title: Human plasma DNP level after severe brain injury.
abstract: OBJECTIVE
  To determine the relationship between DNP level after human severe brain injury and hyponatremia as well as isorrhea.
  
  
  METHODS
  The peripheral venous plasma as control was collected from 8 volunteers. The peripheral venous plasma from 14 severe brain injury patients were collected in the 1, 3, 7 days after injury. Radioimmunoassay was used to detect the DNP concentration. Meanwhile, daily plasma and urine electrolytes, osmotic pressure as well as 24 h liquid intake and output volume were detected.
  
  
  RESULTS
  The normal adult human plasma DNP level was 62.46 pg/ml+/-27.56 pg/ml. In the experimental group, the plasma DNP levels were higher from day 1 to day 3 in 8 of the 14 patients than those in the control group (P(1)=0.05, P(3)=0.03). Negative fluid balance occurred in 8 patients and hyponatremia in 7 patients. The increase of plasma DNP level was significantly correlated with the development of a negative fluid balance (r =-0.69, P<0.01) and hyponatremia (chi(2) =4.38, P<0.05).
  
  
  CONCLUSIONS
  The increase of plasma DNP level is accompanied by the enhancement of natriuretic and diuretic responses in severe brain-injured patients, which is associated with the development of a negative fluid balance and hyponatremia after brain injury.
authors.affiliation: Department of Nuclear Medicine, Affiliated Hospital of Nantong University, Nantong 226001, China. yilugao@hotmail.com
authors.forename: Yi-lu
authors.initials: YL
authors.lastname: Gao
authors.forename: Hui-ning
authors.initials: HN
authors.lastname: Xin
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Ji-wei
authors.initials: JW
authors.lastname: Fan
mesh_terms: D000328:Adult; D001930:Brain Injuries / Q000097:blood* / Q000150:complications; D004546:Elapid Venoms / Q000097:blood*; D005260:Female; D006801:Humans; D007010:Hyponatremia / Q000209:etiology; D036341:Intercellular Signaling Peptides and Proteins; D019586:Intracranial Hypertension / Q000097:blood / Q000209:etiology; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D014883:Water-Electrolyte Imbalance / Q000097:blood
chemical_list: C075597:Dendroaspis natriuretic peptide; D004546:Elapid Venoms; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011933:Reagent Kits, Diagnostic