Evaluation of the C6 enzyme-linked immunoadsorbent assay for the serodiagnosis of Lyme borreliosis in north-eastern Italy.
A novel antigen preparation--the synthetic C6 peptide, a conserved portion of the variable VlsE antigens of Borrelia burgdorferi--has been evaluated for serodiagnosis of Lyme borreliosis (LB) by an ELISA procedure. Serum specimens were from early and late LB patients, all resident in an endemic area in north-eastern Italy. The specificity of the test approached the 100% and sensitivity was in the order of 63% (early LB) and 100% (late LB); this performance is superior to the preceding generation of Lyme disease tests.
Cinco
Marina
M
Laboratorio Spirochete, Dipartimento di Scienze Biomediche, Università di Trieste, Italy. cinco@dsbmail.units.it
Murgia
Rossella
R
D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D025065:Borrelia burgdorferi / Q000276:immunology*; D019353:Endemic Diseases; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007558:Italy / Q000453:epidemiology; D008074:Lipoproteins / Q000737:chemistry; D008193:Lyme Disease / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008074:Lipoproteins; C105546:VlsE protein, Borrelia burgdorferi