Expression of bovine parathyroid hormone/parathyroid hormone- related protein (PTH/PTHrP) receptor mRNA in the mammary gland of cows.
To examine the PTH/PTHrP receptor in the mammary gland, molecular cloning of bovine PTH/PTHrP receptor and measurement of its mRNA expression were carried out in cows during the periparturient period. The PTH/PTHrP receptor gene was partially cloned, and expression of bovine PTH/PTHrP receptor mRNA was detected in various tissues of the cow. In the mammary gland, PTH/PTHrP receptor mRNA expression was constantly low during the periparturient period, whereas PTHrP mRNA expression dramatically increased after parturition. This suggested that expression of PTH/PTHrP receptor mRNA in the mammary gland is not affected by lactation in cows.
Onda
Ken
K
Laboratory of Internal Medicine III, Azabu University School of Veterinary Medicine, Kanagawa, Japan.
Matsuki
Naoaki
N
Ono
Kenichiro
K
Wada
Yasunori
Y
D000818:Animals; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D007774:Lactation / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism*; D036801:Parturition / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044168:Receptor, Parathyroid Hormone, Type 1 / Q000235:genetics*
D012333:RNA, Messenger; D044168:Receptor, Parathyroid Hormone, Type 1