Transcriptional profile of Haemophilus influenzae: effects of iron and heme.
Haemophilus influenzae requires either heme or a porphyrin and iron source for growth. Microarray studies of H. influenzae strain Rd KW20 identified 162 iron/heme-regulated genes, representing approximately 10% of the genome, with > or =1.5-fold changes in transcription in response to iron/heme availability in vitro. Eighty genes were preferentially expressed under iron/heme restriction; 82 genes were preferentially expressed under iron/heme-replete conditions.
Whitby
Paul W
PW
Department of Pediatrics, CHO 2308, 940 NE 13th St., Oklahoma City, OK 73104, USA.
Vanwagoner
Timothy M
TM
Seale
Thomas W
TW
Morton
Daniel J
DJ
Stull
Terrence L
TL
D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006193:Haemophilus influenzae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D006418:Heme / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D046228:Microarray Analysis; D018085:Regulon / Q000235:genetics
D003470:Culture Media; D006418:Heme; D007501:Iron