[Study on the association between transforming growth factor alpha gene TaqI variant and cleft lip with or without cleft palate].
OBJECTIVE
To study the association between transforming growth factor alpha gene (TGFalpha) TaqI variant and nonsyndromic cleft lip with or without cleft palate (nsCL/P) in Chinese population.


METHODS
TGFalpha TaqI variant was detected using polymerase chain reaction-restriction fragment length polymorphism for DNA samples of the 149 triads with nsCL/P affected child. We performed the Transmission/disequilibrium test and the family-based association study (FBAT) to identify the associations between this variant and risk of nsCL/P.


RESULTS
Significant distortion of C2 allele at TGFalpha TaqI locus in nsCL/P groups (P > 0.05) was not found. In the family-based association test, C2 allele and offspring C2C1 genotype was not found to be significantly associated with an increase risk of nsCL/P (P > 0.05).


CONCLUSIONS
Our findings did not suggest an association between offspring TGFalpha TaqI variant and the increased risk of nsCL/P in Chinese population.
Zhu
Jiang-hui
JH
Institute of Reproductive and Child Health, National Reference Laboratory on Reproductive Health Research Ministry of Health, Peking University, Beijing 100083, China.
Ren
Ai-guo
AG
Hao
Ling
L
Pei
Li-jun
LJ
Zhang
Bo-lan
BL
Zhou
Min-xia
MX
Sun
Xia-mei
XM
Jiang
Mei-fang
MF
Chen
Hai-lan
HL
Li
Zhu
Z
D002648:Child; D002681:China; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012306:Risk; D016211:Transforming Growth Factor alpha / Q000235:genetics*
D016211:Transforming Growth Factor alpha