The biofilm matrix of Pseudomonas sp. OX1 grown on phenol is mainly constituted by alginate oligosaccharides.
The structure of the major constituent of the biofilm matrix produced by Pseudomonas sp. OX1, when grown on phenol as the sole carbon source is described. This investigation, carried out by chemical analysis, NMR spectroscopy and MALDI-TOF MS spectrometry, showed the presence of an oligosaccharide blend with the typical alginate structure, namely (1-->4) substituted beta-D-mannuronic (ManA) and alpha-L-guluronic acid (GulA). GulA residues were non-acetylated whereas ManA was always O-acetylated at C-2 or C-3.
Leone
Serena
S
Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Via Cintia, 4 I-80126 Napoli, Italy.
Molinaro
Antonio
A
Alfieri
Fabiana
F
Cafaro
Valeria
V
Lanzetta
Rosa
R
Di Donato
Alberto
A
Parrilli
Michelangelo
M
D000464:Alginates / Q000737:chemistry*; D018441:Biofilms; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry*; D019800:Phenol; D011135:Polysaccharides, Bacterial; D011549:Pseudomonas / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000464:Alginates; D006603:Hexuronic Acids; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D019800:Phenol; D020723:Glucuronic Acid