Transneuronal mapping of the CNS network controlling sympathetic outflow to the rat thymus.
The thymus is a primary immune organ that is essential for the development of functional T cells. The thymus receives sympathetic innervation, and thymocytes and thymic epithelial cells express functional adrenergic receptors. In this study, we employed retrograde, transneuronal virus tracing to identify the CNS cell groups that regulate sympathetic outflow to the thymus. Pseudorabies virus (PRV) was injected into the thymus, and the pattern of PRV infection in sympathetic regulatory centers of the CNS was determined at 72 and 120 h post-inoculation. PRV infection within the CNS first appeared within the spinal cord at 72 h post-inoculation and was confined to neurons within the intermediolateral cell column at levels T1-T7. At 120 h post-inoculation infection had spread within the spinal cord to include the central autonomic nucleus, intercalated cell nucleus and light infection within the cells of the lateral funiculus. Within the brain, PRV positive cells were found within nuclei of the medulla oblongata, pons and hypothalamus. Infection in the hypothalamus was observed within the arcuate nucleus, dorsal, lateral, and posterior hypothalamus and in all parvicellular subdivisions of the paraventricular hypothalamic nucleus. None of the infected animals exhibited labeling of the dorsal motor nucleus of the vagus. In summary, this study provides the first anatomic map of CNS neurons involved in control of sympathetic outflow to the thymus.
Trotter
R Nicholas
RN
Department of Microbiology, University of Virginia, PO Box 800734, Charlottesville, VA 22908, USA.
Stornetta
Ruth L
RL
Guyenet
Patrice G
PG
Roberts
Margo R
MR
D000818:Animals; D002452:Cell Count / Q000379:methods; D002490:Central Nervous System / Q000166:cytology* / Q000502:physiology* / Q000821:virology; D011558:Herpesvirus 1, Suid / Q000502:physiology; D008297:Male; D009434:Neural Pathways / Q000502:physiology* / Q000821:virology; D009474:Neurons / Q000502:physiology* / Q000821:virology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013950:Thymus Gland / Q000294:innervation* / Q000502:physiology*; D013997:Time Factors