title: Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays.
abstract: The PDZ domain is a protein-protein interaction module that interacts with a C-terminal short peptide motif in its binding partners. A variety of methods have been used to study PDZ domain interactions. This chapter details the two methods most commonly used in the analysis of PDZ interactions: yeast two-hybrid and coimmunoprecipitation assays. In addition, we discuss the features that must be considered for an efficient analysis of PDZ interactions.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Lee
authors.forename: Jaewon
authors.initials: J
authors.lastname: Ko
authors.forename: Eunjoon
authors.initials: E
authors.lastname: Kim
mesh_terms: D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D011993:Recombinant Fusion Proteins