title: Nearest neighbor parameters for Watson-Crick complementary heteroduplexes formed between 2'-O-methyl RNA and RNA oligonucleotides.
abstract: Results from optical melting studies of Watson-Crick complementary heteroduplexes formed between 2'-O-methyl RNA and RNA oligonucleotides are used to determine nearest neighbor thermodynamic parameters for predicting the stabilities of such duplexes. The results are consistent with the physical model assumed by the individual nearest neighbor-hydrogen bonding model, which contains terms for helix initiation, base pair stacking and base pair composition. The sequence dependence is similar to that for Watson-Crick complementary RNA/RNA duplexes, which suggests that the sequence dependence may also be similar to that for other backbones that favor A-form RNA conformations.
authors.affiliation: Department of Chemistry, University of Rochester, RC Box 270216, Rochester, NY 14627, USA.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Kierzek
authors.forename: David H
authors.initials: DH
authors.lastname: Mathews
authors.forename: Anna
authors.initials: A
authors.lastname: Ciesielska
authors.forename: Douglas H
authors.initials: DH
authors.lastname: Turner
authors.forename: Ryszard
authors.initials: R
authors.lastname: Kierzek
mesh_terms: D020029:Base Pairing; D008745:Methylation; D009843:Oligoribonucleotides / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D009843:Oligoribonucleotides; D012330:RNA, Double-Stranded