A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress.
Mung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. It was strongly suppressed by chilling stress. This implies that exogenous treatment with BR could allow the plant to recover from the inhibited growth caused by chilling. In this study, we used proteomics to investigate whether the mung bean epicotyl can be regulated by brassinosteroids under conditions of chilling stress. Mung bean epicotyls whose growth was initially suppressed by chilling partly recovered their ability to elongate after treatment with 24-epibrassinolde; 17 proteins down-regulated by this chilling were re-up-regulated. These up-regulated proteins are involved in methionine assimilation, ATP synthesis, cell wall construction and the stress response. This is consistent with the re-up-regulation of methionine synthase and S-adenosyl-L-methionine synthetase, since chilling-inhibited mung bean epicotyl elongation could be partially recovered by exogenous treatment with DL-methionine. This is the first proteome established for the mung bean species. The regulatory relationship between brassinosteroids and chilling conditions was investigated, and possible mechanisms are discussed herein.
Huang
Bin
B
Institute of Plant Biology, National Taiwan University, Taipei, Taiwan.
Chu
Chien-Hua
CH
Chen
Shu-Ling
SL
Juan
Hsueh-Fen
HF
Chen
Yih-Ming
YM
D000222:Adaptation, Physiological / Q000187:drug effects*; D060406:Brassinosteroids; D002777:Cholestanols / Q000494:pharmacology*; D003080:Cold Temperature; D018548:Cotyledon / Q000737:chemistry* / Q000187:drug effects; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry* / Q000187:drug effects* / Q000201:enzymology; D025461:Feedback, Physiological / Q000187:drug effects; D020869:Gene Expression Profiling; D008715:Methionine / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
D060406:Brassinosteroids; D002777:Cholestanols; D010940:Plant Proteins; D013260:Steroids, Heterocyclic; D003577:Cytochrome P-450 Enzyme System; D008715:Methionine; C023623:brassinolide