title: Prooxidant-antioxidant equilibrium in rowers: effect of a single dose of vitamin E.
abstract: OBJECTIVE
  The purpose of this research was to explain whether a single dose of vitamin E influences on disturbances of pro-oxidant-antioxidant equilibrium induced a laboratory 2,000 m rowing test.
  
  
  METHODS
  The athletes were divided into two groups: vitamin E (1.000 mg alfa-tocopherol acetate) and P (placebo: cod-liver oil) supplemented 3 h before exercise. In the subjects' blood levels of following parameters were estimated: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), lipid peroxidation products (TBARS) and protein carbonyls (PC). Creatine kinase (CK) and beta-glucuronidase (betaG) activities were also measured as markers of muscle damage.
  
  
  RESULTS
  The applied exercise at maximal intensity (lactate: vitamin E=14.39+/-1.77 mmol/L, P=14.09+/-1.4 mmol/L) caused significant changes in the tested parameters in both groups: SOD (vitamin E Ø30%, P Ø50%), CAT (vitamin 70%, P 12%), GPx (vitamin 49%, P 45%), TBARS (vitamin 70%, P 71%), PC (vitamin 41%, P 78%), CK (vitamin 19%, P 32%), betaG (vitamin 42%, P 47%). The P/A ratio (TBARS/SOD+CAT+GPx) was significantly lower in vitamin E in comparison with placebo, but after 30 min and 24 h restitution it returned to pre-exercise level. Between P/A ratio and PC concentration there was observed positive correlation (P<0.001). No effect of vitamin E on CK and betaG activities were observed.
  
  
  CONCLUSIONS
  A single dose of 1,000 mg vitamin E enhances oxidative defense thus attenuates oxidative damage but it is not so much relevant to recommend the antioxidant supplementation by rowers.
authors.affiliation: Department of Medical Sciences, Faculty of Physical Culture, University School of Physical Education of Poznan, Gorzow Wlkp., Poland. agzem@gorzow.home.pl
authors.forename: A
authors.initials: A
authors.lastname: Zembron-Lacny
authors.forename: K
authors.initials: K
authors.lastname: Szyszka
authors.forename: B
authors.initials: B
authors.lastname: Sobanska
authors.forename: R
authors.initials: R
authors.lastname: Pakula
mesh_terms: D000293:Adolescent; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002374:Catalase / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D005966:Glucuronidase / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D010919:Placebos; D011355:Prodrugs / Q000494:pharmacology; D050050:Protein Carbonylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D013177:Sports / Q000502:physiology*; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D010919:Placebos; D011355:Prodrugs; D017382:Reactive Oxygen Species; D014810:Vitamin E; D019344:Lactic Acid; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003402:Creatine Kinase; D005966:Glucuronidase; D024502:alpha-Tocopherol; D024505:Tocopherols