Perphenylcarbamoylated beta-cyclodextrin bonded-silica particles as chiral stationary phase for enantioseparation by pressure-assisted capillary electrochromatography.
Perphenylcarbamoylated beta-cyclodextrin bonded-silica particles (5 microm) were packed into 75-mum fused-silica capillaries, and used for the enantiomer separation of neutral and basic solutes by pressure-assisted capillary electrochromatography. Triethylammonium acetate and phosphate buffer were employed as the BGEs. A cathodic EOF was observed with these two BGEs. Seven chiral analytes were successfully resolved into their enantiomers under optimized conditions, and five of them could be baseline-separated within 12 min due to their high electrophoretic mobility. Better results were achieved with phosphate buffer as the BGE. The effects of organic content and pH on the enantioseparation were also investigated.
Lin
Bo
B
Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China.
Shi
Zhi-Guo
ZG
Zhang
Hui-Juan
HJ
Ng
Siu-Choon
SC
Feng
Yu-Qi
YQ
D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011312:Pressure; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
D002021:Buffers; D047392:beta-Cyclodextrins; C031215:betadex