[Transplantation of autologous adult bone marrow stem cells in patients with severe peripheral arterial occlusion disease].
BACKGROUND
For many patients with severe peripheral arterial occlusion disease (PAOD) an interventional or surgical treatment is not feasible. The regenerative potential of adult autologous mononuclear stem cells could contribute to neoangiogenesis.


METHODS
Ten patients with severe PAOD were included. The walking distance was < 200 m and no interventional or surgical treatment was possible. After harvesting of 80 ml of bone marrow the mononuclear cell fraction was separated. Thereafter, intraarterial (10 ml into the common femoral artery) and intramuscular (5 ml into the muscles of the thigh and the lower leg) transplantation of the cell suspension was performed.


RESULTS
After 2 months the walking distance was enhanced significantly in all patients. Furthermore, a significant improvement of ankle-brachial index at rest, capillary-venous oxygen saturation and parameters of venous occlusion plethysmography was seen. No complications or side effects could be monitored.


CONCLUSIONS
These results demonstrate, that the combined intraarterial and intramuscular transplantation of autologous adult bone marrow stem cells leads to a significant improvement of perfusion indices in patients with severe PAOD.
Bartsch
Thomas
T
Klinik für Kardiologie, Pneumologie und Angiologie, Heinrich-Heine-Universität Düsseldorf.
Falke
Till
T
Brehm
Michael
M
Zeus
Tobias
T
Kögler
Gesine
G
Wernet
Peter
P
Strauer
Bodo-Eckehard
BE
D000368:Aged; D001157:Arterial Occlusive Diseases / Q000628:therapy*; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation / Q000502:physiology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007269:Injections, Intra-Arterial; D007273:Injections, Intramuscular; D007511:Ischemia / Q000628:therapy*; D007866:Leg / Q000098:blood supply*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010100:Oxygen / Q000097:blood; D016896:Treatment Outcome; D016138:Walking / Q000502:physiology
D010100:Oxygen