title: Cross-inhibitory interactions between GABAA and P2X channels in myenteric neurones.
abstract: Inhibitory interactions between GABA(A)[induced by gamma-aminobutyric acid (GABA)] and P2X [activated by adenosine 5'-triphosphate (ATP)] receptors of myenteric neurones from the guinea pig small intestine were characterized using whole-cell recordings. Currents induced by GABA (I(GABA)) or ATP (I(ATP)) were inhibited by picrotoxin or pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid, respectively. Currents induced by GABA + ATP (I(GABA+ATP)) were only as large as the current induced by the most effective transmitter, revealing current occlusion. This occlusion requires maximal activation of at least one of these receptors. Sequential applications of neurotransmitters, and kinetic and pharmacological properties of I(GABA+ATP) indicate that they are carried through both GABA(A) and P2X channels. ATP did not affect I(GABA) in neurones: (i) in which P2X channels were not present; (ii) after inhibiting P2X channels with Ca2+ (iii) in the presence of pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid, a P2X receptor antagonist; (iv) after P2X receptor desensitization or (v) at I(ATP) reversal potential. Similarly, GABA did not affect P2X-mediated currents in neurones: (i) in which GABA(A) channels were not present; (ii) in the presence of picrotoxin, a GABA(A) channel blocker; (iii) after GABA(A) receptor desensitization or (iv) at the I(GABA) reversal potential. Current occlusion occurred as fast as current activation and it was still present in the absence of Ca2+, at 11 degrees C, after adding to the pipette solution a cocktail of protein kinase inhibitors (staurosporine + genistein + K-252a), after substituting the GTP in the pipette with GDP-beta-S and after treating the cells with N-ethylmaleimide. Taken together, all of these results are consistent with a model of cross-inhibition between GABA(A) and P2X.
authors.affiliation: Department of Anatomy & Cell Biology, Queen's University, Kingston, Ontario, Canada.
authors.forename: Rustum
authors.initials: R
authors.lastname: Karanjia
authors.forename: Luz M
authors.initials: LM
authors.lastname: García-Hernández
authors.forename: Marcela
authors.initials: M
authors.lastname: Miranda-Morales
authors.forename: Nureen
authors.initials: N
authors.lastname: Somani
authors.forename: Rosa
authors.initials: R
authors.lastname: Espinosa-Luna
authors.forename: Luis M
authors.initials: LM
authors.lastname: Montaño
authors.forename: Carlos
authors.initials: C
authors.lastname: Barajas-López
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D005260:Female; D018756:GABA Antagonists / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D006168:Guinea Pigs; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D010852:Picrotoxin / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism*; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D058469:Receptors, Purinergic P2X; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D000255:Adenosine Triphosphate; D002118:Calcium; D018756:GABA Antagonists; D019204:GTP-Binding Proteins; D010852:Picrotoxin; D011732:Pyridoxal Phosphate; D011963:Receptors, GABA-A; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D005680:gamma-Aminobutyric Acid; C077792:pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid