Improvement of expression level and bioactivity of soluble tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL) by a novel zinc ion feeding strategy.
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (Apo2L/TRAIL) is a new member of the TNF superfamily. In this work, the key role of Zn(2+) in high-level expression of soluble TRAIL was confirmed. The yield of soluble TRAIL reached 1.6 g l(-1) using a novel, two-stage Zn(2+) feeding strategy, and the accumulation of TRAIL inclusion bodies decreased. Furthermore, the purified TRAIL showed stronger cytotoxicity activity against human pancreatic 1990 tumor cells as the molar ratio of Zn(2+) to TRAIL monomer was 2 in purified TRAIL solution.
Sun
Ai-You
AY
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, PR China.
Shen
Ya-Ling
YL
Yin
Ji-Cheng
JC
Zhang
Hong
H
Tang
Ya-Nan
YN
Wei
Dong-Zhi
DZ
D017209:Apoptosis / Q000502:physiology; D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012995:Solubility; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism* / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology*
D003470:Culture Media; D011994:Recombinant Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; D015032:Zinc