Study of vertical transmission of fowl adenoviruses.
The vertical transmission of fowl adenoviruses (FAdVs) was studied by polymerase chain reaction (PCR) and virus isolation. Liver, spleen, kidney, and bursa of Fabricius were collected from 60 chicks 1 d old representing progenies hatched to 6 broiler breeder flocks in 6 geographically different premises in Ontario, Canada. The presence of FAdV DNA sequences was detected by PCR with the use of primers specific for the conserved pVI gene of FAdV-9 in 58 (24%) of the 240 samples tested. All samples from 1 flock were negative for FAdV sequences, and only a few samples were positive in 3 flocks, whereas 32% and 72% of the samples from the other 2 flocks were positive. Testing of 1 sample with primers designed to amplify the L1 region of the hexon protein gene and amino acid sequence analysis of the PCR product indicated that the sequences were similar to serotype-8a FAdV sequences. No fowl adenoviruses were isolated in chicken hepatoma cells from any of the 30 samples inoculated. These findings imply that vertical transmission and establishment of latent infection with FAdVs can occur in chickens.
Grgić
Helena
H
Department of Pathobiology, Ontario Veterinary College, University of Guelph.
Philippe
Cynthia
C
Ojkić
Davor
D
Nagy
Eva
E
D000257:Adenoviridae Infections / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D025681:Fowl adenovirus A / Q000145:classification / Q000302:isolation & purification; D005784:Gene Amplification; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D008969:Molecular Sequence Data; D009864:Ontario; D009928:Organ Specificity; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011201:Poultry Diseases / Q000635:transmission* / Q000821:virology
D017931:DNA Primers; D004279:DNA, Viral