title: Immunological images of polycyclic aromatic hydrocarbons.
abstract: OBJECTIVE
  To develop experimental model for definition of immunological images of chemical carcinogens.
  
  
  METHODS
  The conjugates of benzo[a]pyrene, benz[a]anthracene, anthracene, chrysene and pyrene with bovine serum albumin and yeast hexokinase were synthesized. Rabbits were immunized by bovine serum albumin-hapten conjugates. Antibodies to each hapten were isolated from the serum by affinity chromatography with the hapten-yeast hexokinase-Sepharose sorbents. The binding of each hapten with each antibody was determined by competitive immunoassay.
  
  
  RESULTS
  The immunological images of all the investigated chemical compounds were described.
  
  
  CONCLUSIONS
  The model is proposed to determine the internal immunological images of anti-idiotypic monoclonal antibodies to chemical carcinogens.
authors.affiliation: Institute of Human Ecology, Siberian Branch of Russian Academy of Sciences, Kemerovo, Russia. ihe@kemtel.ru
authors.forename: A N
authors.initials: AN
authors.lastname: Glushkov
authors.forename: M V
authors.initials: MV
authors.lastname: Kostyanko
authors.forename: T P
authors.initials: TP
authors.lastname: Anosova
authors.forename: E G
authors.initials: EG
authors.lastname: Polenok
authors.forename: S A
authors.initials: SA
authors.lastname: Mun
authors.forename: M P
authors.initials: MP
authors.lastname: Anosov
authors.forename: S V
authors.initials: SV
authors.lastname: Cherno
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000302:isolation & purification; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002273:Carcinogens / Q000737:chemistry; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D006593:Hexokinase / Q000737:chemistry / Q000276:immunology; D007114:Immunization; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology
chemical_list: D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002273:Carcinogens; D006241:Haptens; D011084:Polycyclic Aromatic Hydrocarbons; D012710:Serum Albumin, Bovine; D006593:Hexokinase