title: Novel optical detection system for in vivo identification and localization of cervical intraepithelial neoplasia.
abstract: A noncontact optical detection system is developed for the in vivo identification and localization of high-grade cervical intraepithelial neoplasia (CIN 2,3). Diagnostic scans of the entire human cervix are performed following acetic acid application employing three integrated optical measurements: laser-induced fluorescence spectroscopy, white light diffuse reflectance spectroscopy, and video imaging. Full cervical scans comprising 499 interrogation locations at 1-mm spatial resolution are completed in 12 s. Diffuse reflectance and fluorescence spectra with signal-to-noise ratios of better than 100-to-1 are collected between 360 and 720 nm in increments of 1 nm, with an inherent spectral resolution of 8 nm. Glare reduction and optical vignetting are handled with a novel illumination scheme and subsequent spectral arbitration algorithms. The system is designed and found to be well below acceptable safe optical exposure levels. Typical reproducibility across multiple systems is approximately 5%, providing reliable and accurate detection of in vivo cervical neoplasia in normal clinical use.
authors.affiliation: MediSpectra, Incorporated, Lexington, Massachusetts 02421, USA. kschomacker@comcast.net
authors.forename: Kevin T
authors.initials: KT
authors.lastname: Schomacker
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Meese
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Jiang
authors.forename: Charles C
authors.initials: CC
authors.lastname: Abele
authors.forename: Karen
authors.initials: K
authors.lastname: Dickson
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Sum
authors.forename: Ross F
authors.initials: RF
authors.lastname: Flewelling
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods; D009361:Neoplasm Invasiveness; D055095:Optics and Photonics / Q000295:instrumentation; D010783:Photometry / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000145:classification / Q000175:diagnosis*; D002578:Uterine Cervical Dysplasia / Q000145:classification / Q000175:diagnosis*