fester, A candidate allorecognition receptor from a primitive chordate.
Histocompatibility in the primitive chordate, Botryllus schlosseri, is controlled by a single, highly polymorphic locus, the FuHC. By taking a forward genetic approach, we have identified a locus encoded near the FuHC, called fester, which is polymorphic, polygenic, and inherited in distinct haplotypes. Somatic diversification occurs through extensive alternative splicing, with each individual expressing a unique repertoire of splice forms, both membrane bound and potentially secreted, all expressed in tissues intimately associated with histocompatibility. Functional studies, via both siRNA-mediated knockdown and direct blocking by monoclonal antibodies raised against fester, were able to disrupt predicted histocompatibility outcomes. The genetic and somatic diversity, coupled to the expression and functional data, suggests that fester is a receptor involved in histocompatibility.
Nyholm
Spencer V
SV
Department of Pathology, Stanford University School of Medicine, California 94305, USA.
Passegue
Emmanuelle
E
Ludington
William B
WB
Voskoboynik
Ayelet
A
Mitchel
Katrina
K
Weissman
Irving L
IL
De Tomaso
Anthony W
AW
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003001:Cloning, Molecular; D015870:Gene Expression; D016678:Genome / Q000235:genetics; D023281:Genomics; D006648:Histocompatibility; D006649:Histocompatibility Antigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D034622:RNA Interference; D016415:Sequence Alignment; D014561:Urochordata / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D011506:Proteins