CACTA and MITE transposon distributions on a genetic map of rice using F15 RILs derived from Milyang 23 and Gihobyeo hybrids.
Up to 35% of the rice genome consists of various kinds of transposons, and CACTA and MITE are two of the major class 2 DNA transposons in the genome. We have employed the consensus sequences of Rim2/Hipa CACTA, Stowaway MITE Pangrangja, and Tourist MITE Ditto for transposon display (TD) analysis to locate them on a genetic map, with 58 SSR markers used to anchor them. The TD analysis produced a high profile of the polymorphisms between the parental lines, Oryza sativa var. Gihobyeo/O. sativa var. Milyang, in intraspecific F15 RIL lines, locating 368 markers of Rim2/Hipa CACTA, 78 markers of Tourist MITE Ditto, and 22 markers of Stowaway MITE Pangrangja. In the segregation analysis, non-parental segregating bands and segregation distortion bands were observed. The recombinant genetic map spans 3023.9 cM, with 5.7 cM the average distance between markers. The TD markers were distributed unequally on the chromosomes because many TD markers were located in pericentric chromosomal regions except in the cases of chromosomes 2, 3, 6 and 9. Although the number of transposon markers was not sufficient to include all rice class 2 transposons, the current map of CACTA and MITE transposons should provide new insight into the genome organization of rice since no previous DNA transposon map is available.
Kwon
Soon-Jae
SJ
Division of Biotechnology, Kangwon National University, Chunchon 200-701, Korea.
Hong
Sung-Won
SW
Son
Jae-Han
JH
Lee
Ju Kyong
JK
Cha
Yong-Soon
YS
Eun
Moo-Young
MY
Kim
Nam-Soo
NS
D002874:Chromosome Mapping; D020090:Chromosome Segregation; D032461:Chromosomes, Plant / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D018744:DNA, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D006824:Hybridization, Genetic; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*
D004251:DNA Transposable Elements; D018744:DNA, Plant; D005819:Genetic Markers; D010940:Plant Proteins