title: Inhibition of anaphylaxis-like reaction and mast cell activation by water extract from the fruiting body of Phellinus linteus.
abstract: Mast cell-mediated anaphylactic reaction is involved in many allergic diseases such as asthma and allergic rhinitis. Phellinus linteus has been used as a traditional herb medicine in oriental countries and is known to have anti-tumor, immunomodulatory, anti-inflammatory, and anti-allergic activities. However, roles of Phellinus linteus in the mast cell-mediated anaphylactic reactions have not fully been examined. In the present study, we have investigated the effects of water extract from the fruiting body of Phellinus linteus (WEPL) on mast cell-mediated anaphylaxis-like reactions. Oral administration of WEPL inhibited the compound 48/80-induced systemic anaphylaxis-like reaction and ear swelling response. WEPL also inhibited the anti-dinitrophenyl (DNP) IgE-mediated passive systemic and cutaneous anaphylaxis. WEPL had no cytotoxicity on rat peritoneal mast cells (RPMC). WEPL dose-dependently reduced histamine release from RPMC activated by compound 48/80 or anti-DNP IgE. Moreover, WEPL decreased the compound 48/80-induced calcium uptake into RPMC. Furthermore, WEPL increased the level of intracellular cAMP and significantly inhibited the compound 48/80-induced cAMP reduction in RPMC. These results suggest that WEPL may serve as an effective therapeutic agent for allergic diseases.
authors.affiliation: Department of Anatomy, Chonbuk National University Medical School, Jeonbuk, Republic of Korea.
authors.forename: Yun Ho
authors.initials: YH
authors.lastname: Choi
authors.forename: Guang Hai
authors.initials: GH
authors.lastname: Yan
authors.forename: Ok Hee
authors.initials: OH
authors.lastname: Chai
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lim
authors.forename: So Young
authors.initials: SY
authors.lastname: Sung
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Su Hwan
authors.initials: SH
authors.lastname: Choi
authors.forename: Moo Sam
authors.initials: MS
authors.lastname: Lee
authors.forename: Eui-Hyeog
authors.initials: EH
authors.lastname: Han
authors.forename: Hyoung Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Song
mesh_terms: D000707:Anaphylaxis / Q000517:prevention & control*; D000818:Animals; D001487:Basidiomycota; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D006636:Histamine Release / Q000187:drug effects; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D014867:Water
chemical_list: D004364:Pharmaceutical Preparations; D014867:Water; D002118:Calcium