title: Quantitative assessment of olfactory receptors activity in immobilized nanosomes: a novel concept for bioelectronic nose.
abstract: We describe how mammalian olfactory receptors (ORs) could be used as sensing elements of highly specific and sensitive bioelectronic noses. An OR and an appropriate G(alpha) protein were co-expressed in Saccharomyces cerevisiae cells from which membrane nanosomes were prepared, and immobilized on a sensor chip. By Surface Plasmon Resonance, we were able to quantitatively evaluate OR stimulation by an odorant, and G protein activation. We demonstrate that ORs in nanosomes discriminate between odorant ligands and unrelated odorants, as in whole cells. This assay also provides the possibility for quantitative assessment of the coupling efficiency of the OR with different G(alpha) subunits, without the interference of the cellular transduction pathway. Our findings will be useful to develop a new generation of electronic noses for detection and discrimination of volatile compounds, particularly amenable to micro- and nano-sensor formats.
authors.affiliation: Unité de Neurobiologie de l'Olfaction et de la Prise Alimentaire, Equipe Récepteurs et Communication Chimique, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cedex, France. jasmina.minic@jouy.inra.fr
authors.forename: Jasmina Minic
authors.initials: JM
authors.lastname: Vidic
authors.forename: Jeanne
authors.initials: J
authors.lastname: Grosclaude
authors.forename: Marie-Annick
authors.initials: MA
authors.lastname: Persuy
authors.forename: Josiane
authors.initials: J
authors.lastname: Aioun
authors.forename: Roland
authors.initials: R
authors.lastname: Salesse
authors.forename: Edith
authors.initials: E
authors.lastname: Pajot-Augy
mesh_terms: D000818:Animals; D021522:Bioartificial Organs; D015374:Biosensing Techniques; D018914:Cells, Immobilized; D044385:GTP-Binding Protein alpha Subunits / Q000737:chemistry / Q000235:genetics; D006801:Humans; D051379:Mice; D053758:Nanoparticles; D009812:Odorants / Q000032:analysis*; D051381:Rats; D018035:Receptors, Odorant / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D044385:GTP-Binding Protein alpha Subunits; D018035:Receptors, Odorant; D011994:Recombinant Proteins