[Haplotypes of DXS10011 and DXS8377 in Guangdong Han individuals].
OBJECTIVE
To investigate genetic polymorphism of two X chromosome specific STR: DXS10011 and DXS8377 in male samples from Guangdong Han population.


METHODS
The DNA samples were amplified by PCR and analyzed by polyacrylamide gel electrophoresis followed by silver staining.


RESULTS
Among 113 samples, 20 alleles were found for DXS1011 and 12 alleles for DXS8377. Also, 72 DXS10011-DXS8377 haplotypes were shown. The most common haplotypes only occurred three times. When only female children were tested in motherless case, the exclusion probability of paternity was 0.9588 for DXS10011-DXS8377 haplotypes. Investigations in 83 family trios with female children and 29 pedigrees with two children suggested a co-dominant X-linked inheritance; mutations were not found.


CONCLUSIONS
Our data indicate that DXS10011 and DXS8377 are highly informative X chromosome markers for complicated kinship analysis.
Lu
De-Jian
DJ
Department of Forensic Serology, Zhongshan-Medical College, Sun Yat-sen University, Guangzhou 510089, China. dejian6182@sina.com
Chen
Shu-Chun
SC
Wang
Xue-Wen
XW
Liu
Qiu-Ling
QL
Lu
Hui-Ling
HL
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China; D041321:Chromosomes, Human, X / Q000235:genetics*; D004247:DNA / Q000097:blood / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D044707:Forensic Sciences; D005787:Gene Frequency; D008040:Genetic Linkage; D006239:Haplotypes; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
D004247:DNA