[Effects of MTNR1A gene on litter size in a large white and a landrace herd].
Two pairs of primers were designed based on the known sequence in GenBank for amplification of MTNR1A gene in a Large White and a Landrace herd. Using PCR-SSCP (single strand conformation polymorphism), we found a single nucleotide polymorphism(SNP) within the product amplified from the first pair of primers. PCR products from randomly selected different genotypes were sequenced after were recovered and purified. Results revealed a synonymous single base mutation(G-->A) at +159bp(sequence numbering based on Genbank accession number U73326) for the BB genotype. When analyzed for association with litter size traits, this MTNR1A SNP was found to have no significant effect on litter size traits.
Wang
Xiao-Feng
XF
The Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100094, China. wxfeng2003@126.com
Wang
Ai-Guo
AG
Fu
Jin-Lian
JL
Li
Zong-Jin
ZJ
Han
Li-Ping
LP
Yang
Shu-Ming
SM
D000818:Animals; D001483:Base Sequence; D005260:Female; D005838:Genotype; D008098:Litter Size; D008297:Male; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D044094:Receptors, Melatonin / Q000235:genetics* / Q000378:metabolism; D034421:Sus scrofa / Q000235:genetics* / Q000502:physiology*
D044094:Receptors, Melatonin