title: Study of Staphylococcus aureus adhesion on a novel nanostructured surface by chemiluminometry.
abstract: In recent years the progress in the field of nanotechnologies has offered new possibilities to control the superficial features of implant materials down to a nanoscale level. Several studies have therefore tried to explore the effects of nanostructured biomaterial surfaces on the behavior of eukaryotic cells. However, nanotopography could exert an influence also on the behavior of prokaryotic cells, with relevant implications concerning the susceptibility of implant surfaces to infection. Aim of this study was to examine the behavior of Staphylococcus aureus on polyethylene terephthalate (PET) surfaces either cylindrically nanostructured (PET-N) or flat ion-etched (PET-F), and on tissue culture-grade polystyrene (PS). Microbial adherence was assessed by chemiluminometry under 4 different conditions: (a) bacteria suspended in MEM medium, (b) bacteria in MEM supplemented with 10% fetal bovine serum (FBS), (c) test surfaces preconditioned in FBS, and (d) post-exposure of colonised surfaces to serum-supplemented MEM. Under all circumstances, PET-F and PET-N specimens showed identical bacterial adhesion properties. In the absence of serum, all 3 test materials showed a very high adhesivity to microbial cells and both PET surfaces exhibited greater adhesion than PS. On the contrary, the presence of 10% serum in solution significantly affected cell behavior: the number of microbial cells on all surfaces was drastically reduced, and the adhesion properties of PET surfaces with respect to PS were reversed, with PET being less adhesive. Overall, the specific cylindrical nanostructures created on PET did not significantly influence microbial behavior. Ongoing studies are verifying whether other nanotopographies with different geometry could have more substantial effects.
authors.affiliation: Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna - Italy.
authors.forename: D
authors.initials: D
authors.lastname: Campoccia
authors.forename: L
authors.initials: L
authors.lastname: Montanaro
authors.forename: H
authors.initials: H
authors.lastname: Agheli
authors.forename: D S
authors.initials: DS
authors.lastname: Sutherland
authors.forename: V
authors.initials: V
authors.lastname: Pirini
authors.forename: M E
authors.initials: ME
authors.lastname: Donati
authors.forename: C R
authors.initials: CR
authors.lastname: Arciola
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001672:Biocompatible Materials; D015169:Colony Count, Microbial; D003470:Culture Media; D016895:Culture Media, Serum-Free; D008163:Luminescent Measurements; D008422:Materials Testing; D053758:Nanoparticles; D036103:Nanotechnology; D011093:Polyethylene Terephthalates; D011137:Polystyrenes; D013211:Staphylococcus aureus / Q000502:physiology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D016895:Culture Media, Serum-Free; D011093:Polyethylene Terephthalates; D011137:Polystyrenes