Global changes in cellular gene expression during bacteriophage PRD1 infection.
Virus-induced changes in cellular gene expression and host physiology have been studied extensively. Still, there are only a few analyses covering the entire viral replication cycle and whole-host gene pool expression at the resolution of a single gene. Here we report changes in Escherichia coli gene expression during bacteriophage PRD1 infection using microarray technology. Relative mRNA levels were systematically measured for over 99% of the host open reading frames throughout the infection cycle. Although drastic modifications could be detected in the expression of individual genes, global changes at the whole-genome level were moderate. Notably, the majority of virus-induced changes took place only after the synthesis of virion components, indicating that there is no major reprogramming of the host during early infection. The most highly induced genes encoded chaparones and other stress-inducible proteins.
Poranen
Minna M
MM
Institute of Biotechnology, Viikki Biocenter, P.O. Box 56 (Viikinkaari 5), 00014 University of Helsinki, Helsinki, Finland.
Ravantti
Janne J
JJ
Grahn
A Marika
AM
Gupta
Rashi
R
Auvinen
Petri
P
Bamford
Dennis H
DH
D025622:Bacteriophage PRD1 / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics* / Q000821:virology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D014771:Virion / Q000378:metabolism; D014779:Virus Replication
D011135:Polysaccharides, Bacterial; D012333:RNA, Messenger