Hyaluronan in human acute and chronic dermal wounds.
There is growing interest in the relationship of hyaluronan and inflammation in a number of physiologic processes including wound healing. The objective of this study was to make a quantitative comparison of inflammation and hyaluronan expression in human normal healing open wounds and in pressure ulcers. Using an open dermal wound model, myeloperoxidase activity was found to peak at day 3. Hyaluronan levels showed a bimodal distribution with transient peaks occurring on days 1 and 7. Mean levels of myeloperoxidase activity in pressure ulcers were significantly higher than at any time in the acute wounds, whereas hyaluronan levels were significantly lower than at any time in the acute wounds. Levels of hyaluronidase activity increased slightly in the postwound period. Hyaluronidase activity in pressure ulcers was significantly elevated compared with the acute wounds. These results suggest a role for increased enzymatic degradation of hyaluronan as a function of inflammation during wound repair. This is the first reported quantitative examination of hyaluronan expression in human acute dermal wounds and in chronic pressure ulcers.
Dechert
Tracey A
TA
Department of Surgery, Virginia Commonwealth University Medical Center, Richmond, Virginia 23298, USA.
Ducale
Ashley E
AE
Ward
Susan I
SI
Yager
Dorne R
DR
D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D066298:In Vitro Techniques; D007249:Inflammation; D008970:Molecular Weight; D009195:Peroxidase / Q000378:metabolism; D003668:Pressure Ulcer / Q000378:metabolism; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing
D006820:Hyaluronic Acid; D009195:Peroxidase; D006821:Hyaluronoglucosaminidase