title: Potentiation of the bactericidal activity of Harungana madagascariensis Lam. ex Poir. (Hypericaceae) leaf extract against oral bacteria using poly (D, L-lactide-co-glycolide) nanoparticles: in vitro study.
abstract: Harungana madagascariensis Lam. ex Poir. (Hypericaceae) is known to have biological properties with mainly antibacterial, antifungal, and antiviral effects. The objective of this study was to investigate the in vitro bactericidal activity of the ethyl acetate H. madagascariensis leaf extract (HLE) on the main oral bacterial strains largely implicated in dental caries and gingivitis infections, and the possibility of potentialization of HLE antibacterial effects using the poly (D,L-lactide-co-glycolide) nanoparticles (PLG-NP). The microdilution technique and the interfacial polymer deposition following the solvent diffusion method were used to investigate the in vitro bactericidal activity of ethyl acetate HLE and to prepare nanoparticles, respectively. HLE showed significant bactericidal effects against the bacterial strains tested, with minimal bactericidal concentration (MBC) to 5 x 10(2) mg/l or less, except for Lactobacillus casei with 7.5 x 10(2) mg/l. With the HLE incorporated into PLG nanoparticles (HLE-PLG-NP), we observed diminution of the bactericidal concentration compared to HLE, the upper MBC being of 1.875 x 10(2) mg/l. Incorporation of the HLE into a colloidal carrier optimized its antibacterial performance.
authors.affiliation: Equipe Sciences Séparatives et Biopharmaceutiques UFR Sciences Médicales et Pharmaceutiques, Université de Franche-Comté, EA-3924, Place Saint Jacques, Besançon, France. bricemoulari@msn.com
authors.forename: Brice
authors.initials: B
authors.lastname: Moulari
authors.forename: Hassan
authors.initials: H
authors.lastname: Lboutounne
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Chaumont
authors.forename: Yves
authors.initials: Y
authors.lastname: Guillaume
authors.forename: Joëlle
authors.initials: J
authors.lastname: Millet
authors.forename: Yann
authors.initials: Y
authors.lastname: Pellequer
mesh_terms: D000085:Acetates; D000190:Actinomyces / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology*; D028084:Clusiaceae; D004337:Drug Carriers; D004357:Drug Synergism; D005673:Fusobacterium / Q000187:drug effects; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology*; D007778:Lactobacillus / Q000187:drug effects; D049329:Nanostructures; D010936:Plant Extracts / Q000494:pharmacology*; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000494:pharmacology*; D018720:Prevotella / Q000187:drug effects; D011424:Propionibacterium / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects
chemical_list: D000085:Acetates; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D010936:Plant Extracts; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C007650:ethyl acetate