Using capillary electrophoresis-selective tandem mass spectrometry to identify pathogens in clinical samples.
Analysis of microbial mixtures in complex systems, such as clinical samples, using mass spectrometry can be challenging because the specimens may contain mixtures of several pathogens or both pathogens and nonpathogens. We have successfully applied capillary electrophoresis-selective MS/MS of unique peptide marker ions to the identification of common pathogens in clinical diagnosis. We searched the CE-MS/MS spectra acquired from the proteolytic digests of pure bacterial cell extracts against protein databases. The identified peptides that matched a protein associated with a particular pathogen were selected as marker ions to identify that bacterium in clinical specimens. Thirty-four clinical specimens, obtained from pus, wound, sputum, and urine samples, were analyzed using both biochemical and selective MS/MS methods. The bacteria in these clinical samples were cultivated directly, without prior isolation of a pure colony, before performing the selective MS/MS analyses. The bacteria analyzed included both Gram-positive and -negative strains. The match with respect to the pathogens identified was good between the biochemical and the selective MS/MS methods; the matching rate was 91%. The rate was as high as 97% when not considering two specimens for which the bacteria were not grown successfully. Two of the specimens that we identified using the biochemical method as containing two bacterial species were confirmed also through selective tandem MS analysis.
Hu
Anren
A
Department of Chemistry, National Dong Hwa University, Hualien, Taiwan.
Chen
Cheng-Tung
CT
Tsai
Pei-Jen
PJ
Ho
Yen-Peng
YP
D019075:Electrophoresis, Capillary / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000472:pathogenicity*; D006094:Gram-Positive Bacteria / Q000737:chemistry* / Q000472:pathogenicity*; D053719:Tandem Mass Spectrometry / Q000379:methods*