Generation and characterization of monoclonal antibodies against choline kinase alpha and their potential use as diagnostic tools in cancer.
Choline kinase alpha (ChoKalpha) is a metabolic enzyme involved in the synthesis of phosphatidylcholine, recently implicated in cancer onset since it is overexpressed in a variety of human cancers such as mammary, lung, colorectal and prostate adenocarcinomas. Furthermore, overexpression of ChoKalpha in human HEK293T cells confers them oncogenic properties with the induction of tumors after subcutaneous injection in nude mice. ChoKalpha levels in tumor samples have been analyzed using polyclonal antibodies and Western blotting. These techniques have considerable limitations and do not allow for a precise and efficient evaluation of the real significance of ChoK overexpression in human carcinogenesis. We developed a set of monoclonal antibodies with high specificity and sensitivity against ChoKalpha, and characterized their properties. We provide evidence that the newly generated MoAbs against ChoKalpha have potential use in cancer diagnosis by conventional immunohistochemistry techniques.
Gallego-Ortega
David
D
Translational Oncology Unit, CSIC-UAM-La Paz, Madrid, Spain.
Ramirez De Molina
Ana
A
Gutierrez
Ruth
R
Ramos
Maria Angeles
MA
Sarmentero
Jacinto
J
Cejas
Paloma
P
Nistal
Manuel
M
González Barón
Manuel
M
Lacal
Juan Carlos
JC
D000818:Animals; D000951:Antigens, Neoplasm / Q000737:chemistry*; D002460:Cell Line; D002797:Choline Kinase / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D025202:Molecular Diagnostic Techniques; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D014162:Transfection
D000951:Antigens, Neoplasm; D002797:Choline Kinase