title: T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries.
abstract: We investigated whether the T7 system of phage display could produce peptide libraries of greater diversity than the M13 system of phage display due to the differing processes of lytic and filamentous phage morphogenesis. Using a bioinformatics-assisted computational approach, collections of random peptide sequences obtained from a T7 12-mer library (X(12)) and a T7 7-mer disulfide-constrained library (CX(7)C) were analyzed and compared with peptide populations obtained from New England BioLabs' M13 Ph.D.-12 and Ph.D.-C7C libraries. Based on this analysis, peptide libraries constructed with the T7 system have fewer amino acid biases, increased peptide diversity, and more normal distributions of peptide net charge and hydropathy than the M13 libraries. The greater diversity of T7-displayed libraries provides a potential resource of novel binding peptides for new as well as previously studied molecular targets. To demonstrate their utility, several of the T7-displayed peptide libraries were screened for streptavidin- and neutravidin-binding phage. Novel binding motifs were identified for each protein.
authors.affiliation: Basic Research Program, SAIC-Frederick, Inc., Frederick, MD, USA.
authors.forename: Lauren R H
authors.initials: LR
authors.lastname: Krumpe
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Atkinson
authors.forename: Gary W
authors.initials: GW
authors.lastname: Smythers
authors.forename: Andrea
authors.initials: A
authors.lastname: Kandel
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Schumacher
authors.forename: James B
authors.initials: JB
authors.lastname: McMahon
authors.forename: Lee
authors.initials: L
authors.lastname: Makowski
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Mori
mesh_terms: D000595:Amino Acid Sequence; D001360:Avidin / Q000737:chemistry; D017104:Bacteriophage M13 / Q000235:genetics*; D017123:Bacteriophage T7 / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods; D019809:Streptavidin / Q000737:chemistry
chemical_list: D019151:Peptide Library; D010455:Peptides; C093483:neutravidin; D001360:Avidin; D019809:Streptavidin