Stabilization of a plasmid coding for a heterologous antigen in Salmonella enterica serotype typhi vaccine strain CVD908-htrA by using site-specific recombination.
A gene cassette incorporating the crs-rsd site-specific recombination system from the Salmonella enterica subsp. enterica serovar Dublin virulence plasmid improved the inheritance in S. enterica serotype Typhi strain CVD908-htrA of a multicopy plasmid expression vector. Use of this recombination cassette may improve expression of heterologous antigens from multicopy plasmid expression vectors in attenuated bacterial vaccine strains.
Stephens
Jonathan C
JC
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom.
Darsley
Michael J
MJ
Turner
Arthur K
AK
D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D015478:Antigens, Heterophile / Q000235:genetics* / Q000276:immunology; D005822:Genetic Vectors / Q000378:metabolism; D016480:Helicobacter pylori / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000378:metabolism*; D011995:Recombination, Genetic; D022562:Salmonella Vaccines / Q000235:genetics* / Q000276:immunology; D012485:Salmonella typhi / Q000235:genetics* / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology
D000942:Antigens, Bacterial; D015478:Antigens, Heterophile; D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated