Blocking adherence of uropathogenic Escherichia coli isolate to HEP-2 cells and bladder of mice in the presence of antibody against p-fimbriae.
Presence of fimbriae on Escherichia coli isolated from the urine of patients with urinary tract infection was related to the ability of the bacteria to attach to human uroepithelial cells. One of the 50 isolates that expresses high MRHA p-fimbriae, selected and antibody against p-fimbriae from it, showed blocking of attachment of bacteria to HEP-2 cell in 1:1024 titer. Also, 1:512 titer of this antiserum to blocking of attachment in bladder tissue of mice is significant.
Bidhendi
S Moradi
SM
Bacteriology Department, Tarbiat Modarres University, Tehran, Iran.
Sattari
M
M
Pourbakhsh
S A
SA
Mobarez
A
A
Vandyousefi
J
J
Khaki
P
P
Heidari
M H
MH
Kazemnejad
A
A
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000276:immunology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000652:urine; D005260:Female; D036781:Fimbriae Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000652:urine; D010861:Fimbriae, Bacterial / Q000276:immunology; D006801:Humans; D007830:Larynx / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D008815:Mice, Inbred Strains; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D011817:Rabbits; D001743:Urinary Bladder / Q000276:immunology / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000382:microbiology / Q000652:urine
D000906:Antibodies; D036781:Fimbriae Proteins