Carbohydrate-based switch-on molecular sensor for Cu(II) in buffer: absorption and fluorescence study of the selective recognition of Cu(II) ions by galactosyl derivatives in HEPES buffer.
[graph: see text] 1-(Beta-D-galactopyranosyl-1'-deoxy-1'-iminomethyl)-2-hydroxynaphthalene (L1), possessing an ONO binding core, was found to be selective for Cu2+ ions in N-[2-hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid] buffer, at concentrations < or = 580 ppb, at physiological pH by eliciting switch-on behavior, whereas the other ions, viz., Mg2+, Ca2+, Mn2+, Fe2+, Co2+, Ni2+, Zn2+, and Cd2+, caused no significant change in the fluorescence. Whereas the binding characteristics were ascertained by absorption spectroscopy, the species formed were shown by Q-TOF ES MS.
Singhal
Nitin Kumar
NK
Bioinorganic Laboratory, Department of Chemistry, Indian Institute of Technology Bombay, Mumbai 400 076, India.
Ramanujam
Balaji
B
Mariappanadar
Vairamani
V
Rao
Chebrolu Pulla
CP
D002412:Cations / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D005453:Fluorescence; D005690:Galactose / Q000031:analogs & derivatives* / Q000737:chemistry*; D006531:HEPES; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry; D015394:Molecular Structure
D002412:Cations; D008670:Metals; D003300:Copper; D006531:HEPES; D005690:Galactose