Cell cycle inhibitory protein p27 in human middle ear cholesteatoma.
OBJECTIVE
To evaluate the immunohistochemical and molecular presentation of protein p27 in cholesteatoma.


METHODS
42 cholesteatoma samples and 6 external ear canal skin (EECS) specimens were investigated and analyzed taking into consideration congenital, acquired, recurrent cholesteatoma, and EECS.


RESULTS
The expression of p27 was found in 16 (38.1%) out of 42 specimens of cholesteatoma and in 5 (83.3%) out of 6 specimens of EECS. There was a significant difference in p27-positive staining rate between EECS and cholesteatoma epithelium (p < 0.008). The presence of p27 was detected in 10 cases of acquired cholesteatoma, 2 cases of congenital and 3 cases of recurrent cholesteatoma. There was no significant difference between the presence of p27 in cholesteatoma and EECS (p = 0.01).


CONCLUSIONS
The down-regulation of p27 is a key player in cell cycle control and plays an undefined role in the pathogenesis of all types of cholesteatoma.
Kuczkowski
Jerzy
J
Department of Otolaryngology, Medical University of Gdansk, Gdansk, Poland. jerzyk@amg.gda.pl
Bakowska
Alicja
A
Pawelczyk
Tadeusz
T
Narozny
Waldemar
W
Mikaszewski
Boguslaw
B
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D018424:Cholesteatoma, Middle Ear / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism*; D004424:Ear Canal / Q000378:metabolism / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012008:Recurrence
D050760:Cyclin-Dependent Kinase Inhibitor p27