title: The role of aquaporin water channels in fluid secretion by the exocrine pancreas.
abstract: The mammalian exocrine pancreas secretes a near-isosmotic fluid over a wide osmolarity range. The role of aquaporin (AQP) water channels in this process is now becoming clearer. AQP8 water channels, which were initially cloned from rat pancreas, are expressed at the apical membrane of pancreatic acinar cells and contribute to their osmotic permeability. However, the acinar cells secrete relatively little fluid and there is no obvious defect in pancreatic function in AQP8 knockout mice. Most of the fluid secreted by the pancreas is generated by ductal epithelial cells, which comprise only a small fraction of the gland mass. In the human pancreas, secretion occurs mainly in the intercalated ducts, where the epithelial cells express abundant AQP1 and AQP5 at the apical membrane and AQP1 alone at the basolateral membrane. In the rat and mouse, fluid secretion occurs mainly in the interlobular ducts where AQP1 and AQP5 are again co-localized at the apical membrane but appear to be expressed at relatively low levels. Nonetheless, the transepithelial osmotic permeability of rat interlobular ducts is sufficient to support near-isosmotic fluid secretion at observed rates. Furthermore, apical, but not basolateral, application of Hg(2+) significantly reduces the transepithelial osmotic permeability, suggesting that apical AQP1 and AQP5 may contribute significantly to fluid secretion. The apparently normal fluid output of the pancreas in AQP1 knockout mice may reflect the presence of AQP5 at the apical membrane.
authors.affiliation: Molecular Oral Biology Research Group, Department of Oral Biology, Hungarian Academy of Sciences and Semmelweis University, Budapest, Hungary.
authors.forename: B
authors.initials: B
authors.lastname: Burghardt
authors.forename: S
authors.initials: S
authors.lastname: Nielsen
authors.forename: M C
authors.initials: MC
authors.lastname: Steward
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D045604:Extracellular Fluid / Q000378:metabolism*; D006801:Humans; D008628:Mercury / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D009995:Osmosis / Q000187:drug effects / Q000502:physiology; D046790:Pancreas, Exocrine / Q000166:cytology / Q000378:metabolism*; D010183:Pancreatic Ducts / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology*
chemical_list: D020346:Aquaporins; D008628:Mercury