Antibrain antibodies in children with autism and their unaffected siblings.
Serum autoantibodies to human brain, identified by ELISA and Western immunoblotting, were evaluated in 29 children with autism spectrum disorder (22 with autistic disorder), 9 non-autistic siblings and 13 controls. More autistic subjects than controls had bands at 100 kDa in caudate, putamen and prefrontal cortex (p<0.01) as well as larger peak heights of bands at 73 kDa in the cerebellum and cingulate gyrus. Both autistic disorder subjects and their matched non-autistic siblings had denser bands (peak height and/or area under the curve) at 73 kDa in the cerebellum and cingulate gyrus than did controls (p<0.01). Results suggest that children with autistic disorder and their siblings exhibit differences compared to controls in autoimmune reactivity to specific epitopes located in distinct brain regions.
Singer
Harvey S
HS
Department of Neurology, Johns Hopkins University School of Medicine, Jefferson Street Building 124, 600 N. Wolfe Street, Baltimore, MD 21287-1000, USA. hsinger@jhmi.edu
Morris
Christina M
CM
Williams
Phillip N
PN
Yoon
Dustin Y
DY
Hong
John J
JJ
Zimmerman
Andrew W
AW
D001321:Autistic Disorder / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D001921:Brain / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D010375:Pedigree; D035781:Siblings
D001323:Autoantibodies