Nasal absorption of metoclopramide from different Carbopol 981 based formulations: In vitro, ex vivo and in vivo evaluation.
There is a need for nasal drug delivery of metoclopramide HCI (MTC) in specific patient populations where the use of commercially available intravenous and oral dosage forms may be inconvenient and/or unfeasible. In this perspective, nasal dosage forms (solution, gel and lyophilized powder) of MTC were prepared by using a mucoadhesive polymer Carbopol 981 (CRB 981). The drug release studies of formulations were performed by using a modified horizontal diffusion chamber with cellulose membrane and excised cattle nasal mucosa as diffusion barriers. After the ex vivo experiments, the morphological appearances of the nasal mucosa were analyzed with the light microscopic studies. In vivo experiments were carried on sheep model. The release of MTC from solution and powder formulations was found higher than gel formulation (p < 0.05) and no severe damage was found on the integrity of nasal mucosa after ex vivo experiments. The penetration enhancing effect of dimethyl-beta-cyclodextrin (DM-beta-CD) used in powder formulations was observed in ex vivo and in vivo experiments. In contrast to in vitro and ex vivo experiments the nasal bioavailability of gel formulation was found higher than those of the solution and powder (p < 0.05) and might represent a promising novel tool for the systemic delivery of MTC.
Tas
Cetin
C
Gülhane Military Medical Academy, Department of Pharmaceutical Technology, Etlik-Ankara, Turkey.
Ozkan
Cansel Kose
CK
Savaser
Ayhan
A
Ozkan
Yalcin
Y
Tasdemir
Umut
U
Altunay
Hikmet
H
D000042:Absorption / Q000502:physiology; D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001682:Biological Availability; D018492:Dopamine Antagonists / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005782:Gels; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008787:Metoclopramide / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D009297:Nasal Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D010539:Permeability; D011208:Powders; D012756:Sheep; D012996:Solutions; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000493:pharmacokinetics
D000180:Acrylic Resins; C516894:Carbopol 981; D018492:Dopamine Antagonists; D005782:Gels; D011208:Powders; D012996:Solutions; D047392:beta-Cyclodextrins; C038119:heptakis(2,6-O-dimethyl)beta-cyclodextrin; D008787:Metoclopramide