Pharmacological differences between Liu-Jun-Zi-Tang, a traditional Chinese herbal medicine, and domperidone on isolated guinea-pig ileum.
The different characteristics of Liu-Jun-Zi-Tang, a spray-dried powdered extract from 8 Chinese herbs, and domperidone, a D2 antagonist, both of which are used clinically for the treatment of gastritis, were validated in isolated guinea pig ileal longitudinal muscle. Liu-Jun-Zi-Tang at 0.05 mg/ml did not affect the twitch response by electrical field stimulation, but it concentration-dependently inhibited the twitch response at concentrations of 0.1 and 1.0 mg/ml, and maximum inhibition occurred 5 min after application of Liu-Jun-Zi-Tang. In the presence of Liu-Jun-Zi-Tang, the concentration-response curve for ACh-contraction showed inhibition in a non-competitive manner, which is the same as domperidone. In the presence of Liu-Jun-Zi-Tang (1.0 mg/ml), ACh (10(-7) M)-, histamine (5x10(-7) M)- and barium chloride (10(-4) M)-induced basal contractions were inhibited by 24, 19 and 54 %, respectively. On the other hand, in the presence of domperidone (2x10(-5) M), ACh- and barium chloride-induced basal contractions were inhibited by 28 and 63%, respectively, similar to that in the presence of Liu-Jun-Zi-Tang. However, the inhibition of histamine-induced contraction in the presence of domperidone was significantly increased (81%) compared with that in the presence of Liu-Jun-Zi-Tang. These findings suggest that Liu-Jun-Zi-Tang has milder effects on histamine-induced disorders than domperidone. In other words, domperidone has a more potent effect on histamine-induced disorders.
Ozaki
Masanobu
M
Department of Toxicology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Japan. ozaki@niigata-pharm.ac.jp
Nagatomo
Takafumi
T
Maeda
Takehiko
T
Kishioka
Shiroh
S
Yamamoto
Hiroyuki
H
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D004294:Domperidone / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004558:Electric Stimulation; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D004294:Domperidone; D000109:Acetylcholine