title: Halorubrum ezzemoulense sp. nov., a halophilic archaeon isolated from Ezzemoul sabkha, Algeria.
abstract: A novel extremely halophilic archaeon was isolated from Ezzemoul sabkha, Algeria. The strain, designated 5.1(T), was neutrophilic, motile and Gram-negative. At least 15 % (w/v) NaCl was required for growth. The isolate grew at pH 6.5-9.0, with optimum growth at pH 7.0-7.5. Mg(2+) was required for growth. Polar lipids were C(20)C(20) derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, and phosphatidylglycerol sulfate and sulfated diglycosyl diether. The genomic DNA G+C content of strain 5.1(T) was 61.9 mol% (T(m)). Phylogenetic analysis based on comparison of 16S rRNA gene sequences revealed that strain 5.1(T) clustered with Halorubrum species. The results of DNA-DNA hybridization and biochemical tests allowed genotypic and phenotypic differentiation of strain 5.1(T) from other Halorubrum species. The name Halorubrum ezzemoulense sp. nov. (type strain 5.1(T)=CECT 7099(T)=DSM 17463(T)) is proposed.
authors.affiliation: Institut de Nutrition de l'Alimentation et des Technologies Agro-Alimentaires, Université Mentouri, Constantine, Algeria.|Departamento de Microbiologia, Facultad de Farmacia, Campus Universitario de Cartuja s/n 18071, Granada, Spain.
authors.forename: Karima
authors.initials: K
authors.lastname: Kharroub
authors.affiliation: Departamento de Microbiologia, Facultad de Farmacia, Campus Universitario de Cartuja s/n 18071, Granada, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Quesada
authors.affiliation: Departamento de Microbiologia, Facultad de Farmacia, Campus Universitario de Cartuja s/n 18071, Granada, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Ferrer
authors.affiliation: Departamento de Microbiologia, Facultad de Farmacia, Campus Universitario de Cartuja s/n 18071, Granada, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Fuentes
authors.affiliation: Departamento de Microbiologia, Facultad de Farmacia, Campus Universitario de Cartuja s/n 18071, Granada, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Aguilera
authors.affiliation: Institut des Sciences de la Nature, Faculté des Sciences, Université Mentouri, Constantine, Algeria.
authors.forename: Abdrahmane
authors.initials: A
authors.lastname: Boulahrouf
authors.affiliation: Departamento de Microbiologia, Facultad de Farmacia, Campus Universitario de Cartuja s/n 18071, Granada, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Ramos-Cormenzana
authors.affiliation: Departamento de Microbiologia, Facultad de Farmacia, Campus Universitario de Cartuja s/n 18071, Granada, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Monteoliva-Sánchez
mesh_terms: D000462:Algeria; D015373:Bacterial Typing Techniques; D001482:Base Composition; D019641:DNA, Archaeal / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D006216:Halobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009068:Movement; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology*; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D008055:Lipids; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride