title: In vivo assays for transcription-coupled repair.
abstract: This chapter describes the technologies used in our respective laboratories to study the incidence and repair of lesions induced in specific DNA sequences by ultraviolet light, chemical carcinogens, and products of cellular metabolism. The Southern blot method is suitable for analysis of damage and repair in the individual DNA strands of specific restriction fragments up to 25,000 nucleotides in length, whereas the ligation-mediated polymerase chain reaction approach permits analysis of shorter sequences at the nucleotide level. Both methods have unique advantages and limitations for particular applications.
authors.affiliation: Department of Biological Sciences, Stanford University, California, USA.
authors.forename: Graciela
authors.initials: G
authors.lastname: Spivak
authors.forename: Gerd P
authors.initials: GP
authors.lastname: Pfeifer
authors.forename: Philip
authors.initials: P
authors.lastname: Hanawalt
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D006801:Humans; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
chemical_list: D004247:DNA