Effects of alveolar macrophage depletion on liposomal vaccine protection against respiratory syncytial virus (RSV).
Little is known about the identities and roles of antigen-presenting cells upon exposure to antigens of respiratory syncytial virus (RSV). Here, we focused on elucidating the importance of alveolar macrophages in conferring protective immunity in mice administered a liposome-encapsulated recombinant fragment of the RSV G protein. Mice were depleted of alveolar macrophages by intranasal inoculation of liposome-encapsulated dichloromethylenediphosphonic acid (DMDP). Mice depleted of alveolar macrophages prior to immunization developed reduced levels of serum RSV-neutralizing antibody and showed dramatically impaired protection against RSV challenge. The severity of interstitial inflammation was also markedly reduced in macrophage-depleted mice. In conclusion, this study demonstrates a pivotal role for alveolar macrophages during exposure to liposome-encapsulated RSV antigen in initiating both protective and histopathological responses against RSV.
Benoit
A
A
Departments of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.
Huang
Y
Y
Proctor
J
J
Rowden
G
G
Anderson
R
R
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004002:Clodronic Acid / Q000494:pharmacology; D003713:Dendritic Cells / Q000276:immunology; D008081:Liposomes; D008168:Lung / Q000473:pathology / Q000821:virology; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000473:pathology; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage*; D012136:Respiratory Syncytial Viruses; D013879:Thioredoxins / Q000235:genetics; D014611:Vaccination
D008081:Liposomes; D011993:Recombinant Fusion Proteins; D022261:Respiratory Syncytial Virus Vaccines; D004002:Clodronic Acid; D013879:Thioredoxins