Detecting fish parvalbumin with commercial mouse monoclonal anti-frog parvalbumin IgG.
Parvalbumin is a calcium-binding muscle protein that is highly conserved across fish species and amphibians. It is the major cross-reactive allergen associated with both fish and frog allergy. We used two-dimensional electrophoretic and immunoblotting techniques to investigate the utility of a commercial monoclonal anti-frog parvalbumin IgG for detecting parvalbumin present in some commonly consumed fish species. The 2D electrophoresis and immunoblots revealed species-specific differences in proteins that appear to represent various numbers of isoforms of parvalbumin in carp (5), catfish (3), cod (1) and tilapia (2). No parvalbumin was detected in yellowfin tuna. Based on minor differences in relative intensities of protein staining and immunodetection, parvalbumin isoforms may have slight differences in the epitope region recognized by the anti-frog parvalbumin antibody. These results suggest that the frog anti-parvalbumin antibody can be used as a valuable tool to detect parvalbumins from the fish tested in this study, except yellowfin tuna.
Chen
Lingyun
L
Food Allergy Research and Resource Program, University of Nebraska, Lincoln, Nebraska 68583-0919, USA, and Department of Pathophysiology, General Hospital, Medical University of Vienna, Wahringer Gurtel 18-20, Vienna A-1090, Austria.
Hefle
Sue L
SL
Taylor
Steve L
SL
Swoboda
Ines
I
Goodman
Richard E
RE
D000485:Allergens / Q000032:analysis; D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001001:Anura / Q000276:immunology; D002417:Cattle; D005399:Fishes / Q000276:immunology; D005512:Food Hypersensitivity; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010320:Parvalbumins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D051381:Rats; D016415:Sequence Alignment
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D010320:Parvalbumins