[Effect of silencing of hyaluronidase gene HYAL1 by RNA interference on proliferation of human breast cancer cells].
OBJECTIVE
It is reported that hyaluronidase is related to malignant potentiality of human breast cancer. This study was to investigate whether HYAL1 RNA interference (RNAi) could effectively inhibit gene mRNA expression as well as cell proliferation in human breast cancer cells.


METHODS
Chemically synthesized double stranded RNA (dsRNA) targeting HYAL1 was transfected into human breast cancer cell lines MDA-MB-231, MDA-MB-453S, ZR-75 and ZR-75-30 using SiPORT lipid. The transfection efficiency was observed under a fluorescence confocal microscopy. Expression of HYAL1 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Cell proliferation and cell cycle were determined by MTT and flow cytometry assay, respectively.


RESULTS
HYAL1 siRNA effectively inhibited HYAL1 mRNA expression (P<0.05), cell proliferation (P<0.05), and induced cell cycle arrest in G0/G1 phase with a significant decrease of cells in S-phase(P<0.05).


CONCLUSIONS
HYAL1-siRNA may be used as a new approach in human breast cancer gene therapy.
Tan
Jin-Xiang
JX
Department of General Surgery, The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, P. R. China.
Ren
Guo-Sheng
GS
Tu
Gang
G
Li
Xiao-Tao
XT
Wang
Xiao-Yi
XY
Ran
Liang
L
D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000096:biosynthesis* / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D014162:Transfection
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D006821:Hyaluronoglucosaminidase