Amplification of the hyphal wall protein 1 gene to distinguish Candida albicans from Candida dubliniensis.
The authors developed a new, simple, and reliable PCR/restriction fragment length polymorphism technique, using amplification of the hyphal wall protein 1 gene of Candida albicans and its gene homologue in Candida dubliniensis, to differentiate the two species of Candida. Performed with a new primer set, CRR-f/CRR-r, PCR produced two different fragments: one of 1,180 bp for C. albicans, and one of 930 bp for C. dubliniensis.
Romeo
Orazio
O
Dipartimento di Scienze Microbiologiche, Genetiche e Molecolari, Università degli studi di Messina, Salita Sperone, 31 98166 Messina, Italy.
Racco
Cosimo
C
Criseo
Giuseppe
G
D002175:Candida / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers; D005656:Fungal Proteins / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D009172:Mycology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D017931:DNA Primers; D005656:Fungal Proteins; C118102:HWP1 protein, Candida albicans; D008562:Membrane Glycoproteins