title: Functional expression of the astaxanthin biosynthesis genes from a marine bacterium, Paracoccus haeundaensis.
abstract: The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product has been purified to homogeneity. Their molecular characteristics, including enzymatic activities, are reported here.
authors.affiliation: Department of Microbiology, Pukyong National University, Busan, 608-737, South Korea.
authors.forename: Jae Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Kim
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors / Q000096:biosynthesis; D008967:Molecular Biology / Q000379:methods; D010230:Paracoccus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D024341:Xanthophylls / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D024341:Xanthophylls; D002338:Carotenoids; C005948:astaxanthine