title: Pseudonocardia tetrahydrofuranoxydans sp. nov.
abstract: A Gram-positive, rod-shaped, non-endospore-forming but mycelium-forming actinobacterium (strain K1(T)) was isolated from an enrichment culture containing tetrahydrofuran (THF) as the sole source of carbon. On the basis of its G+C content (71.3 mol%) and of 16S rRNA gene sequence similarity studies, strain K1(T) was shown to belong to the family Pseudonocardiaceae, most closely related to Pseudonocardia hydrocarbonoxydans (99.3 %), P. benzenivorans (98.8 %) and P. sulfidoxydans (98.3 %). The 16S rRNA gene sequence similarity to other Pseudonocardia species was less than 97 %. Chemotaxonomic data [major menaquinone MK-8(H(4)); major fatty acids C(16 : 0) iso, C(15 : 0) iso and C(17 : 1)omega6c] supported the affiliation of strain K1(T) to the genus Pseudonocardia. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain K1(T) from the three species P. benzenivorans, P. sulfidoxydans and P. hydrocarbonoxydans, although all four organisms utilized THF. Strain K1(T) represents a novel species, for which the name Pseudonocardia tetrahydrofuranoxydans sp. nov. is proposed, with the type strain K1(T) (=DSM 44239(T)=CIP 109050(T)).
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Institut für Mikrobiologie, Martin-Luther-Universität Halle, D-06099 Halle, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kohlweyer
authors.affiliation: Institut für Mikrobiologie, Martin-Luther-Universität Halle, D-06099 Halle, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Thiemer
authors.affiliation: Institut für Mikrobiologie, Martin-Luther-Universität Halle, D-06099 Halle, Germany.
authors.forename: Jan R
authors.initials: JR
authors.lastname: Andreesen
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005663:Furans / Q000378:metabolism*; D020459:Genes, rRNA; D005840:Gentian Violet; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010619:Phenazines; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000737:chemistry; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D005663:Furans; C035863:Gram's stain; D010619:Phenazines; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C018674:tetrahydrofuran; D005840:Gentian Violet