A (4R)-hydroxy-L-proline-derived chiral scaffold and its oligomers as chiral selectors in liquid chromatography chiral stationary phases for enantioseparation.
The chromatographic behaviour of a poly-L-proline-derived chiral stationary phase (CSP) is compared to the corresponding single proline-derived CSP. Structurally diverse racemic test compounds and mobile phases, including normal- and RP conditions, were used. Although the application domain of the poly-L-proline-derived CSP (CSP-3) was considerably restricted, this CSP showed a higher retention and a slightly broader application domain than the monomeric analogue (CSP-1) when heptane/2-PrOH was used as mobile phase. The presence of an alcohol in the mobile phase was essential for enantioseparation in the poly-L-proline-derived CSP when normal-phase conditions were applied.
Sancho
Raquel
R
Institut de Recerca Biomèdica, Parc Científic de Barcelona (IRB-PCB), Josep Samitier, Barcelona, Spain.
Pérez
Anna M
AM
Minguillón
Cristina
C
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006909:Hydroxyproline / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism
D009842:Oligopeptides; D010455:Peptides; D004364:Pharmaceutical Preparations; C011083:polyproline; D006909:Hydroxyproline