title: Mutation-induced metabolite pool alterations in Corynebacterium glutamicum: towards the identification of nitrogen control signals.
abstract: The influence of glutamate dehydrogenase activity on nitrogen regulation in Corynebacterium glutamicum was investigated. As shown by RNA hybridization experiments deletion of the gdh gene results in a rearrangement of nitrogen metabolism. Even when sufficiently supplied with nitrogen sources, a gdh deletion strain showed the typical nitrogen starvation response of C. glutamicum. These changes in transcription correlate with distinct alterations of intracellular metabolite pattern. Metabolite analyses of different mutant strains and the wild type indicated that ammonium and 2-oxoglutarate might influence the nitrogen regulation system of C. glutamicum cells.
authors.affiliation: Institut für Biochemie, Universität zu Köln, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Müller
authors.forename: Julia
authors.initials: J
authors.lastname: Strösser
authors.forename: Sebastian
authors.initials: S
authors.lastname: Buchinger
authors.forename: Lars
authors.initials: L
authors.lastname: Nolden
authors.forename: Astrid
authors.initials: A
authors.lastname: Wirtz
authors.forename: Reinhard
authors.initials: R
authors.lastname: Krämer
authors.forename: Andreas
authors.initials: A
authors.lastname: Burkovski
mesh_terms: D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D005798:Genes, Bacterial; D005969:Glutamate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism*
chemical_list: D005969:Glutamate Dehydrogenase; D009584:Nitrogen