Electrical probing of endothelial cell behaviour on a fibronectin/polystyrene/thiol/gold electrode by Faradaic electrochemical impedance spectroscopy (EIS).
The electrochemical impedance spectroscopy (EIS) technique has been shown to be an effective tool for monitoring endothelial cell behaviour on a multilayer functionalised gold electrode. Polystyrene, a reproducible model substrate, is deposited as a thin layer on a thiol functionalised gold electrode. Fibronectin, a protein promoting endothelial cell adhesion, is then adsorbed on the polystyrene surface. The different steps of this multilayer assembly are characterized by Faradaic impedance. The charge transfer resistance and the capacitance for the total layer are modified at each step according to the electrical properties of each layer. This gives the endothelial cells' electrical state in terms of its resistive and capacitive properties. In this study, the endothelial cell layer presents a specific charge transfer resistance equal to 1.55 kOmega cm(2) with no large defects in the cell layer, and a specific capacitance equal to few microF cm(-2) explained by the existence of pseudopods. These electrical properties are correlated to the endothelial cell viability, adhesion and cytoskeleton organization.
Bouafsoun
Amira
A
CEGELY, UMR-CNRS 5005, Ecole Centrale de Lyon, 69134 Ecully Cedex, France.
Helali
Saloua
S
Mebarek
Saida
S
Zeiller
Caroline
C
Prigent
Annie-France
AF
Othmane
Ali
A
Kerkeni
Abdelhamid
A
Jaffrézic-Renault
Nicole
N
Ponsonnet
Laurence
L
D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D005353:Fibronectins / Q000737:chemistry* / Q000494:pharmacology*; D006046:Gold / Q000737:chemistry; D008839:Microelectrodes; D011137:Polystyrenes / Q000737:chemistry; D013057:Spectrum Analysis / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D011137:Polystyrenes; D013438:Sulfhydryl Compounds; D006046:Gold