Innervation and serotoninergic receptors of the testis interact with local action of interleukin-1beta on steroidogenesis.
Testosterone secretion by Leydig cells is affected by interleukin-1beta (IL-1beta). The aim of the present study was to investigate whether partial denervation of the testis or local administration of a serotonin (5-HT) receptor antagonist could alter the changes in testicular steoidogenesis induced by IL-1beta. Intratesticular administration of IL-1beta was combined with vasectomy or local injection of ketanserin (5-HT type 2 receptor antagonist) in immature hemicastrated rats and the effect of the interventions on testicular steroidogenesis was studied. One day after treatment with local injection of IL-1beta induced a significant rise in testosterone secretion that could be prevented by vasectomy (that also means transection of the inferior spermatic nerve). In a model in which neither IL-1beta nor ketanserin interfered with steroidogenesis, administration of the receptor antagonist just prior to IL-1beta treatment significantly reduced testosterone secretion. Data indicate interaction between testicular nerves and IL-1beta action and interaction between testicular 5-HT2 receptors and local effect of IL-1beta on testosterone secretion.
Gerendai
Ida
I
Neuroendocrine Research Laboratory, Hungarian Academy of Sciences and Semmelweis University, Department of Human Morphology and Developmental Biology, H-1094 Budapest, Tuzoltó u. 58., Hungary. gerendai@ana2.sote.hu
Banczerowski
Péter
P
Csernus
Valér
V
Halász
Béla
B
D000704:Analysis of Variance; D000818:Animals; D004338:Drug Combinations; D007839:Functional Laterality; D053583:Interleukin-1beta / Q000494:pharmacology*; D007650:Ketanserin / Q000494:pharmacology; D008297:Male; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000502:physiology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D013737:Testis / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D014659:Vasectomy / Q000379:methods
D004338:Drug Combinations; D053583:Interleukin-1beta; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D013739:Testosterone; D007650:Ketanserin