Thermaerobacter litoralis sp. nov., a strictly aerobic and thermophilic bacterium isolated from a coastal hydrothermal field.
A novel thermophilic bacterium, strain KW1(T), was isolated from a coastal hydrothermal field on the Satsuma Peninsula, Kagoshima Prefecture, Japan. The variably Gram-stained cells were motile rods with flagella, did not form spores and proliferated at 52-78 degrees C (optimum, 70 degrees C), pH 5-8 (optimum, pH 7) and 0-4.5 % NaCl (optimum, 1.0 %). The novel isolate was a strictly aerobic heterotroph that utilized complex proteinaceous substrates as well as a variety of carboxylic acids and amino acids. The G+C content of the genomic DNA was 70.8 mol%. Analysis of 16S rRNA gene sequences indicated that strain KW1(T) is closely related to Thermaerobacter subterraneus C21(T) (98.4 % sequence similarity). However, the DNA-DNA hybridization value for strain KW1(T) and T. subterraneus ATCC BAA-137(T) was below 46 %. On the basis of the molecular and physiological traits of strain KW1(T), it represents a novel species of the genus Thermaerobacter, for which the name Thermaerobacter litoralis sp. nov. is proposed. The type strain is KW1(T) (=JCM 13210(T)=DSM 17372(T)).
Tanaka
Reiji
R
Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake, Kyoto 606-8502, Japan.
Kawaichi
Satoshi
S
Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake, Kyoto 606-8502, Japan.
Nishimura
Hiroshi
H
Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake, Kyoto 606-8502, Japan.
Sako
Yoshihiko
Y
Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake, Kyoto 606-8502, Japan.
D000332:Aerobiosis; D000596:Amino Acids / Q000378:metabolism; D001482:Base Composition; D002264:Carboxylic Acids / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D005840:Gentian Violet; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D006131:Growth Inhibitors / Q000494:pharmacology; D045482:Hot Springs / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007564:Japan; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010619:Phenazines; D010802:Phylogeny; D011506:Proteins / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013171:Spores, Bacterial; D013696:Temperature; D014871:Water Microbiology
D000596:Amino Acids; D002264:Carboxylic Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C035863:Gram's stain; D006131:Growth Inhibitors; D010619:Phenazines; D011506:Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D005840:Gentian Violet