title: Acetate oxidation is the dominant methanogenic pathway from acetate in the absence of Methanosaetaceae.
abstract: The oxidation of acetate to hydrogen, and the subsequent conversion of hydrogen and carbon dioxide to methane, has been regarded largely as a niche mechanism occurring at high temperatures or under inhibitory conditions. In this study, 13 anaerobic reactors and sediment from a temperate anaerobic lake were surveyed for their dominant methanogenic population by using fluorescent in situ hybridization and for the degree of acetate oxidation relative to aceticlastic conversion by using radiolabeled [2-14C]acetate in batch incubations. When Methanosaetaceae were not present, acetate oxidation was the dominant methanogenic pathway. Aceticlastic conversion was observed only in the presence of Methanosaetaceae.
authors.affiliation: Institute of Environment & Resources DTU, Technical University of Denmark, Building 113, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Dimitar
authors.initials: D
authors.lastname: Karakashev
authors.forename: Damien J
authors.initials: DJ
authors.lastname: Batstone
authors.forename: Eric
authors.initials: E
authors.lastname: Trably
authors.forename: Irini
authors.initials: I
authors.lastname: Angelidaki
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000693:Anaerobiosis; D005618:Fresh Water / Q000382:microbiology*; D008697:Methane / Q000378:metabolism*; D019640:Methanosarcinales / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA
chemical_list: D000085:Acetates; D008697:Methane