Evaluation of enterovirus recovery in surface water by different adsorption and elution procedures.
The performance of six concentration method combinations and two quantitative analysis techniques were evaluated in terms of enterovirus recovery efficiencies by adjusting the pH and salt concentration of water samples. Of the six concentration method combinations, adsorption on nitrocellulose membranes followed by an acid rinse elution consistently gave the highest recovery efficiencies. In theory, an electropositive membrane should be the most appropriate technique for adsorption of electronegative viruses in pure water. However, it displayed the greatest loss in natural waters. For adsorption and elution procedures, both the electronegative membrane, accompanied by an acid rinse step, and the electropositive membrane, accompanied by a glycine rinse step, provided higher recovery efficiencies. MPN-RT-PCR, a statistically quantitative analysis method, performed more efficiently, in economic terms, but had a similar enterovirus recovery trend to real-time RT-PCR, which is the authoritative quantitative analysis method for nucleic acid.
Hsu
Bing-Mu
BM
Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan.
Chen
Chien-Hsien
CH
Kung
Chien-Min
CM
Wan
Min-Tao
MT
Shen
Shu-Min
SM
D000327:Adsorption; D003101:Collodion / Q000737:chemistry; D004563:Electrochemistry; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004784:Environmental Monitoring; D005374:Filtration; D006863:Hydrogen-Ion Concentration; D008851:Micropore Filters; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014871:Water Microbiology; D014873:Water Pollutants / Q000302:isolation & purification*
D012367:RNA, Viral; D014873:Water Pollutants; D003101:Collodion