title: An acute and coincident increase in FABP expression and lymphatic lipid and drug transport occurs during intestinal infusion of lipid-based drug formulations to rats.
abstract: OBJECTIVE
  To determine a) whether administration of lipid-based formulations can acutely up-regulate the intestinal expression of I-FABP and L-FABP and b) whether this occurs coincidentally with an increase in intestinal lymphatic lipid and drug transport.
  
  
  METHODS
  The expression of I-FABP and L-FABP mRNA (using q-PCR) and protein (using immunohistochemistry and Western blotting) in enterocytes was compared with data describing transport of lipid and drug into intestinal lymph following infusion of a set of lipid-based formulations.
  
  
  RESULTS
  Administration of relatively small amounts of oleic acid (5-20 mg/h) over a 5 h period to rats acutely up-regulated the expression, and altered the intracellular distribution of, I-FABP and L-FABP in the enterocytes of the small intestinal epithelia. The increase in expression of I-FABP and L-FABP correlated well with previous data describing the transport of lipid and drug into intestinal lymph following infusion of the same formulations.
  
  
  CONCLUSIONS
  The expression and intracellular distribution of I-FABP and L-FABP are acutely influenced by lipid infusion over a time period relevant to feeding or the administration of pharmaceutical lipidic formulations, and these changes occur coincidentally with increased drug transport into the lymphatics.
authors.affiliation: Department of Pharmaceutics, Victorian College of Pharmacy, Monash University, 381 Royal Parade, Parkville, Victoria 3052, Australia.
authors.forename: Natalie L
authors.initials: NL
authors.lastname: Trevaskis
authors.forename: Chun Min
authors.initials: CM
authors.lastname: Lo
authors.forename: Li Yun
authors.initials: LY
authors.lastname: Ma
authors.forename: Patrick
authors.initials: P
authors.lastname: Tso
authors.forename: Helen R
authors.initials: HR
authors.lastname: Irving
authors.forename: Christopher J H
authors.initials: CJ
authors.lastname: Porter
authors.forename: William N
authors.initials: WN
authors.lastname: Charman
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D015153:Blotting, Western; D002626:Chemistry, Pharmaceutical; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007441:Intubation, Gastrointestinal; D008055:Lipids / Q000737:chemistry; D008196:Lymph / Q000502:physiology*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D050556:Fatty Acid-Binding Proteins; D008055:Lipids; D004364:Pharmaceutical Preparations; D012333:RNA, Messenger