Hepatocellular carcinoma model cell lines with two distinct migration modes.
Migration is an essential feature of metastatic cancer cells. To understand how motility is regulated in hepatocellular carcinoma, we analyzed gene expression profiles of mouse model cell lines we established from transgenic mice carrying SV40 large T antigen. A non-motile HC9 cell line was isolated from mouse liver tumors, and two additional cell lines, HCM1 and HCM4, were derived from HC9 cells. We found that both HCM1 and HCM4 cells were substantially more migratory than HC9, and that HCM1 generated tumor nodules in nude mice. In contrast to HCM4 cells that exhibited mesenchymal cell-type gene expression similar to HC9 cells, HCM1 cells appeared to have undergone a mesenchymal-amoeboidal transition. Thus, HCM1 and HCM4 cells have distinct migration and gene expression patterns, and together with HC9 cells, they can serve as model cell lines for understanding how migration is acquired and controlled in hepatocellular carcinoma.
Lee
In Jeong
IJ
Functional Genomics Research Center, Division of Molecular Therapeutics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, South Korea.
Li
Zhong Shu
ZS
Lee
Young Nam
YN
Jin
Xue-Jun
XJ
Lee
Jeong-Hyung
JH
Kim
Seon-Young
SY
Kim
Nam-Soon
NS
Lee
Dong Chul
DC
Lee
Heun-Sik
HS
Yang
Suk-Jin
SJ
Kim
Soo Jung
SJ
Yeom
Young Il
YI
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002460:Cell Line; D002465:Cell Movement; D020869:Gene Expression Profiling; D008113:Liver Neoplasms / Q000473:pathology*; D051379:Mice; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured