title: Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses.
abstract: A synthetic chimeric gene, TBI-HBS, encoding the immunogenic ENV and GAG epitopes of human immunodeficiency virus (HIV-1) and the surface protein antigen (HBsAg) of hepatitis B virus (HBV), was expressed in tomato plants. Tomato fruits containing the TBI-HBS antigen were fed to experimental mice and, on days 14 and 28 post-feeding, high levels of HIV- and HBV-specific antibodies were present in the serum and feces of the test animals. Intraperitoneal injection of a DNA vaccine directing synthesis of the same TBI-HBsAg antigen boosted the antibody response to HIV in the blood serum; however, it had no effect on the high level of antibodies produced to HBV.
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region, 630559, Russia.
authors.forename: Sergei N
authors.initials: SN
authors.lastname: Shchelkunov
authors.forename: Rurik K
authors.initials: RK
authors.lastname: Salyaev
authors.forename: Sergei G
authors.initials: SG
authors.lastname: Pozdnyakov
authors.forename: Natalia I
authors.initials: NI
authors.lastname: Rekoslavskaya
authors.forename: Andrei E
authors.initials: AE
authors.lastname: Nesterov
authors.forename: Tatiana S
authors.initials: TS
authors.lastname: Ryzhova
authors.forename: Valentina M
authors.initials: VM
authors.lastname: Sumtsova
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Pakova
authors.forename: Uliana O
authors.initials: UO
authors.lastname: Mishutina
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Kopytina
authors.forename: Rosemarie W
authors.initials: RW
authors.lastname: Hammond
mesh_terms: D000284:Administration, Oral; D017325:Hepatitis B Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism*; D006515:Hepatitis B virus / Q000187:drug effects* / Q000502:physiology; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000276:immunology*; D030821:Plants, Genetically Modified; D015202:Protein Engineering / Q000379:methods
chemical_list: D017325:Hepatitis B Vaccines; D010936:Plant Extracts