title: Initial responses of human osteoblasts to sol-gel modified titanium with hydroxyapatite and titania composition.
abstract: Sol-gel thin films of hydroxyapatite (HA) and titania (TiO(2)) have received a great deal of attention in the area of bioactive surface modification of titanium (Ti) implants. Sol-gel coatings were developed on Ti substrates of pure HA and TiO(2) and two composite forms, HA+10% TiO(2) and HA+20% TiO(2), and the biological properties of the coatings were evaluated. All the coating layers exhibited thin and homogeneous structures and phase-pure compositions (either HA or TiO(2)). Primary human osteoblast cells showed good attachment, spreading and proliferation on all the sol-gel coated surfaces, with enhanced cell numbers on all the coated surfaces relative to uncoated Ti control at day 1, as observed by MTT assay and scanning electron microscopy. Cell attachment rates were also enhanced on the pure HA coating relative to control Ti. The pure HA and HA+10% TiO(2) composite coating furthermore enhanced proliferation of osteoblasts at 4 days. Moreover, the gene expression level of several osteogenic markers including bone sialoprotein and osteopontin, as measured by RT-PCR at 24h, was shown to vary according to coating composition. These findings suggest that human primary bone cells show marked and rapid early functional changes in response to HA and TiO(2) sol-gel coatings on Ti.
authors.affiliation: Division of Biomaterials and Tissue Engineering, Eastman Dental Institute, University College London, 256 Gray's Inn Road, London WC1X 8LD, UK.
authors.forename: Jamie
authors.initials: J
authors.lastname: Harle
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
authors.forename: Nicky
authors.initials: N
authors.lastname: Mordan
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Knowles
authors.forename: Vehid
authors.initials: V
authors.lastname: Salih
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology; D005782:Gels; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000235:genetics; D014025:Titanium / Q000494:pharmacology*
chemical_list: D020099:Coated Materials, Biocompatible; D005782:Gels; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; C009495:titanium dioxide; D017886:Durapatite; D014025:Titanium