title: Analysis of the periodontal microbiota in childhood-type hypophosphatasia.
abstract: Seven patients with childhood-type hypophosphatasia and healthy control subjects were analysed for microbiological and clinical evidence of periodontitis. The bacterial composition of the subgingival plaque was analysed by 16S rRNA gene cloning and sequencing, DNA-DNA hybridisation with specific probes, and specific PCR. With the exception of the genus Veillonella and the species Eikenella corrodens, no differences between the cohorts in the composition of bacterial orders, families, genera, and species were observed. Accordingly, patients showed only slightly more bleeding on probing (BOP), and slightly higher periodontal probing depth (PPD) values than controls. Although this pilot study did not have the power to assess the prevalence of periodontitis in HP, we suggest that most paediatric patients suffering from HP harbour a subgingival microbiota, which is comparable to that of the healthy population.
authors.affiliation: Institute for Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Strasse 2/E1, D-97080 Würzburg, Germany.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Valenza
authors.forename: Stefan
authors.initials: S
authors.lastname: Burgemeister
authors.forename: Hermann
authors.initials: H
authors.lastname: Girschick
authors.forename: Christoph
authors.initials: C
authors.lastname: Schoen
authors.forename: Simone
authors.initials: S
authors.lastname: Veihelmann
authors.forename: Annette
authors.initials: A
authors.lastname: Moter
authors.forename: Vesna
authors.initials: V
authors.lastname: Haban
authors.forename: Ulrich
authors.initials: U
authors.lastname: Vogel
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schlagenhauf
mesh_terms: D000293:Adolescent; D000328:Adult; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D015331:Cohort Studies; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D020459:Genes, rRNA / Q000235:genetics; D006801:Humans; D007014:Hypophosphatasia / Q000150:complications*; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000473:pathology; D010518:Periodontitis / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S