Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages.
Performing patch-clamp experiments on human macrophages, we show that the K(+) channel MaxiK is activated by lipopolysaccharide, peptidoglycan, and interleukin-1. Cytokine production initiated by several Toll-like receptor (TLR) ligands and by interleukin-1 is inhibited by MaxiK blockade. This provides evidence for functional association of the MaxiK channel and TLR signaling complexes.
Scheel
Olaf
O
Research Center Borstel, Department of Immunochemistry and Biochemical Microbiology, Division of Biophysics, Parkallee 10, 23845 Borstel, Germany.
Papavlassopoulos
Martin
M
Blunck
Rikard
R
Gebert
Andreas
A
Hartung
Thomas
T
Zähringer
Ulrich
U
Seydel
Ulrich
U
Schromm
Andra B
AB
D002460:Cell Line; D006801:Humans; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits / Q000502:physiology*; D008024:Ligands; D008262:Macrophage Activation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D005810:Multigene Family; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000276:immunology; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*
C494841:KCNMA1 protein, human; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D008024:Ligands; D017472:Receptors, Interleukin-1; D051193:Toll-Like Receptors