title: DNA-binding properties of the antibody specific for the Dewar photoproduct of thymidylyl-(3-5')-thymidine.
abstract: A monoclonal antibody (DEM-1) specific for the Dewar photoproduct is used for detection and quantification of photolesions in DNA. To help understand the molecular recognition of damaged DNA by the antibody protein, we have cloned and sequenced the variable region genes of DEM-1. We have also prepared Fab fragments of DEM-1 (DEM1Fab), and synthesized two kinds of 3'-biotinylated oligonucleotides of different lengths containing a central Dewar photoproduct of TpT to analyze the effects of the antigen size on the binding rates by means of surface plasmon resonance (SPR). Results obtained from SPR analyses suggest that DEM1Fab may recognize tetranucleotide unit as the epitope.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo, Japan. morioka@pahrm.hokudai.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Morioka
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kurihara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Kousuke
authors.initials: K
authors.lastname: Satou
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Komatsu
authors.forename: Makiyo
authors.initials: M
authors.lastname: Uchida
authors.forename: Eiko
authors.initials: E
authors.lastname: Ohtsuka
authors.forename: Takuya
authors.initials: T
authors.lastname: Torizawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
authors.forename: Ichio
authors.initials: I
authors.lastname: Shimada
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Osamu
authors.initials: O
authors.lastname: Nikaido
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D004247:DNA / Q000737:chemistry* / Q000276:immunology* / Q000528:radiation effects; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010777:Photochemistry; D013936:Thymidine / Q000031:analogs & derivatives / Q000737:chemistry / Q000276:immunology* / Q000528:radiation effects*
chemical_list: D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D004247:DNA; D013936:Thymidine