Rapid analysis of coumarins using surface plasmon resonance.
Coumarin molecules are ubiquitous in nature. Several have come to prominence as potential clinical therapeutic candidates. The principal example is warfarin, which is a very widely prescribed anticoagulant. Other coumarin derivatives, such as aflatoxin B1, are insidious contaminants in crop-derived foodstuffs. Extreme potency is a common feature of all biochemically active coumarins and, thus reliable methods for their rapid and sensitive detection are of paramount importance. Accordingly, this review examines the current methods used in the analysis of these molecules and compares them with immunoassay-based strategies. As a case study, we report on our experiences with using coumarin-specific polyclonal, monoclonal, and recombinant antibodies in conjunction with a surface plasmon resonance-based biosensor for analysis of coumarins. We chart the assay development process and demonstrate high sensitivity and reproducibility that compares favorably with established methodologies.
Lacy
Aoife
A
Dublin City University, National Centre for Sensor Research, School of Biotechnology, Dublin 9, Ireland.
Dunne
Lynsey
L
Fitzpatrick
Brian
B
Daly
Stephen
S
Keating
Gary
G
Baxter
Andrew
A
Hearty
Stephen
S
O'Kennedy
Richard
R
D016604:Aflatoxin B1 / Q000032:analysis; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D008956:Models, Chemical; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance / Q000379:methods*; D014468:Umbelliferones / Q000032:analysis; D014859:Warfarin / Q000037:antagonists & inhibitors / Q000494:pharmacology
D003374:Coumarins; D007074:Immunoglobulin G; D014468:Umbelliferones; D014859:Warfarin; C031477:7-hydroxycoumarin; D016604:Aflatoxin B1; C030123:coumarin