title: Changes in enzymes associated with energy metabolism during the early post mortem period in longissimus thoracis bovine muscle analyzed by proteomics.
abstract: Changes in metabolic protein levels in biopsies during the early post mortem period in the bovine longissimus thoracis muscle were investigated by 2-DE based proteome analyses. Nine NRF (Norwegian Red) dual purpose bulls were included in the study. Twenty-four proteins underwent changes between the two sampling times and were classified into two major groups: metabolic proteins and heat shock proteins. Of the metabolic proteins, 5 enzymes involved in the glycolytic pathway and the tricarboxylic acid (TCA) cycle, increased in intensities during the post mortem period. In addition, the NADP-dependent enzyme 3-hydroxyisobutyrate dehydrogenase, associated with the TCA cycle in muscle, was increased. This documents that an increased aerobic energy metabolism occurs immediately after slaughter, with the aim to replenish the ATP levels in the muscle.
authors.affiliation: Matforsk AS, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Jia
authors.forename: Kjell I
authors.initials: KI
authors.lastname: Hildrum
authors.forename: Frank
authors.initials: F
authors.lastname: Westad
authors.forename: Eiliv
authors.initials: E
authors.lastname: Kummen
authors.forename: Laila
authors.initials: L
authors.lastname: Aass
authors.forename: Kristin
authors.initials: K
authors.lastname: Hollung
mesh_terms: D000818:Animals; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D008297:Male; D008954:Models, Biological; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D016622:Silver Staining; D013997:Time Factors
chemical_list: D009124:Muscle Proteins; D020543:Proteome