title: Selective identification of Valpha14i T cells using slide-immobilized, CD1d-antigen complexes.
abstract: The ability to correlate changes in antigen-reactive lymphocytes with disease will provide information needed to develop strategies for combating illness. One critical group of lymphocytes are the CD1-restricted T cells. It is desirable to use CD1 molecules in an array format to query CD1-restricted lymphocytes in humans. To investigate the feasibility of this technique, we employed mCD1d and alpha-galactosylceramide to demonstrate that-slide immobilized, CD1d-alpha-GalCer complexes capture an NKT cell hybridoma in the presence of a competitor. The success of this scheme represents the first step toward the development of CD1-antigen arrays that could be used to profile biological samples.
authors.affiliation: Department of Biological Sciences and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907-2054, USA.
authors.forename: Alfred J
authors.initials: AJ
authors.lastname: Zullo
authors.forename: Randy R
authors.initials: RR
authors.lastname: Brutkiewicz
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Taparowsky
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000276:immunology*; D055610:Antigens, CD1d; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D018949:Antigens, CD1; D055610:Antigens, CD1d; C526877:CD1D protein, human