[Investigation of CXCR4 in oral squamous cell carcinoma and its influence on proliferation of tumor cells in vitro].
OBJECTIVE
To evaluate the expression of CXCR4 in oral squamous cell carcinoma (OSCC) and its influence on the proliferation of OSCC cells.


METHODS
The expression of CXCR4 was evaluated by immunohistochemical study in 91 surgically resected oral squamous cell carcinoma, and the relation between the staining and clinicopathological features was examined. Flow cytometry was used to detect CXCR4 expression in oral squamous cell carcinoma cell line. MTT assay was used to evaluate the SDF-1/CXCR4 influence on proliferation of tumor cells.


RESULTS
The expression of CXCR4 in OSCC was 62.6% and there was a significant association between the expression of CXCR4 and lymph node metastases (P < 0.05), tumor size (P < 0.01) and tumor histology grade (P < 0.01). In flow cytometry, a significant shift in fluorescent staining was seen in cells. In MTT assay, recombinant SDF-1 stimulated proliferation of OSCC cell and CXCR4 neutralization by monoclonal antibodies decreased proliferation.


CONCLUSIONS
The expression of CXCR4 was significantly correlated with the clinicopathological features and tumor proliferation. CXCR4 might be a valuable biomarker to predict the progression of OSCC.
Xu
Meng
M
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
Li
Wu-yi
WY
Chen
Xin-ming
XM
Wang
Xin-hong
XH
Huang
Jing
J
Wang
Li
L
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology*; D019718:Receptors, CXCR4 / Q000378:metabolism*
C527276:CXCR4 protein, human; D019718:Receptors, CXCR4