Enhancement of polyunsaturated fatty acid production by Tn5 transposon in Shewanella baltica.
Transposon Tn5 mutagenesis was used to generate random mutations in Shewanella baltica MAC1, a polyunsaturated fatty acid (PUFA)-producing bacterium. Three mutants produced 3-5 times more eicosapentaenoic acid (EPA 20:5 n-3) compared to the wild type at 10 degrees C. One of the mutants produced 0.3 mg EPA g(-1) when grown at high temperature (30 degrees C). Moreover, 2 mg docosahexaenoic acid (DHA 22:6 n-3) g(-1) was produced by S. baltica mutants at 4 degrees C. Sequencing of insertion mutation(s) showed 96% homology to trimethylamine N-oxide (TMAO) reductase gene and 85% homology to rRNA operons of E. coli. Tn5 transposon mutagenesis therefore is a suitable technique to increase PUFA formation in bacteria.
Amiri-Jami
Mitra
M
Department of Food Science, University of Guelph, N1G 2W1, Guelph, ON, Canada.
Wang
Haifeng
H
Kakuda
Yukio
Y
Griffiths
Mansel W
MW
D004251:DNA Transposable Elements / Q000235:genetics* / Q000502:physiology; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000235:genetics / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D016254:Mutagenesis, Insertional / Q000379:methods*; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics; D020592:Shewanella / Q000235:genetics / Q000378:metabolism*
D004251:DNA Transposable Elements; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D010089:Oxidoreductases, N-Demethylating; C023186:trimethylamine dehydrogenase