Studies of monomeric and homodimeric oxazolo[4,5-b]pyridinium cyanine dyes as fluorescent probes for nucleic acids visualization.
The series of recently synthesized monomeric and homodimeric cyanine dyes based on monomethine cyanine chromophore with oxazolo[4,5-b]pyridinium and quinoline end groups [Vassilev A, Deligeorgiev T, Gadjev N, Drexhage K-H. Synthesis of novel monomeric and homodimeric cyanine dyes based on oxazolo[4,5-b]pyridinium and quinolinium end groups for nucleic acid detection, Dyes Pigm 2005;66:135-142] were studied as possible fluorescent probes for nucleic acids detection. Significant fluorescence enhancement and intensity level (quantum yield up to 0.75) was observed for all the dyes in the presence of DNA. The oxazolo[4,5-b]pyridinium cyanines demonstrated high sensitivity as fluorescent stains for post-electrophoretic visualization of nucleic acids in agarose gels upon both VIS and UV transillumination, and the visualized band contained 0.8 ng of dsDNA.
Kovalska
V B
VB
Institute of Molecular Biology and Genetics, National Academy of Science of Ukraine, 150 Zabolotnogo St., 03143 Kyiv, Ukraine.
Tokar
V P
VP
Losytskyy
M Yu
MY
Deligeorgiev
T
T
Vassilev
A
A
Gadjev
N
N
Drexhage
K-H
KH
Yarmoluk
S M
SM
D002232:Carbocyanines / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry; D019281:Dimerization; D004587:Electrophoresis, Agar Gel; D005456:Fluorescent Dyes / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D010080:Oxazoles / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
C478828:BEBO cyanine dye; D002232:Carbocyanines; D005456:Fluorescent Dyes; D007211:Indoles; D009696:Nucleic Acids; D010080:Oxazoles; D011726:Pyridinium Compounds; D004247:DNA