Derivation and characterization of hepatocytes from embryonic stem cells in vitro.
From a therapeutic perspective on liver, the use of embryonic stem (ES) technology in the generation of a large number of high-functional hepatocytes developed from ES cells for cell transplantation is anticipated. We have explored a three-dimensional culture system in which hepatocytes differentiated from mouse ES cells by transfection with the hepatocyte nuclear factor-3beta possess high metabolic functions that can be maintained long term.
Ishizaka
Shigeaki
S
Program in Tissue Engineering, Department of Parasitology, Nara Medical University, Japan.
Ouji
Yukiteru
Y
Yoshikawa
Masahide
M
Nakatani
Kazuki
K
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology*; D051379:Mice; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D015415:Biomarkers