Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate.
Two moderately halophilic low G + C Gram-positive bacteria were isolated from a sample of salted skate (Class Chondrychthyes, Genus Raja). Phylogenetic analysis of the 16S rRNA gene sequence of strains RH1(T) and RH4 showed that these organisms represented a novel species of the genus Salinicoccus. The new isolates formed pink-red colonies and flocculated in liquid media, with optimum growth in media containing 4% NaCl and pH of about 8.0. These organisms are aerobic but reduce nitrate to nitrite under anaerobic conditions. Acid is produced from several carbohydrates. Oxidase and catalase were detected. Menaquinone 6 was the major respiratory quinone. The major fatty acids of strains RH1(T) and RH4 were 15:0 anteiso and 15:0 iso. The G + C contents of DNA were 46.2 and 46.0 mol%, respectively. The peptidoglycan was of A3alpha L-Lys-Gly(5-6) type. On the basis of the phylogenetic analyses, physiological and biochemical characteristics, we suggest that strain RH1(T) (=LMG 22840 = CIP 108576) represents a new species of the genus Salinicoccus, for which we propose the name Salinicoccus salsiraiae.
França
Luís
L
Departamento de Bioquímica, Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3001-401 Coimbra, Portugal.
Rainey
Fred A
FA
Nobre
M Fernanda
MF
da Costa
Milton S
MS
D000818:Animals; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006093:Gram-Positive Asporogenous Rods / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D012689:Sequence Homology, Nucleic Acid; D012860:Skates, Fish / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*; D013030:Spain; D013696:Temperature; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000378:metabolism
D004269:DNA, Bacterial; D005227:Fatty Acids; D009566:Nitrates; D009573:Nitrites; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride; C042455:menaquinone 6; D010088:Oxidoreductases; D002374:Catalase