title: ENA Actimineral Resource A restores bone loss and bone quality in ovariectomized rats.
abstract: The purpose of this study was to examine the effects of ENA Actimineral Resource A (ENA-A), seaweed origin alkaline water, on postmenopausal osteoporosis in ovariectomized (OVX) rats. The 12-week old Wistar rats were divided randomly into 4 groups: ovariectomized (OVX), OVX plus 0.5% ENA-A, OVX plus 5% ENA-A and OVX plus 10% ENA-A. A histopathological analysis indicated that ENA-A could prevent OVX-induced bone loss by increasing femur trabecular bone area in a dose-dependent manner. ENA-A significantly (p<0.05) increased serum estradiol levels, decreased serum osteocalcin activity and suppressed serum pyridinoline (PYD) levels. The in vitro effects of ENA-A were also studied using MC3T3-E1 cells. ENA-A significantly stimulated cell proliferation and increased both ALP activity and calcium deposition in a dose-dependent manner. These results suggest that the treatment of ovariectomized rats with ENA-A not only prevents bone resorption but also appears to maintain the cancellous bone structure of postmenopausal osteoporosis.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, #1370 Sangyeok-dong, Buk-gu, Daegu, 702-701, Republic of Korea.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Do
authors.forename: Sung-Yong
authors.initials: SY
authors.lastname: Hwa
authors.forename: Won-Il
authors.initials: WI
authors.lastname: Jeong
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: In-Seon
authors.initials: IS
authors.lastname: Lee
authors.forename: Kyu-Shik
authors.initials: KS
authors.lastname: Jeong
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000596:Amino Acids / Q000097:blood; D000818:Animals; D001835:Body Weight; D001862:Bone Resorption / Q000628:therapy*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D049109:Cell Proliferation; D004958:Estradiol / Q000097:blood; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D008900:Mineral Waters / Q000627:therapeutic use*; D008903:Minerals / Q000627:therapeutic use*; D015675:Osteocalcin / Q000097:blood; D010010:Osteoclasts / Q000166:cytology; D010012:Osteogenesis; D010052:Ovariectomy; D028321:Plant Preparations / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids; C518516:ENA Actimineral Resource A; D008900:Mineral Waters; D008903:Minerals; D028321:Plant Preparations; D012333:RNA, Messenger; D015675:Osteocalcin; D004958:Estradiol; C015484:pyridinoline; D000469:Alkaline Phosphatase