Characterization of bovine tau-preparations by two-dimensional gel electrophoresis.
The molecular diversity of bovine tau obtained by three different preparation protocols was characterized by immunoblot analyses after two-dimensional gel electrophoresis. These analyses revealed that tau was heterogeneously modified, that is, up to 20 spots separated along the pH gradient, mostly independent of the preparation protocol.
Volke
Daniela
D
Bioanalytics, Center for Biotechnology and Biomedicine, Faculty of Chemistry and Mineralogy, Leipzig University, Deutscher Platz 5, 04103 Leipzig, Germany.
Hoffmann
Ralf
R
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D016875:tau Proteins / Q000737:chemistry*
D016875:tau Proteins