Taura syndrome virus from Penaeus vannamei shrimp cultured in Korea.
Mass mortality occurred among Penaeus vannamei shrimp cultured in Korea in 2004. In an earlier study, we reported white spot syndrome virus (WSSV) as a causative agent of mass mortality of P. monodon shrimp in Korea (Moon et al. 2003; Dis Aquat Org 53:11-13). However, in the present study, we detected Taura syndrome virus (TSV) from the moribund 2004 P. vannamei shrimp by reverse transcription polymerase chain reaction (RT-PCR). In addition, during our regular screening for the TSV in stocks of P. vannamei imported from Hawaii, USA, we also detected TSV by RT-PCR. The nucleotide sequences of the partial capsid protein VP1 of 2 Korean isolates were 99% identical to each other and 96 to 99% identical to those of TSVs isolated from the Americas, Taiwan, and Thailand. Phylogenetic analysis revealed that the 2 Korean isolates were closely related to TSV types from Thailand. This is the first report on the detection of TSV during an epizootic among cultured P. vannamei in Korea, and our results suggests the possibility that TSV has been introduced via the imported stock of P. vannamei.
Do
Jeong Wan
JW
Pathology Division, National Fisheries Research & Development Institute, Kijang, Busan 619-902, ROK.
Cha
Seung Ju
SJ
Lee
Nam Sil
NS
Kim
Yi Cheong
YC
Kim
Jin Woo
JW
Kim
Jae Dong
JD
Park
Jeong Woo
JW
D000818:Animals; D017756:Aquaculture; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D007723:Korea; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000821:virology*; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004279:DNA, Viral