title: Outer membrane protein changes and efflux pump expression together may confer resistance to ertapenem in Enterobacter cloacae.
abstract: We investigated ertapenem-susceptible and -resistant extended-spectrum beta-lactamase-producing Enterobacter cloacae isolates obtained from the same patient. Gene transcription of OmpD and OmpF was diminished in the ertapenem-resistant isolate. An efflux pump inhibitor decreased the MICs of ertapenem in the resistant strain, suggesting a potential role of efflux pumps in ertapenem resistance.
authors.affiliation: Division of Infectious Diseases, University of Pittsburgh Medical Center, PA 15213, USA.
authors.forename: Dóra
authors.initials: D
authors.lastname: Szabó
authors.forename: Fernanda
authors.initials: F
authors.lastname: Silveira
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Hujer
authors.forename: Robert A
authors.initials: RA
authors.lastname: Bonomo
authors.forename: Kristine M
authors.initials: KM
authors.lastname: Hujer
authors.forename: Jane W
authors.initials: JW
authors.lastname: Marsh
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Bethel
authors.forename: Yohei
authors.initials: Y
authors.lastname: Doi
authors.forename: Kathleen
authors.initials: K
authors.lastname: Deeley
authors.forename: David L
authors.initials: DL
authors.lastname: Paterson
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D016972:Enterobacter cloacae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D000077727:Ertapenem; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D014158:Transcription, Genetic; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D026901:Membrane Transport Proteins; D047090:beta-Lactams; D000077727:Ertapenem