title: Comparison among tomato juice agar with other three media for differentiation of Candida dubliniensis from Candida albicans.
abstract: The purpose of the present study is to compare the tomato juice agar, a well known medium employed to observe ascospore formation, with niger seed agar, casein agar and sunflower seed agar, applied to a differentiation between C. dubliniensis and C. albicans. After 48 hours of incubation at 30 degrees C all 26 (100%) C. dubliniensis isolates tested produced chlamydospores on tomato juice agar as well as in the other three media evaluated. However, when we inoculated all media with C. albicans, the absence of chlamydospores became resulting in the following percents: tomato juice agar (92.47%), niger seed agar (96.7%), casein agar (91.39%), and sunflower seed agar (96.7%). These results indicate that tomato juice agar is another medium which can also be used in the first phenotypic differentiation between C. dubliniensis and C. albicans.
authors.affiliation: Universidade Federal de Santa Maria, Rua Andradas 1985/201, 97010-033 Santa Maria, RS, Brasil. hartzsa@ccs.ufsm.br
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
authors.forename: Erico Silva
authors.initials: ES
authors.lastname: de Loreto
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Linares
authors.forename: Carolina P
authors.initials: CP
authors.lastname: Silveira
authors.forename: Liliane A
authors.initials: LA
authors.lastname: Scheid
authors.forename: Daniela I Brayer
authors.initials: DI
authors.lastname: Pereira
authors.forename: Janio Morais
authors.initials: JM
authors.lastname: Santuario
mesh_terms: D000362:Agar / Q000737:chemistry*; D002175:Candida / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D002176:Candida albicans / Q000145:classification / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry*; D018551:Solanum lycopersicum; D016533:Mycological Typing Techniques / Q000379:methods; D013172:Spores, Fungal / Q000254:growth & development
chemical_list: D003470:Culture Media; D000362:Agar