Thermovirga lienii gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, amino-acid-degrading bacterium isolated from a North Sea oil well.
A novel anaerobic, moderately thermophilic bacterium, strain Cas60314(T), was isolated from hot oil-well production water obtained from an oil reservoir in the North Sea. The cells were Gram-negative, motile, straight rods. The salinity and pH growth optima were 2.0-3.0 % NaCl and 6.5-7.0, respectively. The optimum temperature was 58 degrees C. Strain Cas60314(T) had a fermentative type of metabolism and utilized proteinous substrates, some single amino acids and a limited number of organic acids, but not sugars, fatty acids or alcohols. Cystine and elemental sulfur were reduced to sulfide. The G+C content of the DNA was 46.6 mol%. On the basis of phenotypic and phylogenetic features, it is proposed that this isolate represents a novel genus and species with the name Thermovirga lienii gen. nov., sp. nov. within the family Syntrophomonadaceae. The proposed type strain is strain Cas60314(T) (=DSM 17291(T)=ATTC BAA-1197(T)).
Dahle
Håkon
H
Department of Biology, University of Bergen, PO Box 7800, N-5020 Bergen, Norway.
Birkeland
Nils-Kåre
NK
Department of Biology, University of Bergen, PO Box 7800, N-5020 Bergen, Norway.
D000438:Alcohols / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation; D020459:Genes, rRNA; D005840:Gentian Violet; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D006131:Growth Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009068:Movement; D009659:North Sea; D010084:Oxidation-Reduction; D010578:Petroleum; D010619:Phenazines; D010802:Phylogeny; D011506:Proteins / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D013455:Sulfur / Q000378:metabolism; D013696:Temperature
D000438:Alcohols; D000596:Amino Acids; D002264:Carboxylic Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D006131:Growth Inhibitors; D010578:Petroleum; D010619:Phenazines; D011506:Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D013455:Sulfur; D005840:Gentian Violet; D003545:Cysteine