title: HoxB domain induction silences DNA replication origins in the locus and specifies a single origin at its boundary.
abstract: In multicellular organisms, changes in the DNA replication programme could act to integrate differentiation with cell division in various developmental and transcriptional contexts. Here, we have addressed the use of DNA replication origins during differentiation in the HoxB domain-a cluster of nine genes developmentally regulated in a collinear manner. In undifferentiated mouse P19 cells, we detected several DNA replication origins in the 100 kb HoxB locus, indicating a relaxed origin use when the locus is transcriptionally silent. By contrast, in retinoic-acid-induced differentiated cells, when HoxB transcription is activated, a general silencing of DNA replication origins occurs in the locus except one located downstream of Hoxb1, at the 3' boundary of the HoxB domain. Silencing of the replication origins is associated with histone hyperacetylation, whereas the active Hoxb1 origin persists as a hypoacetylated island. These findings provide direct evidence for the differentiated use of origins in HoxB genes, and we suggest that this regulation might contribute to the regulated expression of HoxB genes during development.
authors.affiliation: Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Grégoire
authors.forename: Konstantin
authors.initials: K
authors.lastname: Brodolin
authors.forename: Marcel
authors.initials: M
authors.lastname: Méchali
mesh_terms: D000107:Acetylation; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D004261:DNA Replication / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D051738:Origin Recognition Complex / Q000235:genetics / Q000378:metabolism; D018741:Replication Origin / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D006657:Histones; D018398:Homeodomain Proteins; D051738:Origin Recognition Complex; D014212:Tretinoin