Compact polytetrafluoroethylene assembly-type capillary electrophoresis with chemiluminescence detection.
We have developed a compact polytetrafluoroethylene (PTFE) assembly-type capillary electrophoresis with chemiluminescence (CL) detection system. Luminol-microperoxidase-hydrogen peroxide chemiluminescence reaction was adopted. The device is rectangular in shape (60 mm x 40 mm x 30 mm) and includes three reservoirs (sample, migration buffer, and detection reservoirs) with electrodes. The detection reservoir includes an optical fiber to transport light at the capillary tip to a photomultiplier tube. Isoluminol isothiocyanate (ILITC) was analyzed as a model using this device with fused-silica or polytetrafluoroethylene capillary tubes 10 cm in length. We also used the sample reservoir as a reactor for an immune reaction between anti-human serum albumin immobilized on glass beads and isoluminol isothiocyanate-labeled human serum albumin. The present polytetrafluoroethylene assembly with the capillary tube was useful as a palm-sized analysis device for separation and detection, as well as a reactor.
Tsukagoshi
Kazuhiko
K
Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Kyotanabe 610-0321, Japan. ktsukago@mail.doshisha.ac.jp
Ishida
Shingo
S
Oda
Yuichi
Y
Noda
Keiichi
K
Nakajima
Riichiro
R
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry
D012822:Silicon Dioxide; D011138:Polytetrafluoroethylene