Effect of direct infusion of acetaldehyde on dopamine and dopamine-derived salsolinol in the striatum of free-moving rats using a reverse microdialysis technique.
The effects of acetaldehyde (ACD) on dopamine (DA) and DA-derived salsolinol (SAL) levels were investigated in the striatum of freely moving rats. Dialysate levels of DA and SAL were determined using in vivo reverse microdialysis coupled with high-performance liquid chromatography with an electrochemical detector. Perfusion with 1,000 microM ACD decreased DA levels significantly, as compared to baseline value, whereas 250 and 500 microM ACD perfusion did not result in any significant alteration of the DA levels in the striatal dialysates. SAL levels in the dialysates were determined first at 30 or 40 min after ACD perfusion, reached a peak at 150 min, followed by no alterations for 240 min with doses of 250, 500, and 1,000 microM ACD. Our in vivo study suggested that 1,000 microM ACD led to significant decreases in DA levels in the striatum with greater SAL formation, and the examined ACD concentrations induced a dose-dependent elevation in SAL levels in the striatum of freely moving rats.
Wang
Weihuan
W
Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan. weihuanw@kms.ac.jp
Ameno
Kiyoshi
K
Jamal
Mostofa
M
Kumihashi
Mitsuru
M
Uekita
Ikuo
I
Ameno
Setsuko
S
Ijiri
Iwao
I
D000079:Acetaldehyde / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects*; D004298:Dopamine / Q000494:pharmacology*; D004347:Drug Interactions; D004359:Drug Therapy, Combination; D007546:Isoquinolines / Q000494:pharmacology*; D008297:Male; D017551:Microdialysis / Q000379:methods; D009068:Movement / Q000502:physiology; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
D007546:Isoquinolines; C036617:salsolinol; D000079:Acetaldehyde; D004298:Dopamine