title: Self transglutaminase-based rapid coeliac disease antibody detection by a lateral flow method.
abstract: BACKGROUND
  The conventional coeliac disease antibody tests require patient's sera, and are laborious and time-consuming.
  
  
  OBJECTIVE
  To evaluate a newly developed rapid whole blood test in coeliac disease antibody detection, and its suitability for office use.
  
  
  METHODS
  Endogenous tissue transglutaminase found in red blood cells in a whole blood fingertip or venous sample is liberated upon haemolysis and complexes with tissue transglutaminase antibodies, if present. The complexes, captured by a lateral flow system, are visualized within 5 min. Stored samples from 121 untreated, 106 treated coeliac disease patients and 107 controls were evaluated and compared with serum endomysium and tissue transglutaminase antibody tests and histology; 150 patients were prospectively tested on site in the doctor's office.
  
  
  RESULTS
  The rapid test showed sensitivity (96.7%) comparable with the serum endomysium and tissue transglutaminase antibody tests from stored samples; specificity was slightly lower (93.5%). When tested on site the results were concordant in 96.7% of cases compared with endomysium and tissue transglutaminase antibody results. The test recognized the disappearance of tissue transglutaminase antibodies on a gluten-free diet.
  
  
  CONCLUSIONS
  The self tissue transglutaminase-based rapid test can be easily carried out from a fingertip blood sample on site in the physician's office for both coeliac disease case finding and dietary monitoring purposes.
authors.affiliation: Paediatric Research Centre, University of Tampere, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Raivio
authors.forename: K
authors.initials: K
authors.lastname: Kaukinen
authors.forename: E
authors.initials: E
authors.lastname: Nemes
authors.forename: K
authors.initials: K
authors.lastname: Laurila
authors.forename: P
authors.initials: P
authors.lastname: Collin
authors.forename: J B
authors.initials: JB
authors.lastname: Kovács
authors.forename: M
authors.initials: M
authors.lastname: Mäki
authors.forename: I R
authors.initials: IR
authors.lastname: Korponay-Szabó
mesh_terms: D000293:Adolescent; D000906:Antibodies / Q000097:blood*; D002446:Celiac Disease / Q000175:diagnosis*; D002648:Child; D005260:Female; D006801:Humans; D007159:Immunologic Tests / Q000379:methods / Q000592:standards; D008297:Male; D019095:Point-of-Care Systems / Q000592:standards*; D012648:Self Care / Q000592:standards; D011503:Transglutaminases / Q000097:blood* / Q000276:immunology
chemical_list: D000906:Antibodies; D011503:Transglutaminases