title: Effects of dynamic compressive load on collagen-based scaffolds seeded with fibroblast-like synoviocytes.
abstract: Synoviocytes have been speculated to play potential reparative and remodeling roles in vascular meniscal injuries. In addition, synoviocytes may mediate the transformation of intraarticularly placed collagen-based scaffolds into fibrocartilage through exposure to dynamic compressive loads. The objectives of this study were to assess the feasibility of using fibroblast-like synoviocytes (FLS) to engineer meniscal-like fibrocartilage and to better understand the mechanosensitivity of FLS by seeding them onto collagen scaffolds exposed to dynamic compressive loads. Canine FLS were seeded onto disks of four commercially available collagen-based scaffolds (Restore, Permacol, Cuff Patch, and Graff Jacket) and subjected either to one of two levels of intermittent dynamic compressive load or no load. The disks were harvested at 1 and 2 weeks and assessed for cell viability, retention, and infiltration, as well as extracellular matrix production. In general, loading regimens decreased cellularity, and nonloaded Restore grafts retained the most cells across time intervals. Spatial distribution of FLS was optimized in Restore grafts and was overall better in non-crosslinked collagen scaffolds (Restore and Graft Jacket) than cross-linked matrices. Collagen production was noted in association with penetrating FLS clusters in the Restore scaffolds only. The applied biomechanical stimulus did not appear to induce fibrochondrogenesis in any treatment group. These data suggest that Restore scaffolds may foster greater cell retention and infiltration when compared to other commercially available, collagen-based biomatrices.
authors.affiliation: The Comparative Orthopaedic Laboratory, University of Missouri-Columbia, Columbia, Missouri 65211, USA. foxdb@missouri.edu
authors.forename: Derek B
authors.initials: DB
authors.lastname: Fox
authors.forename: James L
authors.initials: JL
authors.lastname: Cook
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kuroki
authors.forename: Mary
authors.initials: M
authors.lastname: Cockrell
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002465:Cell Movement / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen; D019245:Compressive Strength; D004285:Dogs; D005347:Fibroblasts / Q000166:cytology*; D008422:Materials Testing; D013583:Synovial Membrane / Q000166:cytology*; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D003094:Collagen