In vitro biocompatibility of a novel membrane of the composite poly(vinylidene-trifluoroethylene)/barium titanate.
This study was aimed at investigating the in vitro biocompatibility of a novel membrane of the composite poly(vinylidene-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT). Osteoblastic cells were obtained from human alveolar bone fragments and cultured under standard osteogenic condition until subconfluence. First passaged cells were cultured on P(VDF-TrFE)/BT and expanded polytetrafluoroethylene (e-PTFE--control) membranes in 24-well plates. Cell adhesion and spreading were evaluated at 30 min, and 4 and 24 h. For proliferation assay, cells were cultured for 1, 7, and 10 days. Cell viability was detected by trypan blue at 7 and 10 days. Total protein content and alkaline phosphatase (ALP) activity were measured at 7, 14, and 21 days. Cultures were stained with Alizarin red at 21 days, for detection of mineralized matrix. Data were compared by ANOVA and Student t test. Cell attachment (p = 0.001), cell number (p = 0.001), and ALP activity (p = 0.0001) were greater on P(VDF-TrFE)/BT. Additionally, doubling time was greater on P(VDF-TrFE)/BT (p = 0.03), indicating a decreased proliferation rate. Bone-like nodule formation took place only on P(VDF-TrFE)/BT. The present results showed that both membranes are biocompatible. However, P(VDF-TrFE)/BT presented a better in vitro biocompatibility and allowed bone-like nodule formation. Therefore, P(VDF-TrFE)/BT could be an alternative membrane to be used in guided tissue regeneration.
Beloti
Márcio M
MM
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, SP, Brazil.
de Oliveira
Paulo T
PT
Gimenes
Rossano
R
Zaghete
Maria A
MA
Bertolini
Márcio J
MJ
Rosa
Adalberto L
AL
D000469:Alkaline Phosphatase / Q000378:metabolism; D000880:Anthraquinones / Q000494:pharmacology; D017609:Barium Compounds / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000378:metabolism; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism; D011145:Polyvinyls / Q000737:chemistry*; D012038:Regeneration; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
D000880:Anthraquinones; D017609:Barium Compounds; D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D011145:Polyvinyls; C024547:barium titanate(IV); C073666:vinylidene fluoride-trifluoroethylene copolymer; C010078:alizarin; D014025:Titanium; D000469:Alkaline Phosphatase