title: Genome-wide survey of the gene expression response to saprolegniasis in Atlantic salmon.
abstract: Pathogenic saprolegniaceae species are among the major disease-causing agents in farmed salmonids and in freshwater fish in general. Recent studies have used high-throughput cDNA-based methods to identify new potential actors of fish defence systems against various bacteria and viruses. However, the response of fish to fungal or fungus-like pathogens is still poorly documented. Here, we used a 16,006-gene salmonid cDNA microarray to identify genes which transcription levels are modified in juvenile Atlantic salmon (Salmo salar) affected with saprolegniasis compared to healthy fish from the same families. Our results confirmed the importance of non-specific immunity in the response of fish to saprolegniaceae infections and identified both similarities and differences in their genome-wide transcriptional response to oomycetes compared with their responses to bacterial or viral infections. Moreover, several clones with no known homologues were shown to be over-transcribed in infected fish. These may represent as yet unidentified immune-relevant genes in fish.
authors.affiliation: Département de Biologie, Université Laval, Québec, Canada G1K 7P4. Christian.Roberge@giroq.ulaval.ca
authors.forename: Christian
authors.initials: C
authors.lastname: Roberge
authors.forename: David J
authors.initials: DJ
authors.lastname: Páez
authors.forename: Orlane
authors.initials: O
authors.lastname: Rossignol
authors.forename: Helga
authors.initials: H
authors.lastname: Guderley
authors.forename: Julian
authors.initials: J
authors.lastname: Dodson
authors.forename: Louis
authors.initials: L
authors.lastname: Bernatchez
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D016678:Genome / Q000276:immunology*; D007239:Infections / Q000235:genetics / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D020317:Salmo salar / Q000235:genetics* / Q000276:immunology* / Q000469:parasitology; D044762:Saprolegnia / Q000235:genetics / Q000276:immunology*