title: Identification of genes of the plant-specific transcription-factor families cooperatively regulated by ethylene and jasmonate in Arabidopsis thaliana.
abstract: The analysis of expression patterns of transcription-factor genes will be the basis for a better understanding of their biological functions in plants. In this study, we designed and developed an oligo-DNA macroarray consisting of gene-specific probes of 60-65 nucleotides for 288 transcription-factor genes, which cover COL, DOF, ERF, and NAC family genes. To investigate transcription-factor genes that are cooperatively regulated by jasmonate and ethylene in arabidopsis (Arabidopsis thaliana (L.) Heynh.) plants, we analyzed the expression profile of transcription-factor genes using the oligo-DNA macroarray technique in arabidopsis plants treated with methyl jasmonate and 1-aminocyclopropane-1-carboxylic acid. Then, transcript levels of candidate genes-which were selected based on the result of macroarray analysis-were evaluated by the quantitative real-time RT-PCR method. Finally, we identified an ERF family gene that is cooperatively regulated by both hormones, and designated as cooperatively regulated by ethylene and jasmonate 1 (CEJ1).
authors.affiliation: Molecular and Cellular Breeding Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Nakano
authors.forename: Kaoru
authors.initials: K
authors.lastname: Suzuki
authors.forename: Namie
authors.initials: N
authors.lastname: Ohtsuki
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Tsujimoto
authors.forename: Tatsuhito
authors.initials: T
authors.lastname: Fujimura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shinshi
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics*; D003517:Cyclopentanes / Q000494:pharmacology*; D005030:Ethylenes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D014157:Transcription Factors; C011006:jasmonic acid; C036216:ethylene