[Expression of recombinant N-terminal fragment of lacZ gene in Escherichia coli].
Recombinant plasmid pMP1 was used in this investigation. In this plasmid the coding sequence of N-terminal lacZ gene fragment (Mw 43 kD) is cloned under the control of the lamda phage promoter Pr. Depending of recipient strain genotype, inducible or constitutive expression system for recombinant protein can be created on the base of pMP1 plasmid. A comparative analysis of inducible and constitutive overproducing strains has not revealed serious differences in productivity between them, while the use of the constitutive strain at industrial scale can prove more technologically effective.
Kobozev
Iu O
IuO
Pilipenko
V G
VG
D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000235:genetics*
D011994:Recombinant Proteins; D001616:beta-Galactosidase