Modulation of the specific allergic response by mite allergens encapsulated into liposomes.
Liposomes are non toxic and biodegradable lipid vesicles, which are safe and effective adjuvants to induce Th1-skewed immune response. Therefore, the encapsulation of allergens into liposomes could be an attractive alternative for specific allergy immunotherapy. Previously, we obtained DPPC iposomes encapsulating purified allergens from Dermatophagoides siboney, with suitable stability and extremely reduced allergenicity. In this study, Balb/c mice were immunized with allergens ncapsulated into liposomes (LP) and the induced immune response was evaluated in comparison with allergens dissolved in PBS (PBSA) or adsorbed in Alum (AL). The use of Alum or Liposomes induced a strong allergen specific IgG response. However, total IgE serum levels in the AL group were very high, while levels found in LP group were not significantly different from the control group receiving only PBS. The IgG2a/IgG1 subclass ratio was raised in the LP group. Allergen specific IgE, as measured by PCA assay, was similar for LP and PBSA groups, and approximately the half of the reaction size found in AL group. After allergen challenge by inhalation route, peripheral blood and airway eosinophil counts increased significantly in AL, but not in LP group. Additionally, histopathological analysis of lung tissue sections obtained from challenged mice indicated a reduced cellular infiltration in mice immunized with liposomes. These results support the potential use of liposomal formulations for allergen vaccines.
Calderón
Lesly
L
Centro de Estudio de Proteínas, Fac. Biología, U.H., C. Habana, Cuba. leslycd@fbio.uh.cu
Facenda
Elisa
E
Machado
Liuber
L
Uyema
Keiko
K
Rodríguez
Dunia
D
Gomez
Eliane
E
Martínez
Yohanka
Y
González
Bárbara
B
Bourg
Virgilio
V
Alvarez
Carlos
C
Otero
Anselmo
A
Russo
Momtchilo
M
Labrada
Alexis
A
Lanio
María E
ME
D000534:Alum Compounds; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage / Q000276:immunology*; D006967:Hypersensitivity / Q000276:immunology*; D008081:Liposomes / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C
D000534:Alum Compounds; D039741:Antigens, Dermatophagoides; D008081:Liposomes; C041524:aluminum sulfate