Structural features in carrageenan that interact with a heparin-binding hematopoietic growth factor and modulate its biological activity.
The effects of carrageenans' structural features on its interaction with granulocyte colony-stimulating factor (G-CSF) and on the growth and differentiation of a G-CSF dependent leukemia cell line (NFS-60) were studied. lambda, iota, and kappa carrageenans, with decreasing contents of sulfation, bound to G-CSF with binding constants of (6.2+/-0.6) x 10(5)M(-1), (7.4+/-0.5) x 10(5)M(-1) and (6.0+/-0.4) x 10(5)M(-1), and with 27.7+/-0.2, 17.4+/-0.1 and 8.4+/-0.1 binding sites, respectively. However, kappa carrageenan oligosaccharide had no affinity for G-CSF. The three carrageenans significantly inhibited G-CSF-induced growth of NFS-60 cells. The high sulfate content lambda carrageenan could also induce the maturation of the cells, but relatively low sulfate content iota and kappa carrageenans could not. The results suggested that G-CSF-carrageenan bindings were dependent on carrageenans' sulfate contents and chain lengths, which could also affect the growth and differentiation of NFS-60 cells.
Liang
Aiye
A
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Zhou
Xiaomian
X
Wang
Qiuyan
Q
Liu
Xin
X
Liu
Xiaojun
X
Du
Yuguang
Y
Wang
Keyi
K
Lin
Bingcheng
B
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002351:Carrageenan / Q000737:chemistry / Q000378:metabolism*; D002453:Cell Cycle; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019075:Electrophoresis, Capillary; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D006801:Humans
D016179:Granulocyte Colony-Stimulating Factor; D002351:Carrageenan; D006493:Heparin