A simple parallel analytical method of prenatal screening.
Protein microarray has progressed rapidly in the past few years, but it is still hard to popularize it in many developing countries or small hospitals owing to the technical expertise required in practice. We developed a cheap and easy-to-use protein microarray based on dot immunogold filtration assay for parallel analysis of ToRCH-related antibodies including Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus type 1 and 2 in sera of pregnant women. It does not require any expensive instruments and the assay results can be clearly recognized by the naked eye. We analyzed 186 random sera of outpatients at the gynecological department with our microarray and commercial ELISA kit, and the results showed there was no significant difference between the two detection methods. Validated by clinical application, the microarray is easy to use and has a unique advantage in cost and time. It is more suitable for mass prenatal screening or epidemiological screening than the ELISA format.
Li
Ding
D
Institute of Genetic Diagnosis, School of Pharmacy, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an, China.
Yang
Hao
H
Zhang
Wen-Hong
WH
Pan
Hao
H
Wen
Dong-Qing
DQ
Han
Feng-Chan
FC
Guo
Hui-Fang
HF
Wang
Xiao-Ming
XM
Yan
Xiao-Jun
XJ
D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D016014:Linear Models; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D040081:Protein Array Analysis / Q000191:economics / Q000379:methods; D015203:Reproducibility of Results
D007074:Immunoglobulin G