In vitro free radical scavenging activities and effect of synthetic oligosaccharides on antioxidant enzymes and lipid peroxidation in aged mice.
In this study we synthesized oligosaccharides using glucose as reactant via a route assisted by microwave irradiation and evaluated their antioxidant activity in vivo and in vitro. The results show that the oligosaccharides exhibited antioxidant activity in vitro as compared to standard antioxidants such as butylated hydroxytoluene (BHT), and alpha-tocopherol. This antioxidant activity depended on concentration and increased with increasing dose of sample. In addition, increased endogenous lipid peroxidation and decreased total antioxidant capacity (TAOC) were observed in aged mice. Thirty-day intraperitoneal administration of the oligosaccharides significantly decreased the lipid peroxidation in a dose-dependent manner. Oligosaccharides treatment increased TAOC and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in all organs tested in aged mice. The present study suggests that the synthetic oligosaccharides possess promising future for their strong free radical scavenging activity. Therefore they can be employed in compensating the decline in TAOC and the activities of antioxidant enzymes and in reducing the risks of lipid peroxidation.
Li
Xin-Ming
XM
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi, Jiangsu 214036, PR China.
Shi
Yong-Hui
YH
Wang
Fang
F
Wang
Hai-Song
HS
Le
Guo-Wei
GW
D000375:Aging / Q000378:metabolism*; D000465:Algorithms; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D051379:Mice; D008872:Microwaves; D009844:Oligosaccharides / Q000494:pharmacology*; D010851:Picrates / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009844:Oligosaccharides; D010851:Picrates; D013481:Superoxides; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase