Tight junctions and cell-cell interactions.
Chronic inflammation in mucosal tissues can influence epithelial barrier function via pro-inflammatory cytokines such as interferon (IFN)-gamma and tumor necrosis factor-alpha. Increased mucosal levels of these cytokines have been observed in mucosal biopsies from patients with a chronic inflammatory condition referred to as inflammatory bowel disease. Paracellular permeability across epithelial cells is regulated by tight junctions (TJs), which are the apical most junctions in epithelial cells. Given that pro-inflammatory cytokines modulate the epithelial barrier and that TJs regulate epithelial permeability, we analyzed the influence of IFN-gamma on U function/structure. Our results suggest that IFN-gamma induced a time-dependent increase in paracellular permeability that was associated with internalization of TJ transmembrane proteins, occludin, junction adhesion molecule A, and claudin-1. In this chapter, we focus on selected methods used to investigate the influence of IFN-gamma on epithelial barrier function.
Utech
Markus
M
Department of Pathology and Laboratory Medicine, Epithelial Pathobiology Unit, Emory University School of Medicine, Atlanta, GA, USA.
Brüwer
Matthias
M
Nusrat
Asma
A
D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D017097:Electric Impedance; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D015212:Inflammatory Bowel Diseases / Q000378:metabolism / Q000473:pathology; D007371:Interferon-gamma / Q000378:metabolism* / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D010539:Permeability / Q000187:drug effects; D051381:Rats; D019108:Tight Junctions / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015815:Cell Adhesion Molecules; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma