A new double-stranded RNA binding protein (DRBP-120) is associated with double-stranded RNA-activated protein kinase (PKR).
Double-stranded RNA-activated protein kinase (PKR) plays an important role in interferon-induced antiviral responses, and is also involved in intracellular signaling pathways, including the apoptosis, proliferation, and transcription pathways. In this study, a new 120-kDa PKR-associated protein designated double-stranded RNA binding protein (DRBP)-120 was identified using co-immunoprecipitation with anti-PKR antiserum and two-dimensional electrophoresis. Furthermore, DRBP-120 is a double-stranded RNA (dsRNA)-binding protein, and it was detected in both the cytoplasm and the nucleus of HeLa cells associated with PKR.
Watanabe
Shuji
S
Department of Physiology, School of Medicine, Iwate Medical University, Morioka 020-8505, Japan.
Yamashita
Tetsuro
T
Taira
Hideharu
H
D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D050779:Nuclear Factor 90 Proteins / Q000378:metabolism; D011485:Protein Binding; D012330:RNA, Double-Stranded / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D019892:eIF-2 Kinase / Q000378:metabolism*
C429643:ILF3 protein, human; D050779:Nuclear Factor 90 Proteins; D012330:RNA, Double-Stranded; D016601:RNA-Binding Proteins; D019892:eIF-2 Kinase