title: Efficient intracellular multiplication of Legionella pneumophila in human monocytes requires functional host cell L-type calcium channels.
abstract: The infectious agent of Legionnaires' disease, Legionella pneumophila, multiplies intracellularly in a variety of eukaryotic cells. Genistein, a tyrosine kinase inhibitor, has been shown to block intracellular replication of L. pneumophila without harming the infected host cell. The present study has been performed to investigate the underlying mechanism. We demonstrate that inhibition of intracellular bacterial growth by genistein is not mediated by its protein tyrosine kinase-modulating effect but by inhibition of L-type calcium channels of the infected host cell.
authors.affiliation: Department of Livestock Biotechnology, WZW TU Munich, Freising, Germany. hagen.wieland@wzw.tum.de
authors.forename: Hagen
authors.initials: H
authors.lastname: Wieland
authors.forename: Nicole
authors.initials: N
authors.lastname: Hechtel
authors.forename: Marion
authors.initials: M
authors.lastname: Faigle
authors.forename: Birgid
authors.initials: B
authors.lastname: Neumeister
mesh_terms: D002118:Calcium / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D020847:Estrogen Receptor Modulators / Q000494:pharmacology; D019833:Genistein / Q000378:metabolism; D006801:Humans; D016952:Legionella pneumophila / Q000254:growth & development*; D007877:Legionnaires' Disease / Q000382:microbiology*; D009000:Monocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism
chemical_list: D020746:Calcium Channels, L-Type; D020847:Estrogen Receptor Modulators; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D002118:Calcium