Quantitative assessment of olfactory receptors activity in immobilized nanosomes: a novel concept for bioelectronic nose.
We describe how mammalian olfactory receptors (ORs) could be used as sensing elements of highly specific and sensitive bioelectronic noses. An OR and an appropriate G(alpha) protein were co-expressed in Saccharomyces cerevisiae cells from which membrane nanosomes were prepared, and immobilized on a sensor chip. By Surface Plasmon Resonance, we were able to quantitatively evaluate OR stimulation by an odorant, and G protein activation. We demonstrate that ORs in nanosomes discriminate between odorant ligands and unrelated odorants, as in whole cells. This assay also provides the possibility for quantitative assessment of the coupling efficiency of the OR with different G(alpha) subunits, without the interference of the cellular transduction pathway. Our findings will be useful to develop a new generation of electronic noses for detection and discrimination of volatile compounds, particularly amenable to micro- and nano-sensor formats.
Vidic
Jasmina Minic
JM
Unité de Neurobiologie de l'Olfaction et de la Prise Alimentaire, Equipe Récepteurs et Communication Chimique, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cedex, France. jasmina.minic@jouy.inra.fr
Grosclaude
Jeanne
J
Persuy
Marie-Annick
MA
Aioun
Josiane
J
Salesse
Roland
R
Pajot-Augy
Edith
E
D000818:Animals; D021522:Bioartificial Organs; D015374:Biosensing Techniques; D018914:Cells, Immobilized; D044385:GTP-Binding Protein alpha Subunits / Q000737:chemistry / Q000235:genetics; D006801:Humans; D051379:Mice; D053758:Nanoparticles; D009812:Odorants / Q000032:analysis*; D051381:Rats; D018035:Receptors, Odorant / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
D044385:GTP-Binding Protein alpha Subunits; D018035:Receptors, Odorant; D011994:Recombinant Proteins