title: Mixed monolayer-protected gold nanoclusters as selective peptide extraction agents for MALDI-MS analysis.
abstract: Cationic and anionic nanoparticles selectively target peptides with low and high isoelectric points, respectively. Additionally, their high surface area-to-volume ratios make these nanoparticles (approximately 2-nm core diameter) very efficient extraction and concentration agents. Upon extraction, the peptide-bound nanoparticles can be analyzed by MALDI-MS to provide highly sensitive detection of the targeted peptides. We demonstrate that MALDI-MS can detect peptide concentrations as low as 500 pM from 250-microL solutions using these nanoparticle scaffolds as extraction and concentration agents.
authors.affiliation: Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors.forename: Benjamin N Y
authors.initials: BN
authors.lastname: Vanderpuije
authors.forename: Gang
authors.initials: G
authors.lastname: Han
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
authors.forename: Richard W
authors.initials: RW
authors.lastname: Vachet
mesh_terms: D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D010455:Peptides; D006046:Gold