Micellar electrokinetic capillary chromatography determination of zinc bacitracin and nystatin in animal feed.
An MEKC procedure was developed for the separation of zinc bacitracin (Zn-BC) and nystatin (NYS) in mixtures and in animal feedstuff. The running buffer was 15 mM borate/19 mM phosphate, pH 8.2, containing 20 mM SDS and 10% v/v methanol. Samples were run at 25 degrees C, the applied voltage was 25 kV, and an additional pressure of 5 mbar was applied. Both analytes were detected by UV simultaneously at 215 nm, Zn-BC alone at 192 and 254 nm, and NYS alone at 305 nm. The method was shown to be specific, accurate (recoveries were 100.0 +/- 0.6% and 100.1 +/- 0.6% for Zn-BC and NYS, respectively), linear over the tested range (correlation coefficients 0.9991 and 0.9994), and precise (RSD below 1.3% for both analytes). The method was applied to determine Zn-BC and NYS as additives in animal feed.
Injac
Rade
R
Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Ljubljana, Ljubljana, Slovenia.
Kac
Javor
J
Mlinaric
Ales
A
Karljikovic-Rajic
Katarina
K
D000821:Animal Feed; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001414:Bacitracin / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D009761:Nystatin / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D009761:Nystatin; D001414:Bacitracin