title: In vitro effects of aminobisphosphonates on Vgamma9Vdelta2 T cell activation and differentiation.
abstract: In this study we have evaluated the in vitro effects of four different aminobisphosphonates, alendronate, risedronate, neridronate and zoledronate, on Vgamma9Vdelta2 T cell activation and differentiation. All tested aminobisphosphonates induce an IL-2-dependent activation and expansion of Vgamma9Vdelta2 T lymphocytes in primary PBMC cultures of healthy donors. Most notably, they also determine a different distribution of Vgamma9Vdelta2 T cell subsets, with decrease of T(naive) and T(CM) cells and increase of T(EM) and T(EMRA) Vgamma9Vdelta2cells, indicating that in vitro treatment with aminobisphosphonates induces Vgamma9Vdelta2 T lymphocytes to differentiate towards an effector/cytotoxic phenotype. Accordingly, Vgamma9Vdelta2 T lymphocytes cultured with aminobisphosphonates and IL-2 showed a major content of IFN-gamma and acquired the ability to kill tumor target cells.
authors.affiliation: Dept. of Biopathology and Biomedical Methods, University of Palermo, 90134 Palermo, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Ferlazzo
authors.forename: C
authors.initials: C
authors.lastname: Sferrazza
authors.forename: N
authors.initials: N
authors.lastname: Caccamo
authors.forename: G
authors.initials: G
authors.lastname: Di Fede
authors.forename: G
authors.initials: G
authors.lastname: Di Lorenzo
authors.forename: M
authors.initials: M
authors.lastname: D'Asaro
authors.forename: S
authors.initials: S
authors.lastname: Meraviglia
authors.forename: F
authors.initials: F
authors.lastname: Dieli
authors.forename: G
authors.initials: G
authors.lastname: Rini
authors.forename: A
authors.initials: A
authors.lastname: Salerno
mesh_terms: D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004164:Diphosphonates / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D019044:Image Cytometry; D007371:Interferon-gamma / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D009000:Monocytes / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects*
chemical_list: D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D007371:Interferon-gamma