SAP deficiency results in a striking alteration of the protein profile in activated CD4 T cells.
Deficiency in a protein called signaling lymphocytic activation molecule-associated protein (SAP) causes X-linked lymphoproliferative disease (XLP) and helper T cell-dependent antibody defects. To identify proteins regulated by SAP, we performed proteomic analyses of SAP deficient vs wild type T cells. Our results reveal protein species whose abundances are profoundly altered by SAP. Our work therefore identifies candidate cellular mediators of SAP-dependent T cell help.
Kamperschroer
Cris
C
Trudeau Institute, Saranac Lake, New York, USA.
Swain
Susan L
SL
Grussenmeyer
Thomas
T
Lefkovits
Ivan
I
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry*; D002478:Cells, Cultured; D047908:Intracellular Signaling Peptides and Proteins / Q000172:deficiency* / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis* / Q000235:genetics*; D040901:Proteomics / Q000379:methods; D013601:T-Lymphocytes / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism
D000945:Antigens, Differentiation, T-Lymphocyte; D047908:Intracellular Signaling Peptides and Proteins; D020543:Proteome