title: Platelet-dependent thrombography: a method for diagnostic laboratories.
abstract: The thrombin-platelet feedback loop was examined at low concentration tissue factor using calibrated automated thrombography in combination with the elimination of contact factor activation by corn trypsin inhibitor. The results indicated that, when contact factor activation was eliminated, the thrombin-platelet feedback loop was a major determinant of thrombin generating capacity and that platelets had a greater role in regulating the propagation of thrombin generation than its initiation. This method has potential application to the measurement of platelet-dependent thrombin generation in clinical diagnostic laboratories and hence the investigation of patients with apparent hypo- or hypercoagulable phenotypes.
authors.affiliation: Department of Haematology, Addenbrooke's NHS Trust, Cambridge, UK.
authors.forename: Yesim
authors.initials: Y
authors.lastname: Dargaud
authors.forename: Roger
authors.initials: R
authors.lastname: Luddington
authors.forename: Trevor
authors.initials: T
authors.lastname: Baglin
mesh_terms: D000328:Adult; D019540:Area Under Curve; D001792:Blood Platelets / Q000378:metabolism*; D016022:Case-Control Studies; D025461:Feedback, Physiological; D005260:Female; D006467:Hemophilia A / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D013917:Thrombin / Q000032:analysis / Q000096:biosynthesis*; D013925:Thromboplastin / Q000378:metabolism*; D013997:Time Factors
chemical_list: D013925:Thromboplastin; D013917:Thrombin