Inhibition of potassium- and ischemia-evoked [3H] D-aspartate release from isolated bovine retina by cannabinoids.
We investigated the effect of cannabinoids on potassium chloride (K+)- and ischemia-induced [3H]D-aspartate release from isolated bovine retinae. The superfusion method was employed for studies of [3H]-neurotransmitter release. Cannabinoid receptor CB1 agonists, but not the CB2 agonist JWH 015, inhibited K+ -induced [3H]D-aspartate release from bovine retinae with the following rank order of activity: anandamide > ACEA > methanandamide > WIN 55,212-2. In the ischemic model, the rank order of activity was as follows: methanandamide > ACEA > WIN 55,212-2. The CB1 receptor antagonist AM 251 blocked inhibitory responses produced by cannabinoids in both experimental conditions. In conclusion, cannabinoids inhibit evoked [3H]D-aspartate release from isolated bovine retinae via an effect on CB1 receptors.
Opere
Catherine A
CA
Department of Pharmacy Sciences, School of Pharmacy and Health Professions, Creighton University Medical Center, Omaha, Nebrasaka 68178, USA. copere@creighton.edu
Zheng
Wei Dong
WD
Zhao
Min
M
Lee
Jin Sook
JS
Kulkarni
Kaustubh H
KH
Ohia
Sunny E
SE
D000818:Animals; D002186:Cannabinoids / Q000494:pharmacology*; D002417:Cattle; D026603:D-Aspartic Acid / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004195:Disease Models, Animal; D066298:In Vitro Techniques; D007511:Ischemia / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D011189:Potassium Chloride / Q000494:pharmacology*; D012160:Retina / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
D002186:Cannabinoids; D026603:D-Aspartic Acid; D011189:Potassium Chloride