Electrolyte redistribution in cystic fibrosis fibroblasts studied by electron probe X-ray microanalysis.
The elemental composition of fibroblasts from cystic fibrosis (CF) patients and from healthy controls was compared by means of electron probe X-ray micro-analysis. Significantly lower sodium levels (p < .01) and higher calcium levels (p < .02) were found in CF fibroblasts, indicating a disturbance of ion regulation in these cells. Treatment with Staphylococcus aureus protein A and gammaglobulin (proteins that may influence the ciliotoxic CF factor) did not change the elemental composition of the fibroblasts.
Roomans
G M
GM
Wenner-Gren Institute, University of Stockholm, Norrtullsgatan 16, S-11345 Stockholm, Sweden.
Ceder
O
O
Kollberg
H
H
D000293:Adolescent; D000328:Adult; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000378:metabolism* / Q000473:pathology; D004573:Electrolytes / Q000032:analysis / Q000378:metabolism*; D004577:Electron Probe Microanalysis / Q000379:methods*; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D012964:Sodium / Q000032:analysis / Q000378:metabolism
D004573:Electrolytes; D012964:Sodium; D002118:Calcium