title: Structural studies of heterochiral DNA/DNA, RNA/RNA, and DNA/RNA duplexes.
abstract: Using DNA and RNA heptanucleotides containing an unnatural L-nucleotides as well as the complementary strands, effects of the introduction of an L-nucleotide on the structure of DNA/DNA, RNA/RNA, and DNA/RNA duplexes were investigated by circular dichroism experiments and RNase H-mediated RNA strand cleavage reaction. The results suggested that the substitution of the central D-nucleotide with an L-nucleotide in the duplexes causes the significant structural alterations as the duplex structures change to conformations with more B-form similarities.
authors.affiliation: Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. urata@gly.oups.ac.jp
authors.forename: Hidehito
authors.initials: H
authors.lastname: Urata
authors.forename: Hana
authors.initials: H
authors.lastname: Shimizu
authors.forename: Masao
authors.initials: M
authors.lastname: Akagi
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D013237:Stereoisomerism; D013816:Thermodynamics
chemical_list: D012313:RNA; D004247:DNA