title: Somatic mitochondrial DNA mutations in prostate cancer and normal appearing adjacent glands in comparison to age-matched prostate samples without malignant histology.
abstract: Studies of somatic mitochondrial DNA mutations have become an important aspect of cancer research because these mutations might have functional significance and/or serve as a biosensor for tumor detection. Here we report somatic mitochondrial DNA mutations from three specific tissue types (tumor, adjacent benign, and distant benign) recovered from 24 prostatectomy samples. Needle biopsy tissue from 12 individuals referred for prostate biopsy, yet histologically benign (symptomatic benign), were used as among individual control samples. We also sampled blood (germplasm tissue) from each patient to serve as within individual controls relative to the somatic tissues sampled (malignant, adjacent, and distant benign). Complete mitochondrial genome sequencing was attempted on each sample. In contrast to both control groups [within patient (blood) and among patient (symptomatic benign)], all of the tissue types recovered from the malignant group harbored significantly different mitochondrial DNA (mtDNA) mutations. We conclude that mitochondrial genome mutations are an early indicator of malignant transformation in prostate tissue. These mutations occur well before changes in tissue histo-pathology, indicative of prostate cancer, are evident to the pathologist.
authors.affiliation: Genesis Genomics Inc., 1294 Balmoral St., Thunder Bay, Ontario, Canada, P7B 5Z5. ryan.parr@genesisgenomics.com
authors.forename: Ryan L
authors.initials: RL
authors.lastname: Parr
authors.forename: Gabriel D
authors.initials: GD
authors.lastname: Dakubo
authors.forename: Keith A
authors.initials: KA
authors.lastname: Crandall
authors.forename: Jennifer
authors.initials: J
authors.lastname: Maki
authors.forename: Brian
authors.initials: B
authors.lastname: Reguly
authors.forename: Andrea
authors.initials: A
authors.lastname: Aguirre
authors.forename: Roy
authors.initials: R
authors.lastname: Wittock
authors.forename: Kerry
authors.initials: K
authors.lastname: Robinson
authors.forename: Jude S
authors.initials: JS
authors.lastname: Alexander
authors.forename: Mark A
authors.initials: MA
authors.lastname: Birch-Machin
authors.forename: Mamdouh
authors.initials: M
authors.lastname: Abdel-Malak
authors.forename: M Kent
authors.initials: MK
authors.lastname: Froberg
authors.forename: Eleftherios P
authors.initials: EP
authors.lastname: Diamandis
authors.forename: Robert E
authors.initials: RE
authors.lastname: Thayer
mesh_terms: D000368:Aged; D044963:Biopsy, Fine-Needle; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010802:Phylogeny; D010865:Pilot Projects; D011467:Prostate / Q000166:cytology; D011470:Prostatic Hyperplasia / Q000175:diagnosis / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial