In vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substitute.
This study aims to investigate the survival and osteogenic behavior of murine-derived adipose-tissue stromal cells (ATSCs) encapsulated in alginate microcapsules thereby instigating further studies in this cell delivery strategy for in vivo osteogenesis. Cell viability was quantified using a tetrazolium-based assay and osteogenic differentiation was evaluated by both alkaline-phosphatase (ALP) histochemistry and osteocalcin mRNA analysis. Following microencapsulation, cell numbers increased from 3.9 x 10(3) on day 1 to 7.8 x 10(3) on day 7 and maintained excellent viability in the course of 21-day culture. ALP was 6.9, 5.5, and 3.2 times higher than monolayer cultures on days 7, 14, and 21, respectively. In addition, osteocalcin mRNA was detectable in encapsulated cultures earlier (day 14) than monolayer cultures. We conclude that alginate microcapsules can act as three-dimensional matrix for ATSC proliferation and has potential for use as injectable, biodegradable scaffold in bone tissue engineering.
Abbah
S A
SA
Department of Orthopaedics and Traumatology, The University of Hong Kong, Pokfulam.
Lu
W W
WW
Chan
D
D
Cheung
K M C
KM
Liu
W G
WG
Zhao
F
F
Li
Z Y
ZY
Leong
J C Y
JC
Luk
K D K
KD
D017667:Adipocytes / Q000166:cytology* / Q000637:transplantation*; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018786:Bone Substitutes / Q000008:administration & dosage* / Q000737:chemistry*; D016025:Bone Transplantation / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007267:Injections / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D010006:Osteoblasts / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation; D023822:Tissue Engineering / Q000379:methods*
D000464:Alginates; D001672:Biocompatible Materials; D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D006603:Hexuronic Acids; D020723:Glucuronic Acid