title: Identification and gene expression of the bovine C-type lectin Dectin-1.
abstract: C-type lectin receptors (CTLR) are cell-surface signalling molecules that recognize a range of highly conserved pathogen molecules and instigate the appropriate immune response. Here, we report the cloning, sequencing, mapping and expression pattern of the bovine C-type lectin domain family 7, member A (CLEC7A; synonyms CLCSF12, Dectin-1). We identified two isoforms, similar to the human system, with a long and short neck. Overall, the organization of the two bovine CLEC7A genes is similar to that of humans and mice. The CLEC7A gene maps on Bos taurus chromosome 5 (BTA5). mRNA transcripts for CLEC7A were detected in bone-marrow cells, monocytes, macrophages and dendritic cells and NK cells, but not in CD4(+) T-cells or CD21(+) B-cells. The increased knowledge of the genomic organization of the bovine CTLR genes may promote our understanding of their evolution and help in the identification of bovine genes underlying disease-resistance traits.
authors.affiliation: Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield, AL9 7TA, UK.
authors.forename: S
authors.initials: S
authors.lastname: Willcocks
authors.forename: Y
authors.initials: Y
authors.lastname: Yamakawa
authors.forename: A
authors.initials: A
authors.lastname: Stalker
authors.forename: T J
authors.initials: TJ
authors.lastname: Coffey
authors.forename: T
authors.initials: T
authors.lastname: Goldammer
authors.forename: D
authors.initials: D
authors.lastname: Werling
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics / Q000276:immunology*; D002874:Chromosome Mapping / Q000662:veterinary; D003001:Cloning, Molecular; D005260:Female; D037181:Lectins, C-Type; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D020033:Protein Isoforms; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D000099181:Dectin-1
chemical_list: D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D012313:RNA; D000099181:Dectin-1