Use of allogenic epidermal sheets for difficult wound healing: selection and testing of relevant growth factors.
The clinical interest of using allogenic epidermal sheets (AES) has largely been shown [1,2,3]. As well as covering, they also stimulate healing, by simultaneously secreting numerous growth factors (GFs), although little is known on their mechanism of action. Our objectives were to: (a) devise a test for the efficacy of AES release, (b) select keratinocyte-secreting strains and optimal culture conditions. Three GFs were selected: IL-1alpha, IL-8 and VEGF. Three different keratinocyte strains were cultured for 3 and 6 days after confluence for 3 passages. Assays were performed after 3 h and 24 h+3 h after dispase treatment (AES conservation for 24 h then change of medium and sampling after 3 h). AES were found to secrete GFs in DMEM and the amounts were greater when cultured for 6 rather than 3 days after confluence. Each strain had different secretory patterns depending on passage and time in culture, this variability being explained by inter-individual heterogeneity.
Auxenfans
C
C
Banque de Tissus et Cellules des Hospices Civils de Lyon, Hôpital E. Herriot, 69437 Lyon, Rhône, France.
Colloud
M
M
Debard
A L
AL
Braye
F M
FM
Amini
M
M
Allombert-Blaise
V
V
Builles
N
N
Claudy
A
A
Damour
O
O
D049109:Cell Proliferation; D002478:Cells, Cultured; D004817:Epidermis / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D053582:Interleukin-1alpha / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology; D016038:Skin Transplantation / Q000379:methods*; D013997:Time Factors; D014456:Ulcer / Q000628:therapy; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing
D036341:Intercellular Signaling Peptides and Proteins; D053582:Interleukin-1alpha; D016209:Interleukin-8; D042461:Vascular Endothelial Growth Factor A