title: A new approach for DNA detection by SERRS.
abstract: A new approach for the detection of DNA using surface enhance resonance Raman scattering (SERRS) is reported. The majority of existing techniques use fluorescence spectroscopy with advanced probe design to provide information on the identity of specific DNA sequences down to single base resolution. A new approach to the labelling of DNA is discussed which uses Michael addition to couple thiolated DNA to dye labels specifically designed to attach to silver surfaces. When combined with existing strategies for sensitive detection of DNA using commercially available labels, a new class of biomolecular probe known as a SERRS Beacon was produced. The detection techniques of fluorescence and surface enhanced resonance Raman scattering (SERRS) are combined to give a sensitive and selective system for use in the development and creation of novel assays for specifically defined targets. It demonstrates improved potential for multiplexing analysis.
authors.affiliation: Duncan Graham, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UK. Karen.faulds@strath.ac.uk
authors.forename: Karen
authors.initials: K
authors.lastname: Faulds
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Fruk
authors.forename: David C
authors.initials: DC
authors.lastname: Robson
authors.forename: David G
authors.initials: DG
authors.lastname: Thompson
authors.forename: Alexis
authors.initials: A
authors.lastname: Enright
authors.forename: W Ewen
authors.initials: WE
authors.lastname: Smith
authors.forename: Duncan
authors.initials: D
authors.lastname: Graham
mesh_terms: D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D008301:Maleimides; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties; D014230:Triazoles
chemical_list: D015342:DNA Probes; D008301:Maleimides; D014230:Triazoles; C043592:maleimide; C012771:benzotriazole; D004247:DNA