Enhanced survival of GroESL-overproducing Lactobacillus paracasei NFBC 338 under stressful conditions induced by drying.
GroESL-overproducing Lactobacillus paracasei NFBC 338 was dried, and its viability was compared with that of controls. Spray- and freeze-dried cultures overproducing GroESL exhibited approximately 10-fold and 2-fold better survival, respectively, demonstrating the importance of GroESL in stress tolerance, which can be exploited to enhance the technological performance of sensitive probiotic cultures.
Corcoran
B M
BM
Teagasc, Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland.
Ross
R P
RP
Fitzgerald
G F
GF
Dockery
P
P
Stanton
C
C
D001426:Bacterial Proteins / Q000378:metabolism*; D018833:Chaperonins / Q000378:metabolism*; D015169:Colony Count, Microbial; D003470:Culture Media; D005612:Freeze Drying / Q000379:methods*; D018869:Heat-Shock Response; D007778:Lactobacillus / Q000254:growth & development* / Q000378:metabolism; D019936:Probiotics; D015854:Up-Regulation
D001426:Bacterial Proteins; D003470:Culture Media; C097970:GroESL protein, Bacteria; D018833:Chaperonins