[Detecting the haplotype of three Y-STR loci by fluorescent multiplex amplification].
OBJECTIVE
To establish a fluorescent multiplex PCR system for typing Y-STR loci Y-GATA-A7.1, DYS456 and DYS443, and investigate their haplotype frequencies in Chinese Han population.


METHODS
203 unrelated males of Han population living in Zhengzhou were typed by fluorescent multiplex amplification system and ABI 3100 genetic analyzer.


RESULTS
In Zhengzhou Han population, 5,6 and 6 different alleles were observed for Y-GATA-A7.1, DYS456 and DYS443 loci, and their gene diversity (GD) were 0.669 2, 0.583 9 and 0.705 3 respectively. A total of 44 different haplotypes formed by these three loci was identified and the haplotype diversity (HD) reached 0.952 3.


CONCLUSIONS
The fluorescent multiplex system for these three Y-STR loci will be very powerful for forensic individual identification and paternity testing in Chinese Han population.
Lin
Xiao-Yan
XY
Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. linziily@yahoo.com.cn
Wang
Lei
L
Yuan
Bao-Long
BL
Feng
Chang-Jun
CJ
Huang
Dai-Xin
DX
D000483:Alleles; D002681:China / Q000208:ethnology; D041322:Chromosomes, Human, Y / Q000235:genetics*; D016172:DNA Fingerprinting; D017931:DNA Primers; D005453:Fluorescence; D005787:Gene Frequency; D005819:Genetic Markers; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D005819:Genetic Markers