title: Molecular characterization of the Tim9 homologue from the methylotrophic yeast Pichia methanolica.
abstract: In this paper we describe molecular characterization of the TIM9 gene encoding the essential mitochondrial inner-membrane protein in the methylotrophic yeast Pichia methanolica. PmTIM9 contains two exons corresponding to a gene product of 89 amino acid residues and a 140 bp intron. The deduced amino acid sequence exhibited high identity to those of other yeast Tim9ps, and possessed two CX(3)C motifs that contained two cysteine residues conserved among small Tim family proteins. Moreover, PmTIM9 had the ability to partially suppress the temperature sensitivity of Saccharomyces cerevisiae strain tim9-3, suggesting that PmTIM9 is a functional homologue of the ScTIM9 gene.
authors.affiliation: Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099-2493, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Matsufuji
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
authors.forename: Shuki
authors.initials: S
authors.lastname: Fujimura
authors.forename: Mika
authors.initials: M
authors.lastname: Chikui
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Miyaji
authors.forename: Noboru
authors.initials: N
authors.lastname: Tomizuka
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D008565:Membrane Proteins; D024101:Mitochondrial Proteins