title: [Laboratory diagnoses of the first confirmed human case of avian influenza A (H5N1) in the mainland of China].
abstract: BACKGROUND
  To determine the etiologic agent of an atypical pneumonia human case admitted to Xiangtan City hospital, Hunan Province in Oct. 2005.
  
  
  METHODS
  The patient's respiratory tract samples and serum were collected. Throat swabs were tested by microneutralization and hemagglutination-inhibition assays.
  
  
  RESULTS
  The results of nucleic acid detection of all respiratory samples were negative and virus isolation was also negative. The H5-specific antibodies of convalescence showed a 4-fold greater rise than acute phase.
  
  
  CONCLUSIONS
  The atypical pneumonias case was confirmed as the first human case of avian influenza A (H5N1) infection in the mainland of China.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Control and Prevention, Beijing 100052, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Yun-zhi
authors.initials: YZ
authors.lastname: Liu
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Hong-jie
authors.initials: HJ
authors.lastname: Yu
authors.forename: Wei-zhong
authors.initials: WZ
authors.lastname: Yang
authors.forename: Cui-ling
authors.initials: CL
authors.lastname: Xu
authors.forename: Ni-juan
authors.initials: NJ
authors.lastname: Xiang
authors.forename: Zhong-jie
authors.initials: ZJ
authors.lastname: Li
authors.forename: Yu-xu
authors.initials: YX
authors.lastname: Chen
authors.forename: Yuan-ji
authors.initials: YJ
authors.lastname: Guo
authors.forename: De-xin
authors.initials: DX
authors.lastname: Li
authors.forename: Jun-hua
authors.initials: JH
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Yue-long
authors.initials: YL
authors.lastname: Shu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D002642:Chick Embryo; D002648:Child; D002681:China; D019411:Clinical Laboratory Techniques; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D008297:Male; D009500:Neutralization Tests; D011024:Pneumonia, Viral / Q000175:diagnosis / Q000821:virology; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011933:Reagent Kits, Diagnostic