title: Identification of E2/E3 ubiquitinating enzymes and caspase activity regulating Drosophila sensory neuron dendrite pruning.
abstract: Ubiquitin-proteasome system (UPS) is a multistep protein degradation machinery implicated in many diseases. In the nervous system, UPS regulates remodeling and degradation of neuronal processes and is linked to Wallerian axonal degeneration, though the ubiquitin ligases that confer substrate specificity remain unknown. Having shown previously that class IV dendritic arborization (C4da) sensory neurons in Drosophila undergo UPS-mediated dendritic pruning during metamorphosis, we conducted an E2/E3 ubiquitinating enzyme mutant screen, revealing that mutation in ubcD1, an E2 ubiquitin-conjugating enzyme, resulted in retention of C4da neuron dendrites during metamorphosis. Further, we found that UPS activation likely leads to UbcD1-mediated degradation of DIAP1, a caspase-antagonizing E3 ligase. This allows for local activation of the Dronc caspase, thereby preserving C4da neurons while severing their dendrites. Thus, in addition to uncovering E2/E3 ubiquitinating enzymes for dendrite pruning, this study provides a mechanistic link between UPS and the apoptotic machinery in regulating neuronal process remodeling.
authors.affiliation: Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: Chay T
authors.initials: CT
authors.lastname: Kuo
authors.forename: Sijun
authors.initials: S
authors.lastname: Zhu
authors.forename: Susan
authors.initials: S
authors.lastname: Younger
authors.forename: Lily Y
authors.initials: LY
authors.lastname: Jan
authors.forename: Yuh Nung
authors.initials: YN
authors.lastname: Jan
mesh_terms: D000818:Animals; D020169:Caspases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D003712:Dendrites / Q000737:chemistry / Q000201:enzymology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009475:Neurons, Afferent / Q000737:chemistry / Q000201:enzymology*; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D044763:Ubiquitin-Conjugating Enzymes; D044767:Ubiquitin-Protein Ligases; D020169:Caspases; C118731:dronc protein, Drosophila