Usability of size-excluded fractions of soy protein hydrolysates for growth and viability of Chinese hamster ovary cells in protein-free suspension culture.
To investigate the effect of size-excluded fraction of non-animal protein hydrolysate on growth, viability and longevity of Chinese hamster ovary (CHO) cells, several commercially available protein hydrolysates were evaluated as a feed supplement to chemically-defined protein-free suspension culture. Soy protein hydrolysates showed better supporting capability for cell growth and viability than the other types of hydrolysates. Maximal cell growth was not affected greatly by size exclusion of some soy hydrolysates such as bacto soytone and soy hydrolysates. CHO cells supplemented with size-excluded fractions of the two hydrolysates showed viable cell density and viability almost equal to those with their crude hydrolysates, although soy hydrolysates showed a little better performance. This suggested that the size-excluded hydrolysate fractions of some soy hydrolysate might be a potential culture medium additive to achieve better downstream operation in a large-scale production as well as enhanced productivity.
Chun
Bok-Hwan
BH
College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
Kim
Jong-Hwang
JH
Lee
Ho-Joung
HJ
Chung
Namhyun
N
D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D002452:Cell Count; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D006224:Cricetinae; D003412:Cricetulus; D016895:Culture Media, Serum-Free; D005947:Glucose / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology*; D030262:Soybean Proteins / Q000737:chemistry* / Q000378:metabolism
D016895:Culture Media, Serum-Free; D011492:Protein Hydrolysates; D030262:Soybean Proteins; D005947:Glucose; D007785:Lactose