[Study on the methods for detection of Toxoplasma antigen].
A sandwich ELISA was established with monoclonal antibody as detecting antibody and rabbit anti-Toxoplasma polyclonal antibody as capturing antibody. ABC (avidin-biotin-peroxidase complex)-ELISA and immuno-PCR were also used to detect different concentrations of Toxoplasma antigen. The lowest concentration of antigen to be detected by sandwich ELISA, ABC-ELISA and immuno-PCR was 0. 6 microg/ml, 0. 075 microg/ml and 0.1 ng/ml respectively. The immuno-PCR shows a much higher sensitivity than the other two methods.
Li
Hui
H
Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450003, China.
Xu
Bian-li
BL
Deng
Yan
Y
Zhao
Xu-dong
XD
Lin
Xi-meng
XM
Yan
Xu-xia
XX
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D014122:Toxoplasma / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan