Cryopreservation of hematopoietic and non-hematopoietic stem cells.
Recent studies illustrate the potential for improving the cryopreservation of stem cells. Reduced DMSO concentrations in the cryopreservation medium, post thaw washing of cells and increased cell concentration have been actively studied. Standardization of cell processing has led to the study of liquid storage prior to cryopreservation, validation of mechanical (uncontrolled rate freezing) freezing, and cryopreservation bag failure. Finally, the need for the systematic study and optimization of preservation processes has not been fulfilled. As the sources and applications of stem cells (hematopoietic and non-hematopoietic) continue to be developed, the need for effective preservation methods will only grow.
Fleming
K K
KK
Department of Mechanical Engineering, University of Minnesota, 1100 Mechanical Engineering, 111 Church Street SE, Minneapolis, MN 55455, USA.
Hubel
A
A
D015925:Cryopreservation / Q000379:methods*; D004121:Dimethyl Sulfoxide; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006413:Hematopoietic System / Q000166:cytology; D006801:Humans; D008648:Mesoderm
D004121:Dimethyl Sulfoxide