psbA mutation (Asn266 to Thr) in Senecio vulgaris L. confers resistance to several PS II-inhibiting herbicides.
DNA sequence analysis of the psbA gene encoding the D1 protein of photosystem II (PS II), the target site of PS II-inhibiting herbicides, identified a point mutation (Asn266 to Thr) in a bromoxynil-resistant Senecio vulgaris L. population collected from peppermint fields in Oregon. Although this mutation has been previously reported in Synechocystis, this is the first report of this particular point mutation in a higher plant exhibiting resistance to PS II-inhibiting herbicides. The resistant population displayed high-level resistance to bromoxynil and terbacil (R/S ratio 10.1 and 9.3, respectively) and low-level resistance to metribuzin and hexazinone (R/S ratio 4.2 and 2.6, respectively) when compared with the susceptible population. However, the population was not resistant to the triazine herbicides atrazine and simazine or to the urea herbicide diuron. A chlorophyll fluorescence assay confirmed the resistance levels and patterns of cross-resistance of the whole-plant studies. The resistant S. vulgaris plants produced fewer seeds. Differences in cross-resistance patterns to PS II-inhibiting herbicides and the difference in fitness cost could be exploited in a weed management program.
Park
Kee Woong
KW
Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331-3002, USA. kee-woong.park@oregonstate.edu
Mallory-Smith
Carol A
CA
D004252:DNA Mutational Analysis; D004351:Drug Resistance / Q000235:genetics; D006540:Herbicides; D045332:Photosystem II Protein Complex / Q000037:antagonists & inhibitors / Q000235:genetics*; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D017354:Point Mutation; D021122:Protein Subunits / Q000235:genetics; D012674:Senecio / Q000187:drug effects* / Q000235:genetics
D006540:Herbicides; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D021122:Protein Subunits