Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method.
Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2.
Müller
Daniel
D
Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung (ZMBE), Von-Esmarch-Str. 56, D-48149 Münster, Germany.
Hagedorn
Peter
P
Brast
Sabine
S
Heusipp
Gerhard
G
Bielaszewska
Martina
M
Friedrich
Alexander W
AW
Karch
Helge
H
Schmidt
M Alexander
MA
D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D022603:Shiga Toxins / Q000096:biosynthesis*
D004269:DNA, Bacterial; D022603:Shiga Toxins