Sample work-up by column techniques.
A review is given of the extraction and purification of biological samples by filtration through lipophilic neutral and ion-exchange dextran gels and their derivatives. Liquid-gel extraction, reversed-phase liquid chromatography and ligand-exchange chromatography are also discussed. The applications of Sephadex and Lipidex gels are reviewed, with special reference to extractions from biological fluids and solid samples and the extraction of metabolites. A number of selective isolation procedures are reviewed for estrogens, lignans, isoflavanes, isoflavones, catechol estrogens, ketonic compounds and ethynyl steroids.
Sjövall
J
J
Department of Physiological Chemistry and Department of Clinical Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden.
Axelson
M
M