Clustering assay for studying the interaction of membrane proteins with PDZ domain proteins.
Some membrane proteins must be clustered at target sites to efficiently perform their functions. PDZ domain-containing scaffold proteins bind to the tails of target membrane proteins and promote their localization and clustering on the cell surface. This chapter describes the experimental details of the clustering assay, using the interaction between potassium channels and PSD-95, an abundant PDZ domain protein in neuronal synapses, as a model.
Ko
Jaewon
J
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon.
Kim
Eunjoon
E
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D019556:COS Cells; D002522:Chlorocebus aethiops; D000076122:Disks Large Homolog 4 Protein; D051528:Guanylate Kinases; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051665:Kv1.4 Potassium Channel / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D017434:Protein Structure, Tertiary
D000076122:Disks Large Homolog 4 Protein; C501447:Dlg4 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D051665:Kv1.4 Potassium Channel; D008565:Membrane Proteins; D051528:Guanylate Kinases