title: Micropatterning with aerosols: application for biomaterials.
abstract: Adhesion and proliferation behaviors of bovine aortic endothelial cells (BAECs) were investigated on surfaces micropatterned with peptides using a novel approach. This micropatterning technique allows modification of macroscopic three-dimensional (3D) biomaterials surfaces and exploits the semi-random properties of aerosols and the principles of liquid atomization. The possibility to control cell behaviors on polytetrafluoroethylene (PTFE) surfaces tailored with this micropatterning approach was evaluated. CGRGDS and CWQPPRARI peptides were selected for their adhesive, migration and spreading properties. Culture of BAECs on patterned PTFE showed the possibility of modulating cell behaviors. The study showed that CGRGDS spots with a diameter of 10+/-2 microm over a background of CWQPPRARI peptides was the most effective combination to enhance endothelialization of PTFE. This micropatterning technique is innovative, easily adaptable, simple, and rapid for covering large 3D areas.
authors.affiliation: Unité de biotechnologie et de bioingénierie, Centre de recherche du CHUQ, Hôpital Saint-François d'Assise, and Département de génie des mines, de la métallurgie et des matériaux, Faculté des sciences et de génie, Université Laval, Qué., Canada.
authors.forename: Louis
authors.initials: L
authors.lastname: Gagné
authors.forename: Gerardo
authors.initials: G
authors.lastname: Rivera
authors.forename: Gaetan
authors.initials: G
authors.lastname: Laroche
mesh_terms: D000336:Aerosols / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry*; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000336:Aerosols; D001672:Biocompatible Materials; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D011138:Polytetrafluoroethylene