Electrochemical detection and control of interactions between DNA and electroactive intercalator using a DNA-alginate complex film modified electrode.
The surface of a glassy carbon electrode was modified with a DNA-alginate complex film in which water-soluble DNA was encapsulated into a calcium-alginate gel. The resulting modified electrode (DAFE) was used to detect ethidium bromide (EtBr), after its accumulation on the electrode. The intercalative behavior of EtBr for dsDNA in the film was investigated by measuring the electrode response based on the intercalation of EtBr separated from nonspecific interactions (electrostatic interaction). The accumulation of EtBr in the dsDAF was enhanced by applying a negative potential below -200 mV at the dsDAFE. When a positive potential above +700 mV was applied to the dsDAFE for a constant time with stirring in a Tris buffer solution, the amount accumulated decreased. These results indicate that it is possible to electrochemically control the accumulation and release of EtBr when a dsDAFE is used. In addition, the accumulation and detection of EtBr in spiked river water samples and daunomycin, an antitumor agent, is described.
Matsumoto
Yuko
Y
Division of Environmental Material Science, Graduate School of Environmental Science, Hokkaido University, Sapporo, Hokkaido, 060-0810, Japan.
Terui
Norifumi
N
Tanaka
Shunitz
S
D000464:Alginates / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D003630:Daunorubicin / Q000032:analysis / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004996:Ethidium / Q000032:analysis* / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007364:Intercalating Agents / Q000032:analysis* / Q000737:chemistry; D045483:Rivers / Q000737:chemistry
D000464:Alginates; D006603:Hexuronic Acids; D007364:Intercalating Agents; D002244:Carbon; D020723:Glucuronic Acid; D004247:DNA; D004996:Ethidium; D003630:Daunorubicin