title: Characterization, isolation, and differentiation of murine skin cells expressing hematopoietic stem cell markers.
abstract: As the phenotype of adult dermal stem cells is still elusive, and the hematopoietic stem cell is one of the best-characterized stem cells in the body, we tested dermal cell suspensions, sections, and wholemounts in newborn and adult mice for hematopoietic stem cell marker expression. Phenotypic analysis revealed that a small population of CD45(+) cells and a large population of CD45(-) cells expressed CD34, CD117, and stem cell antigen-1 molecules. When cultivated in selected media supplemented with hematopoietic cytokines, total dermal cells, lineage(-), and/or highly enriched phenotypically defined cell subsets produced hematopoietic and nonhematopoietic colonies. When injected into lethally irradiated recipient mice, a small percentage of newborn dermal cells was able to migrate into hematopoietic tissues and the skin and survived through the 11-month monitoring period. Our ability to isolate a candidate autologous stem cell pool will make these cells ideal vehicles for genetic manipulation and gene therapy.
authors.affiliation: Division of Dermatology, Vienna Competence Center, Medical University of Vienna, Vienna 1090, Austria.
authors.forename: Simone
authors.initials: S
authors.lastname: Meindl
authors.forename: Uwe
authors.initials: U
authors.lastname: Schmidt
authors.forename: Christine
authors.initials: C
authors.lastname: Vaculik
authors.forename: Adelheid
authors.initials: A
authors.lastname: Elbe-Bürger
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis*; D000950:Antigens, Ly / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis*; D012867:Skin / Q000166:cytology / Q000276:immunology*
chemical_list: D018952:Antigens, CD34; D000950:Antigens, Ly; D019009:Proto-Oncogene Proteins c-kit; D017493:Leukocyte Common Antigens