From phasing to structure refinement in-house: Cr/Cu dual-wavelength system and a loopless free crystal-mounting method.
The practical applicability of in-house structure determination using Cr K alpha X-rays (2.29 A) and a loopless free crystal-mounting method was examined using five novel proteins. Proteins from 9.6 to 84 kDa have been solved using this method without any derivatization. In all cases, more than 90% of structures were constructed automatically with side chains by use of the Cr SAD method. The free crystal-mounting technique increases the accuracy of the anomalous differences between Bijvoet mates and makes the in-house single-wavelength SAD method with a Cr K alpha X-ray source a very useful tool for high-throughput structure determination. In addition, a Cr/Cu dual-wavelength system makes it possible to perform structure analysis from phasing to refinement of the structure in-house.
Watanabe
Nobuhisa
N
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Japan. nobuhisa@sci.hokudai.ac.jp
D002857:Chromium / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray / Q000379:methods*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D002857:Chromium; D003300:Copper