Regulation of two photosynthetic pigment-related genes during stress-induced pigment formation in the green alga, Dunaliella salina.
The expression of mRNAs coding for 1-deoxyxylulose-5-phosphate synthase (DXS) and phytoene synthase (PSY) were studied in Dunaliella salina grown under nitrogen-sufficient (NS) and nitrogen-limited (NL) conditions. Under NS conditions growth was 2.5 times higher than under NL conditions. No differences were found in chlorophyll a content per cell, and total carotenoid content per cell was 5.33 pg 1(-1) for the NS treatment and 7.76 pg 1(-1) for the NL. DXS transcripts exhibited diminished expression under NL conditions, peaking at day 15 of cultivation in both treatments. Simultaneously, PSY transcripts exhibited constant expression under both conditions. These results suggest that these genes play an important role in the balance of photosynthetic pigments during pigment accumulation.
Sánchez-Estudillo
Leticia
L
Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN) Unidad Mérida, Antigua Carretera a Progreso Km. 6, Apdo. Postal 73, Cordemex, 97310 Mérida, Yucatan, Mexico.
Freile-Pelegrin
Yolanda
Y
Rivera-Madrid
Renata
R
Robledo
Daniel
D
Narváez-Zapata
José Alberto
JA
D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism*; D002338:Carotenoids / Q000235:genetics / Q000378:metabolism*; D000460:Chlorophyta / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D009584:Nitrogen / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D014166:Transferases / Q000235:genetics / Q000378:metabolism*
D045322:Photosynthetic Reaction Center Complex Proteins; D002338:Carotenoids; D014166:Transferases; C109461:deoxyxylulose-5-phosphate synthase; D019883:Alkyl and Aryl Transferases; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D009584:Nitrogen