title: Red palm oil suppresses the formation of azoxymethane (AOM) induced aberrant crypt foci (ACF) in Fisher 344 male rats.
abstract: Red palm oil (RPO) contains significant levels of carotenoids and Vitamin E. In this experiment we compared the inhibitory effects of RPO (7% and 14% levels) and soybean oil (7% and 14%) on azoxymethane (AOM) induced aberrant crypt foci (ACF). Thirty-two male Fisher 344 rats were randomly assigned to four groups. Two groups received AIN-93 G control (C) diet containing 7% and 14% soybean oil (SBO), respectively. Groups 3 and 4 received a treatment diet consisting of 7% and 14% RPO, respectively. The rats received subcutaneous injections of AOM at 16 mg/kg body weight at 7 and 8 weeks of age. At 17 weeks of age rats were killed by CO(2) asphyxiation. Numbers of ACF (mean+/-SE) in the proximal and distal colon were: 39.9 +/- 0.9, 53.8 +/- 2.8, 26.0 +/- 3.0, 27.5 +/- 1.5 and 118.2 +/- 1.7, 125.6 +/- 3.2, 41 +/- 7, 52.3 +/- 1.8 in rats fed 7% SBO, 14% SBO, 7% RPO and 14% RPO, respectively. The results of this study showed that RPO reduced the incidence of AOM induced ACF and may therefore have a beneficial effect in reducing the incidence of colon cancer.
authors.affiliation: Nutrition Biochemistry and Carcinogenesis Laboratory, Department of Food and Animal Sciences, Alabama A&M University, P.O. Box 1628, Normal, 35762, USA.
authors.forename: J
authors.initials: J
authors.lastname: Boateng
authors.forename: M
authors.initials: M
authors.lastname: Verghese
authors.forename: C B
authors.initials: CB
authors.lastname: Chawan
authors.forename: L
authors.initials: L
authors.lastname: Shackelford
authors.forename: L T
authors.initials: LT
authors.lastname: Walker
authors.forename: J
authors.initials: J
authors.lastname: Khatiwada
authors.forename: D S
authors.initials: DS
authors.lastname: Williams
mesh_terms: D000818:Animals; D001397:Azoxymethane; D002338:Carotenoids / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D004435:Eating / Q000187:drug effects; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D000073878:Palm Oil; D010938:Plant Oils / Q000494:pharmacology*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000517:prevention & control*; D051381:Rats; D011916:Rats, Inbred F344; D013024:Soybean Oil / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology; D015430:Weight Gain / Q000187:drug effects
chemical_list: D010938:Plant Oils; D014810:Vitamin E; D002338:Carotenoids; D000073878:Palm Oil; D013024:Soybean Oil; D005982:Glutathione Transferase; D001397:Azoxymethane