title: [The application of PCR-SSCP in forensic mtDNA typing].
abstract: OBJECTIVE
  To study the application of PCR-SSCP in forensic mtDNA typing.
  
  
  METHODS
  Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed.
  
  
  RESULTS
  In 70 family trios, the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in 98.57% and 97.13% respectively but were identical with their mothers. In 140 unrelated Wuhan Han individuals, 21 haplotypes were found in HVI, GD = 0.9556; 16 haplotypes were found in HVII, GD = 0.9356.
  
  
  CONCLUSIONS
  PCR-SSCP technique may be useful in forensic mtDNA typing, especially for screening the suspects.
authors.affiliation: Department of Forensic Medicine, Huazhong University of Science and Technology, Wuhan 430030, China. aimingxu03@163.com
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Ai
authors.forename: Chun-Ying
authors.initials: CY
authors.lastname: Yu
authors.forename: Gui-Sen
authors.initials: GS
authors.lastname: Zhao
authors.forename: Dai-Xin
authors.initials: DX
authors.lastname: Huang
authors.forename: Ji
authors.initials: J
authors.lastname: Ke
authors.forename: Xia
authors.initials: X
authors.lastname: Yue
authors.forename: Qing-En
authors.initials: QE
authors.lastname: Yang
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000097:blood / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods; D006239:Haplotypes; D006801:Humans; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial