The effect of the ionophore valinomycin on biomimetic solid supported lipid DPPTE/EPC membranes.
Self assembled monolayers and bilayers are produced on a flat glass surface, bound by a thiolipid onto bare gold. 1,2-Dipalmitoyl-sn-Glycero-3-Phosphothioethanol (DPPTE) is used as the molecule binding to the electrode surface. The lipid lambda-alpha-Phosphatidyl-Choline-beta-Oleoyl-g-Palmitoyl (POPC) and the lipid mixture eggphosphatadiylcholine (EPC) are used as spacer lipids with the aim of achieving solid-supported artificial lipid membranes. With the aim of creating and investigating more natural systems, ion carrier proteins such as valinomycin are introduced into the DPPTE/EPC system. The direct influence on the membranes as well as the effects of different ionic solutions on the proteins is shown.
Rose
Lars
L
Department of Chemistry, University of Bath, BA2 7AY, Bath, United Kingdom. lars.rose@nrc-cnrc.gc.ca <lars.rose@nrc-cnrc.gc.ca>
Jenkins
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AT
D040761:Biomimetic Materials / Q000737:chemistry*; D017097:Electric Impedance; D007476:Ionophores / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D010713:Phosphatidylcholines / Q000737:chemistry*; D014280:Triglycerides / Q000737:chemistry*; D014634:Valinomycin / Q000737:chemistry*
C520656:1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol; D007476:Ionophores; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D014280:Triglycerides; D014634:Valinomycin; C028694:1-palmitoyl-2-oleoylphosphatidylcholine