alpha-tocopherol supplementation reduces the elevated 8-hydroxy-2-deoxyguanosine blood levels induced by training in basketball players.
BACKGROUND
The aim of the present study was to investigate the effect of alpha-tocopherol (alpha-Te) supplementation on DNA oxidative damage induced by heavy training in basketball players.


METHODS
Blood was obtained from 10 players before (group A) and after training (group B) and after 1 month on alpha-Te (200 mg/day, orally) supplementation, before (group C) and after training (group D). Total antioxidant status (TAS), muscle enzyme activities and the biomarker of DNA oxidation, 8-hydroxy-2-deoxyguanosine (8-OHdG), were measured using commercial kits. alpha-Te and catecholamine blood levels were determined using HPLC methods.


RESULTS
TAS was higher in the groups with alpha-Te (groups C and D). Levels of 8-OHdG and muscle creatine kinase (CK) and lactate dehydrogenase (LDH) were remarkably lower (0.20+/-0.03 ng/mL, 120+/-15 U/L and 430+/-90 U/L, respectively) in the group with alpha-Te (group D) than in group B (0.42+/-0.05 ng/mL, 286+/-12 U/L and 688+/-88 U/L, respectively; p<0.001). 8-OHdG levels were negatively correlated to TAS and positively to CK levels.


CONCLUSIONS
alpha-Te supplementation may reduce DNA oxidation induced by training by protecting muscle cell "death" from glutamate entry and/or by elevation of TAS via amelioration of lipid peroxidation.
Tsakiris
Stylianos
S
Department of Experimental Physiology, Medical School, University of Athens, Athens, Greece. stsakir@cc.uoa.gr
Parthimos
Theodore
T
Tsakiris
Theodore
T
Parthimos
Nickolaos
N
Schulpis
Kleopatra H
KH
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage* / Q000378:metabolism; D001490:Basketball / Q000502:physiology*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000097:blood; D019587:Dietary Supplements; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D013663:Teaching; D024502:alpha-Tocopherol / Q000008:administration & dosage* / Q000097:blood
D000975:Antioxidants; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; D024502:alpha-Tocopherol