Identification of bacteria in formalin-fixed, paraffin-embedded heart valve tissue via 16S rRNA gene nucleotide sequencing.
We applied 16S rRNA gene sequencing to identify bacterial species present in formalin-fixed, paraffin-embedded heart valve tissue. In 40% (12/30) of the cases, we were able to identify the bacterium to the species-genus level. For more recent cases (<or=4 years), the success rate was significantly improved, to 70% (P<0.001).
Imrit
Kavita
K
Department of Pathology, Clinical Microbiology Laboratory, Hackensack University Medical Center, 30 Prospect Ave., Hackensack, NJ 07601, USA.
Goldfischer
Michael
M
Wang
Jie
J
Green
Jaime
J
Levine
Jerome
J
Lombardo
Joseph
J
Hong
Tao
T
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005557:Formaldehyde; D020459:Genes, rRNA; D006351:Heart Valves / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D016707:Tissue Fixation
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D005557:Formaldehyde