title: [Comparison of methods to construct a full-length cDNA library].
abstract: The use of full-length cDNA libraries is an effective tool to obtain complete gene information in a high-efficiency, high-throughput manner, especially in organisms with huge genomes that are not amenable to whole genome sequencing. In this review, we outlined several methods of full-length cDNA library construction and compared their advantages and disadvantages based on their respective principles. Drawing on our own experience, we described the Cap-trapper method in detail, with an emphasis on its application in wheat full-length cDNA library construction as well as the determination of the ratio of full-length cDNA in a library.
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Germplasm & Biotechnology/Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China. maoxg2001@sina.com
authors.forename: Xin-Guo
authors.initials: XG
authors.lastname: Mao
authors.forename: Rui-Lian
authors.initials: RL
authors.lastname: Jing
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Kong
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Zhao
authors.forename: Ji-Zeng
authors.initials: JZ
authors.lastname: Jia
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D015723:Gene Library