Modelling chronic exposure to contaminated soil: a toxicokinetic approach with the terrestrial snail Helix aspersa.
To enlarge the possibilities of using organisms of the soil fauna to assess the bioaccumulative potential of chemicals, the kinetic of soil cadmium (Cd) transfer to the terrestrial gastropod Helix aspersa was investigated under laboratory conditions during a long-term experiment (6 months). During the exposure phase (3 months), juvenile snails were subjected to three different concentrations of Cd spiked in artificial ISO soil (ISO 0, 20 and 100 mg Cd kg(-1)) and to a field soil (ME4) industrially contaminated by 20 mg Cd kg (-1). For both soils, internal steady-state Cd concentrations were reached in the viscera of the snails, the main storage organ for Cd, after 2 weeks of exposure whatever the Cd concentration in soil. The equilibrium concentrations in the viscera were 0.7 (+/-0.1), 11.3 (+/-2.4), 73.3 (+/-4.8) and 6.3 (+/-1.3) mg Cd kg(-1) dry mass for ISO 0, ISO 20, ISO 100 and ME4, respectively. During the depuration phase (3 months), from 0 to 52% of the accumulated Cd in the viscera were removed by excretion or relocation in the foot. However, the snails were not able to depurate down to initial concentrations. Data were modelled by integrating a specific growth rate constant into one-compartment toxicokinetic models. This allowed the calculation of Cd uptake rates that can be used as indicators of metal bioavailability. Since this parameter was found to be lower for snails exposed to the field soil ME4, we concluded that lower Cd bioavailability in this field soil was responsible of the lower transfer to the snails compared to the ISO 20 soil, even though they were polluted to similar extents. Internal validation showed that the toxicokinetic models could be applied for predictive purposes, promising for the development of a bioaccumulation directive for terrestrial environment.
Gimbert
Frédéric
F
University of Franche-Comté, Department of Environmental Biology, EA 3184 MR UsC INRA, Besançon, France.
de Vaufleury
Annette
A
Douay
Francis
F
Scheifler
Renaud
R
Coeurdassier
Michaël
M
Badot
Pierre-Marie
PM
D000818:Animals; D001682:Biological Availability; D002104:Cadmium / Q000493:pharmacokinetics*; D004784:Environmental Monitoring; D006372:Helix, Snails / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D012989:Soil Pollutants / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D018675:Toxicity Tests
D012989:Soil Pollutants; D002104:Cadmium