A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development.
The potential role of endogenous triglyceride in bovine oocyte maturation and preimplantation development has been investigated. Bovine immature oocytes were recovered from abattoir-derived ovaries, matured and fertilised in vitro and the zygotes grown to the blastocyst stage in SOFaaBSA. Methyl palmoxirate (MP) blocks the oxidation of fatty acids by inhibiting mitochondrial carnitine palmitoyltransferase A. The development of zygotes exposed to MP during oocyte maturation, and of zygotes exposed to MP during embryo culture has been assessed in terms of oxygen consumption by oocytes and embryos during a 4-6 hr incubation period in the presence of MP and as blastocyst formation and cell number. Immature oocytes exposed to MP during maturation had reduced capacity to form blastocysts after fertilisation; the same effect was apparent, but to a lesser extent, in zygotes exposed to MP during embryo development. Oxygen consumption values of oocytes and blastocysts in the absence of exogenous substrates were similar to those in control medium containing nutrients. MP-inhibited oxygen consumption of immature oocytes, mature oocytes, cleavage stages embryos and blastocysts by 64, 45, 12 and 13%, respectively. The data are consistent with a role for triglyceride as a key energy source during bovine oocyte maturation and potentially, during preimplantation embryo development.
Ferguson
Elizabeth M
EM
Department of Biology, University of York, York, United Kingdom.
Leese
Henry J
HJ
D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism*; D004852:Epoxy Compounds / Q000037:antagonists & inhibitors / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D009866:Oogenesis; D010100:Oxygen / Q000378:metabolism; D011422:Propionates / Q000037:antagonists & inhibitors / Q000494:pharmacology; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism* / Q000494:pharmacology; D015053:Zygote / Q000166:cytology / Q000187:drug effects
D004852:Epoxy Compounds; D011422:Propionates; D014280:Triglycerides; C017522:methyl 2-tetradecylglycidate; D010100:Oxygen