title: Identification and characterization of Escherichia coli RS218-derived islands in the pathogenesis of E. coli meningitis.
abstract: BACKGROUND
  Escherichia coli K1 is the most common gram-negative bacterium causing neonatal meningitis, but the mechanisms by which E. coli K1 causes meningitis are not clear.
  
  
  METHODS
  We identified 22 E. coli RS218-derived genomic islands (RDIs), using a comparative genome analysis of meningitis-causing E. coli K1 strain RS218 (O18:K1:H7) and laboratory K-12 strain MG1655. Series of RDI deletion mutants were constructed and examined for phenotypes relevant to E. coli K1 meningitis.
  
  
  RESULTS
  We identified 9 RDI deletion mutants (RDI 1, 4, 7, 12, 13, 16, 20, 21, and 22) that exhibited defects in meningitis development. RDI 16 and 21 mutants had profound defects in the induction of a high level of bacteremia in neonatal rats, and RDI 4 mutants exhibited a moderate defect in the induction of bacteremia. RDI 1 and 22 mutants showed defects in the ability to invade human brain microvascular endothelial cells (HBMECs), and RDI 12 mutants were defective in the ability to bind to HBMECs. RDI 13 and 20 mutants were defective in the ability to both bind to and invade HBMECs. RDI 7 mutants were defective in the induction of bacteremia and in the ability to both bind to and invade HBMECs.
  
  
  CONCLUSIONS
  These results provide a framework for the future discovery and analysis of bacteremia and meningitis caused by E. coli K1 strain RS218.
authors.affiliation: Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Vitaliy
authors.initials: V
authors.lastname: Kolisnychenko
authors.forename: Maneesh
authors.initials: M
authors.lastname: Paul-Satyaseela
authors.forename: Simon
authors.initials: S
authors.lastname: Elliott
authors.forename: Geetha
authors.initials: G
authors.lastname: Parthasarathy
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Yao
authors.forename: Guy
authors.initials: G
authors.lastname: Plunkett
authors.forename: Frederick R
authors.initials: FR
authors.lastname: Blattner
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Kim
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000382:microbiology; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D017353:Gene Deletion; D044404:Genomic Islands; D006801:Humans; D008297:Male; D020814:Meningitis, Escherichia coli / Q000382:microbiology*; D008969:Molecular Sequence Data; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors