HPLC determination of anethole trithione and its application to pharmacokinetics in rabbits.
To evaluate the relative bioavailability of anethole trithione (ATT) from self-microemulsifying drug delivery system (SMEDDS) and tablet, a sensitive, accurate and reliable liquid chromatography method was developed and validated to determine ATT in rabbit plasma. Chromatographic separation was performed on a Diamonsil C18 column by using a mixture of methanol-water (90:10, v/v) delivered at a flow rate of 1.0 ml/min. The wavelength was set at 348 nm and mifepristone was used as the internal standard. A linear relationship for ATT was found in the range of 0.5-32 ng/ml. The mean extraction recoveries of ATT determined over three concentrations were 84.7+/-5.8, 92.3+/-3.4 and 89.9+/-5.1%. After administration of SMEDDS and tablets to rabbits, significant differences were found in main pharmacokinetic parameters of Tmax, Cmax and AUC(0-infinity) between these two formulations, and a 2.5-fold enhancement of relative bioavailability of ATT was observed from the SMEDDS compared with tablets.
Jing
Qiufang
Q
Department of Pharmaceutical Engineering, School of Chemistry and Pharmaceutics, East China University of Science and Technology, Shanghai 200237, China.
Shen
Yongjia
Y
Ren
Fuzheng
F
Chen
Jianming
J
Jiang
Zhiting
Z
Peng
Baoliang
B
Leng
Yixin
Y
Dong
Jing
J
D000781:Anethole Trithione / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D016503:Drug Delivery Systems; D004655:Emulsions; D008297:Male; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets
D004655:Emulsions; D013607:Tablets; D000781:Anethole Trithione