Cell suspension cultures of Populus tremula x P. tremuloides exhibit a high level of cellulose synthase gene expression that coincides with increased in vitro cellulose synthase activity.
Compared to wood, cell suspension cultures provide convenient model systems to study many different cellular processes in plants. Here we have established cell suspension cultures of Populus tremula L. x P. tremuloides Michx. and characterized them by determining the enzymatic activities and/or mRNA expression levels of selected cell wall-specific proteins at the different stages of growth. While enzymes and proteins typically associated with primary cell wall synthesis and expansion were detected in the exponential growth phase of the cultures, the late stationary phase showed high expression of the secondary-cell-wall-associated cellulose synthase genes. Interestingly, detergent extracts of membranes from aging cell suspension cultures exhibited high levels of in vitro cellulose synthesis. The estimated ratio of cellulose to callose was as high as 50 : 50, as opposed to the ratio of 30 : 70 so far achieved with membrane preparations extracted from other systems. The increased cellulose synthase activity was also evidenced by higher levels of Calcofluor white binding in the cell material from the stationary-phase cultures. The ease of handling cell suspension cultures and the improved capacity for in vitro cellulose synthesis suggest that these cultures offer a new basis for studying the mechanism of cellulose biosynthesis.
Ohlsson
Anna B
AB
School of Biotechnology, Royal Institute of Technology, AlbaNova University Centre, Stockholm, Sweden.
Djerbi
Soraya
S
Winzell
Anders
A
Bessueille
Laurence
L
Ståldal
Veronika
V
Li
Xinguo
X
Blomqvist
Kristina
K
Bulone
Vincent
V
Teeri
Tuula T
TT
Berglund
Torkel
T
D001557:Benzenesulfonates / Q000378:metabolism; D002478:Cells, Cultured; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006824:Hybridization, Genetic; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D032107:Populus / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047071:beta-Glucans / Q000378:metabolism
D001557:Benzenesulfonates; D010940:Plant Proteins; D012333:RNA, Messenger; D047071:beta-Glucans; C095825:expansin protein, plant; C007061:C.I. Fluorescent Brightening Agent 28; D002482:Cellulose; D005964:Glucosyltransferases; C478648:cellulose synthase; D002480:Cellulase