[Investigation of discrepant proteins between two breast cancer cell lines with different metastatic abilities].
OBJECTIVE
Metastasis associated proteins play important roles in metastasis. This study was to investigate proteins which may be involved in breast cancer cell metastasis and further explore the potential mechanisms.


METHODS
LM-MCF-7 and MCF-7, two breast cancer cell lines with different metastatic potentials, derived from the same parent cell line, were used in our study. Proteomics and Western blot were applied to identify differentially expressed proteins. Wound healing assay was performed to observe the effect of survivin gene on breast cancer cell migration by transfecting pcDNA3-Sur plasmid into MCF-7 cells.


RESULTS
Eight differently expressed proteins, which were correlated with cell structures, cellular metabolism, apoptosis, protein enfold or interaction, were identified. Protein expression of nm23 and p27 was relatively higher in MCF-7 cells; while the expression of survivin, Bcl-2 and myosin light chain kinase was relatively higher in LM-MCF-7 cells. Increased migration ability was observed in MCF-7 cells which were transfected with pcDNA3-Sur.


CONCLUSIONS
Metastasis associated proteins exist in breast cancer cell lines with different metastatic abilities. Survivin is closely related to the metastasis in breast cancer cells.
Ye
Li-Hong
LH
Key Laboratory of Bioactive Material of Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, P. R. China.
You
Jia-Cong
JC
Guo
Wei
W
Xu
Shao-Feng
SF
Wu
Lian-Ying
LY
Qi
Robert Z
RZ
Zhang
Xiao-Dong
XD
D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D054778:NM23 Nucleoside Diphosphate Kinases / Q000378:metabolism; D009362:Neoplasm Metastasis; D040901:Proteomics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D000077022:Survivin
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D019253:Proto-Oncogene Proteins c-bcl-2; D000077022:Survivin; D050760:Cyclin-Dependent Kinase Inhibitor p27; D009219:Myosin-Light-Chain Kinase; C517938:NME1 protein, human