Methods for studying adaptation to the DNA damage checkpoint in yeast.
When yeast are faced with irreparable DNA damage, they will first arrest in G2/M, via the DNA damage checkpoint pathway, but will subsequently adapt to that arrest and resume division. Here, we summarize assays that we have used to examine checkpoint adaptation. Specifically, we discuss the merits of inducing DNA damage with ionizing radiation (IR) and IR-mimetic drugs, HO, and the cdc13-1 mutation. We also discuss readouts that we have used to visualize adaptation.
Toczyski
David P
DP
Department of Biochemisty and Biophysics, Cancer Research Institute, University of California, San Francisco, USA.
D002453:Cell Cycle; D002872:Chromosome Deletion; D004249:DNA Damage; D007259:Infrared Rays; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D034501:Telomere-Binding Proteins / Q000235:genetics
C452219:Cdc13 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D034501:Telomere-Binding Proteins