Different growth rates of Chlamydia trachomatis biovars reflect pathotype.
BACKGROUND
Despite small genomic differences, Chlamydia trachomatis biovars exhibit diverse disease manifestations and different growth rates in vivo and in cell culture models.


METHODS
Chlamydial inclusion-forming units were enumerated over time in HeLa cells, to evaluate the length of the developmental cycle for C. trachomatis strains A, B, C, and E/Bour (ocular strains) as well as D, E/UW5/Cx, F, and L2 (genital strains). Prototype strains A, D, and L2 were selected for detailed analysis of reticulate body growth, division, and genomic replication. The impact that changing host cells and that coinfection with different strains has on growth was also assessed.


RESULTS
The genital strains completed the developmental cycle in 36-44 h, whereas the ocular strains lagged behind considerably. Differences were the result of a longer lag phase (entry plus differentiation) and generation time for the ocular strains. A prototype ocular strain grew faster in conjunctival cells than in cervical cells. Coinfection with genital (D or L2) and ocular strains expedited recovery of the ocular strain.


CONCLUSIONS
Precise temporal evaluation of the chlamydial developmental cycle for selected genital and ocular C. trachomatis biovars provides a means for investigating genomic differences that define chlamydial pathotype.
Miyairi
Isao
I
Department of Pediatrics, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Mahdi
Olaimatu S
OS
Ouellette
Scot P
SP
Belland
Robert J
RJ
Byrne
Gerald I
GI
D002692:Chlamydia trachomatis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D003228:Conjunctiva / Q000166:cytology / Q000382:microbiology; D048749:Cytokinesis / Q000235:genetics / Q000502:physiology; D004261:DNA Replication; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D010406:Penicillins / Q000494:pharmacology; D014141:Trachoma / Q000382:microbiology; D002575:Uterine Cervicitis / Q000382:microbiology
D010406:Penicillins