The effect of acteoside on histamine release and arachidonic acid release in RBL-2H3 mast cells.
The effect of acteoside, a phenylpropanoid glycoside isolated from Clerodendron trichotomum Thunberg, on histamine and arachidonic acid release was investigated in RBL 2H3 cells. Histamine was dose-dependently released from RBL 2H3 cells by melittin, arachidonic acid and thapsigargin. In extracellular Ca2+-free solution, basal secretion of histamine increased by two fold. The response of histamine release to melittin and thapsigargin in Ca2+-free solution was significantly decreased, whereas the response to arachidonic acid was significantly increased as compared with those in normal solution. Acteoside inhibited histamine release induced by melittin, arachidonic acid and thapsigargin in a dose-dependent manner in the presence or absence of extracellular Ca2+. However, the inhibitory activity of acteoside was more potent in normal solution than that in Ca2+-free solution. These data suggest that inhibitory mechanism of acteoside on histamine release may be related to extracellular Ca2+. On the other hand, acteoside significantly inhibited arachidonic acid release and prostaglandin E2 production induced by 0.5 microM melittin. It is possible that acteoside may be developed as an anti-inflammatory agent.
Lee
Jin Hee
JH
College of Pharmacy, Chung-Ang University, Seoul, Korea.
Lee
Ji Yun
JY
Kang
Hyo Suk
HS
Jeong
Chan Hun
CH
Moon
Hee
H
Whang
Wan Kyunn
WK
Kim
Chang Jong
CJ
Sim
Sang Soo
SS
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism; D045744:Cell Line, Tumor; D032409:Clerodendrum / Q000737:chemistry*; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D020536:Enzyme Activators / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006636:Histamine Release; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology; D008555:Melitten / Q000494:pharmacology; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010741:Phospholipases A / Q000378:metabolism; D051381:Rats; D019284:Thapsigargin / Q000494:pharmacology; D059808:Polyphenols
D000893:Anti-Inflammatory Agents; D016718:Arachidonic Acid; D002118:Calcium; D000252:Calcium-Transporting ATPases; D015232:Dinoprostone; D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D005960:Glucosides; D008555:Melitten; D010636:Phenols; D010741:Phospholipases A; D019284:Thapsigargin; C058956:acteoside; D059808:Polyphenols