Chemical characterization and quantification of siderophores produced by marine and terrestrial aspergilli.
Ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. All test isolates produced siderophores as indicated by a positive reaction in the FeCl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. Further, the test isolates were compared for their siderophore production potential and chemical characteristics. Examination of the chemical nature of the siderophores revealed that all test isolates produced hydroxamate siderophores that were trihydroxamate hexadentates. Wide-spread occurrence of siderophores in marine isolates indicate their functional role in maintaining overall productivity of coastal waters. Among all test aspergilli, marine Aspergillus versicolor was found to be the largest siderophore producer (182.5 microg/mL desferrioxamine mesylate equivalent), least siderophore production was recorded in a marine strain of Aspergillus niger (3.5 microg/mL desferrioxamine mesylate equivalent).
Vala
Anjana K
AK
Department of Life Sciences, Bhavnagar University, Gujarat, India. bharti_dave@rediffmail.com
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BP
Dube
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D001230:Aspergillus / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000378:metabolism; D001234:Aspergillus niger / Q000378:metabolism; D001235:Aspergillus ochraceus / Q000378:metabolism; D002264:Carboxylic Acids / Q000737:chemistry; D003676:Deferoxamine / Q000737:chemistry; D006877:Hydroxamic Acids / Q000737:chemistry; D058085:Iron Compounds / Q000737:chemistry; D012623:Seawater / Q000382:microbiology; D017262:Siderophores / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D012988:Soil Microbiology; D013045:Species Specificity; D014871:Water Microbiology
D002264:Carboxylic Acids; D006877:Hydroxamic Acids; D058085:Iron Compounds; D017262:Siderophores; D003676:Deferoxamine