title: [The relationship between histone acetylation modification and IFN-gamma responsive gene regulation].
abstract: OBJECTIVE
  To study the role of histone modification in the regulation of IFN-gamma-activated gene using chromatin immunoprecipitation technique.
  
  
  METHODS
  Real-time PCR was used to measure the mRNA level of interferon-gamma-inducible protein 10 (IP-10) in Hela cells. Chromatin immunoprecipitation combined with Real-time PCR was used to check the histone H4 acetylation level at IFN-stimulated response element (ISRE) locus of IP-10 gene.
  
  
  RESULTS
  IP-10 was strongly activated by IFN-gamma. The histone H4 deacetylation happened at the ISRE locus when IP-10 was induced by IFN-gamma. The activation of IP-10 and the deacetylation of histone H4 at the ISRE site induced by IFN-gamma were inhibited or blocked by histone deacetylase (HDAC) inhibitor trichostatin A (TSA).
  
  
  CONCLUSIONS
  The histone H4 deacetylation at the ISRE site is related with the activation of IP-10 by IFN-gamma.
authors.affiliation: Key Laboratory of Molecular Biology of Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
authors.forename: Jin-jun
authors.initials: JJ
authors.lastname: Guo
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Ai-long
authors.initials: AL
authors.lastname: Huang
mesh_terms: D000107:Acetylation; D054357:Chemokine CXCL10 / Q000378:metabolism*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
chemical_list: C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D006657:Histones; D012333:RNA, Messenger; D007371:Interferon-gamma; D006655:Histone Deacetylases