[Isolation and properties of malate dehydrogenase from meso- and thermophilic bacteria].
A scheme of purification of malate dehydrogenase from Macromonas bipunctata strain D-405 and Vulcanithermus medioatlanticus DSM 14978T was developed. This scheme was used to obtain electrophoretically homogeneous enzyme preparations of the mesophilic bacterium M. bipunctata (specific activity, 26.9 +/- 0.8 U/mg protein; yield, 10.9%) and the thermophilic bacterium V. medioatlanticus (specific activity, 5.0 +/- 0.2 U/mg protein; yield, 19.2%). Using these high-purity enzymatic preparations, the physicochemical and regulatory properties of malate dehydrogenase were studied and the differences in kinetic characteristics and thermal stability of the preparations were determined.
Eprintsev
a T
aT
Falaleeva
M I
MI
Klimova
M A
MA
Parfenova
N V
NV
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D042521:Burkholderiaceae / Q000201:enzymology*; D006358:Hot Temperature; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000737:chemistry / Q000302:isolation & purification*; D013045:Species Specificity; D013824:Thermus / Q000201:enzymology*
D001426:Bacterial Proteins; D008291:Malate Dehydrogenase