[Construction and identification of plasmid vector encoding two survivin shRNA].
OBJECTIVE
To construct and identify the eukaryotic expression plasmids encoding two short hairpin RNA (shRNA) of survivin for the purpose of paving the way for the studies of targeted gene therapy for prostatic carcinoma (PCa).


METHODS
Two shRNA of survivin were designed and synthesized respectively, and then both were cloned into plasmids. Finally, the recombinant plasmids were confirmed by sequencing and agarose gel electrophoresis after restriction digestion.


RESULTS
The recombinant plasmids encoding two survivin shRNA were constructed and the aim sequence obtained.


CONCLUSIONS
Successful construction of the recombinant provides a sound basis for the research of targeted gene therapy for PCa.
Yang
Guang-tian
GT
Department of Urology, the Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215004, China.
Shan
Yu-xi
YX
Yang
Dong-rong
DR
D003001:Cloning, Molecular; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D000077022:Survivin; D014162:Transfection
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D000077022:Survivin; D049452:Green Fluorescent Proteins