A norbergenin derivative inhibits neuronal cell damage induced by tunicamycin.
Several chemically synthesized compounds were examined for protective effects against the cell damage in tunicamycin-treated human neuroblastoma IMR-32 cells. Among the compounds tested, an antioxidant, Norbergenin-11-caproate (10 microM), exhibited complete protection against the cell growth inhibitory effect of tunicamycin but did not inhibit the induction of Bip/GRP78 mRNA by tunicamycin. Both norbergenin-11-caproate and alpha-tocopherol completely inhibited the production of reactive oxygen species induced by tunicamycin, however, alpha-tocopherol inhibited tunicamycin-induced cell damage only partially, even at 100 microM. These findings suggest the potential of Norbergenin-11-caproate for therapeutic application in endoplasmic reticulum (ER) stress-dependent diseases implicating a specific mechanism other than anti-oxidative one.
Suzuki
Shinya
S
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan. suzukis@ph.bunri-u.ac.jp
Okuse
Yui
Y
Kawase
Masafumi
M
Takiguchi
Masufumi
M
Fukuyama
Yoshiyasu
Y
Takahashi
Hironobu
H
Sato
Masao
M
D001578:Benzopyrans / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D017931:DNA Primers; D004365:Drugs, Chinese Herbal; D000091342:Endoplasmic Reticulum Chaperone BiP; D005434:Flow Cytometry; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007564:Japan; D018832:Molecular Chaperones / Q000235:genetics; D009447:Neuroblastoma; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014415:Tunicamycin / Q000633:toxicity*
D001578:Benzopyrans; D017931:DNA Primers; D004365:Drugs, Chinese Herbal; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D017382:Reactive Oxygen Species; D014415:Tunicamycin; C063922:norbergenin