[Conservation of Malassezia strains in blotting paper].
Reference strains belonging to the genus Malassezia were analyzed to evaluate, by comparison, different preservation systems such us subculture, freezing at -80 degrees C in glycerol, and blotting paper-disc conservation. The viability, phenotypic and genotypic characteristics of the strains used in this study was evaluated. The blotting paper method was found to be advantageous to preserve Malassezia spp strains due to both, its simple implementation in the laboratory and its efficiency.
Ramos
Laura
L
Centro de Referencia en Micología, Facultad de Ciencias Bioquímicas y Farmaciéuticas, Suipacaha 531, 2000 Universidad Nacional de Rosario, Rosario, Argentina. ramos.laura@tower.com.ar
Ramadán
Silvana
S
López
Clara
C
Bulacio
Lucía
L
Mellado
Soledad
S
D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D005838:Genotype; D005990:Glycerol; D008290:Malassezia / Q000187:drug effects / Q000235:genetics; D009172:Mycology / Q000379:methods* / Q000592:standards; D010209:Paper; D010641:Phenotype; D011309:Preservation, Biological; D012015:Reference Standards
D003451:Cryoprotective Agents; D005990:Glycerol