Sleeping Beauty transposon system--future trend in T-cell-based gene therapies?
The Sleeping Beauty (SB) transposon system can mediate stable gene transfer and expression in primary human T cells. Optimal in vitro conditions for maximum gene transfer efficiencies have been developed with regard to further application of the SB transposon system in T cell based gene therapies. This raises the question of whether or not the SB transposon system is a convincing alternative for virus-mediated gene transfer based on the currently available data. Here, we will discuss controversial safety and efficacy issues in transposon and viral gene transfer technology.
Frommolt
Ruth
R
Department of Hematology & Oncology, Johannes Gutenberg University of Mainz, Langenbeckstr. 1, 55101 Mainz, Germany. frommolt@uni-mainz.de
Rohrbach
Florian
F
Theobald
Matthias
M
D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D013601:T-Lymphocytes / Q000378:metabolism*; D014162:Transfection; D019076:Transgenes / Q000502:physiology; D019895:Transposases / Q000235:genetics* / Q000378:metabolism
D019895:Transposases; C494471:sleeping beauty transposase, human