title: Change in annexin A3 expression by regulatory factors of hepatocyte growth in primary cultured rat hepatocytes.
abstract: We have recently reported that annexin (Anx) A3 expression is necessary for hepatocyte growth in cultured rat hepatocytes seeded at half the subconfluent density on collagen. In the present study, we investigated the effects of various regulatory factors of hepatocyte growth on AnxA3 expression. AnxA3 expression was significantly reduced in hepatocytes cultured under various growth inhibitory conditions such as presence of dexamethasone, culture at subconfluent cell density, and on EHS-Matrigel and lactose-carrying styrene polymer. On the other hand, hepatocyte growth factor and epidermal growth factor, stimulators of hepatocyte growth, significantly increased AnxA3 expression in hepatocytes cultured on EHS-Matrigel. These results show close correlation between known stimulatory or inhibitory actions of various factors to hepatocyte growth and increase or decrease in AnxA3 expression, and suggest the involvement of AnxA3 in their regulation of hepatocyte growth.
authors.affiliation: Department of Nutrition and Physiology, Nihon University College of Bioresource Sciences, Fujisawa, Japan.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Harashima
authors.forename: Shingo
authors.initials: S
authors.lastname: Niimi
authors.forename: Hitomi
authors.initials: H
authors.lastname: Koyanagi
authors.forename: Masashi
authors.initials: M
authors.lastname: Hyuga
authors.forename: Seiji
authors.initials: S
authors.lastname: Noma
authors.forename: Taiichiro
authors.initials: T
authors.lastname: Seki
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Ariga
authors.forename: Toru
authors.initials: T
authors.lastname: Kawanishi
authors.forename: Takao
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D000818:Animals; D017318:Annexin A3 / Q000235:genetics*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D017931:DNA Primers; D004815:Epidermal Growth Factor / Q000494:pharmacology; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D017318:Annexin A3