Sonoporation, anti-cancer drug and antibody delivery using ultrasound.
It has been shown experimentally in cell suspensions that sonoporation could be used to deliver the anti-cancer drug Adriamycin hydrochloride (ADR) into Jurkat lymphocytes and that it could also be used to deliver goat anti-rabbit IgG and anti-mouse IgD, antibodies into human peripheral blood mononuclear cells (PBMC) and Jurkat lymphocytes. When ADR was delivered, the delivery efficiency reached 4.80+/-2.04% and control baseline (no ultrasound and no ADR) was 0.17+/-0.14%. When anti-rabbit IgD was delivered, the efficiencies were 34.90+/-1.80% (control baseline was 1.33+/-0.31%) and 32.50+/-4.36% (control baseline was 1.66+/-0.06%) respectively for Jurkat cells and PBMC. When goat anti-rabbit IgG was delivered, the efficiencies were 78.60+/-3.60% (control baseline was 1.60+/-0.00%) and 57.50+/-4.23% (control baseline was 11.30+/-0.81%) respectively for Jurkat cells and PBMC.
Wu
Junru
J
Department of Physics, University of Vermont, Burlington, VT 05405, USA. Jun-Ru.Wu@uvm.edu <Jun-Ru.Wu@uvm.edu>
Pepe
Jason
J
Rincón
Mercedes
M
D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000906:Antibodies / Q000008:administration & dosage / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000528:radiation effects*; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D019169:Jurkat Cells; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D015612:Phonophoresis / Q000379:methods*
D000903:Antibiotics, Antineoplastic; D000906:Antibodies