title: [Construction of recombinant adenovirus vector carrying cell cycle controlling gene-p14ARF].
abstract: OBJECTIVE
  The recombinant adenovirus vector carrying p14ARF gene was constructed for using in the interference therapy in signal transduction of laryngeal squamous cell carcinoma.
  
  
  METHODS
  The total cDNA fragment of p14ARF was cloned into the shuttle plasmid pAdTrack-CMV, with the resultant plasmid and the backbone plasmid pAdEasy-1, the homologous recombination took place in the E.Coli BJ5183 and the recombinant adenoviral plasmid was generated. The adenoviruses were packaged and amplified in the 293 cells. Then the viral titer was checked by GFP.
  
  
  RESULTS
  The recombinant adenovirus vector carrying p14ARF was constructed successfully. The viral titer was 2.3 x 10(9).
  
  
  CONCLUSIONS
  The recombinant adenovirus vector could introduce p14ARF gene into the laryngeal squamous cell carcinoma line or tumor tissue effectively, which would provide experimental basis for the mechanisms and further study of the interference therapy in signal transduction of laryngeal squamous cell carcinoma.
authors.affiliation: Dept. of Human Anatomy, Institute of Preclinical Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Jun-Ming
authors.initials: JM
authors.lastname: Xian
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Zhou
authors.forename: Chuan-Yu
authors.initials: CY
authors.lastname: Liang
authors.forename: Shi-Xi
authors.initials: SX
authors.lastname: Liu
mesh_terms: D000256:Adenoviridae; D002453:Cell Cycle; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D011995:Recombination, Genetic; D025803:Tumor Suppressor Protein p14ARF
chemical_list: D025803:Tumor Suppressor Protein p14ARF