Coupling of ORL1 (NOP) receptor to G proteins is decreased in the nucleus accumbens of anxious relative to non-anxious mice.
We studied the involvement of endogenous ORL1 (NOP) receptors in the anxiety state. In mice selected as "anxious" and "non-anxious", ORL1 (NOP) receptor has been analysed by means of two autoradiographic approaches: [3H]nociceptin binding and nociceptin-stimulated [35S]GTPgammaS binding. We show that differences in anxiety state are associated with differences in G protein coupling efficiency of ORL1 (NOP) receptor in the nucleus accumbens, without any change in the density of the receptors.
Le Maître
Erwan
E
Unité de Neuropsychopharmacologie de la Dépression, Faculté de Médecine-Pharmacie, Rouen Cedex 1, France.
Daubeuf
François
F
Duterte-Boucher
Dominique
D
Costentin
Jean
J
Leroux-Nicollet
Isabelle
I
D000818:Animals; D001007:Anxiety; D001345:Autoradiography / Q000379:methods; D001522:Behavior, Animal; D004195:Disease Models, Animal; D019204:GTP-Binding Proteins / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000493:pharmacokinetics; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D051379:Mice; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism*; D018847:Opioid Peptides / Q000493:pharmacokinetics; D010759:Phosphorus Isotopes / Q000493:pharmacokinetics; D011957:Receptors, Opioid / Q000378:metabolism*; D014316:Tritium / Q000493:pharmacokinetics; D000094942:Nociceptin Receptor; D000097629:Nociceptin
D019204:GTP-Binding Proteins; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D018847:Opioid Peptides; D010759:Phosphorus Isotopes; D011957:Receptors, Opioid; D014316:Tritium; D000094942:Nociceptin Receptor; D000097629:Nociceptin; C000722287:Oprl1 protein, mouse