Denitratisoma oestradiolicum gen. nov., sp. nov., a 17beta-oestradiol-degrading, denitrifying betaproteobacterium.
A Gram-negative, motile, denitrifying bacterium (strain AcBE2-1(T)) was isolated from activated sludge of a municipal wastewater treatment plant using 17beta-oestradiol (E2) as sole source of carbon and energy. Cells were curved rods, 0.4-0.8 x 0.8-2.0 microm in size, non-fermentative, non-spore-forming, oxidase-positive and catalase-negative. E2 was oxidized completely to carbon dioxide and water by reduction of nitrate to a mixture of dinitrogen monoxide and dinitrogen, with the intermediate accumulation of nitrite. Electron recoveries were between 90 and 100 %, taking assimilated E2 into account. With nitrate as the electron acceptor, the bacterium also grew on fatty acids (C(2) to C(6)), isobutyrate, crotonate, dl-lactate, pyruvate, fumarate and succinate. Phylogenetic analysis of its 16S rRNA gene sequence revealed that strain AcBE2-1(T) represents a separate line of descent within the family Rhodocyclaceae (Betaproteobacteria). The closest relatives are the cholesterol-degrading, denitrifying bacteria Sterolibacterium denitrificans DSM 13999(T) and strain 72Chol (=DSM 12783), with <93.9 % sequence similarity. The G+C content of the DNA was 61.4 mol%. Detection of a quinone system with ubiquinone Q-8 as the predominant compound and a fatty acid profile that included high concentrations of C(16 : 1)omega7c/iso-C(15 : 0) 2-OH and C(16 : 0), in addition to C(18 : 1)omega7c and small amounts of C(8 : 0) 3-OH, supported the results of the phylogenetic analysis. On the basis of 16S rRNA gene sequence data in combination with chemotaxonomic and physiological data, strain AcBE2-1(T) (=DSM 16959(T)=JCM 12830(T)) is placed in a new genus Denitratisoma gen. nov. as the type strain of the type species Denitratisoma oestradiolicum gen. nov., sp. nov.
Fahrbach
Michael
M
Institute of Hygiene and Environmental Medicine, RWTH Aachen, Pauwelsstr. 30, D-52074 Aachen, Germany.
Kuever
Jan
J
Bremen Institute for Materials Testing, Foundation Institute for Materials Science, Paul-Feller-Str. 1, D-28199 Bremen, Germany.
Meinke
Ruth
R
Bremen Institute for Materials Testing, Foundation Institute for Materials Science, Paul-Feller-Str. 1, D-28199 Bremen, Germany.
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32 (IFZ), D-35392 Giessen, Germany.
Hollender
Juliane
J
Eawag, Swiss Federal Institute of Aquatic Science and Technology, CH-8600 Dübendorf, Switzerland.|Institute of Hygiene and Environmental Medicine, RWTH Aachen, Pauwelsstr. 30, D-52074 Aachen, Germany.
D001482:Base Composition; D020562:Betaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002374:Catalase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004958:Estradiol / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009068:Movement; D009584:Nitrogen / Q000378:metabolism*; D010088:Oxidoreductases / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000737:chemistry; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013171:Spores, Bacterial; D014867:Water / Q000378:metabolism; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014867:Water; D002245:Carbon Dioxide; D004958:Estradiol; D010088:Oxidoreductases; D002374:Catalase; D009584:Nitrogen