Electrochemical biosensor evaluation of the interaction between DNA and metallo-drugs.
Electrochemical techniques were used to study the interaction between a panel of antiproliferative metallo-drugs and double-stranded DNA immobilized on screen-printed electrodes as a model of the analogous interaction occurring in solution. The propensity of a given metal drug to interact with DNA was measured as a function of the decrease of guanine oxidation signal, which was detected by square wave voltammetry. Estimates of variations in experimental parameters, such as the concentration of complexes, time following dissolution (ageing time) and the presence of chloride, are provided.
Mascini
Marco
M
Dipartimento di Chimica, Università di Firenze, Polo Scientifico Universitario, Via della Lastruccia 3, 50019, Sesto Fiorentino, FI, Italy.
Bagni
Graziana
G
Di Pietro
Maria Letizia
ML
Ravera
Mauro
M
Baracco
Sara
S
Osella
Domenico
D
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006147:Guanine / Q000737:chemistry; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000187:drug effects
D003432:Cross-Linking Reagents; D006147:Guanine; D004247:DNA; D002945:Cisplatin