Aspirin induces the production of the inflammatory mediator 8-epi-PGF in mast cells.
Recently it has been shown that mast cells, through release of their pro-inflammatory mediators, are involved in aspirin attacks in aspirin-sensitive patients. To date, little information is available concerning 8-isoprostane (8-epi-prostaglandin F) production by mast cells. Therefore, we examined whether exposure of mast cells to aspirin can lead to isoprostane production. In this study we show that in mast cells, IgE and antigen stimulates an intracellular oxidative burst inducing H(2)O(2) and 8-epi-PGF production. Moreover, we show that exposure of mast cells to aspirin directly induces the production of 8-epi-PGF. Our study suggests that production of 8-epi-PGF by mast cells could contribute to the inflammatory response in e.g. aspirin-sensitive asthma patients.
Mortaz
Esmaeil
E
Department of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO BOX 80082, 3508 TB Utrecht, The Netherlands.
Redegeld
Frank A
FA
Nijkamp
Frans P
FP
Engels
Ferdi
F
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology*; D002478:Cells, Cultured; D004140:Dinitrophenols / Q000276:immunology; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011759:Pyrrolidines / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012709:Serum Albumin / Q000276:immunology; D013859:Thiocarbamates / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D004140:Dinitrophenols; D018836:Inflammation Mediators; D011759:Pyrrolidines; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D013859:Thiocarbamates; C058064:dinitrophenyl-human serum albumin conjugate; C020972:pyrrolidine dithiocarbamic acid; C075750:8-epi-prostaglandin F2alpha; D007073:Immunoglobulin E; D015237:Dinoprost; D006861:Hydrogen Peroxide; D001241:Aspirin