[Antenatal determination of fetal RhD-blood type based on fetal DNA in plasma from the RhD-negative mother--secondary publication].
We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD-women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th-36th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.
Clausen
Frederik Banch
FB
H:S Rigshospitalet, H:S Blodbank, KI 2034, DK-2100 København Ø. frederik.banch.clausen@rh.dk
Krog
Grethe Risum
GR
Rieneck
Klaus
K
Nielsen
Leif Kofoed
LK
Lundquist
Rasmus
R
Finning
Kirsten
K
Dickmeiss
Ebbe
E
Hedegaard
Morten
M
Dziegiel
Morten Hanefeld
MH
D004247:DNA / Q000097:blood*; D015342:DNA Probes; D005091:Exons; D005260:Female; D005315:Fetal Diseases / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D012680:Sensitivity and Specificity
D015342:DNA Probes; D012204:Rh-Hr Blood-Group System; D004247:DNA