The complete sequence and segregational stability analysis of a new cryptic plasmid pIGWZ12 from a clinical strain of Escherichia coli.
A new cryptic plasmid from a multi-resistant, multi-plasmid clinical strain of Escherichia coli has been isolated. The sequence of the 4072-base-pair pIGWZ12 (GenBank Accession No. DQ311641) was determined and analyzed. Two open-reading frames that code for proteins involved in plasmid mobilization and initiation of replication were identified. The putative origin of replication possesses all characteristic features of the theta mechanism for replicating plasmids. pIGWZ12 is stably maintained without selective pressure in bacterial cultures (for up to 80 generations), making it a good candidate for engineering a new cloning vector.
Zaleski
Piotr
P
Institute of Biotechnology and Antibiotics, Staroscinska 5, 02-516 Warsaw, Poland. zaleskip@iba.waw.pl
Wolinowska
Renata
R
Strzezek
Krystyna
K
Lakomy
Agata
A
Plucienniczak
Andrzej
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology