Drosophila Ecp is a novel ribosome associated protein interacting with dRPL5.
Drosophila Ecp is a phylogenetically conserved protein with homologs in most eukaryotes. Studies on Ecp homologs in rat and human suggest those proteins might be involved in cell cycle control, cell proliferation, and learning and memory. However, the molecular function of Ecp itself remains unclear. We show that both the mRNA and protein of ecp are ubiquitously expressed during the entire fly embryogenesis and life cycle. Results of co-immunoprecipitation show that Ecp forms a stable complex with many ribosomal proteins, including dRPL5. The binding of Ecp to dRPL5 was confirmed by GST pulldown. Furthermore, Ecp was found to cosediment with ribosome subunits in a sucrose gradient. These results indicate that Ecp might be a novel ribosome associated protein interacting with dRPL5.
Wang
Daoyong
D
Department of Genetics and Developmental Biology, Genetics Research Center, Southeast University Medical School, Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Nanjing 210009, Jiangsu Province, China.
Xu
Min
M
Liu
Jiabin
J
Wan
Yongqi
Y
Deng
Haiteng
H
Dou
Fei
F
Xie
Wei
W
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D040061:Eukaryotic Initiation Factor-5; D018507:Gene Expression Regulation, Developmental; D010448:Peptide Initiation Factors / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D000906:Antibodies; D029721:Drosophila Proteins; D040061:Eukaryotic Initiation Factor-5; C520428:Krasavietz protein, Drosophila; D010448:Peptide Initiation Factors; D012269:Ribosomal Proteins; C029742:ribosomal protein L5