title: Bacterial community changes in diesel-oil-contaminated soil microcosms biostimulated with Luria-Bertani medium or bioaugmented with a petroleum-degrading bacterium, Pseudomonas aeruginosa strain WatG.
abstract: The microbial community structure of diesel-oil-contaminated soil microcosms biostimulated with Luria-Bertani medium (LB-BS) or bioaugmented with a petroleum-degrading bacterium, Pseudomonas aeruginosa strain WatG (WatG-BA), was investigated by denaturing gradient gel electrophoresis (DGGE) and by monitoring diesel oil degradation. The degradation in WatG-BA (64.0% +/- 4.2%) was higher than that in LB-BS (49.5% +/- 12.0%) during the first two weeks. The microbial community in WatG-BA, which was markedly dominated by strain WatG, was much simpler than that in LB-BS, where hydrocarbon degraders occurred after a lag of 3-7 days after the addition of diesel oil. The clustering profiles of the DGGE banding patterns of the two soil microcosms were only 12% similar. This difference is probably due to antibacterial substances, such as rhamnolipids, secreted by strain WatG.
authors.affiliation: Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita 10 Nishi 6 Kita-ku, Sapporo 060-0810, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Ueno
authors.forename: Yukiya
authors.initials: Y
authors.lastname: Ito
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Isao
authors.initials: I
authors.lastname: Yumoto
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okuyama
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000378:metabolism; D001407:Bacillus / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001673:Biodegradation, Environmental; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000378:metabolism*; D010578:Petroleum / Q000378:metabolism*; D010802:Phylogeny; D020560:Proteobacteria / Q000235:genetics / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants