Diagnosis of human rotavirus in stool specimens: comparison of different methods.
A total of 345 stool specimens of hospitalized children below 5 years of age with acute gastroenteritis were tested for the presence of rotavirus by Polyacrylamide gel electrophoresis (PAGE), a monoclonal antibody based enzyme immunoassay (EIA) and a latex agglutination test (LAT). Detection rate for PAGE and EIA were 24.9% (345/86) and 20.9% (345/70) respectively. Using PAGE as the standard, the sensitivity and specificity of EIA were 75.6% and 98.1% respectively. The sensitivity of LAT was 70.9% with 100% specificity (LAT was done in only PAGE positive specimens). LAT appeared as the simplest and economic for both bed side and field use.
Paul
S K
SK
Department of Microbiology, Mymensingh Medical College.
Tabassum
S
S
Islam
M N
MN
Ahmed
M U
MU
Haq
J U
JU
Shamsuzzaman
A K
AK
D001459:Bangladesh / Q000453:epidemiology; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D007841:Latex Fixation Tests; D008297:Male; D015995:Prevalence; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000453:epidemiology; D012680:Sensitivity and Specificity