title: Cytotoxic diterpenoids from Podocarpus madagascariensis from the Madagascar rainforest.
abstract: Bioassay-directed fractionation of an extract of the root and bark of Podocarpus madagascariensis resulted in the isolation of a new totarol diterpenoid (1) in addition to the three known cytotoxic diterpenoids 19-hydroxytotarol (2), totaradiol (3), and 4beta-carboxy-19-nor-totarol (4). The structure of the new compound 1 was established as methyl-13-hydroxy-14-isopropyl-9(11),12,14(8)-podocarpatriene-19-oate on the basis of 1D and 2D NMR spectroscopic interpretation and methylation of 4. All the compounds exhibited cytotoxic activity against the A2780 ovarian cancer cell line.
authors.affiliation: Department of Chemistry, M/C 0212, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0212, USA.
authors.forename: Micah
authors.initials: M
authors.lastname: Reynolds
authors.forename: V S Prakash
authors.initials: VS
authors.lastname: Chaturvedula
authors.forename: Fidisoa
authors.initials: F
authors.lastname: Ratovoson
authors.forename: Rabodo
authors.initials: R
authors.lastname: Andriantsiferana
authors.forename: Vincent E
authors.initials: VE
authors.lastname: Rasamison
authors.forename: Rebecca C
authors.initials: RC
authors.lastname: Guza
authors.forename: David G I
authors.initials: DG
authors.lastname: Kingston
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D032462:Cycadopsida / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017753:Ecosystem; D005260:Female; D006801:Humans; D008270:Madagascar; D019906:Nuclear Magnetic Resonance, Biomolecular; D010051:Ovarian Neoplasms / Q000188:drug therapy; D024301:Plant Bark / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes