title: Gene amplification using DNA from human spot urine samples.
abstract: The purpose of this study was to test the amplification of DNA from human urinary sediment for molecular epidemiological studies. Twenty-six urine samples were obtained from healthy volunteers. Polymerase chain reactions (PCR) for methylenetetrahydrofolate reductase (MTHFR), beta-globin, and N-acetyltransferase 2 (NAT2) was conducted using genomic DNA isolated from the urine. The MTHFR and beta-globin genes were amplified successfully from all the urine DNA samples while the NAT2 gene was amplified in 88.5% of cases. The median yield of DNA was 0.28 microg from the 10 ml urine samples, sufficient amounts of DNA being contained in urinary sediments for amplification of all three genes. This result indicates that urine can be used as a DNA source for PCR-based molecular epidemiological studies.
authors.affiliation: Department of Preventive Medicine, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Cheong-Sik
authors.initials: CS
authors.lastname: Kim
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Daehee
authors.initials: D
authors.lastname: Kang
authors.forename: Yun-Chul
authors.initials: YC
authors.lastname: Hong
authors.forename: Keun-Young
authors.initials: KY
authors.lastname: Yoo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D002648:Child; D004247:DNA / Q000652:urine*; D005240:Feasibility Studies; D005260:Female; D005914:Globins / Q000235:genetics*; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D017720:Molecular Epidemiology / Q000379:methods*; D016133:Polymerase Chain Reaction; D012016:Reference Values
chemical_list: D005914:Globins; D004247:DNA; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human