title: Quantitative analysis of beta-sitosterol oxides induced in vegetable oils by natural sunlight, artificially generated light, and irradiation.
abstract: UV radiation is able to induce lipid peroxidation. Photooxidation-induced beta-sitosterol oxides were monitored in four vegetable oils exposed to sunlight for 10, 20, and 30 days during May 2005 (northeastern France), exposed to artificial light generated by a high-pressure Hg lamp for 21, 42, and 63 h at room temperature, and exposed to a 10 MeV electron beam at 0.93, 2.69, and 9.30 kGy at 8 degrees C. Quantification was performed by capillary gas chromatography-mass spectrometry according to the total ion current mode and using a reconstructed ion trace chromatogram with specific ion fragments. Sunlight induced the formation of higher amounts of oxides than UV light, while no significant oxidizing effect was observed with electron beam irradiation. However, data suggested that the amount of the main oxides formed was strongly dependent on the dose rate (length of exposure). Accordingly, shorter but more intense treatments had lower oxidizing effects.
authors.affiliation: Laboratoire de Chimie Analytique et Sciences de l'Aliment (UMR 7178), Faculté de Pharmacie, Université Louis Pasteur, Illkirch, and Laboratoire d'Oncologie Nutritionnelle, EA 3430/IRCAD, 1 place de l'Hôpital, Strasbourg, France.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Diane
authors.initials: D
authors.lastname: Julien-David
authors.forename: Michel
authors.initials: M
authors.lastname: Miesch
authors.forename: Francis
authors.initials: F
authors.lastname: Raul
authors.forename: Philippe
authors.initials: P
authors.lastname: Geoffroy
authors.forename: Dalal
authors.initials: D
authors.lastname: Aoude-Werner
authors.forename: Saïd
authors.initials: S
authors.lastname: Ennahar
authors.forename: Eric
authors.initials: E
authors.lastname: Marchioni
mesh_terms: D005229:Fatty Acids, Monounsaturated; D008401:Gas Chromatography-Mass Spectrometry; D008027:Light; D015227:Lipid Peroxidation / Q000528:radiation effects; D000069463:Olive Oil; D010087:Oxides / Q000032:analysis*; D010777:Photochemistry; D010938:Plant Oils / Q000737:chemistry* / Q000528:radiation effects*; D000074262:Rapeseed Oil; D012855:Sitosterols / Q000032:analysis* / Q000737:chemistry*; D013024:Soybean Oil / Q000737:chemistry; D000074242:Sunflower Oil; D013472:Sunlight; D014466:Ultraviolet Rays
chemical_list: D005229:Fatty Acids, Monounsaturated; D000069463:Olive Oil; D010087:Oxides; D010938:Plant Oils; D000074262:Rapeseed Oil; D012855:Sitosterols; D000074242:Sunflower Oil; C025473:gamma-sitosterol; D013024:Soybean Oil