title: [Developing of a new multicolor-fluorescent labeled STR amplification kit].
abstract: OBJECTIVE
  To develop a PCR-based STR system for genotyping of 18 loci (Amelogenin, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, D7S820, D2S1338, D19S433, D12S391 and D19S253).
  
  
  METHODS
  By using primers labeled with four color fluorescent (FAM, HEX, TAMRA and ROX), two multiplex amplification reaction systems were developed to genotype Amelogenin and 17 STR loci.
  
  
  RESULTS
  Amelogenin and these 17 STR loci were genotyped successfully in different kinds of biological samples by the kit.
  
  
  CONCLUSIONS
  The STR amplification kit developed in our study gives a new approach to genotype these 18 loci in a efficient, steady and reliable way.
authors.affiliation: Institute of Forensic Sciences, Ministry of Justice, P.R. China, Shanghai 200063, China. anneinchina@163.com
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Chen
authors.forename: Cheng-Tao
authors.initials: CT
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Ting-Zhi
authors.initials: TZ
authors.lastname: Que
authors.forename: Zhen-Min
authors.initials: ZM
authors.lastname: Zhao
authors.forename: Ya-Xin
authors.initials: YX
authors.lastname: Wang
mesh_terms: D000483:Alleles; D000818:Animals; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D053803:Forensic Genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
chemical_list: D017931:DNA Primers; D007202:Indicators and Reagents