[Experimental studies of human adipose tissue-derived stromal cells transfected with ad-hBMP-2 gene].
OBJECTIVE
To investigate the possibility of adipose-derived stromal cells (ADSCs) transfeced by adenovirus containing human bone morphogenetic protein-2 (Ad-hBMP-2) gene and their osteogenic potential.


METHODS
ADSCs were obtained from inguinal fat tissue of 4 weeks old SD rats. After exposure to adenovirus containing green fluorescent protein(Ad-GFP), fluorescent microscope was used to observe gene transfection effect once 12 hours. After transfected with Ad-hBMP-2, cytochemistry, immmucytochemistry and Western blot were used to examine the expression of alkaline phosphatase (ALP), osteocalcin (OC) and hBMP-2.


RESULTS
After exposed to Ad-GFP 12 hours, 52% ADSCs were observed being transfected and 48 hours later reached 95%. The double number time belonged after transfecting with Ad-hBMP-2, and cytochemistry, immucytochemistry and Western blot examines indicated positive results of ALP, OC, hBMP-2 after 48 hours.


CONCLUSIONS
Adipose tissue contains abundant ADSCs which could be transfected as gene vectors by adenovirus, ADSCs transfected with Ad-hBMP-2 can convert to ostoeblasts, and can act as a kind of seed cells for osteo-tissue engineering.
Zheng
Pei-Hui
PH
Dept. of Oral and Maxillofacial Surgery, Shandong Provincial Hospital, Shandong University, Jinan 250021, China.
Wei
Feng-Cai
FC
Jin
Guo-Ying
GY
Shan
Xiu-Li
XL
Sun
Shu-Yang
SY
D000256:Adenoviridae; D017667:Adipocytes; D000273:Adipose Tissue; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D002478:Cells, Cultured; D005822:Genetic Vectors; D006801:Humans; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells; D023822:Tissue Engineering; D014162:Transfection
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2