title: A new triterpene and anti-hepatitis B virus active compounds from Alisma orientalis.
abstract: A new triterpenoid named alisol O ( 1) was isolated from the rhizomes of Alisma orientalis, together with six known compounds: alisol A 24-acetate ( 2), 25-anhydroalisol A ( 3), 13 beta,17 beta-epoxyalisol A ( 4), alisol B 23-acetate ( 5), alisol F ( 6), and alisol F 24-acetate ( 7). Based on 1D and 2D-NMR data (HMQC, HMBC, COSY, ROESY), the structure of the new compound was deduced to be 11-dehydroxy-12-dehydroalisol F-24-acetate ( 1). Compounds 2 - 7 exhibited inhibitory activity in vitro on hepatitis B virus (HBV) surface antigen (HBsAg) secretion of the Hep G2.2.15 cell line with IC (50) values of 2.3, 11.0, 15.4, 14.3, 0.6 and 7.7 microM, and on HBV e antigen (HBeAg) secretion with IC (50) values of 498.1, 17.6, 41.0, 19.9, 8.5 and 5.1 microM, respectively.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, The Chinese Academy of Sciences, Kunming, Yunnan, PR China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Jiang
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Feng-Xue
authors.initials: FX
authors.lastname: Zhang
authors.forename: Ni
authors.initials: N
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Ji-Jun
authors.initials: JJ
authors.lastname: Chen
mesh_terms: D034902:Alisma / Q000737:chemistry*; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: C515797:24-hydroxyprotosta-11,13-diene 24-acetate; D000998:Antiviral Agents; D010936:Plant Extracts; D014315:Triterpenes