Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells.
Amphiphilic macromolecules containing a polystyrene-adherent peptide domain and a cell-repellent poly(ethylene glycol) domain were designed, synthesized, and evaluated as a cytophobic surface coating. Such cytophobic, or cell-repellent, coatings are of interest for varied medical and biotechnological applications. The composition of the polystyrene binding peptide domain was identified using an M13 phage display library. ELISA and atomic force spectroscopy were used to evaluate the binding affinity of the amphiphile peptide domain to polystyrene. When coated onto polystyrene, the amphiphile reduced cell adhesion of two distinct mammalian cell lines and pathogenic Staphylococcus aureus strains.
Kenan
Daniel J
DJ
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA. kenan001@mc.duke.edu
Walsh
Elisabeth B
EB
Meyers
Steven R
SR
O'Toole
George A
GA
Carruthers
Erin G
EG
Lee
Woo K
WK
Zauscher
Stefan
S
Prata
Carla A H
CA
Grinstaff
Mark W
MW
D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000502:physiology
D010455:Peptides; D011092:Polyethylene Glycols