Optimization of multienzyme production by two mixed strains in solid-state fermentation.
F(3) and F(4) strains of Aspergillus niger were screened from five strains of fungi to produce multienzyme preparations (containing cellulase, hemicellulase, glucoamylase, pectinase, and acidic proteinase) as dietary supplementation. Enzyme activities indicated that 1:4 (F(3) to F(4)) was the optimum mixture proportion, and 0.3% (W/W) was the preferable pitching rate. In bran mash containing 54.5% (W/W) water, F(3) and F(4) could produce the supplementation better when cultured 30 to 36 h at 30 degrees C. Monofactorial and orthogonal experiments were performed to optimize media. Results of the variance and range analysis showed that the optimum medium contained 80 g of bran, 20 g of cottonseed powder, 1 g of (NH(4))(2)SO(4), and 0.1 g of KH(2)PO(4). When F(3) and F(4 )strains were cultured in the optimum medium containing 54.5% (W/W) water, the activity of cellulase, hemicellulase, glucoamylase, pectinase, and acidic proteinase reached 996; 15,863; 13,378; 7,621; and 5,583 U/g, respectively.
Wang
Xiu-Juan
XJ
The Key Laboratory of Crop Biology of Shandong, College of Life Science, Shandong Agricultural University, Tai'an 271018, People's Republic of China. baijg@sdau.edu.cn
Bai
Ji-Gang
JG
Liang
Yun-Xiang
YX
D000821:Animal Feed; D001234:Aspergillus niger / Q000201:enzymology*; D001236:Aspergillus oryzae / Q000201:enzymology*; D003470:Culture Media; D004798:Enzymes / Q000096:biosynthesis*; D005285:Fermentation; D007218:Industrial Microbiology; D015999:Multivariate Analysis; D013045:Species Specificity; D014867:Water
D003470:Culture Media; D004798:Enzymes; D014867:Water