title: Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea.
abstract: Like several other phytopathogenic fungi, the ascomycete Botrytis cinerea is known to produce the plant hormone abscisic acid (ABA) in axenic culture. Recently, bcaba1, the first fungal gene involved in ABA biosynthesis, was identified. Neighborhood analysis of bcaba1 revealed three further candidate genes of this pathway: a putative P450 monooxygenase-encoding gene (bcaba2), an open reading frame without significant similarities (bcaba3), and a gene probably coding for a short-chain dehydrogenase/reductase (bcaba4). Targeted inactivation of the genes proved the involvement of BcABA2 and BcABA3 in ABA biosynthesis and suggested a contribution of BcABA4. The close linkage of at least three ABA biosynthetic genes is strong evidence for the presence of an abscisic acid gene cluster in B. cinerea.
authors.affiliation: Westf. Wilhelms-Universität Münster, Institut für Botanik, Schlossgarten 3, 48149 Münster, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Siewers
authors.forename: Leonie
authors.initials: L
authors.lastname: Kokkelink
authors.forename: Jørn
authors.initials: J
authors.lastname: Smedsgaard
authors.forename: Paul
authors.initials: P
authors.lastname: Tudzynski
mesh_terms: D000040:Abscisic Acid / Q000096:biosynthesis* / Q000737:chemistry; D020171:Botrytis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D008969:Molecular Sequence Data; D005810:Multigene Family; D017422:Sequence Analysis, DNA
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D005656:Fungal Proteins; D000040:Abscisic Acid