title: Determination of ibuprofen in human plasma by solid phase extraction and reversed-phase high-performance liquid chromatography.
abstract: A new, sensitive and simple method for the rapid quantitative determination of ibuprofen in human plasma has been developed. This method involves the use of a solid phase extraction on "Baker" C-18 disposable extraction columns for sample clean-up and uses mefenamic acid as an internal standard. Separation and quantitation are performed by reversed-phase liquid chromatography using a Nucleosil C18 column and methanol-0.04 M phosphoric acid (80:20, v/v), as the mobile phase. Detection was achieved by UV-absorbance measurements at 229 nm.
authors.affiliation: Pharma Bio-Research International B. V., Department of Biopharmaceutics and Clinical Pharmacokinetics, P.O. Box 147, 9400 AC Assen, The Netherlands.
authors.forename: J H
authors.initials: JH
authors.lastname: Jonkman
authors.forename: R
authors.initials: R
authors.lastname: Schoenmaker
authors.forename: A H
authors.initials: AH
authors.lastname: Holtkamp
authors.forename: J
authors.initials: J
authors.lastname: Hempenius