title: Morphogenesis of dermal-epidermal junction in a model of reconstructed skin: beneficial effects of vitamin C.
abstract: In skin, cohesion between the dermis and the epidermis is ensured by the dermal-epidermal junction which is also required for control of epidermal growth and differentiation. Here we showed that addition of vitamin C optimized the formation of the dermal-epidermal junction in an in vitro human reconstructed skin model leading to a structure closer to that of normal human skin. Compared with controls, vitamin C treatment led to a better organization of basal keratinocytes, an increase in fibroblast number and a faster formation of the dermal-epidermal junction. Vitamin C also accelerated deposition of several basement membrane proteins, like type IV and VII collagens, nidogen, laminin 10/11, procollagens I and III, tenascin C and fibrillin-1 at the dermal-epidermal junction. The mechanism of action of vitamin C was investigated by quantitative polymerase chain reaction in fibroblasts and keratinocytes respectively. Vitamin C effects passed in part through an increase in col I alpha1, col III alpha1 and fibrillin-1 mRNA levels. Effects on the other markers appeared to happen at the translational and/or post-translational level, as illustrated for tenascin C, col IV alpha2 and col VII alpha1 mRNA levels which were reduced by vitamin C in both cell types.
authors.affiliation: L'Oréal, Centre de Recherche, Clichy Cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Marionnet
authors.forename: Corinne
authors.initials: C
authors.lastname: Vioux-Chagnoleau
authors.forename: Cécile
authors.initials: C
authors.lastname: Pierrard
authors.forename: Juliette
authors.initials: J
authors.lastname: Sok
authors.forename: Daniel
authors.initials: D
authors.lastname: Asselineau
authors.forename: Françoise
authors.initials: F
authors.lastname: Bernerd
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001205:Ascorbic Acid / Q000627:therapeutic use*; D020405:Dermis / Q000187:drug effects / Q000502:physiology*; D004817:Epidermis / Q000187:drug effects / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D046529:Microscopy, Electron, Transmission; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012867:Skin / Q000187:drug effects*; D012879:Skin Physiological Phenomena
chemical_list: D001205:Ascorbic Acid