[Expression analysis of different collagens and cytokines in cartilage cells derived from arthrotic hip and knee joints].
OBJECTIVE
Osteoarthritis (OA) is characterized by an irreversible destruction of articular cartilage. This is associated with a multiplicity of factors, causing an increased catabolic metabolism in cartilage. However, the prevalence of the OA is very variable in different joints. Therefore , we conducted a comparative analysis of chondrocytes derived from knee and hip joints with respect to their expression of inflammatory factors, such as IL-1beta, IL-1beta-receptorantagonist, iNOS, components of cartilage matrix (collagen I, II, and VI) as well as vimentin.


METHODS
Different cytokines and proteins were detected by immune-histochemical staining of cartilage samples ex vivo. Further, chondrocytes were isolated from OA knee and hip joints, expanded in vitro and gene expression patterns were investigated by quantitative RT-PCR.


RESULTS
Chondrocytes from knee and hip joints of OA patients express collagenes I, II and VI, IL-1beta and IL-1beta-RA, iNOS as well as Vimentin. A significant difference in gene expression patterns was not found in chondrocytes from the hip joints versus the knee joint ex vivo or in primary culture cells in vitro. However, in vitro the expression of type I collagen exceeded the expression of type II collagen. The IL-1beta-expression was high ex vivo, remained low during primary culture but was significantly elevated after primary culture in hip chondrocytes.


CONCLUSIONS
Osteoarthritic gene expression patterns in cells derived from hip or knee joints ex vivo and in primary culture were not significantly different. We conclude that the rather frequent occurrence of OA in these joints in comparison to the ankle joint may be associated with a close physiological relation of cells in these joints. However, future studies which will include ankle cartilage must be investigated in further detail.
Hoberg
M
M
Klinik für Orthopädie, Universitätsklinikum Tübingen. Maik.Hoberg@med.uni-tuebingen.de
Aicher
W K
WK
Wülker
N
N
Rudert
M
M
D000368:Aged; D015415:Biomarkers / Q000032:analysis; D002358:Cartilage, Articular / Q000276:immunology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000276:immunology*; D003094:Collagen / Q000276:immunology*; D005260:Female; D015870:Gene Expression / Q000276:immunology; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D015207:Osteoarthritis, Hip / Q000276:immunology*; D020370:Osteoarthritis, Knee / Q000276:immunology*
D015415:Biomarkers; D003094:Collagen