title: Comparative biochemical study of N-linked glycans from skin of a squid, Todarodes pacificus.
abstract: For comparative biochemical interest, we analyzed the structures of N-glycans in a squid belonging to the Lophotrochozoa, one of the protostome clades. N-Glycans were prepared from squid skin by hydrazinolysis and re-N-acetylation followed by fluorescent tagging with 2-aminopyridine. The labeled N-glycans were purified, and their structures were determined by the two-dimensional HPLC mapping method combined with glycosidase digestions and mass spectrometry. We found that high mannose-type glycans, paucimannose-type glycans and complex-type glycans with a type-1 structure (Galbeta1-3GlcNAc) were dominant in squid skin. The complex-type glycans detected in the squid were similar to those in vertebrates, but have not yet been found in the Ecdysozoa, which is another protostome clade. However, paucimannose-type glycans are commonly found in the Ecdysozoa. Thus, the N-glycan structures of the squid belonging to the Lophotrochozoa have features common to those in vertebrates and the Ecdysozoa including insects and nematodes.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043. natsuka@chem.sci.osaka-u.ac.jp
authors.forename: Shunji
authors.initials: S
authors.lastname: Natsuka
authors.forename: Miwa
authors.initials: M
authors.lastname: Ishida
authors.forename: Akira
authors.initials: A
authors.lastname: Ichikawa
authors.forename: Koji
authors.initials: K
authors.lastname: Ikura
authors.forename: Sumihiro
authors.initials: S
authors.lastname: Hase
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D049832:Decapodiformes / Q000737:chemistry*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012867:Skin / Q000737:chemistry; D001616:beta-Galactosidase / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D001616:beta-Galactosidase; D001619:beta-N-Acetylhexosaminidases