Expression of ADP-ribosylation factor-like protein 8B mRNA in the brain is down-regulated in mice fed a high-fat diet.
A differential display was performed to analyze differential gene expression in the brains of mice in association with dietary high beef-tallow. Consumption of a high beef-tallow diet downregulated the expression of ADP-ribosylation factor-like protein 8B (Arl8B) mRNA in the brain. Arl8B mRNA was widely expressed in the mouse brain, including primary neuronal cells. The current study indicates that green fluorescent protein-fused Arl8B protein accumulated at the growth cones in primary neuronal cells, and that protrusions of human embryonic kidney 293 (HEK293) cells were significantly elongated by overexpression of Arl8B, suggesting an important role of Arl8B in neurite formation.
Haraguchi
Tomoaki
T
Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
Yanaka
Noriyuki
N
Nogusa
Yoshihito
Y
Sumiyoshi
Naoki
N
Eguchi
Yuko
Y
Kato
Norihisa
N
D020727:ADP-Ribosylation Factors / Q000096:biosynthesis*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D004041:Dietary Fats / Q000008:administration & dosage*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D020439:Growth Cones / Q000502:physiology; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
C511801:ARL8B protein, human; D004041:Dietary Fats; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D020727:ADP-Ribosylation Factors