Fine deletion mapping of chromosome 2q21-37 shows three preferentially deleted regions in oral cancer.
We analysed the loss of heterozygosity (LOH) of long arm of chromosome 2 by using 16 polymorphic microsatellite markers in 39 matched oral normal and cancer tissues, and defined the deletional mapping of the region with putative tumor suppressor genes. LOH was detected at least one location in 33 of 39 (85%) tumor tissues. Frequent deletions were detected at the locations of microsatellite markers, D2S2304 (35%), D2S111 (40%), D2S155 (35%), D2S1327 (29%), D2S164 (29%), D2S125 (68%) and D2S140 (32%). Three preferentially deleted regions at 2q21-24, 2q33-35 and 2q37.3 were observed. Several candidate tumor suppressor genes in these regions such as LRP1B, CASP8, CASP10, BARD1, ILKAP, PPP1R7, and ING5, are located. Further molecular analysis of each gene should be performed to clarify their roles in oral carcinogenesis.
Cengiz
Beyhan
B
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayamashi 700-8525, Japan.
Gunduz
Mehmet
M
Nagatsuka
Hitoshi
H
Beder
Levent
L
Gunduz
Esra
E
Tamamura
Ryo
R
Mahmut
Naila
N
Fukushima
Kunihiro
K
Ali
Mahmoud Al Sheikh
MA
Naomoto
Yoshio
Y
Shimizu
Kenji
K
Nagai
Noriyuki
N
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D009062:Mouth Neoplasms / Q000235:genetics*; D009363:Neoplasm Proteins / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D010749:Phosphoprotein Phosphatases