title: Roles of the intramolecular disulfide bridge in MotX and MotY, the specific proteins for sodium-driven motors in Vibrio spp.
abstract: The proteins PomA, PomB, MotX, and MotY are essential for the motor function of Na+-driven flagella in Vibrio spp. Both MotY and MotX have the two cysteine residues (one of which is in a conserved tetrapeptide [CQLV]) that are inferred to form an intramolecular disulfide bond. The cysteine mutants of MotY prevented the formation of an intramolecular disulfide bond, which is presumably important for protein stability. Disruption of the disulfide bridge in MotX by site-directed mutagenesis resulted in increased instability, which did not, however, affect the motility of the cells. These lines of evidence suggest that the intramolecular disulfide bonds are involved in the stability of both proteins, but only MotY requires the intramolecular bridge for proper function.
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-Ku, Nagoya 464-8602, Japan.
authors.forename: Jin
authors.initials: J
authors.lastname: Yagasaki
authors.forename: Mayuko
authors.initials: M
authors.lastname: Okabe
authors.forename: Rie
authors.initials: R
authors.lastname: Kurebayashi
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yakushi
authors.forename: Michio
authors.initials: M
authors.lastname: Homma
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D003545:Cysteine; D004220:Disulfides / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry; D010957:Plasmids; D012964:Sodium / Q000378:metabolism*; D014733:Vibrio / Q000235:genetics / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004220:Disulfides; D008565:Membrane Proteins; C089633:MotX protein, bacteria; C088135:MotY protein, Vibrio; D009842:Oligopeptides; D012964:Sodium; D003545:Cysteine