The effect of micropores in the surface of temperature-responsive culture inserts on the fabrication of transplantable canine oral mucosal epithelial cell sheets.
Primary canine oral mucosal epithelial cells were cultured on temperature-responsive dishes and cell culture inserts to fabricate transplantable epithelial cell sheets. When 3T3 feeder layers and fetal bovine serum were eliminated from dish culture, the harvested cell sheets became significantly more fragile. In contrast, when epithelial cells were cultured on inserts having submicron-scale pores, cell sheet fragility was eliminated. Keratin expression profiles showed no differences among the harvested cell sheets, but the expression of p63, a putative stem/progenitor marker, was strongly dependent on the presence of 3T3 feeder layers and serum. These results suggest that the maintenance of stem/progenitor cells is influenced by the apical/basal supply of nutrients as well as culture supplements.
Murakami
Daisuke
D
Graduate School of Science and Engineering, Waseda University, Tokyo 169 0051, Japan.
Yamato
Masayuki
M
Nishida
Kohji
K
Ohki
Takeshi
T
Takagi
Ryo
R
Yang
Joseph
J
Namiki
Hideo
H
Okano
Teruo
T
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology* / Q000637:transplantation*; D007150:Immunohistochemistry; D008297:Male; D009061:Mouth Mucosa / Q000166:cytology* / Q000378:metabolism; D016062:Porosity; D016038:Skin Transplantation; D013499:Surface Properties; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*