title: VapI, a new member of the Rhodococcus equi Vap family.
abstract: Rhodococcus equi is a facultative intracellular bacterium which can cause bronchopneumonia in foals and AIDS patients. In this report we show that the ORF13-protein coded by the virulence associated plasmid of R. equi is clearly homologous to VapE. Nucleotide sequence analysis revealed frame shift mutations that shorten the sequence of the ORF13-protein. A theoretical extension of the sequence of ORF13 by the introduction of a single nucleotide yields a translated amino acid sequence that is highly homologous to VapE and other members of the Vap family. The data provided in this study indicate that the ORF13-protein is a novel member of the Vap family and is therefore designated VapI.
authors.affiliation: Institut für Zellbiologie and Bonner Forum Biomedizin, University of Bonn, Ulrich-Haberland-Str. 61a, 53121, Bonn, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Polidori
authors.forename: Albert
authors.initials: A
authors.lastname: Haas
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D016929:Rhodococcus equi / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins