The detection of human sapoviruses with universal and genogroup-specific primers.
Sapovirus (SV) causes gastroenteritis in humans and comprises genetically divergent viruses. A nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the capsid-protein-coding region was developed using universal and genogroup-specific primer sets. The universal primers were capable of detecting human SV genogroups I, II, IV and V. Genetic analysis of the amplified products enabled us to phylogenetically determine the genotypes of the viruses. In addition, genogroup-specific primers that amplified different lengths of the amplicon depending on the genogroup were developed. These genogroup-specific primers were also used as inner primers for the nested PCR. These two simple RT-PCR methods are powerful tools for both detection and epidemiological studies of human SV.
Okada
M
M
Division of Virology, Chiba Prefectural Institute of Public Health, Chuo-ku, Chiba-shi, Chiba, Japan. m.okd4@mc.pref.chiba.lg.jp
Yamashita
Y
Y
Oseto
M
M
Shinozaki
K
K
D017250:Caliciviridae Infections / Q000175:diagnosis; D017931:DNA Primers; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000175:diagnosis / Q000821:virology; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029341:Sapovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers