Cytokine and C-reactive protein profiles induced by porcine circovirus type 2 experimental infection in 3-week-old piglets.
The purpose of this study was to determine serum profiles of cytokines at a protein level and Creactive protein (CRP) during the development of postweaning multisystemic wasting syndrome (PMWS) in experimentally inoculated pigs. Levels of serum IFN-alpha, IL-6, IL-10, and CRP were examined for a 35-day period in 10 piglets experimentally infected with PCV2 at 3 weeks of age. Four of the infected piglets developed severe PMWS at 14 to 21 days post-infection (d.p.i.) and died prior to termination of the experiment. The remaining six PCV2-infected piglets experienced transient fever, but did not display overt clinical signs of PMWS and were considered as subclinically infected. A bioassay was used to detect IL-6 and ELISAs were used to detect IFN-alpha, IL-10, and CRP. There were no significant differences in cytokine or CRP expression from 0 to 7 d.p.i. between the PMWS-affected and the subclinically infected piglets. Levels of IL-10 and CRP were elevated from 10 and 14 d.p.i. respectively in the PMWS-affected piglets compared to the subclinically infected piglets. There were no significant differences in IFN-alpha and IL-6 expression between the PMWS-affected piglets and the subclinically infected piglets. The present study shows that elevated levels of serum CRP and IL-10 were associated with PCV2-infected piglets that subsequently developed severe PMWS. This may help to provide further insight into the immunoaetiogenesis of this syndrome.
Stevenson
Leanne S
LS
Department of Veterinary Science, Queen's University Belfast, Stoney Road, Stormont, Belfast, United Kingdom. s.stevenson@qub.ac.uk
McCullough
Ken
K
Vincent
Isabelle
I
Gilpin
Deirdre F
DF
Summerfield
Artur
A
Nielsen
Jens
J
McNeilly
Francis
F
Adair
Brian M
BM
Allan
Gordon M
GM
D000818:Animals; D000831:Animals, Newborn / Q000821:virology*; D002097:C-Reactive Protein / Q000378:metabolism*; D018173:Circoviridae Infections / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary / Q000821:virology; D017925:Circovirus / Q000276:immunology / Q000472:pathogenicity*; D016207:Cytokines / Q000097:blood*; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D019282:Wasting Syndrome / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D014886:Weaning
D016207:Cytokines; D002097:C-Reactive Protein