Genetic instability of BRCA1 gene at locus D17S855 is related to clinicopathological behaviors of gastric cancer from Chinese population.
OBJECTIVE
To investigate genetic instability of gene BRCA1 at locus D17S855, and their relationship with clinicopathological characteristics of gastric cancer in Chinese population.


METHODS
Microsatellite instability (MSI) and loss of heterozygosity (LOH) of gene BRCA1 at locus D17S855 were compared between 37 samples of gastric cancer and corresponding non-cancerous gastric tissue.


RESULTS
MSI at locus D17S855 was positive in 7 of 37 samples of gastric cancer (18.95%). MSI had a close relationship with TNM staging but no relation with lymph node metastasis, histological type or tumor differentiation. MSI positive frequency in TNM I + II (31.58%, 6/19) was much higher than that in TNM III + IV (5.56%, 1/18), (P < 0.05). LOH positive rate was 18.92% (7/37). LOH had no relationship to histological type, tumor differentiation or lymph node metastasis, but LOH positive rate in TNM III + IV was 33.33% (6/18), much higher than that in TNM I + II ( 5.26%, 1/19), (P < 0.05). BRCA1 protein was expressed in 14 of 37 samples of gastric cancer. The positive rates of BRCA1 protein in TNM I + II and TNM III + IV were 57.89% and 16.67%, respectively, (P < 0.05). The positive rate of BRCA1 protein was 77.78% in high differentiation samples, 30.77% in middle differentiation and 12.50% in lower differentiation samples, (P < 0.05).


CONCLUSIONS
MSI of BRCA1 gene could be used as a molecular marker in early phases of sporadic gastric cancer in Chinese population. LOH occurs at later period of gastric cancer, therefore, it could be used as prognostic factor.
Chen
Xue-Rong
XR
Department of Surgery, Taizhou First People's Hospital, Huangyan 318020, Zhejiang Province, China.
Zhang
Wei-Zhong
WZ
Lin
Xing-Qiu
XQ
Wang
Jin-Wei
JW
D044466:Asian People / Q000235:genetics*; D019313:BRCA1 Protein / Q000235:genetics; D002681:China; D004273:DNA, Neoplasm / Q000235:genetics; D018450:Disease Progression; D019398:Genes, BRCA1; D052138:Genes, Neoplasm / Q000235:genetics; D042822:Genomic Instability; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics; D008207:Lymphatic Metastasis / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D009367:Neoplasm Staging; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000473:pathology*
D019313:BRCA1 Protein; D004273:DNA, Neoplasm