The dynamic developmental localization of the full-length corticotropin-releasing factor receptor type 2 in rat cerebellum.
Corticotropin releasing factor receptor 2 (CRF-R2) is strongly expressed in the cerebellum and plays an important role in the development of the cerebellar circuitry, particularly in the development of the dendritic trees and afferent input to Purkinje cells. However, the mechanisms responsible for the distribution and stabilization of CRF-R2 in the cerebellum are not well understood. Here, we provide the first detailed analysis of the cellular localization of the full-length form of CRF-R2 in rat cerebellum during early postnatal development. We document unique and developmentally regulated subcellular distributions of CRF-R2 in cerebellar cell types, e.g. granule cells after postnatal day 15. The presence of one or both receptor isoforms in the same cell may provide a molecular basis for distinct developmental processes. The full-length form of CRF-R2 may be involved in the regulation of the first stage of dendritic growth and at later stages in the controlling of the structural arrangement of immature cerebellar circuits and in the autoregulatory pathway of the cerebellum.
Gounko
Natalia V
NV
Department of Cell Biology, Laboratory for Electron Microscopy, University Medical Center Groningen, University of Groningen, 9713 AV Groningen, The Netherlands. n.v.gounko@med.umcg.nl
Kalicharan
Dharamdajal
D
Rybakin
Vasily
V
Gramsbergen
Albert
A
van der Want
Johannes J L
JJ
D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development*; D008297:Male; D011689:Purkinje Cells / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D018019:Receptors, Corticotropin-Releasing Hormone / Q000032:analysis*
C101531:CRF receptor type 2; D018019:Receptors, Corticotropin-Releasing Hormone