title: Electrophoretic and mass spectrometric strategies for profiling bacterial lipopolysaccharides.
abstract: Capillary electrophoresis (CE) is a high-resolution separation technique that has been widely used for trace analysis in biological samples. On-line capillary electrophoresis-electrospray mass spectrometry (CE-MS) was developed for the analysis of lipopolysaccharide (LPS) glycoforms from the gram-negative bacteria, Haemophilus influenzae. In this paper, we report on the application of CE-MS to characterize structural differences in O-deacylated LPS samples from H. influenzae strains Rd 11.7 and 375.1. The resolution capability of on-line CE-MS was first demonstrated by analysis of a complex LPS mixture from H. influenzae strain Rd 11.7. This strain contains a mixture of isomeric glycoforms differing in the number and positions of hexose moieties. Sialic acid containing glycoforms were also determined. Structural features of LPS from a lic1 mutant of H. influenzae strain 375 (375.1) were studied using on-line CE-MS/MS. With the separation provided by CE, two isomeric glycoforms differing in the location of phosphoethanolamine substituents were characterized by tandem mass spectrometry.
authors.affiliation: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada K1A 0R6. Jianjun.Li@nrc-cnrc.gc.ca
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Cox
authors.forename: Derek W
authors.initials: DW
authors.lastname: Hood
authors.forename: Elke K H
authors.initials: EK
authors.lastname: Schweda
authors.forename: E Richard
authors.initials: ER
authors.lastname: Moxon
authors.forename: James C
authors.initials: JC
authors.lastname: Richards
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods; D006193:Haemophilus influenzae / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000032:analysis*; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008070:Lipopolysaccharides; D019158:N-Acetylneuraminic Acid