Analysis of L-myc gene polymorphism in patients with renal failure outcome to renal transplant.
BACKGROUND
Abnormalities of cell numbers and apoptosis have been observed in renal failure. As uncontrolled expression of c-myc is known to induce apoptosis, we thought that polymorphism in the other myc gene, L-myc gene, which is structually similar to c-myc and reported to be expressed in the kidney, may have a role in the induction of apoptosis and thus have role in chronic renal failure. The aim of this study was to investigate the relationship between the distribution of L-myc genotypes and renal failure.


METHODS
In the present study we examined 101 chronic renal failure patients who had either live or cadaveric renal transplants and 105 healthy individuals, for L-myc gene polymorphism by polymerase chain reactions and restriction fragment length polymorphism techniques.


RESULTS
Among our patient group, the distribution of the LL, LS, and SS genotypes was 24% (n=25), 71% (n=71), and 5% (n=5), respectively, versus 41% (n=43), 47% (n=49), and 12% (n=13) in our control group. The distribution of genotypes was significantly different between our patients and the control group (chi2=12.281; P=.002). The frequency of the S allele was significantly higher in the patient group (chi2=6.122; P=.013).


CONCLUSIONS
Our study showed that having an S allele in the L-myc gene may increase the risk of renal failure.
Yigit
B
B
Haydarpasa Numune Research and Educational Hospital, Renal Transplantation Unit, Department of Molecular Medicine, Istanbul University, Turkey.
Bozkurt
N
N
Yaylim
I
I
Titiz
I
I
Isbir
T
T
D016259:Genes, myc; D005838:Genotype; D006801:Humans; D007676:Kidney Failure, Chronic / Q000453:epidemiology / Q000235:genetics* / Q000601:surgery*; D016030:Kidney Transplantation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012016:Reference Values; D015183:Restriction Mapping; D012307:Risk Factors; D014019:Tissue Donors / Q000706:statistics & numerical data; D016896:Treatment Outcome