Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos.
Cytokinesis is the final stage in cell division that serves to partition cytoplasm and daughter nuclei into separate cells. Membrane remodeling at the cleavage plane is a required feature of cytokinesis in many species. In animal cells, however, the precise mechanisms and molecular interactions that mediate this process are not yet fully understood. Using real-time imaging in live, early stage zebrafish embryos, we demonstrate that vesicles labeled with the v-SNARE, VAMP-2, are recruited to the cleavage furrow during deepening in a microtubule-dependent manner. These vesicles then fuse with, and transfer their VAMP-2 fluorescent label to, the plasma membrane during both furrow deepening and subsequent apposition. This observation indicates that new membrane is being inserted during these stages of cytokinesis. Inhibition of SNAP-25 (a cognate t-SNARE of VAMP-2), using a monoclonal antibody, blocked VAMP-2 vesicle fusion and furrow apposition. Transient expression of mutant forms of SNAP-25 also produced defects in furrow apposition. SNAP-25 inhibition by either method, however, did not have any significant effect on furrow deepening. Thus, our data clearly indicate that VAMP-2 and SNAP-25 play an essential role in daughter blastomere apposition, possibly via the delivery of components that promote the cell-to-cell adhesion required for the successful completion of cytokinesis. Our results also support the idea that new membrane addition, which occurs during late stage cytokinesis, is not required for furrow deepening that results from contractile band constriction.
Li
Wai Ming
WM
Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, PRC.
Webb
Sarah E
SE
Lee
Karen W
KW
Miller
Andrew L
AL
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D048749:Cytokinesis / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000378:metabolism; D005089:Exocytosis; D049452:Green Fluorescent Proteins / Q000235:genetics; D008870:Microtubules / Q000378:metabolism; D050825:Synaptosomal-Associated Protein 25 / Q000502:physiology*; D050988:Vesicle-Associated Membrane Protein 2 / Q000502:physiology*; D015027:Zebrafish / Q000196:embryology
D050825:Synaptosomal-Associated Protein 25; D050988:Vesicle-Associated Membrane Protein 2; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins