ZNF325, a novel human zinc finger protein with a RBaK-like RB-binding domain, inhibits AP-1- and SRE-mediated transcriptional activity.
Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of eukaryotic cell regulation. The zinc-finger-containing transcription factors have been previously revealed to be involved in the regulation of the MAPK signaling pathways. Here, we have identified a novel human zinc-finger transcriptional repressor, ZNF325, that contains a RBaK-like RB-binding domain and 15 tandem repeated C2H2 type zinc fingers. Northern blot analysis indicates that a 2.7 kb transcript specific for ZNF325 is widely expressed in all tissues examined at adult stage and in most of the embryonic tissues. Overexpression of ZNF325 in COS-7 cells inhibits the transcriptional activities of AP-1 and SRE. The deletion and RNAi analysis indicate that the C2H2 zinc finger motifs represent the basal transcriptional repressive activity. These results indicate that the ZNF325 protein may act as a novel transcription repressor in MAPK signaling pathway to mediate cellular functions.
Zhao
Yulian
Y
The Center for Heart Development, Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, 410081 Hunan, People's Republic of China.
Zhou
Liang
L
Liu
Bisheng
B
Deng
Yun
Y
Wang
Ying
Y
Wang
Yuequn
Y
Huang
Wen
W
Yuan
Wuzhou
W
Wang
Zequn
Z
Zhu
Chuanbing
C
Liu
Mingyao
M
Wu
Xiushan
X
Li
Yongqing
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D018076:DNA, Complementary / Q000032:analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D016160:Retinoblastoma Protein / Q000378:metabolism; D027602:Serum Response Element / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000502:physiology*; D016335:Zinc Fingers
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D016160:Retinoblastoma Protein; D018808:Transcription Factor AP-1; D014157:Transcription Factors; C512444:ZNF12 protein, human; D020928:Mitogen-Activated Protein Kinases