title: Doxycycline decreases monocyte chemoattractant protein-1 in human lung epithelial cells.
abstract: Certain antibiotics possess anti-inflammatory properties and could potentially be used to treat inflammatory lung diseases associated with an influx of monocytes such as panbronchiolitis, asthma, cystic fibrosis, and bronchitis. Doxycycline is reported to possess anti-inflammatory effects. Monocyte chemoattractant protein-1 (MCP-1) is a major inflammatory cytokine and a powerful chemoattractant for monocytes. The authors hypothesized that doxycycline exerts its anti-inflammatory effects, in part, by reducing MCP-1 production. To test this hypothesis, A549 human lung epithelial cells were stimulated with cytomix in the presence or absence of doxycycline. In stimulated cells doxycycline decreased MCP-1 production by 95% and in monocyte chemotaxis assays migration decreased by 55%. However, doxycycline did decrease expression of MCP-1 mRNA and did not effect its stability. These data demonstrate that doxycycline modulates MCP-1 production and suggest that doxycycline may provide a new anti-inflammatory therapy for chronic lung diseases.
authors.affiliation: Research Service, Carl T. Hayden VA Medical Center, Phoenix, Arizona 85012, USA.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Raza
authors.forename: Janelle G
authors.initials: JG
authors.lastname: Ballering
authors.forename: John M
authors.initials: JM
authors.lastname: Hayden
authors.forename: Richard A
authors.initials: RA
authors.lastname: Robbins
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Hoyt
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000276:immunology; D004318:Doxycycline / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D011014:Pneumonia / Q000188:drug therapy* / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D012333:RNA, Messenger; D004318:Doxycycline