[Investigation of biodegradable materials as polymeric gene carriers].
This is a study on the biodegradable polymers as gene controlled-released coatings for gene transfer. The PELA (poly (Dl-lactic acid)-co-poly (ethylene glycol), and PLGAE (poly (lactic acid)-co-poly (ethylene glycol)-co-poly (glycolic acid) random copolymer) were synthesized and prepared as the coatings of plasmid pCH110 in the transfection. All kinds of factors affecting the loading efficiency, cytotoxicity, transfection efficiency and the course of the degradation and release in vitro were discussed. The average diameters of microspheres of PELA and PLGAE were 1-3 microm and 0.72 microm respectively. The loading efficiency levels of them were 62% and 70% respectively. The transfection efficiency levels of two kinds of pCH110 delivery system for COS-1 cells were higher and two of them had few cytotoxicity. After transfection, the X-gal assay was performed and reported positive for 96 h. The biodegradable polymeric materials as gene carriers possess their potential superiority.
Yang
Yuan
Y
Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 6 0041, China.
Jia
Wenxiang
W
Qi
Xin
X
Zeng
Wei
W
Yang
Falong
F
Xie
Yi
Y
Yang
Weiqing
W
Zhang
Zairong
Z
Zhou
Shaobing
S
Li
Xiaohong
X
D001672:Biocompatible Materials; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D018014:Gene Transfer Techniques; D007773:Lactates / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D011100:Polyglycolic Acid / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
D001672:Biocompatible Materials; D004337:Drug Carriers; D007773:Lactates; D011108:Polymers; C058523:polyethylene oxide-polylactic acid block copolymer; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; D004247:DNA