Isolation and identification of SA and JA inducible protein kinase gene OsSJMK1 in rice.
On the basis of a JA-inducible EST, the full length cDNA of a putative protein kinase gene, OsSJMKJ, was isolated from rice (Oryza sativa L.). The predicted protein of this gene, consisting of 498 amino acids with a predicted PI value of 8.43, contains a mitogen-activated protein kinase (MAPK) domain with a dual-phosphorylation activation motif TDY (Thr-Asp-Tyr) and a C-terminus domain that feature a typical MAPK belonging to subgroup V. OsSJMK1 showed 81% sequence identity to the reported BWMK1, a blast fungus and wounding inducible protein, in the N-terminus kinase domain but had low sequence identity in the C-terminus domain. Despite the high similarity of gene structure between OsSJMK1 and BWMK1, the expression of OsSJMK1 was weakly induced by blast fungus but not wounding, rather it was transiently induced by jasmonic acid (JA) and salicylic acid (SA) at early stages of treatment. This gene was not induced by other hormones (such as ABA) or abiotic stresses, such as drought and salinity. The low level of transcript of this gene was detected in the various tissues of rice. The results suggest that OsSJMK1 might be activated specifically by JA and SA and involved in defense signaling pathways.
Ning
Jing
J
National Key Laboratory of Crop Genetic Improvement, National Plant Gene Research Center (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
Yuan
Bin
B
Xie
Ka-Bin
KB
Hu
Hong-Hong
HH
Wu
Cong-Qing
CQ
Xiong
Li-Zhong
LZ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology*; D018076:DNA, Complementary / Q000032:analysis; D004789:Enzyme Activation / Q000187:drug effects; D018506:Gene Expression Regulation, Plant; D020928:Mitogen-Activated Protein Kinases / Q000145:classification / Q000378:metabolism*; D008969:Molecular Sequence Data; D052247:Nitric Oxide Synthase Type II; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology*; D011494:Protein Kinases / Q000187:drug effects / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000502:physiology*
D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; C011006:jasmonic acid; D052247:Nitric Oxide Synthase Type II; D011494:Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D020156:Salicylic Acid