title: [Regulation of PTHrP in proliferation and differentiation of chondrocytes of condyle in fetal mouse].
abstract: OBJECTIVE
  To investigate the regulatory mechanism of parathyroid hormone-related protein (PTHrP) in proliferation and differentiation of chondrocytes of condyle in fetal mouse.
  
  
  METHODS
  Chondrocytes of condyle in fetal mouse were separated and cultured in vitro, the influence of PTHrP on proliferation and differentiation was observed.
  
  
  RESULTS
  After two weeks' culture in 0.01 nmol/L, 0.1 nmol/L, 1 nmol/L, 10 nmol/L human PTHrP, there was significant difference in the number of cartilage nodule formed between experiment group and control group (P<0.05), and there was no significant difference in 0.01 nmol/L group (P>0.05). Alkaline phosphatase (ALP) activity was significantly intensified in experiment group and control group (P<0.05). Meanwhile, it was found that this function of promotion was lessened after anti-PTHR antibody used.
  
  
  CONCLUSIONS
  It can be seen that PTHrP, via its receptor, can promote proliferation and differentiation of chondrocytes of condyle, which resemble its modulation mechanism in epiphyseal growth plate cartilage intramembrane in mandibule.
authors.affiliation: Dept. of Oral Anatomy and Physiology, College of Stomatology, Nanjing Medical Science University, Nanjing 210029, China.
authors.forename: Lai-Kui
authors.initials: LK
authors.lastname: Liu
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Jiang
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Yin
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Li
authors.forename: Xin-Zhu
authors.initials: XZ
authors.lastname: Yi
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019902:Chondrocytes; D006132:Growth Plate; D006801:Humans; D051379:Mice; D044162:Parathyroid Hormone-Related Protein
chemical_list: D044162:Parathyroid Hormone-Related Protein