Stability study of propoxur (Baygon) in whole blood and urine stored at varying temperature conditions.
A stability study has been initiated for propoxur (Baygon) in whole blood and urine samples stored over a period of 60 days at four different temperature conditions (room temperature, 4 degrees C, -20 degrees C, and -80 degrees C). Stability data was established on day 0, 1, 7, 14, 28, 42, and 60. Sample purification was done by solid-phase extraction using a weak cation exchange cartridge (Isolute CBA), and quantitation was carried out by a validated high-performance liquid chromatographic method with a photodiode-array UV detector. Propoxur was spiked at two different concentration levels in both blood and urine samples [low concentration (10 microg/L) and high concentration (100 microg/L)]. Isopropoxy phenol was observed as the major degradation product in blood and urine samples and confirmed by liquid chromatography-electrospray ionization-mass spectrometry. At room temperature, a substantial decrease in concentration of about 95% was observed at the end of the stability study in both blood and urine samples. However, at 4 degrees C, the concentration of propoxur observed after 60 days was around 60% in both samples. A decrease in temperature reduced the degradation, and finally propoxur was found to be stable at -80 degrees C and -20 degrees C for the whole observation period (60 days). The data collected suggests that knowledge about time-dependent decrease of propoxur in urine and blood samples is of considerable significance in forensic toxicology, and, therefore, forensic cases should be interpreted with caution.
Ramagiri
Suma
S
Osmania University, Department of Chemistry, Hyderabad-500007, India. sumakosanam@yahoo.com
Kosanam
Hari
H
Sai Prakash
P K
PK
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007306:Insecticides / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D001074:Propoxur / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D013048:Specimen Handling / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
D007306:Insecticides; D001074:Propoxur