title: Use of filter paper stored dried blood for measurement of triglycerides.
abstract: Adaptation of assays on dried blood has advantages of ease of collection, transportation, minimal invasiveness and requirement of small volume. A method for extraction and estimation of triglyceride from blood spots dried on filter paper (Whatman no. 3) has been developed. A single dried blood spot containing 10 muL blood was used. Triglyceride was efficiently extracted in methanol from blood dried on filter paper by incubation at 37 degrees C for two hours with gentle shaking. For the estimation, a commercially available enzymatic method was used. Blood spot assays showed mean intra and inter assay coefficient of variance of 6.0% and 7.4% respectively. A comparison of paired whole blood spots and plasma samples (n = 75, day 0) gave an intraclass correlation of 0.96. The recovery was 99.6%. The dried blood triglyceride concentrations were stable for one month when the filter discs were stored at room temperature (16-28 degrees C). Storage of filters at 4 degrees C extended the stability and triglycerides could be quantitatively recovered after 3 months of storage.
authors.affiliation: Laboratory Medicine department, All India Institute of Medical Sciences (AIIMS), New Delhi, India. rizandaiims@yahoo.co.in
authors.forename: Rizwana
authors.initials: R
authors.lastname: Quraishi
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Lakshmy
authors.forename: Dorairaj
authors.initials: D
authors.lastname: Prabhakaran
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Mukhopadhyay
authors.forename: Bansilal
authors.initials: B
authors.lastname: Jailkhani
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods*; D003080:Cold Temperature; D005374:Filtration; D006801:Humans; D010209:Paper; D014280:Triglycerides / Q000097:blood*
chemical_list: D014280:Triglycerides