Morphological classification of parvalbumin-containing retinal ganglion cells in mouse: single-cell injection after immunocytochemistry.
OBJECTIVE
Matching the neuron's morphology with its expression of a particular protein cannot be easily achieved by immunocytochemistry alone, as many proteins are expressed too weakly. In this study, a newly developed method was adopted to match mouse retinal ganglion cell (RGC) morphology with its expression of parvalbumin.


METHODS
Parvalbumin-containing ganglion cells were first identified by immunocytochemistry and then were iontophoretically injected with a lipophilic dye DiI. Then confocal microscopy was used to characterize the morphologic classification of the parvalbumin-immunoreactive (IR) ganglion cells on the basis of the dendritic field size, branching pattern, and stratification within the inner plexiform layer.


RESULTS
The results indicate that at least eight different types of ganglion cells express parvalbumin in the mouse retina. They were heterogeneous in morphology: monostratified to bistratified, small-to-large dendritic field size, and sparse-to-dense dendritic arbors.


CONCLUSIONS
Single-cell injection, after immunocytochemistry, provided the first means to identify the detailed functional anatomy of parvalbumin-containing RGCs in the mouse retina. The combined approach of cell morphology and the selective expression of parvalbumin will not only provide useful data for further correlation of physiological properties of the RGCs, but it will also provide a useful strategy for matching a neuron's morphology with its expression of a particular protein.
Kim
Tae-Jin
TJ
Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, Korea.
Jeon
Chang-Jin
CJ
D000818:Animals; D002232:Carbocyanines / Q000008:administration & dosage; D002452:Cell Count; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000008:administration & dosage; D007267:Injections; D007478:Iontophoresis; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D010320:Parvalbumins / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000378:metabolism*
C035005:3,3'-dihexadecylindocarbocyanine; D002232:Carbocyanines; D005456:Fluorescent Dyes; D010320:Parvalbumins