title: Measurement of bubble and pellet size distributions: past and current image analysis technology.
abstract: Measurements of bubble and pellet size distributions are useful for biochemical process optimizations. The accuracy, representation, and simplicity of these measurements improve when the measurement is performed on-line and in situ rather than off-line using a sample. Historical and currently available measurement systems for photographic methods are summarized for bubble and pellet (morphology) measurement applications. Applications to cells, mycelia, and pellets measurements have driven key technological developments that have been applied for bubble measurements. Measurement trade-offs exist to maximize accuracy, extend range, and attain reasonable cycle times. Mathematical characterization of distributions using standard statistical techniques is straightforward, facilitating data presentation and analysis. For the specific application of bubble size distributions, selected bioreactor operating parameters and physicochemical conditions alter distributions. Empirical relationships have been established in some cases where sufficient data have been collected. In addition, parameters and conditions with substantial effects on bubble size distributions were identified and their relative effects quantified. This information was used to guide required accuracy and precision targets for bubble size distribution measurements from newly developed novel on-line and in situ bubble measurement devices.
authors.affiliation: Fermentation and Development Operations, Merck Research Laboratories, RY810-127, PO Box 2000, Rahway, NJ 07065, USA. beth_junker@merck.com
authors.forename: Beth
authors.initials: B
authors.lastname: Junker
mesh_terms: D005544:Forecasting; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D045423:Microbubbles; D008853:Microscopy / Q000379:methods* / Q000639:trends*; D049329:Nanostructures / Q000648:ultrastructure*; D010316:Particle Size