Chromosome analysis of uncultured amniocytes by comparative genomic hybridization in early amniocentesis.
OBJECTIVE
To study chromosome analysis by comparative genomic hybridization (CGH) compared with the conventional technique in early amniocentesis.


METHODS
Cross-sectional descriptive study design was performed in 32 singleton pregnant women with gestational age between 12-15 weeks. Transabdominal amniocentesis was carried out under ultrasound guidance. The amniotic fluid samples were simultaneously investigated using CGH and the conventional cytogenetics study as a gold standard.


RESULTS
Amniocentesis were done for advanced maternal age in all cases. The mean maternal age was 35.8 years (35-42 years). The mean gestational age was 13.7 weeks (12-15 weeks). The chromosome analysis by CGH technique of uncultured amniocyte showed 17 normal female chromosomes (53.1%) and 15 normal male chromosomes (46.9%). This finding was the same as the conventional cytogenetics method. The mean duration of the CGH method was 6 days and that of the conventional cytogenetics method was 13.7 days (10-23 days).


CONCLUSIONS
The CGH technique is a reliable technique for a rapid prenatal diagnosis of chromosome study in early gestation.
Ketupanya
Aphornpirom
A
Department of Obstetrics, Phramongkutklao Hospital, Bangkok, Thailand. chrodna@yahoo.com
Aranyakasemsuk
Niwat
N
Tocharoentanaphol
Chintana
C
Vuthiwong
Chutawadi
C
D000328:Adult; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology*; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008297:Male; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D015203:Reproducibility of Results