title: The rggC locus, with a frameshift mutation, is involved in oxidative stress response by Streptococcus thermophilus.
abstract: In Streptococcus thermophilus, the locus rggC contains a frameshift mutation and thus consists of two open reading frames (ORFs), rggC (1) and rggC (2), which encode proteins exhibiting similarity with the Rgg transcriptional regulator family. In this work, mutants showing a partial deletion of rggC (1) and rggC (2 )were constructed and their response to menadione, a superoxide-generating compound, was analysed. These mutants exhibited different behaviour to this oxidative stress compared with the wild-type strain. Analysis of this locus among 21 strains of S. thermophilus showed a polythymine tract length variability and a strain-dependant adenine residue could be found upstream of this repeat. This interstrain polymorphism supports evidence for the hypothesis that the rggC locus is phase variable.
authors.affiliation: Laboratoire de Génétique et Microbiologie, UMR INRA 1128, IFR 110, Faculté des Sciences et Techniques de l'Université Henri Poincaré, Campus de Grignard, BP239, 54506 Vanoeuvre-lès-Nancy, France.
authors.forename: Annabelle
authors.initials: A
authors.lastname: Fernandez
authors.forename: Frédéric
authors.initials: F
authors.lastname: Borges
authors.forename: Brigitte
authors.initials: B
authors.lastname: Gintz
authors.forename: Bernard
authors.initials: B
authors.lastname: Decaris
authors.forename: Nathalie
authors.initials: N
authors.lastname: Leblond-Bourget
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D016368:Frameshift Mutation / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D016680:Genome, Bacterial / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D018384:Oxidative Stress; D011110:Polymorphism, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048249:Streptococcus thermophilus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics; D024483:Vitamin K 3 / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D015534:Trans-Activators; C074779:rgg protein, Streptococcus; D024483:Vitamin K 3