31P NMR study on the autophosphorylation of insulin receptors in the plasma membrane.
A nonradioactive 31P nuclear magnetic resonance (NMR) spectroscopy protocol has been developed and used to investigate in vitro autophosphorylation of insulin receptors. Optimum experimental conditions have been explored, and the effects of Mn2+ and phosphocreatine (PCr) on the determination of the phosphorylation reaction have been assayed. The method was used to monitor the time courses of the phosphorylation reaction in solution. The results from this NMR study were in agreement with observations of insulin receptor phosphorylation made by using Western blotting.
Ge
Yili
Y
Department of Chemistry, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China.
Peng
Hong
H
Huang
Kaixun
K
D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D015394:Molecular Structure; D010759:Phosphorus Isotopes / Q000737:chemistry; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D011972:Receptor, Insulin / Q000737:chemistry* / Q000378:metabolism*
D007328:Insulin; D010759:Phosphorus Isotopes; D000255:Adenosine Triphosphate; D011972:Receptor, Insulin