title: Expression and function of the gene encoding the voltage-dependent calcium channel beta3-subunit in the mouse placenta.
abstract: Voltage-dependent Ca(2+) channels (VDCC) exist in most excitable cells and their properly regulated activity is essential for critical biological processes as many of these are sensitive to cellular Ca(2+) ion concentration. The ancillary cytoplasmic Ca(2+) channel beta subunits (CACNB) modulate Ca(2+) channel function and are required to enhance the number of functional channels in the plasma membrane. There are four genes encoding CACNB subunits and the gene encoding CACNB3 is over expressed in hyperplastic placentas of mouse interspecies hybrids. To determine the role of CACNB3 in the mouse placenta, we performed an expression and function analysis. Our results show that Cacnb3 exhibits specific spatial and temporal expression in the mouse placenta. Deletion of Cacnb3 does not produce a strong placental phenotype, which may be due to expression of other CACNB subunit encoding genes; however, sporadic occurrence of a labyrinthine architecture phenotype, characterized by reduced density of fetal blood vessels and decrease in pericyte number, could be observed. Down-regulation of Cacnb3 expression did not rescue placental hyperplasia in a model of interspecies hybrid placentas, which indicates that up-regulation in the hyperplastic placentas is a downstream event.
authors.affiliation: Department of Development and Genetics, Evolutionary Biology Center, Uppsala University, Norbyvägen 18A, SE-75236 Uppsala, Sweden.
authors.forename: U
authors.initials: U
authors.lastname: Singh
authors.forename: T
authors.initials: T
authors.lastname: Sun
authors.forename: C
authors.initials: C
authors.lastname: Looman
authors.forename: R
authors.initials: R
authors.lastname: Heuchel
authors.forename: R
authors.initials: R
authors.lastname: Elliott
authors.forename: M
authors.initials: M
authors.lastname: Freichel
authors.forename: M
authors.initials: M
authors.lastname: Meissner
authors.forename: V
authors.initials: V
authors.lastname: Flockerzi
authors.forename: R
authors.initials: R
authors.lastname: Fundele
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D005260:Female; D017353:Gene Deletion; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D010920:Placenta / Q000473:pathology / Q000502:physiology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: C510225:Cacnb3 protein, mouse; D015220:Calcium Channels; D017931:DNA Primers; D012333:RNA, Messenger; D004247:DNA