[Therapeutic effect of glucose-6-phosphate polyclonal antibody on vasogenic brain edema in rats].
OBJECTIVE
To observe the therapeutic effect of glucose-6-phosphate polyclonal antibody (G-6-P pAb) on vasogenic brain edema (VBE) in rats.


METHODS
Sixty Wistar rats were randomly divided into normal control group, VBE group, mannitol-treated edema group, and G-6-P pAb-treated edema group. After establishment of rat models of VBE by intraperitoneal injection of phenylephrine in the latter 3 groups, mannitol was injected through the femoral vein in mannitol group and G-6-P pAb injected intraperitoneally in G-6-P pAb group. The permeability of the blood-brain barrier (BBB) was determined by Evans blue (EB) extravasation method, and the brain water content in the gray and white matter measured with a moisture analyzer.


RESULTS
G-6-P pAb administration significantly reduced the permeability of BBB as well as the water content in the white matter in comparison with mannitol treatment (P<0.01), but the two treatments showed no obvious difference in reducing the water content in the gray matter (P>0.05).


CONCLUSIONS
Changes in G-6-P activity results in BBB permeability alteration in the condition of VBE, and G-6-P pAb has a selective therapeutic effect against VBE, especially white matter edema.
Tao
Zhen
Z
Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. tzhappy1314@126.com
Lu
Bing-xun
BX
Zhang
Yun-zhou
YZ
Cheng
Gang
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use*; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000503:physiopathology; D001929:Brain Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D002199:Capillary Permeability / Q000187:drug effects; D005260:Female; D019298:Glucose-6-Phosphate / Q000276:immunology*; D008297:Male; D010656:Phenylephrine; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D010656:Phenylephrine; D019298:Glucose-6-Phosphate