title: PRE-mediated bypass of two Su(Hw) insulators targets PcG proteins to a downstream promoter.
abstract: Drosophila Polycomb group response elements (PRE) silence neighboring genes, but silencing can be blocked by one copy of the Su(Hw) insulator element. We show here that Polycomb group (PcG) proteins can spread from a PRE in the flanking chromatin region and that PRE blocking depends on a physical barrier established by the insulator to PcG protein spreading. On the other hand, PRE-mediated silencing can bypass two Su(Hw) insulators to repress a downstream reporter gene. Strikingly, insulator bypass involves targeting of PcG proteins to the downstream promoter, while they are completely excluded from the intervening insulated domain. This shows that PRE-dependent silencing is compatible with looping of the PRE in order to bring PcG proteins in contact with the promoter and does not require the coating of the whole chromatin domain between PRE and promoter.
authors.affiliation: Institute of Human Genetics, Centre National de la Recherche Scientifique, 141 rue de la Cardonille, F-34396 Montpellier Cedex 5, France.
authors.forename: Itys
authors.initials: I
authors.lastname: Comet
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Savitskaya
authors.forename: Bernd
authors.initials: B
authors.lastname: Schuettengruber
authors.forename: Nicolas
authors.initials: N
authors.lastname: Nègre
authors.forename: Sergey
authors.initials: S
authors.lastname: Lavrov
authors.forename: Aleksander
authors.initials: A
authors.lastname: Parshikov
authors.forename: François
authors.initials: F
authors.lastname: Juge
authors.forename: Elena
authors.initials: E
authors.lastname: Gracheva
authors.forename: Pavel
authors.initials: P
authors.lastname: Georgiev
authors.forename: Giacomo
authors.initials: G
authors.lastname: Cavalli
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001665:Binding Sites / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D020868:Gene Silencing; D017344:Genes, Insect; D008954:Models, Biological; D063150:Polycomb Repressive Complex 1; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C066770:Pc protein, Drosophila; D012097:Repressor Proteins; C083738:su(Hw) protein, Drosophila; D063150:Polycomb Repressive Complex 1