title: Enhanced culture of Borrelia garinii and Borrelia afzelii strains on a solid BSK-based medium in anaerobic conditions.
abstract: The growth of 29 human strains from the three main pathogenic species of Borrelia burgdorferi sensu lato on a solid BSK-based medium was compared in two culture atmospheres: 3% CO(2) air and anaerobiosis. All strains grew under anaerobic conditions, whereas only 13 strains were able to grow in aerobiosis with 3% CO(2) (P<0.001). In the latter condition, 75% of the B. burgdorferi sensu stricto strains grew versus 33% of the B. garinii and B. afzelii strains. These data suggest that, especially for B. garinii and B. afzelii species, anaerobic conditions enhance growth yield and speed of low-passage Borrelia strains.
authors.affiliation: Laboratoire de Physiopathologie et d'Antibiologie Microbiennes, EA 3432, Laboratoire de Bactériologie de la Faculté de Médecine et Hopitaux Universitaires de Strasbourg, 3 rue Koeberlé, 67000 Strasbourg, France.
authors.forename: Sylvie Josiane
authors.initials: SJ
authors.lastname: De Martino
authors.forename: Christelle
authors.initials: C
authors.lastname: Sordet
authors.forename: Yves
authors.initials: Y
authors.lastname: Piémont
authors.forename: Eva
authors.initials: E
authors.lastname: Ruzic-Sabljic
authors.forename: Marie
authors.initials: M
authors.lastname: Thaddée Vetter
authors.forename: Henri
authors.initials: H
authors.lastname: Monteil
authors.forename: Jean
authors.initials: J
authors.lastname: Sibilia
authors.forename: Benoît
authors.initials: B
authors.lastname: Jaulhac
mesh_terms: D000693:Anaerobiosis; D001431:Bacteriological Techniques; D015748:Borrelia burgdorferi Group / Q000254:growth & development*; D018929:Cell Culture Techniques; D003470:Culture Media; D050296:Microbial Viability
chemical_list: D003470:Culture Media