Digital imaging of the human cornea in vivo with ultraviolet light.
We have a developed a novel and practical method of imaging the cornea under ultraviolet (UV) light using a digital medium. Wavelengths of 336-371 nm were used to illuminate the cornea. Images were recorded using a UV Nikkor lens and a digital charged coupled device (CCD). Images obtained showed ferritin lines not visible under white light. This study concluded that 336-371 nm is comparable to shorter wavelengths for the imaging of ferritin in the cornea and that a digital image capture system was comparable to that of film.
Aird
J J
JJ
Section of Ophthalmology, Department of Medical and Surgical Sciences, University of Otago Dunedin School of Medicine, New Zealand.
Molteno
A C B
AC
Bevin
T H
TH
Sanderson
G
G
Wynn-Williams
G
G
D003315:Cornea / Q000033:anatomy & histology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D019721:Ophthalmoscopes; D009887:Ophthalmoscopy / Q000379:methods*; D010781:Photography / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D014466:Ultraviolet Rays