The neuroprotective effect of heme oxygenase (HO) on oxidative stress in HO-1 siRNA-transfected HT22 cells.
To investigate the role of heme oxygenase (HO) isozymes, we used siRNA technology to suppress HO-1 expression. HO-1 siRNA-transfected HT22 cells were vulnerable to hydrogen peroxide- and 4-hydroxynonenal-induced cytotoxicity. Biliverdin and bilirubin, degradative products of heme catalyzed by HO, protected HT22 cells from the insult of these oxidative stressors. These results suggest that inducible HO-1 plays a protective role against oxidative stress in HT22 cells.
Kaizaki
Asuka
A
Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.
Tanaka
Sachiko
S
Ishige
Kumiko
K
Numazawa
Satoshi
S
Yoshida
Takemi
T
D000447:Aldehydes / Q000633:toxicity; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000503:physiopathology; D002461:Cell Line, Transformed; D015536:Down-Regulation / Q000235:genetics; D020868:Gene Silencing / Q000502:physiology; D051547:Heme Oxygenase-1 / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000633:toxicity; D051379:Mice; D019636:Neurodegenerative Diseases / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology; D018696:Neuroprotective Agents / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics*; D034622:RNA Interference
D000447:Aldehydes; D018696:Neuroprotective Agents; D006861:Hydrogen Peroxide; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; C027576:4-hydroxy-2-nonenal