Differentiation of human amniotic membrane cells into osteoblasts in vitro.
OBJECTIVE
Pluripotent stem cells may be exist in the human amniotic membrane (HAM). The present article was aimed at establishing HAM cell lines and investigating their differentiation into osteoblasts in vitro.


METHODS
HAM cell lineswere established using routine cell culture techniques and expanded in vitro.


RESULTS
HAM cells were propagated from 45 out of 50 specimens (90%) and all were maintained normal karyotypes in vitro. Alkaline phosphatase (ALP) activity and alizarin red S stain were positive for 12 out of 22 HAM cell lines (54.5%). The 22 HAM cell lines were selectedat random.


CONCLUSIONS
This study demonstrates that this isolation method II was more effective for establishing cell lines which differentiate into osteoblast rather than method I.
Tamagawa
Tomoharu
T
Instute of Cell Biology, Ishiwata Hospital, Ibaraki, Japan.
Ishiwata
Isamu
I
Nakamura
Yukio
Y
D000650:Amnion / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007621:Karyotyping; D039902:Multipotent Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D011247:Pregnancy