title: Comparative proteomic analysis between the invasive and commensal strains of Staphylococcus epidermidis.
abstract: Staphylococcus epidermidis is one of the major causative agents for nosocomial infections. To reveal the pathogenesis factors, we performed the comparative proteomic analysis of invasive ATCC35984 and commensal ATCC12228 strains by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. The differentially expressed proteins were involved in carbohydrate metabolism, sugar binding, lipid degradation and amino acid binding. In addition, we demonstrated that the trap gene was transcribed by 3.657+/-0.156 (P<0.01) -fold higher in ATCC35984 than in ATCC12228. Levels of accumulation-associated protein (AAP) were found to be low by the immuno-dot blotting assay in ATCC12228, which is unable to form biofilm. Our results suggest that the target of RNAIII activating protein and AAP may contribute to S. epidermidis virulence and biofilm formation.
authors.affiliation: Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Yang
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Jie-Min
authors.initials: JM
authors.lastname: Chen
authors.forename: Yuan-Zhu
authors.initials: YZ
authors.lastname: Ou
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: Hao-Jie
authors.initials: HJ
authors.lastname: Lu
authors.forename: Yu-Li
authors.initials: YL
authors.lastname: Zhu
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Qin
authors.forename: Di
authors.initials: D
authors.lastname: Qu
authors.forename: Peng-Yuan
authors.initials: PY
authors.lastname: Yang
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018441:Biofilms / Q000254:growth & development; D050260:Carbohydrate Metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D050356:Lipid Metabolism; D040901:Proteomics / Q000379:methods; D016601:RNA-Binding Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013212:Staphylococcus epidermidis / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; C079092:MtrB protein, Bacteria; D016601:RNA-Binding Proteins; D014157:Transcription Factors