Use of microcoil probehead for determination of the structure of oriented silk fibers by solid-state NMR.
A microcoil probehead for solid-state NMR was developed with a two-channel radio-frequency circuit, and 13C observation with a proton-decoupling probehead was performed to obtain information on the distribution of the orientation of silk fibroin molecules in the fiber. The coil (1 mm (diameter) x 5 mm (length)) of the probehead was placed at the angles 90 degrees and 30 degrees , relative to the static magnetic field. Only 70 mug of [1-13C]Gly silk fibroin fiber was used in a magnet of 9.4 T (400 MHz for proton channel).
Yamauchi
K
K
Department of Biotechnology, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Koganei, Tokyo 184-8588, Japan.
Imada
T
T
Asakura
T
T
D000409:Alanine / Q000737:chemistry; D004574:Electromagnetic Fields; D005349:Fibroins / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods; D047011:Silk / Q000737:chemistry*
D047011:Silk; D005349:Fibroins; D000409:Alanine; D005998:Glycine