Hydrothermal decomposition of yeast cells for production of proteins and amino acids.
This study examines hydrothermal decomposition of Baker's yeast cells, used as a model for spent Brewer's yeast waste, into protein and amino acids. The reaction was carried out in a closed batch reactor at various temperatures between 100 and 250 degrees C. The reaction products were separated into water-soluble and solid residue. The results demonstrated that the amount of yeast residue decreased with increasing hydrolysis temperature. After 20 min reaction in water at 250 degrees C, 78% of yeast was decomposed. The highest amount of protein produced was also obtained at this condition and was found to be 0.16 mg/mg dry yeast. The highest amount of amino acids (0.063 mg/mg dry yeast) was found at the lowest temperature tested after 15 min. The hydrolysis product obtained at 200 degrees C was tested as a nutrient source for yeast growth. The growth of yeast cells in the culture medium containing 2 w/v% of this product was comparable to that of the cells grown in the medium containing commercial yeast extract at the same concentration. These results demonstrated the feasibility of using subcritical water to potentially decompose proteinaceous waste such as spent Brewer's yeast while recovering more useful products.
Lamoolphak
Wiwat
W
Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Patumwan, Payathai Road, Bangkok 10330, Thailand.
Goto
Motonobu
M
Sasaki
Mitsuru
M
Suphantharika
Manop
M
Muangnapoh
Chirakarn
C
Prommuag
Chattip
C
Shotipruk
Artiwan
A
D000596:Amino Acids / Q000096:biosynthesis*; D007220:Industrial Waste; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis*; D012995:Solubility; D013696:Temperature; D014867:Water / Q000378:metabolism*
D000596:Amino Acids; D007220:Industrial Waste; D029701:Saccharomyces cerevisiae Proteins; D014867:Water