Applicability of the modified universal calibration of gel permeation chromatography on proteins.
The modified universal calibration of gel permeation chromatography (GPC) has been applied in the case of native proteins. Plotting log([eta]M/Phi) versus elution volume, instead of log[eta]M versus elution volume used till now, we obtain unique curves with different proteins and non-proteonic polymers ([eta]: intrinsic viscosity, M: molecular mass, Phi: Flory's parameter). The values of Flory's parameter Phi are calculated for each protein using an indirect method based on GPC.
Dondos
Anastasios
A
Department of Chemical Engineering, University of Patras, 26504 Patras, Greece. dondos@chemeng.upatras.gr
D002138:Calibration; D002850:Chromatography, Gel / Q000379:methods*; D008433:Mathematics; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D014783:Viscosity
D011506:Proteins