[Detection and kinetic characterization of Candida tropicalis contamination during fodder yeast production].
Candida tropicalis was identified as the etiologic agent of a severe contamination detected on an industrial fodder yeast production at the Cuban eastern region. After a detailed diagnostic task on raw material carried out on different factory sections, protocols to identify the contamination source and to isolate the microorganism were proposed. The identification was by comparison of the internal transcribed spacers ITS1 and ITS4 from 5.8S ribosomal DNA nucleotide sequences. In parallel, propagation of production strain, Candida utilis NRRL Y-660, at lab scale (2.5 l) was performed. Similar results to those observed in the factory concerning to its kinetic behavior in aerobic propagation with contaminated molasses, were detected at this level. The identification and primary kinetic characterization led to the implementation of sanitary and technological measures to bring production at its normal operational conditions as well as the application of prophylactic surveillance methodologies to avoid future contaminations.
García Gutiérrez
Roxana
R
Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar, Apartado postal 4026, La Habana, Cuba. roxana.garcia@icidca.edu.cu
Otero Rambla
Miguel Angel
MA
Fundora Toucet
Norka
N
Martínez Sánchez
Aidín
A
Saura Lauria
Gustavo
G
Casalot
Laurence
L
D000821:Animal Feed / Q000382:microbiology*; D001483:Base Sequence; D002175:Candida / Q000254:growth & development; D041022:Candida tropicalis / Q000235:genetics / Q000302:isolation & purification*; D003462:Cuba; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D005285:Fermentation; D005506:Food Contamination; D005516:Food Microbiology; D007218:Industrial Microbiology / Q000379:methods*; D007700:Kinetics; D008965:Molasses / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012340:RNA, Ribosomal, 5.8S / Q000032:analysis
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S