Degradation of scrapie associated prion protein (PrPSc) by the gastrointestinal microbiota of cattle.
A food-borne origin of the transmission of bovine spongiform encephalopathy (BSE) to cattle is commonly assumed. However, the fate of infectious prion protein during polygastric digestion remains unclear. It is unknown at present, whether infectious prion proteins, considered to be very stable, are degraded or inactivated by microbial processes in the gastrointestinal tract of cattle. In this study, rumen and colon contents from healthy cattle, taken immediately after slaughter, were used to assess the ability of these microbial consortia to degrade PrP(Sc). Therefore, the consortia were incubated with brain homogenates of scrapie (strain 263K) infected hamsters under physiological anaerobic conditions at 37 degrees C. Within 20 h, PrP(Sc) was digested both with ruminal and colonic microbiota up to immunochemically undetectable levels. Especially polymyxin resistant (mainly gram-positive) bacteria expressed PrP(Sc) degrading activity. These data demonstrate the ability of bovine gastrointestinal microbiota to degrade PrP(Sc) during digestion.
Scherbel
Christina
C
Institute for Microbiology and Toxicology, Federal Research Centre for Nutrition and Food, E.-C. Baumann Str. 20, 95326 Kulmbach, Germany.
Pichner
Rohtraud
R
Groschup
Martin H
MH
Mueller-Hellwig
Simone
S
Scherer
Siegfried
S
Dietrich
Richard
R
Maertlbauer
Erwin
E
Gareis
Manfred
M
D000818:Animals; D002417:Cattle; D003106:Colon / Q000382:microbiology*; D006224:Cricetinae; D004063:Digestion; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism / Q000635:transmission; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011328:Prions / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*; D012608:Scrapie / Q000378:metabolism / Q000635:transmission
D011328:Prions