Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms.
Shewanella oneidensis MR-1 produced electrically conductive pilus-like appendages called bacterial nanowires in direct response to electron-acceptor limitation. Mutants deficient in genes for c-type decaheme cytochromes MtrC and OmcA, and those that lacked a functional Type II secretion pathway displayed nanowires that were poorly conductive. These mutants were also deficient in their ability to reduce hydrous ferric oxide and in their ability to generate current in a microbial fuel cell. Nanowires produced by the oxygenic phototrophic cyanobacterium Synechocystis PCC6803 and the thermophilic, fermentative bacterium Pelotomaculum thermopropionicum reveal that electrically conductive appendages are not exclusive to dissimilatory metal-reducing bacteria and may, in fact, represent a common bacterial strategy for efficient electron transfer and energy distribution.
Gorby
Yuri A
YA
Pacific Northwest National Laboratory, Richland, WA 99352, USA. yuri.gorby@pnl.gov
Yanina
Svetlana
S
McLean
Jeffrey S
JS
Rosso
Kevin M
KM
Moyles
Dianne
D
Dohnalkova
Alice
A
Beveridge
Terry J
TJ
Chang
In Seop
IS
Kim
Byung Hong
BH
Kim
Kyung Shik
KS
Culley
David E
DE
Reed
Samantha B
SB
Romine
Margaret F
MF
Saffarini
Daad A
DA
Hill
Eric A
EA
Shi
Liang
L
Elias
Dwayne A
DA
Kennedy
David W
DW
Pinchuk
Grigoriy
G
Watanabe
Kazuya
K
Ishii
Shun'ichi
S
Logan
Bruce
B
Nealson
Kenneth H
KH
Fredrickson
Jim K
JK
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics; D003574:Cytochrome c Group / Q000235:genetics; D004553:Electric Conductivity; D004583:Electrons; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D016296:Mutagenesis; D009154:Mutation; D036103:Nanotechnology; D020592:Shewanella / Q000378:metabolism* / Q000648:ultrastructure*; D046939:Synechocystis / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D003574:Cytochrome c Group; D005290:Ferric Compounds; C114590:omc A protein, Shewanella putrefaciens; C000499:ferric oxide; C092844:ferric oxyhydroxide; C433527:MtrC protein, Shewanella