Gene expression profiles of some immune relevant genes from skin of susceptible and responding Atlantic salmon (Salmo salar L.) infected with Gyrodactylus salaris (Monogenea) revealed by suppressive subtractive hybridisation.
Suppressive subtractive hybridisation was used to examine the genetic basis of susceptibility and resistance of the Atlantic salmon (Salmo salar) to Gyrodactylus salaris infection. Selected immune relevant genes are listed and two genes, for myeloid leukemia differentiation protein (Mcl-1) and opioid growth factor receptor (OGFr), obtained from the susceptible salmon library were characterised. Both sequences showed high amino acid identity and similarity with human and mouse isoforms, and their possible involvement in the response of salmon to G. salaris is discussed. Quantitative reverse transcriptase-PCR was performed for both genes. Upregulation of Mcl-1 in B1 backcross salmon of the susceptible phenotypic category compared with resistant salmon was demonstrated. The possible relationship of the salmon Mcl-1 and cytokines (interleukin 1beta) in the G. salaris-induced host response is discussed. Potential involvement of OGFr in the depletion of mucous cells during prolonged and heavy G. salaris infection, via suppression of DNA synthesis and profound decrease in basal cell proliferation, is proposed. However, only two of six susceptible fish showed high upregulation of OGFr, which might indicate that its expression is localised to sites of wounds resulting from a heavy burden of G. salaris.
Matejusová
Iveta
I
FRS Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB11 9DB, UK. i.matejusova@marlab.ac.uk
Felix
Benjamin
B
Sorsa-Leslie
Tarja
T
Gilbey
John
J
Noble
Leslie R
LR
Jones
Catherine S
CS
Cunningham
Carey O
CO
D000818:Animals; D001483:Base Sequence; D004478:Ectoparasitic Infestations / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology* / Q000469:parasitology; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D006373:Helminthiasis / Q000276:immunology*; D006790:Host-Parasite Interactions; D008969:Molecular Sequence Data; D009093:Mucus / Q000276:immunology; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D011957:Receptors, Opioid / Q000235:genetics; D020317:Salmo salar / Q000235:genetics* / Q000276:immunology; D012867:Skin / Q000276:immunology / Q000293:injuries / Q000469:parasitology*; D015854:Up-Regulation
C579094:Mcl1 protein, mouse; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011957:Receptors, Opioid; C033593:methionine-enkephalin receptor