title: Proliferation of neural precursors in the subventricular zone after chemical lesions of the nigrostriatal pathway in rat brain.
abstract: The proliferative activity of neural precursors from the subventricular zone (SVZ) was investigated after a unilateral lesion was formed in the nigrostriatal pathway in adult rats. The lesion was formed by unilateral injection of 6-hydroxydopamine (6-OHDA) into the nigrostriatal pathway, and then bromodeoxyuridine (BrdU) was injected (ip) for 4 days or 2 weeks 10 days after the lesion was formed. The rats were killed, and the brain sections were immunohistochemically stained to detect the expression of BrdU, polysialylated neural-cell-adhesion molecule (PSA-NCAM), glial fibrillary acidic protein (GFAP) and tyrosine hydroxylase (TH) in the SVZ and the striatum (STR). The results showed that the BrdU(+) cells increased significantly in the SVZ, ipsilateral to the lesion at 2 weeks after the lesion. The PSA-NCAM(+) and GFAP(+) cells were also increased in the SVZ at this time. Some BrdU-labeled cells were seen in the same side of the STR and were double-labeled with PSA-NCAM. These cells had a tendency to migrate from the SVZ to the STR. The number of positive cells decreased at 4 weeks after the lesion was formed. The number of nigrostriatal projections with TH(+) decreased significantly in the STR on the lesion side, and the level of decrease was related to the quantity of BrdU-labeled cells at 2 weeks. These results indicate that the neural precursors in the SVZ of adult rats may increase after a lesion has been formed in the nigrostriatal pathway, and these cells might migrate into the STR on the same side.
authors.affiliation: Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
authors.forename: Bing Fang
authors.initials: BF
authors.lastname: Liu
authors.affiliation: Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
authors.forename: Er Jing
authors.initials: EJ
authors.lastname: Gao
authors.affiliation: Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
authors.forename: Xian Zhi
authors.initials: XZ
authors.lastname: Zeng
authors.affiliation: Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Ji
authors.affiliation: Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Beijing Institute of Neuroscience, Capital University of Medical Sciences, Beijing 100069, PR China. Electronic address: xuqy@cpums.edu.cn.
authors.forename: Qun Yuan
authors.initials: QY
authors.lastname: Xu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001973:Bromodeoxyuridine; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D003714:Denervation; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008297:Male; D009410:Nerve Degeneration / Q000139:chemically induced / Q000503:physiopathology; D009416:Nerve Regeneration / Q000502:physiology*; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D009434:Neural Pathways / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D009473:Neuronal Plasticity / Q000502:physiology*; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D012794:Sialic Acids / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013378:Substantia Nigra / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D039842:Neural Cell Adhesion Molecule L1; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D001973:Bromodeoxyuridine