A simple and rapid determination of biapenem in plasma by high-performance liquid chromatography.
A simple, rapid and precise HPLC method using ultrafiltration to remove plasma protein was developed to determine biapenem concentrations in human plasma. Plasma was separated by centrifugation at 4 degrees C from blood collected in heparinized vacuum tubes, and biapenem was stabilized by immediate mixing the plasma with 1M 3-morpholinopropanesulfonic acid (MOPS) buffer (pH 7.0) (1:1). Biapenem was detected by ultraviolet absorbance at 300nm with no interfering plasma peak. The calibration curve of biapenem in human plasma was linear from 0.04 to 50microg/mL. The limit of detection was 0.01microg/mL, which was more than 40-fold lower than that of conventional plasma protein precipitation using ammonium sulfate. The assay has been clinically applied to pharmacokinetic studies in patients.
Ikeda
Kayo
K
Department of Clinical Pharmacotherapy, Division of Clinical Pharmacotherapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan. ikeda@hiroshima-u.ac.jp
Ikawa
Kazuro
K
Ikeda
Aki
A
Nishikawa
Yoshimi
Y
Morikawa
Norifumi
N
D000900:Anti-Bacterial Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013845:Thienamycins / Q000097:blood* / Q000493:pharmacokinetics
D000900:Anti-Bacterial Agents; D013845:Thienamycins; C065257:biapenem