Systemic and mucosal immunity induced by oral somatic transgene vaccination against glycoprotein B of pseudorabies virus using live attenuated Salmonella typhimurium.
Glycoprotein B mediates the absorption and penetration of the pseudorabies virus in the form of an immunodominant Ag, and represents a major target for the development of new vaccines. This study evaluated the efficiency of live attenuated Salmonella typhimurium SL7207 for the oral delivery of DNA vaccine encoding the pseudorabies virus glycoprotein B (pCI-PrVgB) in vivo, leading to the generation of both systemic and mucosal immunity against the pseudorabies virus Ag. An oral transgene vaccination of pCI-PrVgB using a Salmonella carrier produced a broad spectrum of immunity at both the systemic and mucosal sites, whereas the intramuscular administration of a naked DNA vaccine elicited no mucosal immunoglobulin (Ig)A response. Interestingly, the Salmonella-mediated oral transgene vaccination of the pseudorabies virus glycoprotein B biased the immune responses to the Th2-type, as determined by the IgG2a/IgG1 ratio and the cytokine production profile. However, oral vaccination mediated by Salmonella harbouring pCI-PrVgB showed inferior protection to systemic immunization against virulent pseudorabies virus infection. The expression of transgene delivered by Salmonella bacteria in antigen-presenting cells of both the systemic and mucosal-associated lymphoid tissues was further demonstrated. These results highlight the potential use of live attenuated S. typhimurium for an oral transgene pseudorabies virus glycoprotein B vaccination to induce broad immune responses.
Eo
Seong-Kug
SK
Department of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju, Korea. vetvirus@chonbukakcr
Yoon
Hyun-A
HA
Aleyas
Abi George
AG
Park
Seong-Ok
SO
Han
Young-Woo
YW
Chae
Joon-Seok
JS
Lee
John-Hwa
JH
Song
Hee-Jong
HJ
Cho
Jeong-Gon
JG
D000284:Administration, Oral; D000818:Animals; D005260:Female; D018928:Immunity, Mucosal; D051379:Mice; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control; D022421:Pseudorabies Vaccines / Q000008:administration & dosage* / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology*; D014611:Vaccination; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D022421:Pseudorabies Vaccines; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C070915:glycoprotein gII, pseudorabies virus