Characterization of acid-tolerant H/CO-utilizing methanogenic enrichment cultures from an acidic peat bog in New York State.
Two methanogenic cultures were enriched from acidic peat soil using a growth medium buffered to c. pH 5. One culture, 6A, was obtained from peat after incubation with H(2)/CO(2), whereas culture NTA was derived from a 10(-4) dilution of untreated peat into a modified medium. 16S rRNA gene clone libraries from each culture contained one methanogen and two bacterial sequences. The methanogen 16S rRNA gene sequences were 99% identical with each other and belonged to the novel "R-10/Fen cluster" family of the Methanomicrobiales, whereas their mcrA sequences were 96% identical. One bacterial 16S rRNA gene sequence from culture 6A belonged to the Bacteroidetes and showed 99% identity with sequences from methanogenic enrichments from German and Russian bogs. The other sequence belonged to the Firmicutes and was identical to a thick rod-shaped citrate-utilizing organism isolated from culture 6A, the numbers of which decreased when the Ti (III) chelator was switched from citrate to nitrilotriacetate. Bacterial clones from the NTA culture clustered in the Delta- and Betaproteobacteria. Both cultures contained thin rods, presumably the methanogens, as the predominant morphotype, and represent a significant advance in characterization of the novel acidiphilic R-10 family methanogens.
Bräuer
Suzanna L
SL
Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Yashiro
Erika
E
Ueno
Norikiyo G
NG
Yavitt
Joseph B
JB
Zinder
Stephen H
SH
D000143:Acids / Q000494:pharmacology*; D000165:Acridine Orange; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D041963:Bacteroidetes; D020562:Betaproteobacteria; D003470:Culture Media / Q000737:chemistry; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020564:Deltaproteobacteria; D019605:Euryarchaeota / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000096:biosynthesis*; D017018:Methanomicrobiales; D008856:Microscopy, Fluorescence; D008857:Microscopy, Interference; D008858:Microscopy, Phase-Contrast; D009518:New York; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013194:Staining and Labeling
D000143:Acids; D000900:Anti-Bacterial Agents; D003470:Culture Media; D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000165:Acridine Orange; D008697:Methane