title: [Extraction and determination of short-chain fatty acids in biological samples].
abstract: Short-chain fatty acids are organic acids with 1 - 6 carbon atoms. Their physiological functions and clinical applications have attracted considerable attention. Their high polarity, low ultraviolet (UV) absorbance, low contents in biological samples, high volatility, good water-solubility and easy adsorption on metal and glass surfaces result in some difficulties in their separation and determination. This review focuses on the methods for extraction of short-chain fatty acids in biological samples such as feces, urine, blood and culture solutions by distillation, high speed centrifugation, liquid-solid extraction, solid phase microextration, supercritical fluid extraction and liquid phase microextraction and determination by gas chromatography, high performance liquid chromatography and capillary electrophoresis. Sixty-three references are cited.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science, Education Ministry of China, Nanjing University, Nanjing 210093, China. Tanliu@sohu.com
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
mesh_terms: D000327:Adsorption; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D057168:Distillation; D019075:Electrophoresis, Capillary / Q000379:methods; D005232:Fatty Acids, Volatile / Q000032:analysis / Q000302:isolation & purification*; D005243:Feces / Q000737:chemistry; D008872:Microwaves; D009930:Organic Chemicals / Q000302:isolation & purification; D024301:Plant Bark / Q000737:chemistry*; D052617:Solid Phase Microextraction; D013048:Specimen Handling / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005232:Fatty Acids, Volatile; D009930:Organic Chemicals