title: Acetylcholinesterase activity in rats experimentally demyelinated with ethidium bromide and treated with interferon beta.
abstract: The ethidium bromide (EB) demyelinating model was associated with interferon beta (IFN-beta) to evaluate acetylcholinesterase (AChE) activity in the striatum (ST), hippocampus (HP), cerebral cortex (CC), cerebellum (CB), hypothalamus (HY), pons (PN) and synaptosomes from the CC. Rats were divided into four groups: I control (saline), II (IFN-beta), III (EB) and IV (EB and IFN-beta). After 7, 15 and 30 days rats (n = 6) were sacrificed, and the brain structures were removed for enzymatic assay. AChE activity was found to vary in all the brain structures in accordance with the day studied (7-15-30 days) (P < 0.05). In the group III, there was an inhibition of the AChE activity in the ST, CB, HY, HP and also in synaptosomes of the CC (P < 0.05). It was observed that IFN-beta per se was capable to significantly inhibit (P < 0.05) AChE activity in the ST, HP, HY and synaptosomes of the CC. Our results suggest that one of the mechanisms of action of IFN-beta is through the inhibition of AChE activity, and EB could be considered an inhibitor of AChE activity by interfering with cholinergic neurotransmission in the different brain regions.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcellos, 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil.
authors.forename: C M
authors.initials: CM
authors.lastname: Mazzanti
authors.forename: R M
authors.initials: RM
authors.lastname: Spanevello
authors.forename: L B
authors.initials: LB
authors.lastname: Pereira
authors.forename: J F
authors.initials: JF
authors.lastname: Gonçalves
authors.forename: R
authors.initials: R
authors.lastname: Kaizer
authors.forename: M
authors.initials: M
authors.lastname: Corrêa
authors.forename: M
authors.initials: M
authors.lastname: Ahmed
authors.forename: A
authors.initials: A
authors.lastname: Mazzanti
authors.forename: R
authors.initials: R
authors.lastname: Festugatto
authors.forename: D L
authors.initials: DL
authors.lastname: Graça
authors.forename: V M
authors.initials: VM
authors.lastname: Morsch
authors.forename: M R C
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology*; D002800:Cholinesterase Inhibitors / Q000378:metabolism; D003711:Demyelinating Diseases / Q000139:chemically induced*; D004996:Ethidium / Q000633:toxicity*; D016899:Interferon-beta / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000201:enzymology
chemical_list: D002800:Cholinesterase Inhibitors; D016899:Interferon-beta; D000110:Acetylcholinesterase; D004996:Ethidium