Neuroproteomics: relevance to anxiety disorders.
Despite advances in the treatment of anxiety disorders, there is a need for medications with greater efficacy and fewer side effects. Advances in techniques to facilitate high throughput, mass analysis of proteins potentially allows for new drug targets, with a shift in focus from membrane receptor proteins and enzymes of neurotransmitter metabolism to molecules in intracellular signal transduction and other pathways. A computerized literature search was done to collect studies on recently developed proteomic techniques and their application in psychiatric research. Particular techniques, such as two-dimensional electrophoresis, two-dimensional differential gel electrophoresis, isotope-coded affinity tags, and isotope tags for relative and absolute quantification, are reviewed. In addition, a combination of these techniques with MALDI-TOF/TOF and ESI-Q-TOF mass spectrometry analysis is discussed in relation to possible novel signaling pathways relevant to anxiety disorders, and to the development of biomarkers for the evaluation of these conditions.
Uys
Joachim D K
JD
MRC Unit for Stress and Anxiety Disorders, Laboratory for Neuroproteomics, Department of Biomedical Sciences, Division of Medical Physiology, University of Stellenbosch, Tygerberg, South Africa. jduys@sun.ac.za
Stein
Dan J
DJ
Daniels
Willie M U
WM
D000818:Animals; D001008:Anxiety Disorders / Q000175:diagnosis* / Q000503:physiopathology*; D001921:Brain / Q000503:physiopathology*; D004195:Disease Models, Animal; D004586:Electrophoresis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D001523:Mental Disorders / Q000175:diagnosis* / Q000503:physiopathology*; D040901:Proteomics / Q000379:methods*