title: The biofilm matrix of Pseudomonas sp. OX1 grown on phenol is mainly constituted by alginate oligosaccharides.
abstract: The structure of the major constituent of the biofilm matrix produced by Pseudomonas sp. OX1, when grown on phenol as the sole carbon source is described. This investigation, carried out by chemical analysis, NMR spectroscopy and MALDI-TOF MS spectrometry, showed the presence of an oligosaccharide blend with the typical alginate structure, namely (1-->4) substituted beta-D-mannuronic (ManA) and alpha-L-guluronic acid (GulA). GulA residues were non-acetylated whereas ManA was always O-acetylated at C-2 or C-3.
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Via Cintia, 4 I-80126 Napoli, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Leone
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
authors.forename: Fabiana
authors.initials: F
authors.lastname: Alfieri
authors.forename: Valeria
authors.initials: V
authors.lastname: Cafaro
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Alberto
authors.initials: A
authors.lastname: Di Donato
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
mesh_terms: D000464:Alginates / Q000737:chemistry*; D018441:Biofilms; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry*; D019800:Phenol; D011135:Polysaccharides, Bacterial; D011549:Pseudomonas / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D019800:Phenol; D020723:Glucuronic Acid