title: Adjuvant properties of lipopolysaccharide from Neisseria meningitidis serogroup B detoxified and conjugated with tetanus toxoid.
abstract: We evaluated the adjuvant properties and toxicity of purified Neisseria meningitidis serogroup B lipopolysaccharide (LPS) conjugated with tetanus toxoid (TT) using a new method of conjugation to obtain amine groups in the polysaccharide structure. The endotoxic activity of treated LPS was reduced 2400 times as determined by Limulus amoebocyte assay and no mortality was observed in Balb/c mice inoculated with detoxified LPS versus 100% mortality in native LPS inoculated mice. The conjugated LPS-TT elicited in mice higher anti-TT IgG2a and IgG1 than unconjugated TT. In addition, high levels of anti-LPS IgG and IgG subclasses were detected in sera. These results evidence the adjuvant activity of detoxified LPS and may suggest that the conjugation to TT changes the LPS immune response from thymus-independent to thymus-dependent.
authors.affiliation: Immunology Department, Finlay Institute, P.O. Box 16017, Havana, Cuba jbalboa@finlay.edu.cu
authors.forename: Julio A
authors.initials: JA
authors.lastname: Balboa
authors.forename: Maribel
authors.initials: M
authors.lastname: Cuello
authors.forename: Osmir
authors.initials: O
authors.lastname: Cabrera
authors.forename: Judith
authors.initials: J
authors.lastname: del Campo
authors.forename: Miriam
authors.initials: M
authors.lastname: Lastre
authors.forename: Danay
authors.initials: D
authors.lastname: Gil
authors.forename: Carlos
authors.initials: C
authors.lastname: Taboada
authors.forename: Mildrey
authors.initials: M
authors.lastname: Fariñas
authors.forename: Marixa
authors.initials: M
authors.lastname: Hernández
authors.forename: Oliver
authors.initials: O
authors.lastname: Pérez
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D038541:Neisseria meningitidis, Serogroup B; D013745:Tetanus Toxoid / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D013745:Tetanus Toxoid; D018074:Vaccines, Conjugate