Analysis of non-B DNA structure at chromosomal sites in the mammalian genome.
Changes at sites of genetic instability ultimately involve DNA repair pathways. Some sites of genetic instability in the mammalian genome appear to be unstable because they adopt a non-B DNA conformation. We describe two structural approaches for determination of whether a genomic region is configured in a non-B DNA conformation. Our studies indicate that at least some chromosomal fragile sites can be explained by such altered DNA conformations. One of the methods that we describe is called the bisulfite modification assay. This is a powerful assay because it provides information on individual DNA molecules. The second approach uses preexisting DNA structural reagents, but describes our specific application of them to analysis of DNA in vivo.
Raghavan
Sathees C
SC
Department of Pathology, University of Southern California, Keck School of Medicine, Los Angeles, USA.
Tsai
Albert
A
Hsieh
Chih-Lin
CL
Lieber
Michael R
MR
D000818:Animals; D001483:Base Sequence; D043283:Chromosome Fragile Sites; D004247:DNA / Q000737:chemistry*; D016678:Genome; D007202:Indicators and Reagents / Q000737:chemistry; D008322:Mammals / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction
D007202:Indicators and Reagents; D004247:DNA