Soluble Dectin-1 as a tool to detect beta-glucans.
Beta-glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian beta-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.
Graham
Lisa M
LM
Institute of Infectious Disease and Molecular Medicine, CLS, Faculty of Health Sciences, University of Cape Town, Observatory, 7925 Cape Town, South Africa.
Tsoni
S Vicky
SV
Willment
Janet A
JA
Williams
David L
DL
Taylor
Philip R
PR
Gordon
Siamon
S
Dennehy
Kevin
K
Brown
Gordon D
GD
D000918:Antibody Specificity; D002460:Cell Line; D002473:Cell Wall / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000032:analysis*; D005936:Glucans / Q000737:chemistry; D006801:Humans; D037181:Lectins, C-Type; D008565:Membrane Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae; D012995:Solubility; D047071:beta-Glucans / Q000032:analysis* / Q000737:chemistry; D000099181:Dectin-1
D005656:Fungal Proteins; D005936:Glucans; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D047071:beta-Glucans; D000099181:Dectin-1; C480175:glucan phosphate