title: Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae.
abstract: We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.
authors.affiliation: Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume, Fukuoka 839-0861, Japan. ooba@fitc.pref.fukuoka.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Oba
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Shuji
authors.initials: S
authors.lastname: Nomiyama
authors.forename: Hikaru
authors.initials: H
authors.lastname: Suenaga
authors.forename: Shigeru
authors.initials: S
authors.lastname: Muta
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tashiro
authors.forename: Satoru
authors.initials: S
authors.lastname: Kuhara
mesh_terms: D015079:2-Isopropylmalate Synthase / Q000235:genetics / Q000378:metabolism*; D025141:Drug Resistance, Fungal; D007930:Leucine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D000439:Pentanols / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000439:Pentanols; D029701:Saccharomyces cerevisiae Proteins; C035177:5',5',5'-trifluoroleucine; C029683:isopentyl alcohol; D015079:2-Isopropylmalate Synthase; C080062:LEU4 protein, S cerevisiae; D007930:Leucine