title: Breath condenser coatings affect measurement of biomarkers in exhaled breath condensate.
abstract: Exhaled breath condensate collection is not yet standardised and biomarker measurements are often close to lower detection limits. In the current study, it was hypothesised that adhesive properties of different condenser coatings interfere with measurements of eicosanoids and proteins in breath condensate. In vitro, condensate was derived from a collection model using two test solutions (8-isoprostane and albumin) and five condenser coatings (silicone, glass, aluminium, polypropylene and Teflon). In vivo, condensate was collected using these five coatings and the EcoScreen condenser to measure 8-isoprostane, and three coatings (silicone, glass, EcoScreen) to measure albumin. In vitro, silicone and glass coatings had significantly higher albumin recovery compared with the other coatings. A similar trend was observed for 8-isoprostane recovery. In vivo, median (interquartile range) 8-isoprostane concentrations were significantly higher using silicone (9.2 (18.8) pg.mL(-1)) or glass (3.0 (4.5) pg.mL(-1)) coating, compared with aluminium (0.5 (2.4) pg.mL(-1)), polypropylene (0.5 (0.5) pg.mL(-1)), Teflon (0.5 (0.0) pg.mL(-1)), and EcoScreen (0.5 (2.0) pg.mL(-1)). Albumin in vivo was mainly detectable using glass coating. In conclusion, a condenser with silicone or glass coating is more efficient for measurement of 8-isoprostane or albumin in exhaled breath condensate, than EcoScreen, aluminium, polypropylene or Teflon. Guidelines for exhaled breath condensate standardisation should include the most valid condenser coating to measure a specific biomarker.
authors.affiliation: Dept of Paediatric Pulmonology, University Hospital Maastricht, Maastricht, The Netherlands. p.rosias@orbisconcern.nl
authors.forename: P P
authors.initials: PP
authors.lastname: Rosias
authors.forename: C M
authors.initials: CM
authors.lastname: Robroeks
authors.forename: H J
authors.initials: HJ
authors.lastname: Niemarkt
authors.forename: A D
authors.initials: AD
authors.lastname: Kester
authors.forename: J H
authors.initials: JH
authors.lastname: Vernooy
authors.forename: J
authors.initials: J
authors.lastname: Suykerbuyk
authors.forename: J
authors.initials: J
authors.lastname: Teunissen
authors.forename: J
authors.initials: J
authors.lastname: Heynens
authors.forename: H J
authors.initials: HJ
authors.lastname: Hendriks
authors.forename: Q
authors.initials: Q
authors.lastname: Jöbsis
authors.forename: E
authors.initials: E
authors.lastname: Dompeling
mesh_terms: D000293:Adolescent; D000328:Adult; D000418:Albumins / Q000032:analysis; D001249:Asthma / Q000276:immunology* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis*; D001944:Breath Tests / Q000295:instrumentation* / Q000379:methods; D002648:Child; D002675:Child, Preschool; D015237:Dinoprost / Q000031:analogs & derivatives / Q000032:analysis; D004867:Equipment Design / Q000295:instrumentation; D005260:Female; D005898:Glass; D006801:Humans; D008297:Male; D008422:Materials Testing; D008875:Middle Aged; D012828:Silicones
chemical_list: D000418:Albumins; D015415:Biomarkers; D012828:Silicones; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost