[Molecular consequences of fixation and tissue processing: the examples of nucleic acids and proteins].
The scientific usefulness of the molecular data obtained from tissue analysis is related directly to the quality of the tissue specimen. The most ideal tissue specimen is the one that carries a complete and unaltered representation of the tissue in vivo. The aim of this review is to provide an overview of the effects of fixation and tissue processing on the content and integrity of nucleic acid and proteins.
Plénat
François
F
Laboratoire d'Histopathologie Expérimentale et Moléculaire, EA4001 Radiopotentialisation: de la pré clinique à la clinique, Université Henri Poincaré Nancy I, Faculté de Médecine, BP 184, 54505 Vandoeuvre les Nancy Cedex.
Montagne
Karine
K
Weinbreck
Nicolas
N
Corby
Sébastien
S
Champigneulle
Jacqueline
J
Antunes
Laurent
L
Bonnet
Corinne
C
Maire
Claudine
C
Monhoven
Nathalie
N
D015925:Cryopreservation; D004247:DNA / Q000032:analysis*; D005404:Fixatives; D005612:Freeze Drying; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008967:Molecular Biology / Q000379:methods; D010336:Pathology / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012313:RNA / Q000032:analysis*
D005404:Fixatives; D011506:Proteins; D012313:RNA; D004247:DNA