[The influence of glucocorticoid immunosuppression on the course of experimental influenza pneumonia].
The study demonstrates the effects of kenalog (Kn), a synthetic glucocorticoid hormone, on the course of virus A/Aichi/2/68 influenza in white mice. In doses of 5 and 10 mg/kg, Kn reduced the weight of the adrenal glands, thymus and spleen, which was accompanied by decrease of the resistance to the mentioned virus, judging by LD50 decrease vs. this index in the control infected group. Besides, four days after infecting with 5 LD50 of influenza virus (IV), lung virus and interferon titers were significantly lower in mice pretreated with Kn vs. mice treated with placebo. Lung cell susceptibility to IV in vitro was identical in mice treated with Kn or placebo. In ultrathin lung sections of IV-infected mice, both experimental and control ones, there was virus budding in bronchial epithelium cells and type I and II alveolocytes. Analysis of inflammatory effusion compound in semithin lung sections 6 days after IV infection, found a substantially smaller number of mature alveolar macrophages (AM) and a bigger number of neutrophiles vs. infected controls. The authors reckon that higher mortality of mice pretreated with Kn before infecting, is caused not by enhancement of IV reproduction in target lung cells during influenza development, but by the contribution of other pathogenic factors. One of those may be increase of neutrophilic migration into the lungs; neutrophiles are more able to realize their significant destructive potential under the condition of reduction in the clearing function of AM and IV infection.
Smetannikova
M A
MA
Shishkina
L N
LN
Sergeev
A A
AA
Fankin
I V
IV
Bulychev
L G
LG
Kolesnikova
L V
LV
Omigov
V V
VV
Malkova
E M
EM
Petrishchenko
V A
VA
Sergeev
A N
AN
Zhukov
V A
VA
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use*; D007165:Immunosuppression Therapy / Q000379:methods*; D009980:Influenza A virus / Q000302:isolation & purification*; D008297:Male; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000188:drug therapy* / Q000276:immunology; D011024:Pneumonia, Viral / Q000188:drug therapy* / Q000276:immunology; D016896:Treatment Outcome
D005938:Glucocorticoids