title: Development of a high performance liquid chromatographic method for systematic quantitative analysis of chemical constituents in rhubarb.
abstract: HPLC methods for the systematic determination of 30 compounds in Rhei Rhizoma (rhubarb) were developed. Using a combination of mobile phase gradient conditions and UV detection at 280 nm, all 30 compounds were separated satisfactorily with low detection limits (0.05-2 microg/ml). The developed methods provided a reliable calibration curve for each compound. By adopting these methods, the determination of 30 compounds in three kinds of rhubarb samples, derived from Rheum tanguticum, R. palmatum and R. officinale, was achieved. The constituent pattern of each rhubarb was clearly characterized through the quantitative composition of 30 major constituents of rhubarb.
authors.affiliation: Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama; 2630 Sugitani, Toyoma 930-0194, Japan. katsukok@ms.toyama-mpu.ac.jp
authors.forename: Katsuko
authors.initials: K
authors.lastname: Komatsu
authors.forename: Yorinobu
authors.initials: Y
authors.lastname: Nagayama
authors.forename: Ken
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
authors.forename: Purusotam
authors.initials: P
authors.lastname: Basnet
authors.forename: Meselhy Ragab
authors.initials: MR
authors.lastname: Meselhy
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D012250:Rheum / Q000737:chemistry*