title: Analysis of single nucleotide polymorphism sites in exon 4 of the p53 gene using high-performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry.
abstract: Three groups of four oligonucleotides with special single nucleotide polymorphisms (SNP) sites in exon 4 of the p53 gene were analyzed with ion-pair reversed-phase high-performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry. The retention order of four oligonucleotides with SNPs was C < G < A < T, regardless of whether the polymorphisms were at the 3' end, the 5' end, or the middle of the oligonucleotides. The charge state of the molecular ion affects the MS/MS spectra of the oligonucleotides. SNPs at the 3' end can be easily identified from the fragmentation pattern of the 2- charge state, but not from the 3- charge state, especially from the w1 fragment. The single base may be taken as the symbol of the 5' end SNP site derived from [M3H]2, but not from the [M3H]2 charge state. The oligonucleotides with SNPs in the middle were also determined from the [M2H]2 precursor ion.
authors.affiliation: State Key Laboratory of Organic Geochemistry, Guangzhou Research Center of Mass Spectrometry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Renfang
authors.initials: R
authors.lastname: Song
authors.forename: Wenbing
authors.initials: W
authors.lastname: Zhang
authors.forename: Huayong
authors.initials: H
authors.lastname: Chen
authors.forename: Humin
authors.initials: H
authors.lastname: Ma
authors.forename: Yulian
authors.initials: Y
authors.lastname: Dong
authors.forename: Guoying
authors.initials: G
authors.lastname: Shen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005091:Exons / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics*; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D020641:Polymorphism, Single Nucleotide; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009838:Oligodeoxyribonucleotides