Cell-free expression systems for eukaryotic protein production.
Following the success of genome sequencing projects, attention has now turned to studies of the structure and function of proteins. Although cell-based expression systems for protein production have been widely used, they have certain limitations in terms of the quality and quantity of the proteins produced and for high-throughput production. Many of these limitations can be circumvented by the use of cell-free translation systems. Among such systems, the wheat germ based system is of special interest for its eukaryotic nature; it has the significant advantage of producing eukaryotic multidomain proteins in a folded state. Several advances in the use of cell-free expression systems have been made in the past few years and successful applications of these systems to produce proteins for functional and structural biology studies have been reported.
Endo
Yaeta
Y
Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Japan. yendo@eng.ehime-u.ac.jp
Sawasaki
Tatsuya
T
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D002474:Cell-Free System / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D040901:Proteomics / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
D011994:Recombinant Proteins