title: Yeast homolog of a cancer-testis antigen defines a new transcription complex.
abstract: We have isolated a new yeast gene (PCC1) that codes for a factor homologous to human cancer-testis antigens. We provide evidence that Pcc1p is a new transcription factor and that its mutation affects expression of several genes, some of which are involved in cell cycle progression and polarized growth. Mutation of Pcc1p also affects the expression of GAL genes by impairing the recruitment of the SAGA and Mediator co-activators. We characterize a new complex that contains Pcc1p, a kinase, Bud32p, a putative endopeptidase, Kae1p and two additional proteins encoded by ORFs YJL184w and YMLO36w. Genetic and physical interactions among these proteins strongly suggest that this complex is a functional unit. Chromatin immunoprecipitation experiments and multiple genetic interactions of pcc1 mutants with mutants of the transcription apparatus and chromatin modifying enzymes underscore the direct role of the complex in transcription. Functional complementation experiments indicate that the transcriptional function of this set of genes is conserved throughout evolution.
authors.affiliation: Centre National de la Recherche Scientifique, Centre de Genetique Moleculaire, Gif sur Yvette, Paris, France.
authors.forename: Elena
authors.initials: E
authors.lastname: Kisseleva-Romanova
authors.forename: Raffaele
authors.initials: R
authors.lastname: Lopreiato
authors.forename: Agnès
authors.initials: A
authors.lastname: Baudin-Baillieu
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Rousselle
authors.forename: Laila
authors.initials: L
authors.lastname: Ilan
authors.forename: Kay
authors.initials: K
authors.lastname: Hofmann
authors.forename: Abdelkader
authors.initials: A
authors.lastname: Namane
authors.forename: Carl
authors.initials: C
authors.lastname: Mann
authors.forename: Domenico
authors.initials: D
authors.lastname: Libri
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D021122:Protein Subunits / Q000235:genetics; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D017385:Sequence Homology; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology*; D014158:Transcription, Genetic; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D002843:Chromatin; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; C513653:PCC1 protein, S cerevisiae; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C513654:Yjl184w protein, S cerevisiae; C513655:Yml036w protein, S cerevisiae; C108301:BUD32 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; D012319:RNA Polymerase II; C480738:Kae1 protein, S cerevisiae; D008666:Metalloendopeptidases