title: [Study on purification of 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside from Polygonum multiflori].
abstract: OBJECTIVE
  To study the conditions and parameters of purifying 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside from Polygonum multiflori.
  
  
  METHODS
  Absorption capacity of four resins for 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside was compared. With the adsorption ability as indexes, the process of absorbing and purifying 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside from P. multiflori with S-8 macroporous resin absorbent was selected by orthogonal design.
  
  
  RESULTS
  The S-8 resin was the best of the four resins. The optimum process condition was 50% ethanol as eluting solvent, the flow rate at 1.5 mL x min(-1), pH at 7-8, and the solution concentration at 0.2 g x mL(-1). The absorption capacity by this process was 36.89 mg x g(-1).
  
  
  CONCLUSIONS
  The process is simple and convenient and the regeneration of resin is easy, so this method of purification is advisable.
authors.affiliation: School of Biological and Environmental Engineering, Jiangsu University, Zhenjiang, China.
authors.forename: Tao-tao
authors.initials: TT
authors.lastname: Jiang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ouyang
authors.forename: Ya-dong
authors.initials: YD
authors.lastname: Miao
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Zhang
mesh_terms: D000042:Absorption; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D012117:Resins, Synthetic; D013267:Stilbenes