title: Diffusion of single star-branched dendrimer-like DNA.
abstract: We report a single-molecule fluorescence study on the diffusion of star-branched polymer dendrimer-like DNA (DL-DNA). The DL-DNA molecules were synthesized from ligating Y-shaped DNA to the fourth generation. It was found through single particle tracking that the diffusion coefficient of DL-DNA changes in a nonmonotonic fashion with its increased concentration possibly due to arm arrest and arm retraction. The diffusion of DL-DNA in linear lambda DNA solution displayed a monotonic concentration dependence 1 order of magnitude greater than the diffusion of DL-DNA in DL-DNA solution. This difference is attributed to the different conformation of DL-DNA and lambda DNA and the entanglement of lambda DNA with a large radius of gyration. Our diffusion study facilitates DL-DNA transportation for drug delivery.
authors.affiliation: Department of Physics and Astronomy, Clemson University, Clemson, South Carolina 29634, USA.
authors.forename: Katherine O
authors.initials: KO
authors.lastname: Freedman
authors.forename: Janet
authors.initials: J
authors.lastname: Lee
authors.forename: Yougen
authors.initials: Y
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
authors.forename: Victoria B
authors.initials: VB
authors.lastname: Skobeleva
authors.forename: Pu Chun
authors.initials: PC
authors.lastname: Ke
mesh_terms: D001583:Benzoxazoles; D004396:Coloring Agents; D004247:DNA / Q000737:chemistry*; D003627:Data Interpretation, Statistical; D050091:Dendrimers / Q000737:chemistry*; D004058:Diffusion; D016503:Drug Delivery Systems; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation
chemical_list: D001583:Benzoxazoles; D004396:Coloring Agents; D050091:Dendrimers; C120457:POPO 3; D004247:DNA