Recombinant kallikrein expression: site-specific integration for hK6 production in human cells.
Kallikreins have been implicated in carcinogenesis and are promising biomarkers for the diagnosis and follow-up of various cancers. To evaluate the functions and clinical interest of kallikreins, it is important to be able to produce them as recombinant proteins. Here we summarize the various strategies used to produce kallikreins, emphasizing their advantages and limitations. We also describe an approach to achieve high-level production of kallikreins, such as hK6, with correct folding and activity, combining an expression system with targeted transgene integration and an efficient cultivation device to increase yield, the CELLine bioreactor. This novel method for recombinant kallikrein production will be useful to study their bio-pathological functions and to develop anti-bodies.
Heuzé-Vourc'h
Nathalie
N
INSERM, U618 Protéases et Vectorisation Pulmonaires, F-37032 Tours, France. heuze_vourch@yahoo.fr
Aïnciburu
Mireille
M
Planque
Chris
C
Brillard-Bourdet
Michèle
M
Ott
Catherine
C
Jolivet-Reynaud
Colette
C
Courty
Yves
Y
D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D006801:Humans; D007610:Kallikreins / Q000235:genetics*; D011994:Recombinant Proteins; D011995:Recombination, Genetic
D011994:Recombinant Proteins; C103551:KLK6 protein, human; D007610:Kallikreins