title: Distribution of the functional MDR1 C3435T polymorphism in the Han population of China.
abstract: BACKGROUND
  3435C>T, a single nucleotide polymorphism (SNP) in exon 26 of the MDR1 gene, is linked to the variability of P-gp expression and function among different individuals. It was found that ethnic differences exist in the polymorphism of the MDR1(C3435T) gene. However, the distribution of 3435C>T genotypes in the Chinese Han population is not clear up till now.
  
  
  METHODS
  In this study, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was applied to assess 3435C>T genotypes in 265 healthy individuals of the Han population of China.
  
  
  RESULTS
  The genotype frequencies were: CC 32% (n = 85), CT 48% (n = 127) and TT 20% (n = 53). C and T allele frequencies were 0.56 and 0.44 respectively. The C allelic frequency for female was 56.5% (T: 43.5%), and 55.9% (T: 44.1%) for male.
  
  
  CONCLUSIONS
  There was no significant difference between females and males (p = 0.92, OR = 1.02, 95%CI: 0.67-1.57) in the present study. The frequency of C-allele was similar to that of some populations in Asia/Europe and was lower than that of populations in Africa. When compared with the study on Singapore-Chinese, some differences were found. The results of this study would be useful for individualised therapy of some diseases, and could have a prognostic implication for the Chinese Han population.
authors.affiliation: Lab of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics,Chinese Academy of Sciences, Dalian, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000145:classification / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D003710:Demography; D005260:Female; D005787:Gene Frequency; D019450:Genes, MDR / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D009154:Mutation; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length