title: Conventional and laser induced fluorescence detection of glucuronic acid conjugates after derivatization and liquid chromatographic separation.
abstract: Pre-chromatographic derivatisation of the carboxylic acid function of glucuronic acid conjugates is a suitable method for the selective and ultra-sensitive analysis of these compounds in urine and plasma samples. This goal is achieved by applying an indirect derivatisation procedure and laser induced fluorescence detection with a homemade detection system equipped with a continuous-wave argon-ion laser. The minimum detectable amounts for the analytes, after derivatisation, are about 3 amol using the fluorescein fluorophore. In comparison with conventional induced fluorescence detection a gain in sensitivity of over four orders of magnitude is obtained.
authors.affiliation: Division of Analytical Chemistry, Center for Bio-Pharmaceutical Sciences, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Lingeman
authors.forename: U R
authors.initials: UR
authors.lastname: Tjaden
authors.forename: C M
authors.initials: CM
authors.lastname: van den Beld
authors.forename: J
authors.initials: J
authors.lastname: van der Greef