title: Structural features in carrageenan that interact with a heparin-binding hematopoietic growth factor and modulate its biological activity.
abstract: The effects of carrageenans' structural features on its interaction with granulocyte colony-stimulating factor (G-CSF) and on the growth and differentiation of a G-CSF dependent leukemia cell line (NFS-60) were studied. lambda, iota, and kappa carrageenans, with decreasing contents of sulfation, bound to G-CSF with binding constants of (6.2+/-0.6) x 10(5)M(-1), (7.4+/-0.5) x 10(5)M(-1) and (6.0+/-0.4) x 10(5)M(-1), and with 27.7+/-0.2, 17.4+/-0.1 and 8.4+/-0.1 binding sites, respectively. However, kappa carrageenan oligosaccharide had no affinity for G-CSF. The three carrageenans significantly inhibited G-CSF-induced growth of NFS-60 cells. The high sulfate content lambda carrageenan could also induce the maturation of the cells, but relatively low sulfate content iota and kappa carrageenans could not. The results suggested that G-CSF-carrageenan bindings were dependent on carrageenans' sulfate contents and chain lengths, which could also affect the growth and differentiation of NFS-60 cells.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
authors.forename: Aiye
authors.initials: A
authors.lastname: Liang
authors.forename: Xiaomian
authors.initials: X
authors.lastname: Zhou
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Liu
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
authors.forename: Keyi
authors.initials: K
authors.lastname: Wang
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002351:Carrageenan / Q000737:chemistry / Q000378:metabolism*; D002453:Cell Cycle; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019075:Electrophoresis, Capillary; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D006801:Humans
chemical_list: D016179:Granulocyte Colony-Stimulating Factor; D002351:Carrageenan; D006493:Heparin