Protein expression is increased by a class III AU-rich element and tethered CUG-BP1.
In mammalian somatic cells, the post-transcriptional control of cytokine or proto-oncogene expression is often achieved by factors binding to sequence elements in the 3' untranslated region (3'UTR). The most studied are the AU-rich elements (ARE) that have been divided into three classes. Here, we show that in mammalian cells, the presence of the class III c-jun ARE in the 3'UTR of a reporter mRNA enhanced reporter protein expression. In contrast, the presence of a class II ARE in the 3'UTR decreased reporter protein expression. CUG-BP1/CELF1 is able to bind c-jun ARE. Protein expression was enhanced similarly to what was observed for c-jun ARE when the reporter mRNA contained a synthetic CUG-BP1/CELF1-binding site, or when this protein was tethered to the 3'UTR of a reporter mRNA. These results reveal an unexpected complexity of ARE-mediated post-transcriptional regulations, and indicate a function for CUG-BP1/CELF1 in class III ARE directed regulations.
Barreau
Carine
C
CNRS UMR6061 Génétique et Développement, Université de Rennes 1, France.
Watrin
Tanguy
T
Beverley Osborne
H
H
Paillard
Luc
L
D020413:3' Untranslated Regions / Q000145:classification / Q000235:genetics*; D000241:Adenosine / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D051379:Mice; D011485:Protein Binding; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D014529:Uridine / Q000235:genetics*
D020413:3' Untranslated Regions; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun; D016601:RNA-Binding Proteins; D000241:Adenosine; D014529:Uridine