Comparison study of autonomous activity in bladders from normal and paraplegic rats.
OBJECTIVE
To identify differences in the pattern of pressure generated by isolated bladders from normal and paraplegic rats.


METHODS
Nine female Wister rats were made paraplegic by spinal cord transsection at the vertebral level T8-T9 and sacrificed between D21 and D28. A further group (n = 9) was used as a control group. Each bladder was excised and placed in an organ bath where intravesical pressures were measured. Pressure changes were divided in two well-defined groups: macro-transients and spikes. The effects of intravesical volume load and muscarinic (M) agonists were studied.


RESULTS
We demonstrated a higher frequency, a longer duration, and a higher variance of duration in macro-transients in the neurogenic group. Intravesical volume load influenced the amplitude and frequency of macro-transients in both groups similarly. The effects of the muscarinic (M(2))-selective agonist arecaïdine were different in neurogenic bladder; the effects of the non-selective muscarinic (M)-agonist carbachol were similar in both groups.


CONCLUSIONS
We showed that the pattern of autonomous activity was significantly different between normal and neurogenic rat bladders. We also found evidence for alterations in the muscarinic response of isolated neurogenic rat bladders. This model offers an exciting new research tool to evaluate the detrusor activity in neurogenic and normal conditions.
Gevaert
Thomas
T
KU Leuven, Urology, Leuven, Belgium.
Ost
Dieter
D
De Ridder
Dirk
D
D000818:Animals; D001115:Arecoline / Q000031:analogs & derivatives / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D018679:Cholinergic Agonists / Q000494:pharmacology; D005260:Female; D018721:Muscarinic Agonists / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D010264:Paraplegia / Q000150:complications / Q000503:physiopathology*; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology*; D001750:Urinary Bladder, Neurogenic / Q000209:etiology / Q000503:physiopathology*; D014563:Urodynamics / Q000187:drug effects / Q000502:physiology
D018679:Cholinergic Agonists; D018721:Muscarinic Agonists; C015688:arecaidine; D001115:Arecoline; D002217:Carbachol