Pro-antibiotic substrates for the identification of enantioselective hydrolases.
A new growth-based screening method for the identification of enantioselective hydrolases, such as lipases and esterases, using pro-antibiotic substrates was devised. An enantioselective hydrolase could be identified by measuring growth rates of cells in liquid media containing (R)- or (S)-2-phenylbutyric chloramphenicol esters. This method can be applied to the screening of novel enantioselective microbes and to the high-throughput screening for the directed evolution of enantioselective hydrolytic enzymes.
Hwang
Bum-Yeol
BY
School of Chemical and Biological Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742, Korea.
Oh
Jong-Min
JM
Kim
Juhan
J
Kim
Byung-Gee
BG
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001406:Bacillaceae / Q000254:growth & development; D001681:Biological Assay / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D004950:Esterases / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D004952:Esters / Q000378:metabolism; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods; D008049:Lipase / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D013237:Stereoisomerism; D013379:Substrate Specificity / Q000502:physiology
D000900:Anti-Bacterial Agents; D004952:Esters; D004950:Esterases; D008049:Lipase