Isolation and characterization of novel marine Roseobacter clade members producing unique intracellular chromium-rich aggregates.
The marine Roseobacter clade comprises one of the largest fractions of heterotrophic marine bacteria and accounts for about 16% of 16S rRNA gene clones retrieved from marine bacterioplankton. Their global distribution seems to be related to oceanic water masses and their environmental and biogeochemical properties. In this study, we report isolation and characterization of novel Roseobacter clade members from the Yellow Sea, China. Phylogenetic analysis of 16S rRNA gene sequences reveals that the new isolates (YSCB1, YSCB2, YSCB3 and YSCB4) are closely related to uncultured Arctic seawater bacterium R7967 (99.57-100% sequence identity) and to the cultured Roseobacter sp. DSS-1 (99.27-99.76% sequence identity) isolated from the southeastern coastal water of the USA. Interestingly, YSCB strains possess unique intracellular chromium-containing aggregates. Therefore, these novel Roseobacter clade members exhibit a peculiar property in mineral biogeneration.
Gao
Jun
J
Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Nahai road 7, Qingdao 266071, China.
Pan
Hongmiao
H
Xiao
Tian
T
Barbier
Georges
G
Wang
Zifeng
Z
Yue
Haidong
H
Sun
Song
S
Nitsche
Serge
S
Bernadac
Alain
A
Pradel
Nathalie
N
Wu
Long-Fei
LF
D002857:Chromium / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041904:Roseobacter / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002857:Chromium