title: Purification, crystallization and preliminary X-ray diffraction studies of Rab11 in complex with Rab11-FIP2.
abstract: The small GTPase Rab11 regulates the recycling of endosomes back to the plasma membrane. In its active GTP-bound form, Rab11 binds a novel set of effectors termed the Rab11 family of interacting proteins (Rab11-FIPs) which contain a conserved C-terminal Rab-binding domain (RBD) of unknown structure. Here, a complex of Rab11 with the RBD of Rab11-FIP2 has been purified and crystallized in the trigonal space group P3(1)21, with unit-cell parameters a = 64.99, b = 64.99, c = 112.59 angstroms. Static light-scattering analyses of the molecular weight of the complex in solution are consistent with two copies of Rab11 and two copies of Rab11-FIP2 in the complex.
authors.affiliation: School of Biochemistry and Immunology, Trinity College, Dublin 2, Ireland.
authors.forename: William N
authors.initials: WN
authors.lastname: Jagoe
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Jackson
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Lindsay
authors.forename: Mary W
authors.initials: MW
authors.lastname: McCaffrey
authors.forename: Amir R
authors.initials: AR
authors.lastname: Khan
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003460:Crystallization; D008027:Light; D011487:Protein Conformation; D012542:Scattering, Radiation; D014961:X-Ray Diffraction; D020691:rab GTP-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D002352:Carrier Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins