title: [An epidemiological investigation of bats carrying SARS-CoV in Guangzhou and its vicinity].
abstract: OBJECTIVE
  To detect serve acute respiratory syndrome-associated coronavirus (SARS-CoV) and SARS-like-CoV in fruit bats captured in Guangzhou and its vicinity.
  
  
  METHODS
  Totally 927 bats of 9 species (Cynopterus sphinx, Rousettus leschenaulti, Miniopterus schreibersi, Hipposideros pratti, Rhinolophusasinicus, Scotophilusakuhlii, Hipposideros Pomona, Rhinolophus affinis, and Rhinolophus pusillus) captured in Guangzhou and its vicinity from September 2004 to November 2005 were available for this investigation, from which 3,043 samples (813 throat swasb, 524 sera, 853 lung tissues and 853 colorectal tissue specimens) were obtained. SARS-Cov and SARS-like-CoV were detected in these specimens using diagnostic kit for novel coronavirus N protein (ELISA), SARS-CoV Virus RNA detection kit, fluorescence PCR, Genchip, RT-PCR and cell isolation culture methods.
  
  
  CONCLUSIONS
  No SARS-CoV and SARS-like-CoV were detected in the 3043 samples, indicating the current absence of SARS-CoV and SARS-like-CoV in the bats captured in Guangzhou and its vicinity.
authors.affiliation: Department of Epidemiology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.forename: Hui-chun
authors.initials: HC
authors.lastname: Zhan
authors.forename: Xue-xia
authors.initials: XX
authors.lastname: Yun
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Du
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Ke
authors.forename: De-xian
authors.initials: DX
authors.lastname: Yu
authors.forename: Jian-dong
authors.initials: JD
authors.lastname: Li
authors.forename: Ying-chun
authors.initials: YC
authors.lastname: Dai
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.forename: Shou-yi
authors.initials: SY
authors.lastname: Yu
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D002685:Chiroptera / Q000821:virology*; D000086462:Coronavirus Nucleocapsid Proteins; D004199:Disease Vectors; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019590:Nucleocapsid Proteins / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D045169:Severe Acute Respiratory Syndrome / Q000453:epidemiology / Q000635:transmission / Q000821:virology
chemical_list: D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D012367:RNA, Viral