title: Assembly and role of pili in group B streptococci.
abstract: Streptococcus agalactiae[group B streptococcus (GBS)] is the leading cause of neonatal pneumonia, sepsis and meningitis. An in silico genome analysis indicated that GBS strain NEM316 encodes five putative sortases, including the major class A sortase enzyme and four class C sortases. The genes encoding the class C sortases are tandemly arranged in two different loci, srtC1-C2 and srtC3-C4, with a similar genetic organization and are thought to be involved in pilus biosynthesis. Each pair of sortase genes is flanked by LPXTG protein encoding genes, two upstream and one downstream, and a divergently transcribed regulatory gene located upstream from this locus. We demonstrated that strain NEM316 expresses only the srtC3-C4 locus, which encodes three surface proteins (Gbs1474, Gbs1477 and Gbs1478) that polymerize to form appendages resembling pili. Structural and functional analysis of this locus revealed that: (i) the transcriptional activator RogB is required for expression of the srtC3-C4 operon; (ii) Gbs1477, and either SrtC3 or SrtC4 are absolutely required for pilus biogenesis; and (iii) GBS NEM316 pili are composed of three surface proteins, Gbs1477, the bona fide pilin which is the major component, Gbs1474, a minor associated component, and Gbs1478, a pilus-associated adhesin. Surprisingly, pilus-like structures can be formed in the absence of the two minor components, i.e. the putative anchor Gbs1474 or the adhesin Gbs1478. Adherence assays showed that Gbs1478 confers adhesive capacity to the pilus. This study provides the first evidence that adhesive pili are also present in Gram-positive pathogens.
authors.affiliation: Unité de Biologie des Bactéries Pathogènes à Gram-Positif Institut Pasteur, URA CNRS 2172, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France. sdramsi@pasteur.fr
authors.forename: Shaynoor
authors.initials: S
authors.lastname: Dramsi
authors.forename: Elise
authors.initials: E
authors.lastname: Caliot
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bonne
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Guadagnini
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Prévost
authors.forename: Mila
authors.initials: M
authors.lastname: Kojadinovic
authors.forename: Lila
authors.initials: L
authors.lastname: Lalioui
authors.forename: Claire
authors.initials: C
authors.lastname: Poyart
authors.forename: Patrick
authors.initials: P
authors.lastname: Trieu-Cuot
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics* / Q000378:metabolism*; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism*; D004847:Epithelial Cells / Q000382:microbiology; D036781:Fimbriae Proteins / Q000032:analysis / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D013292:Streptococcus agalactiae / Q000378:metabolism / Q000472:pathogenicity* / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; C509822:sortase C; D036781:Fimbriae Proteins; D019881:Aminoacyltransferases; D003546:Cysteine Endopeptidases