title: Sucrose, glucose, and fructose extraction in aqueous carrot root extracts prepared at different temperatures by means of direct NMR measurements.
abstract: Solutions obtained by heating carrot roots in water (stocks) are widely used in the food industry, but little information is available regarding the metabolites (intermediates and products of metabolism) found in the stock. The effect of treatment temperature and duration on the sugar composition of stocks was investigated directly by quantitative (1)H NMR spectroscopy, to understand the extraction mechanism when processing at 100 degrees C. Stocks prepared at three different temperatures (50, 75, and 100 degrees C) were investigated for up to 36 h. Three sugars (sucrose, glucose, and fructose) were detected and quantified. The concentrations of these three sugars reached a maximum after 9 h when the temperature of treatment was 50 or 75 degrees C. At 100 degrees C, the sucrose concentration reached a maximum after 3 h, whereas the concentration of glucose and fructose was still increasing at that time. Comparison of the kinetic composition of these carrot stocks with that of model sugar solutions leads to the proposal that the changes in stock composition result from sugar diffusion, sucrose hydrolysis, and hydroxymethylfurfural (HMF) formation.
authors.affiliation: Diana Ingrédients, BP 244, 56007 Vannes, France. anne.cazor@colege-de-france.fr
authors.forename: Anne
authors.initials: A
authors.lastname: Cazor
authors.forename: Catherine
authors.initials: C
authors.lastname: Deborde
authors.forename: Annick
authors.initials: A
authors.lastname: Moing
authors.forename: Dominique
authors.initials: D
authors.lastname: Rolin
authors.forename: Hervé
authors.initials: H
authors.lastname: This
mesh_terms: D018552:Daucus carota / Q000737:chemistry*; D005632:Fructose / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D013395:Sucrose / Q000032:analysis*; D013696:Temperature
chemical_list: D010936:Plant Extracts; D005632:Fructose; D013395:Sucrose; D005947:Glucose