title: Functional platelet-activating factor receptors in immature forms of leukemic blasts.
abstract: Platelet-activating factor (PAF) is a phospholipid mediator with potent immunoregulatory activities on mature leukocytes. PAF modulates leukocyte cytosolic Ca2+ concentration ([Ca2+]i) through a Gq mediated pathway. We highlight, for the first time, Gq transcripts, PAF receptor (PAF-R) transcripts and protein in blast cells of acute myeloid (AML) and lymphoid (ALL) leukemia patients. PAF stimulated [Ca2+]i in leukemic blast cells; PAF effects being prevented by a specific PAF-R antagonist. In conclusion, functional PAF-R are present in blast cells of patients with acute leukemia; a result that could be of physiologic importance regarding the important effect of PAF on leukocytes maturation and functions.
authors.affiliation: UMR CNRS 6101, Centre National de la Recherche Scientifique, Université de Limoges, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Reynaud
authors.forename: Emilie
authors.initials: E
authors.lastname: Malissein
authors.forename: Magali
authors.initials: M
authors.lastname: Donnard
authors.forename: Dominique
authors.initials: D
authors.lastname: Bordessoule
authors.forename: Pascal
authors.initials: P
authors.lastname: Turlure
authors.forename: Franck
authors.initials: F
authors.lastname: Trimoreau
authors.forename: Yves
authors.initials: Y
authors.lastname: Denizot
mesh_terms: D000208:Acute Disease; D001752:Blast Crisis; D002118:Calcium / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D007951:Leukemia, Myeloid / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D010980:Platelet Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic
chemical_list: D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D002118:Calcium