title: ZNF569, a novel KRAB-containing zinc finger protein, suppresses MAPK signaling pathway.
abstract: Transcription factors play an essential role in altering gene expression. A great progress about transcription factors has been made towards the understanding of normal physiological processes, embryonic development, and human diseases. Here we report the identification and characterization of a novel KRAB-containing zinc-finger protein, ZNF569, which is isolated from a human embryonic heart cDNA library. ZNF569 encodes a putative protein of 686 amino acids. The protein is conserved across different species during evolution. Expression of ZNF569 was found in most of the examined human adult and embryonic tissues with a higher level in heart and skeletal muscles. The KRAB and ZNF motifs of ZNF569 represent potent repression domains. When ZNF569 is fused to Gal-4 DNA-binding domain and co-transfected with VP-16, ZNF569 protein suppresses transcriptional activity. Overexpression of ZNF569 in COS-7 cells inhibited the transcriptional activities of SRE and AP-1, which may be silenced by siRNA. The results suggest that ZNF569 protein may act as a transcriptional repressor that suppresses MAPK signaling pathway to mediate cellular functions.
authors.affiliation: The Center for Heart Development, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, People's Republic of China.
authors.forename: Xinqiong
authors.initials: X
authors.lastname: Huang
authors.forename: Wuzhou
authors.initials: W
authors.lastname: Yuan
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Bai
authors.forename: Yun
authors.initials: Y
authors.lastname: Deng
authors.forename: Chuanbing
authors.initials: C
authors.lastname: Zhu
authors.forename: Pei
authors.initials: P
authors.lastname: Liang
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Du
authors.forename: Mingyao
authors.initials: M
authors.lastname: Liu
authors.forename: Yuequn
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiushan
authors.initials: X
authors.lastname: Wu
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D016153:Genes, Suppressor; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D020935:MAP Kinase Signaling System; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D018808:Transcription Factor AP-1; C512510:ZNF569 protein, human