Analysis of 18F-labelled synthesis products on TLC plates: comparison of radioactivity scanning, film autoradiography, and a phosphoimaging technique.
We compared radioactivity scanning, film autoradiography, and digital photostimulated luminescence (PSL) autoradiography (phosphoimaging technique) in detection of radioactivity on thin-layer chromatography (TLC) plates. TLC combined with radioactivity detection is rapid, simple, and relatively flexible. Here, (18)F-labelled synthesis products were analyzed by TLC and the radioactivity distribution on the plates determined using the three techniques. Radioactivity scanning is appropriate only with good chromatographic resolution and previously validated scanning parameters. Film autoradiography exhibits poor linearity if radioactivity varies greatly. PSL provides high sensitivity and resolution and superior linearity compared with the other methods.
Kämäräinen
Eeva-Liisa
EL
Division of Nuclear Medicine, Helsinki University Central Hospital, P.O. Box 340, FIN-00029 Helsinki, Finland. Eeva-Liisa.Kamarainen@hus.fi
Haaparanta
Merja
M
Siitari-Kauppi
Marja
M
Koivula
Teija
T
Lipponen
Tiina
T
Solin
Olof
O
D000631:Aminopyridines / Q000138:chemical synthesis*; D001345:Autoradiography / Q000379:methods*; D001549:Benzamides / Q000032:analysis* / Q000138:chemical synthesis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D005462:Fluorine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D049449:Luminescence; D010777:Photochemistry; D010879:Piperazines / Q000032:analysis* / Q000138:chemical synthesis*; D012680:Sensitivity and Specificity
D000631:Aminopyridines; D001549:Benzamides; D005462:Fluorine Radioisotopes; D010879:Piperazines; C513891:fluoro-N-(2-(1-(2-methoxyphenyl)-1-piperazinyl)ethyl-N-2-pyridinyl-benzamide); C099463:4-(2' methoxyphenyl)-1-(2'-(N-(2''-pyridinyl)-4-fluorobenzamido)ethyl)piperazine