title: [In vitro preservation of laryngeal allograft for larynx transplantation].
abstract: OBJECTIVE
  As a complex organ, laryngeal allograft cannot endure more than 3 hours during preservative time in vitro. This study was to explore the best preservation method and time limit of laryngeal allograft.
  
  
  METHODS
  During transplantation experiment of the larynx, HCA solution and UW's solution was used to perfuse laryngeal allograft on original position separately. The sequential morphologic changes of the allograft after cold preservation were observed to compare the effects of the 2 solutions.
  
  
  RESULTS
  After preservation in HCA solution for 20 h, ultrastructure changes, including mitochondrial edema or collapse and chromatin agglutination, appeared in mucosa, capillary and intrinsic laryngeal muscle cells of the laryngeal allograft; nuclear pyknosis was observed after 22 h and followed by apoptosis. While ischemic and necrosis changes of the laryngeal allograft appeared after preservation in UW's solution for 24 h.
  
  
  CONCLUSIONS
  UW's solution can preserve laryngeal allograft for 24 h in vitro, and has better effect than HCA solution.
authors.affiliation: State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510060, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Zhu-Ming
authors.initials: ZM
authors.lastname: Guo
authors.forename: Han-Wei
authors.initials: HW
authors.lastname: Peng
authors.forename: Fu-Jin
authors.initials: FJ
authors.lastname: Chen
authors.forename: Zong-Yuan
authors.initials: ZY
authors.lastname: Zeng
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D005260:Female; D005978:Glutathione; D007328:Insulin; D007830:Larynx / Q000637:transplantation*; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose