[Comparative study on GOD-trinder method for measuring glucose in serum with different benzene-original phenols].
Comparative studies were carried out on GOD-trinder method for measuring glucose in serum with four different kinds of benzene-original phenols-phenyl hydroxide, 2,4-dichlorophenol, 2,6-dichlorophenol and resorcinol. It is shown that when the three coloring systems-phenyl hydroxide, 2,4-dichlorophenol or resorcinol coupling with glucose oxidase-peroxidase-4-a minoantipyrine-hydrogen peroxide (GOD-POD-4-AA-H2O2) respectively were used for clinical assay of glucose in serum, the determination results showed no significant differences (P > 0.05). Compared with phenyl hydroxide, the coloring system of 2,4-dichlorophenol is better in the aspects of sensitivity, precision and accuracy; the resorcinol system has lower sensitivity with the advantage of faster detecting velocity, and larger linear range, and can be used for on-line rapid analysis of samples with more concentrated glucose in industry.
Xu
Sheng
S
College of Chemistry and Chemical Engineering, Nanhua University, Hengyang 421001, China.
Deng
Jian
J
Xu
Jin-sheng
JS
Yuan
Ya-li
YL
Zhang
Wei
W
D001786:Blood Glucose / Q000032:analysis*; D002733:Chlorophenols / Q000737:chemistry*; D057075:Enzyme Assays / Q000379:methods*; D005949:Glucose Oxidase / Q000737:chemistry*; D010544:Peroxidases / Q000737:chemistry*
D001786:Blood Glucose; D002733:Chlorophenols; D005949:Glucose Oxidase; D010544:Peroxidases; C023057:glucose peroxidase; C032729:2,6-dichlorophenol; C004762:2,4-dichlorophenol