Comparison among tomato juice agar with other three media for differentiation of Candida dubliniensis from Candida albicans.
The purpose of the present study is to compare the tomato juice agar, a well known medium employed to observe ascospore formation, with niger seed agar, casein agar and sunflower seed agar, applied to a differentiation between C. dubliniensis and C. albicans. After 48 hours of incubation at 30 degrees C all 26 (100%) C. dubliniensis isolates tested produced chlamydospores on tomato juice agar as well as in the other three media evaluated. However, when we inoculated all media with C. albicans, the absence of chlamydospores became resulting in the following percents: tomato juice agar (92.47%), niger seed agar (96.7%), casein agar (91.39%), and sunflower seed agar (96.7%). These results indicate that tomato juice agar is another medium which can also be used in the first phenotypic differentiation between C. dubliniensis and C. albicans.
Alves
Sydney Hartz
SH
Universidade Federal de Santa Maria, Rua Andradas 1985/201, 97010-033 Santa Maria, RS, Brasil. hartzsa@ccs.ufsm.br
de Loreto
Erico Silva
ES
Linares
Carlos Eduardo
CE
Silveira
Carolina P
CP
Scheid
Liliane A
LA
Pereira
Daniela I Brayer
DI
Santuario
Janio Morais
JM
D000362:Agar / Q000737:chemistry*; D002175:Candida / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D002176:Candida albicans / Q000145:classification / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry*; D018551:Solanum lycopersicum; D016533:Mycological Typing Techniques / Q000379:methods; D013172:Spores, Fungal / Q000254:growth & development
D003470:Culture Media; D000362:Agar