title: Association of Gln222Arg polymorphism in the deoxyribonuclease I (DNase I) gene with myocardial infarction in Japanese patients.
abstract: OBJECTIVE
  We have recently reported that serum deoxyribonuclease I (DNase I) activity, which may be involved in apoptosis, increases abruptly in the early phase of acute myocardial infarction (MI) [Kawai Y, Yoshida M, Arakawa K, Kumamoto T, Morikawa N, Masamura K, Tada H, Ito S, Hoshizaki H, Oshima S, Taniguchi K, Terasawa H, Miyamori I, Kishi K, Yasuda T. Diagnostic use of serum deoxyribonuclease I activity as a novel early-phase marker in acute myocardial infarction. Circulation 2004;109:2398-2400]. Death of vascular smooth muscle cells, in part because of apoptosis, is postulated to heighten susceptibility to disruption of vulnerable plaque, resulting in onset of MI. The present study evaluated the possibility that Gln222Arg polymorphism of the DNase I gene may be one of the factors involved in predisposition to MI.
  
  
  RESULTS
  We assessed 611 Japanese patients: 311 with MI and 300 with stable angina pectoris (AP). Three common phenotypes determined by two common codominant alleles, DNASE1*1 and *2, whose corresponding gene products exhibit different properties, were found in these patient groups. The prevalence of DNASE1*2 was significantly higher in patients with MI than in those with AP (0.543 vs. 0.428, P < 0.001), being confirmed by phenotyping of the second study population. Multiple logistic regression analysis showed that the odds ratio of DNASE1*2 was 1.51 [95% confidence interval (CI) 1.04-2.18]. The association of the DNASE1*2 allele with MI was statistically significant, being independent of other conventional risk factors.
  
  
  CONCLUSIONS
  Our data demonstrate that Gln222Arg polymorphism in the DNase I gene is associated with MI in the Japanese patients.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Kumamoto
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kawai
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Arakawa
authors.forename: Norihiro
authors.initials: N
authors.lastname: Morikawa
authors.forename: Jun
authors.initials: J
authors.lastname: Kuribara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tada
authors.forename: Koichi
authors.initials: K
authors.lastname: Taniguchi
authors.forename: Ryozo
authors.initials: R
authors.lastname: Tatami
authors.forename: Isamu
authors.initials: I
authors.lastname: Miyamori
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kominato
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kishi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yasuda
mesh_terms: D000368:Aged; D000787:Angina Pectoris / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D003850:Deoxyribonuclease I / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D012044:Regression Analysis; D012307:Risk Factors
chemical_list: D003850:Deoxyribonuclease I