Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea.
A novel Tenacibaculum-like bacterial strain, SMK-4(T), was isolated from a tidal flat sediment in Korea. Strain SMK-4(T) was Gram-negative, pale yellow-pigmented and rod-shaped. It grew optimally at 30-37 degrees C and in the presence of 2-3 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C(15 : 0), iso-C(16 : 0) 3-OH and C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH as the major fatty acids (>10 % of total fatty acids). The DNA G+C content was 33.6 mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strain SMK-4(T) fell within the evolutionary radiation encompassed by the genus Tenacibaculum. Strain SMK-4(T) exhibited 16S rRNA gene sequence similarity levels of 95.2-98.6 % with respect to the type strains of recognized Tenacibaculum species. DNA-DNA relatedness levels and differential phenotypic properties made it possible to categorize strain SMK-4(T) as a species that is separate from previously described Tenacibaculum species. On the basis of phenotypic properties and phylogenetic and genetic distinctiveness, strain SMK-4(T) (=KCTC 12569(T)=JCM 13491(T)) should be classified as a novel Tenacibaculum species, for which the name Tenacibaculum aestuarii sp. nov. is proposed.
Jung
Seo-Youn
SY
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, South Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, South Korea.
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, South Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D041965:Flavobacteriaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D005840:Gentian Violet; D019015:Geologic Sediments / Q000382:microbiology*; D006131:Growth Inhibitors / Q000494:pharmacology; D007723:Korea; D008969:Molecular Sequence Data; D010619:Phenazines; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011809:Quinones / Q000032:analysis / Q000737:chemistry; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D012623:Seawater; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C035863:Gram's stain; D006131:Growth Inhibitors; D010619:Phenazines; D010860:Pigments, Biological; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; D005840:Gentian Violet