title: Reliable on-line sample preparation of basic compounds from plasma using a reversed phase restricted access media in column-switching LC.
abstract: We investigated on-line sample preparation of basic compounds from plasma using a methylcellulose-immobilized reversed-phase restricted-access media in column-switching liquid chromatography (LC). Dilution of the plasma sample with phosphate buffered saline prevented or delayed the formation of fibrin clots at 4 degrees C and resulted in reproducible on-line sample preparation over a 30-h period. The use of an ion-pair reagent in the extraction LC enhanced recoveries of hydrophilic basic compounds. The ability of the methods to quantify compounds in plasma were validated and the method was successfully applied to the pharmacokinetic study of a hydrophilic basic compound injected into the bloodstream of rats.
authors.affiliation: Analytical Research Laboratories, Eisai Co. Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan. e-yamamoto@hhc.eisai.co.jp
authors.forename: Eiichi
authors.initials: E
authors.lastname: Yamamoto
authors.forename: Hatsue
authors.initials: H
authors.lastname: Igarashi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sato
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kushida
authors.forename: Takashi
authors.initials: T
authors.lastname: Kato
authors.forename: Takashi
authors.initials: T
authors.lastname: Kajima
authors.forename: Naoki
authors.initials: N
authors.lastname: Asakawa
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015195:Drug Design; D005466:Fluorocarbons / Q000737:chemistry; D007275:Injections, Intravenous; D008297:Male; D008747:Methylcellulose / Q000737:chemistry; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000097:blood* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000097:Acetonitriles; D005466:Fluorocarbons; D004364:Pharmaceutical Preparations; C033094:perfluorobutyric acid; D008747:Methylcellulose; C032159:acetonitrile