Decreased levels of insulin-like growth factor-1 and vascular endothelial growth factor relevant to the ossification disturbance in femoral heads spontaneous hypertensive rats.
Ossification disturbance in femoral head reportedly is seen in the Spontaneously Hypertensive rats (SHR) between ages of 10 and 20 weeks. We investigated serum and tissue levels of insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) in SHR relevant to the ossification disturbance and osteonecrosis of the femoral head. Serum levels of IGF-1 and VEGF were significantly lower in SHR than in Wistar Kyoto rats (WKY) at weeks 5, 10, 15 and 20 (p<0.005). The incidence of histological ossification disturbance of the femoral head was higher in SHR (59%) than in WKY (40%) at week 20. Lower serum and local levels of VEGF in SHR appeared to be related to the incomplete ossification of the femoral heads. Immunohistochemical study showed significantly lower numbers of IGF-1 and VEGF positive chondrocytes in the femoral epiphyseal cartilage of SHR than in those of WKY at weeks 10, 15 and 20. Our results suggest that local and/or systemic levels of IGF-1 and VEGF between ages of 5 and 20 weeks might play roles in the pathogenesis of ossifi cation disturbance of the femoral head in SHR.
Komiyama
Takamitsu
T
Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
Nishida
Keiichiro
K
Yorimitsu
Masanori
M
Doi
Hideyuki
H
Miyazawa
Shinichi
S
Kitamura
Ai
A
Yoshida
Aki
A
Nasu
Yoshihisa
Y
Abe
Nobuhiro
N
Ozaki
Toshifumi
T
D000818:Animals; D019902:Chondrocytes / Q000737:chemistry / Q000473:pathology; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005271:Femur Head Necrosis / Q000097:blood* / Q000503:physiopathology*; D006132:Growth Plate / Q000737:chemistry / Q000473:pathology; D006973:Hypertension / Q000503:physiopathology*; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D009999:Ossification, Heterotopic / Q000097:blood / Q000209:etiology / Q000473:pathology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I