Transient expression of Drosophila melanogaster deoxynucleoside kinase gene enhances cytotoxicity of nucleoside analogs.
The Drosophila melanogaster deoxynucleoside kinase gene was introduced into HeLa cells with cationic lipids to allow its transient expression, and cytotoxic effects of several nucleoside analogs in the transfected cells were examined. Of the analogs tested, cytotoxicities of 1-beta-D-arabinofuranosylcytosine (araC), 5-fluorodeoxyuridine (FUdR), and 1-(2-deoxy-2-methylene-beta-D-erythro-pentofuranosyl)cytosine (DMDC) were increased by the deoxynucleoside kinase gene. These results suggest that the combination of the transient expression of the Drosophila deoxynucleoside kinase gene and these nucleoside analogs is a candidate for the suicide gene therapy.
Kamiya
Hiroyuki
H
School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo, Japan. hirokam@pharm.hokudai.ac.jp
Ochiai
Hiroshi
H
Harashima
Hideyoshi
H
Ito
Mana
M
Matsuda
Akira
A
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D003561:Cytarabine / Q000378:metabolism / Q000633:toxicity*; D003845:Deoxycytosine Nucleotides / Q000378:metabolism / Q000633:toxicity*; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics; D005467:Floxuridine / Q000378:metabolism / Q000633:toxicity*; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*
C102454:1-(2-deoxy-2-methylene-erthyro-pentofuranosyl)cytosine 5'-triphosphate; D003845:Deoxycytosine Nucleotides; D005467:Floxuridine; D003561:Cytarabine; D017853:Phosphotransferases (Alcohol Group Acceptor); C020018:deoxyribonucleoside kinases