[The relationship between histone acetylation modification and IFN-gamma responsive gene regulation].
OBJECTIVE
To study the role of histone modification in the regulation of IFN-gamma-activated gene using chromatin immunoprecipitation technique.


METHODS
Real-time PCR was used to measure the mRNA level of interferon-gamma-inducible protein 10 (IP-10) in Hela cells. Chromatin immunoprecipitation combined with Real-time PCR was used to check the histone H4 acetylation level at IFN-stimulated response element (ISRE) locus of IP-10 gene.


RESULTS
IP-10 was strongly activated by IFN-gamma. The histone H4 deacetylation happened at the ISRE locus when IP-10 was induced by IFN-gamma. The activation of IP-10 and the deacetylation of histone H4 at the ISRE site induced by IFN-gamma were inhibited or blocked by histone deacetylase (HDAC) inhibitor trichostatin A (TSA).


CONCLUSIONS
The histone H4 deacetylation at the ISRE site is related with the activation of IP-10 by IFN-gamma.
Guo
Jin-jun
JJ
Key Laboratory of Molecular Biology of Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Huang
Ying
Y
Zhang
Jun
J
Huang
Ai-long
AL
D000107:Acetylation; D054357:Chemokine CXCL10 / Q000378:metabolism*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D006657:Histones; D012333:RNA, Messenger; D007371:Interferon-gamma; D006655:Histone Deacetylases