[AFLP analysis on genetic diversity of wild Dactylis glomerata L. germplasm resources].
Thirty-seven accessions of wild Dactylis glomerata L. were used in assessment of genetic diversity by means of amplified fragment length polymorphism (AFLP). Nine primer pairs were selected from 64 preparations and then used to analyze the plant materials. A total of 400 DNA fragments were amplified, among which 336 (84.0%) were polymorphic. Genetic distance, from 0.0692-0.4214 among accessions, was calculated by the NTSYS-pc software. A dendrogram was constructed using UPGMA based on genetic distance matrix. Eight clusters were defined at the genetic similarity of 0.81. A two-dimensional PCO plot representing relationships among accessions was also produced by EIGEN, demonstrating the same distribution pattern as from the cluster analysis. Based on molecular analysis, the genetic variation of D. glomerata germplasms was closely associated with ploidy levels and geographical distributions.
Peng
Yan
Y
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China. pengyanlee@163.com
Zhang
Xin-Quan
XQ
Liu
Jin-Ping
JP
Yi
Yang-Jie
YJ
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D031745:Dactylis / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D010802:Phylogeny
D017931:DNA Primers