[Synchronous fluorescence and raman spectroscopy study on the interaction of pulsed electric field (PEF) and bovine serum albumin (BSA)].
The interaction of pulsed electric field (PEF) and bovine serum albumin (BSA) was studied by synchronous fluorescence and Raman spectroscopy. The results of synchronous fluorescence showed that pulsed electric field exerted its effects on the emission fluorescence spectrum and reduced the fluorescence intensities of the tyrosine and tryptophan side chains. The results of Raman spectroscopy verified this. These two experiments indicated that PEF exposure changed the microenvironments of the two aromatic amino adds, which were located in the active parts of BSA, and further indicated the conformational changes of the proteins, and the change in its biological functions.
Li
Le-jun
LJ
Key Laboratory of Optical and Magnetic Resonance Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062, China.
Chen
Shu-de
SD
Qiao
Deng-jiang
DJ
D000818:Animals; D002417:Cattle; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D012710:Serum Albumin, Bovine