Separation of triacylglycerols in a complex lipidic matrix by using comprehensive two-dimensional liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometric detection.
The present investigation describes the employment of a comprehensive 2-D HPLC system, based on the combination of a silver ion and an RP column, for the characterization of the triacylglycerol (TAG) fraction of a very complex lipidic sample: donkey milk fat. The TAGs were grouped on the resulting bidimensional contour plot according to their double bond numbers (aligned along vertical bands) and according to their partition numbers (aligned along horizontal bands). Peak assignment was supported by using atmospheric pressure chemical ionization mass spectrometric (APCI-MS) detection. The combination of the enhanced resolving power of comprehensive multidimensional LC, the formation of ordered 2-D patterns, and APCI-MS detection proved to be an effective tool for the characterization of the complex matrix, enabling the separation and identification of nearly 60 TAGs.
Dugo
Paola
P
Dipartimento di Chimica Organica e Biologica, Facoltà di Scienze, Università di Messina, Italy.
Kumm
Tiina
T
Chiofalo
Biagina
B
Cotroneo
Antonella
A
Mondello
Luigi
L
D000818:Animals; D001274:Atmospheric Pressure; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D018397:Equidae; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008892:Milk / Q000737:chemistry; D014280:Triglycerides / Q000302:isolation & purification*
D014280:Triglycerides