Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs.
The migration of B cells into secondary lymphoid organs is required for the generation of an effective immune response. Here we analyzed the involvement of SWAP-70, a Rac-interacting protein involved in actin rearrangement, in B cell entry into lymph nodes. We noted reduced migration of Swap70-/- B cells into lymph nodes in vivo. Swap70-/- B cells rolled and adhered, yet accumulated in lymph node high endothelial venules. This defect was not due to impaired integrin expression or chemotaxis. Instead, Swap70-/- B cells aberrantly regulated integrin-mediated adhesion. During attachment, Swap70-/- B cells showed defective polarization and did not form uropods or stabilize lamellipodia at a defined region. Thus, SWAP-70 selectively regulates processes essential for B cell entry into lymph nodes.
Pearce
Glen
G
Institute of Physiological Chemistry, Dresden University of Technology, 01307 Dresden, Germany.
Angeli
Veronique
V
Randolph
Gwendalyn J
GJ
Junt
Tobias
T
von Andrian
Ulrich
U
Schnittler
Hans-Joachim
HJ
Jessberger
Rolf
R
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology; D004268:DNA-Binding Proteins / Q000276:immunology*; D020662:Guanine Nucleotide Exchange Factors / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins / Q000276:immunology*; D016201:Receptors, Lymphocyte Homing / Q000276:immunology*
D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins; D016201:Receptors, Lymphocyte Homing; C113365:Swap70 protein, mouse