title: Cellular gene expression survey of PseudoRabies Virus (PRV) infected Human Embryonic Kidney cells (HEK-293).
abstract: Pseudorabies virus (PRV) is an alpha herpesvirus that causes Aujezsky disease in the pig. To characterize the impact of PRV infection on cellular expression, we used microarrays consisting of 9850 oligonucleotides corresponding to human genes and examined the expression levels of mRNA isolated 0.5, 3, 6, and 9 h post infection (hpi) from cultures of infected HEK-293 cells. Very few changes were observed during the first 3 h of infection but significant modifications in the cell expression of more than 1000 genes were clearly apparent by 6 hpi. More than 2400 genes were either up- or down-regulated during the 9 h experiment. These results were then analyzed using gene ontology and the MAPP and MAPPFinder software. This comprehensive analysis clearly shows that the down-regulated genes were mainly involved in macromolecular synthesis (DNA, RNA and proteins) and the cell cycle. The up-regulated genes primarily concerned the regulation of DNA transcription, developmental processes (central nervous system development, neurogenesis, angiogenesis), cell adhesion and potassium transport. This study is the first qualitative analysis of a gene expression survey in a human cell line following PRV infection. It demonstrates global changes in the cell expression profile, and identifies the main biological processes that are altered during virus replication.
authors.affiliation: Laboratoire de Génétique Virale et Biosécurité, Agence Française de Sécurité Sanitaire des Aliments, BP 53, 22440 Ploufragan, France. y.blanchard@afssa.fr
authors.forename: Yannick
authors.initials: Y
authors.lastname: Blanchard
authors.forename: Nolwenn
authors.initials: N
authors.lastname: Le Meur
authors.forename: Martine
authors.initials: M
authors.lastname: Le Cunff
authors.forename: Philippe
authors.initials: P
authors.lastname: Blanchard
authors.forename: Jean
authors.initials: J
authors.lastname: Léger
authors.forename: André
authors.initials: A
authors.lastname: Jestin
mesh_terms: D000818:Animals; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D011558:Herpesvirus 1, Suid / Q000378:metabolism* / Q000472:pathogenicity; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary*; D011557:Pseudorabies / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000032:analysis* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013997:Time Factors; D015854:Up-Regulation; D014779:Virus Replication
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral