Assessment of bone mineral and matrix using backscatter electron imaging and FTIR imaging.
The resistance of bone to fracture is determined by its geometric and material properties. The geometry and density can be determined by radiographic methods, but material properties such as collagen structure, mineral composition, and crystal structure currently require analysis by invasive techniques. Backscatter electron imaging provides quantitative information on the distribution of the mineral within tissue sections, and infrared and other vibrational spectroscopic methods can supplement these data, providing site-specific information on mineral content as well as information on collagen maturity and distributions of crystal size and composition. This information contributes to the knowledge of "bone quality."
Boskey
Adele L
AL
Hospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USA. Boskeya@hss.edu
D015519:Bone Density / Q000502:physiology*; D001857:Bone Matrix / Q000502:physiology*; D004583:Electrons; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D046032:Microscopy, Energy-Filtering Transmission Electron; D010024:Osteoporosis / Q000175:diagnosis / Q000503:physiopathology; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman