Calcium salicylate-mediated apoptosis in human HT-1080 fibrosarcoma cells.
Salicylates are novel biologically active compounds that exhibit multiple therapeutic activities. The anti-cancer effectiveness of calcium salicylate has been investigated on human HT-1080 fibrosarcoma cell lines at relatively low concentrations (predominantly 0.4 mM) compared to those previously reported. Although low calcium salicylate concentrations did not retard tumour growth progression significantly, as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and time-lapse assays, its cytotoxic characteristics were proven to be prominent by various morphological and immunocytological techniques. The results here demonstrate evidence for approximately 25% apoptosis after treatment with calcium salicylate, which up-regulatd the expression of p53, p21 and Bax, and down-regulated Bcl-2 in HT-1080 cells.
Mahdi
J G
JG
School of Biosciences, Cardiff University, PO Box 911, Cardiff CF10 3US, UK. mahdijg@cf.ac.uk
Alkarrawi
M A
MA
Mahdi
A J
AJ
Bowen
I D
ID
Humam
D
D
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D005354:Fibrosarcoma; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007202:Indicators and Reagents; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D012459:Salicylates / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis; D054462:p21-Activated Kinases
D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D019253:Proto-Oncogene Proteins c-bcl-2; D012459:Salicylates; D013778:Tetrazolium Salts; D013844:Thiazoles; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C513091:calcium salicylate; D017346:Protein Serine-Threonine Kinases; D054462:p21-Activated Kinases; C022616:thiazolyl blue