title: [Cytotoxic T lymphocyte antigen-4 promoter gene polymorphism is significantly associated with ulcerative colitis].
abstract: OBJECTIVE
  Inflammatory bowel disease (IBD) is characterized by the T-cell excessive activation of intestinal mucosa. Cytotoxic T-lymphocyte associated antigen 4 (CTLA-4) is a negative regulator of T-lymphocyte activation. The aim of the present study is to investigate the association between CTLA-4 promoter -1722 (T/C), -1661 (A/G) polymorphisms and ulcerative colitis (UC) in Han Chinese, in Hubei province of central China.
  
  
  METHODS
  Eighty-seven patients with UC and 116 healthy controls were genotyped for CTLA-4 promoter -1722 and -1661 polymorphisms with a method of polymerase chain reaction based restriction fragment length polymorphism.
  
  
  RESULTS
  The frequency of "A/G + G/G" genotype at the -1661 site was statistically higher in UC patients than in healthy controls (34.5% vs 15.5%, P = 0.002, OR = 2.865, 95% CI = 1.467-5.596). The frequency of the G allele at the -1661 site was also significantly higher in UC patients than in the controls (19.0% vs 8.2%, P = 0.002, OR = 2.624, 95% CI = 1.435-4.796). However, the distribution of the genotypes at -1722 site was not significantly different between the UC patients and the controls.
  
  
  CONCLUSIONS
  The G allele of CTLA-4 promoter -1661 polymorphism showed a highly significant association with UC in Han Chinese of the central China.
authors.affiliation: Department of Internal Medicine, Research Center of Digestive Diseases, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.forename: Qiu-sha
authors.initials: QS
authors.lastname: Guo
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Hong
authors.initials: H
authors.lastname: Cheng
mesh_terms: D000328:Adult; D000483:Alleles; D015703:Antigens, CD / Q000235:genetics*; D000943:Antigens, Differentiation / Q000235:genetics*; D060908:CTLA-4 Antigen; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000276:immunology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human