title: In vitro variables of red blood cell components collected by apheresis and frozen 6 and 14 days after collection.
abstract: BACKGROUND
  An automated cell processing system (ACP 215, Haemonetics Corp.) can be used for the glycerolization and deglycerolization of RBC components, but the components must be 6 or fewer days old. Depending on the anticoagulant (CP2D)/additive solution (AS) used, deglycerolized RBCs can be stored at 1 to 6 degrees C for up to 14 days. This study evaluated in vitro variables of apheresis RBC stored for 6 and 14 days at 1 to 6 degrees C before glycerolization and 14 days after deglycerolization.
  
  
  METHODS
  Two units of CP2D/AS-3 leukoreduced RBCs were collected by apheresis from seven donors. One unit was glycerolized and frozen 6 days and the other 14 days after collection. All units were deglycerolized with the ACP 215 and stored at 1 to 6 degrees C for 14 days in AS-3. Several in vitro variables were evaluated during postdeglycerolization storage.
  
  
  RESULTS
  All components had postdeglycerolization RBC recoveries greater than 81 percent and osmolalities of less than 400 mOsm per kg. No significant differences were noted in potassium and supernatant hemoglobin after 14 days of postdeglycerolization storage between RBCs frozen at 6 and 14 days after collection. After 14 days of postdeglycerolization storage, however, the pH, lactate, and ATP levels were slightly lower in RBCs frozen after 14 days.
  
  
  CONCLUSIONS
  The ACP 215 can be used to glycerolize and deglycerolize apheresis RBC components that are up to 14 days of age. It is likely that apheresis components glycerolized at 14 days of age or less can be stored up to 14 days in AS-3 after deglycerolization, but this should be confirmed with in vivo survival studies.
authors.affiliation: Department of Transfusion Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1184, USA.
authors.forename: Heather L
authors.initials: HL
authors.lastname: Grose
authors.forename: Karen M
authors.initials: KM
authors.lastname: Byrne
authors.forename: Jeanne M
authors.initials: JM
authors.lastname: Salata
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Rentas
authors.forename: David F
authors.initials: DF
authors.lastname: Stroncek
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D001781:Blood Component Removal / Q000379:methods / Q000592:standards*; D001793:Blood Preservation / Q000592:standards; D015925:Cryopreservation; D017707:Erythrocyte Transfusion / Q000592:standards*; D004912:Erythrocytes; D005990:Glycerol; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000032:analysis; D009994:Osmolar Concentration; D013997:Time Factors
chemical_list: D007773:Lactates; D000255:Adenosine Triphosphate; D005990:Glycerol