A validated liquid chromatographic method for the antiallergy agent CI-922 in dog and rat plasma.
A sensitive, specific and rapid liquid chromatographic procedure to selectively monitor CI-922 in dog and rat plasma was developed and validated. Plasma samples were acidified and extracted with ethyl ether. The organic layer was evaporated to dryness, reconstituted with mobile phase, and the analytes were separated and quantified on an octadecylsilane stationary phase (lambda = 340 nm). The procedure was linear from 0.05 to 3.0 mug ml(-1) with a detection limit of 0.02 mug ml(-1). The accuracy of the method had relative errors of 7.6, 4.2 and 5.4% for dog plasma controls containing 0.5, 1.50 and 2.5 mug CI-922 ml(-1), respectively. The corresponding precision was 5.0, 4.0 and 4.8% (RSD). Similarly, relative standard deviations less than 4.6% and relative errors of less than 1.4% were obtained for rat plasma controls. The method is suitable for pharmacology, toxicology and pharmacokinetic studies of CI-922.
Eiseman
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Warner-Lambert/Parke-Davis Pharmaceutical Research, Pharmacokinetics/Drug Metabolism Department, 2800 Plymouth Road, Ann Arbor, MI 48105, USA.
Reynolds
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Johnson
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Daftsios
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Pachla
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LA