Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection.
We developed a microfluidic chip-affinity CE method based on indirect LIF detection to study protein-drug interactions. The interaction between heparin and BSA was quantitatively studied, as a model system. In our method, sodium fluorescein was chosen as background, and redistilled water as marker to monitor EOF. The electrophoretic mobility changes of BSA were measured, with various concentrations of heparin added to the running buffer. Each run was completed within 80 s. The binding constant was determined to be (1.24 +/- 0.05) x 10(3) M(-1), which was in good agreement with that reported in the literature.
Liu
Xiaojun
X
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, P. R. China.
Liu
Xin
X
Liang
Aiye
A
Shen
Zheng
Z
Zhang
Yu
Y
Dai
Zhongpeng
Z
Xiong
Bohui
B
Lin
Bingcheng
B
D046209:Electrophoresis, Microchip / Q000379:methods*; D007834:Lasers; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004364:Pharmaceutical Preparations; D012710:Serum Albumin, Bovine