title: [Screening of peptide inhibitors of acetylcholinesterase from 12-mer random phage display peptide library].
abstract: OBJECTIVE
  To screen the peptide inhibitor of acetylcholinesterase (AChE) from 12-mer random phage display peptide library.
  
  
  METHODS
  Human AChE was used as the target to screen its binding peptides from 12-mer random phage display peptide library. The positive phage clones were isolated after three rounds of biopanning followed then by sequence analysis and their activity evaluation.
  
  
  RESULTS
  Six positive phage clones binding to human AChE were obtained, and 4 of them sharing the conservative sequence W(S/P)HY inhibited the enzyme activity of AChE.
  
  
  CONCLUSIONS
  Acquisition of AChE inhibitor from phage display library provides clues for designing peptide inhibitors of AChE.
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China. shiys@fimmu.com
authors.forename: Xing-mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Yu-sheng
authors.initials: YS
authors.lastname: Shi
authors.forename: Chun-xia
authors.initials: CX
authors.lastname: Wang
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding
chemical_list: D002800:Cholinesterase Inhibitors; D019151:Peptide Library; D010455:Peptides; D000110:Acetylcholinesterase