title: Dopamine receptors oppositely regulate cocaine-induced transcription factor CREB activation.
abstract: OBJECTIVE
  To study the role of dopamine receptors in the regulation of the activity of transcription factor cAMP response element-binding protein (CREB) after cocaine treatment.
  
  
  METHODS
  By using dopamine receptor antagonists SCH23390 and nafadotride, the activation of CREB by D1 and D3 dopamine receptors after cocaine treatment and role of extracellular signal-regulated kinase (ERK) in cocaine-induced CREB activation were examined by Western blotting, which was also employed for determination of the effect of SCH23390 and nafadotride on CREB activation.
  
  
  RESULTS
  D1 receptor antagonist could inhibit cocaine-induced CREB activation, while D3 receptor antagonist enhanced cocaine-induced CREB activation. Dopamine receptor antagonists SCH23390 and nafadotride did not induce CREB activation. SL327, a MEK inhibitor, inhibited cocaine-induced CREB activation.
  
  
  CONCLUSIONS
  D1 and D3 dopamine receptors can oppositely regulate CREB activation after cocaine treatment and this regulation depends on ERK signaling pathway.
authors.affiliation: Institute of Molecular Immunology, Southern Medical University, Guangzhou 510515, China. yunnuliu@fimmu.com
authors.forename: Nu-yun
authors.initials: NY
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiao-ning
authors.initials: XN
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D015153:Blotting, Western; D003042:Cocaine / Q000494:pharmacology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D018492:Dopamine Antagonists / Q000494:pharmacology; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051379:Mice; D009281:Naphthalenes / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology; D017447:Receptors, Dopamine D1 / Q000037:antagonists & inhibitors / Q000502:physiology*; D050637:Receptors, Dopamine D3 / Q000037:antagonists & inhibitors / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D001552:Benzazepines; D017362:Cyclic AMP Response Element-Binding Protein; D018492:Dopamine Antagonists; D018765:Dopamine Uptake Inhibitors; D009281:Naphthalenes; D011759:Pyrrolidines; D017447:Receptors, Dopamine D1; D050637:Receptors, Dopamine D3; D048049:Extracellular Signal-Regulated MAP Kinases; D003042:Cocaine; C097285:nafadotride