title: [Sequence analysis of ITS2 and CO1 genes of Paragonimus harinasutai].
abstract: OBJECTIVE
  To identify Paragonimus harinasutai from Ninghai, Zhejiang Province, China.
  
  
  METHODS
  Metacercariae were collected from the crabs Sinopotamon chekiangenes in Xixi village of Ninghai County for ITS2 sequence analysis, CO1 sequence analysis and endonuclease BsaHI and StuI analysis by PCR-RFLP. Results The fingerprintings of PCR-RFLP were virtually same to the isolate from Thailand (Nakorn-nayok). The ITS2 sequence with 366 bp and CO1 sequence with 390 bp of the metacercariae collected from Ninghai revealed a nucleotide identity 95.6% and 89.5% respectively to the Thai isolate.
  
  
  CONCLUSIONS
  The study confirmed that Paragonimus harinasutai is present in Ninghai, China, with certain variation on molecular biology in comparison to the Thai isolate.
authors.affiliation: Institute of Bio-engineering, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
authors.forename: Bao-zhen
authors.initials: BZ
authors.lastname: Qian
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
authors.forename: J
authors.initials: J
authors.lastname: Waikagul
authors.forename: Zhi-hang
authors.initials: ZH
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D008969:Molecular Sequence Data; D010238:Paragonimus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV