Protein chemical identification and characterization of the human variants of far upstream element binding protein in medulloblastoma DAOY cell line.
The assembly of trans-acting proteins on sequence-specific DNA cis-elements is crucial in the regulation of eukaryotic gene expression. Far upstream element binding proteins (FAB) are proteins that regulate the expression of the c-myc oncogene by binding to the far upstream element of the c-myc gene. The present study unambiguously identified the two human variants of FAB (FAB1, FAB2) in the medulloblastoma DAOY cell line and characterized their structure for the first time by tandem mass spectrometry independent of antibody availability and specificity. The study also tentatively assigned the third variant (FAB3) at the level of mass spectrometry, although tandem mass spectrometric analysis failed to corroborate the result. These findings open up an exciting possibility for discerning the cellular roles of FAB in tumor biology.
Engidawork
Ephrem
E
Department of Pediatrics, Medical University of Vienna, A-1090, Vienna, Austria.
Afjehi-Sadat
Leila
L
Yang
Jae-Won
JW
Slavc
Irene
I
Lubec
Gert
G
D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002528:Cerebellar Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D004265:DNA Helicases / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008527:Medulloblastoma / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D016601:RNA-Binding Proteins; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014157:Transcription Factors
D004268:DNA-Binding Proteins; C492258:FUBP1 protein, human; C492259:FUBP3 protein, human; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D004265:DNA Helicases