Urine flow cytometer quantification of leukocytes in samples containing a large proportion of lymphocytes.
OBJECTIVE
We compared leukocyte counts obtained by cytometric analysis and Fuchs-Rosenthal (FR) chamber counting in different proportions of lymphocytes (Lym%) suspensions and cerebrospinal fluid (CSF).


METHODS
UF-100 (UF) was evaluated. For preparation of cell suspensions, gradient density centrifugation method was used.


RESULTS
The regression equation for UF and FR chamber counting of the cell suspensions was y=0.88x+18.8 WBC/microL (r=0.832, n=106). For a few high Lym% samples, markedly underestimated WBC counts were obtained by UF.


CONCLUSIONS
Underestimated WBC count is due not to systematic error but to random error. Counts of the "other" population by UF may be useful for detection of underestimated samples.
Yamanishi
Hachiro
H
Laboratory for Clinical Investigation, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka 565-0871, Japan. yamaha@hp-lab.med.osaka-u.ac.jp
Imai
Nobuko
N
Ohmine
Toshinari
T
Nishiyama
Miho
M
Suehisa
Etsuji
E
Kanakura
Yuzuru
Y
Iwatani
Yoshinori
Y
D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology*