Microbial transformation of nandrolone decanoate by acremonium strictum.
Estr-4-en-3,17-dione II, 17beta-hydroxyestr-4-en-3-one III, 15alpha-hydroxyestr-4-en-3,17-dione IV, and 15alpha,17beta-dihydroxyestr-4-en-3-one V were produced by microbial transformation of nandrolone decanoate I in the culture of Acremonium strictum PTCC 5282. Bioconversion characteristics observed were ester hydrolysis, oxidation, and hydroxylation. Each microbial product was purified chromatographically and characterized on the basis of spectral data obtained from (1)H-NMR,( 13)C-NMR, FT-IR, MS, and physical constants such as melting point and optical rotation.
Yazdi
Mojtaba Tabatabaei
MT
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. mtabataba@sina.tums.ac.ir
Zanjanian
Seyedeh Maryam
SM
Faramarzi
Mohammad Ali
MA
Amini
Mohsen
M
Amani
Amir
A
Abdi
Khosrou
K
D000164:Acremonium / Q000254:growth & development / Q000378:metabolism*; D000728:Androgens / Q000737:chemistry / Q000378:metabolism; D001711:Biotransformation; D002855:Chromatography, Thin Layer / Q000379:methods; D006868:Hydrolysis; D015394:Molecular Structure; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000077603:Nandrolone Decanoate; D010084:Oxidation-Reduction
D000728:Androgens; D009277:Nandrolone; D000077603:Nandrolone Decanoate