Characterization of Temperate Bacillus Bacteriophage phi105.
Temperate Bacillus phage phi105 is serologically unrelated to previously described virulent Bacillus phages. Phage phi105 is incapable of generalized transduction. Prophage phi105 is inducible with mitomycin C. Phage phi105 contains double-stranded deoxyribonucleic acid (DNA) with a molecular weight of about 25 x 10(7) as determined by band sedimentation and electron microscopy. The per cent guanine plus cytosine of phi105 DNA is 43.5 as determined by buoyant density in CsCl and by thermal denaturation. Phage phi105 DNA may contain complementary single-stranded ends.
Birdsell
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Department of Microbiology, Scripps Clinic and Research Foundation, La Jolla, California 92037.
Hathaway
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Rutberg
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