[Anti-schistosomiasis effect induced by full length DNA vaccine coding paramyosin of Schistosoma japonicum in C57BL/6 mice].
OBJECTIVE
To observe the anti-schistosomiasis effect in mice immunized with Sjc97 DNA vaccine.


METHODS
C57BL/6 mice were vaccinated intramuscularly with the Sjc97 DNA twice at an interval of 3 weeks, and challenged with 30 +/- 2 cercariae of Schistosoma japonicum three weeks after immunization. Mice in blank plasmid vector control and infection control groups were also infected with same number of cercariae. The mice were sacrificed 7 weeks after challenge infection. The size of single egg granulomas in livers was measured with micrometer. The level of hyaluronic acid (HA) and laminin (LN) in sera of the mice was determined by ELISA. PCR-ELISA was used to examine the expression of TGF-beta1 mRNA in liver.


RESULTS
The mean hepatic egg granuloma diameters of the three groups, the Sjc97 DNA, blank plasmid vector and infection control, were 183.75 +/- 42.36 microns, 303.12 +/- 37.36 microns and 304.38 +/- 53.23 microns, respectively. The hepatic granuloma was significantly smaller in the Sjc97 DNA group than that in control. The level of HA and LN in sera of Sjc97 DNA vaccinated mice was markedly lower than those in the two control groups (P < 0.01). The amount of TGF-beta1 mRNA isolated from the livers of mice in Sjc97 DNA group decreased significantly.


CONCLUSIONS
The results showed that Sjc97 DNA vaccine may act as an effective inhibitor against formation of egg granuloma and reduce immunopathological damage caused by Schistosoma japonicum in the host.
Chen
Jia-xu
JX
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
Liu
Shu-xian
SX
Cao
Jian-ping
JP
Song
Guang-cheng
GC
Xu
Yu-xin
YX
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006820:Hyaluronic Acid / Q000097:blood; D007114:Immunization; D007797:Laminin / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D014335:Tropomyosin / Q000235:genetics*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use
D007797:Laminin; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D014335:Tropomyosin; D019444:Vaccines, DNA; D006820:Hyaluronic Acid