title: Hypoxia-inducible factor prolyl hydroxylase 2 has a high affinity for ferrous iron and 2-oxoglutarate.
abstract: Regulation of the hypoxic response in humans is regulated by the post-translational hydroxylation of hypoxia inducible transcription factor; a recombinant form of a human prolyl-4-hydroxylase (PHD2) was characterised and shown to have an unexpectedly high affinity for, and to copurify with endogenous levels of, its Fe(ii) cofactor and 2-oxoglutarate cosubstrate.
authors.affiliation: The Department of Chemistry and The Oxford Centre for Molecular Sciences, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, UK.
authors.forename: Luke A
authors.initials: LA
authors.lastname: McNeill
authors.forename: Emily
authors.initials: E
authors.lastname: Flashman
authors.forename: Matthew R G
authors.initials: MR
authors.lastname: Buck
authors.forename: Kirsty S
authors.initials: KS
authors.lastname: Hewitson
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Clifton
authors.forename: Gunnar
authors.initials: G
authors.lastname: Jeschke
authors.forename: Timothy D W
authors.initials: TD
authors.lastname: Claridge
authors.forename: Dominic
authors.initials: D
authors.lastname: Ehrismann
authors.forename: Neil J
authors.initials: NJ
authors.lastname: Oldham
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Schofield
mesh_terms: D001665:Binding Sites; D002853:Chromatography, Liquid; D005296:Ferrous Compounds / Q000737:chemistry*; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000737:chemistry*; D007656:Ketoglutaric Acids / Q000737:chemistry*; D013058:Mass Spectrometry; D008958:Models, Molecular; D011393:Procollagen-Proline Dioxygenase / Q000737:chemistry*
chemical_list: D005296:Ferrous Compounds; D051793:Hypoxia-Inducible Factor 1; D007656:Ketoglutaric Acids; D011393:Procollagen-Proline Dioxygenase