[Kinetic study of various cytokine mRNA expressions in rhesus treated with haploidentical peripheral blood stem cell transplantation].
This study was aimed to analyze the mRNA expression of cytokines (TGF-beta, IL-2, IL-6, IL-10, IFN-gamma, TNF-alpha, FAS-L) in five rhesus treated with haploidentical peripheral blood stem cell transplantation after nonmyeloablative preparative regimens and to explore the role of these cytokines in the development and pathology of acute graft-versus-host-disease (aGVHD). Five rhesus monkeys received nonmyeloablative haploidentical peripheral blood stem cells transplantation. Semi-quantitative reversed transcription polymerase chain reaction (RT-PCR) was used to analyze the kinetics of cytokine mRNA expression in the transplantation and aGVHD. The results showed that five rhesus monkeys acquired hematopoietic reconstitution successfully. The graft was rejected in one monkey which survived without disease, the other four achieved mixed chimerism and full donor chimerism. Chimerism of low centigrade in one monkey achieved high centigrade at 35 days after donor stem cell infusion. Intestinal aGVHD grade III developed in one monkey. Cytokines of Th1 and Th2 changed after transplantation. In period of aGVHD, expression of TGF-beta decreased but all others increased in various levels. When donor chimerism decreased, the cytokines decreased accordingly. It is concluded that the decrease of TGF-beta mRNA may be an indicator to predict aGVHD, and can be used as a differential diagnostic indicator for intestinal GVHD.
Huang
Ya-Jing
YJ
Department of Hematology, Hospital Affiliated to Academy Military Medical Sciences, Beijing 100071, China.
Sun
Qi-Yun
QY
Liu
Li-Hui
LH
Hu
Kai-Xun
KX
Fan
Chuan-Bo
CB
Bian
Li
L
Guo
Mei
M
Ai
Hui-Sheng
HS
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000378:metabolism*; D006238:Haploidy; D008253:Macaca mulatta; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
D016207:Cytokines; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta