[Screening of short peptides binding to cell surface interleukin-2 receptor alpha chain].
OBJECTIVE
To screen and characterize the short peptides which bind specifically to interleukin-2 (IL-2) receptor alpha chain (IL-2Ralpha) for acquisition of small antagonists for blocking the binding of IL-2 with IL-2Ralpha.


METHODS
12-mer phage displayed peptide library was screened with the target cells of MT-2 cells which expressed IL-2Ralpha at high levels. The binding phage clones were eluted by anti-IL-2Ralpha monoclonal antibody. After 3 rounds of screening, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, and the amino acid sequences of the positive clones were deduced from the DNA sequences.


RESULTS
Seven positive clones were screened out of the 17 phage clones bound to MT-2 cells. The positive clone M15 could bind specifically to MT-2 cell and PHA-activated peripheral blood monouclear cells. Amino acid sequence analysis identified 6 sequences, all of which contained hydrophilic residues, and 5 of these 6 sequences included Tyr, Phe and Leu conservative residues.


CONCLUSIONS
The peptide sequences containing Tyr, Phe conservative residues identified in this study can bind to cell surface IL-2Ralpha.
Liu
Bei-yi
BY
Department of Immunology, Southern Medical University, Guangzhou 510515, China. beiyi@fimmu.com
Zhu
Ping
P
Luo
Hai-bo
HB
Fu
Ning
N
D000595:Amino Acid Sequence; D002461:Cell Line, Transformed; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D007376:Interleukin-2 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism
D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D019151:Peptide Library; D010455:Peptides; D011956:Receptors, Cell Surface