Genomic instability of human aberrant crypt foci measured by inter-(simple sequence repeat) PCR and array-CGH.
Aberrant crypt foci (ACF) are the earliest identifiable neoplastic lesions in the colon. Thirty-two ACFs were examined for genomic instability in forms detectable either by inter-(simple sequence repeat) PCR or by array comparative genomic hybridization [array-CGH]. One-fourth of ACFs revealed moderate instability by inter-(simple sequence repeat) PCR; none showed amplifications or deletions on array-CGH. The absence of genomic events detectible by BAC array-CGH indicates early events in colorectal tumor progression are typically smaller than the approximate 150 kb size of a BAC clone insert.
Alrawi
Sadir J
SJ
Division of Gastrointestinal Surgery, Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA.
Carroll
Robert E
RE
Hill
Hank C
HC
Gibbs
John F
JF
Tan
Dongfeng
D
Brenner
Bruce M
BM
Nowak
Norma J
NJ
Swede
Helen
H
Stoler
Daniel L
DL
Anderson
Garth R
GR
D000369:Aged, 80 and over; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology*; D005260:Female; D015894:Genome, Human / Q000235:genetics; D042822:Genomic Instability / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology*