title: Stereoselective incorporation of isoleucine into Cypridina luciferin in Cypridina hilgendorfii (Vargula hilgendorfii).
abstract: The emission of light in the marine ostracod Cypridina hilgendorfii (presently Vargula hilgendorfii) is produced by the Cypridina luciferin-luciferase reaction in the presence of molecular oxygen. Cypridina luciferin has an asymmetric carbon derived from isoleucine, and the absolute configuration is identical to the C-3 position in L-isoleucine or D-alloisoleucine. To determine the stereoselective incorporation of the isoleucine isomers (L-isoleucine, D-isoleucine, L-alloisoleucine, and D-alloisoleucine), we synthesized four (2)H-labeled isoleucine isomers and examined their incorporation into Cypridina luciferin by feeding experiments. Judging by these results, L-isoleucine is predominantly incorporated into Cypridina luciferin. This suggests that the isoleucine unit of Cypridina luciferin is derived from L-isoleucine, but not from D-alloisoleucine.
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kato
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Oba
authors.forename: Makoto
authors.initials: M
authors.lastname: Ojika
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inouye
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003445:Crustacea / Q000145:classification* / Q000378:metabolism*; D007532:Isoleucine / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D013237:Stereoisomerism; D013844:Thiazoles / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D013844:Thiazoles; D007532:Isoleucine