title: Differentiation and regeneration of adrenal tissues: An initial step toward regeneration therapy for steroid insufficiency.
abstract: In animal experiments, adrenal cortical tissue has been successfully regenerated through xenotransplantation of cloned adrenocortical cells, suggesting that the intraadrenal stem cells required for such tissue formation may be present in the adrenal cortex. Stable expression of Ad4BP/SF-1, a key factor for adrenal and gonadal development and steroidogenesis, has been shown to direct embryonic stem cells toward the steroidogenic lineage. However, this steroidogenic capacity was very limited since progesterone was only produced in the presence of an exogenous substrate. Bone marrow mesenchymal cells are thought to contain pluripotent progenitor cells, which differentiate into multiple lineages. We have demonstrated that adenovirus-mediated forced expression of SF-1 in long-term cultured bone marrow cells can produce steroidogenic cells with the capacity for de novo synthesis of various steroid hormones in response to ACTH. This discovery may represent the first step in autologous cell transplantation therapy for patients with steroid hormone deficiency.
authors.affiliation: Department of Medicine and Bioregulatory Science (Third Department of Internal Medicine), Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Yanase
authors.forename: Shigeki
authors.initials: S
authors.lastname: Gondo
authors.forename: Taijiro
authors.initials: T
authors.lastname: Okabe
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tanaka
authors.forename: Hisao
authors.initials: H
authors.lastname: Shirohzu
authors.forename: Wuqiang
authors.initials: W
authors.lastname: Fan
authors.forename: Koichi
authors.initials: K
authors.lastname: Oba
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Morinaga
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nomura
authors.forename: Kenji
authors.initials: K
authors.lastname: Ohe
authors.forename: Hajime
authors.initials: H
authors.lastname: Nawata
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology / Q000502:physiology*; D000309:Adrenal Insufficiency / Q000628:therapy*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D017690:Cell Transplantation; D005260:Female; D015316:Genetic Therapy / Q000379:methods; D018398:Homeodomain Proteins / Q000502:physiology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology; D012038:Regeneration / Q000502:physiology*; D054339:Steroidogenic Factor 1; D013256:Steroids / Q000096:biosynthesis; D014157:Transcription Factors / Q000502:physiology
chemical_list: D018398:Homeodomain Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D013256:Steroids; D014157:Transcription Factors