title: Structures and serology of the O-antigens of Proteus strains classified into serogroup O17 and former serogroup O35.
abstract: BACKGROUND
  Bacteria of the genus Proteus are facultative pathogens which commonly cause urinary tract infections. Based on the serological specificity of the O-chain polysaccharide of the lipopolysaccharide (O-polysaccharide, O-antigen), strains of P. mirabilis and P. vulgaris have been classified into 60 serogroups. Studies on the chemical structure and serological specificity of the O-antigens aim at the elucidation of the molecular basis and improvement of the serological classification of these bacteria.
  
  
  METHODS
  The O-polysaccharide was prepared by acetic acid degradation of the lipopolysaccharide isolated from dried bacterial mass of each strain by hot phenol/water extraction. (1)H- and (13)C-NMR spectroscopy was used for structural studies. Serological studies were performed with rabbit O-antisera using enzyme immunosorbent assay, passive hemolysis test, and the inhibition of reactions in these assays as well DOC-PAGE and Western blot.
  
  
  RESULTS
  Four Proteus strains belonging to serogroups O17 and O35 were found to possess similar O-polysaccharide structures, in particular having the same carbohydrate backbone built up of tetrasaccharide repeating units. However, they differ in the presence or absence of additional substituents, such as phosphoethanolamine in P. mirabilis O17 and glucose in P. penneri O17, as well as in the pattern and degree of O-acetylation of various monosaccharide residues. Serological studies also showed close relationships between the O-antigens studied.
  
  
  CONCLUSIONS
  Based on these data it is proposed to reclassify strain P. mirabilis PrK 61/57, formerly representing the O35 serogroup, into the serogroup O17 in the Kauffman-Perch classification system of Proteus.
authors.affiliation: Department of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Torzewska
authors.forename: Sebastian
authors.initials: S
authors.lastname: Grabowski
authors.forename: Anna N
authors.initials: AN
authors.lastname: Kondakova
authors.forename: Filip V
authors.initials: FV
authors.lastname: Toukach
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Nikolay P
authors.initials: NP
authors.lastname: Arbatsky
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Antoni
authors.initials: A
authors.lastname: Rózalski
authors.forename: Wiesław
authors.initials: W
authors.lastname: Kaca
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry / Q000145:classification*; D011514:Proteus vulgaris / Q000737:chemistry / Q000145:classification* / Q000276:immunology; D012703:Serotyping
chemical_list: D019081:O Antigens