Characterization of small amounts of peptides and proteins.
The use of microchemical methods for the characterization of both natural and recombinant proteins of biomedical importance is discussed. The methods include gel electrophoresis, high-performance liquid chromatography, protein fragmentation, amino acid analysis and automated Edman degradation. Each procedure is applicable at the picomole level.
Wideman
J
J
Biopolymer Research Department, Roche Research Center, Hoffmann-La Roche Inc., Nutley, NJ 07110, USA.
Pan
Y C
YC
Lahm
H W
HW
Chang
M
M
Stein
S
S