title: Suppression of IFN-gamma production in atopic group at the acute phase of RSV infection.
abstract: Several studies have suggested that respiratory syncytial virus (RSV) bronchiolitis induced the change of cytokine production profile in childhood. We sought to determine whether the RSV-induced cytokine production was affected by the patient's atopic background. We quantified interferon-gamma (IFN-gamma) and interleukin (IL)-4 in the supernatant of peripheral blood mononuclear cells (PBMCs) cultured for 24 h and in the presence of phytohemaglutinin (PHA), IL-12, or IL-18, from 14 infants who were divided into two groups, those who are non-atopic and an atopic group. In RSV-infected infants with atopic diseases, IFN-gamma production from IL-12- or especially IL-18-stimulated PBMCs was subtotally suppressed in the acute phase, whereas in RSV-infected infants without atopic diseases IFN-gamma production was not suppressed on acute phase. The IFN-gamma suppression observed in the atopic group is not caused by the immaturity of an infant's immune system since reduced IFN-gamma production to RSV is not observed in the infants of non-atopic group. IFN-gamma suppression in regard to RSV infection might be caused by some genetic factor involved in the development of atopic disease such as IL-18 signal cascade.
authors.affiliation: Department of Pediatrics, Graduate School of Medicine, Gifu University, Yanagido, Gifu, Japan. hideo@cc.gifu-u.ac.jp
authors.forename: Hideo
authors.initials: H
authors.lastname: Kaneko
authors.forename: Eiko
authors.initials: E
authors.lastname: Matsui
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Asano
authors.forename: Zenichiro
authors.initials: Z
authors.lastname: Kato
authors.forename: Takahide
authors.initials: T
authors.lastname: Teramoto
authors.forename: Minako
authors.initials: M
authors.lastname: Aoki
authors.forename: Norio
authors.initials: N
authors.lastname: Kawamoto
authors.forename: Li Ai
authors.initials: LA
authors.lastname: Lian
authors.forename: Kimiko
authors.initials: K
authors.lastname: Kasahara
authors.forename: Naomi
authors.initials: N
authors.lastname: Kondo
mesh_terms: D000210:Acute-Phase Reaction / Q000276:immunology / Q000821:virology*; D002675:Child, Preschool; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000821:virology; D007223:Infant; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D020382:Interleukin-18 / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*
chemical_list: D020382:Interleukin-18; D018664:Interleukin-12; D007371:Interferon-gamma