Production of protective antibodies against Porphyromonas gingivalis strains by immunization with recombinant gingipain domains.
We studied the effect of antibodies against Porphyromonas gingivalis gingipain domains, preparing them against three recombinant fragments of RgpA (catalytic domain, r-Rgp CAT; hemagglutinin domains, r-Rgp 44 and r-Rgps 15-27) and one fragment of Kgp (catalytic domain, r-Kgp CAT). Enhancement of opsonization and killing by human polymorphonuclear leukocytes were measured in the noninvasive FDC 381 and invasive W50 strains of P. gingivalis. Anti-r-Rgp 44 was the most effective in both strains of P. gingivalis. The present findings lead us to recommend RgpA 44 as a candidate immunogen for vaccines against P. gingivalis.
Yasaki-Inagaki
Yuriko
Y
Department of Periodontics, Tokyo Dental College, Chiba, Japan.
Inagaki
Satoru
S
Yamada
Satoru
S
Okuda
Katsuji
K
Ishihara
Kazuyuki
K
D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000276:immunology* / Q000378:metabolism; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D001770:Blood Bactericidal Activity / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins / Q000276:immunology; D049449:Luminescence; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000276:immunology*; D017434:Protein Structure, Tertiary; D014614:Vaccines, Synthetic / Q000276:immunology
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D009895:Opsonin Proteins; D014614:Vaccines, Synthetic; D003546:Cysteine Endopeptidases