title: A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes.
abstract: During infection, adenovirus-associated virus (AAV) undergoes microtubule-dependent retrograde transport as part of a program of vectorial transport of viral genome to the nucleus. A microtubule binding assay was used to evaluate the hypothesis that cytoplasmic dynein mediates AAV interaction with microtubules. Binding of AAV serotype 2 (AAV2) was enhanced in a nucleotide-dependent manner by the presence of total cellular microtubule-associated proteins (MAPs) but not cytoplasmic dynein-depleted MAPs. Excess AAV2 capsid protein prevented microtubule binding by AAV serotypes 2, 5, and rh.10, as well as adenovirus serotype 5, indicating that similar binding sites are used by these viruses.
authors.affiliation: Department of Genetic Medicine, Weill Medical College of Cornell University, 515 E. 71st Street, S-1000, New York, NY 10021, USA. geneticmedicine@med.cornell.edu
authors.forename: Samir
authors.initials: S
authors.lastname: Kelkar
authors.forename: Bishnu P
authors.initials: BP
authors.lastname: De
authors.forename: Guangping
authors.initials: G
authors.lastname: Gao
authors.forename: James M
authors.initials: JM
authors.lastname: Wilson
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Crystal
authors.forename: Philip L
authors.initials: PL
authors.lastname: Leopold
mesh_terms: D000256:Adenoviridae / Q000472:pathogenicity / Q000502:physiology; D000257:Adenoviridae Infections / Q000378:metabolism / Q000821:virology; D001665:Binding Sites; D001692:Biological Transport; D036022:Capsid Proteins / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism*; D000229:Dependovirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D004398:Dyneins / Q000378:metabolism*; D016679:Genome, Viral; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism / Q000821:virology; D010322:Parvoviridae Infections / Q000378:metabolism / Q000821:virology; D012703:Serotyping
chemical_list: D036022:Capsid Proteins; D008869:Microtubule-Associated Proteins; D004398:Dyneins