The ADP/ATP ratio: A novel predictive assay for quality assessment of isolated pancreatic islets.
The current standard assays for islet product release criteria are unable to predict the outcome after clinical islet transplantation. Therefore, establishment of reliable and rapid assays enabling pre-transplantation prediction of islet potency is warranted. In the present study, we have evaluated the adenosine diphosphate (ADP)/adenosine triphosphate (ATP) test, the glucose-stimulated insulin release, the loss of islets during the first 24 h in culture, and the insulin/deoxyribonucleic acid as predictive assays for the ability of isolated porcine islets to cure athymic mice with streptozotocin-induced diabetes. From the results presented, it is concluded that the measurement of the ADP/ATP ratio was the only test that correlated with transplantation outcome. In summary, we propose that the ADP/ATP assay is worthwhile as applied to human islet transplantation and seek to validate it as a rapid and potent predictor of transplant outcome.
Goto
M
M
Tohoku University, Biomedical Engineering Research Organization, Sendai, Japan. gotpkichi@aol.com
Holgersson
J
J
Kumagai-Braesch
M
M
Korsgren
O
O
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D006801:Humans; D006943:Hyperglycemia / Q000175:diagnosis / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011183:Postoperative Complications; D011379:Prognosis; D013552:Swine
D015415:Biomarkers; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate