[Effect of dahuang zhechong pill on content of amino acids in hippocampal tissue of ischemic cerebral hemorrhagic rats].
OBJECTIVE
To observe the effect of Dahuang Zhechong Pill (DZP) on the content of amino acids in hippocampal tissue of model rats of ischemic cerebral hemorrhage (ICH).


METHODS
Collagenase and heparin were injected into caudate nucleus to establish ICH rat model. The content of glutamic acid (Glu), aspartic acid (Asp) and gamma-aminobutyric acid (GABA) in hippocampal tissue was detected by anti-phase high-performance liquid chromatographic fluorimetry to observe the effect of DZP on the content of amino acids in brain tissue.


RESULTS
Three days after modeling, the content of Glu, Asp and GABA was significantly higher in the model group than those in the sham-operation and the normal control group (P < 0.05), for Glu 15.2926 +/- 4.2429 micromol/g vs 8.0057 +/- 1.1227 micromol/g and 8.5040 +/- 1.7794 micromol/g, for Asp 3.6384 +/- 3.1021 micromol/g vs 1.4986 +/- 0.4174 micromol/g and 1.2669 +/- 0.4695 micromol/g, and for GABA 0.3859 +/- 0.1846 micromol/g vs 0.1829 +/- 0.04665 micromol/g and 0.1770 +/- 0.0472 micromol/g. The content of Glu (9.0550 +/- 1.7195 micromol/g), Asp (1.8085 +/- 0.6862 micromol/g) and GABA (0.1993 +/- 0.0424 micromol/g) in hippocampal tissue in the DZP group was reduced significantly, as compared with that in the model group (P <0.05).


CONCLUSIONS
DZP could reduce the release of excitatory amino acids (EAA-Glu, ASP) to balance EAA/inhibitory amino acid (IAA), so as to relieve ICH-induced brain injury.
Dai
Gao-zhong
GZ
Department of Internal Medicine, Wuxi Traditional Chinese Medicine Hospital, Jiangsu. daigaozhong@126.com
Chen
Ru-xing
RX
Wei
Hong-chang
HC
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000596:Amino Acids; C521634:Dahuang Zhechong pill; D004365:Drugs, Chinese Herbal; D001224:Aspartic Acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid