title: Failure of additional ketoanalogues of branched chain amino acids to improve the response to parenteral nutrition after experimental trauma in the rat.
abstract: The ketoanalogues of branched chain amino acids (KAA) may improve the post-traumatic protein metabolism. We studied the effects of additional KAA, supplied as a part of total parenteral nutrition (TPN), on liver protein synthesis and nitrogen balance in rats after standard surgical trauma. A TPN-regimen, containing 36 mmol/kg/day KAA and 58 mmol/kg/day amino acids was compared to an isonitrogenous (1.0 gN/kg/day) TPN-regimen containing 58 mmol/kg/day amino acids and no KAA in a 48 h experiment. The liver protein synthesis measured by perfusion with C14-leucine in vitro was similar in both groups 2 days after surgery (59.2 +/- 16.2 vs. 64.3 +/- 15.6 arbitrary units, mean +/- SD). The nitrogen balances were negative and of similar magnitude (-0.66 +/- 0.36 vs. -0.17 +/- 0.45 gN/kg/48 h, mean +/- SD). We conclude that TPN supplemented by high supply of KAA offers no systematic advantage over TPN alone after experimental surgical trauma.
authors.affiliation: Department of Anaesthesiology, Turku University Central Hospital, 20520 Turku, Finland.
authors.forename: O
authors.initials: O
authors.lastname: Kirvelä
authors.forename: J
authors.initials: J
authors.lastname: Takala