title: Selectivity in reversed-phase separations Influence of the stationary phase.
abstract: The selectivity difference between 15 different stationary phases was measured using a large number of analytes at 2 or 3 different pH values (3, 7 and 10) with acetonitrile and methanol as the mobile phase modifiers. The packings discussed include standard C(8) and C(18) packings, packings with embedded polar groups, a phenyl packing, a pentafluoro-phenyl packing, an adamantylethyl packing and others. The major selectivity differences observed are discussed in detail. Specific effects such as pi-pi interactions on phenyl packings or hydrogen-bond interactions on phases with embedded polar groups are confirmed.
authors.affiliation: Waters Corporation, 34 Maple Street, Milford, MA 01757, USA. Uwe_Neue@Waters.com
authors.forename: Uwe D
authors.initials: UD
authors.lastname: Neue
authors.forename: John E
authors.initials: JE
authors.lastname: O'Gara
authors.forename: Alberto
authors.initials: A
authors.lastname: Méndez
mesh_terms: D000097:Acetonitriles; D000143:Acids / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006838:Hydrocarbons / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D000432:Methanol; D010636:Phenols / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide; D013449:Sulfonamides / Q000302:isolation & purification
chemical_list: D000097:Acetonitriles; D000143:Acids; D006838:Hydrocarbons; D010636:Phenols; D013449:Sulfonamides; D012822:Silicon Dioxide; D000432:Methanol; C032159:acetonitrile