title: Survival of Vibrio fluvialis in seawater under starvation conditions.
abstract: The viability of Vibrio fluvialis in seawater microcosms, with and without sediment was investigated. The strain survived as culturable bacteria for at least 1 year and the expression of its virulence factors was maintained. In microcosms containing sediment Vibrio fluvialis was more stable. Viable but nonculturable (VBNC) cells of Vibrio fluvialis were able to resuscitate to the culturable state up to 6 years of incubation in marine sediment. These cells recuperate their initial biochemical characteristics after 3 months of incubation in marine broth. Amplified 16S ribosomal DNA (rDNA) restriction analysis (ARDRA) was used to confirm that it is the same strain of Vibrio fluvialis which resists in all microcosms during a long period of time.
authors.affiliation: Laboratoire d'Analyse et de Contrôle des Polluants Chimiques et Microbiologiques de l'Environnement, Faculté de Pharmacie de Monastir- Rue Avicenne, Monastir, Tunisia. Nakbi_amel@yahoo.fr
authors.forename: Ben Kahla-Nakbi
authors.initials: BK
authors.lastname: Amel
authors.forename: Besbes
authors.initials: B
authors.lastname: Amine
authors.forename: Bakhrouf
authors.initials: B
authors.lastname: Amina
mesh_terms: D015169:Colony Count, Microbial; D019015:Geologic Sediments / Q000382:microbiology*; D050296:Microbial Viability; D021521:Ribotyping / Q000379:methods; D012623:Seawater / Q000382:microbiology*; D014733:Vibrio / Q000254:growth & development / Q000502:physiology*; D037521:Virulence Factors / Q000096:biosynthesis
chemical_list: D037521:Virulence Factors