[An involvement of polokinases in control of progress of the cell-cycle--the mechanism of transient translocation and formation of an activated protein-protein complexes during mitosis].
Polokinases are a subfamily of the mitotic serine/threonine kinases involved in coordination of a run of mitosis of eukaryotic cells. The main polo-like-kinase 1p (PLK1) is a passenger protein transiently localized to centrosomes, kinetochores and central spindle during mitosis and is required for bi-orientation of the normal metaphase spindle. Its activity is regulated at the level of protein stability and by action of upstream kinases, so that it peaks in metaphase and drops as cells exit mitosis. Regulation of location and activity of Plk1p is bi-phasic: the COOH terminal polo box domain binds to an array of mitotic phosphoproteins and followed by an allosteric conformation is activated to phosphorylate many its substrates. These mode of action involves polokinases into critical transitions of the cell cycle phases, and in control at some checkpoints of this cycle.
Kaczanowska
Janina
J
Instytut Zoologii, Wydział Biologii, Uniwersytet Warszawski, Warszawa. kaczan@biol.uw.edu.pl
Piwońska
Dominika
D
Kaczanowski
Andrzej
A
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D018385:Centrosome / Q000201:enzymology; D048749:Cytokinesis; D029721:Drosophila Proteins; D016195:G2 Phase; D006801:Humans; D007700:Kinetics; D008938:Mitosis / Q000502:physiology*; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011494:Protein Kinases / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D015398:Signal Transduction; D008941:Spindle Apparatus / Q000201:enzymology / Q000502:physiology; D000097869:Polo-Like Kinase 1
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D046912:Multiprotein Complexes; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1