Evaluation of RGD modification on collagen matrix.
OBJECTIVE
To observe the changes during the process of modificating collagen with Arg-Gly-Asp (RGD) peptide, a series experiments were designed.


METHODS
The 3D porous collagen matrices modified with RGD peptide were constructed by lyophilization. The modified and unmodified matrices were characterized by Scanning Electron Microscopy (SEM) and Electron Spectroscopy for Chemical Analysis (ESCA). Fibroblasts were used to evaluate the cell compatibility of the matrices.


RESULTS
In terms of cell growth, the cells attached much better on the modified matrix than on the unmodified one. Compare to the unmodified matrices, the polar groups on the modified matrix increased.


CONCLUSIONS
The introducing of specific RGD receptor-mediated adhesion site on matrices obviously enhanced the cells adhesion on collagen matrices.
Ren
Dongfeng
D
Department of Orthopaedics, Institute of Orthopaedics, Beijing, PR China.
Hou
Shuxun
S
Wang
Huadong
H
Luo
Dianzhong
D
Zhang
Lihai
L
D000042:Absorption; D000596:Amino Acids / Q000032:analysis; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering; D014867:Water
D000596:Amino Acids; D001672:Biocompatible Materials; D009842:Oligopeptides; D014867:Water; C047981:arginyl-glycyl-aspartic acid; D003094:Collagen