[Molecular mapping of the gene(s) controlling petal-loss trait in Brassica napus].
An F2 population derived from a cross between apetalous line' APT02 'and normal petalled cultivar 'ZS NO.4' was used for molecular marker searching and chromosomal mapping of the gene(s) controlling petal-loss trait in Brassica napus. Twenty pairs of AFLP primers and 170 pairs of SRAP primers were selected and screened from two parents. In further selection through bulked segregant analysis (BSA) approach, one SRAP marker e8m3_4 (600 bp) and one AFLP marker E3247_15 (150 bp) were obtained and found to be linked to the gene(s) controlling petal-loss trait, with the genetic distance of 5 cM and 13.5 cM. A linkage map in Brassica napus was constructed. It consisted of 213 AFLP56 SSR loci and a morphology marker throughout 17 main linkage groups, two triplet and four linkage pairs. Total length of the map covered 2,487.1 cM, and average interval between markers was 10.09 cM. By genetic mapping, the gene(s) controlling petal-loss trait (WHB)was mapped in LG4.
Chen
Bi-Yun
BY
Oil Crops Research Institute of CAAS, Key Laboratory of Genetic Improvement of Oil Crops, Wuhan 430062, China. chenbiyun2008cn@yahoo.com.cn
Wu
Xiao-Ming
XM
Lu
Guang-Yuan
GY
Gao
Gui-Zhen
GZ
Xu
Kun
K
Li
Xiang-Zhi
XZ
D054458:Amplified Fragment Length Polymorphism Analysis; D029688:Brassica napus / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D035264:Flowers / Q000235:genetics* / Q000378:metabolism; D008040:Genetic Linkage; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D019655:Quantitative Trait, Heritable
D010940:Plant Proteins