title: Microtubule cytoskeleton behavior in the initial steps of host cell invasion by Besnoitia besnoiti.
abstract: Besnoitia besnoiti is a protozoan parasite responsible for bovine besnoitiosis. Indirect immunofluorescence showed that isolated B. besnoiti possesses a set of subpellicular microtubules, radiating from the apical end and extending for more than 2/3 of the cell body. Upon interaction with the host cell, B. besnoiti undergoes dramatic modifications of shape and surface, as revealed by atomic force microscopy, accompanied by a distinct tubulin labeling on the posterior region. In the host cell, the microtubule cytoskeleton shows a re-arrangement around the invading parasite suggesting a filamentous interaction with the parasite cytoskeleton during invasion.
authors.affiliation: Instituto de Investigação Científica Tropical, CVZ, CIISA, Av. Universidade Técnica, Lisboa 1300-477, Portugal.
authors.forename: Y
authors.initials: Y
authors.lastname: Reis
authors.forename: H
authors.initials: H
authors.lastname: Cortes
authors.forename: L
authors.initials: L
authors.lastname: Viseu Melo
authors.forename: I
authors.initials: I
authors.lastname: Fazendeiro
authors.forename: A
authors.initials: A
authors.lastname: Leitão
authors.forename: H
authors.initials: H
authors.lastname: Soares
mesh_terms: D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D008870:Microtubules / Q000502:physiology* / Q000648:ultrastructure; D021745:Sarcocystidae / Q000502:physiology* / Q000648:ultrastructure