title: Thermostability of heterophile antibodies from human sera infected with Schistosoma mansoni and geo-helminths. An immuno-metric statistical analysis.
abstract: Antibody in human sera that induces lysis of sheep erythrocytes in hemolytic assay was investigated. The present study showed that the presence in serum of the thermostable cytolytic anti-sheep red blood cells antibodies is dependent on the Schistosoma mansoni infection, and this is more frequent in adults than in children. The thermostable characteristic of hemolysins in normal sera was not dependent on the presence of Ascaris lumbricoides, Trichuris trichiura or hookworm geo-helminths. Further, thermostable complement-activating heterophile antibodies were noticed in children in association with massive number of S. mansoni eggs. The results were obtained by using the z- and the chi-square tests. The z-test allows us to formulate a one-sided alternative, i.e., a tendency of one of the attributes. On the other hand, the chi-square test analyzes the independence between attributes by using a contingency table. Besides the obtained results being interesting in the field of schistosomiasis mansoni, they can provide a new insight into the use of statistics in medical science.
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, 30270-901 Belo Horizonte, MG, Brazil.
authors.forename: Munir
authors.initials: M
authors.lastname: Chamone
authors.forename: Gregorio S
authors.initials: GS
authors.lastname: Atuncar
authors.forename: Paulo Marcos Zech
authors.initials: PM
authors.lastname: Coelho
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000910:Antibodies, Heterophile / Q000097:blood / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D002648:Child; D004912:Erythrocytes / Q000276:immunology*; D005243:Feces / Q000469:parasitology; D006376:Helminths / Q000276:immunology; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D007118:Immunoassay / Q000379:methods; D015233:Models, Statistical; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000276:immunology* / Q000378:metabolism; D012555:Schistosomiasis mansoni / Q000276:immunology*; D012756:Sheep; D013696:Temperature
chemical_list: D000910:Antibodies, Heterophile; D000913:Antibodies, Protozoan