A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1.
A phosphopantetheinyl transferase (PPTase) gene (pfaE), cloned from the docosahexaenoic acid (DHA)-producing bacterium Moritella marina strain MP-1, has an open reading frame of 861 bp encoding a 287-amino acid protein. When the pfaE gene was expressed with pfaA-D, which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (EPA) derived from Shewanella pneumatophori SCRC-2738 in Escherichia coli, the recombinant produced 12% EPA of total fatty acids. This suggests that pfaE encodes a PPTase required for producing n-3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP-1.
Orikasa
Yoshitake
Y
Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan.
Nishida
Takanori
T
Hase
Akira
A
Watanabe
Kazuo
K
Morita
Naoki
N
Okuyama
Hidetoshi
H
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015525:Fatty Acids, Omega-3 / Q000096:biosynthesis*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D034221:Moritella / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017855:Transferases (Other Substituted Phosphate Groups) / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D015525:Fatty Acids, Omega-3; C082662:phosphopantetheinyl transferase; D017855:Transferases (Other Substituted Phosphate Groups)