Expression and function of bactericidal/permeability-increasing protein in human genital tract epithelial cells.
Genital tract epithelia regularly encounter and adapt to the existence of bacterial pathogens. This study provides evidence that the endocervical and ectocervical epithelia of the human female genital tract express bactericidal/permeability-increasing protein (BPI). The constitutive expression of BPI was restricted to cell-bound protein and unaffected by human papillomavirus type 16/E6E7 immortalization and proinflammatory cytokine stimulation. Epithelial BPI was, in part, responsible for killing a commensal strain of Escherichia coli. The results of the present study suggest that BPI is tightly regulated and functionally expressed by epithelial cells in the female reproductive tract and may play a role in regulating bacterial colonization in the genital mucosa.
Canny
Geraldine O
GO
Center for Experimental Therapeutics and Reperfusion Injury, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Trifonova
Radiana T
RT
Kindelberger
David W
DW
Colgan
Sean P
SP
Fichorova
Raina N
RN
D023181:Antimicrobial Cationic Peptides / Q000378:metabolism* / Q000494:pharmacology; D001798:Blood Proteins / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000166:cytology*; D004847:Epithelial Cells / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism* / Q000494:pharmacology
D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D008565:Membrane Proteins; C021623:bactericidal permeability increasing protein