[Separation of enantiomers of amino acid derivatives by high performance liquid chromatography on teicoplanin chiral stationary phase].
Direct enantioseparations of seven N-fluorenylmethoxycarbonyl-amino acids (Fmoc-Val, Fmoc-Ser, Fmoc-Leu, Fmoc-Ile, Fmoc-Met, Fmoc-Ala, and Fmoc-Phe) were achieved by high performance liquid chromatography using a 250 mm x 4. 6 mm i.d. column bonded teicoplanin on silica gel as chiral stationary phase and methanol-acetic acid-triethylamine mixtures as mobile phase. The comparison of retention behavior and enantioseparation of N-Fmoc-Val and its non-blocked analogue on a Chirobiotic column with different mobile phase systems is presented. The effects of the content of acetic acid and triethylamine and the content ratio of acetic acid and triethylamine in the mobile phase on the enantioseparation of some N-Fmoc-amino acids were examined. The results showed that variations of column temperatureand flow rate of mobile phase also had effects on enantioseparation of N-Fmoc-amino acids. The method had been successfully applied to the determination of optical purity of some derivatized amino acids.
Lin
Lin
L
Shanghai Institute of Organic Chemistry, the Chinese Academy of Sciences, Shanghai 200032, China.
Xia
Lijun
L
Xu
Xu
X
Xu
Hongyan
H
D000596:Amino Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019411:Clinical Laboratory Techniques; D005449:Fluorenes / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013237:Stereoisomerism; D017334:Teicoplanin / Q000737:chemistry*; D013696:Temperature
D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D017334:Teicoplanin