Differential molecular identification of Taeniid spp. and Sarcocystis spp. cysts isolated from infected pigs and cattle.
In the present work, the species-specific identification of Taeniid spp. cysticerci and sarcocystis cysts isolated from infected pigs and cattle was achieved by PCR. In particular: (i) multiplex-PCR derived from HDP2 DNA fragment, specific for Taenia saginata/Taenia solium; (ii) PCRs and PCR-RFLPs of the rDNA internal transcribed spacers 1 and 2 (ITS1 and ITS2) for the differential diagnosis of taeniids; (iii) PCR derived from the 18S rRNA gene and sequencing, specific for Sarcoystis spp. The combined application of these three PCR protocols provided an unequivocally specific diagnosis of T. saginata, T. solium, T. hydatigena, Sarcocystis hominis and Sarcocystis suihominis, and may have practical application in the identification of calcified degenerating or morphologically dubious cysts, for example in the slaughter house situation or in human biopsy samples.
González
L M
LM
Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.
Villalobos
N
N
Montero
E
E
Morales
J
J
Sanz
R Alamo
RA
Muro
A
A
Harrison
L J S
LJ
Parkhouse
R M E
RM
Gárate
T
T
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000469:parasitology; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D003937:Diagnosis, Differential; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D012522:Sarcocystis / Q000302:isolation & purification; D012523:Sarcocystosis / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000469:parasitology; D041202:Taenia saginata / Q000302:isolation & purification*; D041201:Taenia solium / Q000302:isolation & purification*; D013622:Taeniasis / Q000175:diagnosis / Q000662:veterinary*
D018509:DNA, Helminth