Effect of coverage density and structure of chemically bonded silica stationary phases on the separation of compounds with various properties.
The chemical character, geometry, and architecture of chemically formed surface layers determine interactions between stationary phase, analyte, and mobile phase, and therefore the retention mechanisms (partitioning, adsorption, ion exchange, steric exclusion) of separated analytes. These interactions also depend on the structure and chemical character of the solutes and the composition of the mobile phase. High-molecular-weight fullerenes (C60 and C70) and water-soluble selenium-containing peptides (833 and 2607 Da) were used for the evaluation of laboratory-prepared octadecyl stationary phases with high and low coverage density before and after end-capping. The aim of this work was to study differences in surface coverage density and homogeneity and conformational changes of chemically bonded moieties and the influence of these parameters on the separation of mixtures of selenopeptides and fullerenes with significantly different molecular masses. A topographical model of the chemically modified stationary surface is presented.
Buszewski
Bogusław
B
Department of Environmental Chemistry and Ecoanalytics, Faculty of Chemistry, Nicholas Copernicus University, 7 Gagarin St., 87 100 Toruń, Poland. bbusz@chem.uni.torun.pl
Krupczyńska
Katarzyna
K
Rychlicki
Gerhard
G
Lobiński
Ryszard
R
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D037741:Fullerenes / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D051140:Selenoproteins / Q000737:chemistry / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry*; D013816:Thermodynamics
D037741:Fullerenes; D051140:Selenoproteins; C069836:fullerene C70; D012822:Silicon Dioxide; C069837:fullerene C60