title: Effects of cinnamic acid on expression of tissue factor induced by TNFalpha in endothelial cells and its mechanisms.
abstract: BACKGROUND
  To observe the effects of cinnamic acid (CINN) on the expression of tissue factor (TF) induced by tumor necrosis factor alpha (TNFalpha) in human umbilical vein endothelium-derived cell line (ECV304) and the related mechanisms.
  
  
  METHODS
  Cellular TF activity was determined with 1-stage clotting assay in the presence or absence of anti-TF antibody or DEGR-Vlla. TF mRNA was examined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). I-kappaB was measured by Western blot analysis. Immunohistochemical analysis was performed to evaluate the activation of NF-kappaB.
  
  
  RESULTS
  CINN (500 microg/mL) significantly decreased the TF activity in ECV304 induced by TNFalpha (1000 U/mL) (p < 0.01) in a concentration-dependent fashion (r = -0.953, p < 0.05). CINN also inhibited the expression of TF mRNA induced by TNFalpha. Immunohistochemical analysis demonstrated NF-kappaB translocation from the cytoplasm to the nucleus, and Western blot analysis showed I-KB decrement in cytoplasm after treatment of endothelial cells with TNFalpha. CINN diminished these changes induced by TNFalpha.
  
  
  CONCLUSIONS
  CINN is able to attenuate TNFa-induced TF expression of vascular endothelial cells by inhibiting NF-kappaB activation.
authors.affiliation: Hemostasis Physiology Laboratory, Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, PR China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Li
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wen
authors.forename: Xiaofan
authors.initials: X
authors.lastname: He
authors.forename: Shilin
authors.initials: S
authors.lastname: He
mesh_terms: D002478:Cells, Cultured; D002934:Cinnamates / Q000494:pharmacology*; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D020672:I-kappa B Proteins / Q000032:analysis; D016328:NF-kappa B / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D013925:Thromboplastin / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D002934:Cinnamates; D020672:I-kappa B Proteins; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C029010:cinnamic acid; D013925:Thromboplastin