title: Complete Sequence and Enhancer Function of the Homologous DNA Regions of Autographa californica Nuclear Polyhedrosis Virus.
abstract: The nucleotide sequence of the five regions of homologous DNA in the genome of Autographa californica nuclear polyhedrosis virus DNA was determined. The homology of repeated sequences within a region was 65 to 87%, and the consensus sequences for each region were 88% homologous to each other. Sequences proximal to the EcoRI sites were most conserved, while the distal sequences were least conserved. The EcoRI sites formed the core of a 28-base-pair imperfect inverted repeat. All homologous regions functioned as enhancers in a transient expression assay. A single EcoRI minifragment located between EcoRI-Q and -L enhanced the expression of 39CAT as efficiently as the regions containing numerous EcoRI repeats did.
authors.affiliation: Department of Entomology, Texas A&M University, and Texas Agricultural Experiment Station, College Station, Texas 77843-2475.
authors.forename: L A
authors.initials: LA
authors.lastname: Guarino
authors.forename: M A
authors.initials: MA
authors.lastname: Gonzalez
authors.forename: M D
authors.initials: MD
authors.lastname: Summers