title: Study of biological safety of scaffold material with heterologous deproteinized bone.
abstract: OBJECTIVE
  To evaluate the biological safety of manufactured heterologous deproteinized bone and to provide an experimental basis for clinical applications.
  
  
  METHODS
  Deproteinized bone (10 mm) and leaching liquor were made from pig ribs with a series of physical and chemical methods, then were evaluated through acute and subacute toxicity test, hemolysis test, pyrogen test, intracutaneous test, intramuscular implantation test and cytotoxity test.
  
  
  RESULTS
  No obvious toxicity, hemolysis, pyrogenic characteristics, skin irritation, inflammatory reaction after intramuscular implantation and cytotoxity were observed.
  
  
  CONCLUSIONS
  The heterologous deproteinized bone has good biological safety and meets all the demands of scaffold material for tissue engineering.
authors.affiliation: Center for Joint Surgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Kang-lai
authors.initials: KL
authors.lastname: Tang
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Yue-kui
authors.initials: YK
authors.lastname: Jing
authors.forename: Qi-hong
authors.initials: QH
authors.lastname: Li
mesh_terms: D000818:Animals; D016025:Bone Transplantation; D003601:Cytotoxicity Tests, Immunologic; D006461:Hemolysis; D051379:Mice; D011817:Rabbits; D023822:Tissue Engineering; D014183:Transplantation, Heterologous