Single-molecule detection sensitivity using planar integrated optics on a chip.
We present a fully planar integrated optical approach to single-molecule detection based on microfabricated planar networks of intersecting solid and liquid-core waveguides. We study fluorescence from dye molecules in liquid-core antiresonant reflecting optical waveguides, and demonstrate subpicoliter excitation volumes, parallel excitation through multiple pump waveguides, and single-molecule detection sensitivity. Integrated silicon photonics combined with single-molecule detection in solution create a compact, robust, and sensitive platform that has applications in numerous fields ranging from atomic physics to the life sciences.
Yin
Dongliang
D
School of Engineering, University of California Santa Cruz 95064, USA.
Deamer
David W
DW
Schmidt
Holger
H
Barber
John P
JP
Hawkins
Aaron R
AR
D001704:Biopolymers / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D056656:Lab-On-A-Chip Devices; D046208:Microchip Analytical Procedures / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D055095:Optics and Photonics / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018511:Systems Integration
D001704:Biopolymers