Sarcolemma-specific autoantibodies in canine inflammatory myopathy.
Inflammatory myopathies (IM) are relatively common in dogs with an increased incidence in the Boxer and Newfoundland breeds. Here, we show that a high proportion of affected Boxers and Newfoundlands have circulating autoantibodies against unknown sarcolemma antigens, that are muscle-specific but not species specific. We further show that the autoantigen can be extracted from muscle membranes with non-ionic detergent, and that such detergent extracts can be used in a sensitive ELISA for detection and quantitation of antibodies. The relatively high incidence of IM with autoantibodies in selected breeds of dogs indicates a genetic predisposition for a particular form of IM. In these breeds, this form of IM could be diagnosed and monitored with a simple serum assay.
Hankel
Sabine
S
The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
Shelton
G Diane
GD
Engvall
Eva
E
D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D001707:Biopsy, Needle; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D020022:Genetic Predisposition to Disease; D008297:Male; D018482:Muscle, Skeletal / Q000276:immunology / Q000473:pathology; D009220:Myositis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D012508:Sarcolemma / Q000276:immunology* / Q000473:pathology
D001323:Autoantibodies