title: [Application of fluorescence in-situ hybridization and reverse transcription-polymerase chain reaction in molecular diagnosis of Ewing's sarcoma and primitive neuroectodermal tumor].
abstract: OBJECTIVE
  To detect tumor specific chromosome translocations and associated fusion transcripts in paraffin-embedded tissue by interphase fluorescence in-situ hybridization (FISH) and reverse transcription polymerase chain reaction (RT-PCR), respectively, and to evaluate their diagnostic values for Ewing's sarcoma/primitive neuroectodermal tumor (ES/PNET).
  
  
  METHODS
  Nuclei of the tumor cells and total RNA were extracted from 10 cases of ES/PNET. Interphase FISH was utilized to analyze the EWS gene translocation with a dual color, break apart probe (Vysis company). RT-PCR was used to detect t (11; 22) (q24; q12) and t (21; 22) (q22; q12) fusion transcripts.
  
  
  RESULTS
  Among 10 cases of ES/PNET, the EWS-FLI1 fusion transcript was detected in 8 by RT-PCR. EWS-ERG fusion transcript was not detected in any of the cases. EWS gene translocation was found in 9 of 10 cases by FISH.
  
  
  CONCLUSIONS
  Interphase FISH and RT-PCR can be reliably applied to paraffin-embedded tissues for molecular diagnosis of ES/PNET. Between the two approaches, interphase FISH provides a more sensitive and stable result.
authors.affiliation: Department of Pathology, Health Science Center, Peking University, Beijing 100083, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Yan-yu
authors.initials: YY
authors.lastname: Wei
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiao-long
authors.initials: XL
authors.lastname: Ma
authors.forename: Xiu-ping
authors.initials: XP
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012512:Sarcoma, Ewing / Q000175:diagnosis* / Q000235:genetics