title: Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus.
abstract: The NMR structure of a 12-mer RNA derived from the helix 6 of SRP RNA from Pyrococcus furiosus, whose loop-closing base pair is U.G, was determined, and the structural and thermodynamic properties of the RNA were compared with those of a mutant RNA with the C:G closing base pair. Although the structures of the two RNAs are similar to each other and adopt the GNRR motif the conformational stabilities are significantly different to each other It was suggested that weaker stacking interaction of the GAAG loop with the U:G closing base pair in 12-mer RNA causes the lower conformational stability.
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, Chiba, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okada
authors.forename: Mari
authors.initials: M
authors.lastname: Takahashi
authors.forename: Taiichi
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Gota
authors.initials: G
authors.lastname: Kawai
authors.forename: Kouji
authors.initials: K
authors.lastname: Nakamura
authors.forename: Akio
authors.initials: A
authors.lastname: Kanai
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D020027:Pyrococcus furiosus / Q000235:genetics*; D020871:RNA Stability; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D018271:Signal Recognition Particle / Q000235:genetics*; D013816:Thermodynamics
chemical_list: D009841:Oligonucleotides; D012329:RNA, Bacterial; D018271:Signal Recognition Particle