title: Affinity liquid chromatography and capillary electrophoresis of seminal plasma proteins.
abstract: Interactions of boar, bull, and human seminal plasma proteins with heparin and phosphorylcholine were studied by affinity LC using heparin immobilized to a Toyopearl support. A step gradient elution from 0.15 to 1.50 M NaCl was employed to elute the seminal plasma proteins. Relative amounts of the heparin-binding fraction of seminal plasma proteins (H+) in seminal plasma of three species were determined. Further on, the fraction of seminal plasma proteins interacting with phosphorylcholine-binding proteins (P+) was evaluated. P+ proteins were not found in human seminal plasma and their highest amount was present in bull seminal plasma. A CE method was developed for separation of seminal plasma proteins. Various capillaries and separation conditions were tested; the best resolution was obtained in a bare-silica capillary, with a micellar system consisting of a 0.02 M borate buffer and 0.05 M SDS pH 10.0. The optimized conditions were applied to the identification of the components in boar plasma.
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic. tereza.varilova@seznam.cz
authors.forename: Tereza
authors.initials: T
authors.lastname: Varilová
authors.forename: Hana
authors.initials: H
authors.lastname: Seménková
authors.forename: Pavel
authors.initials: P
authors.lastname: Horák
authors.forename: Milan
authors.initials: M
authors.lastname: Madera
authors.forename: Vera
authors.initials: V
authors.lastname: Pacáková
authors.forename: Marie
authors.initials: M
authors.lastname: Tichá
authors.forename: Karel
authors.initials: K
authors.lastname: Stulík
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006493:Heparin / Q000737:chemistry; D006801:Humans; D008297:Male; D010767:Phosphorylcholine / Q000737:chemistry; D020033:Protein Isoforms / Q000032:analysis; D029589:Seminal Plasma Proteins / Q000032:analysis*
chemical_list: D020033:Protein Isoforms; D029589:Seminal Plasma Proteins; D010767:Phosphorylcholine; D006493:Heparin