title: Localization of genetic elements of intact and derivative chromosome 11 and 22 territories in nuclei of Ewing sarcoma cells.
abstract: Recurring chromosomal abnormalities are associated with specific tumour types. The EWSR1 and FLI1 genes are involved in balanced translocation t(11;22)(q24;q12), which is present in more than 85% of Ewing sarcomas. In our previous study, we have found that the fusion genes pertaining to both derivative chromosomes 11 and 22 in Ewing sarcoma cell nuclei are shifted to the midway nuclear position between the native EWSR1 and FLI1 genes. In this contribution we focused our attention at nuclear positioning of other genetic elements of chromosomes 11 and 22 in order to find if the whole derivative chromosomes or only their translocated parts change their nuclear positions in comparison with the native chromosomes. Using repeated fluorescence in situ hybridization and high-resolution cytometry, 2D radial positions of EWSR1, BCR, FLI1, BCL1 genes and fluorescence weight centres of chromosome territories were compared for intact and derivative chromosomes 11 and 22 in nuclei of three Ewing sarcoma samples. Significant radial shift was obtained for the derivative EWSR1, FLI1 and BCL1 genes and for the derivative chromosome 11 compared with the intact ones and not very significant for chromosome 22 and the BCR gene. Our results also suggest that the mean nuclear positions of fusion genes are determined by the final structure of the derivative chromosomes and do not depend on the location of the translocation event.
authors.affiliation: Laboratory of Optical Microscopy, Faculty of Informatics, Masaryk University, Botanická 68a, 602 00 Brno, and Laboratory of Molecular Biology, Faculty Hospital Motol, Praha 5, Czech Republic.
authors.forename: Renata
authors.initials: R
authors.lastname: Taslerová
authors.forename: Stanislav
authors.initials: S
authors.lastname: Kozubek
authors.forename: Eva
authors.initials: E
authors.lastname: Bártová
authors.forename: Pavla
authors.initials: P
authors.lastname: Gajdusková
authors.forename: Roman
authors.initials: R
authors.lastname: Kodet
authors.forename: Michal
authors.initials: M
authors.lastname: Kozubek
mesh_terms: D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000502:physiology; D002467:Cell Nucleus / Q000235:genetics*; D048429:Cell Size; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11; D002892:Chromosomes, Human, Pair 22; D005260:Female; D018628:Gene Dosage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000166:cytology; D018213:Neoplasms, Bone Tissue / Q000235:genetics / Q000473:pathology; D011336:Probability; D051789:Proto-Oncogene Protein c-fli-1 / Q000235:genetics / Q000502:physiology; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D012512:Sarcoma, Ewing / Q000235:genetics* / Q000473:pathology; D012689:Sequence Homology, Nucleic Acid; D014178:Translocation, Genetic
chemical_list: D002148:Calmodulin-Binding Proteins; C500986:EWSR1 protein, human; C078433:FLI1 protein, human; D051789:Proto-Oncogene Protein c-fli-1; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins