Mobility of mass-reared diapaused and nondiapaused Cydia pomonella (Lepidoptera: Tortricidae): effect of different constant temperatures and lengths of cold storage.
Desirable behavioral attributes in mass-reared insects should include the ability to perform favorably under the various environmental conditions they encounter upon release in the field. Insect quality also may be influenced by storage conditions and storage duration before field release. We studied the effects of three different constant ambient temperatures (15, 20, and 25 degrees C) and different lengths of adult cold storage (0, 24, 48, and 72 h at 2 degrees C) on the locomotor activity of adult Cydia pomonella (L.) mass reared through diapause or standard production protocols. Mobility was assessed in actographs housed in a climate controlled chamber; tests lasted 24 h. We found that adult mobility was significantly higher for both males and females at 25 and 20 degrees C than at 15 degrees C. There were no significant differences in mobility in moths reared through diapause or nondiapaused production protocols. In addition, temporal analysis of the data revealed a significant shift in the diel patterns of activity for both genders when adults were tested at the three different temperatures. Moths exposed to the lower temperature shifted their activity pattern from evening to mid-afternoon, which may be an adaptive behavior to take advantage of the expected warmest period of the day. Diapaused adults were significantly less mobile when stored in the cold (24, 48, or 72 h of storage at 2 degrees C) than were diapaused adults that did not experience cold storage (0 h). However, length of time in cold storage did not significantly influence the mobility of adult codling moths reared through standard production protocols.
Bloem
Stephanie
S
Institute of Plant Sciences/Applied Entomology, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland.
Carpenter
James E
JE
Dorn
Silvia
S
D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D003080:Cold Temperature; D005260:Female; D008297:Male; D009036:Moths / Q000502:physiology*; D009043:Motor Activity / Q000502:physiology*; D010572:Pest Control, Biological / Q000379:methods; D017440:Photoperiod; D013696:Temperature