Cutting edge: unique T cells that recognize citrullinated peptides are a feature of protein immunization.
Abs against citrullinated proteins are present in patients with rheumatoid arthritis. In this study, we describe a unique cohort of T cells that selectively responded to citrullinated variants of two epitopes of hen egg-white lysozyme, a major and a minor one, bound to the MHC molecule, I-A(k). In addition, we show that when given an intact, unmodified lysozyme protein, dendritic cells and peritoneal macrophages presented citrullinated peptides and stimulated modification-specific T cells. Thus, presentation of citrullinated-peptide-MHC complex is a feature of immune responses to protein Ags.
Ireland
Jamie
J
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Herzog
Jeremy
J
Unanue
Emil R
ER
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002956:Citrulline / Q000276:immunology* / Q000378:metabolism*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology / Q000378:metabolism; D005620:Freund's Adjuvant / Q000008:administration & dosage; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009113:Muramidase / Q000008:administration & dosage / Q000276:immunology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D022223:Vaccines, Subunit / Q000008:administration & dosage* / Q000276:immunology* / Q000378:metabolism
D018984:Epitopes, T-Lymphocyte; D010446:Peptide Fragments; D022223:Vaccines, Subunit; D002956:Citrulline; D005620:Freund's Adjuvant; C421864:hen egg lysozyme; D009113:Muramidase