title: Down-regulation of Jagged-1 induces cell growth inhibition and S phase arrest in prostate cancer cells.
abstract: Notch is an ancient cell signaling system that regulates cell fate specification, stem cell maintenance and initiation of differentiation in many tissues. It has been reported that Jagged-1, a Notch ligand, is significantly over expressed in metastatic prostate cancer compared to localized prostate cancer or benign prostatic tissues. Therefore, deregulation of Jagged-1 protein levels may play a role in prostate cancer cell growth and progression. Hence, the aim of our current study was to investigate the mechanistic role of Jagged-1 in prostate cancer cell growth and cell cycle progression. Our results show, for the first time, that down-regulation of Jagged-1 induces cell growth inhibition and S phase cell cycle arrest in prostate cancer cells, with reduced CDK2 kinase activity and increased p27 expression. These results suggest that Jagged-1 could be a potential therapeutic target for the treatment of prostate cancer.
authors.affiliation: Department of Biochemistry and Molecular Biology, Capital University of Medical Sciences, Beijing, China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.forename: Fakhara
authors.initials: F
authors.lastname: Ahmed
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Banerjee
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Li
authors.forename: Fazlul H
authors.initials: FH
authors.lastname: Sarkar
mesh_terms: D002135:Calcium-Binding Proteins / Q000235:genetics*; D002455:Cell Division; D045744:Cell Line, Tumor; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D000072100:Jagged-1 Protein; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000473:pathology*; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000072099:Serrate-Jagged Proteins
chemical_list: D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D034741:RNA, Small Interfering; D000072099:Serrate-Jagged Proteins; D051357:Cyclin-Dependent Kinase 2