Effect of [Ca2+]i and neuronal mitochondria transmembrane potentials in hippocampus of murine cytomegalovirus infected mice.
To explore the effect of [Ca2+]i and neuronal mitochondria transmembrane potentials in hippocampus of murine cytomegalovirus (MCMV) infected mice, newborn Balb/c mice were randomly divided into two groups: a virus inoculated group and a control group. After 56 days, single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the intracellular free calcium level [Ca2+]i in hippocampus were measured by means of flow cytometry (FCM). Compared with the control group, the mitochondria transmembrane potentials was decreased (P<0.01) and the intracellular free calcium level [Ca2+]i was increased (P<0.01) in inoculated group. The dysfunction of [Ca2+]i and mitochondria transmembrane potentials in hippocampus may play an important role in the functional disorders in CMV-infected CNS.
Zhang
Ying
Y
Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Wen
Liangzhen
L
Cheng
Biheng
B
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D005260:Female; D006566:Herpesviridae Infections / Q000378:metabolism / Q000503:physiopathology*; D006624:Hippocampus / Q000378:metabolism / Q000503:physiopathology* / Q000821:virology; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018146:Muromegalovirus / Q000502:physiology; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D011897:Random Allocation
D002118:Calcium