T-cell trafficking competence is required for CNS invasion.
T-cell invasion of the CNS is critical for the induction of a variety of autoimmune mediated neuronal diseases. We utilized blood-brain barrier (BBB) mediated exclusion of anti-CD4 antibody to define populations of encephalitogenic T-cells recovered from mouse CNS preparations as either CNS invasive or non-invasive. This separation of cells allowed flow cytometric examination of the kinetics of encephalitogenic T-cell entry past the BBB. Further experiments examined the relative contribution of EAE inflammatory conditioning of the BBB to the kinetics of T-cell adherence and migration into the CNS. Inflammatory conditioning was found to have no effect on accumulation of T-cells at the vascular interface of the BBB, but was found to increase the entry of adoptively transferred T-cells into the CNS following their initial adherence to the BBB.
Lees
Jason R
JR
Department of Molecular Biology and Pharmacology, Campus Box 8103, 660 South Euclid, Washington University, St. Louis 63110, USA.
Archambault
Angela S
AS
Russell
John H
JH
D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D001812:Blood-Brain Barrier / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D002490:Central Nervous System / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D004195:Disease Models, Animal; D004660:Encephalitis / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D042783:Endothelial Cells / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D013601:T-Lymphocytes / Q000276:immunology*
D015704:CD4 Antigens