[Preparation and biocompatibility of a novel cornea scaffold material].
A novel cornea tissue scaffold material was prepared with N-vinly pyrrolidome (NVP) and a biodegradable crosslinking agent by radical polymerization, using azoisobutyronitrile (AIBN) as initiator. Water absorption test and contact angle measure were conducted, and the degradation process of material was investigated. The biocompatibility evaluation was carried out by implantation of material in the rabbits, and by cell culture. The water absorption was over 104%, the contact angle was lower than 41degrees, and the degradation speed in vitro kept steady. The results of implantation in the rabbits showed that the material was almost degraded 3 months later and lots of collagen and cornea stroma cells appeared in it,but there was no inflammation around it. The result of epithelial cells culture showed that the cells conglutinated on the material, but no remarkable cytotoxicity was noted.
Jiao
Yanpeng
Y
Institute of Life Science and Technology, Jinan University, Guanzhou 510632, China.
Li
Lihua
L
Ding
Yong
Y
Zhou
Changren
C
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D003315:Cornea / Q000166:cytology*; D019573:Epithelium, Corneal / Q000166:cytology; D008422:Materials Testing; D009570:Nitriles / Q000737:chemistry*; D019736:Prostheses and Implants; D011760:Pyrrolidinones / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering
D001672:Biocompatible Materials; D009570:Nitriles; D011760:Pyrrolidinones; C004526:azobis(isobutyronitrile)