title: The Te-Assay: a black and white method for environmental sample pre-screening exploiting tellurite reduction.
abstract: We present the tellurite bioassay (Te-Assay) as an alternative approach for quantification of cell viability. The Te-Assay was developed to pre-screen environmental samples for potential bacterial toxicants in which the reduction of tellurite to tellurium is used as a metabolic marker; black phenotype development only occurs in metabolically competent bacteria capable of reducing tellurite (TeO(3)(2-)) to elemental tellurium. The black and white phenotypes equate to nonsignificant or significant impediment of normal metabolic processes, thus permitting the rapid visual assessment of the relative toxicity of environmental samples. Bacterial inocula were exposed in 96-well plates to arrays of diluted analytes or environmental samples before addition of a tellurite to assess cell health/viability. Toxicity was quantified as the analyte concentration at which a 50% reduction in blackness occurred (IC(50)) compared to control wells containing no added analyte. No proprietary strains or reagents are required for Te-Assay, in which characterised strains or recent environmental isolates performed equally well. Strain selection was independent of tellurite-resistance provided that tellurite was reduced intracellularly by active non-growing cells.
authors.affiliation: Landcare Research, P.O. Box 69, Lincoln 8152, New Zealand; Scion, Te Papa Tipu Innovation Park, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand. gareth.lloyd-jones@scionresearch.com <gareth.lloyd-jones@scionresearch.com>
authors.forename: Gareth
authors.initials: G
authors.lastname: Lloyd-Jones
authors.forename: Wendy M
authors.initials: WM
authors.lastname: Williamson
authors.forename: Tineke
authors.initials: T
authors.lastname: Slootweg
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods*; D003116:Color; D004787:Environmental Pollution / Q000032:analysis*; D050296:Microbial Viability; D010084:Oxidation-Reduction; D010641:Phenotype; D013691:Tellurium / Q000378:metabolism*
chemical_list: C026660:tellurous acid; D013691:Tellurium