title: Bonding durability of a self-etching primer system to normal and caries-affected dentin under hydrostatic pulpal pressure in vitro.
abstract: OBJECTIVE
  To evaluate the bonding durability of a self-etching primer system to normal and caries-affected dentin under hydrostatic pulpal pressure.
  
  
  METHODS
  18 extracted human molars with occlusal caries were used. Their occlusal dentin surfaces were ground flat to expose normal and caries-affected dentin using #600 SiC paper under running water. Clearfil SE Bond was placed on the dentin surface including the caries-affected dentin according to the manufacturer's instructions and then the crowns were built up with resin composite (Clearfil AP-X) under either a pulpal pressure of 15 cm H2O or none (control). The bonded specimens were stored in 100% humidity for 1 day (control) or for 1 week and 1 month with hydrostatic pulpal pressure. After storage, the specimens were serially sectioned into 0.7 mm-thick slabs and trimmed to an hour-glass shape with a 1 mm2 cross-section, isolated by normal or caries-affected dentin, and then subjected to the micro-tensile bond test. Data were analyzed by two-way ANOVA and Tukey's test (P< 0.05).
  
  
  RESULTS
  Hydrostatic pulpal pressure significantly reduced the bond strength to normal dentin after 1-month storage (P< 0.05), but did not affect the bond strength to caries-affected dentin.
authors.affiliation: FEAD-Minas School of Dentistry, Belo Horizonte, Brazil. nakajima.ope@tmd.ac.jp
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nakajima
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hosaka
authors.forename: Monica
authors.initials: M
authors.lastname: Yamauti
authors.forename: Richard M
authors.initials: RM
authors.lastname: Foxton
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D000704:Analysis of Variance; D003188:Composite Resins; D001840:Dental Bonding; D003731:Dental Caries; D003782:Dental Pulp / Q000502:physiology; D003799:Dental Stress Analysis; D003804:Dentin; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents; D019234:Dentinal Fluid; D006801:Humans; D006874:Hydrostatic Pressure; D008422:Materials Testing; D019279:Resin Cements; D018709:Statistics, Nonparametric; D013718:Tensile Strength
chemical_list: C438313:Clearfil SE Bond; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D019279:Resin Cements