title: Aberrant expression of ID2, a suppressor of B-cell-specific gene expression, in Hodgkin's lymphoma.
abstract: The global loss of B-cell-specific gene expression is a distinctive feature of the Hodgkin-Reed/Sternberg (HRS) cells of classical Hodgkin's lymphoma (HL). The reasons for this loss remained largely unknown as transcription factors with pleiotropic effects on B-cell-specific gene expression, namely E2A, EBF, and PAX5, are present in primary HRS cells. We show here that ID2, which can inactivate E2A and perhaps PAX5, is not detectable in normal B cells but is strongly and uniformly expressed in HRS cells of all cases of classical HL. Recurrent chromosomal gains of the ID2 gene might contribute to this aberrant expression. Co-immunoprecipitation of E2A with ID2 from HRS-derived cell lines together with the high amount of ID2 relative to the B-cell transcription factors E2A and PAX5 in HRS-derived cell lines and primary HRS cells indicated that aberrant ID2 expression contributes significantly to the loss of the B-cell-specific gene expression in HRS cells. ID2 was also expressed in lymphocyte-predominance HL, mediastinal large B-cell, diffuse large B-cell, and Burkitt's lymphoma, where lower amounts of ID2 relative to E2A and PAX5 compared with HRS cells might prevent a global down-regulation of B-cell-specific genes and ID2 may contribute to lymphomagenesis in other ways.
authors.affiliation: Senckenberg Institute for Pathology, University of Frankfurt, Frankfurt.
authors.forename: Christoph
authors.initials: C
authors.lastname: Renné
authors.forename: Jose Ignacio
authors.initials: JI
authors.lastname: Martin-Subero
authors.forename: Maren
authors.initials: M
authors.lastname: Eickernjäger
authors.forename: Martin-Leo
authors.initials: ML
authors.lastname: Hansmann
authors.forename: Ralf
authors.initials: R
authors.lastname: Küppers
authors.forename: Reiner
authors.initials: R
authors.lastname: Siebert
authors.forename: Andreas
authors.initials: A
authors.lastname: Bräuninger
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002051:Burkitt Lymphoma / Q000473:pathology; D051925:CD79 Antigens / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm / Q000235:genetics*; D015894:Genome, Human / Q000235:genetics; D006689:Hodgkin Disease / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051797:Inhibitor of Differentiation Protein 2 / Q000235:genetics* / Q000378:metabolism*; D051757:PAX5 Transcription Factor / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016539:Reed-Sternberg Cells / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D051925:CD79 Antigens; D051797:Inhibitor of Differentiation Protein 2; D051757:PAX5 Transcription Factor; D012333:RNA, Messenger; C497450:ID2 protein, human; C407909:TCF3 protein, human