Glucose biosensors as models for the development of advanced protein-based biosensors.
Glucose sensing is used as a model to explore the advantages and problems deriving from the use of either enzymes or sugar binding proteins to develop stable fluorescence biosensors. We report on a novel approach to address the problem of substrate consumption by sensors based on enzymes, namely the utilization of apo-enzymes as non-active forms of the protein which are still able to bind the substrate/ligand. We also review studies in which derivatization of a naturally thermostable sugar-binding protein with a fluorescent probe allows quantitative monitoring of glucose binding even after immobilization on a solid support.
Staiano
Maria
M
Institute of Protein Biochemistry, CNR, Italian National Research Council, Via Pietro Castellino, Naples, Italy.
Bazzicalupo
Paolo
P
Rossi
Mose'
M
D'Auria
Sabato
S
D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D003067:Coenzymes / Q000737:chemistry; D005453:Fluorescence; D005454:Fluorescence Polarization; D005941:Glucokinase / Q000737:chemistry; D007202:Indicators and Reagents; D008320:Maltose / Q000737:chemistry; D008958:Models, Molecular; D008991:Monitoring, Physiologic / Q000379:methods; D011506:Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D001786:Blood Glucose; D003067:Coenzymes; D007202:Indicators and Reagents; D011506:Proteins; D008320:Maltose; D005941:Glucokinase