Comparison of Campylobacter jejuni PFGE and Penner subtypes in human infections and in water samples from the Taieri River catchment of New Zealand.
OBJECTIVE
To determine the degree of overlap in strain types of Campylobacter jejuni isolated from clinical cases and water samples from the Taieri catchment in the South Island of New Zealand.


RESULTS
Thermophilic Campylobacter were collected from human cases of infection, the main stem of the Taieri River and streams within distinct land-use types over a 1-year period. Campylobacter jejuni (187 isolates) and Campylobacter lari (four isolates) were identified using a multiplex polymerase chain reaction protocol. Isolates were typed by the Penner method and pulsed-field gel electrophoresis (PFGE) utilizing two restriction endonucleases. Several serotypes and PFGE types occurred in both water samples and clinical cases when the restriction profiles for each enzyme were considered separately. However, when PFGE profiles and serotyping were combined, there was no overlap between Camp. jejuni types from water and clinical cases.


CONCLUSIONS
The results of this study indicate that recreational water in the Taieri catchment is not a major source of campylobacteriosis in the Dunedin area.


CONCLUSIONS
This study suggests the risk of acquiring campylobacteriosis from surface waters in the Taieri catchment is considerably lower than previously predicted and highlights the necessity of using two endonucleases in PFGE typing.
Eyles
R F
RF
Ecology and Health Research Centre, Department of Public Health, Wellington School of Medicine, University of Otago, Wellington, New Zealand.
Brooks
H J L
HJ
Townsend
C R
CR
Burtenshaw
G A
GA
Heng
N C K
NC
Jack
R W
RW
Weinstein
P
P
D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000382:microbiology / Q000635:transmission*; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D004197:Disease Reservoirs; D016521:Electrophoresis, Gel, Pulsed-Field; D005618:Fresh Water; D006801:Humans; D009520:New Zealand; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D014871:Water Microbiology