Evaluation of c-MYC status in primary acquired cholesteatoma by using fluorescence in situ hybridization technique.
OBJECTIVE
The object of study was to investigate the status of c-MYC oncogene in primary acquired cholesteatoma.


METHODS
Descriptive study.


METHODS
Cholesteatoma samples were obtained from 15 patients with primary acquired cholesteatoma during surgical operation. Fluorescence in situ hybridization with a mixed DNA probe, which is specific for c-MYC located on 8q24 and chromosome 8 specific-alpha-satellite DNA probe (dual color), was used on the interphase nuclei.


RESULTS
Copy number of c-MYC oncogene and aneuploidy of chromosome 8 were 21.2% +/- 14.4% and 21.7% +/- 14.8%, respectively. There was no significant difference between copy number of c-MYC and frequency of chromosome 8 aneuploidy (p > 0.05). Ten of 15 cases showed different percentage of c-MYC and chromosome 8 aneuploidy, whereas 5 (33.3%) of 15 cases showed a normal distribution of c-MYC and chromosome 8 signals.


CONCLUSIONS
The copy number of c-MYC in 10 of 15 cases was found to be high as observed for chromosome 8 aneuploidy in primary acquired cholesteatoma. These findings suggest that the ability of hyperproliferation of primary acquired cholesteatoma might have been related to c-MYC copy number by deregulating c-MYC expression.
Ozturk
Kayhan
K
Department of Otolaryngology, Meram Medical Faculty, Selçuk University, Konya, Turkey. kayhanozturk@selcuk.edu.tr
Yildirim
Mahmut Selman
MS
Acar
Hasan
H
Cenik
Ziya
Z
Keles
Bahar
B
D000293:Adolescent; D000328:Adult; D000782:Aneuploidy; D018424:Cholesteatoma, Middle Ear / Q000235:genetics* / Q000473:pathology; D002898:Chromosomes, Human, Pair 8; D004268:DNA-Binding Proteins / Q000032:analysis*; D005260:Female; D016259:Genes, myc; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008297:Male; D008875:Middle Aged; D014157:Transcription Factors / Q000032:analysis*
D004268:DNA-Binding Proteins; C116115:MYCBP protein, human; D014157:Transcription Factors