Polymer-coated hollow fiber microextraction combined with on-column stacking in capillary electrophoresis.
In this work, a novel microextraction method termed polymer-coated hollow fiber microextraction (PC-HFME) was developed in combination with capillary electrophoresis (CE). Polar dihydroxylated polymethylmethacrylate polymer was coated onto a porous propylene hollow fiber membrane and used as an adsorbent and that was placed in a stirred aqueous sample solution. Tumbling of the extraction device within the sample solution facilitated extraction. The amino alcohols (2-amino-1-phenylethanol, norephedrine, alprenolol and atenolol which are beta-blocker drugs), were used as model compounds to investigate the extraction performance. No organic solvent was used in this procedure. The extract was then further concentrated through on-column stacking (normal stacking mode) during CE analysis. The detection limits ranged from 0.9 to 7 ng ml(-1). Relative standard deviations (n=6) ranged from 4 to 6%. The extraction of the amino alcohols in spiked wastewater effluent (representing a complex matrix) was evaluated using the developed procedure.
Basheer
Chanbasha
C
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
Wang
Huijuan
H
Jayaraman
Akhila
A
Valiyaveettil
Suresh
S
Lee
Hian Kee
HK
D000605:Amino Alcohols / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis
D000605:Amino Alcohols; D014874:Water Pollutants, Chemical; D012965:Sodium Chloride; D019904:Polymethyl Methacrylate