title: Efficient expression of haloarchaeal nucleoside diphosphate kinase via strong porin promoter in moderately halophilic bacteria.
abstract: Enzymes from extremely halophilic archaea require high concentration of salts for their proper folding and consequently are expressed as an unfolded and inactive form in Escherichia coli. Moderate halophile, which accumulates protein stabilizers, i.e., compatible solutes, is an attractive host cell for the recombinant production of heterologous proteins, since such protein stabilizers may help folding of expressed proteins. Here, we succeeded in efficient expression and purification to homogeneity of recombinant haloarchaeal nucleoside diphosphate kinase (HsNDK) in moderate halophile using newly isolated strong porin promoter.
authors.affiliation: Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Chizuru
authors.initials: C
authors.lastname: Nagayoshi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tokunaga
authors.forename: Aya
authors.initials: A
authors.lastname: Hayashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Harazono
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hamasaki
authors.forename: Ayumi
authors.initials: A
authors.lastname: Ando
authors.forename: Masao
authors.initials: M
authors.lastname: Tokunaga
mesh_terms: D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D019617:Halobacterium salinarum / Q000201:enzymology / Q000235:genetics*; D009701:Nucleoside-Diphosphate Kinase / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D018272:Porins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D018272:Porins; D011994:Recombinant Proteins; D009701:Nucleoside-Diphosphate Kinase