[Effect of gingerol on endotoxemia mouse model induced by heatstroke].
OBJECTIVE
To observe the effect of Gingerol on endotoxemia mouse induced by heatstroke.


METHODS
Forty mice were randomly divided into five groups, the endotoxemia model group (A), the normal temperature group (B), the Gingerol treated group (C), the solvent control group (D), and the saline control group (E), 8 mice in each group. Group B to E was administered with saline, Gingerol, solvent and saline respectively. Mice in group B were placed at room temperature 25 +/- 0.5 degrees C , relative humidity 43 +/- 5 % for 2 hrs, while mice in the other groups were exposed under 35 +/- 0.5 degrees C and relative humidity 65 +/- 5 % for 2 hrs in an artificial hot-climate mimic cabin to establish heatstroke endotoxemia model. The energy metabolic level of celiomacrophage was detected with MTT; the phagocytic ability was examined with neutral red chromometry; the hepatocyte ultrastructure was observed with transmission electron microscopy, as well as the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) in plasma was tested.


RESULTS
As compared with Group A, D and E, in Group C, energy metabolic levels of macrophage, phagocytic ability, and activity of SOD were significantly higher (P < 0.01), and the level of MDA was significantly lower respectively (P < 0.01), with the levels of SOD and MDA approaching to those in Group B (P >0.05). The pathologic changes of hepatocyte ultrastructure in group C were less than those in the other three endotoxemia groups.


CONCLUSIONS
Gingerol could raise the energy metabolic level of celio-macrophage to enhance its phagocytic ability, increase the activity of SOD and reduce the production of MDA in mouse with heatstroke endotoxemia, so as to alleviate the liver damage.
Nie
Hong
H
Department of Pharmacology, College of Pharmacy, Jinan University, Guangzhou. tnieh@jnu.edu.cn
Meng
Lan-zhen
LZ
Zhang
Hui
H
D000818:Animals; D002396:Catechols; D019446:Endotoxemia / Q000188:drug therapy* / Q000209:etiology; D005233:Fatty Alcohols / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D020939:Zingiber officinale / Q000737:chemistry; D018883:Heat Stroke / Q000150:complications; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D008517:Phytotherapy; D011897:Random Allocation
D002396:Catechols; D005233:Fatty Alcohols; C007845:gingerol