[Cloning and expression of the pig fast skeletal muscle troponin C gene].
A pig skeletal muscle expressed sequence tag (EST, accession number BM083186) was found to encode amino acid sequences with high homology to human TNNC2. In silico cloning obtained a 843 bp sequence which we named pig fast skeletal troponin C gene (TNNC2). The open reading frame of TNNC2 was between nucleotides 201 - 683, and was confirmed by RT-PCR. The gene encoded a 160 amino acid residue protein and had the highest similarity with human and mouse homologues, with percent identities being 93.6%, 90.5%, respectively. Semi-quantitative RT-PCR analysis of mRNA from different normal tissues indicted that the TNNC2 gene was expressed in the skeletal muscle and the levels of expression were higher in the longissimus dorsi of the Duroc pig than in the same tissue of the Lantang pig.
Tian
Xing-Guo
XG
College of Animal Sciences, South China Agricultural University, Guangzhou 510642, China.
Chen
Yao-Sheng
YS
Ling
Fei
F
Mei
Ying-Jie
YJ
Wang
Chong
C
Luo
Yong-Fa
YF
Li
Jia-Qi
JQ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism; D019209:Troponin C / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D019209:Troponin C