Self-assembled monolayers of thiol-end-labeled DNA at mercury electrodes.
We show for the first time that thiol-end-labeled oligodeoxynucleotides form self-assembled monolayer (SAM) at Hg electrodes. Changes in voltammetric signals due to cytosine and Hg-S bond reduction indicated changes in positioning of the HS-(TTC)(7) molecules at the electrode with increasing HS-(TTC)7 concentration from lying flat with respect to SAMs, forming upright-standing molecules. This SAM behaved as an insulator not allowing electron transfer between [Ru(NH3)6]3+ and the electrode. Different adsorption modes of thiolated and thiol-free DNAs were observed.
Ostatna
Veronika
V
Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno, Czech Republic.
Palecek
Emil
E
D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D008567:Membranes, Artificial; D008628:Mercury / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
D008567:Membranes, Artificial; D013438:Sulfhydryl Compounds; D004247:DNA; D008628:Mercury