Filamentous-haemagglutinin-like protein genes encoded on a plasmid of Moraxella bovis.
The complete nucleotide sequence of a plasmid, pMBO-1, from Moraxella bovis strain Epp63 was determined. We identified 30 open reading frames (ORFs) encoded by the 44,215bp molecule. Two large ORFs, flpA and flpB, encoding proteins with similarity to Bordetella pertussis filamentous haemagglutinin (FHA), were identified on the same plasmid. The gene for a specific accessory protein (Fap), which may play a role in the secretion of Flp protein, was also identified. Reverse transcriptase PCR analysis of total RNA isolated from M. bovis Epp63 indicated that the flpA, flpB, and fap genes are all transcribed. Southern blot analysis indicated that the flp and fap genes are present in other clinical isolates of geographically diverse M. bovis.
Kakuda
Tsutomu
T
Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University, Higashi 23, 35-1, Towada, Aomori 034-8628, Japan. kakuda@vmas.kitasato-u.ac.jp
Sarataphan
Nopporn
N
Tanaka
Tetsuya
T
Takai
Shinji
S
D000818:Animals; D001426:Bacterial Proteins; D001483:Base Sequence; D015139:Blotting, Southern / Q000662:veterinary; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006388:Hemagglutinins / Q000737:chemistry* / Q000235:genetics*; D007140:Immunoglobulin Fab Fragments; D008969:Molecular Sequence Data; D016955:Moraxella bovis / Q000235:genetics*; D016366:Open Reading Frames; D010957:Plasmids; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014157:Transcription Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C119770:FlpA protein, bacteria; D006388:Hemagglutinins; D007140:Immunoglobulin Fab Fragments; D014157:Transcription Factors