title: [Possible association between polymorphisms of human leukocyte antigen-DQ genes and susceptibility to trichloroethylene-induced severe generalized dermatitis].
abstract: OBJECTIVE
  To investigate the association between genetic polymorphisms of human leukocyte antigen-DQ (HLA-DQ) and susceptibility to trichloroethylene (TCE)-induced severe generalized dermatitis.
  
  
  METHODS
  A case-control study was conducted which included 112 patients with TCE-induced severe generalized dermatitis and 142 healthy controls exposed to TCE in the same workshop. The DNA sequences in exon2 of HLA-DQA1 and HLA-DQB1 were performed by direct sequencing of polymerase chain reaction (PCR) products. The frequencies distribution of allelic genotypes and codon polymorphisms were compared.
  
  
  RESULTS
  The frequencies of DQA1*0201 and 060101/0602 in cases [7.6% (17/224) and 16.1% (36/224)] were significantly higher than those of the exposed controls [3.5% (10/284) and 7.0% (20/284)], while frequencies of DQA1*0103 and 050101/0503/0505 in cases [5.8% (13/224) and 8.9% (20/224)] were significantly lower than those of exposed controls [10.9% (31/284) and 17.3% (49/284)]. In terms of codon polymorphisms, there were 5 codons of DQA1 (25, 41, 52, 54 and 69) showing significant differences between cases and controls. There were no significant differences in the frequencies of allelic genotypes of HLA-DQB1 between cases and exposed controls.
  
  
  CONCLUSIONS
  The genetic polymorphisms of HLA-DQA1 might be one of the factors influencing the individual susceptibility to TCE-induced severe generalized dermatitis.
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Hai-shan
authors.initials: HS
authors.lastname: Li
authors.forename: Yu-fei
authors.initials: YF
authors.lastname: Dai
authors.forename: Han-lin
authors.initials: HL
authors.lastname: Huang
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Feng
authors.forename: Yao-feng
authors.initials: YF
authors.lastname: Sun
authors.forename: Yong
authors.initials: Y
authors.lastname: Niu
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.forename: Lin-yuan
authors.initials: LY
authors.lastname: Zhang
authors.forename: Lai-yu
authors.initials: LY
authors.lastname: Li
authors.forename: Biao
authors.initials: B
authors.lastname: Chen
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Zheng
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D003875:Drug Eruptions / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D014241:Trichloroethylene / Q000633:toxicity*
chemical_list: D006683:HLA-DQ Antigens; D014241:Trichloroethylene