[Evaluation of two methods for counting residual white blood cells in thrombocytaphoresis concentrates].
To evaluate flow-cytometry and Nageotte method for counting residual WBC in thrombocytaphoresis concentrates, their accuracies were determined by dilution studies separately; the repeatability was determined by measuring the interassay coefficient of variation for 14 replicates of a sample with known leukocyte concentration. 102 samples of leukocyte-depleted thrombocytaphoresis concentrates were detected by the above mentioned two methods, and the results were compared each other. The results showed that no difference was observed between two methods over a range of leukocyte concentrations from 0.8 to 10 WBC/microl (P > 0.05). In conclusion, flow-cytometry and Nageotte methods can be used for quality control of WBC-reduced blood components.
Wang
Yong-Jun
YJ
Blood Center of Zhejiang Province, Hangzhou 310006, China. wyjygm@yahoo.com.cn
Sheng
Zhuo-Lan
ZL
Xu
Jian
J
Huang
Bo-Li
BL
Hu
Qing-Feng
QF
Feng
Wu-Wei
WW
D016913:Blood Component Transfusion; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*; D047589:Leukocyte Reduction Procedures / Q000379:methods; D010983:Plateletpheresis