A 3 kDa peptide is involved in the chemoattraction in vitro of the male Schistosoma mansoni to the female.
In vitro experiments on adult Schistosoma mansoni worms have shown the existence of mutual attraction between males and females mediated by factor(s) released by both sexes. Males were attracted to female-released factor(s), which was found in <30 kDa fractions. A combination of bioassays, size fractionations, chromatographic and chemical and enzymatic treatments were used to identify female-released factor(s) that attracted males. Boiling reduced the attraction of males towards female incubate. Male-attractant factor(s) was not in the female vomitus, and TLC showed the absence of lipids and carbohydrates in the female incubate. Male-attractant factor(s) was found to be sensitive to proteases and to be hydrophilic in nature. Using RP-HPLC, a 3260 Da peptide released by S. mansoni adult females that plays a role in attracting the males was identified, purified and partially characterized. A partial amino acid sequence <QVHHQK> of that peptide was obtained using MALDI peptide-MS and MALDI-TOF/TOF.
Ghaleb
Amr M
AM
Division of Digestive Diseases, R275, WBRB, Emory University School of Medicine, 615 Michael St., Atlanta, GA 30322, USA.
Atwood
James
J
Morales-Montor
Jorge
J
Damian
Raymond T
RT
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D012550:Schistosoma mansoni / Q000737:chemistry* / Q000502:physiology; D012724:Sex Attractants / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D010455:Peptides; D012724:Sex Attractants