The inhibitory effects of eckol and dieckol from Ecklonia stolonifera on the expression of matrix metalloproteinase-1 in human dermal fibroblasts.
In order to develop new anti-photoaging agents, we examined the inhibitory effects of 29 seaweed extracts on transcriptional activities of NF-kappaB and AP-1, and MMP-1 expression. The extracts from 3 species of Alariaceae, Eisenia bicyclis, Ecklonia cava and Ecklonia stolonifera, have showed strong inhibition of both NF-kappaB and AP-1 reporter activity, which were well correlated with their abilities to inhibit MMP-1 expression. In addition, MMP-1 expression was dramatically attenuated by treatment with eckol or dieckol which were purely isolated from E. stolonifera, indicating that these compounds are active principles to inhibit MMP-1 expression in human dermal fibroblasts. Taken together, our data demonstrate the inhibitory effect of eckol and dieckol from Ecklonia species on MMP-1 expression in human dermal fibroblasts and provide a possibility to develop as an agent for the prevention and treatment of skin aging.
Joe
Min-Jeong
MJ
Marine Biomedical Research Center, Kwandong University College of Medicine, Korea.
Kim
Su-Nam
SN
Choi
Hye-Young
HY
Shin
Woon-Seob
WS
Park
Gab-Man
GM
Kang
Dong-Won
DW
Kim
Yong Kee
YK
D001483:Base Sequence; D001572:Benzofurans / Q000494:pharmacology*; D015153:Blotting, Western; D002478:Cells, Cultured; D017931:DNA Primers; D004147:Dioxins / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D000459:Phaeophyceae / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
D001572:Benzofurans; D017931:DNA Primers; D004147:Dioxins; C503840:dieckol; C060311:eckol; D020781:Matrix Metalloproteinase 1