A biased detection of the flexible genome content: missing large restriction fragments in comparative genomics.
Gene content variation between closely related bacteria is largely due to mobile elements and gene erosion. Various techniques are available to assess this variation, yet biased pools of restriction fragments have been used in several comparative genomics studies. Excluding important large restriction fragments from the actual hybridisation or from post-hybridisation amplification reactions compromise the comprehensiveness of these studies.
van Passel
M W J
MW
Department of Biochemistry and Biophysics, University of Arizona, Tucson, AZ 85721, USA. mvpassel@email.arizona.edu <mvpassel@email.arizona.edu>
D001419:Bacteria / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes