title: Role of the RNA2 3' non-translated region of Blackcurrant reversion nepovirus in translational regulation.
abstract: The 3' non-translated regions (NTRs) of mRNAs of eukaryotes and their viruses often contain translational enhancers (TEs). Blackcurrant reversion nepovirus (BRV) has a genome composed of two uncapped polyadenylated RNAs with very long 3' NTRs, nucleotide sequences of which are very conserved between different BRV isolates. In this work, we studied a role of the RNA2 3' NTR in translation, using mutagenesis of the firefly luciferase reporter mRNA, in protoplasts of Nicotiana benthamiana. The RNA2 3' NTR was found to contain a cap-independent TE (3' CITE), which must base pair with the 5' NTR to facilitate translation. The BRV 3' CITE and poly(A) tail provided a major contribution to translational efficiency, with less input from other 3' NTR parts. The BRV 3' CITE does not share similarity in nucleotide sequence and secondary structure with other viruses and thus represents a new class of 3' CITE.
authors.affiliation: Laboratory of Plant Physiology and Molecular Biology, University of Turku, FIN-20014 Turku, Finland. alekar@utu.fi
authors.forename: Alexey
authors.initials: A
authors.lastname: Karetnikov
authors.forename: Mika
authors.initials: M
authors.lastname: Keränen
authors.forename: Kirsi
authors.initials: K
authors.lastname: Lehto
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics / Q000502:physiology*; D020121:5' Untranslated Regions / Q000378:metabolism; D020029:Base Pairing; D001483:Base Sequence; D017930:Genes, Reporter; D049409:Luciferases, Firefly / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D017860:Nepovirus / Q000235:genetics*; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000502:physiology; D012367:RNA, Viral / Q000235:genetics*; D014026:Nicotiana / Q000821:virology
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012333:RNA, Messenger; D012367:RNA, Viral; D049409:Luciferases, Firefly