Reversed-phase liquid chromatography on a microchip with sample injector and monolithic silica column.
In micro total analysis systems, liquid chromatography (LC) works under pressure-driven flow is the essential analysis component. There were not, however, much works on microchip LC. Here we developed a microchip for reversed-phase LC using porous monolithic silica. The chip consisted of a double T-shaped injector and a approximately 40-cm serpentine separation channel. The octadecyl-modified monolithic silica was prepared in the specified part of the channel on the microchip using sol-gel process. Furthermore, the effect of geometry of turn sections on band dispersion at turns was examined under pressure-driven flow. High separation efficiencies of 15,000-18,000 plates/m for catechins were obtained using the LC chip.
Ishida
Akihiko
A
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Nishi 8, Kita 13, Sapporo 060-8628, Japan. ishida-a@eng.hokudai.ac.jp <ishida-a@eng.hokudai.ac.jp>
Yoshikawa
Takahiro
T
Natsume
Masamichi
M
Kamidate
Tamio
T
D002392:Catechin / Q000032:analysis / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012822:Silicon Dioxide
D012822:Silicon Dioxide; D002392:Catechin