title: Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes: an interlaboratory study.
abstract: The objective of this study was to investigate whether 24 h exposure to radiofrequency electromagnetic fields similar to those emitted by mobile phones induces genotoxic effects and/or effects on cell cycle kinetics in cultured human peripheral blood lymphocytes. The effect of 900 MHz exposure (GSM signal) was evaluated at four specific absorption rates (SARs, 0, 1, 5 and 10 W/kg peak values). The exposures were carried out in wire patch cells under strictly controlled conditions of both temperature and dosimetry, and the induction of genotoxic effects was evaluated in lymphocyte cultures from 10 healthy donors by applying the cytokinesis-block micronucleus assay. Positive controls were provided by using mitomycin C. Two research groups were involved in the study, one at ENEA, Rome, and the other at CNR-IREA, Naples. Each laboratory tested five donors, and the resulting slides were scored by both laboratories. Following this experimental scheme, it was also possible to compare the results obtained by cross-scoring of slides. The results obtained provided no evidence for the existence of genotoxic or cytotoxic effects in the range of SARs investigated. These findings were confirmed in the two groups of five donors examined in the two laboratories and when the same slides were scored by two operators.
authors.affiliation: Interuniversity Center for Interaction between Electromagnetic Fields and Biosystems (ICEmB) at CNR-Institute for Electromagnetic Sensing of Environment (IREA), 80124 Naples, Italy. scarfi.mr@irea.cnr.it
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Scarfì
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Fresegna
authors.forename: Paola
authors.initials: P
authors.lastname: Villani
authors.forename: Rosanna
authors.initials: R
authors.lastname: Pinto
authors.forename: Carmela
authors.initials: C
authors.lastname: Marino
authors.forename: Maurizio
authors.initials: M
authors.lastname: Sarti
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Altavista
authors.forename: Anna
authors.initials: A
authors.lastname: Sannino
authors.forename: Giorgio A
authors.initials: GA
authors.lastname: Lovisolo
mesh_terms: D000328:Adult; D040421:Cell Phone; D049109:Cell Proliferation / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004781:Environmental Exposure; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects* / Q000706:statistics & numerical data; D015162:Micronucleus Tests; D008872:Microwaves; D011829:Radiation Dosage; D011846:Radio Waves