Metalloproteinase-9 in circulating monocytes in pulmonary hypertension.
The role of matrix metalloproteinases (MMPs) in pulmonary hypertension (PH) is complex as MMPs are involved in both the vascular and cardiac remodelling associated with PH. To gain insight into this problem, monocytes were isolated from pulmonary arterial blood in patients suffering from PH, related to chronic obstructive pulmonary disease (n = 6), chronic pulmonary thromboembolism (n = 3) or pulmonary arterial hypertension (n = 8). The severity of PH was associated with decreases in cardiac index (CI) and mixed venous blood oxygen saturation (SO(2)), and an increase in right atrial pressure (). Monocyte pro-MMP-9 content (zymography) was positively correlated with SO(2) (r = 0.73, P < 0.05) and CI (r = 0.66, P < 0.05), and negatively with (r = 0.54, P < 0.05); there was no significant correlation with pulmonary vascular resistance. In conclusion, the pro-MMP-9 content of circulating monocytes was lower in the more severe forms of PH which showed heart failure suggesting that such MMP enzymatic activity reflects heart failure following pulmonary vascular and myocardial remodelling in PH.
Cantini-Salignac
Caroline
C
Pharmacology Laboratory, Faculté de Pharmacie, Université Henri Poincaré, Nancy, France.
Lartaud
Isabelle
I
Schrijen
Francine
F
Atkinson
Jeffrey
J
Chabot
François
F
D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure; D002303:Cardiac Output, Low / Q000097:blood / Q000209:etiology / Q000503:physiopathology; D005260:Female; D006325:Heart Atria / Q000503:physiopathology; D006801:Humans; D006976:Hypertension, Pulmonary / Q000097:blood* / Q000201:enzymology / Q000209:etiology / Q000503:physiopathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology*; D010100:Oxygen / Q000097:blood; D011651:Pulmonary Artery / Q000503:physiopathology; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000150:complications / Q000503:physiopathology; D011655:Pulmonary Embolism / Q000097:blood / Q000150:complications / Q000503:physiopathology; D012129:Respiratory Function Tests; D012720:Severity of Illness Index; D014655:Vascular Resistance; D020257:Ventricular Remodeling
D015415:Biomarkers; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D010100:Oxygen