title: Enhanced antifungal effect of the selective medium for the detection of Legionella species by a combination of cycloheximide, amphotericin B and thiabendazole.
abstract: The accurate detection of Legionella from environmental water samples using conventional plate culture methods is often made difficult by the overgrowth of non-target microorganisms on the selective agar plates. Acid pretreatment is a very effective pretreatment to decrease the overgrowth of heterotrophic bacteria. However, acid pretreatment would not be expected to eliminate molds. We evaluated the effects of four kinds of antifungal agents, individually and in combination, on the growth of Legionella strains and molds. Consequently, it was demonstrated that the combination of cycloheximide, amphotericin B and thiabendazole was very effective in eliminating molds on agar plates, and resulted in the improved detection of Legionella. Examination of 214 cooling tower water samples using the enhanced antifungal selective medium (CAT alpha) instead of GVPC alpha selective medium demonstrated a decrease in contamination by molds from 13.6% to 1.9% without affecting the growth of Legionella.
authors.affiliation: Tsukuba Research Laboratories, Aquas Corporation, 4-4 Midorigahara, Tsukuba, Ibaraki 300-2646, Japan. h_inoue0417@aquas.co.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Inoue
authors.forename: Akiko
authors.initials: A
authors.lastname: Noda
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takama
authors.forename: Tomoki
authors.initials: T
authors.lastname: Ishima
authors.forename: Kunio
authors.initials: K
authors.lastname: Agata
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D003470:Culture Media / Q000592:standards; D003513:Cycloheximide / Q000494:pharmacology; D004357:Drug Synergism; D007875:Legionella / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D013827:Thiabendazole / Q000494:pharmacology; D014871:Water Microbiology / Q000592:standards*
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D000666:Amphotericin B; D003513:Cycloheximide; D013827:Thiabendazole