SFTG international collaborative study on in vitro micronucleus test I. General conditions and overall conclusions of the study.
This study, coordinated by the SFTG (French branch of European Environmental Mutagen Society), included 38 participants from Europe, Japan and America. Clastogens (bleomycin, urethane), including base and nucleoside analogs (5-fluorouracil and cytosine arabinoside), aneugens and/or polyploidy inducers (colchicine, diethylstilboestrol, griseofulvin and thiabendazole), as well as non-genotoxic compounds (mannitol and clofibrate), were tested. Four cell types were used, i.e. human lymphocytes in the presence of cytochalasin B and CHO, CHL and L5178Y cell lines, in the presence or absence of cytochalasin B, with various treatment-recovery schedules. Mitomycin C was used as a positive control for all cell types. Mannitol and clofibrate were consistently negative in all cell types and with all treatment-recovery conditions. Urethane, known to induce questionable clastogenicity, was not found as positive. Bleomycin and mitomycin C were found positive in all treatment-recovery conditions. The base and nucleoside analogs were less easy to detect, especially 5-fluorouracil due to the interference with cytotoxicity, while cytosine arabinoside was detected in all cell types depending on the treatment-recovery schedule. Aneugens (colchicine, diethylstilboestrol and griseofulvin) were all detected in all cell types. In this study, the optimal detection was ensured when a short treatment followed by a long recovery was associated with a long continuous treatment without recovery. There was no impact of the presence or absence of cytochalasin B on the detection of micronucleated cells on cell lines. Scoring micronucleated cells in both mononucleated and binucleated cells when using cytochalasin B was confirmed to be useful for the detection and the identification of aneugens. In conclusion, these results, together with previously published validation studies, provide a useful contribution to the optimisation of a study protocol for the detection of both clastogens and aneugens in the in vitro micronucleus test.
Lorge
Elisabeth
E
Servier Group, Drug Safety Assessment, Orleans-Gidy, France. elisabeth.lorge@fr.netgrs.com
Thybaud
Veronique
V
Aardema
Marilyn J
MJ
Oliver
Jo
J
Wakata
Akihiro
A
Lorenzon
Giocondo
G
Marzin
Daniel
D
D024422:Aneugens / Q000633:toxicity; D000818:Animals; D001761:Bleomycin / Q000633:toxicity; D016466:CHO Cells; D002460:Cell Line; D002994:Clofibrate / Q000633:toxicity; D003078:Colchicine / Q000633:toxicity; D006224:Cricetinae; D003561:Cytarabine / Q000633:toxicity; D003571:Cytochalasin B; D004054:Diethylstilbestrol / Q000633:toxicity; D005472:Fluorouracil / Q000633:toxicity; D006118:Griseofulvin / Q000633:toxicity; D006801:Humans; D066298:In Vitro Techniques; D007391:International Cooperation; D007940:Leukemia L5178; D008214:Lymphocytes / Q000187:drug effects; D008353:Mannitol / Q000633:toxicity; D051379:Mice; D015162:Micronucleus Tests / Q000379:methods* / Q000592:standards; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity; D013827:Thiabendazole / Q000633:toxicity
D024422:Aneugens; D009153:Mutagens; D003561:Cytarabine; D001761:Bleomycin; D006118:Griseofulvin; D003571:Cytochalasin B; D008353:Mannitol; D016685:Mitomycin; D004054:Diethylstilbestrol; D002994:Clofibrate; D013827:Thiabendazole; D003078:Colchicine; D005472:Fluorouracil