hSef co-localizes and interacts with Ras in the inhibition of Ras/MAPK signaling pathway.
To study the mechanism of the inhibitory effects of Sef (similar expression to fgf genes) on Ras/mitogen-activated protein kinase (MAPK) signaling pathway, we observed cellular localization of this protein. Immunofluorescent staining results show that Sef locates in the vesicles of the cytoplasm without bFGF treatment but co-localizes with Ras on the plasma membrane (PM) in response to bFGF stimulation. The coimmunoprecipitation assay demonstrates that Sef interacts with Ras or RasG12V, respectively. We observed that Sef inhibited FGF induced, but not RasG12V mediated, signal transduction. We propose that Sef interacted with Ras in the inhibition of Ras/MAPK signaling pathway.
Ren
Yongming
Y
Tsinghua Institute of Genome Research, Department of Biological Sciences and Biotechnology, and School of Medicine, Tsinghua University, Beijing 100084, China.
Cheng
Long
L
Rong
Zhili
Z
Li
Zhiyong
Z
Li
Yinghua
Y
Li
Hongge
H
Wang
Zhen
Z
Chang
Zhijie
Z
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D047468:Immunoprecipitation; D020935:MAP Kinase Signaling System / Q000502:physiology*; D018123:Receptors, Interleukin / Q000502:physiology*; D018631:ras Proteins / Q000502:physiology*
C477526:IL17RD protein, human; D018123:Receptors, Interleukin; D016222:Fibroblast Growth Factor 2; D018631:ras Proteins