Isolation and characterization of functional insertion sequences of rhizobia.
Rhizobia are a group of bacteria that form nodules on the roots of legume host plants. The sequenced genomes of the rhizobia are characterized by the presence of many putative insertion sequences (IS) elements. However, it is unknown whether these IS elements are functional and it is therefore relevant to assess their transposition activity. In this work, several functional insertion sequences belonging to the IS1256, IS3, IS5, IS166, and IS21 families were captured from Rhizobium tropici, Rhizobium sp. NGR234 and Sinorhizobium meliloti, using pGBG1 as a trapping system. In silico analysis shows that homologs of rhizobia mobile elements are present in distantly related genomes, suggesting that Rhizobium IS elements are prone to genetic transfer.
Hernandez-Lucas
Ismael
I
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. ismaelh@ibt.unam.mx
Ramirez-Trujillo
Jose Augusto
JA
Gaitan
Miguel Angel
MA
Guo
Xianwu
X
Flores
Margarita
M
Martinez-Romero
Esperanza
E
Perez-Rueda
Ernesto
E
Mavingui
Patrick
P
D019295:Computational Biology; D004251:DNA Transposable Elements; D016680:Genome, Bacterial; D012231:Rhizobium / Q000235:genetics*; D042162:Rhizobium tropici / Q000235:genetics*; D016962:Sinorhizobium meliloti / Q000235:genetics*
D004251:DNA Transposable Elements