Diethanolamine alters neurogenesis and induces apoptosis in fetal mouse hippocampus.
Diethanolamine (DEA) is present in many consumer products such as shampoo. Dermal administration of DEA diminishes hepatic stores of the essential nutrient choline, and we previously reported that dietary choline deficiency during pregnancy reduces neurogenesis and increases apoptosis in the hippocampus of fetal rats and mice. Therefore, DEA could also alter brain development. Timed-pregnant C57BL/6 mice were dosed dermally from gestation day 7 through 17 with DEA at 0, 20, 80, 160, 320, and 640 mg/kg body/day. At doses of DEA > 80 mg/kg body/day, we observed decreased litter size. In fetuses (embryonic day 17) collected from dams treated dermally with 80 mg/kg body/day DEA, we observed decreased neural progenitor cell mitosis at the ventricular surface of the ventricular zone of the hippocampus [to 56+/-14% (se) histone 3 (H3) phosphorylation as compared to controls; P < 0.01]. We also observed increased apoptosis in fetal hippocampus (to 170+/-10% of control measured using TUNEL and to 178+/-7% of control measured using activated caspase 3; P < 0.01). Thus, maternal exposure to DEA reduces the number of neural progenitor cells in hippocampus by two mechanisms, and this could permanently alter memory function in offspring of mothers exposed to this common ingredient of shampoos and soaps.
Craciunescu
Corneliu N
CN
Department of Nutrition, School of Public Health and Medicine, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina 27599-7461, USA.
Wu
Renan
R
Zeisel
Steven H
SH
D000287:Administration, Topical; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004983:Ethanolamines / Q000009:adverse effects*; D005260:Female; D005333:Fetus; D006624:Hippocampus / Q000166:cytology* / Q000254:growth & development; D008098:Litter Size; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects
D004983:Ethanolamines; C020283:diethanolamine