title: Antibodies Against the Genome-Linked Protein VPg of Cowpea Mosaic Virus Recognize a 60,000-Dalton Precursor Polypeptide.
abstract: We have prepared a rabbit antiserum specifically directed against the genome-linked protein (VPg) of cowpea mosaic virus by injecting an hydrolysate of purified virion RNA. Using this antiserum as a probe in combination with "Western" (protein) blots of subcellular fractions of cowpea mosaic virus-infected cowpea (Vigna unguiculata) cells, we have detected a bottom component RNA-encoded, 60,000-dalton polypeptide which is membrane bound and presumably represents the immediate precursor of VPg.
authors.affiliation: Department of Molecular Biology, Agricultural University, 6703 BC Wageningen, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Zabel
authors.forename: M
authors.initials: M
authors.lastname: Moerman
authors.forename: F
authors.initials: F
authors.lastname: van Straaten
authors.forename: R
authors.initials: R
authors.lastname: Goldbach
authors.forename: A
authors.initials: A
authors.lastname: van Kammen