title: Melatonin prevents X-ray irradiation induced oxidative damagein peripheral blood and spleen of the seasonally breeding rodent, Funambulus pennanti during reproductively active phase.
abstract: OBJECTIVE
  Biological effects of X-ray irradiation and protection by melatonin on the immune status of a tropical rodent, Funambulus pennanti, was examined by estimating oxidative damage of peripheral blood and spleen and protection by melatonin treatment.
  
  
  METHODS
  Seventy squirrels were divided into 4 sets of 25, 25, 10 and 10 having subdivisions into a total of eight groups. Squirrels of set 1 (groups A and B) received only normal saline, set 2 (groups C and D) received 25 microg/100 g body weight (bwt) melatonin and set 3 (groups E and F) received 25 microg/100 g bwt vitamin E for four weeks. Groups G and H of set 4 received high doses of melatonin (0.5 mg/100 g bwt) 30 min prior to and 30 min after X-ray irradiation, respectively, and were sacrificed 1 h after irradiation. Groups B and D were sacrificed after 4 h, 24 h, 48 h and 72 h of irradiation for total leukocyte count (TLC) in peripheral blood, percent apoptotic cells and lipid peroxidation (LPO) in spleen while group F was sacrificed after 4 h of irradiation to measure LPO.
  
  
  RESULTS
  Pre-melatonin treatment (25 microg/100 g bwt) restored TLC, percent apoptotic cells and LPO levels of X-ray exposed squirrels. High dose pre-melatonin treatment (0.5 mg/100 g bwt) restored the above conditions significantly while post treatment did not. Vitamin E reduced elevated LPO level in irradiated tissue, but the effect of melatonin was more potent.
  
  
  CONCLUSIONS
  Melatonin administration prior to X-ray irradiation prevented radiation-induced oxidative damage during the reproductively active phase of the seasonally breeding rodent suggesting a high protective role of melatonin following X-ray irradiation.
authors.affiliation: Pineal Research Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Sharma
authors.forename: Chandana
authors.initials: C
authors.lastname: Haldar
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D015415:Biomarkers / Q000097:blood; D001835:Body Weight / Q000187:drug effects* / Q000528:radiation effects; D007958:Leukocyte Count; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000528:radiation effects; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000528:radiation effects; D008550:Melatonin / Q000008:administration & dosage / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects; D012098:Reproduction / Q000187:drug effects / Q000528:radiation effects; D012377:Rodentia; D012621:Seasons; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D013997:Time Factors; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology; D014965:X-Rays
chemical_list: D015415:Biomarkers; D014810:Vitamin E; D012965:Sodium Chloride; D008550:Melatonin