title: Protective effect of carvedilol on oxidative stress induced by okadaic acid in N1E-115 cells.
abstract: The effect of carvedilol on oxidative and cell damage induced by okadaic acid in N1E-115 cells were studied. The effects of okadaic acid were evaluated as changes in: the quantity of lipid peroxidation products, protein carbonyl groups, reduced glutathione content (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase and total lactate dehydrogenase (cell LDH). Additionally, a dose of carvedilol (10(-5)M) was added 2h before incubation with okadaic acid (50 nM) and was present until the end of the experiment (2h later added okadaic acid). Our results reveal that okadaic acid induces oxidative stress and an increase of cell LDH in N1E-115 cells, whereas carvedilol prevented the changes prompted by okadaic acid. In conclusion, the data show the protective effect of carvedilol, as well as its ability to modify cell response to okadaic acid, involving like cytoprotective mechanism its antioxidative properties.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, University of Cordoba, Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain. fm2tufii@uco.es
authors.forename: Isaac
authors.initials: I
authors.lastname: Túnez
authors.forename: Juan A
authors.initials: JA
authors.lastname: Collado
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Medina
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Muñoz
authors.forename: Rafael
authors.initials: R
authors.lastname: Gordillo
authors.forename: Concepción
authors.initials: C
authors.lastname: Sampedro
authors.forename: María J
authors.initials: MJ
authors.lastname: Moyano
authors.forename: Montserrat
authors.initials: M
authors.lastname: Feijóo
authors.forename: Jordi
authors.initials: J
authors.lastname: Muntané
authors.forename: Pedro
authors.initials: P
authors.lastname: Montilla
mesh_terms: D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D000077261:Carvedilol; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D004347:Drug Interactions; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008550:Melatonin / Q000378:metabolism; D051379:Mice; D009447:Neuroblastoma; D019319:Okadaic Acid / Q000633:toxicity*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D011412:Propanolamines / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002227:Carbazoles; D011412:Propanolamines; D011506:Proteins; D000077261:Carvedilol; D019319:Okadaic Acid; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D008550:Melatonin