Transpositions and translocations induced by site-specific double-strand breaks in budding yeast.
Much of what we know about the molecular mechanisms of repairing a broken chromosome has come from the analysis of site-specific double-strand breaks (DSBs). Such DSBs can be generated by conditional expression of meganucleases such as HO or I-SceI or by the excision of a DNA transposable element. The synchronous creation of DSBs in nearly all cells of the population has made it possible to observe the progress of recombination by monitoring both the DNA itself and proteins that become associated with the recombining DNA. Both homologous recombination mechanisms and non-homologous end-joining (NHEJ) mechanisms of recombination have been defined by using these approaches. Here I focus on recombination events that lead to alterations of chromosome structure: transpositions, translocations, deletions, DNA fragment capture and other small insertions. These rearrangements can occur from ectopic gene conversions accompanied by crossing-over, break-induced replication, single-strand annealing or non-homologous end-joining.
Haber
James E
JE
MS029 Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454-9110, USA. haber@brandeis.edu
D019457:Chromosome Breakage; D002872:Chromosome Deletion; D015825:Chromosomes, Fungal; D004260:DNA Repair; D004261:DNA Replication; D004251:DNA Transposable Elements; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D049770:Genes, Mating Type, Fungal / Q000235:genetics*; D008957:Models, Genetic; D051135:Rad51 Recombinase / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales / Q000235:genetics*; D014178:Translocation, Genetic
D004251:DNA Transposable Elements; D029701:Saccharomyces cerevisiae Proteins; D051135:Rad51 Recombinase; C530845:HO protein, S cerevisiae; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific