Reidentification of cellulolytic enzyme-producing Trichoderma strains W-10 and G-39.
Strain W-10, originally identified as Trichoderma koningii, and its supposed mutant G-39, published for production and gene expression of cellulase and xylanase, demonstrated morphological characteristics distinct from those of T. koningii, respectively. To clarify the identification derived from morphological characteristics, several methods were used, including electrophoretic karyotyping, internal transcribed spacer (ITS) analysis of rDNA, and polymerase chain reaction (PCR) fingerprinting using the universal primer L45. All the molecular characteristics showed that strains G-39 and W-10 were identical to T. reesei and T. longibrachiatum, respectively. The results strongly supported that T. koningii G-39 and W-10 should be reassigned as T. reesei and T. longibrachiatum, respectively. Strain G-39 should be considered a mutant from T. reesei QM9414 whose spores were contaminated with those of strain W-10 during a laboratory operation. According to this, we declare that T. koningii G-39 and W-10 must be renamed as T. reesei and T. longibrachiatum, respectively.
Lee
Ching-Fu
CF
Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan. Lshtm@life.nthu.edu.tw
Liu
Daniel Yuen Teh
DY
Lai
Ming Tsong
MT
Hseu
Tzong-Hsiung
TH
D002480:Cellulase / Q000096:biosynthesis / Q000378:metabolism*; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004586:Electrophoresis / Q000379:methods; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D014242:Trichoderma / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D014995:Xylosidases / Q000378:metabolism
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D014995:Xylosidases; D002480:Cellulase