title: [Overexpression of PEMT2 inhibits the phosphorylation and translocation of PLC gamma 1].
abstract: OBJECTIVE
  To explore the mechanism of CBRH-7919 cell proliferation inhibition by transfecting phosphatidylethanolamine N-methyltransferase 2 gene (PEMT2).
  
  
  METHODS
  The effects of PEMT2 transfection on phosphorylation and translocation from cytosol to plasma membrane of PLC gamma 1 in cells were studied using SDS-PAGE and Western blot techniques. The phosphorylation and activity of c-Met were determined.
  
  
  RESULTS
  After transfection of pemt2, the PLC gamma 1 and phosphorylated PLC gamma 1 conjugated with plasma membrane were decreased by 45% and 27% of that of control cells respectively, and the phosphorylated c-Met was decreased to 32% of that of control cells.
  
  
  CONCLUSIONS
  Transfection of phosphatidylethanolamine N-methyltransferase 2 gene can inhibit the phosphorylation and translocation from cytosol to plasma membrane of PLC gamma 1 in cells. At the same time, the autophosphorylation of c-Met was decreased, which suggests that transfection of phosphatidylethanolamine N-methyltransferase 2 gene can downregulate the c-Met/PLC gamma 1 signaling pathway in CBRH-7919 cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116027, China.
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Li
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Shao
authors.forename: Zhi-li
authors.initials: ZL
authors.lastname: Liu
authors.forename: Quan
authors.initials: Q
authors.lastname: Xia
authors.forename: Ke-li
authors.initials: KL
authors.lastname: Ma
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D008114:Liver Neoplasms, Experimental; D050918:Phosphatidylethanolamine N-Methyltransferase / Q000235:genetics* / Q000378:metabolism; D051966:Phospholipase C gamma / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D051381:Rats; D014162:Transfection
chemical_list: D050918:Phosphatidylethanolamine N-Methyltransferase; D019859:Proto-Oncogene Proteins c-met; D051966:Phospholipase C gamma