DNA damage response and mutagenesis in mouse embryonic stem cells.
Mutation in embryonic stem (ES) cells can potentially compromise multiple cell lineages and affect the well-being of subsequent generations. Thus, ES cells require sensitive mechanisms to maintain genomic integrity. One mechanism involves suppression of mutation. A complementary mechanism is to regulate the cell cycle checkpoint and facilitate cell death. Here, we describe the detailed protocols we have used to investigate DNA damage response and mutagenesis in mouse ES cells.
Hong
Yiling
Y
Department of Cell Biology, Neurobiology and Anatomy, University of Cincinnati College of Medicine, OH, USA.
Cervantes
Rachel B
RB
Stambrook
Peter J
PJ
D000228:Adenine Phosphoribosyltransferase / Q000172:deficiency / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D004247:DNA / Q000235:genetics; D004249:DNA Damage; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D018390:Gene Targeting; D005822:Genetic Vectors; D042822:Genomic Instability; D051379:Mice; D008809:Mice, Inbred C3H; D018345:Mice, Knockout; D016296:Mutagenesis; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy
D004247:DNA; D000228:Adenine Phosphoribosyltransferase