Genetic association of blaSHV-5 with transposable elements IS26 and IS5 in Klebsiella pneumoniae from Taiwan.
A cloned 5,248-bp EcoRI fragment from the Klebsiella pneumoniae transferable plasmid pKP53 (> 70 kb) containing bla(SHV-5) was sequenced. Insertion sequences IS26 and IS5 were found downstream from bla(SHV-5). The DNA sequences of the genetic environment surrounding bla(SHV-5) were homologous to plasmid p1658/97 from Escherichia coli, containing a truncated recF gene and a truncated deoR gene upstream and downstream from bla(SHV-5), respectively. RecF may be involved in bla(SHV-5) translocation to the plasmid by RecF-dependent recombination. This novel genetic environment may be associated with the successful proliferation and/or expression of SHV-5 in K. pneumoniae strains from Taiwan.
Yu
W L
WL
Department of Critical Care Medicine, Chi-Mei Medical Center, Tainan, Taiwan.
Chen
S C
SC
Hung
S W
SW
Chuang
Y C
YC
Chung
J G
JG
Chen
I C
IC
Wu
L T
LT
D001483:Base Sequence; D004251:DNA Transposable Elements; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
D004251:DNA Transposable Elements; C117193:beta-lactamase SHV-5; D001618:beta-Lactamases