Isolation and partial characterization of a novel porcine astrovirus.
Astroviral infection has been described as one of the causes of porcine diarrhoeal disease. Here we describe the detection of astrovirus-like particles by electron microscopy in a diarrhoeal specimen. Furthermore, a cytopathic virus was isolated and propagated in an established porcine kidney cell line, PK-15. Reverse transcription and PCR performed with astrovirus-specific primers amplified a product with the expected size. Sequencing of the PCR product revealed that the virus observed by electron microscopy and propagated in the porcine cell line is an astrovirus, showing 86% identity at the nucleotide level with the only known porcine astrovirus, PAstV. Phylogenetic analysis clustered the novel isolate, Sb4685, together with PAstV in a broad clade comprising mammalian astroviruses.
Indik
Stanislav
S
Research Institute of Virology and Biomedicine, University of Veterinary Medicine, Veterinärplatz 1, Vienna, Austria. stanislav.indik@vu-wien.ac.at
Valícek
Lubomír
L
Smíd
Bedrich
B
Dvoráková
Hana
H
Rodák
Ladislav
L
D000818:Animals; D019200:Astroviridae / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D019350:Astroviridae Infections / Q000662:veterinary*; D001483:Base Sequence; D002460:Cell Line; D016000:Cluster Analysis; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D005784:Gene Amplification; D008854:Microscopy, Electron / Q000662:veterinary; D008970:Molecular Weight; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D012367:RNA, Viral