title: The transcription factor Egr-1 is a regulator of the human TopBP1 gene.
abstract: The human topoisomerase IIbeta binding protein 1 (TopBP1) has been reported to be involved in DNA replication, in DNA damage checkpoints and in apoptosis. Detailed analysis of the TopBP1 promoter revealed that the early growth response protein-1 (Egr-1) induces this promoter. Binding of Egr-1 to the TopBP1 promoter was determined to region -50 to -18 using EMSA and ChIP technology. Furthermore, deletion of the E2F transcription factor binding sites or mutation of the Egr-1 transcription factor binding sites lead to reduced stimulation of the TopBP1 promoter by Egr-1. These data indicate a cooperative regulation of the TopBP1 promoter by Egr-1 and E2F.
authors.affiliation: Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute), Beutenbergstrasse 11a, D-07745 Jena, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Usskilat
authors.forename: Christine
authors.initials: C
authors.lastname: Skerka
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Saluz
authors.forename: Frank
authors.initials: F
authors.lastname: Hänel
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002352:Carrier Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D050684:E2F Transcription Factors / Q000378:metabolism / Q000502:physiology; D051766:Early Growth Response Protein 1 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D019476:Insect Proteins / Q000494:pharmacology; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000502:physiology; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic; D050436:Regulatory Elements, Transcriptional; D014157:Transcription Factors; D015533:Transcriptional Activation
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D019476:Insect Proteins; D009687:Nuclear Proteins; C110513:TOPBP1 protein, human; D014157:Transcription Factors