title: Simultaneous saccharification and co-fermentation of glucose and xylose in steam-pretreated corn stover at high fiber content with Saccharomyces cerevisiae TMB3400.
abstract: The two main sugars in the agricultural by-product corn stover are glucose and xylose. Co-fermentation of glucose and xylose at high content of water-insoluble solids (WIS) without detoxification is a prerequisite to obtain high ethanol concentration and to reduce production costs. A recombinant strain of Saccharomyces cerevisiae, TMB3400, was used in simultaneous saccharification and fermentation (SSF) of whole pretreated slurry of corn stover at high WIS. TMB3400 co-fermented glucose and xylose with relatively high ethanol yields giving high final ethanol concentration. The ethanol productivity increased with increasing concentration of pretreatment hydrolysate in the yeast production medium and when SSF was performed in a fed-batch mode.
authors.affiliation: Department of Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Ohgren
authors.forename: Oskar
authors.initials: O
authors.lastname: Bengtsson
authors.forename: Marie F
authors.initials: MF
authors.lastname: Gorwa-Grauslund
authors.forename: Mats
authors.initials: M
authors.lastname: Galbe
authors.forename: Bärbel
authors.initials: B
authors.lastname: Hahn-Hägerdal
authors.forename: Guido
authors.initials: G
authors.lastname: Zacchi
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods; D050260:Carbohydrate Metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013227:Steam; D014994:Xylose / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D013227:Steam; D000431:Ethanol; D014994:Xylose; D005947:Glucose