A periplasmic glutamate/aspartate binding protein from Shigella flexneri: Gene cloning, over-expression, purification and preliminary crystallographic studies of the recombinant protein.
Periplasmic substrate binding proteins (PSBPs) are essential components of the bacterial periplasmic transport system, which transports a wide variety of nutrients from the periplasmic space to the cytoplasm. The glutamate/aspartate binding protein SfGlu/AspBP is a unique PSBP consisting of 279 amino acid residues. The SfGlu/AspBP gene was cloned, over-expressed, and purified by immobilized metal ion affinity chromatography and size-exclusion chromatography. The recombinant protein SfGlu/AspBP has been crystallized by the hanging-drop vapor-diffusion method and its X-ray diffraction data were collected at an atomic resolution of 1.15 A. The crystals belong to the space group P2(1) with unit cell parameters: a = 48.41 A, b = 68.18 A, c = 80.21 A and beta = 98.78 degrees. There are two molecules per asymmetric unit.
Fan
Cheng-Peng
CP
Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101, People's Republic of China.
Zhu
De-Yu
DY
Lu
Hong-Xia
HX
Jin
Qi
Q
Wang
Da-Cheng
DC
D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000378:metabolism; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D018698:Glutamic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D019897:Periplasm; D033902:Periplasmic Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012763:Shigella flexneri / Q000378:metabolism*
D033902:Periplasmic Binding Proteins; D011994:Recombinant Proteins; D001224:Aspartic Acid; D018698:Glutamic Acid