title: [Construction of three-dimensional models of Arabidopsis thaliana FtsZ-proteins on basis of crystal structure of archaebacterial FtsZ-GDP complex].
abstract: Three-dimensional models of FtsZ-protein complexes with GDP from Arabidopsis thaliana L. localized in cytosol (Entrez database code NP190843) and in chloroplasts (Entrez database code AAA82068) were developed. Crystal structure of the FtsZ-GDP complex from archaea Methanococcus jannaschii (PDB-code 1FSZ) was used as a matrix. Secondary structures of computed models contain ten beta-strands. A chloroplast form of FtsZ-protein has ten alpha-helices and four 3(10)-helices, whereas cytosolic form of protein has nine and three structures correspondently and neither a0-helix before nucleotide-binding domain nor C-terminal 3(10)-helix in secondary domain. The T2-loop of nucleotide-binding pocket of chloroplast form of FtsZ-ptotein in position 111 contains non-charged alanin residue instead of the charged one which is typical for cytosolic and bacterial forms of proteins. At low sequence homology of FtsZ-proteins (approximately 47%) the developed models demonstrate high coincidence with matrix both in the structures of nucleotide-binding pocket and in the whole molecule. The models are completely suitable for further studies of possible sites of binding with dinitroaniline herbicides.
authors.forename: O N
authors.initials: ON
authors.lastname: Demchuk
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Nyporko
authors.forename: Ia B
authors.initials: IaB
authors.lastname: Blium
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry* / Q000378:metabolism; D029681:Arabidopsis Proteins; D019843:Archaeal Proteins / Q000737:chemistry*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry*; D002736:Chloroplasts / Q000378:metabolism; D018360:Crystallography, X-Ray; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000737:chemistry*; D003600:Cytosol / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000096:biosynthesis / Q000737:chemistry*; D017017:Methanococcus / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D029681:Arabidopsis Proteins; D019843:Archaeal Proteins; D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C095113:FTSZ protein, Arabidopsis; C071525:FtsZ protein, Bacteria; D010940:Plant Proteins; D020558:GTP Phosphohydrolases