Evaluation of the detection of biomolecules in capillary electrophoresis by contactless conductivity measurement.
The sensitivity of contactless conductivity detection to amino acids, peptides and proteins in CE was studied for BGE solutions of different pH values. The LOD and analytical characteristics were compared for acidic and basic conditions and better results were in most cases found for buffers of low pH values. Linear dynamic ranges varied between two orders of magnitude for amino acids and peptides and three orders of magnitude for larger proteins. The concentration detection limits were found to be between 1.2 and 7.5 microM for the amino acids tested and for the larger molecules they varied between 2.6 microM for leucine enkephalin and 0.2 microM for HSA when using a buffer at pH 2.1.
Abad-Villar
Eva M
EM
Department of Chemistry, University of Basel, Basel, Switzerland.
Kubán
Pavel
P
Hauser
Peter C
PC
D000596:Amino Acids / Q000032:analysis*; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D000596:Amino Acids; D010455:Peptides; D011506:Proteins