title: The change of protein intradomain mobility on ligand binding: is it a commonly observed phenomenon?
abstract: Analysis of changes in the dynamics of protein domains on ligand binding is important in several aspects: for the understanding of the hierarchical nature of protein folding and dynamics at equilibrium; for analysis of signal transduction mechanisms triggered by ligand binding, including allostery; for drug design; and for construction of biosensors reporting on the presence of target ligand in studied media. In this work we use the recently developed HCCP computational technique for the analysis of stabilities of dynamic domains in proteins, their intrinsic motions and of their changes on ligand binding. The work is based on comparative studies of 157 ligand binding proteins, for which several crystal structures (in ligand-free and ligand-bound forms) are available. We demonstrate that the domains of the proteins presented in the Protein DataBank are far more robust than it was thought before: in the majority of the studied proteins (152 out of 157), the ligand binding does not lead to significant change of domain stability. The exceptions from this rule are only four bacterial periplasmic transport proteins and calmodulin. Thus, as a rule, the pattern of correlated motions in dynamic domains, which determines their stability, is insensitive to ligand binding. This rule may be the general feature for a vast majority of proteins.
authors.affiliation: Department of Physics of Biological Systems, Institute of Physics, National Academy of Science of Ukraine, Kiev, Ukraine. yesint3@yahoo.com
authors.forename: Semen O
authors.initials: SO
authors.lastname: Yesylevskyy
authors.forename: Valery N
authors.initials: VN
authors.lastname: Kharkyanen
authors.forename: Alexander P
authors.initials: AP
authors.lastname: Demchenko
mesh_terms: D015374:Biosensing Techniques; D003198:Computer Simulation; D008024:Ligands; D008958:Models, Molecular; D009038:Motion; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*
chemical_list: D008024:Ligands; D011506:Proteins