Methodology of reversible protein labeling for ratiometric fluorescent measurement.
The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique amino acid tag.
Soh
Nobuaki
N
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
Seto
Daisuke
D
Nakano
Koji
K
Imato
Toshihiko
T
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D002021:Buffers; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008954:Models, Biological; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D002021:Buffers; D005456:Fluorescent Dyes; D011506:Proteins