A method for high-throughput gene expression signature analysis.
Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solution based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.
Peck
David
D
Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA. dpeck@broad.mit.edu
Crawford
Emily D
ED
Ross
Kenneth N
KN
Stegmaier
Kimberly
K
Golub
Todd R
TR
Lamb
Justin
J
D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D018922:HL-60 Cells; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D015203:Reproducibility of Results; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger