Comparative immunomodulatory effect of scopoletin on tumoral and normal lymphocytes.
Some coumarins possess enhancing effects on lymphocyte mitogen responsiveness. In this investigation, the activity of scopoletin, a coumarin that has been isolated from different plants and in this case specifically from T. cordata Mill., was evaluated. For this purpose, normal T lymphocytes and a hyperproliferative T lymphoma cell line were used. Scopoletin was found to exert a dual action on tumoral lymphocytes exhibiting both a cytostatic and a cytotoxic effect. These effects varied with the concentrations analysed and the time of cell incubation (EC(50): 251+/-15 microg/ml) and were associated to the induction of apoptosis. Scopoletin induced cell proliferation on normal T lymphocytes (Proliferation stimulation index: 1 microg/ml scopoletin: 1.26+/-0.1; 10 microg/ml scopoletin: 3+/-0.25; 100 microg/ml scopoletin: 1.86+/-0.08); this stimulatory action was found to be due to the interaction with kinase C (PKC) protein. These results indicate that scopoletin could be a potential antitumoral compound to be used for cancer treatment.
Manuele
María Gabriela
MG
IQUIMEFA (UBA-CONICET), Junin 956 2 do piso CP: 1113, Buenos Aires, Argentina.
Ferraro
Graciela
G
Barreiro Arcos
Maria Laura
ML
López
Paula
P
Cremaschi
Graciela
G
Anesini
Claudia
C
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D035264:Flowers / Q000737:chemistry; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012603:Scopoletin / Q000302:isolation & purification / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D032384:Tilia / Q000737:chemistry
D007155:Immunologic Factors; D003208:Concanavalin A; D012603:Scopoletin