Detection of gentoxicity of benzidine and its derivatives with the Escherichia coli DJ 702 lacZ reversion mutagenicity assay.
OBJECTIVE
The feasibility of Escherichia coli DJ 702 lacZ mutagenicity assay to detect genotoxicity of benzidine and its derivatives was evaluated.


RESULTS
DJ 702 strain was grown overnight at 30 degrees C in Luria-Bertani (LB) medium containing some components, such as chloramphenicol, ampicillin, delta-aminolevulinic acid, isopropyl-beta-d-thiogalactoside, and trace element mix. The mixtures of a bacterial culture and tested chemical at indicated doses were incubated at 30 degrees C for 30 min. Subsequently, 2 ml of molten top agar was added and the resulting mixtures were immediately poured onto a minimal lactose (ML) plate. Plates were incubated at 30 degrees C for 48 h. The number of colonies was determined by visual scoring. In this study, results showed that all the tested chemicals were mutagenic to DJ 702 strain.


CONCLUSIONS
E. coli lac mutagenicity assay using DJ 702 strain can detect the mutagenicity of benzidine and its derivatives.


CONCLUSIONS
We detected the mutagenicity of benzidine and its derivatives in E. coli lac mutagenicity assay using DJ 702, indicating that this assay may be used to detect benzidine and its derivatives in a powerful, sensitive, and convenient mutagenesis assay.
Chen
S C
SC
Department of Biotechnology, National Kaohsiung Normal University, Taiwan. osycchna@ksts.seed.net.tw
Lin
C S
CS
Liang
S H
SH
Chuang
J Y
JY
D001560:Benzidines / Q000737:chemistry / Q000633:toxicity*; D019388:Cytochrome P-450 CYP1A2 / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D006801:Humans; D007763:Lac Operon / Q000235:genetics*; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000235:genetics
D001560:Benzidines; D004269:DNA, Bacterial; D009153:Mutagens; C029876:benzidine; D019388:Cytochrome P-450 CYP1A2