title: [A comparative study of the thermal stability of formate dehydrogenases from microorganisms and plants].
abstract: A comparative study of the thermostability of NAD+-dependent formate dehydrogenases (FDHs; EC 1.2.1.2) from both methylotrophic bacteria Pseudomonas sp. 101 and Moraxella sp. Cl, the methane-utilizing yeast Candida boidinii, and plants Arabidopsis thaliana and Glycine max (soybean) was performed. All the enzymes studied were produced by expression in E. coli cells. The enzymes were irreversibly inactivated in one stage according to first-order reaction kinetics. The FDH from Pseudomonas sp. 101 appeared as the most thermostable enzyme; its counterpart from G. max exhibited the lowest stability. The enzymes from Moraxella sp. Cl, C. boidinii, and A. thaliana showed similar thermostability profiles. The temperature dependence of the inactivation rate constant of A. thaliana FDH was studied. The data of differential scanning calorimetry was complied with the experimental results on the inactivation kinetics of these enzymes. Values of the melting heat were determined for all the enzymes studied.
authors.forename: E G
authors.initials: EG
authors.lastname: Sadykhov
authors.forename: A E
authors.initials: AE
authors.lastname: Serov
authors.forename: N S
authors.initials: NS
authors.lastname: Voĭnova
authors.forename: S V
authors.initials: SV
authors.lastname: Uglanova
authors.forename: A S
authors.initials: AS
authors.lastname: Petrov
authors.forename: A A
authors.initials: AA
authors.lastname: Alekseeva
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Kleĭmenov
authors.forename: V I
authors.initials: VI
authors.lastname: Popov
authors.forename: V I
authors.initials: VI
authors.lastname: Tishkov
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D002175:Candida / Q000201:enzymology* / Q000235:genetics; D005560:Formate Dehydrogenases / Q000737:chemistry* / Q000235:genetics; D006358:Hot Temperature; D007700:Kinetics; D009016:Moraxella / Q000201:enzymology* / Q000235:genetics; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics; D013045:Species Specificity
chemical_list: D011994:Recombinant Proteins; D005560:Formate Dehydrogenases