title: Novel design of multicapillary arrays for high-throughput DNA sequencing.
abstract: A novel approach to design and optimize linear multicapillary arrays (LMCAs) for high-throughput DNA sequencing is proposed. A significant increase in the number of capillary lanes is obtained due to the use of composite insertions alternately placed between working capillaries of the array and a specific combination of refractive indices of the DNA separation matrix, capillary glass, the insertions and a medium which surrounds the capillary array. Theoretical and experimental studies showed that in conjunction with a dual-side laser illumination scheme, the proposed LMCA design allows a simultaneous uniform irradiation of as many as 550 working capillaries.
authors.affiliation: Electrical and Computer Engineering Department, State University of New York at Stony Brook, Stony Brook, NY, USA. atsupryk@ic.sunysb.edu
authors.forename: Andriy
authors.initials: A
authors.lastname: Tsupryk
authors.forename: Michael
authors.initials: M
authors.lastname: Gorbovitski
authors.forename: Evgeni A
authors.initials: EA
authors.lastname: Kabotyanski
authors.forename: Vera
authors.initials: V
authors.lastname: Gorfinkel
mesh_terms: D003198:Computer Simulation; D019075:Electrophoresis, Capillary; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D017422:Sequence Analysis, DNA / Q000379:methods*