Differential segregation of protective immunity by encoded antigen in DNA vaccine against pseudorabies virus.
A murine model immunized with plasmid DNA vaccine expressing three glycoproteins pCIgB, pCIgC and pCIgD were used to examine the relative potency of major glycoproteins as well as the contribution of immunological parameters in providing protective immunity against the pseudorabies virus (PrV). Among the three glycoprotein-encoded plasmid DNA vaccines, pCIgB produced the strongest response of PrV-specific IgG in the sera. pCIgB and pCIgD also induced a contrast pattern of immunity that was biased to the Th1 and Th2 types, respectively. pCIgC showed the potent inducer of CD8+ T-cell-mediated CTL activity against PrV. In addition, a cocktail vaccination of all three glycoprotein-encoded plasmid DNA vaccines induced the production of both cytokine types, Th1 and Th2 with levels that were the same as that of each immunogen. With regard to protective efficacy, pCIgB induced the most effective protection against a virulent virus challenge and a cocktail vaccination appeared to offer complete protection against a 5 LD50 challenge, but not a 10 LD50 one. pCIgD induced protection that was same as pCIgB, but pCIgC offered no effective protection. These results show the relative potency of the three glycoprotein-encoded PrV DNA vaccines in inducing protective immunity against PrV infection. The results in this study support previous results showing the importance of Th1-type CD4+ T cells and their antibodies in conferring protection.
Yoon
Hyun A
HA
Department of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Jeonju City, Korea.
Aleyas
Abi G
AG
George
Junu A
JA
Park
Seong Ok
SO
Han
Young Woo
YW
Kang
Seong Ho
SH
Cho
Jeong Gon
JG
Eo
Seong Kug
SK
D000818:Animals; D000917:Antibody Formation; D005260:Female; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D022421:Pseudorabies Vaccines / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
D006023:Glycoproteins; D022421:Pseudorabies Vaccines; D019444:Vaccines, DNA