Spectrophotometric determination of trazodone, amineptine and amitriptyline hydrochlorides through ion-pair formation with molybdenum and thiocyanate.
Extraction spectrophotometric method has been developed for the determination of tricyclic drugs such as trazodone (TZH), amineptine (APH) and amitriptyline (ATPH) hydrochlorides in pure form and in the dosage forms coming from different Egyptian markets. The method based on the formation of ion-pairs between these drugs under investigation and inorganic complex of Mo(V)-thiocyanate followed by its extraction with methylene chloride. The optimum conditions for the ion-pairs formation are established. The method permits the determination of TZH, APH and ATPH over the concentration range of 2-28, 2-32 and 1-30 microg ml(-1), respectively. The Sandell sensitivity (S) is found to be 0.105, 0.138 and 0.118 g cm(-2) for TZH, APH and ATPH, respectively. The SD is found to be 0.16-0.377, 0.12-0.259 and 0.091-0.286 and the R.S.D. are 0.14-0.55, 0.12-0.399 and 0.095-0.485 for TZH, APH and ATPH, respectively. The method is applicable for the assay of the investigated drugs in different dosage forms and the results are in good agreement with those obtained by the official method.
Mohamed
Gehad G
GG
Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt. ggenidy@hotmail.com
Nour El-Dien
F A
FA
Khalil
S M
SM
Mohamed
Nehad A
NA
D000042:Absorption / Q000187:drug effects; D000639:Amitriptyline / Q000737:chemistry*; D000929:Antidepressive Agents, Tricyclic / Q000737:chemistry; D001205:Ascorbic Acid / Q000494:pharmacology; D003986:Dibenzocycloheptenes / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006851:Hydrochloric Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D008982:Molybdenum / Q000737:chemistry* / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature; D013861:Thiocyanates / Q000737:chemistry* / Q000494:pharmacology; D013997:Time Factors; D014196:Trazodone / Q000737:chemistry*
D000929:Antidepressive Agents, Tricyclic; D003986:Dibenzocycloheptenes; D012997:Solvents; D013861:Thiocyanates; D000639:Amitriptyline; C011597:amineptin; D008982:Molybdenum; C026976:thiocyanic acid; C031760:thiocyanate; C022175:ammonium molybdate; D001205:Ascorbic Acid; D006851:Hydrochloric Acid; D014196:Trazodone