title: Macrophage-stimulating activity of polysaccharides extracted from fruiting bodies of Coriolus versicolor (Turkey Tail Mushroom).
abstract: The macrophage-stimulating effect of polysaccharides extracted from Coriolus versicolor (Turkey Tail mushroom) was investigated, and their effectiveness was compared with that of lipopolysaccharide (LPS). The purified polysaccharide (CV-S2-Fr.I) of C. versicolor obtained by Sepharose CL-6B gel chromatography stimulated macrophage lysosomal enzyme activity by 250% at a concentration of 100 microg/mL, which was higher than that of LPS at the same concentration. When CV-S2-Fr.I was used in combination with interferon-gamma, there was a marked cooperative induction of nitric oxide production. However, CV-S2-Fr.I had no effect on nitric oxide production by itself. The proportion of C3-positive macrophages in the CV-S2-Fr.I group increased by 7.2-fold compared with the control group.
authors.affiliation: Department of Biotechnology, Daegu University, Gyungbuk, Korea.
authors.forename: Sang-Chul
authors.initials: SC
authors.lastname: Jeong
authors.forename: Byung-Keun
authors.initials: BK
authors.lastname: Yang
authors.forename: Guk-Nam
authors.initials: GN
authors.lastname: Kim
authors.forename: Hun
authors.initials: H
authors.lastname: Jeong
authors.forename: Michael A
authors.initials: MA
authors.lastname: Wilson
authors.forename: Yip
authors.initials: Y
authors.lastname: Cho
authors.forename: K Sundar
authors.initials: KS
authors.lastname: Rao
authors.forename: Chi-Hyun
authors.initials: CH
authors.lastname: Song
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D002478:Cells, Cultured; D004347:Drug Interactions; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000032:analysis
chemical_list: D008070:Lipopolysaccharides; D009569:Nitric Oxide; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II