Quantitative analysis of CCR5 chemokine receptor and cytochrome P450 aromatase transcripts in swim-up spermatozoa isolated from fertile and infertile men.
We determined the CCR5 chemokine receptor and cytochrome P450 aromatase (P450arom) transcript copies number in swim-up sperm isolated from fertile and infertile men. The ejaculates were purified by centrifugation through discontinuous Percoll density gradient and swim-up techniques. RNA was isolated from sperm, treated with DNase I and reverse-transcribed into cDNA. Quantitative analysis of CCR5 and P450arom cDNA were performed by real-time quantitative (RQ-PCR) SYBR Green I analysis. There was a higher content of CCR5 and P450arom transcripts copy number in swim-up sperm of fertile than from infertile donors. The decrease in CCR5 and P450arom transcripts in swim-up sperm may be associated with male infertility.
Jedrzejczak
P
P
Department of Infertility and Reproductive Endocrinology, Poznań University of Medical Sciences, Poznań, Poland.
Januchowski
R
R
Taszarek-Hauke
G
G
Laddach
R
R
Pawelczyk
L
L
Jagodzinski
P P
PP
D001141:Aromatase / Q000235:genetics*; D005298:Fertility / Q000235:genetics*; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D019713:Receptors, CCR5 / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism*
D012333:RNA, Messenger; D019713:Receptors, CCR5; D001141:Aromatase