title: A functional mobA gene for molybdopterin cytosine dinucleotide cofactor biosynthesis is required for activity and holoenzyme assembly of the heterotrimeric nicotine dehydrogenases of Arthrobacter nicotinovorans.
abstract: Two Arthrobacter nicotinovorans molybdenum enzymes hydroxylate the pyridine ring of nicotine. Molybdopterin cytosine dinucleotide (MCD) was determined to be a cofactor of these enzymes. A mobA gene responsible for the formation of MCD could be identified and its function shown to be required for assembly of the heterotrimeric molybdenum enzymes.
authors.affiliation: Institute for Biochemistry and Molecular Biology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany.
authors.forename: Paula
authors.initials: P
authors.lastname: Sachelaru
authors.forename: Emile
authors.initials: E
authors.lastname: Schiltz
authors.forename: Roderich
authors.initials: R
authors.lastname: Brandsch
mesh_terms: D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D003597:Cytosine Nucleotides / Q000096:biosynthesis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D019281:Dimerization; D015964:Gene Expression Regulation, Bacterial; D020035:Holoenzymes / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D011622:Pterins; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D003597:Cytosine Nucleotides; D004268:DNA-Binding Proteins; D020035:Holoenzymes; D011622:Pterins; D015534:Trans-Activators; C066895:molybdopterin cytosine dinucleotide; D000587:Oxidoreductases Acting on CH-NH Group Donors; C066274:nicotine dehydrogenase