title: Development of a fluorescent intercalator displacement assay (G4-FID) for establishing quadruplex-DNA affinity and selectivity of putative ligands.
abstract: A fluorescent intercalator displacement assay (G4-FID) has been designed based on the displacement of thiazole orange (TO) positioned onto a quadruplex-forming oligonucleotide by putative ligands. This technique was validated by the use of a set of representative and fully characterized G-quadruplex binders (ranging from pyridodicarboxamide to macrocyclic ligands). To further extend its applicability, a comparative version has been developed which allows a rapid and viable determination of quadruplex- over duplex-selectivity.
authors.affiliation: Laboratoire de Chimie des Interactions Moléculaires, Collège de France, CNRS UPR285, 75005 Paris, France.
authors.forename: David
authors.initials: D
authors.lastname: Monchaud
authors.forename: Clémence
authors.initials: C
authors.lastname: Allain
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Teulade-Fichou
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D054856:G-Quadruplexes; D008024:Ligands; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D004247:DNA