In vivo three-dimensional photoacoustic tomography of a whole mouse head.
An in vivo photoacoustic imaging system was designed and implemented to image the entire small animal head. A special scanning gantry was designed to enable in vivo imaging in coronal cross sections with high contrast and good spatial resolution for the first time to our knowledge. By use of a 2.25 MHz ultrasonic transducer with a 6 mm diameter active element, an in-plane radial resolution of approximately 312 microm was achieved. Deeply seated arterial and venous vessels in the head measuring up to 1.7 cm in diameter were simultaneously imaged in vivo with 804 nm wavelength laser excitation of photoacoustic waves.
Song
Kwang Hyun
KH
Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA. khsong@tamu.edu
Stoica
George
G
Wang
Lihong V
LV
D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006257:Head / Q000033:anatomy & histology*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods / Q000662:veterinary*; D051379:Mice; D033401:Microscopy, Acoustic / Q000295:instrumentation* / Q000379:methods / Q000662:veterinary*; D010865:Pilot Projects; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods / Q000662:veterinary*