The -56T HLA-G promoter polymorphism is not associated with pre-eclampsia/eclampsia in Australian and New Zealand women.
OBJECTIVE
Decreased HLA-G expression has been linked to a number of pregnancy disorders, including preeclampsia, and a genetic basis for HLA-G regulation has yet to be found. The aim of this study was to determine whether a C-to-T base substitution 56 base pairs (bp) upstream from the HLA-G transcription start site is associated with preeclampsia.


METHODS
277 nulliparous women consisting of 113 normotensive, 118 preeclamptic, and 46 eclamptic patients were typed for the -56T polymorphism using restriction fragment length polymorphism and allelic discrimination analysis.


RESULTS
-56T allele frequencies for eclamptic, preeclamptic, and normotensive women were 0.053, 0.030, and 0.035, respectively. A chi2 test indicated that there was no significant association with the polymorphism in preeclamptic or eclamptic women with p > 0.05.


CONCLUSIONS
The -56T HLA-G polymorphism is not associated with preeclampsia or eclampsia in our population.
Doherty
Vicki L
VL
Department of Perinatal Medicine, The Royal Women's Hospital, Carlton, Victoria, Australia.
Rush
Ashley N
AN
Brennecke
Shaun P
SP
Moses
Eric K
EK
D001315:Australia; D016022:Case-Control Studies; D004461:Eclampsia / Q000235:genetics*; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D009520:New Zealand; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D013941:Thymine
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D013941:Thymine