title: Magnetic core-shell Fe3O4@Ag nanoparticles coated carbon paste interface for studies of carcinoembryonic antigen in clinical immunoassay.
abstract: This study demonstrates a novel approach toward development of advanced immunosensors based on chemically functionalized core-shell Fe3O4@Ag magnetic nanoparticles, and the preparation, characterization, and measurement of relevant properties of the immunosensor useful for the detection of carcinoembryonic antigen (CEA) in clinical immunoassay. The immunosensor based on the combination of a magnetic nanocore and an Ag metallic shell shows good adsorption properties for the attachment of the CEA antibody selective to CEA. The core-shell nanostructure presents good magnetic properties to facilitate and modulate the way it was integrated into a carbon paste. Under optimal conditions, the resulting composite presents good electrochemical response for the detection of CEA, and allows detection of CEA at a concentration as low as 0.5 ng.mL(-1). Importantly, the proposed methodology could be extended to the detection of other antigens or biocompounds.
authors.affiliation: Key Laboratory of Analytical Chemistry (Chongqing), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400700, PR China.
authors.forename: Dianping
authors.initials: D
authors.lastname: Tang
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000097:blood*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002272:Carcinoembryonic Antigen; D005290:Ferric Compounds; C000499:ferric oxide; D012834:Silver; D002244:Carbon