Persistence of a plasma melatonin rhythm in constant darkness and its inhibition by constant light in the sleepy lizard, Tiliqua rugosa.
This study determined whether a blood plasma melatonin rhythm persists in constant photothermal environments in the sleepy lizard, Tiliqua rugosa. It builds upon an earlier investigation which provided equivocal results as to whether an in vivo melatonin rhythm persists in constant dark (DD) and light (LL) and temperature in this species. Using more frequent sampling points and new assay techniques, the present study showed that the melatonin rhythm persisted for at least 6 days at temperatures of 25 and 33 degrees C in constant dark (DD). The melatonin rhythm, however, was largely eliminated in constant light (LL) at 33 degrees C, thereby contradicting some previous findings in other species of reptiles where melatonin levels were apparently insensitive to an unexpected pulse of light at night. These results demonstrate that the sleepy lizard has a persistent, possibly circadian rhythm of melatonin in DD and constant temperature, and that the rhythm is inhibited by LL and constant temperature. Therefore, the sleepy lizard pineal gland may be an independent oscillator capable of driving the melatonin rhythm and be a transducer of the seasonally changing external photothermal environment.
Firth
Bruce T
BT
Department of Anatomical Sciences, University of Adelaide, Adelaide, Australia. bruce.firth@adelaide.edu.au
Belan
Ingrid
I
Kennaway
David J
DJ
D000818:Animals; D003624:Darkness; D005260:Female; D008027:Light; D008116:Lizards / Q000502:physiology*; D008297:Male; D008550:Melatonin / Q000097:blood*; D011863:Radioimmunoassay
D008550:Melatonin