title: Nano- and microparticles as adjuvants in vaccine design: success and failure is related to host natural antibodies.
abstract: Bovine serum albumin (BSA) and the surface A-layer protein (AP) of an atypical strain of fish bacterial pathogen Aeromonas salmonicida were covalently linked with polymeric nano- and microparticles, and antigenicity of the resulted conjugates was compared in mice and goldfish. Distinct albeit different levels of natural BSA and AP antibodies were present in both animal species. Significant stimulation of the anti-AP antibody response in mice strikingly contrasted to unresponsiveness or even suppression in fish. The results negatively correlate with the levels of respective natural antibodies in the host and are discussed in context of problems related to fish vaccination. The work reinforces the instructive role of natural antibodies in adaptive immune response.
authors.affiliation: Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel. sinyakov@mail.biu.ac.il
authors.forename: Michael S
authors.initials: MS
authors.lastname: Sinyakov
authors.forename: Moti
authors.initials: M
authors.lastname: Dror
authors.forename: Tammy
authors.initials: T
authors.lastname: Lublin-Tennenbaum
authors.forename: Samuel
authors.initials: S
authors.lastname: Salzberg
authors.forename: Shlomo
authors.initials: S
authors.lastname: Margel
authors.forename: Ramy R
authors.initials: RR
authors.lastname: Avtalion
mesh_terms: D000171:Acrolein / Q000737:chemistry / Q000276:immunology; D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D048409:Aeromonas salmonicida / Q000254:growth & development / Q000276:immunology; D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology*; D002417:Cattle; D002626:Chemistry, Pharmaceutical; D015195:Drug Design; D004655:Emulsions; D006054:Goldfish / Q000276:immunology; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000276:immunology; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D000906:Antibodies; D000941:Antigens; D000942:Antigens, Bacterial; D004655:Emulsions; D007202:Indicators and Reagents; D011108:Polymers; D014612:Vaccines; C029975:polyacrolein; D012710:Serum Albumin, Bovine; D000171:Acrolein; D012685:Sepharose