Matrix metalloproteinase-9 regulates graft bone resorption.
OBJECTIVE
To explore the relationship between bone resorption and matrix metalloproteinase-9 (MMP-9) expression in autogenous and allogenic bone grafts.


METHODS
A total of 18 critical-size (10 x 15 mm) defects were created in rabbit mandibles bilaterally. Three groups of six defects each were grafted with autogenous endochondral (EC) bone, autogenous intramembranous (IM) bone, and allogenic IM bone. Three months later, the defects were retrieved for quantitative analysis on the basis of histology, immunohistochemistry, and in situ hybridization.


RESULTS
A close relationship existed between MMP-9 expression and graft bone resorption. The parallel between MMP-9 expression and graft bone resorption suggested that bone resorption was accomplished in part by increased MMP-9 production evident in preosteoclasts and osteoclasts.


CONCLUSIONS
MMP-9 plays an important role in graft bone resorption. Autogenous EC bone grafts express higher levels of MMP-9 leading to more resorption than autogenous or allogenic IM bone grafts.
Lu
Mei
M
Department of Oral Health Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, Canada.
Rabie
A B M
AB
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D016026:Bone Marrow Transplantation / Q000473:pathology; D001862:Bone Resorption / Q000201:enzymology*; D016025:Bone Transplantation / Q000145:classification / Q000473:pathology*; D003593:Cytoplasm / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000032:analysis; D008334:Mandible / Q000601:surgery; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000502:physiology*; D010010:Osteoclasts / Q000201:enzymology; D010503:Periodic Acid-Schiff Reaction; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D015415:Biomarkers; D007527:Isoenzymes; D012333:RNA, Messenger; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D020780:Matrix Metalloproteinase 9