title: Adverse effect of chloride impurities on lipase-catalyzed transesterifications in ionic liquids.
abstract: The adverse influence of chloride impurities on the lipase-catalyzed transesterification in ionic liquid is described. The activity of lipase from Rhizomucor miehei exponentially decreased with increasing Cl(-) content in 1-octyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl] amide, [Omim][Tf(2)N], and the activity of lipase in [Omim][Tf(2)N] mixture containing 2% [Omim] [Cl] was only about 2% of the activity in pure [Omim][Tf(2)N]. The activity of lipase from Candidantarctica linearly decreased at about 5% with every 1% increase in [Omim][Cl] with there being no activity in [Omim][Tf(2)N] containing about 20% [Omim][Cl].
authors.affiliation: Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Ha
authors.forename: Sun Bok
authors.initials: SB
authors.lastname: Lee
authors.forename: Yoon-Mo
authors.initials: YM
authors.lastname: Koo
mesh_terms: D002384:Catalysis; D002712:Chlorides / Q000494:pharmacology*; D004795:Enzyme Stability; D004951:Esterification; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D020103:Rhizomucor / Q000201:enzymology*
chemical_list: D002712:Chlorides; D007093:Imidazoles; D052578:Ionic Liquids; D008049:Lipase