Induction of a leaf specific geranylgeranyl pyrophosphate synthase and emission of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in tomato are dependent on both jasmonic acid and salicylic acid signaling pathways.
Two cDNAs encoding geranylgeranyl pyrophosphate (GGPP) synthases from tomato (Lycopersicon esculentum) have been cloned and functionally expressed in Escherichia coli. LeGGPS1 was predominantly expressed in leaf tissue and LeGGPS2 in ripening fruit and flower tissue. LeGGPS1 expression was induced in leaves by spider mite (Tetranychus urticae)-feeding and mechanical wounding in wild type tomato but not in the jasmonic acid (JA)-response mutant def-1 and the salicylic acid (SA)-deficient transgenic NahG line. Furthermore, LeGGPS1 expression could be induced in leaves of wild type tomato plants by JA- or methyl salicylate (MeSA)-treatment. In contrast, expression of LeGGPS2 was not induced in leaves by spider mite-feeding, wounding, JA- or MeSA-treatment. We show that emission of the GGPP-derived volatile terpenoid (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT) correlates with expression of LeGGPS1. An exception was MeSA-treatment, which resulted in induction of LeGGPS1 but not in emission of TMTT. We show that there is an additional layer of regulation, because geranyllinalool synthase, catalyzing the first dedicated step in TMTT biosynthesis, was induced by JA but not by MeSA.
Ament
Kai
K
Swammerdam Institute for Life Sciences, Department of Plant Physiology, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
Van Schie
Chris C
CC
Bouwmeester
Harro J
HJ
Haring
Michel A
MA
Schuurink
Robert C
RC
D000080462:Acyclic Monoterpenes; D000475:Alkenes / Q000378:metabolism*; D000818:Animals; D003517:Cyclopentanes / Q000378:metabolism*; D004926:Escherichia coli; D015870:Gene Expression; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase / Q000235:genetics / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D039821:Monoterpenes / Q000378:metabolism; D054883:Oxylipins; D020156:Salicylic Acid / Q000378:metabolism*; D015398:Signal Transduction; D040102:Tetranychidae / Q000502:physiology; D014169:Transformation, Bacterial
C500824:4,8,12-trimethyl-1,3,7,11-tridecatetraene; D000080462:Acyclic Monoterpenes; D000475:Alkenes; D003517:Cyclopentanes; D039821:Monoterpenes; D054883:Oxylipins; C011006:jasmonic acid; C018584:linalool; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D020156:Salicylic Acid