Sugarcane molasses and yeast powder used in the Fructooligosaccharides production by Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611.
Different concentrations of sucrose (3-25% w/v) and peptone (2-5% w/v) were studied in the formulation of media during the cultivation of Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611. Moreover, cane molasses (3.5-17.5% w/v total sugar) and yeast powder (1.5-5% w/v) were used as alternative nutrients for both strains' cultivation. These media were formulated for analysis of cellular growth, beta-Fructosyltransferase and Fructooligosaccharides (FOS) production. Transfructosylating activity (U ( t )) and FOS production were analyzed by HPLC. The highest enzyme production by both the strains was 3% (w/v) sucrose and 3% (w/v) peptone, or 3.5% (w/v) total sugars present in cane molasses and 1.5% (w/v) yeast powder. Cane molasses and yeast powder were as good as sucrose and peptone in the enzyme and FOS (around 60% w/w) production by studied strains.
Dorta
Claudia
C
Departamento de Ciências Biológicas-F.C.L., Universidade Estadual Paulista-UNESP, Assis, SP, Av. Dom Antônio, 2100, São Paulo, Brazil. claudorta2000@yahoo.com.br
Cruz
Rubens
R
de Oliva-Neto
Pedro
P
Moura
Danilo José Camargo
DJ
D001230:Aspergillus / Q000254:growth & development* / Q000378:metabolism; D001234:Aspergillus niger / Q000254:growth & development* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D005632:Fructose / Q000032:analysis / Q000096:biosynthesis*; D006602:Hexosyltransferases / Q000032:analysis / Q000096:biosynthesis; D007218:Industrial Microbiology / Q000379:methods*; D008965:Molasses; D009844:Oligosaccharides / Q000032:analysis / Q000096:biosynthesis*; D010461:Peptones / Q000032:analysis; D011208:Powders; D031786:Saccharum / Q000737:chemistry*; D013395:Sucrose / Q000032:analysis; D015003:Yeasts / Q000737:chemistry; D001426:Bacterial Proteins
D003470:Culture Media; D005632:Fructose; D006602:Hexosyltransferases; D009844:Oligosaccharides; D010461:Peptones; D011208:Powders; D013395:Sucrose; C021451:inulosucrase; D001426:Bacterial Proteins