Novel dermaseptins from Phyllomedusa hypochondrialis (Amphibia).
Six new antimicrobial peptides structurally related to the dermaseptin family have been isolated from the skin secretion of the amphibian Phyllomedusa hypochondrialis. The primary structures of these molecules named as DShypo 01, 02, 03, 04, 06, and 07 were determined by de novo MS/MS experiments, Edman degradation, and cDNA sequencing. The fifth peptide was found to be precisely the same DS 01 from Phyllomedusa oreades previously described by our group. The majority of the peptides purified from the crude skin secretion could be directly localized and mapped onto a freshly dissected dorsal skin fragment using mass spectrometry-imaging techniques. Comparisons between peptides and commercial drugs on their antibacterial and anti-Leishmania amazonensis efficiencies, associated with peptide lytic effects on mammalian blood cells and surface plasmon resonance interaction studies on immobilized DMPC vesicles, were also performed.
Brand
Guilherme D
GD
Laboratório de Espectrometria de Massa, EMBRAPA-Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
Leite
José Roberto S A
JR
de Sá Mandel
Saulo Martins
SM
Mesquita
Darlan A
DA
Silva
Luciano P
LP
Prates
Maura V
MV
Barbosa
Eder A
EA
Vinecky
Felipe
F
Martins
Graciella R
GR
Galasso
João Henrique
JH
Kuckelhaus
Selma A S
SA
Sampaio
Raimunda N R
RN
Furtado
José R
JR
Andrade
Alan C
AC
Bloch
Carlos
C
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D001001:Anura / Q000378:metabolism*; D006801:Humans; D007891:Leishmania / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013211:Staphylococcus aureus / Q000187:drug effects; D020349:Surface Plasmon Resonance
D029845:Amphibian Proteins; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D010446:Peptide Fragments; C070905:dermaseptin