title: Analysis of 13 fentanils, including sufentanil and carfentanil, in human urine by liquid chromatography-atmospheric-pressure ionization-tandem mass spectrometry.
abstract: A sensitive, semiautomated method for the analysis of several fentanils and fentanil metabolites in human urine is described. This method uses solid-phase extraction of urine samples followed by liquid chromatography-atmospheric pressure ionization-tandem mass spectrometry. Isotopically labeled internal standards were included for 6 of the 13 analytes in this study. Estimated detection limits ranged from 3 to 27 pg/mL, and good accuracy and long-term precision were observed in most cases. This method should be useful for the rapid and sensitive screening of human urine samples for a number of fentanils and their metabolites as indicators of exposure.
authors.affiliation: Emergency Response and Air Toxicants Branch, Division of Laboratory Science, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Mailstop F-47, Atlanta, Georgia 30341, USA.
authors.forename: Lanqing
authors.initials: L
authors.lastname: Wang
authors.forename: John T
authors.initials: JT
authors.lastname: Bernert
mesh_terms: D000701:Analgesics, Opioid / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005283:Fentanyl / Q000031:analogs & derivatives / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry
chemical_list: D000701:Analgesics, Opioid; D005283:Fentanyl