SNAP-25 in hippocampal CA3 region is required for long-term memory formation.
SNAP-25 is a synaptosomal protein of 25 kDa, a key component of synaptic vesicle-docking/fusion machinery, and plays a critical role in exocytosis and neurotransmitter release. We previously reported that SNAP-25 in the hippocampal CA1 region is involved in consolidation of contextual fear memory and water-maze spatial memory (Hou et al. European J Neuroscience, 20: 1593-1603, 2004). SNAP-25 is expressed not only in the CA1 region, but also in the CA3 region, and the SNAP-25 mRNA level in the CA3 region is higher than in the CA1 region. Here, we provide evidence that SNAP-25 in the CA3 region is also involved in learning/memory. Intra-CA3 infusion of SNAP-25 antisense oligonucleotide impaired both long-term contextual fear memory and water-maze spatial memory, with short-term memory intact. Furthermore, the SNAP-25 antisense oligonucleotide suppressed the long-term potentiation (LTP) of field excitatory post-synaptic potential (fEPSP) in the mossy-fiber pathway (DG-CA3 pathway), with no effect on paired-pulse facilitation of the fEPSP. These results are consistent with the notion that SNAP-25 in the hippocampal CA3 region is required for long-term memory formation.
Hou
Qiu-Ling
QL
Laboratory of Higher Brain Functions, Institute of Neurobiology, Institutes of Brain Science, Fudan University, 220 Han-Dan Road, Shanghai 200433, China.
Gao
Xiang
X
Lu
Qi
Q
Zhang
Xue-Han
XH
Tu
Yan-Yang
YY
Jin
Mei-Lei
ML
Zhao
Guo-Ping
GP
Yu
Lei
L
Jing
Nai-He
NH
Li
Bao-Ming
BM
D000818:Animals; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology; D005239:Fear / Q000502:physiology; D006624:Hippocampus / Q000033:anatomy & histology* / Q000187:drug effects; D017774:Long-Term Potentiation / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D008568:Memory / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013037:Spatial Behavior / Q000187:drug effects / Q000528:radiation effects; D050825:Synaptosomal-Associated Protein 25 / Q000502:physiology*
D016376:Oligonucleotides, Antisense; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25