Isoelectric focusing sample injection for capillary zone electrophoresis in a fused silica capillary.
An improvement has been made to couple isoelectric focusing (IEF) sample injection and capillary zone electrophoresis in an untreated fused silica capillary. Electroosmotic flow is efficiently prevented by simply using a rubber block at the outlet end of the capillary during IEF sample injection. The experimental conditions that affect the concentration effect are discussed. A concentration enhancement factor of over 100-fold can be easily obtained for two model proteins: lysozyme and ribonuclease A.
Zhang
Luo-Hong
LH
Department of Materials Science and Engineering, Faculty of Engineering, University of Fukui, Bunkyo 3-9-1. Fukui 910-8507, Japan.
Zhang
Cheng-Jie
CJ
Wu
Xing-Zheng
XZ
D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D009113:Muramidase / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
D012822:Silicon Dioxide; D012259:Ribonuclease, Pancreatic; D009113:Muramidase