DNA detection method based on the two-dimensional aggregation and selective desorption of nanoparticle probes.
A label-free two-dimensional colorimetric DNA sensor is reported. This sensor is based on the 2D aggregation of oligonucleotide-modified gold nanoparticle probes induced by the molecular hybridization of single-stranded oligonucleotide probes and their complementary single-stranded DNA targets. To detect the aggregation, we have developed a new detection method based on the selective desorption of nonaggregated nanoparticles. We will show here that this detection method is highly specific and allows the quantification of the DNA targets.
Charrier
Anne
A
Centre de Recherche en Matière Condensée et Nanosciences, Centre National de la Recherche Scientifique, Université de la Méditerranée, Marseille, France. charrier@crmcn.univ-mrs.fr
Candoni
Nadine
N
Thibaudau
Franck
F
D004247:DNA / Q000032:analysis*; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D015335:Molecular Probes; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D015335:Molecular Probes; D012834:Silver; D004247:DNA