title: Early and late histopathological changes in renal allografts procured by laparoscopic donor nephrectomy.
abstract: BACKGROUND
  Subcapsular cortical damage (SCCD) is a characteristic pathological change, which is seen in renal allograft biopsy specimen obtained immediately after laparoscopic donor nephrectomy (LDN). In this study we evaluated the early and late histopathological effect of SCCD examining renal allograft biopsy specimens obtained after kidney transplantation.
  
  
  METHODS
  Laparoscopic donor nephrectomy was performed on 250 donors between July 2000 and September 2004 in our institution. Allograft biopsy of all 250 kidneys was performed immediately after LDN (zero hour biopsy). Of 250 biopsy specimens, 105 were diagnosed as SCCD and the allograft biopsy specimens obtained from 105 recipients after transplantation were enrolled in this study. All specimens were routinely examined by using light microscopy and immunofluorescence.
  
  
  RESULTS
  In 87 patients 256 allograft biopsy specimens did not show post-pathological changes of SCCD. Twenty biopsy specimens obtained from 18 recipients showed tubular necrosis and a partly tubular regeneration and patchy interstitial hemorrhage, which seemed to be caused by SCCD. Ten allograft biopsy specimens from nine recipients demonstrated residual pathologic changes in SCCD early after transplantation. The other 10 specimens from nine recipients had abnormal histopathologic changes including interstitial edema and fibrosis, tubular atrophy and glomerular sclerosis in the subcapsular cortex late after transplantation.
  
  
  CONCLUSIONS
  The early change of SCCD after transplantation remained intact and partial regeneration and the late showed subcapsular fibrosis with glomerular sclerosis. The incidence of the subcapsular lesions due to SCCD after transplantation was 17% in 105 allografts.
authors.affiliation: Department of Urology, Kidney Center, Tokyo Women's Medical University, Tokyo, Japan. houichi@aol.com
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Shimizu
authors.forename: Kazunari
authors.initials: K
authors.lastname: Tanabe
authors.forename: Naoshi
authors.initials: N
authors.lastname: Miyamoto
authors.forename: Tadahiko
authors.initials: T
authors.lastname: Tokumoto
authors.forename: Hideki
authors.initials: H
authors.lastname: Ishida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Toma
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamaguchi
mesh_terms: D001706:Biopsy; D005355:Fibrosis; D006801:Humans; D007668:Kidney / Q000473:pathology; D007673:Kidney Cortex Necrosis / Q000473:pathology*; D016030:Kidney Transplantation / Q000473:pathology*; D010535:Laparoscopy / Q000009:adverse effects*; D019520:Living Donors; D012598:Sclerosis; D020858:Tissue and Organ Harvesting / Q000009:adverse effects*; D014184:Transplantation, Homologous