Ethanol vapor self-administration in adult C57BL/6J male mice.
To determine if ethanol vapor is reinforcing, chambers with two nose-poke devices were used. First, C57BL/6J mice were trained to nose-poke at one (FR port) and then to hold a nose-poke for at least 1 s at the second (reward port) for 10% sucrose reinforcement. When mice held reward port nose-pokes for > or = 1 s on 95% of session trials, ethanol vapor (0.05%, w/v) or clean air replaced sucrose. In experiment 1, the FR requirement at the FR port was incremented from 1 to 8. Ethanol vapor (n=3) or clean air (n=3) was delivered for 1 s following a single nose-poke at the reward port after completing response requirements at the FR port. Nose-pokes at the FR port increased with increasing FR to maintain ethanol vapor intake, while clean air intake and its associated responding extinguished. In experiment 2, mice (n=4) controlled the duration of ethanol vapor delivery by providing vapor for as long as the mouse held a single poke in the reward port after completing response requirements at the FR port. Nose-pokes at the FR port increased with increasing FR to maintain ethanol vapor intake at the reward port. These findings indicate that ethanol vapor is reinforcing in adult C57BL/6J male mice.
Kantak
Kathleen M
KM
Laboratory of Behavioral Neuroscience, Department of Psychology and Program in Neuroscience, Boston University, Boston, MA 02215, United States. kkantak@bu.edu
Luzzo
Christopher
C
D000280:Administration, Inhalation; D000818:Animals; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage* / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012055:Reinforcement Schedule; D013997:Time Factors
D000431:Ethanol