Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation.
C-type lectins are the most diverse and prevalent lectin family in immunity. Particular interest has recently been attracted by the C-type lectin-like receptors on NK cells, which appear to regulate the activation/inhibitory balance of these cells, controlling cytotoxicity and cytokine production. We previously identified a human C-type lectin-like receptor, closely related to both the beta-glucan receptor and the lectin-like receptor for oxidized-LDL, named MICL (myeloid inhibitory C-type lectin-like receptor), which we had shown using chimeric analysis to function as an inhibitory receptor. Using a novel MICL-specific monoclonal antibody, we show here that human MICL is expressed primarily on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells. Although MICL was highly N-glycosylated in primary cells, the level of glycosylation was found to vary between cell types. MICL surface expression was down-regulated during inflammatory/activation conditions in vitro, as well as during an in vivo model of acute inflammation, which we characterize here. This suggests that human MICL may be involved in the control of myeloid cell activation during inflammation.
Marshall
Andrew S J
AS
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
Willment
Janet A
JA
Pyz
Elwira
E
Dennehy
Kevin M
KM
Reid
Delyth M
DM
Dri
Pietro
P
Gordon
Siamon
S
Wong
Simon Y C
SY
Brown
Gordon D
GD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D015536:Down-Regulation; D006031:Glycosylation; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D011975:Receptors, Mitogen / Q000378:metabolism*
D000911:Antibodies, Monoclonal; C485220:CLEC12A protein, human; D037181:Lectins, C-Type; D011975:Receptors, Mitogen