Adult human bone marrow stromal spheres express neuronal traits in vitro and in a rat model of Parkinson's disease.
Adult human bone marrow stromal cells (hMSCs) grown in suspension culture gave rise to spheres of neural progenitor (NP) cells, capable of expressing both dopaminergic (DA) and GABAergic (GABA) traits. After transplantation into the Parkinsonian rat, human NPs and neurons were present at 2 weeks. Although no DA neurons appeared to survive transplantation, there were abundant GABA neurons present in the graft. By 4 weeks, however, all cells had died. Finding ways to prolong survival and promote the appropriate neurotransmitter phenotype is essential if hMSCs are to be clinically useful.
Suon
Sokreine
S
Department of Molecular Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA; Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University, 900 Walnut Street, JHN 462, Philadelphia, PA 19107, USA.
Yang
Ming
M
Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University, 900 Walnut Street, JHN 462, Philadelphia, PA 19107, USA.
Iacovitti
Lorraine
L
Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University, 900 Walnut Street, JHN 462, Philadelphia, PA 19107, USA. Electronic address: lorraine.iacovitti@jefferson.edu.
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods*; D016923:Cell Death / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D006085:Graft Survival / Q000187:drug effects / Q000502:physiology; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D020734:Parkinsonian Disorders / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D051381:Rats; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000637:transplantation*; D017211:Treatment Failure; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D015415:Biomarkers; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D005680:gamma-Aminobutyric Acid; D004298:Dopamine