[The research of molecular manifestations of genome instability for individuals exposed to ionizing radiation in clinical significant doses].
The oxidative status (effective contents of a superoxide anion-radical (O2*-) in assay with methyltetrazolium compound MTT and the contents of the reactive oxygen species (ROS) in assay with dichlorodihydrofluorescein diacetate (DCF-DA), the state of DNA structure and the nature of correlation connection between these indexes in cells of a peripheral blood for 26 healthy donors and 26 patients (including exposed in emergency on Chernobyl power plant), examined in many years after the radiation exposure in clinically significant doses are investigated. The method of polymerase chain reaction-single strand conformation polymorphism in the same cells out the search of mutation changes in 5-8 exons of TP53 gene for 10 patients was carried. It was established, that the state of the oxidative status for the patients is characterized by lowered (in relation to the donor control of equal age) contents O2*- in a general fraction of leucocytes and in mononuclear cells accordingly on 25% (p < 0.001) and 30% (p < 0.003) and ROS in mononuclear more, than on 40% (p < 0.008). During 5-hour incubation of cells the positive correlation connection (unidirectional changes) between indexes of DNA nativity and of oxidative status of leucocytes for 36.4% of the donors and 13.3% of the patients and in mononuclears for 38.5% of the donors and for 20% of the patients (p < 0.05) was detected. The general part of the individuals with a direct correlation in any of above named cell fractions among the donors in 2 times was higher (61.5%), than among the patients (26.7%, p = 0.038). The mutation replacements in nucleotide sequences of 5-8 exones of TP53 genes for the inspected patients were not detected. The obtained data indicate the existence of such changes in a metabolism and in systems of its regulation in nuclear blood cells after irradiation, which can be conditioned or can mirror a state of radiation-induced genome instability. The presence for the majority of the patients on the moment of the examination of several chronic diseases does not eliminate the connection of the detected biomolecular changes with these pathologies.
D000328:Adult; D047852:Chernobyl Nuclear Accident; D004247:DNA / Q000528:radiation effects; D005091:Exons / Q000235:genetics; D042822:Genomic Instability; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016273:Occupational Exposure; D018384:Oxidative Stress; D011832:Radiation Injuries / Q000235:genetics*; D011839:Radiation, Ionizing; D013481:Superoxides / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D016159:Tumor Suppressor Protein p53; D013481:Superoxides; D004247:DNA