Crystal structure of glycoprotein B from herpes simplex virus 1.
Glycoprotein B (gB) is the most conserved component of the complex cell-entry machinery of herpes viruses. A crystal structure of the gB ectodomain from herpes simplex virus type 1 reveals a multidomain trimer with unexpected homology to glycoprotein G from vesicular stomatitis virus (VSV G). An alpha-helical coiled-coil core relates gB to class I viral membrane fusion glycoproteins; two extended beta hairpins with hydrophobic tips, homologous to fusion peptides in VSV G, relate gB to class II fusion proteins. Members of both classes accomplish fusion through a large-scale conformational change, triggered by a signal from a receptor-binding component. The domain connectivity within a gB monomer would permit such a rearrangement, including long-range translocations linked to viral and cellular membranes.
Heldwein
Ekaterina E
EE
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA. heldwein@crystal.harvard.edu
Lou
Huan
H
Bender
Florent C
FC
Cohen
Gary H
GH
Eisenberg
Roselyn J
RJ
Harrison
Stephen C
SC
D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D000939:Epitopes; D019143:Evolution, Molecular; D018259:Herpesvirus 1, Human / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000502:physiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry; D014721:Vesicular stomatitis Indiana virus / Q000737:chemistry; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000276:immunology / Q000502:physiology; D014760:Viral Fusion Proteins / Q000737:chemistry*
D000939:Epitopes; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D021122:Protein Subunits; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C040495:glycoprotein B, Simplexvirus