DNA polymerases for translesion DNA synthesis: enzyme purification and mouse models for studying their function.
This chapter discusses experimental methods and protocols for the purification and preliminary characterization of DNA polymerases that are specialized for the replicative bypass (translesion DNA synthesis) of base or other types of DNA damage that typically arrest high-fidelity DNA synthesis, with particular emphasis on DNA polymerase kappa (Polkappa from mouse cells). It also describes some of the methods employed in the evaluation of mouse strains defective in genes that encode these enzymes.
Fischhaber
Paula L
PL
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, USA.
McDaniel
Lisa D
LD
Friedberg
Errol C
EC
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D023421:Models, Animal; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014466:Ultraviolet Rays
D011994:Recombinant Proteins; D004247:DNA; D004259:DNA-Directed DNA Polymerase