[Study on purification of 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside from Polygonum multiflori].
OBJECTIVE
To study the conditions and parameters of purifying 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside from Polygonum multiflori.


METHODS
Absorption capacity of four resins for 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside was compared. With the adsorption ability as indexes, the process of absorbing and purifying 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside from P. multiflori with S-8 macroporous resin absorbent was selected by orthogonal design.


RESULTS
The S-8 resin was the best of the four resins. The optimum process condition was 50% ethanol as eluting solvent, the flow rate at 1.5 mL x min(-1), pH at 7-8, and the solution concentration at 0.2 g x mL(-1). The absorption capacity by this process was 36.89 mg x g(-1).


CONCLUSIONS
The process is simple and convenient and the regeneration of resin is easy, so this method of purification is advisable.
Jiang
Tao-tao
TT
School of Biological and Environmental Engineering, Jiangsu University, Zhenjiang, China.
Ouyang
Zhen
Z
Miao
Ya-dong
YD
Zhang
Xiao-feng
XF
D000042:Absorption; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification*
D006027:Glycosides; D010936:Plant Extracts; D012117:Resins, Synthetic; D013267:Stilbenes