Expression of SSX2 tumor antigen in baculovirus expression system and its application for screening of blood serum of melanoma patients.
OBJECTIVE
To clone, express in baculovirus expression system and purify SSX2 tumor antigen and to use it for screening of blood serum of melanoma patients.


METHODS
Cloning and expression of SSX2 antigen in Bac-to-Bac baculovirus expression system as His-tag fusion protein, expression of recombinant SSX2 in insect cells with following purification by affinity chromatography on Ni-NTA agarose, ELISA of blood serum of melanoma patients (n = 29) and healthy donors (n = 27) were used.


RESULTS
SSX2 was cloned in baculovirus expression system, expressed, purified using affinity chromatography and used in ELISA as antigen. Comparative analysis of blood serum of melanoma patients and healthy donors revealed higher level of SSX2-positivity of blood serum from the patients with cancer.


CONCLUSIONS
SSX2 antigen expressed in baculovirus expression system can be used for serological analysis of blood serum of oncological patients.
Kyyamova
R G
RG
Institute of Molecular Biology and Genetics, Kyiv, Ukraine. kyyamova@imbg.org.ua
Gryshkova
V S
VS
Zhyvoloup
A M
AM
D000818:Animals; D000951:Antigens, Neoplasm / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D016367:Baculoviridae / Q000235:genetics; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008545:Melanoma / Q000175:diagnosis*; D009363:Neoplasm Proteins / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D012097:Repressor Proteins / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D012878:Skin Neoplasms / Q000175:diagnosis*
D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; D012097:Repressor Proteins; C093962:synovial sarcoma X breakpoint proteins