title: Conserved balance of hepatocyte nuclear DNA content in mononuclear and binuclear hepatocyte populations during the course of chronic viral hepatitis.
abstract: OBJECTIVE
  To analyze the percentages of hepatocytes with increased nuclear DNA content, i.e., tetraploid (4n) and octoploid (8n) nuclei, and then compared mononuclear and binuclear hepatocyte populations.
  
  
  METHODS
  The percentages of mononuclear diploid (2n), 4n, and 8n hepatocytes and those of binuclear 2 x 2n, 2 x 4n, and 2 x 8n hepatocytes were determined with a method that can simultaneously measure hepatocyte nuclear DNA content and binuclearity in 62 patients with chronic hepatitis B or C. The percentage of 4n and 8n hepatocytes in the mononuclear hepatocyte population was compared with the percentage of 2 x 4n and 2 x 8n hepatocytes in the binuclear hepatocyte population.
  
  
  RESULTS
  The percentages of 4n and 8n hepatocytes in mononuclear hepatocytes and 2 x 4n and 2 x 8n hepatocytes in binuclear hepatocytes were similar, regardless of the activity or fibrosis grade of chronic hepatitis and regardless of the infecting virus.
  
  
  CONCLUSIONS
  The distribution of nuclear DNA content within mononuclear and binuclear hepatocyte populations was conserved during the course of chronic viral hepatitis.
authors.affiliation: Department of Gastroenterology, Ogaki Municipal Hospital, 4-86 Minaminokawa, Ogaki, Gifu 503-8502, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Toyoda
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumada
authors.forename: Olivier
authors.initials: O
authors.lastname: Bregerie
authors.forename: Christian
authors.initials: C
authors.lastname: Brechot
authors.forename: Chantal
authors.initials: C
authors.lastname: Desdouets
mesh_terms: D000328:Adult; D002452:Cell Count; D002467:Cell Nucleus / Q000737:chemistry* / Q000473:pathology; D004247:DNA / Q000032:analysis*; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D019694:Hepatitis B, Chronic / Q000235:genetics* / Q000473:pathology; D019698:Hepatitis C, Chronic / Q000235:genetics* / Q000473:pathology; D022781:Hepatocytes / Q000737:chemistry / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011123:Polyploidy
chemical_list: D004247:DNA