title: A new mucin antibody/enzyme-linked lectin-sandwich assay of serum MUC5AC mucin for the diagnosis of cholangiocarcinoma.
abstract: We have previously used agarose gel electrophoresis and immunoblotting to qualitatively measure serum MUC5AC mucin for diagnosing cholangiocarcinoma. In this study, we developed a quantitative determination of serum MUC5AC by sandwich ELISA using MUC5AC mucin monoclonal antibody and soybean agglutinin. A cut-off value of the absorbance 0.074 was obtained from a complete statistical Receiver Operating Characteristic curves with an area under the curve=0.8141. The assay could discriminate cholangiocarcinoma patients from the controls with 71% sensitivity and 90% specificity. The test is simple to perform, reproducible, and probably used for detecting cholangiocarcinoma in a high-risk group or suspected patients.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chiang Mai Universtiy, Chiang Mai 50002, Thailand.
authors.forename: Wichuda
authors.initials: W
authors.lastname: Bamrungphon
authors.forename: Nalinee
authors.initials: N
authors.lastname: Prempracha
authors.forename: Nophawan
authors.initials: N
authors.lastname: Bunchu
authors.forename: Samreung
authors.initials: S
authors.lastname: Rangdaeng
authors.forename: Trichak
authors.initials: T
authors.lastname: Sandhu
authors.forename: Songphol
authors.initials: S
authors.lastname: Srisukho
authors.forename: Chanchai
authors.initials: C
authors.lastname: Boonla
authors.forename: Sopit
authors.initials: S
authors.lastname: Wongkham
mesh_terms: D000368:Aged; D001650:Bile Duct Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000097:blood*; D018281:Cholangiocarcinoma / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D037102:Lectins; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC; D009077:Mucins / Q000097:blood*; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D037102:Lectins; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D009077:Mucins