Acetate oxidation is the dominant methanogenic pathway from acetate in the absence of Methanosaetaceae.
The oxidation of acetate to hydrogen, and the subsequent conversion of hydrogen and carbon dioxide to methane, has been regarded largely as a niche mechanism occurring at high temperatures or under inhibitory conditions. In this study, 13 anaerobic reactors and sediment from a temperate anaerobic lake were surveyed for their dominant methanogenic population by using fluorescent in situ hybridization and for the degree of acetate oxidation relative to aceticlastic conversion by using radiolabeled [2-14C]acetate in batch incubations. When Methanosaetaceae were not present, acetate oxidation was the dominant methanogenic pathway. Aceticlastic conversion was observed only in the presence of Methanosaetaceae.
Karakashev
Dimitar
D
Institute of Environment & Resources DTU, Technical University of Denmark, Building 113, DK-2800 Kgs. Lyngby, Denmark.
Batstone
Damien J
DJ
Trably
Eric
E
Angelidaki
Irini
I
D000085:Acetates / Q000378:metabolism*; D000693:Anaerobiosis; D005618:Fresh Water / Q000382:microbiology*; D008697:Methane / Q000378:metabolism*; D019640:Methanosarcinales / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA
D000085:Acetates; D008697:Methane