title: Bacillus massiliensis sp. nov., isolated from cerebrospinal fluid.
abstract: An unidentified Gram-negative-staining, aerobic, rod-shaped, spore-forming bacterium was isolated from a sample of cerebrospinal fluid. Based on comparative analysis of 16S rRNA gene sequences and phenotypic characteristics, the novel isolate was included in the Bacillus sphaericus-like group. The isolate was closely related to Bacillus odysseyi and Bacillus silvestris, with 96.2 and 94.4 % 16S rRNA gene sequence similarity, respectively. The major fatty acid was iso-C(15 : 0) (48 %). The name Bacillus massiliensis sp. nov. is proposed for the novel isolate, with strain 4400831(T) (=CIP 108446(T)=CCUG 49529(T)) as the type strain.
authors.affiliation: Laboratoire de Bactériologie - Virologie, Hôpital de la Timone, CNRS UMR 6020, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France.
authors.forename: Olga O
authors.initials: OO
authors.lastname: Glazunova
authors.affiliation: Laboratoire de Bactériologie - Virologie, Hôpital de la Timone, CNRS UMR 6020, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.affiliation: Laboratoire de Bactériologie - Virologie, Hôpital de la Timone, CNRS UMR 6020, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Roux
mesh_terms: D016863:Bacillaceae Infections / Q000134:cerebrospinal fluid / Q000382:microbiology*; D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D002555:Cerebrospinal Fluid / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D005840:Gentian Violet; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010619:Phenazines; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005840:Gentian Violet