[Construction of a recombinant adenoviral vector carrying integrin-alpha v and the influence of integrin-alpha on the adhesion characteristics of SMMC-7721 cells].
OBJECTIVE
To establish human integrin-alpha v adenovirus expressed in human liver cancer SMMC-7721 cells and analyze the characteristics of integrin-alpha adhesion to liver cancer cells.


METHODS
Human integrin-alpha v cDNA was cloned into the plasmid pAdTrack-CMV. Then, pAdTrack-integrin-alpha v was cotransformed with adenoviral backbone vector into E. coli strain BJ5183. The recombinant adenoviral plasmid was identified by restriction analysis with Pac I and transfected into HEK-293A cells to package and amplify recombinant adenovirus particles. Western blot showed that integrin-alpha v gene was exactly transcript and expressed in SMMC-7721 cells. Twenty four hours after transfection, the effect of integrin-alpha on adhesion of SMMC-7721 cells was detected by adhesion experiment.


RESULTS
Recombinant adenovirus vector of integrin-alpha v gene was successfully constructed and high titers of recombinant adenovirus was obtained. Adhesion cell count was significantly higher in integrin-alpha v transfected cells than in untransfected and Ad-null-transfected cells (P<0.01).


CONCLUSIONS
Over expression of integrin-alpha v promotes adhesion of SMMC-7721 cells, is an important molecular mechanism of tumor metastasis.
Li
Xu-yan
XY
Department of Pathophysiology, Key Laboratory of Functional Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, Guangdong, China.
Liu
Jing-hua
JH
Li
Zhi-jie
ZJ
Mo
Yong-yan
YY
Liu
Ya-wei
YW
Liu
Zhi-feng
ZF
Deng
Peng
P
Jiang
Yong
Y
D000256:Adenoviridae / Q000235:genetics; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D039564:Integrin alphaV / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D010957:Plasmids / Q000235:genetics; D014162:Transfection
D018076:DNA, Complementary; D039564:Integrin alphaV