Storage and oxidation of long-chain fatty acids in the C57/BL6 mouse heart as measured by NMR spectroscopy.
Triglyceride turnover in the isolated C57/BL6 mouse heart was measured by dynamic 13C edit-(1)H observe NMR and the rate of fatty acid oxidation was determined by 13C NMR isotopomer analysis. In the presence of a physiological mixture of substrates, energy was produced in the citric acid cycle by oxidation of long-chain fatty acids (18%), ketones (34%), lactate (24%), pyruvate (7%), and other sources (17%). Exogenous fatty acids appeared in the triglyceride pool at 0.24 micromol/g dry wt/min, similar to the rate of oxidation of long-chain fatty acids, 0.16 micromol/g dry wt/min. Isoproterenol decreased the rate of de novo triglyceride synthesis and increased the rate of fatty acid oxidation.
Stowe
Kimberly A
KA
Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Burgess
Shawn C
SC
Merritt
Matthew
M
Sherry
A Dean
AD
Malloy
Craig R
CR
D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology; D002952:Citric Acid Cycle / Q000187:drug effects / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism*; D006321:Heart / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology; D019344:Lactic Acid / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009924:Organ Culture Techniques; D010084:Oxidation-Reduction / Q000187:drug effects; D010477:Perfusion; D019289:Pyruvic Acid / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis
D002316:Cardiotonic Agents; D005227:Fatty Acids; D014280:Triglycerides; D019344:Lactic Acid; D019289:Pyruvic Acid; D007545:Isoproterenol