An apparatus for studying the response of cultured endothelial cells to stresses.
In order to simulate the hemodynamic environment of human arteries in vivo, we designed a laminar flow apparatus which can precisely simulate the normal stress and shear stress to which cultured endothelial cells are exposed. Under both normal and abnormal physiological conditions, this apparatus can accurately control and adjust the values of normal stress and shear stress and the frequency of pulse waves, as well as the amplitude of pulsatile flow. This in vitro apparatus provides an experimental platform for studying the response of the biological characteristics of cultured endothelial cells in a hemodynamic environment.
Shen
L
L
Biomechanics & Medical Information Institute, Beijing University of Technology, China.
Qiao
A
A
Ding
H
H
Mo
G
G
Xu
G
G
Du
Y
Y
Li
M
M
Chen
Z
Z
Zeng
Y
Y
D001783:Blood Flow Velocity / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D010812:Physical Stimulation / Q000295:instrumentation* / Q000379:methods; D011673:Pulsatile Flow / Q000502:physiology; D033081:Shear Strength; D013314:Stress, Mechanical; D014159:Transducers