Ovine progressive pneumonia virus capsid is B-cell immunodominant using Western blot analysis: a comparison of sensitivity between Western blot analysis and immunoprecipitation.
A Western blot assay was developed and analyzed against the comparable standard, immunoprecipitation of (35)[S]-methionine/cysteine-labeled ovine progressive pneumonia virus (OPPV) proteins, for its ability to detect anti-OPPV antibodies using endpoint titers. Western blot analysis is 12-fold more sensitive in detecting endpoint anti-capsid antibody titers than IP, and the capsid is the B-cell immunodominant OPPV protein when utilizing Western blot analysis. Since the surface envelope glycoprotein is the B-cell immunodominant OPPV protein when utilizing immunoprecipitation, this suggests immunoprecipitation and Western blot analysis measure different types of antibody that are more specific for conformational and linear OPPV protein epitopes, respectively.
Myers-Evert
Dawn K
DK
Washington State University, College of Veterinary Medicine, Pullman, WA 99164, USA. dkevert@vetmed.wsu.edu
Herrmann-Hoesing
Lynn M
LM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D015153:Blotting, Western; D002213:Capsid / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D016056:Immunodominant Epitopes / Q000276:immunology*; D047468:Immunoprecipitation; D016086:Lentivirus / Q000276:immunology*; D016180:Lentivirus Infections / Q000175:diagnosis / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis
D000914:Antibodies, Viral; D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D016056:Immunodominant Epitopes