title: Diagnosis of human rotavirus in stool specimens: comparison of different methods.
abstract: A total of 345 stool specimens of hospitalized children below 5 years of age with acute gastroenteritis were tested for the presence of rotavirus by Polyacrylamide gel electrophoresis (PAGE), a monoclonal antibody based enzyme immunoassay (EIA) and a latex agglutination test (LAT). Detection rate for PAGE and EIA were 24.9% (345/86) and 20.9% (345/70) respectively. Using PAGE as the standard, the sensitivity and specificity of EIA were 75.6% and 98.1% respectively. The sensitivity of LAT was 70.9% with 100% specificity (LAT was done in only PAGE positive specimens). LAT appeared as the simplest and economic for both bed side and field use.
authors.affiliation: Department of Microbiology, Mymensingh Medical College.
authors.forename: S K
authors.initials: SK
authors.lastname: Paul
authors.forename: S
authors.initials: S
authors.lastname: Tabassum
authors.forename: M N
authors.initials: MN
authors.lastname: Islam
authors.forename: M U
authors.initials: MU
authors.lastname: Ahmed
authors.forename: J U
authors.initials: JU
authors.lastname: Haq
authors.forename: A K
authors.initials: AK
authors.lastname: Shamsuzzaman
mesh_terms: D001459:Bangladesh / Q000453:epidemiology; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D007841:Latex Fixation Tests; D008297:Male; D015995:Prevalence; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000453:epidemiology; D012680:Sensitivity and Specificity