title: Analysis of cell-based RNAi screens.
abstract: RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (http://www.dkfz.de/signaling/cellHTS). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.
authors.affiliation: Signaling and Functional Genomics, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany. m.boutros@dkfz.de
authors.forename: Michael
authors.initials: M
authors.lastname: Boutros
authors.forename: Lígia P
authors.initials: LP
authors.lastname: Brás
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Huber
mesh_terms: D011786:Quality Control; D034622:RNA Interference; D015203:Reproducibility of Results