title: Periodontopathic bacterial endotoxin-induced tumor necrosis factor alpha production was inhibited by exercise in mice.
abstract: The effects of exercise on serum interleukin-6 and tumor necrosis factor alpha levels were investigated using mice. Five-week-old female BALB/c mice (Th2-biased) and C57BL/6 mice (Th1-biased) were divided into exercise and control groups. The exercise group was exercised in a rotating basket type treadmill for 1 h (5 r.p.m.). Blood was collected and the serum was separated immediately after exercise. The serum interleukin-6 and tumor necrosis factor alpha levels were measured using an Endogen ELISA kit. Exercise significantly increased the serum interleukin-6 level in the two strains of mice (P<0.05 and P<0.01). The tumor necrosis factor alpha level was decreased in the exercise group. Next, periodontopathic bacterial endotoxin (lipopolysaccharide) was administered after exercise, and the effects of exercise on the lipopolysaccharide-induced serum interleukin-6 and tumor necrosis factor alpha levels were investigated. Exercise inhibited lipopolysaccharide-induced tumor necrosis factor alpha production, suggesting it has a defensive action against endotoxin shock.
authors.affiliation: Department of Microbiology, Oral Health Science Center, Tokyo Dental College, Chiba, Japan. tekato@tdc.ac.jp
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kato
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kaneko
authors.forename: Ryuta
authors.initials: R
authors.lastname: Kimizuka
authors.forename: Katsuji
authors.initials: K
authors.lastname: Okuda
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology; D000818:Animals; D005260:Female; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood* / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010805:Physical Conditioning, Animal / Q000502:physiology*; D016966:Porphyromonas gingivalis / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha