Immunohistochemical study of caveolin-1 in the sciatic nerves of Lewis rats with experimental autoimmune neuritis.
The expression of caveolin-1 and the related molecule endothelial nitric oxide synthase (eNOS) was analyzed in the sciatic nerves of Lewis rats with experimental autoimmune neuritis (EAN). Western blot analysis showed that caveolin-1 significantly increased in the sciatic nerves with EAN upon initiation of cell infiltration during the early and peak stages (days 10 and 14 post-immunization, p.i.) and declined thereafter. The pattern of eNOS expression over the course of EAN largely matched that of caveolin-1. Immunohistochemistry showed that in EAN lesions, intense caveolin-1 immunostaining occurred in ED1-positive macrophages as well as in vessels, while the caveolin-1 immunoreaction was reduced in Schwann cells in the inflammatory lesions. Consequently, we postulated that caveolin-1 expression increased in the sciatic nerves with EAN; this possibly mediated either molecular trafficking or nitric oxide generation partly through the activation of eNOS in vascular endothelial cells, as well as in inflammatory macrophages in EAN and/or cellular apoptosis of inflammatory cells.
Ahn
Meejung
M
Department of Veterinary Medicine, Cheju National University, Jeju, South Korea.
Moon
Changjong
C
Kim
Heechul
H
Lee
Jeeyoung
J
Sung Koh
Chang
C
Matsumoto
Yoh
Y
Shin
Taekyun
T
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D051242:Caveolin 1 / Q000378:metabolism*; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000502:physiology; D004195:Disease Models, Animal; D053331:Ectodysplasins; D005786:Gene Expression Regulation / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling / Q000379:methods; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009444:Neuritis, Autoimmune, Experimental / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D012584:Sciatic Nerve / Q000378:metabolism*; D013997:Time Factors; D048069:Tumor Necrosis Factors / Q000378:metabolism
D051242:Caveolin 1; D053331:Ectodysplasins; D008565:Membrane Proteins; D048069:Tumor Necrosis Factors; D010751:Phosphopyruvate Hydratase