Effects of crocetin on the matrix metalloproteinases in cardiac hypertrophy induced by norepinephrine in rats.
The effects of crocetin on the cardiac hypertrophy induced by long-term treatment with norepinephrine (NE) in rats have been investigated. The activities of matrix metalloproteinases (MMP-2 and MMP-9) have been assayed by gelatin SDS-PAGE zymography. The expressions of MMP-2 and MMP-9 were detected by RT-PCR. ATPase activity and hydroxyproline contents were measured with a commercial kit. The results show that crocetin blocked the development of left ventricular hypertrophy induced by NE, decreased the level of collagen in myocardium, enhanced both the Na+-K+ ATPase activity in cardiac tissue and the Ca2+-Mg2+ ATPase activity in mitochondria and inhibited significantly the activity of MMP-2 and the expressions of MMP-2 and MMP-9. These results suggest that crocetin may prevent cardiac hypertrophy induced by NE in rats.
Shen
X-C
XC
Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China.
Y-Lu
Qian
Z-Y
ZY
D000818:Animals; D006332:Cardiomegaly / Q000139:chemically induced / Q000201:enzymology* / Q000517:prevention & control*; D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D003094:Collagen / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D015394:Molecular Structure; D009638:Norepinephrine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014801:Vitamin A / Q000031:analogs & derivatives
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; C487773:trans-sodium crocetinate; D014801:Vitamin A; D002338:Carotenoids; D003094:Collagen; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D009638:Norepinephrine