title: Crystallization and preliminary X-ray studies of the DNA-binding domain of hepatocyte nuclear factor-6alpha complexed with DNA.
abstract: Hepatocyte nuclear factor 6 (HNF-6)/OC-1, a part of liver-enriched transcription factor, controls pancreas and liver development and regulates expression of several hepatic genes. DNA-binding region of HNF-6alpha bound to a 14-mer DNA fragment has been crystallized by the hanging drop vapor diffusion method. The crystals belong to space group P2 with unit cell parameters of a = 73.0 A, b = 39.0 A, c = 106.5 A, beta = 107.6 degrees. X-ray diffraction data were collected to 2.0 A resolution.
authors.affiliation: Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Iyaguchi
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Watanabe
authors.forename: Jun
authors.initials: J
authors.lastname: Nishihira
authors.forename: Isao
authors.initials: I
authors.lastname: Tanaka
mesh_terms: D001483:Base Sequence; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D051559:Hepatocyte Nuclear Factor 6 / Q000737:chemistry* / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data
chemical_list: D051559:Hepatocyte Nuclear Factor 6; D046911:Macromolecular Substances; D004247:DNA