title: Measurement of antibody binding to protein immobilized on gold nanoparticles by localized surface plasmon spectroscopy.
abstract: Antibody binding to bovine serum albumin (BSA) and human serum albumin (HSA) immobilized onto gold nanoparticles was studied by means of localized surface plasmon resonance (LSPR) spectroscopy. Amine-modified glass was prepared by self-assembly of amine-terminated silane on substrate, and gold (Au) nanoparticles were deposited on the amine-modified glass substrate. Au nanoparticles deposited on the glass surface were functionalized by BSA and HSA. BSA immobilization was confirmed by LSPR spectroscopy in conjunction with surface-enhanced Raman scattering spectroscopy. Then, LSPR response attributable to the binding of anti-BSA and anti-HSA to BSA- and HSA-functionalized Au nanoparticles, respectively, was examined. Anti-HSA at levels larger than approximately 10 nM could be detected by HSA-immobilized chips with LSPR optical response, which was saturated at concentrations greater than approximately 650 nM of anti-HSA.
authors.affiliation: Department of Materials Process & Applied Chemistry for Environments, Faculty of Engineering and Resource Science, Akita University, Tegata Gakuen-cho, Akita, 010-8502, Japan. kfuji@ipc.akita-u.ac.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Fujiwara
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Watarai
authors.forename: Hideaki
authors.initials: H
authors.lastname: Itoh
authors.forename: Erika
authors.initials: E
authors.lastname: Nakahama
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D036103:Nanotechnology / Q000379:methods*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D000906:Antibodies; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D006046:Gold