Determination of ibuprofen in human plasma by solid phase extraction and reversed-phase high-performance liquid chromatography.
A new, sensitive and simple method for the rapid quantitative determination of ibuprofen in human plasma has been developed. This method involves the use of a solid phase extraction on "Baker" C-18 disposable extraction columns for sample clean-up and uses mefenamic acid as an internal standard. Separation and quantitation are performed by reversed-phase liquid chromatography using a Nucleosil C18 column and methanol-0.04 M phosphoric acid (80:20, v/v), as the mobile phase. Detection was achieved by UV-absorbance measurements at 229 nm.
Jonkman
J H
JH
Pharma Bio-Research International B. V., Department of Biopharmaceutics and Clinical Pharmacokinetics, P.O. Box 147, 9400 AC Assen, The Netherlands.
Schoenmaker
R
R
Holtkamp
A H
AH
Hempenius
J
J