Multiplexed analyte and oligonucleotide detection on microarrays using several redox enzymes in conjunction with electrochemical detection.
We show that multiple enzyme tags may be used in an immunoassay format or for the detection of sequence-specific DNA on microarrays. The assays may be multiplexed and monitored under separate solution and voltage differences. Thus, the detection method relies on an electrochemical detection format, whereby multiple enzymes can be sensed. In our case we utilize horseradish peroxidase, laccase, and glucose dehydrogenase as enzymes attached to specific antibodies or to streptavidin.
Dill
Kilian
K
CombiMatrix Corporation, 6500 Harbour Heights Parkway, Suite 301, Mukilteo, WA 98275, USA. kilian@combimatrix.com
Ghindilis
Andrey
A
Schwarzkopf
Kevin
K
D000906:Antibodies / Q000737:chemistry*; D004563:Electrochemistry; D020411:Oligonucleotide Array Sequence Analysis; D010088:Oxidoreductases / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
D000906:Antibodies; D019809:Streptavidin; D010088:Oxidoreductases