Comparative crystallization and preliminary X-ray diffraction studies of locked nucleic acid and RNA stems of a tenascin C-binding aptamer.
The pharmacokinetic properties of an aptamer against the tumour-marker protein tenascin-C have recently been successfully improved by the introduction of locked nucleic acids (LNAs) into the terminal stem of the aptamer. Since it is believed that this post-SELEX optimization is likely to provide a more general route to enhance the in vitro and in vivo stability of aptamers, elucidation of the structural basis of this improvement was embarked upon. Here, the crystallographic and X-ray diffraction data of the isolated aptamer stem encompassed in a six-base-pair duplex both with and without the LNA modification are presented. The obtained all-LNA crystals belong to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 52.80, c = 62.83 angstroms; the all-RNA crystals belong to space group R32, with unit-cell parameters a = b = 45.21, c = 186.97 angstroms, gamma = 120.00 degrees.
Förster
Charlotte
C
Institute of Chemistry and Biochemistry, Free University Berlin, Thielallee 63, 14195 Berlin, Germany.
Brauer
Arnd B E
AB
Brode
Svenja
S
Schmidt
Kathrin S
KS
Perbandt
Markus
M
Meyer
Arne
A
Rypniewski
Wojciech
W
Betzel
Christian
C
Kurreck
Jens
J
Fürste
Jens P
JP
Erdmann
Volker A
VA
D001665:Binding Sites; D003460:Crystallization; D009696:Nucleic Acids / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D012313:RNA / Q000737:chemistry*; D019063:Tenascin / Q000737:chemistry* / Q000302:isolation & purification; D014961:X-Ray Diffraction
D009696:Nucleic Acids; D010446:Peptide Fragments; D019063:Tenascin; D012313:RNA