Genetically engineered poly-gamma-glutamate producer from Bacillus subtilis ISW1214.
The pgsBCA-gene disruptant from Bacillus subtilis ISW1214, i.e., MA41, does not produce poly-gamma-glutamate (PGA). We newly constructed an MA41 recombinant bearing the plasmid-borne PGA synthetic system, in which PGA production was strictly controlled by the use of xylose. Unlike the parent strain, ISW1214, the genetically engineered strain produced abundant PGA in both L-glutamate-rich and D-glutamate-rich media.
Ashiuchi
Makoto
M
Department of Bioresources Science, Kochi University, Kochi 783-8502, Japan. ashiuchi@cc.kochi-u.ac.jp
Shimanouchi
Kazuya
K
Horiuchi
Terumi
T
Kamei
Tohru
T
Misono
Haruo
H
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005818:Genetic Engineering; D018698:Glutamic Acid / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D013237:Stereoisomerism; D014994:Xylose / Q000378:metabolism
C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D018698:Glutamic Acid; D014994:Xylose