title: Streptokordin, a new cytotoxic compound of the methylpyridine class from a marine-derived Streptomyces sp. KORDI-3238.
abstract: A new cytotoxic compound, streptokordin, and four known compounds, nonactic acid, dilactone, trilactone, and nonactin, were isolated from the fermentation broth of a marine actinomycete strain collected in deep-sea sediments. Biochemical tests and 16S rDNA analysis indicated that the strain belongs to the genus Streptomyces. This actinomycete produces various bioactive secondary metabolites. Fractionations by solvent partitioning, silica vacuum flash chromatography, and reversed-phase HPLC gave a pure cytotoxic compound, designated streptokordin. Its structure was elucidated by FAB-MS, 1H, 13C, and 2D NMR spectroscopy. Streptokordin exhibited significant cytotoxicity against seven human cancer cell lines but showed no growth inhibition against various microorganisms including bacteria and fungi.
authors.affiliation: Marine Natural Products Laboratory, Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600, Korea.
authors.forename: Seong-Yun
authors.initials: SY
authors.lastname: Jeong
authors.forename: Hee Jae
authors.initials: HJ
authors.lastname: Shin
authors.forename: Tae Sik
authors.initials: TS
authors.lastname: Kim
authors.forename: Hyi-Seung
authors.initials: HS
authors.lastname: Lee
authors.forename: Song-kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Hwan Mook
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D002845:Chromatography; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004354:Drug Screening Assays, Antitumor; D005658:Fungi / Q000187:drug effects; D019015:Geologic Sediments / Q000382:microbiology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D010802:Phylogeny; D011728:Pyridones / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D014871:Water Microbiology
chemical_list: D000903:Antibiotics, Antineoplastic; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011728:Pyridones; D012336:RNA, Ribosomal, 16S; C512209:streptokordin