title: [Genetic heterogeneity of Borrelia afzelii in the natural focus of the Middle Urals].
abstract: As shown by sequencing the spacer rrf (5S)--rrl (23S) in 72 isolates of B. afzelii (one of the causative agents of Ixodes tick borne Borrelia infections) and the chromosomal gene coding protein P66 in 22 isolates, that in the natural focus located in the Middle Urals two different genetic subgroups (VS461 and NT28) of this genospecies simultaneously circulate. These subgroups are represented by 5 gene variants (rrf) 5S--(rrl) 23S and 5 allelic variants in gene p66. The latter, similarly to spacer gene variants, are not linked with a definite host and occur in different rrf--rrl variants of the infective agent. At the same time the definite species of vectors and carriers may be the host of several different B. afzelii variants, both in the spacer and in the gene coding protein P66, which maintains the genetic heterogeneity of B. afzelii population in the natural focus.
authors.forename: I A
authors.initials: IA
authors.lastname: Fadeeva
authors.forename: E I
authors.initials: EI
authors.lastname: Korenberg
authors.forename: V V
authors.initials: VV
authors.lastname: Nefedova
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Andreĭchuk
authors.forename: A V
authors.initials: AV
authors.lastname: Markov
authors.forename: I A
authors.initials: IA
authors.lastname: Shaginian
mesh_terms: D000483:Alleles; D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics; D015748:Borrelia burgdorferi Group / Q000145:classification / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics; D004197:Disease Reservoirs / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation; D018884:Ixodes / Q000382:microbiology; D008193:Lyme Disease / Q000517:prevention & control*; D008322:Mammals / Q000382:microbiology; D008969:Molecular Sequence Data; D018272:Porins / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012426:Russia / Q000453:epidemiology; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D021901:DNA, Intergenic; C107893:Oms66 protein, Borrelia; D018272:Porins; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S