High-performance liquid chromatographic determination of doxorubicin in tissues after solid phase extraction.
A method is described for the determination of doxorubicin in tissues. The drug was selectively extracted from the biological matrix by solid phase extraction using 1-ml octadecyl silane extraction columns. Prior to extraction, tissue samples were digested by an enzymic digestion procedure. Daunorubicin was used as an internal standard. Quantitation was by high-performance liquid chromatography (HPLC) using ion-pair chromatography on a reversed-phase column. Detection was by fluorescence. Recovery of doxorubicin from tissue was 81.7 +/- 2.1% mean +/-SD. Doxorubicin concentrations as low as 0.01 mg kg(-1) could be determined. A typical value of the relative standard error of measurement was 3.7% at 2.1 mg kg(-1).
de Groot
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Laboratory for Residue Analysis, National Institute of Public Health and Environmental Hygiene, P.O. Box 1, 3720 BA Bilthoven, The Netherlands.
Tepas
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Storm
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