title: Methodology of reversible protein labeling for ratiometric fluorescent measurement.
abstract: The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique amino acid tag.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Soh
authors.forename: Daisuke
authors.initials: D
authors.lastname: Seto
authors.forename: Koji
authors.initials: K
authors.lastname: Nakano
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Imato
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D002021:Buffers; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008954:Models, Biological; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D000596:Amino Acids; D002021:Buffers; D005456:Fluorescent Dyes; D011506:Proteins