title: [Determination of artemisinin in Artemisia annuna by HPLC-ELSD].
abstract: OBJECTIVE
  A HPLC method was first established to determine artemisinin in Artemisia annuna by HPLC-ELSD. Artemisinin in Herba of Artemisia annuna from different places was determined by this new method.
  
  
  METHODS
  The method utilized MeOH-H2O (75: 25) as mobile phase with flow rate of 1 ml/min, C18 (250 x 4.6 mm, 5 microm) column. The temperature of detector was 40 degrees C, the pressure of N2 was 3.5 bar, and gain value was 9.
  
  
  RESULTS
  The calibration curves were linear within the range of 1 - 5 microg, and the average recovery was 99.33% (RSD = 1.97%).
  
  
  CONCLUSIONS
  The method was rapid, convenient, and accurate. It can be used for the quality control of this herbal medicine.
authors.affiliation: National Engineering Research Center for Modernization of Extraction and Separation Process of TCM, Guangzhou 510240, China.
authors.forename: Ao-Ao
authors.initials: AA
authors.lastname: Zhou
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Zheng
authors.forename: Fa-Huan
authors.initials: FH
authors.lastname: Ge
mesh_terms: D000962:Antimalarials / Q000737:chemistry; D031172:Artemisia annua / Q000737:chemistry* / Q000145:classification; D037621:Artemisinins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012621:Seasons; D012717:Sesquiterpenes / Q000032:analysis*
chemical_list: D000962:Antimalarials; D037621:Artemisinins; D004365:Drugs, Chinese Herbal; D012717:Sesquiterpenes; C031327:artemisinin