Voltammetric determination of gamma radiation-induced DNA damage.
Homopolydeoxyribonucleotides, poly[dGuo], poly[dAdo], poly[dThd], and poly[dCyd], and calf thymus single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) aqueous solutions previously exposed to gamma radiation doses between 2 and 35 Gy, were studied by differential pulse voltammetry using a glassy carbon electrode. The interpretation of the voltammetric data was also supported by the electrophoretic migration profile obtained for the same ssDNA and dsDNA gamma-irradiated samples by nondenaturing agarose gel electrophoresis. The generation of 8-oxo-7,8-dihydroguanine, 2,8-dihydroxyadenine, 5-formyluracil, base-free sites, and single- and double-stranded breaks in the gamma-irradiated DNA samples was detected voltammetrically, with the amount depending on the irradiation time. It was found that the current peaks obtained for 8-oxoguanine increase linearly with the radiation dose applied to the nucleic acid sample, and values between 8 and 446 8-oxo-7,8-dihydroguanine (8-oxoGua) per 10(6) guanines per Gy were obtained according to the nucleic acid sample. The results showed that voltammetry can be used for monitoring and simultaneously characterizing different kinds of DNA damage caused by gamma radiation exposure.
Piedade
J A P
JA
Departamento de Química, Universidade de Coimbra, 3004-535 Coimbra, Portugal.
Oliveira
P S C
PS
Lopes
M C
MC
Oliveira-Brett
A M
AM
D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects*; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000528:radiation effects; D003853:Deoxyribonucleosides / Q000737:chemistry / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004563:Electrochemistry / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005720:Gamma Rays; D009841:Oligonucleotides / Q000737:chemistry / Q000528:radiation effects
D004277:DNA, Single-Stranded; D003853:Deoxyribonucleosides; D009841:Oligonucleotides; D004247:DNA