title: Ozone monitoring based on a biosensor concept utilizing a reagentless alcohol oxidase electrode.
abstract: An electrochemical method based on the concept of a biosensor for the monitoring of ozone is described for first time. The proposed method includes two parts: a selective sorbent for ozone, that is, eugenol, and a formaldehyde amperometric biosensor mounted into a flow-through cell. Ozone adds rapidly to the double bond of the allyl group of eugenol, which has been immobilized onto a hydrophobic C-18 reactor and the so produced formaldehyde is collected into the working buffer solution (sampler) and pumped to the detector. A multimembrane assembly consisting of an alcohol oxidase-modified nylon membrane sandwiched between an outer polycarbonate and an inner cellulose acetate membrane was fitted onto a Pt electrode and the enzymatically produced H2O2 was monitored at +0.65 V (vs Ag/AgCl/KCl 3 M). Under optimum conditions, a linear calibration curve over the concentration range 3-200 microg x mL(-1) ozone was constructed. The detection limit (S/N = 3) was calculated at 1.1 microg x mL(-1) ozone. The proposed method is interference-free from other gases such as O2, Ar, N2, N2O, NOCl, SO2, NH3, and CO2, which were tested at concentrations >200-fold higher than that of 100 microg x mL(-1) ozone used for comparison. Besides selectivity, the method is easy to perform and reproducible; its applicability in synthetic gaseous samples is also demonstrated.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Ioannina, 45110-Ioannina, Greece.
authors.forename: Dimitrios V
authors.initials: DV
authors.lastname: Stergiou
authors.forename: Mamas I
authors.initials: MI
authors.lastname: Prodromidis
authors.forename: Panayotis G
authors.initials: PG
authors.lastname: Veltsistas
authors.forename: Nicholaos P
authors.initials: NP
authors.lastname: Evmiridis
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D015374:Biosensing Techniques; D004566:Electrodes; D010126:Ozone / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D010126:Ozone; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase