Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococcus fascians.
In a search for novel immunostimulating substances we detected that culture supernatants of the gram-positive phytopathogenic bacterium, Rhodococcus fascians, were able to induce cytokine release (TNF(alpha)) from mouse peritoneal macrophages. Monoclonal antibodies were generated against the active principle, and were employed for its isolation and partial characterization as a high molecular (MW>100 kDa) glycoprotein. In addition, methods practicable for its biotechnological preparation and several ELISA variants for its determination were developed.
Butschak
Günter
G
Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany. uwe.karsten@glycotope.com
Karsten
Uwe
U
Schelhaas
Ute
U
Ott
Holger
H
Emmendörffer
Andreas
A
Niemeyer
Bernd
B
Helmholz
Heike
H
Goletz
Steffen
S
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D007155:Immunologic Factors / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D051379:Mice; D012240:Rhodococcus / Q000737:chemistry* / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006023:Glycoproteins; D007155:Immunologic Factors