Temperature modifies gene expression in subcuticular epithelial cells of white spot syndrome virus-infected Litopenaeus vannamei.
Subtractive suppressive hybridization was used to identify differentially expressed genes in subcuticular tissues from white spot syndrome virus(WSSV)-infected shrimp kept at different temperatures. Subtractive libraries I and II contained genes expressed at 26 and 33 degrees C, respectively. Three hundred and seventy-nine insert positive clones were selected to confirm differential expression by dot-blot hybridization. Twenty-two clones from library I and eight from library II were sequenced. All sequences from Library I corresponded to white spot syndrome virus genes. From library II, five clones were homologous with previously reported expressed sequence tags of Litopenaeus vannamei, two had similarity with beta-actin and one transcript represented an unknown gene. Over-expression of VP15 in shrimp at 26 degrees C was further confirmed by real-time polymerase chain reaction (PCR), whereas beta-actin expression was similar in animals kept at both temperatures. Together, our results show that hyperthermia reduces the expression of WSSV genes on shrimp subcuticular epithelial cells.
Reyes
Alejandro
A
Corporación Corpogen, Molecular Biotechnology Group, Bogotá, Colombia. ale-reye@spymac.com
Salazar
Marcela
M
Granja
Clarissa
C
D000818:Animals; D004847:Epithelial Cells / Q000276:immunology / Q000821:virology*; D020869:Gene Expression Profiling / Q000379:methods*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D015723:Gene Library; D006358:Hot Temperature; D033561:Penaeidae / Q000235:genetics / Q000821:virology*; D016133:Polymerase Chain Reaction; D046848:White spot syndrome virus 1 / Q000235:genetics* / Q000276:immunology