Partial two-dimensional gel electrophoresis (2-DE) maps of Streptococcus iniae ATCC29178 and Lactococcus garvieae KG9408.
To examine the proteomes of 2 important causative agents of fish streptococcosis, Streptococcus iniae ATCC29178 and Lactococcus garvieae KG9408, we used 2-dimensional gel electrophoresis (2-DE) followed by mass spectrometry to generate 2-DE maps of these type strains. Silver-stained 2-DE gels of S. iniae ATCC29178 and L. garvieae KG9408 revealed approximately 320 and 300 spots, respectively, and immobilized pH gradient strips (13 cm, pH 4 to 7) revealed that the majority of the detected spots were concentrated in the pH range of 4.5 to 5.5. The spots were randomly selected from the 2-DE profiles and identified by peptide mass fingerprinting using matrix-assisted laser desorption/ionization time of flight mass spectrometry. The majority of the identified proteins were functionally related to energy and carbohydrate metabolism (e.g. enolase ATPase, glyceraldehyde-3-phosphate dehydrogenase) or translation and translocation (e.g. elongation factor G, elongation factor Tu, DNA-directed RNA polymerase alpha chain). These data, along with our partial 2-DE maps of S. iniae ATCC29178 and L. garvieae KG9408, may help suggest antigenic proteins for the development of effective diagnostic tools and vaccines against S. iniae and L. garvieae.
Shin
Gee-Wook
GW
Laboratory of Fish & Shellfish Diseases, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, ROK.
Palaksha
K J
KJ
Yang
Hyang-Hee
HH
Shin
Yong-Seung
YS
Kim
Young-Rim
YR
Lee
Eun-Young
EY
Oh
Myung-Joo
MJ
Jung
Tae-Sung
TS
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000145:classification*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005399:Fishes; D017045:Lactococcus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D020543:Proteome / Q000235:genetics*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013291:Streptococcus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
D001426:Bacterial Proteins; D020543:Proteome