title: Protein kinase Calpha and sphingosine 1-phosphate-dependent signaling in endothelial cell.
abstract: Protein kinase C (PKC)-mediated signal transduction pathways convert extracellular stimulation into a variety of cellular functions. However, the role of various PKC isoforms in sphingosine 1-phosphate (S1P)-stimulated endothelial cells is not well understood. PKCalpha and PKCepsilon activity are increased in endothelial cell cultures, and S1P receptor transfection studies indicate S1P(3) stimulates PKCalpha and S1P1 leads to PKCepsilon activity. Infection of endothelial cells with dominant negative (DN)PKCalpha adenovirus reduces cell migration and greatly inhibits morphogenesis in cells stimulated with S1P. This effect is specific to PKCalpha, as infection with DN PKCepsilon does not alter either migration or morphogenesis. The PKC-specific chemical inhibitor GF109203X also inhibits these two responses. Infection of endothelial cells with dominant negative PKCalpha reduces S1P-induced calcium rise. This maximal rise requires calcium uptake, but it does not require enzymatic activity of the kinase. Pretreatment of these cells with the PKC-specific inhibitor GF109203X does not inhibit S1P-induced calcium rise. S1P-induced morphogenesis but not cell migration is critically dependent on extracellular calcium. Pretreatment of endothelial cells with phorbol 12-myristate 13-acetate for 5min abolishes S1P-stimulated rise in calcium but had little or no effect on migration. The PMA-inhibited calcium rise can be prevented by PKC inhibitor or infection with dominant negative PKCalpha.
authors.affiliation: Department of Physiology, University of Connecticut Health Center, Farmington, CT 06030-3505, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Thompson
authors.forename: Nicolas
authors.initials: N
authors.lastname: Ancellin
authors.forename: Salvador M
authors.initials: SM
authors.lastname: Fernandez
authors.forename: Tim
authors.initials: T
authors.lastname: Hla
authors.forename: Ramadan I
authors.initials: RI
authors.lastname: Sha'afi
mesh_terms: D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D004789:Enzyme Activation; D006801:Humans; D008246:Lysophospholipids / Q000502:physiology*; D051571:Protein Kinase C-alpha / Q000235:genetics / Q000502:physiology*; D051744:Protein Kinase C-epsilon / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; D051571:Protein Kinase C-alpha; D051744:Protein Kinase C-epsilon; D013110:Sphingosine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium