title: Endothelial cell-T lymphocyte interactions: IP[corrected]-10 stimulates rapid transendothelial migration of human effector but not central memory CD4+ T cells. Requirements for shear stress and adhesion molecules.
abstract: The chemokine interferon (IFN)-gamma-inducible protein of 10 kDa (IP-10; CXCL10) has been implicated in recruitment of T cells into rejecting allografts yet appears ineffective at stimulating human peripheral blood CD4 T cells to transmigrate across tumor necrosis factor (TNF)-treated human endothelial cell (EC) monolayers in vitro. The same cells rapidly (within 15 min) transmigrate across TNF-treated EC monolayers overlaid with stromal cell-derived factor-1 alpha (SDF-1 alpha) and subjected to shear stress. The effector memory subset within the CD4 T cell population, defined as CD45RO, CD62L and CCR7, which constitutes less than 10% of total CD4 T cells, does respond to IP-10 but requires enrichment to be observed in this model. Central memory T cells do not respond to IP-10. Transendothelial migration of effector memory CD4 T cells requires TNF-pretreatment of the EC monolayer and application of venular shear force during the assay. TNF treatment of ECs may be effectively replaced by transduction of vascular cell adhesion molecule-1 or intercellular adhesion molecule-1 but not E-selectin.
authors.affiliation: Interdepartmental Program in Vascular Biology and Transplantation, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Manes
authors.forename: Jordan S
authors.initials: JS
authors.lastname: Pober
authors.forename: Martin S
authors.initials: MS
authors.lastname: Kluger
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000502:physiology*; D002450:Cell Communication / Q000276:immunology*; D002465:Cell Movement / Q000235:genetics; D002478:Cells, Cultured; D054357:Chemokine CXCL10; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000276:immunology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology*; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000502:physiology; D019041:L-Selectin / Q000032:analysis; D017493:Leukocyte Common Antigens / Q000032:analysis; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000032:analysis; D013314:Stress, Mechanical; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology; D014161:Transduction, Genetic; D048069:Tumor Necrosis Factors / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000502:physiology
chemical_list: C515370:CCR7 protein, human; C515255:CXCL12 protein, human; D015815:Cell Adhesion Molecules; D054357:Chemokine CXCL10; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D048069:Tumor Necrosis Factors; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D017493:Leukocyte Common Antigens