Interplay between exchange protein directly activated by cAMP (Epac) and microtubule cytoskeleton.
"Exchange protein directly activated by cAMP" (Epac) is a newly discovered cAMP receptor that mediates the intracellular cAMP actions in addition to the classic cAMP-dependent protein kinase system. In this study, we show that Epac interacts directly with tubulin, co-purifies with cellular microtubules, and co-localizes with the mitotic spindle assembly. Association with microtubules suppresses Epac-mediated Rap1 activation, while the binding of Epac promotes microtubule formation. These results demonstrate that Epac plays an important role in connecting the microtubule cytoskeleton network and intracellular cAMP-signalling.
Mei
Fang C
FC
Department of Pharmacology and Toxicology, The University of Texas Medical Branch, Galveston, TX 77555, USA.
Cheng
Xiaodong
X
D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D002522:Chlorocebus aethiops; D000242:Cyclic AMP / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007118:Immunoassay; D013058:Mass Spectrometry; D008868:Microtubule Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D011953:Receptors, Cyclic AMP; D015398:Signal Transduction; D008941:Spindle Apparatus / Q000378:metabolism*; D014162:Transfection
D002352:Carrier Proteins; D020662:Guanine Nucleotide Exchange Factors; D008868:Microtubule Proteins; D010750:Phosphoproteins; C116122:RAPGEF3 protein, human; C046282:RNA polymerase II-binding proteins; D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases