[A minisequencing technique for genotyping 12 Y-SNP and its genetic polymorphisms].
OBJECTIVE
The aim of the present study was to establish a rapid and robust assay used to simultaneously genotype SNPs by the single nucleotide primer extension (minisequencing) with the SNaPshot Kit and obtain the population genetic data in Chinese population in Sichuan. The analysis of single nucleotide polymorphisms (SNPs) is a promising application in forensic casework.


METHODS
12 Y-SNPs, which were SRY2627, SRY1532, M13, M20, SRY8299, Tat, M69, M9, 92R7, M17, M19 and M112, were multiple amplificated and the PCR products were pooled, Purified, and then used as templates for the minisequencing reaction with the commol/Lercially available SNaPshot Kit. Then the products of minisequencing reaction were detected by capillary elcetrophoresis.


RESULTS
78 genomic DNA individual samples from Sichuan, China and 5 semen stain samples from sexal criminal scene were analyzed and two haplotypes could be identified.


CONCLUSIONS
A rapid method has been established to analyze the 12 Y-SNPs by multiplex PCR and minisequencing. It can be applied in forensic casework successfully.
Du
Hong
H
Department of Criminal technique, Sichuan Public Security Bureau, Chengdu 610041, China.
Zhang
Lin
L
Zhou
Bin
B
Zhang
Hai-Jun
HJ
Liang
Wei-Bo
WB
Shen
Yue-Hua
YH
D001483:Base Sequence; D001842:Bone and Bones / Q000737:chemistry; D002681:China / Q000208:ethnology; D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D019075:Electrophoresis, Capillary; D005554:Forensic Medicine; D005787:Gene Frequency; D005819:Genetic Markers; D005828:Genetics, Population; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*; D012742:Sex Offenses; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA