[Rapid centrifugation for routine coagulation testing].
Routine coagulation assays are performed with platelet-poor plasma obtained after centrifugation of whole citrated blood. Usually clinical laboratories centrifuge blood from 2,000 to 2,500 g for 15-30 minutes. Thirty two blood samples routinely submitted to coagulation tests, were assayed for the prothrombin time, activated partial thromboplastin time, and fibrinogen level, in order to compare results obtained using 2 types of centrifugation : centrifugation at 2,500 g for 15 minutes and rapid centrifugation on StatSpin Express 2 at 4,440 g for 2 minutes. A good correlation was observed for the prothrombin time, activated partial thromboplastin time, and fibrinogen levels being respectively 1,009, 0,908 and 1. We concluded that rapid centrifugation at 4,440 g for 2 minutes does not modify results and contributes, by decreasing duration of the pre-analytical variable to reduce the completion time of these tests.
Boudaoud
L
L
Service d'Hématologie Biologique, Hôpital Beaujon, Clichy. larbi.boudaoud@bjn.aphp.fr
Divaret
G
G
Marie
P
P
Bezeaud
A
A
D001780:Blood Coagulation Tests / Q000379:methods*; D001800:Blood Specimen Collection / Q000379:methods; D002498:Centrifugation / Q000379:methods*; D005340:Fibrinogen / Q000378:metabolism; D006487:Hemostasis; D006801:Humans; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D012016:Reference Values
D005340:Fibrinogen