title: [Sorcin overexpression and multidrug resistance in human gastric cancer cells].
abstract: OBJECTIVE
  To screen multidrug resistance (MDR) related proteins in human gastric cancer using proteomics technique.
  
  
  METHODS
  Two-dimensional electrophoresis (2-DE) was used to separate the total proteins of vincristine-resistant human gastric cancer cell line SGC7901/VCR and its counterpart SGC7901. PDQuest software was used to analyze 2-DE images, and the differential expression proteins between the 2 cell lines were identified by both MALDI-TOF-MS and ESI-Q-TOF. The differential expression level of sorcin, one of the identified proteins, was confirmed by western blot analysis. The effect of sorcin on the development of MDR of SGC7901/VCR was determined by antisense oligonucleotides (ASO) technique.
  
  
  RESULTS
  Sorcin as a high expression protein in SGC7901/VCR was identified and the suppression of sorcin expression by sorcin ASO could enhance the vincristine chemosensitivity in SGC7901/VCR.
  
  
  CONCLUSIONS
  Sorcin overexpression is related to MDR in human gastric cancer cell line SGC7901/VCR.
authors.affiliation: Key Laboratory of Cancer Proteomics of Ministry of Health of China, Xiangya Hospital, Central South University, Changsha.
authors.forename: Hong
authors.initials: H
authors.lastname: Yi
authors.forename: Yi-xuan
authors.initials: YX
authors.lastname: Yang
authors.forename: Can-e
authors.initials: CE
authors.lastname: Tang
authors.forename: Zhu-chu
authors.initials: ZC
authors.lastname: Chen
authors.forename: Gui-ying
authors.initials: GY
authors.lastname: Zhang
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Xiao
mesh_terms: D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008969:Molecular Sequence Data; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D014750:Vincristine / Q000494:pharmacology
chemical_list: D002135:Calcium-Binding Proteins; C503113:SRI protein, human; D014750:Vincristine