UV radiation down-regulates Dsg-2 via Rac/NADPH oxidase-mediated generation of ROS in human lens epithelial cells.
Ultraviolet radiation-induced cataract has been believed to be associated with degradation of cellular components. We report that, in cultured human lens epithelial cells, UV radiation analogous to H2O2 treatment down-regulates desmosomal protein desmoglein-2. UV radiation induces generation of reactive oxygen species and transiently activates epidermal growth factor receptor, which in turn induces translocation of Rac2 and NADPH oxidase activity. Collectively, our data demonstrate that UV-induced desmoglein-2 down-regulation is mediated reactive oxygen species which are generated through EGFR activation and Rac2/NADPH oxidase activation, suggesting that antioxidants may be applied for protection against UV-induced cataract.
Jiang
Qin
Q
Department of Ophthalmology, Nanjing Medical University, Nanjing 210029, P.R. China.
Zhou
Changlin
C
Healey
Sarah
S
Chu
Wenming
W
Kouttab
Nicola
N
Bi
Zhigang
Z
Wan
Yinsheng
Y
D000818:Animals; D002460:Cell Line; D051301:Desmoglein 2 / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D004790:Enzyme Induction; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007908:Lens, Crystalline / Q000166:cytology / Q000528:radiation effects; D019255:NADPH Oxidases / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays; D020744:rac GTP-Binding Proteins / Q000378:metabolism*; D000097766:RAC2 GTP-Binding Protein
D051301:Desmoglein 2; D066246:ErbB Receptors; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases; D016877:Oxidants; D017382:Reactive Oxygen Species; D020744:rac GTP-Binding Proteins; D000097766:RAC2 GTP-Binding Protein; C495314:DSG2 protein, human