title: Scale up of proteoliposome derived Cochleate production.
abstract: Cochleate are highly stable structures with promising immunological features. Cochleate structures are usually obtaining from commercial lipids. Proteoliposome derived Cochleate are derived from an outer membrane vesicles of Neisseria meningitidis B. Previously, we obtained Cochleates using dialysis procedures. In order to increase the production process, we used a crossflow system (CFS) that allows easy scale up to obtain large batches in an aseptic environment. The raw material and solutions used in the production process are already approved for human application. This work demonstrates that CFS is very efficient process to obtain Cochleate structures with a yield of more than 80% and the immunogenicity comparable to that obtained by dialysis membrane.
authors.affiliation: Immunology Department, Finlay Institute, P.O. Box 16017, Havana, Cuba. czayas@finlay.edu.cu
authors.forename: Caridad
authors.initials: C
authors.lastname: Zayas
authors.forename: Gustavo
authors.initials: G
authors.lastname: Bracho
authors.forename: Miriam
authors.initials: M
authors.lastname: Lastre
authors.forename: Domingo
authors.initials: D
authors.lastname: González
authors.forename: Danay
authors.initials: D
authors.lastname: Gil
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Acevedo
authors.forename: Judith
authors.initials: J
authors.lastname: del Campo
authors.forename: Carlos
authors.initials: C
authors.lastname: Taboada
authors.forename: Rosa L
authors.initials: RL
authors.lastname: Solís
authors.forename: Ramón
authors.initials: R
authors.lastname: Barberá
authors.forename: Oliver
authors.initials: O
authors.lastname: Pérez
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D005260:Female; D051379:Mice; D038541:Neisseria meningitidis, Serogroup B / Q000737:chemistry; D011510:Proteolipids / Q000302:isolation & purification* / Q000494:pharmacology; D014462:Ultrafiltration
chemical_list: D000276:Adjuvants, Immunologic; D011510:Proteolipids; C020485:proteoliposomes