Surface plasmon resonance biosensor for direct detection of antibody against Epstein-Barr virus.
This paper describes the direct label-free detection of antibodies against the Epstein-Barr virus (anti-EBNA) using a surface plasmon resonance (SPR) biosensor. The antibody detection was performed using the immunoreaction between anti-EBNA and a respective synthetic peptide (EBNA-1), which was conjugated with bovine serum albumin (BSA-EBNA) and immobilized on the sensor surface. Three immobilization chemistries for the attachment of BSA-EBNA were investigated to optimize ligand density and minimize loss of EBNA-1 immunoreactivity. The developed SPR biosensor functionalized with the optimal immobilization method was calibrated and characterized in terms of detection limit, reproducibility, regenerability and storability. It was demonstrated that the sensor is capable of detecting concentrations of anti-EBNA as low as 0.2 ng/ml (approximately 1 pM) both in buffer and 1% human serum and can be stored and regenerated for repeated use.
Vaisocherová
Hana
H
Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, 182 51 Prague, Czech Republic.
Mrkvová
Katerina
K
Piliarik
Marek
M
Jinoch
Pavel
P
Steinbachová
Marie
M
Homola
Jirí
J
D000906:Antibodies / Q000032:analysis* / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D019309:Epstein-Barr Virus Nuclear Antigens / Q000032:analysis* / Q000276:immunology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004854:Herpesvirus 4, Human / Q000276:immunology*; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods
D000906:Antibodies; D019309:Epstein-Barr Virus Nuclear Antigens; C417029:EBV-encoded nuclear antigen 1