title: Membrane-bound dimer structure of a beta-hairpin antimicrobial peptide from rotational-echo double-resonance solid-state NMR.
abstract: The intermolecular packing of a beta-hairpin antimicrobial peptide, PG-1, in lipid bilayers is determined using solid-state NMR distance measurements. Previous spin counting experiments showed that PG-1 associates as dimers in POPC bilayers; however, the detailed dimer structure was unknown. We have now measured several intermolecular 13C-19F, 1H-13C, and 15N-13C distances in site-specifically labeled PG-1 to constrain the structure of the intermolecular interface. The distances are measured using the rotational-echo double-resonance (REDOR) technique under magic-angle spinning. The results indicate that two PG-1 molecules align in a parallel fashion with the C-terminal strand of the hairpin forming the dimer interface. Six hydrogen bonds stabilize this interface, and the Phe12 side chain adopts the g- conformation in the membrane as in solution. The parallel packing of the peptide in the lipid bilayer differs from the antiparallel dimer found in DPC micelles and may be stabilized by its strong amphipathic character, which should facilitate its insertion into the amphipathic lipid bilayer. This study demonstrates the utility of the REDOR NMR technique for the elucidation of the oligomeric structure of membrane proteins.
authors.affiliation: Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: R
authors.initials: R
authors.lastname: Mani
authors.forename: M
authors.initials: M
authors.lastname: Tang
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.forename: J J
authors.initials: JJ
authors.lastname: Buffy
authors.forename: A J
authors.initials: AJ
authors.lastname: Waring
authors.forename: M A
authors.initials: MA
authors.lastname: Sherman
authors.forename: M
authors.initials: M
authors.lastname: Hong
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D019281:Dimerization; D006860:Hydrogen Bonding; D008051:Lipid Bilayers / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*
chemical_list: D023181:Antimicrobial Cationic Peptides; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D011506:Proteins; C094880:protegrin-1; C028694:1-palmitoyl-2-oleoylphosphatidylcholine