title: Light-dependent subcellular localization of nucleoside diphosphate kinase-1 in Neurospora crassa.
abstract: Nucleoside diphosphate kinase (NDK) is a housekeeping enzyme localized in cellular organelles and distributed in various organs in prokaryotes and eukaryotes. In Neurospora crassa, NDK-1 is suggested to control catalases in response to heat, oxidative stress and light. In this study, we identified the presence of NDK-1 during most developmental stages in submerged mycelia, aerial hyphae, asexual conidia and perithecia, and the localization of it in soluble, mitochondrial, nuclear and membrane fractions in the mycelial cell. A light-dependent localization of NDK-1 was shown by Western blotting and immunohistochemical analysis using anti-NDK-1 antibody. In the mycelia, NDK-1 was compartmentalized on the plasma membrane in darkness, while it was relocated in the cytoplasm under light. These results suggest that NDK-1 protein was translocated from the plasma membrane to cytoplasm in response to light, and may interact with catalase.
authors.affiliation: Kihara Institute for Biological Research, Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Kohji
authors.initials: K
authors.lastname: Hasunuma
mesh_terms: D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology; D002467:Cell Nucleus / Q000201:enzymology; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D007150:Immunohistochemistry; D008027:Light; D008928:Mitochondria / Q000201:enzymology; D025282:Mycelium / Q000166:cytology / Q000201:enzymology*; D009492:Neurospora crassa / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D009701:Nucleoside-Diphosphate Kinase / Q000032:analysis* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D009701:Nucleoside-Diphosphate Kinase