Cloning and characterization of a root-specific expressing gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from Ginkgo biloba.
3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC: 1.1.1.34) catalyzes the first committed step in mevalonic acid (MVA) pathway for biosynthesis of isoprenoids. The full-length cDNA encoding HMGR was isolated from Ginkgo biloba for the first time (designated as GbHMGR, GenBank accession number AY741133), which contained a 1713 bp ORF encoding 571 amino acids. The GbHMGR genomic DNA sequence was also obtained, revealing GbHMGR had four exons and three introns. The deduced GbHMGR protein showed high identity to other plant HMGRs and contained two trans-membrane domains and a catalytic domain. The three dimensional model of GbHMGR represented a typical spatial structure of HMGRs. The Southern blot and RT-PCR assay results indicated that GbHMGR belonged to a small gene family, and expressed in a tissue-specific manner with a low level expression being only found in root. The potential significance of GbHMGR gene was also discussed.
Shen
Guoan
G
Plant Biotechnology Research Center, Shanghai Key Laboratory of Biotechnology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Institute of Systems Biology, Shanghai Jiao Tong University, Shanghai 200030, China.
Pang
Yongzhen
Y
Wu
Weisheng
W
Liao
Zhihua
Z
Zhao
Lingxia
L
Sun
Xiaofen
X
Tang
Kexuan
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D020441:Ginkgo biloba / Q000201:enzymology* / Q000235:genetics; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D018517:Plant Roots / Q000201:enzymology* / Q000235:genetics; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent