Bacterial derived proteoliposome for allergy vaccines.
One current approach in developing anti allergic vaccines is the use of potent adjuvants, capable of inducing Th1 or T regulatory cells. Proteoliposomes (PL) could be a suitable adjuvant. Purified Dermatophagoides siboney (Ds) allergens were mixed with PL and adsorbed into Al(OH)3 and evaluated in mice. The Th1/Th2 responses were measured at classes, subclasses, cytokines, and DTH levels. Anti Ds response was deviated to a Thl pattern, with the production of IgG2a and gamma1FN. A positive DTH response and a dramatic decrease of specific IgE and IL5 were not detected. The low dose was more effective than high dose. These results clearly support the potential use of PL as possible adjuvants for anti-allergic vaccines.
Lastre
Miriam
M
Immunology Department, Finlay Institute, P.O. Box 16017, Havana, Cuba. mlastre@finlay.edu.cu
Pérez
Oliver
O
Labrada
Alexis
A
Bidot
Igor
I
Pérez
Jorge
J
Bracho
Gustavo
G
del Campo
Judith
J
Pérez
Dainerys
D
Facenda
Elisa
E
Zayas
Caridad
C
Rodríguez
Claudio
C
Sierra
Gustavo
G
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage / Q000276:immunology*; D006967:Hypersensitivity / Q000517:prevention & control*; D051379:Mice; D038541:Neisseria meningitidis, Serogroup B / Q000276:immunology; D011510:Proteolipids / Q000008:administration & dosage / Q000276:immunology*; D014612:Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D039741:Antigens, Dermatophagoides; D011510:Proteolipids; D014612:Vaccines; C020485:proteoliposomes; D000536:Aluminum Hydroxide