title: [Analysis of fatty acids in Gmnocypris przewalskii oil by gas chromatography/mass spectrometry with base-catalyzed transesterification].
abstract: The composition of fatty acids (FA) in Gymnocypris przewalskii oil was identified and quantified by gas chromatography (GC)/electron impact (EI) mass spectrometry (MS). A base-catalyzed transesterification method was used to convert fatty acids to methyl esters. The lipids were extracted using petroleum ether and the total lipids in dried meat and skin of Gymnocypris przewalskii were about 25%. Forty-seven fatty acids were identified in the current study. Main types of fatty acids found in the oils were normal saturated, mono-branched, multi-branched, cyclopropane, furanoid, normal monounsaturated and polyunsaturated fatty acids. Saturated fatty acids were approximately 25. 7% of the total, and the main components were C(14:0) (3.4%), C(16:0) (19.4%) and C(18:0) (1.1%). Unsaturated fatty acids were totally 73.6%, and the major components of monounsaturated fatty acids were C(16:1 (9)) (19.8%), C(18:1) (9)) (18. 6%) and C(18:1 (11)) (7.3%); polyunsaturated fatty acids were mainly composed of C(18:2 (9,12)) (4.8%), C(18:3 (9, 12, 15)) (3.1%), C(20:4 (5, 8, 1, 14)) (1.2%), C(20:5 (5, 8, 11, 14, 17)) (EPA, 9.4%) and C(22:6 (4, 7, 10, 13, 16, 19)) (DHA, 6.7%). Especially, furyl-, cyclopropane- and several odd and branched chain fatty acids were found in Gymnocypris przewalskii oil. It is thus an important dietary resource of functional fatty acids.
authors.affiliation: Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 730000, China. bohb1212@163.com
authors.forename: Haibo
authors.initials: H
authors.lastname: Bo
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Zongde
authors.initials: Z
authors.lastname: Zhai
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Li
authors.forename: Liren
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D002347:Carps; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009821:Oils / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D009821:Oils