Nonhuman sialic acid Neu5Gc is very low in human embryonic stem cell-derived neural precursors differentiated with B27/N2 and noggin: implications for transplantation.
A concern recently has been raised that human embryonic stem cell (HESC) lines cultured with currently available methods may have limited clinical usefulness due to the immunogenicity of the nonhuman sialic acid Neu5Gc incorporated into their membranes during culturing. We find this concern has little relevance to neural differentiation protocols with B27/N2/noggin because of the gradual decline of Neu5Gc to less than 1% in differentiating cells upon switching to B27/N2 medium.
Nasonkin
Igor O
IO
Department of Pathology, Division of Neuropathology, Baltimore, MD 21205, USA. igor.nasonkin@jhmi.edu
Koliatsos
Vassilis E
VE
D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007621:Karyotyping; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012794:Sialic Acids / Q000378:metabolism*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D000099176:Noggin Protein
D019208:Brain-Derived Neurotrophic Factor; D002352:Carrier Proteins; D016895:Culture Media, Serum-Free; D016222:Fibroblast Growth Factor 2; D012794:Sialic Acids; D000099176:Noggin Protein