title: [Expressions of CXCL16/CXCR6 and CXCL12/CXCR4 in first-trimester human trophoblast cells].
abstract: OBJECTIVE
  To investigate the transcription and protein expressions of chemokines CXCL16, CXCL12 and their receptors CXCR6, CXCR4 in first-trimester human cytotrophoblast cells and human choriocarcinoma cell line JAR.
  
  
  METHODS
  Transcriptions of CXCR6, CXCL16, CXCR4, CXCL12 in purified first-trimester human trophoblast cells and JAR line were assessed by semi-quantitative RT-PCR, and protein expressions of CXCR6, CXCL16, CXCR4, CXCL12 were analyzed in primary cultured villous cytotrophoblasts (VCT), extravillous cytotrophoblasts (EVCT), JAR line and placentas by immunostaining.
  
  
  RESULTS
  CXCR6 and CXCR4 were highly transcribed in primary cultured trophoblast cells with mRNA relative level of 1.12 +/- 0.25 and 1.08 +/- 0.11 respectively, and their ligands CXCL16 and CXCL12 were transcribed moderately with mRNA relative level of 0.89 +/- 0.11 and 0.78 +/- 0.10 respectively. It was demonstrated that CXCL16, CXCL12, CXCR6 and CXCR4 were expressed in primary cultured VCT, EVCT, JAR line and placentas by immunostaining.
  
  
  CONCLUSIONS
  The co-expression of CXCL16/CXCR6 and CXCL12/CXCR4 in trophoblast cells may play a role in the proliferation and differentiation of first-trimester trophoblast cells in a manner of autocrine.
authors.affiliation: Institute of Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Da-jin
authors.initials: DJ
authors.lastname: Li
authors.forename: Ming-yan
authors.initials: MY
authors.lastname: Wang
authors.forename: Hai-dong
authors.initials: HD
authors.lastname: Cheng
mesh_terms: D000328:Adult; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000096:biosynthesis / Q000235:genetics; D000075743:Chemokine CXCL16; D019743:Chemokines, CXC / Q000096:biosynthesis / Q000235:genetics*; D002822:Choriocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002824:Chorionic Villi / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D019718:Receptors, CXCR4 / Q000096:biosynthesis / Q000235:genetics; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000235:genetics*; D051116:Receptors, Scavenger / Q000096:biosynthesis / Q000235:genetics; D011991:Receptors, Virus / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: C423727:CXCL16 protein, human; C106330:CXCR6 protein, human; D054377:Chemokine CXCL12; D000075743:Chemokine CXCL16; D019743:Chemokines, CXC; D019718:Receptors, CXCR4; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine; D051116:Receptors, Scavenger; D011991:Receptors, Virus