Mutational analysis of MYC in common epithelial cancers and acute leukemias.
In addition to chromosomal translocation, the MYC gene in the N-terminal coding region showed clustered point mutations in Burkitt's lymphomas. A recent study showed that these point mutations inactivated the apoptosis activity of MYC and contributed to the tumor transformation. To see whether the point mutations of MYC are involved in tumors besides Burkitt's lymphomas, we analyzed the N-terminal cluster region of the mutations in 507 cancers from gastric, colorectal, breast and lung carcinomas, and acute leukemias, by polymerase chain reaction-based single-strand conformation polymorphism assay. However, there was no somatic mutation of the MYC gene in the cancers. The data suggest that the point mutation of the MYC gene in the N-terminal domain may be very rare and may not contribute to the development of common human cancers besides Burkitt's lymphomas.
Lee
Jong Woo
JW
Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Soung
Young Hwa
YH
Kim
Su Young
SY
Nam
Suk Woo
SW
Park
Won Sang
WS
Lee
Jung Young
JY
Yoo
Nam Jin
NJ
Lee
Sug Hyung
SH
D000208:Acute Disease; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D005260:Female; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D004273:DNA, Neoplasm