title: Advances in immunoproteomics for serological characterization of microbial antigens.
abstract: We propose a multi-dimensional strategy, associating immunodetection to a protein fractionating two-dimensional liquid chromatography tool, for serological characterization of microbial antigens. The originality of such immunoproteomic approaches resides in their application in large-scale studies for rapid serotyping of micro-organisms, evaluation of immunomes and could be proposed in the development and monitoring of vaccines.
authors.affiliation: Center of Research on Prion Proteins, Institute of Pharmacy-CHU, B36, Avenue de l'Hôpital 1, B-4000 University of Liège, Belgium.
authors.forename: Nandini
authors.initials: N
authors.lastname: Falisse-Poirrier
authors.forename: Virginie
authors.initials: V
authors.lastname: Ruelle
authors.forename: Benaissa
authors.initials: B
authors.lastname: ElMoualij
authors.forename: Danièle
authors.initials: D
authors.lastname: Zorzi
authors.forename: Olivier
authors.initials: O
authors.lastname: Pierard
authors.forename: Ernst
authors.initials: E
authors.lastname: Heinen
authors.forename: Edwin
authors.initials: E
authors.lastname: De Pauw
authors.forename: Willy
authors.initials: W
authors.lastname: Zorzi
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology*; D001412:Bacillus subtilis / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology*; D015153:Blotting, Western; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D008853:Microscopy / Q000379:methods; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D012703:Serotyping; D013057:Spectrum Analysis; D053719:Tandem Mass Spectrometry
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D020543:Proteome