title: Recommended method for recovery of Toxocara and other geohelminth eggs from soil.
abstract: The flotation method elaborated for recovery of Toxocara and other geohelminth eggs from soil is described. Soil samples of about 500 ml volume are picked from 3-cm superficial layer of the ground. In the laboratory, 40 g of dry and sifted material is analysed according to following procedure: 1 h standing, 20 minutes shaking and 3 minutes centrifugation (1500 rpm) in 5% sodium hydroxide (NaOH), then centrifugation, like above, with H2O for washing the sample and next with the saturated sodium nitrate (NaNO3) for flotation the eggs. Specimen is prepared by placing a cover slip on the positive meniscus of the flotation liquid.
authors.affiliation: Department of Biology and Environmental Protection, Eugeniusz Piasecki University School of Physical Education, Królowej Jadwigi 27/39, 61-871 Poznań. mizgajska@awf.poznan.pl
authors.forename: Hanna
authors.initials: H
authors.lastname: Mizgajska-Wiktor
mesh_terms: D000818:Animals; D004463:Ecology / Q000379:methods; D010063:Ovum; D012987:Soil / Q000032:analysis* / Q000469:parasitology*; D013048:Specimen Handling; D014119:Toxocara / Q000302:isolation & purification*
chemical_list: D012987:Soil