title: [Knockdown of Hsp701A induces K562 cells apoptosis by RNA interference].
abstract: OBJECTIVE
  To investigate the apoptosis of K562 cells induced by RNA interference(RNAi) targeting Hsp701A.
  
  
  METHODS
  Small interference RNA (siRNA) targeting Hsp701A was generated and its eukaryotic expression vectors was constructed and transected into K562 cells. Proliferation, apoptosis and cell cycle of the transected cells was respectively detected by MTT colorimetry and FCM.
  
  
  RESULTS
  The eukaryotic expression vector of siRNA against Hsp701A was successfully constructed and transfected into K562 cells, which markedly decreased the expression of Hsp701A on both mRNA and protein level. K562 cells transfected with the vector exhibited slower proliferation, increased apoptosis and increased G(0)/G(1) arrest.
  
  
  CONCLUSIONS
  The inhibition of Hsp701A gene by RNAi may provide a foundation for the development of novel therapeutic strategy for chronic granulocyte leukemia (CML).
authors.affiliation: Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. araa@sohu.com
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Wang-gang
authors.initials: WG
authors.lastname: Zhang
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: An-gang
authors.initials: AG
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D004262:DNA Restriction Enzymes / Q000378:metabolism; D055785:Gene Knockdown Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000628:therapy; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; C081884:heat-shock protein 70.1; D004262:DNA Restriction Enzymes