Comparison of the characterization on binding of alpinetin and cardamonin to lysozyme by spectroscopic methods.
Studies on the binding affinity of protein to the active components of herbs are novel in biochemistry and are valuable for the information about speciation of drugs and exchange in biological systems. Alpinetin and cardamonin, two of the main constituents from the seeds of Alpinia katsumadai Hayata, have been used in traditional herbs as antibacterial, anti-inflammatory, and other important therapeutic activities of significant potency and low systemic toxicity. The interactions between two flavonoids analogs and lysozyme have been studied for the first time by spectroscopic method including Fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) and UV-absorption spectroscopy in combination with Fluorescence quenching study. Both molecules showed high affinities to lysozyme under the experimental condition with drug concentrations from 3.33 x 10(-6) to 2.67 x 10(-5)molL(-1) for alpinetin and 1.67 x 10(-6) to 13.33 x 10(-6)molL(-1) for cardamonin. The alterations of protein secondary structure in the presence of drugs in aqueous solution were quantitatively estimated by the evidences from CD and FT-IR spectroscopy. The thermodynamic parameters obtained and the results of spectroscopic measurements suggest that hydrophobic and electrostatic interactions are the predominant intermolecular forces stabilizing two coordination compounds. The quenching mechanism and the number of binding site (n approximately 1) were obtained by fluorescence titration data. The efficiency of energy transfer provided the binding distances of 4.04 and 5.90 nm for alpinetin-LYSO and cardamonin-LYSO systems, respectively.
He
Wenying
W
Department of Chemistry, Lanzhou University, Lanzhou, China.
Li
Ying
Y
Tang
Jianghong
J
Luan
Feng
F
Jin
Jing
J
Hu
Zhide
Z
D032429:Alpinia / Q000737:chemistry; D001665:Binding Sites; D047188:Chalcones / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D044950:Flavanones / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
D047188:Chalcones; D044950:Flavanones; C436748:alpinetin; D009113:Muramidase; C436747:cardamonin