title: [Relationship between expression of nestin in experimental brain contusion and injury time].
abstract: OBJECTIVE
  To observe the alteration of nestin intervals in the experimental traumatic brain injury and investigate its relation to the injury intervals.
  
  
  METHODS
  The rat brain contusion was conducted by falling impact injury. After various survival interval (0.5, 6, 12 h and 1, 3, 7, 14, 28 d), immunohistochemical SP method was used for observing the expression of nestin in the cortex, hippocampal dentate gyrus and the corpus callosum on injury side.
  
  
  RESULTS
  Expression of nestin positive cells increased at 0.5 h and reached the maximum level in 7 d after brain contusion, then the expression decreased gradually. The intensity of nestin staining in the the cortex and the hippocampal dentate gyrus decreased to normal on 28 d. As to the corpus callosum of injury side it remained weak on 28 d.
  
  
  CONCLUSIONS
  The changes of nestin immunohistochemical staining can be used as an index for forensic estimation of early injury time.
authors.affiliation: Department of Forensic Pathology, Tongji Medical college, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Jia
authors.forename: Guang-Long
authors.initials: GL
authors.lastname: He
authors.forename: Yi-Wu
authors.initials: YW
authors.lastname: Zhou
authors.forename: Wei-Nian
authors.initials: WN
authors.lastname: Deng
authors.forename: Lai-Jing
authors.initials: LJ
authors.lastname: Sun
authors.forename: Jia-Pei
authors.initials: JP
authors.lastname: Dai
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.forename: Xiao-Hu
authors.initials: XH
authors.lastname: Xu
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin