Differentiation of bovine Staphylococcus aureus isolates by use of polymorphic tandem repeat typing.
Staphylococcus aureus is a common cause of bovine mastitis. A simple and efficient typing method would be helpful in understanding S. aureus sources and spread. Ninety-six S. aureus strains, isolated between 1961 and 2003 from the milk of 90 dairy cows belonging to 75 French herds, were subjected to multiple-locus variable-number tandem repeats analysis (MLVA) by PCR. The conjunction of clfA, clfB, SAV1078 and fnb gene tandem repeats (TRs) enabled the definition of 61 types. When coa, spa, sdrC, sdrD and sspA TRs were used individually as additional markers, 63, 68, 67, 65 and 67 types were defined, respectively, versus 77 types when they were all included in the method. These additional TRs did not improve the differentiation of isolates collected in the same farm. The MLVA procedure using the tandem repeats embedded in clfA, clfB, SAV1078 and fnb loci as a basic combination at the herd level or associated with other TRs such as spa, sdrC, sdrD, sspA and coa can be a valuable tool for bovine S. aureus epidemiological studies.
Gilbert
Florence B
FB
Unité d'Infectiologie Animale et Santé Publique, Bâtiment 311, Institut National de la Recherche Agronomique, Nouzilly, France. gilbert@tours.inra.fr
Fromageau
Angélina
A
Gélineau
Ludovic
L
Poutrel
Bernard
B
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005260:Female; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020080:Tandem Repeat Sequences