title: Isoelectric focusing sample injection for capillary zone electrophoresis in a fused silica capillary.
abstract: An improvement has been made to couple isoelectric focusing (IEF) sample injection and capillary zone electrophoresis in an untreated fused silica capillary. Electroosmotic flow is efficiently prevented by simply using a rubber block at the outlet end of the capillary during IEF sample injection. The experimental conditions that affect the concentration effect are discussed. A concentration enhancement factor of over 100-fold can be easily obtained for two model proteins: lysozyme and ribonuclease A.
authors.affiliation: Department of Materials Science and Engineering, Faculty of Engineering, University of Fukui, Bunkyo 3-9-1. Fukui 910-8507, Japan.
authors.forename: Luo-Hong
authors.initials: LH
authors.lastname: Zhang
authors.forename: Cheng-Jie
authors.initials: CJ
authors.lastname: Zhang
authors.forename: Xing-Zheng
authors.initials: XZ
authors.lastname: Wu
mesh_terms: D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D009113:Muramidase / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D012822:Silicon Dioxide; D012259:Ribonuclease, Pancreatic; D009113:Muramidase