title: Acetic acid-guided biopsies after magnifying endoscopy compared with random biopsies in the detection of Barrett's esophagus: a prospective randomized trial with crossover design.
abstract: BACKGROUND
  In contrast to standard video endoscopy, magnifying endoscopy after local acetic acid application enables recognition of mucosal surface architecture.
  
  
  OBJECTIVE
  To investigate the diagnostic yield of magnifying endoscopy with acetic acid-targeted biopsies compared to random, 4-quadrant biopsies.
  
  
  METHODS
  Prospective randomized trial (ratio 1:1) with crossover design.
  
  
  METHODS
  Two referral hospitals in Germany.
  
  
  METHODS
  Thirty-one patients with Barrett's esophagus or visible columnar-lined lower esophagus.
  
  
  METHODS
  Patients were randomized to undergo either standard video endoscopy with 4-quadrant biopsies or magnifying endoscopy in conjunction with acetic acid application. All patients were re-examined 14 days after the initial endoscopy with the corresponding procedure.
  
  
  METHODS
  Primary outcome analysis (per protocol and per biopsy) was the histological proof of Barrett's epithelium. Secondary outcome analysis was the correlation between the surface architecture and the presence of Barrett's epithelium and the needed number of biopsies to confirm Barrett's epithelium for the 2 different procedures.
  
  
  RESULTS
  Magnifying endoscopy enabled the prediction of Barrett's epithelium with a sensitivity of 100% and a specificity of 66%, respectively (accuracy 83.8%). Acetic acid-guided biopsies obtained a significantly higher percentage of tissues containing SCE (78%; 188/241) compared to random biopsies (57%; 159/280).
  
  
  CONCLUSIONS
  No data on the diagnosis of Barrett's dysplasia.
  
  
  CONCLUSIONS
  Magnifying endoscopy with acetic acid-guided biopsies is superior to standard video endoscopy with random biopsies, and the number of biopsies needed to confirm Barrett's epithelium is half as much when compared to random biopsies.
authors.affiliation: I Med. Clinic und Poliklinik, Institute for Statistics, Johannes Gutenberg University Mainz, Langenbeckstrasse 1, 55101 Mainz, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Hoffman
authors.forename: R
authors.initials: R
authors.lastname: Kiesslich
authors.forename: A
authors.initials: A
authors.lastname: Bender
authors.forename: M F
authors.initials: MF
authors.lastname: Neurath
authors.forename: B
authors.initials: B
authors.lastname: Nafe
authors.forename: G
authors.initials: G
authors.lastname: Herrmann
authors.forename: M
authors.initials: M
authors.lastname: Jung
mesh_terms: D019342:Acetic Acid; D000293:Adolescent; D000328:Adult; D000368:Aged; D001471:Barrett Esophagus / Q000473:pathology*; D001706:Biopsy / Q000379:methods; D003116:Color; D018592:Cross-Over Studies; D016099:Endoscopy, Gastrointestinal / Q000379:methods*; D004947:Esophagus / Q000473:pathology; D005260:Female; D006801:Humans; D007089:Image Enhancement; D007202:Indicators and Reagents; D008297:Male; D008679:Metaplasia; D008853:Microscopy; D008875:Middle Aged; D009092:Mucous Membrane / Q000473:pathology; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D007202:Indicators and Reagents; D019342:Acetic Acid