[Relationship between the injury time and DNA fragmentation and its quantity in rat brain].
OBJECTIVE
To observe the changes of DNA fragmentation and its quantity along with the time of injury in nerve cells after brain contusion.


METHODS
The model of brain contusion caused by free drop hammer was established. TUNEL and Feulgen's DNA staining conjoined with image analysis technique were used for exploration.


RESULTS
With the gradually rising of DNA fragmentation, DNA quantity was declining in the brain tissue after contusion.


CONCLUSIONS
TUNEL and Feulgen's DNA staining conjoined with image analysis technique could be utilized in the timing of brain injury and provide a new approach for this issue.
Li
Fan
F
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. fanlfy@mail.haust.edu.cn
Liao
Zhi-Gang
ZG
Ma
Shu-Ling
SL
Wang
Ye
Y
Guan
Peng
P
Liu
Min
M
D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology*; D004247:DNA / Q000032:analysis*; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D049429:Forensic Pathology; D020287:In Situ Nick-End Labeling / Q000379:methods*; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
D004247:DNA