title: [Antenatal determination of fetal RhD-blood type based on fetal DNA in plasma from the RhD-negative mother--secondary publication].
abstract: We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD-women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th-36th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.
authors.affiliation: H:S Rigshospitalet, H:S Blodbank, KI 2034, DK-2100 København Ø. frederik.banch.clausen@rh.dk
authors.forename: Frederik Banch
authors.initials: FB
authors.lastname: Clausen
authors.forename: Grethe Risum
authors.initials: GR
authors.lastname: Krog
authors.forename: Klaus
authors.initials: K
authors.lastname: Rieneck
authors.forename: Leif Kofoed
authors.initials: LK
authors.lastname: Nielsen
authors.forename: Rasmus
authors.initials: R
authors.lastname: Lundquist
authors.forename: Kirsten
authors.initials: K
authors.lastname: Finning
authors.forename: Ebbe
authors.initials: E
authors.lastname: Dickmeiss
authors.forename: Morten
authors.initials: M
authors.lastname: Hedegaard
authors.forename: Morten Hanefeld
authors.initials: MH
authors.lastname: Dziegiel
mesh_terms: D004247:DNA / Q000097:blood*; D015342:DNA Probes; D005091:Exons; D005260:Female; D005315:Fetal Diseases / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D012204:Rh-Hr Blood-Group System; D004247:DNA