title: Complement activation by sulfonated poly(ethylene glycol)-acrylate copolymers through alternative pathway.
abstract: Previously, novel poly(ethylene glycol) (PEG) and sulfonated PEG acrylate (PEG-SO(3)A/OA) copolymers were prepared as coating and/or blending materials for biomedical applications. Surfaces modified with copolymers exhibited increased anti-coagulation properties and decreased plasma adsorption level due to increased hydrophilic properties and reorientation characteristics of PEG/PEG-SO(3)A chains in water phase. As continuation study, anti-complement effects of PEG-SO(3)/OA copolymers were investigated in vitro, and compared with those of low-density polyethylene (LDPE) and PEG/OA. C3 activation by PEG-SO(3)/OA samples was lower than that by PEG/OA samples, which was attributed to decreased surface nucleophile level of samples. PEG-SO(3)/OA samples increased inhibition of Bb production, resulting in decreased C5 activation. Owing to reduced activations of C3 and C5, PEG-SO(3)/OA samples markedly decreased SC5b-9 levels in plasma.
authors.affiliation: Department of Therapeutic Radiology, Uijeongbu St. Mary's Hospital, College of Medicine, Catholic University, Uijeongbu 480-130, Korea.
authors.forename: Hong Seok
authors.initials: HS
authors.lastname: Jang
authors.forename: Kyu Eun
authors.initials: KE
authors.lastname: Ryu
authors.forename: Woong Shick
authors.initials: WS
authors.lastname: Ahn
authors.forename: Heung Jae
authors.initials: HJ
authors.lastname: Chun
authors.forename: Hyung
authors.initials: H
authors.lastname: Dal Park
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Park
authors.forename: Young Ha
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000179:Acrylates / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible; D015936:Complement C5a / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D003170:Complement Pathway, Alternative / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000179:Acrylates; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D015938:Complement Membrane Attack Complex; D011092:Polyethylene Glycols; D015936:Complement C5a