title: Inhibitory effects of Agaricus blazei on mast cell-mediated anaphylaxis-like reactions.
abstract: Agaricus blazei is a medicinal mushroom native to Brazil. It used to be a source of anti-tumor and immunmoactive compounds and considered a health food in many countries. However, its specific effect against mast cell-mediated anaphylactic reactions is still unknown. In the present study, we investigated the effect of Agaricus blazei water extract (ABWE) on mast cell-mediated anaphylaxis-like reactions. We examined whether ABWE could inhibit systemic anaphylaxis-like reaction, ear swelling response, passive cutaneous anaphylaxis, and mast cell activation. ABWE inhibited compound 48/80-induced systemic anaphylaxis-like reaction, ear swelling response, and passive cutaneous anaphylaxis-like reaction in mice. ABWE also inhibited anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. ABWE dose-dependently inhibited compound 48/80-induced or anti-DNP IgE-mediated histamine release from rat peritoneal mast cells (RPMC). Moreover, pretreatment with ABWE reduced compound 48/80-induced calcium uptake into RPMC. When ABWE was added, the level of intracellular cAMP in RPMC showed a significant increase compared with that of control cells. In addition, ABWE significantly inhibited compound 48/80-induced cAMP reduction in RPMC. These results propose that ABWE may be beneficial in the treatment of mast cell-mediated anaphylactic reactions.
authors.affiliation: Department of Anatomy, Chonbuk National University Medical School, Jeonbuk, Republic of Kora.
authors.forename: Yun Ho
authors.initials: YH
authors.lastname: Choi
authors.forename: Guang Hai
authors.initials: GH
authors.lastname: Yan
authors.forename: Ok Hee
authors.initials: OH
authors.lastname: Chai
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lim
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Moo Sam
authors.initials: MS
authors.lastname: Lee
authors.forename: Eui-Hyeog
authors.initials: EH
authors.lastname: Han
authors.forename: Hyoung Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Song
mesh_terms: D000364:Agaricus; D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004423:Ear; D004487:Edema / Q000517:prevention & control; D006636:Histamine Release / Q000187:drug effects; D007073:Immunoglobulin E / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D010936:Plant Extracts; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D002118:Calcium