title: CpG oligodeoxynucleotides induce Ca2+-dependent phospholipase D activity leading to phagolysosome maturation and intracellular mycobacterial growth inhibition in monocytes.
abstract: Synthetic oligodeoxynucleotides containing CpG motifs (CpG ODN) have been reported to induce antimycobacterial activity both in vitro and in vivo. The present study analyzes the signals leading to CpG ODN-induced antimicrobial activity in monocytes. In this context, CpG, but not GpC, ODN induced cytosolic Ca2+ influx of extracellular origin which, in turn, activated host phospholipase D (PLD). The production of CpG-induced PLD-dependent phosphatidic acid induced the maturation of phagolysosomes and intracellular mycobacterial growth inhibition. These results show the presence of an antimicrobial pathway in monocytes, mediated by Ca2+-dependent PLD which can be useful for the exploitation of novel anti-tuberculosis immunotherapy approaches.
authors.affiliation: Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Greco
authors.forename: Marco
authors.initials: M
authors.lastname: De Spirito
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Papi
authors.forename: Marco
authors.initials: M
authors.lastname: Fossati
authors.forename: Giovanni
authors.initials: G
authors.lastname: Auricchio
authors.forename: Maurizio
authors.initials: M
authors.lastname: Fraziano
mesh_terms: D002118:Calcium / Q000502:physiology; D045744:Cell Line, Tumor; D018899:CpG Islands / Q000502:physiology; D004789:Enzyme Activation; D006801:Humans; D009000:Monocytes / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D010588:Phagosomes / Q000502:physiology*; D010739:Phospholipase D / Q000502:physiology*
chemical_list: C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D010739:Phospholipase D; D002118:Calcium