Protein analysis with terbium (III) and sodium dodecyl sulphonate by a second-order scattering technique.
This is the first report of terbium(III) as a probe of second-order scattering (SOS) for the determination of proteins in human serum at nanogram levels. A sensitive method has been developed using light scattering, based on the fact that the weak SOS of proteins can be enhanced in the presence of terbium(III) and sodium dodecyl sulphonate (SDS). With this method, 7.0 x 10(-9)-1.0 x 10(-5) g/mL human serum albumin (HSA) and 5.0 x 10(-9)-5.0 x 10(-6) g/mL gamma-globulin can be determined; the detection limits were 4.4 ng/mL for HSA and 3.1 ng/mL for gamma-globulin. The method has been applied to the detection of total proteins in human serum samples, and the results are consistent with those obtained by the Coomassie brilliant blue (CBB) G-250 assay.
Li
Aiyun
A
Department of Chemistry, Beijing Normal University, Beijing 100875, People's Republic of China.
Zhao
Huichun
H
Jin
Linpei
L
D001798:Blood Proteins / Q000032:analysis*; D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D036103:Nanotechnology / Q000379:methods; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry; D013725:Terbium / Q000737:chemistry*; D005719:gamma-Globulins / Q000032:analysis
D001798:Blood Proteins; D012709:Serum Albumin; D013501:Surface-Active Agents; D005719:gamma-Globulins; D013725:Terbium; D012967:Sodium Dodecyl Sulfate