title: Crystallization and preliminary X-ray analysis of Sau3AI C-terminal 232-419 amino acids fragment.
abstract: The C-terminal 232-419 amino acids fragment of endonuclease Sau3AI has been successfully expressed in Escherichia coli with 6 His at its N-terminal. After purification and crystallization, one completed 2.8 A data set was collected using a Rigaku R-AXIS IV++ diffractometer. The plate-like crystals belong to orthorhombic space group P2(1)2(1)2(1) with the cell dimension of a = 34.75, b = 76.82, c = 123.59A and contain one molecule per asymmetric unit.
authors.affiliation: Shanghai Institutes of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
authors.forename: Jiaping
authors.initials: J
authors.lastname: Song
authors.forename: Chunyan
authors.initials: C
authors.lastname: Xu
authors.forename: Yu
authors.initials: Y
authors.lastname: Ding
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Hu
authors.forename: Jianhua
authors.initials: J
authors.lastname: He
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases