title: A DHA14 drug efflux gene from Xanthomonas albilineans confers high-level albicidin antibiotic resistance in Escherichia coli.
abstract: OBJECTIVE
  Identification of a gene for self-protection from the antibiotic-producing plant pathogen Xanthomonas albilineans, and functional testing by heterologous expression.
  
  
  RESULTS
  Albicidin antibiotics and phytotoxins are potent inhibitors of prokaryote DNA replication. A resistance gene (albF) isolated by shotgun cloning from the X. albilineans albicidin-biosynthesis region encodes a protein with typical features of DHA14 drug efflux pumps. Low-level expression of albF in Escherichia coli increased the MIC of albicidin 3000-fold, without affecting tsx-mediated albicidin uptake into the periplasm or resistance to other tested antibiotics. Bioinformatic analysis indicates more similarity to proteins involved in self-protection in polyketide-antibiotic-producing actinomycetes than to multi-drug resistance pumps in other gram-negative bacteria. A complex promoter region may co-regulate albF with genes for hydrolases likely to be involved in albicidin activation or self-protection.
  
  
  CONCLUSIONS
  AlbF is the first apparent single-component antibiotic-specific efflux pump from a gram-negative antibiotic producer. It shows extraordinary efficiency as measured by resistance level conferred upon heterologous expression.
  
  
  CONCLUSIONS
  Development of the clinical potential of albicidins as potent bactericidial antibiotics against diverse bacteria has been limited because of low yields in culture. Expression of albF with recently described albicidin-biosynthesis genes may enable large-scale production. Because albicidins are X. albilineans pathogenicity factors, interference with AlbF function is also an opportunity for control of the associated plant disease.
authors.affiliation: Department of Botany, SIB, The University of Queensland, Brisbane, Australia.
authors.forename: J M
authors.initials: JM
authors.lastname: Bostock
authors.forename: G
authors.initials: G
authors.lastname: Huang
authors.forename: S M
authors.initials: SM
authors.lastname: Hashimi
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: R G
authors.initials: RG
authors.lastname: Birch
mesh_terms: D001483:Base Sequence; D019149:Bioreactors; D004351:Drug Resistance / Q000235:genetics*; D004926:Escherichia coli / Q000502:physiology*; D015870:Gene Expression; D015723:Gene Library; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014974:Xanthomonas / Q000235:genetics* / Q000378:metabolism
chemical_list: D009930:Organic Chemicals; C046156:albicidin