title: Obesity-related leptin receptor polymorphisms and gallstones disease.
abstract: OBJECTIVE
  Investigate the association between polymorphisms in the leptin receptor gene associated with obesity and gallstone disease.
  
  
  METHODS
  We conducted a cross-sectional study, carried out at a tertiary setting.
  
  
  METHODS
  We enrolled 97 subjects, comprising 54 subjects with gallstones (cases) and 43 controls (without gallstones).
  
  
  METHODS
  Diet was assessed using a validated questionnaire for the Mexican population. Body mass index, waist circumference, serum glucose,insulin, leptin, lipids and lipoproteins levels were measured. Insulin resistance was calculated by HOMA-IR. Genomic DNA was isolated from lymphoblastoid cells, and Q223R and K656N polymorphisms in the leptin receptor gene were typed using polymerase chain reaction. Unconditional univariate logistic regression analysis was conducted to estimate the probability of gallstone disease associated with the polymorphisms as main effect.
  
  
  RESULTS
  Cases were different in gender(40.74% males in cases vs 74.41% in controls; p < 0.001), older (49.74 vs 44.83 years; p < 0.05), and had more body fat (32.34% vs 28.14%; p = 0.01). Individuals carrying the polymorphism Q223R exhibited a higher BMI (28.44 +/- 6.6 kg/m2 vs 25.94 +/- 3.67 kg/m2, p < 0.05) and waist circumference (96.7 +/- 16.39 cm vs 89.2 +/- 11.05 cm, p < 0.05). In univariate analysis, we did not observe a relation between the presence of a R223 or N656 genotype and gallstone disease in our population (OR = 0.78, 95% CI 0.35-1.73).
  
  
  CONCLUSIONS
  Obesity-related leptin receptor polymorphisms are not associated with gallstones disease.
authors.affiliation: Biomedical Research Department & Liver Unit, Medica Sur Clinic & Foundation, Mexico City, Mexico. nmendez@medicasur.org.mx
authors.forename: Nahum
authors.initials: N
authors.lastname: Méndez-Sánchez
authors.forename: Luisa
authors.initials: L
authors.lastname: Bermejo-Martínez
authors.forename: Norberto C
authors.initials: NC
authors.lastname: Chávez-Tapia
authors.forename: Daniel
authors.initials: D
authors.lastname: Zamora-Valdés
authors.forename: Karla
authors.initials: K
authors.lastname: Sánchez-Lara
authors.forename: Martha H
authors.initials: MH
authors.lastname: Uribe-Ramos
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Ponciano-Rodríguez
authors.forename: Hector A
authors.initials: HA
authors.lastname: Baptista-González
authors.forename: Martha H
authors.initials: MH
authors.lastname: Ramos
authors.forename: Misael
authors.initials: M
authors.lastname: Uribe
mesh_terms: D000328:Adult; D005260:Female; D042882:Gallstones / Q000150:complications* / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D016015:Logistic Models; D008297:Male; D008800:Mexico; D008875:Middle Aged; D009765:Obesity / Q000150:complications* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics*; D054411:Receptors, Leptin
chemical_list: C515448:LEPR protein, human; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin