title: Comparative analysis by frontal affinity chromatography of oligosaccharide specificity of GlcNAc-binding lectins, Griffonia simplicifolia lectin-II (GSL-II) and Boletopsis leucomelas lectin (BLL).
abstract: Lectin-based structural glycomics requires a search for useful lectins and their biochemical characterization to profile complex features of glycans. In this paper, two GlcNAc-binding lectins are reported with their detailed oligosaccharide specificity. One is a classic plant lectin, Griffonia simplicifolia lectin-II (GSL-II), and the other is a novel fungal lectin, Boletopsis leucomelas lectin (BLL). Their sugar-binding specificity was analyzed by frontal affinity chromatography using 146 glycans (125 pyridylaminated and 21 p-nitrophenyl saccharides). As a result, it was found that both GSL-II and BLL showed significant affinity toward complex-type N-glycans, which are either partially or completely agalactosylated. However, their branch-specific features differed significantly: GSL-II strongly bound to agalacto-type, tri- or tetra-antennary N-glycans with its primary recognition of a GlcNAc residue transferred by GlcNAc-transferase IV, while BLL preferred N-glycans with fewer branches. In fact, the presence of a GlcNAc residue transferred by GlcNAc-transferase V abolishes the binding of BLL. Thus, GSL-II and BLL forms a pair of complementally probes to profile a series of agalacto-type N-glycans.
authors.affiliation: Glycostructure Analysis Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 2, 1-1-1, Umezono, Tsukuba, Ibaraki 305-8568.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nakamura-Tsuruta
authors.forename: Junko
authors.initials: J
authors.lastname: Kominami
authors.forename: Masugu
authors.initials: M
authors.lastname: Kamei
authors.forename: Yu
authors.initials: Y
authors.lastname: Koyama
authors.forename: Takuji
authors.initials: T
authors.lastname: Suzuki
authors.forename: Mamoru
authors.initials: M
authors.lastname: Isemura
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
mesh_terms: D002236:Carbohydrate Conformation; D002846:Chromatography, Affinity / Q000379:methods*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D037121:Plant Lectins