Comparative proteomic analysis between the invasive and commensal strains of Staphylococcus epidermidis.
Staphylococcus epidermidis is one of the major causative agents for nosocomial infections. To reveal the pathogenesis factors, we performed the comparative proteomic analysis of invasive ATCC35984 and commensal ATCC12228 strains by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. The differentially expressed proteins were involved in carbohydrate metabolism, sugar binding, lipid degradation and amino acid binding. In addition, we demonstrated that the trap gene was transcribed by 3.657+/-0.156 (P<0.01) -fold higher in ATCC35984 than in ATCC12228. Levels of accumulation-associated protein (AAP) were found to be low by the immuno-dot blotting assay in ATCC12228, which is unable to form biofilm. Our results suggest that the target of RNAIII activating protein and AAP may contribute to S. epidermidis virulence and biofilm formation.
Yang
Xiao-Mei
XM
Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.
Li
Na
N
Chen
Jie-Min
JM
Ou
Yuan-Zhu
YZ
Jin
Hong
H
Lu
Hao-Jie
HJ
Zhu
Yu-Li
YL
Qin
Zhi-Qiang
ZQ
Qu
Di
D
Yang
Peng-Yuan
PY
D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018441:Biofilms / Q000254:growth & development; D050260:Carbohydrate Metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D050356:Lipid Metabolism; D040901:Proteomics / Q000379:methods; D016601:RNA-Binding Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013212:Staphylococcus epidermidis / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; C079092:MtrB protein, Bacteria; D016601:RNA-Binding Proteins; D014157:Transcription Factors