Effect of smear layer and chlorhexidine treatment on the adhesion of Enterococcus faecalis to bovine dentin.
The aim of this in vitro study was to determine the effects of a smear layer and chlorhexidine (CHX) treatment on the adhesion of Enterococcus faecalis to bovine dentin. Forty dentin blocks from bovine incisors were prepared and randomly divided into four groups of 10 each. The blocks in group 1 were placed in sterile saline for 5 minutes, while those in group 2 were treated with 17% EDTA for 5 minutes. The blocks in group 3 were placed in 2% CHX for 7 days. The blocks in group 4 were treated with 17% EDTA for 5 minutes, and then placed in 2% CHX for 7 days. All the blocks were immersed in a suspension of E. faecalis for 3 hours. The bacteria adhering to the dentin surface were counted by examination using a scanning electron microscope. The most significant amount of bacteria was retained on the samples from group 1 (p < 0.05) and the smallest amount of bacteria adhered to the samples from group 4. These results suggest that a smear layer enhances the adherence of E. faecalis to the dentin, and CHX is effective in reducing the adherence of microorganisms.
Yang
Seung-Eun
SE
Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seodaemun-gu, Seoul, Republic of Korea.
Cha
Jeong-Heon
JH
Kim
Eui-Seong
ES
Kum
Kee-Yeon
KY
Lee
Chang-Young
CY
Jung
Il-Young
IY
D000704:Analysis of Variance; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D002417:Cattle; D002710:Chlorhexidine / Q000494:pharmacology*; D003804:Dentin / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D012388:Root Canal Irrigants / Q000494:pharmacology*; D016369:Smear Layer; D018709:Statistics, Nonparametric
D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants; D002710:Chlorhexidine