Incorporating water-release and lateral protein interactions in modeling equilibrium adsorption for ion-exchange chromatography.
The equilibrium adsorption of two albumin proteins on a commercial ion exchanger has been studied using a colloidal model. The model accounts for electrostatic and van der Waals forces between proteins and the ion exchanger surface, the energy of interaction between adsorbed proteins, and the contribution of entropy from water-release accompanying protein adsorption. Protein-surface interactions were calculated using methods previously reported in the literature. Lateral interactions between adsorbed proteins were experimentally measured with microcalorimetry. Water-release was estimated by applying the preferential interaction approach to chromatographic retention data. The adsorption of ovalbumin and bovine serum albumin on an anion exchanger at solution pH>pI of protein was measured. The experimental isotherms have been modeled from the linear region to saturation, and the influence of three modulating alkali chlorides on capacity has been evaluated. The heat of adsorption is endothermic for all cases studied, despite the fact that the net charge on the protein is opposite that of the adsorbing surface. Strong repulsive forces between adsorbed proteins underlie the endothermic heat of adsorption, and these forces intensify with protein loading. It was found that the driving force for adsorption is the entropy increase due to the release of water from the protein and adsorbent surfaces. It is shown that the colloidal model predicts protein adsorption capacity in both the linear and non-linear isotherm regions, and can account for the effects of modulating salt.
Thrash
Marvin E
ME
Department of Chemical and Materials Engineering, University of Cincinnati, Cincinnati, OH 45221-0012, USA.
Pinto
Neville G
NG
D000327:Adsorption; D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods*; D019277:Entropy; D010047:Ovalbumin / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*
D012710:Serum Albumin, Bovine; D010047:Ovalbumin