title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates.
abstract: Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same pattern. These findings are not consistent with a prior report. Differences in 23S rRNA gene sequences, between two B. murdochii strains, 56-150 and 155-20, were observed. These results indicate that 23S rRNA PCR-RFLP could be used as an identification method for pathogenic Brachyspira spp. (B. hyodysenteriae and B. pilosicoli) as well as an epidemiological tool for characterizing spirochetes isolated from swine.
authors.affiliation: School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Kim
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Lee
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D004405:Dysentery, Bacillary / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D007723:Korea; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics*; D013144:Spirochaetales / Q000235:genetics* / Q000302:isolation & purification*; D013145:Spirochaetales Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S