title: Cytological mechanism of pollen abortion resulting from allelic interaction of F1 pollen sterility locus in rice (Oryza sativa L.).
abstract: Pollen abortion is one of the major reasons causing the inter-subspecific F(1) hybrid sterility in rice and is due to allelic interaction of F(1) pollen sterility genes. The microsporogenesis and microgametogenesis of Taichung 65 and its three F(1) hybrids were comparatively studied by using techniques of differential interference contrast microscopy, semi-thin section light microscopy, epifluorescence microscopy and TEM. The results showed that there were differences among the cytological mechanisms of pollen abortion due to allelic interaction at the three F(1) pollen sterility loci. The allelic interaction at S-a locus resulted in microspores unable to extend the protoplasm membrane with the enlargement of the microspore at the middle microspore stage and finally producing empty abortive pollen. The allelic interaction at S-b locus caused asynchronous development of microspores at the middle microspore stage producing stainable abortive pollen. The allelic interaction at S-c locus mainly led to the non-dissolution of the generative cell wall and finally caused the hybrid F(1) mainly producing stainable abortive pollen. Genotypic identification indicated that the abortive pollen were those with S ( j ) allele.
authors.affiliation: Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, 510642, Guangzhou, China.
authors.forename: Zhi-Sheng
authors.initials: ZS
authors.lastname: Zhang
authors.forename: Yong-Gen
authors.initials: YG
authors.lastname: Lu
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Liu
authors.forename: Jiu-Huan
authors.initials: JH
authors.lastname: Feng
authors.forename: Gui-Quan
authors.initials: GQ
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D003433:Crosses, Genetic; D005298:Fertility / Q000235:genetics; D005838:Genotype; D012275:Oryza / Q000235:genetics*; D051479:Plant Infertility / Q000235:genetics*; D018514:Plant Structures / Q000235:genetics; D011058:Pollen / Q000166:cytology* / Q000235:genetics* / Q000648:ultrastructure