title: Direct grafting of RGD-motif-containing peptide on the surface of polycaprolactone films.
abstract: Direct surface modification of biodegradable polycaprolactone (PCL) was performed without the necessity of synthesis of functionisable co-polymers. An easy-to-perform three-step procedure consisting of amination, reaction with hetero-bifunctional cross-linkers and conjugation of an RGD-motif-containing peptide was used to modify polymer films and improve the attachment of endothelial cells. The biological activity of modified surfaces was assessed by estimating microvascular endothelial cell attachment. Covalent coating with RGD resulted in an approximately 11-fold increase of endothelial cell attachment on modified PCL surfaces compared with untreated polymer. The specificity of the attachment enhancement was confirmed by using a control peptide. It is concluded that chemical surface modification is an appropriate method of rendering degradable polymers, such as PCL, cell-adhesive.
authors.affiliation: Department of Orthodontics, Academic Centre for Dentistry Amsterdam (ACTA), Louwesweg 1, 1066 EA Amsterdam, The Netherlands. mgabriel@acta.nl
authors.forename: Matthias
authors.initials: M
authors.lastname: Gabriel
authors.forename: Geerten P
authors.initials: GP
authors.lastname: van Nieuw Amerongen
authors.forename: Victor W M
authors.initials: VW
authors.lastname: Van Hinsbergh
authors.forename: Arie V Van Nieuw
authors.initials: AV
authors.lastname: Amerongen
authors.forename: Andrej
authors.initials: A
authors.lastname: Zentner
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008297:Male; D008422:Materials Testing; D008858:Microscopy, Phase-Contrast; D008956:Models, Chemical; D009842:Oligopeptides / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D009842:Oligopeptides; D010446:Peptide Fragments; D011091:Polyesters; C016240:polycaprolactone; C047981:arginyl-glycyl-aspartic acid