title: Expression, purification, crystallization and preliminary X-ray crystallographic studies of Deinococcus radiodurans thioredoxin reductase.
abstract: Deinococcus radiodurans, a Gram-positive bacterium capable of withstanding extreme ionizing radiation, contains two thioredoxins (Trx and Trx1) and a single thioredoxin reductase (TrxR) as part of its response to oxidative stress. Thioredoxin reductase is a member of the family of pyridine nucleotide-disulfide oxidoreductase flavoenzymes. Recombinant D. radiodurans TrxR with a His tag at the N-terminus was expressed in Escherichia coli and purified by metal-affinity chromatography. The protein was crystallized using the sitting-drop vapour-diffusion method in the presence of 35% PEG 4000, 0.2 M ammonium acetate and citric acid buffer pH 5.1 at 293 K. X-ray diffraction data were collected on a cryocooled crystal to a resolution of 1.9 angstroms using a synchrotron-radiation source. The space group was determined to be P3(2)21, with unit-cell parameters a = b = 84.33, c = 159.88 angstroms. The structure of the enzyme has been solved by molecular-replacement methods and structure refinement is in progress.
authors.affiliation: Department of Chemistry, University of Saskatchewan, Saskatoon, SK, S7N 5C9, Canada.
authors.forename: Josiah
authors.initials: J
authors.lastname: Obiero
authors.forename: Sara A
authors.initials: SA
authors.lastname: Bonderoff
authors.forename: Meghan M
authors.initials: MM
authors.lastname: Goertzen
authors.forename: David A R
authors.initials: DA
authors.lastname: Sanders
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D034301:Deinococcus / Q000201:enzymology*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013880:Thioredoxin-Disulfide Reductase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D013880:Thioredoxin-Disulfide Reductase