Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CpG DNA stimulation.
OBJECTIVE
To investigate the features of metabolic activation induced by bacterial CpG DNA (bCpG DNA) in peritoneal macrophages (PMphis).


METHODS
Electron paramagnetic resonance spin-trapping technique using respective spin traps was applied to study the generation rate of reactive oxygen species and NO production by PMphis of BALB/c mice.


RESULTS
For the first time the capability of bCpG DNA isolated from Bacillus subtilis GP1-807-03 culture medium to elevate activity of NADP composite functionH oxidase and inducible NO synthase in PMphis of normal and tumor-bearing mice have been demonstrated. The main differences in superoxide anion generation rate and production of NO by PMphis of normal mice and mice with transplanted solid Ehrlich carcinoma were showed. The effects of bCpG DNA stimulation in vitro on ROS and NO production by PMphis depended on concentration and time exposure with bCpG DNA. Furthermore, response of PMphis from tumor-bearing mice on bCpG DNA stimulation was delayed as compared to PMphis of normal mice.


CONCLUSIONS
The present findings suggest that bCpG DNA have modulatory effect on ROS/NO production by PMphis from normal and tumor-bearing animals.
Olishevsky
S
S
R.E. KavetskyInstitute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine. sergeyolishevsky@yahoo.com
Burlaka
A
A
Sidorik
E
E
Shlyakhovenko
V
V
Garpenko
Yu
Y
Kozak
V
V
D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology*; D018899:CpG Islands; D004269:DNA, Bacterial / Q000008:administration & dosage*; D004578:Electron Spin Resonance Spectroscopy; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019255:NADPH Oxidases / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D026082:Singlet Oxygen / Q000378:metabolism*
D004269:DNA, Bacterial; D017382:Reactive Oxygen Species; D026082:Singlet Oxygen; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D019255:NADPH Oxidases