title: Black beetle virus: propagation in Drosophila line 1 cells and an infection-resistant subline carrying endogenous black beetle virus-related particles.
abstract: Black beetle virus (BBV), one of a recently discovered class of viruses with a bipartite genome, multiplied readily in Schneider's line 1 of Drosophila cells. Virus yields, on the order of 100 mg per liter of culture, were unusually high and represented some 20% of the total cell protein within 3 days after infection. A derivative subline of these Drosophila cells was found to be resistant to infection by BBV. These resistant cells were also found to carry small amounts of BBV-related particles, possibly a maturation-defective form of BBV.
authors.affiliation: Biophysics Laboratory of the Graduate School and Biochemistry Department of the College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706.
authors.forename: P
authors.initials: P
authors.lastname: Friesen
authors.forename: P
authors.initials: P
authors.lastname: Scotti
authors.forename: J
authors.initials: J
authors.lastname: Longworth
authors.forename: R
authors.initials: R
authors.lastname: Rueckert