Effects of the Fusarium toxin deoxynivalenol on tissue protein synthesis in pigs.
The Fusarium toxin deoxynivalenol is known to be a potent protein synthesis inhibitor according to research in different in vitro and in vivo systems. However, the significance of this toxic feature was not yet evaluated for the pig. Therefore, an experiment was carried out to measure porcine tissue protein synthesis employing the so-called flooding dose technique using [(2)H(5)]-phenylalanine as tracer. A total of 25 male castrated pigs (initial weight of 23kg) were used. Seventeen pigs were fed the control diet (control) and eight the Fusarium toxin-contaminated diet (chronic oral deoxynivalenol (DON)) for approximately 4wks. Pigs recruited from the control group were treated with acute oral (n=7) and intravenous (i.v.) DON (n=5), and were exposed to the toxin once on the day of protein synthesis measurement. The DON-dose given prior to the beginning of protein synthesis measurement amounted to 2, 77, 83 and 53microg DONkg(-1) live weight for the control group, chronic oral DON-group, acute oral DON-group and acute i.v. DON-group, respectively. Protein synthesis expressed as fractional synthesis rate (FSR) was significantly reduced in kidneys, spleen and ileum of DON-exposed pigs with effects being less pronounced in the acute oral DON-group. FSR of liver, skeletal and heart muscle, mesenteric lymph nodes, duodenum, jejunum, jejunal mucosa cells, pancreas and lung were not affected by DON.
Dänicke
S
S
Institute of Animal Nutrition, Federal Agricultural Research Centre (FAL), Braunschweig, Bundesallee 50, D-38116 Braunschweig, Germany. sven.daenicke@fal.de
Goyarts
T
T
Döll
S
S
Grove
N
N
Spolders
M
M
Flachowsky
G
G
D000418:Albumins / Q000096:biosynthesis*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004063:Digestion / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005340:Fibrinogen / Q000096:biosynthesis*; D005670:Fusarium / Q000378:metabolism*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000139:chemically induced*; D014255:Trichothecenes / Q000378:metabolism / Q000633:toxicity*
D000418:Albumins; D014255:Trichothecenes; D005340:Fibrinogen; C007262:deoxynivalenol