title: Ovarian stimulation with GnRH agonist, but not GnRH antagonist, partially restores the expression of endometrial integrin beta3 and leukaemia-inhibitory factor and improves uterine receptivity in mice.
abstract: BACKGROUND
  The impact of different ovarian stimulation (OS) protocols on endometrial receptivity remains controversial. In this study, the effects of different OS on the expression of endometrial integrin beta3 subunit and leukaemia-inhibitory factor (LIF) during the implantation window and the implantation rate in mice were investigated.
  
  
  METHODS
  Three OS protocols were used, involving either pregnant mare's serum gonadotrophin (PMSG) alone, PMSG plus GnRH agonist or PMSG plus GnRH antagonist. Uterus samples were collected at 48 h after OS or ovulation and were detected with immunohistochemistry, Western blot and RT-PCR analyses. Normal embryos at gestation day 4 were transferred into the uteri of mice in the control and OS groups.
  
  
  RESULTS
  All OS groups showed a significant decrease in the expression of both the endometrial integrin beta3 subunit and LIF during the implantation window and the implantation rate. Among the three OS groups, GnRH agonist-treated mice showed a higher endometrial integrin beta3 subunit and LIF expression and a higher implantation rate. No significant difference was found in the measured indices between the GnRH antagonist and PMSG groups.
  
  
  CONCLUSIONS
  OS may inhibit the expression of endometrial integrin beta3 subunit and LIF and impair endometrial receptivity in mice. OS with GnRH agonist, but not GnRH antagonist, may partially restore the endometrial physiological secretion and improve uterine receptivity.
authors.affiliation: Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Heng-Chao
authors.initials: HC
authors.lastname: Ruan
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Zhu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Luo
authors.forename: Ai-Xia
authors.initials: AX
authors.lastname: Liu
authors.forename: Yu-Li
authors.initials: YL
authors.lastname: Qian
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Zhou
authors.forename: Fan
authors.initials: F
authors.lastname: Jin
authors.forename: He-Feng
authors.initials: HF
authors.lastname: Huang
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Sheng
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D004622:Embryo, Mammalian / Q000502:physiology; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists* / Q000037:antagonists & inhibitors*; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D007150:Immunohistochemistry; D039661:Integrin beta3 / Q000187:drug effects / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D053605:Leukemia Inhibitory Factor; D051379:Mice; D008815:Mice, Inbred Strains; D009028:Morula / Q000187:drug effects / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017329:Triptorelin Pamoate / Q000494:pharmacology*; D014599:Uterus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D006064:Gonadotropins, Equine; D039661:Integrin beta3; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone