title: Efficient high-throughput discovery of large peptidic hormones and biomarkers.
abstract: A novel approach is presented for the simultaneous identification and relative quantification of secreted peptides, particularly those that have been historically difficult to analyze in a concerted manner. Peptides exceeding 60 residues with various degrees of post-translational modification were identified on a liquid chromatographic time scale. The approach demonstrates high efficiency pattern-based recognition analysis of complex neuroendocrine peptide sets and enables rapid identification of biomarkers from biological material.
authors.affiliation: Amylin Pharmaceuticals, Inc., 9360 Towne Centre Dr., San Diego California 92121, USA. staylor@amylin.com
authors.forename: Steven W
authors.initials: SW
authors.lastname: Taylor
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Andon
authors.forename: James M
authors.initials: JM
authors.lastname: Bilakovics
authors.forename: Carolyn
authors.initials: C
authors.lastname: Lowe
authors.forename: Michael R
authors.initials: MR
authors.lastname: Hanley
authors.forename: Richard
authors.initials: R
authors.lastname: Pittner
authors.forename: Soumitra S
authors.initials: SS
authors.lastname: Ghosh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D005576:Colforsin / Q000494:pharmacology; D003198:Computer Simulation; D017077:Culture Media, Conditioned / Q000737:chemistry; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006728:Hormones / Q000737:chemistry*; D007340:Insulinoma / Q000737:chemistry* / Q000473:pathology; D008969:Molecular Sequence Data; D010190:Pancreatic Neoplasms / Q000737:chemistry* / Q000473:pathology; D010455:Peptides / Q000737:chemistry*; D040901:Proteomics / Q000379:methods; D051381:Rats
chemical_list: D014408:Biomarkers, Tumor; D017077:Culture Media, Conditioned; D006728:Hormones; D010455:Peptides; D005576:Colforsin