[Changes of peripheral blood T lymphocytes, IFN-gamma, TNF-alpha and IL-4 in rats infected by Toxoplasma gondii].
OBJECTIVE
To observe the changes of peripheral blood T lymphocytes, IFN-gamma, TNF-alpha and IL.4 in rats infected by T gondii.


METHODS
48 Sprague-Dawley(SD) rats were intra-abdominally injected with 2 x 10(5)/L of cellulose purified living tachyzoites in 2 ml and randomly divided into 8 groups Six rat was intra-abdominally injected 2 ml of saline as control and 4 rats were remained as normal control. Peripheral blood was collected and the level of IFN-gamma, TNF-alpha, IL-4 was analyzed by ELISA on day 1, 3, 7, 14, 28, 35, 42, 60.


RESULTS
Level of IFN-gamma (6.73 pg/nil) and IL-4 (6.91 pg/ml) increased in experimental rats on day 7 (P < 0.05) and maintained. Level of TNF-alpha (14.37 pg/ml) increased in experimental rats on day 28 (P < 0.05), and that of CD8+ T lymphocytes (14.22%) was signficantly lower than that in control (23.08%) (P < 0.05) and recovered on day 28. No considerable change was observed on the level of CD4 T lymphocytes.


CONCLUSIONS
The level of CD8 T lymphocytes, IFN-gamma, TNF-alpha and IL-4 in the rat can be affected by the infection of T. gondii and the level of CD4+ T lymphocytes shows no change.
Wang
Chun-li
CL
Department of Infection, PLA 101 Hospital, Wuxi 214044, China.
Zhou
Yong-hua
YH
Liu
Jian-hu
JH
D000818:Animals; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D006790:Host-Parasite Interactions; D007371:Interferon-gamma / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood*; D018655:Lymphocyte Count; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014122:Toxoplasma / Q000502:physiology*; D014124:Toxoplasmosis, Animal / Q000097:blood* / Q000276:immunology / Q000469:parasitology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma