Identification of outer membrane proteins of Yersinia pestis through biotinylation.
The outer membrane of Gram-negative bacteria contains proteins that might be good targets for vaccines, antimicrobials or detection systems. The identification of surface located proteins using traditional methods is often difficult. Yersinia pestis, the causative agent of plague, was labelled with biotin. Tagged proteins were visualised through streptavidin probing of Western blots. Seven biotinylated proteins of Y. pestis were identified including two porins and the putative virulence factor catalase peroxidase.
Smither
Sophie J
SJ
Dstl, Porton Down, Room 201/Bldg 7a, Salisbury, WILTS, SP4 OJQ, UK. sjsmither@dstl.gov.uk
Hill
Jim
J
van Baar
Ben L M
BL
Hulst
Albert G
AG
de Jong
Ad L
AL
Titball
Richard W
RW
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D019921:Biotinylation; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D010930:Plague / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015010:Yersinia pestis / Q000737:chemistry*
D001425:Bacterial Outer Membrane Proteins