Modulatory efficacy of green tea polyphenols on glycoconjugates and immunological markers in 4-Nitroquinoline 1-oxide-induced oral carcinogenesis-A therapeutic approach.
Green tea polyphenols (GTP) has been used as a chemopreventive agent world wide against chemically induced cancer. The present study is aimed to understand the therapeutic action of GTP on glycoconjugates and immunological markers in 4-Nitroquinoline 1-oxide (4-NQO)-induced oral cancer over a period of 30 days at 200mg/kg, p.o., Oral cancer was induced by painting 4-NQO for 8 weeks followed by administration of GTP after 22 weeks, for 30 days. Glycoconjugates such as hexose, hexosamine, sialicacid, fucose and mucoprotein were analysed. Expression of glycoconjugates was examined through histology and SDS-PAGE. Immunological markers such as circulating immune complex and mast cell density were studied. Oral cancer-induced animals showed a significant increase in levels of glycoconjugates and its expression, similar to that observed for immunological markers. Treatment with GTP altered the expression of glycoconjugates as well as immunological markers. The results suggest that GTP modulates both the expression of glycoconjugates and immunological markers resulting in regression of oral cancer.
Srinivasan
Periasamy
P
Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, Tamilnadu, India.
Sabitha
Kuruvimalai Ekambaram
KE
Shyamaladevi
Chennam Srinivasulu
CS
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use; D028241:Camellia sinensis / Q000737:chemistry*; D005419:Flavonoids / Q000627:therapeutic use*; D005966:Glucuronidase / Q000378:metabolism; D006001:Glycoconjugates / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism; D007136:Immunoglobulins / Q000097:blood*; D008297:Male; D009055:Mouth / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D009088:Mucoproteins / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D010636:Phenols / Q000627:therapeutic use*; D010740:Phospholipases / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar; D014059:Tongue / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014062:Tongue Neoplasms / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D006001:Glycoconjugates; D006601:Hexoses; D007136:Immunoglobulins; D009088:Mucoproteins; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D015112:4-Nitroquinoline-1-oxide; D010740:Phospholipases; D000135:Acid Phosphatase; D001616:beta-Galactosidase; D005966:Glucuronidase; D019158:N-Acetylneuraminic Acid