[Expression of immune response molecules and function of fas ligand on surface of AML WEHI-3 cells].
The purpose of this study was to investigate the expression of Fas, Fas ligand (FasL) and CD80 and function of FasL on the surface of acute myelomonocytic leukemia cells from WEHI-3 line. The expression of Fas, FasL and CD80 on the surface of WEHI-3 were detected by flow cytometry, the apoptosis of YAC-1 cell induced by FasL on the surface of WEHI-3 were detected by (3)H-TdR incorporation. The results showed that the expression rate of Fas, FasL and CD80 on the surface of WEHI-3 cells were (6.75 +/- 2.31)% (n = 5), (63.73 +/- 5.23)% (n = 5) and (5.06 +/- 0.41)% (n = 5) respectively. The apoptosis rate of YAC-1 cells (target cells) co-cultured with WEHI-3 cells (Effector cells) at the rate of 1:3, 1:10 and 1:30 were (26 +/- 4.5)%, (35 +/- 3.2)% and (43 +/- 2.7)% (n = 5) respectively. It is concluded that WEHI-3 cells have high expression of FasL and low expression of Fas and CD80 on their cell membrane, and can induce the apoptosis of Fas(+) YAC-1 cells.
Liu
Ling-Bo
LB
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. liulinbo@medmail.com.cn
Li
Wei-Ming
WM
He
Wei
W
Zou
Ping
P
D017209:Apoptosis / Q000502:physiology; D018122:B7-1 Antigen / Q000096:biosynthesis*; D002462:Cell Membrane / Q000378:metabolism; D053222:Fas Ligand Protein / Q000096:biosynthesis*; D006801:Humans; D015479:Leukemia, Myelomonocytic, Acute / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000096:biosynthesis*
D018122:B7-1 Antigen; D053222:Fas Ligand Protein; D019014:fas Receptor