title: Effects of bio-active ceramic resources in cutaneous wound healing and the role of TGF-beta signaling.
abstract: The wound healing process is a highly orchestrated process, which includes inflammation, re-epithelialization, granulation tissue formation, matrix formation and re-modeling. In this paper, we attempt to determine if bio-active ceramic resource powder particles had an effect on cutaneous wound healing. Furthermore, we investigated its related mechanism and the expression of Smads of cutaneous wound healing, which can be accelerated by bio-active ceramic ointment. Topically applied lesions of 5%, 10% and 15% bio-active ceramic ointment (AO) showed accelerated wound closure, re-epithelialization, and the related immediate down stream of TGF-beta (p-Smad2/3 and Smad3) was suppressed. In particular, 10% and 15% AO lesions became closed faster at Days 3 and 4 of post-wound and p-Smad2/3 was also suppressed. All AO lesions showed accelerated mild wound closure at Day 6, but there were no significant difference. Several papers reported that Smad3 may mediate the signaling pathways that is inhibitory to wound healing, as the deletion of Smad3 leads to enhanced re-epithelialization and contraction of the wound area. This study showed that topical, bio-active ceramic ointment applications accelerated wound closure, re-epithelialization and the suppression of Smad proteins (p-Smad2/3, Smad3). The data revealed that the suppression of Smad3, which was induced by bio-active ceramic resources powder particles affected re-epithelialization and cutaneous wound closure. At the end of this paper, we concluded that bio-active ceramic resources affect cutaneous wound healing by accelerating the re-epithelialization of keratinocytes and that is mediated by the suppression of related protein, Smad3.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea.
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Chung
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Do
authors.forename: Won-Il
authors.initials: WI
authors.lastname: Jeong
authors.forename: Da-Hee
authors.initials: DH
authors.lastname: Jeong
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Park
authors.forename: Mi-Ran
authors.initials: MR
authors.lastname: Ki
authors.forename: Dong-Mi
authors.initials: DM
authors.lastname: Kwak
authors.forename: Soon-Bok
authors.initials: SB
authors.lastname: Kim
authors.forename: Myung-Sook
authors.initials: MS
authors.lastname: Choi
authors.forename: Kyu-Shik
authors.initials: KS
authors.lastname: Jeong
mesh_terms: D000818:Animals; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects; D007150:Immunohistochemistry; D008297:Male; D009824:Ointments / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*; D012867:Skin / Q000187:drug effects*; D051899:Smad2 Protein / Q000378:metabolism; D051900:Smad3 Protein / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D009824:Ointments; D010750:Phosphoproteins; D051899:Smad2 Protein; D051900:Smad3 Protein; D016212:Transforming Growth Factor beta