Lactobacillus apodemi sp. nov., a tannase-producing species isolated from wild mouse faeces.
A Gram-positive, rod-shaped, non-endospore-forming bacterium, strain ASB1(T), able to degrade tannin, was isolated from faeces of the Japanese large wood mouse, Apodemus speciosus. Comparative analysis of the 16S rRNA gene sequence revealed that the strain could be assigned as a member of the genus Lactobacillus. The nearest phylogenetic neighbours were determined as Lactobacillus animalis DSM 20602(T) (98.9 % 16S rRNA gene sequence similarity) and Lactobacillus murinus ASF 361 (98.9 %). Subsequent polyphasic analysis, including automated ribotyping and DNA-DNA hybridization experiments, confirmed that the isolate represents a novel species, for which the name Lactobacillus apodemi sp. nov. is proposed. The DNA G+C content of the novel strain is 38.5 mol%. The cell-wall peptidoglycan is of type A4alpha L-lys-D-asp. The type strain is ASB1(T) (=DSM 16634(T)=CIP 108913(T)).
Osawa
Ro
R
Department of Bioresources and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe, 657-8501, Japan.
Fujisawa
Tomohiko
T
Department of Food Science and Technology, Nippon Veterinary and Animal Science University, Kyonan-cho 1-7-1, Musashino-shi, Tokyo, 180-8602, Japan.
Pukall
Rüdiger
R
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
D000818:Animals; D001424:Bacterial Infections / Q000382:microbiology* / Q000662:veterinary*; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D002473:Cell Wall / Q000737:chemistry; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D007778:Lactobacillus / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D051189:Murinae / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002265:Carboxylic Ester Hydrolases; C022711:tannase