Analysis of cell-based RNAi screens.
RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (http://www.dkfz.de/signaling/cellHTS). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.
Boutros
Michael
M
Signaling and Functional Genomics, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany. m.boutros@dkfz.de
Brás
Lígia P
LP
Huber
Wolfgang
W
D011786:Quality Control; D034622:RNA Interference; D015203:Reproducibility of Results