Comparison of Dynabeads and Capcellia methods with FACScount for the estimation of CD4 T-lymphocyte levels in HIV/AIDS patients in Lagos, Nigeria.
OBJECTIVE
To compare the efficacy of the Capcellia assay and the Dynabeads technique against the FACScount technique in the estimation of CD4 T-lymphocytes within a Nigerian setting.


METHODS
Prospective study.


METHODS
Urban area in Nigeria.


METHODS
Ninety seven subjects (51 HIV seronegative and 46 HIV seropositive adults) seen in the blood banks and two out patient clinics in Lagos within the study period.


METHODS
Dynadeads technique with a higher correlation coefficient is a feasible alternative to the FACScount method.


RESULTS
There was an overall correlation coefficient of r = 0.75 for CD4 cell counts as determined by the Dynabeads technique in comparison with the FACScount method. Also, an overall correlation coefficient of r = 0.17 for CD4 cell counts as determined by the Capcellia technique against the FACScount method.


CONCLUSIONS
Dynabeads technique is simple to carry out and cheaper in terms of demand for human expertise and infrastructural requirements than the FACScounts. Therefore, it was recommended for use in the laboratory for monitoring of ARV therapy in Nigeria and any other resource poor
Idigbe
E O
EO
Nigerian Institute of Medical Research, P.M.B 2013, Yaba, Lagos, Nigeria.
Audu
R A
RA
Oparaugo
C T
CT
Onwujekwe
D
D
Onubogu
C C
CC
Adedoyin
J
J
Mafe
A G
AG
Okoye
R
R
Onyewuche
J I
JI
Funso-Adebayo
E O
EO
Meshack
E
E
D000293:Adolescent; D000328:Adult; D018791:CD4 Lymphocyte Count / Q000379:methods; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D003365:Costs and Cost Analysis; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006679:HIV Seropositivity / Q000276:immunology*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D008297:Male; D009549:Nigeria / Q000453:epidemiology; D010865:Pilot Projects; D011446:Prospective Studies; D012680:Sensitivity and Specificity