Heterogeneity of capillary endothelial cells for basic fibroblast growth factor-induced paracrine signaling.
In this study, the authors isolated morphologically different capillary endothelial cells, designated as BCE-1 and BCE-2 cells, from bovine adrenal cortex. By a series of experiments involving proliferation, migration, and tubular-like structure formation assays, the authors found that the two BCE clones showed a clearly different response to basic fibroblast growth factor (bFGF). Similar to these results, the ERK-1/2 in the BCE-1 cells was phosphorylated by bFGF or vascular endothelial growth factor (VEGF), whereas that of the BCE-2 cells was phosphorylated only by VEGF. However, when the BCE-2 cells were transfected with FGF receptor 1 cDNA, the ERK-1/2 of these cells was phosphorylated by exogenous bFGF. Receptor binding experiments revealed that BCE-2 cells expressed high-affinity tyrosine-kinase FGF receptors approximately twofold less than BCE-1 cells. Transfection and receptor binding studies suggest a possibility that the poor response of the BCE-2 cells to exogenous bFGF is derived from the limitation of functional availability of high affinity FGF receptors. On the other hand, when both BCE clones were treated with anti-bFGF antibodies, basal formation of tubular-like structure in both clones were strongly inhibited, indicating that endogenous bFGF plays a role in in vitro angiogenesis of both BCE clones. Taken together, these data show that the isolated capillary endothelial cells are heterogeneous for paracrine but not autocrine bFGF signaling, and suggest that the diversity of capillary endothelial cells can occur by angiogenic factors, such as bFGF.
Lee
Tae-Hee
TH
Laboratory of Functional Genomics, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
Lee
Hyung-Chahn
HC
Son
Young Sook
YS
Kang
Mi-Ae
MA
Park
Myung-Jin
MJ
Nam
Myeong-Jin
MJ
Lee
Seung-Hoon
SH
Hong
Seok-Il
SI
D000302:Adrenal Cortex / Q000098:blood supply; D000818:Animals; D002196:Capillaries / Q000166:cytology; D002417:Cattle; D018929:Cell Culture Techniques; D002469:Cell Separation; D048430:Cell Shape; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D019899:Paracrine Communication; D010766:Phosphorylation; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D048049:Extracellular Signal-Regulated MAP Kinases