Detecting oxygen consumption in the proximity of Saccharomyces cerevisiae cells using self-assembled fluorescent nanosensors.
We describe a strategy for the preparation and self-assembly of fluorescent nanosensors onto Saccharomyces cerevisiae cell surfaces for dynamically measuring oxygen concentration in the proximity of living cells. Amine functionalized polystyrene nanobeads were impregnated with an oxygen-sensitive ruthenium(II) complex and the beads' surface was coated with polyethylenimine. The resulting nanosensors were assembled on individual S. cerevisiae cells in a controlled manner at physiological pH for continuously monitoring oxygen consumption. This approach exemplifies a general scheme for assembling fluorescent nanosensors on cells for the non-invasive, reversible, and real-time measurement of other physiologically relevant processes, such as the efflux of protons and carbon dioxide, or the influx of glucose.
Kuang
Yina
Y
Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, Massachusetts 02155, USA.
Walt
David R
DR
D005456:Fluorescent Dyes; D036103:Nanotechnology / Q000379:methods*; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013499:Surface Properties
D005456:Fluorescent Dyes; D012428:Ruthenium; D010100:Oxygen