Biolistic inoculation of soybean plants with soybean dwarf virus.
Soybean dwarf virus (SbDV), a member of the genus Luteovirus, has been transmitted only by aphid vectors. The possibility of using a biolistic procedure of transmitting SbDV to soybean plants without relying on aphid vectors was investigated. Biolistic inoculation using the Helios Gene Gun System with RNA transcribed in vitro from a full-length cDNA clone of the DS strain of SbDV (pSV-DS) resulted in 1/12 to 3/13 soybean plants infected systemically. The infected soybean plants showed the characteristic symptoms of SbDV-DS within 6 weeks after inoculation and the accumulation of SbDV-specific RNA species such as genomic and subgenomic RNAs in the upper non-inoculated leaves. The progeny virus derived from RNA transcribed in vitro from pSV-DS could be transmitted by aphid vectors, as is the case with native SbDV-DS. This is the first report of direct inoculation of soybean plants with SbDV without using aphid vectors.
Yamagishi
Noriko
N
Department of Crop Breeding, National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020-0198, Japan.
Terauchi
Hidetaka
H
Kanematsu
Seiji
S
Hidaka
Soh
S
D019470:Biolistics / Q000379:methods*; D018076:DNA, Complementary; D017793:Luteovirus / Q000235:genetics* / Q000502:physiology*; D018515:Plant Leaves / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D013025:Glycine max / Q000821:virology*
D018076:DNA, Complementary; D012367:RNA, Viral