Functional platelet-activating factor receptors in immature forms of leukemic blasts.
Platelet-activating factor (PAF) is a phospholipid mediator with potent immunoregulatory activities on mature leukocytes. PAF modulates leukocyte cytosolic Ca2+ concentration ([Ca2+]i) through a Gq mediated pathway. We highlight, for the first time, Gq transcripts, PAF receptor (PAF-R) transcripts and protein in blast cells of acute myeloid (AML) and lymphoid (ALL) leukemia patients. PAF stimulated [Ca2+]i in leukemic blast cells; PAF effects being prevented by a specific PAF-R antagonist. In conclusion, functional PAF-R are present in blast cells of patients with acute leukemia; a result that could be of physiologic importance regarding the important effect of PAF on leukocytes maturation and functions.
Reynaud
Stéphane
S
UMR CNRS 6101, Centre National de la Recherche Scientifique, Université de Limoges, France.
Malissein
Emilie
E
Donnard
Magali
M
Bordessoule
Dominique
D
Turlure
Pascal
P
Trimoreau
Franck
F
Denizot
Yves
Y
D000208:Acute Disease; D001752:Blast Crisis; D002118:Calcium / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D007951:Leukemia, Myeloid / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D010980:Platelet Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic
D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D002118:Calcium