title: Direct evidence for enzyme persulfide and disulfide intermediates during 4-thiouridine biosynthesis.
abstract: Two proposed mechanisms for 4-thiouridine generation share key cysteine persulfide and disulfide intermediates, and indirect evidence of their existence has been previously reported; chemical trapping and mass spectrometry have now provided direct and definitive evidence of these key intermediates.
authors.affiliation: Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
authors.forename: Chapman M
authors.initials: CM
authors.lastname: Wright
authors.forename: Glenn D
authors.initials: GD
authors.lastname: Christman
authors.forename: Ann M
authors.initials: AM
authors.lastname: Snellinger
authors.forename: Murray V
authors.initials: MV
authors.lastname: Johnston
authors.forename: Eugene G
authors.initials: EG
authors.lastname: Mueller
mesh_terms: D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D015394:Molecular Structure; D013891:Thiouridine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004220:Disulfides; D004798:Enzymes; D013891:Thiouridine; C116840:cysteine persulfide; D003545:Cysteine