title: Immunohistochemistry as a fundamental tool for the differential diagnosis of polymorphous low-grade adenocarcinoma.
abstract: Polymorphous low-grade adenocarcinoma (PLGA) is a subtype of salivary gland carcinoma with a marked predilection for the minor salivary glands. The diagnosis of this lesion can be a challenge in incisional biopsies because of its similarity to other salivary neoplasms and the difficulty of analyzing its infiltrative nature and neurotropism. Diagnosis can be facilitated, however, by immunohistochemistry, a technique that has become a fundamental tool to the pathologist. The purpose of this study was analyze the immunohistochemical aspects of 4 cases that were examined in the São José dos Campos School of Dentistry. The patients included 4 women, ages 32 to 57 years (mean, 50 years), who reported an intraoral painless lesion, ranging in size from 1 to 3 cm (mean, 1.75 cm) with a variable evolution period. Histologically, the lesions showed similar aspects, characterized by polymorphous growth pattern, including solid, cribriform, trabecular, papillary, and "single-file" arrays. The tumors showed positivity to cytokeratins (Cks) 7 and 8 and vimentin, variable expression for Ck 14, and a negative reaction to Cks 13 and 19 and muscle-specific actin. PLGA is a tumor of the salivary gland that must be distinguished from other neoplasms for therapeutic and prognostic considerations, and the panel of antibodies employed in this study was effective for its diagnosis.
authors.affiliation: Department of Bioscience and Oral Diagnosis, São José dos Campos School of Dentistry, State University of São Paulo (UNESP), Brazil. luanamrv@itelefonica.com.br
authors.forename: Luana
authors.initials: L
authors.lastname: Marotta Reis de Vasconcellos
authors.forename: Vanessa
authors.initials: V
authors.lastname: Avila Sarmento Silveira
authors.forename: Luiz
authors.initials: L
authors.lastname: Eduardo Blumer Rosa
authors.forename: Ana
authors.initials: A
authors.lastname: Sueli Rodrigues Cavalvante
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Rodarte Carvalho
mesh_terms: D000199:Actins / Q000032:analysis; D000230:Adenocarcinoma / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D000328:Adult; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis; D008875:Middle Aged; D012468:Salivary Gland Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D012470:Salivary Glands, Minor / Q000473:pathology*; D014746:Vimentin / Q000032:analysis
chemical_list: D000199:Actins; D014746:Vimentin; D007633:Keratins