Role of free radicals on mechanism of radiation nephropathy.
OBJECTIVE
In our study, after applying a single dose of 612 cGy irradiation, we aimed to observe the role of free radicals on tissue damage in the kidney caused by radiation by measuring NO level, Na/K-ATPase activity and TBARS amount which is an indicator of free radical damage. On the other hand we investigated whether the tissue damage can be prevented by vitamin A or not.


METHODS
This study was performed on three groups: 1. Control group 2. The group to which irradiation was administrated 3. The group which was given radiation + vitamin A. The irradiation group of animals were given a single dose of gamma irradiation at a sublethal dose. In the group which was administrated both irradiation + vitamin A, vitamin A was given for two days prior to irradiation. The amount of NO was measured by ESR spectroscopy, Na/K-ATPase and TBARS were measured by spectrophotometry.


CONCLUSIONS
As a result of radiation mediated tissue damage in the kidney, we observed a NO loss, a decrease in Na/K-ATPase activity and an increase in TBARS amount. Although the administration of vitamin A before radiation, did not have any effect on NO loss and decrease in Na/K-ATPase.
Balabanli
B
B
Department of Biology, Gazi University Faculty of Arts & Science, 06500 Teknikokullar, Ankara, Turkey. bbalabanli@gazi.edu.tr
Türközkan
N
N
Akmansu
M
M
Polat
M
M
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000378:metabolism*; D005720:Gamma Rays; D006168:Guinea Pigs; D007668:Kidney / Q000378:metabolism / Q000528:radiation effects; D007674:Kidney Diseases / Q000209:etiology*; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000209:etiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014801:Vitamin A / Q000494:pharmacology
D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D014801:Vitamin A; D009569:Nitric Oxide; D000254:Sodium-Potassium-Exchanging ATPase