Advances in immunoproteomics for serological characterization of microbial antigens.
We propose a multi-dimensional strategy, associating immunodetection to a protein fractionating two-dimensional liquid chromatography tool, for serological characterization of microbial antigens. The originality of such immunoproteomic approaches resides in their application in large-scale studies for rapid serotyping of micro-organisms, evaluation of immunomes and could be proposed in the development and monitoring of vaccines.
Falisse-Poirrier
Nandini
N
Center of Research on Prion Proteins, Institute of Pharmacy-CHU, B36, Avenue de l'Hôpital 1, B-4000 University of Liège, Belgium.
Ruelle
Virginie
V
ElMoualij
Benaissa
B
Zorzi
Danièle
D
Pierard
Olivier
O
Heinen
Ernst
E
De Pauw
Edwin
E
Zorzi
Willy
W
D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology*; D001412:Bacillus subtilis / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology*; D015153:Blotting, Western; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D008853:Microscopy / Q000379:methods; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D012703:Serotyping; D013057:Spectrum Analysis; D053719:Tandem Mass Spectrometry
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D020543:Proteome