Study of biological safety of scaffold material with heterologous deproteinized bone.
OBJECTIVE
To evaluate the biological safety of manufactured heterologous deproteinized bone and to provide an experimental basis for clinical applications.


METHODS
Deproteinized bone (10 mm) and leaching liquor were made from pig ribs with a series of physical and chemical methods, then were evaluated through acute and subacute toxicity test, hemolysis test, pyrogen test, intracutaneous test, intramuscular implantation test and cytotoxity test.


RESULTS
No obvious toxicity, hemolysis, pyrogenic characteristics, skin irritation, inflammatory reaction after intramuscular implantation and cytotoxity were observed.


CONCLUSIONS
The heterologous deproteinized bone has good biological safety and meets all the demands of scaffold material for tissue engineering.
Liu
Lei
L
Center for Joint Surgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
Tang
Kang-lai
KL
Yang
Liu
L
Jing
Yue-kui
YK
Li
Qi-hong
QH
D000818:Animals; D016025:Bone Transplantation; D003601:Cytotoxicity Tests, Immunologic; D006461:Hemolysis; D051379:Mice; D011817:Rabbits; D023822:Tissue Engineering; D014183:Transplantation, Heterologous