title: Neovaginal mucosa after Vecchietti's laparoscopic operation for Rokitansky syndrome: structural and ultrastructural study.
abstract: OBJECTIVE
  This study was undertaken to evaluate structural and ultrastructural characteristics of the mucosa of neovaginae created by Vecchietti's laparoscopic operation for Rokitansky syndrome.
  
  
  METHODS
  Vaginoscopy and Schiller test were performed 3, 6, and 12 months after the operation in 106 patients. A biopsy specimen of the neovagina obtained 12 to 18 months after surgery in 19 patients was examined by light, scanning electron, and transmission electron microscopy.
  
  
  RESULTS
  At vaginoscopy, the neovaginal mucosa appeared smooth, lacking the folds that characterize the normal vagina; 12 months after the operation, an iodium-positive epithelium was present in all neovaginae. Mild ultrastructural modifications, as compared with normal vaginal mucosa, were reduced maturation, inflammatory infiltration, and tendency to superficial desquamation.
  
  
  CONCLUSIONS
  At a 12-month follow-up, the mucosa of neovaginae created by the Vecchietti technique is comparable to the normal vaginal mucosa, with mild structural and ultrastructural modifications that we believe might be due to reduced vascularization.
authors.affiliation: Dipartimento di Ostetricia, Ginecologia e Neonatologia, Fondazione Policlinico-Mangiagalli-Regina Elena, Università di Milano, Milan, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Fedele
authors.forename: Stefano
authors.initials: S
authors.lastname: Bianchi
authors.forename: Nicola
authors.initials: N
authors.lastname: Berlanda
authors.forename: Eleonora
authors.initials: E
authors.lastname: Fontana
authors.forename: Ricciarda
authors.initials: R
authors.lastname: Raffaelli
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bulfoni
authors.forename: Paola
authors.initials: P
authors.lastname: Braidotti
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D013509:Gynecologic Surgical Procedures; D006801:Humans; D010535:Laparoscopy; D009092:Mucous Membrane / Q000648:ultrastructure; D019738:Surgically-Created Structures; D013577:Syndrome; D014599:Uterus / Q000002:abnormalities*; D014621:Vagina / Q000002:abnormalities* / Q000601:surgery* / Q000648:ultrastructure