title: Biological fingerprinting analysis by liquid chromatography/mass spectrometry for evaluation of DNA structural selectivity of multiple compounds in natural products.
abstract: A method by combination of centrifugal ultrafiltration (CUF) sampling with liquid chromatography-mass spectrometry (LC-MS) analysis was established to evaluate the DNA structure and sequence selectivity of the multiple compounds in a small molecule library. The developed method was applied to analyze the extracts of natural products Coptis chinensis Franch and Rheum palmatum (L.). From the obtained biological fingerprinting chromatograms, 7 compounds in C. chinensis Franch and 11 in R. palmatum (L.) were screened out as DNA binding agents. Most of these compounds were identified by standards and LC-MS analysis after the sample pretreatment with the DNA immobilized cartridge. DNA structural binding preference of the multiple active compounds in these two extracts was then evaluated simultaneously without purification.
authors.affiliation: National Chromatographic R. & A. Centre, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xingye
authors.initials: X
authors.lastname: Su
authors.forename: Liang
authors.initials: L
authors.lastname: Kong
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Xueguo
authors.initials: X
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Guo
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D001665:Binding Sites; D002853:Chromatography, Liquid / Q000379:methods; D020650:Combinatorial Chemistry Techniques; D039323:Coptis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D016172:DNA Fingerprinting; D013058:Mass Spectrometry / Q000379:methods; D008954:Models, Biological; D012250:Rheum / Q000737:chemistry*
chemical_list: D004247:DNA