Preparation, characterization and cytocompatibility of porous ACP/PLLA composites.
The purpose of this work was to incorporate amorphous calcium phosphate (ACP) into porous poly(L-lactic acid) (PLLA), because ACP is capable of fast phase transformation and morphological change in body fluid, such, a desired pore wall surface within bone tissue engineering scaffolds can be created. A highly porous ACP/PLLA composite was prepared by a thermally induced phase separation technique. The results showed that the composite had an interconnected pore structure with 100 mum macropores and 10 mum micropores, and 91% porosity; 40 nm primary particles of ACP were agglomerated to 3 mum aggregates, and the aggregates were homogeneously distributed in pore walls; These aggregates showed to be in situ transformed into bone-like apatite after 1 h soaking in phosphate buffered saline solution. Human osteoblast-like cell culture showed that the ACP/PLLA composite had better cell adhesion and alkaline phosphotase activity than pure PLLA. This study demonstrates that the ACP/PLLA composite can enhance cytocompatibility and could act as a promising scaffold for bone tissue engineering.
Gao
Yanbo
Y
Department of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Weng
Wenjian
W
Cheng
Kui
K
Du
Piyi
P
Shen
Ge
G
Han
Gaorong
G
Guan
Binggang
B
Yan
Weiqi
W
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D002460:Cell Line; D006801:Humans; D019344:Lactic Acid; D011091:Polyesters; D011108:Polymers
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; C519480:amorphous calcium phosphate; D019344:Lactic Acid; C033616:poly(lactide); C020243:calcium phosphate