Interleukin-2 reconstitutes defective human immunodeficiency virus (HIV), and cytomegalovirus (CMV) specific CD8+ T cell proliferation in HIV infection.
Recent studies indicate that a defective proliferative response of HIV-specific CD8+ T cells is associated with the lack of virologic control in chronic HIV infection in humans. The possible mechanisms that might be responsible for the reduced proliferative potential of HIV-specific CD8+ T cells and conditions conducive to the proliferation of CD8+ T cells were examined in 14 HIV-infected individuals and 7 HIV-uninfected controls using CFSE labeling and flow cytometry techniques, and analyzed data using 2 quantitative measurements: the percentages of proliferating CD8+ T cells (Tp), and the maximum number of cell divisions (Dm) after stimulation. It was found that CD8+ T cells from HIV-infected and -uninfected subjects proliferated equally well after polyclonal stimulation by phylohemagglutinin A (PHA); both groups reached a Tp of 92%-96% and a Dm of 5-8. However, in HIV-infected subjects, proliferation of HIV- and CMV-specific CD8+ T cells was significantly reduced compared to proliferation of CMV- specific CD8+ T cells from HIV-uninfected subjects. These defective proliferative responses of HIV- and CMV-specific CD8+ T cells were restored by the addition of IL-2 at the time of stimulation. These results may have implications for the design of immune modulation strategies in vivo.
Yu
Jie
J
Department of Medicine, Infectious Diseases Unit, University of Rochester School of Medicine and Dentistry, New York 14642, USA.
Chen
Huiyuan
H
Horton
Helen
H
Bansal
Anju
A
McElrath
Julie M
JM
Reichman
Richard
R
Goepfert
Paul
P
Jin
Xia
X
D000595:Amino Acid Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002455:Cell Division; D002478:Cells, Cultured; D002908:Chronic Disease; D003587:Cytomegalovirus / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000235:genetics; D005434:Flow Cytometry; D005452:Fluoresceins; D016655:HIV Core Protein p24 / Q000235:genetics; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007376:Interleukin-2 / Q000276:immunology*; D007963:Leukocytes, Mononuclear; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D010835:Phytohemagglutinins / Q000276:immunology; D013194:Staining and Labeling; D013388:Succinimides; D037182:T-Cell Antigen Receptor Specificity; D014481:United States
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D018984:Epitopes, T-Lymphocyte; D005452:Fluoresceins; D016655:HIV Core Protein p24; D007376:Interleukin-2; D010455:Peptides; D010835:Phytohemagglutinins; D013388:Succinimides