Highly efficient capture and long-term encapsulation of dye by catanionic surfactant vesicles.
Vesicles formed from the cationic surfactant, cetyltrimethylammonium tosylate (CTAT) and the anionic surfactant, sodium dodecylbenzenesulfonate (SDBS), were used to sequester the anionic dye carboxyfluorescein. Carboxyfluorescein was efficiently sequestered in CTAT-rich vesicles via two mechanisms: encapsulation in the inner water pool and electrostatic adsorption to the charged bilayer. The apparent encapsulation efficiency (22%) includes both encapsulated and adsorbed fractions. Entrapment of carboxyfluorescein by SDBS-rich vesicles was not observed. Results show the permeability of the catanionic membrane is an order of magnitude lower than that of phosphatidylcholine vesicles and the loading capacity is more than 10 times greater.
Wang
Xiang
X
Departments of Chemistry and Biochemistry, and Chemical & Biomolecular Engineering, University of Maryland, College Park, MD 20742-2111, USA.
Danoff
Emily J
EJ
Sinkov
Nikolai A
NA
Lee
Jae-Ho
JH
Raghavan
Srinivasa R
SR
English
Douglas S
DS
D001557:Benzenesulfonates / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D002593:Cetrimonium Compounds / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D013997:Time Factors
D001557:Benzenesulfonates; D002412:Cations; D002593:Cetrimonium Compounds; D005452:Fluoresceins; D013501:Surface-Active Agents; C020810:trimethylcetylammonium p-toluenesulfonate; C024098:6-carboxyfluorescein; C001114:dodecylbenzenesulfonic acid