title: The reliability of the i-STAT clinical portable analyser.
abstract: The purpose of this investigation was to assess the reliability of the i-STAT clinical portable analyser and CG(4)(+) cartridge measures of blood pH, partial pressures of O(2) (pO(2)) and CO(2) (pCO(2)), bicarbonate ([HCO(3)(-)]), base excess (BE), total carbon dioxide (TCO(2)), oxygen saturation (sO(2)) and blood lactate ([BLa(-)]) at various exercise intensities. A comparison between [BLa(-)] measured by the i-STAT and the Accusport lactate analysers during an intermittent treadmill run was also undertaken. The technical error of measurement (TEM%) at rest, at moderate (200W) and maximal exercise (V O(2)max) was acceptable (<15%) for all parameters. The intra-class correlation coefficients for each analyte ranged from weak-to-strong across resting (ICC=0.19-0.96) and moderate (ICC=0.30-0.96) exercise intensities. The ICC for all analytes were observed to be strong following maximal intensity exercise (ICC=0.77-0.95). The comparison of the [BLa(-)] measures between the i-STAT and Accusport showed that the difference between measures was acceptable at both low (<4mmolL(-1)) (-0.39+/-0.27mmolL(-1)), moderate to high concentrations (>4mmolL(-1)) (0.58+/-1.22mmolL(-1)), and across all [BLa(-)] data (0.36+/-1.13mmolL(-1)). In conclusion, the i-STAT clinical analyser and CG(4)(+) cartridge provides reliable measures of a number of blood parameters across exercise intensities. The [BLa(-)] measures from the i-STAT analyser are consistent with that of the Accusport lactate analyser.
authors.affiliation: School of Health and Human Performance, Faculty of Science, Engineering and Health, Central Queensland University, North Rockhampton, Qld, Australia. b.dascombe@cqu.edu.au
authors.forename: B J
authors.initials: BJ
authors.lastname: Dascombe
authors.forename: P R J
authors.initials: PR
authors.lastname: Reaburn
authors.forename: A C
authors.initials: AC
authors.lastname: Sirotic
authors.forename: A J
authors.initials: AJ
authors.lastname: Coutts
mesh_terms: D000328:Adult; D001784:Blood Gas Analysis / Q000295:instrumentation* / Q000592:standards*; D001800:Blood Specimen Collection / Q000379:methods; D016552:Ergometry; D015444:Exercise / Q000502:physiology; D005080:Exercise Test; D005260:Female; D006403:Hematologic Tests / Q000295:instrumentation* / Q000592:standards*; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results
chemical_list: D019344:Lactic Acid