Effect of the visible light irradiation of fullerene-containing composites on the ROS generation and the viability of tumor cells.
OBJECTIVE
To study the effect of fullerene-containing composites, irradiated by visible light, on the radical oxygen species (ROS) generation in thymocytes, ascitic cells from Erlich's tumor and leukemia cells L1210; to investigate viability of these cells in the presence of fullerene-containing composites under irradiation conditions.


METHODS
The viability of cells was evaluated by staining with 0.4% solution of the trypan blue; ROS were detected with the use of electron paramagnetic resonance (EPR) spectroscopy and spin traps; solutions of fullerene-containing composites were irradiated with mercury-vapor lamp.


RESULTS
We demonstrated that under irradiation conditions fullerene-containing composites increase the rate of ROS generation and decrease the number of viable tumor cells.


CONCLUSIONS
The obtained data allow to consider the fullerene-containing composites as potential agents for photodynamic therapy.
Prylutska
S V
SV
Kyiv National Shevchenko University, Kyiv, Ukraine. prylut@biocc.univ.kiev.ua
Burlaka
A P
AP
Matyshevska
O P
OP
Golub
A A
AA
Potebnya
G P
GP
Prylutskyy
Y I
YI
Ritter
U
U
Scharff
P
P
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000378:metabolism; D037741:Fullerenes / Q000494:pharmacology / Q000528:radiation effects / Q000627:therapeutic use*; D007938:Leukemia / Q000188:drug therapy* / Q000378:metabolism; D008027:Light; D010778:Photochemotherapy; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism
D037741:Fullerenes; D017382:Reactive Oxygen Species