Serial evolution of TCR beta chain transcript mobilization in HIV type-1-infected patients following vaccine immune stimulation and HAART interruption.
In this article, we studied the T cell receptor (TCR)beta chain transcript mobilization in peripheral blood lymphocytes harvested from HIV-1-infected patients before and after vaccination with a mixture of six lipopeptides and at the moment and serially after highly active antiretroviral therapy (HAART) interruption. This study was performed by using a combined qualitative and quantitative assessment of Vbeta mRNA alterations at the level of complementary determining region 3 length distribution (CDR3-LD) of the TCR. Whereas healthy individuals displayed both stable CDR3-LD profiles and Vbeta transcript accumulations over time, the four HIV-1-infected patients in a quiescent disease phase under HAART have a highly significantly biased CDR3-LD. In addition, they displayed a significant further increase of alterations of their beta CDR3-LD profile after vaccination and both a more altered CDR3-LD (p < 0.05) and an increased transcript accumulation of some Vbeta families after HAART interruption. These modifications mostly concerned the CD8(+ve) T cells. Such a global approach of TCR alterations may help to follow the immune response of these patients and allow targeting of more complex in vivo studies by identifying the T cells with a selected repertoire.
Guille
Marina
M
TcLand, CHU Hôtel-Dieu, 44093 Nantes Cedex 01, France.
Andrieu
Muriel
M
Braudeau
Cécile
C
Ruiz
Catherine
C
Daniel
Nathalie
N
Pallier
Annaïck
A
Charmeteau
Bénédicte
B
Veziers
Joelle
J
Oden
Neal
N
Bonilla
Nelly
N
Brouard
Sophie
S
Guillet
Jean-Gérard
JG
Soulillou
Jean-Paul
JP
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000328:Adult; D023241:Antiretroviral Therapy, Highly Active / Q000379:methods; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D022801:Complementarity Determining Regions / Q000378:metabolism*; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology; D019674:Genes, T-Cell Receptor beta / Q000276:immunology; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008875:Middle Aged; D018807:Polymorphism, Single-Stranded Conformational; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism*; D012044:Regression Analysis; D017422:Sequence Analysis, DNA / Q000379:methods
D016915:AIDS Vaccines; D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta