title: Genetic involvement in skin wound healing and scarring in domestic pigs: assessment of molecular expression patterns in (Yorkshire x Red Duroc) x Yorkshire backcross animals.
abstract: Yorkshire, red Duroc, and F1 (first-generation cross) pigs heal with normal, fibroproliferative/hypercontractile, and intermediate levels of scarring, respectively. The purpose of this study was to evaluate the healing phenotype of Yorkshire x F1 backcross animals, to address the molecular basis for genetic transmission of the red Duroc scarring phenotype. Macroscopically and histologically, full-thickness wounds on backcross animals followed the Yorkshire phenotype, with one exception; the backcross wounds exhibited contraction following re-epithelialization. The molecular expression patterns in the backcross animals generally correlated with the macroscopic and histologic findings. Compared to Yorkshire, red Duroc, and F1 wounds, the backcross wounds demonstrated a diminished initial inflammatory phase, followed by a prolonged expression of several relevant growth factors. Additionally, collagen expression was prolonged, expression of matrix metalloproteinases was increased, and alterations in tissue inhibitor of metalloproteinase expression were detected. Moreover, a subset of molecules still followed the red Duroc pattern of mRNA expression, a finding that allows for correlations between the scarring phenotype and the molecular expression patterns to be made in this model. The results indicate that a number of genes are likely involved in the red Duroc healing phenotype and that identification of the specific genes involved will require a more detailed genomic analysis.
authors.affiliation: Department of Surgery, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Corrie L
authors.initials: CL
authors.lastname: Gallant-Behm
authors.forename: Carol
authors.initials: C
authors.lastname: Reno
authors.forename: Helen
authors.initials: H
authors.lastname: Tsao
authors.forename: David A
authors.initials: DA
authors.lastname: Hart
mesh_terms: D000818:Animals; D002921:Cicatrix / Q000235:genetics* / Q000473:pathology; D003094:Collagen / Q000235:genetics; D003433:Crosses, Genetic; D016207:Cytokines / Q000235:genetics; D005260:Female; D051523:Fibroblast Growth Factor 7 / Q000235:genetics; D020869:Gene Expression Profiling; D020782:Matrix Metalloproteinases / Q000235:genetics; D053495:Osteopontin / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D016212:Transforming Growth Factor beta / Q000235:genetics; D014945:Wound Healing / Q000235:genetics*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D053495:Osteopontin; D051523:Fibroblast Growth Factor 7; D003094:Collagen; D020782:Matrix Metalloproteinases