title: Mutations and promoter methylation status of NPM1 in myeloproliferative disorders.
abstract: We determined mutations and promoter methylation status of NPM1 using pyrosequencing in 199 samples of myeloid neoplasia including myeloproliferative disorders (MPD). The mutations were present in 4% of chronic myelomonocytic leukemia, but not in other MPD or myelodysplastic syndromes. Promoter methylation was rare, and was found in only three samples of MPD.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Oki
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Jelinek
authors.forename: Miloslav
authors.initials: M
authors.lastname: Beran
authors.forename: Srdan
authors.initials: S
authors.lastname: Verstovsek
authors.forename: Hagop M
authors.initials: HM
authors.lastname: Kantarjian
authors.forename: Jean-Pierre J
authors.initials: JP
authors.lastname: Issa
mesh_terms: D001483:Base Sequence; D019175:DNA Methylation; D017931:DNA Primers; D014644:Genetic Variation; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics; D009154:Mutation; D009196:Myeloproliferative Disorders / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D000090243:Nucleophosmin; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic
chemical_list: D017931:DNA Primers; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D000090243:Nucleophosmin