Fluorophore-encapsulated solid-supported bilayer vesicles: a method for studying membrane permeation processes.
This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.
Williams
Thomas L
TL
Department of Chemistry, University of Bath, Bath BA2 7AY, United Kingdom.
Vareiro
Margarida M L M
MM
Jenkins
A Toby A
AT
D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods; D013499:Surface Properties; D013997:Time Factors
D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D010743:Phospholipids; C059778:7,7-dimethyl-5,8-eicosadienoic acid; D010741:Phospholipases A