Apoptosis-specific activation markers in on- versus off-pump coronary artery bypass graft (CABG) patients.
BACKGROUND
The relation of epithelial/endothelial apoptosis and secretion of death-inducing receptors (DIR) in comparison to vascular adhesion molecules is not known in patients undergoing the On- versus Off-pump coronary artery bypass graft (CABG) procedure.


METHODS
30 patients were prospectively included in the study (On- vs. Off-pump CABG, each n = 15). Serum samples were obtained prior to, and 30 minutes, 60 minutes and 24 hours after CABG operation. ELISA was utilized to detect caspase-cleaved cytokeratin-18 (CK18) by means of M30 antibody, soluble VCAM-1, soluble ICAM-1, and soluble DIR TNFR-1 and CD95.


RESULTS
Soluble caspase-cleaved CK18 was increased and leveled to initial values at 24 hrs. sICAM-1 showed a significant decrease at 30 minutes and 60 minutes in comparison to preoperative values. sTNFR-1/sCD95 showed a rise that was not significant to preoperative values.


CONCLUSIONS
These results indicate for the first time that epithelial/endothelial apoptosis is occurring in patients undergoing bypass operation, irrespective of the CABG procedure selected.
Szerafin
Tamás
T
Department of Cardiac Surgery, University of Debrecen, Debrecen, Hungary.
Horvath
Ambrus
A
Moser
Bernhard
B
Hacker
Stefan
S
Hoetzenecker
Konrad
K
Steinlechner
Barbara
B
Brunner
Markus
M
Roth
Georg
G
Boltz-Nitulescu
George
G
Peterffy
Arpat
A
Wolner
Ernst
E
Ankersmit
Hendrik Jan
HJ
D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D002315:Cardiopulmonary Bypass / Q000009:adverse effects; D001026:Coronary Artery Bypass / Q000009:adverse effects / Q000379:methods*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D007633:Keratins / Q000097:blood; D011446:Prospective Studies; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000097:blood; D012995:Solubility; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood; D019014:fas Receptor / Q000097:blood
D015415:Biomarkers; D047888:Receptors, Tumor Necrosis Factor, Type I; D019010:Vascular Cell Adhesion Molecule-1; D019014:fas Receptor; D018799:Intercellular Adhesion Molecule-1; D007633:Keratins