An eco-friendly and sustainable process for enzymatic hydrolysis of penicillin G in cloud point system.
Enzymatic hydrolysis of penicillin G by immobilized penicillin acylase in a nonionic surfactant mediated cloud point system was presented. The effect of the operation parameters on equilibrium pH of this enzymatic hydrolysis process without pH control was examined. A relatively high equilibrium pH in cloud point system without pH control can be obtained. The feasibility of recycling utilization of the nonionic surfactant, a novel green solvent, was also investigated experimentally. Enzymatic hydrolysis of penicillin G in a discrete semi-batch mode, which simulates a semi-continuous process, envisages a completely eco-friendly, sustainable and efficient process for production of 6-aminopenicillanic acid.
Wang
Li
L
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Wang
Zhilong
Z
Xu
Jian-He
JH
Bao
Da
D
Qi
Hanshi
H
D003247:Conservation of Natural Resources; D004787:Environmental Pollution / Q000517:prevention & control*; D004800:Enzymes, Immobilized / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010399:Penicillin Amidase / Q000737:chemistry*; D010400:Penicillin G / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
D004800:Enzymes, Immobilized; D013501:Surface-Active Agents; D010399:Penicillin Amidase; D010400:Penicillin G