title: A norbergenin derivative inhibits neuronal cell damage induced by tunicamycin.
abstract: Several chemically synthesized compounds were examined for protective effects against the cell damage in tunicamycin-treated human neuroblastoma IMR-32 cells. Among the compounds tested, an antioxidant, Norbergenin-11-caproate (10 microM), exhibited complete protection against the cell growth inhibitory effect of tunicamycin but did not inhibit the induction of Bip/GRP78 mRNA by tunicamycin. Both norbergenin-11-caproate and alpha-tocopherol completely inhibited the production of reactive oxygen species induced by tunicamycin, however, alpha-tocopherol inhibited tunicamycin-induced cell damage only partially, even at 100 microM. These findings suggest the potential of Norbergenin-11-caproate for therapeutic application in endoplasmic reticulum (ER) stress-dependent diseases implicating a specific mechanism other than anti-oxidative one.
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan. suzukis@ph.bunri-u.ac.jp
authors.forename: Shinya
authors.initials: S
authors.lastname: Suzuki
authors.forename: Yui
authors.initials: Y
authors.lastname: Okuse
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kawase
authors.forename: Masufumi
authors.initials: M
authors.lastname: Takiguchi
authors.forename: Yoshiyasu
authors.initials: Y
authors.lastname: Fukuyama
authors.forename: Hironobu
authors.initials: H
authors.lastname: Takahashi
authors.forename: Masao
authors.initials: M
authors.lastname: Sato
mesh_terms: D001578:Benzopyrans / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D017931:DNA Primers; D004365:Drugs, Chinese Herbal; D000091342:Endoplasmic Reticulum Chaperone BiP; D005434:Flow Cytometry; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007564:Japan; D018832:Molecular Chaperones / Q000235:genetics; D009447:Neuroblastoma; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014415:Tunicamycin / Q000633:toxicity*
chemical_list: D001578:Benzopyrans; D017931:DNA Primers; D004365:Drugs, Chinese Herbal; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D017382:Reactive Oxygen Species; D014415:Tunicamycin; C063922:norbergenin