Pinoresinol-lariciresinol reductase gene expression and secoisolariciresinol diglucoside accumulation in developing flax (Linum usitatissimum) seeds.
The transcription activity of the pinoresinol-lariciresinol reductase (PLR) gene of Linum usitatissimum (so-called LuPLR), a key gene in lignan synthesis, was studied by RT-PCR and promoter-reporter transgenesis. The promoter was found to drive transcription of a GUSint reporter gene in the seed coats during the flax seed development. This fitted well with the tissue localization monitored by semi-quantitative RT-PCR of LuPLR expression. Accumulation of the main flax lignan secoisolariciresinol diglucoside was coherent with LuPLR expression during seed development. This three-way approach demonstrated that the LuPLR gene is expressed in the seed coat of flax seeds, and that the synthesis of PLR enzyme occurs where flax main lignan is found stored in mature seeds, confirming its involvement in SDG synthesis.
Hano
C
C
Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, Antenne Scientifique Universitaire de Chartres, 21 rue de Loigny la Bataille, 28000, Chartres, France.
Martin
I
I
Fliniaux
O
O
Legrand
B
B
Gutierrez
L
L
Arroo
R R J
RR
Mesnard
F
F
Lamblin
F
F
Lainé
E
E
D001483:Base Sequence; D002072:Butylene Glycols / Q000378:metabolism*; D003001:Cloning, Molecular; D018744:DNA, Plant; D019597:Flax / Q000196:embryology / Q000201:enzymology* / Q000378:metabolism; D017705:Lignans / Q000378:metabolism*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000201:enzymology*
D002072:Butylene Glycols; D018744:DNA, Plant; D017705:Lignans; D010088:Oxidoreductases; C084968:lariciresinol reductase; C084969:pinoresinol reductase; C060283:secoisolariciresinol