[Study on the interaction of lomefloxacin terbium complex and bovine serum albumin by fluorescence spectra].
Using lomefloxacin-terbium as a fluorescent probe, the interaction of LFLX-Tb3+ and bovine serum albumin (BSA) was investigated by fluorescence spectra. The interaction of LFLX and BSA is quite strong. LFLX can affect the conformation of BSA to some degree. Meanwhile, the electrostatic binding of the BSA with Tb3+ in LFLX-Tb3+ complex could decease the nonradiative energy loss through O-H vibration of H2O molecule in Tb3+ complex. Under the optimum conditions, BSA can enhance the fluorescence intensity of the lomefloxacin-terbium complex at 545 nm, and the enhanced fluorescence intensity of Tb3+ is proportional to the concentration of BSA. Based on that, a new method is constructed for the determination of BSA. The linear ranges for BSA are 16.5-148.5 microg x mL(-1) with a detection limit of 68.8 ng x mL(-1) and relative standard deviations of 1.4%. This method is simple, practical and relatively free of interference from coexistent substances.
He
Hua
H
China Pharmaceutical University, Nanjing 210009, China.
Ye
Hai-ying
HY
Dai
Li
L
Jiao
Qing-cai
QC
Chuong
Pham-huy
PH
D000818:Animals; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry*; D024841:Fluoroquinolones / Q000737:chemistry*; D057230:Limit of Detection; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013725:Terbium / Q000737:chemistry*
D005456:Fluorescent Dyes; D024841:Fluoroquinolones; D013725:Terbium; D012710:Serum Albumin, Bovine; C053091:lomefloxacin