Synthesis of cell-permeable peptide nucleic acids and characterization of their hybridization and uptake properties.
Guanidine-based peptide nucleic acid (GPNA) monomers and oligomers containing all four natural (adenine (A), cytosine (C), guanine (G), and thymine (T)) and two unnatural (2-thiouracil (sU) and 2,6-diaminopurine (D)) nucleobases have been synthesized. Thermal denaturation study showed that GPNA oligomers containing alternate D-backbone configuration bind sequence-specifically to DNA and, when incubated with mammalian cells, localized specifically to the endoplasmic reticulum (ER).
Zhou
Peng
P
Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.
Dragulescu-Andrasi
Anca
A
Bhattacharya
Birendra
B
O'Keefe
Heather
H
Vatta
Paolo
P
Hyldig-Nielsen
Jens J
JJ
Ly
Danith H
DH
D002462:Cell Membrane / Q000187:drug effects*; D002463:Cell Membrane Permeability; D002851:Chromatography, High Pressure Liquid; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D044366:Transition Temperature
D020135:Peptide Nucleic Acids