[Experimental study on protective effects of HupA in the treatment of isocarbophos poisoning].
OBJECTIVE
To investigate the therapeutic and prophylactic efficiency of HupA in mice with acute isocarbophos poisoning, and the protective effects of the HupA on AChE inhibited by isocarbophos.


METHODS
Mice were randomizedly divided into the non-treatment group, the atropine control group, the HupA treatment group and the atropine and HupA combined treatment group. Toxic signs and survival rates were observed and compared among these groups. The AChE activity was monitored in the whole blood, the red cells and brain tissue exposed to isocarbophos in the either treated with HupA or non-treated groups.


RESULTS
In HupA treatment group compared with the non-treatment group, toxic signs were significantly decreased and the survival rate was increased. The therapeutic efficiency in the atropine and HupA combined treatment group was better than other groups. After isocarbophos was administered, the AChE activity in the HupA treatment group and the non-treatment group was decreased. However, the AChE activity in the whole blood (1.096 +/- 0.111), (1.262 +/- 0.146), (1.181 +/- 0.353) U/ml, the red cells (0.798 +/- 0.063), (1.000 +/- 0.176), (0.837 +/- 0.331) and the brain tissue (13.739 +/- 2.970), (18.507 +/- 3.466), (10.764 +/- 2.212) U/g in HupA treatment group 0.5, 1 and 2 hours after isocarbophos was administered was significantly higher than those in the non-treatment group (P < 0.05 or P < 0.01).


CONCLUSIONS
HupA has therapeutic effect on mice with acute isocarbophos poisoning. The protective effect of HupA on blood and brain AChE inhibited by isocarbophos may be one of the mechanisms of the therapeutic effect of HupA in acute Isocarbophos poisoning.
Liu
Li
L
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Xie
Guang-yun
GY
Wang
Jian
J
Sun
Jin-xiu
JX
D000110:Acetylcholinesterase / Q000097:blood / Q000378:metabolism; D000470:Alkaloids; D000818:Animals; D001921:Brain / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000627:therapeutic use*; D007306:Insecticides / Q000506:poisoning*; D008294:Malathion / Q000506:poisoning*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011041:Poisoning / Q000188:drug therapy; D011897:Random Allocation; D012717:Sesquiterpenes / Q000627:therapeutic use*
D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D007306:Insecticides; D012717:Sesquiterpenes; C050426:huperzine A; D000110:Acetylcholinesterase; D008294:Malathion