title: Poly(N,N-dimethylacrylamide)-grafted polyacrylamide: a self-coating copolymer for sieving separation of native proteins by CE.
abstract: The potential of a series of newly synthesized poly(N,N-dimethylacrylamide) (PDMA) grafted polyacrylamide (PAM) copolymers (P(AM-PDMA)) as a replaceable separation medium for protein analysis was studied. A comparative study with and without copolymers was performed; the separation efficiency, analysis reproducibility and protein recovery proved that the P(AM-PDMA) copolymers were efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. Furthermore, the size-dependent retardation of native proteins in a representative P(AM-PDMA) copolymer was demonstrated by Ferguson analysis. The results showed that the P(AM-PDMA) copolymers combine the good coating property of PDMA and the sieving property of PAM and could be applied as a sieving matrix for the analysis of native proteins.
authors.affiliation: Groupe de Chimie Analytique de Paris Sud, Faculté de Pharmacie, 5 Rue JB Clement, F-92290 Chatenay-Malabry, France.
authors.forename: Jiyou
authors.initials: J
authors.lastname: Zhang
authors.forename: Nguyet Thuy
authors.initials: NT
authors.lastname: Tran
authors.forename: Jérémie
authors.initials: J
authors.lastname: Weber
authors.forename: Cyrine
authors.initials: C
authors.lastname: Slim
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Viovy
authors.forename: Myriam
authors.initials: M
authors.lastname: Taverna
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D000178:Acrylamides; D011108:Polymers; D011506:Proteins; C429790:poly(N,N-dimethylacrylamide)