title: Alternative isoform discrimination by the next generation of expression profiling microarrays.
abstract: Microarray expression profiling has revolutionised the way that many therapeutic targets have been identified over the past 10 years. High-density microarrays have allowed scientists to simultaneously scrutinise the expression of all genes encoded on a given genome. Although the data collected from classically designed microarrays greatly enriched the pool of information available to help guide the selection and design of new therapeutic strategies, they were unable to tell the complete story. The major limitation with most array designs is that they can only produce a global expression value for all transcripts produced from a specific locus and cannot monitor each individual alternative isoform produced from the interrogated locus. Recently, new array designs have been described, and become commercially available, that can efficiently monitor individual alternatively spliced isoforms produced from a single locus, allowing the research community to get a more accurate picture of the biological landscape of the expressed transcripts.
authors.affiliation: ExonHit Therapeutics, Inc., 217 Perry Parkway, Bdg 5, Gaithersburg, MD 20877, USA.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Pando
authors.forename: Vinayaka
authors.initials: V
authors.lastname: Kotraiah
authors.forename: Kevin
authors.initials: K
authors.lastname: McGowan
authors.forename: Laurent
authors.initials: L
authors.lastname: Bracco
authors.forename: Richard
authors.initials: R
authors.lastname: Einstein
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020033:Protein Isoforms / Q000235:genetics*
chemical_list: D020033:Protein Isoforms