title: Rapid multiplexing and simultaneous detection of human spermatogenetic failure with a 12 lane microchip electrophoresis system.
abstract: For the amplification and ultrafast separation of the genetic markers and DNA sequences that are related to human male infertility, a multiplex PCR for amplifying three DNA sequence-tagged sites (STS) located on the human Y chromosome with possible roles in the spermatogenesis process has been designed and applied followed by separation on a microchip. First, the optimum T(m) degree for the three DNA markers was optimized and determined experimentally, and the three DNA STS were amplified. These three DNA markers were then separated on a 12-lane microchip electrophoresis system, which can analyze the DNA markers on 12 channels simultaneously. The combination of these two technologies, multiplex PCR and microchip electrophoresis, allows the analysis of 36 DNA markers (12x3) within only 180 s.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Japan. jabasini1@yahoo.com
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jabasini
authors.forename: Ashraf Abdulazim
authors.initials: AA
authors.lastname: Ewis
authors.forename: Maged
authors.initials: M
authors.lastname: Fouad
authors.forename: Fuquan
authors.initials: F
authors.lastname: Dang
authors.forename: Guichen
authors.initials: G
authors.lastname: Ping
authors.forename: Toshikatsu
authors.initials: T
authors.lastname: Shinka
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakahori
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D004586:Electrophoresis / Q000379:methods; D005819:Genetic Markers; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis* / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA