Gingival crevicular fluid laminin-5 gamma2-chain levels in periodontal disease.
OBJECTIVE
Our study aimed to examine the molecular forms and gingival crevicular fluid (GCF) levels of laminin-5 gamma2-chain in patients with different periodontal disease, and compare the effects of P.gingivalis trypsin-like proteinase on intact laminin-5 gamma2-chain species.


METHODS
Eighteen patients with generalized aggressive periodontitis (G-AgP), 29 patients with chronic periodontitis (CP), 20 with gingivitis and 20 periodontally healthy subjects were included. Probing depth, clinical attachment loss, presence of bleeding on probing and plaque were recorded. Molecular forms and GCF laminin-5 gamma2-chain levels and the effects of P. gingivalis trypsin-like proteinase on intact laminin-5 gamma2-chain were analysed by computer-quantitated Western immunoblotting.


RESULTS
Laminin-5 gamma2-chain 40 and 70 kDa fragments could be detected in all groups, in varying levels. The CP group had elevated GCF laminin-5 gamma2-chain fragment levels compared with the gingivitis and healthy groups (p<0.008). The G-AgP group had GCF laminin-5 gamma2-chain fragment levels similar to the gingivitis and healthy groups (p>0.008). GCF laminin-5 gamma2-chain fragments differed clearly from the multiple lower molecular size fragments of P.gingivalis trypsin-laminin-5 gamma2-chain proteinases.


CONCLUSIONS
Increased GCF laminin-5 gamma2-chain fragments in periodontitis sites with deep periodontal pocket suggest that these cleaved 40 and 70 kDa fragments could reflect the extent of the inflammatory reaction in CP.
Emingil
Gülnur
G
Department of Periodontology, Ege University, School of Dentistry, Izmir, Turkey, and Department of Oral and Maxillofacial Diseases, Helsinki University Cnetral Hospital, Finland. gemingil@yahoo.com
Kuula
Heidi
H
Pirilä
Emma
E
Atilla
Gül
G
Sorsa
Timo
T
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D001426:Bacterial Proteins / Q000378:metabolism; D015153:Blotting, Western; D016022:Case-Control Studies; D002908:Chronic Disease; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005891:Gingivitis / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D017622:Periodontal Attachment Loss / Q000378:metabolism; D010512:Periodontal Index; D010518:Periodontitis / Q000378:metabolism*; D016966:Porphyromonas gingivalis / Q000201:enzymology; D018709:Statistics, Nonparametric; D014357:Trypsin / Q000378:metabolism
D001426:Bacterial Proteins; C400567:LAMC2 protein, human; D007797:Laminin; D010446:Peptide Fragments; D014357:Trypsin