title: The mRNA export factor Sus1 is involved in Spt/Ada/Gcn5 acetyltransferase-mediated H2B deubiquitinylation through its interaction with Ubp8 and Sgf11.
abstract: Sus1 acts in nuclear mRNA export via its association with the nuclear pore-associated Sac3-Thp1-Cdc31 complex. In addition, Sus1 plays a role in transcription through its interaction with the Spt/Ada/Gcn5 acetyltransferase (SAGA) complex. Here, we have analyzed function and interaction of Sus1 within the SAGA complex. We demonstrate that Sus1 is involved in the SAGA-dependent histone H2B deubiquitinylation and maintenance of normal H3 methylation levels. By deletion analyses, we show that binding of Sus1 to SAGA depends on the deubiquitinylating enzyme Ubp8 and Sgf11. Moreover, a stable subcomplex between Sus1, Sgf11, and Ubp8 could be dissociated from SAGA under high salt conditions. In vivo recruitment of Sus1 to the activated GAL1 promoter depends on Ubp8 and vice versa. In addition, histones coenrich during SAGA purification in a Sus1-Sgf11-Ubp8-dependent way. Interestingly, sgf11 deletion enhances the mRNA export defect observed in sus1delta cells. Thus, the Sus1-Sgf11-Ubp8 module could work at the junction between SAGA-dependent transcription and nuclear mRNA export.
authors.affiliation: Biochemie-Zentrum der Universität Heidelberg, INF328, D-69120, Heidelberg, Germany.
authors.forename: Alwin
authors.initials: A
authors.lastname: Köhler
authors.forename: Pau
authors.initials: P
authors.lastname: Pascual-García
authors.forename: Ana
authors.initials: A
authors.lastname: Llopis
authors.forename: Meritxell
authors.initials: M
authors.lastname: Zapater
authors.forename: Francesc
authors.initials: F
authors.lastname: Posas
authors.forename: Ed
authors.initials: E
authors.lastname: Hurt
authors.forename: Susana
authors.initials: S
authors.lastname: Rodríguez-Navarro
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism; D021581:Active Transport, Cell Nucleus; D010450:Endopeptidases / Q000378:metabolism; D005696:Galactosidases / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D006657:Histones / Q000378:metabolism*; D008745:Methylation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012492:Salts / Q000737:chemistry; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D006657:Histones; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C507785:SAGA complex, S cerevisiae; C489245:SGF11 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012492:Salts; C481300:Sus1 protein, S cerevisiae; D015534:Trans-Activators; D014157:Transcription Factors; D025801:Ubiquitin; D000123:Acetyltransferases; D005696:Galactosidases; D010450:Endopeptidases; C503322:UBP8 protein, S cerevisiae