[Determination of human serum glutathione peroxidase activity, by using hydrogen peroxide and 5,5'-dithio-bis(2-nitrobenzoic acid)].
The method has been developed for the assay of human serum glutathione:H2O2-oxidoreductase activity. The reaction medium comprises tris-HCl buffer (0.04 M; pH 8.5), 4.4 mM EDTA, 10 mM NaN3, 1 mM GSH, 400 microM Y2O2. The incubation time of 60 seconds at 37 degrees C was chosen. There was a decrease in GSH in the reaction with 5,5'-dithio-bis(2-nitrobenzoic acid). This method is recommended for the application to the assay of selenium-dependent glutathione peroxidase because hydrogen peroxide (H2O2) used in this method is a more specific substrate than commonly used organic hydroperoxides.
Razygraev
A V
AV
Arutiunian
A V
AV
D004228:Dithionitrobenzoic Acid / Q000737:chemistry*; D005979:Glutathione Peroxidase / Q000097:blood* / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000134:cerebrospinal fluid* / Q000737:chemistry*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D004228:Dithionitrobenzoic Acid; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase