[Identification of the components and products of hydrolysis in acetylleucomycin by LC-MS].
OBJECTIVE
To identify the components of acetylleucomycin and its hydrolytic products by LC-MS.


METHODS
Acetylleucomycin was separated on a Diamonsil C18 column with 0.1 mol x L(-1) ammonium acetate-acetontrile (35 : 65) as mobile phase. The LC-MS was equipped with an electorspray ion source (ESI), which was set at the positive ion mode, and the mass spectra of each component in chromatogram were obtained with difference cone voltage.


RESULTS
The components of acetylleucomycin and its hydrolytic products can be separated by HPLC. The components were identified according to the molecular weight and its major mass fragment ions. The major components identified in domastic acetylleucomycin were acetylleucomycin A4, A5; acetylleucomycin A1, A3; acetylleucomycin A6, A7, and acetylleucomycin A13. The hydrolytic products of acetylleucomycin were not kitasamycin, but some non-complete hydrolytic product.


CONCLUSIONS
The method is rapid, sensitive and specific. It' s suitable to application in the fields of multi-components antibiotics analysis.
Hu
Min
M
National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
Hu
Chang-Qin
CQ
D002853:Chromatography, Liquid / Q000379:methods; D006868:Hydrolysis; D015570:Josamycin / Q000032:analysis / Q000737:chemistry; D015939:Kitasamycin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D007933:Leucomycins / Q000032:analysis / Q000737:chemistry; D018942:Macrolides / Q000032:analysis / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D007933:Leucomycins; D018942:Macrolides; D015939:Kitasamycin; C071430:leucomycin A6; C111299:leucomycin A5; C401285:leucomycin A7; D015570:Josamycin