title: Simultaneous determination of palladium, platinum and rhodium by on-line column enrichment and HPLC with 5-(2,4-dihydroxyphenylazo)-rhodanine as pre-column derivatization reagent.
abstract: In this paper, a new method for the simultaneous determination of palladium, platinum and rhodium ions was developed using a rapid column high performance liquid chromatography equipped with on-line enrichment technique. The palladium, platinum and rhodium ions were pre-column derivatized with DHAR to form colored chelates. The Pb-DHAR, Pt-DHAR and Rh-DHAR chelates could be absorbed onto the front of the enrichment column when they were injected into the injector and sent to the enrichment column [ZORBAX Stable Bound, 4.6 x 10 mm, 1.8 microm] with a 0.05 mol L(-1) of phosphoric acid solution as mobile phase. After enrichment, and by switching the six ports switching valve, the retained chelates were back-flushed by mobile phase and traveling towards the analytical column. The separation of these chelates on the analytical column [ZORBAX Stable Bound, 4.6 x 50 mm, 1.8 microm] was satisfactory with 54% acetonitrile (containing 0.05 mol L(-1) of phosphoric acid and 0.1% of tritonX-100) as mobile phase. Palladium, platinum and rhodium were separated completely within 2 min. By on-line enrichment technique, the enrichment factor of 100 was achieved, and the detection limits (S/N = 3) of palladium, platinum and rhodium reaches 1.4 ng L(-1), 1.6 ng L(-1) and 2.0 ng L(-1), respectively. This method was applied to the determination of palladium, platinum and rhodium in water, urine and soil samples with good results.
authors.affiliation: Department of Chemistry, Yunnan University, Kunming 650091, P R China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Li
authors.forename: Xuemei
authors.initials: X
authors.lastname: Li
authors.forename: Qiufen
authors.initials: Q
authors.lastname: Hu
authors.forename: Jiayuan
authors.initials: J
authors.lastname: Yin
authors.forename: Jing
authors.initials: J
authors.lastname: Chzn
authors.forename: Guangyu
authors.initials: G
authors.lastname: Yang
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002138:Calibration; D002614:Chelating Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D010165:Palladium / Q000032:analysis* / Q000652:urine; D010756:Phosphoric Acids / Q000737:chemistry; D010984:Platinum / Q000032:analysis* / Q000652:urine; D015203:Reproducibility of Results; D012236:Rhodanine / Q000031:analogs & derivatives* / Q000737:chemistry*; D012238:Rhodium / Q000032:analysis* / Q000652:urine; D012680:Sensitivity and Specificity; D012987:Soil / Q000032:analysis; D014867:Water / Q000032:analysis
chemical_list: C514154:5-(2,4-dihydroxyphenylazo)rhodanine; D000097:Acetonitriles; D002614:Chelating Agents; D010756:Phosphoric Acids; D012987:Soil; D014867:Water; D010984:Platinum; D010165:Palladium; D012236:Rhodanine; D012238:Rhodium; C030242:phosphoric acid; C032159:acetonitrile