Survival and differentiation of embryonic neural explants on different biomaterials.
Biomaterials prepared from polyacrylamide, ethyl acrylate (EA), and hydroxyethyl acrylate (HEA) in various blend ratios, methyl acrylate and chitosan, were tested in vitro as culture substrates and compared for their ability to be colonized by the cells migrating from embryonic brain explants. Neural explants were isolated from proliferative areas of the medial ganglionic eminence and the cortical ventricular zone of embryonic rat brains and cultured in vitro on the different biomaterials. Chitosan, poly(methyl acrylate), and the 50% wt copolymer of EA and HEA were the most suitable substrates to promote cell attachment and differentiation of the neural cells among those tested. Immunofluorescence microscopy analysis showed that progenitor cells had undergone differentiation and that the resulting glial and neuronal cells expressed their intrinsic morphological characteristics in culture.
Soria
José Miguel
JM
Fundación Hospital General Universitario de Valencia, Avda Tres Cruces s/n, 46014 Valencia, Spain. soria_jos@gva.es
Martínez Ramos
Cristina
C
Salmerón Sánchez
Manuel
M
Benavent
Veronique
V
Campillo Fernández
Alberto
A
Gómez Ribelles
José Luis
JL
García Verdugo
José Manuel
JM
Pradas
Manuel Monleón
MM
Barcia
Juan Antonio
JA
D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D066298:In Vitro Techniques; D008422:Materials Testing; D009417:Nerve Tissue / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000166:cytology
D001672:Biocompatible Materials