[Inhibition of rhodiola on the growth of EVC-304 cell line].
OBJECTIVE
To observe the effect of rhodiola on human umbilical vein endothelial cell line EVC-304.


METHODS
EVC-304 was cultured and divided into two groups: control group and rhodiola-treated group. Three days after treatment, cell survival rate-drug concentration curve was obtained by counting the survival cells, and cells in each group were stained by Wright's stain and observed under microscope. Cell cycle was determined by flow cytometry (FCM).


RESULTS
The survival cells in rhodiola-treated group was much less than those in control group. More cells in rhodiola-treated group stayed in G(1) phase while less in S phase when compared with those in control group by FCM.


CONCLUSIONS
Rhodiola can inhibit the growth of human endothelial cell line EVC-304, perhaps through inhibiting the proliferation of the cells. This may lay the foundation for the mechanism study and clinical application of rhodiola in prevention of pulmonary artery hypertension.
Sui
Xiu-lan
XL
Second People Hospital of Tibet, Lhasa 850002, China. sui@public.ls.xz.cn
Yang
Feng
F
Chen
Rong-hua
RH
Ga
Bu
B
Yan
Hui
H
Zhang
Shi-fu
SF
Zhang
Jing-ling
JL
D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D006976:Hypertension, Pulmonary / Q000473:pathology / Q000517:prevention & control; D029771:Rhodiola; D014471:Umbilical Veins / Q000166:cytology