Efficient hepatitis C antigen immunohistological staining in sections of normal, cirrhotic and tumoral liver using a new monoclonal antibody directed against serum-derived HCV E2 glycoproteins.
Detection and localization of Hepatitis C Virus (HCV) in liver tissue is useful for diagnostic purposes as well as to elucidate the mechanisms by which the virus participates in hepatocarcinogenesis. However, so far, a sensitive method for HCV detection at the cellular level is lacking. We describe here the application of a novel antibody, D4.12.9, developed against serum-derived HCV RNA-positive particles, for the detection of E2 proteins by immunohistochemistry in fixed, archived specimens including liver biopsies of HCV-infected patients and surgical specimens of hepatocellular carcinoma. We demonstrate that D4.12.9 is a powerful tool for sensitive and specific detection of HCV, independently of viral genotype. This approach has applications to diagnosis as well as exploratory pathological studies.
Galy
Oivier
O
INSERM U271, 151 Cours Albert Thomas, 69424 Lyon Cedex 03, France.
Petit
Marie-Anne
MA
Benjelloun
Soumaya
S
Chevallier
Philippe
P
Chevallier
Michelle
M
Srivatanakul
Petcharin
P
Karalak
Anant
A
Carreira
Christine
C
Lyandrat
Nicole
N
Essaid
Abdellah
A
Trepo
Christian
C
Hainaut
Pierre
P
Chemin
Isabelle
I
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D018936:Hepatitis C Antigens / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000821:virology*; D008103:Liver Cirrhosis / Q000175:diagnosis / Q000821:virology*; D008113:Liver Neoplasms / Q000175:diagnosis / Q000821:virology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000097:blood / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018936:Hepatitis C Antigens; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus