title: Chronic resistance training in women potentiates growth hormone in vivo bioactivity: characterization of molecular mass variants.
abstract: This investigation determined the influence of acute and chronic resistance exercise on responses of growth hormone (GH) molecular variants in women. Seventy-four healthy young women (23 +/- 3 yr, 167 +/- 7 cm, 63.8 +/- 9.3 kg, 26.3 +/- 4.0% body fat) performed an acute bout of resistance exercise (6 sets of 10 repetition maximum squat). Blood samples were obtained pre- and postexercise. Resulting plasma was fractionated by molecular mass (fraction A, >60 kDa; fraction B, 30-60 kDa; and fraction C, <30 kDa) using chromatography. Fractionated and unfractionated (UF) plasma was then assayed for GH using three different detection systems (monoclonal immunoassay, polyclonal immunoassay, and rat tibial line in vivo bioassay). Subjects were then matched and randomly placed into one of four resistance exercise training groups or a control group for 24 wk. All experimental procedures were repeated on completion of the 24-wk resistance training programs. After acute exercise, immunoassays showed consistent increases in UF GH samples and fractions B and C; increases in fraction A using immunoassay were seen only in the monoclonal assay. No consistent changes in bioactive GH were found following acute exercise. Conversely, chronic exercise induced no consistent changes in immunoassayable GH of various molecular masses, whereas, in general, bioassayable GH increased. In summary, although acute exercise increased only immunoactive GH, chronic physical training increased the biological activity of circulating GH molecular variants. Increased bioactive GH was observed across all fractions and training regimens, suggesting that chronic resistance exercise increased a spectrum of GH molecules that may be necessary for the multitude of somatogenic and metabolic actions of GH.
authors.affiliation: Human Performance Laboratory, Dept. of Kinesiology, University of Connecticut, Storrs, CT 06269, USA. William.Kraemer@uconn.edu
authors.forename: William J
authors.initials: WJ
authors.lastname: Kraemer
authors.forename: Bradley C
authors.initials: BC
authors.lastname: Nindl
authors.forename: James O
authors.initials: JO
authors.lastname: Marx
authors.forename: Lincoln A
authors.initials: LA
authors.lastname: Gotshalk
authors.forename: Jill A
authors.initials: JA
authors.lastname: Bush
authors.forename: Jill R
authors.initials: JR
authors.lastname: Welsch
authors.forename: Jeff S
authors.initials: JS
authors.lastname: Volek
authors.forename: Barry A
authors.initials: BA
authors.lastname: Spiering
authors.forename: Carl M
authors.initials: CM
authors.lastname: Maresh
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Mastro
authors.forename: Wesley C
authors.initials: WC
authors.lastname: Hymer
mesh_terms: D000328:Adult; D000818:Animals; D015444:Exercise / Q000502:physiology*; D005260:Female; D013006:Growth Hormone / Q000096:biosynthesis* / Q000097:blood / Q000737:chemistry; D006801:Humans; D007016:Hypophysectomy; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D014891:Weight Lifting / Q000502:physiology*
chemical_list: D013006:Growth Hormone