Proteoliposome derived cochleate as novel adjuvant.
Cochleate structures (CS) consist in a highly stable lipid structures that have been reported to be a good antigen delivery system. The incorporation of pathogen associated molecular pattern (PAMP) from bacterial membranes into CS became in a promising approach to develop adjuvants, particularly mucosal adjuvants. Therefore, we prepare CS from proteoliposome (PL) obtained from Neisseria meningitidis B (PLCS) and evaluated it for its capability to stimulate the immune system as well as the adjuvant activity. The ability of PLCS to induce Thl polarization was also explored. The results and the easy capability for new antigen incorporation on CS support its use as adjuvant for immunization with a large variety of pathogen derived antigens and different routes of immunization.
Bracho
Gustavo
G
Immunology Department, Finlay Institute, P.O. Box 16017, Havana, Cuba. gbracho@finlay.edu.cu
Lastre
Miriam
M
del Campo
Judith
J
Zayas
Caridad
C
González
Domingo
D
Gil
Danay
D
Acevedo
Reinaldo
R
Taboada
Carlos
C
Solís
Rosa L
RL
Pérez
Oliver
O
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009345:Neisseria meningitidis / Q000737:chemistry / Q000276:immunology; D011510:Proteolipids / Q000737:chemistry / Q000276:immunology*
D000276:Adjuvants, Immunologic; D011510:Proteolipids; C020485:proteoliposomes