Impact of tributyltin and triphenyltin on ivory shell (Babylonia japonica) populations.
We histopathologically examined gonads and chemically determined organotin compounds in tissues of the ivory shell, Babylonia japonica. Imposex (a superimposition of male-type genital organs on females) occurred in approximately 80-90% of B. japonica specimens that we examined, with the penis and vas deferens both well developed. No oviduct blockage by vas deferens formation was observed. Ovarian spermatogenesis and suppressed ovarian maturation were observed in the females that exhibited imposex, although no histopathological abnormalities were found in males. Tissue distributions of organotin compounds [tributyltin (TBT), triphenyltin (TPhT), and their metabolites] were different for butyltins and phenyltins; a remarkably high accumulation of TBT was observed in the ctenidium, osphradium, and heart, whereas high concentrations of TPhT were detected in the ovary and digestive gland. More than one-third of TBT accumulated in the digestive glands of both males and females, followed by the testis, ctenidium, muscle, and heart tissues in males and in the muscle, ovary, ctenidium, and head tissues (including the central nervous system ganglia) in females. In both males and females, more than half of total TPhT accumulated in the digestive glands, followed by the gonads. The next highest values were in the muscle, ctenidium, and heart tissues in males and in the muscle, oviduct, and head tissues in females. Both TBT and TPhT concentrations in the gonads were positively correlated with penis length in females. Our findings strongly suggest that reproductive failure in adult females accompanied by imposex, possibly induced by TBT and TPhT from antifouling paints, may have caused the marked decline of B. japonica populations in Japan.
Horiguchi
Toshihiro
T
National Institute for Environmental Studies, Endocrine Disruptors and Dioxin Research Project, Ecological Effect Research Team, Tsukuba-shi, Ibaraki, Japan. thorigu@nies.go.jp
Kojima
Mitsuhiro
M
Hamada
Fumihiko
F
Kajikawa
Akira
A
Shiraishi
Hiroaki
H
Morita
Masatoshi
M
Shimizu
Makoto
M
D000818:Animals; D012734:Disorders of Sex Development / Q000139:chemically induced / Q000453:epidemiology; D005260:Female; D049851:Gastropoda / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects*; D007564:Japan; D008297:Male; D009929:Organ Size / Q000187:drug effects; D009947:Organotin Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D010053:Ovary / Q000166:cytology / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D014018:Tissue Distribution; D014220:Trialkyltin Compounds / Q000493:pharmacokinetics / Q000633:toxicity*
D009947:Organotin Compounds; D014220:Trialkyltin Compounds; C011559:tributyltin; C030665:triphenyltin