Toxicity and toxin identification in Colomesus asellus, an Amazonian (Brazil) freshwater puffer fish.
Toxicity and toxin identification in Colomesus asellus, an Amazonian (Brazil) freshwater puffer fish. By using four different techniques--mouse bioassay, ELISA, HPLC and mass spectrometry-we evaluated the toxicity in the extracts of C. asellus, a freshwater puffer fish from the rivers of the Amazon, and identified for the first time the components responsible for its toxicity. The T20G10 monoclonal antibody raised against TTX, and employed in an indirect competitive enzyme immunoassay, showed very low affinity for the C. asellus extracts, indicating that TTX and its analogs are not the main toxic components of the extracts. This antibody was efficient in detecting presence of TTX in a total extract of Sphoeroides spengleri, which is one of the most toxic puffer fish found in the Atlantic coast. Extracts of C. asellus were toxic when administered intraperitonially into mice with an average toxicity of 38.6+/-12 mouse unit (MU)/g, while HPLC analysis indicated a lower toxin content (7.6+/-0 5MU/g). The HPLC profile showed no traces of TTX, but only the presence of PSPs (STX, GTX 2 and GTX 3). These toxins were also confirmed by electrospray ionization mass spectrometry.
Oliveira
Joacir Stolarz
JS
Department of Physiology, Biosciences Institute, University of São Paulo, Rua do Matão 101, Travessa 14, 05508 900 São Paulo, Brazil. jstolarz@usp.br
Fernandes
Solange Cristina Rego
SC
Schwartz
Carlos Alberto
CA
Bloch
Carlos
C
Melo
Jorge Alex Taquita
JA
Rodrigues Pires
Osmindo
O
de Freitas
José Carlos
JC
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D012530:Saxitoxin / Q000032:analysis* / Q000633:toxicity; D023721:Tetraodontiformes; D013779:Tetrodotoxin / Q000032:analysis* / Q000633:toxicity
D012530:Saxitoxin; D013779:Tetrodotoxin