Micropatterning polyvinyl alcohol as a biomimetic material through soft lithography with cell culture.
Integrating inorganic materials with specific biological systems requires a complex set of properties that can mimic physiological function to create microchip devices. Several factors in the material including the spatial location of the cellular attachment heavily influence the control of this functionality and must be dictated to parallel in vivo characteristics. In this work, we demonstrate polyvinyl alcohol (PVA) for use in emulating these properties to produce a highly robust and functional biomaterial as a microchip. We show the utility of PVA for cellular patterning using soft lithography. Based on these results, PVA can be applied in a diversity of areas including tissue engineering, biomimetics, and cellular micropatterning.
Cheng
Chao-Min
CM
Department of Mechanical and Biomedical Engineering, Carnegie Mellon University, Scaife Hall, 5000 Forbes Avenue, Pittsburgh, PA 15213, USA.
LeDuc
Philip R
PR
D000818:Animals; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D020100:Hydrogels / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D023822:Tissue Engineering; D014867:Water / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D014867:Water; D011142:Polyvinyl Alcohol