Characteristics of the adhesion of PCC Lactobacillus fermentum VRI 003 to Peyer's patches.
The characteristics of the adhesion of PCC Lactobacillus fermentum VRI 003 to Peyer's patches was studied in vitro. The adhesion of L. fermentum 003 was strongly inhibited in the presence of d-mannose and methyl-alpha-d-mannoside although other carbohydrates tested, such as N-acetyl-glucosamine, d-galactose, d-glucose and l-fucose, did not affect the adhesion. Lactobacillus fermentum 003 was shown to strongly attach to mannose immobilized on a surface using BSA, suggesting that L. fermentum 003 specifically adhered to mannose-containing molecule(s). Pretreatment of L. fermentum 003 with proteinase K and trypsin decreased the adhesive capacity and bacterial surface extracts diminished adhesion of L. fermentum 003 indicating that cell surface proteins are involved in adhesion to Peyer's patches. It was concluded that a mannose-specific protein mediated adhesion of L. fermentum 003 to the Peyer's patches.
Kang
Seok-Seong
SS
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, Australia.
Conway
Patricia L
PL
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D002241:Carbohydrates / Q000494:pharmacology; D019286:Endopeptidase K / Q000494:pharmacology; D005260:Female; D007413:Intestinal Mucosa / Q000382:microbiology; D052197:Limosilactobacillus fermentum / Q000187:drug effects / Q000502:physiology*; D008358:Mannose / Q000494:pharmacology; D008565:Membrane Proteins / Q000502:physiology; D008766:Methylmannosides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000382:microbiology*
D002241:Carbohydrates; D008565:Membrane Proteins; D008766:Methylmannosides; C008466:methylmannoside; D019286:Endopeptidase K; D008358:Mannose