Ochrobactrum oryzae sp. nov., an endophytic bacterial species isolated from deep-water rice in India.
A non-pigmented, motile, Gram-negative bacterium designated MTCC 4195(T) was isolated from surface-sterilized seeds and plant tissue from deep-water rice (Oryza sativa) cultivated in Suraha Tal Lake in northern India. This isolate was shown to reinfect and colonize deep-water rice endophytically. The highest level of 16S rRNA sequence similarity (96.8 %) to strain MTCC 4195(T) was shown by Ochrobactrum gallinifaecis DSM 15295(T). Strain MTCC 4195(T) utilized gamma-hydroxybutyric acid, adonitol, d-glucosaminic acid and arabinose as carbon sources, but failed to use gentiobiose or citrate. The cell-wall fatty acids of strain MTCC 4195(T) were characterized by the presence of a relatively large proportion of C(18 : 1)omega7c and a relative small proportion of C(16 : 0) in comparison with Ochrobactrum species. DNA-DNA relatedness studies showed less than 52 % binding with the DNAs of type strains of other species of the genus Ochrobactrum. On the basis of phenotypic and genotypic characteristics and the results of 16S rRNA gene sequence analysis, the novel species Ochrobactrum oryzae sp. nov. is proposed, with MTCC 4195(T) (=DSM 17471(T)) as the type strain.
Tripathi
Anil K
AK
School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi - 221005, India.
Verma
Subhash C
SC
School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi - 221005, India.
Chowdhury
Soumitra Paul
SP
School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi - 221005, India.
Lebuhn
Michael
M
Institute of Water Quality Control and Waste Management, Technical University of Munich, Am Coulombwall, 85748 Garching, Germany.
Gattinger
Andreas
A
GSF-National Centre for Environment and Health, Institute of Soil Ecology, Ingolstaedter Landstrasse 1, 85764 Neuherberg, Germany.
Schloter
Michael
M
GSF-National Centre for Environment and Health, Institute of Soil Ecology, Ingolstaedter Landstrasse 1, 85764 Neuherberg, Germany.
D050260:Carbohydrate Metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D020459:Genes, rRNA; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D042041:Ochrobactrum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
D002264:Carboxylic Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S