Fifteen polymorphisms in endothelin-1, endothelin-2 and endothelin-receptor-A genotyped by four duplex assays and seven simple assays on a LightCycler using hybridization probes.
Endothelins (EDNs) are peptides, produced by various tissues, with potent vasoactive and mitogenic properties. Endothelin actions are mediated via specific G protein-coupled receptors of two subtypes, endothelin-receptor-A (EDNRA) and endothelin-receptor-B (EDNRB). Some polymorphisms of the EDN1, EDN2 and EDNRA genes may influence susceptibility to vascular diseases. Thus, genotyping for polymorphisms of these genes may represent a tool for predicting individual susceptibility to vascular diseases. Here, we report 11 fluorescence resonance energy transfer (FRET) assays for the detection of 15 polymorphisms, because the assays used in previous studies (allele-specific PCR and restriction fragment-length polymorphism assays) for some of these polymorphisms are laborious and time-consuming. The newly developed assays are fast and work without expensive ready-to-use mixtures.
Arjomand-Nahad
Farhad
F
Institute of Clinical Pharmacology, Campus Charité Mitte, Charité-Universitätsmedizin Berlin, Berlin, Germany. farhad.arjomand@gmx.de
Landt
Olfert
O
Stangl
Karl
K
Diefenbach
Konstanze
K
Roots
Ivar
I
D015342:DNA Probes; D019332:Endothelin-1 / Q000235:genetics*; D019333:Endothelin-2 / Q000235:genetics*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005838:Genotype; D006801:Humans; D009693:Nucleic Acid Hybridization; D020641:Polymorphism, Single Nucleotide; D044022:Receptor, Endothelin A / Q000235:genetics*; D014652:Vascular Diseases / Q000175:diagnosis / Q000235:genetics; D044465:White People
D015342:DNA Probes; D019332:Endothelin-1; D019333:Endothelin-2; D044022:Receptor, Endothelin A