title: [Effects of magnetic resonance imaging on neutrophil functions].
abstract: We evaluated the effects of magnetic resonance (MR) imaging on human neutrophil functions. In addition of the counting of leukocyte and neutrophil number, expression levels of adhesion molecules on neutrophil surface such as CD11b and L-selectin, and reactive oxygen species (ROS) production were determined by means of flowcytometry. Complete blood count did not show any difference between before and after MR imaging in five normal healthy volunteers. The levels of cell surface adhesion molecules were not altered in both in vivo MR imaging (n=5) and in vitro MR exposure experiments (n=13). Moreover, the levels of ROS production were also not affected by in vivo MR imaging. On the other hand, neutrophils exposed to MR in vitro exhibited significant increase in ROS production after stimulation with fMLP combined with lipopolysaccharide, although no increase was observed with PMA stimulation. Actually, there have been no reports describing the complication relating to hyper-neutrophil function as far as we could search, but it might be necessary to evaluate the biological effects of MR imaging especially under the pathologic circumstances that induce neutrophil activation.
authors.affiliation: Blood Transfusion Division, Kobe University Hospital, Kobe 650-0017.
authors.forename: Katsuyasu
authors.initials: K
authors.lastname: Saigo
authors.forename: Yukie
authors.initials: Y
authors.lastname: Jyokei
authors.forename: Makoto
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Manami
authors.initials: M
authors.lastname: Kishimoto
authors.forename: Kyoko
authors.initials: K
authors.lastname: Matsunaga
authors.forename: Mariko
authors.initials: M
authors.lastname: Takenokuchi
authors.forename: Kosuke
authors.initials: K
authors.lastname: Sakurai
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kawamitsu
authors.forename: Masahiko
authors.initials: M
authors.lastname: Fujii
authors.forename: Shunichi
authors.initials: S
authors.lastname: Kumagai
mesh_terms: D000328:Adult; D015815:Cell Adhesion Molecules / Q000032:analysis; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D017382:Reactive Oxygen Species