Simple enzymatic in situ generation of dihydroxyacetone phosphate and its use in a cascade reaction for the production of carbohydrates: increased efficiency by phosphate cycling.
A new enzymatic method for the generation of dihydroxyacetone phosphate (DHAP) using the acid phosphatase from Shigella flexneri (PhoN-Sf) and the cheap phosphate donor pyrophosphate (PPi) is described. The utility of this method was demonstrated in an aldolase-catalyzed condensation carried out in one pot in which DHAP was generated and coupled to propionaldehyde to give a yield of 53% of the isolated dephosphorylated end product.
van Herk
Teunie
T
Van't Hoff Institute for Molecular Sciences, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS Amsterdam, The Netherlands.
Hartog
Aloysius F
AF
Schoemaker
Hans E
HE
Wever
Ron
R
D000447:Aldehydes / Q000737:chemistry; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000138:chemical synthesis* / Q000737:chemistry; D004099:Dihydroxyacetone Phosphate / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010710:Phosphates / Q000737:chemistry*; D012763:Shigella flexneri / Q000201:enzymology
D000447:Aldehydes; D002241:Carbohydrates; D010710:Phosphates; D004099:Dihydroxyacetone Phosphate; C116609:3-hydroxybutanal; C005556:propionaldehyde