ClcR-based biosensing system in the detection of cis-dihydroxylated (chloro-)biphenyls.
Polychlorinated biphenyls (PCBs) are a group of organic pollutants that are persistent when released into the environment. Among the metabolites of PCBs, dihydroxylated PCBs are also considered as toxic compounds. Various studies have shown that dihydroxylated PCBs affect the reproductive, immune, nervous, and endocrine systems. Detection of these chemicals in environmental and biological samples could provide first-hand information about their levels and lead to a better understanding of their role in toxicity. To that end, we developed a sensing system for the detection of dihydroxylated PCBs based on the clc operon. The Pseudomonas putida clc operon encodes a catabolic pathway for degradation of chlorocatechols, which are major metabolites of a large number of chlorinated compounds. In P. putida, the expression of these genes is regulated by a protein encoded by the gene clcR located upstream from the clcABD genes. We demonstrate here for the first time that dihydroxy PCBs can also induce the clc operon. Our sensing system employs P. putida bacteria harboring a plasmid in which the reporter gene, lacZ, is under the control of the regulatory protein ClcR. Consequently, when exposed to dihydroxy PCBs, the bacteria express beta-galactosidase in an amount related to the concentration of the corresponding dihydroxy PCB. Various dihydroxylated PCBs, differing in the number and position of chlorines and in the position of hydroxyls, were tested for their ability to induce expression of beta-galactosidase. Detection limits as low as 1 x 10(-6) mol L(-1) were obtained for various dihydroxylated PCBs.
Feliciano
Jessika
J
Department of Chemistry, University of Kentucky, Lexington, KY 40506-0055, USA.
Xu
Shifen
S
Guan
Xiyuan
X
Lehmler
Hans-Joachim
HJ
Bachas
Leonidas G
LG
Daunert
Sylvia
S
D015374:Biosensing Techniques / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006900:Hydroxylation; D015394:Molecular Structure; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000737:chemistry*; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*
D015534:Trans-Activators; D011078:Polychlorinated Biphenyls