Serological and molecular ecology of Listeria monocytogenes isolates collected from 13 French pork meat salting-curing plants and their products.
The purpose of this study was dual: 1. to evaluate the serotype distribution of 1028 Listeria monocytogenes isolates collected in 13 French salting factories and their products and 2. to identify sources of L. monocytogenes contamination in these factories and trace the routes of spread by PFGE (Pulsed-Field Gel Electrophoresis) typing. Serotypes 1/2a, 1/2b, 1/2c, 4b and 4e occurred. Pulsotype diversity was high among strains collected in plants and products. Furthermore, strains showing similar pulsotypes occurred on the same surfaces after an interval of at least two weeks and in unrelated factories. Forty five strains were genetically closely related to a 4b serotype L. monocytogenes strain isolated from a human clinical case of listeriosis. Our results highlighted the fact that L. monocytogenes is introduced into meat processing plants through raw meat. To overcome such contamination, suppliers of raw material should adhere to specific microbiological control measures. In addition, more attention should be focused on the appropriateness and compliance with procedures of cleaning and disinfection.
Thévenot
D
D
Unité de Microbiologie Alimentaire et Prévisionnelle, Ecole Nationale Vétérinaire de Lyon, B.P. 83, 69280 Marcy l'étoile, France. d.thevenot@vet-lyon.fr
Delignette-Muller
M-L
ML
Christieans
S
S
Leroy
S
S
Kodjo
A
A
Vernozy-Rozand
C
C
D000818:Animals; D003257:Consumer Product Safety; D016521:Electrophoresis, Gel, Pulsed-Field; D005506:Food Contamination / Q000032:analysis* / Q000517:prevention & control; D005511:Food Handling / Q000379:methods / Q000592:standards*; D005516:Food Microbiology; D005525:Food-Processing Industry / Q000592:standards*; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification / Q000302:isolation & purification*; D008461:Meat Products / Q000382:microbiology*; D010802:Phylogeny; D012703:Serotyping; D013552:Swine