High-throughput biomonitoring of dioxins and polychlorinated biphenyls at the sub-picogram level in human serum.
We report on the use of a state-of-the-art method for the measurement of selected polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and polychlorinated biphenyls in human serum specimens. The sample preparation procedure is based on manual small size solid-phase extraction (SPE) followed by automated clean-up and fractionation using multi-sorbent liquid chromatography columns. SPE cartridges and all clean-up columns are disposable. Samples are processed in batches of 20 units, including one blank control (BC) sample and one quality control (QC) sample. The analytical measurement is performed using gas chromatography coupled to isotope dilution high-resolution mass spectrometry. The sample throughput corresponds to one series of 20 samples per day, from sample reception to data quality cross-check and reporting, once the procedure has been started and series of samples keep being produced. Four analysts are required to ensure proper performances of the procedure. The entire procedure has been validated under International Organization for Standardization (ISO) 17025 criteria and further tested over more than 1500 unknown samples during various epidemiological studies. The method is further discussed in terms of reproducibility, efficiency and long-term stability regarding the 35 target analytes. Data related to quality control and limit of quantification (LOQ) calculations are also presented and discussed.
Focant
Jean-François
JF
CART, Mass Spectrometry Laboratory, University of Liège, Allée de la Chimie 3, B-6c Sart-Tilman, B-4000 Liège, Belgium. JF.Focant@ulg.ac.be
Eppe
Gauthier
G
Massart
Anne-Cécile
AC
Scholl
Georges
G
Pirard
Catherine
C
De Pauw
Edwin
E
D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods*; D004147:Dioxins / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D011078:Polychlorinated Biphenyls / Q000097:blood*; D011786:Quality Control; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000191:economics / Q000379:methods*
D004147:Dioxins; D011078:Polychlorinated Biphenyls