Functional redundancy of transcription factor-binding sites in the killer cell Ig-like receptor (KIR) gene promoter.
Variegated expression of inhibitory killer cell Ig-like receptors (KIRs) for MHC class I molecules helps NK cells distinguish normal from aberrant self and avoid autoreactivity. Prior studies of KIR promoters have produced conflicting results and no cis-acting sites have been independently confirmed. We took a comprehensive linker-scanning mutagenesis approach and substituted 24 consecutive 10-bp segments in the human KIR3DL1 promoter. Our analysis revealed eight segments that activated and three segments that repressed KIR transcription. Site-directed mutagenesis and electrophoretic mobility shift assays indicated that optimal KIR transcription requires a proximal Ets site that binds several Ets family members, a cAMP response element (CRE), a Runx site and a site that mediates complex interactions between Ets family members, signal transducer and activator of transcription 5 (STAT5) and YY1; Sp1 also contributes to KIR transcription. KIR transcription was greatly reduced by several compound mutations and was abrogated by a combination of mutations that affected the proximal Ets site, and the CRE, Runx, Sp1 and Ets/STAT sites. The many transcription factors that contribute to KIR transcription are partially redundant in the setting of transient transfection assays, helping to explain why only 0-2 activating sites had been reported in each of three prior studies. We propose that the multiplicity of transcription factors enables NK cells to sustain continuous KIR expression in diverse cellular and cytokine milieus, thus preventing NK autoreactivity.
Presnell
Steven R
SR
Department of Pathology and Laboratory Medicine, University of Kentucky, 800 Rose Street, MS117, Lexington, 40536-0298, USA.
Zhang
Lei
L
Ramilo
Cecilia A
CA
Chan
Huei-Wei
HW
Lutz
Charles T
CT
D001483:Base Sequence; D001665:Binding Sites; D050657:Core Binding Factor alpha Subunits / Q000235:genetics; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D054340:Receptors, KIR; D054341:Receptors, KIR3DL1; D050799:STAT5 Transcription Factor / Q000235:genetics / Q000276:immunology; D016329:Sp1 Transcription Factor / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D050986:YY1 Transcription Factor / Q000235:genetics
D050657:Core Binding Factor alpha Subunits; D017362:Cyclic AMP Response Element-Binding Protein; C515083:KIR3DL1 protein, human; D050783:Proto-Oncogene Proteins c-ets; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054341:Receptors, KIR3DL1; D050799:STAT5 Transcription Factor; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494698:YY1 protein, human