title: Investigating cis-acting regulatory variation using assays of relative allelic expression.
abstract: Assays of relative allelic expression offer a powerful means of exploring the influence of cis-acting regulatory variation. In this article, we provide a general assay protocol and discuss practical and theoretical considerations for the design and interpretation of relative allelic expression analysis.
authors.affiliation: Department of Psychological Medicine, School of Medicine, Cardiff University, Cardiff, UK. braynj@cardiff.ac.uk
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Bray
authors.forename: Michael C
authors.initials: MC
authors.lastname: O'Donovan
mesh_terms: D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006801:Humans; D008957:Models, Genetic; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012313:RNA; D004247:DNA