Distribution of neuropeptide FF-like immunoreactivity in the brain of the lizard Gekko gecko and its relation to catecholaminergic structures.
The present study provides a detailed description of the distribution of neuropeptide FF (NPFF)-like immunoreactivity in the brain of the lizard Gekko gecko. NPFF is found to be involved in nociception, cardiovascular regulation, and endocrine function. Because of its known relationship with catecholamines in mammals, double staining with tyrosine hydroxylase (TH) antibodies was used to corroborate this for reptiles. The present study revealed that NPFF-like-immunoreactive (NPFF-ir) cells and fibers were widely distributed throughout the brain. Major NPFF-ir cell groups were observed in the diagonal band nucleus of Broca, hypothalamus, and dorsal horn of the spinal cord. Additional cells were found in the anterior olfactory nucleus, lateral and dorsal cortices, dorsolateral septum, and diencephalic intergeniculate leaflet formation. Dense plexuses of NPFF-ir fibers were identified in the diagonal band nucleus of Broca, septum, preoptic and hypothalamic areas, isthmic region, ventrolateral tegmentum, solitary tract nucleus, and dorsolateral funiculus of the spinal cord. Extensive fiber staining also occurred in the nucleus accumbens and the midbrain tectum. Although an intimate relationship between NPFF-ir and TH-ir structures was obvious at many places in the brain, colocalization of these two substances was not observed. In conclusion, the distribution of NPFF in the brain of Gekko shares more features with anamniotes in terms of number of cell groups, more elaborate networks of fibers, and lack of colocalization with catecholamines than with mammals, suggesting a decrease in the distribution of this peptide in the latter vertebrate group.
Smeets
Wilhelmus J A J
WJ
Department of Anatomy, Vrije Universiteit Medical Center, 1081 BT Amsterdam, The Netherlands.
López
Jesús M
JM
González
Agustín
A
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism*; D001931:Brain Mapping; D002395:Catecholamines / Q000378:metabolism*; D007150:Immunohistochemistry; D008116:Lizards / Q000033:anatomy & histology* / Q000378:metabolism*; D009434:Neural Pathways / Q000033:anatomy & histology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D013116:Spinal Cord / Q000033:anatomy & histology / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D002395:Catecholamines; D009842:Oligopeptides; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide; D014446:Tyrosine 3-Monooxygenase