Protections of pinocembrin on brain mitochondria contribute to cognitive improvement in chronic cerebral hypoperfused rats.
To study effects of pinocembrin, a natural compound extracted from propolis, on cognitive ability impaired by chronic cerebral hypoperfusion in rats, and if it did so, to investigate its effects on brain mitochondria. Rat chronic cerebral hypoperfusion was achieved by permanent bilateral common carotid arteries ligation, with regional cerebral blood flow evaluated. Cognitive ability was tested by Morris water maze task. Production of reactive oxygen species and origin targets including mitochondria membrane potential, respiratory chain complex I, complex III activities and mitochondria swelling degree were evaluated. Cytochrome oxidase was determined on its expression level by western blotting. Pinocembrin alleviated cognitive impairments in Morris water maze and decreased mitochondria reactive oxygen species production, in accordance with its improvements on complex I activity, membrane potential level, mitochondria swelling degree and cytochrome oxidase deficits. Pinocembrin could improve rat cognitive impairments induced by chronic cerebral hypoperfusion, contributed to its protections on brain mitochondria structure and function.
Guang
Hong-Mei
HM
National Center for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xian Nong Tan Street, Beijing 100050, PR, China.
Du
Guan-Hua
GH
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D017536:Carotid Artery, Common / Q000601:surgery; D002560:Cerebrovascular Circulation / Q000187:drug effects; D003072:Cognition Disorders / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D042967:Electron Transport Complex I / Q000378:metabolism; D014450:Electron Transport Complex III / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology*; D015151:Immunoblotting; D017078:Laser-Doppler Flowmetry; D008026:Ligation / Q000009:adverse effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000502:physiology; D008933:Mitochondrial Swelling / Q000187:drug effects; D011429:Propolis / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D044950:Flavanones; C016063:pinocembrin; D011429:Propolis; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III