Simple synthesis of a building block for solid-phase labeling of oligonucleotides with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA).
Synthesis of a nucleosidic phosphoramidite building block which allows solid-phase introduction of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) to synthetic oligonucleotides using a standard oligonucleotide synthesizer is described.
Jaakkola
Lassi
L
PerkinElmer Life and Analytical Sciences, Turku Site, POB 10, FIN-20101 Turku, Finland.
Ylikoski
Alice
A
Hovinen
Jari
J
D001483:Base Sequence; D002853:Chromatography, Liquid; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D006573:Heterocyclic Compounds, 1-Ring; D009841:Oligonucleotides; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid