Inhibitory effects of Agaricus blazei on mast cell-mediated anaphylaxis-like reactions.
Agaricus blazei is a medicinal mushroom native to Brazil. It used to be a source of anti-tumor and immunmoactive compounds and considered a health food in many countries. However, its specific effect against mast cell-mediated anaphylactic reactions is still unknown. In the present study, we investigated the effect of Agaricus blazei water extract (ABWE) on mast cell-mediated anaphylaxis-like reactions. We examined whether ABWE could inhibit systemic anaphylaxis-like reaction, ear swelling response, passive cutaneous anaphylaxis, and mast cell activation. ABWE inhibited compound 48/80-induced systemic anaphylaxis-like reaction, ear swelling response, and passive cutaneous anaphylaxis-like reaction in mice. ABWE also inhibited anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. ABWE dose-dependently inhibited compound 48/80-induced or anti-DNP IgE-mediated histamine release from rat peritoneal mast cells (RPMC). Moreover, pretreatment with ABWE reduced compound 48/80-induced calcium uptake into RPMC. When ABWE was added, the level of intracellular cAMP in RPMC showed a significant increase compared with that of control cells. In addition, ABWE significantly inhibited compound 48/80-induced cAMP reduction in RPMC. These results propose that ABWE may be beneficial in the treatment of mast cell-mediated anaphylactic reactions.
Choi
Yun Ho
YH
Department of Anatomy, Chonbuk National University Medical School, Jeonbuk, Republic of Kora.
Yan
Guang Hai
GH
Chai
Ok Hee
OH
Choi
Yung Hyun
YH
Zhang
Xin
X
Lim
Jung Min
JM
Kim
Ji-Hyun
JH
Lee
Moo Sam
MS
Han
Eui-Hyeog
EH
Kim
Hyoung Tae
HT
Song
Chang Ho
CH
D000364:Agaricus; D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004423:Ear; D004487:Edema / Q000517:prevention & control; D006636:Histamine Release / Q000187:drug effects; D007073:Immunoglobulin E / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D003189:p-Methoxy-N-methylphenethylamine
D010936:Plant Extracts; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D002118:Calcium