title: On-chip chiral separation based on bovine serum albumin-conjugated carbon nanotubes as stationary phase in a microchannel.
abstract: A novel method of chiral separation based on protein-stationary phase immobilized in a poly(methyl methacrylate) microfluidic chip was developed. BSA conjugated with the shortened carboxylic single-walled carbon nanotubes (SWNTs) was employed as the chiral selector. Successful separation of tryptophan enantiomers was achieved in less than 70 s with a resolution factor of 1.35 utilizing a separation length of 32 mm. This is the first example of chiral separation based on SWNTs-BSA conjugates as stationary phase immobilized in microchip channel. The stability of the stationary phase in the channel was examined by microchip electrophoresis with laser-induced fluorescence detection. Factors that influenced the chiral separation resolution were examined. Under the optimized conditions, the proposed modified chip revealed adequate repeatability concerning run-to-run. These results show that the use of SWNTs-BSA conjugates within microfluidic channels hold great promise for a variety of analytical schemes.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, P. R. China.
authors.forename: Xuexiang
authors.initials: X
authors.lastname: Weng
authors.forename: Hongyan
authors.initials: H
authors.lastname: Bi
authors.forename: Baohong
authors.initials: B
authors.lastname: Liu
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
mesh_terms: D002244:Carbon / Q000737:chemistry*; D004560:Electricity; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013237:Stereoisomerism; D014364:Tryptophan / Q000737:chemistry
chemical_list: D012710:Serum Albumin, Bovine; D002244:Carbon; D014364:Tryptophan