title: High-speed electroseparations inside silica colloidal crystals.
abstract: Crystals made from 200 nm silica colloids are hardened and chemically modified with chlorodimethyloctadecylsilane for use in electrically driven, reversed-phase separations. A van Deemter plot reveals extremely narrow peak widths for the separation of a cationic hydrophobic dye, DiI, with both the A and C terms 10-fold smaller than those for a conventional HPLC column. Electrically driven separations are demonstrated to be achieved in less than 10 s for three dyes differing in hydrophobicity and also for three peptides differing in electrophoretic mobility. The results show that these media are promising for high-speed separations.
authors.affiliation: Department of Chemistry, University of Arizona, 1306 East University Boulevard, Tucson, Arizona 85721, USA.
authors.forename: Suping
authors.initials: S
authors.lastname: Zheng
authors.forename: Eric
authors.initials: E
authors.lastname: Ross
authors.forename: Michael A
authors.initials: MA
authors.lastname: Legg
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Wirth
mesh_terms: D003102:Colloids / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D003102:Colloids; D012822:Silicon Dioxide