title: Prediction of peritoneal metastasis in advanced gastric cancer by gene expression profiling of the primary site.
abstract: Peritoneal metastasis is the most common cause of tumour progression in advanced gastric cancer. Clinicopathological findings including cytologic examination of peritoneal lavage have been applied to assess the risk of peritoneal metastasis, but are sometimes inadequate for predicting peritoneal metastasis in individuals. Hence, we tried to construct a new prediction system for peritoneal metastasis by using a PCR-based high throughput array with 2304 genes. The prediction system, constructed from the learning set comprised of 30 patients with the most informative 18 genes, classified each case into a 'good signature group' or 'poor signature group'. Then, we confirmed the predictive performance in an additional validation set comprised of 24 patients, and the prediction accuracy for peritoneal metastasis was 75%. Kaplan-Meier analysis with peritoneal metastasis revealed significant difference between these two groups (P=0.0225). By combining our system with conventional clinicopathological factors, we can identify high risk cases for peritoneal metastasis more accurately.
authors.affiliation: Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka Suita, Osaka 565-0871, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Motoori
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takemasa
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Doki
authors.forename: Sakae
authors.initials: S
authors.lastname: Saito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyata
authors.forename: Shuji
authors.initials: S
authors.lastname: Takiguchi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: Takushi
authors.initials: T
authors.lastname: Yasuda
authors.forename: Masahiko
authors.initials: M
authors.lastname: Yano
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Kurokawa
authors.forename: Takamichi
authors.initials: T
authors.lastname: Komori
authors.forename: Makoto
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Noriko
authors.initials: N
authors.lastname: Ueno
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Oba
authors.forename: Shin
authors.initials: S
authors.lastname: Ishii
authors.forename: Morito
authors.initials: M
authors.lastname: Monden
authors.forename: Kikuya
authors.initials: K
authors.lastname: Kato
mesh_terms: D000368:Aged; D018572:Disease-Free Survival; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010534:Peritoneal Neoplasms / Q000556:secondary*; D011237:Predictive Value of Tests; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics*