Dendritic cell recruitment following xenografting of pig fetal mesencephalic cells into the rat brain.
Following transplantation into the rat brain, porcine neuroblasts differentiate and integrate host tissue, but due to their xenogeneic nature, these cells are generally rejected within several weeks. This rejection is accompanied by infiltration of the graft by macrophages and alphabetaT lymphocytes, but so far nothing is known about the potential role of dendritic cells (DCs) in this process. DCs are professional antigen presenting cells that have the unique ability to prime naive T cells, thereby initiating an antigen-directed immune response. Here, we provide evidence for DC recruitment following the transplantation of pig mesencephalic neural cells into the striatum of LEW.1A rats, as indicated by the high number of OX62+ cells in the rejecting graft and the absence of V65 staining. DCs were found as early as 3 and 8 days postimplantation together with ED1+ and OX42+ cells. This early recruitment, which is probably due to the surgical procedure, might be a critical step in the rejection process, enabling DCs to be loaded with xenoantigens. The number of intracerebral DCs subsequently decreased, being barely detectable in older non-infiltrated xenografts. However, DCs re-appeared as they were observed in grafts infiltrated by macrophages and T cells, a phenomenon that usually precedes graft rejection. Interestingly, we observed a tight correlation between the number of DCs and that of R7.3+ T cells infiltrating the graft. In addition, DCs were often found in close proximity to alphabetaT cells and most expressed MHCII. Taken together, these findings give credence to a role for infiltrating DCs in the mediation of T cell responses to intracerebral xenografting.
Michel
Delphine C
DC
INSERM, U643, Nantes F44000, France.
Nerrière-Daguin
Véronique
V
Josien
Régis
R
Brachet
Philippe
P
Naveilhan
Philippe
P
Neveu
Isabelle
I
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001921:Brain / Q000166:cytology*; D002452:Cell Count / Q000379:methods; D003713:Dendritic Cells / Q000502:physiology*; D053331:Ectodysplasins / Q000378:metabolism; D004622:Embryo, Mammalian; D016332:Fetal Tissue Transplantation; D006084:Graft Rejection; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology; D009474:Neurons / Q000637:transplantation; D051381:Rats; D011917:Rats, Inbred Lew; D013552:Swine; D013997:Time Factors; D014183:Transplantation, Heterologous
D000943:Antigens, Differentiation; D053331:Ectodysplasins; C459465:OX-62 antigen, rat