title: Danazol inhibits aromatase activity of endometriosis-derived stromal cells by a competitive mechanism.
abstract: OBJECTIVE
  To evaluate the inhibitory effect of danazol on estrogen (E) production in endometriosis.
  
  
  METHODS
  Prospective randomized study.
  
  
  METHODS
  Academic research unit of the department of obstetrics and gynecology in a university hospital.
  
  
  METHODS
  Thirteen patients with endometriosis.
  
  
  METHODS
  Danazol was added to the culture of endometriosis-derived stromal cells or suspensions of microsomes prepared from chocolate cysts.
  
  
  METHODS
  The aromatase activities as well as mRNA and protein levels of aromatase in endometriosis-derived stromal cells or microsomes of endometriosis were examined.
  
  
  RESULTS
  Danazol treatment with a concentration greater than 10(-6) M significantly suppressed aromatase activity of endometriosis-derived stromal cells under basal and prostaglandin E(2) (PGE(2))-stimulated conditions. Danazol (10(-5) M) did not affect mRNA and protein levels of aromatase. Danazol competitively inhibited aromatase activity (by 1.7 x 10(-6) M of calculated Ki and 2.9 x 10(-5) M of Ki') of endometriosis microsomes.
  
  
  CONCLUSIONS
  Danazol competitively inhibited aromatase activity in endometriosis-derived stromal cells without affecting either the mRNA or protein levels of aromatase. These results indicate the efficacy of local application of danazol to endometriotic lesions.
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa, Japan. k-muraka@med.kanazawa-u.ac.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Murakami
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Nomura
authors.forename: Kazunori
authors.initials: K
authors.lastname: Shinohara
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Kasai
authors.forename: Makio
authors.initials: M
authors.lastname: Shozu
authors.forename: Masaki
authors.initials: M
authors.lastname: Inoue
mesh_terms: D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D047072:Aromatase Inhibitors / Q000008:administration & dosage* / Q000494:pharmacology; D015153:Blotting, Western; D002478:Cells, Cultured; D003613:Danazol / Q000008:administration & dosage* / Q000494:pharmacology; D004715:Endometriosis / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007700:Kinetics; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000187:drug effects / Q000201:enzymology*
chemical_list: D047072:Aromatase Inhibitors; D012333:RNA, Messenger; D001141:Aromatase; D003613:Danazol