Application of DNA markers to estimate genetic diversity of Mycobacterium tuberculosis strains.
The obligatory human pathogen, Mycobacterium tuberculosis, is the most important etiological factor of tuberculosis. Unfortunately, there is little information about genetic diversity of this pathogen. The main aim of this research was the estimation of genetic diversity of M. tuberculosis on the basis of various categories of DNA markers. The genome of 32 strains were scanned by DNA markers such RAPD, IS6110 and catalase-peroxidase katG gene. All 162 identified loci were polymorphic. The genetic diversity coefficient (HT) of M. tuberculosis was 0.32 for RAPD and 0.27 for IS 6110. There were 14 alleles in katG gene. All strains were characterised by the individual molecular pattern. Genetic similarity varied from 0.13 to 0.94 (RAPD markers) and from 0 to 1 for (IS6110). M. tuberculosis strains did not represent a clonal structure, single source of transmission and epidemiological relationships as well. The applied DNA markers proved to be highly efficient for analysis of genetic structure of M. tuberculosis.
Korzekwa
Karol
K
Department of Environmental Microbiology, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury in Olsztyn, Prawocheńskiego street 1, 10-720 Olsztyn. idefix@moskit.uwm.edu.pl
Polok
Kornelia
K
Zieliński
Roman
R
D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
D005819:Genetic Markers