title: [Effects of T cell receptor gene rearrangement on BV CDR3 in Jurkat cells].
abstract: OBJECTIVE
  To investigate the effects of T cell receptor (TCR) BD2-BJ2 gene rearrangement on the complementary-determining region (CDR) 3 of TCR beta chain (TCR BV CDR3) in Jurkat cells.
  
  
  METHODS
  TCR BV gene subfamilies were detected by RT-PCR in Jurkat cells during proliferation and after induction with non-specific T cell activators and SEA, respectively. To determine the clonality of TCR BV subfamilies and the lengths of CDR3, the PCR products were analyzed by TCR GeneScan technique, and the sequences of CDR3 were further analyzed by DNA sequencer.
  
  
  RESULTS
  No new TCR BV subfamilies were found in Jurkat cells, a monoclonal BV8(+)cell line, either during cell proliferation or after stimulation with different treatments, nor were any differences found in CDR3 size or sequences.
  
  
  CONCLUSIONS
  TCR BD2-BJ2 rearrangement in Jurkat cells may not play a role in modification of TCR BV CDR3 domains or the consequent antigen immunorecognition of BV CDR3, but the possibility of TCR modification can not be excluded.
authors.affiliation: Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Zou
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Xin-sheng
authors.initials: XS
authors.lastname: Yao
authors.forename: Qian
authors.initials: Q
authors.lastname: Wen
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D022801:Complementarity Determining Regions / Q000235:genetics*; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics*; D006801:Humans; D007155:Immunologic Factors / Q000235:genetics; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
chemical_list: D022801:Complementarity Determining Regions; D007155:Immunologic Factors; D011948:Receptors, Antigen, T-Cell