[Analysis of differential protein expression profile between retinoic acid resistant and sensitive acute promyelocytic leukemia cells].
OBJECTIVE
This study was to compare the protein expression profiles between retinoic acid (RA) resistant and sensitive acute promyelocytic leukemia (APL) cells by proteomic research method.


METHODS
Total cellular proteins extracted from a RA sensitive cell line NB4 and a RA resistant cell line MR2 were separated by two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE). High quality 2D-PAGE protein profiles were obtained and analyzed by PDQuest v7.1 analysis software to screen differentially expressed protein spots. Those sports were identified by mass spectrometry.


RESULTS
2-DE patterns of APL cell lines with high-resolution and reproducibility were obtained. The average spots for MR2 and NB4 cells were 890+/-45 and 912+/-56, respectively. 57 significantly differentially expressed protein spots were screened, among which 23 protein spots were identified to be up-regulated and 34 down-regulated in MR2 cells compared with NB4 cells. Ten proteins were identified by mass spectrometry, with a successful identification rate of 70%. The identified proteins could be classified into different categories: oncogenes,cell cycle regulator and signal transducer.


CONCLUSIONS
The utilization of 2D-PAGE is effective in identifying the protein expression profiles between RA resistant and sensitive APL cells, therefore this study may provide a novel clue to elucidate the drug resistant mechanisms of all-trans retinoic acid.
Qin
Hui
H
Department of Hematology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China. qinhui6957@sina.com
Liu
Ting
T
Yang
Jin-Liang
JL
Huang
Xin
X
Liu
Bin
B
Song
Xin
X
Zhao
Xia
X
Wei
Yu-Quan
YQ
D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D018834:Chaperonin 60 / Q000032:analysis; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000032:analysis*; D015513:Oncogene Proteins / Q000032:analysis; D000091402:Prohibitins; D000071617:Protein Deglycase DJ-1; D040901:Proteomics / Q000379:methods*; D012097:Repressor Proteins / Q000032:analysis; D014212:Tretinoin / Q000494:pharmacology*
D000970:Antineoplastic Agents; D018834:Chaperonin 60; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D015513:Oncogene Proteins; D000091402:Prohibitins; D012097:Repressor Proteins; D014212:Tretinoin; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1