Adjuvant properties of lipopolysaccharide from Neisseria meningitidis serogroup B detoxified and conjugated with tetanus toxoid.
We evaluated the adjuvant properties and toxicity of purified Neisseria meningitidis serogroup B lipopolysaccharide (LPS) conjugated with tetanus toxoid (TT) using a new method of conjugation to obtain amine groups in the polysaccharide structure. The endotoxic activity of treated LPS was reduced 2400 times as determined by Limulus amoebocyte assay and no mortality was observed in Balb/c mice inoculated with detoxified LPS versus 100% mortality in native LPS inoculated mice. The conjugated LPS-TT elicited in mice higher anti-TT IgG2a and IgG1 than unconjugated TT. In addition, high levels of anti-LPS IgG and IgG subclasses were detected in sera. These results evidence the adjuvant activity of detoxified LPS and may suggest that the conjugation to TT changes the LPS immune response from thymus-independent to thymus-dependent.
Balboa
Julio A
JA
Immunology Department, Finlay Institute, P.O. Box 16017, Havana, Cuba jbalboa@finlay.edu.cu
Cuello
Maribel
M
Cabrera
Osmir
O
del Campo
Judith
J
Lastre
Miriam
M
Gil
Danay
D
Taboada
Carlos
C
Fariñas
Mildrey
M
Hernández
Marixa
M
Pérez
Oliver
O
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D038541:Neisseria meningitidis, Serogroup B; D013745:Tetanus Toxoid / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000276:immunology
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D013745:Tetanus Toxoid; D018074:Vaccines, Conjugate