[Tryptase activity in colon mucosal samples of children with inflammatory bowel disease].
BACKGROUND
mast cells are dispersed in many tissues, especially in the digestive and respiratory system mucosal membranes. Tryptase is the most important proteinase released from mast cells after degranulation. It influences strongly the cells and tissues by activating the inflammatory process.


OBJECTIVE
was to assess the activity of tryptase in colon mucosa samples in children with inflammatory bowel diseases (IBD) and in children with bleedings from lower part of gastrointestinal tract (GTB), without inflammation.


METHODS
a group of 30 children with IBD was analyzed in the study. IBD is formed by three disease entities: ulcerative colitis (UC) - 14 patients, Crohn's disease (CD) - 9 patients and non-specific colitis (NSC) - 7 patients. Moreover, a group of 18 children with bleeding from lower part of gastrointestinal tract was studied. The activity of tryptase in homogenates of colon mucosal samples was estimated fluoroimmunoenzymatically.


RESULTS
the results of our analysis showed no statistically important difference between the mean activity of tryptase in groups of children with IBD and GTB (31442 +/- 1304 vs 31868 +/- 775 ug/l). The study of tryptase activities in different disease entities of IBD group showed, that its value in ulcerative colitis group was 31382 +/- 1170 ug/l, in Crohn's disease group it was 31536 +/- 1120 ug/l; in non-specific colitis group the tryptase activity was 32277 +/- 498 ug/l. The analysis with Kruskal-Wallis Anova test revealed that the differences are statistically significant (p = 0.034). In post hoc test the outstanding value is the tryptase activity in children with NSC. Activity of tryptase in colon in much higher than its activity in plasma (normal range 1-19 ug/l).


CONCLUSIONS
the activity of tryptase in mucosal membrane samples is much higher than in blood. The extent of mast cells degranulation may be dependent on the form of IBD.
Popadiuk
Stefan
S
Klinika Pediatrii, Gastroenterologii i Onkologii Dzieciecej, Akademia Medyczna, ul. Nowe Ogrody 1-6, 80-803 Gdansk, Poland. step@amg.gda.pl
Renke
Joanna
J
Gleń
Jolanta
J
Landowski
Piotr
P
Kamińska
Barbara
B
Szlagatys-Sidorkiewicz
Agnieszka
A
Szumera
Małgorzata
M
Ulko
Piotr
P
Korzon
Maria
M
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003093:Colitis, Ulcerative / Q000201:enzymology; D003106:Colon / Q000201:enzymology* / Q000473:pathology; D003424:Crohn Disease / Q000201:enzymology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000201:enzymology*; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000473:pathology; D008297:Male; D053802:Tryptases / Q000032:analysis*
D053802:Tryptases