title: Comparative proteome analysis of laboratory grown Brucella abortus 2308 and Brucella melitensis 16M.
abstract: Brucella species are pathogenic agents that cause brucellosis, a debilitating zoonotic disease that affects a large variety of domesticated animals and humans. Brucella melitensis and Brucella abortus are considered major health threats because of their highly infectious nature and worldwide occurrence. The availability of the annotated genomes for these two species has allowed a comparative proteomics study of laboratory grown B. melitensis 16M and B. abortus 2308 by two-dimensional (2-D) gel electrophoresis and peptide mass fingerprinting. Computer-assisted analysis of the different 2-D gel images of strains 16M and 2308 revealed significant quantitative and qualitative differences in their protein expression patterns. Proteins involved in membrane transport, particularly the high affinity amino acids binding proteins, and those involved in Sec-dependent secretion systems related to type IV and type V secretion systems, were differentially expressed. Differential expression of these proteins may be responsible for conferring specific host preference in the two strains 2308 and 16M.
authors.affiliation: Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510, USA. Michel.Eschenbrenner@Scranton.edu
authors.forename: Michel
authors.initials: M
authors.lastname: Eschenbrenner
authors.forename: Troy A
authors.initials: TA
authors.lastname: Horn
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Wagner
authors.forename: Cesar V
authors.initials: CV
authors.lastname: Mujer
authors.forename: Tabbi L
authors.initials: TL
authors.lastname: Miller-Scandle
authors.forename: Vito G
authors.initials: VG
authors.lastname: DelVecchio
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002003:Brucella abortus / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D017347:Brucella melitensis / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome