In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles.
Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular replacement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications. Here, E14 mouse ES cells have been induced into hepatocytes in vitro. Morphology revealed that ES-derived hepatic-like cells were round or polyhedral shaped with distinct boundary of individual cells, and some arranged in trabeculae. These cells expressed endodermal- or liver-specific mRNA--transthyretin (TTR), alpha1-anti-trypsin (AAT), alpha-fetoprotein (AFP), albumin (ALB), glucose-6-phoshpatase (G6P) and tyrosine aminotransferase (TAT). Approximately (85.1 +/- 0.5)% of the ES-derived cells was stained positive green with ICG uptake. These cells were also stained magenta as a result of PAS reaction. In this paper, expression of coagulation factors VIII and IX mRNA in the ES-derived cells is documented. Therefore, ES cells might be developed as substitute donor cells for the therapy of coagulation factor deficiencies.
Meng
Ying
Y
Center for Stem Cell Research, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China. yingmeng2002@hotmail.com
Huang
Shaoliang
S
Min
Jun
J
Guo
Zhongmin
Z
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D017931:DNA Primers / Q000235:genetics; D004622:Embryo, Mammalian / Q000166:cytology*; D005164:Factor IX / Q000235:genetics*; D005169:Factor VIII / Q000235:genetics*; D020869:Gene Expression Profiling; D006467:Hemophilia A / Q000628:therapy; D002836:Hemophilia B / Q000628:therapy; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation
D017931:DNA Primers; D012333:RNA, Messenger; D005169:Factor VIII; D005164:Factor IX