[Effect of xinnao shutong capsule on cardiac muscle cell apoptosis and protein expressions of Bcl-2 and Bax in hyperlipidemia rats after myocardial infarction].
OBJECTIVE
To investigate the effect of Xinnao Shutong Capsule, whose main ingredients gross saponins from Tribulus Terrestris L (GSTT) on cardiac muscle cell (CMC) apoptosis and expressions of Bcl-2 Compound rat model of and Bax in murine model of hyperlipemia after myocardial infarction (MI).


METHODS
MI and hyperlipemia was adopted. TUNEL assay was applied to detect CMC apoptosis after 4 weeks' administration of GSTT or simvastatin, and immunohistochemical SP technique was used to detect the expressions of Bcl-2 and Bax protein.


RESULTS
GSTT can relieve the damage of CMC and attenuate the ventricular remodeling after MI; high dose of GSTT and simvastatin could decrease CMC apoptosis (P<0.05), and lower Bax protein expression (P < 0.05); and there was no significant difference among the effects in all the treated group (P> 0.05).


CONCLUSIONS
GSTT can reduce CMC apoptosis through regulating protein expressions of Bcl-2 and Bax, which may be one of the mechanisms of its anti-ventricular-remodeling effects after MI.
Guo
Yan
Y
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing.
Yin
Hui-jun
HJ
Shi
Da-zhuo
DZ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002214:Capsules; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D006949:Hyperlipidemias / Q000150:complications / Q000188:drug therapy*; D008297:Male; D009203:Myocardial Infarction / Q000150:complications / Q000188:drug therapy*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology*; D008517:Phytotherapy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics
D002214:Capsules; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein