Poly(N,N-dimethylacrylamide)-grafted polyacrylamide: a self-coating copolymer for sieving separation of native proteins by CE.
The potential of a series of newly synthesized poly(N,N-dimethylacrylamide) (PDMA) grafted polyacrylamide (PAM) copolymers (P(AM-PDMA)) as a replaceable separation medium for protein analysis was studied. A comparative study with and without copolymers was performed; the separation efficiency, analysis reproducibility and protein recovery proved that the P(AM-PDMA) copolymers were efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. Furthermore, the size-dependent retardation of native proteins in a representative P(AM-PDMA) copolymer was demonstrated by Ferguson analysis. The results showed that the P(AM-PDMA) copolymers combine the good coating property of PDMA and the sieving property of PAM and could be applied as a sieving matrix for the analysis of native proteins.
Zhang
Jiyou
J
Groupe de Chimie Analytique de Paris Sud, Faculté de Pharmacie, 5 Rue JB Clement, F-92290 Chatenay-Malabry, France.
Tran
Nguyet Thuy
NT
Weber
Jérémie
J
Slim
Cyrine
C
Viovy
Jean-Louis
JL
Taverna
Myriam
M
D000178:Acrylamides / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*
D000178:Acrylamides; D011108:Polymers; D011506:Proteins; C429790:poly(N,N-dimethylacrylamide)