[Detection of the labile iron pool in leukemia cells and its significance].
To explore a rapid and easy method to detect labile iron of pool (LIP) in cells, HL-60 and K562 cells were cultured at a concentration 1 x 10(6)/ml in RPMI 1640 containing 10% heat-inactivated fetal bovine serum. The iron deprivation was induced by adding desferrioxamine (DFO) 10 - 100 micromol/L for 0 - 48 hours. The intracellular LIP was measured by probe calcein-AM. Calcein fluorescence was monitored in 1420 multilabel counter. The results indicated that when HL-60 and K562 cells were incubated with different concentrations of DFO, the calcein fluorescence intensity was higher than that of control group at 12, 24 and 48 hours (P < 0.05). Fluorescence value of representing LIP in DFO groups was lower than that in the control group. In conclusion, DFO can decrease LIP in leukemia cells. The approach used in this study may provide a simple and reliable method for detection of intracellular iron homeostasis.
Jia
Guo-Cun
GC
Department of Pediatric Hematology, Zhengzhou Children Hospital, Zhengzhou 450053, China.
Gao
Ju
J
Liao
Qing-Kui
QK
Li
Feng-Yi
FY
Yuan
Li-Xing
LX
He
Bin
B
D027682:Cation Transport Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D003676:Deferoxamine / Q000494:pharmacology; D005452:Fluoresceins; D005456:Fluorescent Dyes; D018922:HL-60 Cells; D006801:Humans; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000032:analysis* / Q000378:metabolism; D035925:Iron-Regulatory Proteins / Q000378:metabolism*; D020014:K562 Cells
D027682:Cation Transport Proteins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007502:Iron Chelating Agents; D035925:Iron-Regulatory Proteins; C085925:calcein AM; D007501:Iron; D003676:Deferoxamine