Kinetics of n-butyl benzyl phthalate degradation by a pure bacterial culture from the mangrove sediment.
n-Butyl benzyl phthalate (BBP) is an endocrine-disrupting chemical. Biodegradation of BBP by a bacterium, Pseudomonas fluorescens B-1, isolated from the mangrove sediment, was investigated. The microorganism can utilize BBP as the sole carbon and energy source, where concentrations of BBP disappeared within 6 days under shake culture conditions. Effects of BBP concentration, pH, temperature, and salinity on BBP biodegradation were studied, respectively. The process of BBP biodegradation was monitored by reversed-phase high-performance liquid chromatography with ultra-violet detection after solid-phase extraction. The biodegradation of BBP could be fitted to a first-order kinetic model. The major metabolites of BBP degradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid and benzoic acid by gas chromatography-mass spectrometry, and a preliminary metabolic pathway of BBP was proposed.
Xu
Xiang-Rong
XR
Department of Ecology & Biodiversity, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, PR China. xrxu@hkucc.khu.hk
Li
Hua-Bin
HB
Gu
Ji-Dong
JD
Li
Xiao-Yan
XY
D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D019015:Geologic Sediments; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009994:Osmolar Concentration; D010795:Phthalic Acids / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D043982:Rhizophoraceae / Q000382:microbiology*; D012988:Soil Microbiology; D013696:Temperature
D010795:Phthalic Acids; C027561:butylbenzyl phthalate