Arabidopsis thaliana is an asymptomatic host of Alfalfa mosaic virus.
The susceptibility of Arabidopsis thaliana ecotypes to infection by Alfalfa mosaic virus (AMV) was evaluated. Thirty-nine ecotypes supported both local and systemic infection, 26 ecotypes supported only local infection, and three ecotypes could not be infected. No obvious symptoms characteristic of virus infection developed on the susceptible ecotypes under standard conditions of culture. Parameters of AMV infection were characterized in ecotype Col-0, which supported systemic infection and accumulated higher levels of AMV than the symptomatic host Nicotiana tabacum. The formation of infectious AMV particles in infected Col-0 was confirmed by infectivity assays on a hypersensitive host and by electron microscopy of purified virions. Replication and transcription of AMV was confirmed by de novo synthesis of AMV subgenomic RNA in Col-0 protoplasts transfected with AMV RNA or plasmids harboring AMV cDNAs.
Balasubramaniam
Muthukumar
M
Department of Botany and Plant Pathology, Lilly Hall of Life Sciences, Purdue University, West Lafayette, IN 47907, USA.
Ibrahim
Amr
A
Kim
Bong-Suk
BS
Loesch-Fries
L Sue
LS
D017794:Alfalfa mosaic virus / Q000472:pathogenicity / Q000502:physiology*; D017360:Arabidopsis / Q000145:classification / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D013045:Species Specificity; D014774:Virulence; D014779:Virus Replication