Phylogenetic analysis of Nosema antheraeae (Microsporidia) isolated from Chinese oak silkworm, Antheraea pernyi.
The microsporidian Nosema antheraeae is a pathogen that infects the Chinese oak silkworm, Antheraea pernyi. We sequenced the complete small subunit (SSU) rRNA gene and the internal transcribed spacer (ITS) of N. antheraeae, and compared the SSU rRNA sequences in other microsporidia. The results indicated that Nosema species, including N. antheraeae, formed two distinct clades, consistent with previous observations. Furthermore, N. antheraeae is clustered with N. bombycis with high bootstrap support. The organization of the rRNA gene of N. antheraeae is LSU-ITS1-SSU-ITS2-5S, also following a pattern similar to the Nosema type species, N. bombycis. Thus, N. antheraeae is a Nosema species and has a close relationship to N. bombycis.
Wang
Lin-Ling
LL
Institute of Life Sciences, The University of JiangSu, ZhenJiang 212013, China.
Chen
Ke-Ping
KP
Zhang
Ze
Z
Yao
Qin
Q
Gao
Gui-Tian
GT
Zhao
Yuan
Y
D000818:Animals; D012831:Bombyx / Q000382:microbiology*; D002681:China; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005800:Genes, Fungal; D020459:Genes, rRNA; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D016818:Nosema / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics*
D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal