[Regulation of PTHrP in proliferation and differentiation of chondrocytes of condyle in fetal mouse].
OBJECTIVE
To investigate the regulatory mechanism of parathyroid hormone-related protein (PTHrP) in proliferation and differentiation of chondrocytes of condyle in fetal mouse.


METHODS
Chondrocytes of condyle in fetal mouse were separated and cultured in vitro, the influence of PTHrP on proliferation and differentiation was observed.


RESULTS
After two weeks' culture in 0.01 nmol/L, 0.1 nmol/L, 1 nmol/L, 10 nmol/L human PTHrP, there was significant difference in the number of cartilage nodule formed between experiment group and control group (P<0.05), and there was no significant difference in 0.01 nmol/L group (P>0.05). Alkaline phosphatase (ALP) activity was significantly intensified in experiment group and control group (P<0.05). Meanwhile, it was found that this function of promotion was lessened after anti-PTHR antibody used.


CONCLUSIONS
It can be seen that PTHrP, via its receptor, can promote proliferation and differentiation of chondrocytes of condyle, which resemble its modulation mechanism in epiphyseal growth plate cartilage intramembrane in mandibule.
Liu
Lai-Kui
LK
Dept. of Oral Anatomy and Physiology, College of Stomatology, Nanjing Medical Science University, Nanjing 210029, China.
Jiang
Hong-Bing
HB
Yin
Xin-Min
XM
Li
Xiao-Qing
XQ
Yi
Xin-Zhu
XZ
D000818:Animals; D002454:Cell Differentiation; D019902:Chondrocytes; D006132:Growth Plate; D006801:Humans; D051379:Mice; D044162:Parathyroid Hormone-Related Protein
D044162:Parathyroid Hormone-Related Protein