title: Analysis of RAD51 polymorphism and BRCA1 mutations in Polish women with breast cancer.
abstract: OBJECTIVE
  Breast cancer is one of the major killers worldwide. The objectives of this study were to determine the frequency of BRCA1 germ-line mutations and the RAD51 G/C polymorphism in patients with breast cancer.
  
  
  METHODS
  100 breast cancer women provided blood for mutation analysis. Blood samples age matched healthy individuals (n = 106) served as control. The G/C polymorphism and BRCA1 mutations were determined by PCR-RFLP methods.
  
  
  RESULTS
  The distribution of the genotypes of the G/C polymorphism RAD51 in both control and patients did not differ significantly from those predicted by the Hardy - Weinberg distribution. There were no significant differences in the genotype distributions and allele frequencies between node-positive and node-negative patients. In present study one Ex20insC mutations of BRCA1 gene was identified in women with breast cancer.
  
  
  CONCLUSIONS
  Our study implies that the G/C polymorphism of the RAD51 gene may not be directly involved in the development and=or progression of breast cancer.
authors.affiliation: Laboratory of Molecular Genetics, Department of Pathology, Institute of Polish Mother's Memorial Hospital, Lodz, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Romanowicz-Makowska
authors.forename: B
authors.initials: B
authors.lastname: Smolarz
authors.forename: M
authors.initials: M
authors.lastname: Zadrozny
authors.forename: A
authors.initials: A
authors.lastname: Kulig
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D009154:Mutation; D011044:Poland; D011110:Polymorphism, Genetic; D051135:Rad51 Recombinase / Q000235:genetics*
chemical_list: D019313:BRCA1 Protein; D051135:Rad51 Recombinase