Similar functional activity of dendritic cells recruited to the mesenteric lymph nodes of newborn and adult mice after the rectal delivery of Mycobacterium bovis BCG.
BCG rectal administration to newborn and adult mice induced protective immune responses against tuberculosis. BCG reaches the sub-epithelial site and the draining mesenteric lymph nodes (MLNs), and dendritic cells (DC) could be recruited to these sites. Using polarized Caco-2 epithelial cells, we showed that BCG translocates epithelial cells to basolateral compartment. Delayed in newborn BALB/c mice, an important recruitment of CD11c+ DCs, was documented in the rectal lamina propria and the MLNs during the first two weeks after rectal BCG delivery. In MLNs, two major DC subtypes were observed: conventional DCs (cDCs) (B220-) and plasmacytoid DCs (pDCs) (B220+). CIRE, mouse DC-specific intracellular adhesion molecule 3 grabbing non-integrin (DC-SIGN) is predominantly expressed on pDCs and at a higher level on pDCs from the adult compared to newborn MLNs. cDCs with a higher capacity to induce the proliferation of naïve CD4+ T cells than pDCs, triggered CD4+ T cells to produce interferon-gamma whereas pDCs triggered them to release interleukin-10. Both DC subtypes equilibrates T cells as a source of microbicidal/microbiostatic signals and those acting as source of counter-inflammatory signals, preventing tissue damage and/or accelerating tissue repair. Thus, rectal delivery of BCG could be a safe and efficient route of vaccination against tuberculosis.
Abolhassani
Mohammad
M
Laboratoire de Référence des Mycobactéries, Institut Pasteur, 75015 Paris, France.
Lagranderie
Micheline
M
Caminshi
Irina
I
Romain
Felix
F
Balazuc
Anne-Marie
AM
Wagner
Marie-Christine
MC
Tanguy
Myriam
M
Fiette
Laurence
L
Sobhani
Iradj
I
Milon
Geneviève
G
Marchal
Gilles
G
D000285:Administration, Rectal; D000818:Animals; D000831:Animals, Newborn; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology* / Q000493:pharmacokinetics; D018845:CD11 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018938:Caco-2 Cells; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology*; D019402:Macrophage Inflammatory Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012007:Rectum / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control*
D001500:BCG Vaccine; C108355:CCL20 protein, human; D018845:CD11 Antigens; D054418:Chemokine CCL20; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D007371:Interferon-gamma