Use of differentiating embryonic stem cells in pharmacological studies.
Adipocytes and osteoblasts are derived from a common precursor cell. It has been proposed that the bone loss commonly seen during aging or in the pathology of osteoporosis might be partly caused by a deregulation of the normal balance between osteoblast and adipocyte differentiation. In vitro differentiation of mouse embryonic stem cells toward the adipogenic and the osteogenic lineages provides a powerful system for testing effects of compounds on the developmental switch between adipogenesis and osteogenesis and identification of pharmacological targets. In this chapter, an improved protocol to commit mouse embryonic stem cells into both lineages at a correct rate is detailed.
Wdziekonski
Brigitte
B
Cellules Souches et Différenciation, Centre de Biochimie, Institut de Recherches Signalisation Biologie du Développement et Cancer, Nice, France.
Villageois
Phi
P
Vernochet
Cécile
C
Phillips
Blaine
B
Dani
Christian
C
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D017931:DNA Primers / Q000235:genetics; D004353:Drug Evaluation, Preclinical; D004622:Embryo, Mammalian / Q000166:cytology*; D015870:Gene Expression; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D012313:RNA