Infectivity variation and genetic diversity among strains of Western equine encephalitis virus.
Variation in infectivity and genetic diversity in the structural proteins were compared among eight strains of Western equine encephalitis virus (WEEV) to investigate WEEV virulence at the molecular level. A lethal intranasal infectivity model of WEEV was developed in adult BALB/c mice. All eight strains examined were 100 % lethal to adult mice in this model, but they varied considerably in the time to death. Based on the time to death, the eight strains could be classified into two pathotypes: a high-virulence pathotype, consisting of strains California, Fleming and McMillan, and a low-virulence pathotype, comprising strains CBA87, Mn548, B11, Mn520 and 71V-1658. To analyse genetic diversity in the structural protein genes, 26S RNAs from these eight strains were cloned and sequenced and found to have > 96 % nucleotide and amino acid identity. A cluster diagram divided the eight WEEV strains into two genotypes that matched the pathotype grouping exactly, suggesting that variation in infectivity can be attributed to genetic diversity in the structural proteins among these eight strains. Furthermore, potential amino acid differences in some positions between the two groups were identified, suggesting that these amino acid variations contributed to the observed differences in virulence.
Nagata
Les P
LP
Chemical and Biological Defence Section, Defence Research and Development Canada - Suffield, Box 4000, Station Main, Medicine Hat, AB T1A 8K6, Canada.
Hu
Wei-Gang
WG
Chemical and Biological Defence Section, Defence Research and Development Canada - Suffield, Box 4000, Station Main, Medicine Hat, AB T1A 8K6, Canada.
Parker
Michael
M
United States Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA.
Chau
Damon
D
Chemical and Biological Defence Section, Defence Research and Development Canada - Suffield, Box 4000, Station Main, Medicine Hat, AB T1A 8K6, Canada.
Rayner
George A
GA
Chemical and Biological Defence Section, Defence Research and Development Canada - Suffield, Box 4000, Station Main, Medicine Hat, AB T1A 8K6, Canada.
Schmaltz
Fay L
FL
Chemical and Biological Defence Section, Defence Research and Development Canada - Suffield, Box 4000, Station Main, Medicine Hat, AB T1A 8K6, Canada.
Wong
Jonathan P
JP
Chemical and Biological Defence Section, Defence Research and Development Canada - Suffield, Box 4000, Station Main, Medicine Hat, AB T1A 8K6, Canada.
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004195:Disease Models, Animal; D004667:Encephalitis Virus, Western Equine / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D004683:Encephalomyelitis, Equine / Q000821:virology; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D016019:Survival Analysis; D013997:Time Factors; D015678:Viral Structural Proteins / Q000235:genetics
D012367:RNA, Viral; D015678:Viral Structural Proteins