title: Purification of humanized monoclonal antibodies by membrane-based hybrid bioseparation technique.
abstract: We describe a rapid, inexpensive and scalable hybrid bioseparation technique for one-step purification of humanized monoclonal antibodies from mammalian cell culture supernatant. It involves the selective and reversible retention of the monoclonal antibody within a membrane module utilizing a combination of microfiltration of precipitated antibody and simultaneous hydrophobic interaction-based membrane chromatography of soluble antibody on the same microfiltration membrane. The retained monoclonal antibody is subsequently recovered from the membrane module in a highly pure form by changing the solution condition to that which favours simultaneous antibody dissolution and elution from the membrane. This combination of separation principles results in high-capacity, high-resolution separation. The monoclonal antibody purities and recoveries obtained in the experiments described in this paper were very high.
authors.affiliation: Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4L7.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Dharmesh M
authors.initials: DM
authors.lastname: Kanani
authors.forename: Raja
authors.initials: R
authors.lastname: Ghosh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification*; D016466:CHO Cells; D002678:Chimera; D002846:Chromatography, Affinity / Q000379:methods*; D006224:Cricetinae; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007158:Immunologic Techniques
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media