Bacterial derived proteoliposome as ideal delivery system and cellular adjuvant.
We explored the potential of a proteoliposome (PL) from the outer membrane of N. meningitidis B, as an immunopotentiator and as a vector for antigen delivery to dendritic cells (DC). DC were incubated with PL resulting in up-regulation of MHC-II, CD40, CD80, and CD86 expression and production of TNFalpha and IL12(p70). Ovoalbumin (OVA) was incorporated within PL (PL-OVA). PL-OVA presented OVA-specific peptides to CD4+ and CD8+ OVA-specific T-cell hybridomas. PL exerts an immunomodulatory effect on DC and is a general system to deliver antigens for presentation to CD4+ and CD8+ T-cells possibly implicated in the induction CD8+ cytotoxic T lymphocytes (CTLs) responses.
Rodríguez
Tamara
T
Department of Immunology, Finlay Institute, 27 Ave, b/198 and 202 La Lisa, P.O. Box 16017, Havana, Cuba. trodriguez@finlay.edu.cu
Pérez
Oliver a
Oa
Ugrinovic
Sanja
S
Bracho
Gustavo
G
Mastroeni
Pietro
P
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D017951:Antigen Presentation; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D016503:Drug Delivery Systems; D051379:Mice; D009345:Neisseria meningitidis / Q000276:immunology; D011510:Proteolipids
D000276:Adjuvants, Immunologic; D000941:Antigens; D001425:Bacterial Outer Membrane Proteins; D011510:Proteolipids; C020485:proteoliposomes