title: Simple, inexpensive, and precise paraffin tissue microarrays constructed with a conventional microcompound table and a drill grinder.
abstract: In 1998, paraffin tissue microarrays (PTMAs) as paraffin blocks containing up to 1,000 cylindrical paraffin tissue core biopsy specimens (PTCBs) for high-throughput molecular profiling of tumor specimens were introduced. PTCBs can be constructed using a manual tissue puncher/arrayer (Beecher Instruments, Sun Prairie, WI; cost, at least $7,000). Furthermore, custom-built PTMAs such as the MaxArray are created by companies such as Zymed Laboratories (South San Francisco, CA; PTMA with 96 holes, about $900). In our search for a less expensive alternative, we constructed PTMAs with up to 558 PTCBs by using a drill grinder, a drill stand, and a microcompound table (Proxxon, Niersdorf, Germany; cost, <$300).
authors.affiliation: Institute of Pathology, University Clinic, Eberhard-Karls-University, Tuebingen, Germany.
authors.forename: Ulrich F
authors.initials: UF
authors.lastname: Vogel
authors.forename: Burkhard D
authors.initials: BD
authors.lastname: Bueltmann
mesh_terms: D006801:Humans; D010232:Paraffin; D046888:Tissue Array Analysis / Q000191:economics / Q000295:instrumentation / Q000379:methods*
chemical_list: D010232:Paraffin