Potential scope of action of tissue kallikreins in CNS immune-mediated disease.
The objective of this study was to define the potential scope of action of tissue kallikreins in T cell-mediated disease of the CNS. We demonstrate quantitatively the differential expression of all 15 human tissue kallikreins within brain, spinal cord and immune compartments. In human Jurkat T cells we demonstrate differential regulation of select kallikreins by CD3 receptor, Concanavilin A (Con A), interleukin 2 (IL2), and lipopolysaccharide (LPS)-mediated activation and by exposure to steroid hormones, dexamethasone, norgestrel, androstan and estradiol. The patterns of co-expression and co-regulation described point to novel effector roles for select tissue kallikreins in neurological disorders involving T cells, such as multiple sclerosis.
Scarisbrick
I A
IA
Department of Physical Medicine and Rehabilitation, Mayo Medical and Graduate Schools, Rochester, MN 55905, USA. Scarisbrick.Isobel@Mayo.edu
Blaber
S I
SI
Tingling
J T
JT
Rodriguez
M
M
Blaber
M
M
Christophi
G P
GP
D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism; D001921:Brain / Q000276:immunology / Q000378:metabolism; D020278:Demyelinating Autoimmune Diseases, CNS / Q000276:immunology* / Q000378:metabolism*; D006728:Hormones / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013116:Spinal Cord / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013256:Steroids / Q000494:pharmacology; D013950:Thymus Gland / Q000276:immunology / Q000378:metabolism; D020840:Tissue Kallikreins / Q000096:biosynthesis* / Q000187:drug effects / Q000276:immunology
D006728:Hormones; D012333:RNA, Messenger; D013256:Steroids; D020840:Tissue Kallikreins