Region-dependent differences and alterations of protective thiol compound levels in cultured astrocytes and brain tissues.
We examined region-dependent differences and alterations in the levels of protective thiol compounds, glutathione (GSH) and metallothionein (MT)-I and -II, in cultured rat astrocytes under several culture conditions and in brain tissues of rats at postnatal and weaning periods. Regardless of culture conditions, both protein concentrations and mRNA expressions of MT-I and -II were much higher in the cerebral hemisphere than in cerebellar astrocytes, whereas no difference was observed in GSH concentration. In both astrocytes, the GSH concentrations did not change within 12 h but significantly increased 24 h after being maintained in a serum-free defined medium. At 24 h, protein concentrations and mRNA expressions of MT-I and -II also increased in the respective astrocytes, and were further enhanced when maintained in the presence of 50 microM Zn(2+). In the brain tissues, the MT-I/-II protein concentrations were significantly higher in the cerebral cortex (a part of the cerebral hemisphere) than in the cerebellum, whereas the GSH concentration was similar at both postnatal day (P)1 and P35. In addition, the concentrations in the respective regions were significantly higher at P35 than at P1. These results suggest that region-dependent differences in the cellular levels of GSH and MTs in cultured astrocytes might reflect the in vivo differences, and that the levels of the respective thiol compounds in cultured astrocytes increase after serum elimination along with the region-dependent differences.
Adachi
Tatsumi
T
National Institute for Environmental Studies, Ibraki, Japan. tadachi@cis.ac.jp
Satoh
Masahiko
M
Pramanik
Rocky
R
Kuroda
Sakiko
S
Ishido
Masami
M
Kunimoto
Manabu
M
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D013438:Sulfhydryl Compounds; D008668:Metallothionein; D005978:Glutathione