Pax-5 immunoexpression in various types of benign and malignant tumours: a high-throughput tissue microarray analysis.
BACKGROUND
Pax-5 belongs to the Pax gene family transcription factors that play an important role in organogenesis and in B cell ontogeny. It is expressed in B cell non-Hodgkin's lymphoma (B-NHL), Hodgkin's lymphoma (HL) and neuroendocrine carcinomas. However, its expression in other tumour types is not fully explored.


OBJECTIVE
To determine Pax-5 expression in other tumour types, immunohistochemistry was performed on 3758 benign and malignant tumours using multiple tumour microarrays, as well as on whole sections.


RESULTS
Pax-5 was expressed in 108/118 (91.5%) B-NHLs, in 60/70 (85.7%) HLs and in 0/7 T cell lymphomas. In addition, Pax-5 was seen in 24/34 (70.6%) Merkel cell carcinomas, 42/53 (79.2%) small cell carcinomas, 1/164 (0.6%) breast carcinomas, 2/204 (1%) endometrial adenocarcinomas and 1/452 (0.2%) urothelial carcinoma of the bladder.


CONCLUSIONS
Despite its expression in a small subset of malignancies of epithelial origin, Pax-5 is still a good and reliable immunomarker in diagnosing B-NHL, HL and neuroendocrine carcinomas.
Mhawech-Fauceglia
Paulette
P
Department of Pathology and Laboratory Medicine, Roswell Park Cancer Institute, Buffalo, New York 14263, USA. pmhawech1@yahoo.com
Saxena
Rhakee
R
Zhang
Shaozeng
S
Terracciano
Luigi
L
Sauter
Guido
G
Chadhuri
Arundhuti
A
Herrmann
Francois R
FR
Penetrante
Remedios
R
D014408:Biomarkers, Tumor / Q000378:metabolism*; D015266:Carcinoma, Merkel Cell / Q000175:diagnosis / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism; D003937:Diagnosis, Differential; D006689:Hodgkin Disease / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000175:diagnosis / Q000378:metabolism; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D051757:PAX5 Transcription Factor / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human