title: Chryseobacterium wanjuense sp. nov., isolated from greenhouse soil in Korea.
abstract: A taxonomic study was performed on strain R2A10-2(T), isolated from greenhouse soil cultivated with lettuce (Lactuca sativa L.), collected in Wanju Province, Korea. The bacterial cells were Gram-negative, aerobic, short rods. The growth temperature and pH were 5-35 degrees C and 5.0-9.0, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this isolate had 93.3-97.7 % similarity to Chryseobacterium species: the highest sequence similarities were to the type strains of Chryseobacterium daecheongense (97.7 %), Chryseobacterium formosense (97.1 %) and Chryseobacterium defluvii (96.9 %). Low levels of DNA-DNA relatedness were found between strain R2A10-2(T) and the type strains of these three species (<28 %). Differences in phenotypic properties were found with respect to Chryseobacterium species with validly published names. The predominant cellular fatty acids were iso-15 : 0 (40.0 %), iso-17 : 0 3-OH (21.9 %), iso-17 : 1omega9c (11.7 %) and summed feature 4 (iso-15 : 0 2-OH and/or 16 : 1omega7c/t, 11.0 %). Menaquinone MK-6 was detected as the sole respiratory quinone. The G+C content of the genomic DNA was 37.8 mol%. On the basis of the genomic and phenotypic evidence, this isolate represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium wanjuense sp. nov. is proposed. The type strain is R2A10-2(T) (=KACC 11468(T)=DSM 17724(T)).
authors.affiliation: Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
authors.forename: Hang-Yeon
authors.initials: HY
authors.lastname: Weon
authors.affiliation: Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Byung-Yong
authors.initials: BY
authors.lastname: Kim
authors.affiliation: Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Yoo
authors.affiliation: Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Yang-Hee
authors.initials: YH
authors.lastname: Cho
authors.affiliation: Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Seung-Joo
authors.initials: SJ
authors.lastname: Go
authors.affiliation: Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
authors.forename: Erko
authors.initials: E
authors.lastname: Stackebrandt
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D045247:Chryseobacterium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D020459:Genes, rRNA; D005840:Gentian Violet; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D010619:Phenazines; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D005840:Gentian Violet