An efficient genetic screen in Drosophila to identify nuclear-encoded genes with mitochondrial function.
We conducted a screen for glossy-eye flies that fail to incorporate BrdU in the third larval instar eye disc but exhibit normal neuronal differentiation and isolated 23 complementation groups of mutants. These same phenotypes were previously seen in mutants for cytochrome c oxidase subunit Va. We have molecularly characterized six complementation groups and, surprisingly, each encodes a mitochondrial protein. Therefore, we believe our screen to be an efficient method for identifying genes with mitochondrial function.
Liao
T S Vivian
TS
Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.
Call
Gerald B
GB
Guptan
Preeta
P
Cespedes
Albert
A
Marshall
Jamie
J
Yackle
Kevin
K
Owusu-Ansah
Edward
E
Mandal
Sudip
S
Fang
Q Angela
QA
Goodstein
Gelsey L
GL
Kim
William
W
Banerjee
Utpal
U
D019883:Alkyl and Aryl Transferases / Q000235:genetics; D000818:Animals; D001126:Arginine-tRNA Ligase / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods; D003433:Crosses, Genetic; D004330:Drosophila / Q000235:genetics*; D004625:Embryo, Nonmammalian; D005123:Eye / Q000196:embryology / Q000254:growth & development; D005260:Female; D005820:Genetic Testing / Q000379:methods*; D019476:Insect Proteins / Q000235:genetics*; D008190:Lyases / Q000235:genetics; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000096:biosynthesis* / Q000235:genetics; D008954:Models, Biological; D009154:Mutation; D019884:Nitrogenous Group Transferases / Q000235:genetics
D019476:Insect Proteins; D024101:Mitochondrial Proteins; D019883:Alkyl and Aryl Transferases; C021386:4-hydroxybenzoate polyprenyltransferase; D019884:Nitrogenous Group Transferases; C047694:glutamyl-tRNA(Gln) amidotransferase; D008190:Lyases; C031581:cytochrome C synthetase; D001126:Arginine-tRNA Ligase