title: Crystallization and preliminary X-ray diffraction data of the complex between human centrin 2 and a peptide from the protein XPC.
abstract: Centrins are highly conserved calcium-binding proteins involved in the nucleotide-excision repair pathway as a subunit of the heterotrimer including the XPC and hHR23B proteins. A complex formed by a Ca2+-bound human centrin 2 construct (the wild type lacking the first 25 amino acids) with a 17-mer peptide derived from the XPC sequence (residues Asn847-Arg863) was crystallized. Data were collected to 1.65 angstroms resolution from crystals grown in 30% monomethyl polyethylene glycol (MPEG) 500, 100 mM NaCl and 100 mM Bicine pH 9.0. Crystals are monoclinic and belong to space group C2, with two molecules in the asymmetric unit. The unit-cell parameters are a = 60.28, b = 59.42, c = 105.14 angstroms, alpha = gamma = 90, beta = 94.67 degrees. A heavy-atom derivative was obtained by co-crystallization with Sr2+. The substitution was rationalized by calorimetry experiments, which indicate a binding constant for Sr2+ of 4.0 x 10(4) M(-1).
authors.affiliation: Laboratoire de Structure des Protéines, Département d'Ingénierie et d'Etude des Protéines, Commissariat à l'Energie Atomique CEA, 91191 Gif-sur-Yvette, France.
authors.forename: Jean Baptiste
authors.initials: JB
authors.lastname: Charbonnier
authors.forename: Petya
authors.initials: P
authors.lastname: Christova
authors.forename: Alexandra
authors.initials: A
authors.lastname: Shosheva
authors.forename: Enrico
authors.initials: E
authors.lastname: Stura
authors.forename: Marie Hélène
authors.initials: MH
authors.lastname: Le Du
authors.forename: Yves
authors.initials: Y
authors.lastname: Blouquit
authors.forename: Patricia
authors.initials: P
authors.lastname: Duchambon
authors.forename: Simona
authors.initials: S
authors.lastname: Miron
authors.forename: Constantin T
authors.initials: CT
authors.lastname: Craescu
mesh_terms: D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D002151:Calorimetry; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism*; D003460:Crystallization; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011503:Transglutaminases / Q000737:chemistry / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: C472752:CETN2 protein, human; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D010446:Peptide Fragments; C086984:XPC protein, human; D011503:Transglutaminases