Effective method for extraction of larvicidal component from leaves of Azadirachta indica and Artemisia annua Linn.
Leaves of Artemisia annua linn. and Azadirachta indica were extracted in petroleum ether and hexane respectively by different methods of extraction i.e. cold extraction, reflux extraction and soxhlet extraction. The crude extract obtained was tested against third instar larvae of Anopheles stephensi. On comparison of larval mortality of crude extract obtained by these three methods, both soxhlet and reflux extraction method showed 100% mortality at 200 ppm after 48 hr in case of A. annua. However LC50 (20 ppm) value of crude extract obtained by soxhlet extraction showed better results than reflux extraction (35 ppm) method after 72 hr. In case of A. indica, crude obtained by soxhlet showed 100% mortality (after 48 hr) at 250 ppm and LC50 of 69 ppm at 72 hr. Reflux extraction does not show any appreciable mortality even at 250 ppm concentration.
Tonk
Shobhita
S
Department of Chemistry, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra-282 005, India.
Bartarya
Roll
R
Maharaj Kumari
K
K
Bhatnagar
V P
VP
Srivastava
S S
SS
D000818:Animals; D000852:Anopheles / Q000187:drug effects* / Q000254:growth & development; D031172:Artemisia annua / Q000737:chemistry*; D031601:Azadirachta / Q000737:chemistry*; D007306:Insecticides / Q000302:isolation & purification* / Q000494:pharmacology; D007814:Larva / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*
D007306:Insecticides; D010936:Plant Extracts