Exogenous pleiotrophin applied to lesioned nerve impairs muscle reinnervation.
Pleiotrophin (PTN) is a heparin-binding growth factor involved in nerve regeneration after peripheral nerve injury. After crush injury, PTN is found in distal nerve segments in several non-neural cell types, including Schwann cells, macrophages, and endothelial cells, but not in axons. To further clarify the role for PTN in nerve regeneration, we investigated the effects of PTN applied to lesioned peripheral nerve in vivo. PTN in a dose of 1 mg/kg impaired muscle reinnervation. Thus, gastrocnemius muscle failed to recover its contractile properties as assessed by in situ maximal isometric tetanic force. PTN also decreased non-neural cell densities and delayed macrophage recruitment in the distal crushed nerve. These results are discussed in the light of recent evidence that PTN is a multifunctional polypeptide.
Blondet
Brigitte
B
Laboratoire de Recherche sur la Croissance Cellulaire, la Réparation et la Régénération Tissulaires, UMR CNRS, No 7149, Université Paris 12, Avenue du Général de Gaulle, 94010, Créteil, France. blondet@univ-paris12.fr
Carpentier
Gilles
G
Ferry
Arnaud
A
Courty
José
J
D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D016207:Cytokines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007150:Immunohistochemistry; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000294:innervation; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000187:drug effects* / Q000254:growth & development
D002352:Carrier Proteins; D016207:Cytokines; C066845:pleiotrophin