title: Oxidative stress and post-transplant hypertension in pediatric kidney-transplanted patients.
abstract: OBJECTIVE
  To test the hypothesis that oxidative stress signaling contributes to post-transplant endothelial dysfunction and hypertension in pediatric post-transplant hypertension.
  
  
  METHODS
  This study evaluated in 16 pediatric renal transplant patients, divided in two groups based on the presence of post-transplant hypertension, the oxidative stress status measuring the gene expression (reverse transcription-polymerase chain reaction) of two major oxidative stress-related proteins, p22(phox) and heme oxygenase-1 (HO-1). Total plasma antioxidant power (ELISA) was also evaluated.
  
  
  RESULTS
  Mononuclear cell p22(phox) gene expression was higher in hypertensive patients compared with the normotensive group (0.91 +/- 0.06 vs 0.79 +/- 0.08 densitometric units, P < .02), whereas HO-1 RNA production and total plasma antioxidant power were higher in the normotensive group (0.38 +/- 0.04 vs 0.20 +/- 0.11 d.u., P < .006, and 1189.35 +/- 145.75 vs 772.71 +/- 196.03 micromol/L, P < .01, respectively).
  
  
  CONCLUSIONS
  Oxidative stress is associated with post-transplant hypertension in hypertensive pediatric kidney-transplant patients, who therefore are at risk of oxidative stress-induced organ damage. They might benefit from treatments addressing not only hypertension but also oxidant-related complications.
authors.affiliation: Clinical and Experimental Medicine, Clinica Medica 4, and Pediatric Nephrology University of Padova, Italy. renzcalo@unipd.it
authors.forename: Lorenzo A
authors.initials: LA
authors.lastname: Calò
authors.forename: Roberto
authors.initials: R
authors.lastname: Dall'Amico
authors.forename: Elisa
authors.initials: E
authors.lastname: Pagnin
authors.forename: Lara
authors.initials: L
authors.lastname: Bertipaglia
authors.forename: Graziella
authors.initials: G
authors.lastname: Zacchello
authors.forename: Paul A
authors.initials: PA
authors.lastname: Davis
mesh_terms: D000293:Adolescent; D000975:Antioxidants / Q000032:analysis; D003300:Copper / Q000097:blood; D005260:Female; D015870:Gene Expression; D051547:Heme Oxygenase-1 / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D019255:NADPH Oxidases / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D003300:Copper; D051547:Heme Oxygenase-1; D019255:NADPH Oxidases; C086986:CYBA protein, human