title: Production of an antimicrobial substance against Cryptococcus neoformans by Paenibacillus brasilensis Sa3 isolated from the rhizosphere of Kalanchoe brasiliensis.
abstract: An antifungal substance produced by Paenibacillus brasilensis strain Sa3 was preliminary characterized and showed to be stable after treatment with different enzymes and organic solvents and at a wide range of pH, and presented a molecular weight between 3 and 10 kDa. In vitro antagonism of this strain towards Cryptococcus neoformans was investigated by optical and electronic microscopic analyses and a fungicidal effect on C. neoformans was observed. Ultrastructural analysis showed intense changes on the fungus when it was paired cultured with strain Sa3, mainly the detachment of the capsule from the cell wall and the presence of altered organelles in the cytoplasm. This novel antifungal substance produced by P. brasilensis Sa3 may represent a new insight in antifungal therapy mainly against emergent fungi. Also, prospective studies on rhizobacteria of plants as Kalanchoe brasiliensis may offer a potential source for the discovery of bioactive compounds with medical value.
authors.affiliation: Laboratório de Genética Microbiana, Centro de Ciências da Saúde, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Bloco I, Ilha do Fundão, CEP 21941-590 Rio de Janeiro, RJ, Brasil.
authors.forename: Tiago Oliveira
authors.initials: TO
authors.lastname: Fortes
authors.forename: Daniela Sales
authors.initials: DS
authors.lastname: Alviano
authors.forename: Gleiser
authors.initials: G
authors.lastname: Tupinambá
authors.forename: Thaís Souto
authors.initials: TS
authors.lastname: Padrón
authors.forename: Angelo Roberto
authors.initials: AR
authors.lastname: Antoniolli
authors.forename: Celuta Sales
authors.initials: CS
authors.lastname: Alviano
authors.forename: Lucy
authors.initials: L
authors.lastname: Seldin
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification; D001407:Bacillus / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D001938:Brazil; D003453:Cryptococcosis / Q000382:microbiology; D003455:Cryptococcus neoformans / Q000254:growth & development* / Q000648:ultrastructure; D005658:Fungi / Q000254:growth & development; D006801:Humans; D031962:Kalanchoe / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D018517:Plant Roots / Q000382:microbiology
chemical_list: D000935:Antifungal Agents