title: Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown.
abstract: A number of natural microRNA (miRNA) hairpins have been found in clusters of multiple identical or different copies, suggesting that effects of miRNAs can be enhanced and multiple genes can be regulated together by encoding multiple miRNA hairpins in a single transcript. Here, we report a simple and effective artificial multi-hairpin method that stimulates production of mature 22-nucleotide small RNAs from modified miRNA hairpins, improves gene knockdown over single-hairpin constructs, and provides linked multi-gene knockdowns.
authors.affiliation: Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Daqian
authors.initials: D
authors.lastname: Sun
authors.forename: Margherita
authors.initials: M
authors.lastname: Melegari
authors.forename: Sunandini
authors.initials: S
authors.lastname: Sridhar
authors.forename: Charles E
authors.initials: CE
authors.lastname: Rogler
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D045744:Cell Line, Tumor; D005821:Genetic Techniques; D005822:Genetic Vectors; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D010957:Plasmids / Q000378:metabolism; D012313:RNA / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D009841:Oligonucleotides; D012313:RNA