ENA Actimineral Resource A restores bone loss and bone quality in ovariectomized rats.
The purpose of this study was to examine the effects of ENA Actimineral Resource A (ENA-A), seaweed origin alkaline water, on postmenopausal osteoporosis in ovariectomized (OVX) rats. The 12-week old Wistar rats were divided randomly into 4 groups: ovariectomized (OVX), OVX plus 0.5% ENA-A, OVX plus 5% ENA-A and OVX plus 10% ENA-A. A histopathological analysis indicated that ENA-A could prevent OVX-induced bone loss by increasing femur trabecular bone area in a dose-dependent manner. ENA-A significantly (p<0.05) increased serum estradiol levels, decreased serum osteocalcin activity and suppressed serum pyridinoline (PYD) levels. The in vitro effects of ENA-A were also studied using MC3T3-E1 cells. ENA-A significantly stimulated cell proliferation and increased both ALP activity and calcium deposition in a dose-dependent manner. These results suggest that the treatment of ovariectomized rats with ENA-A not only prevents bone resorption but also appears to maintain the cancellous bone structure of postmenopausal osteoporosis.
Do
Sun Hee
SH
Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, #1370 Sangyeok-dong, Buk-gu, Daegu, 702-701, Republic of Korea.
Hwa
Sung-Yong
SY
Jeong
Won-Il
WI
Lee
Ji-Won
JW
Kim
Hyun-Jung
HJ
Lee
In-Seon
IS
Jeong
Kyu-Shik
KS
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000596:Amino Acids / Q000097:blood; D000818:Animals; D001835:Body Weight; D001862:Bone Resorption / Q000628:therapy*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D049109:Cell Proliferation; D004958:Estradiol / Q000097:blood; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D008900:Mineral Waters / Q000627:therapeutic use*; D008903:Minerals / Q000627:therapeutic use*; D015675:Osteocalcin / Q000097:blood; D010010:Osteoclasts / Q000166:cytology; D010012:Osteogenesis; D010052:Ovariectomy; D028321:Plant Preparations / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000596:Amino Acids; C518516:ENA Actimineral Resource A; D008900:Mineral Waters; D008903:Minerals; D028321:Plant Preparations; D012333:RNA, Messenger; D015675:Osteocalcin; D004958:Estradiol; C015484:pyridinoline; D000469:Alkaline Phosphatase