[Detection of RNA in vaccine " EnceVir" against tick-borne encephalitis virus].
Fractionation of RNA isolated from the inactivated purified concentrated vaccine "EnceVir" ("Virion", Tomsk, Russia) allowed the authors to reveal predominantly double-stranded RNA and trace quantities of single-stranded RNA sizing mainly less than 200 n. The fragments of tick-borne encephalitis virus (TBEV) were detected among the total RNA by the reverse transcription test, followed by nested polymerase chain reaction with the primers specific to the E gene of TBEV. The findings may suggest that immunity may be induced through activation of Th1 and Th2 after immunization with such an inactivated vaccine for effective elimination of intracellular infection and intracellular virions, respectively.
Morozova
O V
OV
Tkachev
S E
SE
Chudinov
A P
AP
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics / Q000276:immunology*; D004675:Encephalitis, Tick-Borne / Q000517:prevention & control; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014765:Viral Vaccines / Q000592:standards*
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014765:Viral Vaccines