Evaluation of models for the evolution of protein sequences and functions under structural constraint.
In the field of evolutionary structural genomics, methods are needed to evaluate why genomes evolved to contain the fold distributions that are observed. In order to study the effects of population dynamics in the evolved genomes we need fast and accurate evolutionary models which can analyze the effects of selection, drift and fixation of a protein sequence in a population that are grounded by physical parameters governing the folding and binding properties of the sequence. In this study, various knowledge-based, force field, and statistical methods for protein folding have been evaluated with four different folds: SH2 domains, SH3 domains, Globin-like, and Flavodoxin-like, to evaluate the speed and accuracy of the energy functions. Similarly, knowledge-based and force field methods have been used to predict ligand binding specificity in SH2 domain. To demonstrate the applicability of these methods, the dynamics of evolution of new binding capabilities by an SH2 domain is demonstrated.
Rastogi
Shruti
S
Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA.
Reuter
Nathalie
N
Liberles
David A
DA
D019295:Computational Biology / Q000379:methods*; D019020:Directed Molecular Evolution; D005418:Flavodoxin / Q000737:chemistry; D008954:Models, Biological; D017510:Protein Folding; D018909:src Homology Domains / Q000502:physiology*
D005418:Flavodoxin