Serogroups, K1 antigen, and antimicrobial resistance patterns of Aeromonas spp. strains isolated from different sources in Mexico.
A total of 221 strains of Aeromonas species isolated in Mexico from clinical (161), environmental (40), and food (20) samples were identified using the automated system bioMérieux-Vitek. Antisera for serogroups O1 to 044 were tested using the Shimada and Sakazaki scheme. The K1 antigen was examined using as antiserum the O7:K1C of Escherichia coli. Besides, we studied the antimicrobial patterns according to Vitek AutoMicrobic system. Among the 161 clinical strains 60% were identified as A. hydrophila, 20.4% as A. caviae, and 19.25% as A. veronii biovar sobria. Only A. hydrophila and A. veronii biovar sobria were found in food (55 and 90% respectively) and environmental sources (45 and 10% respectively). Using "O" antisera, only 42.5% (94/221) of the strains were serologically identified, 55% (121/221) were non-typable, and 2.5% (6/221) were rough strains. Twenty-two different serogroups were found, O14, O16, O19, O22, and O34 represented 60% of the serotyped strains. More than 50% of Aeromonas strain examined (112/221) expressed K1 encapsulating antigen; this characteristic was predominant among Aeromonas strains of clinical origin. Resistance to ampicillin/sulbactam and cephazolin was detected in 100 and 67% of Aeromonas strain tested for their susceptibility to antibiotics. In conclusion, antibiotic-resistant Aeromonas species that possess the K1 encapsulating antigen and represent serogroups associated with clinical syndrome in man are not uncommon among Aeromonas strains isolated from clinical, food and environmental sources in Mexico.
Garibay
Ramón I Arteaga
RI
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Apartado Postal CON 174 CP 06400 México, D. F.
Aguilera-Arreola
Ma Guadalupe
MG
Ocañia
Armando Navarro
AN
Cerezo
Silvia Giono
SG
Mendoza
Miroslava Sánchez
MS
López
José Molina
JM
Campos
Carlos Eslava
CE
Cravioto
Alejandro
A
Castro-Escarpulli
Graciela
G
D000333:Aeromonas / Q000145:classification / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000032:analysis*; D016667:Bacterial Capsules; D024881:Drug Resistance, Bacterial; D005516:Food Microbiology; D006801:Humans; D008800:Mexico; D008826:Microbial Sensitivity Tests; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D012703:Serotyping; D014871:Water Microbiology
D000900:Anti-Bacterial Agents; D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; C036962:capsular polysaccharide K1