title: Assessment of cord blood unit characteristics on the day of transplant: comparison with data issued by cord blood banks.
abstract: BACKGROUND
  Selection of a cord blood (CB) unit for allogeneic transplantation relies on graft characterization results provided by cord blood banks (CBBs). The goal was to compare the graft characterization results obtained upon thawing and washing to those provided by CBBs at selection.
  
  
  METHODS
  With tests that assess CB graft characteristics known to impact engraftment, CB units have been analyzed after thaw and before infusion. Our results were compared to data provided by CBBs to determine the impact on engraftment and assess how CBB-supplied information can affect future CB unit selection.
  
  
  RESULTS
  Variability was noted as to the type of information provided by the different CBBs. Also, variability was found between the information provided by CBBs and the graft characterization results obtained upon thawing and washing. In some cases, CB measures known to be predictive of engraftment were found much lower than reported by CBBs. Only the total nucleated cell count, which is the main CB graft selection criterion besides HLA matching, correlated favorably.
  
  
  CONCLUSIONS
  Our data reveal a high degree of variability in graft characteristics provided by CBBs and often poor correlation with results obtained on thawed and washed CB units. We suggest that standardized laboratory procedures aimed at graft characterization should be used by both CBBs and transplant centers to avoid unacceptable discrepancies.
authors.affiliation: Division of Hematology-Oncology, CHU Sainte-Justine, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Eric
authors.initials: E
authors.lastname: Wagner
authors.forename: Michel
authors.initials: M
authors.lastname: Duval
authors.forename: Jean-Hugues
authors.initials: JH
authors.lastname: Dalle
authors.forename: Hugo
authors.initials: H
authors.lastname: Morin
authors.forename: Sonia
authors.initials: S
authors.lastname: Bizier
authors.forename: Josette
authors.initials: J
authors.lastname: Champagne
authors.forename: Martin A
authors.initials: MA
authors.lastname: Champagne
mesh_terms: D000094902:Blood Banking / Q000379:methods*; D001772:Blood Cell Count; D036101:Cord Blood Stem Cell Transplantation / Q000592:standards*; D015925:Cryopreservation; D005312:Fetal Blood; D006085:Graft Survival; D006801:Humans; D011237:Predictive Value of Tests; D011356:Product Labeling; D011786:Quality Control; D012308:Risk Management