title: A low temperature bonding of quartz microfluidic chip for serum lipoproteins analysis.
abstract: A low-temperature bonding method for microfabrication of quartz microfluidic chips has been developed. The bonding process involved two steps: pre-bonding and post-annealing at low temperature. The bonding quality was evaluated by measuring the shear force at bonding interface and the electrical properties of the chips. Shear force of 5.66 MPa (566 N/cm(2)) was obtained in a bonded chip after a post-annealing at 200 degrees C for 6 h. We owe the strong bonding strength to the formation of Si-O-Si bonds at the bonding interface during the post-annealing stage. The bonding procedures were not sensitive to surrounding and could be performed in a routine laboratory without clean room conditions. The performance of the fabricated microfluidic chips was tested by capillary zone electrophoresis (CZE) of serum lipoproteins with laser-induced fluorescence (LIF). The low-density (LDL) and high-density (HDL) lipoproteins in the serum was separated completely by using tricine buffer with methylglucamine.
authors.affiliation: Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, 865 Chang Ning Road, 200050 Shanghai, China. guishengzhuang@gmail.com
authors.forename: Guisheng
authors.initials: G
authors.lastname: Zhuang
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Jin
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Cong
authors.forename: Kangdong
authors.initials: K
authors.lastname: Liu
authors.forename: Jianlong
authors.initials: J
authors.lastname: Zhao
authors.forename: Mengsu
authors.initials: M
authors.lastname: Yang
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
mesh_terms: D003080:Cold Temperature; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D011791:Quartz / Q000737:chemistry
chemical_list: D008074:Lipoproteins; D011791:Quartz