title: [High effectiveness of the method with cefpirome in detection of extended-spectrum beta-lactamases in different species of gram-negative bacilli].
abstract: Gram-negative bacilli were examined for ESBL production by using four methods: double-disc synergy diffusion test (DDST), and three tests of combined discs with cefpodoxime, ceftazidime and cefotaxime alone and the same cephalosporins with clavulanic acid. Strains determined as ESBL-negative with all these tests were examined by using fifth method with cefpirome. 47,5% from 178 negative in other methods strains, appeared ESBL-positive in this test. The examined strains belonged to 16 different species. Most of them were Enterobacter cloaceae, Serratia marcescens, Pseudomonas aeruginosa and Acinetobacter baumanii. It seems that the combined discs method with cefpirome may be usefull for phenotypic detection of ESBL producing bacteria also in the case of strains where ESBL production is camouflaged with derepressed chromosomal AmpC beta-lactamases.
authors.affiliation: Katedra i Zakład Mikrobiologii Lekarskiej AM w Warszawie.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Młynarczyk
authors.forename: Andrzej
authors.initials: A
authors.lastname: Młynarczyk
authors.forename: Agata
authors.initials: A
authors.lastname: Bilewska
authors.forename: Agata
authors.initials: A
authors.lastname: Dukaczewska
authors.forename: Cezary
authors.initials: C
authors.lastname: Goławski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kicman
authors.forename: Julia
authors.initials: J
authors.lastname: Pupek
authors.forename: Iwona
authors.initials: I
authors.lastname: Serafin
authors.forename: Mirosław
authors.initials: M
authors.lastname: Luczak
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques / Q000379:methods; D001431:Bacteriological Techniques / Q000379:methods; D002439:Cefotaxime / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology*; D019818:Clavulanic Acid / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests / Q000379:methods; D013045:Species Specificity; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis*; D000097572:Cefpirome
chemical_list: D000900:Anti-Bacterial Agents; D002439:Cefotaxime; D002511:Cephalosporins; D019818:Clavulanic Acid; D001618:beta-Lactamases; D000097572:Cefpirome