Immunohistochemical expression of heparan sulfate correlates with stromal cell proliferation in breast phyllodes tumors.
Phyllodes tumors are fibroepithelial neoplasms typified by stromal proliferation. We have previously shown the role of pathologic parameters and the prognostic significance of p53 and CD117 protein expression in these tumors. In this study, we evaluated the expression of heparan sulfate, which has been implicated in many biological processes such as cell adhesion, embryogenesis, and tumorigenesis (including malignant transformation of mammary cells) in 232 breast phyllodes tumors. We used a monoclonal antibody, 10E4, to examine the localization of heparan sulfate in phyllodes tumors by immunohistochemistry. The immunoreactivity of both epithelial and stromal components was examined and analyzed with pathological parameters and other immunohistochemical markers, including p53, MIB1, bcl2, and CD117. Stromal 10E4 expression was significantly associated with tumor grade, stromal p53, and MIB1 expression in proliferating cells, suggesting that heparan sulfate may participate in malignant tumor growth.
Koo
Chuay-Yeng
CY
Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Bay
Boon-Huat
BH
Lui
Philip Chi-Wai
PC
Tse
Gary Man-Kit
GM
Tan
Puay-Hoon
PH
Yip
George Wai-Cheong
GW
D000974:Antibodies, Antinuclear / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000737:chemistry / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006497:Heparan Sulfate / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D003557:Phyllodes Tumor / Q000737:chemistry* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D012189:Retrospective Studies; D017154:Stromal Cells / Q000737:chemistry / Q000473:pathology; D046888:Tissue Array Analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D006497:Heparan Sulfate; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D019009:Proto-Oncogene Proteins c-kit; D016159:Tumor Suppressor Protein p53; C440408:MIB-1 antibody