Structural property of DNA that migrates faster in gel electrophoresis, as deduced by CD spectroscopy.
Bent DNAs are known to migrate slower than ordinary DNA in non-denaturing polyacrylamide gel electrophoresis. In contrast, several satellite DNAs have been shown to migrate fast. The structural property that causes the fast migration, however, is not clarified so far on molecular basis. We have investigated the structural property of a satellite DNA, which contains consecutive purine sequences and migrates faster in gel, by CD spectroscopy. Partial formation of an A-form-like structure has been suggested. Reduction in DNA length due to the formation of the A-form-like structure may be responsible for the fast migration. The pronounced rigidity of DNA may also contribute to the behavior.
Matsugami
Akimasa
A
Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, Japan.
Tani
Keiko
K
Ouhashi
Kiyoshi
K
Uesugi
Seiichi
S
Morita
Mitsunori
M
Ohyama
Takashi
T
Katahira
Masato
M
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D013997:Time Factors
D004247:DNA