Identification of the first neuropeptides from the CNS of Hemiptera: CAPA peptides of the southern green stinkbug Nezara viridula (L.).
A direct mass spectrometric investigation of nerve homologs of the abdominal perisympathetic organs was employed to reveal the first and complete sequences of CAPA peptides from a hemipteran species, the southern green stinkbug Nezara viridula. Side-chain fragmentations allowed the assignment of internal Leu/Ile; on-plate acetylation was used to distinguish between the mass-related Lys and Gln. The following sequences were obtained: DQLFPFPRV-NH(2) (CAPA-PVK-1), EQLIPFPRV-NH(2) (CAPA-PVK-2), and NGSAGNGGLWFGPRL/I-NH(2) (CAPA-PK). CAPA-PVKs are associated with the regulation of diuresis in insects, and identification of those native to a hemipteran will provide the experimental basis to better understand regulation of water balance in this family of insects.
Predel
Reinhard
R
Areawide Pest Management Research, Southern Plains Agricultural Research Center, USDA, 2881 F/B Road, College Station, TX 77845, USA. b6prre@pan.zoo.uni-jena.de
Russell
William K
WK
Neupert
Susanne
S
Russell
David H
DH
Esquivel
Jesus F
JF
Nachman
Ronald J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000737:chemistry*; D006430:Hemiptera / Q000235:genetics*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009479:Neuropeptides