Retention behaviour of beta-blockers in HPLC using a monolithic column.
The chromatographic behaviour of a new HPLC-stationary phase (Chromolith RP-18e) is described for separation of beta-blockers. The effects of the different chromatographic conditions (buffer system, pH value, content of organic modifier, injection volume and flow rate) on the separation behaviour were studied. At higher flow rates the peaks seemed to be more symmetrical than at lower flow rates. The use of buffer or salt in the mobile phase for this separation is found to be very essential and the counter-anion type of this buffer or salt significantly affected the retention behaviour of beta-blockers while the cation type did not play the same important role.
Hashem
Hisham
H
Institute of Pharmacy, Pharmaceutical/Medicinal Chemistry, Ernst-Moritz-Arndt-University Greifswald, Greifswald, Germany.
Jira
Thomas
T
D000319:Adrenergic beta-Antagonists / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012492:Salts / Q000737:chemistry
D000319:Adrenergic beta-Antagonists; D011108:Polymers; D012492:Salts