title: Lack of telomerase activity in rabbit bone marrow stromal cells during differentiation along neural pathway.
abstract: OBJECTIVE
  To investigate telomerase activity in rabbit bone marrow stromal cells (BMSCs) during their committed differentiation in vitro along neural pathway and the effect of glial cell line-derived neurotrophic factor (GDNF) on the expression of telomerase.
  
  
  METHODS
  BMSCs were acquired from rabbit marrow and divided into control group, GDNF (10 ng/ml) group. Cytokine.NSCs medium (prepared by our lab, Patent No. ZL02134314. 4) supplemented with 10 percent fetal bovine serum (FBS) was used to induce BMSCs differentiation along neural pathway. Fluorescent immunocytochemistry was employed to identify the expressions of Nestin, neuron-specific endase (NSE), and gial fibrillary acidic protein (GFAP). The growth curves of the cells and the status of cell cycles were analyzed, respectively. During the differentiation, telomerase activities were detected using the telomeric repeat amplification protocol-enzyme-linked immunosorbent assay (TRAP-ELISA).
  
  
  RESULTS
  BMSCs were successfully induced to differentiate along neural pathway and expressed specific markers of fetal neural epithelium, mature neuron and glial cells. Telomerase activities were undetectable in BMSCs during differentiation along neural pathway. Similar changes of cell growth curves, cell cycle status and telomerase expression were observed in the two groups.
  
  
  CONCLUSIONS
  Rabbit BMSCs do not display telomerase activity during differentiation along neural pathway. GDNF shows little impact on proliferation and telomerase activity of BMSCs.
authors.affiliation: Institute of Neuromedicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
authors.forename: Zhen-zhou
authors.initials: ZZ
authors.lastname: Chen
authors.forename: Ru-xiang
authors.initials: RX
authors.lastname: Xu
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Jiang
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Teng
authors.forename: Gui-tao
authors.initials: GT
authors.lastname: Li
authors.forename: Yü-xi
authors.initials: YX
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000201:enzymology*; D002454:Cell Differentiation; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007150:Immunohistochemistry; D011817:Rabbits; D017154:Stromal Cells / Q000201:enzymology; D019098:Telomerase / Q000378:metabolism*
chemical_list: D051100:Glial Cell Line-Derived Neurotrophic Factor; D019098:Telomerase