title: Brain tissue characterisation by infrared imaging in a rat glioma model.
abstract: Pathological changes associated with the development of brain tumor were investigated by Fourier transform infrared microspectroscopy (FT-IRM) with high spatial resolution. Using multivariate statistical analysis and imaging, all normal brain structures were discriminated from tumor and surrounding tumor tissues. These structural changes were mainly related to qualitative and quantitative changes in lipids (tumors contain little fat) and were correlated to the degree of myelination, an important factor in several neurodegenerative disorders. Lipid concentration and composition may thus be used as spectroscopic markers to discriminate between healthy and tumor tissues. Additionally, we have identified one peculiar structure all around the tumor. This structure could be attributed to infiltrative events, such as peritumoral oedema observed during tumor development. Our results highlight the ability of FT-IRM to identify the molecular origin that gave rise to the specific changes between healthy and diseased states. Comparison between pseudo-FT-IRM maps and histological examinations (Luxol fast blue, Luxol fast blue-cresyl violet staining) showed the complementarities of both techniques for early detection of tissue abnormalities.
authors.affiliation: Unité MéDIAN, CNRS-UMR 6142, UFR de Pharmacie, IFR 53, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096 Reims cedex, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Amharref
authors.forename: Abdelilah
authors.initials: A
authors.lastname: Beljebbar
authors.forename: Sylvain
authors.initials: S
authors.lastname: Dukic
authors.forename: Lydie
authors.initials: L
authors.lastname: Venteo
authors.forename: Laurence
authors.initials: L
authors.lastname: Schneider
authors.forename: Michel
authors.initials: M
authors.lastname: Pluot
authors.forename: Richard
authors.initials: R
authors.lastname: Vistelle
authors.forename: Michel
authors.initials: M
authors.lastname: Manfait
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D001921:Brain / Q000473:pathology*; D001932:Brain Neoplasms / Q000175:diagnosis / Q000473:pathology*; D003952:Diagnostic Imaging / Q000379:methods*; D004195:Disease Models, Animal; D005910:Glioma / Q000175:diagnosis / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D014408:Biomarkers, Tumor