Sennosides A and B production by hairy roots of Senna alata (L.) Roxb.
Hairy roots of Senna alata transformed with Agrobacterium rhizogenes, strain ATCC 15834 were induced and grown in half-strength Murashige and Skoog (MS) medium. Effects of sucrose contents and hormones on the growth and sennosides A, B production were investigated. Hairy roots cultured on hormone-free half-strength MS medium containing 5% sucrose under dark condition mostly stimulated the growth of hairy roots and increased the content of sennosides A and B yielding (169 +/- 4) and (34 +/- 3) microg g(-1) dry wt, respectively.
Putalun
Waraporn
W
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. waraporn@kku.ac.th
Pimmeuangkao
Suwat
S
De-Eknamkul
Wanchai
W
Tanaka
Hiroyuki
H
Shoyama
Yukihiro
Y
D000880:Anthraquinones / Q000378:metabolism*; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000502:physiology; D036226:Seedlings / Q000378:metabolism / Q000382:microbiology; D012639:Seeds; D012676:Senna Extract; D029846:Senna Plant / Q000378:metabolism*; D000081226:Sennosides; D012709:Serum Albumin
D000880:Anthraquinones; D017931:DNA Primers; D004273:DNA, Neoplasm; D000081226:Sennosides; D012709:Serum Albumin; D012676:Senna Extract