title: Study of laser created ZRO2 and hydroxyapatite/ZrO2 films for implantology.
abstract: Thin films of ZrO2 and hydroxyapatite/ZrO2 were created by excimer laser ablation on Ti6Al4V substrates. ZrO2 layers were fabricated in vacuum by KrF laser at various substrate temperatures and hydroxyapatite (HA) layers were fabricated in water vapor ambient by ArF laser and in water vapor/argon ambient by KrF excimer laser. Film properties were evaluated by XRD, SEM and WDX methods. The test of mechanical adhesion was proceeded on ZrO2 films. XRD analysis proved the presence of amorphous or crystalline HA in the deposited films. SEM method demonstrated smooth surface covered by droplets for both HA and ZrO2 films. Ca/P ratio of the HA films is higher than that of the natural HA and is within the range of 2.8-3.0. The HA/ZrO2 and ZrO2 samples were tested in vitro for cytotoxicity. The best results were received by the HA/ZrO2 samples in the test of cytotoxicity. Fibroblasts cultivating with HA/ZrO2 samples exhibited subconfluent and confluent growth and showed fibronectin homogenously.
authors.affiliation: Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, 182 21 Prague 8, Czech Republic. jelinek@fzu.cz
authors.forename: M
authors.initials: M
authors.lastname: Jelínek
authors.forename: T
authors.initials: T
authors.lastname: Dostálová
authors.forename: Z
authors.initials: Z
authors.lastname: Teuberová
authors.forename: M
authors.initials: M
authors.lastname: Seydlová
authors.forename: P
authors.initials: P
authors.lastname: Masínová
authors.forename: T
authors.initials: T
authors.lastname: Kocourek
authors.forename: W
authors.initials: W
authors.lastname: Mróz
authors.forename: A
authors.initials: A
authors.lastname: Prokopiuk
authors.forename: K
authors.initials: K
authors.lastname: Smetana
mesh_terms: D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry* / Q000528:radiation effects; D006244:Hardness; D007834:Lasers; D008422:Materials Testing; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D019736:Prostheses and Implants; D013499:Surface Properties; D015040:Zirconium / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D017886:Durapatite; D015040:Zirconium; C028541:zirconium oxide