Antigiardial activity of methanolic extracts from Helianthemum glomeratum Lag. and Rubus coriifolius Focke in suckling mice CD-1.
The antigiardial activity of crude methanolic extracts from Helianthemum glomeratum and Rubus coriifolius, plants used in Mexican traditional medicine for the treatment of diarrhea and dysentery, were demonstrated using experimental infections of Giardia lamblia in suckling female CD-1 mice. In vivo antigiardial activity was studied to determine the dose required to kill 50% of the trophozoites (ED50). Five single-doses between 1.25 and 20 mg extract/kg body weight were tested. Drugs metronidazole and emetine were used as reference. The ED50 (mg/kg) obtained for the extracts and drugs used as reference was 0.125 for Helianthemum glomeratum, 0.506 for Rubus coriifolius, 0.194 for metronidazole and 0.167 for emetine. Both methanolic extracts showed antigiardial activity, the extract of Helianthemum glomeratum was more active than Rubus coriifolius, and its activity is comparable to metronidazole and emetine. Our results hold the perspective for the utilization of the extracts of these plants as an option to develop of novel antigiardial phytodrugs.
Barbosa
Elizabeth
E
Unidad de Investigación Medica en Farmacología de Productos Naturales, Hospital de Pediatría, 2 Piso, Centro Medico Nacional Siglo XXI, IMSS, Av. Cuauhtemoc 330, Col. Doctores, CP 06725 México DF, Mexico.
Calzada
Fernando
F
Campos
Rafael
R
D000818:Animals; D000833:Animals, Suckling; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D030601:Cistaceae / Q000737:chemistry*; D005260:Female; D016829:Giardia lamblia / Q000187:drug effects*; D005873:Giardiasis / Q000469:parasitology / Q000517:prevention & control*; D008519:Medicine, Traditional; D008800:Mexico; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D027824:Rosaceae / Q000737:chemistry*
D000981:Antiprotozoal Agents; D010936:Plant Extracts