The effect of conventional and sustained delivery of thymoquinone and levodopa on SH-SY5Y human neuroblastoma cells.
The objective of this study was to determine the effects of thymoquinone on the viability and metabolic activity of SH-SY5Y human neuroblastoma cells alone or challenged with levodopa (L-dopa) using conventional and sustained drug delivery routes. The findings suggest that thymoquinone may protect SH-SY5Y cells against L-dopa toxicity to some degree although the exact mechanism for this is unknown. Thymoquinone may be a viable option to prevent quinone formation as a result of L-dopa auto-oxidation and could be investigated as a neuroprotective agent given the fact that inflammatory etiologies are strongly implemented in the pathogenesis of Parkinson's disease.
Martin
Tina Mitchell
TM
University of Mississippi Medical Center, Jackson, MS 39216-4505, USA.
Benghuzzi
Hamed
H
Tucci
Michelle
M
D000978:Antiparkinson Agents / Q000008:administration & dosage; D016227:Benzoquinones / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007980:Levodopa / Q000008:administration & dosage*; D009447:Neuroblastoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D018696:Neuroprotective Agents / Q000008:administration & dosage; D016896:Treatment Outcome
D000978:Antiparkinson Agents; D016227:Benzoquinones; D003692:Delayed-Action Preparations; D004338:Drug Combinations; D018696:Neuroprotective Agents; D007980:Levodopa; D006861:Hydrogen Peroxide; C003466:thymoquinone