Analysis of the role of COMATOSE and peroxisomal beta-oxidation in the determination of germination potential in Arabidopsis.
Comparative physiological analysis of mutant Arabidopsis seeds under defined environmental conditions was used to analyse the relative contributions of components of peroxisomal beta-oxidation in the control of seed germination potential. The COMATOSE (CTS) and KAT2 loci were shown to play essential roles in regulating germination and establishment potentials, whereas LACS6 and LACS7 loci only influenced establishment following germination. The viability and desiccation tolerance of three different mutant alleles of CTS were shown to be intermediate between that of dormant and non-dormant wild-type seeds. Analysis of ttg-1 cts-1 double mutant seeds demonstrated that the cts lesion did not influence after-ripening capacity. These data demonstrate the importance of peroxisomal beta-oxidation in the control of germination potential, but suggest that breakdown of stored lipid is not an important prerequisite for germination. A function is suggested for CTS following after-ripening within pathways related to the progression of germination prior to radicle emergence.
Footitt
Steven
S
Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK.
Marquez
Julietta
J
Schmuths
Heike
H
Baker
Alison
A
Theodoulou
Frederica L
FL
Holdsworth
Michael
M
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000502:physiology*; D000251:Adenosine Triphosphatases; D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003066:Coenzyme A Ligases / Q000378:metabolism / Q000502:physiology; D018525:Germination / Q000502:physiology*; D009154:Mutation; D010084:Oxidation-Reduction; D020675:Peroxisomes / Q000378:metabolism*; D010641:Phenotype; D015221:Potassium Channels / Q000378:metabolism / Q000502:physiology; D024642:Potassium Channels, Voltage-Gated; D012639:Seeds / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; C428126:KAT2 protein, Arabidopsis; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D000251:Adenosine Triphosphatases; C456843:At4g39850 protein, Arabidopsis; D003066:Coenzyme A Ligases; C470374:LACS6 protein, Arabidopsis; C470375:LACS7 protein, Arabidopsis