title: Sequencing and comparative analysis of a pig bovine viral diarrhea virus genome.
abstract: In present study, we report the first complete genomic sequence of pig bovine viral diarrhea (BVD) virus, that of strain ZM-95, which is 12,220 nucleotides long and contains short 5' and 3' non-coding regions and one open reading frame encoding a large polyprotein with 28 potential N-glycosylation sites (Asn-X-Ser or Asn-X-Thr). Within the non-structural protein encoding region, no foreign nucleotide insertions was found as those usually observed for cytopathogenic BVDV-1, but close to the 3'-terminal of the capsid protein (1119-1124bp) it contains a short insertion of a six nucleotide sequence (CTCACA). Three hypervariable regions were identified in the polyprotein-encoding region, with one of them comprising a sequence motif encoding a unique five amino acid peptide HYKKK in glycoprotein E2 gene. The genomic comparison and phylogenetic analyses showed that ZM-95 should be classified into BVDV-1, but was genetically divergent from other pestiviruses sequenced to date since its highest genetic similarity was only 76.6% (with SD-1), therefore, placed as a novel subgroup of BVDV-1.
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun 130062, China.
authors.forename: Xingran
authors.initials: X
authors.lastname: Xu
authors.forename: Qingchan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xinglong
authors.initials: X
authors.lastname: Yu
authors.forename: Long
authors.initials: L
authors.lastname: Liang
authors.forename: Chang
authors.initials: C
authors.lastname: Xiao
authors.forename: Hua
authors.initials: H
authors.lastname: Xiang
authors.forename: Changchun
authors.initials: C
authors.lastname: Tu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D001908:Diarrhea Viruses, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D006031:Glycosylation; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020815:Polyproteins / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014759:Viral Envelope Proteins / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D036022:Capsid Proteins; D020815:Polyproteins; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C076551:gp53, bovine viral diarrhea virus