Enzymatic modifications of cephalosporins by cephalosporin acylase and other enzymes.
Semisynthetic cephalosporins are important antibacterials in clinical practice. Semisynthetic cephalosporins are manufactured by derivatizing 7-aminocephalosporanic acid (7-ACA) and its desacetylated form. Microbial enzymes such as D-amino acid oxidase, glutaryl-7-ACA acylase and cephalosporin esterase are being used as biocatalysts for the conversion of cephalosporin C (CEPH-C) to 7-ACA and its desacetylated derivatives. Recent developments in the field of enzymatic modifications of cephalosporin with special emphasis on group of enzymes called as cephalosporin acylase is discussed in this review. Aspects related to screening methods, isolation and purification, immobilization, molecular cloning, gene structure and expression and protein engineering of cephalosporin acylases have been covered. Topics pertaining to enzymatic modifications of cephalosporin by D-amino acid oxidase, cephalosporin methoxylase and beta-lactamase are also covered.
Sonawane
Vijay Chintaman
VC
Institute of Microbial Technology, Sector 39-A, Chandigarh, PIN 160 036, India. vijay@imtech.res.in
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology; D002511:Cephalosporins / Q000819:agonists / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D010399:Penicillin Amidase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D010399:Penicillin Amidase