[Comparison of influences of different intervals of ultraviolet irradiation on calf thymus studied by Raman spectroscopy].
Raman spectroscopy was used to investigate the effect of different intervals of ultraviolet (UV) to calf thymus DNA in aqueous solution. At the same time, the different influences of these three UV parts were compared, and a complete damaging mechanism of UV to DNA was obtained. The results showed that UVC damages DNA in a complete way, while UVA and UVB damage DNA in a selective way, and the speed effect of UVB on DNA was faster than that of UVA. UVC seemed to be less valid in reducing the B conformation of DNA than UVA and UVB. Among all the groups of DNA, the pyrimidine bases and the deoxyribose experienced the most severe damages. Moreover, with UVA or UVB irradiation for 3 h, the photoreactivation of DNA occured. The experimental results partly supported the formation of several types of dimeric lesions between adjacent pyrimidine bases, most notably cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4PP) and the Dewar valence isomers.
Tang
Yu-long
YL
Institute of Laser Life Science, South China Normal University, Guangzhou, China.
Guo
Zhou-yi
ZY
D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors; D014466:Ultraviolet Rays
D004247:DNA; C427203:calf thymus DNA