A monoclonal antibody to hippuric acid: an improved enzyme-linked immunosorbent assay for biological monitoring of toluene exposure.
A novel monoclonal antibody (MAb) to hippuric acid (HA) was prepared using an HA analog, N-alpha-benzoyl-lysine, as an immunogen. An enzyme-linked immunosorbent assay (ELISA) for HA was established using the anti-HA MAb named HA01BL. When the specificity of the MAb was analyzed by the ELISA system, the MAb was revealed to be less reactive to methylhippuric acids and to be more specific to HA than previously reported polyclonal antibodies. The detection limit of HA by the ELISA was approximately 1 microg/mL. The urinary HA concentration determined by the ELISA system correlated well with that obtained by high performance liquid chromatography.
Inagaki
Hirofumi
H
Department of Hygiene and Public Health, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan. hrfmi@nms.ac.jp
Minami
Masayasu
M
Hirata
Kimiko
K
Kawada
Tomoyuki
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004781:Environmental Exposure / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006626:Hippurates / Q000276:immunology* / Q000652:urine; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000276:immunology; D051379:Mice; D014050:Toluene / Q000506:poisoning*
D000911:Antibodies, Monoclonal; D006626:Hippurates; C512406:N-alpha-benzoyllysine; D014050:Toluene; D008239:Lysine; C030514:hippuric acid