Adiponectin plays an important role in efficient energy usage under energy shortage.
Adiponectin is an adipose tissue-specific secretory protein known to be an insulin-sensitizing protein. In this study, we generated adiponectin sense and antisense transgenic (Tg) mice to investigate whether adiponectin plays a role in the regulation of energy homeostasis during the growth stage. Spontaneous motor activity of antisense Tg mice were markedly reduced during fasting, particularly in young female mice, compared with wild type (Wt) and sense Tg mice. Furthermore, both body weight and adipose tissue mass of the antisense female Tg mice drastically reduced during fasting. To examine the relationship between the collapse of abdominal white adipose tissue (WAT) and serum adiponectin level, we measured the expression of genes related to energy expenditure, such as uncoupling protein (UCP). Notably, the mRNA of UCP1 in the WAT of antisense Tg female mice was markedly less than that of Wt mice and the UCP1 mRNA was strongly increased during fasting. These findings suggest that the serum adiponectin is important to maintaining energy homeostasis under energy shortage conditions, such as over female pubertal development.
Saito
Kiyomi
K
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan. kiyomi-s@pharm.showa-u.ac.jp
Arata
Satoru
S
Hosono
Tomohiko
T
Sano
Yoshihiro
Y
Takahashi
Katsuhiko
K
Choi-Miura
Nam-Ho
NH
Nakano
Yasuko
Y
Tobe
Takashi
T
Tomita
Motowo
M
D052242:Adiponectin / Q000235:genetics / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000375:Aging / Q000502:physiology; D000818:Animals; D001835:Body Weight; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D016373:DNA, Antisense; D004734:Energy Metabolism / Q000235:genetics; D005215:Fasting; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009928:Organ Specificity; D012737:Sex Factors; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2
D052242:Adiponectin; D002352:Carrier Proteins; D016373:DNA, Antisense; D007473:Ion Channels; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2