title: Urinary concentrations of bisphenol A in relation to biomarkers of sensitivity and effect and endocrine-related health effects.
abstract: The impact of endocrine-disrupting chemicals (EDCs) on human health is not yet clear because of difficulties in ascertaining their biological effects. In the present study, we evaluated exposure to the EDC, bisphenol A (BPA), in 172 Koreans in relation to biomarkers of susceptibility and effect. The subjects completed questionnaires, which documented occupation, education, lifestyle factors, potential sources of BPA-exposure, and the occurrence of self-diagnosed endocrine disorders. None of the subjects were occupationally exposed to BPA; however, urinary levels of conjugated BPA, determined by HPLC/FD, ranged from 0.03-62.4 microg/l (median, 7.86). The frequencies of potential susceptibility biomarkers, the UGT1A6-Arg184Ser and the SULT1A1-Arg213His polymorphisms, were not associated with urinary BPA levels, either as single genes or in combination. Indirect effects of BPA exposure on the susceptibility to mutagens were evaluated by comparing urinary BPA concentrations with MNNG-induced sister-chromatid exchange (SCE) in lymphocytes cultured from the subjects. BPA exposure showed marginal or significant associations with theSCEs induced by the low doses of MNNG (0-0.4 mM). However, there was no overall association between urinary BPA levels and MNNG-induced frequency at doses ranging from 0.2-0.6 mM. Finally, we did not detect an association between urinary BPA concentration and endocrine-related disorders. Even though we were unable to find a strong association between BPA exposure and a biological response, possibly because of the limited number of subjects, we observed that most of the subjects were exposed to BPA. Therefore, continuous biological monitoring of BPA is a prudent measure to prevent possible BPA-related health risks.
authors.affiliation: Department of Toxicology, College of Pharmacy, Sookmyung Women's University, Yongsan-Gu, Seoul 140-742, Republic of Korea. myang@sookmyung.ac.kr
authors.forename: Mihi
authors.initials: M
authors.lastname: Yang
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Seong-Sil
authors.initials: SS
authors.lastname: Chang
authors.forename: In-Seon
authors.initials: IS
authors.lastname: Lee
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kawamoto
mesh_terms: D000328:Adult; D015239:Arylsulfotransferase / Q000235:genetics; D001559:Benzhydryl Compounds; D015415:Biomarkers / Q000652:urine; D001773:Blood Cells / Q000187:drug effects; D002478:Cells, Cultured; D052244:Endocrine Disruptors / Q000652:urine*; D004700:Endocrine System Diseases / Q000139:chemically induced / Q000235:genetics; D004781:Environmental Exposure; D005260:Female; D020022:Genetic Predisposition to Disease; D014453:Glucuronosyltransferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000009:adverse effects; D008875:Middle Aged; D010636:Phenols / Q000009:adverse effects / Q000652:urine*; D011110:Polymorphism, Genetic; D012854:Sister Chromatid Exchange; D015238:Sulfotransferases / Q000235:genetics*; D000098826:Bisphenol A Compounds
chemical_list: D015239:Arylsulfotransferase; D001559:Benzhydryl Compounds; D015415:Biomarkers; D052244:Endocrine Disruptors; D014453:Glucuronosyltransferase; D008769:Methylnitronitrosoguanidine; D010636:Phenols; D015238:Sulfotransferases; C110655:UDP-glucuronosyltransferase, UGT1A6; C117544:SULT1A1 protein, human; C006780:bisphenol A; D000098826:Bisphenol A Compounds