title: Effect of postruminal glutamine supplementation on immune response and milk production in dairy cows.
abstract: Seventeen multiparous Holstein cows were used to examine the effect of an increased duodenal supply of Gln on immune function and production. Cows received continuous abomasal infusions of water (control: n = 8) or 300 g/d of Gln (n = 9) for 21 d starting within 48 h of calving. There were nonsignificant increases in milk and milk protein yields in response to Gln supplementation. Glutamine treatment had no effect on plasma glucose, nonesterified fatty acids (NEFA), or beta-hydroxybutyrate (BHBA) concentrations but did tend to increase plasma urea N concentration. The Gln treatment resulted in an increase of 108 microM in the plasma Gln concentration. Total essential AA concentrations decreased with the Gln treatment, whereas total nonessential AA concentrations were unaffected. T Lymphocyte proliferation did not differ between the control and Gln-treated cows. Treatment had no effect on the relative abundance of CD8 T cells but did increase the abundance of CD4 T cells. Cytokine production, as measured by IFN-gamma concentration determined in vitro in concanavalin-A-stimulated peripheral blood mononuclear cells, was similar between the treatments. Over the first 3 wk following calving, Gln supplementation had limited effects on milk production, metabolic parameters, and immune function.
authors.affiliation: Département des Sciences Animales, Université Laval, Ste-Foy, Quebec, Canada, G1K 7P4.
authors.forename: L
authors.initials: L
authors.lastname: Doepel
authors.forename: M
authors.initials: M
authors.lastname: Lessard
authors.forename: N
authors.initials: N
authors.lastname: Gagnon
authors.forename: G E
authors.initials: GE
authors.lastname: Lobley
authors.forename: J F
authors.initials: JF
authors.lastname: Bernier
authors.forename: P
authors.initials: P
authors.lastname: Dubreuil
authors.forename: H
authors.initials: H
authors.lastname: Lapierre
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000018:Abomasum / Q000187:drug effects; D000596:Amino Acids / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D016516:CD4-CD8 Ratio; D002417:Cattle / Q000276:immunology / Q000502:physiology*; D003208:Concanavalin A / Q000494:pharmacology; D004032:Diet; D019587:Dietary Supplements; D004435:Eating; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005973:Glutamine / Q000008:administration & dosage* / Q000097:blood; D007109:Immunity / Q000187:drug effects*; D007371:Interferon-gamma / Q000097:blood; D007774:Lactation / Q000187:drug effects*; D019344:Lactic Acid; D007958:Leukocyte Count; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D009584:Nitrogen / Q000032:analysis; D011247:Pregnancy; D013601:T-Lymphocytes
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D005973:Glutamine; D003208:Concanavalin A; D019344:Lactic Acid; D007371:Interferon-gamma; D009584:Nitrogen; D020155:3-Hydroxybutyric Acid