[Persistence of the Salmonella enteritidis recombinant strain with expressed HBc antigen in mice and macrophage culture].
The behavior of S. enteritidis recombinant strain E-23/pKDNA(3.1) with HBc antigen expressed by the eukaryotic promoter (DNA vaccine) in the body of mice after their infection per rectum and in the culture of macrophages was studied. The expression of HBc was shown to lead to the loss of the capacity of salmonellae for persistence in parenteral lymphoid tissue and for inducing the formation of anti-HBc antibodies. The study of the interaction of salmonellae with the macrophage culture revealed that the synthesis of HBc antigen inhibited the intracellular multiplication of salmonellae.
Donin
M V
MV
Boĭchenko
M N
MN
Vorob'ev
A A
AA
D000285:Administration, Rectal; D000818:Animals; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D006509:Hepatitis B / Q000517:prevention & control; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008643:Mesentery / Q000276:immunology; D051379:Mice; D009000:Monocytes / Q000382:microbiology*; D010587:Phagocytosis; D012477:Salmonella enteritidis / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D013154:Spleen / Q000382:microbiology; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000008:administration & dosage; D014761:Viral Hepatitis Vaccines / Q000008:administration & dosage
D006512:Hepatitis B Core Antigens; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines