The C terminus of the immunophilin PASTICCINO1 is required for plant development and for interaction with a NAC-like transcription factor.
PASTICCINO1 (PAS1) is a high molecular weight FK506-binding protein (FKBP) involved in the control of cell proliferation and differentiation during plant development. Mutations in the C-terminal region of PAS1 result in severe developmental defects. We show here that the C-terminal domain of PAS1 controls the subcellular distribution of this protein. We also demonstrated in vitro and in vivo, by Forster resonance energy transfer, that this C-terminal region is required for interaction with FAN (FKBP-associated NAC), a new member of the plant-specific family of NAC transcription factors. PAS1 and FAN are translocated into the nucleus upon auxin treatment in plant seedlings. The nuclear translocation of PAS1 is dependent on the presence of the C terminus of the protein. Finally, we showed that FAN is involved in PAS1-regulated processes because FAN overproduction partly complemented the pas1 phenotype. We suggest that PAS1 regulates the function of this NAC-like transcription factor by controlling its targeting to the nucleus upon plant cell division.
Smyczynski
Cybelle
C
Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, INRA, Route de St. Cyr, 78026 Versailles Cedex, France.
Roudier
François
F
Gissot
Lionel
L
Vaillant
Emilie
E
Grandjean
Olivier
O
Morin
Halima
H
Masson
Thimoté
T
Bellec
Yannick
Y
Geelen
Danny
D
Faure
Jean-Denis
JD
D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry*; D002467:Cell Nucleus / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D005786:Gene Expression Regulation; D017343:Genes, Plant; D008969:Molecular Sequence Data; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry
D029681:Arabidopsis Proteins; C111981:PAS1 protein, Arabidopsis; D010940:Plant Proteins; D014157:Transcription Factors