[Cellular mechanism of heart injury in the early stage of crush injury in rats].
OBJECTIVE
To study cellular mechanism of cardiomyocytes injury in the early stage of crush injury by observing some effects of crush injury rat sera on cultured neonatal rat cardiomyocytes.


METHODS
One to three days old neonatal rat cardiomyocytes were cultured in vitro and some effects of crush injury rat sera on beating rate, cell surface area, total protein content, 3H-Leu incorporation, intracellular calcium concentration ([Ca2+]i) and Fos protein expression were observed in cultured rat cardiomyocytes.


RESULTS
Compared with normal rat serum group, crush injury rat sera decreased beating rate(beats/min) of cardiomyocytes from 88.3 to 26.4, cell surface area, total protein content, 3H-Leu incorporation, [Ca2+]i (nmol/L) and PI of Fos protein expression were increased.


CONCLUSIONS
Crush injury rat sera suppress cell beating, increase intracellular calcium, induce Fos protein synthesis and cause cell hypertrophy, which may cause cardiac injury in the early stage of rush injury.
Liu
Shui-Ping
SP
Department of Forensic Pathology, Preclinical School of Sun Yat-sen University, Guangzhou 510080, China.
Liu
Xiao-Shan
XS
Jing
Hua-Lan
HL
Li
Zhao-Hui
ZH
Chen
Yu-Chuan
YC
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005121:Extremities / Q000293:injuries; D006335:Heart Injuries / Q000378:metabolism / Q000473:pathology*; D006339:Heart Rate / Q000187:drug effects; D007106:Immune Sera / Q000494:pharmacology*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007106:Immune Sera; D016760:Proto-Oncogene Proteins c-fos; D002118:Calcium