A conjugate vaccine composed of a heat shock protein 60 T-cell epitope peptide (p458) and Neisseria meningitidis type B capsular polysaccharide.
Neisseria meningitidis type B is a major world-health problem. The Meningococcus type B capsular polysaccharide (MnB) is very poorly immunogenic and no vaccine to the antigen exists. Here, we conjugated the MnB to a T-cell carrier peptide (p458) derived from the self-60kDa heat shock protein molecule. The conjugate vaccine was effective in inducing long-lasting IgG antibodies to the MnB antigen in mice. The vaccine was also immunogenic when injected in PBS. Thus, the p458 carrier peptide can induce T-cell help for the switch to IgG Ab to the MnB antigen.
Amir-Kroll
Hila
H
Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel.
Riveron
Luis
L
Sarmiento
Maria E
ME
Sierra
Gustavo
G
Acosta
Armando
A
Cohen
Irun R
IR
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D018834:Chaperonin 60 / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005260:Female; D007115:Immunization Schedule; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D038541:Neisseria meningitidis, Serogroup B / Q000276:immunology*; D038542:Neisseria meningitidis, Serogroup C / Q000276:immunology; D010455:Peptides / Q000276:immunology; D011134:Polysaccharides / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000276:immunology
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D018834:Chaperonin 60; D000939:Epitopes; D007074:Immunoglobulin G; D010455:Peptides; D011134:Polysaccharides; D018074:Vaccines, Conjugate