title: A double mutant of Pseudomonas putida JLR11 deficient in the synthesis of the nitroreductase PnrA and assimilatory nitrite reductase NasB is impaired for growth on 2,4,6-trinitrotoluene (TNT).
abstract: Pseudomonas putida JLR11 can grow on 2,4,6-trinitrotoluene (TNT) as the sole nitrogen source. We created nasB (nitrite reductase), pnrA (nitroaromatic reductase) and pnrA nasB mutants and tested their growth with TNT as the sole N source. The nasB and pnrA mutants grew at a reduced rate on TNT, whereas the double nasB pnrA mutant did not. This suggests that P. putida JLR11 carries out multiple enzymatic attacks on TNT-releasing nitrite and/or ammonium. The PnrA nitroreductase plays a key role in the reduction of TNT to 2,6-dinitro-4-hydroxylaminotoluene and the subsequent release of ammonium for growth.
authors.affiliation: Estación Experimental del Zaidin, Department of Biochemistry, Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Caballero
authors.forename: Juan L
authors.initials: JL
authors.lastname: Ramos
mesh_terms: D009572:Nitrite Reductases / Q000172:deficiency* / Q000235:genetics; D009601:Nitroreductases / Q000172:deficiency* / Q000235:genetics; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014303:Trinitrotoluene / Q000378:metabolism*
chemical_list: D014303:Trinitrotoluene; D009572:Nitrite Reductases; D009601:Nitroreductases