title: Transient ischaemia affects plasma membrane glutamate transporter, not vesicular glutamate transporter, expressions in the gerbil hippocampus.
abstract: In the present study, we investigated expressions of vesicular glutamate transporter (VGLUT) and of the plasma membrane glutamate transporters [glutamate transporter 1 (GLT-1), glutamate/aspartate transporter (GLAST) and excitatory amino acid carrier 1 (EAAC-1)] in the gerbil hippocampus following transient ischaemia. The expressional levels and distribution patterns of VGLUT immunoreactivities were unaltered until 3 days after ischaemic-insults. However, VGLUT-2 immunoreactivity in the CA1 region was reduced at 4 days after ischaemia due to delayed neuronal death. In addition, both GLT-1 and GLAST immunoreactivities in the CA1 region were enhanced at 30 min - 12 h after ischaemia-reperfusion and their expression began to reduce at 24 h after ischaemia-reperfusion. In contrast, EAAC-1 immunoreactivity was transiently reduced in the CA1 region at 30 min after ischaemia, re-enhanced at 3-12 h after ischaemia, and re-reduced at 24 h after ischaemia. These findings suggest that malfunctions of plasma membrane glutamate transporters, not of VGLUT, may play an important role in the elevation of extracellular glutamate concentration following ischaemic insults.
authors.affiliation: Department of Anatomy, College of Medicine, Hallym University, Chunchon, South Korea.
authors.forename: D-S
authors.initials: DS
authors.lastname: Kim
authors.forename: S-E
authors.initials: SE
authors.lastname: Kwak
authors.forename: J-E
authors.initials: JE
authors.lastname: Kim
authors.forename: J-Y
authors.initials: JY
authors.lastname: Jung
authors.forename: M H
authors.initials: MH
authors.lastname: Won
authors.forename: S-Y
authors.initials: SY
authors.lastname: Choi
authors.forename: O-S
authors.initials: OS
authors.lastname: Kwon
authors.forename: T-C
authors.initials: TC
authors.lastname: Kang
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000378:metabolism; D000818:Animals; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism; D005849:Gerbillinae; D050491:Glutamate Plasma Membrane Transport Proteins / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D050598:Vesicular Glutamate Transport Protein 1 / Q000378:metabolism*
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D027341:Excitatory Amino Acid Transporter 1; D050491:Glutamate Plasma Membrane Transport Proteins; D050598:Vesicular Glutamate Transport Protein 1