title: Studies on the interaction between Oxaprozin-E and bovine serum albumin by spectroscopic methods.
abstract: The interaction between Oxaprozin-E and bovine serum albumin (BSA) was studied by spectroscopic methods including fluorescence and UV-vis absorption spectroscopy. The quenching mechanism of fluorescence of BSA by Oxaprozin-E was discussed to be a dynamic quenching procedure. The number of binding sites n and apparent binding constant K was measured by fluorescence quenching method. The thermodynamics parameter DeltaH, DeltaG, DeltaS were calculated. The results indicate the binding reaction was mainly entropy-driven and hydrophobic forces played major role in the binding reaction. The distance r between donor (BSA) and acceptor (Oxaprozin-E) was obtained according to Förster theory of non-radioactive energy transfer.
authors.affiliation: Department of Chemistry and Life Sciences, Xianning College, Xianning, PR China.
authors.forename: Shao-Fa
authors.initials: SF
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Han-Na
authors.initials: HN
authors.lastname: Hou
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Guang-Ya
authors.initials: GY
authors.lastname: Xiang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry; D002417:Cattle; D000077431:Oxaprozin; D011422:Propionates / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D011422:Propionates; D012710:Serum Albumin, Bovine; D000077431:Oxaprozin