Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins.
Despite major technical advance in methods used for structural investigations of proteins structure determination of membrane proteins still poses a significant challenge. Recently, the application of cell-free expression systems to membrane proteins has demonstrated that this technique can be used to produce quantities sufficient for structural investigations for many different membrane proteins. In particular for NMR spectroscopy, cell-free expression provides major advantages since it allows for amino acid type selective and even amino acid position specific labeling. In this mini-review we discuss the combination of cell-free membrane protein expression and liquid state NMR spectroscopy.
Koglin
Alexander
A
Institute for Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Marie-Curie Str. 9, 60439 Frankfurt, Germany.
Klammt
Christian
C
Trbovic
Nikola
N
Schwarz
Daniel
D
Schneider
Birgit
B
Schäfer
Birgit
B
Löhr
Frank
F
Bernhard
Frank
F
Dötsch
Volker
V
D002474:Cell-Free System / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017433:Protein Structure, Secondary; D012997:Solvents / Q000737:chemistry
D008565:Membrane Proteins; D012997:Solvents