Morphology of Malassez's epithelial rest-like cells in the cementum: transmission electron microscopy, immunohistochemical, and TdT-mediated dUTP-biotin nick end labeling studies.
OBJECTIVE
It is known that epithelial islands are embedded in the cementum during tooth root formation, but details of this process remain unknown. The purpose of this study was to investigate the dynamic characteristics of Malassez's epithelial rest cells in the cementum during tooth root formation in pigs in vivo.


METHODS
The first molars of 6-mo-old pigs were used in this study. Specimens were decalcified before being embedded in paraffin. Paraffin sections were investigated using TdT-mediated dUTP-biotin nick end labeling (TUNEL), immunohistochemical, and ultrastructural techniques.


RESULTS
Malassez's epithelial rest cells were located close to the root surface at the apical one-third of the periodontal ligament, and epithelial clusters surrounded by distinct lamina cementia were sometimes observed in the cementum. TUNEL-positive cells were detected only in the cementum. Malassez's epithelial rest cells in the periodontal ligament were completely surrounded by basement membranes, but epithelial clusters in the cementum were only intermittently surrounded by such membranes. Cytokeratin-positive cells in the superstratum of the cementum were directly connected by cementocytes and by desmosome-like structures. However, organelles were scarce in the cytokeratin-positive cells in the substratum of the cementum, and the matrix of the cementum was deposited in the cells.


CONCLUSIONS
These results suggest that the majority of the fragmented Hertwig's root sheath remains in the periodontal ligament and that some cells, which are connected to cementoblasts, are embedded in the cementum and progress to apoptosis.
Suzuki
M
M
Oral Health Science Center and Department of Periodontics, Tokyo Dental College, Chiba, Japan.
Matsuzaka
K
K
Yamada
S
S
Shimono
M
M
Abiko
Y
Y
Inoue
T
T
D000818:Animals; D017209:Apoptosis; D025021:Cementogenesis; D003739:Dental Cementum / Q000166:cytology*; D004847:Epithelial Cells / Q000648:ultrastructure*; D007124:Immunoenzyme Techniques; D020287:In Situ Nick-End Labeling; D046529:Microscopy, Electron, Transmission; D013552:Swine