MALDI analysis of oligonucleotides directly from montmorillonite.
Oligonucleotides synthesized on a montmorillonite catalyst were analyzed directly. By mixing the catalyst with a matrix (2,4,6-trihydroxyacetophenone or 6-aza-2-thiothymine) and dibasic ammonium citrate, higher molecular weight products were detected compared with "classical" methods such as gel electrophoresis and HPLC with UV as a detector. The oligomers (30-mers and higher) were detected by mass spectrometry even though their concentration was less than 10(-4)% of the total content of the RNA. This method is different from the (MALDI) analysis of the eluates from montmorillonite, which otherwise requires desalting. Placing reaction mixtures with a high concentration of buffers on homoionic, preferably Li-containing, montmorillonite does not require desalting.
Zagorevskii
Dmitri V
DV
Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute,209C Cogswell Laboratory, Troy, New York 12180, USA. zagord@rpi.edu
Aldersley
Michael F
MF
Ferris
James P
JP
D001546:Bentonite / Q000737:chemistry*; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009841:Oligonucleotides; D001546:Bentonite