Formation of gut-like structures in vitro from mouse embryonic stem cells.
Embryonic stem (ES) cells have the potential to differentiate into all cell types originating from the three germ layers; however, there are still few reports about the formation of functional organs from embryonic stem cells. Recently, we reported that by hanging drops of mouse ES cells, embryoid bodies (EBs) formed gut-like structures in vitro composed of three layers corresponding to the epithelium, lamina propria, and musculature. The morphological features and the process of formation are similar to gut and its organogenesis in vivo. Thus, this is a good model for development of the gut and a useful tool for analysis of the factors required for gut organogenesis. The protocol basically involves a method of hanging drops to make EBs, which are then plated on coated dishes for outgrowth. EBs develop to form gut-like structures when induced to spontaneously enter a program of differentiation in vitro without addition of any extrinsic factors.
Torihashi
Shigeko
S
Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Japan.
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000033:anatomy & histology; D004628:Embryonic Structures / Q000648:ultrastructure; D041981:Gastrointestinal Tract / Q000166:cytology / Q000196:embryology; D051379:Mice; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*