title: SNAP-25 in hippocampal CA3 region is required for long-term memory formation.
abstract: SNAP-25 is a synaptosomal protein of 25 kDa, a key component of synaptic vesicle-docking/fusion machinery, and plays a critical role in exocytosis and neurotransmitter release. We previously reported that SNAP-25 in the hippocampal CA1 region is involved in consolidation of contextual fear memory and water-maze spatial memory (Hou et al. European J Neuroscience, 20: 1593-1603, 2004). SNAP-25 is expressed not only in the CA1 region, but also in the CA3 region, and the SNAP-25 mRNA level in the CA3 region is higher than in the CA1 region. Here, we provide evidence that SNAP-25 in the CA3 region is also involved in learning/memory. Intra-CA3 infusion of SNAP-25 antisense oligonucleotide impaired both long-term contextual fear memory and water-maze spatial memory, with short-term memory intact. Furthermore, the SNAP-25 antisense oligonucleotide suppressed the long-term potentiation (LTP) of field excitatory post-synaptic potential (fEPSP) in the mossy-fiber pathway (DG-CA3 pathway), with no effect on paired-pulse facilitation of the fEPSP. These results are consistent with the notion that SNAP-25 in the hippocampal CA3 region is required for long-term memory formation.
authors.affiliation: Laboratory of Higher Brain Functions, Institute of Neurobiology, Institutes of Brain Science, Fudan University, 220 Han-Dan Road, Shanghai 200433, China.
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Hou
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Qi
authors.initials: Q
authors.lastname: Lu
authors.forename: Xue-Han
authors.initials: XH
authors.lastname: Zhang
authors.forename: Yan-Yang
authors.initials: YY
authors.lastname: Tu
authors.forename: Mei-Lei
authors.initials: ML
authors.lastname: Jin
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Nai-He
authors.initials: NH
authors.lastname: Jing
authors.forename: Bao-Ming
authors.initials: BM
authors.lastname: Li
mesh_terms: D000818:Animals; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology; D005239:Fear / Q000502:physiology; D006624:Hippocampus / Q000033:anatomy & histology* / Q000187:drug effects; D017774:Long-Term Potentiation / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D008568:Memory / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013037:Spatial Behavior / Q000187:drug effects / Q000528:radiation effects; D050825:Synaptosomal-Associated Protein 25 / Q000502:physiology*
chemical_list: D016376:Oligonucleotides, Antisense; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25