title: Production of a metal-binding exopolysaccharide by Paenibacillus jamilae using two-phase olive-mill waste as fermentation substrate.
abstract: The present study investigated the use of two-phase olive mill waste (TPOMW) as substrate for the production of exopolysaccharide (EPS) by the endospore-forming bacilli Paenibacillus jamilae. This microorganism was able to grow and produce EPS in aqueous extracts of TPOMW as a unique source of carbon. The effects of substrate concentration and the addition of inorganic nutrients were investigated. Maximal polymer yield in 100-ml batch-culture experiments (2 g l(-1)) was obtained in cultures prepared with an aqueous extract of 20% TPOMW (w/v). An inhibitory effect was observed on growth and EPS production when TPOMW concentration was increased. Nutrient supplementation (nitrate, phosphate, and other inorganic nutrients) did not increase yield. Finally, an adsorption experiment of Pb (II), Cd (II), Cu (II), Zn (II), Co (II), and Ni (II) by EPS is reported. Lead was preferentially complexed by the polymer, with a maximal uptake of 230 mg/g EPS.
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, Granada 18071, Spain.
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Morillo
authors.forename: Margarita
authors.initials: M
authors.lastname: Aguilera
authors.forename: Alberto
authors.initials: A
authors.lastname: Ramos-Cormenzana
authors.forename: Mercedes
authors.initials: M
authors.lastname: Monteoliva-Sánchez
mesh_terms: D018533:Biomass; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D005285:Fermentation; D016937:Gram-Positive Endospore-Forming Rods / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D007220:Industrial Waste; D007700:Kinetics; D019216:Metals, Heavy / Q000737:chemistry / Q000378:metabolism; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007220:Industrial Waste; D019216:Metals, Heavy; D000069463:Olive Oil; D010938:Plant Oils; D011135:Polysaccharides, Bacterial