Evaluation of respiratory syncytial virus detection by rapid antigen tests in childhood.
BACKGROUND
Rapid and reliable diagnosis is crucial for clinical management of respiratory syncytial virus infection in childhood. We assessed the performance characteristics of respiratory syncytial virus antigen immunoassays in children hospitalized for respiratory infection.


METHODS
A total of 1600 children up to three years of age hospitalized for diseases potentially caused by RSV were included in the study. Nasopharyngeal secretions were obtained in a standardized manner in the first 24 hours after hospital admission and tested in parallel by PCR and rapid antigen tests for RSV. The following parameters were recorded: recruitment center, gender, age, presence of fever, rhinitis, stridor, barking cough, cough, wheezing, vomiting, disturbed eating or sleep, tachypnea, tachycardia, increased body temperature, decreased oxygen saturation, C-reactive protein, erythrocyte sedimentation rate and chest X-ray results.


RESULTS
Considering PCR testing as gold standard, rapid antigen testing had a specificity of 89.9% and a sensitivity of 66.2% for all samples tested. Logistic regression analysis revealed age and recruitment center as the only parameters influencing sensitivity whereas no such influence on specificity was found. Positive likelihood ratios ranged from 4,9 to 6.9 in different age groups. Negative likelihood ratio was 0.24 (95% CI: 0.18-0.42) in children aged up to 3 months but 0.67 (95% CI: 0.53-0.84) for children older than 2 years.


CONCLUSIONS
Rapid detection of RSV antigen in this study was useful in detection of RSV mediated disease in younger infants but shows decreasing sensitivity in children older than three months.
Schauer
U
U
St. Josef Hospital, Klinik für Kinder- und Jugendmedizin, Ruhr-Universität Bochum. Uwe.Schauer@ruhr-uni-bochum.de
Ihorst
G
G
Rohwedder
A
A
Petersen
G
G
Berner
R
R
Frank
H D
HD
Forster
J
J
Stephan
V
V
D000956:Antigens, Viral / Q000097:blood*; D002675:Child, Preschool; D015331:Cohort Studies; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D007251:Influenza, Human / Q000175:diagnosis / Q000276:immunology; D008297:Male; D009305:Nasopharynx / Q000821:virology; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000276:immunology; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011446:Prospective Studies; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000276:immunology; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000276:immunology
D000956:Antigens, Viral