Transformation of ciprofloxacin-resistant Neisseria gonorrhoeae gyrA, parE and porB1b genes.
In several transformation experiments, we have shown that introduction of an alteration in GyrA at position 95 of a ciprofloxacin-susceptible Neisseria gonorrhoeae strain (minimum inhibitory concentration (MIC) 0.008 mg/L) increases the MIC to 0.064 mg/L. Two alterations (positions 91 and 95) increase the MIC to 0.125-0.25 mg/L. Transformants with ciprofloxacin MICs of 0.5-16 mg/L were obtained from a moderately ciprofloxacin-resistant strain (MIC 0.25 mg/L). These transformants had alterations in the gene for PorB1b and probably other genes. In one transformant, an alteration in ParE was also introduced, which probably contributed to ciprofloxacin resistance. The ciprofloxacin-resistant transformants had the donor porB1b sequence, and most of them also had altered serovars. We conclude that alterations in N. gonorrhoeae PorB1b could be involved in ciprofloxacin resistance.
Lindbäck
Emma
E
Division of Clinical Bacteriology, Department of Laboratory Medicine, Karolinska Institute, F82 Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden. emma.lindback@capio.se
Islam
Sohidul
S
Unemo
Magnus
M
Lang
Camilla
C
Wretlind
Bengt
B
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D018272:Porins / Q000235:genetics; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D018272:Porins; C057699:porin protein, Neisseria; D002939:Ciprofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase