Identification of Taylorella equigenitalis responsible for contagious equine metritis in equine genital swabs by direct polymerase chain reaction.
A direct-PCR assay was developed for the rapid detection of Taylorella equigenitalis, a Gram-negative bacterium responsible for contagious equine metritis (CEM) in Equidae. The bacteria may be detected in equine genital swabs without need for a preliminary step of DNA extraction or bacterial isolation. Specificity was determined with 125 isolates of T. equigenitalis, 24 isolates of Taylorella asinigenitalis, five commensal bacteria of the genital tract and a facultative intracellular pathogen of foals found in large concentration in soil. Our PCR is specific and amplified a 413-bp 16S ribosomal DNA product only in all T. equigenitalis.
Duquesne
F
F
AFSSA, Laboratoire d'Etudes et de Recherches en Pathologie Equine, IPC, Goustranville, 14430 Dozulé, France.
Pronost
S
S
Laugier
C
C
Petry
S
S
D000818:Animals; D004716:Endometritis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D005836:Genitalia, Female / Q000382:microbiology*; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology*; D006736:Horses; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D020625:Taylorella equigenitalis / Q000235:genetics* / Q000302:isolation & purification*