title: Podocyte-associated proteins FAT, alpha-actinin-4 and filtrin are expressed in Langerhans islets of the pancreas.
abstract: Nephrin is a crucial podocyte molecule in the kidney glomerular filtration barrier and it is also expressed in Langerhans islet beta cells of the pancreas. Recently, genetic mapping of proteinuric kidney disease genes and animal models have revealed further important molecules for the kidney filtration function including alpha-actinin-4, podocin, FAT, and NEPH1. This study was addressed to explore the pancreatic expression of the podocyte molecules podocin, FAT, alpha-actinin-4, NEPH1, NEPH2, filtrin/NEPH3, synaptopodin and CD2 associated protein (CD2AP). The mRNA and protein expressions were studied by RT-PCR and immunoblotting, and localization in the pancreas was investigated by immunofluorescence. Of the nephrin-associated podocyte proteins, filtrin/NEPH3, FAT, and alpha-actinin-4 were found to be expressed in the pancreas at the gene and protein level and localized to Langerhans islets. Immunoreactivity with the podocin antibody was detected mostly in the exocrine pancreas. NEPH1 and synaptopodin expression was detected only at the mRNA level. Further studies are needed to unravel the functional role of these podocyte-associated molecules in the pancreatic Langerhans islets.
authors.affiliation: Research Program in Molecular Medicine, Biomedicum Helsinki, Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, PB 63, 00014, Helsinki, Finland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Rinta-Valkama
authors.forename: Tuula
authors.initials: T
authors.lastname: Palmén
authors.forename: Markus
authors.initials: M
authors.lastname: Lassila
authors.forename: Harry
authors.initials: H
authors.lastname: Holthöfer
mesh_terms: D000185:Actinin / Q000235:genetics / Q000378:metabolism*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D015151:Immunoblotting; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D007801:Langerhans Cells / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology*; D050199:Podocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015820:Cadherins; C502403:FAT1 protein, human; D007136:Immunoglobulins; C482646:KIRREL2 protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger; D000185:Actinin