title: Paenibacillus pasadenensis sp. nov. and Paenibacillus barengoltzii sp. nov., isolated from a spacecraft assembly facility.
abstract: Two novel spore-forming, Gram-positive, mesophilic, heterotrophic bacteria representing two novel species were isolated from the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF) at Pasadena, CA, USA. The incidence of similar strains was examined by screening the growing collection of isolates ( approximately 400 strains) obtained from the JPL-SAF using species-specific PCR primer sets designed from the 16S rRNA gene sequences of strains SAFN-016(T) and SAFN-007(T). Phylogenetic analysis of 16S rRNA gene sequences placed these novel isolates within the genus Paenibacillus. Two strains, SAFN-016(T) and SAFN-125, shared 98 % 16S rRNA gene sequence similarity with Paenibacillus timonensis and 97 % similarity with Paenibacillus macerans. Strain SAFN-007(T) showed 95.2 % 16S rRNA gene sequence similarity with Paenibacillus kobensis, its nearest phylogenetic neighbour. The results of DNA-DNA hybridization, physiological tests and biochemical analysis allowed genotypic and phenotypic differentiation of the isolates from currently recognized Paenibacillus species. Strain SAFN-007(T) and strains SAFN-016(T) and SAFN-125 are representatives of two separate novel species, for which the names Paenibacillus pasadenensis sp. nov. (type strain SAFN-007(T)=ATCC BAA-1211(T)=NBRC 101214(T)) and Paenibacillus barengoltzii sp. nov. (type strain SAFN-016(T)=ATCC BAA-1209(T)=NBRC 101215(T)) are proposed.
authors.affiliation: Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, 89-2; Biotechnology and Planetary Protection Group, 4800, Oak Grove Dr., Pasadena, CA 91109, USA.
authors.forename: Shariff
authors.initials: S
authors.lastname: Osman
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, 236-8648, Japan.|Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, 89-2; Biotechnology and Planetary Protection Group, 4800, Oak Grove Dr., Pasadena, CA 91109, USA.
authors.forename: Masataka
authors.initials: M
authors.lastname: Satomi
authors.affiliation: Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, 89-2; Biotechnology and Planetary Protection Group, 4800, Oak Grove Dr., Pasadena, CA 91109, USA.
authors.forename: Kasthuri
authors.initials: K
authors.lastname: Venkateswaran
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004780:Environment, Controlled; D004783:Environmental Microbiology; D020459:Genes, rRNA; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D018531:Spacecraft; D013171:Spores, Bacterial; D014481:United States
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S