[Full length nucleotide sequence analysis of L and M segments of Hantavirus Q32 strain gene].
BACKGROUND
To study the full length L and M sequence of Hantavirus Q32 strain gene and explore its molecular characters.


METHODS
The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.


RESULTS
The L genome segment of Q32 virus was found to be 6533 nucleotides in length. One large open reading frame was found located at bases 38 to 6493. This was predicted to encode an L protein 2151 amino acids in length with a molecular mass of 2.46 x 10(5). The M genome segment was 3616 nucleotides in length. One open reading frame was located at bases 41 to 3488. This was predicted to encode an M protein 1135 amino acids with a molecular mass of 1.26 x 10(5).


CONCLUSIONS
The nucleotides sequence of M and L segments of strain Q32 was similar to that of other Hantavirus M and L segments. Deduced amino acid sequences of glycoprotein and RNA polymerase revealed high homologue to other Hantavirus.
Dai
Xiao-xia
XX
Department of Immunology and Microbiology, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Medical School, Xi'an Jiaotong University, Xi'an 710061, China.
Zhang
Quan-fu
QF
Wang
Shi-wen
SW
Chu
Yong-lie
YL
Hao
Yong-hua
YH
Wang
Qin
Q
Xiu
Mei-hong
MH
Zhang
Ying
Y
Li
De-xin
DX
D000595:Amino Acid Sequence; D000818:Animals; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006476:Orthohantavirus / Q000235:genetics*; D008969:Molecular Sequence Data; D051189:Murinae; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014709:Vero Cells; D014763:Viral Matrix Proteins / Q000145:classification / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D018076:DNA, Complementary; D012367:RNA, Viral; D014763:Viral Matrix Proteins; D014764:Viral Proteins