title: Extensive analysis of different allelelic structures of the chicken BF2 and beta2m proteins.
abstract: No information is available to date on the different allelelic structures of the chicken MHC class I (BF2) and beta2m proteins. To elucidate the structure, new allelic beta2m and five different BF2 genes were expressed solubly and purified in a pMAL-p2X/E. coli TB1 system. The 2D structure was detected by circular dichroism (CD) spectroscopy, and the 3D structures of their peptide-binding domain (PBD) were analyzed by homology modeling. The sequence lengths of the alpha-helix, beta-sheet, turn, and random coil in the five BF2 proteins were 69-73 aa, 67-72 aa, 35-37 aa, and 94-98 aa, respectively. The new beta2m protein displayed a typical beta-sheet. Homology modeling of the different BF2 and beta2m proteins demonstrated similarities to the structure of human and rat MHC class I proteins. The 3D structure, however, revealed that the BF2 and beta2m structures were unique. The correct refolding of recombinant BF2 and beta2m proteins might be a powerful tool to further detect antigenic peptides.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, P.R. China.
authors.forename: Xin Sheng
authors.initials: XS
authors.lastname: Li
authors.forename: Qin Mei
authors.initials: QM
authors.lastname: Fang
authors.forename: Ruo Qian
authors.initials: RQ
authors.lastname: Yan
authors.forename: Feng Shan
authors.initials: FS
authors.lastname: Gao
authors.forename: Hui Fang
authors.initials: HF
authors.lastname: Hao
authors.forename: Zhen Hu
authors.initials: ZH
authors.lastname: Jia
authors.forename: Chang You
authors.initials: CY
authors.lastname: Lin
authors.forename: Chun
authors.initials: C
authors.lastname: Xia
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D002942:Circular Dichroism / Q000662:veterinary; D004247:DNA / Q000737:chemistry / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D017422:Sequence Analysis, DNA; D001613:beta 2-Microglobulin / Q000235:genetics* / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I; D011994:Recombinant Proteins; D001613:beta 2-Microglobulin; D004247:DNA