title: In vivo transduction of an Stx-encoding phage in ruminants.
abstract: We assessed the ability of a kanamycin-marked Stx phage to move into a commensal, ovine Escherichia coli strain in the ruminant gastrointestinal tract. Transduction was detected in 19/24 sheep tested, resulting in the recovery of 47 transductants. Subtherapeutic doses of the quinolone antibiotic enrofloxacin did not increase the rate of transduction.
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50010, USA. ncornick@iastate.edu
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Cornick
authors.forename: Amy F
authors.initials: AF
authors.lastname: Helgerson
authors.forename: Volker
authors.initials: V
authors.lastname: Mai
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Ritchie
authors.forename: David W K
authors.initials: DW
authors.lastname: Acheson
mesh_terms: D000818:Animals; D003090:Coliphages / Q000235:genetics* / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D019453:Escherichia coli O157 / Q000235:genetics / Q000821:virology; D041981:Gastrointestinal Tract / Q000382:microbiology; D012418:Ruminants / Q000382:microbiology; D012756:Sheep / Q000382:microbiology*; D022621:Shiga Toxin / Q000235:genetics*; D014161:Transduction, Genetic
chemical_list: D022621:Shiga Toxin