title: Characterization of arylsulfatase formed by derepressed synthesis in Citrobacter braakii.
abstract: Arylsulfatase activity was detected in a bacterial strain, Citrobacter braakii 69-b, isolated from soil by enrichment cultivation using porcine gastric mucin. The production of arylsulfatase was derepressed markedly in a synthetic medium by the addition of tyramine. The purified enzyme hydrolyzed 4-nitrophenyl sulfate, 4-nitrocatechol sulfate, and 3-indoxyl sulfate, and was classified as type I arylsulfatase.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Tottori University.
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Miura
authors.forename: Kenji
authors.initials: K
authors.lastname: Okamoto
authors.forename: Hideshi
authors.initials: H
authors.lastname: Yanase
mesh_terms: D001192:Arylsulfatases / Q000096:biosynthesis / Q000378:metabolism*; D002954:Citrobacter / Q000201:enzymology*; D013379:Substrate Specificity; D014439:Tyramine / Q000378:metabolism
chemical_list: D001192:Arylsulfatases; D014439:Tyramine