title: The surface display of haemolysin from Vibrio harveyi on yeast cells and their potential applications as live vaccine in marine fish.
abstract: HL1 gene encoding haemolysin from Vibrio harveyi SF-1 was expressed in yeast cells and the expressed haemolysin was displayed on the cell surface. After induction for 36h in galactose-containing medium, one-third of the cells contained the displayed protein and the displayed cells had haemolytic activity on erythrocytes from flounder. The double diffusion agar analysis showed that the sera from the flounder immunized with the displayed yeast cells having the haemolytic activity could form precipitate with the purified haemolysin. ELISA analysis indicated that immunization times had great influence on increased production of the specific antibody against haemolysin in turbot immunized with the displayed yeast cells having the haemolytic activity. After the challenge with V. harveyi SF-1, it was found that earlier protection in flounder and significant protection in turbot, both of which were immunized with the displayed yeast cells having the haemolytic activity, were achieved. These results suggested that the displayed yeast cells with the haemolytic activity could be used as potential live vaccine in marine fish.
authors.affiliation: UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong 266003, China.
authors.forename: Kailing
authors.initials: K
authors.lastname: Zhu
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Chi
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Fengli
authors.initials: F
authors.lastname: Zhang
authors.forename: Meiju
authors.initials: M
authors.lastname: Li
authors.forename: Hirimuthugoda Nalini
authors.initials: HN
authors.lastname: Yasoda
authors.forename: Longfei
authors.initials: L
authors.lastname: Wu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D004926:Escherichia coli / Q000378:metabolism; D005412:Flatfishes / Q000276:immunology*; D005818:Genetic Engineering; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D014733:Vibrio / Q000235:genetics / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology* / Q000401:mortality
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D006460:Hemolysin Proteins