Speeding up three-dimensional protein NMR experiments to a few minutes.
We demonstrate for different protein samples that three-dimensional HNCO and HNCA correlation spectra may be recorded in a few minutes acquisition time using the band-selective excitation short-transient sequences presented here. This opens new perspectives for the NMR structural investigation of unstable protein samples and real-time site-resolved studies of protein kinetics.
Schanda
Paul
P
Institut de Biologie Structurale Jean-Pierre Ebel CNRS-CEA-UJF, 41 rue Jules Horowitz, 38027 Grenoble, France.
Van Melckebeke
Hélène
H
Brutscher
Bernhard
B
D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D013997:Time Factors