Expression, purification, crystallization and preliminary crystallographic analysis of the human intracellular chloride channel protein CLIC4.
The human chloride intracellular channel protein CLIC4 has been crystallized by the hanging-drop vapour-diffusion technique using trisodium citrate as the precipitant. The best crystals were obtained by the microseeding method. The crystals diffracted to 2.2 A resolution and were found to belong to space group P121, with unit-cell parameters a = 73.19, b =86.05, c = 73.38 A, beta = 112.99 degrees and three molecule per asymmetric unit.
Li
De-Feng
DF
Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, People's Republic of China.
Li
Yun-Feng
YF
Huang
Qiu-Hua
QH
Zhang
Ying
Y
Wang
Da-Cheng
DC
D018118:Chloride Channels / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
C464459:CLIC4 protein, human; D018118:Chloride Channels; D011994:Recombinant Proteins