Crystallization and preliminary X-ray diffraction analysis of an anti-H(O) lectin from Lotus tetragonolobus seeds.
The seed lectin from Lotus tetragonolobus (LTA) has been crystallized. The best crystals grew over several days and were obtained using the vapour-diffusion method at a constant temperature of 293 K. A complete structural data set was collected at 2.00 angstroms resolution using a synchrotron-radiation source. LTA crystals were found to be monoclinic, belonging to space group P2(1), with unit-cell parameters a = 68.89, b = 65.83, c = 102.53 angstroms, alpha = gamma = 90, beta = 92 degrees. Molecular replacement yielded a solution with a correlation coefficient and R factor of 34.4 and 51.6%, respectively. Preliminary analysis of the molecular-replacement solution indicates a new quaternary association in the LTA structure. Crystallographic refinement is under way.
Moreno
Frederico Bruno Mendes Batista
FB
Programa de Pós-graduação em Biofísica Molecular, Departamento de Física, UNESP, São José do Rio Preto, SP 15054-000, Brazil.
Martil
Daiana Evelin
DE
Cavada
Benildo Sousa
BS
de Azevedo
Walter Filgueira
WF
D003460:Crystallization; D000070116:Lotus / Q000737:chemistry*; D008958:Models, Molecular; D037121:Plant Lectins / Q000737:chemistry* / Q000302:isolation & purification; D020836:Protein Structure, Quaternary; D012639:Seeds / Q000737:chemistry*; D013816:Thermodynamics; D014961:X-Ray Diffraction
D037121:Plant Lectins