Identification of microsatellite markers in the rye genome.
The rye genomic library, which consists of DNA fragments in the range of 0.5-1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.
Bolibok
Hanna
H
Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, Nowoursynowska 166, Warszawa, Poland.
Rakoczy-Trojanowska
Monika
M
Wyrzykowska
Małgorzata
M
Radecka
Magdalena
M
Orczyk
Wacław
W
D032461:Chromosomes, Plant / Q000235:genetics; D017931:DNA Primers / Q000378:metabolism; D015723:Gene Library; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D012434:Secale / Q000235:genetics*; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D005819:Genetic Markers