Conditional deletion of the colony stimulating factor-1 receptor (c-fms proto-oncogene) in mice.
Colony stimulating factor-1 (CSF-1) is the primary regulator of the mononuclear phagocytic lineage acting through its transmembrane tyrosine kinase receptor, CSF-1R, that is the product of the c-fms proto-oncogene. Null mutations in either the ligand or the receptor genes result in a severe osteopetrosis as well as a number of other phenotypes, including reproductive defects and perturbations in organ development. The CSF-1R is also expressed in oocytes, myoblast progenitors, decidual, and trophoblastic cells. To distinguish cell type specific phenotypes, we have created a conditional allele of the Csf1r by placing LoxP sites around Exon 5 of the Csf1r gene in mice. Excision of this floxed sequence results in a null allele that in the homozygous state gives a phenotype indistinguishable of the complete Csf1r null mutant mouse. This conditional allele will prove extremely valuable to study the spatial and temporal roles of CSF-1R.
Li
Jia
J
Department of Development and Molecular Biology, Center for the Study of Reproductive Biology and Women's Health, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Chen
Ken
K
Zhu
Liyin
L
Pollard
Jeffrey W
JW
D000818:Animals; D005260:Female; D018390:Gene Targeting; D016236:Genes, fms / Q000235:genetics*; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010641:Phenotype; D016184:Receptors, Colony-Stimulating Factor / Q000235:genetics*; D017384:Sequence Deletion
D016184:Receptors, Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor