Rationalizing membrane protein overexpression.
Functional and structural studies of membrane proteins usually require overexpression of the proteins in question. Often, however, the 'trial and error' approaches that are mainly used to produce membrane proteins are not successful. Our rapidly increasing understanding of membrane protein insertion, folding and degradation means that membrane protein overexpression can be more rationalized, both at the level of the overexpression host and the overexpressed membrane protein. This change of mindset is likely to have a significant impact on membrane protein research.
Wagner
Samuel
S
Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.
Bader
Mirjam Lerch
ML
Drew
David
D
de Gier
Jan-Willem
JW
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis*
D001426:Bacterial Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins