title: Association of uncultivated oral phylotypes AU126 and X112 with periodontitis.
abstract: OBJECTIVE
  To discuss the relationship between uncultivated pathogenic bacteria and periodontitis.
  
  
  METHODS
  Specific polymerase chain reaction (PCR) primers were designed for phylotypes AU126 and X112; PCRs were applied to determine the prevalence of these phylotypes in 35 patients with chronic periodontitis, 26 patients with plaque-induced gingivitis and 20 healthy control subjects.
  
  
  RESULTS
  The specificity of each primer is validated on the basis of the results from sequence analysis of PCR products. AU126 and X112 were detected in the subgingival plaque samples in all the three groups. The prevalence of AU126 in subgingival plaque in chronic periodontitis (77.1%) and plaque-induced gingivitis (61.5%) is relatively higher than that in the healthy subjects (10.0%), and the difference is statistically significant (P < 0.01). The prevalence of X112 in subgingival plaque in periodontitis patients (85.7%) is higher than that in healthy subjects (30.0%), the difference (P < 0.01) being equally statistically significant. The difference between the chronic periodontitis group and the plaque-induced gingivitis group (50.0%) is statistically significant (P < 0.05).
  
  
  CONCLUSIONS
  It might be assumed that the novel uncultivated AU126 phylotype could possibly be related to chronic periodontitis and plaque-induced gingivitis, and that X112 might play a role in the progress of lesion from gingivitis to periodontitis.
authors.affiliation: Department of Oral Medicine, School of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: C L
authors.initials: CL
authors.lastname: Li
authors.forename: J P
authors.initials: JP
authors.lastname: Liang
authors.forename: Y T
authors.initials: YT
authors.lastname: Jiang
mesh_terms: D015373:Bacterial Typing Techniques; D016022:Case-Control Studies; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology; D005891:Gingivitis / Q000382:microbiology; D006801:Humans; D010518:Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S