Porcine OGN and ASPN: mapping, polymorphisms and use for quantitative trait loci identification for growth and carcass traits in a Meishan x Piétrain intercross.
The porcine orthologues of human chromosome HSA9q22.31 genes osteoglycin (OGN) and asporin (ASPN) were mapped to porcine chromosome SSC3 using linkage analysis and a somatic cell hybrid panel. This mapping was refined to SSC3q11 using fluorescence in situ hybridization. These results confirm the existence of a small conserved synteny group between SSC3 and HSA9. Polymorphisms were revealed in both genes, including a pentanucleotide microsatellite (SCZ003) in OGN and two single nucleotide polymorphisms (AM181682.1:g.780G>T and AM181682.1:g.825T>C) in ASPN. The two genes were included in a set of markers for quantitative trait loci (QTL) mapping on SSC3 in the Hohenheim Meishan x Piétrain F2 family. Major QTL for growth and carcass traits were centred in the ASPN-SW902 region.
Stratil
A
A
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 277 21 Libechov, Czech Republic. stratil@iapg.cas.cz
Van Poucke
M
M
Bartenschlager
H
H
Knoll
A
A
Yerle
M
M
Peelman
L J
LJ
Kopecný
M
M
Geldermann
H
H
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D011509:Proteoglycans / Q000235:genetics*; D040641:Quantitative Trait Loci; D013552:Swine / Q000235:genetics* / Q000254:growth & development; D026801:Synteny
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C063488:OGN protein, human; D011509:Proteoglycans