title: Formation of lambda-DNA's in parallel- and crossed-line arrays by molecular combing and scanning-probe lithography.
abstract: With the combination of a molecular combing technique and scanning-probe lithographic patterning, lambda-DNA's were stretched and aligned to form line array structures on patterned organic monolayer surfaces. The pattern was generated by anodizing a silicon surface using scanning-probe lithography to implant a polar organic layer in the middle of a nonpolar layer. The molecule in the polar layer, (aminopropyl)triethoxysilane (APS), has a -NH(3)(+) terminal group, which interacts strongly with phosphate backbone of DNA and provides a site for selective attachment of DNA. When parallel lines of APS were patterned, followed by combing along the lines, a single DNA was attached from the very top of each line and stretched along the line all the way to the bottom. The DNA-APS interaction was strong enough to withstand the second combing applied perpendicular to the first one. Thereby, the crossed-line array of DNA's was formed on the crossed-line array pattern of APS on a silicon substrate.
authors.affiliation: Department of Chemistry, Sungkyunkwan University, Suwon, Korea.
authors.forename: Minjung
authors.initials: M
authors.lastname: Shin
authors.forename: Chilwoo
authors.initials: C
authors.lastname: Kwon
authors.forename: Seong Kyu
authors.initials: SK
authors.lastname: Kim
authors.forename: Hyung Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Yonghan
authors.initials: Y
authors.lastname: Roh
authors.forename: Byungyou
authors.initials: B
authors.lastname: Hong
authors.forename: Jong Bae
authors.initials: JB
authors.lastname: Park
authors.forename: Haeseong
authors.initials: H
authors.lastname: Lee
mesh_terms: D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008859:Microscopy, Polarization; D036103:Nanotechnology; D009690:Nucleic Acid Conformation; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011437:Propylamines; D012821:Silanes; D004247:DNA; C477625:amino-propyl-triethoxysilane; D012825:Silicon