title: Desaturase mutants reveal that membrane rigidification acts as a cold perception mechanism upstream of the diacylglycerol kinase pathway in Arabidopsis cells.
abstract: Membrane rigidification could be the first step of cold perception in poikilotherms. We have investigated its implication in diacylglycerol kinase (DAGK) activation by cold stress in suspension cells from Arabidopsis mutants altered in desaturase activities. By lateral diffusion assay, we showed that plasma membrane rigidification with temperature decrease was steeper in cells deficient in oleate desaturase than in wild type cells and in cells overexpressing linoleate desaturase. The threshold for the activation of the DAGK pathway in each type of cells correlated with this order of rigidification rate, suggesting that cold induced-membrane rigidification is upstream of DAGK pathway activation.
authors.affiliation: Centre National de la Recherche Scientifique, Université Pierre et Marie Curie-Paris 6, FRE 2846, PCMP, 3 Rue Galilée, F-94200 Ivry-sur-Seine, France.
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Vaultier
authors.forename: Catherine
authors.initials: C
authors.lastname: Cantrel
authors.forename: Chantal
authors.initials: C
authors.lastname: Vergnolle
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Justin
authors.forename: Chantal
authors.initials: C
authors.lastname: Demandre
authors.forename: Ghouziel
authors.initials: G
authors.lastname: Benhassaine-Kesri
authors.forename: Dominique
authors.initials: D
authors.lastname: Ciçek
authors.forename: Alain
authors.initials: A
authors.lastname: Zachowski
authors.forename: Eric
authors.initials: E
authors.lastname: Ruelland
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D019852:Diacylglycerol Kinase / Q000235:genetics / Q000378:metabolism*; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism; D008560:Membrane Fluidity / Q000502:physiology*; D009154:Mutation; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D044943:Fatty Acid Desaturases; D019852:Diacylglycerol Kinase