title: Long telomeres in the mature human placenta.
abstract: OBJECTIVE
  To investigate whether telomere shortening may play a role in senescence of the placenta.
  
  
  METHODS
  Villous tissue was collected from single, random sites of full-term placentas (39-41 weeks of gestation; n=10) as well as multiple, specific sites of the same placenta (39-41 weeks of gestation; n=5). For the latter group of placentas, samples were taken near the umbilical cord and at the periphery on both the maternal and fetal sides (a total of 4 samples per placenta). Cord blood samples were also obtained from all placental donors. Telomerase activity was assessed by the TRAP assay, and telomere length measured by Southern analysis of mean terminal restriction fragment (TRF) length.
  
  
  RESULTS
  We show for the first time that telomeres are longer ( approximately 25% longer; P<0.001) in placenta tissue than in cord blood from the same donor.
  
  
  CONCLUSIONS
  Telomere shortening is unlikely to have a significant role in senescence or terminal maturation of the placenta.
authors.affiliation: Department of Anatomy and Reproductive Biology, Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii, 1960 East-West Road, E124, Honolulu, HI 96822, USA. allsopp@hawaii.edu
authors.forename: R
authors.initials: R
authors.lastname: Allsopp
authors.forename: J
authors.initials: J
authors.lastname: Shimoda
authors.forename: D
authors.initials: D
authors.lastname: Easa
authors.forename: K
authors.initials: K
authors.lastname: Ward
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D002824:Chorionic Villi / Q000201:enzymology / Q000254:growth & development*; D004247:DNA / Q000032:analysis; D005312:Fetal Blood / Q000378:metabolism; D005865:Gestational Age; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000032:analysis; D019098:Telomerase / Q000097:blood / Q000235:genetics; D016615:Telomere / Q000502:physiology*; D013997:Time Factors
chemical_list: D012313:RNA; D004247:DNA; C509186:TERT protein, human; D019098:Telomerase