Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays.
The PDZ domain is a protein-protein interaction module that interacts with a C-terminal short peptide motif in its binding partners. A variety of methods have been used to study PDZ domain interactions. This chapter details the two methods most commonly used in the analysis of PDZ interactions: yeast two-hybrid and coimmunoprecipitation assays. In addition, we discuss the features that must be considered for an efficient analysis of PDZ interactions.
Lee
Hyun Woo
HW
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon.
Ko
Jaewon
J
Kim
Eunjoon
E
D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D011993:Recombinant Fusion Proteins