title: Quantitative analysis of cutaneous malassezia in atopic dermatitis patients using real-time PCR.
abstract: We quantified the cutaneous Malassezia in patients with atopic dermatitis using a real-time PCR assay. Seven to 12 times more Malassezia colonized the head and neck compared to the trunk or limbs, and the species M. globosa and M. restricta accounted for approximately 80% of all Malassezia colonization at any body site.
authors.affiliation: Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. sugita@my-pharm.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugita
authors.forename: Mami
authors.initials: M
authors.lastname: Tajima
authors.forename: Hisae
authors.initials: H
authors.lastname: Tsubuku
authors.forename: Ryoji
authors.initials: R
authors.lastname: Tsuboi
authors.forename: Akemi
authors.initials: A
authors.lastname: Nishikawa
mesh_terms: D000328:Adult; D003876:Dermatitis, Atopic / Q000382:microbiology*; D003881:Dermatomycoses / Q000382:microbiology*; D005260:Female; D006801:Humans; D008290:Malassezia / Q000254:growth & development / Q000302:isolation & purification*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods