Cross-species overgo hybridization and comparative physical mapping within avian genomes.
The chicken genome sequence facilitates comparative genomics within other avian species. We performed cross-species hybridizations using overgo probes designed from chicken genomic and zebra finch expressed sequence tags (ESTs) to turkey and zebra finch BAC libraries. As a result, 3772 turkey BACs were assigned to 336 markers or genes, and 1662 zebra finch BACs were assigned to 164 genes. As expected, cross-hybridization was more successful with overgos within coding sequences than within untranslated region, intron or flanking sequences and between chicken and turkey, when compared with chicken-zebra finch or zebra finch-turkey cross-hybridization. These data contribute to the comparative alignment of avian genome maps using a 'one sequence, multiple genomes' strategy.
Romanov
M N
MN
Genetics Division, Conservation and Research for Endangered Species, Zoological Society of San Diego, Arnold and Mabel Beckman Center for Conservation Research, 15600 San Pasqual Valley Road, Escondido, CA 92027-7000, USA.
Dodgson
J B
JB
D000818:Animals; D001717:Birds / Q000235:genetics*; D002645:Chickens / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial; D015342:DNA Probes; D020224:Expressed Sequence Tags; D046369:Finches / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D016678:Genome; D023281:Genomics / Q000379:methods; D006824:Hybridization, Genetic; D020161:Physical Chromosome Mapping / Q000379:methods*; D014422:Turkeys / Q000235:genetics
D015342:DNA Probes; D005819:Genetic Markers