Induction of cell death in Caco-2 human colon carcinoma cells by ellagic acid rich fractions from muscadine grapes (Vitis rotundifolia).
Possible anticancer mechanisms exerted by polyphenolic compounds contained in fruits and vegetables include antioxidant activity, the inhibition of proliferation, and the induction of apoptosis in cancer cells. This study examined the effects of four isolated polyphenolic extracts from red muscadine grapes (Vitis rotundifolia) on vital cell parameters and the induction of apoptosis in Caco-2 colon carcinoma cells. The magnitude of effects in cell culture was then correlated to polyphenolic composition and antioxidant capacity. Whereas anticancer effects of individual polyphenolic compounds have been demonstrated multiple times, information relating to anticancer effects of polyphenolic extracts is not available in abundance. All four extracts induced apoptosis, decreased cell number, and caused alterations in cell cycle kinetics in a concentration-dependent manner. The efficacy of the polyphenolics on vital cell parameters correlated well to the presence of ellagic acid glycosides and flavonoids and also to the antioxidant capacity. This study demonstrated the anticancer properties of ellagic acid rich extracts from red muscadine juice.
Mertens-Talcott
Susanne U
SU
Department of Pharmaceutics, University of Florida, P. O. Box 110494, Gainesville, Florida 32610-0494, USA.
Lee
Joon-Hee
JH
Percival
Susan S
SS
Talcott
Stephen T
ST
D000975:Antioxidants / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects*; D018938:Caco-2 Cells; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002452:Cell Count; D002453:Cell Cycle; D004610:Ellagic Acid / Q000032:analysis* / Q000494:pharmacology; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D059808:Polyphenols; D017382:Reactive Oxygen Species / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species; D004610:Ellagic Acid; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases