title: [Quantitative assessment of mRNA TGF-beta1 in liver tissue in connection with serum mean daily level of TGF-1 in chronic hepatitis B patient].
abstract: Monitoring of fibrosis process with the use of histopathologic studies on liver's bioptates is limited, so it is attempted to find reliable, obtained with less invasive methods, sensitive and reflecting fibrosis dynamics markers of this process. The aim of the study was simultaneously to assess liver's expression as well as circadian and mean daily TGF-betal concentration in serum of patients with chronic hepatitis type B in comparison to control group. Studies were performed on 50 patients (9 women, 41 men) aged 45.9 +/- 2.3 years with chronic hepatitis type B. Control group consisted of 20 patients (mean age 38.6 +/- 3.7 years), in which so called minimal changes without fibrosis were observed in histophatologic bioptate of liver. Blood for studies was collected every 4 houres during the day. Serum concentration of TGF-betal was assessed with the use of ELISA method, and expression of mRNA TGF-betal in liver with QRT-PCR method. No significant difference between circadian as well as mean daily serum TGF-betal concentration beetwen control group and the group with chronic hepatitis type B was shown. Increased expression of mRNA, TGF-betal in bioptate of liver of patients with chronic hepatitis type B in comparison to control group was noted. In "minimal changes" control group presence of significant positive correlation between expression of mRNA TGF-beta1 in liver and concentration of this cytokine in serum was shown, in the group of patients with chronic hepatitis B this connection was not noted.
authors.affiliation: Zakład Patofizjologii Katedry Patofizjologii i Endokrynologii Slaskiej Akademii Medycznej w Zabrzu.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Marek
authors.forename: Dariusz
authors.initials: D
authors.lastname: Kajdaniuk
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
authors.forename: Ewa
authors.initials: E
authors.lastname: Janczewska-Kazek
authors.forename: Barbara
authors.initials: B
authors.lastname: Strzałka
authors.forename: Marek
authors.initials: M
authors.lastname: Beniowski
authors.forename: Beata
authors.initials: B
authors.lastname: Kos-Kudła
authors.forename: Joanna
authors.initials: J
authors.lastname: Kajdaniuk
authors.forename: Danuta
authors.initials: D
authors.lastname: Niedziołka
authors.forename: Zofia
authors.initials: Z
authors.lastname: Ostrowska
authors.forename: Halina
authors.initials: H
authors.lastname: Borgiel-Marek
authors.forename: Lucyna
authors.initials: L
authors.lastname: Siemińiska
authors.forename: Mariusz
authors.initials: M
authors.lastname: Nowak
authors.forename: Dorota
authors.initials: D
authors.lastname: Pakuła
authors.forename: Aleksander
authors.initials: A
authors.lastname: Gatnar
authors.forename: Roman
authors.initials: R
authors.lastname: Gnot
authors.forename: Przemysław
authors.initials: P
authors.lastname: Filipczyk
mesh_terms: D002940:Circadian Rhythm; D005260:Female; D019694:Hepatitis B, Chronic / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000097:blood / Q000378:metabolism*; D053773:Transforming Growth Factor beta1
chemical_list: D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1