Polymorphism of the C-reactive protein (CRP) gene is related to serum CRP Level and arterial pulse wave velocity in healthy elderly Japanese.
The objective of this study was to clarify relationships between the C-reactive protein (CRP) gene and both the serum level of CRP and arterial pulse wave velocity (PWV), using haplotype analysis of healthy elderly Japanese. Five single-nucleotide polymorphisms (SNPs) of the human CRP gene (rs1341665, rs3091244, rs1800947, rs1130864 and rs1205) were used to genotype 315 healthy elderly Japanese subjects (mean age, 77.9 +/- 4.1 years; male/female ratio, 0.96). Linkage disequilibrium was analyzed for the five SNPs. The frequency of each haplotype and diplotype was estimated using the expectation/maximization (EM) algorithm. There were statistically significant associations between the CRP level and two CRP genotypes; the p value for the T allele of rs3091244 (CT + AT + TT vs. CC + CA + AA) was 0.002 (95% confidential interval [CI], 2.1-24), and the p value for the T allele of rs1130864 (TT + TC vs. CC) was 0.002 (95% CI, 2.1-24). The only genotype that was significantly associated with arterial PWV was the C allele of rs1800947, with a p value of 0.039. The haplotype was constructed using rs1341665, rs3091244 and rs1800947, in that order. There was a significant association between the CRP level and the T-T-G haplotype, with a p value of 0.002 (95% CI, 2.1-24). There was a significant association between arterial PWV and the C-C-C haplotype, with a p value of 0.039. We concluded that rs3091244, rs1130864 and the T-T-G haplotype are genetic markers for elevated basal CRP levels. rs1800947 and the C-C-C haplotype appear to be susceptibility markers for atherosclerosis, but this requires confirmation.
Morita
Akihiko
A
Division of Neurology, Department of Medicine, Nihon University School of Medicine, Tokyo, Japan.
Nakayama
Tomohiro
T
Doba
Nobutaka
N
Hinohara
Shigeaki
S
Soma
Masayoshi
M
D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D000483:Alleles; D001161:Arteriosclerosis / Q000097:blood / Q000235:genetics / Q000503:physiopathology; D001794:Blood Pressure / Q000235:genetics* / Q000502:physiology; D002097:C-Reactive Protein / Q000032:analysis* / Q000235:genetics* / Q000502:physiology; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D006973:Hypertension / Q000235:genetics / Q000503:physiopathology; D007564:Japan; D015810:Linkage Disequilibrium; D008297:Male; D020641:Polymorphism, Single Nucleotide
D005819:Genetic Markers; D002097:C-Reactive Protein