title: The effect of acteoside on histamine release and arachidonic acid release in RBL-2H3 mast cells.
abstract: The effect of acteoside, a phenylpropanoid glycoside isolated from Clerodendron trichotomum Thunberg, on histamine and arachidonic acid release was investigated in RBL 2H3 cells. Histamine was dose-dependently released from RBL 2H3 cells by melittin, arachidonic acid and thapsigargin. In extracellular Ca2+-free solution, basal secretion of histamine increased by two fold. The response of histamine release to melittin and thapsigargin in Ca2+-free solution was significantly decreased, whereas the response to arachidonic acid was significantly increased as compared with those in normal solution. Acteoside inhibited histamine release induced by melittin, arachidonic acid and thapsigargin in a dose-dependent manner in the presence or absence of extracellular Ca2+. However, the inhibitory activity of acteoside was more potent in normal solution than that in Ca2+-free solution. These data suggest that inhibitory mechanism of acteoside on histamine release may be related to extracellular Ca2+. On the other hand, acteoside significantly inhibited arachidonic acid release and prostaglandin E2 production induced by 0.5 microM melittin. It is possible that acteoside may be developed as an anti-inflammatory agent.
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Ji Yun
authors.initials: JY
authors.lastname: Lee
authors.forename: Hyo Suk
authors.initials: HS
authors.lastname: Kang
authors.forename: Chan Hun
authors.initials: CH
authors.lastname: Jeong
authors.forename: Hee
authors.initials: H
authors.lastname: Moon
authors.forename: Wan Kyunn
authors.initials: WK
authors.lastname: Whang
authors.forename: Chang Jong
authors.initials: CJ
authors.lastname: Kim
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Sim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism; D045744:Cell Line, Tumor; D032409:Clerodendrum / Q000737:chemistry*; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D020536:Enzyme Activators / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006636:Histamine Release; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology; D008555:Melitten / Q000494:pharmacology; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010741:Phospholipases A / Q000378:metabolism; D051381:Rats; D019284:Thapsigargin / Q000494:pharmacology; D059808:Polyphenols
chemical_list: D000893:Anti-Inflammatory Agents; D016718:Arachidonic Acid; D002118:Calcium; D000252:Calcium-Transporting ATPases; D015232:Dinoprostone; D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D005960:Glucosides; D008555:Melitten; D010636:Phenols; D010741:Phospholipases A; D019284:Thapsigargin; C058956:acteoside; D059808:Polyphenols