title: cAMP controls oxygen metabolism in mammalian cells.
abstract: The impact of cAMP on ROS-balance in human and mammalian cell cultures was studied. cAMP reduced accumulation of ROS induced by serum-limitation, under conditions in which there was no significant change in the activity of scavenger systems. This effect was associated with cAMP-dependent activation of the NADH-ubiquinone oxidoreductase activity of complex I. In fibroblasts from a patient a genetic defect in the 75 kDa FeS-protein subunit of complex I resulted in inhibition of the activity of the complex and enhanced ROS production, which were reversed by cAMP. A missense genetic defect in the NDUFS4 subunit, putative substrate of PKA, suppressed, on the other hand, the activity of the complex and prevented ROS production.
authors.affiliation: Department of Biomedical Science, University of Foggia, Foggia, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Piccoli
authors.forename: Salvatore
authors.initials: S
authors.lastname: Scacco
authors.forename: Francesco
authors.initials: F
authors.lastname: Bellomo
authors.forename: Anna
authors.initials: A
authors.lastname: Signorile
authors.forename: Arcangela
authors.initials: A
authors.lastname: Iuso
authors.forename: Domenico
authors.initials: D
authors.lastname: Boffoli
authors.forename: Rosella
authors.initials: R
authors.lastname: Scrima
authors.forename: Nazzareno
authors.initials: N
authors.lastname: Capitanio
authors.forename: Sergio
authors.initials: S
authors.lastname: Papa
mesh_terms: D000818:Animals; D002460:Cell Line; D016895:Culture Media, Serum-Free; D000242:Cyclic AMP / Q000502:physiology*; D042967:Electron Transport Complex I / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016895:Culture Media, Serum-Free; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D000242:Cyclic AMP; D013482:Superoxide Dismutase; D042967:Electron Transport Complex I; D005978:Glutathione