[Recombinant human endostatin induces apoptosis of human umbilical venous endothelial cells].
To investigate the effects of human endostatin on proliferation of human umbilical venous endothelial cells, the apoptosis of the endothelial cells treated with recombinant human endostatin was examined by fluorescence method and the cell proliferation activity analyzed by MTT assay. The results showed that human endostatin inhibited the proliferation of the endothelial cells with ED(50) of 550 ng/ml, and the inhibitory effect was enhanced with the increase of endostatin concentration, suggesting a dose-dependent inhibitory effect of human endostatin on endothelial cell proliferation. The mechanism of antiangiogenesis induced by human endostatin is related to the inhibition of cell proliferation and induction of apoptosis of the endothelial cells.
Yang
Fang
F
Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
He
Yuan-li
YL
D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D043169:Endostatins / Q000235:genetics / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D020533:Angiogenesis Inhibitors; D043169:Endostatins; D011994:Recombinant Proteins