Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels.
An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms.
Izzo
Vincenzo
V
Istituto di Biomedicina e Immunologia Molecolare, C.N.R., Via U. La Malfa, 153, 90153 Palermo, Italy. enzoizzo1948@libero.it
Costa
Maria A
MA
Di Fiore
Renata
R
Duro
Giovanni
G
Bellavia
Daniele
D
Cascone
Eleonora
E
Colombo
Paolo
P
Gioviale
Maria C
MC
Barbieri
Rainer
R