Determination of agmatine in biological samples by capillary electrophoresis with optical fiber light-emitting-diode-induced fluorescence detection.
A capillary electrophoresis (CE)/optical fiber light-emitting diode (LED)-induced fluorescence detection method is developed for the determination of agmatine in biological samples. The agmatine was precolumn-derivatized with fluorescence tagging reagent, fluorescein isothiocyanate (FITC). Optimal separation and determination for agmatine were obtained with an electrophoretic buffer of 20 mM sodium borate (pH 9.2). Under the optimal conditions, the determination of agmatine was achieved in less than 4 min, and the detection limit was 4.1x10(-9) M (S/N = 3). The relative standard deviation (RSD) for 11 parallel determination of agmatine was less than 3.0%. The present CE-LED induced fluorescence detection method has been applied to detect agmatine in rat brain tissue, rat stomach tissue, human serum, and human urine. The level of agmatine in human urine was quantified by CE for the first time and found to be in the range 2.5-4.1x10(-7) M.
Zhao
Shulin
S
College of Chemical Engineering, Sichuan University, Chengdu 610065, China.
Wang
Bing
B
Yuan
Hongyan
H
Xiao
Dan
D
D000376:Agmatine / Q000097:blood / Q000378:metabolism* / Q000652:urine; D000818:Animals; D001921:Brain / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D051381:Rats; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000376:Agmatine