title: Role of CaBud6p in the polarized growth of Candida albicans.
abstract: Bud6p is a component of a polarisome that controls cell polarity in Saccharomyces cerevisiae. In this study, we investigated the role of the Candida albicans Bud6 protein (CaBud6p) in cell polarity and hyphal development. CaBud6p, which consists of 703 amino acids, had 37% amino-acid sequence identity with the Bud6 protein of S. cerevisiae. The homozygous knock-out of CaBUD6 resulted in several abnormal phenotypes, such as a round and enlarged cells, widened bud necks, and a random budding pattern. In hypha-inducing media, the mutant cells had markedly swollen tips and a reduced ability to switch from yeast to hypha. In addition, a yeast two-hybrid analysis showed a physical interaction between CaBud6p and CaAct1p, which suggests that CaBud6p may be involved in actin cable organization, like Bud6p in S. cerevisiae. Taken together, these results indicate that CaBud6 plays an important role in the polarized growth of C. albicans.
authors.affiliation: School of Bioscience and Biotechnology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: Yunkyoung
authors.initials: Y
authors.lastname: Song
authors.forename: Jeong-Yoon
authors.initials: JY
authors.lastname: Kim
mesh_terms: D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D002176:Candida albicans / Q000235:genetics / Q000254:growth & development*; D016764:Cell Polarity; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D025301:Hyphae / Q000254:growth & development*; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D000199:Actins; C107727:BUD6 protein, S cerevisiae; D005656:Fungal Proteins; D008840:Microfilament Proteins; D029701:Saccharomyces cerevisiae Proteins