Free-radical scavenging by Ouratea parviflora in experimentally-induced liver injuries.
The antioxidant potential of crude extracts and fractions from leaves of Ouratea parviflora, a Brazilian medicinal plant used for the treatment of inflammatory diseases, was investigated in vitro through the scavenging of radicals 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), hydroxyl radical (HO*), superoxide anion (O2*-), and lipid peroxidation in rat liver homogenate. The crude extract (CEOP) and hydro-alcoholic fraction (OP4) showed strong inhibitory activity toward lipid peroxidation induced by tert-butyl peroxide (IC50 = 2.3 +/- 0.2 and 1.9 +/- 0.1 microg/ml, respectively). The same products exhibited a strong concentration-dependent inhibition of deoxyribose oxidation (14.9 +/- 0.2 and 0.2 +/- 0.1 microg/ml, respectively), and also showed a considerable antioxidant activity against O2*- (87.3 +/- 0.1 and 73.1 +/- 0.4 microg/ml, respectively) and DPPH radicals (55.4 +/- 0.3 and 38.3 +/- 0.4 microg/ml, respectively). The protective effects of CEOP and OP4 were also studied in mouse liver. CCl4 significantly increased (by 90%) levels of lipid hydroperoxides, carbonyl protein content (64%), DNA damage index (133%), aspartate aminotransferase (261%), alanine aminotransferase (212%), catalase activity (23%), and also caused a decrease of 60% in GSH content. The results showed that CEOP and OP4 exerted cytoprotective effects against oxidative injury caused by CCl4 in rat liver, probably related to the antioxidant activity showed by the in vitro free radical scavenging property.
Carbonari
Karina Azambuja
KA
Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Ferreira
Eduardo Antonio
EA
Rebello
Jussara Mattos
JM
Felipe
Karina Bettega
KB
Rossi
Maria Helena
MH
Felício
Joana D'arc
JD
Filho
Danilo Wilhelm
DW
Yunes
Rosendo Augusto
RA
Pedrosa
Rozangela Curi
RC
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000378:metabolism*; D005609:Free Radicals; D008099:Liver / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D043842:Ochnaceae / Q000378:metabolism*; D018384:Oxidative Stress; D010936:Plant Extracts / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013481:Superoxides