title: A dataset of human fetal liver proteome identified by subcellular fractionation and multiple protein separation and identification technology.
abstract: A high throughput process including subcellular fractionation and multiple protein separation and identification technology allowed us to establish the protein expression profile of human fetal liver, which was composed of at least 2,495 distinct proteins and 568 non-isoform groups identified from 64,960 peptides and 24,454 distinct peptides. In addition to the basic protein identification mentioned above, the MS data were used for complementary identification and novel protein mining. By doing the analysis with integrated protein, expressed sequence tag, and genome datasets, 223 proteins and 15 peptides were complementarily identified with high quality MS/MS data.
authors.affiliation: Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing Proteomics Research Center, 27 Taiping Road, Beijing 100850, China.
authors.forename: Wantao
authors.initials: W
authors.lastname: Ying
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Lihai
authors.initials: L
authors.lastname: Guo
authors.forename: Yunwei
authors.initials: Y
authors.lastname: Hao
authors.forename: Yangjun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Songfeng
authors.initials: S
authors.lastname: Wu
authors.forename: Fan
authors.initials: F
authors.lastname: Zhong
authors.forename: Jinglan
authors.initials: J
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Shi
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Wan
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Li
authors.forename: Handong
authors.initials: H
authors.lastname: Wei
authors.forename: Jianqi
authors.initials: J
authors.lastname: Li
authors.forename: Zhongsheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Xue
authors.forename: Yun
authors.initials: Y
authors.lastname: Cai
authors.forename: Yunping
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Qian
authors.forename: Fuchu
authors.initials: F
authors.lastname: He
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002853:Chromatography, Liquid; D019295:Computational Biology; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D020539:Sequence Analysis, Protein; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013347:Subcellular Fractions
chemical_list: D011506:Proteins; D020543:Proteome