title: Culture-independent molecular subtyping of Mycoplasma pneumoniae in clinical samples.
abstract: A new molecular subtyping approach was developed which is based on the amplification and sequencing of a repetitive region of the P1 gene of Mycoplasma pneumoniae. It allows the differentiation of all known subtypes and variants of M. pneumoniae as well as the identification of new subtypes directly in clinical samples to characterize endemic and epidemic M. pneumoniae infections.
authors.affiliation: Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany. roger.dumke@mailbox.tu-dresden.de
authors.forename: Roger
authors.initials: R
authors.lastname: Dumke
authors.forename: Paul Christian
authors.initials: PC
authors.lastname: Lück
authors.forename: Christoph
authors.initials: C
authors.lastname: Noppen
authors.forename: Christoph
authors.initials: C
authors.lastname: Schaefer
authors.forename: Heike
authors.initials: H
authors.lastname: von Baum
authors.forename: Reinhard
authors.initials: R
authors.lastname: Marre
authors.forename: Enno
authors.initials: E
authors.lastname: Jacobs
mesh_terms: D018829:Adhesins, Bacterial / Q000145:classification / Q000235:genetics; D000595:Amino Acid Sequence; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods*; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D011019:Pneumonia, Mycoplasma / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
chemical_list: D018829:Adhesins, Bacterial; D004269:DNA, Bacterial; C035471:adhesin, Mycoplasma pneumoniae