title: Binding characteristics of the Lactobacillus brevis ATCC 8287 surface layer to extracellular matrix proteins.
abstract: Self-assembling proteins that form crystalline surface layers on many microorganisms can be involved in bacterial-host adhesion via specific interactions with components of the extracellular matrix. Here, we describe the interaction of the Lactobacillus brevis ATCC 8287 surface-layer protein SlpA with fibronectin, laminin, fibrinogen and collagen using surface plasmon resonance. SlpA was found to interact with high affinity to fibronectin and laminin, with a respective binding constant of 89.8 and 26.7 nM. The interaction of SlpA with collagen and fibrinogen was found to be of much lower affinity, with respective binding constants of 31.8 and 26.1 microM. The serine protease inhibitor benzamidine greatly reduced the affinity of SlpA for fibronectin, whereas the affinity for laminin remained unaffected. No protease activity of the purified SlpA protein could be detected. These data suggest that L. brevis may interact with host cells directly through high affinity interactions with laminin and fibronectin predominantly, involving distinct regions of the SlpA protein.
authors.affiliation: Institute of Human Virology, University of Maryland Biotechnology Institute, Baltimore, MD 21201, USA. deleeuw@umbi.umd.edu
authors.forename: Erik
authors.initials: E
authors.lastname: de Leeuw
authors.forename: Xiangqun
authors.initials: X
authors.lastname: Li
authors.forename: Wuyuan
authors.initials: W
authors.lastname: Lu
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D052196:Levilactobacillus brevis / Q000378:metabolism*; D051379:Mice; D020349:Surface Plasmon Resonance
chemical_list: D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; C070683:surface layer protein A, Bacteria