Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) and guanylate kinase 1 (GUK1) are differentially expressed in GH-secreting adenomas.
Pituitary tumors, adenomas in their vast majority, represent around 10-15% of the intracranial neoplasms. Pituitary carcinomas are exceedingly rare. Clinically, these neoplasms cause hormonal dysfunctions, and mass effect symptoms as headache and visual disorders in the case of macroadenomas. Pituitary tumorigenesis is still poorly understood. In order to investigate the expression of cancer-related genes in pituitary tumors, we employed a human cancer cDNA macroarray membrane with 1176 well-characterized human genes related to cancer and tumor biology. We were able to identify several differentially expressed genes, among them hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) and guanylate kinase 1 (GUK1) which were over expressed in a pool of clinically nonfunctioning pituitary adenomas, compared with a spinal cord metastasis of a nonfunctioning pituitary carcinoma. HGS and GUK1 mRNA expression were chosen to be validated by quantitative RT-qPCR, however, only GUK1 had the differential expression confirmed between the adenomas and the metastasis of a pituitary carcinoma. We have also investigated HGS and GUK1 mRNA expressions in a series of 46 pituitary adenomas (18 nonfunctioning, 12 GH-secreting, nine PRL-secreting, and seven ACTH-secreting adenomas). HGS and GUK1 were significantly over expressed in GH-secreting adenomas, compared with ACTH-secreting adenomas and nonfunctioning tumors, and with PRL-secreting adenomas, respectively. We have shown that these genes, involved in tumorigenesis in other tissues, are as well over expressed in the pituitary tumors, however, their role in the oncogenesis of these tumors need to be further investigated.
da Rocha
Anderson Alves
AA
Laboratory for Cellular and Molecular Endocrinology-LIM/25, University of Sao Paulo Medical School, Sao Paulo-SP, Brazil.
Giorgi
Ricardo Rodrigues
RR
de Sa
Sandra Valeria
SV
Correa-Giannella
Maria Lucia
ML
Fortes
Maria Angela
MA
Cavaleiro
Ana Mercedes
AM
Machado
Marcio Carlos
MC
Cescato
Valter Angelo
VA
Bronstein
Marcello Delano
MD
Giannella-Neto
Daniel
D
D049913:ACTH-Secreting Pituitary Adenoma / Q000235:genetics / Q000378:metabolism; D000236:Adenoma / Q000235:genetics / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D004273:DNA, Neoplasm / Q000235:genetics; D056827:Endosomal Sorting Complexes Required for Transport; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D049912:Growth Hormone-Secreting Pituitary Adenoma / Q000235:genetics / Q000378:metabolism*; D051528:Guanylate Kinases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D015175:Prolactinoma / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
D004273:DNA, Neoplasm; D056827:Endosomal Sorting Complexes Required for Transport; D010750:Phosphoproteins; D012333:RNA, Messenger; C096164:hepatocyte growth factor-regulated tyrosine kinase substrate; D051528:Guanylate Kinases