title: Phenotypic and genetic characterization of vancomycin-resistant enterococci from hospitalized humans and from poultry in Korea.
abstract: Vancomycin resistant enterococci (VRE) isolates from humans (23 isolates) and poultry (20 isolates) were characterized by antibiotic susceptibility, vancomycin resistance transferability, pulsed-field gel electrophoresis (PFGE), and structural analysis of Tn1546-like elements. VRE isolates from humans and poultry showed different resistance patterns, transferability, and transfer rate. In addition to these phenotypic differences between humans and poultry VRE, PFGE and the structure of Tn1546-like elements were also distinct. Most poultry isolates (16/20) were identical to the prototype vanA transposon, Tn1546, while most human isolates (21/23) had multiple integrations of insertion sequence. The transmission of VRE and vancomycin resistance determinant between humans and poultry could not be demonstrated in this study.
authors.affiliation: Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
authors.forename: Woo Kyung
authors.initials: WK
authors.lastname: Jung
authors.forename: Soon Keun
authors.initials: SK
authors.lastname: Hong
authors.forename: Ji Youn
authors.initials: JY
authors.lastname: Lim
authors.forename: Suk Kyung
authors.initials: SK
authors.lastname: Lim
authors.forename: Nam Hoon
authors.initials: NH
authors.lastname: Kwon
authors.forename: Jun Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Hye Cheong
authors.initials: HC
authors.lastname: Koo
authors.forename: So Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Keun Seok
authors.initials: KS
authors.lastname: Seo
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Ike
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanimoto
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Park
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004251:DNA Transposable Elements; D016521:Electrophoresis, Gel, Pulsed-Field; D016983:Enterococcus / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology* / Q000662:veterinary; D006760:Hospitalization; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010641:Phenotype; D011200:Poultry / Q000382:microbiology*; D011201:Poultry Diseases / Q000382:microbiology; D017422:Sequence Analysis, DNA; D020713:Vancomycin Resistance / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements