Importance of the heparin-binding domain of fibronectin for enhancing cell adhesion activity of the recombinant fibronectin.
We have previously shown that the recombinant human fibronectin (FN) fragment composed of central cell binding domains (CCBD) spanning the ninth and tenth type III domains promotes cell adhesion and proliferation of osteoblasts. In the present study, we investigated the biological potency of heparin-binding domain (HBD) of FN spanning the twelfth and fourteenth type III domains. The HBD of FN significantly enhances the RGD-containing CCBD-mediated cell adhesion and proliferation in HOS cells (P < 0.05).
Kim
Ji-Hyun
JH
Department of Biochemistry, Inha University College of Medicine, Jung-Gu, Incheon 400-712, Korea.
Park
Sun-Ok
SO
Jang
Hyun-Jun
HJ
Jang
Jun-Hyeog
JH
D001665:Binding Sites; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005353:Fibronectins / Q000737:chemistry* / Q000502:physiology; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D010006:Osteoblasts / Q000502:physiology; D010446:Peptide Fragments / Q000502:physiology*; D011994:Recombinant Proteins / Q000737:chemistry; D013329:Structure-Activity Relationship
D005353:Fibronectins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D006493:Heparin