The adjuvant potential of synthetic alkylglycerols.
Alkylglycerols (AGs) have shown immune stimulant and adjuvant activity in many studies, but natural sources are not so accessible and their extraction from them is very complicated. Therefore, a group of chemists at IFAL have synthesized AG analogs. The aim of this work was to evaluate the adjuvant potential of different synthetic AGs. A mix of ovoalbumin (Ova) and AGs increase anti-Ova IgG antibodies production in sera of immunized mice. The predominant subclass was IgG1 although higher levels of IgG2a were observed as the carbon chain length of AGs increased. AGs also induced the production of IL-12 and nitric oxide (NO) in the U937 human histiocyte and J774 mouse macrophage cell lines, respectively. These results indicate that synthetic AGs are effective adjuvants for the standardized antigen, Ova.
Acevedo
Reinaldo
R
Department of Immunology, Finlay Institute, P.O. Box 16017, Havana, Cuba. racevedo@finlay.edu.cu
Gil
Danay
D
del Campo
Judith
J
Bracho
Gustavo
G
Valdés
Yolanda
Y
Pérez
Oliver
O
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D005990:Glycerol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006644:Histiocytes / Q000276:immunology; D006801:Humans; D008264:Macrophages / Q000276:immunology; D051379:Mice; D010047:Ovalbumin / Q000276:immunology
D000276:Adjuvants, Immunologic; D010047:Ovalbumin; D005990:Glycerol