title: Transposon-free insertions for insect genetic engineering.
abstract: Methods involving the release of transgenic insects in the field hold great promise for controlling vector-borne diseases and agricultural pests. Insect transformation depends on nonautonomous transposable elements as gene vectors. The resulting insertions are stable in the absence of suitable transposase, however, such absence cannot always be guaranteed. We describe a method for post-integration elimination of all transposon sequences in the pest insect Medfly, Ceratitis capitata. The resulting insertions lack transposon sequences and are therefore impervious to transposase activity.
authors.affiliation: Oxitec Limited, 71 Milton Park, Oxford OX14 4RX, UK.
authors.forename: Tarig H
authors.initials: TH
authors.lastname: Dafa'alla
authors.forename: George C
authors.initials: GC
authors.lastname: Condon
authors.forename: Kirsty C
authors.initials: KC
authors.lastname: Condon
authors.forename: Caroline E
authors.initials: CE
authors.lastname: Phillips
authors.forename: Neil I
authors.initials: NI
authors.lastname: Morrison
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Epton
authors.forename: Guoliang
authors.initials: G
authors.lastname: Fu
authors.forename: Luke
authors.initials: L
authors.lastname: Alphey
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D033622:Ceratitis capitata / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D017353:Gene Deletion; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics*; D042822:Genomic Instability / Q000235:genetics*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D019895:Transposases / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D019895:Transposases