Recommended method for recovery of Toxocara and other geohelminth eggs from soil.
The flotation method elaborated for recovery of Toxocara and other geohelminth eggs from soil is described. Soil samples of about 500 ml volume are picked from 3-cm superficial layer of the ground. In the laboratory, 40 g of dry and sifted material is analysed according to following procedure: 1 h standing, 20 minutes shaking and 3 minutes centrifugation (1500 rpm) in 5% sodium hydroxide (NaOH), then centrifugation, like above, with H2O for washing the sample and next with the saturated sodium nitrate (NaNO3) for flotation the eggs. Specimen is prepared by placing a cover slip on the positive meniscus of the flotation liquid.
Mizgajska-Wiktor
Hanna
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Department of Biology and Environmental Protection, Eugeniusz Piasecki University School of Physical Education, Królowej Jadwigi 27/39, 61-871 Poznań. mizgajska@awf.poznan.pl
D000818:Animals; D004463:Ecology / Q000379:methods; D010063:Ovum; D012987:Soil / Q000032:analysis* / Q000469:parasitology*; D013048:Specimen Handling; D014119:Toxocara / Q000302:isolation & purification*
D012987:Soil