Replacement of microbiological assay by high-performance liquid chromatographic assay for antibiotics.
The current position with respect to the expression of biological potency and chemical purity of antibiotics is reviewed. In particular, attention is drawn to the policies adopted in the USA which have been a source of confusion regarding the expression of biological potency. It is suggested that the opportunity provided by the introduction of high-performance liquid chromatography for the assay of antibiotics should be used to seek adoption of a more consistent approach for expressing antibiotic purity.
Thomas
A H
AH
Division of Antibiotics and Chemistry, National Institute for Biological Standards and Control, Blanche Lane, Potters Bar, Hertfordshire EN6 3QG, UK.