Promoter cleavage: a topoIIbeta and PARP-1 collaboration.
Hormones trigger dramatic changes in the structure and transcriptional activity of specific promoters that lead to exchange of repression complexes for activation complexes. now show that estrogen-dependent restructuring and transcription of the pS2 promoter require the generation of a DNA double-strand break by a novel protein complex containing two enzymes, topoisomerase IIbeta and poly(ADP-ribose) polymerase.
Lis
John T
JT
Department of Molecular Biology & Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA. jtl10@cornell.edu
Kraus
W Lee
WL
D004250:DNA Topoisomerases, Type II / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D006801:Humans; D008954:Models, Biological; D046912:Multiprotein Complexes; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000737:chemistry / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000187:drug effects; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins / Q000235:genetics
D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins; D004958:Estradiol; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D004250:DNA Topoisomerases, Type II