The DefH9-iaaM-containing construct efficiently induces parthenocarpy in cucumber.
Parthenocarpy (seedless fruits) is a desirable trait that has been achieved in many plant cultivars. We generated parthenocarpic cucumber fruits by introducing the chimeric DefH9-iaaM construct into the cucumber genome using an Agrobacterium tumefaciens-mediated protocol. The construct consists of the DefH9 promoter from Antirrhinum majus and the iaaM coding sequence from Pseudomonas syringae. Transgenic plants were obtained from nine independent transformation events: half of these were tetraploid and did not produce seeds following self-pollination, while the remaining half were capable of displaying parthenocarpy in the subsequent reproductive generation. Of the fruits produced by the transgenic lines, 70-90% were parthenocarpic. The segregation of the marker gene in the transgenic T(1) progeny indicated single gene inheritance. The seed set in the transgenic lines and their F(1) hybrids was lower than in the non-transgenic control plants. Some of the methodological details and the practical significance of the results are discussed.
Yin
Zhimin
Z
Institute of Plant Genetics, Polish Academy of Science, Strzeszyńska 34, Poznań, Poland.
Malinowski
Robert
R
Ziółkowska
Agnieszka
A
Sommer
Hans
H
Plcader
Wojciech
W
Malepszy
Stefan
S
D015139:Blotting, Southern; D003433:Crosses, Genetic; D018553:Cucumis sativus / Q000235:genetics* / Q000502:physiology; D005434:Flow Cytometry; D005638:Fruit / Q000254:growth & development*; D005798:Genes, Bacterial; D030821:Plants, Genetically Modified; D011058:Pollen / Q000502:physiology; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012038:Regeneration; D012639:Seeds / Q000502:physiology; D019076:Transgenes