Proteomic analysis of human neutrophils.
Proteomics is the study of the set of proteins, or proteome, expressed by a cell under specific conditions. Proteomics methodology consists of protein extraction, protein separation, and protein identification. Currently, two-dimensional gel electrophoresis (2DE) and matrix-assisted laser-desorption ionization time of flight mass spectrometry are the most widespread methods for proteomic studies. The recent introduction of precast immobilized pH gradient gel strips, precast gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels, and well-designed electrophoresis equipment has made 2DE a highly reproducible and relatively simple method for protein separation. Inherent limitations of the procedure, however, require approaches in sample preparation that may be cell- or tissue-dependent. This chapter describes a methodology for proteomic analysis of human neutrophils and discusses its applications.
Lominadze
George
G
Biochemistry and Molecular Biology, University of Louisville, KY, USA.
Ward
Richard A
RA
Klein
Jon B
JB
McLeish
Kenneth R
KR
D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods; D008970:Molecular Weight; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*; D010455:Peptides / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D015398:Signal Transduction / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D010455:Peptides; D020543:Proteome