title: Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococcus fascians.
abstract: In a search for novel immunostimulating substances we detected that culture supernatants of the gram-positive phytopathogenic bacterium, Rhodococcus fascians, were able to induce cytokine release (TNF(alpha)) from mouse peritoneal macrophages. Monoclonal antibodies were generated against the active principle, and were employed for its isolation and partial characterization as a high molecular (MW>100 kDa) glycoprotein. In addition, methods practicable for its biotechnological preparation and several ELISA variants for its determination were developed.
authors.affiliation: Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany. uwe.karsten@glycotope.com
authors.forename: Günter
authors.initials: G
authors.lastname: Butschak
authors.forename: Uwe
authors.initials: U
authors.lastname: Karsten
authors.forename: Ute
authors.initials: U
authors.lastname: Schelhaas
authors.forename: Holger
authors.initials: H
authors.lastname: Ott
authors.forename: Andreas
authors.initials: A
authors.lastname: Emmendörffer
authors.forename: Bernd
authors.initials: B
authors.lastname: Niemeyer
authors.forename: Heike
authors.initials: H
authors.lastname: Helmholz
authors.forename: Steffen
authors.initials: S
authors.lastname: Goletz
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D007155:Immunologic Factors / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D051379:Mice; D012240:Rhodococcus / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006023:Glycoproteins; D007155:Immunologic Factors