title: MIB-1 labelling indices according to clinico-pathological variables in canine mammary tumours: a multivariate study.
abstract: The relationship between MIB-1 labelling indices (LI), as detected by immunohistochemical methods, and other clinico-pathological characteristics was studied in a series of 77 malignant mammary tumours surgically removed from 47 female dogs. The immunostaining was assessed on the basis of the estimated percentage of positive cells in the areas of highest labelling. Multivariate logistic regression demonstrated no influence of breed, age, previous pregnancies, previous progestin administration, histological type or location of the tumour on MIB-1 LI. MIB-1 LI was significantly related to the size of the tumour, necrosis, invasive growth and histological grade, but not with ulceration, lymph node metastasis, skin fixation or E-cadherin expression. The significant relationship between MIB-1 LI and other known factors of poor prognosis suggests that a high LI may have prognostic value in canine malignant mammary tumours.
authors.affiliation: Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), University of Porto, Largo Professor Abel Salazar, 2, 4099-003, Porto, Portugal.
authors.forename: Augusto José Ferreira
authors.initials: AJ
authors.lastname: De Matos
authors.forename: Célia Cristina Carreiras
authors.initials: CC
authors.lastname: Lopes
authors.forename: Augusto Manuel Rodrigues
authors.initials: AM
authors.lastname: Faustino
authors.forename: Júlio Gil Vale
authors.initials: JG
authors.lastname: Carvalheira
authors.forename: Marta Susana Amaro
authors.initials: MS
authors.lastname: Dos Santos
authors.forename: Gerard Röels
authors.initials: GR
authors.lastname: Rutteman
authors.forename: Maria de Fátima Rodrigues Moutinho
authors.initials: Mde F
authors.lastname: Gärtner
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D016015:Logistic Models; D008207:Lymphatic Metastasis; D015674:Mammary Neoplasms, Animal / Q000378:metabolism / Q000473:pathology*; D015999:Multivariate Analysis; D009367:Neoplasm Staging
chemical_list: D019394:Ki-67 Antigen