title: Phylogenetic relationship of Pasteurella pneumotropica isolates from laboratory rodents based on 16S rDNA sequence.
abstract: A 1344 bp fragment of the 16S ribosomal DNA (rDNA) sequence was used to determine the genetic relationship of Pasteurella pneumotropica isolates from laboratory rodents. A total of 30 nucleotide sequences of P. pneumotropica, including 24 wild strains, 3 reference strains, and 3 nucleotide sequences deposited in GenBank, were examined for heterogeneity of their 16S rDNA sequences. Phylogenetic analysis based on 16S rDNA sequence discriminated 5 types of branching lineages. Of these 5 types, 3 types had significant associations with mice or rats, and 2 had significant associations with the beta-hemolytic phenotype. These results suggest that 16S rDNA sequencing of P. pneumotropica isolates demonstrates genetic heterogeneity and phylogenetic discrimination in terms of their hemolytic phenotype and host associations.
authors.affiliation: Animal Research Center, Tokyo Medical University, Japan.
authors.forename: Hiraku
authors.initials: H
authors.lastname: Sasaki
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kawamoto
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Ueshiba
authors.forename: Hiromi
authors.initials: H
authors.lastname: Amao
authors.forename: Takuo
authors.initials: T
authors.lastname: Sawada
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D044142:Pasteurella pneumotropica / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D051381:Rats
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S