title: Binding studies for serotoninergic, dopaminergic and noradrenergic receptors of Valeriana adscendens Trel. extracts.
abstract: Valeriana adscendens Trel. (Valerianaceae) is a psychoactive plant usually used in magical-therapeutic rituals in traditional practices of the Northern Peruvian Andes. Previous studies have been carried out on extracts of aerial parts in order to validate its traditional use. The results indicated that Valeriana adscendens exerts important effects on the central nervous system. Aim of the present study is to evaluate if the effects on the central nervous system of Valeriana adscendens extracts can be associated with interaction with some CNS receptors. In this work we examined affinity and selectivity of two Valeriana adscendens extracts (methanolic and aqueous) towards 5-HT(1A), 5-HT(2A), 5-HT(2C) serotononergic, D(1) and D(2) dopaminergic, alpha(1) and alpha(2) noradrenergic receptors by a preliminary binding screen. The results show weak affinity to 5-HT(1A) for the aqueous extract. Both extracts showed affinity for D(1) receptors, but only for the methanolic extract the IC(50) value was determinable (30.14 microg/ml). No affinity for 5-HT(2A), 5-HT(2C) serotononergic receptors, alpha(1) and alpha(2) noradrenergic receptors and D(2) receptors was recorded for the extracts.
authors.affiliation: Dipartimento di Farmacologia Giorgio Segre, Università degli Studi di Siena, via delle Scotte 6, 53100 Siena, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Nencini
authors.forename: Federica
authors.initials: F
authors.lastname: Cavallo
authors.forename: Anna
authors.initials: A
authors.lastname: Capasso
authors.forename: Vincenzo
authors.initials: V
authors.lastname: De Feo
authors.forename: Laura
authors.initials: L
authors.lastname: De Martino
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Bruni
authors.forename: Giorgio
authors.initials: G
authors.lastname: Giorgi
authors.forename: Lucia
authors.initials: L
authors.lastname: Micheli
mesh_terms: D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D001667:Binding, Competitive / Q000187:drug effects; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D018492:Dopamine Antagonists / Q000494:pharmacology; D008297:Male; D000432:Methanol; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D011869:Radioligand Assay / Q000379:methods; D051381:Rats; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism; D044402:Receptor, Serotonin, 5-HT2A / Q000378:metabolism; D044405:Receptor, Serotonin, 5-HT2C / Q000378:metabolism; D011941:Receptors, Adrenergic / Q000378:metabolism*; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism; D018341:Receptors, Adrenergic, alpha-2 / Q000378:metabolism; D011954:Receptors, Dopamine / Q000378:metabolism*; D017447:Receptors, Dopamine D1 / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D011985:Receptors, Serotonin / Q000378:metabolism*; D014316:Tritium; D014632:Valerian / Q000737:chemistry*; D014867:Water
chemical_list: D001552:Benzazepines; D018492:Dopamine Antagonists; D010936:Plant Extracts; D044402:Receptor, Serotonin, 5-HT2A; D044405:Receptor, Serotonin, 5-HT2C; D011941:Receptors, Adrenergic; D018340:Receptors, Adrenergic, alpha-1; D018341:Receptors, Adrenergic, alpha-2; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D011985:Receptors, Serotonin; D014867:Water; D014316:Tritium; D044282:Receptor, Serotonin, 5-HT1A; D000432:Methanol