Interactions of carbohydrates and proteins by fluorophore-assisted carbohydrate electrophoresis.
A sensitive,specific, and rapid method for the detection of carbohydrate-protein interactions is demonstrated by fluorophore-assisted carbohydrate electrophoresis (FACE). The procedure is simple and the cost is low. The advantage of this method is that carbohydrate-protein interactions can be easily displayed by FACE, and the carbohydrates do not need to be purified.
Huang
Gang-Liang
GL
State Key Laboratory of Microbial Technology,Shandong University,Jinan,Shandong 250100, People's Republic of China. hgl226@126.com
Mei
Xin-Ya
XY
Wang
Peng-George
PG
D002241:Carbohydrates; D004586:Electrophoresis / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D009281:Naphthalenes / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012680:Sensitivity and Specificity
D002241:Carbohydrates; D005456:Fluorescent Dyes; D009281:Naphthalenes; D011506:Proteins; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid