title: [Effects of nitric oxide synthases on kidney in early phase of endotoxic shock in macaque].
abstract: OBJECTIVE
  To study the pathogenesis of kidney injury in early phase of endotoxic shock.
  
  
  METHODS
  Eleven macaques were anesthetized and randomly divided into two groups: an experimental group (n=6), receiving a dose of 2.8 mg/kg lipopolysaccharides (LPS) by intravenous injection, and a control group (n=5), injected with normal saline of 1 ml/kg. The animals were sacrificed 120 minutes following endotoxin injection. With immunohistochemical technique endothelium nitric oxide synthases (eNOS) and inducible nitric oxide synthases (iNOS) protein of the renal tissue were determined. The ultrastructure was studied with electron microscope. Acid phosphatase (ACP) was also assayed.
  
  
  RESULTS
  One hundred and twenty minutes after LPS challenge, damages to the glomerulus and renal tubules were found, and lysosomes were increased in the renal cells. iNOS was positively expressed in vascular endothelium of glomerulus and epithelium of renal tubules in the experimental group, but not in the control group. eNOS was positively expressed in vascular endothelium of glomerulus; and peri-vascular region in both groups.
  
  
  CONCLUSIONS
  The present findings indicate that NO contributed to the pathogenesis of renal injury in the early phase of endotoxic shock in primates.
authors.affiliation: Department of Medicine, The Second Hospital of Nanjing, Medical College, South East University, Nanjing 210003, Jiangsu, China. maming_1971@yahoo.com.cn
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Zhu
authors.forename: Li-rong
authors.initials: LR
authors.lastname: Wang
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Gu
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Yin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007668:Kidney / Q000201:enzymology / Q000473:pathology*; D008070:Lipopolysaccharides / Q000633:toxicity; D008251:Macaca; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D011897:Random Allocation; D012772:Shock, Septic / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology*
chemical_list: D008070:Lipopolysaccharides; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III