title: A porous poly(acrylonitrile-co-acrylic acid) film-based glucose biosensor constructed by electrochemical entrapment.
abstract: A novel glucose biosensor was constructed by electrochemical entrapment of glucose oxidase (GOD) into porous poly(acrylonitrile-co-acrylic acid), which was synthesized via radical polymerization of acrylonitrile and acrylic acid. The obtained biosensor showed a better stability and higher sensitivity than the biosensor prepared by simple physical adsorption. Effects of some experimental variables such as immobilization time, enzyme concentration, pH, applied potential, and temperature on the amperometric response of the sensor were investigated. The biosensor exhibited a rapid response to glucose (< 30s) with a linear range of 5 x 10(-6) to 3 x 10(-3)M and a sensitivity of 6.82 mAM(-1)cm(-2). The apparent Michaelis-Menten constant (K(M)(app)) was 7.3mM.
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: He
authors.forename: Shanxia
authors.initials: S
authors.lastname: Wang
authors.forename: Huaiguo
authors.initials: H
authors.lastname: Xue
authors.forename: Hao
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000179:Acrylates / Q000138:chemical synthesis / Q000737:chemistry*; D000181:Acrylonitrile / Q000138:chemical synthesis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000179:Acrylates; D004800:Enzymes, Immobilized; D011108:Polymers; D005949:Glucose Oxidase; D005947:Glucose; C036658:acrylic acid; D000181:Acrylonitrile