Swapping of periplasmic domains between Brucella suis VirB8 and a pSB102 VirB8 homologue allows heterologous complementation.
A Brucella suis mutant with a nonpolar deletion in the virB8 gene was attenuated in a macrophage infection model. Complementation with the B. suis VirB8 protein expressed from the virB promoter restored virulence. Expression of TraJ, a VirB8 homologue from plasmid pSB102, did not restore virulence; however, virulence was partially restored by a chimeric protein containing the N terminus of the B. suis VirB8 protein and the C-terminal periplasmic domain of TraJ.
Patey
Gilles
G
Institut National de la Santé et de la Recherche Médicale U431, UFR Médecine, CS83021, Avenue Kennedy, 30908 Nîmes Cedex 02, France.
Qi
Zhong
Z
Bourg
Gisele
G
Baron
Christian
C
O'Callaghan
David
D
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D041021:Brucella suis / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*; D002460:Cell Line; D005816:Genetic Complementation Test; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D009154:Mutation; D046928:Peptidoglycan Glycosyltransferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019897:Periplasm / Q000737:chemistry; D010957:Plasmids / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C041427:TraJ protein, bacteria; D046928:Peptidoglycan Glycosyltransferase