Altered beta-amyloid precursor protein isoforms in Mexican Alzheimer's Disease patients.
OBJECTIVE
To determine the beta-amyloid precursor protein (betaAPP) isoforms ratio as a risk factor for Alzheimer's Disease and to assess its relationship with demographic and genetic variables of the disease.


METHODS
Blood samples from 26 patients fulfilling NINCDS-ADRDA diagnostic criteria for AD and 46 healthy control subjects were collected for Western blotting for betaAPP. A ratio of betaAPP isoforms, in optical densities, between the upper band (130 Kd) and the lower bands (106-110 Kd) was obtained. Odds ratios were obtained to determine risk factor of this component.


RESULTS
betaAPP ratio on AD subjects was lower than that of control subjects: 0.3662 +/- 0.1891 vs. 0.6769 +/- 0.1021 (mean +/- SD, p<0.05). A low betaAPP ratio (<0.6) showed an OR of 4.63 (95% CI 1.45-15.33). When onset of disease was taken into account, a betaAPP ratio on EOAD subjects of 0.3965 +/- 0.1916 was found vs. 0.3445 +/- 0.1965 on LOAD subjects (p>0.05).


CONCLUSIONS
Altered betaAPP isoforms is a high risk factor for Alzheimer's disease, although it has no influence on the time of onset of the disease.
Sánchez-González
V J
VJ
Laboratorio de Desarrollo-Envejecimiento, Enfermedades Neurodegenerativas, División de Neurociencias, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México. vjzahiiir@yahoo.com
Ortiz
G G
GG
Gallegos-Arreola
P
P
Macías-Islas
M A
MA
Arias-Merino
E D
ED
Loera-Castañeda
V
V
Martínez-Cano
E
E
Velázquez-Brizuela
I E
IE
Rosales-Corral
S A
SA
Curiel-Ortega
C R
CR
Pacheco-Moisés
F
F
García
J J
JJ
D000368:Aged; D000483:Alleles; D000544:Alzheimer Disease / Q000175:diagnosis*; D016229:Amyloid beta-Peptides / Q000097:blood; D016564:Amyloid beta-Protein Precursor / Q000097:blood*; D001057:Apolipoproteins E / Q000235:genetics; D015153:Blotting, Western; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020033:Protein Isoforms / Q000097:blood
D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D001057:Apolipoproteins E; D020033:Protein Isoforms