title: Effects of a natural extract from Mangifera indica L, and its active compound, mangiferin, on energy state and lipid peroxidation of red blood cells.
abstract: Following oxidative stress, modifications of several biologically important macromolecules have been demonstrated. In this study we investigated the effect of a natural extract from Mangifera indica L (Vimang), its main ingredient mangiferin and epigallocatechin gallate (EGCG) on energy metabolism, energy state and malondialdehyde (MDA) production in a red blood cell system. Analysis of MDA, high energy phosphates and ascorbate was carried out by high performance liquid chromatography (HPLC). Under the experimental conditions, concentrations of MDA and ATP catabolites were affected in a dose-dependent way by H2O2. Incubation with Vimang (0.1, 1, 10, 50 and 100 microg/mL), mangiferin (1, 10, 100 microg/mL) and EGCG (0.01, 0.1, 1, 10 microM) significantly enhances erythrocyte resistance to H2O2-induced reactive oxygen species production. In particular, we demonstrate the protective activity of these compounds on ATP, GTP and total nucleotides (NT) depletion after H2O2-induced damage and a reduction of NAD and ADP, which both increase because of the energy consumption following H2O2 addition. Energy charge potential, decreased in H2O2-treated erythrocytes, was also restored in a dose-dependent way by these substances. Their protective effects might be related to the strong free radical scavenging ability described for polyphenols.
authors.affiliation: Department of Biomedical Research, Center of Pharmaceutical Chemistry, 200 y 21 Atabey Playa, P.O. Box 16042, Havana, Cuba.
authors.forename: Janet
authors.initials: J
authors.lastname: Rodríguez
authors.forename: Donato
authors.initials: D
authors.lastname: Di Pierro
authors.forename: Magda
authors.initials: M
authors.lastname: Gioia
authors.forename: Susanna
authors.initials: S
authors.lastname: Monaco
authors.forename: René
authors.initials: R
authors.lastname: Delgado
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Coletta
authors.forename: Stefano
authors.initials: S
authors.lastname: Marini
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D031022:Mangifera / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D044004:Xanthones / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C501277:Mangifera indica extract; D010936:Plant Extracts; D044004:Xanthones; C013592:mangiferin; D008315:Malondialdehyde; D002392:Catechin; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate; D001205:Ascorbic Acid