies were determined by dilution studies separately; the repeatability was determined by measuring the interassay coefficient of variation for 14 replicates of a sample with known leukocyte concentration. 102 samples of leukocyte-depleted thrombocytaphoresis concentrates were detected by the above mentioned two methods, and the results were compared each other. The results showed that no difference was observed between two methods over a range of leukocyte concentrations from 0.8 to 10 WBC/microl (P > 0.05). In conclusion, flow-cytometry and Nageotte methods can be used for quality control of WBC-reduced blood components.
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou 310006, China. wyjygm@yahoo.com.cn
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Zhuo-Lan
authors.initials: ZL
authors.lastname: Sheng
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Bo-Li
authors.initials: BL
authors.lastname: Huang
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Hu
authors.forename: Wu-Wei
authors.initials: WW
authors.lastname: Feng
mesh_terms: D016913:Blood Component Transfusion; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*; D047589:Leukocyte Reduction Procedures / Q000379:methods; D010983:Plateletpheresis