title: Sequential uniform designs for fingerprints development of Ginkgo biloba extracts by capillary electrophoresis.
abstract: A sequential procedure for the method of development of fingerprints based on a uniform design approach has been described in this paper. The sequential uniform design is used to reach the global optimum for a separation. The procedure is illustrated through developing the fingerprint of a Ginkgo biloba extract by capillary electrophoresis (CE) with diode array detection (DAD). The local overlap index (LOVI) is proposed as a criterion to evaluate the separation quality of two-dimensional hyphenated data in the optimization process. The successful application of the described techniques, including a sequential procedure, the CE-DAD hyphenated method and the LOVI criterion, shows their practicality in the fingerprint development of herbal medicines. However, some critical remarks on their use can also be made.
authors.affiliation: Department of Analytical Chemistry and Pharmaceutical Technology, Pharmaceutical Institute, Vrije Universiteit Brussel-VUB, Laarbeeklaan 103, 1090 Brussels, Belgium.
authors.forename: Yi-Bing
authors.initials: YB
authors.lastname: Ji
authors.forename: Goedele
authors.initials: G
authors.lastname: Alaerts
authors.forename: Cheng-Jian
authors.initials: CJ
authors.lastname: Xu
authors.forename: Yu-Zhu
authors.initials: YZ
authors.lastname: Hu
authors.forename: Yvan
authors.initials: Y
authors.lastname: Vander Heyden
mesh_terms: D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D020441:Ginkgo biloba / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D002021:Buffers; D010936:Plant Extracts