title: A genetically encoded fluorescent amino acid.
abstract: The fluorescent amino acid l-(7-hydroxycoumarin-4-yl) ethylglycine 1 has been genetically encoded in E. coli in response to the amber TAG codon. Because of its high fluorescence quantum yield, relatively large Stoke's shift, and sensitivity to both pH and polarity, this amino acid should provide a useful probe of protein localization and trafficking, protein conformation changes, and protein-protein interactions.
authors.affiliation: Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Jiangyun
authors.initials: J
authors.lastname: Wang
authors.forename: Jianming
authors.initials: J
authors.lastname: Xie
authors.forename: Peter G
authors.initials: PG
authors.lastname: Schultz
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D000818:Animals; D001665:Binding Sites; D018388:Codon, Terminator; D019020:Directed Molecular Evolution; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D005453:Fluorescence; D005815:Genetic Code; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008968:Molecular Conformation; D009211:Myoglobin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D050661:Sperm Whale; D014468:Umbelliferones / Q000737:chemistry* / Q000378:metabolism
chemical_list: C522545:(7-hydroxycoumarin-4-yl) ethylglycine; D000596:Amino Acids; D018388:Codon, Terminator; D029968:Escherichia coli Proteins; D009211:Myoglobin; D014468:Umbelliferones; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D005998:Glycine