[Determination of organophosphorus pesticide residues in tobacco by high performance liquid chromatography-tandem mass spectrometry].
A novel and rapid method based on high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for determination of organophosphorus pesticide residues (OPs) in tobacco. The OPs were extracted from tobacco samples by acetonitrile under ultrasonication. The extractive was determined by high performance liquid chromatography using methanol-water (containing 0.1% ammonium acetate) (95:5, v/v) as mobile phase. The OPs were detected using a tandem mass spectrometer with electrospray ionization in multiple reaction monitoring (MRM) mode. The OPs, such as dimethoate, trichlorfon, chlorpyrifos, methamidophos and acephate could be detected quantitatively within 2.5 min. The linear calibration curves were obtained in the entire range (1-200 microg/L) for all the above pesticides (r > 0.998). The average recoveries in tobacco obtained were ranged from 77% to 104%. The limits of detection were between 1.0 microg/kg and 5.0 microg/kg.
Liu
Yingwen
Y
College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
Ding
Shichao
S
Du
Wen
W
Yin
Donghong
D
Liu
Jianfu
J