Effect of daily parathyroid hormone (1-34) on lumbar fusion in a rat model.
BACKGROUND
Despite numerous studies evaluating the anabolic effects of intermittent administration of parathyroid hormone (PTH) on bone, there are no published studies examining its effect on spinal fusion outcomes.


OBJECTIVE
To determine the effect of daily injection of human recombinant PTH(1-34) on posterolateral lumbar fusions in a rat model.


METHODS
Prospective, case-controlled, preclinical animal study.


METHODS
Manual palpation and serum osteocalcin.


METHODS
Single-level, intertransverse process spinal fusions were performed with iliac crest autograft in 56 Sprague-Dawley rats. Animals received daily injections of placebo or PTH(1-34). At 6 weeks, fusion masses were assessed by manual palpation. Serum osteocalcin levels were assessed in a subset of the animals.


RESULTS
Manual palpation revealed the control group to have a fusion rate of 37% (10/27) and the PTH(1-34)-treated group to have a fusion rate of 52% (15/29). Mean serum osteocalcin levels were 59.8 and 88.6 ng/L for the control and PTH(1-34) groups, respectively.


CONCLUSIONS
There was a trend towards greater fusion rate in the PTH(1-34) group as compared with the placebo group. Further, PTH(1-34) administration was associated with a significant increase in osteocalcin levels. Certainly, further investigations are warranted, as an injectable agent capable of increasing fusion rates would be of great clinical value.
Lawrence
James P
JP
Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, CT 06520-8071, USA.
Ennis
Frank
F
White
Andrew P
AP
Magit
David
D
Polzhofer
Gert
G
Drespe
Inneke
I
Troiano
Nancy W
NW
Grauer
Jonathan N
JN
D000818:Animals; D005260:Female; D006801:Humans; D023421:Models, Animal; D015675:Osteocalcin / Q000097:blood; D010173:Palpation; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D013123:Spinal Fusion
D010281:Parathyroid Hormone; D010446:Peptide Fragments; D011994:Recombinant Proteins; D015675:Osteocalcin