Concentrations of 15F2t isoprostane in urine of dogs with intervertebral disk disease.
OBJECTIVE
To measure 15F(2t) isoprostane concentrations in the urine of dogs undergoing ovariohysterectomy (OHE) and dogs undergoing surgery because of intervertebral disk disease (IVDD) and to assess relationships between urinary concentrations of 15F(2t) isoprostanes and neurologic score in dogs with IVDD.


METHODS
11 dogs undergoing OHE and 32 dogs with IVDD undergoing hemilaminectomy.


METHODS
Paired urine samples were obtained at induction of anesthesia and approximately 1 hour after OHE (controls) and were collected from dogs with IVDD at induction of anesthesia (28 samples) and approximately 1 hour after hemilaminectomy (31 samples); 26 paired urine samples were obtained from dogs with IVDD. Urinary isoprostane concentrations were measured by use of a commercial ELISA, and results were adjusted on the basis of urinary creatinine concentrations. Differences in the mean isoprostane-to-creatinine ratio were analyzed. Neurologic score was determined in dogs with IVDD by use of the modified Frankel scoring system.


RESULTS
Urinary isoprostane-to-creatinine ratios were significantly higher in dogs with IVDD than in control dogs before and after surgery. There was no significant difference between values before and after surgery for either group. There was a significant correlation of neurologic score and urinary isoprostane-to-creatinine ratio because dogs that had higher neurologic scores (ie, less severely affected) generally had higher isoprostane-to-creatinine ratios.


CONCLUSIONS
Urinary isoprostane-to-creatinine ratios were higher in dogs with IVDD before and after surgery. Analysis of these data suggests that dogs with IVDD are in a state of oxidative stress and that preemptive treatment with antioxidants warrants further investigation.
McMichael
Maureen A
MA
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station, 77843-4474, USA.
Ruaux
Craig G
CG
Baltzer
Wendy I
WI
Kerwin
Sharon C
SC
Hosgood
Giselle L
GL
Steiner
Jörg M
JM
Williams
David A
DA
D000818:Animals; D015415:Biomarkers / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000652:urine; D004283:Dog Diseases / Q000601:surgery / Q000652:urine*; D004285:Dogs; D005260:Female; D007405:Intervertebral Disc Displacement / Q000601:surgery / Q000652:urine / Q000662:veterinary*; D008297:Male
D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost