title: [Glycolytic and fatty acid metabolic enzyme changes early after acute myocardial ischemia].
abstract: OBJECTIVE
  To explore the changes of mRNA and protein expressions of glycolytic and fatty acid metabolic enzymes early after acute myocardial ischemia.
  
  
  METHODS
  Twelve dogs were randomly divided into 3 groups (sham, 20 min ischemia and 40 min ischemia, n = 4 each). Myocardial samples from ischemic and nonischemic zone were obtained for histology examination, and the mRNA expressions for Phosphofructokinase (PFK), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), GLUT1, GLUT4, Medium-chain acyl-CoA dehydrogenase (MCAD) and Heart-fatty acid binding protein (H-FABP) were determined by Real Time PCR-SYBR Green RT-PCR. GLUT1 protein expression was determined by immunohistochemistry. The apoptotic cardiomyocytes was evaluated by TUNEL.
  
  
  RESULTS
  Compared to sham hearts, H-FABP mRNA was decreased in nonischemic and ischemic zone (P < 0.05) while GLUT1 mRNA expression was significantly increased in nonischemic and ischemic zone (P < 0.05) in dogs underwent 20 and 40 min ischemia. PFK mRNA tended to be higher in ischemic myocardium (P = 0.065) and GAPDH, MCAD as well as GLUT4 remained unchanged post ischemia (all P > 0.05). Positive GLUT1 protein staining was visualized in ischemic myocardium of hearts underwent 20 and 40 min ischemia. The myocardial apoptosis cells was 6.4% +/- 0.9% in sham hearts, 28.0% +/- 3.7% in hearts underwent 20 min ischemia (P < 0.05 vs. sham) and 38.4% +/- 1.9% in hearts underwent 40 min ischemia (P < 0.05 vs. sham).
  
  
  CONCLUSIONS
  Significant down and up-regulated glycolytic and fatty acid metabolic enzymes early after myocardial ischemia suggested that these enzymes might play an important role in acute myocardial ischemia.
authors.affiliation: Department of Anaesthetization, Cardiovascular Institute, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gong
authors.forename: Li-huan
authors.initials: LH
authors.lastname: Li
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Pei
authors.forename: Hong-yue
authors.initials: HY
authors.lastname: Wang
authors.forename: Ying-li
authors.initials: YL
authors.lastname: Zheng
authors.forename: Gui-ying
authors.initials: GY
authors.lastname: Zhou
authors.forename: Shi-yong
authors.initials: SY
authors.lastname: Shi
authors.forename: Bin
authors.initials: B
authors.lastname: Guan
authors.forename: Yan-wan
authors.initials: YW
authors.lastname: Zhang
authors.forename: Zuo-xiang
authors.initials: ZX
authors.lastname: He
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005227:Fatty Acids / Q000378:metabolism*; D006019:Glycolysis; D017202:Myocardial Ischemia / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D005227:Fatty Acids; D012333:RNA, Messenger