title: The stringent response is required for Helicobacter pylori survival of stationary phase, exposure to acid, and aerobic shock.
abstract: The gastric pathogen Helicobacter pylori must adapt to fluctuating conditions in the harsh environment of the human stomach with the use of a minimal number of transcriptional regulators. We investigated whether H. pylori utilizes the stringent response, involving signaling through the alarmone (p)ppGpp, as a survival strategy during environmental stresses. We show that the H. pylori homologue of the bifunctional (p)ppGpp synthetase and hydrolase SpoT is responsible for all cellular (p)ppGpp production in response to starvation conditions. Furthermore, the H. pylori spoT gene complements the growth defect of Escherichia coli mutants lacking (p)ppGpp. An H. pylori spoT deletion mutant is impaired for stationary-phase survival and undergoes a premature transformation to a coccoid morphology. In addition, the spoT deletion mutant is unable to survive specific environmental stresses, including aerobic shock and acid exposure, which are likely to be encountered by this bacterium during infection and transmission.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
authors.forename: Kyle
authors.initials: K
authors.lastname: Mouery
authors.forename: Bethany A
authors.initials: BA
authors.lastname: Rader
authors.forename: Erin C
authors.initials: EC
authors.lastname: Gaynor
authors.forename: Karen
authors.initials: K
authors.lastname: Guillemin
mesh_terms: D000143:Acids; D000332:Aerobiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D006159:Guanosine Tetraphosphate / Q000378:metabolism; D016480:Helicobacter pylori / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D006867:Hydrolases / Q000235:genetics / Q000502:physiology*; D008025:Ligases / Q000235:genetics / Q000502:physiology*
chemical_list: D000143:Acids; D001426:Bacterial Proteins; D006159:Guanosine Tetraphosphate; D006867:Hydrolases; D008025:Ligases