Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay.
Newcastle disease virus (NDV) strains can be classified as virulent or avirulent based upon the severity of the disease. Differentiation of the virus into virulent and avirulent is necessary for effective control of the disease. Biopanning experiments were performed using a disulfide constrained phage displayed heptapeptide library against three pathotypes of NDV strains: velogenic (highly virulent), mesogenic (moderately virulent) and lentogenic (avirulent). A phage clone bearing the peptide sequence SWGEYDM capable of distinguishing virulent from avirulent NDV strains was isolated. This phage clone was employed as a diagnostic reagent in a dot blot assay and it successfully detected only virulent NDV strains.
Lee
Thong Chuan
TC
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Yusoff
Khatijah
K
Nathan
Sheila
S
Tan
Wen Siang
WS
D000818:Animals; D001717:Birds / Q000821:virology; D015151:Immunoblotting / Q000379:methods*; D009522:Newcastle disease virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification; D011485:Protein Binding; D012680:Sensitivity and Specificity; D014774:Virulence
D019151:Peptide Library; D010455:Peptides