title: A simple, rapid and effective method for total RNA extraction from Lentinula edodes.
abstract: A rapid, inexpensive and reliable method for total RNA extraction from fruiting bodies of Lentinula edodes containing large quantities of polysaccharides and secondary metabolites is described. An initial extraction step using saturated NaCl solution facilitates the separation of nucleic acids from contaminants and, after further extraction with organic solvents and precipitation with 2-propanol, total RNA of high purity and suitable for applications such as cDNA synthesis, RT-PCR and Northern blot hybridization was obtained. The procedure may also have wider applicability for total RNA extraction from the tissues of other mushrooms.
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Li
authors.forename: Chuan-Hong
authors.initials: CH
authors.lastname: Tang
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Song
authors.forename: Ming-Jie
authors.initials: MJ
authors.lastname: Chen
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Feng
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Pan
mesh_terms: D015152:Blotting, Northern / Q000379:methods; D048690:Fruiting Bodies, Fungal / Q000737:chemistry* / Q000235:genetics; D012313:RNA / Q000302:isolation & purification*; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D020942:Shiitake Mushrooms / Q000737:chemistry* / Q000235:genetics
chemical_list: D012462:Saline Solution, Hypertonic; D012313:RNA