Presence of neutrophil-bearing antigen in lymphoid organs of immune mice.
Neutrophils play a crucial early role during the innate response, but little is known about their possible contribution when an adaptive immune response is installed. A robust neutrophilia and a T helper 1 (Th1) immune response are present after immunization with Complete Freund Adjuvant (CFA). We show that when FITC-labeled OVA was injected into the footpad of OVA/CFA immunized mice, the main OVA-FITC+ cells recruited in draining popliteal lymph nodes (LNs) were neutrophils, with most of them arriving at the LN by means of lymphatic vessels. The development of this OVA-FITC+ neutrophil influx requires an immune response against OVA. The OVA-FITC+ neutrophils present in LNs displayed mainly intracellular TNF-alpha, and their depletion resulted in an increase in the specific IL-5 levels. These data provide new evidence about the role played by neutrophils in vivo in adaptive immunity.
Maletto
Belkys A
BA
Centro de Investigaciones en Bioquímica Clínica e Inmunología-Consejo Nacional de Investigaciones Científicas y Técnicas (CIBICI CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Ropolo
Andrea S
AS
Alignani
Diego O
DO
Liscovsky
Miriam V
MV
Ranocchia
Romina P
RP
Moron
Victor Gabriel
VG
Pistoresi-Palencia
María C
MC
D000818:Animals; D000917:Antibody Formation; D000936:Antigen-Antibody Complex / Q000276:immunology; D017209:Apoptosis; D016207:Cytokines / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D007114:Immunization; D008198:Lymph Nodes / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology
D000936:Antigen-Antibody Complex; D016207:Cytokines; D010047:Ovalbumin