Salvianolic acid B modulates hemostasis properties of human umbilical vein endothelial cells.
Salviae miltiorrhizae (SM), a clinical, commonly used herb, can activate blood circulation and resolve stasis. We have investigated the effects of salvianolic acid B (Sal B), a pure compound extracted from the dried SM roots, on fibrinolytic (tissue-type plasminogen activator and plasminogen activator inhibitor, t-PA and PAI) and anticoagulant (thrombomodulin,TM) properties of cultured human umbilical vein endothelial cells (HUVECs). When HUVECs were treated with Sal B, a dose- (0.0125-0.5 mg/ml) and a time-dependent decrease in PAI activity were observed. PAI type 1 (PAI-1) antigen and PAI-1 mRNA expression significantly decreased compared to control values in the conditioned media of HUVECs pretreated with Sal B for 12 h. Moreover, TM activity reached a maximum stimulation of 1.25-fold over control levels in the pretreatment of Sal B for 12 h and t-PA and TM specific mRNA expression also increased (1.7- and 1.8-fold, respectively). In conclusion, Sal B increased the fibrinolytic and anticoagulant potential of cultured HUVECs by up-regulating the expression of t-PA and TM and by down-regulating the expression of PAI-1. These data suggest that Sal B is clinically effective because of its ability to change the gene expression profile of endothelial cells thereby preventing vascular events.
Shi
Chung-Sheng
CS
Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
Huang
Huey-Chun
HC
Wu
Hua-Lin
HL
Kuo
Cheng-Hsiang
CH
Chang
Bi-Ing
BI
Shiao
Ming-Shi
MS
Shi
Guey-Yueh
GY
D001572:Benzofurans / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology*; D005343:Fibrinolytic Agents / Q000378:metabolism; D006487:Hemostasis / Q000187:drug effects*; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism; D015849:Plasminogen Inactivators / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018180:Thrombomodulin / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D010959:Tissue Plasminogen Activator / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D053630:Depsides
D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D005343:Fibrinolytic Agents; D017395:Plasminogen Activator Inhibitor 1; D015849:Plasminogen Inactivators; D012333:RNA, Messenger; D018180:Thrombomodulin; D010959:Tissue Plasminogen Activator; C076944:salvianolic acid B; D053630:Depsides