title: Interaction of phosphorylase kinase from rabbit skeletal muscle with flavin adenine dinucleotide.
abstract: The interaction of flavin adenine dinucleotide (FAD) with rabbit skeletal muscle phosphorylase kinase has been studied. Direct evidence of binding of phosphorylase kinase with FAD has been obtained using analytical ultracentrifugation. It has been shown that FAD prevents the formation of the enzyme-glycogen complex, but exerts practically no effect on the phosphorylase kinase activity. The dependence of the relative rate of phosphorylase kinase-glycogen complex formation on the concentration of FAD has cooperative character (the Hill coefficient is 1.3). Under crowding conditions in the presence of 1 M trimethylamine-N-oxide (TMAO), FAD has an inhibitory effect on self-association of phosphorylase kinase. The data suggest that the complex of glycogen metabolism enzymes in protein-glycogen particles may function as a flavin depot in skeletal muscle.
authors.affiliation: Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia. makeeva@inbi.ras.ru
authors.forename: V F
authors.initials: VF
authors.lastname: Makeeva
authors.forename: N A
authors.initials: NA
authors.lastname: Chebotareva
authors.forename: I E
authors.initials: IE
authors.lastname: Andreeva
authors.forename: N B
authors.initials: NB
authors.lastname: Livanova
authors.forename: B I
authors.initials: BI
authors.lastname: Kurganov
mesh_terms: D000818:Animals; D019281:Dimerization; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D008744:Methylamines / Q000494:pharmacology; D046912:Multiprotein Complexes / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology*; D009994:Osmolar Concentration; D010764:Phosphorylase Kinase / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D011817:Rabbits; D013552:Swine; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D008744:Methylamines; D046912:Multiprotein Complexes; D005182:Flavin-Adenine Dinucleotide; D006003:Glycogen; D010764:Phosphorylase Kinase; C005855:trimethyloxamine