title: Gynogenic plant regeneration from unpollinated flower explants of Eragrostis tef (Zuccagni) Trotter.
abstract: Tef [Eragrostis tef (Zucc.) Trotter] is the most important cereal in Ethiopia. In its wild relative E. mexicana, regeneration of six green plants resulted from culture of 121 non-pollinated immature pistils. In the allotetraploid crop species tef, however, only callus and root formation was obtained by this method. By contrast, immature spikelets and panicle segments of E. tef proved amenable to gynogenic plant regeneration. Upon step-wise optimization of the protocol, efficient plant formation was achieved in all three cultivars tested. In cv. DZ-01-196, culture of 1305 immature spikelets resulted in formation of 159 green plants. Flow cytometric analysis revealed (di)haploid, triploid, tetraploid and octoploid regenerants, from which the vast majority was tetraploid. Tef-breeding programs will likely benefit substantially from efficient generation of true-breeding plants.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466, Gatersleben, Germany.
authors.forename: Likyelesh
authors.initials: L
authors.lastname: Gugsa
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Sarial
authors.forename: Horst
authors.initials: H
authors.lastname: Lörz
authors.forename: Jochen
authors.initials: J
authors.lastname: Kumlehn
mesh_terms: D003080:Cold Temperature; D031750:Eragrostis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005434:Flow Cytometry; D035264:Flowers / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D018745:Genome, Plant; D010937:Plant Growth Regulators / Q000494:pharmacology; D011003:Ploidies; D011058:Pollen / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects; D046509:Tissue Culture Techniques
chemical_list: D010937:Plant Growth Regulators