Conventional and laser induced fluorescence detection of glucuronic acid conjugates after derivatization and liquid chromatographic separation.
Pre-chromatographic derivatisation of the carboxylic acid function of glucuronic acid conjugates is a suitable method for the selective and ultra-sensitive analysis of these compounds in urine and plasma samples. This goal is achieved by applying an indirect derivatisation procedure and laser induced fluorescence detection with a homemade detection system equipped with a continuous-wave argon-ion laser. The minimum detectable amounts for the analytes, after derivatisation, are about 3 amol using the fluorescein fluorophore. In comparison with conventional induced fluorescence detection a gain in sensitivity of over four orders of magnitude is obtained.
Lingeman
H
H
Division of Analytical Chemistry, Center for Bio-Pharmaceutical Sciences, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Tjaden
U R
UR
van den Beld
C M
CM
van der Greef
J
J