Enhanced in-vitro blood compatibility of 316L stainless steel surfaces by reactive landing of hyaluronan ions.
A novel dry process for immobilization of hyaluronan on stainless steel surfaces is presented. This process that we call reactive landing is based on an interaction of hyperthermal gas-phase hyaluronan ions with plasma-cleaned and activated stainless steel surfaces. Reactive landing is performed on a unique instrument that combines an in-situ plasma reactor with an electrospray ion source and ion transfer optics. Gas-phase hyaluronan anions are obtained by electrospray ionization of sodium hyaluronan solutions and immobilized by reactive landing on large-area stainless steel surfaces. The immobilized hyaluronan withstands extensive washing with polar solvents and solutions, and the washed surfaces maintain the protective properties against blood platelet activation. The mechanism of hyaluronan discharge and immobilization is discussed.
Volný
Michael
M
Department of Chemistry, Bagley Hall, Box 351700, University of Washington, Seattle, Washington 98195-1700, USA.
Elam
W Timothy
WT
Ratner
Buddy D
BD
Turecek
Frantisek
F
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D066298:In Vitro Techniques; D007477:Ions; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D013057:Spectrum Analysis; D013193:Stainless Steel; D013499:Surface Properties; D014965:X-Rays
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D007477:Ions; D013193:Stainless Steel; D006820:Hyaluronic Acid