Update and challenges on proteomics in rice.
Rice is not only an important agricultural resource but also a model plant for biological research. Our previous review highlighted different aspects of the construction of rice proteome database, cataloguing rice proteins of different tissues and organelle, differential proteomics using 2-DE and functional characterization of some of the proteins identified (Komatsu, S., Tanaka, N., Proteomics 2005, 5, 938-949). In this review, the powerfulness and weaknesses of proteomic technologies as a whole and limitations of the currently used techniques in rice proteomics are discussed. The information obtained from these techniques regarding proteins modification, protein-protein interaction and the development of new methods for differential proteomics will aid in deciphering more precisely the functions of known and/or unknown proteins in rice.
Komatsu
Setsuko
S
Department of Molecular Genetics, National Institute of Agrobiological Sciences, Tsukuba, Japan. skomatsu@affrc.go.jp
Yano
Hiroyuki
H
D016000:Cluster Analysis; D012275:Oryza; D010750:Phosphoproteins / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D040901:Proteomics / Q000639:trends*; D015398:Signal Transduction
D010750:Phosphoproteins; D010940:Plant Proteins