Mixed monolayer-protected gold nanoclusters as selective peptide extraction agents for MALDI-MS analysis.
Cationic and anionic nanoparticles selectively target peptides with low and high isoelectric points, respectively. Additionally, their high surface area-to-volume ratios make these nanoparticles (approximately 2-nm core diameter) very efficient extraction and concentration agents. Upon extraction, the peptide-bound nanoparticles can be analyzed by MALDI-MS to provide highly sensitive detection of the targeted peptides. We demonstrate that MALDI-MS can detect peptide concentrations as low as 500 pM from 250-microL solutions using these nanoparticle scaffolds as extraction and concentration agents.
Vanderpuije
Benjamin N Y
BN
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Han
Gang
G
Rotello
Vincent M
VM
Vachet
Richard W
RW
D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D008567:Membranes, Artificial; D010455:Peptides; D006046:Gold