title: Cell-free expression systems for eukaryotic protein production.
abstract: Following the success of genome sequencing projects, attention has now turned to studies of the structure and function of proteins. Although cell-based expression systems for protein production have been widely used, they have certain limitations in terms of the quality and quantity of the proteins produced and for high-throughput production. Many of these limitations can be circumvented by the use of cell-free translation systems. Among such systems, the wheat germ based system is of special interest for its eukaryotic nature; it has the significant advantage of producing eukaryotic multidomain proteins in a folded state. Several advances in the use of cell-free expression systems have been made in the past few years and successful applications of these systems to produce proteins for functional and structural biology studies have been reported.
authors.affiliation: Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Japan. yendo@eng.ehime-u.ac.jp
authors.forename: Yaeta
authors.initials: Y
authors.lastname: Endo
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sawasaki
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods*; D002474:Cell-Free System / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D040901:Proteomics / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D011994:Recombinant Proteins