Changes in enzymes associated with energy metabolism during the early post mortem period in longissimus thoracis bovine muscle analyzed by proteomics.
Changes in metabolic protein levels in biopsies during the early post mortem period in the bovine longissimus thoracis muscle were investigated by 2-DE based proteome analyses. Nine NRF (Norwegian Red) dual purpose bulls were included in the study. Twenty-four proteins underwent changes between the two sampling times and were classified into two major groups: metabolic proteins and heat shock proteins. Of the metabolic proteins, 5 enzymes involved in the glycolytic pathway and the tricarboxylic acid (TCA) cycle, increased in intensities during the post mortem period. In addition, the NADP-dependent enzyme 3-hydroxyisobutyrate dehydrogenase, associated with the TCA cycle in muscle, was increased. This documents that an increased aerobic energy metabolism occurs immediately after slaughter, with the aim to replenish the ATP levels in the muscle.
Jia
Xiaohong
X
Matforsk AS, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway.
Hildrum
Kjell I
KI
Westad
Frank
F
Kummen
Eiliv
E
Aass
Laila
L
Hollung
Kristin
K
D000818:Animals; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D008297:Male; D008954:Models, Biological; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D016622:Silver Staining; D013997:Time Factors
D009124:Muscle Proteins; D020543:Proteome