Biodesulfurization in biphasic systems containing organic solvents.
Biphasic systems can overcome the problem of low productivity in conventional media and have been exploited for biocatalysis. Solvent-tolerant microorganisms are useful in biotransformation with whole cells in biphasic reactions. A solvent-tolerant desulfurizing bacterium, Pseudomonas putida A4, was constructed by introducing the biodesulfurizing gene cluster dszABCD, which was from Rhodococcus erythropolis XP, into the solvent-tolerant strain P. putida Idaho. Biphasic reactions were performed to investigate the desulfurization of various sulfur-containing heterocyclic compounds in the presence of various organic solvents. P. putida A4 had the same substrate range as R. erythropolis XP and could degrade dibenzothiophene at a specific rate of 1.29 mM g (dry weight) of cells(-1) h(-1) for the first 2 h in the presence of 10% (vol/vol) p-xylene. P. putida A4 was also able to degrade dibenzothiophene in the presence of many other organic solvents at a concentration of 10% (vol/vol). This study is a significant step in the exploration of the biotechnological potential of novel biocatalysts for developing an efficient biodesulfurization process in biphasic reaction mixtures containing toxic organic solvents.
Tao
Fei
F
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Yu
Bo
B
Xu
Ping
P
Ma
Cui Qing
CQ
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D008969:Molecular Sequence Data; D005810:Multigene Family; D009930:Organic Chemicals / Q000494:pharmacology*; D016958:Pseudomonas putida / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012240:Rhodococcus / Q000235:genetics; D017422:Sequence Analysis, DNA; D012997:Solvents / Q000494:pharmacology*; D013457:Sulfur Compounds / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*; D014992:Xylenes / Q000494:pharmacology
D001426:Bacterial Proteins; D003470:Culture Media; D009930:Organic Chemicals; D012997:Solvents; D013457:Sulfur Compounds; D013876:Thiophenes; D014992:Xylenes; C016366:dibenzothiophene