title: Efficiency of three surgical procedures in eliminating high-risk human papillomavirus infection in women with precancerous cervical lesions.
abstract: OBJECTIVE
  To establish the efficiency of laser vaporization (LV), large loop excision of the transformation zone (LLETZ) and cold knife conization, done for precancerous cervical lesions, in eliminating high-risk human papillomavirus (HPV) infection. Additionally, we determined whether the same HPV genotype persisted after surgery.
  
  
  METHODS
  A total of 214 women were tested for HPV infection by the Hybrid Capture II (HCII) test prior to surgery. HPV-positive women were followed by HCII test ten months after surgery. In persistently HPV-positive women, HPV genotypes were determined by PCR - PGMY09/PGMY11.
  
  
  RESULTS
  The HCII test showed elimination of HPV infection after LV, LLETZ and cold knife conization in 67.6%, 86.3%, and 100% (p < 0.05) of women, respectively. In seven (38.9%) women a different HPV genotype was found to be present after surgery, the corrected efficiency thus being 79.4%, 92.7% and 100% (p = NS), respectively.
  
  
  CONCLUSIONS
  The three analyzed surgical procedures are effective in eliminating high-risk HPV infection. HPV testing is useful at follow-up, since it can identify a small proportion of women requiring close surveillance and potential treatment.
authors.affiliation: Department of Obstetrics and Gynecology, University Medical Centre Ljubljana, Ljubljana, Slovenia.
authors.forename: N
authors.initials: N
authors.lastname: Jancar
authors.forename: S
authors.initials: S
authors.lastname: Rakar
authors.forename: M
authors.initials: M
authors.lastname: Poljak
authors.forename: K
authors.initials: K
authors.lastname: Fujs
authors.forename: B J
authors.initials: BJ
authors.lastname: Kocjan
authors.forename: E
authors.initials: E
authors.lastname: Vrtacnik-Bokal
mesh_terms: D000328:Adult; D019092:Conization; D005260:Female; D005838:Genotype; D006801:Humans; D053685:Laser Therapy; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000150:complications / Q000601:surgery*; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000601:surgery / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000601:surgery* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000601:surgery* / Q000821:virology