title: Serum proteomic features for detection of endometrial cancer.
abstract: To find new potential biomarkers for detection of endometrial cancer (EC), 70 serum samples including 40 from EC patients and 30 from normal healthy females were detected by surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF-MS) using WCX2 (weak cation exchange) protein chip. Mass spectra were then assessed with three powerful data-mining tools: a tree classifier, Biomarker Wizard software, and Biomarker Patterns System. The diagnostic pattern combined with 13 potential biomarkers could differentiate EC patients from healthy persons, with a specificity of 100%, sensitivity of 92.5%, and total coincidence of 95.7%. The combination of surface-enhanced laser desorption-ionization with bioinformatics tools could help find new biomarkers and establish with high sensitivity and specificity for the detection of EC.
authors.affiliation: Department of Obstetrics and Gynecology, First Hospital of Peking University, Beijing, China.
authors.forename: L-R
authors.initials: LR
authors.lastname: Zhu
authors.forename: W-Y
authors.initials: WY
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.forename: Y-H
authors.initials: YH
authors.lastname: Zheng
authors.forename: J-Z
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Q-P
authors.initials: QP
authors.lastname: Liao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D018269:Carcinoma, Endometrioid / Q000175:diagnosis; D016022:Case-Control Studies; D003663:Decision Trees; D016889:Endometrial Neoplasms / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009363:Neoplasm Proteins / Q000032:analysis*; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins