Amplification of DNA encoding entire type I polyketide synthase domains and linkers from streptomyces species.
Polyketides are a group of bioactive compounds from bacteria, plants, and fungi. To increase the availability of analogs for testing, the active sites of polyketide synthases are often substituted with homologous domains having altered substrate specificities. This study reports the design of polymerase chain reaction primers that enables isolation of entire active site domains from type I polyketide synthases with native interdomain linkers. This bypasses the need for further genetic screening to obtain functional units for use in genetic engineering. This is especially important in bioprospecting projects exploring new environments for bioresources.
Chuck
Jo-Anne
JA
School of Natural Sciences, University of Western Sydney, Parramatta Campus, Locked Bag 1797, Penrith South, DC, 1797 NSW, Australia. j.chuck@uws.edu.au
Dunn
Catherine
C
Facultad
Fe E C D
FE
Nakazono
Chojin
C
Nikodinovic
Jasmina
J
Barrow
Kevin D
KD
D000935:Antifungal Agents / Q000494:pharmacology; D001234:Aspergillus niger / Q000187:drug effects; D001665:Binding Sites / Q000235:genetics; D017931:DNA Primers; D016680:Genome, Bacterial; D048630:Polyketide Synthases / Q000737:chemistry* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D017434:Protein Structure, Tertiary; D012988:Soil Microbiology; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D000935:Antifungal Agents; D017931:DNA Primers; D048630:Polyketide Synthases