title: Immunostimulatory oligodeoxynucleotide from Bifidobacterium longum suppresses Th2 immune responses in a murine model.
abstract: We have reported previously that novel immunostimulatory sequence (ISS) oligodeoxynucleotide (ODN) BL07S from a probiotic strain of Bifidobacterium longum inhibited immunoglobulin (Ig) E production in vitro. However, whether ISS-ODNs from probiotics regulate T helper type 2 (Th2)-polarized immune reactions in vivo remains unclear. To evaluate the inhibitory effects of ODN BL07S on type I allergic response, BALB/c mice were injected with or without ODN BL07S in the presence of ovalbumin (OVA) on days 0 and 14. Serum Ig levels (IgE, IgG1 and IgG2a) and cytokine levels (interferon (IFN)-gamma, interleukin (IL)-12, IL-4, IL-5, IL-10 and IL-13) were investigated in splenocyte cultures from days 14-28. Production of OVA-specific and total IgE were significantly suppressed by administration of ODN BL07S, but not by ODN BL06S, a non-ISS-ODN. Compared to controls, ODN BL07S induced significantly lower levels of Th2 cytokines (IL-4 and IL-5) in splenocyte cultures, and significantly higher levels of serum OVA-specific IgG2a. These effects of ODN BL07S on modulation of Th2 immune response were dose-dependent. The present results demonstrate that ODN BL07S from genomic DNA of B. longum BB536 prevents antigen-induced Th2 immune responses in vivo, suggesting that ISS-ODNs from probiotics might be useful in preventing allergic disease.
authors.affiliation: Food Research and Development Laboratory, Morinaga Milk Industry Co. Ltd, Zama, Japan. no_takah@morinagamilk.co.jp
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Kitazawa
authors.forename: N
authors.initials: N
authors.lastname: Iwabuchi
authors.forename: J Z
authors.initials: JZ
authors.lastname: Xiao
authors.forename: K
authors.initials: K
authors.lastname: Miyaji
authors.forename: K
authors.initials: K
authors.lastname: Iwatsuki
authors.forename: T
authors.initials: T
authors.lastname: Saito
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D001644:Bifidobacterium / Q000235:genetics*; D015415:Biomarkers / Q000097:blood; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007165:Immunosuppression Therapy; D015847:Interleukin-4 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D010047:Ovalbumin; D019936:Probiotics; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000628:therapy*; D013154:Spleen / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D015415:Biomarkers; D007074:Immunoglobulin G; D015848:Interleukin-5; D009838:Oligodeoxyribonucleotides; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin