title: [Effect of some active Chinese herbal fraction on brain tissue proteomic profile of ischemic mouse].
abstract: OBJECTIVE
  To study the effect of some active Chinese herbal fraction on protein expression of brain tissue in ischemic mouse with proteomic technique.
  
  
  METHODS
  Ischemia-reperfusion mice were treated with baicalin, geniposide, cholic acid and concha margaritifera respectively for 3 hrs, and then their brain tissue were taken to extract the total protein. Protein expression in ischemic mouse brain was analyzed with surface-enhanced laser desorption/inionation-time of flight-mass spectra (SELDI-TOF-MS) protein-chip.
  
  
  RESULTS
  The four components tested had effect on 3 target proteins at 5373Da, 5707Da and 15103Da, showing the nature of multi-target and with different action on protein expression.
  
  
  CONCLUSIONS
  Protein-chip is an effective approach for exploring the pharmacological mechanism of Chinese herbal fraction.
authors.affiliation: The People's Hospital of Changchun.
authors.forename: Yuan-ying
authors.initials: YY
authors.lastname: Song
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Xun
authors.initials: X
authors.lastname: Qu
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D020520:Brain Infarction / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use*; D005260:Female; D008297:Male; D051379:Mice; D008517:Phytotherapy; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal