Flow cytometric determination of the effects of antibacterial agents on Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp. capricolum, and Mycoplasma mycoides subsp. mycoides large colony type.
Flow cytometry together with SYBR green I and propidium iodide was used to study the effects of enrofloxacin, ciprofloxacin, gentamicin, chloramphenicol, oxytetracycline, and tylosin on four mycoplasma species. Inhibition of mycoplasma growth could be detected by as early as 3 h after the start of treatment. The strongest effect was observed with enrofloxacin- and ciprofloxacin-treated cells.
Assunção
Patricia
P
Unidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Arucas, Las Palmas, Spain. passuncao@becarios.ulpgc.es
Antunes
Nuno T
NT
Rosales
Ruben S
RS
Poveda
Carlos
C
Poveda
Jose B
JB
Davey
Hazel M
HM
D000995:Antitubercular Agents / Q000494:pharmacology*; D052160:Benzothiazoles; D004396:Coloring Agents / Q000378:metabolism; D003959:Diamines; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009174:Mycoplasma / Q000187:drug effects* / Q000254:growth & development; D045644:Mycoplasma agalactiae / Q000187:drug effects* / Q000254:growth & development; D045623:Mycoplasma capricolum / Q000187:drug effects* / Q000254:growth & development; D009176:Mycoplasma mycoides / Q000187:drug effects* / Q000254:growth & development; D009930:Organic Chemicals / Q000378:metabolism; D011419:Propidium / Q000378:metabolism; D011804:Quinolines; D015203:Reproducibility of Results
D000995:Antitubercular Agents; D052160:Benzothiazoles; D004396:Coloring Agents; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D011419:Propidium