A one-step and sensitive GC-MS assay for meprobamate determination in emergency situations.
A rapid, sensitive, and ready-to-use gas chromatography-mass spectrometry method for meprobamate assay using carisoprodol as internal standard is described. The method involves extracting a 0.2-mL sample with chloroform, previously acidified with HCl 0.2N. For the quantitative analysis, we used selected-ion monitoring mode, selecting the ion m/z 144 for quantification of meprobamate and m/z 245 for carisoprodol. Excellent linearity was found between 0 and 200 mg/L plasma. The limit of detection was 0.58 mg/L, and the limit of quantification was 1.93 mg/L. A high reproducibility [intra-assay coefficient of variation (CV) range of 2.3-4.3% and interassay CV range of 5.5-12.3%] and accuracy (intra-assay range of 96.8-112.3% and interassay range of 85.5-99.3%) were observed. Recoveries were concentration-independent (87.0%, 76.2%, and 81.2% for 20, 75 and 150 mg/L, respectively). No interference from endogenous compounds, other metabolites of meprobamate, or frequently coadministered drugs was detected. This sensitive, simple assay for meprobamate in plasma, whole blood, and urine meets the current requirements for bioanalytical assays in overdose cases.
Daval
Sandrine
S
CHU Clermont-Ferrand, Laboratoire de Pharmacologie et Toxicologie, Hôpital G. Montpied, F-63003 Clermont-Ferrand, France.
Richard
Damien
D
Souweine
Bertrand
B
Eschalier
Alain
A
Coudore
François
F
D014151:Anti-Anxiety Agents / Q000097:blood / Q000652:urine; D001681:Biological Assay; D004635:Emergency Medicine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008620:Meprobamate / Q000097:blood* / Q000652:urine*; D009125:Muscle Relaxants, Central / Q000097:blood / Q000652:urine; D010949:Plasma / Q000737:chemistry
D014151:Anti-Anxiety Agents; D009125:Muscle Relaxants, Central; D008620:Meprobamate