Establishment and characterization of a new cell line, FRTK-1, derived from human malignant rhabdoid tumor of the kidney, with overexpression of epidermal growth factor receptor and cyclooxygenase-2.
A new human malignant rhabdoid tumor (MRT) cell line (designated FRTK-1) was established from MRT of the kidney of an 18-month-old boy. The cell line is maintained for over 24 months with more than 100 passages. FRTK-1 cells in vitro showed 2 different growth patterns, adherent and non-adherent patterns. The FRTK-1 cells showed the same morphological and immunophenotypical characteristics as primary tumor cells of kidney. Cytogenetic and molecular analyses revealed a non-sense mutation in the hSNF5/INI1 gene and loss of expression of hSNF5/INI1 gene product protein. Epidermal growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2) were expressed in the FRTK-1 cells. Until now, there has been no report of MRT cell lines with expression of COX-2. Therefore, FRTK-1 cell line might be useful for investigating biological behavior and developing new molecular targeting antitumor drugs for MRT with expression of EGFR or COX-2.
Hakozaki
Michiyuki
M
First Department of Pathology, Fukushima Medical University School of Medicine, Fukushima-shi, Japan. paco@fmu.ac.jp
Hojo
Hiroshi
H
Sato
Michiko
M
Kaneko
Yasuhiko
Y
Watanabe
Naoki
N
Kikuchi
Shinichi
S
Abe
Masafumi
M
D000293:Adolescent; D015153:Blotting, Western; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002868:Chromosomal Proteins, Non-Histone; D018389:Codon, Nonsense; D051546:Cyclooxygenase 2 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D020732:Cytogenetic Analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D066246:ErbB Receptors / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018335:Rhabdoid Tumor / Q000201:enzymology* / Q000473:pathology; D000071796:SMARCB1 Protein; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics
D002868:Chromosomal Proteins, Non-Histone; D018389:Codon, Nonsense; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D000071796:SMARCB1 Protein; C513266:SMARCB1 protein, human; D014157:Transcription Factors; D051546:Cyclooxygenase 2; D066246:ErbB Receptors