Isolation of meclofenamic acid and two metabolites from equine urine--a comparison between horse and man.
Two metabolites of meclofenamic acid have been isolated from equine urine. Both metabolites are found to be monohydroxylated forms of meclofenamic acid by gas chromatography-mass spectrometry after extractive alkylation. The parent drug and the metabolites are separated by reversed-phase liquid chromatography on a Spherisorb ODS column, using methanol-phosphate buffer eluents and UV detection at 280 nm. The structure of the metabolites is discussed on the basis of LC, TLC and GC-MS data.
Johansson
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IM
Swedish University of Agricultural Sciences, Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Biomedical Center, Box 573, S-751 23 Uppsala, Sweden.
Anlér
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Bondesson
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Schubert
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