Proliferation of neural precursors in the subventricular zone after chemical lesions of the nigrostriatal pathway in rat brain.
The proliferative activity of neural precursors from the subventricular zone (SVZ) was investigated after a unilateral lesion was formed in the nigrostriatal pathway in adult rats. The lesion was formed by unilateral injection of 6-hydroxydopamine (6-OHDA) into the nigrostriatal pathway, and then bromodeoxyuridine (BrdU) was injected (ip) for 4 days or 2 weeks 10 days after the lesion was formed. The rats were killed, and the brain sections were immunohistochemically stained to detect the expression of BrdU, polysialylated neural-cell-adhesion molecule (PSA-NCAM), glial fibrillary acidic protein (GFAP) and tyrosine hydroxylase (TH) in the SVZ and the striatum (STR). The results showed that the BrdU(+) cells increased significantly in the SVZ, ipsilateral to the lesion at 2 weeks after the lesion. The PSA-NCAM(+) and GFAP(+) cells were also increased in the SVZ at this time. Some BrdU-labeled cells were seen in the same side of the STR and were double-labeled with PSA-NCAM. These cells had a tendency to migrate from the SVZ to the STR. The number of positive cells decreased at 4 weeks after the lesion was formed. The number of nigrostriatal projections with TH(+) decreased significantly in the STR on the lesion side, and the level of decrease was related to the quantity of BrdU-labeled cells at 2 weeks. These results indicate that the neural precursors in the SVZ of adult rats may increase after a lesion has been formed in the nigrostriatal pathway, and these cells might migrate into the STR on the same side.
Liu
Bing Fang
BF
Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
Gao
Er Jing
EJ
Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
Zeng
Xian Zhi
XZ
Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
Ji
Man
M
Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
Cai
Qing
Q
Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
Lu
Qiang
Q
Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
Yang
Hui
H
Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
Xu
Qun Yuan
QY
Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China. Electronic address: xuqy@cpums.edu.cn.
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001973:Bromodeoxyuridine; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D003714:Denervation; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008297:Male; D009410:Nerve Degeneration / Q000139:chemically induced / Q000503:physiopathology; D009416:Nerve Regeneration / Q000502:physiology*; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D009434:Neural Pathways / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D009473:Neuronal Plasticity / Q000502:physiology*; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D012794:Sialic Acids / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013378:Substantia Nigra / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D039842:Neural Cell Adhesion Molecule L1; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D001973:Bromodeoxyuridine