title: Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: distribution of G/P types and finding of a rare G12 strain.
abstract: Between May and August in 2003, a total of 251 fecal samples were collected from children and adults with diarrhea (5 inpatients and 246 outpatients) at a private hospital in the city of Ponta Grossa, the state of Paraná, Brazil. Group A rotavirus was detected in 71 of 251 (28.3%) specimens: 55 (77.5%) from children under 5 years of age and 16 (22.5%) from individuals aged 6-72 years. All 71 strains exhibited a "long" RNA pattern when analyzed by PAGE. Sixty-one positive samples that yielded enough RNA were submitted to PCR genotyping. The most frequent G/P genotype combination detected was G1P[8] (86.9%; 53/61) followed by G9P[8] (3.3%; 2/61) and G12P[9] (1.6%; 1/61). Rotaviruses with G2, G3, G4, P[4], or P[6] specificity were not detected. For three strains (4.9%) bearing G1 genotype, the VP4 specificity could no be determined, and two specimens (3.3%) remained G/P non-typeable. One rotavirus strain (HC91) bearing G12P[9] genotype with a "long" electropherotype was isolated from an 11-month-old boy with diarrhea for the first time in Brazil. The cell-culture grown HC91 strain was shown to belong to serotype G12 by neutralization.
authors.affiliation: Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Pietruchinski
authors.forename: Fabrício
authors.initials: F
authors.lastname: Benati
authors.forename: Flávio
authors.initials: F
authors.lastname: Lauretti
authors.forename: Jonas
authors.initials: J
authors.lastname: Kisielius
authors.forename: Marli
authors.initials: M
authors.lastname: Ueda
authors.forename: Eduardo M
authors.initials: EM
authors.lastname: Volotão
authors.forename: Caroline C
authors.initials: CC
authors.lastname: Soares
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Rosa Elisa C
authors.initials: RE
authors.lastname: Linhares
authors.forename: Carlos
authors.initials: C
authors.lastname: Nozawa
authors.forename: Norma
authors.initials: N
authors.lastname: Santos
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D003625:Data Collection; D003967:Diarrhea / Q000453:epidemiology*; D005243:Feces / Q000821:virology; D016304:Hospitals, Private; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D017720:Molecular Epidemiology; D009500:Neutralization Tests; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology*; D013045:Species Specificity; D014505:Urban Population
chemical_list: D012367:RNA, Viral