Achieving directed immunostimulating complexes incorporation.
In recent years, several studies have been reported with the common aim of generating general expression systems for straightforward production and subsequent coupling of expressed antigens to an adjuvant system. Here, we describe a series of such efforts with a common theme of using gene fusion technology for association of recombinant antigens to immunostimulating complexes (iscoms). In the early stages of vaccine development, uniform antigen preparations are crucial to allow the comparison of immune responses to different antigens, or even subdomains thereof, and we believe that the described systems constitute an important development in this context.
Wikman
Maria
M
AlbaNova University Center, Department of Biotechnology, Kungl Tekniska Högskolan (KTH), SE-10691 Stockholm, Sweden. maria.wikman@biotech.kth.se
Friedman
Mikaela
M
Pinitkiatisakul
Sunan
S
Andersson
Christin
C
Lövgren-Bengtsson
Karin
K
Lundén
Anna
A
Ståhl
Stefan
S
D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D016882:ISCOMs / Q000737:chemistry / Q000276:immunology*; D008074:Lipoproteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010455:Peptides / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D022223:Vaccines, Subunit / Q000276:immunology*
D000941:Antigens; D001426:Bacterial Proteins; D016882:ISCOMs; D008074:Lipoproteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D022223:Vaccines, Subunit; C066114:biotin-streptavidin complex; D001710:Biotin