Isolation of mouse neuritic mRNAs.
Impaired local protein translation at postsynaptic sites has been hypothesized to be the cause of several neurological disorders such as fragile X syndrome, neurofibromatosis-1, Rett syndrome, and other syndromic and non-specific forms of mental retardation. Identification of which mRNAs are present in dendrites and the identification of the molecular pathways that they promote will be imperative to the understanding of the neuropathology of these diseases. Since mouse models are the most widely used animal models of human diseases we developed a cell culture based technique to isolate mRNAs from mouse neurites.
Reis
Surya A
SA
CBG-Department of Clinical Genetics, Erasmus MC, P.O. Box 1738, 3000, DR, Rotterdam, The Netherlands.
Oostra
Ben A
BA
Willemsen
Rob
R
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005600:Fragile X Syndrome / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D007834:Lasers; D051379:Mice; D042282:Microdissection; D016501:Neurites / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism
D012333:RNA, Messenger