title: No abnormal prion protein detected in the milk of cattle infected with the bovine spongiform encephalopathy agent.
abstract: Milk specimens were collected from lactating cows that had previously been challenged with bovine spongiform encephalopathy (BSE)-infected brain at 4-6 months of age. One group of 10 animals received a single oral dose of 100 g, a second group received 1 g and the third was made up of unexposed controls. The cows were inseminated artificially, and calved at approximately 2 years of age and annually thereafter. Milking was done within the first week following calving and at 10-weekly intervals during the lactation period. Specimens were centrifuged to obtain a fraction enriched for somatic cells and these fractions were analysed for disease-associated, abnormal prion protein (PrP(BSE)) by using a modified commercial BSE ELISA and a different confirmatory assay. No abnormal prion protein has so far been identified in the cell fraction of milk from cattle incubating BSE by using these methods at their limits of detection.
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Sally J
authors.initials: SJ
authors.lastname: Everest
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Leigh T
authors.initials: LT
authors.lastname: Thorne
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Hawthorn
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Russell
authors.initials: R
authors.lastname: Jenkins
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Clare
authors.initials: C
authors.lastname: Hammersley
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Ramsay
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Hawkins
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Venables
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Linda
authors.initials: L
authors.lastname: Flynn
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Robin
authors.initials: R
authors.lastname: Sayers
authors.affiliation: ADAS Rosemaund, Preston Wynne, Hereford HR1 3PG, UK.
authors.forename: John
authors.initials: J
authors.lastname: Kilpatrick
authors.affiliation: ADAS Defra Drayton, Stratford-upon-Avon, Warwickshire CV37 9RQ, UK.
authors.forename: Amanda
authors.initials: A
authors.lastname: Sach
authors.affiliation: VLA Lasswade, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: James
authors.initials: J
authors.lastname: Hope
authors.affiliation: Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Roy
authors.initials: R
authors.lastname: Jackman
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008892:Milk / Q000737:chemistry*; D011328:Prions / Q000032:analysis*
chemical_list: D011328:Prions