[Comparison of methods to construct a full-length cDNA library].
The use of full-length cDNA libraries is an effective tool to obtain complete gene information in a high-efficiency, high-throughput manner, especially in organisms with huge genomes that are not amenable to whole genome sequencing. In this review, we outlined several methods of full-length cDNA library construction and compared their advantages and disadvantages based on their respective principles. Drawing on our own experience, we described the Cap-trapper method in detail, with an emphasis on its application in wheat full-length cDNA library construction as well as the determination of the ratio of full-length cDNA in a library.
Mao
Xin-Guo
XG
National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Germplasm & Biotechnology/Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China. maoxg2001@sina.com
Jing
Rui-Lian
RL
Kong
Xiu-Ying
XY
Zhao
Guang-Yao
GY
Jia
Ji-Zeng
JZ
D003001:Cloning, Molecular / Q000379:methods*; D015723:Gene Library