title: Intermittent pressure augmentation during retrograde cerebral perfusion under moderate hypothermia provides adequate neuroprotection: an experimental study.
abstract: OBJECTIVE
  For cerebral protection during aortic surgery, we introduced a novel retrograde cerebral perfusion method with intermittent pressure augmentation. We then assessed whether this novel method provides benefits similar to those provided by antegrade selective cerebral perfusion.
  
  
  METHODS
  Eighteen dogs were randomly divided into 3 groups: the RCP-INT group, intermittent-retrograde cerebral perfusion at 15 mm Hg with intermittent pressure augmentation to 45 mm Hg (n = 6); the ASCP group, antegrade selective cerebral perfusion at a flow rate of 10 mL x kg(-1) x min(-1) (n = 6); and the sham group, no circulatory arrest (n = 6). Cooling (26 degrees C) with cardiopulmonary bypass and 60 minutes of circulatory arrest were performed in the RCP-INT and ASCP groups. The levels of tau protein in the cerebrospinal fluid and the diameters of the retinal vessels were measured. The neurologic deficit scores and the histopathologic damage scores of the brains were determined.
  
  
  RESULTS
  The total postoperative tau protein levels (calculated as the area under the curve) did not differ significantly between the RCP-INT and ASCP groups (203 +/- 87 pg x mL(-1) x h vs 154 +/- 69 pg x mL(-1) x h, P = .95). The retinal vessels were effectively dilated at an augmented pressure of 45 mm Hg in the RCP-INT group. The total neurologic deficit score (0 = normal, 500 = brain death) and histopathologic damage score (0 = normal, 40 = worst) were not significantly different between the RCP-INT and ASCP groups (neurologic deficit score: 75 +/- 21 vs 70 +/- 21, P = .98; histopathologic damage score: 13.5 +/- 1.5 vs 14.2 +/- 1.3, P = .84).
  
  
  CONCLUSIONS
  Intermittent augmented pressure dilated the cerebral vessels, allowing adequate blood supply without injuring the brain. This retrograde cerebral perfusion method provides adequate neuroprotection during moderate hypothermia.
authors.affiliation: Department of Cardiothoracic Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan. mkawata-ths@umin.ac.jp
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kawata
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kitahori
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsukihara
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Morota
authors.forename: Minoru
authors.initials: M
authors.lastname: Ono
authors.forename: Noboru
authors.initials: N
authors.lastname: Motomura
authors.forename: Arata
authors.initials: A
authors.lastname: Murakami
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Suematsu
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001921:Brain / Q000098:blood supply*; D002545:Brain Ischemia / Q000517:prevention & control*; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D004285:Dogs; D007036:Hypothermia, Induced; D023421:Models, Animal; D009460:Neurologic Examination; D009474:Neurons / Q000473:pathology; D010477:Perfusion / Q000379:methods*; D011312:Pressure; D012119:Respiration; D012161:Retinal Artery; D012169:Retinal Vein; D016875:tau Proteins / Q000134:cerebrospinal fluid
chemical_list: D016875:tau Proteins