Analysis of neomycins A, B and C by high-performance liquid chromatography with post-column reaction detection.
The analysis of the antibiotics neomycins A, B and C was investigated. The separation of the components was studied using reversed-phase and reversed-phase ion-pair chromatography. The optimum separation was obtained utilizing a Lichrosorb RP-2 column with a mobile phase consisting of 75 mg/l sodium dodecyl sulphate, 0.5M Na2SO4 and 0.015 M sodium acetate buffer at pH 7.0. Using this mobile phase, baseline separation was obtained for all three compounds in approximately 20 min. Detection was via post-column derivatization of the analytes with ortho-phthalaldehyde in the presence of mercaptoethanol to form fluorescent iso-indole products. This system is applied to the analysis of a number of formulated products containing neomycin.
Apffel
J A
JA
Department of Analytical Chemistry, Free University, Amsterdam, The Netherlands.
Van Der Louw
J
J
Lammers
K R
KR
Kok
W T
WT
Brinkman
U A
UA
Frei
R W
RW
Burgess
C
C