title: [Molecular consequences of fixation and tissue processing: the examples of nucleic acids and proteins].
abstract: The scientific usefulness of the molecular data obtained from tissue analysis is related directly to the quality of the tissue specimen. The most ideal tissue specimen is the one that carries a complete and unaltered representation of the tissue in vivo. The aim of this review is to provide an overview of the effects of fixation and tissue processing on the content and integrity of nucleic acid and proteins.
authors.affiliation: Laboratoire d'Histopathologie Expérimentale et Moléculaire, EA4001 Radiopotentialisation: de la pré clinique à la clinique, Université Henri Poincaré Nancy I, Faculté de Médecine, BP 184, 54505 Vandoeuvre les Nancy Cedex.
authors.forename: François
authors.initials: F
authors.lastname: Plénat
authors.forename: Karine
authors.initials: K
authors.lastname: Montagne
authors.forename: Nicolas
authors.initials: N
authors.lastname: Weinbreck
authors.forename: Sébastien
authors.initials: S
authors.lastname: Corby
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Champigneulle
authors.forename: Laurent
authors.initials: L
authors.lastname: Antunes
authors.forename: Corinne
authors.initials: C
authors.lastname: Bonnet
authors.forename: Claudine
authors.initials: C
authors.lastname: Maire
authors.forename: Nathalie
authors.initials: N
authors.lastname: Monhoven
mesh_terms: D015925:Cryopreservation; D004247:DNA / Q000032:analysis*; D005404:Fixatives; D005612:Freeze Drying; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008967:Molecular Biology / Q000379:methods; D010336:Pathology / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012313:RNA / Q000032:analysis*
chemical_list: D005404:Fixatives; D011506:Proteins; D012313:RNA; D004247:DNA