[Preliminary application on test paper of gold-labelled antibody against fibronectin EIIIA splicing variant].
OBJECTIVE
To develop the test Paper of gold-labelled antibody against fibronectin EIIIR A splicing variant, which can be available in forensic wound interval estimation.


METHODS
Two sensitive antibodies were compared with enzyme link immunoabsorband assay (ELISA). After colloid gold labeled, the effects of the two antibodies were tested by methods of Dot immunogold filtration assay and gold immunochromatography assay, respectively. The test paper was finally appraised by applied in experimental skin injury in rats.


RESULTS
On the test paper, detected line appeared in three hours wound age group, and then the darkness of positive staining became darker with injury time prolonging, while control normal skin cannot found to be positive staining.


CONCLUSIONS
The gold-labeled test paper can be useful in estimation of wounding interval in forensic science.
Liu
Ning-Guo
NG
Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, China. liuningguo@yahoo.com.cn
Chen
Yi-Jiu
YJ
Lu
Ren-Quan
RQ
Huang
Xiao-Hua
XH
Zhang
Jian-Hua
JH
D017398:Alternative Splicing; D000818:Animals; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000378:metabolism*; D049429:Forensic Pathology; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011934:Reagent Strips; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D013997:Time Factors; D014949:Wounds, Nonpenetrating / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D017979:Gold Colloid; D011934:Reagent Strips