Survey and properties of Staphylococcus aureus isolated from Japanese-style desserts.
We surveyed the contamination of 315 Japanese- and western-style desserts and 247 human hands by Staphylococcus aureus and other staphylococcal bacteria. The most frequently isolated staphylococcal bacterium was S. warneri, followed by S. aureus. Only 1.9% of western-style desserts were contaminated by S. aureus strains, while 19.4% and 13.0% of Japanese-style desserts and human hands respectively were contaminated. Ninety-four isolates of S. aureus were characterized as to their biological properties and enterotoxigenicity. Although staphylococcal enterotoxins (SEs) were detected by enzyme-linked immunosorbent assay in the cultured broth of all S. aureus isolates, the reversed passive latex agglutination method and the polymerase chain reaction showed only 39 (41.5%) and 40 (42.6%) samples respectively as SE-positive. The predominant type of SE was SEB (67.5%), and eight strains produced SEA. None of the S. aureus strains had penicillin-binding protein 2', showing that methicillin-resistant S. aureus was not present in the samples.
Shimamura
Yuko
Y
Department of Nutrition and Food Science, Ochanomizu University, Tokyo 112-8610, Japan.
Kidokoro
Shiho
S
Murata
Masatsune
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004768:Enterotoxins / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005516:Food Microbiology; D006225:Hand / Q000382:microbiology; D006801:Humans; D007841:Latex Fixation Tests; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins / Q000032:analysis; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D004768:Enterotoxins; D046915:Penicillin-Binding Proteins; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal