title: Similar functional activity of dendritic cells recruited to the mesenteric lymph nodes of newborn and adult mice after the rectal delivery of Mycobacterium bovis BCG.
abstract: BCG rectal administration to newborn and adult mice induced protective immune responses against tuberculosis. BCG reaches the sub-epithelial site and the draining mesenteric lymph nodes (MLNs), and dendritic cells (DC) could be recruited to these sites. Using polarized Caco-2 epithelial cells, we showed that BCG translocates epithelial cells to basolateral compartment. Delayed in newborn BALB/c mice, an important recruitment of CD11c+ DCs, was documented in the rectal lamina propria and the MLNs during the first two weeks after rectal BCG delivery. In MLNs, two major DC subtypes were observed: conventional DCs (cDCs) (B220-) and plasmacytoid DCs (pDCs) (B220+). CIRE, mouse DC-specific intracellular adhesion molecule 3 grabbing non-integrin (DC-SIGN) is predominantly expressed on pDCs and at a higher level on pDCs from the adult compared to newborn MLNs. cDCs with a higher capacity to induce the proliferation of naïve CD4+ T cells than pDCs, triggered CD4+ T cells to produce interferon-gamma whereas pDCs triggered them to release interleukin-10. Both DC subtypes equilibrates T cells as a source of microbicidal/microbiostatic signals and those acting as source of counter-inflammatory signals, preventing tissue damage and/or accelerating tissue repair. Thus, rectal delivery of BCG could be a safe and efficient route of vaccination against tuberculosis.
authors.affiliation: Laboratoire de Référence des Mycobactéries, Institut Pasteur, 75015 Paris, France.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abolhassani
authors.forename: Micheline
authors.initials: M
authors.lastname: Lagranderie
authors.forename: Irina
authors.initials: I
authors.lastname: Caminshi
authors.forename: Felix
authors.initials: F
authors.lastname: Romain
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Balazuc
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Wagner
authors.forename: Myriam
authors.initials: M
authors.lastname: Tanguy
authors.forename: Laurence
authors.initials: L
authors.lastname: Fiette
authors.forename: Iradj
authors.initials: I
authors.lastname: Sobhani
authors.forename: Geneviève
authors.initials: G
authors.lastname: Milon
authors.forename: Gilles
authors.initials: G
authors.lastname: Marchal
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D000831:Animals, Newborn; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology* / Q000493:pharmacokinetics; D018845:CD11 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018938:Caco-2 Cells; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology*; D019402:Macrophage Inflammatory Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012007:Rectum / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control*
chemical_list: D001500:BCG Vaccine; C108355:CCL20 protein, human; D018845:CD11 Antigens; D054418:Chemokine CCL20; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D007371:Interferon-gamma