title: Dormant cancer cells retrieved from metastasis-free organs regain tumorigenic and metastatic potency.
abstract: This study shows that solitary, dormant human cancer cells, retrieved from metastasis-free organs of animals carrying spontaneously metastatic primary tumors, can reactivate their tumorigenic and metastatic potency. The tumors were produced by MDA-MB-435 CL16 breast cancer cells permanently labeled with green fluorescent protein and the neomycin resistance gene. This enabled unequivocal identification of tumor cells emerging from organ explants cultured in neomycin to eliminate nonneoplastic host cells. Rescued cells resumed proliferation and generated lines that were tumorigenic and metastatic in fresh animals. All resulting primary and secondary tumors were uniformly labeled. Cells recovered from bone marrows and spleens, where there were no metastases, were as tumorigenic and metastatic as cells recovered from lungs and lymph nodes, which are the preferred sites of colonization for this tumor line. This evidence that malignant growth of disseminated cancer cells is suspended indefinitely by microenvironmental conditions in metastasis-free organs, although it is still active in others of the same host, shows that neoplastic progression can be arrested and has far-reaching biological and clinical implications. Specifically, it predicts the existence of natural, nonimmune host mechanisms that stimulate or inactivate tumor growth in different anatomical sites, which may be exploitable for therapeutic benefit.
authors.affiliation: Rebecca and John Moores Comprehensive Cancer Center and Department of Pathology, University of California, San Diego, 3855 Health Sciences Dr. MC0803, La Jolla, CA 92093-0803, USA.
authors.forename: Mika
authors.initials: M
authors.lastname: Suzuki
authors.forename: Evangeline Sari
authors.initials: ES
authors.lastname: Mose
authors.forename: Valerie
authors.initials: V
authors.lastname: Montel
authors.forename: David
authors.initials: D
authors.lastname: Tarin
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned; D006801:Humans; D051379:Mice; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D017077:Culture Media, Conditioned