Distinct cytokine and chemokine profiles in the aqueous of patients with uveitis and cystoid macular edema.
OBJECTIVE
To investigate the global cytokine and chemokine expression pattern in the aqueous humor of uveitis patients and relate them to clinical features.


METHODS
Cross-sectional study.


METHODS
In 31 aqueous humor samples from uveitis patients, the concentration of mediators was measured by a multiplex immunoassay. Eleven control samples were included.


RESULTS
Uveitis samples had higher levels of interleukin-6, interleukin-8, soluble intercellular adhesion molecule, soluble vascular cell adhesion molecule (sVCAM), and interferon-inducible protein-10 (IP-10) than nonuveitis controls. Active uveitis samples had higher levels of interleukin-6, interleukin-10, interferon-gamma, sVCAM, regulated on activation, normal T cell expressed and secreted (RANTES), and IP-10 than quiescent uveitis samples. Infectious uveitis was associated with higher levels of interleukin-10 than noninfectious uveitis (P < .03 for all subgroups). No significant differences were found between cystoid macular edema (CME) and non-CME samples.


CONCLUSIONS
Elevated levels of specific mediators were found in active and in infectious uveitis, but not in CME. Mediator profiles might lead to a better understanding of the pathogenesis of uveitis.
van Kooij
Bram
B
F. C. Donders Institute of Ophthalmology, Utrecht, The Netherlands. b.vankooij@oogh.azu.nl
Rothova
Aniki
A
Rijkers
Ger T
GT
de Groot-Mijnes
Jolanda D F
JD
D000328:Adult; D000368:Aged; D001082:Aqueous Humor / Q000378:metabolism*; D018925:Chemokines / Q000378:metabolism; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008269:Macular Edema / Q000378:metabolism*; D008875:Middle Aged; D014605:Uveitis / Q000378:metabolism*
D018925:Chemokines; D016207:Cytokines