NSET molecular beacon analysis of hammerhead RNA substrate binding and catalysis.
Nanometal surface energy transfer (NSET), which describes an energy transfer event from optically excited organic fluorophores to small metal nanoparticles, may be used as a molecular beacon/ruler similar to FRET, but with advantages over this classical technique. Here we use NSET to measure Mg(2+)-induced conformational changes for a hammerhead ribozyme and confirm these measurements using FRET. These optical experiments enhance our understanding of the different kinetic pathways for this ribozyme.
Jennings
T L
TL
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, 32306-4390, USA.
Schlatterer
J C
JC
Singh
M P
MP
Greenbaum
N L
NL
Strouse
G F
GF
D002384:Catalysis; D004735:Energy Transfer; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D005456:Fluorescent Dyes; D016337:RNA, Catalytic; C427204:hammerhead ribozyme; D006046:Gold; D008274:Magnesium