[Inheritance of the foreign gene 1Ax1 in transgenic wheat (Triticum aestivum L.) with gene cassettes lacking vector backbone sequences].
Transformation with gene minimal expression cassettes without vector backbone sequence is a new transformation technique. In the current research, the inheritance of the foreign 1Ax1 gene cassettes were analyzed in transgenic plants recovered with gene cassettes lacking vector backbone sequences. The results showed that linear 1Ax1 gene cassettes were stably expressed and separated at the rate of 3 to 1 in the seeds of T1 generation. It suggested the functional copies of 1Ax1 gene cassettes were integrated in one sites, abiding by Mendelian genetic law. It revealed firstly the feasibility of the transformation technique with gene cassettes, and proved the inheritance of transgene cassettes in subsequent generations.
Yao
Qin
Q
China-UK HUST-RRes Genetic Engineering and Genomics Joint Lab, Huazhong University of Science and Technology, Wuhan 430074, China. q.amandayao@gmail.com
Cong
Ling
L
Wang
Yue-Sheng
YS
Chen
Ming-Jie
MJ
Yang
Guang-Xiao
GX
He
Guang-Yuan
GY
D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
D010940:Plant Proteins