Effect of titanium ion and resistance encoding plasmid of Pseudomonas aeruginosa ATCC 10145.
Titanium and its alloys are technically superior and cost-effective materials, with a wide variety of aerospace, industrial, marine, and commercial applications. In this study, the effects of titanium ions on bacterial growth were evaluated. Six strains of bacteria known to be resistant to both metal ions and antibiotics were used in this study. Two strains, Escherichia coli ATCC 15489, and Pseudomonas aeruginosa ATCC 10145, proved to be resistant to titanium ions. Plasmid-cured P. aeruginosa resulted in the loss of one or more resistance markers, indicating plasmid-encoded resistance. The plasmid profile of P. aeruginosa revealed the presence of a 23-kb plasmid. The plasmid was isolated and transformed into DH5alpha. Interestingly, the untransformed DH5alpha did not grow in 300 mg/l titanium ions, but the transformed DH5alpha grew quite well under such conditions. The survival rate of the transformed DH5alpha also increased more than 3-fold compared to that of untransformed DH5alpha.
Park
Sung Min
SM
Department of Microbiology, Keimyung University, Daegu 704-701, Republic of Korea.
Kim
Hyun Soo
HS
Yu
Tae Shick
TS
D003470:Culture Media; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D007477:Ions / Q000494:pharmacology*; D019565:Metals, Light / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D014025:Titanium / Q000494:pharmacology*; D014169:Transformation, Bacterial
D003470:Culture Media; D007477:Ions; D019565:Metals, Light; D014025:Titanium