Effect of low storage temperature on some of the flavour precursors in garlic (Allium sativum).
Garlic (Allium sativum) cloves were stored at ambient temperature and 4 degrees C for periods up to six months to establish the effect of position of the individual clove within the bulb and of low storage temperature on the composition of several flavours precursors and other organic sulphur compounds, measured by gradient High Pressure Liquid Chromatography. Levels of alliin, gamma glutamyl allyl cysteine sulphoxide and gamma glutamyl isoallyl cysteine sulphoxide were statistically significantly higher in outer than in inner cloves. There was no statistically significant change in levels of alliin, the major flavour precursor, in cloves stored at 4 degrees C, remaining in the average range 17.5+/-3.8-39.1+/-7.5 mM. However, isoalliin increased significantly during storage at 4 degrees C, rising from an average 0.6+/-0.2 mM (outer cloves) -- 0.7+/-0.4 mM (inner cloves) to 7.1+/-1.7 mM (outer cloves) -- 4.1+/-0.7 mM (inner cloves). A decline in other sulphur-containing compounds, most likely to be the peptides gamma-glutamyl allylcysteine sulphoxide and gamma-glutamyl isoallylcysteine sulphoxide, occurred at the same time and possibly contributed to the increase in the flavour precursor compounds. The degree of chemical changes during storage will be of interest to the food and pharmaceutical industries.
Hughes
Jill
J
School of Biological Sciences, The Bioscience Building, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK.
Collin
Hamish A
HA
Tregova
Angela
A
Tomsett
A Brian
AB
Cosstick
Richard
R
Jones
Meriel G
MG
D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives / Q000032:analysis; D005511:Food Handling / Q000379:methods*; D005519:Food Preservation / Q000379:methods*; D005737:Garlic / Q000737:chemistry* / Q000592:standards; D006801:Humans; D013457:Sulfur Compounds / Q000032:analysis; D013649:Taste; D013696:Temperature; D013997:Time Factors
D013457:Sulfur Compounds; C006453:alliin; D003545:Cysteine