Fluorescent in situ hybridization and array comparative genomic hybridization: complementary techniques for genomic evaluation.
During the past few years a new high-throughput molecular technology, array comparative genomic hybridization, has received a great deal of attention. As a DNA-based tool, this technique is presumably more reproducible than expression arrays. In this review, I discuss how array comparative genomic hybridization is remarkably similar with regard to genome analysis to fluorescent in situ hybridization, a technique that is generally regarded as one of the more accurate and reproducible molecular techniques in diagnostic surgical pathology. A thorough understanding of this technology will be useful for all surgical pathologists in the near future, as this technology will no doubt have some influence on our daily practice.
Morrison
Carl
C
The Pathology Core Facility,Arthur James Cancer Hospital and Richard Solove Research Institute, The Ohio State University Medical School, Columbus 43210, USA. Carl.Morrison@osumc.edu
D020869:Gene Expression Profiling; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010340:Pathology, Surgical / Q000379:methods* / Q000639:trends