Protective immune responses against systemic candidiasis mediated by phage-displayed specific epitope of Candida albicans heat shock protein 90 in C57BL/6J mice.
Specific epitope (DEPAGE) of Candida albicans heat shock protein 90 (SE-CA-HSP90) was successfully expressed on the surface of filamentous phage fd, fused to the major coat protein pVIII. Protective immune responses mediated by hybrid-phage expressing SE-CA-HSP90 in C57BL/6J mice were evaluated in this paper. The results showed that hybrid-phage particles induced the specific antibody response against SE-CA-HSP90, enhanced delayed-type hypersensitivity (DTH) response, natural killer (NK) cell activity and concanavalin A (ConA)-induced splenocyte proliferation. Furthermore, this study demonstrated that hybrid-phage-immunized mice had fewer colony forming unites (CFU) in the kidneys compared with wild-type phage-immunized mice and TE (1.0mM EDTA, 0.01M Tris-HCl, pH 8.0)-injected mice and showed statistically significant survival advantage over TE-injected group. In conclusion, the results suggest that the hybrid-phage displaying SE-CA-HSP90 may be considered as a potential candidate for producing vaccines against systemic candidiasis.
Wang
Guiyun
G
School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun City, Jilin Province 130024, People's Republic of China.
Sun
Meiyan
M
Fang
Jinbo
J
Yang
Qiong
Q
Tong
Haibin
H
Wang
Li
L
D000818:Animals; D000918:Antibody Specificity; D017104:Bacteriophage M13 / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002176:Candida albicans / Q000235:genetics / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology / Q000517:prevention & control*; D049109:Cell Proliferation; D003208:Concanavalin A; D018985:Epitopes, B-Lymphocyte / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular; D007694:Killer Cells, Natural / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology
D018985:Epitopes, B-Lymphocyte; D018841:HSP90 Heat-Shock Proteins; D003208:Concanavalin A