[Effects of nitric oxide synthases on kidney in early phase of endotoxic shock in macaque].
OBJECTIVE
To study the pathogenesis of kidney injury in early phase of endotoxic shock.


METHODS
Eleven macaques were anesthetized and randomly divided into two groups: an experimental group (n=6), receiving a dose of 2.8 mg/kg lipopolysaccharides (LPS) by intravenous injection, and a control group (n=5), injected with normal saline of 1 ml/kg. The animals were sacrificed 120 minutes following endotoxin injection. With immunohistochemical technique endothelium nitric oxide synthases (eNOS) and inducible nitric oxide synthases (iNOS) protein of the renal tissue were determined. The ultrastructure was studied with electron microscope. Acid phosphatase (ACP) was also assayed.


RESULTS
One hundred and twenty minutes after LPS challenge, damages to the glomerulus and renal tubules were found, and lysosomes were increased in the renal cells. iNOS was positively expressed in vascular endothelium of glomerulus and epithelium of renal tubules in the experimental group, but not in the control group. eNOS was positively expressed in vascular endothelium of glomerulus; and peri-vascular region in both groups.


CONCLUSIONS
The present findings indicate that NO contributed to the pathogenesis of renal injury in the early phase of endotoxic shock in primates.
Ma
Ming
M
Department of Medicine, The Second Hospital of Nanjing, Medical College, South East University, Nanjing 210003, Jiangsu, China. maming_1971@yahoo.com.cn
Zhu
Xiao-yun
XY
Wang
Li-rong
LR
Gu
Xiao-jun
XJ
Yin
Guo-qing
GQ
D000818:Animals; D004195:Disease Models, Animal; D007668:Kidney / Q000201:enzymology / Q000473:pathology*; D008070:Lipopolysaccharides / Q000633:toxicity; D008251:Macaca; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D011897:Random Allocation; D012772:Shock, Septic / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology*
D008070:Lipopolysaccharides; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III