The 3'-UTR C>T polymorphism of the oxidized LDL-receptor 1 (OLR1) gene does not associate with coronary artery disease in Italian CAD patients or with the severity of coronary disease.
OBJECTIVE
Oxidized low-density lipoproteins (OxLDLs) play a critical role in endothelial dysfunction, which is implicated in the pathogenesis of atherosclerosis. Vascular endothelial cells internalize and degrade oxLDL through the endothelial lectin-like oxidized LDL receptor 1 (OLR1). OLR1 is up-regulated in several pathological conditions, including hypertension, hyperlipidemia, diabetes, atherosclerosis and inflammation, and represents therefore a good candidate for coronary artery disease (CAD). Recently, a 3'-UTR (188 C>T) SNP in the OLR1 gene has been reported to be associated with coronary artery stenosis and myocardial infarction. In the present study we investigated whether the OLR1 gene 188 C>T SNP is a genetic risk marker for CAD in Italian patients with angiographically defined coronary atherosclerosis, and assessed its relation with clinical and metabolic abnormalities, including severity of disease (classified as restenosis, single- or multiple coronary vessels disease, and MI).


METHODS
The 3'-UTR C>T SNP was detected in real-time PCR in 351 subjects with CAD and in 215 control subjects.


RESULTS
The OLR1-T allele frequencies were 48.9% in the CAD subjects and 47.7% in controls, with no significant difference between the two groups. Also, the 3'-UTR C>T SNP did not associate with any of the parameters of severity of disease. Furthermore, none of the other clinical and metabolic parameters were associated with the OLR1 gene SNP.


CONCLUSIONS
Our observations suggest that, in our population, the 3'-UTR C>T polymorphism of the OLR1 gene is unlikely to play a role in the pathogenesis of coronary artery disease.
Sentinelli
Federica
F
Department of Clinical Sciences, Institute of Clinical Medicine, Division of Endocrinology, University of Rome La Sapienza, Rome, Italy.
Filippi
Emanuela
E
Fallarino
Mara
M
Romeo
Stefano
S
Fanelli
Marzia
M
Buzzetti
Raffaella
R
Berni
Andrea
A
Baroni
Marco G
MG
D020413:3' Untranslated Regions / Q000235:genetics*; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism; D051131:Receptors, Oxidized LDL; D051127:Scavenger Receptors, Class E; D012720:Severity of Illness Index
D020413:3' Untranslated Regions; D005819:Genetic Markers; C495137:OLR1 protein, human; D011973:Receptors, LDL; D051131:Receptors, Oxidized LDL; D051127:Scavenger Receptors, Class E