Determination of dextromethorphan and its metabolites in plasma by dual column liquid chromatography and fluorimetric detection.
Dextromethorphan and its metabolites were determined in plasma by a dual column liquid chromatographic switching system, after a prepurification on Sep-Pak C(18) cartridges. A polar precolumn (CN) and two hydrophobic analytical columns (C(18)) were used with mobile phases optimised regarding trace enrichment, selectivity and speed by the content of organic modifier. Dextromethorphan and its metabolites could be quantified at 5 ng ml(-1) with an intra-assay precision better than 6% (RSD).
Johansson
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Department of Research and Development, ACO Läkemedel AB, Box 3026, S-171 03 Solna, Sweden.
Svensson
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C