Sendai virus infection up-regulates trypsin I and matrix metalloproteinase-9, triggering viral multiplication and matrix degradation in rat lungs and lung L2 cells.
To elucidate the virus-host cell interaction, we analyzed quantitatively the expression of various cellular proteases and tumor necrosis factor-alpha (TNF-alpha) after Sendai virus infection in rat lungs and lung L2 cells. After infection, TNF-alpha mRNA levels increased rapidly to a peak on day one, and then trypsin I and matrix metalloproteinase (MMP)-9, but not MMP-2, were significantly up-regulated with a peak on day 2 in vivo. These up-regulations were confirmed in L2 cells. Up-regulation of proMMP-9 and its active convertase trypsin I seems to synergistically enhance virus multiplication and the destruction of lung matrix, resulting in the progression of pneumonia.
Yamada
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Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Tokushima, Japan.
Le
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QT
Kousaka
A
A
Higashi
Y
Y
Tsukane
M
M
Kido
H
H
D000818:Animals; D002460:Cell Line; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D007700:Kinetics; D008168:Lung / Q000821:virology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D029082:Sendai virus / Q000472:pathogenicity / Q000502:physiology*; D014357:Trypsin / Q000235:genetics*; D014779:Virus Replication
D017931:DNA Primers; D014357:Trypsin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9