A simple, rapid and effective method for total RNA extraction from Lentinula edodes.
A rapid, inexpensive and reliable method for total RNA extraction from fruiting bodies of Lentinula edodes containing large quantities of polysaccharides and secondary metabolites is described. An initial extraction step using saturated NaCl solution facilitates the separation of nucleic acids from contaminants and, after further extraction with organic solvents and precipitation with 2-propanol, total RNA of high purity and suitable for applications such as cDNA synthesis, RT-PCR and Northern blot hybridization was obtained. The procedure may also have wider applicability for total RNA extraction from the tissues of other mushrooms.
Li
Jun-Hui
JH
College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China.
Tang
Chuan-Hong
CH
Song
Chun-Yan
CY
Chen
Ming-Jie
MJ
Feng
Zhi-Yong
ZY
Pan
Ying-Jie
YJ
D015152:Blotting, Northern / Q000379:methods; D048690:Fruiting Bodies, Fungal / Q000737:chemistry* / Q000235:genetics; D012313:RNA / Q000302:isolation & purification*; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D020942:Shiitake Mushrooms / Q000737:chemistry* / Q000235:genetics
D012462:Saline Solution, Hypertonic; D012313:RNA