title: Chronic restraint stress modulates expression of genes in murine spleen.
abstract: Psychological and physical stress can alter the immune system in both humans and animals. We have reported that mice subjected to chronic 12-h daily physical restraint for 2 days showed dramatic apoptosis in splenocytes. To identify genes that contribute to the splenocyte apoptosis, we compare gene expression in the spleens of restrained and unstressed mice using oligo microarrays consisting of 226 genes. We report here that mice subjected to chronic 12-h daily physical restraint for 2 days exhibited significantly altered expression of 50 of 226 genes. These genes included pro-apoptotic genes. We selected 5 genes of interest and confirmed the microarray results by real-time PCR. In this study, we identify a potentially important component of pro-apoptotic activity in restraint stress and suggest a possible target for anti-apoptotic therapy to protect splenocytes against stress-induced apoptosis.
authors.affiliation: Department of Internal Medicine, James Quillen College of Medicine, East Tennessee State University, P. O. Box 70622, Johnson City, TN 37614, United States. yin@etsu.edu
authors.forename: Deling
authors.initials: D
authors.lastname: Yin
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Charles
authors.initials: C
authors.lastname: Stuart
authors.forename: Junying
authors.initials: J
authors.lastname: Miao
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Zeng
authors.forename: Gregory
authors.initials: G
authors.lastname: Hanley
authors.forename: Jonathan
authors.initials: J
authors.lastname: Moorman
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yao
authors.forename: Michael
authors.initials: M
authors.lastname: Woodruff
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002908:Chronic Disease; D004195:Disease Models, Animal; D053200:Fas-Associated Death Domain Protein; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics* / Q000276:immunology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D024101:Mitochondrial Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012149:Restraint, Physical; D015398:Signal Transduction / Q000235:genetics; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D013315:Stress, Psychological / Q000235:genetics* / Q000276:immunology* / Q000503:physiopathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology; D019014:fas Receptor / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C508686:BNip3 protein, mouse; C505534:Fadd protein, mouse; D053200:Fas-Associated Death Domain Protein; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor