title: Paenibacillus sp. strain HC1 xylanases responsible for degradation of rice bran hemicellulose.
abstract: Paenibacillus sp. strain HC1 is the first bacterium capable of growing on rice bran hemicellulose as a sole carbon source. Two xylanases (Xyl-I and -II) were purified from the bacterial culture fluid and enzymatically characterized. Xyl-I and -II showed monomer forms with molecular masses of 30 and 18kDa, respectively, and were most active at around pH 5.0 and 45 degrees C. Xylooligosaccharides were degraded to xylobiose and xylose by Xyl-I, but not by Xyl-II, suggesting that Xyl-I plays an important role in complete depolymerization of xylan. Both enzymes acted endolytically on rice bran hemicellulose, indicating that Xyl-I and -II contribute to the structure determination and practical use of the polysaccharide, an unutilized biomass in technology.
authors.affiliation: Laboratory of Basic and Applied Molecular Biotechnology, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan.
authors.forename: Karen Mine
authors.initials: KM
authors.lastname: Harada
authors.forename: Keiko
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Fukuda
authors.forename: Wataru
authors.initials: W
authors.lastname: Hashimoto
authors.forename: Kousaku
authors.initials: K
authors.lastname: Murata
mesh_terms: D004043:Dietary Fiber / Q000378:metabolism*; D004187:Disaccharides / Q000378:metabolism; D004795:Enzyme Stability; D006094:Gram-Positive Bacteria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism; D012275:Oryza / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D013696:Temperature; D014994:Xylose / Q000378:metabolism; D014995:Xylosidases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D004043:Dietary Fiber; D004187:Disaccharides; D009844:Oligosaccharides; D011134:Polysaccharides; C007916:hemicellulose; D014994:Xylose; D014995:Xylosidases; C004173:xylobiose