Diffusion of single star-branched dendrimer-like DNA.
We report a single-molecule fluorescence study on the diffusion of star-branched polymer dendrimer-like DNA (DL-DNA). The DL-DNA molecules were synthesized from ligating Y-shaped DNA to the fourth generation. It was found through single particle tracking that the diffusion coefficient of DL-DNA changes in a nonmonotonic fashion with its increased concentration possibly due to arm arrest and arm retraction. The diffusion of DL-DNA in linear lambda DNA solution displayed a monotonic concentration dependence 1 order of magnitude greater than the diffusion of DL-DNA in DL-DNA solution. This difference is attributed to the different conformation of DL-DNA and lambda DNA and the entanglement of lambda DNA with a large radius of gyration. Our diffusion study facilitates DL-DNA transportation for drug delivery.
Freedman
Katherine O
KO
Department of Physics and Astronomy, Clemson University, Clemson, South Carolina 29634, USA.
Lee
Janet
J
Li
Yougen
Y
Luo
Dan
D
Skobeleva
Victoria B
VB
Ke
Pu Chun
PC
D001583:Benzoxazoles; D004396:Coloring Agents; D004247:DNA / Q000737:chemistry*; D003627:Data Interpretation, Statistical; D050091:Dendrimers / Q000737:chemistry*; D004058:Diffusion; D016503:Drug Delivery Systems; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation
D001583:Benzoxazoles; D004396:Coloring Agents; D050091:Dendrimers; C120457:POPO 3; D004247:DNA