Novel object recognition in Apoe(-/-) mice improved by neonatal implantation of wild-type multipotential stromal cells.
Multipotential bone marrow stromal cells (MSCs) from wild-type (Wt) or apolipoprotein E deficient (Apoe(-/-)) mice were implanted into the cerebral ventricles of Apoe(-/-) mice. MSCs from Wt mice continued expressing apoE up to 6 months after implantation and were associated with enhanced novel object recognition and increased microtubule-associated protein 2 (MAP2) immunoreactivity in the dentate gyrus. These data show that MSCs can be used to distinguish developmental from post-developmental effects of a gene knockout and support their therapeutic potential for neurodegenerative diseases.
Peister
Alexandra
A
Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA.
Zeitouni
Suzanne
S
Pfankuch
Timothy
T
Reger
Roxanne L
RL
Prockop
Darwin J
DJ
Raber
Jacob
J
D000818:Animals; D000831:Animals, Newborn; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods; D002552:Cerebral Ventricles / Q000378:metabolism / Q000601:surgery; D018891:Dentate Gyrus / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D039901:Totipotent Stem Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*
D001057:Apolipoproteins E; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse