title: Disturbance of cerebellar synaptic maturation in mutant mice lacking BSRPs, a novel brain-specific receptor-like protein family.
abstract: By DNA cloning, we have identified the BSRP (brain-specific receptor-like proteins) family of three members in mammalian genomes. BSRPs were predominantly expressed in the soma and dendrites of neurons and localized in the endoplasmic reticulum (ER). Expression levels of BSRPs seemed to fluctuate greatly during postnatal cerebellar maturation. Triple-knockout mice lacking BSRP members exhibited motor discoordination, and Purkinje cells (PCs) were often innervated by multiple climbing fibers with different neuronal origins in the mutant cerebellum. Moreover, the phosphorylation levels of protein kinase Calpha (PKCalpha) were significantly downregulated in the mutant cerebellum. Because cerebellar maturation and plasticity require metabotropic glutamate receptor signaling and resulting PKC activation, BSRPs are likely involved in ER functions supporting PKCalpha activation in PCs.
authors.affiliation: Department of Anatomy, Hokkaido University, School of Medicine, Sapporo, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Kouichi
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Uda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Shin-ya
authors.initials: SY
authors.lastname: Saito
authors.forename: Miyuki
authors.initials: M
authors.lastname: Nishi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kume
authors.forename: Akira
authors.initials: A
authors.lastname: Tohgo
authors.forename: Izumi
authors.initials: I
authors.lastname: Kaneko
authors.forename: Hisatake
authors.initials: H
authors.lastname: Kondo
authors.forename: Kohji
authors.initials: K
authors.lastname: Fukunaga
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kano
authors.forename: Masahiko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takeshima
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development*; D003712:Dendrites / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D004789:Enzyme Activation / Q000235:genetics; D016678:Genome / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008818:Mice, Neurologic Mutants; D005810:Multigene Family / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000378:metabolism*; D010766:Phosphorylation; D051571:Protein Kinase C-alpha / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000235:genetics; D011597:Psychomotor Performance / Q000502:physiology; D011689:Purkinje Cells / Q000166:cytology / Q000502:physiology*; D017470:Receptors, Glutamate / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013569:Synapses / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D017470:Receptors, Glutamate; D051571:Protein Kinase C-alpha