title: [Efficacy of HSV-tk/GCV system on human laryngeal squamous cell cancer in vitro].
abstract: OBJECTIVE
  Efficacy of HSV-tk/GCV system antitumor effects was assessed on human laryngeal cancer cell line Hep-2 in vitro. To assess the HSV-tk/CGV system whether has an antitumour effect on human laryngeal squamous cell cancer Hep-2 in vitro. The mechanisms of cytotoxity were also assessed.
  
  
  METHODS
  Hep-2 cells were transfected with HSV-tk gene by lipofection. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the HSV-tk gene expression. MTT was utilized to test for the cytotoxicity of this system. The cell-circle arrest and apoptosis were analyzed by flowcytometry assay.
  
  
  RESULTS
  HSV-tk gene transfected cells demonstrated obvious cytoreductivity followed by ganciclovir (GCV) administration and this cytoreductivity showed partial GCV dose-independent. HSV-tk gene transfected cells demonstrated obvious s-phase arrest, no apoptosis and necrosis occurred.
  
  
  CONCLUSIONS
  The HSV-tk/GCV system can inhabit the growth of Hep-2 cells effectively. S-phase arrest perhaps is the main reason that leads to the cell inhibition in our study. HSV-tk/GCV system has potential antitumor effects for the future clinical practice.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery of Peking University, the First Hospital, Beijing 100034, China.
authors.forename: Xiu-yong
authors.initials: XY
authors.lastname: Ding
authors.forename: Yong
authors.initials: Y
authors.lastname: Qin
authors.forename: Fu-ying
authors.initials: FY
authors.lastname: Li
authors.forename: Tie-chuan
authors.initials: TC
authors.lastname: Cong
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000628:therapy*; D045744:Cell Line, Tumor; D015774:Ganciclovir; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D007822:Laryngeal Neoplasms / Q000628:therapy*; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection
chemical_list: D013937:Thymidine Kinase; D015774:Ganciclovir