title: A new capillary zone electrophoresis method for the analysis of Ginkgo biloba extracts and Ginkgo biloba pharmaceutical products.
abstract: Ginkgo biloba, traditional Chinese medicine is now generally accepted. Separation and determination of active components in G. biloba is important for the product quality control. Therefore, the development of an effective and reliable separation method is important. In this work, a new capillary electrophoretic (CZE) method for separation of the G. biloba leaf extracts components was developed and optimized by the use of experimental design and artificial neural network (ANN). Under best separation conditions, in gamma-CD-modified buffer, the separation was reached within 10 min (36 mM borate BGE, pH 9.2, 1 mM gamma-CD), while the hydrodynamic mode for sample injection (2 s) and UV detection at 270 nm were applied. The method developed was validated and applied for analysis of various extracts and G. biloba products.
authors.affiliation: Department of Analytical Chemistry, Masaryk University, Kotlárská, Brno, Czech Republic. Shabanelosta@yahoo.co.uk
authors.forename: Shaban
authors.initials: S
authors.lastname: Elosta
authors.forename: Dagmar
authors.initials: D
authors.lastname: Gajdosová
authors.forename: Josef
authors.initials: J
authors.lastname: Havel
mesh_terms: D003505:Cyclodextrins / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D008516:Medicine, Chinese Traditional; D016571:Neural Networks, Computer; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D003505:Cyclodextrins; D004365:Drugs, Chinese Herbal; D004364:Pharmaceutical Preparations; D010936:Plant Extracts