Infrared micro-spectroscopic studies of epithelial cells.
We report results from a study of human and canine mucosal cells, investigated by infrared micro-spectroscopy, and analyzed by methods of multivariate statistics. We demonstrate that the infrared spectra of individual cells are sensitive to the stage of maturation, and that a distinction between healthy and diseased cells will be possible. Since this report is written for an audience not familiar with infrared micro-spectroscopy, a short introduction into this field is presented along with a summary of principal component analysis.
Romeo
Melissa
M
Department of Chemistry and Biochemistry, City University of New York, Hunter College, New York, NY 10021, USA. m.romeo@neu.edu
Mohlenhoff
Brian
B
Jennings
Michael
M
Diem
Max
M
D000818:Animals; D002584:Cervix Uteri / Q000166:cytology; D004285:Dogs; D004847:Epithelial Cells / Q000737:chemistry* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008853:Microscopy / Q000379:methods; D009061:Mouth Mucosa / Q000166:cytology; D013055:Spectrophotometry, Infrared