High frequency of vancomycin-resistant Enterococcus faecium isolates with VanB phenotype and vanA genotype in Korean hospitals.
A total of 59 vancomycin-resistant Enterococcus faecium (VREF) clinical isolates were collected from 8 Korean hospitals for 2 months in 2004. They were investigated by genotyping for glycopeptide resistance, multilocus sequence typing (MLST), esp repeat profiling, and structural analysis of Tn1546-like element. Nine of 59 VREF isolates (15.3%) from 5 hospitals in Korea showed VanB phenotype, but they contained vanA gene. MLST and esp repeat profiling indicated that E. faecium isolates with VanB phenotype and vanA genotype occurred from independent genetic background except 3 isolates from 1 hospital. Structural analysis of Tn1546 also showed that these isolates were not clonally related. Data showed a relatively high frequency of VREF isolates with incongruence between phenotype and genotype for glycopeptide resistance in Korean hospitals.
Song
Jae-Hoon
JH
Asian-Pacific Research Foundation for Infectious Diseases (CARFID), Seoul 135-710, South Korea. jhsong@smc.samsung.co.kr
Ko
Kwan Soo
KS
Oh
Won Sup
WS
Park
Sulhee
S
Heo
Sang Taek
ST
Kwon
Ki Tae
KT
Ryu
Seong Yeol
SY
Peck
Kyong Ran
KR
Lee
Nam Yong
NY
D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D015373:Bacterial Typing Techniques; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D016984:Enterococcus faecium / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006761:Hospitals; D006801:Humans; D007723:Korea; D008565:Membrane Proteins / Q000032:analysis; D008826:Microbial Sensitivity Tests; D040081:Protein Array Analysis; D013045:Species Specificity; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance / Q000235:genetics*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008565:Membrane Proteins; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; C116707:enterococcal surface protein, esp; D014640:Vancomycin; D019729:Carbon-Oxygen Ligases