A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.
Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.
Goldberg
Susanne M D
SM
J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA. sgoldberg@venterinstitute.org
Johnson
Justin
J
Busam
Dana
D
Feldblyum
Tamara
T
Ferriera
Steve
S
Friedman
Robert
R
Halpern
Aaron
A
Khouri
Hoda
H
Kravitz
Saul A
SA
Lauro
Federico M
FM
Li
Kelvin
K
Rogers
Yu-Hui
YH
Strausberg
Robert
R
Sutton
Granger
G
Tallon
Luke
L
Thomas
Torsten
T
Venter
Eli
E
Frazier
Marvin
M
Venter
J Craig
JC
D001709:Biotechnology / Q000379:methods* / Q000639:trends; D019295:Computational Biology / Q000379:methods; D020451:Contig Mapping; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D017422:Sequence Analysis, DNA / Q000379:methods*