title: Production of protective antibodies against Porphyromonas gingivalis strains by immunization with recombinant gingipain domains.
abstract: We studied the effect of antibodies against Porphyromonas gingivalis gingipain domains, preparing them against three recombinant fragments of RgpA (catalytic domain, r-Rgp CAT; hemagglutinin domains, r-Rgp 44 and r-Rgps 15-27) and one fragment of Kgp (catalytic domain, r-Kgp CAT). Enhancement of opsonization and killing by human polymorphonuclear leukocytes were measured in the noninvasive FDC 381 and invasive W50 strains of P. gingivalis. Anti-r-Rgp 44 was the most effective in both strains of P. gingivalis. The present findings lead us to recommend RgpA 44 as a candidate immunogen for vaccines against P. gingivalis.
authors.affiliation: Department of Periodontics, Tokyo Dental College, Chiba, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Yasaki-Inagaki
authors.forename: Satoru
authors.initials: S
authors.lastname: Inagaki
authors.forename: Satoru
authors.initials: S
authors.lastname: Yamada
authors.forename: Katsuji
authors.initials: K
authors.lastname: Okuda
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Ishihara
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000276:immunology* / Q000378:metabolism; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D001770:Blood Bactericidal Activity / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins / Q000276:immunology; D049449:Luminescence; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000276:immunology*; D017434:Protein Structure, Tertiary; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D009895:Opsonin Proteins; D014614:Vaccines, Synthetic; D003546:Cysteine Endopeptidases