title: A comparative histologic analysis of tissue-engineered bone using platelet-rich plasma and platelet-enriched fibrin glue.
abstract: OBJECTIVE
  The aim of this study was to compare the effects of platelet-rich plasma (PRP) and platelet-enriched fibrin glue on bone formation in bone tissue engineering.
  
  
  METHODS
  PRP was mixed with bone marrow mesenchymal stem cells and bone morphogenetic protein-2 (BMP-2), and the composites were injected into the subcutaneous space on the dorsum of nude mice. On the contralateral side of the dorsum, platelet-enriched fibrin glue/bone marrow mesenchymal stem cells/BMP-2 composites were injected. Bone formation was evaluated after 12 weeks.
  
  
  RESULTS
  The volumes of subcutaneous nodules formed in nude mice were 55 +/- 18 microL at the PRP/bone marrow mesenchymal stem cells/BMP-2 sites and 135 +/- 27 microL at the platelet-enriched fibrin glue/bone marrow mesenchymal stem cells/BMP-2 sites. Histomorphometric analysis demonstrated that the nodules contained 14.9 +/- 4.1% newly formed bone when using PRP and 19.8 +/- 3.6% newly formed bone when using platelet-enriched fibrin glue.
  
  
  CONCLUSIONS
  The results indicated that the osteogenic characteristics of platelet-enriched fibrin glue are superior to PRP in bone tissue engineering.
authors.affiliation: Department of Dentistry, Yonsei University Wonju College of Medicine, Wonju, South Korea.
authors.forename: Shi-Jiang
authors.initials: SJ
authors.lastname: Zhu
authors.forename: Byung-Ho
authors.initials: BH
authors.lastname: Choi
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Jung
authors.forename: Seoung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Huh
authors.forename: Tae-Min
authors.initials: TM
authors.lastname: You
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jingxu
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001792:Blood Platelets; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D011916:Rats, Inbred F344; D018709:Statistics, Nonparametric; D023822:Tissue Engineering / Q000379:methods*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: C525719:Bmp2 protein, mouse; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D015718:Fibrin Tissue Adhesive; D006133:Growth Substances; D016212:Transforming Growth Factor beta