title: New derivatization method using ferrocene reagents for the determination of steroid glucuronides by high-performance liquid chromatography with electrochemical detection.
abstract: New derivatization methods using ferrocene reagents have been developed for the determination of steroid glucuronides by high-performance liquid chromatography with electrochemical detection. Condensation of glucuronides with 2-ferrocenylethylamine is effected in the presence of water-soluble carbodiimide and 1-hydroxybenzotriazole. The resulting amides show the satisfactory sensitivity at +0.45 V versus Ag/AgCl reference electrode, with a detection limit of 0.5 pmole. The present method is found to be applicable to the estimation of estrogen glucuronyltransferase activities and estrogen glucuronides in pregnancy urine.
authors.affiliation: Pharmaceutical Institute, Tohoku University, Aobayama, Sendai 980, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: E
authors.initials: E
authors.lastname: Nagashima
authors.forename: S
authors.initials: S
authors.lastname: Orii
authors.forename: T
authors.initials: T
authors.lastname: Nambara