Modulation of rat oligodendrocyte precursor cells by the chemokine CXCL12.
Migration, proliferation, and differentiation of oligodendrocyte precursor cells are essential for the assembly of myelin in the central nervous system. Knowledge on the regulation of these precursor cells is therefore of great importance for the understanding of developmental myelination and remyelination in demyelinating diseases. Here, we show that primary rat oligodendrocyte precursor cells express the chemokine receptor CXCR4. Stimulation with the ligand CXCL12 (SDF-1 alpha) leads to intracellular Ca elevation. Furthermore, 10 ng/ml CXCL12 augmented differentiation of precursors into mature oligodendrocytes. Migration toward growth factor conditioned medium was inhibited by CXCL12, while proliferation was only slightly modulated. The effect of CXCL12 on both migration and differentiation was blocked using a G protein antagonist. These data suggest a role for CXCL12 and oligodendroglial CXCR4 receptors during developmental myelination and repair in demyelinating diseases of the central nervous system.
Maysami
Samaneh
S
Department of Neurology, Medical School Hannover, Hannover, Germany.
Nguyen
Dan
D
Zobel
Franziska
F
Pitz
Caroline
C
Heine
Sandra
S
Höpfner
Michael
M
Stangel
Martin
M
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002118:Calcium / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000494:pharmacology*; D017931:DNA Primers; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D009836:Oligodendroglia / Q000187:drug effects / Q000276:immunology*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D019718:Receptors, CXCR4 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054377:Chemokine CXCL12; D019743:Chemokines, CXC; C486264:Cxcr4 protein, rat; D017931:DNA Primers; D005904:Glial Fibrillary Acidic Protein; D019718:Receptors, CXCR4; D002118:Calcium