Expression changes of ERK1/2, STAT3 and SHP-2 in bone marrow cells from gamma-ray induced leukemia mice.
The aim is to clarify expression changes of ERK1/2, STAT3 and SHP-2 in bone marrow cells from gamma-ray induced leukemia mice. A mouse model of gamma-ray induced leukemia was produced, and by means of quantitative real-time PCR, immunoprecipitation, Western blotting and electrophoretic mobility shift assays (EMSA), the expression of mRNA and protein, phosphorylation level, and protein activity of ERK1/2, STAT3 and SHP-2 in bone marrow cells were investigated in these mice. The results indicated that mRNA and protein expressions of ERK1/2 were upregulated, with significant increase of phosphorylation level and protein activity, but with insignificant differences in mRNA and protein expressions, phosphorylation level and protein activity of STAT3 and SHP-2 in bone marrow cells from gamma-ray induced leukemia mice compared to the radiation/tumor-free or control mice. It is concluded that in the pathogenesis of gamma-ray induced leukemia in Balb/C mice, activated ERK1/2 pathway may play a role, without involving STAT3 pathway; meanwhile, SHP-2 exerts no regulative effect on pathways of Ras-ERK1/2 and JAK-STAT.
Fu
Zhichao
Z
Department of Radiotherapy, General Hospital of Fuzhou Military Command, China.
Huang
Dingde
D
Cai
Jianming
J
Chen
Qi
Q
Han
Ling
L
Li
Bailong
B
Wang
Fengmei
F
Gao
Jianguo
J
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000528:radiation effects*; D002478:Cells, Cultured; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D007953:Leukemia, Radiation-Induced / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D014916:Whole-Body Irradiation / Q000009:adverse effects
D047908:Intracellular Signaling Peptides and Proteins; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D019950:Mitogen-Activated Protein Kinase 1; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D017027:Protein Tyrosine Phosphatases; C516725:Ptpn11 protein, mouse