title: TGF-beta1 gene polymorphisms in periodontal diseases.
abstract: BACKGROUND
  Genetic polymorphisms in the TGF-beta1 gene were shown to interfere with the transcriptional activity of the TGF-beta1 gene, and this influences the production, secretion or activity of the TGF-beta1 growth factor. Transforming growth factor-beta1 (TGF-beta1) gene polymorphism is associated with risk of inflammatory diseases.
  
  
  OBJECTIVE
  The aim of this study was to evaluate TGF-beta1 gene polymorphisms in a Turkish population with different periodontal diseases and to investigate the association between TGF-beta1 genotype and clinical periodontal parameters.
  
  
  METHODS
  A total of 134 subjects were included in this study. Genomic DNA was isolated from 51 patients with chronic periodontitis (CP), 43 with generalized aggressive periodontitis (G-AgP) and 40 healthy controls. Three TGF-beta1 gene polymorphisms were identified by PCR-RFLP and MS-PCR at positions +915G/C, Thr263Ile and 713/8delC. Probing pocket depth (PPD), clinical attachment loss (CAL), plaque accumulation (plaque %) and bleeding of probing were obtained. Chi-square, Mann-Whitney U test and logistic regression analysis were used.
  
  
  RESULTS
  There was a slightly significant difference in +915C positive genotype distributions between CP and control groups (OR: 2.46, CI: 1.010-6.005, p = 0.047). No significant differences were present between G-AgP and controls in +915C positive genotype. Thr263Ile and -713/8delC genotype distributions were not different between study groups. There were significant differences in PPD and CAL scores between +915C positive and negative CP patients.
  
  
  CONCLUSIONS
  These findings suggest that the TGF-beta1 (+915C) polymorphic allele might be associated with chronic periodontitis in the Turkish population.
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, Bornova-35100, Izmir, Turkey. gulatilla@yahoo.com <gulatilla@yahoo.com>
authors.forename: Gül
authors.initials: G
authors.lastname: Atilla
authors.forename: Gülnur
authors.initials: G
authors.lastname: Emingil
authors.forename: Timur
authors.initials: T
authors.lastname: Köse
authors.forename: Afig
authors.initials: A
authors.lastname: Berdeli
mesh_terms: D000328:Adult; D000483:Alleles; D002908:Chronic Disease; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D010510:Periodontal Diseases / Q000235:genetics*; D010518:Periodontitis / Q000235:genetics*; D011110:Polymorphism, Genetic; D012044:Regression Analysis; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014421:Turkey
chemical_list: D016212:Transforming Growth Factor beta