Adverse effect of chloride impurities on lipase-catalyzed transesterifications in ionic liquids.
The adverse influence of chloride impurities on the lipase-catalyzed transesterification in ionic liquid is described. The activity of lipase from Rhizomucor miehei exponentially decreased with increasing Cl(-) content in 1-octyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl] amide, [Omim][Tf(2)N], and the activity of lipase in [Omim][Tf(2)N] mixture containing 2% [Omim] [Cl] was only about 2% of the activity in pure [Omim][Tf(2)N]. The activity of lipase from Candidantarctica linearly decreased at about 5% with every 1% increase in [Omim][Cl] with there being no activity in [Omim][Tf(2)N] containing about 20% [Omim][Cl].
Lee
Sang Hyun
SH
Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea.
Ha
Sung Ho
SH
Lee
Sun Bok
SB
Koo
Yoon-Mo
YM
D002384:Catalysis; D002712:Chlorides / Q000494:pharmacology*; D004795:Enzyme Stability; D004951:Esterification; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D020103:Rhizomucor / Q000201:enzymology*
D002712:Chlorides; D007093:Imidazoles; D052578:Ionic Liquids; D008049:Lipase