title: Calbindin D-28k immunoreactivity increases in the hippocampus after long-term treatment of soy isoflavones in middle-aged ovariectomized and male rats.
abstract: This article investigates the long-term effects of soybean isoflavones (ISO) on the changes of calbindin D-28k (CB) immunoreactivity in the hippocampus in middle-aged ovariectomized female rats as well as middle-aged control female and male rats to identify any correlation between calcium and phytoestrogens. In the CA1 region, CB immunoreactivity in the ovariectomized females was similar to that of the control females, whereas CB immunoreactivity in the males was significantly lower than that of the control females. In the dentate gyrus, CB immunoreactivity in the ovariectomized females and males was significantly lower than that of the control females. CB immunoreactivity in all groups was increased dose-dependently after ISO treatment in the CA1 region and dentate gyrus. This result suggests that ISO treatment enhances the expression of CB immunoreactivity in the hippocampus in the middle-aged rats.
authors.affiliation: Department of Anatomy College of Medicine, Hallym University, Chunchon, South Korea.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Yoon-Bok
authors.initials: YB
authors.lastname: Lee
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
authors.forename: Heon-Soo
authors.initials: HS
authors.lastname: Sohn
authors.forename: Sang Moo
authors.initials: SM
authors.lastname: Kim
authors.forename: Woo-Jung
authors.initials: WJ
authors.lastname: Kim
authors.forename: Hyun Kyung
authors.initials: HK
authors.lastname: Shin
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000367:Age Factors; D000818:Animals; D064026:Calbindins; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects*; D007150:Immunohistochemistry / Q000379:methods; D007529:Isoflavones / Q000008:administration & dosage*; D008297:Male; D010052:Ovariectomy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D030262:Soybean Proteins / Q000008:administration & dosage*
chemical_list: D064026:Calbindins; D007529:Isoflavones; D064030:S100 Calcium Binding Protein G; D030262:Soybean Proteins