title: The ADP/ATP ratio: A novel predictive assay for quality assessment of isolated pancreatic islets.
abstract: The current standard assays for islet product release criteria are unable to predict the outcome after clinical islet transplantation. Therefore, establishment of reliable and rapid assays enabling pre-transplantation prediction of islet potency is warranted. In the present study, we have evaluated the adenosine diphosphate (ADP)/adenosine triphosphate (ATP) test, the glucose-stimulated insulin release, the loss of islets during the first 24 h in culture, and the insulin/deoxyribonucleic acid as predictive assays for the ability of isolated porcine islets to cure athymic mice with streptozotocin-induced diabetes. From the results presented, it is concluded that the measurement of the ADP/ATP ratio was the only test that correlated with transplantation outcome. In summary, we propose that the ADP/ATP assay is worthwhile as applied to human islet transplantation and seek to validate it as a rapid and potent predictor of transplant outcome.
authors.affiliation: Tohoku University, Biomedical Engineering Research Organization, Sendai, Japan. gotpkichi@aol.com
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.forename: J
authors.initials: J
authors.lastname: Holgersson
authors.forename: M
authors.initials: M
authors.lastname: Kumagai-Braesch
authors.forename: O
authors.initials: O
authors.lastname: Korsgren
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D006801:Humans; D006943:Hyperglycemia / Q000175:diagnosis / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011183:Postoperative Complications; D011379:Prognosis; D013552:Swine
chemical_list: D015415:Biomarkers; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate