N-hexacosanol ameliorates streptozotocin-induced diabetic rat nephropathy.
In this study we investigated the effects of N-hexacosanol on streptozotocin-induced rat diabetic nephropathy. Diabetes was induced in 8-week-old male Sprague-Dawley rats by administering an intraperitoneal injection of streptozotocin (50 mg/kg). The rats were divided into four groups and maintained for 8 weeks: control rats, diabetic rats without treatment with N-hexacosanol, and diabetic rats treated with N-hexacosanol (2 mg/kg and 8 mg/kg i.p. every day). Although N-hexacosanol failed to modify the diabetic status, increases in serum creatinine as well as in kidney weight were significantly reduced. The malonaldehyde and transforming growth factor beta-1 (TGF-beta1) concentrations as well as the protein kinase C (PKC) activities in the diabetic kidney were significantly higher than those of the control, which were decreased by treatment with N-hexacosanol. Histological examinations revealed that N-hexacosanol significantly ameliorated diabetic-induced tubulointerstitial pathological changes. Our data suggest that N-hexacosanol could prevent increases in the malonaldehyde and TGF-beta1 concentrations and PKC activities in the kidney, and ameliorate diabetic-induced nephropathy.
Saito
Motoaki
M
Department of Pathophysiological and Therapeutic Science, Division of Molecular Pharmacology, Tottori University Faculty of Medicine, 86 Nishimachi, Yonago 683-8503, Japan. saitomo@grape.med.tottori-u.ac.jp
Kinoshita
Yukako
Y
Satoh
Itaru
I
Shinbori
Chiko
C
Kono
Tomoharu
T
Hanada
Takuya
T
Uemasu
Jiro
J
Suzuki
Hiroto
H
Yamada
Masashi
M
Satoh
Keisuke
K
D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000139:chemically induced* / Q000473:pathology*; D005233:Fatty Alcohols / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D005233:Fatty Alcohols; D007328:Insulin; D053773:Transforming Growth Factor beta1; D008315:Malondialdehyde; D013311:Streptozocin; D003404:Creatinine; D011493:Protein Kinase C; C051942:1-hexacosanol