title: Effects of bone protein extract on human mesenchymal stem cells proliferation and differentiation.
abstract: Since its osteoinductive capacity has been established, demineralized bone matrix is considered a suitable alternative to bone autograft in the healing of osseous defects. The mechanisms of bone formation induction are still not fully understood. In this study we assessed the effects of a dispersion of bovine bone extract COLLOSS (BPE) with regard to proliferation and differentiation of a human mesenchymal stem cell line overexpressing human telomerase reverse transcriptase (hMSC-TERT). Proliferation rate was determined by (3)H-thymidine incorporation. The differentiation of hMSC-TERT cells to osteoblastic cells was assessed by means of measuring alkaline phosphatase activity and collagen synthesis in vitro. Both undifferentiated and osteoblast-differentiated hMSC-TERT cells were investigated for response to BPE. The metabolic responses to BPE were compared to unstimulated cells and cells stimulated with bovine collagen (COL). Undifferentiated hMSC-TERT cells responded to BPE with increased proliferation and decreased alkaline phosphatase activity. Osteoblastic differentiated hMSC-TERT cells had a diminished proliferative response and an increased alkaline phosphatase activity and collagen synthesis. Our study demonstrated significant metabolic effects of BPE on hMSC-TERT cells, which were highly dependant on the differentiated state of the cells.
authors.affiliation: Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, DK-8000 Aarhus C, Denmark.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Woo
authors.forename: Haisheng
authors.initials: H
authors.lastname: Li
authors.forename: Anette
authors.initials: A
authors.lastname: Baatrup
authors.forename: Andrea
authors.initials: A
authors.lastname: Krause
authors.forename: Moustapha
authors.initials: M
authors.lastname: Kassem
authors.forename: Cody
authors.initials: C
authors.lastname: Bunger
authors.forename: Martin
authors.initials: M
authors.lastname: Lind
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D002457:Cell Extracts / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism
chemical_list: D002457:Cell Extracts; D003094:Collagen; D000469:Alkaline Phosphatase