Tissue engineering of heart valves in vivo using bone marrow-derived cells.
In this study, we tissue-engineered heart valves in vivo using autologous bone marrow-derived cells (BMCs). Canine BMCs were differentiated into endothelial cell (EC)-like cells and myofibroblast (MF)-like cells. Decellularized porcine pulmonary valves were seeded with BMCs and implanted to abdominal aorta and pulmonary valve of bone marrow donor dogs. Histological examination of the explants identified the regeneration of valvular structures expressing CD31 and smooth muscle alpha-actin, indicating the presence of EC-like and MF-like cells in the grafts at 3 and 1 week, respectively, after implantation. Fluorescent microscopic examinations identified the presence of fluorescently labeled cells in the explants, indicating that the implanted BMCs survived and participated in the heart valve reconstitution. This study reports, for the first time, on tissue engineering of heart valve in vivo using BMCs.
Kim
Sang-Soo
SS
Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul, Korea.
Lim
Sang-Hyun
SH
Hong
Yoo-Sun
YS
Cho
Seung-Woo
SW
Ryu
Ju Hee
JH
Chang
Byung-Chul
BC
Choi
Cha Yong
CY
Kim
Byung-Soo
BS
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D004285:Dogs; D006351:Heart Valves; D020007:Implants, Experimental; D023822:Tissue Engineering / Q000379:methods*