title: Urine flow cytometer quantification of leukocytes in samples containing a large proportion of lymphocytes.
abstract: OBJECTIVE
  We compared leukocyte counts obtained by cytometric analysis and Fuchs-Rosenthal (FR) chamber counting in different proportions of lymphocytes (Lym%) suspensions and cerebrospinal fluid (CSF).
  
  
  METHODS
  UF-100 (UF) was evaluated. For preparation of cell suspensions, gradient density centrifugation method was used.
  
  
  RESULTS
  The regression equation for UF and FR chamber counting of the cell suspensions was y=0.88x+18.8 WBC/microL (r=0.832, n=106). For a few high Lym% samples, markedly underestimated WBC counts were obtained by UF.
  
  
  CONCLUSIONS
  Underestimated WBC count is due not to systematic error but to random error. Counts of the "other" population by UF may be useful for detection of underestimated samples.
authors.affiliation: Laboratory for Clinical Investigation, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka 565-0871, Japan. yamaha@hp-lab.med.osaka-u.ac.jp
authors.forename: Hachiro
authors.initials: H
authors.lastname: Yamanishi
authors.forename: Nobuko
authors.initials: N
authors.lastname: Imai
authors.forename: Toshinari
authors.initials: T
authors.lastname: Ohmine
authors.forename: Miho
authors.initials: M
authors.lastname: Nishiyama
authors.forename: Etsuji
authors.initials: E
authors.lastname: Suehisa
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Kanakura
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Iwatani
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology*