Constituents from the fruiting bodies of Ganoderma applanatum and their aldose reductase inhibitory activity.
Eight compounds were isolated from the fruiting bodies of Ganoderma applanatum, and were identified as 2-methoxyfatty acids (1), 5-dihydroergosterol (2), ergosterol peroxide (3) 3beta,7beta, 20,23xi-tetrahydroxy-11,15-dioxolanosta-8-en-26-oic acid (4), 7beta,20,23xi-trihydroxy-3,11,15-trioxolanosta-8-en-26-oic acid (5), cerevisterol (6), 7beta,23xi-dihydroxy-3,11,15-trioxolanosta-8,20E (22)-dien-26-oic acid (7), and 7beta-hydroxy-3,11,15,23-tetraoxolanosta-8,20E(22)-dien-26-oic acid methyl ester (8) by spectral analysis. All compounds were isolated for the first time from this fruiting bodies, and their effect on rat lens aldose reductase (RLAR) activity was tested. Among these eight compounds, ergosterol peroxide (3) was found to exhibit potent RLAR inhibition, its IC50 value being 15.4 microg/mL.
Lee
Sang Hyun
SH
Department of Applied Plant Science, College of Industrial Science, Chung-Ang University, Anseong, Korea.
Shim
Sang Hee
SH
Kim
Ju Sun
JS
Kang
Sam Sik
SS
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D032902:Ganoderma / Q000737:chemistry*; D007908:Lens, Crystalline / Q000187:drug effects / Q000201:enzymology; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley
D004791:Enzyme Inhibitors; D000449:Aldehyde Reductase; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol