title: Aovps24, a homologue of VPS24, is required for vacuolar formation which could maintain proper growth and development in the filamentous fungus Aspergillus oryzae.
abstract: Vps24 (vacuolar protein sorting) is a component of ESCRT III (endosomal sorting complex required for transport), which is required for the formation of MVB (multivesicular body). We have isolated the VPS24 homologue gene, Aovps24, from the filamentous fungus Aspergillus oryzae, and analyzed the localization of AoVps24 using EGFP. AoVps24 was localized in the cytoplasm and late endosome-like structures. Furthermore, we constructed an Aovps24 disruptant, which showed impaired growth, conidiation, and hyphal morphology. In addition, normal vacuoles were not observed in the Aovps24 disruptant. In the Saccharomyces cerevisiae vps24 disruptant, the normal vacuoles are formed and it does not show the impaired growth and abnormal cell shape as the A. oryzae Aovps24 disruptant. The results suggest that AoVps24 is required for vacuolar formation and normal vacuoles could have the function to maintain the normal hyphal elongation and conidiation in A. oryzae.
authors.affiliation: Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Tatsumi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kikuma
authors.forename: Manabu
authors.initials: M
authors.lastname: Arioka
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kitamoto
mesh_terms: D001236:Aspergillus oryzae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005800:Genes, Fungal / Q000235:genetics; D014617:Vacuoles / Q000502:physiology*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D033921:Vesicular Transport Proteins