Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells.
We describe here the isolation and maintenance of pluripotent embryonic stem (ES) cells from equine blastocysts that have been frozen and thawed. Equine ES cells appear to maintain a normal diploid karyotype in culture. These cells express markers that are characteristic of mouse ES cells, namely, alkaline phosphatase, stage-specific-embryonic antigen 1, STAT3, and Oct4. We also describe protocols for the induction of differentiation in vitro to neural precursor cells in the presence of basic fibroblast growth factor (bFGF), epidermal growth factor, and platelet-derived growth factor and to hematopoietic and endothelial cell lineages in the presence of bFGF, stem cell factor, and oncostatin M. Equine ES cells provide a powerful tool for gene targeting and the generation of transgenic clonal offspring.
Saito
Shigeo
S
Saito Laboratory of Cell Technology, Kataoka, Yaita, Techigi, Japan.
Sawai
Ken
K
Minamihashi
Arika
A
Ugai
Hideyo
H
Murata
Takehide
T
Yokoyama
Kazunari K
KK
D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002469:Cell Separation; D018920:Coculture Techniques; D015925:Cryopreservation; D003470:Culture Media; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D006736:Horses / Q000196:embryology*; D007621:Karyotyping; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003470:Culture Media; D012313:RNA; D004247:DNA