Rhodnius prolixus: identification of immune-related genes up-regulated in response to pathogens and parasites using suppressive subtractive hybridization.
We report the identification of immune-related molecules from the fat body, and intestine of Rhodnius prolixus, an important vector of Chagas disease. Insects were challenged by introducing pathogens or Trypanosoma cruzi, the parasite that causes Chagas disease, into the hemocoel. RNA from intestines, or fat body were isolated 24h after stimulation. We used suppressive subtractive hybridization to identify immune-related genes, generated three subtracted libraries, sequenced the clones and assembled the sequences. The functional annotation revealed expressed sequence tags (ESTs) generated in response to various stimuli in all tissues, and included pathogen recognition molecules, regulatory molecules, and effector molecules.
Ursic-Bedoya
Raul J
RJ
Department of Biological Sciences, Simon Fraser University, 8888 University Dr., Burnaby, BC, Canada V5A1S6. rursicbe@sfu.ca
Lowenberger
Carl A
CA
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics; D014355:Chagas Disease / Q000635:transmission*; D020224:Expressed Sequence Tags; D005216:Fat Body / Q000378:metabolism; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000378:metabolism; D009077:Mucins / Q000235:genetics; D009569:Nitric Oxide / Q000502:physiology; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012239:Rhodnius / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology; D014168:Transferrin / Q000235:genetics; D015854:Up-Regulation
D023181:Antimicrobial Cationic Peptides; D009077:Mucins; D014168:Transferrin; D009569:Nitric Oxide