Osmotic upshift transiently inhibits uptake via ABC transporters in gram-negative bacteria.
ATP-binding cassette transporters from several rhizobia and Salmonella enterica serovar Typhimurium, but not secondarily coupled systems, were inhibited by high concentrations (100 to 500 mM) of various osmolytes, an effect reversed by the removal of the osmolyte. ABC systems were also inactivated in isolated pea bacteroids, probably due to the obligatory use of high-osmolarity isolation media. Measurement of nutrient cycling in isolated pea bacteroids is impeded by this effect.
Fox
M A
MA
School of Biological Sciences, University of Reading, Whiteknights, P.O. Box 228, Reading RG6 6AJ, United Kingdom. p.s.poole@reading.ac.uk.
White
J P
JP
Hosie
A H F
AH
Lodwig
E M
EM
Poole
P S
PS
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism*; D001692:Biological Transport; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D007700:Kinetics; D012486:Salmonella typhimurium / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D000621:Aminoisobutyric Acids; C100049:2-aminoisobutyric acid