Is the sigma2 receptor a histone binding protein?
Starting from the high affinity sigma(2) receptor ligand 2, (PB28), we synthesized amino derivative 4 and coupled it to an NHS-ester activated sepharose stationary phase column to elute a crude protein prepared by lysed human SK-N-SH neuroblastoma cells. We characterized the SDS-PAGE gel electrophoresis stained bands by MALDI-MS and LC-MS-MS analysis. The MASCOT MS-MS ion search program led to the identification of the protein components. The six eluted proteins had a molecular weight ranging from 13 kDa to 26 kDa and were human histone proteins. A human 40S ribosomal protein S3 (SwissProt accession number: P23396) was also identified as a comigrated band. The human histone proteins that were characterized were H3.3A histone (NCBI accession number: 51859376), H2B histone (NCBI accession number: 1568557), H2A.5 histone (NCBI accession number: 70686), H1 (NCBI accession number: 22770677), and H2.1 histone (SwissProt accession number: P16403). These results disclosed a dual hypothesis about the sigma(2) receptor, that is, that it is formed by histones or that the sigma(2) ligands also bind histone proteins.
Colabufo
Nicola Antonio
NA
Dipartimento Farmacochimico, Università degli Studi di Bari, via Orabona 4, 70125 Bari, Italy. colabufo@farmchim.uniba.it
Berardi
Francesco
F
Abate
Carmen
C
Contino
Marialessandra
M
Niso
Mauro
M
Perrone
Roberto
R
D015057:1-Naphthylamine / Q000031:analogs & derivatives / Q000737:chemistry; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D008024:Ligands; D013058:Mass Spectrometry; D009447:Neuroblastoma; D017480:Receptors, sigma / Q000737:chemistry / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000302:isolation & purification / Q000378:metabolism
C513155:8-(3-(4-cyclohexylpiperazin-1-yl)propyl)-4-methoxy-5,6,7,8-tetrahydronaphthalen-1-ylamine; D006657:Histones; D007202:Indicators and Reagents; D008024:Ligands; D017480:Receptors, sigma; D012269:Ribosomal Proteins; C106107:sigma-2 receptor; D015057:1-Naphthylamine