title: The structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O36 containing 3-deoxy-d-manno-oct-2-ulosonic acid.
abstract: An oligosaccharide that corresponds to the repeating unit of the O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O36. Structural studies of the oligosaccharide and O-deacylated lipopolysaccharide were performed using sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, and H-detected (1)H,(13)C HSQC and HMBC experiments. It was found that the O-polysaccharide is built up of linear trisaccharide repeating units containing 2-acetamido-2-deoxyglucose, 6-deoxy-l-talose (l-6dTal), and 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) and has the following structure. [structure: see text]
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Nina A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: Olga G
authors.initials: OG
authors.lastname: Ovchinnikova
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Torzewska
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Antoni
authors.initials: A
authors.lastname: Rozalski
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis; D002240:Carbohydrate Sequence; D003837:Deoxy Sugars / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D011532:Providencia / Q000737:chemistry*; D013400:Sugar Acids / Q000032:analysis*
chemical_list: D003837:Deoxy Sugars; D006601:Hexoses; D019081:O Antigens; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; C011754:6-deoxytalose; D000117:Acetylglucosamine