title: Incorporation of a lauric acid-conjugated GRGDS peptide directly into the matrix of a poly(carbonate-urea)urethane polymer for use in cardiovascular bypass graft applications.
abstract: Gly-Arg-Gly-Asp-Ser (GRGDS) was modified by conjugation to lauric acid (LA) to facilitate incorporation into the matrix of a poly(carbonate-urea)urethane (PCU) used in vascular bypass grafts. GRGDS and LA-GRGDS were synthesized using solid phase Fmoc chemistry and characterized by high performance liquid chromatography and Fourier transform infrared spectroscopy. LA-GRGDS was passively coated and incorporated as nanoparticle dispersion on the PCU films. Biocompatibility of the modified surfaces was investigated. Endothelial cells seeded on LA-GRGDS coated and incorporated PCU showed after 48 h and 72 h a significant (p < 0.05) increase in metabolism compared with unmodified PCU. The platelet adhesion and hemolysis studies showed that the modification of PCU had no adverse effect. In conclusion, LA-conjugated RGD derivatives, such as LA-GRGDS, that permit solubility into solvents used in solvent casting methodologies should have wide applicability in polymer development for use in coronary, vascular, and dialysis bypass grafts, and furthermore scaffolds utilized for tissue regeneration and tissue engineering.
authors.affiliation: Biomaterial and Tissue Engineering Centre (BTEC), Academic Division of Surgery & Interventional Sciences, University College London, Hampstead Campus, London NW3 2PF, United Kingdom.
authors.forename: Asmeret G
authors.initials: AG
authors.lastname: Kidane
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Punshon
authors.forename: Henryk J
authors.initials: HJ
authors.lastname: Salacinski
authors.forename: Bala
authors.initials: B
authors.lastname: Ramesh
authors.forename: Audrey
authors.initials: A
authors.lastname: Dooley
authors.forename: Michael
authors.initials: M
authors.lastname: Olbrich
authors.forename: Johannes
authors.initials: J
authors.lastname: Heitz
authors.forename: George
authors.initials: G
authors.lastname: Hamilton
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Seifalian
mesh_terms: D001792:Blood Platelets / Q000187:drug effects; D002318:Cardiovascular Diseases / Q000601:surgery*; D002448:Cell Adhesion / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007850:Lauric Acids / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009204:Myocardial Revascularization; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D011108:Polymers / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D007850:Lauric Acids; D009842:Oligopeptides; D011108:Polymers; D011140:Polyurethanes; C110029:poly(carbonate urea) urethane; C030358:lauric acid; C049554:glycyl-arginyl-glycyl-aspartyl-serine