LC-MS/MS screen for penitrem A and roquefortine C in serum and urine samples.
A rapid LC-MS/MS method, using a triple quadrupole/linear ion trap mass spectrometer, was developed for determination of penitrem A and roquefortine C in serum and urine samples. Penitrem A and roquefortine C were extracted from samples with methylene chloride. The extracts were injected onto a liquid chromatograph coupled with a hybrid triple quadrupole/linear ion trap mass spectrometer. Seven replicate fortifications of serum at 0.001 microg/g (1 ppb) each of penitrem A and roquefortine C gave average recoveries of 90% with 10% CV (relative standard deviation) and 97% with 3% CV, respectively. Seven replicate fortifications of urine at 0.001 microg/g (1 ppb) each of penitrem A and roquefortine C gave average recoveries of 98% with 12% CV and 100% with 6% CV, respectively. This is the first report of a positive mass spectrometric identification and quantitation of both compounds in urine and serum samples from dog intoxication cases.
Tor
Elizabeth R
ER
California Animal Health and Food Safety Laboratory System, Toxicology Laboratory, University of California, Davis, California 95616, USA. ertor@ucdavis.edu
Puschner
Birgit
B
Filigenzi
Michael S
MS
Tiwary
Asheesh K
AK
Poppenga
Robert H
RH
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D006576:Heterocyclic Compounds, 4 or More Rings / Q000097:blood / Q000652:urine; D006801:Humans; D007211:Indoles / Q000097:blood* / Q000652:urine*; D013058:Mass Spectrometry / Q000379:methods*; D009183:Mycotoxins / Q000097:blood* / Q000652:urine*; D010879:Piperazines / Q000097:blood / Q000652:urine; D012015:Reference Standards
D006576:Heterocyclic Compounds, 4 or More Rings; D007211:Indoles; D009183:Mycotoxins; D010879:Piperazines; C003626:tremortin; C012536:roquefortine