Plant tissue-based chemiluminescence biosensor for ethanol.
A plant tissue-based chemiluminescence biosensor for ethanol based on using mushroom (Agaricus bisporus) tissue as the recognition element is proposed in this paper. The principle for ethanol sensing relies on the luminol-potassium hexacyanoferrate(III)-hydrogen peroxide transducer reaction, in which hydrogen peroxide is produced from the ethanol enzymatic catalytic oxidation by oxygen under the catalysis of alcohol oxidase in the tissue column. Under optimum conditions, the method allowed the measurement of ethanol in the range of 0.001 - 2 mmol/l with a detection limit (3 sigma) of 0.2 micromol/l. The relative standard deviation (RSD) was 4.14% (n = 11) for 0.05 mmol/l ethanol. The proposed method has been applied to the determination of ethanol in biological fluids and beverages with satisfactory results.
Huang
Yuming
Y
College of Chemistry and Chemical Engineering, Chongqing Key Laboratory of Modern Analytical Sciences, Ministry of Education, Southwest University, Beibei, Chongqing 400715, P.R. China. yuminghuang2000@yahoo.com
Wu
Fangqiong
F
D000364:Agaricus / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D000431:Ethanol / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D013696:Temperature
D000431:Ethanol