Lack of telomerase activity in rabbit bone marrow stromal cells during differentiation along neural pathway.
OBJECTIVE
To investigate telomerase activity in rabbit bone marrow stromal cells (BMSCs) during their committed differentiation in vitro along neural pathway and the effect of glial cell line-derived neurotrophic factor (GDNF) on the expression of telomerase.


METHODS
BMSCs were acquired from rabbit marrow and divided into control group, GDNF (10 ng/ml) group. Cytokine.NSCs medium (prepared by our lab, Patent No. ZL02134314. 4) supplemented with 10 percent fetal bovine serum (FBS) was used to induce BMSCs differentiation along neural pathway. Fluorescent immunocytochemistry was employed to identify the expressions of Nestin, neuron-specific endase (NSE), and gial fibrillary acidic protein (GFAP). The growth curves of the cells and the status of cell cycles were analyzed, respectively. During the differentiation, telomerase activities were detected using the telomeric repeat amplification protocol-enzyme-linked immunosorbent assay (TRAP-ELISA).


RESULTS
BMSCs were successfully induced to differentiate along neural pathway and expressed specific markers of fetal neural epithelium, mature neuron and glial cells. Telomerase activities were undetectable in BMSCs during differentiation along neural pathway. Similar changes of cell growth curves, cell cycle status and telomerase expression were observed in the two groups.


CONCLUSIONS
Rabbit BMSCs do not display telomerase activity during differentiation along neural pathway. GDNF shows little impact on proliferation and telomerase activity of BMSCs.
Chen
Zhen-zhou
ZZ
Institute of Neuromedicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Xu
Ru-xiang
RX
Jiang
Xiao-dan
XD
Teng
Xiao-hua
XH
Li
Gui-tao
GT
Zhou
Yü-xi
YX
D000818:Animals; D001854:Bone Marrow Cells / Q000201:enzymology*; D002454:Cell Differentiation; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007150:Immunohistochemistry; D011817:Rabbits; D017154:Stromal Cells / Q000201:enzymology; D019098:Telomerase / Q000378:metabolism*
D051100:Glial Cell Line-Derived Neurotrophic Factor; D019098:Telomerase