title: Reaction of porous silicon with both end-functionalized organic compounds bearing alpha-bromo and omega-carboxy groups for immobilization of biomolecules.
abstract: Both end-functionalized (alpha-bromo and omega-carboxy) compounds were first tested for the radical reaction on the silicon-hydride (Si-H) terminated porous silicon (PSi) with/without the presence of diacyl peroxide initiator under microwave irradiation. Then the carboxylic acid monolayers (CAMs) assembled on PSi through the robust Si-C bonds were converted to amino-reactive linker, N-hydroxysuccinimide (NHS)-ester, terminated monolayers. And finally two proteins of bovine serum albumin (BSA) and lysozyme (Lys) were immobilized through amide bonds. The optimum PSi membrane for protein immobilization without collapse, with parameters of porous radii 4-10 nm and depth 0.2-4.6 mum, was prepared from the (100)-oriented p-type silicon wafer. The chemically converted surface products were monitored with Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FESEM).
authors.affiliation: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P.R. China.
authors.forename: Dong-Jie
authors.initials: DJ
authors.lastname: Guo
authors.forename: Shou-Jun
authors.initials: SJ
authors.lastname: Xiao
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.forename: Shuai
authors.initials: S
authors.lastname: Wei
authors.forename: Jia
authors.initials: J
authors.lastname: Pei
authors.forename: Yi
authors.initials: Y
authors.lastname: Pan
authors.forename: Xiao-Zeng
authors.initials: XZ
authors.lastname: You
authors.forename: Zhong-Ze
authors.initials: ZZ
authors.lastname: Gu
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000818:Animals; D001966:Bromine; D002264:Carboxylic Acids / Q000737:chemistry*; D002417:Cattle; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D009930:Organic Chemicals / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012825:Silicon; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D002264:Carboxylic Acids; D009930:Organic Chemicals; D012710:Serum Albumin, Bovine; D001966:Bromine; D012825:Silicon