title: [Inhibition of invasiveness and expression of heparanase-mRNA in human malignant melanoma cell line A375 by matrine].
abstract: OBJECTIVE
  To investigate the effect of matrine on the invasiveness and expression of heparanase-mRNA in human malignant melanoma cell line A375.
  
  
  METHODS
  The A375 cells were treated by matrine in different concentration. The total RNAs were extracted from the cells 48 hours after treatment and then semi-quantitative RT-PCR were performed to evaluate the heparanase-mRNA expression levels. Effect of matrine on adhesion of treated A375 cells was tested by cell-Matrigel adhesion assay. The invasiveness of treated A375 cells was measured by Matrigel invasion assay.
  
  
  RESULTS
  The hepanase-mRNA expression, adhesion and invasiveness of A375 cells treated with matrine of different final concentrations significantly decreased compared with that of the controls (p < 0.01). Besides, the inhibitory effects were signifcantly different when the cells treated with matrine of different concentrations (P < 0.01).
  
  
  CONCLUSIONS
  By down-regulating the expression of heparanase-mRNA, matrine has a significant inhibitory effect on the adhesion and invasiveness of human malignant melanoma cell line in a dose-dependent manner.
authors.affiliation: The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Fang
authors.forename: Zheng-Gang
authors.initials: ZG
authors.lastname: Yang
authors.forename: Li-Song
authors.initials: LS
authors.lastname: Teng
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Ruan
authors.forename: De-Ren
authors.initials: DR
authors.lastname: Fang
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Jiang
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008545:Melanoma / Q000201:enzymology / Q000473:pathology*; D009361:Neoplasm Invasiveness; D010946:Plants, Medicinal / Q000737:chemistry; D011807:Quinolizines / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029909:Sophora / Q000737:chemistry*; D000093842:Matrines; D000099175:Heparanase
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D005966:Glucuronidase; D011807:Quinolizines; D012333:RNA, Messenger; D000093842:Matrines; D000099175:Heparanase