Association of Gln222Arg polymorphism in the deoxyribonuclease I (DNase I) gene with myocardial infarction in Japanese patients.
OBJECTIVE
We have recently reported that serum deoxyribonuclease I (DNase I) activity, which may be involved in apoptosis, increases abruptly in the early phase of acute myocardial infarction (MI) [Kawai Y, Yoshida M, Arakawa K, Kumamoto T, Morikawa N, Masamura K, Tada H, Ito S, Hoshizaki H, Oshima S, Taniguchi K, Terasawa H, Miyamori I, Kishi K, Yasuda T. Diagnostic use of serum deoxyribonuclease I activity as a novel early-phase marker in acute myocardial infarction. Circulation 2004;109:2398-2400]. Death of vascular smooth muscle cells, in part because of apoptosis, is postulated to heighten susceptibility to disruption of vulnerable plaque, resulting in onset of MI. The present study evaluated the possibility that Gln222Arg polymorphism of the DNase I gene may be one of the factors involved in predisposition to MI.


RESULTS
We assessed 611 Japanese patients: 311 with MI and 300 with stable angina pectoris (AP). Three common phenotypes determined by two common codominant alleles, DNASE1*1 and *2, whose corresponding gene products exhibit different properties, were found in these patient groups. The prevalence of DNASE1*2 was significantly higher in patients with MI than in those with AP (0.543 vs. 0.428, P < 0.001), being confirmed by phenotyping of the second study population. Multiple logistic regression analysis showed that the odds ratio of DNASE1*2 was 1.51 [95% confidence interval (CI) 1.04-2.18]. The association of the DNASE1*2 allele with MI was statistically significant, being independent of other conventional risk factors.


CONCLUSIONS
Our data demonstrate that Gln222Arg polymorphism in the DNase I gene is associated with MI in the Japanese patients.
Kumamoto
Teruhiko
T
Third Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.
Kawai
Yasuyuki
Y
Arakawa
Kenichiro
K
Morikawa
Norihiro
N
Kuribara
Jun
J
Tada
Hiroshi
H
Taniguchi
Koichi
K
Tatami
Ryozo
R
Miyamori
Isamu
I
Kominato
Yoshihiko
Y
Kishi
Koichiro
K
Yasuda
Toshihiro
T
D000368:Aged; D000787:Angina Pectoris / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D003850:Deoxyribonuclease I / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D012044:Regression Analysis; D012307:Risk Factors
D003850:Deoxyribonuclease I