N-hexacosanol reverses diabetic induced muscarinic hypercontractility of ileum in the rat.
Diabetic neuropathy, a major complication of diabetes mellitus, is associated with development of gastrointestinal motility dysfunction and autonomic neuropathy. N-hexacosanol has neurotrophic effects and exhibits a wide variety of biological actions. In this study, we investigated the effects of cyclohexenonic long-chain fatty alcohol (N-hexacosanol) on streptozotocin-diabetic hypercontractility in the rat ileum longitudinal muscles. Treatment with N-hexacosanol did not alter the diabetic status of the animals, i.e., body weight, serum glucose, and serum insulin levels, but significantly restored the thickness of intestine wall and ameliorated diabetes-induced hypercontractility of the rat ileum in a dose-dependent manner. Furthermore, N-hexacosanol reversed the diabetes-induced upregulation of intestinal muscarinic M(2) and M(3) receptors mRNAs in the streptozotocin-diabetic rats. These results indicate that N-hexacosanol has therapeutic effects on hypercontractility in the diabetic ileum by ameliorating overexpression of muscarinic M(2) and M(3) receptors mRNAs.
Shinbori
Chiko
C
Department of Pathophysiological and Therapeutic Science, Division of Molecular Pharmacology, Tottori University Faculty of Medicine, 86 Nishimachi, Yonago, 683-8503 Japan.
Saito
Motoaki
M
Kinoshita
Yukako
Y
Satoh
Itaru
I
Kono
Tomoharu
T
Hanada
Takuya
T
Nanba
Eiji
E
Adachi
Kaori
K
Suzuki
Hiroto
H
Yamada
Masashi
M
Satoh
Keisuke
K
D000818:Animals; D002118:Calcium / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003929:Diabetic Neuropathies / Q000188:drug therapy*; D005233:Fatty Alcohols / Q000494:pharmacology* / Q000627:therapeutic use; D007082:Ileum / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043585:Receptor, Muscarinic M2 / Q000187:drug effects* / Q000235:genetics; D043587:Receptor, Muscarinic M3 / Q000187:drug effects* / Q000235:genetics; D013311:Streptozocin
D005233:Fatty Alcohols; D043585:Receptor, Muscarinic M2; D043587:Receptor, Muscarinic M3; D013311:Streptozocin; C051942:1-hexacosanol; D002118:Calcium