title: Chromosome analysis of uncultured amniocytes by comparative genomic hybridization in early amniocentesis.
abstract: OBJECTIVE
  To study chromosome analysis by comparative genomic hybridization (CGH) compared with the conventional technique in early amniocentesis.
  
  
  METHODS
  Cross-sectional descriptive study design was performed in 32 singleton pregnant women with gestational age between 12-15 weeks. Transabdominal amniocentesis was carried out under ultrasound guidance. The amniotic fluid samples were simultaneously investigated using CGH and the conventional cytogenetics study as a gold standard.
  
  
  RESULTS
  Amniocentesis were done for advanced maternal age in all cases. The mean maternal age was 35.8 years (35-42 years). The mean gestational age was 13.7 weeks (12-15 weeks). The chromosome analysis by CGH technique of uncultured amniocyte showed 17 normal female chromosomes (53.1%) and 15 normal male chromosomes (46.9%). This finding was the same as the conventional cytogenetics method. The mean duration of the CGH method was 6 days and that of the conventional cytogenetics method was 13.7 days (10-23 days).
  
  
  CONCLUSIONS
  The CGH technique is a reliable technique for a rapid prenatal diagnosis of chromosome study in early gestation.
authors.affiliation: Department of Obstetrics, Phramongkutklao Hospital, Bangkok, Thailand. chrodna@yahoo.com
authors.forename: Aphornpirom
authors.initials: A
authors.lastname: Ketupanya
authors.forename: Niwat
authors.initials: N
authors.lastname: Aranyakasemsuk
authors.forename: Chintana
authors.initials: C
authors.lastname: Tocharoentanaphol
authors.forename: Chutawadi
authors.initials: C
authors.lastname: Vuthiwong
mesh_terms: D000328:Adult; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology*; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008297:Male; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D015203:Reproducibility of Results