Effects of syringe material, sample storage time, and temperature on blood gases and oxygen saturation in arterialized human blood samples.
BACKGROUND
The practice of on-ice storage of arterial-blood samples in plastic syringes for delayed analysis continues, and the effects of storage time and temperature on the measurement of blood-oxygen-saturation values (S(aO2)) have not been adequately described.


OBJECTIVE
To determine the effects of syringe material, storage time, and storage temperature on normal arterialized blood gas and S(aO2) values.


METHODS
We used a temperature-controlled extracorporeal circuit to "arterialize" 500 mL of fresh, whole human blood at 37 degrees C, and we used certified calibration gases of 12% O2 and 5% CO2 to produce normal blood-gas values. From that arterialized blood we took 90 samples and randomly assigned them to 6 groups, until there were 15 samples in each group. The groups were (1) plastic syringe, analyzed immediately, (2) plastic syringe, stored 30 min at 0-4 degrees C, (3) plastic syringe, stored 30 min at 22 degrees C, (4) glass syringe, analyzed immediately, (5) glass syringe, stored 30 min at 0-4 degrees C, and (6) glass syringe, stored 30 min at 22 degrees C.


RESULTS
Compared to the samples that were analyzed immediately, the P(O2) of the samples stored in plastic syringes for 30 min at 22 degrees C and at 0-4 degrees C was significantly higher, with a clinically important magnitude of 11.9-13.7 mm Hg. The P(CO2) of blood stored in glass for 30 min at 0-4 degrees C was significantly lower, although the magnitude of the difference (1.5 mm Hg) was not clinically important. There were no statistically significant differences in pH or oxygen saturation among the 6 groups.


CONCLUSIONS
For accurate arterial-blood-gas results, samples drawn in plastic syringes should be analyzed immediately. If the analysis is going to be delayed, the samples should be drawn and stored in glass.
Knowles
Thomas P
TP
Cardiothoracic Anesthesia Department, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA. knowlet@ccf.org
Mullin
Rory A
RA
Hunter
Jefferson A
JA
Douce
F Herbert
FH
D000704:Analysis of Variance; D001784:Blood Gas Analysis / Q000379:methods*; D001793:Blood Preservation / Q000295:instrumentation*; D002245:Carbon Dioxide / Q000097:blood; D005112:Extracorporeal Circulation; D005898:Glass; D006801:Humans; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D010969:Plastics; D013594:Syringes; D013696:Temperature; D013997:Time Factors
D010969:Plastics; D002245:Carbon Dioxide; D010100:Oxygen