title: The role of OPG/TRAIL complex in multiple myeloma: the OPG/TRAIL complex in an in vitro osteoclastogenesis model derived from human multiple myeloma-bone disease.
abstract: Multiple myeloma (MM) is often associated with an increased osteoclast (OC) activity. Using an in vitro osteoclastogenesis model consisting of MM unstimulated and unfractionated peripheral blood mononuclear cells (PBMCs), we showed that T cells support OC formation and survival. Differently, in T cell-depleted MM PBMC cultures, exogenous macrophage-colony stimulating factor (M-CSF) and receptor activated of nuclear factor-kappaB ligand (RANKL) were necessary for osteoclastogenesis. We found RANKL, OPG, and TRAIL overexpression by fresh MM T cells. Despite high osteoprotegerin (OPG) levels, the persistence of osteoclastogenesis can be related to the formation of the OPG/TRAIL complex. Our results highlight that MM T cells support OC formation and survival, possibly involving OPG/TRAIL interaction.
authors.affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Piazza Giulio Cesare, 11, 70124 Bari, Italy.
authors.forename: Giacomina
authors.initials: G
authors.lastname: Brunetti
authors.forename: Silvia
authors.initials: S
authors.lastname: Colucci
authors.forename: Rita
authors.initials: R
authors.lastname: Rizzi
authors.forename: Giorgio
authors.initials: G
authors.lastname: Mori
authors.forename: Graziana
authors.initials: G
authors.lastname: Colaianni
authors.forename: Angela
authors.initials: A
authors.lastname: Oranger
authors.forename: Alberta
authors.initials: A
authors.lastname: Zallone
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Liso
authors.forename: Maria
authors.initials: M
authors.lastname: Grano
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000502:physiology*; D001847:Bone Diseases / Q000209:etiology / Q000473:pathology*; D002478:Cells, Cultured; D005260:Female; D006023:Glycoproteins / Q000502:physiology*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000502:physiology*; D008954:Models, Biological; D009101:Multiple Myeloma / Q000276:immunology / Q000473:pathology*; D010010:Osteoclasts / Q000473:pathology / Q000502:physiology*; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology*; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D051017:Apoptosis Regulatory Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; D053221:TNF-Related Apoptosis-Inducing Ligand; C506030:TNFRSF11B protein, human; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha