title: Sarcolemma-specific autoantibodies in canine inflammatory myopathy.
abstract: Inflammatory myopathies (IM) are relatively common in dogs with an increased incidence in the Boxer and Newfoundland breeds. Here, we show that a high proportion of affected Boxers and Newfoundlands have circulating autoantibodies against unknown sarcolemma antigens, that are muscle-specific but not species specific. We further show that the autoantigen can be extracted from muscle membranes with non-ionic detergent, and that such detergent extracts can be used in a sensitive ELISA for detection and quantitation of antibodies. The relatively high incidence of IM with autoantibodies in selected breeds of dogs indicates a genetic predisposition for a particular form of IM. In these breeds, this form of IM could be diagnosed and monitored with a simple serum assay.
authors.affiliation: The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Sabine
authors.initials: S
authors.lastname: Hankel
authors.forename: G Diane
authors.initials: GD
authors.lastname: Shelton
authors.forename: Eva
authors.initials: E
authors.lastname: Engvall
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D001707:Biopsy, Needle; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D020022:Genetic Predisposition to Disease; D008297:Male; D018482:Muscle, Skeletal / Q000276:immunology / Q000473:pathology; D009220:Myositis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D012508:Sarcolemma / Q000276:immunology* / Q000473:pathology
chemical_list: D001323:Autoantibodies