Suppression of tumor growth by intra-muscular transfer of naked DNA encoding adrenomedullin antagonist.
We have recently reported that the intra-tumoral injection of adrenomedullin (AM) antagonist (AMA; AM (22-52)) peptides significantly reduced the in vivo growth of a pancreatic cancer cell line in severely combined immunodeficient (SCID) mice. In the present study, we examined the effects of intra-tumoral and intra-muscular transfers of naked DNA encoding AMA on the in vivo growth of cancer cell lines. We demonstrate that these treatments induce the regression of a pancreatic cancer cell line and a breast cancer cell line inoculated in SCID mice. Furthermore, CD31-positive cells disappear completely from tumor tissues, following treatment, indicating that neo-vascularization is entirely inhibited. These results suggest that the intra-tumoral or intra-muscular transfer of naked DNA encoding AMA might be a promising alternative modality for treating human cancers.
Miseki
T
T
Division of Cancer Biology, Institute for Genetic Medicine, Hokkaido University, Hokkaido, Japan.
Kawakami
H
H
Natsuizaka
M
M
Darmanin
S
S
Cui
H Y
HY
Chen
J
J
Fu
Q
Q
Okada
F
F
Shindo
M
M
Higashino
F
F
Asaka
M
M
Hamuro
J
J
Kobayashi
M
M
D053607:Adrenomedullin / Q000037:antagonists & inhibitors* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage*; D017931:DNA Primers; D006801:Humans; D015552:Injections, Intralesional; D051379:Mice; D016513:Mice, SCID; D009132:Muscles; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics
D017931:DNA Primers; D012333:RNA, Messenger; D053607:Adrenomedullin; D004247:DNA