Reclassification of the sulfate- and nitrate-reducing bacterium Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov.
Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925(T)) was found to use nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925(T) was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other nitrate- and sulfate-reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925(T). This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 degrees C (range 16-50 degrees C) and the optimum NaCl concentration for growth was 0.1 % (range 0-5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2(T) (=DSM 1925(T)=NCIMB 9442(T)=ATCC 33405(T)).
López-Cortés
Alejandro
A
Laboratorio de Ecología Microbiana Molecular, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Mar Bermejo 195, Playa Palo Santa Rita, La Paz, Baja California Sur, 23090, Mexico.
Fardeau
Marie-Laure
ML
Laboratoire de Microbiologie IRD, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, ESIL-GBMA, Case 925, 13288 Marseille Cedex 9, France.
Fauque
Guy
G
Laboratoire de Microbiologie, Géochimie et Ecologie Marines, UMR CNRS 6117, Campus de Luminy, Case 901, 13288 Marseille Cedex 9, France.|Laboratoire de Microbiologie IRD, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, ESIL-GBMA, Case 925, 13288 Marseille Cedex 9, France.
Joulian
Catherine
C
BRGM, Environment and Process Division, Biotechnology Unit, 2, avenue Claude Guillemin, 45060 Orléans Cedex 2, France.
Ollivier
Bernard
B
Laboratoire de Microbiologie IRD, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, ESIL-GBMA, Case 925, 13288 Marseille Cedex 9, France.
D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003901:Desulfovibrio / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D016969:Desulfovibrio vulgaris / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D020459:Genes, rRNA / Q000235:genetics; D006131:Growth Inhibitors / Q000494:pharmacology; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction; D010461:Peptones / Q000378:metabolism; D010802:Phylogeny; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006131:Growth Inhibitors; D009566:Nitrates; D010461:Peptones; D000644:Quaternary Ammonium Compounds; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D012965:Sodium Chloride