title: [Transplantation of autologous adult bone marrow stem cells in patients with severe peripheral arterial occlusion disease].
abstract: BACKGROUND
  For many patients with severe peripheral arterial occlusion disease (PAOD) an interventional or surgical treatment is not feasible. The regenerative potential of adult autologous mononuclear stem cells could contribute to neoangiogenesis.
  
  
  METHODS
  Ten patients with severe PAOD were included. The walking distance was < 200 m and no interventional or surgical treatment was possible. After harvesting of 80 ml of bone marrow the mononuclear cell fraction was separated. Thereafter, intraarterial (10 ml into the common femoral artery) and intramuscular (5 ml into the muscles of the thigh and the lower leg) transplantation of the cell suspension was performed.
  
  
  RESULTS
  After 2 months the walking distance was enhanced significantly in all patients. Furthermore, a significant improvement of ankle-brachial index at rest, capillary-venous oxygen saturation and parameters of venous occlusion plethysmography was seen. No complications or side effects could be monitored.
  
  
  CONCLUSIONS
  These results demonstrate, that the combined intraarterial and intramuscular transplantation of autologous adult bone marrow stem cells leads to a significant improvement of perfusion indices in patients with severe PAOD.
authors.affiliation: Klinik für Kardiologie, Pneumologie und Angiologie, Heinrich-Heine-Universität Düsseldorf.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bartsch
authors.forename: Till
authors.initials: T
authors.lastname: Falke
authors.forename: Michael
authors.initials: M
authors.lastname: Brehm
authors.forename: Tobias
authors.initials: T
authors.lastname: Zeus
authors.forename: Gesine
authors.initials: G
authors.lastname: Kögler
authors.forename: Peter
authors.initials: P
authors.lastname: Wernet
authors.forename: Bodo-Eckehard
authors.initials: BE
authors.lastname: Strauer
mesh_terms: D000368:Aged; D001157:Arterial Occlusive Diseases / Q000628:therapy*; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation / Q000502:physiology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007269:Injections, Intra-Arterial; D007273:Injections, Intramuscular; D007511:Ischemia / Q000628:therapy*; D007866:Leg / Q000098:blood supply*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010100:Oxygen / Q000097:blood; D016896:Treatment Outcome; D016138:Walking / Q000502:physiology
chemical_list: D010100:Oxygen