title: Enhancement and selective production of oligomycin through inactivation of avermectin's starter unit in Streptomyces avermitilis.
abstract: Oligomycin and its analogues, produced by Streptomyces avermitilis and other actinomycetes, are of interest for their potent and selective biological activities. PCR-mediated gene replacement, targeting bkdF, one of avermectin's starter unit encoding genes in S. avermitilis, was performed to yield an oligomycin producer, BIB0423. The engineered strain produced oligomycin A at 2.3 mg/ml compared to the wild type strain at 0.1 mg/ml. This resulting mutant was genetically stable and should be useful for the industrial production of oligomycin.
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Wei
authors.forename: Liang
authors.initials: L
authors.lastname: Yunxiang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yinghua
mesh_terms: D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000378:metabolism; D009840:Oligomycins / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D009840:Oligomycins; D007559:Ivermectin; C019264:avermectin