title: Effect of smear layer and chlorhexidine treatment on the adhesion of Enterococcus faecalis to bovine dentin.
abstract: The aim of this in vitro study was to determine the effects of a smear layer and chlorhexidine (CHX) treatment on the adhesion of Enterococcus faecalis to bovine dentin. Forty dentin blocks from bovine incisors were prepared and randomly divided into four groups of 10 each. The blocks in group 1 were placed in sterile saline for 5 minutes, while those in group 2 were treated with 17% EDTA for 5 minutes. The blocks in group 3 were placed in 2% CHX for 7 days. The blocks in group 4 were treated with 17% EDTA for 5 minutes, and then placed in 2% CHX for 7 days. All the blocks were immersed in a suspension of E. faecalis for 3 hours. The bacteria adhering to the dentin surface were counted by examination using a scanning electron microscope. The most significant amount of bacteria was retained on the samples from group 1 (p < 0.05) and the smallest amount of bacteria adhered to the samples from group 4. These results suggest that a smear layer enhances the adherence of E. faecalis to the dentin, and CHX is effective in reducing the adherence of microorganisms.
authors.affiliation: Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seodaemun-gu, Seoul, Republic of Korea.
authors.forename: Seung-Eun
authors.initials: SE
authors.lastname: Yang
authors.forename: Jeong-Heon
authors.initials: JH
authors.lastname: Cha
authors.forename: Eui-Seong
authors.initials: ES
authors.lastname: Kim
authors.forename: Kee-Yeon
authors.initials: KY
authors.lastname: Kum
authors.forename: Chang-Young
authors.initials: CY
authors.lastname: Lee
authors.forename: Il-Young
authors.initials: IY
authors.lastname: Jung
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D002417:Cattle; D002710:Chlorhexidine / Q000494:pharmacology*; D003804:Dentin / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D012388:Root Canal Irrigants / Q000494:pharmacology*; D016369:Smear Layer; D018709:Statistics, Nonparametric
chemical_list: D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants; D002710:Chlorhexidine