title: Nucleotide mutations in purA gene and pur operon promoter discovered in guanosine- and inosine-producing Bacillus subtilis strains.
abstract: The promoter region of the pur operon, which contains 12 genes for inosine monophosphate biosynthesis from phosphoribosylpyrophosphate, and the purA gene, encoding the adenylosuccinate synthetase, were compared among wild-type and three purine-producing Bacillus subtilis strains. A single nucleotide deletion at position 55 (relative to translation start site) in purA gene was found in a high inosine-producing strain and in a high guanosine-producing strain, which correlates with the absence of adenylosuccinate synthetase activity in these strains. Within the pur operon promoter of high guanosine-producing strain, in addition to a single nucleotide deletion in PurBox1 and a single nucleotide substitution in PurBox2, there were 4 substitutions in the flanking region of the PurBoxes and 32 nucleotide mutations in the 5' untranslated region. These mutations may explain the purine accumulation in purine-producing strains and be helpful to the rational design of high-yield recombinant strains.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai, PR China. jiangchaoqian@ecust.edu.cn
authors.forename: Jiangchao
authors.initials: J
authors.lastname: Qian
authors.forename: Xianpeng
authors.initials: X
authors.lastname: Cai
authors.forename: Ju
authors.initials: J
authors.lastname: Chu
authors.forename: Yingping
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Siliang
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000265:Adenylosuccinate Synthase / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D006151:Guanosine / Q000378:metabolism*; D007288:Inosine / Q000378:metabolism*; D007291:Inosine Monophosphate / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D009711:Nucleotides / Q000235:genetics; D009876:Operon / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011687:Purines / Q000378:metabolism
chemical_list: D009711:Nucleotides; D011687:Purines; D006151:Guanosine; D007291:Inosine Monophosphate; D007288:Inosine; D000265:Adenylosuccinate Synthase; C030985:purine