title: Biochemical and molecular characterization of a lipase produced by Rhizopus oryzae.
abstract: A novel strain of Rhizopus oryzae WPG secretes a noninduced lipase (ROLw) in the culture medium; purified ROLw is a protein of 29 kDa, the 45 N-terminal amino acid residues were sequenced, this sequence is very homologous to Rhizopus delemar lipase (RDL), Rhizopus niveus lipase (RNL) and R. oryzae lipase (ROL29) sequences; the cloning and sequencing of the part of the gene encoding the mature ROLw, shows two nucleotides differences with RDL, RNL and ROL29 sequences corresponding to the change of the residues 134 and 200; ROLw does not present the interfacial activation phenomenon when using tripropionin or vinyl propionate as substrates; the lipase activity is maximal at pH 8 and at 37 degrees C, specific activities of 3500 or 900 U mg(-1) were measured at 37 degrees C and at pH 8, using olive oil emulsion or tributyrin as substrates, respectively; ROLw is unable to hydrolyse triacylglycerols in the presence of high concentration of bile salts; it is a serine enzyme as it is inhibited by tetrahydrolipstatin and was stable between pH 5 and pH 8.
authors.affiliation: Laboratoire de Biochimie, ENIS, Route de Soukra, BPW, Sfax, Tunisie.
authors.forename: Riadh Ben
authors.initials: RB
authors.lastname: Salah
authors.forename: Habib
authors.initials: H
authors.lastname: Mosbah
authors.forename: Ahmed
authors.initials: A
authors.lastname: Fendri
authors.forename: Ali
authors.initials: A
authors.lastname: Gargouri
authors.forename: Youssef
authors.initials: Y
authors.lastname: Gargouri
authors.forename: Hafedh
authors.initials: H
authors.lastname: Mejdoub
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004789:Enzyme Activation; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D012233:Rhizopus / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D013329:Structure-Activity Relationship; D014280:Triglycerides / Q000494:pharmacology
chemical_list: D014280:Triglycerides; D008049:Lipase; C031627:tripropionin