Key adhesin gene in community-acquired methicillin-resistant Staphylococcus aureus.
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) possessing the Panton-Valentine leukocidin (PVL) gene (luk(PV)) is associated with skin and soft tissue infections, osteomyelitis, and necrotizing pneumonia. There are geographically two types of CA-MRSA: one (sequence type ST30) that is worldwide (pandemic) and the other (sequence types, e.g., ST1, ST8 or ST80) that is continent-specific. The pandemic type, but not continent-specific type, possessed the bone sialoprotein-adhesin gene (bbp), which was associated with osteomyelitis. No recent hospital-acquired MRSA had the bbp gene, while past PVL-positive nosocomial outbreak-derived strains did possess it. The collagen-adhesin gene (cna) was associated with pandemic CA-MRSA, though with positive cases even in continent-specific CA-MRSA and PVL-negative Japanese region-specific CA-MRSA. Thus, the pandemic type is characterized by the combination of luk(PV) and bbp (and cna) genes. A specific real-time PCR assay for the bbp gene was developed, and dual assay for bbp and luk(PV) in one test tube became possible.
Otsuka
Taketo
T
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Saito
Kohei
K
Dohmae
Soshi
S
Takano
Tomomi
T
Higuchi
Wataru
W
Takizawa
Yoko
Y
Okubo
Takeshi
T
Iwakura
Nobuhiro
N
Yamamoto
Tatsuo
T
D018829:Adhesins, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D017714:Community-Acquired Infections / Q000382:microbiology*; D005098:Exotoxins / Q000235:genetics / Q000378:metabolism; D007956:Leukocidins; D016106:Methicillin Resistance / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism*
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002352:Carrier Proteins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; C405490:bone sialoprotein-binding protein, Staphylococcus aureus