Segregation of partly melted DNA molecules.
Segregation of partly melted DNA molecules is a convenient and efficient method to isolate DNA fragments associated with CpG islands. The method stands on the observation that the electrophoretic mobility of partly melted DNA fragments in a denaturing gradient gel is low and that they persist in the gel so long as the remaining helical part is sufficiently resistant to strand dissociation and dissociates slowly. Such features are observed in DNA fragments derived from CpG islands. These DNA fragments are preferentially retained in a denaturing gradient gel after prolonged electric field exposure, permitting the enrichment of DNA fragments derived from CpG islands. The principle and practical application of this method are reviewed.
Shiraishi
Masahiko
M
Department ofPharmaceutical Sciences, International University of Health and Welfare, Otawara, Tochigi, Japan. mshirais@iuhw.ac.jp
Sekiya
Takao
T
D020029:Base Pairing; D018899:CpG Islands / Q000235:genetics*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D019175:DNA Methylation; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D009691:Nucleic Acid Denaturation
D004247:DNA