title: [Dependence of Ca2+ on the acetylcholine-sensitive current in guinea pig type II vestibular hair cells].
abstract: OBJECTIVE
  To explore the dependence of Ca2+ on the acetylcholine (ACh)-sensitive potassium current in guinea pig type II vestibular hair cells.
  
  
  METHODS
  Under the whole-cell patch mode, the current amplitude of the ACh-sensitive potassium current was recorded in response to the concentration change of the extracellular or intracellular Ca2+.
  
  
  RESULTS
  Following application of ACh, type II vestibular hair cells displayed the sustained potassium current, which was inhibited by tetraethylammonium chloride (TEA), but not inhibited by 4-aminopyrine (4-AP). The activation of the ACh-sensitive potassium current was strongly affected by the concentration of the extracellular Ca2+. The current amplitude of the ACh-sensitive potassium increased following the increase of Ca2+ concentration from 0 mmol/L to 4 mmol/L At the concentration of 4 mmol/L Ca2+, the current amplitude of the ACh-sensitive potassium current reached the maximal response. Lowering the Ca2 concentration in the external solution from 4 mmol/L to 0. 5 mmol/L, the current amplitude of the ACh-sensitive potassium current was inhibited to (36.5 +/- 6.5)%. However, no difference was found in the presence and in the absence of the intracellular heparin, which was a well-known blocker of the inositol trisphosphate-dependent calcium release channels. In addition, the calcium channel blocker, Cd2+, inhibited the ACh-sensitive potassium current.
  
  
  CONCLUSIONS
  The activation of the ACh-sensitive potassium current in guinea pig type II vestibular hair cells was dependent on the extracellular Ca2+ influx through the calcium channel. The application of ACh would stimulate membrane Ca2+ channels; the influx of Ca2+ will then activate the calcium-dependent potassium current in guinea pig type II hair cells to mediate the hyperpolarization effect.
authors.affiliation: Department of Otorhinolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Chang-kai
authors.initials: CK
authors.lastname: Guo
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.forename: Wei-jia
authors.initials: WJ
authors.lastname: Kong
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Qing-tian
authors.initials: QT
authors.lastname: Li
mesh_terms: D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism*; D006168:Guinea Pigs; D018069:Hair Cells, Vestibular / Q000378:metabolism* / Q000502:physiology*; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D000109:Acetylcholine; D002118:Calcium