[Spectroscopy research on cholesterol in hypercholesterolemia serum].
The cholesterol with different concentration in hypercholesterolemia serum was studied by the method of spectroscopy technology. The absorption and fluorescence spectra of normal human serum and hypercholesterolemia serum were obtained respectively; the spectral characteristic of each sample and the difference between two kinds of samples were discussed too. The results indicate that the absorption and fluorescence spectra of hypercholesterolemia serum are different from those of normal human serum. The absorptivity and the fluorescence intensity of hypercholesterolemia serum are both higher than those of normal human serum. Besides, there are new absorptive peaks and new fluorescence peaks in the spectrogram. Thus, the abnormalism of cholesterol in serum can be judged by comparing the absorption and fluorescence spectra. The researches in the present paper provide an experimental foundation for the diagnosis of cholesterol in blood.
Lan
Xiu-feng
XF
Department of Information Physics and Engineering, Nanjing University of Science and Technology, Nanjing, China.
Liu
Jiag-gang
JG
Liu
Yin
Y
Luo
Xiao-sen
XS
Tang
Xin-guo
XG
Lu
Shi-yue
SY
Ni
Xiao-wu
XW
D001774:Blood Chemical Analysis; D002784:Cholesterol / Q000097:blood / Q000737:chemistry*; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000175:diagnosis*; D013053:Spectrophotometry / Q000379:methods*
D002784:Cholesterol