The effect of adenosine 5'-triphosphate on calcium mobilization and cell proliferation in cervical cancer cells.
OBJECTIVE
To know the effect of adenosine 5'-triphosphate (ATP) on intracellular calcium level and cell proliferation in cervical cancer cells.


METHODS
Four different human cervical cancer cell lines (Caski, C33A, HeLaS3 and SiHa) were used in this study. The change of intracellular calcium level, cell proliferation and the activity of proliferation- and calcium-related transcription factors by extracellular ATP were examined in these cell lines.


RESULTS
Extracellular ATP induced calcium mobilization, cell proliferation and the activation of NF-kappaB in all cell lines used.


CONCLUSIONS
These results suggest that calcium mobilization and NF-kappaB dependent signaling pathway play an important role in the cell proliferation by ATP in cervical cancer.
Lee
Seok Geun
SG
Catholic Neuroscience Center, Catholic Research Institutes of Medical Science, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
Choi
Jung-Kyoung
JK
Choi
Byung Hyune
BH
Lim
Young
Y
Kim
Young-Hoon
YH
Lee
Kweon-Haeng
KH
Shin
Jong Chul
JC
Ahn
Woong Shick
WS
D000230:Adenocarcinoma / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
D016328:NF-kappa B; D000255:Adenosine Triphosphate; D002118:Calcium