Development of a high performance liquid chromatographic method for systematic quantitative analysis of chemical constituents in rhubarb.
HPLC methods for the systematic determination of 30 compounds in Rhei Rhizoma (rhubarb) were developed. Using a combination of mobile phase gradient conditions and UV detection at 280 nm, all 30 compounds were separated satisfactorily with low detection limits (0.05-2 microg/ml). The developed methods provided a reliable calibration curve for each compound. By adopting these methods, the determination of 30 compounds in three kinds of rhubarb samples, derived from Rheum tanguticum, R. palmatum and R. officinale, was achieved. The constituent pattern of each rhubarb was clearly characterized through the quantitative composition of 30 major constituents of rhubarb.
Komatsu
Katsuko
K
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama; 2630 Sugitani, Toyoma 930-0194, Japan. katsukok@ms.toyama-mpu.ac.jp
Nagayama
Yorinobu
Y
Tanaka
Ken
K
Ling
Yun
Y
Basnet
Purusotam
P
Meselhy
Meselhy Ragab
MR
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D012250:Rheum / Q000737:chemistry*