title: Free-radical scavenging by Ouratea parviflora in experimentally-induced liver injuries.
abstract: The antioxidant potential of crude extracts and fractions from leaves of Ouratea parviflora, a Brazilian medicinal plant used for the treatment of inflammatory diseases, was investigated in vitro through the scavenging of radicals 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), hydroxyl radical (HO*), superoxide anion (O2*-), and lipid peroxidation in rat liver homogenate. The crude extract (CEOP) and hydro-alcoholic fraction (OP4) showed strong inhibitory activity toward lipid peroxidation induced by tert-butyl peroxide (IC50 = 2.3 +/- 0.2 and 1.9 +/- 0.1 microg/ml, respectively). The same products exhibited a strong concentration-dependent inhibition of deoxyribose oxidation (14.9 +/- 0.2 and 0.2 +/- 0.1 microg/ml, respectively), and also showed a considerable antioxidant activity against O2*- (87.3 +/- 0.1 and 73.1 +/- 0.4 microg/ml, respectively) and DPPH radicals (55.4 +/- 0.3 and 38.3 +/- 0.4 microg/ml, respectively). The protective effects of CEOP and OP4 were also studied in mouse liver. CCl4 significantly increased (by 90%) levels of lipid hydroperoxides, carbonyl protein content (64%), DNA damage index (133%), aspartate aminotransferase (261%), alanine aminotransferase (212%), catalase activity (23%), and also caused a decrease of 60% in GSH content. The results showed that CEOP and OP4 exerted cytoprotective effects against oxidative injury caused by CCl4 in rat liver, probably related to the antioxidant activity showed by the in vitro free radical scavenging property.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: Karina Azambuja
authors.initials: KA
authors.lastname: Carbonari
authors.forename: Eduardo Antonio
authors.initials: EA
authors.lastname: Ferreira
authors.forename: Jussara Mattos
authors.initials: JM
authors.lastname: Rebello
authors.forename: Karina Bettega
authors.initials: KB
authors.lastname: Felipe
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Rossi
authors.forename: Joana D'arc
authors.initials: JD
authors.lastname: Felício
authors.forename: Danilo Wilhelm
authors.initials: DW
authors.lastname: Filho
authors.forename: Rosendo Augusto
authors.initials: RA
authors.lastname: Yunes
authors.forename: Rozangela Curi
authors.initials: RC
authors.lastname: Pedrosa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000378:metabolism*; D005609:Free Radicals; D008099:Liver / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D043842:Ochnaceae / Q000378:metabolism*; D018384:Oxidative Stress; D010936:Plant Extracts / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013481:Superoxides