title: Antibody responses induced by long-term vaccination with an octovalent conjugate Pseudomonas aeruginosa vaccine in children with cystic fibrosis.
abstract: We assessed the serological responses over 10 years to repeated immunization of cystic fibrosis (CF) patients with an O-polysaccharide (OPS)-toxin A conjugate vaccine against Pseudomonas aeruginosa. A retrospective analysis was performed with sera from 25 vaccinated and 25 unvaccinated children treated at the same CF centre and matched for clinical management, age and gender. Yearly immunization led to sustained elevations of serum immunoglobulin G (IgG) antibody levels to all vaccine components. Eighteen unvaccinated patients but only eight vaccinated ones developed chronic pseudomonal lung infections. Infection rapidly caused further marked elevations of polysaccharide- but not toxin A-specific serum IgG in both immunized and nonimmunized patients, indicating that protection did not depend on the quantity of IgG present. However, qualitative analyses revealed that the protective capacity of specific serum IgG antibodies was linked to high affinity and to specificity for OPS serotypes rather than for lipopolysaccharide core epitopes.
authors.affiliation: Berna Biotech Ltd, Research Immunology, Bern, Switzerland.
authors.forename: Adrian W
authors.initials: AW
authors.lastname: Zuercher
authors.forename: Michael P
authors.initials: MP
authors.lastname: Horn
authors.forename: John U
authors.initials: JU
authors.lastname: Que
authors.forename: Anna
authors.initials: A
authors.lastname: Ruedeberg
authors.forename: Martin H
authors.initials: MH
authors.lastname: Schoeni
authors.forename: Urs B
authors.initials: UB
authors.lastname: Schaad
authors.forename: Paul
authors.initials: P
authors.lastname: Marcus
authors.forename: Alois B
authors.initials: AB
authors.lastname: Lang
mesh_terms: D000293:Adolescent; D000328:Adult; D000915:Antibody Affinity / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology* / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D000939:Epitopes; D005098:Exotoxins / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007223:Infant; D008297:Male; D011552:Pseudomonas Infections / Q000276:immunology / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012189:Retrospective Studies
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; C068568:E coli O18 O-specific polysaccharide-toxin A; D000939:Epitopes; D005098:Exotoxins; D007074:Immunoglobulin G