title: Smooth endoplasmic reticulum dilation and degeneration in Purkinje neuron dendrites of aging ethanol-fed female rats.
abstract: The effects of chronic ethanol consumption on the extensive Purkinje neuron (PN) dendritic arbor of male rats include dilation of the smooth endoplasmic reticulum (SER) and dendritic regression. The purpose of the present study was to examine the molecular layer of female rats for the presence of ethanol-related SER dilation and evidence of degeneration within the PN dendritic arbor. Twenty-one 12-month-old Fischer 344 female rats (n = 7/treatment group) received a liquid ethanol, liquid control, or rat chow diet for a period of 40 weeks. Ethanol-fed rats received 35% of their dietary calories as ethanol. Pair-fed rats received a liquid control diet that was isocaloric to the ethanol diet. Chow-fed rats received standard laboratory rat chow ad libitum. At the end of treatment, tissues from the anterior and posterior lobes of the cerebellar vermis were viewed and photographed with the electron microscope. The diameters of SER profiles were measured and the density of degenerating bodies within the PN dendritic arbor was quantitated. In the posterior lobe, ethanol-related SER dilation was apparent. In the anterior lobe, the density of degenerating bodies within PN dendritic shafts was significantly increased but SER dilation in PN dendritic shafts was absent. These results confirm that SER dilation and dendritic degeneration in PN dendrites may precede and contribute to ethanol-related regression in female rats. In addition, comparison of these results with data obtained in male rats from a previous study suggests that PN dendrites in females may be more sensitive to the effects of ethanol.
authors.affiliation: Department of Pathology and Anatomical Sciences, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York 14214-3000, USA. cadlugos@buffalo.edu
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Dlugos
mesh_terms: D000375:Aging / Q000502:physiology; D020268:Alcohol-Induced Disorders, Nervous System / Q000473:pathology* / Q000503:physiopathology*; D000818:Animals; D002492:Central Nervous System Depressants / Q000633:toxicity; D003712:Dendrites / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D004195:Disease Models, Animal; D018871:Endoplasmic Reticulum, Smooth / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D046529:Microscopy, Electron, Transmission; D009410:Nerve Degeneration / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D011689:Purkinje Cells / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D051381:Rats; D011916:Rats, Inbred F344; D012727:Sex Characteristics
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol