[Effect of eosin Y on ATPase activity of actomyosine complex of the uterus smooth muscle].
The effect of eosin Y (2,4,5,7 - tetrabromofluorescein; 0.1-100 microM) on ATPase activity smooth muscle actomyosine was studied. The inhibition coefficient i50 of ATPase activity with eosin Y was 0.74 +/- 0.07 microM. The inhibitor decreased V(max) of actomyosine ATPase for ATP, but no influence on affinity of actomyosine for ATP was observed. It is suggested that eosin Y inhibits actomyosine ATPase activity noncompetitively in respect of ATP.
Labyntseva
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Kosterin
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D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004801:Eosine Yellowish-(YS) / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D009130:Muscle, Smooth / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009218:Myosins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013552:Swine; D014599:Uterus / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
D004791:Enzyme Inhibitors; D000255:Adenosine Triphosphate; D009218:Myosins; D002118:Calcium; D004801:Eosine Yellowish-(YS)