[Determination of aflatoxins in hot chilli products by matrix solid-phase dispersion and liquid chromatography].
A new method based on matrix solid-phase dispersion (MSPD) extraction with neutral alumina and co-column purification with graphitized carbon black has been developed to determine aflatoxins B1, B2, G1, G2 in hot chilli products. The method includes liquid chromatography and fluorescence detection with on-line post-column derivatization with bromine. Optimization of different parameters, such as the type of solid supports for matrix dispersion and co-column clean-up was carried out. The recoveries of aflatoxins B1, B2, G1 and G2 were 95.4%, 87.3%, 91.5% and 92.6%, respectively, with relative standard deviations ranging from 3.3% to 6.1%. The limits of detection were in the range of 0.10 ng/g (B2, G2) to 0.25 ng/g (B1, G1). In addition, the comparison of the extraction and purification effect of MSPD with immunity affinity column showed that, MSPD is a valid method to analyze aflatoxins in hot chilli products.
Zheng
Ping
P
Anhui Entry-Exit Inspection and Quarantine Bureau, Hefei 230061, China. ahciq_hx@163.com
Sheng
Xuan
X
Yu
Xiaofeng
X
Hu
Yanyun
Y
D016604:Aflatoxin B1 / Q000032:analysis; D000348:Aflatoxins / Q000032:analysis* / Q000145:classification; D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D057230:Limit of Detection; D010936:Plant Extracts / Q000737:chemistry*; D012015:Reference Standards; D052616:Solid Phase Extraction / Q000379:methods; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D016604:Aflatoxin B1