Brassinosteroids regulate dissociation of BKI1, a negative regulator of BRI1 signaling, from the plasma membrane.
Brassinosteroids, the steroid hormones of plants, are perceived at the plasma membrane by a leucine-rich repeat receptor serine/threonine kinase called BRI1. We report a BRI1-interacting protein, BKI1, which is a negative regulator of brassinosteroid signaling. Brassinosteroids cause the rapid dissociation of BKI1-yellow fluorescent protein from the plasma membrane in a process that is dependent on BRI1-kinase. BKI1 is a substrate of BRI1 kinase and limits the interaction of BRI1 with its proposed coreceptor, BAK1, suggesting that BKI1 prevents the activation of BRI1.
Wang
Xuelu
X
Howard Hughes Medical Institute and Plant Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Chory
Joanne
J
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D060406:Brassinosteroids; D002462:Cell Membrane / Q000378:metabolism*; D002777:Cholestanols / Q000378:metabolism* / Q000494:pharmacology; D004268:DNA-Binding Proteins; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010766:Phosphorylation; D030821:Plants, Genetically Modified; D011485:Protein Binding; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D013260:Steroids, Heterocyclic / Q000378:metabolism* / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins; C456160:BES1 protein, Arabidopsis; C513107:BKI1 protein, Arabidopsis; D060406:Brassinosteroids; D002777:Cholestanols; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D013260:Steroids, Heterocyclic; D011494:Protein Kinases; C108270:BRI1 protein, Arabidopsis; C023623:brassinolide