title: Genomic approaches for identifying DNA damage response pathways in S. cerevisiae.
abstract: DNA damage response pathways have been studied extensively in the budding yeast Saccharomyces cerevisiae, yet new genes with roles in the DNA damage response are still being identified. In this chapter we describe the use of functional genomic approaches in the identification of DNA damage response genes and pathways. These techniques take advantage of the S. cerevisiae gene deletion mutant collection, either as an ordered array or as a pool, and can be automated for high throughput.
authors.affiliation: Biochemistry, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Chang
authors.forename: Ainslie B
authors.initials: AB
authors.lastname: Parsons
authors.forename: Bilal H
authors.initials: BH
authors.lastname: Sheikh
authors.forename: Charles
authors.initials: C
authors.lastname: Boone
authors.forename: Grant W
authors.initials: GW
authors.lastname: Brown
mesh_terms: D001483:Base Sequence; D004249:DNA Damage; D017931:DNA Primers; D017353:Gene Deletion; D017930:Genes, Reporter; D016681:Genome, Fungal; D009154:Mutation; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D017931:DNA Primers