[Study on colloidal gold labeled anti-buprenorphine monoclonal antibody for rapid test kit to detect buprenorphine].
OBJECTIVE
To develope an easy to use, rapid and accurate test for detecting buprenorphine based on the principle of competitive immunoassay.


METHODS
Monoclonal antibody against buprenorphine was conjugated with colloidal gold and dispensed on the glass fiber. The complete antigen Buprenorphine-BSA and the goat anti-mouse IgG polyclonal antibody were separately sprayed on the nitrocellulose membrane as the test line (T line) and the control line (C line). The rapid test kit was the final assembled product of test strip with the plastic cover.


RESULTS
A total of 100 urine samples were tested for buprenorphine by immunochromatographic and GC/ MS methods. The accuracy was 99.0%. It is found the test kit can only detect by cross reaction with other 45 kind drugs.


CONCLUSIONS
Rapid test kit can detect buprenorphine in the samples in 5 minutes. The cut-off value of the test is 100 ng/mL.
Zeng
Li-Bo
LB
Shanghai Forensic Science and Technology Research Institute, Shanghai 200083, China.
Chen
Lian-Kang
LK
Hu
Xiao-Long
XL
Chen
Liang
L
Wang
Xue-Sheng
XS
Zhang
Yu-Rong
YR
D000701:Analgesics, Opioid / Q000032:analysis / Q000652:urine; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D002047:Buprenorphine / Q000032:analysis* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D017979:Gold Colloid; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000701:Analgesics, Opioid; D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D011933:Reagent Kits, Diagnostic; D002047:Buprenorphine