Selective solid-phase extraction of cholesterol using molecularly imprinted polymers and its application in different biological samples.
Non-covalent molecularly imprinted polymers (MIPs) of cholesterol were prepared by UV initiated polymerization. A polymer that had the highest binding selectivity and capability was used as solid-phase extraction (SPE) sorbents for direct extraction of cholesterol from different biological samples (human serum, cow milk, yolk, shrimp, pork and beef). The extraction conditions of molecularly imprinted SPE (MISPE) were optimized and the optimum protocol was: conditioning MISPE cartridges with n-hexane, loading with n-hexane, washing with n-hexane and n-hexane:toluene=9:1, respectively, then eluting with chloroform:ethanol:acetic acid=3:1:1. Cholesterol MISPE selectively recognized, effectively trapped and pre-concentrated cholesterol over a concentration range of 10-80 microg/mL. Recoveries ranged from 80.6% to 92.7%, with R.S.D. lower than 9.8%. Under the optimal condition, MISPE recoveries of spiked human serum, yolk, cow milk, shrimp, pork and beef were 91.1%, 80.4%, 86.6%, 78.2%, 81.4% and 80.1%, respectively. Compared with C18 SPE, almost all of the matrix interferences were removed after MISPE, and better baselines and higher selectivity were achieved.
Shi
Yun
Y
MOE Key Laboratory of Environment & Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, PR China.
Zhang
Jiang-Hua
JH
Shi
Dan
D
Jiang
Ming
M
Zhu
Ye-Xiang
YX
Mei
Su-Rong
SR
Zhou
Yi-Kai
YK
Dai
Kang
K
Lu
Bin
B
D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000032:analysis* / Q000097:blood; D004530:Egg Yolk / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D006801:Humans; D008460:Meat / Q000032:analysis; D008892:Milk / Q000737:chemistry; D015394:Molecular Structure; D033561:Penaeidae / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013552:Swine
D011108:Polymers; D002784:Cholesterol