title: Production of deuterated lutein by Chlorella protothecoides and its detection by mass spectrometric methods.
abstract: Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the molecular mass cluster at around m/z 599. The rapidly growing microalga had much higher levels (58%) of deuterium substitution relative to previously reported (9-15%) natural sources of lutein.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine, 50 North Medical Drive, Moran Eye Center, Salt Lake City, UT 84132, USA.
authors.forename: Prakash
authors.initials: P
authors.lastname: Bhosale
authors.forename: Bogdan
authors.initials: B
authors.lastname: Serban
authors.forename: Paul S
authors.initials: PS
authors.lastname: Bernstein
mesh_terms: D002708:Chlorella / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003903:Deuterium; D014975:Lutein / Q000032:analysis / Q000096:biosynthesis*; D013058:Mass Spectrometry
chemical_list: D003903:Deuterium; D014975:Lutein