Bradykinin-related peptides from Phyllomedusa hypochondrialis.
Bradykinin related peptides (BRPs) present in the water-soluble secretion and freshly dissected skin fragments of Phyllomedusa hypochondrialis were investigated by mass spectrometry techniques. Eighteen BRPs, along with their post-translational modifications, were characterized in the secretion by de novo MS/MS sequencing and direct MALDI imaging experiments of the frog skin. These molecules revealed strong sequence similarities to the main plasma kinin of some mammals and reptiles. Such a diversity of molecules, within the same peptide family, belonging to a single amphibian species may be related to functional specializations of these peptides and a variety of corresponding receptors that might be present in a number of different predators. Also, a novel analog, [Val]1,[Thr]6-bradykinyl-Gln,Ser had its biological activity positively detected in cell culture expressing the human bradykinin B2 receptor and in guinea pig ileum preparations.
Brand
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GD
Laboratório de Espectrometria de Massa, EMBRAPA, Recursos Genéticos e Biotecnologia, Brasília, DF 70770-900, Brazil.
Krause
F C
FC
Silva
L P
LP
Leite
J R S A
JR
Melo
J A T
JA
Prates
M V
MV
Pesquero
J B
JB
Santos
E L
EL
Nakaie
C R
CR
Costa-Neto
C M
CM
Bloch
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000737:chemistry*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D006909:Hydroxyproline / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism; D011898:Ranidae / Q000145:classification / Q000378:metabolism*; D043782:Receptor, Bradykinin B2 / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection
D043782:Receptor, Bradykinin B2; D006909:Hydroxyproline; D001920:Bradykinin