Immunostimulating factor isolated from actinobacillus actinomycetemcomitans stimulates monocytes and inflammatory macrophages.
Immunostimulating factor (ISTF) isolated from Actinobacillus actinomycetemcomitans which has been described previously, is distinct from lipopolysaccharide and induces proliferation of B cells. This study was undertaken to investigate whether ISTF might enhance the stimulation of other immune cells. Immunohistochemically, ISTF exhibited a profound stimulating effect on macrophages and dendritic cells as well as B cells in the spleen of BALB/c mice. ISTF was also recognized for its capacity to induce direct activation of mouse macrophages to produce IL-6, TNF-alpha, and NO and MHC class II expression. Therefore, it is postulated that ISTF stimulates macrophages and possibly other cells to produce a wide variety of proinflammatory mediators, which may be involved in the chronicity and tissue destruction of periodontal disease.
Jo
Wol-Soon
WS
Dong-A University Medical Science Research Center (BK21 Program), Busan, Korea.
Yee
Sung-Tae
ST
Yoon
Sik
S
Nam
Byung-Huouk
BH
Do
Eunju
E
Jung
Beom-Seok
BS
Jeong
Soo-Jin
SJ
Hong
Sook-Hee
SH
Yoo
Young-Hyun
YH
Kang
Chi-Dug
CD
Lim
Young-Jin
YJ
Jeong
Min-Ho
MH
Lee
Jae-Dong
JD
D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D004357:Drug Synergism; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D009569:Nitric Oxide / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D009569:Nitric Oxide