Sensitive extractive spectrophotometric methods for the determination of trazodone hydrochloride in pharmaceutical formulations.
Two simple, rapid and sensitive extractive spectrophotometric methods have been developed for the assay of trazodone hydrochloride (TRH) in pure and pharmaceutical formulations. These methods are based on the formation of chloroform soluble ion-association complexes of TRH with bromothymol blue (BTB) and with bromocresol purple (BCP) in KCl-HCl buffer of pH 2.0 (for BTB) and in NaOAc-AcOH buffer of pH of 3.6 (for BCP) with absorption maximum at 423 nm and at 408 nm for BTB and BCP, respectively. Reaction conditions were optimized to obtain the maximum color intensity. The absorbance was found to increase linearly with increase in concentration of TRH, which was corroborated by the calculated correlation coefficient values (0.9996, 0.9945). The systems obeyed Beer's law in the range of 0.2-14.5 and 0.2-14.1 microg/ml for BTB and BCP, respectively. Various analytical parameters have been evaluated and the results have been validated by statistical data. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, accurate and suitable for quality control applications.
Sudhir Kumar
Ramanaboyina
R
Department of Chemistry, Karnatak University, Dharwad-580 003, India.
Manjunatha
Devagondanahalli Hadagali
DH
Shaikh
Sarfaraj Mohd Takhi
SM
Seetharamappa
Jaldappa
J
Harikrishna
Kasalanti
K
D018687:Antidepressive Agents, Second-Generation / Q000032:analysis; D001962:Bromcresol Purple / Q000737:chemistry; D001979:Bromthymol Blue / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012995:Solubility; D013053:Spectrophotometry / Q000379:methods*; D014196:Trazodone / Q000032:analysis* / Q000737:chemistry
D018687:Antidepressive Agents, Second-Generation; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D001962:Bromcresol Purple; D001979:Bromthymol Blue; D014196:Trazodone