Detection of mycobacterial antigen responses in lung but not blood in HIV-tuberculosis co-infected subjects.
Forty-seven HIV-infected adults had broncho-alveolar lavage stimulated with purified protein derivate of Mycobacterium tuberculosis. Eighteen of 19 (95%) with tuberculosis co-infection had interferon-gamma synthetic CD4 lymphocyte responses > 1% versus three of 28 (11%) without (P < 0.0001). Lung response was unrelated to blood CD4 cell count. BAL HIV tuberculosis responses were similar in 25 HIV-uninfected tuberculosis patients. Responses in matched blood samples were often undetectable. Therefore, immunological tuberculosis assays seem less affected by HIV co-infection when lung-based.
Breen
Ronan A M
RA
Department of Immunology, Royal Free & University College Medical School, Royal Free Hospital, London, UK. r.breen@medsch.ucl.ac.uk
Janossy
George
G
Barry
Simon M
SM
Cropley
Ian
I
Johnson
Margaret A
MA
Lipman
Marc C I
MC
D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis* / Q000097:blood; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D018791:CD4 Lymphocyte Count; D015658:HIV Infections / Q000150:complications* / Q000276:immunology; D015497:HIV-1; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D008168:Lung / Q000276:immunology*; D008875:Middle Aged; D009169:Mycobacterium tuberculosis; D013601:T-Lymphocytes / Q000276:immunology; D014376:Tuberculosis / Q000150:complications* / Q000276:immunology
D000942:Antigens, Bacterial; D007371:Interferon-gamma