title: Dehydroepiandrosterone and human adipose tissue.
abstract: OBJECTIVE
  The aim of this study was to investigate whether DHEA alters the proliferation and differentiation of human sc and visceral adipose cells in primary cultures.
  
  
  METHODS
  Sc and omental adipose tissue was obtained from 10 female donors aged 36+/-3.6 yr with a body mass index (BMI) of 33+/-3.21 kg/m2. Stromal vascular cells were isolated and cultured using modified procedures described by Entenmann and Hauner. For the proliferation assay, stromal-vascular cells from sc and visceral adipose tissue cultures were fed with proliferation media containing 0, 25 or 100 microM DHEA for 3 days. At the end of this treatment period, two type cultures were prepared for determining their metabolic activity using the sulforhodamine B staining procedure.
  
  
  RESULTS
  The metabolic activity of proliferating human visceral adipose tissue was higher than sc adipose tissue. The activity of proliferating human visceral tissue cultures decreased more than the sc tissue as the level of DHEA in the cultures was increased.
  
  
  CONCLUSIONS
  These data suggest that DHEA predominantly influences the proliferation and differentiation of human omental adipose tissue.
authors.affiliation: Department of Endocrinology and Metabolism, Ege University Hospital, 35100 Izmir, Turkey. fuldensarac@yahoo.com
authors.forename: F
authors.initials: F
authors.lastname: Saraç
authors.forename: S
authors.initials: S
authors.lastname: Yildiz
authors.forename: F
authors.initials: F
authors.lastname: Saygili
authors.forename: G
authors.initials: G
authors.lastname: Ozgen
authors.forename: C
authors.initials: C
authors.lastname: Yilmaz
authors.forename: T
authors.initials: T
authors.lastname: Kabalak
authors.forename: M
authors.initials: M
authors.lastname: Tüzün
mesh_terms: D050153:Abdominal Fat / Q000166:cytology; D000273:Adipose Tissue / Q000187:drug effects*; D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009852:Omentum / Q000166:cytology; D050151:Subcutaneous Fat / Q000166:cytology
chemical_list: D003687:Dehydroepiandrosterone