Growth inhibition of stilbenoids in Welwitschiaceae and Gnetaceae through induction of apoptosis in human leukemia HL60 cells.
Fifty-six stilbenoids isolated from the families of Welwitschiaceae and Gnetaceae were screened for growth inhibitory activity against HL60 cells, and two compounds (gnemonol G and gnetin I) among them exhibited a strong activity with IC(50) of 10.0 microM and 12.2 microM at 48 h incubation, respectively. The growth suppression by gnemonol G and gnetin I was found to be in part due to apoptosis which was assessed by morphological findings such as nuclear condensation and fragmentation, and DNA ladder formation in human leukemia HL60 cells.
Iliya
Ibrahim
I
Gifu Pharmaceutical University, Japan.
Akao
Yukihiro
Y
Matsumoto
Kenji
K
Nakagawa
Yoshihito
Y
Zulfiqar
Ali
A
Ito
Tetsuro
T
Oyama
Masayoshi
M
Murata
Hiroko
H
Tanaka
Toshiyuki
T
Nozawa
Yoshinori
Y
Iinuma
Munekazu
M
D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects / Q000235:genetics; D029624:Ferns; D018922:HL-60 Cells; D006801:Humans; D007214:Indonesia; D007700:Kinetics; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D018547:Plant Stems; D013267:Stilbenes / Q000494:pharmacology*
D004273:DNA, Neoplasm; D010936:Plant Extracts; D013267:Stilbenes