Characterization of chicken interleukin 2 receptor alpha chain, a homolog to mammalian CD25.
To identify chicken IL-2R alpha chain (chCD25), the cDNA of chCD25 was cloned and mapped onto chicken chromosome 1. The polyclonal and monoclonal antibodies raised from the recombinant chCD25 specifically bound to the cell surface of splenic mononuclear cells (SMC) and inhibited chicken IL-2-dependent proliferation of T cells. Flow cytometry analysis revealed that chCD25 molecules could be expressed on the surface of monocytes/macrophages, thrombocytes, CD4+ and CD8+ cells as well as tissue cells. Importantly, the CD4+CD25+ and CD8+CD25+ cells were upregulated dramatically in chickens infected with H9N2 avian influenza virus. These results confirm that the cloned cDNA is the nucleotide sequence of chicken IL-2R, and suggest that chicken CD4+CD25+ and CD8+CD25+ cells may play an important role in immune responses induced by H9N2 virus, and the monoclonal antibodies to chCD25 may be useful for investigating biological functions of chicken regulatory T cells.
Teng
Qiao-Yang
QY
Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine, Zhejiang University, No. 268 Kaixuan Road, Hangzhou, Zhejiang 310029, PR China.
Zhou
Ji-Yong
JY
Wu
Jia-Jun
JJ
Guo
Jun-Qing
JQ
Shen
Hui-Gang
HG
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology / Q000821:virology; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D002522:Chlorocebus aethiops; D002875:Chromosomes / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology; D005585:Influenza in Birds / Q000235:genetics / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit; D007962:Leukocytes / Q000276:immunology / Q000821:virology; D008969:Molecular Sequence Data; D015375:Receptors, Interleukin-2 / Q000235:genetics* / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D053645:Interleukin-2 Receptor alpha Subunit; D015375:Receptors, Interleukin-2