title: Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB.
abstract: A 16S rRNA gene database (http://greengenes.lbl.gov) addresses limitations of public repositories by providing chimera screening, standard alignment, and taxonomic classification using multiple published taxonomies. It was found that there is incongruent taxonomic nomenclature among curators even at the phylum level. Putative chimeras were identified in 3% of environmental sequences and in 0.2% of records derived from isolates. Environmental sequences were classified into 100 phylum-level lineages in the Archaea and Bacteria.
authors.affiliation: Center for Environmental Biotechnology, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mail Stop 70A-3317, Berkeley, CA 94720, USA.
authors.forename: T Z
authors.initials: TZ
authors.lastname: DeSantis
authors.forename: P
authors.initials: P
authors.lastname: Hugenholtz
authors.forename: N
authors.initials: N
authors.lastname: Larsen
authors.forename: M
authors.initials: M
authors.lastname: Rojas
authors.forename: E L
authors.initials: EL
authors.lastname: Brodie
authors.forename: K
authors.initials: K
authors.lastname: Keller
authors.forename: T
authors.initials: T
authors.lastname: Huber
authors.forename: D
authors.initials: D
authors.lastname: Dalevi
authors.forename: P
authors.initials: P
authors.lastname: Hu
authors.forename: G L
authors.initials: GL
authors.lastname: Andersen
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics; D001419:Bacteria / Q000145:classification / Q000235:genetics; D030561:Databases, Nucleic Acid / Q000592:standards*; D020459:Genes, rRNA; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D011995:Recombination, Genetic; D015203:Reproducibility of Results; D016415:Sequence Alignment; D012984:Software
chemical_list: D012336:RNA, Ribosomal, 16S