Protoplast fusion between Geotrichum candidium and Phanerochaete chrysosporium to produce fusants for corn stover fermentation.
Lignin impedes the digestion of corn stover when used as an animal feed. Phanerochaete chrysosporium is an efficient lignindegrader. Geotrichum candidum can be used to produce single-cell protein. In this study, protoplasts of the two fungi were prepared and fused. After screening, one of the fusants, Fusant R1, was selected for corn stover fermentation. It decreased lignin from 109 to 54 g/kg and increased protein from 48 to 67 g/kg in corn stover. Comparison with their parental strains indicated that the fusant obtained the lignin-degrading ability from P. chrysosporium and the protein-accumulating ability from G. candidium.
Zhang
Yong
Y
School of Animal Science and Veterinary, Shenyang Agricultural University, Shenyang, China 110161. syndzhy@yahoo.com.cn
Lin
Shu-Mei
SM
Zhu
Yu-Jing
YJ
Liu
Chang-Jiang
CJ
Dong
Yan
Y
Li
Fang-Fang
FF
Wu
Gao-Feng
GF
Wang
Hai-Yan
HY
Zhang
Jing-Hai
JH
D018533:Biomass; D002459:Cell Fusion; D005285:Fermentation; D005848:Geotrichum / Q000378:metabolism*; D007527:Isoenzymes / Q000032:analysis; D008031:Lignin / Q000378:metabolism; D020075:Phanerochaete / Q000378:metabolism*; D011523:Protoplasts / Q000166:cytology*; D003313:Zea mays / Q000378:metabolism*
D007527:Isoenzymes; D008031:Lignin