[High effectiveness of the method with cefpirome in detection of extended-spectrum beta-lactamases in different species of gram-negative bacilli].
Gram-negative bacilli were examined for ESBL production by using four methods: double-disc synergy diffusion test (DDST), and three tests of combined discs with cefpodoxime, ceftazidime and cefotaxime alone and the same cephalosporins with clavulanic acid. Strains determined as ESBL-negative with all these tests were examined by using fifth method with cefpirome. 47,5% from 178 negative in other methods strains, appeared ESBL-positive in this test. The examined strains belonged to 16 different species. Most of them were Enterobacter cloaceae, Serratia marcescens, Pseudomonas aeruginosa and Acinetobacter baumanii. It seems that the combined discs method with cefpirome may be usefull for phenotypic detection of ESBL producing bacteria also in the case of strains where ESBL production is camouflaged with derepressed chromosomal AmpC beta-lactamases.
Młynarczyk
Grazyna
G
Katedra i Zakład Mikrobiologii Lekarskiej AM w Warszawie.
Młynarczyk
Andrzej
A
Bilewska
Agata
A
Dukaczewska
Agata
A
Goławski
Cezary
C
Kicman
Agnieszka
A
Pupek
Julia
J
Serafin
Iwona
I
Luczak
Mirosław
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques / Q000379:methods; D001431:Bacteriological Techniques / Q000379:methods; D002439:Cefotaxime / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology*; D019818:Clavulanic Acid / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests / Q000379:methods; D013045:Species Specificity; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis*; D000097572:Cefpirome
D000900:Anti-Bacterial Agents; D002439:Cefotaxime; D002511:Cephalosporins; D019818:Clavulanic Acid; D001618:beta-Lactamases; D000097572:Cefpirome