Determination of chlorpheniramine and its binding with human serum albumin by capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection.
Tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence (ECL) detection in a capillary electrophoresis separation system was used for the determination of chlorpheniramine (CPM). The experimental conditions, such as the applied potential, separation voltage, injection voltage, injection time and the pH of the separation buffer were considered in detail. The ECL intensity showed two linear responses to CPM, i.e., from 15 microM to 1 mM and from 0.8 microM to 15 microM with a detection limit of 0.5 microM. The binding of CPM with human serum albumin was also monitored using this method and the binding constant was estimated to be 4.1 x 103 M(-1).
Liu
Yingju
Y
Department of Applied Chemistry, College of Sciences, South China Agriculture University, Guangzhou 510642, P.R. China. liuyingju@hotmail.com
Zhou
Wuyi
W
D001665:Binding Sites; D002744:Chlorpheniramine / Q000032:analysis*; D004563:Electrochemistry; D019075:Electrophoresis, Capillary; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods; D009942:Organometallic Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013997:Time Factors
D009942:Organometallic Compounds; D012709:Serum Albumin; C415768:tris(2,2'-bipyridyl)ruthenium(II); D002744:Chlorpheniramine