title: [Integration between M3 muscarinic acetylcholine receptor and connexin 43 as antiarrhythmic targets in rat ventricular myocardium].
abstract: OBJECTIVE
  To optimize the method of investigating structural integration between proteins and study the integration between arrhythmia related proteins in molecular level.
  
  
  METHODS
  Immunostaining the normal ventricular myocytes was used to observe the distribution of connexin 43 and muscarinic acetylcholine receptor (mAChR). The five mAChR subtypes were precipitated using immunoprecipitation. Then, SDS-PAGE and Western blotting with the anti-connexin 43 antibody were performed to observe whether they were structurally integrated. Further, different concentrations of detergent were used to observe whether this relationship could be broken.
  
  
  RESULTS
  The five subtypes of mAChR existed in the cardiac myocyte of the rat, and all the five mAChR subtypes combined with connexin 43. In the normal rat ventricular myocyte membrane, connexin 43 and M3 receptor are co-located. When adding certain concentration of detergent to the membrane protein, the integration between M3 receptor and connexin 43 was broken, and the phosphorylated form of connexin 43 integrated with M3 receptor.
  
  
  CONCLUSIONS
  The results indicated that the structural integration between mAChR and phosphorylation of connexin 43 existed in rat ventricular myocardium, and this integration could be broken by certain concentration of detergent.
authors.affiliation: Department of Pharmacology, Bio-pharmaceutical Key Laboratory of Heilongjiang Province-Incubator of State Key Laboratory, Harbin Medical University, Harbin 150086, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Yue
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Yu
authors.forename: Zhen-Wei
authors.initials: ZW
authors.lastname: Pan
authors.forename: Dao-Hong
authors.initials: DH
authors.lastname: Lin
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Lü
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Yang
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D018031:Connexin 43 / Q000378:metabolism*; D006352:Heart Ventricles; D047468:Immunoprecipitation; D008297:Male; D018613:Microscopy, Confocal; D032383:Myocytes, Cardiac / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D043587:Receptor, Muscarinic M3 / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: D018031:Connexin 43; D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic; D012967:Sodium Dodecyl Sulfate