title: ChIP-chip comes of age for genome-wide functional analysis.
abstract: In the post-genome era, attention has focused on the functions of genome sequences and how they are regulated. The emerging epigenomic changes and the interactions between cis-acting elements and protein factors may play a central role in gene regulation. To understand the crosstalk between DNA and protein on a genome-wide scale, one emerging technique, called ChIP-chip, takes the strategy of combining chromatin immunoprecipitation with microarray. This new high-throughput strategy helps screen the targets of critical transcription factors and profile the genome-wide distribution of histone modifications, which will enable the feasibility of conducting a large-scale study, such as the Human Epigenome Project.
authors.affiliation: Departments of Molecular Genetics and Molecular Virology, Immunology, and Medical Genetics, Division of Human Cancer Genetics, Comprehensive Cancer Center, Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Jiejun
authors.initials: J
authors.lastname: Wu
authors.forename: Laura T
authors.initials: LT
authors.lastname: Smith
authors.forename: Christoph
authors.initials: C
authors.lastname: Plass
authors.forename: Tim H-M
authors.initials: TH
authors.lastname: Huang
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D023281:Genomics / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*