CD8 alpha alpha-mediated intraepithelial lymphocyte snatching of thymic leukemia MHC class Ib molecules in vitro and in vivo.
Thymic leukemia (TL) is a MHC class Ib molecule that interacts with CD8alphaalpha homodimers. CD8alphaalpha is abundantly expressed by intraepithelial T lymphocytes (IELs) located in close proximity to TL-expressing intestinal epithelial cells. In this study, we show that CD8alphaalpha(+) IELs "snatch" TL from the plasma membrane of TL-expressing cells and express TL in its proper orientation on their own cell surface. TL snatching is enhanced by cross-linking of IEL TCRs in a phosphatidylinositol kinase-dependent manner, and results in overall alterations to the IEL cell surface detected by enhanced binding of peanut agglutinin lectin. Induction of bowel inflammation results in the presence of TL on IELs, probably via in vivo snatching, providing the initial evidence for the interaction of CD8alphaalpha IELs with intestinal cells.
Pardigon
Nathalie
N
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA.
Takeda
Kazuyo
K
Saunier
Bertrand
B
Hornung
Felicita
F
Gibbs
James
J
Weisberg
Andrea
A
Contractor
Nikhat
N
Kelsall
Brian
B
Bennink
Jack R
JR
Yewdell
Jonathan W
JW
D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism*; D001693:Biological Transport, Active / Q000235:genetics / Q000276:immunology; D016827:CD8 Antigens / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D002450:Cell Communication / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000276:immunology* / Q000378:metabolism; D006183:H-2 Antigens / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D014162:Transfection
D000951:Antigens, Neoplasm; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D008562:Membrane Glycoproteins; C028388:thymus-leukemia antigens