Antenatal Ureaplasma urealyticum respiratory tract infection stimulates proinflammatory, profibrotic responses in the preterm baboon lung.
Chronic inflammation and fibrosis are hallmarks of lung pathology of newborn Ureaplasma infection. We hypothesized that antenatally acquired Ureaplasma stimulates a chronic inflammatory, profibrotic immune response that contributes to lung injury, altered developmental signaling, and fibrosis. Lung specimens from 125-d gestation baboon newborns ventilated for 14 d that were either infected antenatally with Ureaplasma serovar 1 or noninfected, and 125-d and 140-d gestational controls were obtained from the Baboon BPD Resource Center (San Antonio, TX). Trichrome stain to assess fibrosis and immunohistochemistry for alpha-smooth muscle actin (alpha-SMA) and transforming growth factor beta1 (TGFbeta1) were performed. Lung homogenates were analyzed by enzyme-linked immunosorbent assay (ELISA) for cytokines [tumor necrosis factor alpha (TNFalpha), interleukin (IL)-1beta, TGFbeta1, oncostatin M (OSM), IL-10, and interferon gamma (IFNgamma)] and the chemokine MCP-1 and by Western blot for Smad2, Smad3, and Smad7. Compared with noninfected ventilated and gestational controls, Ureaplasma-infected lungs demonstrated more extensive fibrosis, increased alpha-SMA and TGFbeta1 immunostaining, and higher concentrations of active TGFbeta1, IL-1beta, and OSM, but no difference in IL-10 levels. There was a trend toward higher Smad2/Smad7 and Smad3/Smad7 ratios in Ureaplasma lung homogenates, consistent with up-regulation of TGFbeta1 signaling. Collectively, these data suggest that a prolonged proinflammatory response initiated by intrauterine Ureaplasma infection contributes to early fibrosis and altered developmental signaling in the immature lung.
Viscardi
Rose M
RM
Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD 21201, USA. rviscard@umaryland.edu
Atamas
Sergei P
SP
Luzina
Irina G
IG
Hasday
Jeffrey D
JD
He
Ju-Ren
JR
Sime
Patricia J
PJ
Coalson
Jacqueline J
JJ
Yoder
Bradley A
BA
D000199:Actins / Q000032:analysis; D000818:Animals; D000831:Animals, Newborn; D018932:Chemokine CCL2 / Q000032:analysis / Q000378:metabolism; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000032:analysis / Q000378:metabolism*; D008168:Lung / Q000737:chemistry / Q000382:microbiology / Q000473:pathology; D009130:Muscle, Smooth; D010215:Papio; D047928:Premature Birth / Q000382:microbiology* / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D012121:Respiration, Artificial; D012141:Respiratory Tract Infections / Q000378:metabolism / Q000473:pathology; D051785:Smad Proteins / Q000032:analysis / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000378:metabolism; D016869:Ureaplasma Infections / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D016990:Ureaplasma urealyticum
D000199:Actins; D018932:Chemokine CCL2; D016207:Cytokines; D018836:Inflammation Mediators; D051785:Smad Proteins; D016212:Transforming Growth Factor beta