16S rRNA phylogenetic investigation of the candidate division "Korarchaeota".
The environmental distribution and phylogeny of "Korarchaeota," a proposed ancient archaeal division, was investigated by using the 16S rRNA gene framework. Korarchaeota-specific primers were designed based on previously published sequences and used to screen a variety of environments. Korarchaeota 16S rRNA genes were amplified exclusively from high temperature Yellowstone National Park hot springs and a 9 degrees N East Pacific Rise deep-sea hydrothermal vent. Phylogenetic analyses of these and all available sequences suggest that Korarchaeota exhibit a high level of endemicity.
Auchtung
Thomas A
TA
Department of Organismic and Evolutionary Biology, Harvard University, Harvard University, Cambridge, MA 02138, USA.
Takacs-Vesbach
Cristina D
CD
Cavanaugh
Colleen M
CM
D019641:DNA, Archaeal / Q000032:analysis; D020459:Genes, rRNA; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D019615:Crenarchaeota / Q000145:classification* / Q000235:genetics
D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S