A role for lysozyme in melanization of Sephadex beads in Anopheles gambiae.
Melanization of foreign targets in the mosquito, Anopheles gambiae, was studied using a model Sephadex bead system. A mosquito factor that was deposited on beads and prevented bead melanization (MPF) was purified. The N-terminal sequence of the factor identified it as lysozyme c-1 (Lys c-1). Gene silencing of Lys c-1 mediated by RNA interference resulted in a significant reduction in the MPF activity compared with controls. The purified Lys c-1 protein reduced dopachrome formation by mosquito hemolymph phenoloxidase in solution assays in vitro. In vivo, Lys c-1 might inhibit melanization of beads by blocking attachment of critical factors to the bead surface or by inhibiting PO directly. This work indicates that insect lysozymes can play unexpected roles in mediating melanization of foreign targets.
Li
Bin
B
Department of Entomology, University of Wisconsin, 237 Russell Labs, 1630 Linden Dr, Madison, WI 53706, USA.
Paskewitz
Susan M
SM
D000818:Animals; D000852:Anopheles / Q000201:enzymology* / Q000235:genetics; D003911:Dextrans; D006458:Hemolymph / Q000737:chemistry; D008543:Melanins / Q000378:metabolism*; D008863:Microspheres; D009113:Muramidase / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D034622:RNA Interference
D003911:Dextrans; D008543:Melanins; C025614:sephadex; D009113:Muramidase