Stimulation of pigment accumulation in Anabaena azollae strains: effect of light intensity and sugars.
The influence of high light intensity on the growth and pigment accumulating ability of Anabaena azollae was investigated. A. azollae responded positively to high light intensity (6 klx) and was further evaluated at higher intensities (10 and 15 klx), in the presence of glucose, sucrose and jaggery +/- DCMU. Significant enhancement in phycobiliproteins and carotenoids was observed in the sugar supplemented cultures at high light intensities. SDS-PAGE profiles of whole cell proteins revealed the presence of unique bands in such treatments. Sucrose supplementation induced a 30-90 % increase in carotenoids, phycocyanin and phycoerythrin content at 10 klx. Molecular analysis of the stimulatory and interactive role of sugars on pigment enhancement at high light intensity may aid in better exploitation of cyanobacteria as a source of pigments.
Venugopal
V
V
Center for Conservation and Utilization of Blue-Green Algae, Indian Agricultural Research Institute, New Delhi.
Prasanna
R
R
Sood
A
A
Jaiswal
P
P
Kaushik
B D
BD
D017033:Anabaena / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D050260:Carbohydrate Metabolism; D002338:Carotenoids / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000032:analysis; D010798:Phycocyanin / Q000032:analysis; D010799:Phycoerythrin / Q000032:analysis; D010860:Pigments, Biological / Q000032:analysis*; D010936:Plant Extracts / Q000378:metabolism; D020543:Proteome / Q000032:analysis; D031786:Saccharum / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D001426:Bacterial Proteins; C060188:Gur; D045342:Light-Harvesting Protein Complexes; D010860:Pigments, Biological; D010936:Plant Extracts; D020543:Proteome; D010798:Phycocyanin; D010799:Phycoerythrin; D002338:Carotenoids; D013395:Sucrose; D005947:Glucose