Comparison of the Phytotoxkit microbiotest and chemical variables for toxicity evaluation of sediments.
The main objective of the research was to evaluate the suitability of the Phytotoxkit microbiotest as a tool for hazard assessment of sediments. The concentrations of oil derivatives, polycyclic aromatic hydrocarbons (PAHs), and heavy metals (Cd, Cr, Cu, Hg, Ni, Pb, and Zn) were determined in sediment samples collected from the urban canal in Opole (Poland), in order to obtain a general insight of the level of sediment contamination. Phytotoxicity of sediments was estimated on the basis of seed germination and root elongation measurements, combined into an overall germination index (GI). The results revealed spatial and vertical differentiation in sediment contamination. A good correlation was obtained between organic matter content and the concentrations of particular sediment pollutants. Values of correlation coefficients at P < 0.05 ranged from 0.3246 for oil derivatives to 0.8929 for PAHs. Phytotoxicity tests, carried out on the monocotyl Sorghum saccharatum and the dicotyls Sinapis alba and Lepidium sativum, showed different responses of the three plant species to sediment samples ranging from growth inhibition to growth stimulation. The GI values revealed the following increasing order of plant sensitivity to contaminated sediments: L. sativum < S. alba < S. saccharatum. The study demonstrated that the Phytotoxkit microbiotest was effective in identifying toxic samples. However, sediment organic matter content and grain-size distribution had a significant impact on both sediment contamination and higher plantresponses to contaminated samples. The implication of these findings are discussed.
Czerniawska-Kusza
Izabela
I
Department of Land Protection, Opole University, Oleska 22, 45-052 Opole, Poland. Izeabela.Kusza@uni.opole.pl
Ciesielczuk
Tomasz
T
Kusza
Grzegorz
G
Cichoń
Aleksandra
A
D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis / Q000633:toxicity*; D019015:Geologic Sediments; D018525:Germination / Q000187:drug effects; D031220:Lepidium sativum / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity; D010316:Particle Size; D011044:Poland; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D012639:Seeds / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development; D031226:Sinapis / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development; D045868:Sorghum / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development; D018675:Toxicity Tests / Q000379:methods*
D004785:Environmental Pollutants; D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons