[Preparative separation of aloin diastereoisomers by high-speed countercurrent chromatography combined with silica gel column chromatography].
Aloin, naturally a mixture of two diastereoisomers, aloin A and aloin B, is the major anthraquinone in aloe, and now served as one of the important control constituents in most of the commercial aloe products. High-speed countercurrent chromatography (HSCCC) combined with silica gel column chromatography was developed for the preparative separation of the two individual aloins. Aloin A (98%) and aloin B (96%) were obtained. Fast atom bombardment mass spectrometry (FAB-MS), 1H nuclear magnetic resonance (1H NMR) and GOESY (gradient-enhanced nuclear Overhauser effect spectroscopy) were employed for the elucidation of their structure conformation. The developed method is of high preparative capacity and high efficiency in resolution.
Huang
Danfeng
D
Beijing Key Laboratory of Plant Resources Research and Development, School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100037, China.
Cao
Xueli
X
Zhao
Hua
H
Dong
Yinmao
Y
D000504:Aloe / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004642:Emodin / Q000031:analogs & derivatives* / Q000145:classification / Q000302:isolation & purification; D012015:Reference Standards; D058428:Silica Gel; D017640:Silicates / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*
D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D017640:Silicates; C487012:silica hydride; D058428:Silica Gel; D012822:Silicon Dioxide; D004642:Emodin; C006457:alloin