Mutations and promoter methylation status of NPM1 in myeloproliferative disorders.
We determined mutations and promoter methylation status of NPM1 using pyrosequencing in 199 samples of myeloid neoplasia including myeloproliferative disorders (MPD). The mutations were present in 4% of chronic myelomonocytic leukemia, but not in other MPD or myelodysplastic syndromes. Promoter methylation was rare, and was found in only three samples of MPD.
Oki
Yasuhiro
Y
Jelinek
Jaroslav
J
Beran
Miloslav
M
Verstovsek
Srdan
S
Kantarjian
Hagop M
HM
Issa
Jean-Pierre J
JP
D001483:Base Sequence; D019175:DNA Methylation; D017931:DNA Primers; D014644:Genetic Variation; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics; D009154:Mutation; D009196:Myeloproliferative Disorders / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D000090243:Nucleophosmin; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic
D017931:DNA Primers; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D000090243:Nucleophosmin