Redistribution of androgen receptors in acquired hormone-refractory prostate cancer cells.
The dynamic translocation of androgen receptors (ARs) in prostate cancer cells after hormone conversion was studied. The prostate cancer cell line LNCaP was converted into androgen-independent sublines after long-term treatment with 5alpha-reductase inhibitor and steroid-depleted medium. Immunohistochemical, immunofluorescent staining and laser scanning microscopy were used to observe the redistribution and serial translocation of ARs in these tumor cells. The androgen-independent tumor cells (LNCaP/Fin and LNCaP/HR) grew slower than native cells with fibroblastic-like characteristics. On immunohistochemical and immunofluorescent double staining, translocation and exocytosis of ARs were noted in androgen- independent tumor cells much more markedly than in native cells. Furthermore, laser-scanning microscopy revealed serial image changes of AR vesicle shifting and exocytosis in androgen-converted tumor cells. Translocation and exocytosis processes were observed in androgen-independent prostate cancer cells. ARs lose partly normal cellular biologic role during hormone manipulation.
Yu
D S
DS
Department of Surgery, Division of Urology, Uro-Oncology Laboratory, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC. yuds@ms21.hinet.net
Hsieh
D S
DS
Chang
S Y
SY
D000970:Antineoplastic Agents / Q000627:therapeutic use; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D021381:Protein Transport; D011944:Receptors, Androgen / Q000378:metabolism*
D000970:Antineoplastic Agents; D011944:Receptors, Androgen