title: Transient ischemia-induced expression and changes of tyrosine kinase A in the hippocampal dentate gyrus of the gerbil.
abstract: The present study examined ischemia-related changes in tyrosine kinase A (trkA) immunoreactivity and its protein content in the dentate gyrus after 5 min of transient forebrain ischemia in gerbils. One day after ischemic insult, cresyl violet-positive polymorphic cells showed ischemic degeneration. The ischemia-induced changes in trkA immunoreactivity were found in the polymorphic layer (PL) and granule cell layer (GCL) of the dentate gyrus. In the sham-operated group, trkA immunoreactivity in the dentate gyrus was very weak. From 30 min after ischemia, trkA immunoreactivity was increased in the dentate gyrus and peaked in the dentate gyrus at 12 h after ischemia-reperfusion. Thereafter, trkA immunoreactivity was decreased time-dependently after ischemia-reperfusion. Four days after ischemic insult, trkA immunoreactivity was similar to that of the sham-operated group. In addition, it was found that ischemia-related changes in trkA protein content were similar to the immunohistochemical changes. These results suggest that the chronological changes of trkA in the dentate gyrus after transient forebrain ischemia may be associated with ischemic damage in polymorphic cells of the dentate gyrus.
authors.affiliation: Department of Anatomy, College of Medicine Hallym University, Chunchon, South Korea.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Hyeon Yong
authors.initials: HY
authors.lastname: Lee
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Dae Ho
authors.initials: DH
authors.lastname: Kim
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Kim
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Beong Ou
authors.initials: BO
authors.lastname: Lim
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Kyung Hwan
authors.initials: KH
authors.lastname: Bang
authors.forename: Nak Sul
authors.initials: NS
authors.lastname: Seong
authors.forename: Hak Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jong Dai
authors.initials: JD
authors.lastname: Kim
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D005849:Gerbillinae; D007150:Immunohistochemistry / Q000379:methods; D002546:Ischemic Attack, Transient / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D020534:Parahippocampal Gyrus / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011505:Protein-Tyrosine Kinases