title: Selective sample preparation with bioaffinity columns prepared by the sol-gel method.
abstract: Bioaffinity materials prepared by entrapping highly selective bioligands in the pores of a sol-gel glass offer unique advantages for the clean-up of complex sample matrices. The inclusion of sol-gel bioaffinity columns frequently allows the efficient elimination of interfering matrix components and enrichment of analytes in a simple step. After introducing the basic principles the paper reviews the methods for the production of protein-doped sol-gel materials and discusses the characteristics of sol-gel affinity columns by comparing their stability, selectivity, binding capacity and reusability. The potential of sample clean-up with sol-gel affinity columns is demonstrated giving applications in environmental, food and clinical analysis.
authors.affiliation: Department of Analytical and Food Chemistry, University of Vienna, Währinger Strasse 38, A-1090 Vienna, Austria. margit.cichna@univie.ac.at
authors.forename: Margit
authors.initials: M
authors.lastname: Cichna-Markl
mesh_terms: D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D005782:Gels; D012680:Sensitivity and Specificity
chemical_list: D005782:Gels