title: Odd-skipped related 1 is required for development of the metanephric kidney and regulates formation and differentiation of kidney precursor cells.
abstract: Formation of kidney tissue requires the generation of kidney precursor cells and their subsequent differentiation into nephrons, the functional filtration unit of the kidney. Here we report that the gene odd-skipped related 1 (Odd1) plays an important role in both these processes. Odd1 is the earliest known marker of the intermediate mesoderm, the precursor to all kidney tissue. It is localized to mesenchymal precursors within the mesonephric and metanephric kidney and is subsequently downregulated upon tubule differentiation. Mice lacking Odd1 do not form metanephric mesenchyme, and do not express several other factors required for metanephric kidney formation, including Eya1, Six2, Pax2, Sall1 and Gdnf. In transient ectopic expression experiments in the chick embryo, Odd1 can promote expression of the mesonephric precursor markers Pax2 and Lim1. Finally, persistent expression of Odd1 in chick mesonephric precursor cells inhibits differentiation of these precursors into kidney tubules. These data indicate that Odd1 plays an important role in establishing kidney precursor cells, and in regulating their differentiation into kidney tubular tissue.
authors.affiliation: Molecular and Vascular Medicine Unit, Beth Israel Deaconess Medical Center, 330 Brookline Ave, RW-663, Boston, MA 02215, USA.
authors.forename: Richard G
authors.initials: RG
authors.lastname: James
authors.forename: Caramai N
authors.initials: CN
authors.lastname: Kamei
authors.forename: Qingru
authors.initials: Q
authors.lastname: Wang
authors.forename: Rulang
authors.initials: R
authors.lastname: Jiang
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Schultheiss
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D018507:Gene Expression Regulation, Developmental; D007668:Kidney / Q000166:cytology / Q000196:embryology*; D007684:Kidney Tubules / Q000196:embryology; D008648:Mesoderm / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics*
chemical_list: C121641:Osr1 protein, mouse; D014157:Transcription Factors