title: Genetic relatedness and antimicrobial resistance determinants among clinical isolates of enterococci from Cuba.
abstract: This study describes the genetic relationships and antimicrobial resistance determinants found among 99 clinical isolates of enterococci from 15 different hospitals in Cuba. Pulsed-field gel electrophoresis SmaI analysis demonstrated a high degree of genetic diversity. A limited number of multiresistant Enterococcus faecalis clones, showing resistance to three or more families of antimicrobial agents, were detected simultaneously in different institutions, suggesting inter-hospital circulation of selected clones, and/or selection of particular clones following their introduction into the hospital environment. Antimicrobial resistance determinants, including erm(B), aac(6')-aph(2'), aph(3'), ant(6), vanB (E. faecalis) and vanA (Enterococcus faecium) were detected by PCR in various isolates.
authors.affiliation: Department of Bacteriology-Mycology, Tropical Medicine Institute Pedro Kourí, Havana City, Cuba.
authors.forename: D
authors.initials: D
authors.lastname: Quiñones-Pérez
authors.forename: P
authors.initials: P
authors.lastname: Goñi
authors.forename: M C
authors.initials: MC
authors.lastname: Rubio
authors.forename: F
authors.initials: F
authors.lastname: Baquero
authors.forename: R
authors.initials: R
authors.lastname: Gómez-Lus
authors.forename: R
authors.initials: R
authors.lastname: Del Campo
mesh_terms: D000617:Aminoglycosides / Q000378:metabolism / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics*; D006801:Humans
chemical_list: D000617:Aminoglycosides