title: Ionizing radiation suppresses FAP-1 mRNA level in A549 cells via p53 activation.
abstract: Ionizing radiation (IR) is known to upregulate cell surface Fas through p53 activation in various cells. However, the signaling pathway intermediating between p53 activation and cell surface Fas upregulation remains to be elucidated. Recently, Fas-associated phosphatase-1 (FAP-1) has been reported to associate with Fas and inhibit cell surface Fas expression. We evaluated the expression of FAP-1 mRNA following IR in A549 cells. Ionizing radiation inhibited the expression of FAP-1 mRNA. Pretreatment with p53 inhibitor pifithrin alpha cancelled the IR-induced downregulation of FAP-1 mRNA. These results suggest that IR-induced p53 activation may upregulate cell surface Fas via the down-modulation of FAP-1.
authors.affiliation: The First Department of Internal Medicine, Faculty of Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Yamada
authors.forename: Muneharu
authors.initials: M
authors.lastname: Maruyama
authors.forename: Tadashi
authors.initials: T
authors.lastname: Fujita
authors.forename: Koutarou
authors.initials: K
authors.lastname: Miyabayashi
authors.forename: Chie
authors.initials: C
authors.lastname: Shinoda
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kawagishi
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujishita
authors.forename: Ryuji
authors.initials: R
authors.lastname: Hayashi
authors.forename: Toshiro
authors.initials: T
authors.lastname: Miwa
authors.forename: Nobuki
authors.initials: N
authors.lastname: Arai
authors.forename: Shoko
authors.initials: S
authors.lastname: Matsui
authors.forename: Eiji
authors.initials: E
authors.lastname: Sugiyama
authors.forename: Masashi
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D052160:Benzothiazoles; D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D054645:Protein Phosphatase 1; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011839:Radiation, Ionizing; D013844:Thiazoles / Q000494:pharmacology; D014050:Toluene / Q000031:analogs & derivatives / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D052160:Benzothiazoles; D012333:RNA, Messenger; D013844:Thiazoles; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor; D014050:Toluene; C121565:pifithrin; D054645:Protein Phosphatase 1; C516742:PTPN13 protein, human; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13; D017027:Protein Tyrosine Phosphatases