title: Construction, database integration, and application of an Oenothera EST library.
abstract: Coevolution of cellular genetic compartments is a fundamental aspect in eukaryotic genome evolution that becomes apparent in serious developmental disturbances after interspecific organelle exchanges. The genus Oenothera represents a unique, at present the only available, resource to study the role of the compartmentalized plant genome in diversification of populations and speciation processes. An integrated approach involving cDNA cloning, EST sequencing, and bioinformatic data mining was chosen using Oenothera elata with the genetic constitution nuclear genome AA with plastome type I. The Gene Ontology system grouped 1621 unique gene products into 17 different functional categories. Application of arrays generated from a selected fraction of ESTs revealed significantly differing expression profiles among closely related Oenothera species possessing the potential to generate fertile and incompatible plastid/nuclear hybrids (hybrid bleaching). Furthermore, the EST library provides a valuable source of PCR-based polymorphic molecular markers that are instrumental for genotyping and molecular mapping approaches.
authors.affiliation: Department Biologie I, Botanik, Ludwig-Maximilians-Universität München, Menzinger Strasse 67, 80638 München, Germany.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Mrácek
authors.forename: Stephan
authors.initials: S
authors.lastname: Greiner
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Cho
authors.forename: Uwe
authors.initials: U
authors.lastname: Rauwolf
authors.forename: Martha
authors.initials: M
authors.lastname: Braun
authors.forename: Pavan
authors.initials: P
authors.lastname: Umate
authors.forename: Johannes
authors.initials: J
authors.lastname: Altstätter
authors.forename: Rhea
authors.initials: R
authors.lastname: Stoppel
authors.forename: Lada
authors.initials: L
authors.lastname: Mlcochová
authors.forename: Martina V
authors.initials: MV
authors.lastname: Silber
authors.forename: Stefanie M
authors.initials: SM
authors.lastname: Volz
authors.forename: Sarah
authors.initials: S
authors.lastname: White
authors.forename: Renate
authors.initials: R
authors.lastname: Selmeier
authors.forename: Stephen
authors.initials: S
authors.lastname: Rudd
authors.forename: Reinhold G
authors.initials: RG
authors.lastname: Herrmann
authors.forename: Jörg
authors.initials: J
authors.lastname: Meurer
mesh_terms: D002467:Cell Nucleus / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods; D020224:Expressed Sequence Tags; D015723:Gene Library; D005819:Genetic Markers / Q000235:genetics; D031664:Oenothera / Q000235:genetics*; D051479:Plant Infertility / Q000235:genetics; D018087:Plastids / Q000235:genetics
chemical_list: D005819:Genetic Markers