title: Characteristics of Ca2+-exocytosis coupling in isolated mouse pancreatic beta cells.
abstract: OBJECTIVE
  To characterize Ca2+-stimulated exocytosis in isolated mouse pancreatic beta cells.
  
  
  METHODS
  An improved method was described for isolation of mouse pancreatic beta cells by collagenase P. The Ca2+ channel current and the membrane capacitance were examined by using the whole-cell patch clamp recording technique.
  
  
  RESULTS
  Using depolarization and flash photolysis of caged Ca2+ to induce Ca2+-dependent exocytosis in beta cell from KM mouse, we have explored the characteristics of the Ca2+ channel current and the relationship between Ca2+ signals and exocytosis. The averaged peak Ca2+ current measured at +20 mV was -60+/-6 pA (n=13).
  
  
  CONCLUSIONS
  We characterized three kinetically different pools of vesicles in mouse pancreatic beta cells, namely an immediately releasable pool, a readily releasable pool, and a reserve pool.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ge
authors.forename: Yong-ming
authors.initials: YM
authors.lastname: Dong
authors.forename: Zhi-tao
authors.initials: ZT
authors.lastname: Hu
authors.forename: Zheng-xing
authors.initials: ZX
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D020013:Calcium Signaling; D002478:Cells, Cultured; D004705:Endocytosis / Q000502:physiology; D005089:Exocytosis / Q000502:physiology*; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice
chemical_list: D020746:Calcium Channels, L-Type; D002118:Calcium