title: Conjugated linolenic acid is slowly absorbed in rat intestine, but quickly converted to conjugated linoleic acid.
abstract: We showed previously that alpha-eleostearic acid (alpha-ESA; 9Z11E13E-18:3) is converted to 9Z11E-conjugated linoleic acid (CLA) in rats through a Delta13-saturation reaction. To investigate this further, we examined the absorption and metabolism of alpha-ESA in rat intestine using a lipid absorption assay in lymph from the thoracic duct. In this study, we used 4 test oils [tung oil, perilla oil, CLA-triacylglycerol (TG), and pomegranate seed oil, containing alpha-ESA, alpha-linolenic acid (LnA; 9Z12Z15Z-18:3), CLA, and punicic acid (PA; 9Z11E13Z-18:3), respectively]. Emulsions containing the test oils were administered to rats, and lymph from the thoracic duct was collected over 24 h. The positional and geometrical isomerism of CLA produced by PA metabolism was determined using GC-electron impact (EI)-MS and (13)C-NMR, respectively; the product was confirmed to be 9Z11E-CLA. A part of alpha-ESA and PA was converted to 9Z11E-CLA 1 h after administration; therefore the lymphatic recoveries of alpha-ESA and PA were modified by the amount of recovered CLA. Cumulative recovery of CLA, alpha-ESA, and PA was lower than that of LnA only during h 1 (P < 0.05), and cumulative recovery of alpha-ESA and PA was significantly lower than that of LnA and CLA for 8 h (P < 0.05). Therefore, the absorption rate was LnA > CLA > alpha-ESA = PA. The conversion ratio of alpha-ESA to 9Z11E-CLA was higher than that of PA to 9Z11E-CLA over 24 h (P < 0.05). These results indicated that alpha-ESA and PA are slowly absorbed in rat intestine, and a portion of these fatty acids is quickly converted to 9Z11E-CLA.
authors.affiliation: Laboratory of Food and Biomolecular Science, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan. tsuduki@myu.ac.jp
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tsuzuki
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Renpei
authors.initials: R
authors.lastname: Abe
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Kazunori
authors.initials: K
authors.lastname: Koba
authors.forename: Jun
authors.initials: J
authors.lastname: Imamura
authors.forename: Toshio
authors.initials: T
authors.lastname: Iwata
authors.forename: Ikuo
authors.initials: I
authors.lastname: Ikeda
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000378:metabolism / Q000493:pharmacokinetics; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism*; D007536:Isomerism; D044243:Linoleic Acids, Conjugated / Q000378:metabolism* / Q000493:pharmacokinetics; D008297:Male; D010938:Plant Oils / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017962:alpha-Linolenic Acid / Q000378:metabolism* / Q000493:pharmacokinetics
chemical_list: D016588:Anticarcinogenic Agents; D044243:Linoleic Acids, Conjugated; D010938:Plant Oils; D017962:alpha-Linolenic Acid; C058216:perilla seed oil