title: Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs.
abstract: The migration of B cells into secondary lymphoid organs is required for the generation of an effective immune response. Here we analyzed the involvement of SWAP-70, a Rac-interacting protein involved in actin rearrangement, in B cell entry into lymph nodes. We noted reduced migration of Swap70-/- B cells into lymph nodes in vivo. Swap70-/- B cells rolled and adhered, yet accumulated in lymph node high endothelial venules. This defect was not due to impaired integrin expression or chemotaxis. Instead, Swap70-/- B cells aberrantly regulated integrin-mediated adhesion. During attachment, Swap70-/- B cells showed defective polarization and did not form uropods or stabilize lamellipodia at a defined region. Thus, SWAP-70 selectively regulates processes essential for B cell entry into lymph nodes.
authors.affiliation: Institute of Physiological Chemistry, Dresden University of Technology, 01307 Dresden, Germany.
authors.forename: Glen
authors.initials: G
authors.lastname: Pearce
authors.forename: Veronique
authors.initials: V
authors.lastname: Angeli
authors.forename: Gwendalyn J
authors.initials: GJ
authors.lastname: Randolph
authors.forename: Tobias
authors.initials: T
authors.lastname: Junt
authors.forename: Ulrich
authors.initials: U
authors.lastname: von Andrian
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Schnittler
authors.forename: Rolf
authors.initials: R
authors.lastname: Jessberger
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology; D004268:DNA-Binding Proteins / Q000276:immunology*; D020662:Guanine Nucleotide Exchange Factors / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins / Q000276:immunology*; D016201:Receptors, Lymphocyte Homing / Q000276:immunology*
chemical_list: D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins; D016201:Receptors, Lymphocyte Homing; C113365:Swap70 protein, mouse