title: Results of gastroscope bacterial decontamination by enzymatic detergent compared to chlorhexidine.
abstract: OBJECTIVE
  To compare the efficacy of enzymatic detergent with chlorhexidine for gastroscope bacterial decontamination.
  
  
  METHODS
  A prospective randomized controlled study was undertaken to evaluate the ability of these 2 agents to achieve high level disinfection in a gastroscope. A total of 260 samples were collected from 5 different gastroscopes. Manual cleaning was done for 10 min with these 2 agents separately (n = 130 each). Then all specimens underwent 2% glutaraldehyde soaking for 20 min. After 70% alcohol was rinsed, sterile normal saline was flushed into each gastroscope channel and 40 mL of sample was collected. The sample was sent for aerobic bacterial culture after membrane was filtered. A colony count greater than 200 cfu/mL was considered significant.
  
  
  RESULTS
  The positive culture rate was 4.6% in the enzymatic detergent arm and 3.1% in the chlorhexidine arm. Pseudomonas species were the main organism detected from both groups (60%). Multiple organisms were found from 4 specimens (enzymatic detergent arm = 1, chlorhexidine arm = 3).
  
  
  CONCLUSIONS
  The contamination rate of both types of cleaning solution is equivalent.
authors.affiliation: Gastroenterology Unit, Department of Internal Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand. rungsun@pol.net
authors.forename: Rungsun
authors.initials: R
authors.lastname: Rerknimitr
authors.forename: Sorapat
authors.initials: S
authors.lastname: Eakthunyasakul
authors.forename: Pongpan
authors.initials: P
authors.lastname: Nunthapisud
authors.forename: Pradermchai
authors.initials: P
authors.lastname: Kongkam
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects; D002710:Chlorhexidine / Q000494:pharmacology*; D003666:Decontamination / Q000379:methods*; D003902:Detergents / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D004754:Enterobacter / Q000187:drug effects; D004798:Enzymes / Q000494:pharmacology*; D004866:Equipment Contamination; D020699:Gastroscopes / Q000382:microbiology*; D006801:Humans; D007709:Klebsiella / Q000187:drug effects; D011446:Prospective Studies; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D003902:Detergents; D004202:Disinfectants; D004798:Enzymes; D002710:Chlorhexidine