Immunological images of polycyclic aromatic hydrocarbons.
OBJECTIVE
To develop experimental model for definition of immunological images of chemical carcinogens.


METHODS
The conjugates of benzo[a]pyrene, benz[a]anthracene, anthracene, chrysene and pyrene with bovine serum albumin and yeast hexokinase were synthesized. Rabbits were immunized by bovine serum albumin-hapten conjugates. Antibodies to each hapten were isolated from the serum by affinity chromatography with the hapten-yeast hexokinase-Sepharose sorbents. The binding of each hapten with each antibody was determined by competitive immunoassay.


RESULTS
The immunological images of all the investigated chemical compounds were described.


CONCLUSIONS
The model is proposed to determine the internal immunological images of anti-idiotypic monoclonal antibodies to chemical carcinogens.
Glushkov
A N
AN
Institute of Human Ecology, Siberian Branch of Russian Academy of Sciences, Kemerovo, Russia. ihe@kemtel.ru
Kostyanko
M V
MV
Anosova
T P
TP
Polenok
E G
EG
Mun
S A
SA
Anosov
M P
MP
Cherno
S V
SV
D000818:Animals; D000906:Antibodies / Q000097:blood / Q000302:isolation & purification; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002273:Carcinogens / Q000737:chemistry; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D006593:Hexokinase / Q000737:chemistry / Q000276:immunology; D007114:Immunization; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002273:Carcinogens; D006241:Haptens; D011084:Polycyclic Aromatic Hydrocarbons; D012710:Serum Albumin, Bovine; D006593:Hexokinase