A unique and universal molecular barcode array.
Molecular barcode arrays allow the analysis of thousands of biological samples in parallel through the use of unique 20-base-pair (bp) DNA tags. Here we present a new barcode array, which is unique among microarrays in that it includes at least five replicates of every tag feature. The use of smaller dispersed replicate features dramatically improves performance versus a single larger feature and allows the correction of previously undetectable hybridization defects.
Pierce
Sarah E
SE
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA.
Fung
Eula L
EL
Jaramillo
Daniel F
DF
Chu
Angela M
AM
Davis
Ronald W
RW
Nislow
Corey
C
Giaever
Guri
G
D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D020224:Expressed Sequence Tags; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation* / Q000379:methods; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D008969:Molecular Sequence Data; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016415:Sequence Alignment / Q000295:instrumentation* / Q000379:methods; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods
D015342:DNA Probes