NMR spectroscopic study of a DNA duplex with mercury-mediated T-T base pairs.
Recently, we reported that T-T mismatches can specifically recognize Hg(II) (T-Hg(II)-T pair formation). In order to understand the properties of the T-Hg(II)-T pair, we recorded NMR spectra for a DNA duplex, d(CGCGTTGTCC).d(GGACTTCGCG), with two successive T-T mismatches (Hg (II)-binding sites). We assigned 1H resonances for mercury-free and di-mercurated duplexes, and performed titration experiments with Hg(II) by using 1D 1H NMR spectra. Because of the above mentioned assignments, we could confirm the existence of mono-mercurated species, because individual components gave independent NMR signals in the titration spectra.
Tanaka
Yoshiyuki
Y
Laboratory of Molecular Transformation, Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan. tanaka@mail.pahrm.tokoku.ac.jp
Yamaguchi
Hiroshi
H
Oda
Shuji
S
Kondo
Yoshinori
Y
Nomura
Makoto
M
Chojiro
Kojima
K
Akira
Ono
O
D001482:Base Composition; D020029:Base Pairing / Q000187:drug effects; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008628:Mercury / Q000737:chemistry* / Q000494:pharmacology; D008958:Models, Molecular; D013941:Thymine / Q000737:chemistry*
D004247:DNA; D008628:Mercury; D013941:Thymine