title: Investigation of free radical scavenging enzyme activities and lipid peroxidation in human placental tissues with miscarriage.
abstract: BACKGROUND
  Miscarriage (early pregnancy failure) is a pregnancy-related disease, the pathophysiology of which is still not completely understood. Lipid peroxidation and alterations in antioxidant enzyme activities may be of importance in the pathogenesis of this disorder. This study was planned to investigate the possible relation between free radical scavenging enzyme activities and lipid peroxidation levels in placenta tissues with miscarriage.
  
  
  METHODS
  Placental tissue samples were obtained from 21 patients who had miscarried and 25 normal pregnant women undergoing elective abortion as a control group. Total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) enzyme activities and levels of thiobarbituric acid reactive substances (TBARS), antioxidant potential (AOP), and nonenzymatic superoxide radical scavenger activity (NSSA) were measured in the placental tissues.
  
  
  RESULTS
  GSH-Px, CAT activities, and TBARS levels were found to be significantly increased, while T-SOD and NSSA values decreased in patients with early pregnancy failure when compared with women undergoing elective abortion (control group). However, there were no significant differences in AOP levels between the groups.
  
  
  CONCLUSIONS
  Our results reflect oxidative stress in placenta tissues of early pregnancy failure, as the oxidative processes seem to be counteracted by the physiologic activation of antioxidant enzymes such as CAT and GSH-Px. Moreover, a compensatory mechanism might be developed against possible oxidative damage in patients with miscarriage.
authors.forename: Aydan
authors.initials: A
authors.lastname: Biri
authors.forename: Mustafa
authors.initials: M
authors.lastname: Kavutcu
authors.forename: Nuray
authors.initials: N
authors.lastname: Bozkurt
authors.forename: Erdinç
authors.initials: E
authors.lastname: Devrim
authors.forename: Nilhan
authors.initials: N
authors.lastname: Nurlu
authors.forename: Iker
authors.initials: I
authors.lastname: Durak
mesh_terms: D000022:Abortion, Spontaneous / Q000201:enzymology / Q000378:metabolism; D000975:Antioxidants / Q000032:analysis; D002374:Catalase / Q000032:analysis; D004798:Enzymes / Q000032:analysis*; D005260:Female; D016166:Free Radical Scavengers / Q000032:analysis*; D005979:Glutathione Peroxidase / Q000032:analysis; D006801:Humans; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D010920:Placenta / Q000201:enzymology* / Q000378:metabolism*; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D000975:Antioxidants; D004798:Enzymes; D016166:Free Radical Scavengers; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase