[Heterogeneity of the populations of marine luminescent bacteria Photobacterium leiognathi under different conditions of cultivation].
Manifestation of pleiotropic effects in the isogenic variants of luminescent bacteria Photobacterium leiognathi 54 was investigated. The decrease or increase of the expression level of bioluminescence was caused by changes in lux operon regulation. The dynamics of the bioluminescence of dark and dim variants did not differ from the dynamics of the initial luminescent variant, but dependence of the level of luminescence intensity on the exogenous autoinductor of the lux operon was revealed. The investigated variants of P. leiognathi 54 inherited fairly stable morphological characteristics, colony architectonics, level of luminescence, and activity of some enzymes; variants with reduced bioluminescence formed colonies of the S type. Stable bright variants with S- and R-type colonies appeared both in the initial strain population and in the dark variant population, but with smaller frequency. Populations of the bright variant with R-type colonies were most heterogeneous; this can be determined by the lack of glucose repression of the bioluminescence in contrast to other investigated variants of P. leiognathi.
Medvedeva
S E
SE
Mogil'naia
O A
OA
Popova
L Iu
LIu
D008156:Luciferases / Q000032:analysis; D008163:Luminescent Measurements; D008854:Microscopy, Electron; D009792:Oceans and Seas; D010641:Phenotype; D010776:Photobacterium / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D013045:Species Specificity; D013997:Time Factors; D014871:Water Microbiology
D008156:Luciferases