title: Halocafeteria seosinensis gen. et sp. nov. (Bicosoecida), a halophilic bacterivorous nanoflagellate isolated from a solar saltern.
abstract: Recently, heterotrophic nanoflagellates (HNF) have been reported to actively ingest prokaryotes in high salinity waters. We report the isolation and culture of an HNF from a Korean saltern pond of 300 per thousand salinity. The organism is biflagellated with an acronematic anterior flagellum and never glides on surfaces. The mitochondria have tubular cristae. Neither transitional helix nor spiral fiber were observed in the transition zones of the flagella. The cell has a cytostome supported by an arc of eight microtubules, suggesting that our isolate is a bicosoecid. Our isolate had neither mastigonemes, lorica, body scales, nor cytopharynx and thus could not be placed in any of the presently described bicosoecid genera. Phylogenetic analysis of 18S rRNA gene sequences from stramenopiles confirmed the bicosoecid affinities of our isolate, but did not place it within any established genus or family. Its closest relatives include Caecitellus and Cafeteria. The optimal range of growth temperature was 30-35 degrees C. The isolated HNF grew optimally at 150 per thousand salinity and tolerated up to 363 per thousand salinity, but it failed to grow below 75 per thousand salinity, indicating that it could be a borderline extreme halophile. On the basis of its morphological features and position in 18S rRNA trees we propose a novel genus for our isolate; Halocafeteria, n. gen. The species name Halocafeteria seosinensis sp. nov. is proposed.
authors.affiliation: Molecular and Microbial Ecology Laboratory, School of Earth and Environmental Sciences, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Jong S
authors.initials: JS
authors.lastname: Park
authors.forename: Byung C
authors.initials: BC
authors.lastname: Cho
authors.forename: Alastair G B
authors.initials: AG
authors.lastname: Simpson
mesh_terms: D000818:Animals; D016054:DNA, Protozoan; D056890:Eukaryota / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D005407:Flagella / Q000648:ultrastructure; D007723:Korea; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D021521:Ribotyping; D012623:Seawater / Q000737:chemistry / Q000469:parasitology*; D012965:Sodium Chloride / Q000032:analysis / Q000378:metabolism*; D013696:Temperature
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S; D012965:Sodium Chloride