title: Flow injection amperometric determination of procaine in pharmaceutical formulation using a screen-printed carbon electrode.
abstract: A rapid and simple method for procaine determination was developed by flow injection analysis (FIA) using a screen-printed carbon electrode (SPCE) as amperometric detector. The present method is based on the amine/hydroxylamine oxidation from procaine monitored at 0.80 V on SPCE in sodium acetate solution pH 6.0. Using the best experimental conditions assigned as: pH 6.0, flow rate of 3.8 mL min(-1), sample volume of 100 microL and analytical path of 30 cm it is possible to construct a linear calibration curve from 9.0x10(-6) to 1.0x10(-4) mol L(-1). The relative standard deviation for 5.0x10(-5) mol L(-1) procaine (15 repetitions using the same electrode) is 3.2% and detection limit calculated is 6.0x10(-6) mol L(-1). Recoveries obtained for procaine gave a mean values from 94.8 to 102.3% and an analytical frequency of 36 injections per hour was achieved. The method was successfully applied for the determination of procaine in pharmaceutical formulation without any pre-treatment, which are in good accordance with the declared values of manufacturer and an official method based on spectrophotometric analysis.
authors.affiliation: Departamento de Química Analítica, Instituto de Química, Universidade Estadual Paulista, UNESP, CP 355, CEP 14801-970 Araraquara, SP, Brazil.
authors.forename: Márcio F
authors.initials: MF
authors.lastname: Bergamini
authors.forename: André L
authors.initials: AL
authors.lastname: Santos
authors.forename: Nelson R
authors.initials: NR
authors.lastname: Stradiotto
authors.forename: Maria Valnice B
authors.initials: MV
authors.lastname: Zanoni
mesh_terms: D000779:Anesthetics, Local / Q000032:analysis*; D002138:Calibration; D002244:Carbon; D002626:Chemistry, Pharmaceutical; D004563:Electrochemistry; D004566:Electrodes; D017022:Flow Injection Analysis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D011343:Procaine / Q000032:analysis*; D012996:Solutions
chemical_list: D000779:Anesthetics, Local; D007202:Indicators and Reagents; D012996:Solutions; D011343:Procaine; D002244:Carbon