title: [Human large intestine adenocarcinoma cells (CaCo-2) in the process of cultivation].
abstract: Using cytomorphometry and cytophotometry cells of human large intestine adenocarcinoma (CaCo-2) were studied under condition of a 10 day cultivation. A reverse dependence was established between proliferative activity and monolayer density. The increase of the latter inhibits proliferation and promotes the formation of islets of polymorph cells. 2c-cells could be seen only at the beginning of culture growth; a larger part of cells polyploidized by cell blocking in G2-phase. These cells do not divide, which is testified by the absence of 2c-cells, but some part of 4c-cells start the next cycle, accumulates 8c-DNA and then divides, replenishing the 4c-cells population. In the process of cultivation, we observed an increase in the number and total volume of nucleoli in the nuclei, and a rise in DNA amount in the peri-nucleolar chromatin. The formation of numerous 4c-cells with multi-nucleolar nuclei may define an increase of functional activity of CaCo-2 culture as the whole, whereas the formation of separated groups of such cells in the monolayer may denote a possible initiation of their differentiation.
authors.forename: E M
authors.initials: EM
authors.lastname: Karalova
authors.forename: L O
authors.initials: LO
authors.lastname: Abroian
authors.forename: L A
authors.initials: LA
authors.lastname: Akopian
authors.forename: M G
authors.initials: MG
authors.lastname: Gasparian
authors.forename: Z A
authors.initials: ZA
authors.lastname: Karalian
authors.forename: N G
authors.initials: NG
authors.lastname: Dzhagatspanian
authors.forename: Z R
authors.initials: ZR
authors.lastname: Ter-Pogosian
authors.forename: L A
authors.initials: LA
authors.lastname: Kamalian
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Magakian
mesh_terms: D018938:Caco-2 Cells / Q000166:cytology*; D002452:Cell Count; D018929:Cell Culture Techniques; D002455:Cell Division; D002466:Cell Nucleolus / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics; D006801:Humans; D019044:Image Cytometry; D011123:Polyploidy; D013997:Time Factors