[The study on the extraction and the antivirus activity of amylose extracted from Grateloupia filicina].
OBJECTIVE
To determine the activity of anti-Herpes simplex II virus (HSV-2) of amylose extracted from Grateloupia filicina.


METHODS
Grateloupia filicina amylose was extracted by five kinds of abstraction methods and its suppression on Herpes simplex II virus was observed on cell level in three aspects: the drug activity of protecting cell, the drug influence of HSV-2 proliferation and the drug synthesis action of HSV-2.


RESULTS
Grateloupia filicina had prominence anti-Herpes simplex II virus activity, IC50 of amylose extracted by water distilling and ethanol sedimentation was 5.80 microg/ml.


CONCLUSIONS
It suggest that the antivirus activity happen in the stage of HSV-2 binding, adsorption and ingression with Vero cell.
Zhu
Yan-Mei
YM
Jinan University, Guangzhou 510632, China.
Wang
Yi-Fei
YF
Zhang
Mei-Ying
MY
Zhu
Liang
L
Kang
Yan-Yan
YY
Men
Xiao-Yuan
XY
Chen
Ying-Zhou
YZ
D000688:Amylose / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D000431:Ethanol; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry; D000461:Rhodophyta / Q000737:chemistry*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects; D014867:Water
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D014867:Water; D000431:Ethanol; D000688:Amylose