[Establishment of a novel preliminary screening model for bioactive parts of folium of Isatis indigotica based on biothermodynamics].
OBJECTIVE
To apply a novel method for screening the bioactive parts and substances of traditional Chinese medicines (TCM) based on biothermodynamic theories.


METHODS
The power-time curves of Escherchia coli proliferation at different concentrations of decoction and its five bioactive parts of folium of Isatis indigotica (FII) were determined by TAM Air Isothermal Calorimeter. The growth rate constants of promotive and inhibitory actions were calculated. Moreover, the potential pharmacological action of FII was analyzed from the point of view of TCM theory.


RESULTS
The growth rate constant k1 and Pm of the studied bacterium in the presence of the decoction of FII were concentration-dependant. The k1 and Pm increased with the mass increase of the decoction and its water-extract of FII. However, the value of k1 and Pm decreased with the increasing concentrations of its other four organic solvent parts. The experimental results revealed that the sequence of inhibitory activity of different extracts was n-BuOH extract > EtOAc extract > chloroform extract > PE extract.


CONCLUSIONS
The decoction and its water-extract of FII stimulated the metabolism of E. coli. While the other four organic solvent extracts of the total decoction inhibited the growth of tested bacterium. The microcalorimetric method has many virtues such as extensive applicability, convenience, etc, and provides a new and useful means for primary screening the bioactive parts and substances of TCM.
Wu
Yan-wen
YW
Institute of Chinese Medicine, 302 Hospital of PLA, Beijing.
Xiao
Xiao-he
XH
Gao
Wen-yuan
WY
Zhao
Yan-ling
YL
Jin
Cheng
C
Liu
Yi
Y
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002151:Calorimetry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D031219:Isatis / Q000737:chemistry*; D008826:Microbial Sensitivity Tests / Q000379:methods; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013816:Thermodynamics
D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal