The Acetic Acid Tolerance Response induces cross-protection to salt stress in Salmonella typhimurium.
Salmonella typhimurium induces an Acid Tolerance Response (ATR) upon exposure to mildly acidic conditions in order to protect itself against severe acid shock. This response can also induce cross-protection to other stresses such as heat and salt. We investigated whether both the acetic acid induced and lactic acid induced ATR in S. typhimurium provided cross-protection to a salt stress at 20 degrees C. Acid-adapted cells were challenged with both a sodium chloride (NaCl) and potassium chloride (KCl) shock and their ability to survive ascertained. Acetic acid adaptation provided cells with protection against both NaCl and KCl stress. However, lactic acid adaptation did not protect against either osmotic stressor and rendered cells hypersensitive to NaCl. These results have implications for the food industry where hurdle technology means multiple sub-lethal stresses such as mild pH and low salt are commonly used in the preservation of products.
Greenacre
E J
EJ
Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK. elizabeth.greenacre@bbsrc.ac.uk
Brocklehurst
T F
TF
D019342:Acetic Acid / Q000494:pharmacology*; D000222:Adaptation, Physiological; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D005520:Food Preservatives / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D011189:Potassium Chloride / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology*
D005520:Food Preservatives; D012965:Sodium Chloride; D011189:Potassium Chloride; D019342:Acetic Acid