Antitumor effects and blood flow dynamics after photodynamic therapy using benzoporphyrin derivative monoacid ring A in KLN205 and LM8 mouse tumor models.
Photodynamic therapy (PDT) using benzoporphyrin derivative monoacid ring A (BPD-MA) induces direct tumor cell damage and microvascular injury. We administered BPD-MA at 3h or 15min before laser irradiation to KLN205 and LM8 tumors in murine models. Tumor growth delay was induced more effectively by 15-min-interval PDT than by 3-h-interval PDT. Vascularity and blood perfusion was significantly decreased by 15-min-interval PDT. We observed death of all tumor cells, except peripheral cells, in the 3-h-interval PDT group, and death of cells around the damaged tumor vasculature in the 15-min-interval PDT group. Thus, 15-min-interval PDT enhanced the antitumor effect by damaging tumor vasculature.
Osaki
Tomohiro
T
Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan. ohchan@vetmed.hokudai.ac.jp
Takagi
Satoshi
S
Hoshino
Yuki
Y
Okumura
Masahiro
M
Fujinaga
Toru
T
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001783:Blood Flow Velocity / Q000187:drug effects; D015153:Blotting, Western; D045744:Cell Line, Tumor; D005260:Female; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D017078:Laser-Doppler Flowmetry; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010778:Photochemotherapy / Q000379:methods*; D011166:Porphyrins / Q000627:therapeutic use*; D013997:Time Factors; D047368:Tumor Burden / Q000187:drug effects; D000077362:Verteporfin
D000970:Antineoplastic Agents; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D011166:Porphyrins; D000077362:Verteporfin