[Supercritical fluid extraction of paeonol from Liuwei Dihuang pill].
OBJECTIVE
To study the method of extraction of active ingredient from complex Chinese patent medicine.


METHODS
Supercritical fluid extraction (SFE) has been used to extract paeonol from Liuwei Dihuang pill and HPLC was used to analyze the extracts.


RESULTS
The methanol modified supercritical CO2 (0.4 mL x min(-1)) can effectively extract paeonol from Liuwei Dihuang pill.


CONCLUSIONS
SFE-HPLC method of off-line for analysis of extract was simple, quick and accurate.
Chang
Yi-ling
YL
Zhejiang Pharmaceutical College, Ningbo, China.
Liu
Ben
B
D000098:Acetophenones / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D013607:Tablets
D000098:Acetophenones; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D013607:Tablets; C476907:liu-wei-di-huang wan; C013638:paeonol