The antigenic determinants on HIV p24 for CD4+ T cell inhibiting antibodies as determined by limited proteolysis, chemical modification, and mass spectrometry.
In the last decade, mass spectrometry has been employed by more and more researchers for identifying the proteins in a macromolecular complex as well as for defining the surfaces of their binding interfaces. This characterization of protein-protein interfaces usually involves at least one of several different methodologies in addition to the actual mass spectrometry. For example, limited proteolysis is often used as a first step in defining regions of a protein that are protected from proteolysis when the protein of interest is part of a macromolecular complex. Other techniques used in conjunction with mass spectrometry for determining regions of a protein involved in protein-protein interactions include chemical modification, such as covalent cross-linking, acetylation of lysines, hydrogen-deuterium exchange, or other forms of modification. In this report, both limited proteolysis and chemical modification were combined with several mass spectrometric techniques in efforts to define the protein surface on the HIV core protein, p24, recognized by two different monoclonal human antibodies that were isolated from HIV+ patients. One of these antibodies, 1571, strongly inhibits the CD4+ T cell proliferative response to a known epitope (PEVIPMFSALSEGATP), while the other antibody, 241-D, does not inhibit as strongly. The epitopes for both of these antibodies were determined to be discontinuous and localized to the N-terminus of p24. Interestingly, the epitope recognized by the strongly inhibiting antibody, 1571, completely overlaps the T cell epitope PEVIPMFSALSEGATP, while the antibody 241-D binds to a region adjacent to the region of p24 recognized by the antibody 1571. These results suggest that, possibly due to epitope competition, antibodies produced during HIV infection can negatively affect CD4+ T cell-mediated immunity against the virus.
Williams
Jason G
JG
National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Laboratory of Structural Biology, 111 TW Alexander Drive, MD F0-03, 27709, Research Triangle Park, NC, USA.
Tomer
Kenneth B
KB
National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Laboratory of Structural Biology, 111 TW Alexander Drive, MD F0-03, 27709, Research Triangle Park, NC, USA. tomer@niehs.nih.gov.
Hioe
Catarina E
CE
New York Veterans Affairs Medical Center and Department of Pathology, New York University School of Medicine, New York, New York, USA.
Zolla-Pazner
Susan
S
New York Veterans Affairs Medical Center and Department of Pathology, New York University School of Medicine, New York, New York, USA.
Norris
Philip J
PJ
Blood Systems Research Institute and Departments of Laboratory Medicine and Medicine, University of California-San Francisco, San Francisco, California, USA.
D000595:Amino Acid Sequence; D019138:Antibodies, Blocking / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D000939:Epitopes; D016655:HIV Core Protein p24 / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D011487:Protein Conformation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D000939:Epitopes; D016655:HIV Core Protein p24