Biotin-protein ratios and stability of biotinylated immunoglobulins as standards for the quantitation of biotin-binding immunoglobulins.
Biotin-binding IgG in human sera was quantitated using F(ab')(2)anti-human IgG-coated multiwell microplates (Muratsugu, M. et al. 2003, Biol. Pharm. Bull., 26, 1605-1608). The biotin-protein ratio of biotinylated IgG, which was used as standard in the assay, was very important to quantitate the level of biotin-binding IgG. We investigated a synthesis method of biotinylated human immunoglobulins, how to determine the biotin-protein ratio of the biotinylated proteins, and their stability to prepare standards for measuring biotin-binding IgG, IgA, and IgM.
Yazawa
Ayaka
A
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University, Japan.
Fukuoka
Kyoko
K
Honda
Hidemi
H
Fukui
Toru
T
Muratsugu
Makoto
M
D001710:Biotin / Q000493:pharmacokinetics*; D019921:Biotinylation; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007136:Immunoglobulins / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D012710:Serum Albumin, Bovine; D001710:Biotin