[Study on endophyte and the growth stages of Ophiopogon japonicus].
OBJECTIVE
To study on the relationship between the endophyte and the life cycle of Ophiopogon japonicus in Zhejiang.


METHODS
Sample roots of Ophiopogon japonicus at different growth stages were thoroughly washed and cut into small fragments, then cleared (removing cytoplasmic contents from cells) using hot 10% KOH and stained with acid fuchsin (alternative stain). The hyphae, the arbuscular and the vesicular of endophyte were examined.


RESULTS
The hyphae appeared and grew in the seedling stage, the hyphae grew into arbuscular in the root tuber generating stage and vesicular in the stage of root tuber expanding period.


CONCLUSIONS
The endophytes in Ophiopogon japonicus appeared in forms of hyphae, arbuscular and vesicular at different growth stages to meet the needs of Ophiopogon japonicus developing.
Cheng
Dong-Qing
DQ
Department of Life Science, Zhejiang College of TCM, Hangzhou 310053, China. cdq@hztele.com.cn
Yu
Zhi-Ren
ZR
Pan
Pei-Lei
PL
Lin
Mei-Ai
MA
Chen
Yi-Tao
YT
D005658:Fungi / Q000648:ultrastructure; D031441:Ophiopogon / Q000254:growth & development* / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D010946:Plants, Medicinal / Q000254:growth & development* / Q000382:microbiology*; D027343:Rhizome / Q000254:growth & development / Q000382:microbiology; D012621:Seasons