[The analysis of cellular elements in coelomic fluid during early regeneration of of the starfish Asterias rubens L].
Three main cell types were found in the coelomic fluid (CF) of intact starfishes: agranulocytes (55-80%) varying in size and form (spherical and ovoid) and with occasional pseudopodia, granulocytes (15-45%), and small cells (up to 2 %) with a high nuclear-cytoplasmic ratio. The starfish response to injury depends on the degree of coelomic fluid loss. After a slight wounding, when only insignificant portion of CF is lost, the cellular composition of circulating fluid changed only slightly. Unlike, a significant injury resulted in rising the share of small cells, regarded presumably as young cells. Besides, after injury the functional characteristics of SF also changed: the proportion of cells with decondensed chromatin and stained nucleoli increased, and coelomocytes acquired ability to form nets at adhesion. Moreover, some new cell types can be found (fusiform cells), with granulocyte proportion in nets increasing. We suppose that after slight wounding circulating coelomocytes may restore from the existing store of differentiated cells beyond the circulation, whereas after significant injury young undifferentiated coelomocytes are involved in the process of restoration.
Kozlova
A B
AB
Petukhova
O A
OA
Pinaev
G P
GP
D000818:Animals; D047271:Asterias / Q000166:cytology* / Q000502:physiology; D002448:Cell Adhesion; D002452:Cell Count; D006098:Granulocytes / Q000166:cytology; D012038:Regeneration; D013997:Time Factors