Transient suppression of PPARgamma directed ES cells into an osteoblastic lineage.
Osteoblasts and adipocytes are believed to share a common progenitor. Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a key role in the switching of these two cell lineages. Here, we demonstrated the differentiation of ES cells into an osteoblastic lineage using siRNA against PPARgamma without the addition of any osteogenic factors. We found that PPARgamma-siRNA downregulated the expression of aP2 mRNA and lipid accumulation, whereas it upregulated the expression of osteocalcin and calcium deposition. These results suggested that ES cells were directed into an osteoblastic lineage. Therefore, transient suppression using PPARgamma-siRNA may be a novel tool to induce differentiation of ES cells into osteoblasts.
Yamashita
Akihiro
A
Research Center for Animal Life Science, Shiga University of Medical Science, Tsukinowa-cho, Seta, Ohtsu 520-2192, Japan.
Takada
Tatsuyuki
T
Nemoto
Ken-ichi
K
Yamamoto
Gaku
G
Torii
Ryuzo
R
D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D019070:Cell Lineage / Q000187:drug effects / Q000502:physiology*; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D053495:Osteopontin; D047495:PPAR gamma / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000235:genetics / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000096:biosynthesis; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D014157:Transcription Factors / Q000096:biosynthesis; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D047495:PPAR gamma; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D014157:Transcription Factors; D053495:Osteopontin; D002118:Calcium