title: DNA microarray analysis of changes in gene expression induced by 1,25-dihydroxyvitamin D3 in human promyelocytic leukemia HL-60 cells.
abstract: Using a DNA microarray, we analyzed about 16,600 genes for changes in expression associated with the differentiation of human promyelocytic leukemia HL-60 cells induced by 1alpha,25-dihydroxyvitamin D3 (DVD). Many of the up-regulated genes could be correlated to differentiation-associated changes toward a monocyte/macrophage lineage, and many down-regulated genes could be correlated to repressed cell growth. The present study revealed the down-regulated gene expression of importins and exportins 1, 5, 7, and exportin-tRNA. Thus, the present results confirmed our previous findings of down-regulation of exportin 1 and exportin-tRNA by DVD. Gene expression of exportin 6 is suggested to be regulated differently from that of exportins 1, 5, 7, and exportin-tRNA. The down-regulation of nuclear transport factors may be deeply associated with the differentiation of HL-60 cells induced by DVD.
authors.affiliation: Laboratory of Cellular Biochemistry, Graduate School of Nutritional and Environmental Sciences, and COE for the 21th Century, University of Shizuoka, Yada, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tazoe
authors.forename: Kyoko
authors.initials: K
authors.lastname: Taguchi
authors.forename: Yu
authors.initials: Y
authors.lastname: Koyama
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Sumio
authors.initials: S
authors.lastname: Hayakawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Munakata
authors.forename: Mamoru
authors.initials: M
authors.lastname: Isemura
mesh_terms: D002117:Calcitriol / Q000494:pharmacology*; D049109:Cell Proliferation; D017931:DNA Primers / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D028884:Karyopherins / Q000096:biosynthesis / Q000378:metabolism; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D028884:Karyopherins; D002117:Calcitriol