title: Antigen processing of a short viral antigen by proteasomes.
abstract: Mass spectrometry (MS)-based methods coupled to reverse phase chromatography separation are a useful technology to analyze complex peptide pools that are comprised of different peptides with unrelated sequences. In antigen presentation, proteasomes generate a set of short peptides that are closely related and overlapping and in some instances may even have identical retention times and identical masses. In these situations, micro-liquid chromatography-MS/MS focused on each theoretical parent ion followed by manual interpretation optimizes the identification of generated peptides. The results suggest that the degradation of short antigens by the proteasome occurs by sequential cleavage.
authors.affiliation: Unidad de Proteómica, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Madrid, Spain. dlopez@isciii.es
authors.forename: Daniel
authors.initials: D
authors.lastname: López
authors.forename: Olga
authors.initials: O
authors.lastname: Calero
authors.forename: Mercedes
authors.initials: M
authors.lastname: Jiménez
authors.forename: Margarita
authors.initials: M
authors.lastname: García-Calvo
authors.forename: Margarita
authors.initials: M
authors.lastname: Del Val
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000737:chemistry*; D002845:Chromatography / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits
chemical_list: D000956:Antigens, Viral; D010455:Peptides; D046988:Proteasome Endopeptidase Complex