title: CD8+ T-Cell responses to Trypanosoma cruzi are highly focused on strain-variant trans-sialidase epitopes.
abstract: CD8+ T cells are crucial for control of a number of medically important protozoan parasites, including Trypanosoma cruzi, the agent of human Chagas disease. Yet, in contrast to the wealth of information from viral and bacterial infections, little is known about the antigen specificity or the general development of effector and memory T-cell responses in hosts infected with protozoans. In this study we report on a wide-scale screen for the dominant parasite peptides recognized by CD8+ T cells in T. cruzi-infected mice and humans. This analysis demonstrates that in both hosts the CD8+ T-cell response is highly focused on epitopes encoded by members of the large trans-sialidase family of genes. Responses to a restricted set of immunodominant peptides were especially pronounced in T. cruzi-infected mice, with more than 30% of the CD8+ T-cell response at the peak of infection specific for two major groups of trans-sialidase peptides. Experimental models also demonstrated that the dominance patterns vary depending on the infective strain of T. cruzi, suggesting that immune evasion may be occurring at a population rather than single-parasite level.
authors.affiliation: Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, United States of America.
authors.forename: Diana L
authors.initials: DL
authors.lastname: Martin
authors.forename: D Brent
authors.initials: DB
authors.lastname: Weatherly
authors.forename: Susana A
authors.initials: SA
authors.lastname: Laucella
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Cabinian
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Crim
authors.forename: Susan
authors.initials: S
authors.lastname: Sullivan
authors.forename: Mark
authors.initials: M
authors.lastname: Heiges
authors.forename: Sarah H
authors.initials: SH
authors.lastname: Craven
authors.forename: Charles S
authors.initials: CS
authors.lastname: Rosenberg
authors.forename: Matthew H
authors.initials: MH
authors.lastname: Collins
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sette
authors.forename: Miriam
authors.initials: M
authors.lastname: Postan
authors.forename: Rick L
authors.initials: RL
authors.lastname: Tarleton
mesh_terms: D000328:Adult; D000818:Animals; D001118:Argentina; D001938:Brazil; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D014355:Chagas Disease / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D014644:Genetic Variation; D016678:Genome; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008285:Major Histocompatibility Complex; D051379:Mice; D008810:Mice, Inbred C57BL; D009439:Neuraminidase / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000235:genetics* / Q000276:immunology*
chemical_list: D007527:Isoenzymes; D009439:Neuraminidase