Establishment and application of enzyme immunoassay for saliva cortisol in Taiwanese context.
Saliva steroid assay is an upcoming area of research, with much potential for growth and progress. Expensive, varying results with commercial kits and the disadvantages of radioimmunoassay have forced researchers to develop their own system of enzyme immunoassay (EIA). A modification from our established EIA system was used to develop a saliva cortisol (F) assay system. The system sensitivity (>90pg/mL) was checked by various experiments, including comparison of data with a commercial kit obtained from Salimetrics. The assay system was employed to investigate the saliva F level in a young Taiwanese population, and compared with the total and free serum levels of F.
Chen
Han-Lin
HL
Department of Medical Technology and Institute of Biotechnology, Yuanpei University of Science and Technology, Hsinchu, Taiwan, R.O.C.
Chen
Yung-Liang
YL
Wu
Leang-Shin
LS
Kaphle
Krishna
K
Lin
Jen-Hsou
JH
D000328:Adult; D044466:Asian People; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis*; D007124:Immunoenzyme Techniques / Q000379:methods*; D008297:Male; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013624:Taiwan
D006854:Hydrocortisone