title: BDNF-mediated neurotransmission relies upon a myosin VI motor complex.
abstract: Brain-derived neurotrophic factor (BDNF) has been implicated in higher-order cognitive functions and in psychiatric disorders such as depression and schizophrenia. BDNF modulates synaptic transmission and plasticity primarily through the TrkB receptor, but the molecules involved in BDNF-mediated synaptic modulation are largely unknown. Myosin VI (Myo6) is a minus end-directed actin-based motor found in neurons that express Trk receptors. Here we report that Myo6 and a Myo6-binding protein, GIPC1, form a complex that can engage TrkB. Myo6 and GIPC1 were necessary for BDNF-TrkB-mediated facilitation of long-term potentiation in postnatal day 12-13 (P12-13) hippocampus. Moreover, BDNF-mediated enhancement of glutamate release from presynaptic terminals depended not only upon TrkB but also upon Myo6 and GIPC1. Similar defects in basal synaptic transmission as well as presynaptic properties were observed in Myo6 and GIPC1 mutant mice. Together, these results define an important role for the Myo6-GIPC1 motor complex in presynaptic function and in BDNF-TrkB-mediated synaptic plasticity.
authors.affiliation: Molecular Neurobiology Program, Skirball Institute of Biomolecular Medicine, Departments of Cell Biology, Physiology and Neuroscience, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yano
authors.forename: Ipe
authors.initials: I
authors.lastname: Ninan
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Teresa A
authors.initials: TA
authors.lastname: Milner
authors.forename: Ottavio
authors.initials: O
authors.lastname: Arancio
authors.forename: Moses V
authors.initials: MV
authors.lastname: Chao
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D046912:Multiprotein Complexes; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D020813:Receptor, trkB / Q000378:metabolism; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D019208:Brain-Derived Neurotrophic Factor; D002352:Carrier Proteins; C115271:Gipc1 protein, mouse; C492008:Gipc1 protein, rat; D046912:Multiprotein Complexes; D009479:Neuropeptides; C089785:myosin VI; D020813:Receptor, trkB; D018995:Myosin Heavy Chains