title: Inheritance and mapping of a powdery mildew resistance gene introgressed from Avena macrostachya in cultivated oat.
abstract: The powdery mildew resistance from Avena macrostachya was successfully introgressed into hexaploid oat (A. sativa). Genetic analysis of F(1), F(2), F(3) and BC(1) populations from two powdery-mildew resistant introgression lines revealed that the resistance is controlled by a dominant gene, tentatively designated Eg-5. Molecular marker analysis was conducted using bulked-segregant analysis in two segregating F(3) populations. One codominant simple sequence repeats (SSR) marker AM102 and four AFLP-derived PCR-based markers were successfully developed. The SSR marker AM102 and the STS marker ASE41M56 were linked to the gene Eg-5, with genetic distances of 2 and 0.4 cM, respectively, in both mapping populations. Three STS markers (ASE45M56, ASE41M61, ASE36M55) co-segregated with Eg-5 in one population while two (ASE45M56, ASE36M55) of them linked to Eg-5 with a genetic distance of 1 cM in another population. The gene was further mapped to be in a region corresponding to linkage group 22_44+18 in the Kanota x Ogle (KO) hexaploid oat map by comparative mapping. To our knowledge, this is the first report of mapping powdery-mildew resistance in hexaploid oat. The new resistance source of A. macrostachya, together with the tightly linked markers identified here, could be beneficial in oat breeding programmes.
authors.affiliation: Institute of Agricultural Crops, Federal Centre for Breeding Research on Cultivated Plants (BAZ), Rudolf-Schick-Platz 3a, 18190, Gross Lüsewitz, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.forename: M
authors.initials: M
authors.lastname: Herrmann
mesh_terms: D001203:Ascomycota; D018554:Avena / Q000235:genetics* / Q000382:microbiology*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D007113:Immunity, Innate / Q000235:genetics; D040582:Inheritance Patterns; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D006109:Poaceae / Q000235:genetics* / Q000382:microbiology; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers