[Gene expression analysis by DNA microarray in papillary thyroid cancer].
In our study we present chosen elements of microarray analysis of gene expression profile in papillary thyroid cancer. The study group included 16 papillary thyroid cancer tissues and 16 corresponding normal tissues. Samples were analyzed on high density oligonucleotide microarrays (GeneChip HG-U133A) which contain 22.000 genes. 110 genes, which had significant changed expression, were selected by MAS 5.0 program. 3 genes were chosen to the deeper analysis: dipeptidylpeptidase 4 (DPP4), fibronectin 1 (FN1), tissue inhibitor of metalloproteinase 1 (TIMP1). DPP4-RNA were absent in normal tissue while in cancer tissue it was detected in large amount. FN1 and TIMP1 expression were detected in normal tissue but markedly increased in papillary thyroid cancer. Among these 3 genes DPP4 seems to be the best molecular marker for papillary thyroid cancer.
Gubała
Elzbieta
E
Department of Nuclear Medicine and Endocrine Oncology, Clinic of Oncologic Surgery, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Poland.
Wiench
Małgorzata
M
Oczko-Wojciechowska
Małgorzata
M
Lange
Dariusz
D
Włoch
Jan
J
Czarniecka
Agnieszka
A
Chmielik
Ewa
E
Nikiel
Barbara
B
Wygoda
Zbigniew
Z
Kukulska
Aleksandra
A
Handkiewicz-Junak
Daria
D
Szpak-Ulczok
Sylwia
S
Krawczyk
Aleksandra
A
Roskosz
Józef
J
Jarzab
Barbara
B
D000243:Adenosine Deaminase / Q000378:metabolism; D002291:Carcinoma, Papillary / Q000235:genetics* / Q000378:metabolism; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D019715:Tissue Inhibitor of Metalloproteinase-1; C076687:CS1 peptide; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4; D000243:Adenosine Deaminase