Protective immunity in rats by intranasal immunization with Streptococcus mutans glucan-binding protein D encapsulated into chitosan-coated poly(lactic-co-glycolic acid) microspheres.
Recombinant Streptococcus mutans glucan-binding protein D (rGbpD) was incorporated into poly(lactic-co-glycolic acid) (PLGA) microspheres which then were surface-coated with chitosan. The microspheres, with a mean diameter of ca. 1.8 microm, were intranasally administered in rats. There were elevated salivary immunoglobulin A and serum immunoglobulin G antibody responses to rGbpD, as well as lower molar caries scores in immunized animals as compared to sham immunized ones. The chitosan-coated PLGA microspheres are thus potentially useful for antigen delivery in dental caries vaccination.
Zhao
Hongping
H
Department of Operative Dentistry and Endodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, P.R. China.
Wu
Buling
B
Wu
Hong
H
Su
Lingyun
L
Pang
Jianliang
J
Yang
Tiehong
T
Liu
Yanli
Y
D000281:Administration, Intranasal; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D008297:Male; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012463:Saliva / Q000378:metabolism; D013295:Streptococcus mutans / Q000276:immunology* / Q000378:metabolism*; D013499:Surface Properties
D002352:Carrier Proteins; D037102:Lectins; D011108:Polymers; C037937:glucan-binding proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan