title: Hyper-thermostability of CutA1 protein, with a denaturation temperature of nearly 150 degrees C.
abstract: We found that the CutA1 protein, from Pyrococcus horikoshii (PhCutA1), has an extremely high denaturation temperature (T(d)) of nearly 150 degrees C, which exceeds the highest record determined by DSC by about 30 degrees C. To elucidate the mechanism of the ultra-high stability of PhCutA1, we analyzed the crystal structures of CutA1 proteins from three different sources, P. horikoshii, Thermus thermophilus, and Escherichia coli, with different growth temperatures (98, 75, and 37 degrees C). This analysis revealed that the remarkably increased number of ion pairs in the monomeric structure contributes to the stabilization of the trimeric structure and plays an important role in enhancing the T(d), up to 150 degrees C, for PhCutA1.
authors.affiliation: RIKEN SPring-8 Center, Harima Institute, Kouto, Sayo, Hyogo 679-5148, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Masahide
authors.initials: M
authors.lastname: Sawano
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ogasahara
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Sakaguchi
authors.forename: Bagautdin
authors.initials: B
authors.lastname: Bagautdinov
authors.forename: Etsuko
authors.initials: E
authors.lastname: Katoh
authors.forename: Chizu
authors.initials: C
authors.lastname: Kuroishi
authors.forename: Akeo
authors.initials: A
authors.lastname: Shinkai
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Katsuhide
authors.initials: K
authors.lastname: Yutani
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D006358:Hot Temperature; D011489:Protein Denaturation; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D043902:Pyrococcus horikoshii / Q000737:chemistry*; D040681:Structural Homology, Protein; D016963:Thermus thermophilus / Q000737:chemistry*
chemical_list: D019843:Archaeal Proteins; C094846:CutA protein, E coli; D029968:Escherichia coli Proteins