title: Comparative analysis of two-dimensional electrophoresis maps (2-DE) of Helicobacter pylori from Brazilian patients with chronic gastritis and duodenal ulcer: a preliminary report.
abstract: Helicobacter pylori is a bacterium recognized as the major cause of peptic ulcer and chronic gastritis. Recently, a proteome-based approach was developed to investigate pathogenic factors related to H. pylori. In this preliminary study, H. pylori strains were isolated from gastric biopsies of patients with chronic gastritis and duodenal ulcers. A partial proteomic analysis of H. pylori strains was performed by bacterial lyses and proteins were separated by two-dimensional gel electrophoresis (2-DE). A comparative analysis was performed to verify a differential protein expression between these two 2-DE maps. These data should be useful to clarify the role of different proteins related to bacterial pathogenesis. This study will be completed using a larger number of samples and protein identification of H. pylori by MALDI-TOF mass spectrometry.
authors.affiliation: Laboratório de Química de Proteínas, Instituto de Biologia, Universidade Estadual de Campinas, Rua Carlos Chagas 420, 13081-970 Campinas, São Paulo, Brazil. diegomatao@yahoo.com.br
authors.forename: Diego R B
authors.initials: DR
authors.lastname: Pereira
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins
authors.forename: Flavia V
authors.initials: FV
authors.lastname: Winck
authors.forename: Marcus B
authors.initials: MB
authors.lastname: Smolka
authors.forename: Nancy F
authors.initials: NF
authors.lastname: Nishimura
authors.forename: Elizabeth M A
authors.initials: EM
authors.lastname: Rabelo-Gonçalves
authors.forename: Natalicia H
authors.initials: NH
authors.lastname: Hara
authors.forename: Sérgio
authors.initials: S
authors.lastname: Marangoni
authors.forename: José Murilo R
authors.initials: JM
authors.lastname: Zeitune
authors.forename: José Camillo
authors.initials: JC
authors.lastname: Novello
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002908:Chronic Disease; D004381:Duodenal Ulcer / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005753:Gastric Mucosa / Q000382:microbiology; D005756:Gastritis / Q000382:microbiology*; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000737:chemistry*; D006801:Humans; D020543:Proteome / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D020543:Proteome