[Diagnostic value of autoantibodies to MAG by ELISA Bühlmann in 117 immune-mediated peripheral neuropathies associated with monoclonal IgM to SGPG/SGLPG].
The neuropathies associated with monoclonal IgM gammopathy reacted with glycoconjugated targets on a very antigenic epitope on the sulfated glucuronic glycolipids corresponding to SGPG and SGLPG (sulfoglucuronyl paragloboside and sulfoglucuronyl lactosaminyl paragloboside), myelin-associated glycoprotein (MAG) and sulfatide. Sometimes monoclonal IgM binds to a broad spectrum of gangliosides. The detection of targets of autoantibodies has considerable importance in the diagnosis and management of patients. It is not known whether the results of antibody tests are equally sensitive and specific for identification of involved auto-antigens. In this study we evaluated the results obtained using IgM reactivity against MAG by enzyme-linked immunosorbent assay (ELISA Bühlmann) with IgM reactivity against SGPG/SGLPG obtained by overlay thin-layer chromatography. We selected 117 patients with anti-SGPG/SGLPG monoclonal gammopathy and peripheral neuropathy and a control group of 102 peripheral neuropathies with 24 having IgM high titres of monoclonal IgM anti-ganglioside antibodies. The anti-MAG sensitivity was 0.97, specificity was 0.86. There is a crossreactivity between 8 (57%) monoclonal IgM antibodies anti-MAG and anti-ganglioside GM1 and 2 (28%) anti-disialylated gangliosides. These results indicate that in clinical practice, anti-MAG ELISA is useful for eliminating anti-MAG neuropathy, as well as for positive diagnosis for titres upper than 10,000 BTU. It is also alpha good test to appreciate clinical improvement after Rituximab treatment.
Caudie
C
C
Fédération de Biologie, Service d'Immunologie et Neuro-Immunologie, Hôpital Neurologique, Lyon Bron. christiane.caudie@chu-lyon.fr
Kaygisiz
F
F
Jaquet
P
P
Petiot
P
P
Gonnaud
P-M
PM
Antoine
J-C
JC
Vial
C
C
D000911:Antibodies, Monoclonal / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005915:Globosides / Q000097:blood / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D010265:Paraproteinemias / Q000175:diagnosis*; D010523:Peripheral Nervous System Diseases / Q000175:diagnosis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001324:Autoantigens; D005915:Globosides; D007075:Immunoglobulin M; C051917:sulfate-3-glucuronyl paragloboside; C064938:3-sulfoglucuronyl-lactosaminyl-paragloboside