title: Evaluation of the resazurin microtiter assay for rapid detection of ofloxacin resistance in M. tuberculosis.
abstract: OBJECTIVE
  To evaluate the performance of the colorimetric resazurin microtiter assay (REMA) method for the detection of ofloxacin resistance.
  
  
  METHODS
  A panel of 120 multidrug-resistant Mycobacterium tuberculosis strains was tested blindly by the REMA method and compared with the results obtained using the BACTEC 460 method.
  
  
  RESULTS
  A very good correlation was observed between the two methods.
  
  
  CONCLUSIONS
  The REMA method is simple, rapid and can be an inexpensive alternative procedure for the rapid detection of anti-tuberculosis drug resistance in laboratories with limited resources.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medecine, Antwerp, Belgium. alainenyaruhirira@hotmail.com
authors.forename: A N
authors.initials: AN
authors.lastname: Umubyeyi
authors.forename: A
authors.initials: A
authors.lastname: Martin
authors.forename: G
authors.initials: G
authors.lastname: Zissis
authors.forename: M
authors.initials: M
authors.lastname: Struelens
authors.forename: E
authors.initials: E
authors.lastname: Karita
authors.forename: F
authors.initials: F
authors.lastname: Portaels
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D015242:Ofloxacin / Q000494:pharmacology*; D010078:Oxazines / Q000737:chemistry*; D014966:Xanthenes / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D015242:Ofloxacin