title: Wnt signaling and a Hox protein cooperatively regulate psa-3/Meis to determine daughter cell fate after asymmetric cell division in C. elegans.
abstract: Asymmetric cell division is a mechanism for achieving cellular diversity. In C. elegans, many asymmetric cell divisions are controlled by the Wnt-MAPK pathway through POP-1/TCF. It is poorly understood, however, how POP-1 determines the specific fates of daughter cells. We found that nob-1/Hox, ceh-20/Pbx, and a Meis-related gene, psa-3, are required for asymmetric division of the T hypodermal cell. psa-3 expression was asymmetric between the T cell daughters, and it was regulated by POP-1 through a POP-1 binding site in the psa-3 gene. psa-3 expression was also regulated by NOB-1 and CEH-20 through a NOB-1 binding sequence in a psa-3 intron. PSA-3 can bind CEH-20 and function after the T cell division to promote the proper fate of the daughter cell. These results indicate that cooperation between Wnt signaling and a Hox protein functions to determine the specific fate of a daughter cell.
authors.affiliation: Laboratory for Cell Fate Decision, RIKEN, Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Arata
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kouike
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Michael A
authors.initials: MA
authors.lastname: Herman
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sawa
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001665:Binding Sites / Q000235:genetics; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002455:Cell Division; D017238:Genes, Helminth; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D007438:Introns; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D029742:Caenorhabditis elegans Proteins; D018398:Homeodomain Proteins; D014157:Transcription Factors; D051153:Wnt Proteins; C418523:ceh-20 protein, C elegans; D049452:Green Fluorescent Proteins