Hyaluronan expression following middle cerebral artery occlusion in the rat.
Hyaluronic acid, a major component of the brain extracellular matrix, is a regulator of angiogenesis, cell differentiation and migration. We used the rat middle cerebral artery occlusion model to show hyaluronan accumulation in stroke-affected areas. Using reverse transcription-polymerase chain reaction and Western blotting we showed up-regulation of hyaluronidase-1 and 2 between 1 h and 21 days after stroke. Hyaluronidase-1 was up-regulated earlier than hyaluronidase-2. The hyaladherins, receptor for hyaluronan-mediated motility and CD44 were also increased after stroke. Using immunohistochemistry, we showed association of hyaluronidases 1/2 and hyaladherins with neurons in the infarcted and peri-infarcted regions and hyaluronidase-1 with microvessels. Hyaluronan synthesis and degradation in the stroke hemisphere might have an impact on neuronal survival, angiogenesis and general tissue remodelling after stroke.
Al Qteishat
Ahmed
A
The School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, UK.
Gaffney
John J
JJ
Krupinski
Jerzy
J
Slevin
Mark
M
D000818:Animals; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000502:physiology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006820:Hyaluronic Acid / Q000235:genetics*; D006821:Hyaluronoglucosaminidase / Q000235:genetics*; D020244:Infarction, Middle Cerebral Artery / Q000201:enzymology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase