title: In vitro and in vivo stability of plasmids in attenuated Salmonella enterica serovar typhimurium used as a carrier of DNA vaccine is associated with its replication origin.
abstract: The ability of live attenuated Salmonella enterica serovar typhimurium (S. typhimurium) as a carrier of DNA vaccine was evaluated using model plasmid encoding beta-galactosidase (beta-Gal) and BALB/c mice. We constructed pBRCMVbeta, beta-Gal expression apparatus having a replication origin from low copy pBR322. Comparison of the plasmid stability showed that pBRCMVbeta remained stable in Salmonella even after oral administration, while pUC-based pCMVbeta tended to be lost quickly. However, titers for beta-Gal specific IgG in sera did not significantly increase in mice orally administered S. typhimurium harboring pBRCMVbeta. These data suggest that the stability of plasmid in S. typhimurium is associated with its replication origin. Further studies are required to scientifically establish this methodology.
authors.affiliation: Department of Veterinary Microbiology, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Haga
authors.forename: Shino
authors.initials: S
authors.lastname: Kumabe
authors.forename: Ai
authors.initials: A
authors.lastname: Ikejiri
authors.forename: Yuya
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Huang
authors.initials: H
authors.lastname: Li
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Goto
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsui
authors.forename: Hironori
authors.initials: H
authors.lastname: Miyata
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Miura
mesh_terms: D000284:Administration, Oral; D000818:Animals; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D014677:Pharmaceutical Vehicles; D010957:Plasmids / Q000502:physiology*; D012486:Salmonella typhimurium; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA / Q000008:administration & dosage*; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D014677:Pharmaceutical Vehicles; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D001616:beta-Galactosidase