Functional assays for replication protein A (RPA).
Replication protein A (RPA) is a heterotrimeric, single-stranded DNA-binding protein. RPA is conserved in all eukaryotes and is essential for DNA replication, DNA repair, and recombination. RPA also plays a role in coordinating DNA metabolism and the cellular response to DNA damage. Assays have been established for many of these reactions. This chapter provides an overview of the methods used for analyzing RPA-DNA interactions, RPA-protein interactions, and functional activities of RPA. Methods are also discussed for visualizing RPA in the cell and analyzing the effects of RPA function on cell cycle progression in mammalian cells.
Binz
Sara K
SK
Department of Biochemistry, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, USA.
Dickson
Anne M
AM
Haring
Stuart J
SJ
Wold
Marc S
MS
D002846:Chromatography, Affinity; D004260:DNA Repair; D004261:DNA Replication; D004797:Enzyme-Linked Immunosorbent Assay; D005454:Fluorescence Polarization; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D051716:Replication Protein A / Q000302:isolation & purification / Q000502:physiology*; D020349:Surface Plasmon Resonance
D011994:Recombinant Proteins; D051716:Replication Protein A