title: Evaluation of INNO-LiPA assay for direct detection of mycobacteria in pulmonary and extrapulmonary specimens.
abstract: The INNO-LiPA Mycobacteria kit has been developed for detecting mycobacteria in liquid and solid cultures through amplification of the 16S-23S rRNA mycobacterial spacer region and the use of species-specific probes. The aim of this study was to verify the possible direct use of the kit on clinical samples. The study was performed retrospectively on a total of 129 specimens (104 pulmonary and 25 extrapulmonary) and the results were compared to those obtained from culture. For pulmonary specimens, the overall clinical sensitivity of INNO-LiPA Mycobacteria kit was 79.5% and its specificity 84.6%. For extrapulmonary samples, the kit had an overall clinical sensitivity of 71.4%. In both cases no false positive results were found.
authors.affiliation: Department of Experimental and Applied Medicine, Microbiology and Virology, University of Brescia, Italy. perandin@med.unibs.it
authors.forename: Francesca
authors.initials: F
authors.lastname: Perandin
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pinsi
authors.forename: Costantino
authors.initials: C
authors.lastname: Signorini
authors.forename: Nino
authors.initials: N
authors.lastname: Manca
mesh_terms: D006801:Humans; D008168:Lung / Q000382:microbiology*; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D011934:Reagent Strips; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D011934:Reagent Strips