Improving QTL mapping resolution based on genotypic sampling--a case using a RIL population.
The QTL mapping results were compared with the genotypically selected and random samples of the same size on the base of a RIL population. The results demonstrated that there were no obvious differences in the trait distribution and marker segregation distortion between the genotypically selected and random samples with the same population size. However, a significant increase in QTL detection power, sensitivity, specificity, and QTL resolution in the genotypically selected samples were observed. Moreover, the highly significant effect was detected in small size of genotypically selected samples. In QTL mapping, phenotyping is a more sensitive limiting factor than genotyping so that the selection of samples could be an attractive strategy for increasing genome-wide QTL mapping resolution. The efficient selection of samples should be more helpful for QTL maker assistant selection, fine mapping, and QTL cloning.
Yan
Jian-Bing
JB
National Maize Improvement Center of China, China Agricultural University, Beijing 100094, China. yjianbing@cau.edu.cn
Tang
Ji-Hua
JH
Meng
Yi-Jiang
YJ
Ma
Xi-Qing
XQ
Teng
Wen-Tao
WT
Chander
Subhash
S
Li
Lin
L
Li
Jian-Sheng
JS
D002874:Chromosome Mapping / Q000379:methods*; D005787:Gene Frequency / Q000235:genetics; D005821:Genetic Techniques; D018745:Genome, Plant / Q000235:genetics*; D005838:Genotype; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000235:genetics*