title: Very small size proteoliposomes derived from Neisseria meningitidis: an effective adjuvant for dendritic cell activation.
abstract: Recent findings in the interactions between pathogens and the innate immune system, particularly with dendritic cells (DC), have opened new opportunities for adjuvants designs. We have elaborated a new approach, in which gangliosides are incorporated into the outer membrane complex of Neisseria meningitidis to form very small size proteoliposomes (VSSP). VSSP demonstrated a unique ability to render highly tolerated gangliosides immunogenic. These results drove our attention to the immunopotentiating properties of VSSP. Here we examined the VSSP adjuvant effect on dendritic cell activation. Also the role of LPS on this effect was dissected. This study reveals that VSSP is a potent adjuvant for DC activation.
authors.affiliation: Vaccine's Department, Centre of Molecular Immunology, 216 esq 15, Atabey, Playa, CP 16040, C. Habana, Cuba. circe@ict.cim.sld.cu
authors.forename: Circe
authors.initials: C
authors.lastname: Mesa
authors.forename: Joel
authors.initials: J
authors.lastname: de León
authors.forename: Kevin
authors.initials: K
authors.lastname: Rigley
authors.forename: Luis E
authors.initials: LE
authors.lastname: Fernández
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D005732:Gangliosides / Q000378:metabolism; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009345:Neisseria meningitidis / Q000737:chemistry; D010316:Particle Size; D011510:Proteolipids / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D005732:Gangliosides; D011510:Proteolipids; C020485:proteoliposomes