Characterization of a brain tumor cell line established from transgenic mice expressing the vasopressin SV-40 T antigen.
We previously reported that transgenic mice produced with a transgene consisting of the SV40 T antigen and vasopressin without the 3'-flanking region exhibit brain tumors and lymphoma. In this study, transgenic mice were produced with the fusion gene containing the SV40 T antigen and the whole vasopressin gene with the 3'-flanking region. Six transgenic mice were generated, five which died after 2-6 weeks. The remaining founder mouse was investigated for fusion gene expression and tumor progression at the age of 6 weeks. Brain tumor cells were characterized for phenotypes and transgene expression. During in vitro cell cultures, the phenotypic appearances at 10, 20, and 30 passages were as a uniform monolayer with similar growth rates. The site of SV40 T antigen integration was in the A2 region of chromosome 11, and SV40 T antigen was expressed at the same level in cells of both earlier and later passages. Thirty passages were probably insufficient to reach crisis and immortalization. These cells enriched brain tumor cell compositions with astrocytes and neuronal cells.
Kim
Sung Hyun
SH
School of Life Science and Biotechnology, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.
Kim
Myoung Ok
MO
Lee
Sang Ryeul
SR
Kim
Kil Soo
KS
Lee
Tae Hoon
TH
Lee
Hoon Taek
HT
Ha
Ji Hong
JH
Kim
Tae Yoon
TY
Ryoo
Zae Young
ZY
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015870:Gene Expression / Q000235:genetics; D007124:Immunoenzyme Techniques; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D019076:Transgenes / Q000235:genetics; D014667:Vasopressins / Q000235:genetics / Q000378:metabolism*
D000952:Antigens, Polyomavirus Transforming; D011993:Recombinant Fusion Proteins; D014667:Vasopressins