Similarities and differences in interactions of the activity-enhancing chemoreceptor pentapeptide with the two enzymes of adaptational modification.
Sensory adaptation and chemotaxis by Escherichia coli require a specific pentapeptide at the chemoreceptor carboxyl terminus. This sequence binds the two enzymes of receptor adaptational modification, enhancing catalysis, but with different binding features and mechanisms. We investigated the relative importance of each pentapeptide side chain for the two enhancing interactions.
Lai
Wing-Cheung
WC
Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA.
Barnakova
Ludmila A
LA
Barnakov
Alexander N
AN
Hazelbauer
Gerald L
GL
D001665:Binding Sites; D002633:Chemotaxis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000032:analysis / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000032:analysis / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D015398:Signal Transduction
D029968:Escherichia coli Proteins; D009842:Oligopeptides; D010455:Peptides; D008780:Methyltransferases