The determination of torasemide and metabolites in plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic method for the determination of torasemide and two active metabolites is described. The assay uses a reversed-phase gradient system and UV-detection. Sample preparation includes deproteinisation and liquid-solid extraction incorporating an internal standard. Data on recovery, detection limits, precision and accuracy are presented. The presence of an unidentified metabolite also is reported.
Besenfelder
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Boehringer Mannheim GmbH, Chemical Research Division, Bioanalytical Department, D-6800 Mannheim 31, Sandhofer Str. 116, West Germany.