title: Interaction between liposomes and RBC in microvessels in vivo.
abstract: Liposomes are phospholipid vesicles that can serve as carriers of biologically active agents in vitro and in vivo. Here, we describe the movement of liposomes suspended with blood flowing in capillaries. Liposomes were coated with a polymer to extend their lifespan in rat mesenteric blood vessels and detected by fluorescent staining. Liposome activity was observed by intravital microscopy using a high-speed camera system at 5 and 60 min after liposome administration. Liposome velocity was determined using two-dimensional cross-correlation, and blood flow was measured by high-resolution PIV (particle image velocimetry). The results showed that the motion of polymer-coated liposome followed the phase averaged velocity distribution of heartbeats while flowing with red blood cells in microvessels. Liposome particles tend to move toward the near blood vessel wall in the low velocity of blood flow.
authors.affiliation: Department of Quantum Engineering and Systems Science, University of Tokyo, Tokyo, Japan. jeong@utnl.jp
authors.forename: Jae Hong
authors.initials: JH
authors.lastname: Jeong
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Sugii
authors.forename: Motomu
authors.initials: M
authors.lastname: Minamiyama
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Koji
authors.initials: K
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D002196:Capillaries / Q000378:metabolism* / Q000502:physiology; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D018056:Hemorheology / Q000295:instrumentation / Q000379:methods; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000737:chemistry; D011673:Pulsatile Flow / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation / Q000502:physiology
chemical_list: D008081:Liposomes; D011108:Polymers