title: Generation and characterization of monkey embryonic stem cells.
abstract: Nonhuman primate embryonic stem (ES) cells are very important for preclinical research of the medical application of human ES cells. Because primate ES cells show significant differences from mouse ES cells, we have been optimizing protocols for the establishment and maintenance of monkey ES cells. The latest methods for derivation and culture of cynomolgus monkey ES cells are described in detail.
authors.affiliation: Laboratory of Embryonic Stem Cell Research, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Suemori
authors.forename: Norio
authors.initials: N
authors.lastname: Nakatsuji
mesh_terms: D000818:Animals; D015415:Biomarkers; D001755:Blastocyst / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D006801:Humans; D007621:Karyotyping; D008252:Macaca fascicularis / Q000196:embryology*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology*; D011247:Pregnancy
chemical_list: D015415:Biomarkers