Molecular mechanisms associated with donor-specific microchimerism in peripheral blood of Brazilian patients after liver transplantation.
A large number of studies in liver transplantation have demonstrated allogeneic microchimerism. The clinical and immunologic implications of this finding remain inconclusive, just as the influence of HLA mismatch and donor alloreactivity also are controversial. The present study analyzed the presence of allogeneic microchimerism in liver transplant recipients in relation to donor leukocyte kinetics and rejection episodes. The study was extended to determining the influence of immunogenetic factors in patients after liver transplantation. The presence of allogeneic microchimerism was analyzed on peripheral blood of 50 recipients. DNA extracted from the samples was subjected to typing for HLA-DRB1 and -DQB1 alleles by polymerase chain reactions using sequence-specific primers (PCR/SSP). Microchimerism was identified by nested PCR/SSP. Microchimerism was detected in 72% of patients. There was significant effect of microchimerism on rejection episodes (P=.002), while HLA mismatches did not show significance for one or two mismatches (P=.98). Allogeneic microchimerism detected in the majority of liver transplant patients was observed to be significantly associated with rejection episodes.
Araújo
M B
MB
Immunogenetics Transplant Laboratory, Clinical Pathology Department, School of Medical Science, Campinas, Brazil.
Leonardi
L S
LS
Boin
I F S F
IF
Leonardi
M I
MI
Meirelles
L
L
Magna
L A
LA
Donadi
E A
EA
Kraemer
M H S
MH
D001938:Brazil; D004247:DNA / Q000097:blood / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing; D006801:Humans; D016031:Liver Transplantation / Q000276:immunology / Q000502:physiology*; D018183:Transplantation Chimera; D014184:Transplantation, Homologous
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D004247:DNA