title: Genotypes of Listeria monocytogenes strains isolated from 2000 to 2002 in Poland.
abstract: Pulsed field gel electrophoresis (PFGE), multiplex PCR and multilocus sequence typing (MLST) methods were used for genotyping study of seventy-three L. monocytogenes isolates collected in Poland between 2000 and 2002 from human, food, environment and a diseased goat. The multiplex PCR, which is an alternative method to classical serotyping, divided the isolates into four PCR groups, IIa (42.5%), IIb (27.4%), IIc (4.1%) and IVb (26%). The isolates displayed 33 distinct PFGE profiles. Twenty eight strains were further characterised by MLST based on sequence analyses of seven housekeeping genes. The combined sequence analyses revealed a total of 10 different allelic profiles from which 3 were not described earlier. It is intended that results obtained in this study will be the first data of a national database of L. monocytogenes genotypes in Poland.
authors.affiliation: Voivodship Sanitary--Epidemiological Station in Warsaw, 79 Zelazna St., 00-875 Warsaw, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Paciorek
authors.forename: Christine
authors.initials: C
authors.lastname: Jacquet
authors.forename: Celia
authors.initials: C
authors.lastname: Salcedo
authors.forename: Michel
authors.initials: M
authors.lastname: Doumith
authors.forename: Julio A
authors.initials: JA
authors.lastname: Vázquez
authors.forename: Paul
authors.initials: P
authors.lastname: Martin
mesh_terms: D000818:Animals; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005838:Genotype; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics*; D010641:Phenotype; D011044:Poland