title: Assessment of viability and osteogenic ability of human mesenchymal stem cells after being stored in suspension for clinical transplantation.
abstract: Human mesenchymal stem cells (MSCs) were suspended in phosphate-buffered saline (PBS) and stored up to 24 h at 4 degrees C, 24 degrees C, and 37 degrees C. More than 80% viability was maintained at any temperature for at least 1 h, then gradually decreased over time. After 24 h, the viabilities at 4 degrees C, 24 degrees C, and 37 degrees C were about 81%, 70%, and 62%, respectively. The MSCs suspended/stored in PBS at 4 degrees C for 24 h also exhibited in vitro osteogenic differentiation capability as evidenced by mineralized matrix formation as well as high alkaline phosphatase activity when cultured in an osteogenic medium. Furthermore, in vivo implantation experiments using the MSCs also demonstrated new bone formation. Because MSCs are known to possess multipotential stem cell characteristics, these data indicate that human MSCs stored in PBS at 4 degrees C could be delivered to distant medical facilities for the purpose of hard tissue and other types of tissue regeneration therapy.
authors.affiliation: Tissue Engineering Research Group, Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST) Hyogo, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Muraki
authors.forename: Motohiro
authors.initials: M
authors.lastname: Hirose
authors.forename: Noriko
authors.initials: N
authors.lastname: Kotobuki
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kato
authors.forename: Hiroko
authors.initials: H
authors.lastname: Machida
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Takakura
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohgushi
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods / Q000592:standards; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D013696:Temperature