Development of an enzyme-linked immunosorbent assay for the pyrethroid insecticide cyhalothrin.
A competitive enzyme-linked immunosorbent assay (ELISA) was developed for detection of the pyrethroid insecticide cyhalothrin. Three haptens with an amine or propanoic acid terminus were synthesized and then conjugated with bovine serum albumin to give immunogens. Eight polyclonal antisera produced by rabbits were screened for titers and affinity using three different coating antigens. The antiserum CWB-C had the highest affinity with cyhalothrin and a low affinity with fenvalerate, fenpropathrin, deltamethrin, and fluvalinate. The half-maximum inhibition concentration for cyhalothrin was 37.2 microg/L, and the limit of detection was 4.7 microg/L. The recoveries of different concentrations of cyhalothrin (0.1-2500 microg/L) from fortified tap water, well water, and wastewater samples as determined with the ELISA were 81-114%.
Gao
Hongbin
H
Department of Ecology and Environmental Sciences, College of Resource and Environment and College of Natural Sciences, China Agricultural University, Beijing, 100094, People's Republic of China.
Ling
Yun
Y
Xu
Ting
T
Zhu
Weiwen
W
Jing
Hongyu
H
Sheng
Wei
W
Li
Qing X
QX
Li
Ji
J
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D007306:Insecticides / Q000032:analysis*; D009570:Nitriles / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D014867:Water / Q000032:analysis; D014873:Water Pollutants / Q000032:analysis
D006241:Haptens; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D014873:Water Pollutants; D014867:Water; D012710:Serum Albumin, Bovine; C037304:cyhalothrin