title: Deregulated human glioma cell motility: inhibitory effect of somatostatin.
abstract: Malignant gliomas are highly invasive tumors which are lethal despite aggressive therapy. The motility behavior of two human glioma cell lines i.e. T98G and U87-MG cells was analysed. The glioma cells showed a high degree of basal motility (especially U87-MG cells) that may be related to the considerable local invasiveness of such tumours even in the absence of exogenous factors. The two cell lines responded equally well to platelet-derived growth factor (PDGF) as chemoattractant factor. The phosphatidylinositol 3-kinase (PI3-K) signaling, but not the extracellular signal-related kinase (ERK) signaling, was strongly involved in the PDGF-stimulated glioma cell motility. Somatostatin was capable of inhibiting the migration in both glioma cell lines without affecting crucial targets for motility control like PI3-K and Rac activity. These data suggest that somatostatin, by interfering with a target further downstream to Rac, negatively affects glioma cell motility, and may thus offer a pharmacological approach to controlling the deregulated motility of these aggressive tumoral cells.
authors.affiliation: Department of Pharmacology, School of Medicine, University of Milano, Via Vanvitelli 32, 20129 Milano, Italy.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Cattaneo
authors.forename: Davide
authors.initials: D
authors.lastname: Gentilini
authors.forename: Lucia Maria
authors.initials: LM
authors.lastname: Vicentini
mesh_terms: D002070:Butadienes / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002630:Chemotactic Factors / Q000494:pharmacology; D002867:Chromones / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D009025:Morpholines / Q000378:metabolism; D009362:Neoplasm Metastasis; D009570:Nitriles / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D013004:Somatostatin / Q000494:pharmacology*; D020744:rac GTP-Binding Proteins / Q000378:metabolism
chemical_list: D002070:Butadienes; D002630:Chemotactic Factors; D002867:Chromones; D004791:Enzyme Inhibitors; D048049:Extracellular Signal-Regulated MAP Kinases; D006728:Hormones; D009025:Morpholines; D009570:Nitriles; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010982:Platelet-Derived Growth Factor; D051057:Proto-Oncogene Proteins c-akt; D013004:Somatostatin; D020744:rac GTP-Binding Proteins; C113580:U 0126; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one