Purification and characterization of two types of chitosanase from a Microbacterium sp.
Two extracellular chitosanases (ChiX and ChiN) were extracted from Microbacterium sp. OU01 with Mr values of 81 kDa (ChiX) and 30 kDa (ChiN). ChiN was optimally active at pH 6.2 and 50 degrees C and ChiX at pH 6.6 and 60 degrees C (assayed over 15 min). Both the activities increased with the degree of deacetylation (DDA) of chitosan. ChiN hydrolyzed oligomers of glucosamine (GlcN) larger than chitopentaose, and chitosan with 62-100% DDA; but ChiX acted on chitosan and released GlcN. Hydrolysis of chitosan with 99% DDA by ChiN released chitobiose, chitotriose and chitotetraose as the major products.
Sun
Yuying
Y
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P.R China.
Liu
Wanshun
W
Han
Baoqin
B
Zhang
Jiquan
J
Liu
Bing
B
D000192:Actinomycetales / Q000201:enzymology*; D000596:Amino Acids / Q000032:analysis; D006026:Glycoside Hydrolases / Q000032:analysis / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D021241:Spectrometry, Mass, Electrospray Ionization; D013379:Substrate Specificity; D013696:Temperature; D014783:Viscosity
D000596:Amino Acids; D006026:Glycoside Hydrolases; C019868:chitosanase