Ionizing radiation suppresses FAP-1 mRNA level in A549 cells via p53 activation.
Ionizing radiation (IR) is known to upregulate cell surface Fas through p53 activation in various cells. However, the signaling pathway intermediating between p53 activation and cell surface Fas upregulation remains to be elucidated. Recently, Fas-associated phosphatase-1 (FAP-1) has been reported to associate with Fas and inhibit cell surface Fas expression. We evaluated the expression of FAP-1 mRNA following IR in A549 cells. Ionizing radiation inhibited the expression of FAP-1 mRNA. Pretreatment with p53 inhibitor pifithrin alpha cancelled the IR-induced downregulation of FAP-1 mRNA. These results suggest that IR-induced p53 activation may upregulate cell surface Fas via the down-modulation of FAP-1.
Yamada
Toru
T
The First Department of Internal Medicine, Faculty of Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Maruyama
Muneharu
M
Fujita
Tadashi
T
Miyabayashi
Koutarou
K
Shinoda
Chie
C
Kawagishi
Yukio
Y
Fujishita
Takashi
T
Hayashi
Ryuji
R
Miwa
Toshiro
T
Arai
Nobuki
N
Matsui
Shoko
S
Sugiyama
Eiji
E
Kobayashi
Masashi
M
D052160:Benzothiazoles; D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D054645:Protein Phosphatase 1; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011839:Radiation, Ionizing; D013844:Thiazoles / Q000494:pharmacology; D014050:Toluene / Q000031:analogs & derivatives / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*
D052160:Benzothiazoles; D012333:RNA, Messenger; D013844:Thiazoles; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor; D014050:Toluene; C121565:pifithrin; D054645:Protein Phosphatase 1; C516742:PTPN13 protein, human; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13; D017027:Protein Tyrosine Phosphatases