title: Stability of thermostable alkaline protease from Bacillus licheniformis RP1 in commercial solid laundry detergent formulations.
abstract: The stability of crude extracellular protease produced by Bacillus licheniformis RP1, isolated from polluted water, in various solid laundry detergents was investigated. The enzyme had an optimum pH and temperature at pH 10.0-11.0 and 65-70 degrees C. Enzyme activity was inhibited by PMSF, suggesting that the preparation contains a serine-protease. The alkaline protease showed extreme stability towards non-ionic (5% Tween 20% and 5% Triton X-100) and anionic (0.5% SDS) surfactants, which retained 100% and above 73%, respectively, of its initial activity after preincubation 60 min at 40 degrees C. The RP1 protease showed excellent stability and compatibility with a wide range of commercial solid detergents at temperatures from 40 to 50 degrees C, suggesting its further application in detergent industry. The enzyme retained 95% of its initial activity with Ariel followed by Axion (94%) then Dixan (93.5%) after preincubation 60 min at 40 degrees C in the presence of 7 mg/ml of detergents. In the presence of Nadhif and New Det, the enzyme retained about 83.5% of the original activity. The effects of additives such as maltodextrin, sucrose and PEG 4000 on the stability of the enzyme during spray-drying and during subsequent storage in New Det detergent were also examined. All additives tested enhanced stability of the enzyme.
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie-Ecole Nationale d'Ingénieurs de Sfax, Sfax, Tunisia.
authors.forename: Alya
authors.initials: A
authors.lastname: Sellami-Kamoun
authors.forename: Anissa
authors.initials: A
authors.lastname: Haddar
authors.forename: Nedra El-Hadj
authors.initials: Nel-H
authors.lastname: Ali
authors.forename: Basma
authors.initials: B
authors.lastname: Ghorbel-Frikha
authors.forename: Safia
authors.initials: S
authors.lastname: Kanoun
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology*; D010450:Endopeptidases / Q000737:chemistry* / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D017830:Octoxynol / Q000494:pharmacology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013696:Temperature; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D004791:Enzyme Inhibitors; D011136:Polysorbates; D012967:Sodium Dodecyl Sulfate; D010664:Phenylmethylsulfonyl Fluoride; D017830:Octoxynol; D010450:Endopeptidases; C087822:alkaline protease