Isolation of infectious xenotropic mouse type C virus by transfection of a heterologous cell with DNA from a transformed mouse cell.
An endogenous xenotropic type C virus has been isolated from a Kirsten sarcoma virus-transformed BALB/c mouse cell line by transfection of a mink fibroblast cell with the DNA from the transformed cells. The results indicate that transfection may be used as a technique to isolate this endogenous type C virus without the need to chemically induce the cell line containing the provirus prior to attempting to isolate the virus.
Scolnick
E M
EM
Viral Leukemia and Lymphoma Branch, National Cancer Institute, Bethesda, Maryland 20014.
Bumgarner
S J
SJ