title: Speeding up three-dimensional protein NMR experiments to a few minutes.
abstract: We demonstrate for different protein samples that three-dimensional HNCO and HNCA correlation spectra may be recorded in a few minutes acquisition time using the band-selective excitation short-transient sequences presented here. This opens new perspectives for the NMR structural investigation of unstable protein samples and real-time site-resolved studies of protein kinetics.
authors.affiliation: Institut de Biologie Structurale Jean-Pierre Ebel CNRS-CEA-UJF, 41 rue Jules Horowitz, 38027 Grenoble, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Schanda
authors.forename: Hélène
authors.initials: H
authors.lastname: Van Melckebeke
authors.forename: Bernhard
authors.initials: B
authors.lastname: Brutscher
mesh_terms: D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D013997:Time Factors