title: High-resolution image reconstruction in fluorescence microscopy with patterned excitation.
abstract: We discuss data treatment strategies in structured illumination microscopy, using simulated and experimental data. In the setup, the illumination pattern is generated by projecting a movable pinhole mask into the sample, and a wide-field fluorescence microscope image is acquired for each pattern position. The structured illumination data obtained from a two-dimensional illumination pattern can be treated by projection strategies such as in video confocal microscopy (sum, maximum, maximum minus minimum, and superconfocal), by a scaled subtraction of the out-of-focus estimate, or by a modified version of the Fourier-space treatment, as is known for data from one-dimensional structured illumination. We investigate the influence of some data processing strategies on unwanted effects such as residual patterning and local deviations from linearity in the reconstructed intensity.
authors.affiliation: Randall Division of Cell and Molecular Biophysics, Kings College London, London SE1 1UL, UK. rainer.heintzmann@kcl.ac.uk
authors.forename: Rainer
authors.initials: R
authors.lastname: Heintzmann
authors.forename: Pier A
authors.initials: PA
authors.lastname: Benedetti
mesh_terms: D000465:Algorithms; D016477:Artifacts; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity