Mapping protein-protein interactions by mass spectrometry.
Mass spectrometry is currently at the forefront of technologies for mapping protein-protein interactions, as it is a highly sensitive technique that enables the rapid identification of proteins from a variety of biological samples. When used in combination with affinity purification and/or chemical cross-linking, whole or targeted protein interaction networks can be elucidated. Several methods have recently been introduced that display increased specificity and a reduced occurrence of false-positives. In the future, information gained from human protein interaction studies could lead to the discovery of novel pathway associations and therapeutic targets.
Vasilescu
Julian
J
The Ottawa Institute of Systems Biology, Ottawa, ON, Canada.
Figeys
Daniel
D
D000818:Animals; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011485:Protein Binding / Q000502:physiology; D025941:Protein Interaction Mapping / Q000379:methods*; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000502:physiology
D003432:Cross-Linking Reagents; D020543:Proteome