title: [Determination of alkaloids in Radix Aconiti Lateralis Preparata by RP-ion-pair HPLC].
abstract: OBJECTIVE
  To separate and quantitatively determine six alkaloids: aconitine, mesaconitine, hypaconitine, beiwutine, benzoylaconine and benzoylmesaconine in the Chinese traditional medicine Radix Aconiti Lateralis Preparata (Fuzi).
  
  
  METHODS
  A RP-ion-pair HPLC method was established. An AichromBond-1 C18 column was used at a column-temperature of 35 degrees C. The mobile phase was CH3CN5 mmol x L(-1) NaH2PO4(50:50) containing 7 mmol x L(-1) SDS at a flow-rate of 1.0 mL x min(-1). The detector was set at UV 235 nm.
  
  
  RESULTS
  These six alkaloids can be completely separated and determined quantitatively.
  
  
  CONCLUSIONS
  This method is accurate and suitable for the determination of six alkaloids in Fuzi.
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu.
authors.forename: Xiu-xiu
authors.initials: XX
authors.lastname: Liu
authors.forename: Ruo-bing
authors.initials: RB
authors.lastname: Chao
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification*; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000470:Alkaloids; C047307:benzoylaconine; C085283:benzoylmesaconine; D000157:Aconitine