title: Antioxidants enhanced production of destruxin E from cultivation of Metarhizium anisopliae.
abstract: The effect of antioxidants on the production of an important cyclohexadepsipeptide congener destruxin E (dtx E) was investigated using the entomopathogenic fungus Metarhizium anisopliae F061. In shaker flask cultivations, 0.015% of menadione-enhanced dtx E production of 220.4 mg/l compared to the control cultivation 90.2 mg/l, which was illustrated by stimulation of dtx E biosynthesis through two electron reduction DT-diaphorase processes in cultivation of M. anisopliae. In 5-l stirred-tank bioreactor cultivation with menadione addition and of control pH 4.0, a yield of 454.6 mg/l of dtx E was obtained after 7 days, and was nearly 30 and 15-fold higher than that from no pH control, and controlled pH 2.0 cultivations, respectively. Further cultivation in a 20-l airlift bioreactor, at pH 4.0, dtx E obtained on the 9th day was 406.0 mg/l, which was much higher than the standard cultivation of no pH control yield 203.3 mg/l on the 11th day. Thus, the present study provides useful information for enhancing dtx E production in cultivation.
authors.affiliation: Institute of Biotechnology, Chaoyang University of Technology, Wufeng 41349, Taiwan, Republic of China.
authors.forename: Yerra Koteswara
authors.initials: YK
authors.lastname: Rao
authors.forename: Chia-Heng
authors.initials: CH
authors.lastname: Tsou
authors.forename: Yew-Min
authors.initials: YM
authors.lastname: Tzeng
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D019149:Bioreactors; D047630:Depsipeptides / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D052981:Metarhizium / Q000254:growth & development / Q000378:metabolism*; D009183:Mycotoxins / Q000096:biosynthesis; D024483:Vitamin K 3 / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D047630:Depsipeptides; D005656:Fungal Proteins; D009183:Mycotoxins; D024483:Vitamin K 3; C040425:destruxin E