[Loss of heterozygosity at chromosome 8p21-p23 in adenocarcinoma of gastric cardia].
Chromosome 8p21-p23 harbors tumor suppressor gene(s) implicated in multiple types of cancers. To investigate the involvement of the gene(s) in the carcinogenesis of adenocarcinoma of gastric cardia, loss of heterozygosity (LOH) for microsatellite markers at chromosome 8p21-p23 was examined. Laser capture microdissection (LCM) was used to obtain homogeneous tumor cells from 19 surgical specimens. Subsequently, genomic DNA extracted from the LCM-captured cells was amplified by multiple displacement amplification. Each tumor was assessed for allelic loss using 13 microsatellite markers. An overall LOH frequency of 63.2% (12/19) was observed and the LOH frequency for individual markers varied from 25% to 55.6%. One common deleted region of about 1.2 Mb (8p22GGAA-8p22ATCT) was defined. Our data indicated that the tumor suppressor gene at chromosome 8p22 might play an important role in the development of adenocarcinoma of gastric cardia.
Man
Xiao-Hui
XH
Department of Medical Genomics, MOH Key Laboratory of Cell Biology, China Medical University, Shenyang 110001, China. manxiaohui@yahoo.com.cn
Xu
Yan
Y
Wang
Zhen-Ning
ZN
Lü
Zhi
Z
Xu
Mi-Duo
MD
Jiang
Li
L
Luo
Yang
Y
Xu
Hui-Mian
HM
Zhang
Xue
X
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D002299:Cardia / Q000473:pathology*; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D020161:Physical Chromosome Mapping; D013274:Stomach Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm