title: A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.
abstract: Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.
authors.affiliation: J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA. sgoldberg@venterinstitute.org
authors.forename: Susanne M D
authors.initials: SM
authors.lastname: Goldberg
authors.forename: Justin
authors.initials: J
authors.lastname: Johnson
authors.forename: Dana
authors.initials: D
authors.lastname: Busam
authors.forename: Tamara
authors.initials: T
authors.lastname: Feldblyum
authors.forename: Steve
authors.initials: S
authors.lastname: Ferriera
authors.forename: Robert
authors.initials: R
authors.lastname: Friedman
authors.forename: Aaron
authors.initials: A
authors.lastname: Halpern
authors.forename: Hoda
authors.initials: H
authors.lastname: Khouri
authors.forename: Saul A
authors.initials: SA
authors.lastname: Kravitz
authors.forename: Federico M
authors.initials: FM
authors.lastname: Lauro
authors.forename: Kelvin
authors.initials: K
authors.lastname: Li
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Rogers
authors.forename: Robert
authors.initials: R
authors.lastname: Strausberg
authors.forename: Granger
authors.initials: G
authors.lastname: Sutton
authors.forename: Luke
authors.initials: L
authors.lastname: Tallon
authors.forename: Torsten
authors.initials: T
authors.lastname: Thomas
authors.forename: Eli
authors.initials: E
authors.lastname: Venter
authors.forename: Marvin
authors.initials: M
authors.lastname: Frazier
authors.forename: J Craig
authors.initials: JC
authors.lastname: Venter
mesh_terms: D001709:Biotechnology / Q000379:methods* / Q000639:trends; D019295:Computational Biology / Q000379:methods; D020451:Contig Mapping; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D017422:Sequence Analysis, DNA / Q000379:methods*