title: Modified His-tag fusion vector for enhanced protein purification by immobilized metal affinity chromatography.
authors.affiliation: School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland.
authors.forename: Sinéad T
authors.initials: ST
authors.lastname: Loughran
authors.forename: Noeleen B
authors.initials: NB
authors.lastname: Loughran
authors.forename: Barry J
authors.initials: BJ
authors.lastname: Ryan
authors.forename: Brendan N
authors.initials: BN
authors.lastname: D'Souza
authors.forename: Dermot
authors.initials: D
authors.lastname: Walls
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D006639:Histidine / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008670:Metals / Q000737:chemistry* / Q000378:metabolism; D015778:Minor Histocompatibility Antigens; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: C096816:BCL2-related protein A1; D008670:Metals; D015778:Minor Histocompatibility Antigens; D019253:Proto-Oncogene Proteins c-bcl-2; D011993:Recombinant Fusion Proteins; D006639:Histidine