Optimization of process parameters for reduction of gossypol levels in cottonseed meal by Candida tropicalis ZD-3 during solid substrate fermentation.
The objective of this work is to optimize the process parameters for detoxification of gossypol in cottonseed meal (CSM) by Candida tropicalis ZD-3 during solid substrate fermentation (SSF). The maximum detoxification efficiency of gossypol was achieved by employing the substrate, which consists of 70% of CSM, 20% of corn flour and 10% of wheat bran. The optimum fermentation conditions for gossypol detoxification are incubation period of 48h, incubation temperature at 30 degrees Celsius, inoculum level 5% v/w, moisture content of solid substrate 50% and pH in nature. Adding minerals solution to CSM substrate benefit fermentation detoxification.
Zhang
Wen-Ju
WJ
Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Animal Science College, Zhejiang University, Hangzhou 310029, PR China.
Xu
Zi-Rong
ZR
Zhao
Shun-Hong
SH
Jiang
Jun-Fang
JF
Wang
Yan-Bo
YB
Yan
Xiang-Hua
XH
D000821:Animal Feed / Q000032:analysis; D041022:Candida tropicalis / Q000378:metabolism*; D003369:Cottonseed Oil / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D005516:Food Microbiology; D003368:Gossypium; D006072:Gossypol / Q000032:analysis / Q000378:metabolism*; D008658:Inactivation, Metabolic
D003369:Cottonseed Oil; D006072:Gossypol