title: Structural and inhibitory properties of a plant proteinase inhibitor containing the RGD motif.
abstract: Purified from Bauhinia rufa seeds, BrTI is a Kunitz proteinase inhibitor that contains the RGD sequence. BrTI inhibits trypsin (K(iapp) 2.9 nM) and human plasma kallikrein (K(iapp) 14.0 nM) but not other related enzymes. The synthetic peptide YLEPVARGDGGLA-NH(2) (70 microM) inhibited the adhesion to fibronectin of B16F10 (high-metastatic B16 murine mouse melanoma cell line) and of Tm5 (murine melanoma cell lines derived from a non-tumorigenic lineage of pigmented murine melanocytes, melan-a). YLEPVARGEGGLA-NH(2) in which Asp(9) was changed into Glu does not affect the cell attachment. Moreover, this peptide was functional only when the sequence present in the native protein was preserved, since YLIPVARGDGGLA-NH(2) in which Glu(3) was changed into Ile does not interfere with B16F10 and was less effective on Tm5 cell line adhesion. Neither YLEPVARGDGGLA-NH(2), YLIPVARGDGGLA-NH(2) or YLEPVARGEGGLA-NH(2) inhibit the interaction of RAEC (endothelial cell line from rabbit aorta) with fibronectin.
authors.affiliation: Department of Biochemistry, Universidade Federal de São Paulo-Escola Paulista de Medicina, Rua Três de Maio 100, 04044-020 São Paulo, SP, Brazil.
authors.forename: Adriana M
authors.initials: AM
authors.lastname: Nakahata
authors.forename: Norlene R
authors.initials: NR
authors.lastname: Bueno
authors.forename: Hugo A O
authors.initials: HA
authors.lastname: Rocha
authors.forename: Célia R C
authors.initials: CR
authors.lastname: Franco
authors.forename: Roger
authors.initials: R
authors.lastname: Chammas
authors.forename: Clovis R
authors.initials: CR
authors.lastname: Nakaie
authors.forename: Miriam G
authors.initials: MG
authors.lastname: Jasiulionis
authors.forename: Helena B
authors.initials: HB
authors.lastname: Nader
authors.forename: Lucimeire A
authors.initials: LA
authors.lastname: Santana
authors.forename: Misako U
authors.initials: MU
authors.lastname: Sampaio
authors.forename: Maria Luiza V
authors.initials: ML
authors.lastname: Oliva
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D036481:Bauhinia / Q000737:chemistry* / Q000235:genetics; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007610:Kallikreins / Q000037:antagonists & inhibitors; D008546:Melanoma, Experimental / Q000188:drug therapy; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D011817:Rabbits; D017386:Sequence Homology, Amino Acid
chemical_list: C053865:Kunitz-type protease inhibitor, plant; D009842:Oligopeptides; D010455:Peptides; D010940:Plant Proteins; C047981:arginyl-glycyl-aspartic acid; D007610:Kallikreins