Random amplified polymorphic DNA analysis of genetically modified organisms.
Randomly amplified polymorphic DNA (RAPD) was used to analyzed 78 samples comprises of certified reference materials (soya and maize powder), raw seeds (soybean and maize), processed food and animal feed. Combination assay of two arbitrary primers in the RAPD analysis enable to distinguish genetically modified organism (GMO) reference materials from the samples tested. Dendrogram analysis revealed 13 clusters at 45% similarity from the RAPD. RAPD analysis showed that the maize and soybean samples were clustered differently besides the GMO and non-GMO products.
Yoke-Kqueen
Cheah
C
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. ykcheah@medic.upm.edu.my
Radu
Son
S
D005504:Food Analysis / Q000379:methods; D030841:Food, Genetically Modified; D030821:Plants, Genetically Modified / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012016:Reference Values; D013025:Glycine max / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*