title: Looking towards label-free biomolecular interaction analysis in a high-throughput format: a review of new surface plasmon resonance technologies.
abstract: Surface plasmon resonance (SPR) biosensors have enabled a wide range of applications in which researchers can monitor biomolecular interactions in real time. Owing to the fact that SPR can provide affinity and kinetic data, unique features in applications ranging from protein-peptide interaction analysis to cellular ligation experiments have been demonstrated. Although SPR has historically been limited by its throughput, new methods are emerging that allow for the simultaneous analysis of many thousands of interactions. When coupled with new protein array technologies, high-throughput SPR methods give users new and improved methods to analyze pathways, screen drug candidates and monitor protein-protein interactions.
authors.affiliation: Lumera Corporation, Bothell, WA 98011, USA.
authors.forename: Christina
authors.initials: C
authors.lastname: Boozer
authors.forename: Gibum
authors.initials: G
authors.lastname: Kim
authors.forename: Shuxin
authors.initials: S
authors.lastname: Cong
authors.forename: Hannwen
authors.initials: H
authors.lastname: Guan
authors.forename: Timothy
authors.initials: T
authors.lastname: Londergan
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D004353:Drug Evaluation, Preclinical; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods; D011485:Protein Binding / Q000502:physiology; D011506:Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D011506:Proteins