Phosphorylation of nonmuscle myosin heavy chain IIA on Ser1917 is mediated by protein kinase C beta II and coincides with the onset of stimulated degranulation of RBL-2H3 mast cells.
Dynamic remodeling of the actinomyosin cytoskeleton is integral to many biological processes. It is regulated, in part, by myosin phosphorylation. Nonmuscle myosin H chain IIA is phosphorylated by protein kinase C (PKC) on Ser(1917). Our aim was to determine the PKC isoform specificity of this phosphorylation event and to evaluate its potential role in regulated secretion. Using an Ab against the phosphorylated form of Ser(1917), we show that this site is not phosphorylated in unstimulated RBL-2H3 mast cells. The physiological stimulus, Ag, or the pharmacological activators, PMA plus A23187, induced Ser(1917) phosphorylation with a time course coincident with the onset of granule mediator secretion. Dephosphorylation at this site occurred as Ag-stimulated secretion declined from its peak, but dephosphorylation was delayed in cells activated with PMA plus A23187. Phosphate incorporation was also enhanced by PMA alone and by inhibition of protein phosphatase 2A. Gö6976, an inhibitor of conventional PKC isoforms, abolished secretion and Ser(1917) phosphorylation with similar dose dependencies consistent with involvement of either PKCalpha or PKCbeta. Phorbol ester-stimulated Ser(1917) phosphorylation was reconstituted in HEK-293 cells (which lack endogenous PKCbeta) by overexpression of both wild-type and constitutively active PKCbetaII but not the corresponding PKCbetaI or PKCalpha constructs. A similar selectivity for PKCbetaII overexpression was also observed in MIN6 insulinoma cells infected with recombinant PKC wild-type adenoviruses. Our results implicate PKC-dependent phosphorylation of myosin H chain IIA in the regulation of secretion in mast cells and suggest that Ser(1917) phosphorylation might be a marker of PKCbetaII activation in diverse cell types.
Ludowyke
Russell I
RI
Garvan Institute of Medical Research, St. Vincent's Hospital, Sydney, Australia.
Elgundi
Zehra
Z
Kranenburg
Tanya
T
Stehn
Justine R
JR
Schmitz-Peiffer
Carsten
C
Hughes
William E
WE
Biden
Trevor J
TJ
D000818:Animals; D000918:Antibody Specificity; D015550:Cell Degranulation / Q000276:immunology; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D007106:Immune Sera / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008407:Mast Cells / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D024507:Nonmuscle Myosin Type IIA / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D064546:Protein Kinase C beta; D051381:Rats; D012694:Serine / Q000378:metabolism*; D015854:Up-Regulation / Q000276:immunology
D007106:Immune Sera; D007527:Isoenzymes; D012694:Serine; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D024507:Nonmuscle Myosin Type IIA; D018995:Myosin Heavy Chains