The influence of various storage conditions on cell viability in amniotic membrane.
Up to now freeze-dried, gamma-sterilised or glycerol-preserved amniotic membranes (AMs) have widely been used in the field of ophthalmology and wound care (e.g. leg ulcers, burns). After some preservation processes in use, like freeze-drying or glycerol-preserving, the cells in the AM are no longer viable. Within this study we evaluated the influence of different short-term and long-term storage conditions on cell viability in AM. Therefore AMs from cesarean section placentae were washed and biopsied to evaluate the microbiological status and to determine the viability of the tissue. Additionally, viability under various storage conditions was examined by assessment of mitochondrial activity. Preservation included temperatures above and below 0 degrees C as well as various media compositions. As expected, cell viability in amnion decreases during storage, in fact the effect was more pronounced when stored frozen, but the higher viability of amnion obtained by storage above 0 degrees C with medium is associated with the limitation to a short period of storage of about 28 days. The evaluated preservation methods are the basis for future non-clinical in-vivo studies in which the possible benefit of amnion as a viable biomaterial in wound healing will be investigated.
Hennerbichler
Simone
S
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Linz-Vienna, Austria. simone.hennerbichler@blutz.o.redcross.or.at
Reichl
Bernd
B
Pleiner
Daniela
D
Gabriel
Christian
C
Eibl
Johann
J
Redl
Heinz
H
D000650:Amnion / Q000166:cytology*; D001419:Bacteria / Q000302:isolation & purification; D001706:Biopsy; D002470:Cell Survival; D003470:Culture Media; D005260:Female; D005615:Freezing; D006801:Humans; D009584:Nitrogen; D011247:Pregnancy; D011309:Preservation, Biological / Q000379:methods*; D013194:Staining and Labeling; D014343:Trypan Blue / Q000378:metabolism
D003470:Culture Media; D014343:Trypan Blue; D009584:Nitrogen