title: Derivation and characterization of hepatocytes from embryonic stem cells in vitro.
abstract: From a therapeutic perspective on liver, the use of embryonic stem (ES) technology in the generation of a large number of high-functional hepatocytes developed from ES cells for cell transplantation is anticipated. We have explored a three-dimensional culture system in which hepatocytes differentiated from mouse ES cells by transfection with the hepatocyte nuclear factor-3beta possess high metabolic functions that can be maintained long term.
authors.affiliation: Program in Tissue Engineering, Department of Parasitology, Nara Medical University, Japan.
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ishizaka
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Ouji
authors.forename: Masahide
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakatani
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology*; D051379:Mice; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D015415:Biomarkers