Expression and localization of hepatocyte growth factor and its receptor c-Met in inverted papillomas.
CONCLUSIONS
The results suggest that elevated c-Met expression in combination with the co-expression of hepatocyte growth factor (HGF) in the epithelial cells of inverted papilloma may proliferate the epithelial cells of inverted papilloma.


OBJECTIVE
HGF and its receptor, c-Met, have been identified in a variety of neoplastic and normal tissue types, implicating these factors in tissue regeneration and tumor progression. We investigated the expression and distribution of HGF and c-Met in normal nasal mucosa and inverted papilloma, to evaluate the possible influence of HGF and c-Met on the development of inverted papilloma.


METHODS
Normal inferior turbinate mucosa and inverted papilloma were examined for expression of HGF and c-Met using RT-PCR, immunohistochemistry and Western blot analysis.


RESULTS
Using immunohistochemistry, moderate to high levels of HGF and c-Met protein were localized in epithelial cells in inverted papillomas tested in the present study. In normal turbinate mucosa immunopositive HGF was detected in the submucosal glands where faint staining was found. However, c-Met was noted in the epithelial cells and submucosal glands of normal turbinate mucosa. RT-PCR and Western blot analysis showed that the expression levels of HGF and c-Met were increased in inverted papilloma in comparison with the normal turbinate mucosa.
Choi
Ji Hoon
JH
Department of Otorhinolaryngology-Head & Neck Surgery, College of Medicine, Korea University, Seoul, Korea.
Lee
Sang Hag
SH
Lee
Heung Man
HM
Lee
Seung Hoon
SH
Jung
Hak Hyun
HH
Choi
Joon
J
Lim
Hyun Woo
HW
Lee
Se Woo
SW
Kang
Sun Mook
SM
D000328:Adult; D015153:Blotting, Western; D005260:Female; D015870:Gene Expression; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism* / Q000473:pathology; D018308:Papilloma, Inverted / Q000378:metabolism* / Q000473:pathology; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met