[Developing of a new multicolor-fluorescent labeled STR amplification kit].
OBJECTIVE
To develop a PCR-based STR system for genotyping of 18 loci (Amelogenin, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, D7S820, D2S1338, D19S433, D12S391 and D19S253).


METHODS
By using primers labeled with four color fluorescent (FAM, HEX, TAMRA and ROX), two multiplex amplification reaction systems were developed to genotype Amelogenin and 17 STR loci.


RESULTS
Amelogenin and these 17 STR loci were genotyped successfully in different kinds of biological samples by the kit.


CONCLUSIONS
The STR amplification kit developed in our study gives a new approach to genotype these 18 loci in a efficient, steady and reliable way.
Li
Li
L
Institute of Forensic Sciences, Ministry of Justice, P.R. China, Shanghai 200063, China. anneinchina@163.com
Chen
Guang-Hui
GH
Li
Cheng-Tao
CT
Liu
Yan
Y
Lin
Yuan
Y
Que
Ting-Zhi
TZ
Zhao
Zhen-Min
ZM
Wang
Ya-Xin
YX
D000483:Alleles; D000818:Animals; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D053803:Forensic Genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D007202:Indicators and Reagents