YidC-mediated membrane insertion of assembly mutants of subunit c of the F1F0 ATPase.
YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli. We have recently demonstrated that proteoliposomes containing only YidC support both the stable membrane insertion and the oligomerization of the c subunit of the F(1)F(0) ATP synthase (F(0)c). Here we have shown that two mutants of F(0)c unable to form a functional F(1)F(0) ATPase interact with YidC, require YidC for membrane insertion, but fail to oligomerize. These data show that oligomerization is not essential for the stable YidC-dependent membrane insertion of F(0)c consistent with a function of YidC as a membrane protein insertase.
Kol
Stefan
S
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands.
Turrell
Bryony R
BR
de Keyzer
Jeanine
J
van der Laan
Martin
M
Nouwen
Nico
N
Driessen
Arnold J M
AJ
D019943:Amino Acid Substitution / Q000235:genetics*; D025243:Bacterial Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D007425:Intracellular Membranes / Q000201:enzymology*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000502:physiology*; D011499:Protein Processing, Post-Translational / Q000235:genetics; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D021122:Protein Subunits; C412443:YIDC protein, E coli; C074429:atpE protein, E coli; D025243:Bacterial Proton-Translocating ATPases