title: Structure of Mycobacterium tuberculosis RuvA, a protein involved in recombination.
abstract: The process of recombinational repair is crucial for maintaining genomic integrity and generating biological diversity. In association with RuvB and RuvC, RuvA plays a central role in processing and resolving Holliday junctions, which are a critical intermediate in homologous recombination. Here, the cloning, purification and structure determination of the RuvA protein from Mycobacterium tuberculosis (MtRuvA) are reported. Analysis of the structure and comparison with other known RuvA proteins reveal an octameric state with conserved subunit-subunit interaction surfaces, indicating the requirement of octamer formation for biological activity. A detailed analysis of plasticity in the RuvA molecules has led to insights into the invariant and variable regions, thus providing a framework for understanding regional flexibility in various aspects of RuvA function.
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
authors.forename: J Rajan
authors.initials: JR
authors.lastname: Prabu
authors.forename: S
authors.initials: S
authors.lastname: Thamotharan
authors.forename: Jasbeer Singh
authors.initials: JS
authors.lastname: Khanduja
authors.forename: Emily Zabala
authors.initials: EZ
authors.lastname: Alipio
authors.forename: Chang Yub
authors.initials: CY
authors.lastname: Kim
authors.forename: Geoffrey S
authors.initials: GS
authors.lastname: Waldo
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Terwilliger
authors.forename: Brent
authors.initials: B
authors.lastname: Segelke
authors.forename: Tim
authors.initials: T
authors.lastname: Lekin
authors.forename: Dominique
authors.initials: D
authors.lastname: Toppani
authors.forename: Li Wei
authors.initials: LW
authors.lastname: Hung
authors.forename: Minmin
authors.initials: M
authors.lastname: Yu
authors.forename: Evan
authors.initials: E
authors.lastname: Bursey
authors.forename: K
authors.initials: K
authors.lastname: Muniyappa
authors.forename: Nagasuma R
authors.initials: NR
authors.lastname: Chandra
authors.forename: M
authors.initials: M
authors.lastname: Vijayan
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004265:DNA Helicases / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D011487:Protein Conformation; D011995:Recombination, Genetic
chemical_list: D001426:Bacterial Proteins; D004265:DNA Helicases