The effects of impact and non-impact exercise on circulating markers of collagen remodelling in humans.
Exercise has been shown to influence collagen synthesis and degradation. The aim of this study was to determine whether the stimulus for these changes is metabolic or mechanical. Eight healthy adults aged 22 +/- 4 years (mean +/- s) completed two exercise bouts-a 10 km road run and a deep water running session. Blood samples were collected before exercise and on days 1, 2, 3, 6 and 10 after exercise for measurement of creatine kinase activity, type IV collagen antigenicity, and concentrations of matrix metalloproteinase (MMP)- 9, tissue inhibitors of metalloproteinase (TIMP)- 1 and -2, and the MMP-2/TIMP-2 complex. Serum creatine kinase was elevated 24 h after the road run, but unchanged after the deep water running session. Serum collagen IV antigenicity decreased after both the road run and the deep water running session, suggesting suppressed type IV collagen synthesis in response to exercise, although serum MMPs and TIMPs remained unchanged after exercise. These results suggest that collagen IV synthesis is temporarily suppressed after exercise, irrespective of exercise type.
Mackey
Abigail L
AL
Department of Physical Education and Sport Sciences, University of Limerick, Limerick, Ireland.
Donnelly
Alan E
AE
Swanton
Alan
A
Murray
Fiona
F
Turpeenniemi-Hujanen
Taina
T
D000328:Adult; D015415:Biomarkers / Q000097:blood; D024141:Collagen Type IV / Q000276:immunology*; D003402:Creatine Kinase / Q000097:blood; D000939:Epitopes / Q000097:blood; D005260:Female; D006801:Humans; D007101:Immersion; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D012420:Running / Q000502:physiology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000097:blood*
D015415:Biomarkers; D024141:Collagen Type IV; D000939:Epitopes; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D003402:Creatine Kinase; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9