Dendrimer-based nanoprobe for dual modality magnetic resonance and fluorescence imaging.
A novel PAMAM dendrimer-based nanoprobe for dual magnetic resonance and fluorescence imaging modalities was synthesized. Fluorescence studies revealed that Gd(III) complexation to the probe has no effect on the quantum yield; however, increases in the dye content resulted in partial quenching. The potential of the new nanoprobe, G6-(Cy5.5)(1.25)(1B4M-Gd)(145), as a dual modality imaging agent was demonstrated in vivo by the efficient visualization of sentinel lymph nodes in mice by both MRI and fluorescence imaging modalities.
Talanov
Vladimir S
VS
Radiation Oncology Branch, Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1002, USA. martinwb@mail.nih.gov
Regino
Celeste A S
CA
Kobayashi
Hisataka
H
Bernardo
Marcelino
M
Choyke
Peter L
PL
Brechbiel
Martin W
MW
D000818:Animals; D050091:Dendrimers / Q000737:chemistry*; D005453:Fluorescence; D005682:Gadolinium / Q000737:chemistry*; D008198:Lymph Nodes / Q000473:pathology; D046911:Macromolecular Substances / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D051379:Mice; D049329:Nanostructures; D014054:Tomography / Q000295:instrumentation / Q000379:methods*
D050091:Dendrimers; D046911:Macromolecular Substances; D005682:Gadolinium