[A PCR-RFLP analysis to HLA-B gene among Chinese northern Han populations].
OBJECTIVE
To set up the method for analyzing HLA-B gene polymorphism with PCR-RFLP, and to gain population data among northern Chinese Hans of HLA-B's restricted fragments after NlaIII digestion, and to achieve application in forensic medicine practice.


METHODS
Sample DNA was extracted by the phenol/chloroform extraction method, 943 bp-long fragments containing HLA-B exon 2 and 3 were got by PCR. The endonuclease NlaIII was applied to cut the PCR products into polymorphic fragments shorter than 943bp, then PAGE and silver staining were used to detect the digestion results, finally the digestion sites were assured by DNA sequencing.


RESULTS
Along 943bp-long PCR products, 14 length-different fragments, 20 kinds of fragment combinations were got and 6 cutting site were observed after NlaIII digestion.


CONCLUSIONS
HLA-B gene was highly polymorphic among Chinese northern Hans. Even with only one endonuclease, 14 restricted fragments were got and the PIC was great. Such a HLA-B PCR-RFLP analysis will have values in forensic medicine applications.
Zeng
Zhao-Shu
ZS
Department of Forensic Medicine, School of Basic Medicine, Zhengzhou University, Zhengzhou 450052, China.
Ding
Mei
M
Zhu
Yun-Liang
YL
Liu
Zhi-Fang
ZF
Sun
Zhi-Gang
ZG
Huang
Hong-Wu
HW
D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D002681:China / Q000208:ethnology; D004247:DNA / Q000302:isolation & purification; D005091:Exons; D005554:Forensic Medicine / Q000379:methods; D005787:Gene Frequency; D015235:HLA-B Antigens / Q000097:blood / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
D015235:HLA-B Antigens; D004247:DNA