title: Intra-strain polymorphisms are detected but no genomic alteration is found in cloned mice.
abstract: In-gel competitive reassociation (IGCR) is a method for differential subtraction of polymorphic (RFLP) DNA fragments between two DNA samples of interest without probes or specific sequence information. Here, we applied the IGCR procedure to two cloned mice derived from an F1 hybrid of the C57BL/6Cr and DBA/2 strains, in order to investigate the possibility of genomic alteration in the cloned mouse genomes. Each of the five of the genomic alterations we detected between the two cloned mice corresponded to the "intra-strain" polymorphisms in the C57BL/6Cr and DBA/2 mouse strains. Our result suggests that no severe aberration of genome sequences occurs due to somatic cell nuclear transfer.
authors.affiliation: Kazusa DNA Research Institute, 2-6-7, Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan. koshichi@kazusa.or.jp
authors.forename: Koshichi
authors.initials: K
authors.lastname: Gotoh
authors.forename: Kimiko
authors.initials: K
authors.lastname: Inoue
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ogura
authors.forename: Michio
authors.initials: M
authors.lastname: Oishi
mesh_terms: D000818:Animals; D019976:Cloning, Organism; D016678:Genome; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods