title: Life-long supplementation with a low dosage of coenzyme Q10 in the rat: effects on antioxidant status and DNA damage.
abstract: Life-long low-dosage supplementation of coenzyme Q(10) (CoQ(10)) is studied in relation to the antioxidant status and DNA damage. Thirty-two male rats were assigned into two experimental groups differing in the supplementation or not with 0.7 mg/kg/day of CoQ(10). Eight rats per group were killed at 6 and 24 months. Plasma retinol, alpha-tocopherol, coenzyme Q, total antioxidant capacity and fatty acids were analysed. DNA strand breaks were studied in peripheral blood lymphocytes. Aging and supplementation led to significantly higher values for CoQ homologues, retinol and alpha-tocopherol. No difference in total antioxidant capacity was detected at 6 months but significantly lower values were found in aged control animals. Similar DNA strand breaks levels were found at 6 months. Aging led to significantly higher DNA strand breaks levels in both groups but animals supplemented with CoQ(10) led to a significantly lower increase in that marker. Aged rats showed significantly higher polyunsaturated fatty acids. This study demonstrates that lifelong intake of a low dosage of CoQ(10) enhances plasma levels of CoQ(9), CoQ(10), alpha-tocopherol and retinol. In addition, CoQ(10) supplementation attenuates the age-related fall in total antioxidant capacity of plasma and the increase in DNA damage in peripheral blood lymphocytes.
authors.affiliation: Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, Spain. jlquiles@ugr.es
authors.forename: José L
authors.initials: JL
authors.lastname: Quiles
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Ochoa
authors.forename: Maurizio
authors.initials: M
authors.lastname: Battino
authors.forename: Purificación
authors.initials: P
authors.lastname: Gutierrez-Rios
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Nepomuceno
authors.forename: Magdalena López
authors.initials: ML
authors.lastname: Frías
authors.forename: Jesús R
authors.initials: JR
authors.lastname: Huertas
authors.forename: José
authors.initials: J
authors.lastname: Mataix
mesh_terms: D000375:Aging / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003067:Coenzymes; D004249:DNA Damage / Q000187:drug effects*; D005227:Fatty Acids / Q000097:blood; D005229:Fatty Acids, Monounsaturated / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood; D014801:Vitamin A / Q000097:blood; D024502:alpha-Tocopherol / Q000097:blood
chemical_list: D000975:Antioxidants; D003067:Coenzymes; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D014801:Vitamin A; D014451:Ubiquinone; C024989:coenzyme Q10; D024502:alpha-Tocopherol; C030571:ubiquinone 9