title: The control of UCP1 is dissociated from that of PGC-1alpha or of mitochondriogenesis as revealed by a study using beta-less mouse brown adipocytes in culture.
abstract: In rodent brown adipose tissue, the beta-adrenergic signaling is believed, by an action on PGC-1alpha, to control UCP1 expression and mitochondriogenesis. We addressed this hypothesis using beta(1)/beta(2)/beta(3)-adrenoceptor knockout (beta-less) brown adipocytes in primary culture. In these cells: (a) proliferation and differentiation into multilocular cells were normal; (b) UCP1 mRNA expression was dramatically decreased (by 93%), whereas PGC-1alpha and mtTFA mRNA expressions were not; (c) UCP1, PGC-1alpha and COX IV protein expressions were decreased by 97%, 62% and 22%, respectively. Altogether the data show a dissociation between the control of UCP1, which is mostly beta-adrenoceptor-dependent and that of PGC-1alpha and of mitochondriogenesis which are not.
authors.affiliation: Department of Cell Physiology and Metabolism, Centre Médical Universitaire, Geneva, Switzerland. lehr6@etu.unige.ch
authors.forename: Lorenz
authors.initials: L
authors.lastname: Lehr
authors.forename: Kriss
authors.initials: K
authors.lastname: Canola
authors.forename: Cedric
authors.initials: C
authors.lastname: Asensio
authors.forename: Maria
authors.initials: M
authors.lastname: Jimenez
authors.forename: Françoise
authors.initials: F
authors.lastname: Kuehne
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Giacobino
authors.forename: Patrick
authors.initials: P
authors.lastname: Muzzin
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers; D007473:Ion Channels; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D024101:Mitochondrial Proteins; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D011943:Receptors, Adrenergic, beta / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000502:physiology*; D014157:Transcription Factors; D000071256:Uncoupling Protein 1
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D015534:Trans-Activators; D014157:Transcription Factors; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1