title: Protein contamination of the Laryngeal Mask Airway and its relationship to re-use.
abstract: The Laryngeal Mask Airway is a reusable device for maintaining the patency of a patient's airway during general anaesthesia. The device can be reused after it has been cleaned and sterilized. Protein contamination of medical instruments is a concern and has been found to occur despite standard sterilization techniques. The reason for the concern relates to the possibility of the transmission of prions and the risk of developing a neurodegenerative disorder such as Creutzveldt-Jacob disease. The purpose of this study was to quantify the amount of protein contamination that occurs, and to relate this to the number of times the Laryngeal Mask Airway has been used. Fifty previously used Classic Laryngeal Masks were collected after routine sterilization and packaging. The devices were immersed in protein detecting stain and then visual inspection performed to assess the degree and distribution of the staining. The researcher was blinded to the number of times the Laryngeal Mask Airway had been used. Linear regression analysis of the degrees of staining of the airway revealed that protein contamination occurs after the first use of the device and this increases with each subsequent use. This finding highlights the concern that the currently used cleaning and sterilization methods do not prevent the accumulation of proteinaceous material on Laryngeal Mask Airways. Consideration should be given to the search for more efficient cleaning and sterilization techniques or the use of disposable devices.
authors.affiliation: Department of Anaesthetics, Southern Medical School University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Greenwood
authors.forename: N
authors.initials: N
authors.lastname: Green
authors.forename: G
authors.initials: G
authors.lastname: Power
mesh_terms: D004866:Equipment Contamination; D017743:Equipment Reuse; D004923:Erythrosine; D005456:Fluorescent Dyes; D006801:Humans; D017214:Laryngeal Masks; D017096:Prion Diseases / Q000635:transmission; D011506:Proteins / Q000032:analysis*; D013242:Sterilization
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D004923:Erythrosine