Expression of matrix metalloproteinases and aggrecanase in the synovial fluids of patients with symptomatic temporomandibular disorders.
OBJECTIVE
To investigate whether matrix metalloproteinases (MMPs) and/or aggrecanase in synovial fluid can be used as biochemical markers in the diagnosis of internal derangement (ID) of the temporomandibular joint (TMJ).


METHODS
Forty-four samples of synovial fluid were obtained from 35 patients with ID and osteoarthritis (OA) and 15 normal samples from 10 asymptomatic volunteers. MMP-2, -9, and aggrecanase in the synovial fluid were examined by immunoblotting.


RESULTS
The incidences of MMP-2, -9, and aggrecanase expression in the ID and OA group were significantly higher than those in the normal group (P < .05). Those with anterior disc displacement without reduction and severe OA showed significantly high expression of MMP-9 compared with other disease subgroups (P < .05). Conversely, comparatively high expression of MMP-2 and aggrecanase was shown in the early-stage OA group. However, there was no significant difference in expression of MMP-2 and aggrecanase among disease subgroups.


CONCLUSIONS
These findings suggested that expression of aggrecanase could be a potential biochemical marker for articular cartilage degradation in ID of the TMJ.
Yoshida
Kan
K
Department of Oral and Maxillofacial Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-8640, Japan. kany@oral.m.kanazawa-u.ac.jp
Takatsuka
Shigeyuki
S
Hatada
Eriko
E
Nakamura
Hiroyuki
H
Tanaka
Akira
A
Ueki
Koichiro
K
Nakagawa
Kiyomasa
K
Okada
Yasunori
Y
Yamamoto
Etsuhide
E
Fukuda
Ryuuzi
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers; D015153:Blotting, Western; D002358:Cartilage, Articular / Q000201:enzymology; D016022:Case-Control Studies; D010450:Endopeptidases / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D004204:Joint Dislocations / Q000201:enzymology; D008279:Magnetic Resonance Imaging; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology; D019152:Paracentesis; D013582:Synovial Fluid / Q000737:chemistry / Q000201:enzymology*; D013705:Temporomandibular Joint Disorders / Q000201:enzymology* / Q000473:pathology
D015415:Biomarkers; D010450:Endopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C083641:aggrecanase