title: Investigations on the involvement of the lectin pathway of complement activation in anaphylaxis.
abstract: BACKGROUND
  Systemic anaphylaxis is the most severe form of immediate hypersensitivity reaction. The activation of the complement system occurs during anaphylactic shock. The purpose of this study was to determine in a mouse model whether the lectin pathway of complement activation is involved in anaphylaxis.
  
  
  METHODS
  To see whether the lectin pathway is involved in anaphylactic shock, serum mannan-binding lectin (MBL) levels were measured after passive anaphylaxis. Also MBL expression and binding to potential ligands were investigated. To determine whether complement or mast cell activation is essential for hypothermia in anaphylactic shock, mouse strains deficient in MBL-A and MBL-C, C1q, factors B and C2, C5, C5aR, or mast cells were tested.
  
  
  RESULTS
  After antigenic challenge a marked drop in body temperature as well as a rapid decrease in serum MBL levels were observed. The decrease of serum MBL levels in shock could not be attributed to MBL binding to immune complexes or tissues, but an interaction of MBL with mast cell-derived proteoglycans was seen. In contrast to mast cell-deficient mice, none of the complement-deficient mouse strains were protected from shock-associated hypothermia.
  
  
  CONCLUSIONS
  These results indicate that neither MBL nor activation of the complement cascade is crucial for the induction of anaphylaxis. In contrast mast cell activation is associated with the development of hypothermia and possibly the observed decrease in serum MBL levels.
authors.affiliation: Institute of Immunology, University of Regensburg, Regensburg, Germany, and Department of Pediatrics, Laboratory of Developmental Immunology, Massachusetts General Hospital, Boston, USA.
authors.forename: Michaela
authors.initials: M
authors.lastname: Windbichler
authors.forename: Bernd
authors.initials: B
authors.lastname: Echtenacher
authors.forename: Kazue
authors.initials: K
authors.lastname: Takahashi
authors.forename: R Alan B
authors.initials: RA
authors.lastname: Ezekowitz
authors.forename: Wilhelm J
authors.initials: WJ
authors.lastname: Schwaeble
authors.forename: Jens C
authors.initials: JC
authors.lastname: Jenseniuis
authors.forename: Daniela N
authors.initials: DN
authors.lastname: Männel
mesh_terms: D000707:Anaphylaxis / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D000818:Animals; D003167:Complement Activation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007035:Hypothermia / Q000209:etiology / Q000503:physiopathology; D007150:Immunohistochemistry; D037601:Mannose-Binding Lectin / Q000097:blood / Q000378:metabolism*; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D008817:Mice, Mutant Strains; D010047:Ovalbumin / Q000276:immunology; D016133:Polymerase Chain Reaction; D011509:Proteoglycans / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D037601:Mannose-Binding Lectin; D011509:Proteoglycans; D012333:RNA, Messenger; D010047:Ovalbumin