title: Effects of two infectious bursal disease vaccine virus strains on hepatic microsomal enzyme activities in chickens.
abstract: The influence of two infectious bursal disease vaccines on the activities of hepatic microsomal enzymes aniline hydroxylase, ethylmorphine N-demethylase, NADPH-cytochrome c reductase, aryl sulphotransferase and p-nitrophenol UDP-glucuronyltransferase was investigated in chickens. The vaccines contained attenuated Winterfield 2512 and VMG-91 strains, respectively. The activities of enzymes were determined on postvaccination days 0, 2, 5 and 7. At the same time, post-mitochondrial supernatant, cytosolic and microsomal pellet protein concentrations were determined. As expected, the antibody titres against infectious bursal disease virus in the serum were increased in both tested groups in relation to each administered vaccine. Using RT-PCR, the presence of the VP2 gene fragment of virus in the liver of chicken was demonstrated 4 and 6 h after vaccination. The results of this study suggest that the two commercial vaccines modulate the activities of five enzymes tested, and that the two attenuated vaccines applied triggered induction and/or inhibition of phases I and II of biotransformation enzyme activities.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia. apcrnic@vef.hr
authors.forename: Andreja Prevendar
authors.initials: AP
authors.lastname: Crnić
authors.forename: D
authors.initials: D
authors.lastname: Sakar
authors.forename: Jelena
authors.initials: J
authors.lastname: Pompe-Gotal
authors.forename: Z
authors.initials: Z
authors.lastname: Bidin
authors.forename: Ivana
authors.initials: I
authors.lastname: Lojkić
authors.forename: S
authors.initials: S
authors.lastname: Cajavec
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D018175:Birnaviridae Infections / Q000201:enzymology / Q000517:prevention & control / Q000662:veterinary*; D002645:Chickens; D005260:Female; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000472:pathogenicity; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D011201:Poultry Diseases / Q000201:enzymology / Q000517:prevention & control*; D011897:Random Allocation; D014611:Vaccination / Q000379:methods / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000494:pharmacology; D014765:Viral Vaccines / Q000494:pharmacology*
chemical_list: D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines