Phosphoproteomic analysis of the human pituitary.
The pituitary is the central endocrine gland that regulates the functions of various target organs in the human body. Because of the pivotal regulatory role of the pituitary, it is essential to define on a global scale the components of the pituitary protein machinery, including a comprehensive characterization of the post-translational modifications of the pituitary proteins. Of particular interest is the examination of the phosphorylation status of the pituitary in health and disease. Towards the goal of global profiling of pituitary protein phosphorylation, we report here the application of the in-gel IEF-LC-MS/MS approach to the study of the pituitary phosphoproteome. The analytical strategy combined isoelectric focusing in immobilized pH gradient strips with immobilized metal ion affinity chromatography and mass spectrometry. With this method, a total of 50 phosphorylation sites were characterized in 26 proteins. Because the investigation involved primary tissue, the findings provide a direct glimpse into the phosphoprotein machinery operating within the human pituitary tissue microenvironment.
Beranova-Giorgianni
Sarka
S
Charles B. Stout Neuroscience Mass Spectrometry Laboratory, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Zhao
Yingxin
Y
Desiderio
Dominic M
DM
Giorgianni
Francesco
F
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D002853:Chromatography, Liquid; D002864:Chromogranins / Q000378:metabolism; D019004:Galanin / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
D002864:Chromogranins; D010750:Phosphoproteins; D019004:Galanin; D000324:Adrenocorticotropic Hormone; D013006:Growth Hormone