Detection and transcript expression of S-RNase gene associated with self-incompatibility in apricot (Prunus armeniaca L.).
The identity and expression of S-RNase genotypes in the self-compatible (SC) apricot cultivar 'Katy' and the self-incompatible (SI) cultivar 'Xinshiji' were examined. We used allele specific polymerase chain reaction (AS-PCR) and designated the alleles in 'Katy' and 'Xinshiji' as S(8)Sc and S(9)S(10), respectively. The S-RNase gene was expressed in style at the balloon stage in both genotypes. Using real-time fluorescence quantification RT-PCR technology (FQRT-PCR), spatio-temporal expression patterns of S-RNase gene between 'Katy' and 'Xinshiji' were compared. The results revealed that the expression of the S-RNase gene in 'Katy' and 'Xinshiji' were different. The transcript abundance was distinctly diverse at the key stage (i.e., at 24 h after self-pollination) in both genotypes, and was greater in 'Xinshiji' (SI) than 'Katy' (SC). In addition, the abundance of the S-RNase transcript was higher in upper-half of style than in the lower-half of style or in the ovary. In the SI cultivar 'Xinshiji', the expression of S-RNase reminded a relatively high level after cross-pollination, but it dropped continuously after self-pollination and un-pollination.
Feng
Jianrong
J
Horticultural Science and Engineering College, Shandong Agricultural University, Daizong Street 61, 271018 Taian, Shandong, China.
Chen
Xuesen
X
Wu
Yan
Y
Liu
Wen
W
Liang
Qing
Q
Zhang
Lijie
L
D000483:Alleles; D035264:Flowers / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D016133:Polymerase Chain Reaction; D027861:Prunus / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D012260:Ribonucleases / Q000235:genetics*
D012260:Ribonucleases