Selective identification of Valpha14i T cells using slide-immobilized, CD1d-antigen complexes.
The ability to correlate changes in antigen-reactive lymphocytes with disease will provide information needed to develop strategies for combating illness. One critical group of lymphocytes are the CD1-restricted T cells. It is desirable to use CD1 molecules in an array format to query CD1-restricted lymphocytes in humans. To investigate the feasibility of this technique, we employed mCD1d and alpha-galactosylceramide to demonstrate that-slide immobilized, CD1d-alpha-GalCer complexes capture an NKT cell hybridoma in the presence of a competitor. The success of this scheme represents the first step toward the development of CD1-antigen arrays that could be used to profile biological samples.
Zullo
Alfred J
AJ
Department of Biological Sciences and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907-2054, USA.
Brutkiewicz
Randy R
RR
Taparowsky
Elizabeth J
EJ
D000818:Animals; D018949:Antigens, CD1 / Q000276:immunology*; D055610:Antigens, CD1d; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D018949:Antigens, CD1; D055610:Antigens, CD1d; C526877:CD1D protein, human