[Effect of some active Chinese herbal fraction on brain tissue proteomic profile of ischemic mouse].
OBJECTIVE
To study the effect of some active Chinese herbal fraction on protein expression of brain tissue in ischemic mouse with proteomic technique.


METHODS
Ischemia-reperfusion mice were treated with baicalin, geniposide, cholic acid and concha margaritifera respectively for 3 hrs, and then their brain tissue were taken to extract the total protein. Protein expression in ischemic mouse brain was analyzed with surface-enhanced laser desorption/inionation-time of flight-mass spectra (SELDI-TOF-MS) protein-chip.


RESULTS
The four components tested had effect on 3 target proteins at 5373Da, 5707Da and 15103Da, showing the nature of multi-target and with different action on protein expression.


CONCLUSIONS
Protein-chip is an effective approach for exploring the pharmacological mechanism of Chinese herbal fraction.
Song
Yuan-ying
YY
The People's Hospital of Changchun.
Wang
Zhong
Z
Qu
Xun
X
D000818:Animals; D001921:Brain / Q000378:metabolism*; D020520:Brain Infarction / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use*; D005260:Female; D008297:Male; D051379:Mice; D008517:Phytotherapy; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D004365:Drugs, Chinese Herbal