[Comparison of the outcome of intracytoplasmic single sperm injection between two motile sperm separation methods].
OBJECTIVE
To evaluate the efficiency of density gradient and improved swim-up methods for motile sperm isolation from fresh semen samples in intracytoplasmic sperm injection and embryo transfer (ICSI-ET) program, thus guiding the clinical application.


METHODS
The fertilization rate, cleavage rate, high-quality embryo rate, clinical pregnancy rate, sperm abnormal rate and recovery rate of 42 cycles were studied prospectively. The cycles were divided into two groups with respect to the motile sperm isolation methods.


RESULTS
No obvious difference was found in the fertilization rate, cleavage rate, high-quality embryo rate and clinical pregnancy rate between the two methods. The abnormal sperm rate induced by the improved swim-up method was significantly higher than that of the density gradient method (P < 0.01). For severe oligozoospermia, the recovery rate of motile sperm with density gradient was higher than that of improved swim-up (P < 0.01).


CONCLUSIONS
In the ICSI-ET, there is no obvious difference in clinical pregnancy outcome between the two methods. The method of improved swim-up can be used for all patients but those with severe oligozoospermia.
Yang
Hui-jun
HJ
Reproductive Medical Center of Shandong University & Shandong Provincial Hospital, Jinan, Shandong 250021, China.
Li
Yuan
Y
Li
Mei
M
Ma
Shui-ying
SY
Hu
Jing-mei
JM
D000328:Adult; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology*