Culture-independent molecular subtyping of Mycoplasma pneumoniae in clinical samples.
A new molecular subtyping approach was developed which is based on the amplification and sequencing of a repetitive region of the P1 gene of Mycoplasma pneumoniae. It allows the differentiation of all known subtypes and variants of M. pneumoniae as well as the identification of new subtypes directly in clinical samples to characterize endemic and epidemic M. pneumoniae infections.
Dumke
Roger
R
Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany. roger.dumke@mailbox.tu-dresden.de
Lück
Paul Christian
PC
Noppen
Christoph
C
Schaefer
Christoph
C
von Baum
Heike
H
Marre
Reinhard
R
Jacobs
Enno
E
D018829:Adhesins, Bacterial / Q000145:classification / Q000235:genetics; D000595:Amino Acid Sequence; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods*; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D011019:Pneumonia, Mycoplasma / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
D018829:Adhesins, Bacterial; D004269:DNA, Bacterial; C035471:adhesin, Mycoplasma pneumoniae