[Determination of nucleic acids using toluidine blue as a fluorescence probe].
A new fluorimetric method has been developed for the determination of deoxyribonucleic acid (DNA) with toluidine blue (TB) as a fluorescence probe. It is based on the fluorescence quenching of toluidine blue in the presence of DNA. In Tris-HCl buffer solution (pH 8.5), the calibration graph was linear over the range 0.10-6.00 microg x mL(-1) for ctDNA, and the detection limit was 27 ng x mL(-1). The result of the determination of DNA of camphor tree's leaf by this method was satisfactory.
Guo
Liang-qia
LQ
Department of Chemistry, Fuzhou University, Fuzhou 350002, China.
Ye
Fang-gui
FG
Lin
Xu-cong
XC
Xie
Zeng-hong
ZH
D004247:DNA / Q000032:analysis*; D018744:DNA, Plant / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014048:Tolonium Chloride / Q000032:analysis*
D018744:DNA, Plant; D005456:Fluorescent Dyes; D014048:Tolonium Chloride; D004247:DNA; C427203:calf thymus DNA