Protective effect of carvedilol on oxidative stress induced by okadaic acid in N1E-115 cells.
The effect of carvedilol on oxidative and cell damage induced by okadaic acid in N1E-115 cells were studied. The effects of okadaic acid were evaluated as changes in: the quantity of lipid peroxidation products, protein carbonyl groups, reduced glutathione content (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase and total lactate dehydrogenase (cell LDH). Additionally, a dose of carvedilol (10(-5)M) was added 2h before incubation with okadaic acid (50 nM) and was present until the end of the experiment (2h later added okadaic acid). Our results reveal that okadaic acid induces oxidative stress and an increase of cell LDH in N1E-115 cells, whereas carvedilol prevented the changes prompted by okadaic acid. In conclusion, the data show the protective effect of carvedilol, as well as its ability to modify cell response to okadaic acid, involving like cytoprotective mechanism its antioxidative properties.
Túnez
Isaac
I
Department of Biochemistry and Molecular Biology, School of Medicine, University of Cordoba, Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain. fm2tufii@uco.es
Collado
Juan A
JA
Medina
Francisco J
FJ
Muñoz
M Carmen
MC
Gordillo
Rafael
R
Sampedro
Concepción
C
Moyano
María J
MJ
Feijóo
Montserrat
M
Muntané
Jordi
J
Montilla
Pedro
P
D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D000077261:Carvedilol; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D004347:Drug Interactions; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008550:Melatonin / Q000378:metabolism; D051379:Mice; D009447:Neuroblastoma; D019319:Okadaic Acid / Q000633:toxicity*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D011412:Propanolamines / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D002227:Carbazoles; D011412:Propanolamines; D011506:Proteins; D000077261:Carvedilol; D019319:Okadaic Acid; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D008550:Melatonin