Kinetic spectrophotometric analysis of pantoprazole in commercial dosage forms.
A kinetic spectrophotometric method has been developed which is based on the oxidation of pantoprazole with Fe(III) in sulfuric acid medium. Fe(III) subsequently reduces to Fe(II), which is coupled with potassium ferricyanide to form Prussian blue. The reaction is followed spectrophotometrically by measuring the increase in absorbance with time (1-8 min) at 725 nm. The initial rate method is adopted for constructing the calibration graph, which is linear in the concentration range of 5-90 microg ml(-1). The regression analysis yields the calibration equation, nu = 3.467 x 10(-6) + 4.356 x 10(-5)C. The limits of detection and quantitation are 1.46 and 4.43 microg ml(-1), respectively. The proposed method was optimized and validated both statistically and through recovery studies. The experimental true bias of all samples is < +/-2.0%. The method has been successfully applied to the determination of pantoprazole in pharmaceutical preparations.
Rahman
Nafisur
N
Department of Chemistry, Aligarh Muslim University, Aligarh 202002, Uttar Predesh, India. cht17nr@yahoo.co.in
Bano
Zehra
Z
Azmi
Syed Najmul Hejaz
SN
D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000032:analysis*; D005292:Ferricyanides / Q000737:chemistry*; D005295:Ferrocyanides / Q000138:chemical synthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry; D007700:Kinetics; D015394:Molecular Structure; D010084:Oxidation-Reduction; D000077402:Pantoprazole; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013464:Sulfuric Acids / Q000737:chemistry; D013997:Time Factors
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D005292:Ferricyanides; D005295:Ferrocyanides; D013464:Sulfuric Acids; D000077402:Pantoprazole; D007501:Iron; C033158:sulfuric acid; C000170:ferric ferrocyanide; C028033:potassium ferricyanide