title: Over-expression of c-Myb increases the frequency of hemogenic precursors in the endothelial cell population.
abstract: Definitive hematopoiesis has been proposed to arise from hemogenic endothelial cells during mouse embryogenesis. The c-myb proto-oncogene is essential for the development of definitive hematopoiesis and was reported to be activated in hemogenic endothelial cells. To investigate whether c-Myb is involved in regulating the development of hemogenic endothelial cells, we conditionally induced c-myb over-expression during the in vitro differentiation of embryonic stem cells. VE-cadherin+ CD45- cells inducibly expressing c-Myb showed an increase in multilineage colony formation as well as an augmented capacity of the colony forming cells to self-renew in vitro under the condition that only the endogenous c-myb gene was expressed during differentiation of hematopoietic cells. Over-expression of c-Myb in the endothelial population led to activation of genes associated with definitive hematopoiesis such as Runx1, Hoxb4, Mll and Etv6. Our data provide evidence that c-Myb is able to exert an effect in endothelial cells which fosters the establishment of their hemogenic potential.
authors.affiliation: Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Guoyou
authors.initials: G
authors.lastname: Dai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Yuri
authors.initials: Y
authors.lastname: Shimoda
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Fujimoto
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Nishikawa
authors.forename: Minetaro
authors.initials: M
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D016923:Cell Death; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004627:Embryonic Induction / Q000235:genetics; D042783:Endothelial Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D020570:Genes, myb / Q000502:physiology*; D006410:Hematopoiesis / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D014162:Transfection