Decreased follicular size during late lactation caused by treatment with charcoal-treated follicular fluid delays onset of estrus and ovulation after weaning in sows.
The weaning to estrus and weaning to ovulation intervals in sows are controlled by ovarian follicular growth after weaning. Longer intervals could be caused by smaller diameter follicles at weaning that take more time to reach a preovulatory size. We addressed this hypothesis by decreasing the diameter of follicular populations before weaning and then measuring follicular development and interval to estrus and ovulation after weaning. The posterior vena cava, cranial to the entry of the ovarian vein, was cathetered for blood sampling and infusion in 20 sows at 12 +/- 1 d after farrowing. Sows were assigned randomly to receive either 30 mL of charcoal-treated follicular fluid (FF, n = 9; a treatment known to decrease serum FSH and follicular diameter) or 30 mL of saline (n = 11) by venous infusion thrice daily (0700, 1500, and 2300 h) for 96 h beginning at 14 +/- 1 d after farrowing. Sows were weaned 48 h after the last infusion. Blood samples were collected for FSH analysis thrice daily beginning on the day of catheterization and continuing until ovulation. Follicular diameter was determined once daily by transrectal ultrasonography. A treatment x time interaction was detected for serum FSH (P < 0.001) and follicular diameter (P < 0.001) because serum FSH and the diameter of follicular populations decreased in FF sows during the infusion period. After the infusion period, serum FSH rebounded in FF sows, and follicles resumed growth but grew at the same rate as those of saline-treated sows, thus failing to achieve equivalent diameters relative to saline-treated sows on a given day after weaning. As a result, sows treated with FF had longer (P < 0.05) weaning to estrus (6.1 +/- 0.4 d) and weaning to ovulation (8.6 +/- 0.5 d) intervals compared with saline-treated sows (4.7 +/- 0.4 d and 7.2 +/- 0.4 d, respectively). We conclude that the diameter of the follicular population at weaning is one factor that controls interval to estrus and ovulation in sows. Small follicles at weaning cannot undergo compensatory growth and require additional time to reach a preovulatory size.
Bracken
C J
CJ
Department of Animal Sciences, University of Missouri, Columbia 65211, USA.
Radcliff
R P
RP
McCormack
B L
BL
Keisler
D H
DH
Lucy
M C
MC
D000818:Animals; D002606:Charcoal / Q000737:chemistry* / Q000494:pharmacology; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D015571:Follicular Fluid / Q000737:chemistry*; D007774:Lactation / Q000502:physiology*; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D010060:Ovulation / Q000187:drug effects* / Q000502:physiology; D013552:Swine / Q000502:physiology*; D014886:Weaning
D002606:Charcoal; D004958:Estradiol; D005640:Follicle Stimulating Hormone