title: Genetic markers of fibrinolytic responses of older persons to exercise training.
abstract: We assessed the interactive effect of genetic polymorphisms and exercise training on fibrinolysis in 50 - 75 yr old men (n = 17) and women (n = 28). Subjects had tissue plasminogen activator (t-PA) antigen levels and activity and plasminogen activator inhibitor-1 (PAI-1) activity measured before and after 6 mo of endurance-exercise training. Subject's DNA was typed for the PAI-1 4 G/5 G and t-PA I/D variants. Baseline PAI-1 activity, t-PA activity, and t-PA antigen levels were not different among PAI-1 or t-PA genotype groups. Overall, exercise training did not change PAI-1 activity (- 0.43 +/- 0.81 IU/mL, p = NS), increased t-PA activity (0.37 +/- 0.16 IU/mL, p = 0.02), and decreased t-PA antigen levels (- 0.88 +/- 0.20 ng/mL, p < 0.001). Although the differences in changes with training were not significant among genotype groups, significant t-PA antigen level improvements were evident only in PAI-1 4 G allele carriers and significant t-PA activity increases only in PAI-1 4 G homozygotes. t-PA genotype affected the training-induced t-PA antigen level improvements (p = 0.033) after covarying for gender and baseline t-PA antigen levels, with the smallest and largest reductions in the D homozygotes and I/D heterozygotes, respectively. These findings could have important treatment implications for the use of exercise training to reduce CV disease and thrombotic risk in older men and women.
authors.affiliation: Department of Kinesiology, University of Maryland, College Park, 20742, USA.
authors.forename: O
authors.initials: O
authors.lastname: Kulaputana
authors.forename: I
authors.initials: I
authors.lastname: Ghiu
authors.forename: D A
authors.initials: DA
authors.lastname: Phares
authors.forename: R E
authors.initials: RE
authors.lastname: Ferrell
authors.forename: R F
authors.initials: RF
authors.lastname: Macko
authors.forename: A P
authors.initials: AP
authors.lastname: Goldberg
authors.forename: J M
authors.initials: JM
authors.lastname: Hagberg
mesh_terms: D000368:Aged; D000941:Antigens / Q000097:blood; D015444:Exercise / Q000502:physiology*; D005260:Female; D005342:Fibrinolysis / Q000235:genetics* / Q000502:physiology; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood / Q000235:genetics*; D010959:Tissue Plasminogen Activator / Q000235:genetics* / Q000276:immunology
chemical_list: D000941:Antigens; D005819:Genetic Markers; D017395:Plasminogen Activator Inhibitor 1; D010959:Tissue Plasminogen Activator