title: Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae.
abstract: Large-scale protein overexpression phenotype screens provide an important complement to the more common gene knockout screens. Here, we have targeted the so far poorly understood Saccharomyces cerevisiae membrane proteome and report growth phenotypes for a strain collection overexpressing approximately 600 C-terminally tagged integral membrane proteins grown both under normal and three different stress conditions. Although overexpression of most membrane proteins reduce the growth rate in synthetic defined medium, we identify a large number of proteins that, when overexpressed, confer specific resistance to various stress conditions. Our data suggest that regulation of glycosylphosphatidylinositol anchor biosynthesis and the Na(+)/K(+) homeostasis system constitute major downstream targets of the yeast PKA/RAS pathway and point to a possible connection between the early secretory pathway and the cells' response to oxidative stress. We also have quantified the expression levels for >550 membrane proteins, facilitating the choice of well expressing proteins for future functional and structural studies.
authors.affiliation: Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.
authors.forename: Marie
authors.initials: M
authors.lastname: Osterberg
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
authors.forename: Jonas
authors.initials: J
authors.lastname: Warringer
authors.forename: Karin
authors.initials: K
authors.lastname: Melén
authors.forename: Anders
authors.initials: A
authors.lastname: Blomberg
authors.forename: Gunnar
authors.initials: G
authors.lastname: von Heijne
mesh_terms: D002110:Caffeine / Q000494:pharmacology; D016000:Cluster Analysis; D005656:Fungal Proteins / Q000737:chemistry; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry*; D018384:Oxidative Stress; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D012492:Salts / Q000494:pharmacology
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D012492:Salts; D049452:Green Fluorescent Proteins; D002110:Caffeine