Regulatory components at the csgD promoter--additional roles for OmpR and integration host factor and role of the 5' untranslated region.
CsgD is a master regulator of multicellular behaviour in Salmonella enterica serovar Typhimurium. Expression of CsgD is highly regulated on the transcriptional level. A nucleo-protein complex had been defined where the global regulators OmpR and integration host factor (IHF) bind up- and downstream of the csgD core promoter. In this study, the nucleo-protein complex of PcsgD was extended through characterization of additional OmpR and IHF binding sites that influence the transcriptional activity of the csgD promoter. Furthermore, the role of the 174 bp long 5'-untranslated region on transcriptional activity was defined.
Gerstel
Ulrich
U
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
Kolb
Annie
A
Römling
Ute
U
D020121:5' Untranslated Regions / Q000502:physiology*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D001483:Base Sequence; D001665:Binding Sites; D018983:DNA Footprinting; D021901:DNA, Intergenic / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D034982:Integration Host Factors / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D015534:Trans-Activators / Q000378:metabolism / Q000502:physiology*
D020121:5' Untranslated Regions; D001426:Bacterial Proteins; D021901:DNA, Intergenic; D034982:Integration Host Factors; D015534:Trans-Activators; C440508:integration host factor, Salmonella; C112177:osmolarity response regulator proteins