CD45 is required for type I IFN production by dendritic cells.
CD45 is a leukocyte tyrosine phosphatase, essential for normal immune responses. We have studied the function of splenic dendritic cells of CD45(+/+), CD45(-/-), CD45RABC and CD45RO transgenic mice. We show that there are increased numbers of plasmacytoid dendritic cells in CD45(-/-) mice. DC of all mice are capable of responding to lymphocytic choriomeningitis virus (LCMV) infection by up-regulation of MHC and costimulatory molecules. DC of CD45(-/-) mice have an impaired capacity to produce type I interferons in response to LCMV infection in vivo. These data indicate that lack of CD45 expression in DC has a profound effect on their function. This is largely restored by CD45RABC or CD45RO transgenes.
Montoya
Maria
M
The Edward Jenner Institute for Vaccine Research, Compton, UK.
Dawes
Ritu
R
Reid
Delyth
D
Lee
Lian Ni
LN
Piercy
Jenny
J
Borrow
Persephone
P
Tchilian
Elma Z
EZ
Beverley
Peter C L
PC
D000818:Animals; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D007370:Interferon Type I / Q000096:biosynthesis*; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000378:metabolism*; D008216:Lymphocytic Choriomeningitis / Q000378:metabolism / Q000821:virology; D008217:Lymphocytic choriomeningitis virus / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D010641:Phenotype
D007370:Interferon Type I; D017493:Leukocyte Common Antigens