title: SIGNATURE: a single-particle selection system for molecular electron microscopy.
abstract: SIGNATURE is a particle selection system for molecular electron microscopy. It applies a hierarchical screening procedure to identify molecular particles in EM micrographs. The user interface of the program provides versatile functions to facilitate image data visualization, particle annotation and particle quality inspection. The system design emphasizes both functionality and usability. This software has been released to the EM community and has been successfully applied to macromolecular structural analyses.
authors.affiliation: Howard Hughes Medical Institute, W. M. Keck Institute for Cellular Visualization, Department of Biochemistry, Brandeis University, Waltham, MA 22454-9110, USA. jzchen@brandeis.edu
authors.forename: James Z
authors.initials: JZ
authors.lastname: Chen
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Grigorieff
mesh_terms: D000465:Algorithms; D019295:Computational Biology; D020285:Cryoelectron Microscopy / Q000379:methods*; D006433:Hemocyanins / Q000737:chemistry; D010363:Pattern Recognition, Automated / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin