Matrix metalloproteinase-2 (MMP-2) regulates astrocyte motility in connection with the actin cytoskeleton and integrins.
Matrix Metalloproteinases (MMPs) play a role in migration of many cell types outside the central nervous system (CNS). Among neural cells, astrocytes are one of the main sources of MMPs in physiological and postlesional conditions. However, no data are available on the possible role of MMPs in astrocyte motility. Using an in vitro model of 2D migration and broad spectrum and selective MMP inhibitors, the authors demonstrated that MMP-2, but not MMP-9, is a key enzyme for astrocyte migration. In support of these data, the authors found constitutive expression of MMP-2 in astrocytes, while MMP-9 was nearly undetectable by gel zymography and immunocytochemical methods. The inhibition of migration by MMP inhibitors correlated with changes in cell morphology and in the organization of the actin cytoskeleton. In parallel, the characteristic focalized distribution of MMP-2 at the migration front observed in control cells became more diffuse and internalized by treatments that inhibited migration. The disruption of actin by cytochalasin D caused the partial recruitment of MMP-2 and gelatinolytic activity into actin aggregates, indicating a connection between the proteinase and the actin cytoskeleton. Finally, the authors found a co-localization of beta1-integrin with MMP-2 at the leading edge of migrating astrocytes. Altogether, these data provide the first evidence for the implication of MMP-2 in astrocyte motility, probably through the interaction of the proteinase with beta1-integrin that could act as a linker between pericellular proteolysis and the actin cytoskeleton.
Ogier
Crystel
C
Neurobiologie des Interactions Cellulaires et Neurophysiopathologie, CNRS UMR 6184. Université de la Méditerranée, Faculté de Médecine de Marseille, IFR Jean Roche, Pierre Dramard 13916, Marseille cedex 20, France.
Bernard
Anne
A
Chollet
Anne-Marie
AM
LE Diguardher
Thierry
T
Hanessian
Stephen
S
Charton
Gérard
G
Khrestchatisky
Michel
M
Rivera
Santiago
S
D000199:Actins / Q000502:physiology*; D000818:Animals; D001253:Astrocytes / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D002448:Cell Adhesion; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D003599:Cytoskeleton / Q000502:physiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D018093:Gelatinases / Q000378:metabolism; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000502:physiology; D016023:Integrins / Q000502:physiology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000502:physiology*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D051379:Mice; D011480:Protease Inhibitors / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012685:Sepharose / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D000199:Actins; D019012:Integrin beta1; D016023:Integrins; D011480:Protease Inhibitors; D012685:Sepharose; D018093:Gelatinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D013936:Thymidine