title: Bioactive glass nanofiber-collagen nanocomposite as a novel bone regeneration matrix.
abstract: Nanoscale organized organic-inorganic nanocomposite systems have great potential in the development of biomaterials with advanced properties. Herein, we developed a novel nanocomposite biomaterial consisting of bioactive glass nanofiber (BGNF) and collagen reconstituted fibrous matrix for bone regenerative medicine. A sol-gel derived glass with a bioactive composition (58SiO(2). 38CaO.4P(2)O(5)) was electrospun to a nanoscale fiber with an average diameter of approximately 320 nm. The BGNF was subsequently hybridized with type I collagen, which is the main organic constituent of bone matrix. The BGNF and self-assembled collagen sol were combined in aqueous solution, and then crosslinked to produce a BGNF-collagen nanocomposite, in the form of either a thin membrane or a macroporous scaffold, by adopting appropriate processing conditions. The BGNF was observed to be distributed uniformly within the collagen reconstituted nanofibrous matrix. The nanocomposite matrices induced rapid formation of bone-like apatite minerals on their surfaces when incubated in a simulated body fluid, exhibiting excellent bioactivity in vitro. Osteoblastic cells showed favorable growth on the BGNF-collagen nanocomposite. In particular, the alkaline phosphatase activity of the cells on the nanocomposite was significantly higher than that on the collagen. This novel BGNF-collagen nanocomposite is believed to have significant potential in bone regeneration and tissue engineering applications.
authors.affiliation: Department of Dental Biomaterials, School of Dentistry, Dankook University, Cheonan 330-714, Korea. kimhw@dku.edu
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
authors.forename: Ju-Ha
authors.initials: JH
authors.lastname: Song
authors.forename: Hyoun-Ee
authors.initials: HE
authors.lastname: Kim
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001857:Bone Matrix / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*; D001861:Bone Regeneration / Q000187:drug effects*; D002460:Cell Line; D048430:Cell Shape; D003094:Collagen / Q000494:pharmacology* / Q000648:ultrastructure*; D005898:Glass; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D003094:Collagen