Calorimetry of enzyme-catalyzed reactions.
This mini-review shows the valuable contributions of Professor Julian Sturtevant to the current applications of calorimetry to the study of enzyme-catalyzed reactions. The more recent applications of calorimetric techniques such as isothermal titration calorimetry and flow calorimetry to the study of enzyme kinetics, as well as the advantages on using calorimetric techniques in the determination of kinetic parameters of enzymes, is also discussed here.
Bianconi
M Lucia
ML
Programa de Biologia Estrutural, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Prédio do CCS, Bloco E, sala 27B, Ilha do Fundão, Rio de Janeiro, RJ 21941-590, Brazil. bianconi@bioqmed.ufrj.br
D002151:Calorimetry / Q000379:methods*; D002384:Catalysis; D004798:Enzymes / Q000737:chemistry*; D007700:Kinetics; D013816:Thermodynamics
D004798:Enzymes