title: The (ADP-ribosyl)ation reaction in thermophilic bacteria.
abstract: Species of Alicyclobacillus, Bacillus and Thermus genera were selected in order to study the possible presence of the (ADP-ribosyl)ation system. These bacteria are thermophilic, aerobic, and were isolated from different geothermal sources. Both activity and expression of (ADP-ribosyl)ating proteins were tested in cells at different growth phases, and evidence of an active system was obtained in all analyzed microorganisms, with comparable enzymatic levels. Immunochemical analyses with polyclonal antibodies against both eukaryotic anti-(ADP-ribose) transferase and anti-poly(ADP-ribose) polymerase revealed, for all tested organisms, an immunosignal localized in the range of molecular masses between 43-53 kD. Several proteins of various molecular masses were found as ADP-ribose acceptors. Reaction product analyses showed mono(ADP-ribose) to be the only synthesized compound.
authors.affiliation: Dipartimento di Biologia Strutturale e Funzionale, Facolta' di Scienze M.F.N., Universita Federico II, Via Cynthia, 80126 Napoli, Italy. faraone@unina.it
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Faraone-Mennella
authors.forename: Anna
authors.initials: A
authors.lastname: De Maio
authors.forename: Anna
authors.initials: A
authors.lastname: Petrella
authors.forename: Maria
authors.initials: M
authors.lastname: Romano
authors.forename: Patrizia
authors.initials: P
authors.lastname: Favaloro
authors.forename: Agata
authors.initials: A
authors.lastname: Gambacorta
authors.forename: Licia
authors.initials: L
authors.lastname: Lama
authors.forename: Barbara
authors.initials: B
authors.lastname: Nicolaus
authors.forename: Benedetta
authors.initials: B
authors.lastname: Farina
mesh_terms: D036002:ADP Ribose Transferases / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology / Q000254:growth & development; D001419:Bacteria / Q000201:enzymology* / Q000254:growth & development; D004789:Enzyme Activation; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D013824:Thermus / Q000201:enzymology / Q000254:growth & development
chemical_list: D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases