[An initial investigation on the in vitro culture system of primordial germ cells in golden hamsters].
To establish the in vitro culture system of primordial germ cells (PGCs) of golden hamsters, PGCs of hamster were isolated from genital ridge of embryos at 10. 5th dpc (day post coitum), obtained by enzyme-mechanical method, and cultured on feeder cells. Then the PGCs were identified by alkaline phosphatase (ALP) activity staining. In order to induce the PGCs to differentiate in vitro, we removed the differential inhibition factors in the conditioned medium and observed the formation of embryoids and differentiated cells from three layers. The result showed that the pluripotent primordial germ cells could be successfully obtained from the golden hamsters at 10. 5th dpc by the enzyme-mechanical method and that PGCs were identified by both their strong positive reaction in ALP activity staining test and their differentiation into three-layer derived cells in vitro. The result suggests that the establishment of in vitro PGCs culture system of golden hamsters will provide new cell source for biomedical engineering.
Li
Hong
H
Deparment of Environmental Health, Sichuan University, Chengdu 610041, China.
Zhang
Hao
H
Liang
Ying
Y
Zhan
Li
L
Wu
Desheng
D
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D006224:Cricetinae; D004622:Embryo, Mammalian / Q000166:cytology*; D005854:Germ Cells / Q000166:cytology*; D008647:Mesocricetus