title: [Kinetic study of various cytokine mRNA expressions in rhesus treated with haploidentical peripheral blood stem cell transplantation].
abstract: This study was aimed to analyze the mRNA expression of cytokines (TGF-beta, IL-2, IL-6, IL-10, IFN-gamma, TNF-alpha, FAS-L) in five rhesus treated with haploidentical peripheral blood stem cell transplantation after nonmyeloablative preparative regimens and to explore the role of these cytokines in the development and pathology of acute graft-versus-host-disease (aGVHD). Five rhesus monkeys received nonmyeloablative haploidentical peripheral blood stem cells transplantation. Semi-quantitative reversed transcription polymerase chain reaction (RT-PCR) was used to analyze the kinetics of cytokine mRNA expression in the transplantation and aGVHD. The results showed that five rhesus monkeys acquired hematopoietic reconstitution successfully. The graft was rejected in one monkey which survived without disease, the other four achieved mixed chimerism and full donor chimerism. Chimerism of low centigrade in one monkey achieved high centigrade at 35 days after donor stem cell infusion. Intestinal aGVHD grade III developed in one monkey. Cytokines of Th1 and Th2 changed after transplantation. In period of aGVHD, expression of TGF-beta decreased but all others increased in various levels. When donor chimerism decreased, the cytokines decreased accordingly. It is concluded that the decrease of TGF-beta mRNA may be an indicator to predict aGVHD, and can be used as a differential diagnostic indicator for intestinal GVHD.
authors.affiliation: Department of Hematology, Hospital Affiliated to Academy Military Medical Sciences, Beijing 100071, China.
authors.forename: Ya-Jing
authors.initials: YJ
authors.lastname: Huang
authors.forename: Qi-Yun
authors.initials: QY
authors.lastname: Sun
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Liu
authors.forename: Kai-Xun
authors.initials: KX
authors.lastname: Hu
authors.forename: Chuan-Bo
authors.initials: CB
authors.lastname: Fan
authors.forename: Li
authors.initials: L
authors.lastname: Bian
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
authors.forename: Hui-Sheng
authors.initials: HS
authors.lastname: Ai
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000378:metabolism*; D006238:Haploidy; D008253:Macaca mulatta; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta