title: Role of DNA replication and repair in thymineless death in Escherichia coli.
abstract: Inhibition of DNA replication with hydroxyurea during thymine starvation of Escherichia coli shows that active DNA synthesis is not required for thymineless death (TLD). Hydroxyurea experiments and thymine starvation of lexA3 and uvrA DNA repair mutants rule out unbalanced growth, the SOS response, and nucleotide excision repair as explanations for TLD.
authors.affiliation: Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, USA.
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Morganroth
authors.forename: Philip C
authors.initials: PC
authors.lastname: Hanawalt
mesh_terms: D004260:DNA Repair; D004261:DNA Replication; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D007700:Kinetics; D012329:RNA, Bacterial / Q000235:genetics; D013941:Thymine / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D012329:RNA, Bacterial; D013941:Thymine