[Histopathological examination and analysis of mortality in DBA/2 mouse vasculitis induced with CAWS, a water-soluble extracellular polysaccharide fraction obtained from Candida albicans].
CAWS, a water-soluble extracellular polysaccharide fraction obtained from the culture supernatant of Candida albicans, is one of the fungal pathogen-associated molecular patterns (PAMPs). It has been reported to show potent activity inducing arteritis and coronaritis in mice. Especially, CAWS-induced arteritis has a 100% incidence and severe mortality in the DBA/2 mouse strain. This artificial vasculitis was reported to provide a good murine model of Kawasaki disease and other inflammatory vascular disease. However, severe mortality was observed only in DBA/2 mice, which is a CAWS-sensitive strain. In this study, to clarify the mechanisms of CAWS-induced arteritis and mortality, we investigated microscopic histopathological changes in cardiovascular tissues in DBA/2 mice. Severe inflammatory infiltration was observed from the external elastic lamina in the aorta and proximal coronary arteries within 1 week after CAWS administration. Severe stenosis of the aorta and coronary arteries was observed more than 3 weeks after CAWS administration. Fibrinoid necrosis was observed in these vessel walls. All CAWS-treated mice died between the fifth and twelfth week after administration. Severe inflammatory change with aortic valve transformation suggested that CAWS-treated mice died of valvular endocarditis or cardiac dysfunction. Based on the simple induction method and complete incidence, these data suggest that CAWS-induced arteritis is a good model of not only Kawasaki disease but also other cardiovascular diseases such as valvular endocarditis.
Hirata
Naoto
N
Department of Pharmacy, Nagano Red Cross Hospital, Japan. hirata@nagano-med.jrc.or.jp
Ishibashi
Ken-ichi
K
Ohta
Shin
S
Hata
Satoru
S
Shinohara
Hiroyasu
H
Kitamura
Mio
M
Miura
Noriko
N
Ohno
Naohito
N
D000818:Animals; D001167:Arteritis / Q000139:chemically induced* / Q000401:mortality / Q000473:pathology*; D002176:Candida albicans / Q000737:chemistry / Q000166:cytology / Q000472:pathogenicity*; D002319:Cardiovascular System / Q000473:pathology*; D002458:Cell Fractionation; D004195:Disease Models, Animal; D051379:Mice; D008811:Mice, Inbred DBA; D009080:Mucocutaneous Lymph Node Syndrome; D011134:Polysaccharides / Q000302:isolation & purification / Q000633:toxicity*; D012995:Solubility; D014867:Water
D011134:Polysaccharides; D014867:Water