[Fluorescence resonance energy transfer quenching method for determination of arsenic with acridine orange-rhodamine B].
A new method for the determination of trace arsenic was developed by using fluorescence resonance energy transfer from acridine orange (AO) to rhodamine B (RB). It was found that under the condition of lambda(ex)/lambda(em) = 470/580 nm, effective energy transfer could occur between AO and RB in the dodecyl benzene sodium sulfonate solution. The fluorescence intensity of RB was diminished by molybdoarsenide which was formed by the reaction of arsenic (V) with molybdate in sulfuric acid medium. The detection limit of this method was 2. 56 microg x L(-1). This method was used for the determination of trace arsenic in tea. The range of determination for arsenic was 0.01-0.25 mg x L(-1). The relative standard deviation for the determination of arsenic was 0.48%-0.64%. The recoveries for the addition of 0.01-0.03 mg x L(-1) arsenic were 98%-103%. The method has been applied to the determination of arsenic with satisfactory results.
Liu
Bao-Sheng
BS
The Center of Physics and Chemistry, Hebei Provincial Key Laboratory of Analytical Science and Technology, Hebei University, Baoding 071002, China.
Gao
Jing
J
Liu
Zhi-Chao
ZC
Yu
Li-Na
LN
Yang
Da-Cheng
DC
Yang
Geng-Liang
GL
D000165:Acridine Orange / Q000737:chemistry; D001151:Arsenic / Q000032:analysis*; D028241:Camellia sinensis / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry
D005456:Fluorescent Dyes; D012235:Rhodamines; D000165:Acridine Orange; C029773:rhodamine B; D001151:Arsenic