Quantum dot-based fluorescence resonance energy transfer with improved FRET efficiency in capillary flows.
Fluorescence resonance energy transfer (FRET)-based nanosensors with quantum dots (QDs) as donors and organic dyes as acceptors have long been of interest for the detection of biomolecules such as nucleic acids, but their low FRET efficiency in bulk solution has prevented the sensitive detection of nucleic acids due to the large size of the QDs and the long length of nucleic acids. Here we describe a novel approach to improve the detection sensitivity of QD-based nanosensors using single-molecule detection in a capillary flow. In comparison with bulk measurement, single-molecule detection in a capillary flow possesses the unique advantages of improved FRET efficiency, high sensitivity, prevention of photobleaching, and low sample consumption. Greater FRET efficiency was obtained due to the deformation of DNA in the capillary stream. This technique can be easily extended to sensitive bimolecular analysis in microfluidic chips, and it may also offer a promising approach to study the deformation of small nucleic acids in fluid flow.
Zhang
Chun-Yang
CY
Department of Chemistry, York College and The Graduate Center, The City University of New York, Jamaica, New York 11451, USA.
Johnson
Lawrence W
LW
D004247:DNA / Q000032:analysis; D004566:Electrodes; D031541:Fluorescence Resonance Energy Transfer; D044085:Microfluidics; D009841:Oligonucleotides / Q000032:analysis; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D013997:Time Factors
D009841:Oligonucleotides; D004247:DNA