title: Genetic instability of BRCA1 gene at locus D17S855 is related to clinicopathological behaviors of gastric cancer from Chinese population.
abstract: OBJECTIVE
  To investigate genetic instability of gene BRCA1 at locus D17S855, and their relationship with clinicopathological characteristics of gastric cancer in Chinese population.
  
  
  METHODS
  Microsatellite instability (MSI) and loss of heterozygosity (LOH) of gene BRCA1 at locus D17S855 were compared between 37 samples of gastric cancer and corresponding non-cancerous gastric tissue.
  
  
  RESULTS
  MSI at locus D17S855 was positive in 7 of 37 samples of gastric cancer (18.95%). MSI had a close relationship with TNM staging but no relation with lymph node metastasis, histological type or tumor differentiation. MSI positive frequency in TNM I + II (31.58%, 6/19) was much higher than that in TNM III + IV (5.56%, 1/18), (P < 0.05). LOH positive rate was 18.92% (7/37). LOH had no relationship to histological type, tumor differentiation or lymph node metastasis, but LOH positive rate in TNM III + IV was 33.33% (6/18), much higher than that in TNM I + II ( 5.26%, 1/19), (P < 0.05). BRCA1 protein was expressed in 14 of 37 samples of gastric cancer. The positive rates of BRCA1 protein in TNM I + II and TNM III + IV were 57.89% and 16.67%, respectively, (P < 0.05). The positive rate of BRCA1 protein was 77.78% in high differentiation samples, 30.77% in middle differentiation and 12.50% in lower differentiation samples, (P < 0.05).
  
  
  CONCLUSIONS
  MSI of BRCA1 gene could be used as a molecular marker in early phases of sporadic gastric cancer in Chinese population. LOH occurs at later period of gastric cancer, therefore, it could be used as prognostic factor.
authors.affiliation: Department of Surgery, Taizhou First People's Hospital, Huangyan 318020, Zhejiang Province, China.
authors.forename: Xue-Rong
authors.initials: XR
authors.lastname: Chen
authors.forename: Wei-Zhong
authors.initials: WZ
authors.lastname: Zhang
authors.forename: Xing-Qiu
authors.initials: XQ
authors.lastname: Lin
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Wang
mesh_terms: D044466:Asian People / Q000235:genetics*; D019313:BRCA1 Protein / Q000235:genetics; D002681:China; D004273:DNA, Neoplasm / Q000235:genetics; D018450:Disease Progression; D019398:Genes, BRCA1; D052138:Genes, Neoplasm / Q000235:genetics; D042822:Genomic Instability; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics; D008207:Lymphatic Metastasis / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D009367:Neoplasm Staging; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000473:pathology*
chemical_list: D019313:BRCA1 Protein; D004273:DNA, Neoplasm