[Study on the mustard of polysaccharides].
The objective of this study is to isolate and purify the polysaccharides fractions from mustard and to determine the constituent characters. Hot water extracting and ethanol precipitating method were employed to isolate polysaccharides. After the removal of protein by Sevag method, the purified mustard polysaccharides were dried by frozen drier. The amount of total carbohydrates of mustard polysaccharides was measured with phenol-sulfuric acid method. IR spectrometry, UV-spectrophotometer, and automatic spectropolarimeter were used to determine the characteristic absorption and optical rotation of the polysaccharides, respectively. Size exclusion chromatography with laser light scattering technology was employed to measure the molecular masses. The monosaccharides contained in the mustard polysaccharides were analysed by HPLC. The amount of total carbohydrates in mustard polysaccharides is 98.96%. The molecular weight distribution was 1.42 x 10(4)-2.55 x 10(6), and eighty percent was distributed in 2.1 x 10(5). The monosaccharide constituent of mustard polysaccharides were glucose, fructose, arabinose, galactose and xylose, and their molar proportions are 21.4 : 12.89 : 5.6 : 4.1 : 2.5, respectively.
He
Feng-Ga
FG
College of Chemistry, University of Inner Mongolia Nationalities, Tongliao 028000, China.
Hasenqimeng
D008970:Molecular Weight; D009149:Mustard Plant / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
D010936:Plant Extracts; D011134:Polysaccharides