Adhesion properties and competitive pathogen exclusion ability of bifidobacteria with acquired acid resistance.
The adhesion properties of Bifidobacterium longum and Bifidobacterium catenulatum strains with an acquired resistance to acid and their ability to competitively exclude Salmonella enterica serovar Typhimurium, Escherichia coli, Listeria monocytogenes, Enterobacter sakazakii, and Clostridium difficile from adhering to human intestinal mucus were evaluated and compared with the results when the same experiments were run with the original acid-sensitive strains. In half of the four studied cases, the acid-resistant derivative showed a greater ability to adhere to human intestinal mucus than the original strain. The ability of bifidobacteria to inhibit pathogen adhesion to mucus was not generally improved by the acquisition of acid resistance. In contrast, three of the four acid-resistant strains showed a greater ability to displace preadhered pathogens than the original strains, especially preadhered Salmonella Typhimurium and C. difficile. Overall, the induction of acid resistance in bifidobacteria could be a strategy when selecting strains with enhanced stability and improved surface properties that favor their potential functionality as probiotics against specific pathogens.
Collado
M Carmen
MC
Functional Foods Forum, University of Turku, Itäinen Pitkäkatu 4A 5th Floor, FIN 20520, Turku, Finland.
Gueimonde
Miguel
M
Sanz
Yolanda
Y
Salminen
Seppo
S
D000143:Acids / Q000494:pharmacology*; D000222:Adaptation, Physiological; D000898:Antibiosis; D001422:Bacterial Adhesion; D001644:Bifidobacterium / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D024881:Drug Resistance, Bacterial; D005516:Food Microbiology; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D019936:Probiotics
D000143:Acids