title: [Preparation and characterization of monoclonal antibody against DR5].
abstract: OBJECTIVE
  To prepare monoclonal antibodies(mAb) against DR5 and characterize their properties.
  
  
  METHODS
  BALB/c mice were immunized with DR5, and then mAb was prepared by hybridoma technique. Ig subclass and specificity of mAbs was analyzed by ELISA and flow cytometry, respectively. The titres of mAbs in ascitic fluid, relative affinity and epitopes recognized by mAbs were determined by indirect ELISA.
  
  
  RESULTS
  Four hybridoma cell lines secreting anti DR5 mAbs were obtained. Their Ig subclass belonged to IgG1. The titers of 4 mAbs in ascitic fluid were 1x10(-4) - 5x10(-6). Affinity constant of mAbs were 1x10(9). They recognized 2 different epitopes on DR5 molecule.
  
  
  CONCLUSIONS
  Four mAbs against DR5 are prepared successfully, which provides useful reagent for clinical diagnosis and further research.
authors.affiliation: Research Institute of Immunology, Henan University, Kaifeng 475001, China. kfbaihling369@tom.com
authors.forename: Hui-ling
authors.initials: HL
authors.lastname: Bai
authors.forename: Yue-ping
authors.initials: YP
authors.lastname: Zhao
authors.forename: Guang-chao
authors.initials: GC
authors.lastname: Liu
authors.forename: Yuan-fang
authors.initials: YF
authors.lastname: Ma
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D019169:Jurkat Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000276:immunology*; D012995:Solubility
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand