Molecular beacons for DNA binding proteins: an emerging technology for detection of DNA binding proteins and their ligands.
Quantitation of the level or activity of specific proteins is one of the most commonly performed experiments in biomedical research. Protein detection has historically been difficult to adapt to high throughput platforms because of heavy reliance upon antibodies for protein detection. Molecular beacons for DNA binding proteins is a recently developed technology that attempts to overcome such limitations. Protein detection is accomplished using inexpensive, easy-to-synthesize oligonucleotides, accompanied by a fluorescence readout. Importantly, detection of the protein and reporting of the signal occur simultaneously, allowing for one-step protocols and increased potential for use in high throughput analysis. While the initial iteration of the technology allowed only for the detection of sequence-specific DNA binding proteins, more recent adaptations allow for the possibility of development of beacons for any protein, independent of native DNA binding activity. Here, we discuss the development of the technology, the mechanism of the reaction, and recent improvements and modifications made to improve the assay in terms of sensitivity, potential for multiplexing, and broad applicability.
Dummitt
Benjamin
B
Mediomics, LLC, St. Louis, MO 63124, USA.
Chang
Yie-Hwa
YH
D001709:Biotechnology / Q000379:methods* / Q000639:trends; D004247:DNA / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D015336:Molecular Probe Techniques / Q000639:trends*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods* / Q000639:trends; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D004247:DNA