Isolation and characterization of Listeria monocytogenes isolates from ready-to-eat foods in Florida.
Of 3,063 ready-to-eat food samples tested, 91 (2.97%) were positive for Listeria monocytogenes, and lineage 1 strains outnumbered lineage 2 strains 57 to 34. Seventy-one isolates (78%) exhibited multiple antibiotic resistance, and an L. monocytogenes-specific bacteriophage cocktail lysed 65 of 91 (71%) isolates. Determining phage, acid, and antibiotic susceptibility phenotypes enabled us to identify differences among strains which were otherwise indistinguishable by conventional methods.
Shen
Yuelian
Y
Florida State Department of Agriculture and Consumer Services, Tallahassee, Florida, USA.
Liu
Yan
Y
Zhang
Yifan
Y
Cripe
Jennifer
J
Conway
William
W
Meng
Jianghong
J
Hall
Grace
G
Bhagwat
Arvind A
AA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001435:Bacteriophages / Q000502:physiology; D016521:Electrophoresis, Gel, Pulsed-Field; D005431:Florida; D005506:Food Contamination; D005511:Food Handling / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification* / Q000821:virology; D008242:Lysogeny; D008461:Meat Products / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D012474:Salmon / Q000382:microbiology*; D013997:Time Factors
D000900:Anti-Bacterial Agents