[Expression of CD44 and nm23-H1 protein in the primary and metastatic lymph node lesions in supraglottic and hypopharyngeal cancer].
OBJECTIVE
To study the metastasis feature of the primary and metastatic lymph node lesions in supraglottic or hypopharyngeal cancer.


METHODS
The expression of CD44 and nm23-H1 in specimens from the primary and metastatic lymph node lesions of the 41 cases with supraglottic or hypopharyngeal cancer were studied with immunohistochemistry method and flow cytometry.


RESULTS
No correlation was found between the expression of CD44, nm23-H1 and the tumor differentiation of the supraglottic or hypopharyngeal cancer, but their expression related with the clinical staging. The CD44 and nm23-H1 positive expression rates in the primary and metastatic lymph node lesions were 75.6% (31/41), 85.4% (35/41) and 34.1% (14/41), 26.8% (11/41) respectively (P >0.05). The average fluorescence index of CD44 and nm23-H1 in the primary and metastatic lymph node lesions were 1.27 +/- 0.18, 1.33 +/- 0.16 and 1.11 +/- 0.19, 1.08 +/- 0.15 (x +/- s) respectively (P >0.05).


CONCLUSIONS
The expressions of CD44 and nm23-H1 in the metastatic lymph node tumor had no difference compared with that in primary tumor of the supraglottic or hypopharyngeal cancer. The difference of metastasis potentials between the primary and metastatic lymph node lesions in the same patient was not proved in this study and should be further investigated from multiple oncogens markers.
Wang
Bao-shan
BS
Department of Otorhinolaryngology, First Affiliated Hospital, Hebei Medical University, Shijiazhuang, China. wangbaoshanent@yahoo.com.cn
Song
Dong-mei
DM
Ma
Jian-gang
JG
Duan
Hui-jun
HJ
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics*; D007012:Hypopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*; D009367:Neoplasm Staging
D018960:Hyaluronan Receptors; D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human