HTS technologies in biopharmaceutical discovery.
The concepts and philosophies of HTS can be productively applied to the discovery of new biopharmaceuticals. It is now possible, comprehensively and systematically, to enumerate, clone, produce and screen all secreted proteins, by building upon knowledge accumulated over the past two decades in HTS, genomics and parallel protein expression technologies. Each of the crucial operational components (comprehensive and high-quality cDNA library construction, proper protein-sequence classification, high-throughput protein production, medically relevant assays, state-of-the-art screening and data management) must be optimized to increase the chances of success. In this review, we draw comparisons between small-molecule and protein screening to illuminate common underlying principles as well as differences between the two operations.
Wu
Ge
G
Five Prime Therapeutics, 1650 Owens St., Suite 200, San Francisco, CA 94158, USA. ge.wu@FivePrime.com
Doberstein
Stephen K
SK
D000818:Animals; D001702:Biopharmaceutics / Q000379:methods*; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004345:Drug Industry / Q000379:methods; D006801:Humans; D013678:Technology, Pharmaceutical / Q000379:methods*