Biological role of the CXCR4-SDF-1 axis in normal human hematopoietic cells.
Stromal-derived factor (SDF)-1, an alpha-chemokine that binds to G protein-coupled seven transmembrane-spanning receptor, CXCR4, plays an important and unique role in regulating the trafficking of normal hematopoietic stem/progenitor cells and their homing/retention in bone marrow. The same axis also modulates several biological processes in more differentiated cells from the granulocyte-monocytic, erythroid, and megakaryocytic lineages. In this chapter, experimental details are described for the isolation of early human hematopoietic cells, such as CD34+ mononuclear cells, myeloblasts, erythroblasts, and megakaryoblasts. These cells can be used routinely for studying the role of the CXCR4-SDF-1 axis in normal human hematopoiesis.
Majka
Marcin
M
James Graham Brown Cancer Center, University of Louisville, KY, USA.
Ratajczak
Mariusz Z
MZ
D018952:Antigens, CD34 / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000378:metabolism*; D003114:Colony-Forming Units Assay; D016895:Culture Media, Serum-Free; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D019718:Receptors, CXCR4 / Q000378:metabolism*
D018952:Antigens, CD34; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D016895:Culture Media, Serum-Free; D019718:Receptors, CXCR4