title: Investigation of living pancreas tumor cells by digital holographic microscopy.
abstract: Digital holographic microscopy provides new facilities for contactless and marker-free quantitative phase contrast imaging. In this work, a digital holographic microscopy method for the integral refractive index determination of living single cells in cell culture medium is presented. Further, the obtained refractive index information is applied to full field thickness and shape determination of adherent pancreas tumor cells, as well as for analysis of drug-induced dynamic changes of a single cell's cytoskeleton. The results demonstrate that digital holographic microscopy is a quantitative phase contrast technique for living cells under conventional laboratory conditions.
authors.affiliation: University of Muenster, Laboratory of Biophysics, Germany. bkemper@uni-muenster.de
authors.forename: Björn
authors.initials: B
authors.lastname: Kemper
authors.forename: Daniel
authors.initials: D
authors.lastname: Carl
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schnekenburger
authors.forename: Ilona
authors.initials: I
authors.lastname: Bredebusch
authors.forename: Marcus
authors.initials: M
authors.lastname: Schäfer
authors.forename: Wolfram
authors.initials: W
authors.lastname: Domschke
authors.forename: Gert
authors.initials: G
authors.lastname: von Bally
mesh_terms: D045744:Cell Line, Tumor; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods; D010190:Pancreatic Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D012031:Refractometry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*