ZNF569, a novel KRAB-containing zinc finger protein, suppresses MAPK signaling pathway.
Transcription factors play an essential role in altering gene expression. A great progress about transcription factors has been made towards the understanding of normal physiological processes, embryonic development, and human diseases. Here we report the identification and characterization of a novel KRAB-containing zinc-finger protein, ZNF569, which is isolated from a human embryonic heart cDNA library. ZNF569 encodes a putative protein of 686 amino acids. The protein is conserved across different species during evolution. Expression of ZNF569 was found in most of the examined human adult and embryonic tissues with a higher level in heart and skeletal muscles. The KRAB and ZNF motifs of ZNF569 represent potent repression domains. When ZNF569 is fused to Gal-4 DNA-binding domain and co-transfected with VP-16, ZNF569 protein suppresses transcriptional activity. Overexpression of ZNF569 in COS-7 cells inhibited the transcriptional activities of SRE and AP-1, which may be silenced by siRNA. The results suggest that ZNF569 protein may act as a transcriptional repressor that suppresses MAPK signaling pathway to mediate cellular functions.
Huang
Xinqiong
X
The Center for Heart Development, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, People's Republic of China.
Yuan
Wuzhou
W
Huang
Wen
W
Bai
Yan
Y
Deng
Yun
Y
Zhu
Chuanbing
C
Liang
Pei
P
Li
Yongqing
Y
Du
Xiaoyan
X
Liu
Mingyao
M
Wang
Yuequn
Y
Wu
Xiushan
X
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D016153:Genes, Suppressor; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D020935:MAP Kinase Signaling System; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D018808:Transcription Factor AP-1; C512510:ZNF569 protein, human