title: Lactobacillus salivarius CECT 5713, a potential probiotic strain isolated from infant feces and breast milk of a mother-child pair.
abstract: In this study, Lactobacillus salivarius CECT 5713 was originally isolated from feces of a one-month-old breast-fed infant. Since it has been suggested that the gut microbiota of breast-fed infants reflects that of the maternal breast milk, we investigated if this specific strain was present in breast milk of the respective mother. RAPD and PFGE analysis revealed the presence of the strain L. salivarius CECT 5713 in this biological fluid. To our knowledge, this is the first report of a L. salivarius strain isolated from breast milk. L. salivarius CECT 5713 produced l-lactate, acetate and hydrogen peroxide, which may be responsible for its antimicrobial activity against most of the indicator organisms used in this study; in addition, this strain showed a high survival rate after exposition to conditions simulating those found in the gastrointestinal tract. Finally, it was strongly adhesive to Caco-2 and HT-29 cells did not produce biogenic amines and were unable to degrade gastric mucin in vitro.
authors.affiliation: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Martín
authors.forename: E
authors.initials: E
authors.lastname: Jiménez
authors.forename: M
authors.initials: M
authors.lastname: Olivares
authors.forename: M L
authors.initials: ML
authors.lastname: Marín
authors.forename: L
authors.initials: L
authors.lastname: Fernández
authors.forename: J
authors.initials: J
authors.lastname: Xaus
authors.forename: J M
authors.initials: JM
authors.lastname: Rodríguez
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000328:Adult; D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D015169:Colony Count, Microbial; D005243:Feces / Q000382:microbiology*; D005260:Female; D005752:Gastric Mucins / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007231:Infant, Newborn; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology*; D008895:Milk, Human / Q000382:microbiology*; D019936:Probiotics
chemical_list: D005752:Gastric Mucins; D019344:Lactic Acid; D019342:Acetic Acid