Role of DNA replication and repair in thymineless death in Escherichia coli.
Inhibition of DNA replication with hydroxyurea during thymine starvation of Escherichia coli shows that active DNA synthesis is not required for thymineless death (TLD). Hydroxyurea experiments and thymine starvation of lexA3 and uvrA DNA repair mutants rule out unbalanced growth, the SOS response, and nucleotide excision repair as explanations for TLD.
Morganroth
Pamela A
PA
Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, USA.
Hanawalt
Philip C
PC
D004260:DNA Repair; D004261:DNA Replication; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D007700:Kinetics; D012329:RNA, Bacterial / Q000235:genetics; D013941:Thymine / Q000378:metabolism*; D014158:Transcription, Genetic
D012329:RNA, Bacterial; D013941:Thymine