Electrostatic contribution to the binding stability of protein-protein complexes.
To investigate roles of electrostatic interactions in protein binding stability, electrostatic calculations were carried out on a set of 64 mutations over six protein-protein complexes. These mutations alter polar interactions across the interface and were selected for putative dominance of electrostatic contributions to the binding stability. Three protocols of implementing the Poisson-Boltzmann model were tested. In vdW4 the dielectric boundary between the protein low dielectric and the solvent high dielectric is defined as the protein van der Waals surface and the protein dielectric constant is set to 4. In SE4 and SE20, the dielectric boundary is defined as the surface of the protein interior inaccessible to a 1.4-A solvent probe, and the protein dielectric constant is set to 4 and 20, respectively. In line with earlier studies on the barnase-barstar complex, the vdW4 results on the large set of mutations showed the closest agreement with experimental data. The agreement between vdW4 and experiment supports the contention of dominant electrostatic contributions for the mutations, but their differences also suggest van der Waals and hydrophobic contributions. The results presented here will serve as a guide for future refinement in electrostatic calculation and inclusion of nonelectrostatic effects.
Dong
Feng
F
Department of Physics, Drexel University, Philadelphia, Pennsylvania, USA.
Zhou
Huan-Xiang
HX
D000110:Acetylcholinesterase / Q000737:chemistry / Q000235:genetics; D018801:CD2 Antigens / Q000737:chemistry / Q000235:genetics; D018968:CD58 Antigens / Q000737:chemistry / Q000235:genetics; D003087:Colicins / Q000737:chemistry / Q000235:genetics; D004546:Elapid Venoms / Q000737:chemistry / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics; D015847:Interleukin-4 / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D009994:Osmolar Concentration; D017354:Point Mutation; D011485:Protein Binding; D019908:Proto-Oncogene Proteins c-raf / Q000737:chemistry / Q000235:genetics; D019948:Receptors, Interleukin-4 / Q000737:chemistry / Q000235:genetics; D012997:Solvents; D055672:Static Electricity; D016559:Tacrolimus / Q000737:chemistry; D022021:Tacrolimus Binding Proteins / Q000737:chemistry / Q000235:genetics; D013816:Thermodynamics; D020827:rap1 GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics
D018801:CD2 Antigens; D018968:CD58 Antigens; D003087:Colicins; D004546:Elapid Venoms; D029968:Escherichia coli Proteins; D019948:Receptors, Interleukin-4; D012997:Solvents; C488859:immE9 protein, E coli; D015847:Interleukin-4; C044937:fasciculin; D019908:Proto-Oncogene Proteins c-raf; D000110:Acetylcholinesterase; D020827:rap1 GTP-Binding Proteins; D022021:Tacrolimus Binding Proteins; D016559:Tacrolimus