Heteroduplex mapping of heat-resistant deletion mutants of bacteriophage t5.
The bacteriophage T5 is known to spontaneously generate deletion mutants (st mutants) exhibiting enhanced resistance to heat inactivation in citrate buffer. A series of such mutants has been isolated and the deletions visualized by electron microscopy of heteroduplex molecules. The deletions are found to cluster in one region of the chromosome.
Scheible
P P
PP
Department of Biology, University of Virginia, Charlottesville, Virginia 22901.
Rhoades
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