[Identification and typing of human enteroviruses using an RT-PCR assay].
BACKGROUND
To establish a molecular detection and typing assay for identification and typing of human enteroviruses (HEV) which is suitable for clinical detection and epidemiologic research.


METHODS
Using both primers specific for HEV genus and HEV typing primers and reverse transcription polymerase chain reaction (RT-PCR) the authors detected preliminarily HEV by agarose gel electrophoresis and then identified serotype through nucleotide sequence analysis of RT-PCR amplicons. The monospecific antisera neutralization was applied to validate the typing results.


RESULTS
The serotype of 18 suspicious HEV samples was identified: 4 Coxsackievirus type A24 (CVA24), 3 CVB3, 1 CVB2, 1 CVA9, 1 CVA15, 1 Echovirus type 3 (E3), 1 E6, 1 E9, 1 E11, 1 E14, 1 E33 and 1 Rhinovirus type 9. The result was validated by monospecitive antisera neutralization.


CONCLUSIONS
This RT-PCR assay for HEV detection and typing may be suitable for clinical detection and epidemic research since this method is sensitive and specific for direct identification and typing of HEV.
Hou
Wen-jun
WJ
Postgraduate Medical School, The People's Liberation Army, Beijing 100039, China.
Yang
Jian-yang
JY
Hu
Yan
Y
Sun
Ying
Y
Mao
Pan-yong
PY
D017931:DNA Primers; D004770:Enterovirus / Q000145:classification / Q000235:genetics*; D004769:Enterovirus Infections / Q000175:diagnosis*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods
D017931:DNA Primers; D012367:RNA, Viral