title: Study of Pax6 mutant rat revealed the association between upper incisor formation and midface formation.
abstract: In this study, we investigated the process of supernumerary upper incisor formation in the Pax6 mutant rat, rSey(2)/rSey(2), which exhibits a facial cleft between the medial nasal and maxillary processes. Histological investigation and epithelial labeling studies of wild type rat embryos indicated that the upper incisor develops by fusion of two primary dental placodes (PDPs) in the medial nasal process with a contribution from the epithelium of the maxillary process. In the rSey(2)/rSey(2) embryo, both PDPs are formed but they stay apart, then subsequently these PDPs independently develop into upper incisor tooth buds. In order to examine if the failure of the two placodes to fuse is due to the cleft between the maxillary and medial nasal processes, maxillary and medial nasal process fusion was inhibited with a barrier in wild type embryos. This resulted in the maintenance of the two distinct PDPs. These results demonstrate that fusion of the facial processes reduces the number of odontogenic placodes and is required to assemble all components at one site for rat upper incisor formation. The results also provide further insight into the mechanism of supernumerary incisor formation in human cleft lip conditions.
authors.affiliation: Section of Molecular Craniofacial Embryology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Rungarun
authors.initials: R
authors.lastname: Kriangkrai
authors.forename: Suconta
authors.initials: S
authors.lastname: Chareonvit
authors.forename: Koichi
authors.initials: K
authors.lastname: Yahagi
authors.forename: Michio
authors.initials: M
authors.lastname: Fujiwara
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Eto
authors.forename: Sachiko
authors.initials: S
authors.lastname: Iseki
mesh_terms: D000818:Animals; D004848:Epithelium / Q000196:embryology; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism*; D005145:Face / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism*; D007180:Incisor / Q000196:embryology* / Q000378:metabolism*; D008437:Maxilla / Q000196:embryology; D009154:Mutation / Q000235:genetics*; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051761:Paired Box Transcription Factors; C000605733:Pax6 protein, rat; D012097:Repressor Proteins