title: Electrochemical biosensor evaluation of the interaction between DNA and metallo-drugs.
abstract: Electrochemical techniques were used to study the interaction between a panel of antiproliferative metallo-drugs and double-stranded DNA immobilized on screen-printed electrodes as a model of the analogous interaction occurring in solution. The propensity of a given metal drug to interact with DNA was measured as a function of the decrease of guanine oxidation signal, which was detected by square wave voltammetry. Estimates of variations in experimental parameters, such as the concentration of complexes, time following dissolution (ageing time) and the presence of chloride, are provided.
authors.affiliation: Dipartimento di Chimica, Università di Firenze, Polo Scientifico Universitario, Via della Lastruccia 3, 50019, Sesto Fiorentino, FI, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Mascini
authors.forename: Graziana
authors.initials: G
authors.lastname: Bagni
authors.forename: Maria Letizia
authors.initials: ML
authors.lastname: Di Pietro
authors.forename: Mauro
authors.initials: M
authors.lastname: Ravera
authors.forename: Sara
authors.initials: S
authors.lastname: Baracco
authors.forename: Domenico
authors.initials: D
authors.lastname: Osella
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006147:Guanine / Q000737:chemistry; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000187:drug effects
chemical_list: D003432:Cross-Linking Reagents; D006147:Guanine; D004247:DNA; D002945:Cisplatin