Detection of infectious poxvirus particles.
To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect <3 PFU of infectious poxvirus particles in <5 hours. This approach involved virus culture combined with real-time reverse transcription-polymerase chain reaction detection of 2 viral genes expressed immediately after infection.
Nitsche
Andreas
A
Robert Koch-Institut, Berlin, Germany. nitschea@rki.de
Stern
Daniel
D
Ellerbrok
Heinz
H
Pauli
Georg
G
D045744:Cell Line, Tumor; D004279:DNA, Viral / Q000302:isolation & purification; D015967:Gene Expression Regulation, Viral; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014616:Vaccinia virus / Q000302:isolation & purification*; D014779:Virus Replication
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral