Scale up of proteoliposome derived Cochleate production.
Cochleate are highly stable structures with promising immunological features. Cochleate structures are usually obtaining from commercial lipids. Proteoliposome derived Cochleate are derived from an outer membrane vesicles of Neisseria meningitidis B. Previously, we obtained Cochleates using dialysis procedures. In order to increase the production process, we used a crossflow system (CFS) that allows easy scale up to obtain large batches in an aseptic environment. The raw material and solutions used in the production process are already approved for human application. This work demonstrates that CFS is very efficient process to obtain Cochleate structures with a yield of more than 80% and the immunogenicity comparable to that obtained by dialysis membrane.
Zayas
Caridad
C
Immunology Department, Finlay Institute, P.O. Box 16017, Havana, Cuba. czayas@finlay.edu.cu
Bracho
Gustavo
G
Lastre
Miriam
M
González
Domingo
D
Gil
Danay
D
Acevedo
Reinaldo
R
del Campo
Judith
J
Taboada
Carlos
C
Solís
Rosa L
RL
Barberá
Ramón
R
Pérez
Oliver
O
D000276:Adjuvants, Immunologic / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D005260:Female; D051379:Mice; D038541:Neisseria meningitidis, Serogroup B / Q000737:chemistry; D011510:Proteolipids / Q000302:isolation & purification* / Q000494:pharmacology; D014462:Ultrafiltration
D000276:Adjuvants, Immunologic; D011510:Proteolipids; C020485:proteoliposomes