Altered expression of DNA double-strand repair genes Ku70 and Ku80 in carcinomas of the oral cavity.
The relevance of double-strand DNA repair genes has been demonstrated in several tumours. The main aim of this study was to analyse the expression of the heterodimers Ku70 and Ku80, building regulatory subunits of the DNA-dependent protein kinase in 40 oral carcinomas. Ku70 expression was found in 87.5% of grade 1 and grade 3 tumours and in 82.9% of grade 2 carcinomas. Ku80 presence was noted in 87.5% of grade 1 tumours, 82.9% of grade 2 tumours and in all grade 3 tumours. Ku70-positive cells were present in 90.5% of tumours without and in 80% of tumours with lymphatic metastases. A similar relationship was found for Ku80 expression. Additionally, the expression of Ku70 was highly significantly related to smoking habits. Our results demonstrated that defects of DNA double-strand repair genes play an important role in the tumour progression of oral carcinomas.
Korabiowska
Monika
M
Institute of Pathology, Reinhard-Nieter Hospital, Academic Hospital of the University Göttingen, Wilhelmshaven, Germany. korabmon@yahoo.de
Voltmann
Jann
J
Hönig
Johannes F
JF
Bortkiewicz
Pawel
P
König
Frank
F
Cordon-Cardo
Carlos
C
Jenckel
Frederike
F
Ambrosch
Petra
P
Fischer
Gösta
G
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D034961:Antigens, Nuclear / Q000096:biosynthesis / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004260:DNA Repair / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D006801:Humans; D000072200:Ku Autoantigen; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009367:Neoplasm Staging; D012907:Smoking / Q000235:genetics / Q000378:metabolism
D034961:Antigens, Nuclear; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen