Identification of genes of the plant-specific transcription-factor families cooperatively regulated by ethylene and jasmonate in Arabidopsis thaliana.
The analysis of expression patterns of transcription-factor genes will be the basis for a better understanding of their biological functions in plants. In this study, we designed and developed an oligo-DNA macroarray consisting of gene-specific probes of 60-65 nucleotides for 288 transcription-factor genes, which cover COL, DOF, ERF, and NAC family genes. To investigate transcription-factor genes that are cooperatively regulated by jasmonate and ethylene in arabidopsis (Arabidopsis thaliana (L.) Heynh.) plants, we analyzed the expression profile of transcription-factor genes using the oligo-DNA macroarray technique in arabidopsis plants treated with methyl jasmonate and 1-aminocyclopropane-1-carboxylic acid. Then, transcript levels of candidate genes-which were selected based on the result of macroarray analysis-were evaluated by the quantitative real-time RT-PCR method. Finally, we identified an ERF family gene that is cooperatively regulated by both hormones, and designated as cooperatively regulated by ethylene and jasmonate 1 (CEJ1).
Nakano
Toshitsugu
T
Molecular and Cellular Breeding Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Suzuki
Kaoru
K
Ohtsuki
Namie
N
Tsujimoto
Yayoi
Y
Fujimura
Tatsuhito
T
Shinshi
Hideaki
H
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics*; D003517:Cyclopentanes / Q000494:pharmacology*; D005030:Ethylenes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects
D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D014157:Transcription Factors; C011006:jasmonic acid; C036216:ethylene