Phenotypic and genetic characterization of vancomycin-resistant enterococci from hospitalized humans and from poultry in Korea.
Vancomycin resistant enterococci (VRE) isolates from humans (23 isolates) and poultry (20 isolates) were characterized by antibiotic susceptibility, vancomycin resistance transferability, pulsed-field gel electrophoresis (PFGE), and structural analysis of Tn1546-like elements. VRE isolates from humans and poultry showed different resistance patterns, transferability, and transfer rate. In addition to these phenotypic differences between humans and poultry VRE, PFGE and the structure of Tn1546-like elements were also distinct. Most poultry isolates (16/20) were identical to the prototype vanA transposon, Tn1546, while most human isolates (21/23) had multiple integrations of insertion sequence. The transmission of VRE and vancomycin resistance determinant between humans and poultry could not be demonstrated in this study.
Jung
Woo Kyung
WK
Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Hong
Soon Keun
SK
Lim
Ji Youn
JY
Lim
Suk Kyung
SK
Kwon
Nam Hoon
NH
Kim
Jun Man
JM
Koo
Hye Cheong
HC
Kim
So Hyun
SH
Seo
Keun Seok
KS
Ike
Yasuyoshi
Y
Tanimoto
Koichi
K
Park
Yong Ho
YH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004251:DNA Transposable Elements; D016521:Electrophoresis, Gel, Pulsed-Field; D016983:Enterococcus / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology* / Q000662:veterinary; D006760:Hospitalization; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010641:Phenotype; D011200:Poultry / Q000382:microbiology*; D011201:Poultry Diseases / Q000382:microbiology; D017422:Sequence Analysis, DNA; D020713:Vancomycin Resistance / Q000235:genetics
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements