Two-dimensional LNA/DNA arrays: estimating the helicity of LNA/DNA hybrid duplex.
We measured the helical repeats of a non-natural nucleic acid, locked nucleic acid (LNA), by incorporating LNA strands into the outer arms of a DNA double crossover (DX) molecule; atomic force microscopy (AFM) imaging of the two-dimensional (2D) arrays self-assembled from these DX molecules allows us to derive the helical repeat of the LNA/DNA hetero-duplex to be 13.2 +/- 0.9 base pairs per turn.
Rinker
Sherri
S
Department of Chemistry & Biochemistry and Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA. hao.yan@asu.edu.
Liu
Yan
Y
Yan
Hao
H
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000648:ultrastructure*; D013696:Temperature
D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; C477371:locked nucleic acid; D004247:DNA