Replication of bovine respiratory syncytial virus in murine cells depends on type I interferon-receptor functionality.
Bovine respiratory syncytial virus (BRSV) is able to counteract the alpha/beta interferon (IFN-alpha/beta)-mediated antiviral response for efficient replication in a host-specific manner. Mice models have been developed for experimental infection with human, but not bovine, respiratory syncytial virus strains. Here, it is shown that BRSV can replicate efficiently on primary cell cultures derived from type I IFN receptor-deficient, but not from wild-type IFN-competent, mice. However, BRSV infection was not enhanced in mice devoid of the type I IFN receptor. These results show that type I IFN is a major host-range determinant for infection at the cellular level, but that other factors control virus replication and pathology in vivo.
Riffault
Sabine
S
Unité de Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
Dubuquoy
Catherine
C
Unité de Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
Castagné
Nathalie
N
Unité de Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
Baranowski
Eric
E
UMR1225, École nationale vétérinaire de Toulouse (ENVT), 31076 Toulouse, France.
Charley
Bernard
B
Unité de Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
Eléouët
Jean-François
JF
Unité de Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
D000818:Animals; D002478:Cells, Cultured; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence; D053633:Receptor, Interferon alpha-beta; D017471:Receptors, Interferon / Q000235:genetics / Q000502:physiology*; D018357:Respiratory Syncytial Virus Infections / Q000821:virology*; D018112:Respiratory Syncytial Virus, Bovine / Q000502:physiology*; D014779:Virus Replication
D008565:Membrane Proteins; D017471:Receptors, Interferon; D053633:Receptor, Interferon alpha-beta