Base-base interactions in the minor groove of double-stranded DNA.
A method has been developed for the synthesis of bisheaded nucleosides with thymine and adenine base moieties. We have demonstrated that, when incorporated in oligonucleotides, extrahelical A-T base interactions are possible when the bisheaded nucleosides are positioned in opposite strands of the duplex and are separated from each other by one regular base pair.
Wu
Tongfei
T
Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
Nauwelaerts
Koen
K
Van Aerschot
Arthur
A
Froeyen
Matheus
M
Lescrinier
Eveline
E
Herdewijn
Piet
P
D000225:Adenine / Q000737:chemistry*; D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D013941:Thymine / Q000737:chemistry*
D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides; D004247:DNA; D000225:Adenine; D013941:Thymine