Anticoagulant property of a semi-synthesized sodium beta-1,4-glucan sulfate.
OBJECTIVE
To investigate the anticoagulant efficacy and mechanism of a semi-synthesized sodium beta-1,4-glucan sulfate (Na-MCS).


METHODS
Anticoagulant activity was evaluated by means of coagulation assays in comparison with heparin. The anticoagulant mechanism of Na-MCS was disclosed by inhibitory analysis of the activities of coagulation factors using chromogenic substrates.


RESULTS
0.6 microg x mL(-1) Na-MCS could significantly prolong APTT and TT, but has less effect on PT at an even higher concentration. The dosage of Na-MCS required to double APTT of normal human plasma was 0.7 microg x mL(-1), lower than that of heparin with the activity of 150 u x mg(-1).


CONCLUSIONS
Na-MCS represented a potent anticoagulation activity in vitro, which matched the efficacy of heparin in a certain range of concentrations. Na-MCS exhibited anticoagulant activity due to inhibition of the coagulation factors IIa and Xa by the mediation of anti-thrombin AT-III.
Wang
Zhao-mei
ZM
Institute of Light Industry and Chemical Engineering, South China University of Technology, Guangzhou. wangzm@scut.edu.cn
Li
Lin
L
Li
Bing
B
Guo
Si-yuan
SY
D000925:Anticoagulants / Q000008:administration & dosage / Q000494:pharmacology*; D000990:Antithrombin III / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015951:Factor Xa / Q000378:metabolism; D005936:Glucans / Q000008:administration & dosage / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D010314:Partial Thromboplastin Time; D011516:Prothrombin / Q000378:metabolism; D011517:Prothrombin Time; D013918:Thrombin Time
D000925:Anticoagulants; D005936:Glucans; D000990:Antithrombin III; D011516:Prothrombin; C025337:Factor IIa; D006493:Heparin; C040088:1,4-glucan; D015951:Factor Xa