Chemiluminescence flow-injection analysis of beta-lactam antibiotics using the luminol-permanganate reaction.
A new flow injection chemiluminescence (CL) method has been developed for the determination of six beta-lactam antibiotics, including amoxicillin, cefadroxil, cefoperazone sodium, cefazolin sodium, cefradine and ceftriaxone sodium. When the antibiotic was injected into a stream of KMnO4 with alkaline luminol, a strong CL signal was produced. The method allows the measurements of 0.1-50.0 mg/L amoxicillin, 0.1-80.0 mg/L cefadroxil, 1.0-30.0 mg/L cefoperazone sodium, 1.0-30.0 mg/L cefazolin sodium, 3.0-50.0 mg/L cefradine and 3.0-50.0 mg/L ceftriaxone sodium. The detection limits are 0.05 mg/L for amoxycillin, 0.05 mg/L for cefadroxil, 0.4 mg/L for cefoperazonum sodium, 0.4 mg/L for cefazolin sodium, 0.8 mg/L for cefradine and 0.8 mg/L for ceftriaxone sodium. The relative standard deviations in 11 repeated measurements are 0.6%, 0.8%, 1.5%, 1.2%, 0.4% and 0.3% for 3.0 mg/L amoxicillin, 1.0 mg/L cefadroxil, 10.0 mg/L cefoperazone sodium, 10.0 mg/L cefazolin sodium, 10.0 mg/L cefradine and 10.0 mg/L ceftriaxone sodium, respectively. The method was successfully applied to the determination of amoxicillin in pharmaceutical preparations. A possible CL reaction mechanism is also discussed.
Li
Yinhuan
Y
School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
Lu
Jiuru
J
D000658:Amoxicillin / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002434:Cefadroxil / Q000032:analysis; D002437:Cefazolin / Q000032:analysis; D002438:Cefoperazone / Q000032:analysis; D002443:Ceftriaxone / Q000032:analysis; D002515:Cephradine / Q000032:analysis; D017022:Flow Injection Analysis / Q000379:methods; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D011196:Potassium Permanganate / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D047090:beta-Lactams / Q000032:analysis*
D000900:Anti-Bacterial Agents; D012996:Solutions; D047090:beta-Lactams; D011196:Potassium Permanganate; D002434:Cefadroxil; D008165:Luminol; D002443:Ceftriaxone; D002438:Cefoperazone; D000658:Amoxicillin; D002515:Cephradine; D002437:Cefazolin