Genotoxic activity of nitroarene-contaminated industrial sludge following large-scale treatment in aerated and non-aerated sacs.
An industrial sludge containing a complex mixture of nitroaromatic compounds was treated in industrial large-scale aerobic and anaerobic biodegradation processes, performed in compost sacs. The goal was to study changes in genotoxicity during the two different oxygen regimes using the umuC genotoxicity assay. The composting sac was actively aerated during 3 months and allowed to mature for another 3 months. The anaerobic sac was not aerated for 5 months and aerated during the last month in order to enhance degradation of remaining organic carbon. The sludge was obtained from the wastewater treatment plant at an industrial area in Karlskoga, Sweden. The biodegradation study was performed at a commercial waste treatment plant in Stockholm, according to the company routine procedure when treating household waste in sealed sacs. The material from the non-aerated system showed increased genotoxicity in the acetone-soluble fraction after treatment, as did the water-soluble fraction. The subsequent aeration period did not decrease the toxicity below the genotoxicity limit. The increase in the water-soluble genotoxic compounds may pose an environmental problem during secondary storage or use of sludge treated this way, since leakage of water-dissolved genotoxic compounds may occur. The composting process also generated genotoxicity, but this was restricted to acetone-soluble compounds, while the water-soluble compounds remained low in genotoxicity. The aerated process therefore seems more favorable in term of risk reduction of this industrial sludge, although it is necessary to optimize the aerated process in order to achieve non-toxic levels of potential genotoxic compounds extractable by organic solvents.
Gustavsson
L
L
Man-Technology-Environment Research Centre (MTM), Department of Natural Sciences, Orebro University, S-70182 Orebro, Sweden. lillemor.gustavsson@karlskogaenergi.se
Engwall
M
M
D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000032:analysis; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D006841:Hydrocarbons, Aromatic / Q000378:metabolism / Q000633:toxicity*; D009152:Mutagenicity Tests; D017672:Nitrogen Compounds / Q000378:metabolism / Q000633:toxicity*; D010100:Oxygen / Q000032:analysis; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013548:Sweden; D014865:Waste Disposal, Fluid / Q000379:methods*; D014866:Waste Products / Q000032:analysis*
D029968:Escherichia coli Proteins; D006841:Hydrocarbons, Aromatic; D017672:Nitrogen Compounds; D014866:Waste Products; D002245:Carbon Dioxide; C078202:UmuC protein, E coli; D004259:DNA-Directed DNA Polymerase; D010100:Oxygen