title: An immunohistochemical study of the attachment mechanisms in different kinds of adhesive interfaces in teeth and alveolar bone of the rat.
abstract: OBJECTIVE
  This study was designed to examine the histological and immunohistochemical nature of different kinds of adhesive interfaces in the rat molar region under identical experimental conditions and to discuss the structural and functional similarities between these adhesive interfaces.
  
  
  METHODS
  Four kinds of adhesive interfaces - an initial attachment layer for principal fibers on the developing alveolar bone surface, a reattachment layer for principal fibers on resorbed alveolar bone surface, cement lines on the alveolar bone surface unrelated to the principal fibers, and the cemento-dentinal junction - were examined in 25-d-old male Wistar rats. Routine histological staining, immunohistochemical staining for bone sialoprotein and osteopontin, and digestion tests with trypsin were conducted.
  
  
  RESULTS
  The adhesive interfaces showed very similar histological and immunohistochemical features: they were intensely hematoxylin-stainable, deficient in collagen fibrils, and rich in bone sialoprotein and osteopontin. After trypsin treatment the four adhesive interfaces had lost immunoreactivity to bone sialoprotein and osteopontin, and the two adjacent tissue parts held together finally separated at the adhesive interfaces.
  
  
  CONCLUSIONS
  The above findings suggest that (i) the different types of adhesive interfaces in the rat molar region have a common structure in that they are filled with highly accumulated bone sialoprotein and osteopontin and deficient in collagen fibrils; (ii) accumulated bone sialoprotein and osteopontin are closely associated with the adhesion at the interfaces; and (iii) the adhesive interfaces have a similar developmental process.
authors.affiliation: Department of Oral Health Science, Hokkaido University, Graduate School of Dental Medicine, Sapporo, Japan. kapila@den.hokudai.ac.jp
authors.forename: A K S
authors.initials: AK
authors.lastname: Arambawatta
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Wakita
mesh_terms: D000268:Adhesiveness; D016301:Alveolar Bone Loss / Q000503:physiopathology*; D000539:Alveolar Process / Q000502:physiology*; D000818:Animals; D003739:Dental Cementum / Q000502:physiology*; D024022:Fibrillar Collagens / Q000032:analysis / Q000502:physiology; D007124:Immunoenzyme Techniques; D058574:Integrin-Binding Sialoprotein; D008297:Male; D008963:Molar; D053495:Osteopontin; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000032:analysis / Q000502:physiology; D013499:Surface Properties
chemical_list: D024022:Fibrillar Collagens; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin