Neural stem-like cell line derived from a nonhematopoietic population of human umbilical cord blood.
The ability of stem and progenitor cells to proliferate and differentiate into other lineages is widely viewed as a characteristic of stem cells. Previously, we have reported that cells from a CD34(-) (nonhematopoietic) adherent subpopulation of human cord blood can acquire a feature of multipotential neural progenitors in vitro. In the present study, using these cord blood-derived stem cells, we have established a clonal cell line termed HUCB-NSCs (human umbilical cord blood-neural stem cells) that expresses several neural antigens and has been grown in culture for more than 60 passages. During this time, HUCB-NSCs retained their growth rate, the ability to differentiate into neuronal-, astrocyte-, and oligodendrocyte-like cells and displayed a stable karyotype. DNA microarray analysis of HUCB-NSCs revealed enhanced expression of selected genes encoding putative stem and progenitor cell markers when compared to other mononuclear cells. dBcAMP-induced HUCBNSCs were further differentiated into more advanced neuronal cells. This is the first report of the establishment and characterization of a nontransformed HUCB-NSC line that can be grown continuously in a monolayer culture and induced to terminal differentiation. These cells should further our understanding of the regulatory mechanisms involved in NSC self-renewal and differentiation.
Buzańska
Leonora
L
NeuroRepair Department, Medical Research Center, Warsaw, Poland.
Jurga
Marcin
M
Stachowiak
Ewa K
EK
Stachowiak
Michal K
MK
Domańska-Janik
Krystyna
K
D015415:Biomarkers; D002454:Cell Differentiation / Q000187:drug effects; D048708:Cell Growth Processes; D002460:Cell Line; D019070:Cell Lineage; D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000235:genetics; D003114:Colony-Forming Units Assay; D016895:Culture Media, Serum-Free; D000242:Cyclic AMP / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology* / Q000187:drug effects; D005796:Genes / Q000235:genetics; D006413:Hematopoietic System / Q000166:cytology; D006801:Humans; D007621:Karyotyping; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
D015415:Biomarkers; D016895:Culture Media, Serum-Free; D000242:Cyclic AMP