Trapping of the OH radical by alpha-tocopherol: a theoretical study.
The antioxidant activity of alpha-tocopherol against the damaging hydroxyl radical was analyzed theoretically by hybrid density functional theory, following the direct dynamics method, where the thermal rate constants were calculated using variational transition-state theory with multidimensional tunneling. We found that the OH radical is only slightly or not at all selective, attacking by different mechanisms at several positions of the alpha-tocopherol molecule, giving competitive reactions. The most favorable pathways are the hydrogen abstraction reaction from the phenolic hydrogen and the electrophilic addition onto the aromatic ring. We propose a final rate constant, the sum of the competitive hydrogen abstraction and addition reactions, > or =2.7 x 10(8) M(-1) s(-1) at 298 K, where the hydrogen abstraction reaction represents only 20% of the total OH radical reaction. This result indicates that, molecule by molecule, in an apolar environment, alpha-tocopherol is less effective than coenzyme Q (which presents a rate constant of 6.2 x 10(10) M(-1) s(-1) at 298 K) as a scavenger of OH radicals. It was also found that both mechanisms are not direct but pass through intermediates in the entry channel, with little or no influence on the dynamics of the reactions. The hydrogen abstraction reaction also presents another intermediate in the exit channel, which may have a significant role in preventing the pro-oxidant effects of alpha-tocopherol, although less important than with free radicals other than OH.
Navarrete
M
M
Departamento de Química Física, Universidad de Extremadura, 06071 Badajoz, Spain.
Rangel
C
C
Corchado
J C
JC
Espinosa-García
J
J
D000975:Antioxidants / Q000737:chemistry; D003198:Computer Simulation; D004583:Electrons; D005609:Free Radicals / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D006878:Hydroxides / Q000737:chemistry*; D007700:Kinetics; D015394:Molecular Structure; D013696:Temperature; D024502:alpha-Tocopherol / Q000737:chemistry*
D000975:Antioxidants; D005609:Free Radicals; D006878:Hydroxides; D006859:Hydrogen; C031356:hydroxide ion; D024502:alpha-Tocopherol