Identification of the ETT2 locus in human diarrheagenic Escherichia coli by multiplex PCR.
An Escherichia coli type III secretion system 2 (ETT2) locus was discovered in enterohemorrhagic E. coli O157:H7. To determine presence or absence of the ETT2 locus in diarrheagenic E. coli, a multiplex polymerase chain reaction (PCR) encoding Shiga toxins (stx1 and stx2), intimin (eaeA), and ETT2 (etrA) was developed for rapid detection. The ETT2 locus was identified not only in Shiga toxin-producing E. coli (STEC) but also in various non-STEC.
Osawa
Kayo
K
Department of Medical Technology, Faculty of Health Sciences, Kobe University School of Medicine, 7-10-2 Tomogaoka, Suma-ku, Kobe, 654-0142, Japan. osawak@kobe-u.ac.jp
Shibata
Mariko
M
Nishiyama
Yoko
Y
Kurokawa
Manabu
M
Yamamoto
Go
G
Kinoshita
Shohiro
S
Kataoka
Nobumasa
N
D003967:Diarrhea / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*