title: In-vivo assessment of antidiabetic and antioxidant activities of grapevine leaves (Vitis vinifera) in diabetic rats.
abstract: The acute and the subacute (15 days) hypoglycaemic and antihyperglycaemic effect of the two different doses (250, 500 mg/kg) of the aqueous extract from the leaves of Vitis vinifera L. were evaluated in this study. The aqueous extract was further fractionated through successive solvent extractions and the acute effect of different doses of its subfractions, 25 mg/kg for ethylacetate fraction, 80 mg/kg for n-butanol fraction and 375 mg/kg for remaining aqueous fraction were investigated using normal, glucose-hyperglycaemic and streptozotocin-induced diabetic rats. Blood glucose levels were measured according to the glucose oxidase method. Tolbutamide was used as a reference drug at a dose of 100 mg/kg. The antioxidant activity of the test samples was studied in the liver, kidney and heart tissues of diabetic rats by measuring malondialdehyde (MDA) and glutathion (GSH) levels. All results were compared to the diabetic control groups. The results showed that EtOAc Fr. was rich in polyphenolics and possessed a significant antihyperglycaemic and antioxidant activity equipotent with the reference hypoglycaemic agent (tolbutamide), when evaluated in diabetic rats.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Hipodrom 6330, Ankara, Turkey. nsendogdu@gzi.edu.tr
authors.forename: Nilüfer
authors.initials: N
authors.lastname: Orhan
authors.forename: Mustafa
authors.initials: M
authors.lastname: Aslan
authors.forename: Didem Deliorman
authors.initials: DD
authors.lastname: Orhan
authors.forename: Fatma
authors.initials: F
authors.lastname: Ergun
authors.forename: Erdem
authors.initials: E
authors.lastname: Yeşilada
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000627:therapeutic use*; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008519:Medicine, Traditional; D009206:Myocardium / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D013997:Time Factors; D014421:Turkey; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D008315:Malondialdehyde; D013311:Streptozocin; D005978:Glutathione