title: Fractal binding and dissociation kinetics of heart-related compounds on biosensor surfaces.
abstract: A fractal analysis is presented for the binding and dissociation of different heart-related compounds in solution to receptors immobilized on biosensor surfaces. The data analyzed include LCAT (lecithin cholesterol acyl transferase) concentrations in solution to egg white apoA-I rHDL immobilized on a biosensor chip surface (1), native, mildly oxidized, and strongly oxidized LDL in solution to a heparin-modified Au-surface of a surface plasmon resonance (SPR) biosensor (2), and TRITC-labeled HDL in solution to a bare optical fiber surface (3). Single-and dual-fractal models were used to fit the data. Values of the binding and the dissociation rate coefficient(s), affinity values, and the fractal dimensions were obtained from the regression analysis provided by Corel Quattro Pro 8.0 (4). The binding rate coefficients are quite sensitive to the degree of heterogeneity on the sensor chip surface. Predictive equations are developed for the binding rate coefficient as a function of the degree of heterogeneity present on the sensor chip surface and on the LCAT concentration in solution and for the affinity as a function of the ratio of fractal dimensions present in the binding and the dissociation phases. The analysis presented provided physical insights into these analyte-receptor reactions occurring on different biosensor surfaces.
authors.affiliation: Chemical Engineering Department, University of Mississippi, University, Mississippi 38677-184, USA.
authors.forename: Atul M
authors.initials: AM
authors.lastname: Doke
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Mathur
authors.forename: Ajit
authors.initials: A
authors.lastname: Sadana
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D003627:Data Interpretation, Statistical; D004529:Egg White; D004563:Electrochemistry / Q000379:methods; D017709:Fractals; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000096:biosynthesis; D011485:Protein Binding; D012984:Software; D020349:Surface Plasmon Resonance; D013997:Time Factors
chemical_list: D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D010100:Oxygen