title: Comparison between immunofluorescence and PCR in detecting human papilloma virus in condyloma acuminata.
abstract: OBJECTIVE
  To compare the effectiveness of immunofluorescence and polymerase chain reaction (PCR) in detecting human papilloma virus (HPV) in condyloma acuminata (CA).
  
  
  METHODS
  HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism (RFLP).
  
  
  RESULTS
  The positive detective rates of immunofluorescence and PCR were 56.67% (34/60) and 96.67% (58/ 60), respectively (P < 0.01). RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus, which accounted for 98.28%.
  
  
  CONCLUSIONS
  The sensibility of PCR is superior to that of immunofluorescence.
authors.affiliation: Department of Dermatology, Beijing Chaoyang Hospital, Beijing 100020. roseliufang@sohu.com
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Jia-Bi
authors.initials: JB
authors.lastname: Wang
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Liu
authors.forename: Ya-Gang
authors.initials: YG
authors.lastname: Zuo
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Man
mesh_terms: D003218:Condylomata Acuminata / Q000821:virology*; D005260:Female; D052140:Human papillomavirus 11 / Q000235:genetics / Q000302:isolation & purification; D052163:Human papillomavirus 6 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction