title: Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines.
abstract: BACKGROUND
  Malignant melanoma resistance to chemotherapy remains a major limitation to treatment. Our aim was to identify genes associated with drug resistance, in order to better understand the molecular events underlying the drug-resistant phenotype.
  
  
  METHODS
  A human melanoma cell line and its drug-resistant variants obtained by selection with MNNG or 6-thioguanine were used. Alterations in gene expression were characterized by differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Prominent mRNA fragments present in selected variants and not in the parental cells were identified and characterized by cloning and sequencing. Differential expression was confirmed by real-time RT-PCR.
  
  
  RESULTS
  Three functionally distinct transcriptional products were demonstrated: the chaperonin subunit TCP 1-zeta-6A (CCT6A), the hyaluronan receptor CD44 and LPPR-2, the lipid phosphate phosphatase-related protein type-2.
  
  
  CONCLUSIONS
  Genes with altered expression were identified in drug-resistant variants. The identified molecules may provide new insights into the molecular basis for melanoma resistance to chemotherapy.
authors.affiliation: Department of Neurobiology and Immunology, Institute of Biological Research "Sinica Stankovic", Belgrade, Israel.
authors.forename: Nikola
authors.initials: N
authors.lastname: Tanic
authors.forename: Gordana
authors.initials: G
authors.lastname: Brkic
authors.forename: Bogomir
authors.initials: B
authors.lastname: Dimitrijevic
authors.forename: Nasta
authors.initials: N
authors.lastname: Dedovic-Tanic
authors.forename: Nir
authors.initials: N
authors.lastname: Gefen
authors.forename: Daniel
authors.initials: D
authors.lastname: Benharroch
authors.forename: Jacob
authors.initials: J
authors.lastname: Gopas
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D019008:Drug Resistance, Neoplasm; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008545:Melanoma / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D015203:Reproducibility of Results
chemical_list: D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012333:RNA, Messenger