Nanopillar sheets as a new type of cell culture dish: detailed study of HeLa cells cultured on nanopillar sheets.
Nanopillar sheets were fabricated with high-aspect ratio structures with a diameter of 160-1,000 nm and a height of 1 mum by nanoimprinting. The suitability of nanopillar sheets as a new type of cell culture dish was examined by studying the behavior of HeLa cells cultured on the sheets using light microscopy, scanning electron microscopy, and fluorescence microscopy observing actin and vinculin molecules. The nanopillar structure enabled easy subculture of the cells from the sheets without conventional trypsinization. Moreover, the HeLa cells divided and proliferated on the sheets in a different way to that found on petri dish because of the manner in which the cells adhered to the materials.
Nomura
Shinobu
S
Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, AIST, Tsukuba Central 6-13, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Kojima
Hiroko
H
Ohyabu
Yoshimi
Y
Kuwabara
Kosuke
K
Miyauchi
Akihiro
A
Uemura
Toshimasa
T
D018929:Cell Culture Techniques / Q000295:instrumentation*; D006367:HeLa Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D013499:Surface Properties