Separation, quantification and identification of non-volatile organic acids in body fluids by gas chromatography.
The present paper concentrates on the analysis of non-volatile organic acids in physiological fluids. The solvent extraction and DEAE-Sephadex extraction of organic acids, respectively, seem to be the most widely used methods for isolation of this group of compounds from the biological matrix. Gas chromatography-mass spectrometry (GC/MS) is the preferred method for the separation, quantification and identification. In three tables organic acids are divided into the classes according to a functional group, a survey of alkylation methods is given and retention indices of common urinary organic acids on various GC columns are summarized.
Verner
P
P
Faculty of General Medicine of the Charles' University, Diagnostic Centre for Congenital Metabolic Disorders, 121 11 Praha 2, Karlovo nám. 32, Czechoslovakia.