Capillary electrophoresis-mass spectrometry of citrus endophytic bacteria siderophores.
CE-ESI-MS with a liquid sheath interface and IT mass analyzer was used for analysis of siderophores from different strains of Methylobacterium spp. citrus endophyte extracts. Three Methylobacterium strains were investigated according to positive bioassay tests. Bacteria cultures were grown under Fe(III) absence (siderophore producing cultures) and under Fe(III) presence (control cultures). Siderophores were extracted from culture supernatant with polystyrene resins. BGE and sheath-liquid composition were optimized, respectively, in order to assure both, best peak resolution and ESI-MS sensitivity. The best analysis conditions were obtained with 100 mmol/L ammonium bicarbonate at pH 8 as BGE and methanol:H(2)O 25:75 + 0.05% formic acid as sheath liquid. CZE-ESI-MS analysis revealed two possible siderophores, according to bacterium species, presenting M(r) of 1004.3 and 798.3 Da.
Simionato
Ana V Colnaghi
AV
Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, Brazil.
Simó
Carolina
C
Cifuentes
Alejandro
A
Lacava
Paulo Teixeira
PT
Araújo
Wellington Luiz
WL
Azevedo
João Lúcio
JL
Carrilho
Emanuel
E
D001639:Bicarbonates / Q000378:metabolism; D002957:Citrus / Q000382:microbiology*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005561:Formates / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D020580:Methylobacterium / Q000254:growth & development / Q000378:metabolism*; D017262:Siderophores / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014867:Water / Q000737:chemistry
D001639:Bicarbonates; D005561:Formates; D017262:Siderophores; D014867:Water; C030544:formic acid; C027043:ammonium bicarbonate; D007501:Iron; D000432:Methanol