Isolation of bacteriocinogenic Lactobacillus plantarum strains from ben saalga, a traditional fermented gruel from Burkina Faso.
A collection of lactic acid bacteria isolated from ben saalga, a traditional fermented gruel from Burkina Faso, was screened for bacteriocin production. Seven isolates were selected for their broad antimicrobial spectra, which overall included strains of Bacillus cereus, Bacillus licheniformis, Enterococcus faecalis, Listeria innocua, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli and Salmonella enterica. Cluster analysis of RAPD-PCR patterns revealed that six of the isolates represent different strains. The six selected strains were identified as Lactobacillus plantarum by 16S rDNA sequencing, species-specific PCR and multiplex PCR of the recA gene. PCR amplification revealed the presence of genes of the plantaricin cluster described in L. plantarum C11. Among them, strain 5.2.2 carried the largest number of genes from this cluster.
Omar
Nabil Ben
NB
Area de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, Campus Las Lagunillas s/n, 23071-Jaén, Spain.
Abriouel
Hikmate
H
Lucas
Rosario
R
Martínez-Cañamero
Magdalena
M
Guyot
Jean-Pierre
JP
Gálvez
Antonio
A
D001430:Bacteriocins / Q000096:biosynthesis*; D002050:Burkina Faso; D016000:Cluster Analysis; D003257:Consumer Product Safety; D004269:DNA, Bacterial / Q000032:analysis*; D005285:Fermentation; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D005784:Gene Amplification; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000302:isolation & purification* / Q000378:metabolism; D031782:Pennisetum / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D001430:Bacteriocins; D004269:DNA, Bacterial