title: Role of renal nerves and salt intake on erythropoietin secretion in rats following carbon monoxide exposure.
abstract: Because the data from the literature contain conflicting results regarding the role of renal nerves and angiotensin II in hypoxia-induced erythropoietin (EPO) secretion, we evaluated the effect of renal nerves and salt intake in rats on EPO secretion stimulated by carbon monoxide (CO). Serum levels and renal mRNA content of EPO were similarly elevated by exposure to different CO concentrations in a dose-dependent manner in rats with bilateral renal denervation (DNX) and in sham-denervated controls (INN). However, at 600 ppm CO, serum concentrations and mRNA of EPO were significantly higher in DNX compared with INN rats (p < 0.05). This increase of EPO secretion in DNX rats could be blocked by administration of neuropeptide Y (NPY) (p < 0.05), whereas the NPY receptor antagonist did not enhance EPO secretion in INN rats after CO exposure. Agonists and antagonists of beta-adrenergic receptors had no effect on EPO secretion. High-salt (HS) diet reduced EPO secretory response at 600 ppm CO by 55% compared with INN rats on normal salt diet (p < 0.01). In addition, DNX increased EPO secretion in rats on low-salt and HS diet, whereas plasma renin activity did not correlate with EPO levels under these experimental conditions. In summary, our data suggest that renal nerves contribute to the half-maximal EPO secretory response to CO exposure, possibly via NPY receptors.
authors.affiliation: Department of Pharmacology and Toxicology, University Hospital Tuebingen, D-72074 Tuebingen, Germany.
authors.forename: Cathérine
authors.initials: C
authors.lastname: Gebhard
authors.forename: Fotios
authors.initials: F
authors.lastname: Petroktistis
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Daniel
authors.initials: D
authors.lastname: Kammerer
authors.forename: Christoph
authors.initials: C
authors.lastname: Köhle
authors.forename: Karin
authors.initials: K
authors.lastname: Klingel
authors.forename: Margitta
authors.initials: M
authors.lastname: Albinus
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Gleiter
authors.forename: Hartmut
authors.initials: H
authors.lastname: Osswald
authors.forename: Almut
authors.initials: A
authors.lastname: Grenz
mesh_terms: D000818:Animals; D002248:Carbon Monoxide / Q000378:metabolism / Q000633:toxicity*; D003714:Denervation; D004921:Erythropoietin / Q000097:blood / Q000378:metabolism*; D007668:Kidney / Q000294:innervation*; D008297:Male; D009478:Neuropeptide Y / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage*
chemical_list: D009478:Neuropeptide Y; D017673:Sodium Chloride, Dietary; D004921:Erythropoietin; D002248:Carbon Monoxide