[Determination of tetracyclines by a new spectrum technique].
Tetracyclines are light-fluorescence substances, which cannot be detected directly by fluorometry. Herein a new spectrum method was proposed to detect tetracyclines directly by fluorometry. Under optimal conditions, the calibration graph is linear over the range 0.10-9.00 microg x mL(-1) for tetracyclines, and the detection limits of tetracycline, oxytetracycline, chlortetracycline and doxycycline are 0.065, 0.067, 0.068 and 0.070 microg x mL(-2), respectively.
Gao
Hong
H
Department of Chemistry and the Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Zhao
Yi-bing
YB
Guo
Xiang-qun
XQ
D000900:Anti-Bacterial Agents / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013754:Tetracyclines / Q000032:analysis*
D000900:Anti-Bacterial Agents; D013754:Tetracyclines