title: Intelligent design: the de novo engineering of proteins with specified functions.
abstract: One of the principal successes of de novo protein design has been the creation of small, robust protein-cofactor complexes which can serve as simplified models, or maquettes, of more complicated multicofactor protein complexes commonly found in nature. Different maquettes, generated by us and others, recreate a variety of aspects, or functional elements, recognized as parts of natural enzyme function. The current challenge is to both expand the palette of functional elements and combine and/or integrate them in recreating familiar enzyme activities or generating novel catalysis in the simplest protein scaffolds.
authors.affiliation: Johnson Research Foundation and Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Koder
authors.forename: P Leslie
authors.initials: PL
authors.lastname: Dutton
mesh_terms: D002384:Catalysis; D018360:Crystallography, X-Ray; D004798:Enzymes / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D008954:Models, Biological; D008958:Models, Molecular; D010100:Oxygen / Q000737:chemistry; D010455:Peptides; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry
chemical_list: D004798:Enzymes; D010455:Peptides; D011506:Proteins; D010100:Oxygen