title: [Protection of human renal tubular epithelial cells (HK-2 cells) from hypoxia-reoxygenation damage by recombinant adenovirus containing hCGPx gene].
abstract: OBJECTIVE
  To study the protective effect of human renal tubular epithelial cells (HK-2 cells) from hypoxia-reoxygenation damage by transfection of recombinant adenovirus-mediated human cytosolic glutathione peroxidase(hCGPx) gene.
  
  
  METHODS
  Recombinant pGEM-T vector containing hCGPx cDNA and pACCMV-pLpA adenovirus shuttle plasmid was constructed. Then the shuttle plasmid pACCMV-hCGPx and pJM17 were co-transfected into 293 cells and recombinant adenovirus AdCMV-hCGPx was obtained. Cultured HK-2 cells were transfected with AdCMV-hCGPx or vacant recombinant adenovirus (control). The expression ratio of transfected hCGPx gene were studied. Cell viability, the percentage of apoptosis and death were evaluated after hypoxia-reoxygenation damage.
  
  
  RESULTS
  The expression ratio of hCGPx gene was higher in the AdCMV-hCGPx transfected cells than that in the control group (P<0.01). After hypoxia-reoxygenation damage, the viability of hCGPx gene transfected cells was significantly higher than that of control and the percentage of apoptosis and death of hCGPx transfected cells was significantly lower than that of control.
  
  
  CONCLUSIONS
  The transfection of hCGPx mediated by recombinant adenovirus could protect renal tubular epithelial cells from hypoxia-reoxygenation damage in vitro.
authors.affiliation: Department of Kidney Transplantation, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, China. hlxiang@163.com
authors.forename: He-li
authors.initials: HL
authors.lastname: Xiang
authors.forename: Wu-jun
authors.initials: WJ
authors.lastname: Xue
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Pu-xun
authors.initials: PX
authors.lastname: Tian
authors.forename: Yan
authors.initials: Y
authors.lastname: Teng
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Pan
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Ding
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis; D003600:Cytosol / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004274:DNA, Recombinant / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D007684:Kidney Tubules / Q000473:pathology*; D010100:Oxygen / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D004274:DNA, Recombinant; D005979:Glutathione Peroxidase; D004262:DNA Restriction Enzymes; D010100:Oxygen