Intracellular Ca2+ homeostasis and aggregation in platelets are impaired by ethanol through the generation of H2O2 and oxidation of sulphydryl groups.
The mechanisms involved in the effect of ethanol on Ca2+ entry and aggregability have been investigated in human platelets in order to shed new light on the pathogenesis of alcohol consumption. Ethanol (50 mM) induced H2O2 production in platelets by Ca2+-dependent and independent mechanisms. Ca2+ entry induced by ethanol was impaired by catalase. Ethanol reduced SOCE mediated by depletion of the 2,5-di-(tert-butyl)-1,4-hydroquinone (TBHQ)-sensitive acidic stores but enhances SOCE regulated by the dense tubular system. This effect was abolished by treatment with catalase or the sulphydryl group reducing agent dithiotreitol (DTT). Similarly, the anti-aggregant effect of ethanol was prevented by platelet treatment with catalase or DTT. In conclusion we provide considerable evidence that ethanol alters Ca2+ entry and reduces thrombin-induced aggregation as a result of the generation of H2O2 and the oxidation of sulphydryl groups in human platelets.
Rosado
Juan A
JA
Department of Physiology (Cellular Physiology Research Group), University of Extremadura, 10071 Cáceres, Spain.
Nuñez
Ana M
AM
Lopez
Jose J
JJ
Pariente
Jose A
JA
Salido
Gines M
GM
D002118:Calcium / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004229:Dithiothreitol / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D006706:Homeostasis / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D010974:Platelet Aggregation / Q000187:drug effects* / Q000502:physiology; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology; D013997:Time Factors
D013438:Sulfhydryl Compounds; D000431:Ethanol; D006861:Hydrogen Peroxide; D002374:Catalase; D013917:Thrombin; D002118:Calcium; D004229:Dithiothreitol