title: Thiobarbituric acid reactive substances, Fe3+ reduction and enzymatic activities in cultures of Ganoderma australe growing on Drimys winteri wood.
abstract: Ganoderma australe is a basidiomycete responsible for a natural process of selective and extensive lignin degradation. Fatty acids, thiobarbituric acid reactive substances (TBARS), Fe3+-reduction and enzymatic activities were monitored in cultures of G. australe growing on Drimys winteri wood chips. Linoleic acid was de novo synthesized, and steadily increased during 12 weeks of cultivation. Part of the unsaturated fatty acids underwent peroxidation as TBARS accumulated with biodegradation time. TBARS accumulation was proportional to the wood weight and component losses. Manganese-dependent peroxidase and lignin peroxidase were not detected in the culture extracts, whereas laccase-induced oxidation of syringaldazine peaked after 2 weeks (104+/-9 micromol oxidized min(-1) kg(-1) of dry wood), subsequently decreasing. On the other hand, nonenzymatic Fe3+-reducing activity increased as a function of cultivation time and could be involved in the initiation of lipid peroxidation.
authors.affiliation: Renewable Resources Laboratory, Biotechnology Center, Universidad de Concepción, Concepción, Chile.
authors.forename: Juan-Pedro
authors.initials: JP
authors.lastname: Elissetche
authors.forename: André
authors.initials: A
authors.lastname: Ferraz
authors.forename: Juanita
authors.initials: J
authors.lastname: Freer
authors.forename: Régis
authors.initials: R
authors.lastname: Mendonça
authors.forename: Jaime
authors.initials: J
authors.lastname: Rodríguez
mesh_terms: D001673:Biodegradation, Environmental; D032424:Drimys / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism*; D032902:Ganoderma / Q000254:growth & development / Q000378:metabolism*; D015227:Lipid Peroxidation; D017392:Thiobarbituric Acid Reactive Substances; D014934:Wood
chemical_list: D005290:Ferric Compounds; D017392:Thiobarbituric Acid Reactive Substances