title: Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample.
abstract: Two multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101-DXS7424 and DXS6789-DXS6801-DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man.
authors.affiliation: Department of Anatomy, Pharmacology and Legal Medicine, University of Turin, c.so Galileo Galilei 22, 10126, Turin, Italy. carlo.robino@unito.it
authors.forename: C
authors.initials: C
authors.lastname: Robino
authors.forename: A
authors.initials: A
authors.lastname: Giolitti
authors.forename: S
authors.initials: S
authors.lastname: Gino
authors.forename: C
authors.initials: C
authors.lastname: Torre
mesh_terms: D041321:Chromosomes, Human, X / Q000235:genetics*; D005260:Female; D005828:Genetics, Population / Q000379:methods*; D006801:Humans; D007558:Italy; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction