Two new bradykinin-related peptides from the venom of the social wasp Protopolybia exigua (Saussure).
Two bradykinin-related peptides (Protopolybiakinin-I and Protopolybiakinin-II) were isolated from the venom of the social wasp Protopolybia exigua by RP-HPLC, and sequenced by Edman degradation method. Peptide sequences of Protopolybiakinin-I and Protopolybiakinin-II were DKNKKPIRVGGRRPPGFTR-OH and DKNKKPIWMAGFPGFTPIR-OH, respectively. Synthetic peptides with identical sequences to the bradykinin-related peptides and their biological functions were characterized. Protopolybiakinin-I caused less potent constriction of the isolated rat ileum muscles than bradykinin (BK). In addition, it caused degranulation of mast cells which was seven times more potent than BK. This peptide causes algesic effects due to the direct activation of B(2)-receptors. Protopolybiakinin-II is not an agonist of rat ileum muscle and had no algesic effects. However, Protopolybiakinin-II was found to be 10 times more potent as a mast cell degranulator than BK. The amino acid sequence of Protopolybiakinin-I is the longest among the known wasp kinins.
Mendes
Maria Anita
MA
CEIS, Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University (UNESP), Rio Claro, SP 13506-900, Brazil.
Palma
Mario Sergio
MS
D000595:Amino Acid Sequence; D000818:Animals; D001920:Bradykinin / Q000737:chemistry* / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D008969:Molecular Sequence Data; D009619:Nociceptors / Q000187:drug effects; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology; D014862:Wasp Venoms / Q000737:chemistry* / Q000235:genetics; D014863:Wasps
D010455:Peptides; D014665:Vasodilator Agents; D014862:Wasp Venoms; C519177:protopolybiakinin-1, Protopolybia exigua; C519178:protopolybiakinin-2, Protopolybia exigua; D001920:Bradykinin