[Effect of exogenous plasmid R68.45 on productive and lisogenic development of temperate bacteriophage ZF40 Erwinia carotovora].
The Erwinia carotoerora strains bearing exogenous plasmid R68.45 have been obtained by means of transconjugation. Dynamics and frequency characteristics of plasmid transfer to erwinia cells have been studied. Plasmid-phage interrelations have been studied for the first time in E. carotovora. It has been established that the presence of exogenous plasmid R68.45 affects essentially the culture growth parameters and lisogenization of E. carotovora by the wild phage ZF40 and its clear-mutants. The phage infection leads to the exclusion of plasmid R68.45 with different frequency (from 23 to 45% depending on the phage mutant). The presence of plasmid R68.45 in the erwinia cells destabilizes the defective lysogeny in E. carotovora.
Chervatiuk
N V
NV
Tovkach
F I
FI
D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D008242:Lysogeny; D016973:Pectobacterium carotovorum / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids / Q000235:genetics*; D014161:Transduction, Genetic