Microcoil high-resolution magic angle spinning NMR spectroscopy.
We report the construction of a dual-channel microcoil nuclear magnetic resonance probehead allowing magic-angle spinning for mass-limited samples. With coils down to 235 mum inner diameter, this allows high-resolution solid-state NMR spectra to be obtained for amounts of materials of a few nanoliters. This is demonstrated by the carbon-13 spectrum of a tripeptide and a single silk rod, prepared from the silk gland of the Bombyx mori silkworm. Furthermore, the microcoil allows for radio frequency field strengths well beyond current probe technology, aiding in getting the highest possible resolution by efficiently decoupling the observed nuclei from the abundantly present proton nuclei.
Janssen
Hans
H
Department of Physical Chemistry/Solid-State NMR, Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.
Brinkmann
Andreas
A
van Eck
Ernst R H
ER
van Bentum
P Jan M
PJ
Kentgens
Arno P M
AP
D000409:Alanine / Q000737:chemistry; D000818:Animals; D012831:Bombyx / Q000737:chemistry; D004867:Equipment Design; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D012680:Sensitivity and Specificity; D047011:Silk / Q000737:chemistry
D047011:Silk; D000409:Alanine