title: Protoplast fusion between Geotrichum candidium and Phanerochaete chrysosporium to produce fusants for corn stover fermentation.
abstract: Lignin impedes the digestion of corn stover when used as an animal feed. Phanerochaete chrysosporium is an efficient lignindegrader. Geotrichum candidum can be used to produce single-cell protein. In this study, protoplasts of the two fungi were prepared and fused. After screening, one of the fusants, Fusant R1, was selected for corn stover fermentation. It decreased lignin from 109 to 54 g/kg and increased protein from 48 to 67 g/kg in corn stover. Comparison with their parental strains indicated that the fusant obtained the lignin-degrading ability from P. chrysosporium and the protein-accumulating ability from G. candidium.
authors.affiliation: School of Animal Science and Veterinary, Shenyang Agricultural University, Shenyang, China 110161. syndzhy@yahoo.com.cn
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Lin
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Zhu
authors.forename: Chang-Jiang
authors.initials: CJ
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Li
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Wu
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Jing-Hai
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D018533:Biomass; D002459:Cell Fusion; D005285:Fermentation; D005848:Geotrichum / Q000378:metabolism*; D007527:Isoenzymes / Q000032:analysis; D008031:Lignin / Q000378:metabolism; D020075:Phanerochaete / Q000378:metabolism*; D011523:Protoplasts / Q000166:cytology*; D003313:Zea mays / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D008031:Lignin