title: Determination of cortisol and cortisone in urine using high-performance liquid chromatography with UV detection.
abstract: The application of reversed-phase gradient high-performance liquid chromatography with UV detection to the determination of cortisol and cortisone in 24-h urine samples is described. The method employs Sep-pak C18 cartridges for the part-purification and concentration of the corticosteroids, with sample enrichment at the head of an HPLC pre-column and separation using water/acetonitrile gradient. The internal standard is 6alpha-methylprednisolone. Measurement of both cortisone and cortisol provides further information on adrenocortical function. 24-hour excretion rate data from normal subjects are reported.
authors.affiliation: University of Oxford, Department of Biological Anthropology, 58 Banbury Road, Oxford OX2 6QS, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: Jenner
authors.forename: J
authors.initials: J
authors.lastname: Richards