Recombinant-protein production in insect cells utilizing a hollow-fibre bioreactor.
Here, we demonstrate for the first time that the hollow-fibre bioreactor is an excellent tool for the production of Drosophila-expressed recombinant proteins. Using the example of the soluble extracellular portion of the human IL-5 (interleukin 5) receptor alpha expression in S2 (Schneider's Drosophila melanogaster cell line 2) cells, we found that it is possible to produce multi-milligram amounts of functional recombinant protein continuously for several months on a laboratory scale with minimal maintenance requirements. The insect cells grow to high density and express concentrated functional recombinant protein in a small volume, simplifying and economizing downstream purification.
Baxter
Sabine C
SC
Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 11102 New College Building, 245 N. 15th Street, Philadelphia, PA 19102, USA. scb28@drexel.edu
Panarello
Dominick
D
Ajith
Sandya
S
Bhattacharya
Madhushree
M
Chaiken
Irwin M
IM
D000818:Animals; D001667:Binding, Competitive; D019149:Bioreactors; D015374:Biosensing Techniques; D002478:Cells, Cultured; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D053666:Interleukin-5 Receptor alpha Subunit / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020349:Surface Plasmon Resonance
C485918:IL5RA protein, human; D053666:Interleukin-5 Receptor alpha Subunit; D011994:Recombinant Proteins