Comparison between immunofluorescence and PCR in detecting human papilloma virus in condyloma acuminata.
OBJECTIVE
To compare the effectiveness of immunofluorescence and polymerase chain reaction (PCR) in detecting human papilloma virus (HPV) in condyloma acuminata (CA).


METHODS
HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism (RFLP).


RESULTS
The positive detective rates of immunofluorescence and PCR were 56.67% (34/60) and 96.67% (58/ 60), respectively (P < 0.01). RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus, which accounted for 98.28%.


CONCLUSIONS
The sensibility of PCR is superior to that of immunofluorescence.
Liu
Fang
F
Department of Dermatology, Beijing Chaoyang Hospital, Beijing 100020. roseliufang@sohu.com
Wang
Jia-Bi
JB
Liu
Yue-Hua
YH
Zuo
Ya-Gang
YG
Man
Xiao-Hong
XH
D003218:Condylomata Acuminata / Q000821:virology*; D005260:Female; D052140:Human papillomavirus 11 / Q000235:genetics / Q000302:isolation & purification; D052163:Human papillomavirus 6 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction