Effects of 8-2 fluorotelomer alcohol on oleic acid formation in the liver of rats.
Effects of 8-2 fluorotelomer alcohol on fatty acid composition of lipid in the liver of rats were investigated. Feeding of male rats with a diet that contained 8-2 fluorotelomer alcohol at concentrations of 0.2, 0.4 and 0.8% (w/w) for 14 d caused a significant increase in proportion and content of oleic acid (18 : 1 (n-9)) in the liver. The treatment of rats with 8-2 fluorotelomer alcohol increased activities of palmitoyl-CoA chain elongase (PCE) and stearoyl-CoA desaturase (SCD) and mRNA expressions for rat fatty acid elongase 2 (rELO2) and stearoyl-CoA desaturase 1 (SCD1), but neither rat fatty acid elongase 1 (rELO1) or stearoyl-CoA desaturase 2 (SCD2), in the liver in dose-dependent manners. Multiple regression analyses, which were performed to estimate relative contribution of PCE and SCD for determination of the proportion or the content of 18 : 1 (n-9), revealed that the three parameters were significantly correlated and that standardized partial regression coefficient of PCE was higher than that of SCD. These results suggest that 8-2 fluorotelomer alcohol caused considerable changes in the composition and the content of fatty acid, especially 18 : 1 (n-9), in the liver by inducing PCE and SCD, and that PCE plays a crucial role in the increased proportion of 18 : 1 (n-9) in the liver of the rats given fluorotelomer alcohol.
Iwase
Yuko
Y
Faculty of Pharmaceutical Sciences, Josai University, Japan.
Kudo
Naomi
N
Toyama
Tomoaki
T
Tamura
Masafumi
M
Mitsumoto
Atsushi
A
Kawashima
Yoichi
Y
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005233:Fatty Alcohols / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D019301:Oleic Acid / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013230:Stearoyl-CoA Desaturase / Q000235:genetics; D005466:Fluorocarbons
D017931:DNA Primers; D005233:Fatty Alcohols; D019301:Oleic Acid; D012333:RNA, Messenger; D013230:Stearoyl-CoA Desaturase; C505284:1,1,2,2-tetrahydroheptadecafluorodecanol-3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluoro-1-decanol; D005466:Fluorocarbons