L-nitroimidazole ornithine limits exercise-induced increases in cell proliferation in the hippocampus of adult mice.
Voluntary exercise such as running induces a dramatic increase in adult stem cell proliferation within the dentate gyrus, and endothelial nitric oxide synthase helps regulate cell proliferation. The role of endothelial nitric oxide synthase in exercise-induced cell proliferation in the brain, however, has not been examined. In the present study, exercise for 1 week increased endothelial nitric oxide synthase and nicotinamide adenine dinucleotide phosphate-diaphorase immunoreactivity in the microvessels of the dentate gyrus. In addition, blocking endothelial nitric oxide synthase activity (via a daily injection of 20 mg/kg L-nitroimidazole ornithine) during exercise reduced the number of cells within the dentate gyrus that were immunoreactive for Ki-67 protein and doublecortin. This study provides the first evidence that endothelial nitric oxide synthase upregulation may modulate exercise-induced granule cell proliferation within the dentate gyrus.
Moon
Minho
M
Department of Anatomy, Medical Science and Engineering Research Center for Reactive Oxygen Species, College of Medicine, Kyunghee University, Seoul, Korea.
Huh
Youngbuhm
Y
Park
Chan
C
D000818:Animals; D002455:Cell Division; D018891:Dentate Gyrus / Q000166:cytology* / Q000187:drug effects; D004791:Enzyme Inhibitors; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000502:physiology; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009952:Ornithine / Q000031:analogs & derivatives* / Q000494:pharmacology; D010805:Physical Conditioning, Animal
D004791:Enzyme Inhibitors; D009569:Nitric Oxide; C065027:N(G)-iminoethylornithine; D009952:Ornithine; D052250:Nitric Oxide Synthase Type III