Halorubrum lipolyticum sp. nov. and Halorubrum aidingense sp. nov., isolated from two salt lakes in Xin-Jiang, China.
Two extremely halophilic archaea, strains 9-3(T) and 31-hong(T), were isolated from Aibi salt lake and Aiding salt lake in Xin-Jiang, China. Their morphology, physiology, biochemical features, polar lipid compositions and 16S rRNA gene sequences were characterized in order to elucidate their taxonomic status. The results from this study indicated that strains 9-3(T) and 31-hong(T) are members of the genus Halorubrum. Their physiological properties and polar lipid compositions are clearly different from those of the currently described species of Halorubrum. DNA-DNA relatedness values for strain 9-3(T) with respect to its closely related neighbours Halorubrum saccharovorum JCM 8865(T) and Halorubrum lacusprofundi JCM 8891(T) were 51.6 and 25.1 %, respectively, DNA-DNA relatedness values for strain 31-hong(T) with respect to its closely related neighbours Hrr. saccharovorum JCM 8865(T) and Hrr. lacusprofundi JCM 8891(T) were 29.4 and 44.9 %, respectively, and DNA-DNA relatedness between strains 9-3(T) and 31-hong(T) was 54 %. Thus, two novel species of the genus Halorubrum are proposed, Halorubrum lipolyticum sp. nov. (type strain 9-3(T)=CGMCC 1.5332(T)=JCM 13559(T)) and Halorubrum aidingense sp. nov. (type strain 31-hong(T)=CGMCC 1.2670(T)=JCM 13560(T)).
Cui
Heng-Lin
HL
Graduate University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, People's Republic of China.
Tohty
Dilbr
D
College of Life Sciences, Xin-Jiang Normal University, Urumqi 830053, People's Republic of China.
Zhou
Pei-Jin
PJ
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, People's Republic of China.
Liu
Shuang-Jiang
SJ
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, People's Republic of China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D008055:Lipids; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S