Boronic acids as ligands for affinity chromatography.
A review on the principles and applications of boronic acids as affinity ligands for the chromatographic separation of carbohydrates, nucleic acid components, glycoproteins, and other small biomolecules. The mechanisms of interactions between boronate ligands and analytes are described. Various boronate ligands and supports are discussed. Examples of the use of boronate affinity chromatography for separation of each class of analytes are presented.
Liu
Xiao-Chuan
XC
Department of Chemistry, California State Polytechnic University, Pomona, CA 91768, USA. xcliu@csupomona.edu
D001897:Boronic Acids / Q000008:administration & dosage / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D006023:Glycoproteins / Q000008:administration & dosage / Q000737:chemistry*; D008024:Ligands; D011485:Protein Binding / Q000502:physiology
D001897:Boronic Acids; D006023:Glycoproteins; D008024:Ligands