Genotyping of recombinant Pichia pastoris strains.
A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production.
Viader-Salvadó
José M
JM
Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Col. Ciudad Universitaria, San Nicolás de los Garza, NL, Mexico. jviader@fcb.uanl.mx
Cab-Barrera
Eddy L
EL
Galán-Wong
Luis J
LJ
Guerrero-Olazarán
Martha
M
D005838:Genotype; D030781:Organisms, Genetically Modified / Q000235:genetics*; D010802:Phylogeny; D010843:Pichia / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic