title: Molecular and virological studies on a rare case of biliary papillomatosis.
abstract: In a recurrence of a rare case of biliary papillomatosis, a disease that often becomes malignant, the presence of human papillomavirus (HPV), Cytomegalovirus (CMV) and Epstein-Barr virus (EBV), as well as DNA ploidy and markers of proliferation and invasion, were examined. No such viruses were identified by polymerase chain reactions. Moreover, markers of invasion, such as laminin, and of proliferation, MIB1 and cyclin A, were absent or at normal levels despite progressive superficial growth of the tumour. The tumour was aneuploid, but the p53-p21 tumour growth suppressor system was not mutated. It was concluded that the presented case of tumour relapse, despite an anamnesis of seven years and its expanding but non-invasive growth, did not contain the viruses that were examined for, and had not become malignant.
authors.affiliation: Radiumhemmet Department of Oncology, Karolinska Institutet, Stockholm, Sweden. peter.gunven@karolinska.se
authors.forename: Peter
authors.initials: P
authors.lastname: Gunvén
authors.forename: Gert
authors.initials: G
authors.lastname: Auer
authors.forename: Ulf
authors.initials: U
authors.lastname: Gerdes
authors.forename: Hanna Mellin
authors.initials: HM
authors.lastname: Dahlstrand
authors.forename: Ilona
authors.initials: I
authors.lastname: Lewensohn-Fuchs
authors.forename: Tina
authors.initials: T
authors.lastname: Dalianis
mesh_terms: D001650:Bile Duct Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D019925:Cyclin A / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000821:virology; D010212:Papilloma / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics; D011003:Ploidies; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014412:Tumor Virus Infections / Q000150:complications / Q000235:genetics / Q000821:virology
chemical_list: C493956:CDKN1A protein, human; D019925:Cyclin A; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004279:DNA, Viral; D019394:Ki-67 Antigen; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53