Determination of atropine in pharmaceutical dosage forms containing vegetal preparations, by high-performance liquid chromatography with U.V. and electrochemical detection.
A method to test the uniformity of Belladonna powder in multicomponent pharmaceutical dosage forms, by the determination of atropine, is described. The procedure involves a liquid-liquid extraction, followed by liquid chromatographic separation of atropine, apoatropine and scopolamine and quantification of atropine with either ultraviolet or electrochemical detection. The method is shown to be selective, sensitive and offers good reproducibility. A detailed study of the electrochemical properties of atropine also is undertaken.
Leroy
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Laboratoire de Chimie Analytique et Bromatologie, Faculté des Sciences Pharmaceutiques et Biologiques, 7, rue Albert Lebrun, B.P. 403, 54001 Nancy Cedex, France.
Nicolas
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A