title: [Transmission of ribosomal chromatin during mitosis in embryonal pig kidney cells (PK cell line)].
abstract: Mammalian nucleolar organizers (NORs) contain ribosomal (rRNA) genes associated with argyrophilic proteins and can be specifically stained by silver. These genes are clustered in four loci of PK meta- and submetacentric chromosomes 8 and 10. According to our data reported elsewhere, one of PK NORs contains a large amplification of rRNA that is manifested on silver stained chromosomes as abnormally large Ag+-NOR 8a. It is proposed that such a redundancy of rRNA genes induced their dose compensation in the form of transcriptional silencing, triggering the rise of Ag-negative ribosomal chromatin. To test this assumption, we carried out a morphometrical analysis of Ag+-NOR (bearing) chromosomes from prometaphase sets with modal chromosome number. We found that in the individual chromosome set a longer homologue 10L of chromosome 10 had always a larger Ag(+)-NOR area than did a shorter homologue 10S. Thus, PK karyotype consists of two pairs of heteromorphous Ag(+)-NORs: 8a and 8, 10L and 10S. One half of tested chromosome sets revealed four Ag(+)-NOR chromosomes, the other half had one Ag-negative NOR nearly equally belonging to chromosome either 8 or 10S. The majority of prometaphase Ag+-NORs showed partial or full chromatid splitting that allows for Ag(+)-NOR area measurements of (sister) chromatid. The area ratio of larger to smaller chromatid Ag(+)-NOR strongly varied for all chromosomes except chromosomes 8a. The maximum value of this ratio reached 5.1 for chromosome 8 and 3.4, and 2.3--for 10L and 10S (vs. 1.6 for 8a). Proportions of chromosomes with the ratio 1.25 and more, were: near two thirds--among chromosomes 8, near half--among 10L and 10S, but less than a quarter--among chromosomes 8a. These findings show that progressive differentiation of sister chromatid NORs in regard of the content of Ag-negative ribosomal chromatin may lead to an unequal Ag(+)-NOR distribution between daughter cells. To test this prediction, we developed a new technique for obtaining two dimensional (2D) preparations of stretched PK cells, which makes it possible to avoid the stage of hypotonic treatment of living cells, since this treatment levels the silver staining of NORs and prenucleolar bodies of fixed telophase cells. We found that some daughter nuclei from early telophase cells revealed the value of Ag(+)-NOR separation equal to 4:3 instead of the common value equal to 4:4. Complimentary 2D FISH with 28S + 18S mink rDNA probe showed that early telophase rRNA loci, detected as four bright large spots, are close by area and shape to Ag(+)-NORs of the corresponding cells. Sometimes, however, one of the daughter nuclei showed three such domains, in addition to one slight linear FISH signal that most probably represented Ag-negative NOR. A delayed separation of sister chromatids is the main structural characteristic of inactive chromatin (Azuara et al., 2003). It was established that the largest PK Ag(+)-NOR (chromosome 8a) showed a high level of cohesion and (or) twisting of sister chromatids that is characteristic of prophase rather than prometaphase PK chromosomes. These findings, together with the above cited literature data, give evidence for enrichment of Ag(+)-NOR by either inactive or (and) low active late-replicating chromatin.
authors.forename: V N
authors.initials: VN
authors.lastname: Stefanova
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Demin
mesh_terms: D000818:Animals; D006570:Heterochromatin / Q000235:genetics* / Q000648:ultrastructure; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D018374:LLC-PK1 Cells; D008938:Mitosis; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D034622:RNA Interference; D012335:RNA, Ribosomal / Q000235:genetics* / Q000648:ultrastructure; D013552:Swine
chemical_list: D006570:Heterochromatin; D012335:RNA, Ribosomal