Stereospecific high-performance liquid chromatographic analysis of eriodictyol in urine.
A stereospecific method of analysis of eriodictyol [5,7,3',4'-tetrahydroxyflavanone] in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism, and tissue distribution in fruits and humans. A simple high-performance liquid chromatographic method was developed for the stereospecific determination of eriodictyol in rat and human urine. Separation was achieved on a Chiralpak OJ-RH column with UV detection at 288 nm. The stereospecific calibration curves were linear ranging from 0.5 to 100 microg/ml. The mean extraction efficiency was >98.8%. Precision of the assay was <15% (CV), and was within 12% at the limit of quantitation (0.5 microg/ml). Bias of the assay was lower than 8%, and was within 6% at the limit of quantitation. The assay was applied successfully to the urinary excretion of eriodictyol in rats and humans, and to the stereospecific quantification of eriodictyol in raw lemon juice, conventional and organic lemonade.
Yáñez
Jaime A
JA
College of Pharmacy, Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program, Washington State University, Pullman, WA 99164-6534, USA.
Miranda
Nicole D
ND
Remsberg
Connie M
CM
Ohgami
Yusuke
Y
Davies
Neal M
NM
D000818:Animals; D001628:Beverages / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D044950:Flavanones / Q000652:urine*; D005615:Freezing; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D013237:Stereoisomerism
D044950:Flavanones; D007202:Indicators and Reagents; C007619:eriodictyol