title: nDsbD: a redox interaction hub in the Escherichia coli periplasm.
abstract: DsbD is a redox-active protein of the inner Escherichia coli membrane possessing an N-terminal (nDsbD) and a C-terminal (cDsbD) periplasmic domain. nDsbD interacts with four different redox proteins involved in the periplasmic disulfide isomerization and in the cytochrome c maturation systems. We review here the studies that led to the structural characterization of all soluble DsbD domains involved and, most importantly, of trapped disulfide intermediate complexes of nDsbD with three of its four redox partners. These results revealed the structural features enabling nDsbD, a 'redox hub' with an immunoglobulin-like fold, to interact efficiently with its different thioredoxin-like partners.
authors.affiliation: Biochemisches Institut, Universität Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland.
authors.forename: C U
authors.initials: CU
authors.lastname: Stirnimann
authors.forename: M G
authors.initials: MG
authors.lastname: Grütter
authors.forename: R
authors.initials: R
authors.lastname: Glockshuber
authors.forename: G
authors.initials: G
authors.lastname: Capitani
mesh_terms: D020816:Amino Acid Motifs; D003545:Cysteine / Q000737:chemistry / Q000502:physiology; D003553:Cystine / Q000737:chemistry / Q000502:physiology; D019281:Dimerization; D004579:Electron Transport; D004789:Enzyme Activation; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000502:physiology*; D019897:Periplasm / Q000378:metabolism; D033903:Periplasmic Proteins / Q000502:physiology; D011487:Protein Conformation; D011490:Protein Disulfide Reductase (Glutathione) / Q000378:metabolism; D019704:Protein Disulfide-Isomerases / Q000502:physiology; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship; D013879:Thioredoxins / Q000737:chemistry
chemical_list: D029968:Escherichia coli Proteins; D033903:Periplasmic Proteins; D003553:Cystine; D013879:Thioredoxins; D009249:NADP; D010088:Oxidoreductases; C094848:DsbD electron transport protein, E coli; C109831:DsbG protein, E coli; C485980:Ccmg protein, E coli; D011490:Protein Disulfide Reductase (Glutathione); D019704:Protein Disulfide-Isomerases; D003545:Cysteine