title: Applicability of the modified universal calibration of gel permeation chromatography on proteins.
abstract: The modified universal calibration of gel permeation chromatography (GPC) has been applied in the case of native proteins. Plotting log([eta]M/Phi) versus elution volume, instead of log[eta]M versus elution volume used till now, we obtain unique curves with different proteins and non-proteonic polymers ([eta]: intrinsic viscosity, M: molecular mass, Phi: Flory's parameter). The values of Flory's parameter Phi are calculated for each protein using an indirect method based on GPC.
authors.affiliation: Department of Chemical Engineering, University of Patras, 26504 Patras, Greece. dondos@chemeng.upatras.gr
authors.forename: Anastasios
authors.initials: A
authors.lastname: Dondos
mesh_terms: D002138:Calibration; D002850:Chromatography, Gel / Q000379:methods*; D008433:Mathematics; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D014783:Viscosity
chemical_list: D011506:Proteins