title: Control of acetyl-coenzyme A synthetase (AcsA) activity by acetylation/deacetylation without NAD(+) involvement in Bacillus subtilis.
abstract: Posttranslational modification is an efficient mechanism for controlling the activity of structural proteins, gene expression regulators, and enzymes in response to rapidly changing physiological conditions. Here we report in vitro and in vivo evidence that the acuABC operon of the gram-positive soil bacterium Bacillus subtilis encodes a protein acetyltransferase (AcuA) and a protein deacetylase (AcuC), which may control the activity of acetyl-coenzyme A (CoA) synthetase (AMP-forming, AcsA) in this bacterium. Results from in vitro experiments using purified proteins show that AcsA is a substrate for the acetyl-CoA-dependent AcuA acetyltransferase. Mass spectrometry analysis of a tryptic digest of acetylated AcsA (AcsA(Ac)) identified residue Lys549 as the sole modification site in the protein. Unlike sirtuins, the AcuC protein did not require NAD(+) as cosubstrate to deacetylate AcsA(Ac). The function of the putative AcuB protein remains unknown.
authors.affiliation: Department of Bacteriology, 144A Enzyme Institute, 1710 University Avenue, Madison, WI 53726-4087, USA.
authors.forename: Jeffrey G
authors.initials: JG
authors.lastname: Gardner
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Grundy
authors.forename: Tina M
authors.initials: TM
authors.lastname: Henkin
authors.forename: Jorge C
authors.initials: JC
authors.lastname: Escalante-Semerena
mesh_terms: D000106:Acetate-CoA Ligase / Q000235:genetics / Q000378:metabolism*; D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000378:metabolism; D009243:NAD; D009876:Operon
chemical_list: C089829:AcuC protein, Bacillus subtilis; D001426:Bacterial Proteins; D009243:NAD; D000123:Acetyltransferases; C089827:acuA protein, Bacillus subtilis; D000106:Acetate-CoA Ligase