[Study on interaction of adriamycin and DNA with a resonance light scattering technique and its DNA analytical application].
The interaction of adriamycin (ADM) and DNA results in strongly enhanced resonance light scattering (RLS) spectra characterized by two peaks at 322 and 564 nm, respectively. The interaction of ADM and DNA was investigated by using absorption spectra, fluorescence spectra, and RLS spectra. The results indicate that RLS spectra have the highest sensitivity. It was found that the enhanced RLS intensity at 322 nm was proportional to the concentration of DNA in the range of 0-8.0 microg x mL(-1). An RLS method for the determination of DNA was accordingly described. The limit of determination was 36.8 and 40.1 ng x mL(-1) for calf thymus DNA and fish sperm DNA, respectively. The proposed method has a high sensitivity and good selectivity due to the specificity of the interaction of ADM and DNA.
Feng
Shuo
S
College of Chemistry and Environment Science, Hebei University, Baoding, China.
Li
Zheng-ping
ZP
Zhang
Shu-hong
SH
Fang
Zheng
Z
D000818:Animals; D004247:DNA / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry*; D005399:Fishes; D008297:Male; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013094:Spermatozoa / Q000737:chemistry
D004317:Doxorubicin; D004247:DNA; C427203:calf thymus DNA