Elevated levels of gene expression for collagen and decorin in human gingival overgrowth.
OBJECTIVE
It has been demonstrated that extracellular matrix molecules are involved in cyclosporine-induced gingival overgrowth (GO). However, for many of these molecules, it remains unclear whether their abundance is modulated on the protein and gene expression level.


METHODS
To contribute to this clarification, we have analysed the protein and mRNA expression of type-I collagen (COL1) and decorin (DC) in native specimens obtained from five patients with GO, and matched normal tissue using indirect immunofluorescence (IIM), in situ hybridization (ISH) and quantitative polymerase chain reaction (PCR).


RESULTS
IIF revealed a largely co-localized although remarkably increased abundance for COL1 and DC in GO. This increase coincided with an up-regulated gene expression observed for both molecules, as detected by ISH and quantitative PCR.


CONCLUSIONS
Analysis of our data clearly demonstrates elevated levels for COL1 and DC and shows for the first time in native human tissue that involvement of these genes in GO is not confined to the protein level but also includes the transcriptional level.
Dannewitz
Bettina
B
Section of Periodontology, Department of Operative Dentistry and Periodontology, University of Heidelberg, Heidelberg, Germany.
Edrich
Christina
C
Tomakidi
Pascal
P
Kohl
Annette
A
Gabbert
Olaf
O
Staehle
Hans-Jörg
HJ
Steinberg
Thorsten
T
D000328:Adult; D016022:Case-Control Studies; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D016572:Cyclosporine / Q000009:adverse effects; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D019214:Gingival Overgrowth / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects; D017403:In Situ Hybridization; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D011509:Proteoglycans / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic; D015854:Up-Regulation; D014746:Vimentin / Q000096:biosynthesis
D024042:Collagen Type I; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D007166:Immunosuppressive Agents; D011509:Proteoglycans; D012333:RNA, Messenger; D014746:Vimentin; D016572:Cyclosporine