cAMP regulates vasopressin-induced AQP2 expression via protein kinase A-independent pathway.
The regulation of AVP-induced AQP2 expression was investigated in the present study. AVP administration induced AQP2 expression in a dose-dependent manner in association with an increase in intracellular cAMP concentration. PKA activity was stimulated by AVP but PKA inhibitors did not block the upregulation of AQP2 expression. However, AVP also activated both ERK and CREB pathways, and ERK inhibitor attenuated the upregulation of AQP2 expression. These results therefore indicate that the effect of AVP stimulation to upregulate AQP2 expression involves a PKA-independent pathway.
Umenishi
Fuminori
F
Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA. Fuminori.Umenishi@UCHSC.edu
Narikiyo
Takefumi
T
Vandewalle
Alain
A
Schrier
Robert W
RW
D000818:Animals; D051399:Aquaporin 2 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D001127:Arginine Vasopressin / Q000494:pharmacology / Q000502:physiology*; D002070:Butadienes / Q000494:pharmacology; D002460:Cell Line; D016764:Cell Polarity; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism / Q000502:physiology*; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D051379:Mice; D009570:Nitriles / Q000494:pharmacology; D010766:Phosphorylation
D051399:Aquaporin 2; D002070:Butadienes; D017362:Cyclic AMP Response Element-Binding Protein; D009570:Nitriles; C113580:U 0126; D001127:Arginine Vasopressin; D005576:Colforsin; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D048049:Extracellular Signal-Regulated MAP Kinases