Cultured early goat embryos and cells are susceptible to infection with caprine encephalitis virus.
Zona-pellucida-free embryos at 8-16 cell stage were co-cultured for 6 days in an insert over a mixed cell monolayer infected with CAEV-pBSCA. Embryos were washed and transferred to an insert on CAEV indicator goat synovial membrane cells for 6 h, then they were washed and cultivated in B2 Ménézo for 24 h, finally, embryo cells were dissociated and cultivated on a feeder monolayer for 8 days. After 5 weeks, multinucleated giant cells typical of CAEV infection were observed in indicator GSM cell monolayers. In the acellular medium, the early embryonic cells produced at least 10(3.25) TCID50/ml over 24 h. The monolayer of cultivated embryonic cells developed cytopathic lesions within 8 days, and CAEV RNA, CAEV proviral DNA and protein p28 of the capsid were detected. All of these results clearly demonstrate that caprine early embryonic cells are susceptible to infection with CAEV and that infection with this virus is productive.
Ali Al Ahmad
M Z
MZ
Department of Research into the Health Risk and Biotechnology of Reproduction ENVN/DGER, National Veterinary School, BP 40706, 44307 Nantes Cedex 03, France.
Fieni
F
F
Guiguen
F
F
Larrat
M
M
Pellerin
J L
JL
Roux
C
C
Chebloune
Y
Y
D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*; D036022:Capsid Proteins / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques; D004279:DNA, Viral / Q000235:genetics; D004622:Embryo, Mammalian / Q000821:virology; D016679:Genome, Viral / Q000235:genetics; D006041:Goats; D007150:Immunohistochemistry; D016180:Lentivirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014779:Virus Replication
D036022:Capsid Proteins; D004279:DNA, Viral