Mouse oocytes derived from fetal germ cells are competent to support the development of embryos by in vitro fertilization.
Mouse oocyte development in vitro has been studied in the past several years, but no evidence showed that the fertilizable oocytes could be obtained from the fetal mouse germ cells before the formation of the primordial follicles. In this study, an efficient and simple method has been established to obtain the mature oocytes from the fetal mouse germ cells at 16.5 days post-coitum (dpc). For the initial of follicular formation, fetal mouse 16.5 dpc ovaries were transplanted to the recipient under the kidney capsule, and the ovaries were recovered after 14 days. Subsequently, the growing preantral follicles in the ovarian grafts were isolated and cultured in vitro for 12 days. Practically, the mature oocytes ovulated from the antral follicles were able to be fertilized in vitro and support the embryonic development. The results demonstrate that the fetal mouse 16.5 dpc germ cells are able to form primordial follicles with the ovarian pregranulosa cells during the period of transplantation in the ectopic site, and the oocytes within the growing follicles are able to mature in vitro, then are able to support the embryonic development.
Shen
Wei
W
College of Life Sciences, Peking University, Beijing, China.
Li
Lan
L
Zhang
Donghui
D
Pan
Qingjie
Q
Ding
Mingxiao
M
Deng
Hongkui
H
D000818:Animals; D002454:Cell Differentiation; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D005333:Fetus / Q000166:cytology; D005854:Germ Cells / Q000166:cytology / Q000502:physiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D006080:Ovarian Follicle / Q000254:growth & development / Q000637:transplantation; D010053:Ovary / Q000166:cytology / Q000637:transplantation