[Development and application of thiophilic adsorption chromatography for antibody purification].
Thiophilic adsorption chromatography binds antibodies and other proteins in the presence of high concentration of salt. When eluted by decreasing the salt concentration, the high purity and recovery of proteins can be achieved. It is a new chromatographic technology for the purification of proteins. The review introduces the method for the purification of antibodies and covers the development and application of the original technology.
Liu
Xuan
X
Life Science and Engineering College, Shaanxi University of Science and Technology, Xianyang 712081, China.
Song
Hongxin
H
D000327:Adsorption; D000485:Allergens / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002845:Chromatography / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013045:Species Specificity
D000485:Allergens; D000906:Antibodies; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007136:Immunoglobulins; D012965:Sodium Chloride