Expression profiling of human epidermal keratinocyte response following 1-minute JP-8 exposure.
The cDNA microarray analysis of 9600 expressed sequence tags was performed to examine the gene expression changes in human epidermal keratinocytes after 1-minute JP-8 exposure; 151 genes were identified as JP-8 responsive and classified into 8 clusters by self organization map. Genes involved in basal transcription and translations were up-regulated, whereas genes related to DNA repair, metabolism, and keratin were mostly down-regulated. Genes encoded for growth factors, apoptosis, signal transduction, and adhesion were also altered. These results indicated that human keratinocyte responds to a single dose of JP-8 insult and revealed several cellular processes previously not associated with jet fuel exposure.
Chou
Chi-Chung
CC
Department of Veterinary Medicine and Veterinary Medicine Teaching Hospital, National Chung-Hsing University, Taichung, Taiwan, ROC. ccchou@nchu.edu.tw
Yang
Jen-Hung
JH
Chen
San-Duo
SD
Monteiro-Riviere
Nancy A
NA
Li
Han-Ni
HN
Chen
Jeremy J W
JJ
D000328:Adult; D017209:Apoptosis; D002448:Cell Adhesion; D003124:Colorimetry; D004260:DNA Repair; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006801:Humans; D006838:Hydrocarbons / Q000494:pharmacology*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D015398:Signal Transduction; D012867:Skin / Q000187:drug effects* / Q000378:metabolism
D006838:Hydrocarbons; C098607:JP8 aviation fuel