title: Blue rayon-anchored technique/Salmonella microsome microsuspension assay as a tool to monitor for genotoxic polycyclic compounds in Santos estuary.
abstract: The most important harbor of Brazil is located in Santos Estuary. In the 1970s, this area was one of the major examples of coastal degradation and although the quality of the environment has improved, the sediment is still contaminated with polycyclic aromatic hydrocarbons (PAHs) and mutagenic activity. Because of sediment dredging and consequently contaminants resuspension, it is useful to have reliable methods to monitor the water quality. Considering that blue rayon (BR) has been successfully used in evaluation of mutagenicity and PAHs content the objective of this work was to verify the applicability and adapt the methodology to monitor the water for mutagenic activity using the BR associated with the Salmonella assay. Analysis of three sites with different levels of contamination was performed using a modification of the BR hanging method denominated in this work BR anchored technique. The microsuspension protocol of the Salmonella/microsome assay was employed with the strain YG1041. The water from the site 1 the most contaminated and under influence of the steel mill discharge presented the highest potency reaching 36,000 revertants/g of BR with S9. Sites 2 and 3 showed less mutagenicity than site 1 with values approximately 1000 revertants/g of BR. We conclude that the BR anchored technique associated with Salmonella assay using YG1041 is a reliable alternative to monitor estuarine waters, especially in regions where sediment resuspension or acute pollution episodes can occur.
authors.affiliation: Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, Av. Prof. Lineu Prestes 580, 05508-900 São Paulo, Brazil. fabiok@usp.br
authors.forename: Fábio
authors.initials: F
authors.lastname: Kummrow
authors.forename: Célia M
authors.initials: CM
authors.lastname: Rech
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Coimbrão
authors.forename: Gisela A
authors.initials: GA
authors.lastname: Umbuzeiro
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000633:toxicity; D001551:Benz(a)Anthracenes / Q000633:toxicity; D001938:Brazil; D002482:Cellulose / Q000031:analogs & derivatives; D004305:Dose-Response Relationship, Drug; D005843:Geography; D007211:Indoles; D008862:Microsomes, Liver / Q000737:chemistry; D009152:Mutagenicity Tests / Q000295:instrumentation / Q000379:methods; D009942:Organometallic Compounds; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D051381:Rats; D045483:Rivers; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D000873:Anthracenes; D001551:Benz(a)Anthracenes; D007211:Indoles; D009942:Organometallic Compounds; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; C079284:blue rayon; C006593:2-anthramine; D002482:Cellulose; C030935:benz(a)anthracene