title: Neuroproteomics: relevance to anxiety disorders.
abstract: Despite advances in the treatment of anxiety disorders, there is a need for medications with greater efficacy and fewer side effects. Advances in techniques to facilitate high throughput, mass analysis of proteins potentially allows for new drug targets, with a shift in focus from membrane receptor proteins and enzymes of neurotransmitter metabolism to molecules in intracellular signal transduction and other pathways. A computerized literature search was done to collect studies on recently developed proteomic techniques and their application in psychiatric research. Particular techniques, such as two-dimensional electrophoresis, two-dimensional differential gel electrophoresis, isotope-coded affinity tags, and isotope tags for relative and absolute quantification, are reviewed. In addition, a combination of these techniques with MALDI-TOF/TOF and ESI-Q-TOF mass spectrometry analysis is discussed in relation to possible novel signaling pathways relevant to anxiety disorders, and to the development of biomarkers for the evaluation of these conditions.
authors.affiliation: MRC Unit for Stress and Anxiety Disorders, Laboratory for Neuroproteomics, Department of Biomedical Sciences, Division of Medical Physiology, University of Stellenbosch, Tygerberg, South Africa. jduys@sun.ac.za
authors.forename: Joachim D K
authors.initials: JD
authors.lastname: Uys
authors.forename: Dan J
authors.initials: DJ
authors.lastname: Stein
authors.forename: Willie M U
authors.initials: WM
authors.lastname: Daniels
mesh_terms: D000818:Animals; D001008:Anxiety Disorders / Q000175:diagnosis* / Q000503:physiopathology*; D001921:Brain / Q000503:physiopathology*; D004195:Disease Models, Animal; D004586:Electrophoresis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D001523:Mental Disorders / Q000175:diagnosis* / Q000503:physiopathology*; D040901:Proteomics / Q000379:methods*