Co-expression of interleukin-6 and human growth hormone in apparently normal prostate biopsies that ultimately progress to prostate cancer using low pH, high temperature antigen retrieval.
Prostate cancer is the most common cancer in American men and the second leading cause of cancer deaths in this group. Both growth hormone (GH) and the inflammatory cytokine interleukin 6 (IL-6) have been implicated in prostate cancer progression. Studies in other systems have shown that an increase in GH results in an increase in IL-6 also. The current study demonstrated a parallel spatial and temporal expression of GH and IL-6 in cells in prostate cancer glandular acina cells. This study cannot determine if this expression is coincidental or causative, but it seems likely that the increase in GH could induce the expression of IL-6, since this is the case in other tissues. Optimal labelling for IL-6 in our study was achieved with low pH, high temperature antigen retrieval.
Slater
M D
MD
School of Medical Sciences, Department of Anatomy and Histology F13, The University of Sydney, Sydney, NSW 2006, Australia. michaels@anatomy.usyd.edu.au
Murphy
C R
CR
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000941:Antigens / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect; D006358:Hot Temperature; D019382:Human Growth Hormone / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000033:anatomy & histology / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology
D000941:Antigens; D015850:Interleukin-6; D019382:Human Growth Hormone