title: The role of B cells in the immune response to pestivirus (classical Swine Fever virus).
abstract: Pigs inoculated with the Alfort 187 isolate of classical swine fever (CSF) virus were used to study the immunological mechanisms associated with the humoral immune response in the disease. Quantitative and qualitative changes in the B-cell population (lambda light chain [C-lambda]-positive, immunoglobulins [Ig]-M-positive, and IgG-positive were demonstrated in the spleen, thymus and ileocaecal lymph node. Blood and serum samples were used to examine changes in leucocytes, albumin/globulin ratios and specific antibodies against CSF virus titration. Despite the lymphoid depletion shown by infected animals, an increase in B cells and potentially immunoglobulin-producing C-lambda+ plasma cells was observed in the lymphoid organs from the onset of disease. The increase in C-lambda+ B cells was matched by a parallel increase in IgM+ cells, which attained peak values from 7 days post-inoculation (dpi), while IgG+ cells increased from 11 dpi onwards. The enhanced biosynthetic capacity of these cells may have been linked to the initiation of a humoral response to CSF virus, and to the progressive decline in the albumin/globulin ratios of inoculated animals. Activation, proliferation and differentiation of B cells coincided with the presence of viral antigen, and with an intense phagocytic and biosynthetic activity of monocytes-macrophages and T lymphocytes. The previously reported increase of cytokine (TNFalpha, IL-1alpha and IL-6) production by monocytes-macrophages, and the release of IL-2, IL-4 and IFNgamma by T lymphocytes, may play a role in the initiation of the humoral immune response in CSF. These changes may have influenced the late appearance of virus-specific antibodies in the study, as well as the progressive increase of immunoglobulins.
authors.affiliation: Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Edificio de Sanidad Animal, Campus de Rabanales,14014 Córdoba, Spain.
authors.forename: P J
authors.initials: PJ
authors.lastname: Sánchez-Cordón
authors.forename: J L
authors.initials: JL
authors.lastname: Romero-Trevejo
authors.forename: M
authors.initials: M
authors.lastname: Pedrera
authors.forename: A I
authors.initials: AI
authors.lastname: Raya
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Villamandos
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D006691:Classical Swine Fever / Q000097:blood / Q000276:immunology* / Q000821:virology; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008221:Lymphoid Tissue / Q000276:immunology / Q000473:pathology; D008297:Male; D034421:Sus scrofa
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral