title: Posttransplant lymphoproliferative disorder in a kidney-pancreas transplanted recipient: simultaneous development of clonal lymphoid B-cell proliferation of host and donor origin.
abstract: Posttransplant lymphoproliferative disorders (PTLDs) are lymphoid proliferations or lymphomas that develop as a consequence of immunosuppression after solid organ or bone marrow transplantation and are mostly associated with an Epstein-Barr virus infection. The morphologic categories include different types of benign and malignant lymphoid proliferations. The majority of PTLDs is of B-cell origin with clonal rearrangements of the immunoglobulin genes. The PTLDs in solid organ transplants are reported to be either of host or of donor origin. Donor-related PTLDs frequently involve the allograft. We report a case of a 52-year-old woman recipient who developed simultaneously PTLDs in several organs 5 month after receiving a sex-mismatched renal and pancreas allograft. Immunosuppression regimen comprised antithymocyte globulin, tacrolimus, mycophenolate mofetil, and steroids. Pathologic features appeared as polymorphic PTLDs in the renal allograft, liver, and central nervous system (CNS). Molecular genetic studies revealed different clonal immunoglobulin heavy chain gene rearrangements in all 3 organs as determined by polymerase chain reaction (PCR). Epstein-Barr virus were detected by nested PCR and in situ hybridization in all 3 tumors. The PTLDs in liver and CNS were of host origin whereas the allograft kidney PTLD was found to originate from the male donor as shown by the simultaneous detection of female and male sex chromosomes by PCR and fluorescence in situ hybridization. The recipient died in consequence of the CNS involvement, after intracerebral hemorrhage with uncal and tonsillar herniation.
authors.affiliation: Department of Pathology, Klinikum Darmstadt, Grafenstrasse 9, 64276 Darmstadt, Germany. pathologie-heyny@klinikum-darmstadt.de
authors.forename: Roland
authors.initials: R
authors.lastname: Heyny-von Haussen
authors.forename: Karin
authors.initials: K
authors.lastname: Klingel
authors.forename: Werner
authors.initials: W
authors.lastname: Riegel
authors.forename: Reinhard
authors.initials: R
authors.lastname: Kandolf
authors.forename: Gerhard
authors.initials: G
authors.lastname: Mall
mesh_terms: D001402:B-Lymphocytes / Q000473:pathology*; D002490:Central Nervous System / Q000473:pathology; D041321:Chromosomes, Human, X / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics; D002999:Clone Cells; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000473:pathology; D017809:Fatal Outcome; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016867:Immunocompromised Host; D017404:In Situ Hybridization, Fluorescence; D007668:Kidney / Q000473:pathology; D016030:Kidney Transplantation / Q000009:adverse effects* / Q000473:pathology; D008099:Liver / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000209:etiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016035:Pancreas Transplantation / Q000009:adverse effects* / Q000473:pathology; D011183:Postoperative Complications; D012737:Sex Factors; D014019:Tissue Donors