[HPLC-ESI/MS analysis of stachydrine and its metabolites in rat urine].
OBJECTIVE
To identify the main metabolites of stachydrine in rat.


METHODS
The ionization, cleavage and chromatographic characteristics of stachydrine were studied by using high-performance liquid chromatography-electrospray ionization ion trap tandem mass spectrometry (HPLC-ESI/MS) for the first time. These characteristics of stachydrine were used as the basis for the analyses of metabolites in rat urine. The 0 - 24 h urine samples of rats after ig 25 mg x kg(-1) stachydrine were collected and purified by using C10 solid-phase extraction cartridge, and then analyzed by HPLC-ESI/MS to identify stachydrine and its metabolites.


RESULTS
The parent drug (stachydrine), 6 phase I metabolites (N-demethyl, dehydrogenation, ring-oxidation) and 2 phase II metabolites (glycine conjugates of 2 ring-oxidation products) were identified existing in rat urine.


CONCLUSIONS
The presented method was proved to be sensitive, rapid, high selective and specific for the identification of stachydrine and its metabolites in rat urine.
Chen
Huai-Xia
HX
Hubei Province Key Lab of Bio-Technology of Traditional Chinese Medicine, Hubei University, Wuhan 430062, China.
Shen
Shao-Lin
SL
Han
Feng-Mei
FM
Chen
Yong
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011392:Proline / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D031366:Stachys / Q000737:chemistry
D011392:Proline; C003342:stachydrine