title: Liver microcirculation after hepatic artery embolization with degradable starch microspheres in vivo.
abstract: OBJECTIVE
  To observe the dynamic changes of liver microcirculation in vivo after arterial embolization with degradable starch microspheres (DSM).
  
  
  METHODS
  DSM were injected into the proper hepatic artery through a silastic tube inserted retrogradely in gastroduodenal artery (GDA) of SD rats. Fluorescent microscopy was used to evaluate the dynamic changes of blood flow through the terminal portal venules (TPVs), sinusoids and terminal hepatic venules (THVs). The movements of DSM debris were also recorded. Six hours after injection of DSM, percentages of THVs with completely stagnant blood flow were recorded.
  
  
  RESULTS
  Two phases of blood flow change were recorded. In phase one: after intra-arterial injection of DSM, slow or stagnant blood flow was immediately recorded in TPVs, sinusoids and THVs. This change was reversible, and blood flow resumed completely. In phase two: after phase one, blood flow in TPVs changed again and three patterns of blood flow were recorded. Six hours after DSM injection, 36.9% +/- 9.2% of THVs were found with completely stagnant blood flow.
  
  
  CONCLUSIONS
  DSM can stop the microcirculatory blood flow in some areas of liver parenchyma. Liver parenchyma supplied by arteries with larger A-P shunt is considered at a higher risk of total microcirculatory blood stagnation after injection of DSM through hepatic artery.
authors.affiliation: Department of Interventional Radiology and Vascular Surgery, the First Hospital of Peking University, 100034 Beijing, China. vanjian@sohu.com
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Satoru
authors.initials: S
authors.lastname: Murata
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kumazaki
mesh_terms: D000818:Animals; D004621:Embolization, Therapeutic / Q000379:methods*; D006499:Hepatic Artery / Q000502:physiology*; D007269:Injections, Intra-Arterial; D008099:Liver / Q000098:blood supply* / Q000502:physiology; D008833:Microcirculation / Q000502:physiology*; D008856:Microscopy, Fluorescence; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000502:physiology; D013213:Starch / Q000008:administration & dosage*; D014699:Venules / Q000502:physiology
chemical_list: C083286:degradable starch microspheres; D013213:Starch