Effects of surface topography on the connective tissue attachment to subcutaneous implants.
OBJECTIVE
A major concern for implants that penetrate stratified epithelia is aggressive epithelial proliferation and migration. This epithelial downgrowth on the implant can be inhibited by a firm attachment between the underlying connective tissue and the implant. This study evaluates the connective tissue attachment to titanium implants with various well-defined surface topographies.


METHODS
Titanium-coated epoxy replicas of polished (PO; R(a) = 0.06 microm), finely blasted (FB; R(a) = 1.36 microm), coarsely blasted (CB; R(a) = 5.09 microm), acid-etched (AE; R(a) = 0.59 microm), coarsely blasted and acid-etched (SLA; R(a) = 4.39 microm), titanium plasma-sprayed (TPS; R(a) = 5.85 microm), machined-like (ML; R(a) = 2.15 microm), and micromachined grooved (GR; V-shaped grooves 30 microm deep) surfaces were implanted subcutaneously in 74 rats for 1 to 11 weeks. Animals were sacrificed weekly. Surfaces were processed for histomorphometric evaluation of connective tissue attachment, capsule thickness, and where applicable, the degree of separation between the tissue and implant.


RESULTS
A total of 153 test surfaces were analyzed. Statistical analysis revealed that textured and rough substrata, namely the GR, TPS, AE, CB, and SLA surfaces, exhibited significantly greater (P < .05) connective tissue attachment and thinner fibrous encapsulation when compared to the PO surface. Tissue separation from the implant interface was of significantly lower magnitude and frequency with the rough surfaces than with the PO surface.


CONCLUSIONS
The results indicate that rough implant surfaces are associated with stable connective tissue attachment, which has implications for their use in percutaneous and permucosal applications. In addition, data from the AE surface may indicate that the geometry of the surface irregularities can also be a significant determinant of the connective tissue response.
Kim
Hugh
H
Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, Canada.
Murakami
Hiroshi
H
Chehroudi
Babak
B
Textor
Marcus
M
Brunette
Donald M
DM
D000818:Animals; D003238:Connective Tissue / Q000502:physiology*; D003757:Dental Implantation / Q000379:methods*; D015921:Dental Implants; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley
D015921:Dental Implants