Production of justicidin B, a cytotoxic arylnaphthalene lignan from genetically transformed root cultures of Linum leonii.
Callus and hairy root cultures of Linum leonii were established. The genetic transformation in hairy roots was proven by PCR analysis, which showed integration of rol A and rol C genes into the plant genome. Calli and hairy roots accumulate the arylnaphthalene lignan justicidin B as a major constituent. Hairy roots produce 5-fold higher yields of justicidin B (10.8 mg g(-1) DW) compared to calli. Justicidin B shows strong cytotoxicity on the chronic myeloid leukemia LAMA-8 and K-562 cell lines and on the chronic lymphoid leukemia SKW-3 cell line with IC(50) values of 1.11, 6.08, and 1.62 microM, respectively. Apoptotic properties of justicidin B are reported for the first time.
Vasilev
Nikolay
N
Department of Pharmacognosy and Botany, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav, Sofia 1000, Bulgaria.
Elfahmi
Bos
Rein
R
Kayser
Oliver
O
Momekov
Georgi
G
Konstantinov
Spiro
S
Ionkova
Iliana
I
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004148:Dioxolanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D019597:Flax / Q000737:chemistry* / Q000235:genetics*; D005602:France; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000235:genetics*
D000972:Antineoplastic Agents, Phytogenic; D004148:Dioxolanes; D017705:Lignans; C049719:justicidins