title: Linkage disequilibrium mapping in domestic dog breeds narrows the progressive rod-cone degeneration interval and identifies ancestral disease-transmitting chromosome.
abstract: Canine progressive rod-cone degeneration (prcd) is a retinal disease previously mapped to a broad, gene-rich centromeric region of canine chromosome 9. As allelic disorders are present in multiple breeds, we used linkage disequilibrium (LD) to narrow the approximately 6.4-Mb interval candidate region. Multiple dog breeds, each representing genetically isolated populations, were typed for SNPs and other polymorphisms identified from BACs. The candidate region was initially localized to a 1.5-Mb zero recombination interval between growth factor receptor-bound protein 2 (GRB2) and SEC14-like 1 (SEC14L). A fine-scale haplotype of the region was developed, which reduced the LD interval to 106 kb and identified a conserved haplotype of 98 polymorphisms present in all prcd-affected chromosomes from 14 different dog breeds. The findings strongly suggest that a common ancestor transmitted the prcd disease allele to many of the modern dog breeds and demonstrate the power of the LD approach in the canine model.
authors.affiliation: James A. Baker Institute, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Orly
authors.initials: O
authors.lastname: Goldstein
authors.forename: Barbara
authors.initials: B
authors.lastname: Zangerl
authors.forename: Sue
authors.initials: S
authors.lastname: Pearce-Kelling
authors.forename: Duska J
authors.initials: DJ
authors.lastname: Sidjanin
authors.forename: James W
authors.initials: JW
authors.lastname: Kijas
authors.forename: Jeanette
authors.initials: J
authors.lastname: Felix
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Acland
authors.forename: Gustavo D
authors.initials: GD
authors.lastname: Aguirre
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002874:Chromosome Mapping / Q000662:veterinary*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics* / Q000473:pathology; D004285:Dogs / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005828:Genetics, Population; D023281:Genomics; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008297:Male; D010802:Phylogeny; D012162:Retinal Degeneration / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary