[Relationship between expression of nestin in experimental brain contusion and injury time].
OBJECTIVE
To observe the alteration of nestin intervals in the experimental traumatic brain injury and investigate its relation to the injury intervals.


METHODS
The rat brain contusion was conducted by falling impact injury. After various survival interval (0.5, 6, 12 h and 1, 3, 7, 14, 28 d), immunohistochemical SP method was used for observing the expression of nestin in the cortex, hippocampal dentate gyrus and the corpus callosum on injury side.


RESULTS
Expression of nestin positive cells increased at 0.5 h and reached the maximum level in 7 d after brain contusion, then the expression decreased gradually. The intensity of nestin staining in the the cortex and the hippocampal dentate gyrus decreased to normal on 28 d. As to the corpus callosum of injury side it remained weak on 28 d.


CONCLUSIONS
The changes of nestin immunohistochemical staining can be used as an index for forensic estimation of early injury time.
Jia
Dong-Mei
DM
Department of Forensic Pathology, Tongji Medical college, Huazhong University of Science and Technology, Wuhan 430030, China.
He
Guang-Long
GL
Zhou
Yi-Wu
YW
Deng
Wei-Nian
WN
Sun
Lai-Jing
LJ
Dai
Jia-Pei
JP
Liu
Liang
L
Xu
Xiao-Hu
XH
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013997:Time Factors
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin