title: Antimutagenic and antioxidant properties of phenolic fractions from Andean purple corn (Zea mays L.).
abstract: The antimutagenic and antioxidant properties of various phenolic fractions obtained from Andean purple corn were examined by the Ames test and the DPPH antiradical assay. An anthocyanin-rich water fraction (WF) and an ethyl acetate fraction (EAF) showed a dose-dependent antimutagenic behavior against the food mutagen Trp-P-1 with IC50 values of 321.7 +/- 21.36 and 95.2 +/- 10.95 microg of chlorogenic acid equiv/plate, respectively, indicating that EAF was a more potent antimutagen. The antioxidant activities for WF and EAF were 1.019 +/- 0.05 and 0.838 +/- 0.11 microg of Trolox equiv/mug of phenolics, respectively. Further fractionation of WF and EAF revealed an ethyl acetate subfraction, EA-IV, with high antimutagen potency that contained a quercetin derivative. The mechanism of antimutagenic action of the WF is predominantly a blocking effect on the S-9 Mix activation system of the mutagen, whereas for the EAF, it is a dual mechanism involving blocking of the S-9 Mix and a scavenging action on Trp-P-1 electrophiles.
authors.affiliation: Department of Horticultural Sciences, Texas A&M University, College Station, Texas 77843-2133, USA.
authors.forename: Romina
authors.initials: R
authors.lastname: Pedreschi
authors.forename: Luis
authors.initials: L
authors.lastname: Cisneros-Zevallos
mesh_terms: D000085:Acetates; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D005609:Free Radicals / Q000037:antagonists & inhibitors; D009152:Mutagenicity Tests; D010636:Phenols / Q000494:pharmacology*; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology; D014867:Water; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000085:Acetates; D016587:Antimutagenic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D014867:Water; C007650:ethyl acetate; C004931:1,1-diphenyl-2-picrylhydrazyl