title: Clinical, histopathological and immunohistochemical findings in a case of megakaryoblastic leukemia in a dog.
abstract: The clinical, hematological, and histopathologic features of megakaryoblastic leukemia (M7) were investigated in a 10-year-old female Shih-Tzu dog. Megakaryoblastic leukemia was diagnosed using anti-human platelet glycoprotein (GP IIIa) and anti-human von Willebrand factor (vWF) antibodies. The expression of CD antigen on megakaryoblasts was also assessed using a CD79a monoclonal antibody. Immunological markers allowed visualization of neoplastic megakaryocytes. Antibodies against platelet GP IIIa were demonstrated to be the most useful for the diagnosis of megakaryoblastic leukemia of paraffin-embedded canine tissues. Hematological and histological data coupled with immunohistochemical reactivity for platelet GP IIIa, vWF, and CD79a antigen in blast cells confirmed a diagnosis of M7 megakaryoblastic leukemia.
authors.affiliation: Department of Veterinary Pathology, Small Animal Tumor Diagnostic Center, College of Veterinary Medicine, Konkuk University, Seoul, KwangJin-Ku 143-701, Korea.
authors.forename: Hee-Myung
authors.initials: HM
authors.lastname: Park
authors.forename: Alan R
authors.initials: AR
authors.lastname: Doster
authors.forename: Roksana E
authors.initials: RE
authors.lastname: Tashbaeva
authors.forename: Young-Mee
authors.initials: YM
authors.lastname: Lee
authors.forename: Young S
authors.initials: YS
authors.lastname: Lyoo
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jung-Hyang
authors.initials: JH
authors.lastname: Sur
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D051925:CD79 Antigens / Q000378:metabolism; D004283:Dog Diseases / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D017809:Fatal Outcome; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D039661:Integrin beta3 / Q000378:metabolism; D007947:Leukemia, Megakaryoblastic, Acute / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D008099:Liver / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D013154:Spleen / Q000473:pathology; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D051925:CD79 Antigens; D039661:Integrin beta3; D014841:von Willebrand Factor