title: Effect of direct infusion of acetaldehyde on dopamine and dopamine-derived salsolinol in the striatum of free-moving rats using a reverse microdialysis technique.
abstract: The effects of acetaldehyde (ACD) on dopamine (DA) and DA-derived salsolinol (SAL) levels were investigated in the striatum of freely moving rats. Dialysate levels of DA and SAL were determined using in vivo reverse microdialysis coupled with high-performance liquid chromatography with an electrochemical detector. Perfusion with 1,000 microM ACD decreased DA levels significantly, as compared to baseline value, whereas 250 and 500 microM ACD perfusion did not result in any significant alteration of the DA levels in the striatal dialysates. SAL levels in the dialysates were determined first at 30 or 40 min after ACD perfusion, reached a peak at 150 min, followed by no alterations for 240 min with doses of 250, 500, and 1,000 microM ACD. Our in vivo study suggested that 1,000 microM ACD led to significant decreases in DA levels in the striatum with greater SAL formation, and the examined ACD concentrations induced a dose-dependent elevation in SAL levels in the striatum of freely moving rats.
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan. weihuanw@kms.ac.jp
authors.forename: Weihuan
authors.initials: W
authors.lastname: Wang
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ameno
authors.forename: Mostofa
authors.initials: M
authors.lastname: Jamal
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Kumihashi
authors.forename: Ikuo
authors.initials: I
authors.lastname: Uekita
authors.forename: Setsuko
authors.initials: S
authors.lastname: Ameno
authors.forename: Iwao
authors.initials: I
authors.lastname: Ijiri
mesh_terms: D000079:Acetaldehyde / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects*; D004298:Dopamine / Q000494:pharmacology*; D004347:Drug Interactions; D004359:Drug Therapy, Combination; D007546:Isoquinolines / Q000494:pharmacology*; D008297:Male; D017551:Microdialysis / Q000379:methods; D009068:Movement / Q000502:physiology; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007546:Isoquinolines; C036617:salsolinol; D000079:Acetaldehyde; D004298:Dopamine