Paracoccus bengalensis sp. nov., a novel sulfur-oxidizing chemolithoautotroph from the rhizospheric soil of an Indian tropical leguminous plant.
Paracoccus versutus-like isolates from the rhizosphere of Clitoria ternatea, a slender leguminous herb (family--Papilionaceae), found ubiquitously in waste places and village forests of the Lower Gangetic plains of India, presented a case of graduated infraspecific variation that was capped by the identification of a new species Paracoccus bengalensis (type strain JJJ(T) = LMG 22700(T) = MTCC 7003(T)). The diverged phenetic and genetic structure of these sulfur-oxidizing chemolithoautotrophs presented a case of apparent nonconformity of 16S rRNA gene sequence similarities with results of DNA-DNA hybridization. Despite high 16S rRNA gene sequence similarity with P. versutus one of the newly isolated strains, viz., JJJ(T) was identified as a new species of Paracoccus by virtue of its explicitly low DNA-DNA hybridization (42-45%) with the type strain of the closest species P. versutus (), distinct G + C content (65.3 mol%), physiological and biochemical differences amounting to <60% phenetic similarity with strains of P. versutus as well as new isolates akin to the species. The newly described species also had a unique fatty acid profile that was distinguished by the absence of 18:1 omega9c, unique possession of Summed feature 3 (16:1omega7c & 15:0 iso 2-OH), 19:0 10 methyl, and a much higher concentration of 19:0 cycloomega8c.
Ghosh
Wriddhiman
W
Department of Microbiology, Bose Institute, P-1/12, C.I.T. Scheme VII-M, Kolkata 700 054, India. Wriman@rediffmail.com
Mandal
Sukhendu
S
Roy
Pradosh
P
D007887:Fabaceae / Q000382:microbiology; D005227:Fatty Acids / Q000032:analysis; D010230:Paracoccus / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D027343:Rhizome / Q000382:microbiology; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013455:Sulfur / Q000378:metabolism; D013776:Tetrathionic Acid / Q000378:metabolism
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D013455:Sulfur; D013776:Tetrathionic Acid