Protective role of 3-nitro-N-methyl-salicylamide on isolated rat heart during 4 hours of cold storage and reperfusion.
OBJECTIVE
Cardiac ischemia/reperfusion (I/R) injury, a necessary consequence of transplantation, is probably related to the formation of reactive oxygen species (ROS). The ROS burst within the first moments of reperfusion is associated with injury, continuously generate O2- at about 3% to 5% of total O2 consumption owing to electron leak by mitochondrial oxidoreductases, especially complexes I and III. 3-nitro-N-methyl-salicylamide (NNMS) displays inhibitory effects on succinate-cytochrome C reductase, but also reduces effects on creation of O2- radical and H2O2 by isolated rat mitochondria. Presumably NNMS inhibits electron leakage from the mitochondrial respiratory chain. We investigated effect of NNMS on heart protection after hypothermic ischemia.


METHODS
A Langendorff-prepared rat heart model was employed after the heart had been preserved for 4 hours under hypothermic conditions of ischemia with subsequent reperfusion/rewarming for 60 minutes.


RESULTS
The group of hearts treated with NNMS showed increased recovery of heart function compared with a group of mEC. The lactate dehydrogenase (LDH) activity in coronary flow (CF) by hearts treated with NNMS was lower than that with mECs, as was the content of malonedialdehyde (MDA) and conjugated diene (CD).


CONCLUSIONS
NNMS improved heart physiology after reperfusion following 4 hours of hypothermic ischemia.
Zhang
S
S
National Laboratory of Biomacromolecule, Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, China.
Yang
J H
JH
Yu
F
F
Zhao
J
J
Jiang
P
P
Chang
L
L
Tang
C
C
Xu
J
J
D000818:Animals; D003080:Cold Temperature; D003326:Coronary Circulation / Q000187:drug effects; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006334:Heart Function Tests; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009211:Myoglobin / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015424:Reperfusion; D012457:Salicylamides / Q000737:chemistry / Q000494:pharmacology*
C512608:3-nitro-N-methyl-salicylamide; D009211:Myoglobin; D017382:Reactive Oxygen Species; D012457:Salicylamides; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; C031060:salicylamide