New RIA kit for the determination of progesterone in cows' milk.
A new, single-step, specific, simple, economical, and ready to use RIA test for the determination of progesterone in cow's milk was developed. Tubes coated with the specific polyclonal anti-progesterone antibody and progesterone-CMO-125I-histamine as the tracer were used. Progesterone in buffer and fat-free cow's milk, without progesterone were the matrix for the standard curve preparation. Fifty microL of milk and 500 microL of tracer were incubated in the tube for 2 h at room temperature, decanted, and coated. Assay range was 0-270 nmol of progesterone x 1(-1); sensitivity, <1 nmol x 1(-1); recovery, 94-104%; precision as CV%, 2.44-6%.
Byszewska-Szpocińska
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E
Radioisotope Centre POLATOM, Otwock-Swierk, Poland. e.byszewska@polatom.pl
Markiewicz
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A
D000818:Animals; D002417:Cattle; D005260:Female; D007457:Iodine Radioisotopes; D008892:Milk / Q000737:chemistry*; D011374:Progesterone / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007457:Iodine Radioisotopes; D011933:Reagent Kits, Diagnostic; D011374:Progesterone