The role of Schizosaccharomyces pombe DNA repair enzymes Apn1p and Uve1p in the base excision repair of apurinic/apyrimidinic sites.
In Schizosaccharomyces pombe the repair of apurinic/apyrimidinic (AP) sites is mainly initiated by AP lyase activity of DNA glycosylase Nth1p. In contrast, the major AP endonuclease Apn2p functions by removing 3'-alpha,beta-unsaturated aldehyde ends induced by Nth1p, rather than by incising the AP sites. S. pombe possesses other minor AP endonuclease activities derived from Apn1p and Uve1p. In this study, we investigated the function of these two enzymes in base excision repair (BER) for methyl methanesulfonate (MMS) damage using the nth1 and apn2 mutants. Deletion of apn1 or uve1 from nth1Delta cells did not affect sensitivity to MMS. Exogenous expression of Apn1p failed to suppress the MMS sensitivity of nth1Delta cells. Although Apn1p and Uve1p incised the oligonucleotide containing an AP site analogue, these enzymes could not initiate repair of the AP sites in vivo. Despite this, expression of Apn1p partially restored the MMS sensitivity of apn2Delta cells, indicating that the enzyme functions as a 3'-phosphodiesterase to remove 3'-blocked ends. Localization of Apn1p in the nucleus and cytoplasm hints at an additional function of the enzyme other than nuclear DNA repair. Heterologous expression of Saccharomyces cerevisiae homologue of Apn1p completely restored the MMS resistance of the nth1Delta and apn2Delta cells. This result confirms a difference in the major pathway for processing the AP site between S. pombe and S. cerevisiae cells.
Tanihigashi
Haruna
H
Department of Biochemistry, Faculty of Science, Okayama University of Science, 1-1 Ridaicho, Okayama 700-0005, Japan.
Yamada
Ayako
A
Igawa
Emi
E
Ikeda
Shogo
S
D001761:Bleomycin / Q000494:pharmacology; D002467:Cell Nucleus / Q000201:enzymology; D004260:DNA Repair / Q000502:physiology*; D045643:DNA Repair Enzymes; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000378:metabolism / Q000502:physiology*; D004706:Endodeoxyribonucleases / Q000502:physiology*; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology; D012568:Schizosaccharomyces / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism / Q000502:physiology*
D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D001761:Bleomycin; C105427:Zeocin; D008741:Methyl Methanesulfonate; D004706:Endodeoxyribonucleases; C083274:Apn1 protein, S cerevisiae; C105073:uve1 protein, S pombe; C480730:Apn1 protein, S pombe; C480729:Apn2 protein, S pombe; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D045643:DNA Repair Enzymes