title: Detecting transcriptionally active regions using genomic tiling arrays.
abstract: We have developed a method for interpreting genomic tiling array data, implemented as the program TranscriptionDetector. Probed loci expressed above background are identified by combining replicates in a way that makes minimal assumptions about the data. We performed medium-resolution Anopheles gambiae tiling array experiments and found extensive transcription of both coding and non-coding regions. Our method also showed improved detection of transcriptional units when applied to high-density tiling array data for ten human chromosomes.
authors.affiliation: Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, New York, NY 10027, USA. gh74@columbia.edu
authors.forename: Gabor
authors.initials: G
authors.lastname: Halasz
authors.forename: Marinus F
authors.initials: MF
authors.lastname: van Batenburg
authors.forename: Joelle
authors.initials: J
authors.lastname: Perusse
authors.forename: Sujun
authors.initials: S
authors.lastname: Hua
authors.forename: Xiang-Jun
authors.initials: XJ
authors.lastname: Lu
authors.forename: Kevin P
authors.initials: KP
authors.lastname: White
authors.forename: Harmen J
authors.initials: HJ
authors.lastname: Bussemaker
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics; D002877:Chromosomes, Human; D023281:Genomics; D006801:Humans; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic