title: Conventional and novel methods for embryonic stem cell line derivation.
abstract: Cell therapy is the promising therapeutic tool for the next decade. "Regenerative Medicine" based on cell and tissue replacement therapy is proposed as a revolutionary approach to various chronic and incurable conditions. The first key step for successful cell therapy is the establishment of clinical grade human Embryonic Stem Cell (hESC) lines. This article provides a concise summary on conventional and novel methods for hESC line derivation. There is also discussion on progression, future direction and problems in hESC line development. In Thailand, more advance knowledge, skill, and technology are required to develop the first human embryonic stem cell line and step forward to make cell therapy a reality.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. ksorapop@yahoo.com
authors.forename: Sorapop
authors.initials: S
authors.lastname: Kiatpongsan
authors.forename: Yuen
authors.initials: Y
authors.lastname: Tannirandorn
authors.forename: Pranee
authors.initials: P
authors.lastname: Numchaisrikhabsc
authors.forename: Ruttachuk
authors.initials: R
authors.lastname: Rungsiwiwut
mesh_terms: D001757:Blastomeres / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology*; D033581:Stem Cell Transplantation; D013785:Thailand