Matrix metalloproteinase-9 in broncho-alveolar lavage fluid of patients with non-small cell lung cancer.
OBJECTIVE
To evaluate concentration of MMP-9 in blood plasma and broncho-alveolar lavage fluid (BALF) from patients with non-small cell lung cancer (NSCLC).


METHODS
Blood plasma from 40 NSCLC patients and 40 healthy donors was collected and concentrations of blood plasma and BALF MMP-9 were measured using ELISA. Correlation between MMP-9 level and gender, histological type of tumor and stage of disease was analyzed.


RESULTS
Levels of blood plasma MMP-9 were significantly higher in NSCLC patients (p < 0.0001) then in control group, and were especially high in patients with stage IV of disease (stage I vs stage IV - p < 0.005, stage II vs stage IV - p < 0.01, stage III vs stage IV - p < 0.01). Also, stage IV of NSCLC was characterized by the highest level of BALF MMP-9 (stage I vs stage IV - p < 0.002, stage II vs stage IV p < 0.002, and stage III vs stage IV p < 0.007). Correlation between blood plasma and BALF MMP-9 levels and gender or histological type of tumor was insignificant.


CONCLUSIONS
Our data revealed significant correlation between tumor stage and BALF and plasma MMP-9 levels in NSCLC patients.
Bugdayci
G
G
Department of Biochemistry, Izzet Baysal School of Medicine, Abant Izzet Baysal University, Bolu, Turkey. gbugdayci@gmail.com
Kaplan
T
T
Sezer
S
S
Turhan
T
T
Koca
Y
Y
Kocer
B
B
Yildirim
E
E
D000328:Adult; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000032:analysis* / Q000097:blood; D008875:Middle Aged; D009367:Neoplasm Staging
D020780:Matrix Metalloproteinase 9