title: Regioselective esterase-catalyzed feruloylation of L-arabinobiose.
abstract: The regioselective chemoenzymatic synthesis of O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1-->5)-L-arabinofuranose has been achieved. The reaction parameters affecting the feruloylation rate and conversion of the enzymatic synthesis, such as the composition of the reaction medium, substrate and enzyme concentration, have been investigated.
authors.affiliation: Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, GR-15700 Athens, Greece.
authors.forename: Christina
authors.initials: C
authors.lastname: Vafiadi
authors.forename: Evangelos
authors.initials: E
authors.lastname: Topakas
authors.forename: Paul
authors.initials: P
authors.lastname: Christakopoulos
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D002855:Chromatography, Thin Layer; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods; D008958:Models, Molecular; D015394:Molecular Structure; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D003373:Coumaric Acids; D004187:Disaccharides; C434937:arabinobiose; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase