title: NMR spectroscopic study of a DNA duplex with mercury-mediated T-T base pairs.
abstract: Recently, we reported that T-T mismatches can specifically recognize Hg(II) (T-Hg(II)-T pair formation). In order to understand the properties of the T-Hg(II)-T pair, we recorded NMR spectra for a DNA duplex, d(CGCGTTGTCC).d(GGACTTCGCG), with two successive T-T mismatches (Hg (II)-binding sites). We assigned 1H resonances for mercury-free and di-mercurated duplexes, and performed titration experiments with Hg(II) by using 1D 1H NMR spectra. Because of the above mentioned assignments, we could confirm the existence of mono-mercurated species, because individual components gave independent NMR signals in the titration spectra.
authors.affiliation: Laboratory of Molecular Transformation, Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan. tanaka@mail.pahrm.tokoku.ac.jp
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Shuji
authors.initials: S
authors.lastname: Oda
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kondo
authors.forename: Makoto
authors.initials: M
authors.lastname: Nomura
authors.forename: Kojima
authors.initials: K
authors.lastname: Chojiro
authors.forename: Ono
authors.initials: O
authors.lastname: Akira
mesh_terms: D001482:Base Composition; D020029:Base Pairing / Q000187:drug effects; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008628:Mercury / Q000737:chemistry* / Q000494:pharmacology; D008958:Models, Molecular; D013941:Thymine / Q000737:chemistry*
chemical_list: D004247:DNA; D008628:Mercury; D013941:Thymine