title: Positional distribution of conjugated linoleic acid in triacylglycerol of Saccharomyces cerevisiae.
abstract: Saccharomyces cerevisiae was cultivated in the presence of cis-9,trans-11 or trans-10,cis-12 isomers of free conjugated linoleic acid (CLA), and the effects of the isomers on the regioisomerisms of triacylglycerol (TAG) of the yeast were elucidated. Both isomers constituted about 34% of all fatty acids and increased drastically the number of different TAG species. Nearly all of the species contained CLA in at least one sn-position. In the most abundant species analyzed (20% of total species), the cis-9,trans-11 isomer appeared in combination with monounsaturated fatty acids (C16:1, C:18:1) whereas trans-10,cis-12 isomer was most frequently present with a medium chain fatty acid (C10:0 or C12:0) in the sn-2 position and C16:0 in one of the end positions (14% of total species). With either isomer, the amount of TAG species in which CLA encompassed all sn-positions was ca. 4%. Thus, S. cerevisiae can be used to produce edible single cell oil characterized by very heterogeneous distribution of CLA among the different TAG species.
authors.affiliation: Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, P.O. Box 6100, FIN-02015 Hut, Finland. sanna.jaakola@hut.fi
authors.forename: Sanna
authors.initials: S
authors.lastname: Jaakola
authors.forename: Marjatta
authors.initials: M
authors.lastname: Vahvaselkä
authors.forename: Simo
authors.initials: S
authors.lastname: Laakso
mesh_terms: D005227:Fatty Acids / Q000032:analysis; D007536:Isomerism; D044243:Linoleic Acids, Conjugated / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D014280:Triglycerides / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated; D014280:Triglycerides