ChIP-chip comes of age for genome-wide functional analysis.
In the post-genome era, attention has focused on the functions of genome sequences and how they are regulated. The emerging epigenomic changes and the interactions between cis-acting elements and protein factors may play a central role in gene regulation. To understand the crosstalk between DNA and protein on a genome-wide scale, one emerging technique, called ChIP-chip, takes the strategy of combining chromatin immunoprecipitation with microarray. This new high-throughput strategy helps screen the targets of critical transcription factors and profile the genome-wide distribution of histone modifications, which will enable the feasibility of conducting a large-scale study, such as the Human Epigenome Project.
Wu
Jiejun
J
Departments of Molecular Genetics and Molecular Virology, Immunology, and Medical Genetics, Division of Human Cancer Genetics, Comprehensive Cancer Center, Ohio State University, Columbus, Ohio 43210, USA.
Smith
Laura T
LT
Plass
Christoph
C
Huang
Tim H-M
TH
D000818:Animals; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D023281:Genomics / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*