[Identification of six species of sarcosaphagous flies (Diptera) by sequence analysis of cytochrome oxidase subunit I gene (COI) in Weifang].
OBJECTIVE
To identify sarcosaphagous flies and their larvae, pupa.


METHODS
Sarcosaphagous flies and their larvae, pupas were collected from human corpses and their surroundings in the Weifang city. A 304 bp region in COI gene was analyzed by mtDNA sequencing.


RESULTS
The studied region showed no sequence divergence within same species and significant difference were found between different species in all samples.


CONCLUSIONS
It is a practical approach to identify these Sarcosaphagous flies and their larvae, pupas by sequence analysis of the 304bp region of the COI in mtDNA.
Wang
Xin-Jie
XJ
Public Security Bureau of Weifang municipality, Weifang 261061, China.
Wang
Xue-Hai
XH
Diao
Li-Jiang
LJ
Lu
Gui-Ping
GP
D000818:Animals; D001483:Base Sequence; D002681:China; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D004175:Diptera / Q000145:classification / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005554:Forensic Medicine; D017344:Genes, Insect; D006801:Humans; D007814:Larva / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011679:Pupa / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity
D017931:DNA Primers; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV