Phenolic components and antioxidant activity of Fernblock, an aqueous extract of the aerial parts of the fern Polypodium leucotomos.
Fernblock, an aqueous extract of the aerial parts of the fern Polypodium leucotomos, used as raw material for topical and oral photoprotective formulations, was fractioned by HPLC and the main components with antioxidant capability were identified by means of UV spectra, electrochemical detection, and MSn. Phenolic compounds were identified as 3,4-dihydroxybenzoic acid, 4-hydroxybenzoic acid, vanillic acid, caffeic acid, 4-hydroxycinnamic acid, 4-hydroxycinnamoyl-quinic acid, ferulic acid, and five chlorogenic acid isomers. Total ferric antioxidant capacity (FRAP) of HPLC eluted fractions was measured. The results suggest that the herein identified compounds support, at least partially, the antioxidant and radical scavenging capacities of Fernblock.
Garcia
F
F
Industrial Farmaceutica Cantabria, Arequipa 1, Madrid, Spain. fernando.garcia@ifc.es
Pivel
J P
JP
Guerrero
A
A
Brieva
A
A
Martinez-Alcazar
M P
MP
Caamano-Somoza
M
M
Gonzalez
S
S
D000975:Antioxidants / Q000032:analysis / Q000737:chemistry*; D002109:Caffeic Acids / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis / Q000737:chemistry; D004563:Electrochemistry; D005290:Ferric Compounds / Q000737:chemistry; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010226:Parabens / Q000032:analysis / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D032494:Polypodium; D011422:Propionates; D014641:Vanillic Acid / Q000032:analysis / Q000737:chemistry
D000975:Antioxidants; D002109:Caffeic Acids; D003373:Coumaric Acids; D005290:Ferric Compounds; D010226:Parabens; D010636:Phenols; D010936:Plant Extracts; D011422:Propionates; C513114:fernblock; D014641:Vanillic Acid; C495469:p-coumaric acid; C038193:4-hydroxybenzoic acid; C040048:caffeic acid