Effects of recombinant human growth hormone on growth of human gastric carcinoma xenograft model in nude mice.
OBJECTIVE
To study effects of recombinant human growth hormone (rhGH) on growth of a human gastric carcinoma cell in vivo.


METHODS
Experimental mice were divided into control group, rhGH group, oxaliplatin (L-OHP) group and rhGH+L-OHP group. Cultured human gastric carcinoma cells BGC823 were inoculated into right axilla of nude mice and carcinoma xenograft model was established successfully. Inhibitory rate of xenograft tumor growth was estimated by measuring tumor volume; expression of proliferating cell nuclear antigen (PCNA), Bax and Bcl-2 proteins of xenograft tumor was detected using immunohistochemical S-P method.


RESULTS
Tumor growth inhibitory rate, the positive expression rate of PCNA, Bax and Bcl-2 were 49.3%, 58.2%, 65.2% and 59.2% in rhGH+L-OHP group respectively; 46.6%, 62.5%, 59.7% and 64.7% in L-OHP group; 5.0%, 82.7%, 23.2% and 82.2% in rhGH group and 0, 77.8%, 23.5% and 80.3% in control group. There was significant difference between rhGH+L-OHP group (or L-OHP group ) and control group or rhGH group (P < 0.05), whereas there were no significant differences (P > 0.05) between L-OHP group and rhGH+L-OHP group and between rhGH group and control group.


CONCLUSIONS
rhGH does not accelerate the proli-feration of human gastric cancer cell in vivo.
Liang
Dao-Ming
DM
Department of Emergency Surgery of Second Affiliated Hospital, Kunming Medical College, Yunnan Province, China.
Chen
Jia-Yong
JY
Zhang
Yi
Y
Gan
Ping
P
Lin
Jie
J
Chen
An-Bao
AB
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019382:Human Growth Hormone / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D013274:Stomach Neoplasms / Q000737:chemistry / Q000188:drug therapy / Q000473:pathology*; D023041:Xenograft Model Antitumor Assays / Q000379:methods; D051028:bcl-2-Associated X Protein / Q000032:analysis
D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D019382:Human Growth Hormone