Laminin peptide-conjugated chitosan membrane: Application for keratinocyte delivery in wounded skin.
Tissue engineering requires the delivery and survival of cells to organ sites needing repair. Previously, we showed that an active laminin peptide (AG73: RKR-LQVQLSIRT)-conjugated chitosan membrane promoted cell adhesion and spreading in vitro. Here, we seeded human keratinocytes onto AG73-chitosan membranes and found that nearly 80% of the cells were attached to the membranes within 2 h. The membranes carrying the keratinocytes were inverted and placed onto exposed muscle fascia on the backs of nude mice. After 3 days, the keratinocytes had migrated from the membrane and established a stratified epidermis-like structure on the fascia. Cells recognize the AG73 through transmembrane proteoglycan syndecans, which recognition system has not previously been tested in tissue engineering applications. We suggest that the AG73-chitosan membrane is useful as a therapeutic formulation and is applicable as a cell delivery system such as delivering keratinocytes to a wound bed.
Ikemoto
Shigehiro
S
Department of Plastic and Aesthetic Surgery, Kitasato University School of Medicine, Sagamihara, Japan.
Mochizuki
Mayumi
M
Yamada
Masanori
M
Takeda
Akira
A
Uchinuma
Eiju
E
Yamashina
Shohei
S
Nomizu
Motoyoshi
M
Kadoya
Yuichi
Y
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D017690:Cell Transplantation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D007797:Laminin / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D004337:Drug Carriers; D007797:Laminin; D010446:Peptide Fragments; D048271:Chitosan