[Effects of deltamethrin on permeability of mitochondrial membrane and expression of cytochrome C in brain tissue of rats].
OBJECTIVE
To study the effects of deltamethrin (DM) on the permeability of mitochondrial membrane and the expression of cytochrome C in brain tissue of rats.


METHODS
Wistar rats were randomizedly divided into five groups (including four treated groups and one control group). In the treated groups, DM of 12.5 mg/kg was administered intraperitoneally once in rats and the rats were sacrificed 5, 24, 48 and 72 hours later while in the control group, the salad oil of 5 mg/kg was administered intraperitoneally once. The mitochondria in brain tissue of rats were extracted to measure the membrane permeability and the activity of cytochrome C oxidase as well as the expression of cytochrome C in cortex and hippocampus.


RESULTS
After the treatment the permeability of mitochondrial membrane was significantly increased in the treated groups compared with the control group. The expression of cytochrome C was increased in cortex and hippocampus CA1 and CA2 5 h, 24 h and 48 h groups and CA4 24 h group (0.57 +/- 0.04, 0.67 +/- 0.09, 0.58 +/- 0.04) and (0.81 +/- 0.18) (P < 0.05 or P < 0.01) while there was no significant difference in the expression of cytochrome C in cortex and hippocampus CA2 72 h group and CA3 and CA4 5 h, 48 h and 72 h groups between the treated groups and the control group (P > 0.05). The activity of cytochrome C oxidase was inhibited (P < 0.01).


CONCLUSIONS
Deltamethrin can significantly increase the permeability of mitochondrial membrane and the expression of cytochrome C in brain tissue of rats.
Chen
Dan
D
Department of Health Toxicology, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
Shi
Nian
N
Huang
Xiao-wei
XW
Liu
Lin-lin
LL
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D045304:Cytochromes c / Q000096:biosynthesis*; D003576:Electron Transport Complex IV / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology*; D010539:Permeability / Q000187:drug effects; D011722:Pyrethrins / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D045304:Cytochromes c; D003576:Electron Transport Complex IV