title: Comparative glycomics of the glycoprotein follicle stimulating hormone: glycopeptide analysis of isolates from two mammalian species.
abstract: Follicle stimulating hormone (FSH) is one of the important hormones that regulate gonadal functions. This hormone is glycosylated, and the glycans greatly influence the biological properties. In the present study the negatively charged glycopeptides of equine and human pituitary follicle stimulating hormone (eFSH and hFSH) have been characterized in a glycosylation site-specific manner using FT-ICR-MS and Edman sequencing. The characteristic pattern of glycan distribution at each glycosylation site has been deduced and compared between horse and human FSH preparations. The data suggest that site-specific differences exist between glycoforms of human and equine FSH. For instance, except for one site in the beta subunit (Asn7) of hFSH all other sites in both species have sulfated glycoforms. Also, glycoforms at Asn52 of hFSH are all complex type, whereas in eFSH, both complex and hybrid structures exist at this site. There is also a higher percentage of sulfated glycans in the latter site compared to the former. This is the first study that characterizes the glycans from this hormone in a glycosylation site-specific manner, and these data can be used to begin correlative studies between glycosylation structure and hormone function.
authors.affiliation: Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, USA.
authors.forename: Dilusha S
authors.initials: DS
authors.lastname: Dalpathado
authors.forename: Janet
authors.initials: J
authors.lastname: Irungu
authors.forename: Eden P
authors.initials: EP
authors.lastname: Go
authors.forename: Vladimir Y
authors.initials: VY
authors.lastname: Butnev
authors.forename: Katie
authors.initials: K
authors.lastname: Norton
authors.forename: George R
authors.initials: GR
authors.lastname: Bousfield
authors.forename: Heather
authors.initials: H
authors.lastname: Desaire
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005640:Follicle Stimulating Hormone / Q000737:chemistry*; D037201:Follicle Stimulating Hormone, beta Subunit / Q000737:chemistry*; D005583:Fourier Analysis; D006020:Glycopeptides / Q000737:chemistry; D015292:Glycoprotein Hormones, alpha Subunit / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry; D006031:Glycosylation; D006736:Horses; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis
chemical_list: D037201:Follicle Stimulating Hormone, beta Subunit; D006020:Glycopeptides; D015292:Glycoprotein Hormones, alpha Subunit; D006023:Glycoproteins; D011134:Polysaccharides; D005640:Follicle Stimulating Hormone