title: Immunological evaluation of bacterial derived Cochleate and proteoliposome as mucosal adjuvants.
abstract: We evaluated the potential of two bacterial derived compounds, Cochleate and Proteoliposome (PL), administrated to mice by nasal or oral routes on induction of antibody and cytokine responses. Anti PL IgG and IgA responses were measured by ELISA in saliva, sera or vaginal fluids of immunized mice. Productions of gammaIFN and IL-5 were determined in spleen cells of immunized mice following a recall in vitro with Cochleate or PL. Intranasal administration elicited a higher anti PL IgA response in both saliva and vaginal fluids as compared with oral route. Mice immunized with Cochleate or PL via intranasal or oral route-induced anti PL IgG and IgG2a antibody responses in their sera and vaginal fluids. Spleen cells from these immunized mice produced gammaIFN, but not IL-5, after a recall in vitro with Cochleate or PL. These results show that Cochleate and PL are capable of inducing both systemic and mucosal antibody responses as well as a Th1 type of immunity as evidenced by high gammaIFN and IgG2a antibody responses.
authors.affiliation: Immunology Department, Finlay Institute, P.O. Box 16017, Havana, Cuba. judithc@finlay.edu.cu
authors.forename: Judith
authors.initials: J
authors.lastname: del Campo
authors.forename: Miriam
authors.initials: M
authors.lastname: Lastre
authors.forename: Gustavo
authors.initials: G
authors.lastname: Bracho
authors.forename: Tamara
authors.initials: T
authors.lastname: Rodríguez
authors.forename: Danay
authors.initials: D
authors.lastname: Gil
authors.forename: Caridad
authors.initials: C
authors.lastname: Zayas
authors.forename: Carlos
authors.initials: C
authors.lastname: Taboada
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Acevedo
authors.forename: Danev Ricardo
authors.initials: DR
authors.lastname: Pérez
authors.forename: Oliver
authors.initials: O
authors.lastname: Pérez
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D005260:Female; D018928:Immunity, Mucosal; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D009345:Neisseria meningitidis; D011510:Proteolipids / Q000008:administration & dosage / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D011510:Proteolipids; C020485:proteoliposomes