[Knockdown of Hsp701A induces K562 cells apoptosis by RNA interference].
OBJECTIVE
To investigate the apoptosis of K562 cells induced by RNA interference(RNAi) targeting Hsp701A.


METHODS
Small interference RNA (siRNA) targeting Hsp701A was generated and its eukaryotic expression vectors was constructed and transected into K562 cells. Proliferation, apoptosis and cell cycle of the transected cells was respectively detected by MTT colorimetry and FCM.


RESULTS
The eukaryotic expression vector of siRNA against Hsp701A was successfully constructed and transfected into K562 cells, which markedly decreased the expression of Hsp701A on both mRNA and protein level. K562 cells transfected with the vector exhibited slower proliferation, increased apoptosis and increased G(0)/G(1) arrest.


CONCLUSIONS
The inhibition of Hsp701A gene by RNAi may provide a foundation for the development of novel therapeutic strategy for chronic granulocyte leukemia (CML).
Zhu
Qing
Q
Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. araa@sohu.com
Zhang
Wang-gang
WG
Wang
Li-feng
LF
Wang
Fang
F
Zhang
Yong
Y
Yang
An-gang
AG
D017209:Apoptosis / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D004262:DNA Restriction Enzymes / Q000378:metabolism; D055785:Gene Knockdown Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000628:therapy; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; C081884:heat-shock protein 70.1; D004262:DNA Restriction Enzymes