The effect of FF-MAS on porcine cumulus-oocyte complex maturation, fertilization and pronucleus formation in vitro.
Follicular fluid meiosis-activating sterol (FF-MAS) has been isolated from the follicular fluid (FF) of several species including man. FF-MAS increases the quality of in vitro oocyte maturation, and thus the developmental potential of oocytes exposed to FF-MAS during in vitro maturation is improved. The aim of the present study was to investigate the effects of FF-MAS on porcine oocyte maturation and pronucleus formation in vitro. Porcine cumulus-oocyte complexes (COCs) were isolated from abattoir ovaries and in vitro matured for 48 h in NCSU 37 medium supplemented with 1 mg/l cysteine, 10 ng/ml epidermal growth factor and 50 microM 2-mercaptoethanol with or without 10% porcine follicular fluid (pFF). For the first 22 h, 1 mM db-cAMP and 10 I.E PMSG/hCG was added. The medium was supplemented with 1 microM, 3 microM, 10 microM, 30 microM or 100 microM FF-MAS dissolved in ethanol. After maturation the COCs were denuded mechanically using a fine glass pipette under constant pH and in vitro fertilized with fresh semen (5 x 10(5) spermatozoa/ml). The presumptive zygotes were evaluated 18 h after fertilization. The addition of pFF increased the monospermic as well as the polyspermic penetration of oocytes. In the absence of pFF, the addition of FF-MAS decreased the polyspermic penetration rate, whereas FF-MAS in combination with pFF decreased monospermic and increased polyspermic penetration. The degeneration rate of ova decreased in the presence of FF-MAS irrespective of the presence or absence of pFF. In the absence of pFF, FF-MAS at 3-10 microM increased the number of zygotes with advanced maternal pronuclear stages. In supraphysiological doses, i.e. 30-100 microM, FF-MAS dose-dependently and reversibly inhibited nuclear maturation in the absence of pFF.
Faerge
Inger
I
Fertility Team, Novo Nordisk A/S, Copenhagen, Denmark. if@kvl.dk
Strejcek
Frantisek
F
Laurincik
Jozef
J
Rath
Detlef
D
Niemann
Heiner
H
Schellander
Karl
K
Rosenkranz
Christine
C
Hyttel
Poul Maddox
PM
Grøndahl
Christian
C
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects*; D002782:Cholestenes / Q000494:pharmacology*; D005307:Fertilization in Vitro / Q000187:drug effects*; D009865:Oocytes / Q000187:drug effects*; D013552:Swine; D015053:Zygote / Q000187:drug effects
D002782:Cholestenes; C100324:4,4-dimethylcholesta-8,14,24-trienol