A batch chemiluminescence determination of enoxacin using a tris-(1,10-phenanthroline)ruthenium(II)-cerium(IV) system.
A batch type chemiluminescence (CL) determination of enoxacin is described. In this work, it was observed that enoxacin could enhance the chemiluminescence (CL) emission Ru(phen)3(2+)-Ce(IV) system and this enhancement effect was dependent on the concentration of enoxacin, based on which, CL system was established for the determination of enoxacin. Under the optimum experimental conditions, the linear range and detection limit are 0.6406-64.06 microg/ml and 0.0210 microg/ml, respectively. The R.S.D. is 1.75%. (n = 10). The proposed method has been applied to detect the content of enoxacin in pharmaceutical formulation and human serum with satisfactory results. The possible mechanism of the CL reaction was discussed.
Karim
Mohammad Mainul
MM
Department of Chemistry, Kyungpook National University, Taegu 702-701, South Korea.
Lee
Sang Hak
SH
Lee
Hyun Sook
HS
Bae
Zun Ung
ZU
Choi
Kyoung Hye
KH
D000890:Anti-Infective Agents / Q000032:analysis* / Q000737:chemistry; D002563:Cerium / Q000737:chemistry*; D015365:Enoxacin / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements; D015394:Molecular Structure; D010618:Phenanthrolines / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013464:Sulfuric Acids / Q000737:chemistry; D013607:Tablets
D000890:Anti-Infective Agents; D010618:Phenanthrolines; D013464:Sulfuric Acids; D013607:Tablets; D002563:Cerium; D015365:Enoxacin; D012428:Ruthenium; C033158:sulfuric acid