title: Aspm specifically maintains symmetric proliferative divisions of neuroepithelial cells.
abstract: The ASPM (abnormal spindle-like microcephaly-associated) protein has previously been implicated in the determination of human cerebral cortical size, but the cell biological basis of this regulation has not been studied. Here we investigate the role of Aspm in mouse embryonic neuroepithelial (NE) cells, the primary stem and progenitor cells of the mammalian brain. Aspm was found to be concentrated at mitotic spindle poles of NE cells and to be down-regulated with their switch from proliferative to neurogenic divisions. Upon RNA interference in telencephalic NE cells, Aspm mRNA is reduced, mitotic spindle poles lack Aspm protein, and the cleavage plane of NE cells is less frequently oriented perpendicular to the ventricular surface of the neuroepithelium. The alteration in the cleavage plane orientation of NE cells increases the probability that these highly polarized cells undergo asymmetric division, i.e., that apical plasma membrane is inherited by only one of the daughter cells. Concomitant with the resulting increase in abventricular cells in the ventricular zone, a larger proportion of NE cell progeny is found in the neuronal layer, implying a reduction in the number of NE progenitor cells upon Aspm knock-down relative to control. Our results demonstrate that Aspm is crucial for maintaining a cleavage plane orientation that allows symmetric, proliferative divisions of NE cells during brain development. These data provide a cell biological explanation of the primary microcephaly observed in humans with mutations in ASPM, which also has implications for the evolution of mammalian brains.
authors.affiliation: *Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany; and.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Fish
authors.affiliation: *Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany; and.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kosodo
authors.affiliation: Max Planck Institute of Evolutionary Anthropology, Deutscher Platz 6, D-04103 Leipzig, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Enard
authors.affiliation: Max Planck Institute of Evolutionary Anthropology, Deutscher Platz 6, D-04103 Leipzig, Germany.
authors.forename: Svante
authors.initials: S
authors.lastname: Pääbo
authors.affiliation: *Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany; and huttner@mpi-cbg.de.
authors.forename: Wieland B
authors.initials: WB
authors.lastname: Huttner
mesh_terms: D000818:Animals; D019556:COS Cells; D002148:Calmodulin-Binding Proteins; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D015536:Down-Regulation; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D046569:Neuroepithelial Cells / Q000166:cytology* / Q000378:metabolism*; D034622:RNA Interference; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: C512460:ASPM protein, mouse; D002148:Calmodulin-Binding Proteins; D009419:Nerve Tissue Proteins