title: Toxicity assessment of reference and natural freshwater sediments with the LuminoTox assay.
abstract: We examined the possibility of adapting the LuminoTox, a recently-commercialized bioanalytical testing procedure initially developed for aqueous samples, to assess the toxic potential of sediments. This portable fluorescent biosensor uses photosynthetic enzyme complexes (PECs) to rapidly measure photosynthetic efficiency. LuminoTox testing of 14 CRM (Certified Reference Material) sediments was first undertaken with (1) a "solid phase assay" (Lum-SPA) in which PECs are in intimate contact with sediment slurries for a 15 min exposure period and (2) an elutriate assay (Lum-ELU) in which PECs are exposed for 15 min to sediment water elutriates. CRM sediment toxicity data were then compared with those generated with the Microtox Solid Phase Assay (Mic-SPA). A significant correlation (P < 0.05) was shown to exist between Lum-SPA and Mic-SPA, indicating that both tests display a similar toxicity response pattern for CRM sediments having differing contaminant profiles. The sediment elutriate Lum-ELU assay displayed toxicity responses (i.e. measurable IC20s) for eight of the 14 CRM sediments, suggesting that it is capable of determining the presence of sediment contaminants that are readily soluble in an aqueous elutriate. Lum-SPA and Mic-SPA bioassays were further conducted on 12 natural freshwater sediments and their toxicity responses were more weakly, yet significantly, correlated. Finally, Lum-SPA testing undertaken with increasing mixtures of kaolin clay confirmed that its toxicity responses, in a manner similar to those reported for the Mic-SPA assay, are also subject to the influence of grain size. While further studies will be required to more fully understand the relationship between Lum-SPA assay responses and the physicochemical makeup of sediments (e.g., grain size, combined presence of natural and anthropogenic contaminants), these preliminary results suggest that LuminoTox testing could be a useful screen to assess the toxic potential of solid media.
authors.affiliation: Laboratório de Ecotoxicologia, Centro de Energia Nuclear na Agricultura, Piracicaba-SP, Brazil.
authors.forename: P M
authors.initials: PM
authors.lastname: Dellamatrice
authors.forename: R T R
authors.initials: RT
authors.lastname: Monteiro
authors.forename: C
authors.initials: C
authors.lastname: Blaise
authors.forename: J L
authors.initials: JL
authors.lastname: Slabbert
authors.forename: F
authors.initials: F
authors.lastname: Gagné
authors.forename: S
authors.initials: S
authors.lastname: Alleau
mesh_terms: D048908:Aliivibrio fischeri / Q000187:drug effects; D001681:Biological Assay / Q000379:methods; D005618:Fresh Water / Q000737:chemistry*; D019015:Geologic Sediments; D010316:Particle Size; D012995:Solubility; D018675:Toxicity Tests / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical