Mangifera indica L. extract protects T cells from activation-induced cell death.
The aqueous stem bark extract of Mangifera indica L. (Vimang) has been reported to have antioxidant properties. AIDS is characterized by up-regulation of CD95 ligand (CD95L) expression and enhancement of activation-induced cell death (AICD). Recent studies demonstrate oxidative signals combined with simultaneous calcium (Ca(2+)) influx into the cytosol are required for induction of CD95L expression. In this study we show that M. indica extract attenuated anti-CD3-induced accumulation of reactive oxygen species (ROS) and intracellular free Ca(2+) and consequently, downregulates CD95L mRNA expression and CD95-mediated AICD. In addition, TCR triggering caused an elevation in the antioxidant enzyme manganous superoxide dismutase (Mn-SOD) and the increase in c-Jun N-terminal kinase (JNK) phosphorylation, both effects being prevented by M. indica extract. We provide a number of evidences regarding how M. indica extract enhance T-cell survival by inhibiting AICD, a finding associated with a decrease in oxidative stress generated through the TCR signaling pathway in activated T cells.
Hernández
Patricia
P
Department of Biomedical Research, Center of Pharmaceutical Chemistry, PO Box 16042, Atabey, Playa, Havana, Cuba. patricia.hdez@infomed.sld.cu
Delgado
Rene
R
Walczak
Henning
H
D017209:Apoptosis / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation / Q000276:immunology*; D031022:Mangifera / Q000737:chemistry* / Q000502:physiology; D010936:Plant Extracts / Q000737:chemistry; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
C501277:Mangifera indica extract; D010936:Plant Extracts