title: [Development of an immunochromayography assay method for the detection of Yersinia pestis].
abstract: OBJECTIVE
  To develop a method of immunochromatography assay (ICA) with sensitive, specific, rapid, simple and suitable for the detection of Yersinia pestis antigen at the local laboratories.
  
  
  METHODS
  Colloidal gold labeled with the anti-F1 antibody of Yersinia pestis, was connnected with the anti-F1 antibody of Yersinia pestis to pyroxylin membrane and assembled them to the dipstick of ICA.
  
  
  RESULTS
  Results showed that the rates of sensitivity for F1 antigen and Yersinia pestis were 1 ng/ml and 1.56 x 10(5) CFU/ml respectively. However, Yersinia pseudotuberculosis et al could not be detected by dipstick of ICA.
  
  
  CONCLUSIONS
  The method of ICA appeared to be consistent to those of r-IHA with better specificity and sensitivity but was simple and rapid for the detection of Yersinia pestis and F1 antigen.
authors.affiliation: Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Tan
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Qing
authors.initials: Q
authors.lastname: Duan
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods; D012680:Sensitivity and Specificity; D015010:Yersinia pestis / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; C086725:caf1 protein, Yersinia pestis