[Synchronous fluorimetric determination of the residues of three quinolones in Eel by partial least squares].
In the present paper, the synchronous fluorimetric spectra of norfloxacin, lomefloxacin chlorhydric acid, and levofloxacin lactate with deltalambda = 190 nm in a B-R buffer medium of pH 2.87 were measured, and the partial least squares (PLS) method was applied to the quantitative resolution of the seriously overlapped fluorimetric spectra of these compounds. The linear ranges for norfloxacin, lomefloxacin chlorhydric acid, and levofloxacin lactate are 0.016-0.40 microg x mL(-1), 0.01-0.336 microg x mL(-1) and 0.01-0.336 microg x mL(-1), respectively. The limits of detection are 0.012 6 microg x mL(-1) for norfloxacin, 0.006 microg x mL(-1) for lomefloxacin chlorhydric acid, and 0.007 2 microg x mL(-1) for levofloxacin lactate. The analytical results by PLS were compared with principal components regression (PCR) and classical least squares (CLS), and this method was applied to the determination of these three compounds in eel samples with satisfactory results.
Zhang
Guo-wen
GW
The Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China.
Ni
Yong-nian
YN
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D004524:Eels; D005470:Fluorometry / Q000379:methods; D024841:Fluoroquinolones / Q000032:analysis; D016018:Least-Squares Analysis; D064704:Levofloxacin; D009643:Norfloxacin / Q000032:analysis; D015242:Ofloxacin / Q000032:analysis; D015363:Quinolones / Q000032:analysis*; D017747:Seafood / Q000032:analysis*
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D015363:Quinolones; D064704:Levofloxacin; D015242:Ofloxacin; C053091:lomefloxacin; D009643:Norfloxacin