Efficient intracellular multiplication of Legionella pneumophila in human monocytes requires functional host cell L-type calcium channels.
The infectious agent of Legionnaires' disease, Legionella pneumophila, multiplies intracellularly in a variety of eukaryotic cells. Genistein, a tyrosine kinase inhibitor, has been shown to block intracellular replication of L. pneumophila without harming the infected host cell. The present study has been performed to investigate the underlying mechanism. We demonstrate that inhibition of intracellular bacterial growth by genistein is not mediated by its protein tyrosine kinase-modulating effect but by inhibition of L-type calcium channels of the infected host cell.
Wieland
Hagen
H
Department of Livestock Biotechnology, WZW TU Munich, Freising, Germany. hagen.wieland@wzw.tum.de
Hechtel
Nicole
N
Faigle
Marion
M
Neumeister
Birgid
B
D002118:Calcium / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D020847:Estrogen Receptor Modulators / Q000494:pharmacology; D019833:Genistein / Q000378:metabolism; D006801:Humans; D016952:Legionella pneumophila / Q000254:growth & development*; D007877:Legionnaires' Disease / Q000382:microbiology*; D009000:Monocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism
D020746:Calcium Channels, L-Type; D020847:Estrogen Receptor Modulators; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D002118:Calcium