[Study on the interaction mechanism of targeting molecules carboxymethyl polysaccharide with DNA].
The interaction of chitosan (CTS), carboxymethyl chitosans (CMCTS), and carboxymethyl cellulose (CMCL) with DNA was investigated through ethidium bromide (EB) as a sensitive fluorescence probe, and ultraviolet absorption spectra. The constant D can directly denote the degree of interaction of polysaccharide with DNA. The results indicated that the interraction sequence of biological polysaccharides with DNA is as follows: CTS>CMCTS>CMCL. The polysaccharides were mainly bound to DNA by intercalated binding, which resulted in fluorescence quenching effect of EB-DNA.
Zhang
Hai-rong
HR
Laboratory of Biochemical Analysis, Xinzhou Teacher's University, China.
Guo
Si-yuan
SY
Li
Lin
L
Cai
Miao-yan
MY
D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000031:analogs & derivatives* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans
D005456:Fluorescent Dyes; C514968:carboxymethyl-chitosan; C052890:ethidium-DNA complex; D004247:DNA; D048271:Chitosan; D004996:Ethidium