Visualization of the ribosomal DNA (45S rDNA) of Indica rice with FISH on some phases of cell cycle and extended DNA fibers.
The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza, sativa ssp. indica cv. Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity ofrDNA at individual loci may also vary through the cell cycle in rice. On extended DNA fibers, 45S rDNA signals appear as strings of numerous red spots, but some signals are missed in some regions, probably result from weak signals or intergenic spacers.
Li
Zong-Yun
ZY
School of Life Science, Xuzhou Normal University, Xuzhou 221116, China. zongyunli@yahoo.com.cn
Fu
Mei-Li
ML
Hu
Fang-Fang
FF
Huang
Shu-Feng
SF
Song
Yun-Chun
YC
D002453:Cell Cycle / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D012275:Oryza / Q000166:cytology* / Q000235:genetics*
D018744:DNA, Plant; D004275:DNA, Ribosomal