Oscillatory reaction of catalase wrapped by liposome.
To date we have been studying the enzymatic oscillatory reaction caused by gradual entry of substrate via semi-permeable membrane. It has been found that many enzymes cause oscillatory reaction. Here we present an oscillatory reaction of enzyme wrapped by liposome. We used catalase as an enzyme, since its oscillatory reaction has been already investigated in detail in the absence of liposome. Distinct oscillation with shorter period than without liposome was obtained. On the other hand, it was shown that the presence of liposome facilitated the permeation rate of hydrogen peroxide through semi-permeable membrane. This is thought to be factor of shortening the oscillation period compared with that in the absence of liposome. Oscillation was also temperature dependent. Our finding may provide an important insight into the study of enzyme reaction taking part in rhythms in living systems.
Hideshima
Taketoshi
T
Department Chemistry, Faculty of Science, Chiba University, 1-33 Yayoicho, Inage-ku, Chiba 263-8522, Japan. hideshima@faculty.chiba-u.jp
Kato
Yoshiteru
Y
D000495:Allosteric Site; D002374:Catalase / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009991:Oscillometry; D013997:Time Factors
D008081:Liposomes; D002374:Catalase