Hydrogen peroxide induced apoptosis in SV-40 transformed human salivary gland acinar cells.
We aim to determine whether oxidants induced apoptosis in SV-40 transformed human acinar cells (NS-SV-AC) and to investigate the effects of mitochondria signaling on this process. Morphologic changes of NS-SV-AC after 24h treatment of H(2)O(2) were determined by fluorescence microscope. The expression of caspase 3, poly(ADP-ribose) polymerase (PARP) and cytochrome c released from mitochondria were measured by western blotting. Hydrogen peroxide elicited typical apoptotic morphologic changes (chromatic condensation, nucleus fragmentation). Cytochrome c was significantly augmented in the cytoplasm, caspase 3 and PARP were cleaved by H(2)O(2) treatment. Hydrogen peroxide might induce NS-SV-AC apoptosis in a low concentration and the mitochondria-mediated signal transduction pathway is associated with this process.
Ben-juan
Wei
W
Department of Oral Medicine, 9th People's Hospital, School of Stomatology, Shanghai Jiaotong University, Shanghai 200011, China. benjuanwei@yahoo.com.cn
Zeng-tong
Zhou
Z
D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D045304:Cytochromes c / Q000032:analysis; D003600:Cytosol / Q000378:metabolism; D004789:Enzyme Activation / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D012469:Salivary Glands / Q000166:cytology*; D015398:Signal Transduction / Q000502:physiology
D045304:Cytochromes c; D006861:Hydrogen Peroxide; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3