title: IFN-alpha/beta-dependent cross-priming induced by specific toll-like receptor agonists.
abstract: Toll-like receptors (TLR) are pattern recognition receptors that have been identified as crucial in the initiation of innate immune responses against pathogens. They are thought to be involved in shaping appropriate adaptive immune responses, although their precise contribution has not yet been fully characterised. Our aim was to investigate in vivo the effect of different TLR stimuli on cellular immune responses. We examined the ability of a range of TLR stimuli to induce CD8+ T cell responses against a model soluble protein antigen, ovalbumin (OVA). We found that TLR 3, TLR 4, and TLR 9 agonists induced functional cross-priming, and that this process was dependent on IFN-alpha/beta signalling pathway.
authors.affiliation: The Edward Jenner Institute for Vaccine Research, Compton RG20 7NN, UK. vanessa.durand@jenner.ac.uk
authors.forename: Vanessa
authors.initials: V
authors.lastname: Durand
authors.forename: Simon Y C
authors.initials: SY
authors.lastname: Wong
authors.forename: David F
authors.initials: DF
authors.lastname: Tough
authors.forename: Agnes
authors.initials: A
authors.lastname: Le Bon
mesh_terms: D000818:Animals; D016898:Interferon-alpha / Q000276:immunology*; D016899:Interferon-beta / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D051193:Toll-Like Receptors / Q000819:agonists / Q000276:immunology*
chemical_list: D016898:Interferon-alpha; D051193:Toll-Like Receptors; D016899:Interferon-beta; D010047:Ovalbumin