title: [Conservation of Malassezia strains in blotting paper].
abstract: Reference strains belonging to the genus Malassezia were analyzed to evaluate, by comparison, different preservation systems such us subculture, freezing at -80 degrees C in glycerol, and blotting paper-disc conservation. The viability, phenotypic and genotypic characteristics of the strains used in this study was evaluated. The blotting paper method was found to be advantageous to preserve Malassezia spp strains due to both, its simple implementation in the laboratory and its efficiency.
authors.affiliation: Centro de Referencia en Micología, Facultad de Ciencias Bioquímicas y Farmaciéuticas, Suipacaha 531, 2000 Universidad Nacional de Rosario, Rosario, Argentina. ramos.laura@tower.com.ar
authors.forename: Laura
authors.initials: L
authors.lastname: Ramos
authors.forename: Silvana
authors.initials: S
authors.lastname: Ramadán
authors.forename: Clara
authors.initials: C
authors.lastname: López
authors.forename: Lucía
authors.initials: L
authors.lastname: Bulacio
authors.forename: Soledad
authors.initials: S
authors.lastname: Mellado
mesh_terms: D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D005838:Genotype; D005990:Glycerol; D008290:Malassezia / Q000187:drug effects / Q000235:genetics; D009172:Mycology / Q000379:methods* / Q000592:standards; D010209:Paper; D010641:Phenotype; D011309:Preservation, Biological; D012015:Reference Standards
chemical_list: D003451:Cryoprotective Agents; D005990:Glycerol