title: A new sesquiterpene lactone from the roots of Lasianthus acuminatissimus.
abstract: OBJECTIVE
  To study the active constituents for the treatment of rheumatoid arthritis from the ethyl acetate extracts of the roots of Lasianthus acuminatissimus Merr.
  
  
  METHODS
  Various chromatographic techniques were used to separate and purify the constituents. Their structures were established on the basis of 1D, 2D NMR and HRMS spectroscopic analyses and their preliminary evaluation of anti-inflammation effect on the release of beta-glucuronidase was carried out.
  
  
  RESULTS
  Eight compounds were isolated and identified as lasianthuslactone A (1), codonolactone (2), 2,5-dimethoxy-1, 4-benzoquinone (3), uncargenin A (4), nonadecyl alcohol (5), 13-docosenoic acid (6), tetracosanoic acid (7) and beta-sitosterol (8). Compound 3 showed a significant inhibitory effect on release of beta-glucuronidase rat polymorphous nuclear leukocytes activated by platelet activating factor (PAF).
  
  
  CONCLUSIONS
  Compound 1 is a new one, the others were isolated from the plant for the first time and 3 is one of active anti-inflammation compound in the plant.
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China. lily7077@tom.com
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Zhang
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Luo
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D016227:Benzoquinones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005966:Glucuronidase / Q000378:metabolism; D008968:Molecular Conformation; D015394:Molecular Structure; D009504:Neutrophils / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D019666:Rubiaceae / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016227:Benzoquinones; D012717:Sesquiterpenes; C525365:lasianthuslactone A; D005966:Glucuronidase; C068509:2,5-dimethoxy-4-benzoquinone