Immunomagnetic beads assay for the detection of botulinum neurotoxin types C and D.
An immunomagnetic beads assay for the simultaneous quantification of botulinum neurotoxin types C and D was developed. Specific monoclonal antibodies against the heavy chain of the toxin and affinity-purified biotinylated polyclonal antibodies (pAbs) were used. The antibodies were preincubated with the sample. The complex being formed was then captured by magnetic beads coated with antimouse IgG. Streptavidin-poly-horseradish peroxidase, a signal amplifier, bound to the biotinylated pAb. A maximum sensitivity of approximately 0.3 minimal lethal doses for mice per milliliter was achieved with culture supernatants of both toxin types.
Gessler
Frank
F
Institute for Applied Biotechnology in the Tropics, University of Göttingen, 37077 Göttingen, Germany. fgessle@gwdg.de
Hampe
Katrin
K
Schmidt
Michaela
M
Böhnel
Helge
H
D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D001710:Biotin; D001905:Botulinum Toxins / Q000032:analysis*; D003014:Clostridium botulinum / Q000737:chemistry*; D007118:Immunoassay; D018189:Immunomagnetic Separation; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; C096325:botulinum toxin type D; D001710:Biotin; D001905:Botulinum Toxins; C096324:botulinum toxin type C