title: Cytokine and C-reactive protein profiles induced by porcine circovirus type 2 experimental infection in 3-week-old piglets.
abstract: The purpose of this study was to determine serum profiles of cytokines at a protein level and Creactive protein (CRP) during the development of postweaning multisystemic wasting syndrome (PMWS) in experimentally inoculated pigs. Levels of serum IFN-alpha, IL-6, IL-10, and CRP were examined for a 35-day period in 10 piglets experimentally infected with PCV2 at 3 weeks of age. Four of the infected piglets developed severe PMWS at 14 to 21 days post-infection (d.p.i.) and died prior to termination of the experiment. The remaining six PCV2-infected piglets experienced transient fever, but did not display overt clinical signs of PMWS and were considered as subclinically infected. A bioassay was used to detect IL-6 and ELISAs were used to detect IFN-alpha, IL-10, and CRP. There were no significant differences in cytokine or CRP expression from 0 to 7 d.p.i. between the PMWS-affected and the subclinically infected piglets. Levels of IL-10 and CRP were elevated from 10 and 14 d.p.i. respectively in the PMWS-affected piglets compared to the subclinically infected piglets. There were no significant differences in IFN-alpha and IL-6 expression between the PMWS-affected piglets and the subclinically infected piglets. The present study shows that elevated levels of serum CRP and IL-10 were associated with PCV2-infected piglets that subsequently developed severe PMWS. This may help to provide further insight into the immunoaetiogenesis of this syndrome.
authors.affiliation: Department of Veterinary Science, Queen's University Belfast, Stoney Road, Stormont, Belfast, United Kingdom. s.stevenson@qub.ac.uk
authors.forename: Leanne S
authors.initials: LS
authors.lastname: Stevenson
authors.forename: Ken
authors.initials: K
authors.lastname: McCullough
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vincent
authors.forename: Deirdre F
authors.initials: DF
authors.lastname: Gilpin
authors.forename: Artur
authors.initials: A
authors.lastname: Summerfield
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
authors.forename: Francis
authors.initials: F
authors.lastname: McNeilly
authors.forename: Brian M
authors.initials: BM
authors.lastname: Adair
authors.forename: Gordon M
authors.initials: GM
authors.lastname: Allan
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000821:virology*; D002097:C-Reactive Protein / Q000378:metabolism*; D018173:Circoviridae Infections / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary / Q000821:virology; D017925:Circovirus / Q000276:immunology / Q000472:pathogenicity*; D016207:Cytokines / Q000097:blood*; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D019282:Wasting Syndrome / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D014886:Weaning
chemical_list: D016207:Cytokines; D002097:C-Reactive Protein