[Effect of platelet derived growth factor BB on healing of medial collateral ligament in rats].
OBJECTIVE
To examine an effect of the locally-used platelet derived growth factor-BB (PDGF-BB) on the healing of the medial collateral ligament (MCL) in the knee joints of rats.


METHODS
Forty-eight rats were equally randomly divided into 2 groups: the experimental group (group A) and the control group (group B). MCL of all the rats were ruptured to establish the wound models. In group A, 5 microg of PDGF-BB was locally injected in the wound of each rat and then the wound was sutured; but in group B, the wound was only sutured. After 2 weeks, histological evaluations were performed to determine whether PDGF-BB could promote the healing of MCL.


RESULTS
There were significantly more fibroblasts formed during the ligament healing process in group A than in group B (213.44 +/- 15.32 vs. 180.42 +/- 12.78, P < 0.01). The fibroblasts were more mature and more regularly-arranged in group A than in group B. The type, content, and crosslink of the collagen were improved to a greater extent in group A than in group B (P < 0.01).


CONCLUSIONS
PDGF can promote the healing of the injured ligament.
Ren
Yizhong
Y
Department of Orthopedic Surgery, Second Affiliated Hospital of Inner Mongolia Medical College, Inner Mongolia Medical College, Hohhot Neimenggu, 010030, PR China. Pocesdonone@hotmail.com
Li
Wenqi
W
Liu
Xiaomin
X
D000818:Animals; D000077214:Becaplermin; D004195:Disease Models, Animal; D008297:Male; D017888:Medial Collateral Ligament, Knee / Q000187:drug effects* / Q000293:injuries; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin