Cytotoxic, antioxidant and antibacterial activities of Varthemia iphionoides Boiss. extracts.
The hexane, ethyl acetate, chloroform, ethanol and water extracts of aerial parts of Varthemia, Varthemia iphionoides, were investigated for cytotoxic activity against human myelocytic leukemia (HL-60) cells; DPPH radical-scavenging activity; antioxidative activity in the linoleic acid system; reducing power; antibacterial activity; the contents of phenolic compounds. A pronounced cytotoxic effect on human leukemia (HL-60) cells was shown in the hexane, chloroform and ethanol extracts, with inhibition of 89.0, 68.4 and 62.3%, respectively, at a concentration of 200 microg extract/ml. High DPPH radical-scavenging activity, antioxidative activity in the linoleic acid system and reducing power were found in the water and ethanol extracts, and were correlated to the contents of phenolic compounds. Antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus, Escherichia coli, Bacillus cereus and Salmonella enteritides was shown in the ethyl acetate and chloroform extracts. A compound responsible for the antibacterial activity was isolated from the ethyl acetate extract, and identified as 3-oxocostusic acid.
Al-Dabbas
Maher M
MM
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Suganuma
Toshihiko
T
Kitahara
Kanefumi
K
Hou
De-Xing
DX
Fujii
Makoto
M
D000085:Acetates; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018922:HL-60 Cells; D006586:Hexanes; D006801:Humans; D007597:Jordan; D008519:Medicine, Traditional; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045787:Sesquiterpenes, Eudesmane / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
C501027:3-oxocostusic acid; D000085:Acetates; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D003603:Cytotoxins; D005419:Flavonoids; D044948:Flavonols; D006586:Hexanes; D010636:Phenols; D010936:Plant Extracts; D012717:Sesquiterpenes; D045787:Sesquiterpenes, Eudesmane; D000431:Ethanol; C007650:ethyl acetate