[Molecular cloning and expression analysis of swamp eel (Monopterus albus) Hprt].
The enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT) plays an important role in the purine salvage pathways. Full-length cDNA of swamp eel (Monopterus albus, the rice field eel) Hprt gene was obtained by the RACE method. The cDNA sequence was 1452 bp in length, which encoded a protein of 218 amino acids. Amino acid identities of Hprt between swamp eel and other vertebrates including human, mouse, chicken and zebrafish were more than 76.7%. Phylogenetic tree analysis based on the amino acid sequences showed that Hprt of swamp eel and zebrafish was in one clade. RT-PCR analysis showed ubiquitous expression pattern of swamp eel Hprt in adult tissues, suggesting that it has a constitutive function and is conserved during evolution.
He
Yan
Y
Center for Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Shang
Xuan
X
Cheng
Han-Hua
HH
Zhou
Rong-Jia
RJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D023701:Smegmamorpha / Q000235:genetics* / Q000378:metabolism; D014714:Vertebrates / Q000145:classification / Q000235:genetics
D029941:Fish Proteins; D007041:Hypoxanthine Phosphoribosyltransferase