title: Characterization of two new rhabdoviruses isolated from midges (Culicoides SPP) in the Brazilian Amazon: proposed members of a new genus, Bracorhabdovirus.
abstract: Itacaiunas and Curionopolis viruses were isolated from Culicoides midges in Parauapebas municipality, Pará state, Brazil, in 1984 and 1985, respectively. Itacaiunas virus infected newborn mice and mosquito cells (C6/36), but did not replicate in some mammalian cell lineages; while Curionopolis virus infected only mice. Neither virus showed a serological relationship with any of the 195 known arboviruses circulating in Brazil, nor against 38 other rhabdoviruses isolated worldwide. Both virus particles are bullet-shaped and similar in morphology to that observed for other members of the family Rhabdoviridae. Partial nucleotide sequencing of the N protein showed that those two viruses constitute a separate clade in the family Rhabdoviridae, which we propose to be a new genus, designated Bracorhabdovirus.
authors.affiliation: Seção de Microscopia Eletrônica, Instituto Evandro Chagas, SVS, Ministério da Saúde, Belém, Brazil.
authors.forename: J A P
authors.initials: JA
authors.lastname: Diniz
authors.forename: M R T
authors.initials: MR
authors.lastname: Nunes
authors.forename: A P A
authors.initials: AP
authors.lastname: Travassos da Rosa
authors.forename: A C R
authors.initials: AC
authors.lastname: Cruz
authors.forename: W
authors.initials: W
authors.lastname: de Souza
authors.forename: D B A
authors.initials: DB
authors.lastname: Medeiros
authors.forename: J O
authors.initials: JO
authors.lastname: Chiang
authors.forename: P F C
authors.initials: PF
authors.lastname: Vasconcelos
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000648:ultrastructure / Q000821:virology; D001938:Brazil; D002519:Ceratopogonidae / Q000821:virology*; D051379:Mice; D008854:Microscopy, Electron; D012209:Rhabdoviridae / Q000145:classification* / Q000302:isolation & purification / Q000648:ultrastructure