Determining sulfamonomethoxine and its acetyl/hydroxyl metabolites in chicken plasma under organic solvent-free conditions.
A quantitative technique is described for a sample preparation followed by high performance liquid chromatography method for the simultaneous determination of sulfamonomethoxine and its metabolites, N4-acetyl SMM and 2,6-dihydroxy SMM, in chicken plasma. The average recoveries, analytical total time, and limits of quantitation were >/=80% (relative standard deviations (SD) </=6%), <30 min sample-1 (12 samples in 2 h), and </=0.09 microg ml(-1), respectively. The procedure, performed under 100% aqueous conditions, uses no organic solvents and toxic reagents at all and is, therefore, harmless to the environment and humans.
Furusawa
Naoto
N
Graduate School of Human Life Sciences, Osaka City University, Osaka, 558-8585, Japan. furusawa@life.osaka-cu.ac.jp
D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood*; D002645:Chickens; D005504:Food Analysis / Q000706:statistics & numerical data*; D005506:Food Contamination / Q000032:analysis*; D011200:Poultry; D012997:Solvents / Q000737:chemistry; D013422:Sulfamonomethoxine / Q000097:blood* / Q000378:metabolism
D000890:Anti-Infective Agents; D012997:Solvents; D013422:Sulfamonomethoxine