[Development of radioimmunoassay for human sperm protein 17].
OBJECTIVE
To develop a method of radioimmunoassay for human sperm protein 17(Sp17) determination.


METHODS
Anti-recombinant human Sp17 antibody was prepared, the labeling of 125I-rhSp17 performed by chloramine T method, and radioimmunoassay of Sp17 developed.


RESULTS
The assay range was 3.3 to approximately 800 microg/L, the sensitivity was 2.0 microg/L, and the intra- and inter-assay coefficients of variation (CV) were 7.5% to approximately 9.8% and 8.2% to approximately 13.2%, respectively. The serum Sp17 level in normal subjects was (15.60 +/- 7.66) microg/L (n = 59).


CONCLUSIONS
This radioimmunoassay of Sp17 fulfills the reasonable requirements of clinical routine and scientific studies in terms of specificity, sensitivity and practicability. Measurement of Sp17 concentration is useful for assessing its native distribution and aberrant expression.
Wang
Guo-hong
GH
Center of Medical Laboratory Sciences of PLA, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
Li
Fang-qiu
FQ
Sun
Wei
W
Xu
Rui-ji
RJ
Zhang
Zhong-shu
ZS
Wang
Xiao-jie
XJ
D000818:Animals; D000954:Antigens, Surface / Q000097:blood* / Q000276:immunology; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D008565:Membrane Proteins; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000954:Antigens, Surface; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C090172:SPA17 protein, human