title: Redox mechanisms of vascular cell dysfunction in sepsis.
abstract: Sepsis remains one of the leading causes of death in intensive care units, despite recent acquired knowledge on pathophysiology and treatment. Several mediators of inflammation and cellular damage have been implicated in the complex host-pathogen interaction underlying organ damage and multisystem organ failure , which are hallmarks of sepsis and common causes of death. Among such mediators, reactive oxygen/nitrogen species have been increasingly studied in the context of direct cytotoxicity as well as altered cell signaling. While the generation of reactive oxygen species by inflammatory cells in sepsis is well known, recent studies have shown that vascular cells are able to release reactive oxygen intermediates that may be associated with endothelial dysfunction of sepsis. These compounds can activate transcription factors such as NF-kappaB that sustain inflammatory process or enzymatic systems like poly(ADP-ribose) polymerase-1, which are involved in apoptosis and cytotoxicity of sepsis. Our laboratory recently showed that platelet-derived exosomes from septic patients carry components of a superoxide-producing NADPH oxidase and can, at least in vitro, induce apoptosis of endothelial and vascular smooth muscle cells by a ROS-dependent pathway. Taken together, these data show that reactive oxygen species are involved in cell signaling and organ injury in sepsis. Efforts must be made to identify the precise contribution of these factors in septic process, in order to clarify the mechanisms associated with the disease. This will certainly lead to discovery of therapeutic strategies that can help us to mitigate vascular dysfunction of sepsis.
authors.affiliation: Emergency Medicine Discipline, University of São Paulo Medical School, São Paulo, Brazil. lucianoazevedo@uol.com.br
authors.forename: L C P
authors.initials: LC
authors.lastname: Azevedo
authors.forename: M
authors.initials: M
authors.lastname: Janiszewski
authors.forename: F G
authors.initials: FG
authors.lastname: Soriano
authors.forename: F R M
authors.initials: FR
authors.lastname: Laurindo
mesh_terms: D000818:Animals; D042783:Endothelial Cells / Q000378:metabolism* / Q000382:microbiology / Q000502:physiology; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D018805:Sepsis / Q000378:metabolism* / Q000382:microbiology
chemical_list: D005609:Free Radicals; D017382:Reactive Oxygen Species