Sustained mitotic block elicits DNA breaks: one-step alteration of ploidy and chromosome integrity in mammalian cells.
Following prolonged mitotic spindle disruption by microtubule poisons, mammalian cells delay their entry into anaphase, then progressively slip out of mitosis and become tetraploid. Normal cells then stop cycling before S-phase onset, but the mechanisms underlying this arrest are still unclear. Here we show that a double block prevents endo-reduplication. First, cells that exit mitosis without a functional microtubule network are driven toward G0. Reconstitution of the network unmasks a second block that relies on DNA double-strand breaks occurring early in the G1 phase that follows the mitotic block. We propose that a stress signal elicited upon mitotic impairment triggers breakage, which couples the leaky spindle checkpoint to the stringent DNA damage response. Consistent with this finding, cells defective for the damage response continue cycling and acquire, within a single cell cycle, both chromosome rearrangements and abnormal chromosome numbers that remarkably mimic the complex genetic hallmark of tumorigenesis.
Quignon
F
F
Institut Curie, Université Pierre et Marie Curie-Paris 6, CNRS UMR 7147, 26 Rue d'Ulm, 75248, Paris Cédex 05, France.
Rozier
L
L
Lachages
A-M
AM
Bieth
A
A
Simili
M
M
Debatisse
M
M
D000230:Adenocarcinoma / Q000473:pathology; D000705:Anaphase; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002478:Cells, Cultured; D002875:Chromosomes; D003110:Colonic Neoplasms / Q000473:pathology; D006224:Cricetinae; D003412:Cricetulus; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage; D005347:Fibroblasts / Q000502:physiology; D016193:G1 Phase / Q000235:genetics; D015321:Gene Rearrangement; D006801:Humans; D015151:Immunoblotting; D017404:In Situ Hybridization, Fluorescence; D008168:Lung / Q000502:physiology; D008870:Microtubules; D008938:Mitosis / Q000187:drug effects / Q000502:physiology*; D008940:Mitotic Index; D015739:Nocodazole / Q000494:pharmacology; D011003:Ploidies; D016192:Resting Phase, Cell Cycle; D008941:Spindle Apparatus
D000970:Antineoplastic Agents; D015739:Nocodazole