[The chemotaxis effect of ampelopsin on the immunocytes].
OBJECTIVE
To study the chemotaxis effect of ampelopsin with different concentration on monocytes and neutrophilic granulocytes.


METHODS
Chemokinesis and chemotaxis tests were proceed in agarose gel comparing with chemokine IL-8 or MCP-1.


RESULTS
At 25.6 microg/ml or 51.2 microg/ml, ampelopsin could strongly enhance the migration of neutrophilic granulocytes and monocytes. The chemotaxis effect induced by 25.6 microg/ml of ampelopsin had no significant differences with that induced by 150 ng/ml of IL-8 or 50 ng/ml of MCP-1 (P > 0.05). At a concentration of 12.8 microg/ml, the chemokime effect of ampelopsin was more potent than that of 150 ng/ml of IL-8 or 100 ng/ml of MCP-1 (P < 0.05). Ampelopsin exerted a synergistic action with IL-8 or MCP-1 on its chemotaxis effect to neutrophilic granulocytes and monocytes.


CONCLUSIONS
Ampelopsin can strongly enhance the chemokinesis and chemotaxis effects of neutrophilic granulocytes and moncytes and exert a synergistic action with IL-8 or MCP-1 on its chemotaxis effect to neutrophilic granulocytes and monocytes.
Zeng
Sa
S
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510080, China.
Luo
Gao-Qin
GQ
Liu
De-Yu
DY
D032421:Ampelopsis / Q000737:chemistry*; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D016209:Interleukin-8 / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D010946:Plants, Medicinal / Q000737:chemistry; D012685:Sepharose
D018932:Chemokine CCL2; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D016209:Interleukin-8; C106407:ampelopsin; D012685:Sepharose