title: Hyperthermia-induced antitumor activity of thermosensitive polymer modified temperature-sensitive liposomes.
abstract: Temperature-sensitive liposomes (TS-liposomes) have been studied for chemotherapeutic purposes to enhance the release of anticancer drugs at tumor sites. In this study, we prepared poly(N-isopropylacrylamide-co-acrylamide) (PNIPAM-AAM) and polyethylene glycol (PEG)-modified TS-liposomes (PETS-liposomes). PETS-liposomes significantly increased in vitro drug release in serum compared with PEG-fixed or PNIPAM-AAM-modified liposomes. Furthermore, incorporation of both PNIPAM-AAM and PEG into PETS-liposomes enhanced the stabilities of liposomes in serum by inhibiting protein adsorption. In addition, to investigate the therapeutic efficacy of doxorubicin (DOX)-loaded PETS-liposomes, the in vivo antitumor activity of liposomes in combination with hyperthermia was evaluated in a B16F10 melanoma tumor-bearing mouse model. PETS-liposomes showed much higher levels of tumor growth inhibition than PEG-fixed or PNIPAM-AAM-modified TS-liposomes. Moreover, the antitumor activity of PETS-liposomes was enhanced significantly when they were administered in combination with hyperthermia. PETS-liposomes were found to be highly efficacious carriers for the in vivo delivery of anticancer drugs, and to have potential anticancer applications in combination with hyperthermia.
authors.affiliation: Medicinal Science Division, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, South Korea.
authors.forename: Hee Dong
authors.initials: HD
authors.lastname: Han
authors.forename: Min Soo
authors.initials: MS
authors.lastname: Choi
authors.forename: Taewon
authors.initials: T
authors.lastname: Hwang
authors.forename: Chung Kil
authors.initials: CK
authors.lastname: Song
authors.forename: Hasoo
authors.initials: H
authors.lastname: Seong
authors.forename: Tae Woo
authors.initials: TW
authors.lastname: Kim
authors.forename: Ho Suk
authors.initials: HS
authors.lastname: Choi
authors.forename: Byung Cheol
authors.initials: BC
authors.lastname: Shin
mesh_terms: D000180:Acrylic Resins; D000327:Adsorption; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D001798:Blood Proteins / Q000737:chemistry; D055598:Chemical Phenomena; D002626:Chemistry, Pharmaceutical; D002627:Chemistry, Physical; D004337:Drug Carriers; D005079:Excipients; D005334:Fever / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry*; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D011092:Polyethylene Glycols; D011108:Polymers; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D000970:Antineoplastic Agents; D001798:Blood Proteins; D004337:Drug Carriers; D005079:Excipients; D008081:Liposomes; D011108:Polymers; C052970:poly-N-isopropylacrylamide; D011092:Polyethylene Glycols