[Allelic loss of 6q16.3 microsatellite DNA in childhood acute lymphoblastic leukemia and bioinformatics analysis].
OBJECTIVE
To locate the cluster region of loss of heterozygosity (LOH) in children with acute lymphoblastic leukemia (ALL), and explore the new tumor suppressor gene.


METHODS
Allelic loss was analyzed by PCR with 15 microsatellite markers mapping on 6q16.3. The LOH was analyzed by bioinformatics. The relationship between LOH and clinical factors was further analyzed.


RESULTS
The frequency of LOH at least at one loci on 6q16.3 was 32.7%. The LOH in relapsed patients was higher than those in not relapsed. The higher frequency of LOH was observed in two regions of D6S1709-D6S1028 and D6S2160-D6S1580 at 6q16.3. GRIK2 may be a candidate of tumor suppressor gene. There are 12 ESTs may carry out new anti-oncogene. Patients with 6q LOH had higher WBC counts (P < 0.01), blast cells percentage (P < 0.01), relapse rate (P < 0.05) and chromosomal aberration (P < 0.05).


CONCLUSIONS
D6S1709-D6S1028 and D6S2160-D6S1580 are two regions of minimus deletion on 6q16.3 in which tumor suppressor gene may exist. The LOH on 6q16.3 may be a prognostic index of children with ALL.
Yang
Ming-hua
MH
Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China.
Cao
Li-zhi
LZ
Yu
Yan
Y
Zhang
Zhao-xia
ZX
Wang
Ying
Y
Kang
Rui
R
Chen
Ying
Y
Tan
Zhi-hong
ZH
Wu
Xiu-shan
XS
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D019295:Computational Biology; D006801:Humans; D007223:Infant; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*