title: Microdialysis for myocardial metabolic surveillance: developing a clinical technique.
abstract: Metabolic surveillance of the myocardium is of great interest in cardiac surgery. Microdialysis allows sampling of chemical substances from the interstitial fluid for immediate analysis. The two objectives of this study were to develop a technique for simple and safe implantation of a commercially available microdialysis probe (CMA-70) into the myocardium and to obtain reference data for further use and metabolic control. Eighteen pigs were used in an experimental ischaemic heart model where the left anterior descending coronary artery was occluded for 20 min. Microdialysis was performed proximally as well as distally to the arterial occlusion site corresponding to a control and an ischaemic area in the heart. Two techniques were tried for probe implantation, using either a pacemaker wire attached to the probe tip or a needle introducer. Metabolic substrates (glucose, lactate, glycerol and pyruvate) were collected before, during and after ischaemia, for up to 6 h. Both techniques were highly effective in registering metabolic changes due to ischaemia with sharp time resolution, but the needle introducer was superior regarding probe durability. It is concluded that the CMA-70 microdialysis probe implanted with the needle introducer allows for an accurate monitoring of myocardial metabolism during a prolonged period of time. Future studies in the human heart are warranted to further validate the technique.
authors.affiliation: Department of Cardiac Surgery, University of Insubria, Varese, Italy. vitmantovani@hotmail.com
authors.forename: Vittorio
authors.initials: V
authors.lastname: Mantovani
authors.forename: Charles
authors.initials: C
authors.lastname: Kennergren
authors.forename: Michel
authors.initials: M
authors.lastname: Goiny
authors.forename: Urban
authors.initials: U
authors.lastname: Ungerstedt
authors.forename: Peter
authors.initials: P
authors.lastname: Lönnroth
authors.forename: Andrea
authors.initials: A
authors.lastname: Sala
authors.forename: Eva
authors.initials: E
authors.lastname: Berglin
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D006801:Humans; D007773:Lactates / Q000097:blood; D008297:Male; D017551:Microdialysis / Q000379:methods*; D017202:Myocardial Ischemia / Q000209:etiology / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism*; D011773:Pyruvates / Q000097:blood; D012015:Reference Standards; D013552:Swine; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007773:Lactates; D011773:Pyruvates; D005990:Glycerol