title: Migratory dendritic cells transfer antigen to a lymph node-resident dendritic cell population for efficient CTL priming.
abstract: Skin dendritic cells (DCs) are thought to act as key initiators of local T cell immunity. Here we show that after skin infection with herpes simplex virus (HSV), cytotoxic T lymphocyte (CTL) activation required MHC class I-restricted presentation by nonmigratory CD8(+) DCs rather than skin-derived DCs. Despite a lack of direct presentation by migratory DCs, blocking their egress from infected skin substantially inhibited class I-restricted presentation and HSV-specific CTL responses. These results support the argument for initial transport of antigen by migrating DCs, followed by its transfer to the lymphoid-resident DCs for presentation and CTL priming. Given that relatively robust CTL responses were seen with small numbers of skin-emigrant DCs, we propose that this inter-DC antigen transfer functions to amplify presentation across a larger network of lymphoid-resident DCs for efficient T cell activation.
authors.affiliation: The Department of Microbiology and Immunology, The University of Melbourne, Parkville 3010, Victoria, Australia.
authors.forename: Rhys S
authors.initials: RS
authors.lastname: Allan
authors.forename: Jason
authors.initials: J
authors.lastname: Waithman
authors.forename: Sammy
authors.initials: S
authors.lastname: Bedoui
authors.forename: Claerwen M
authors.initials: CM
authors.lastname: Jones
authors.forename: Jose A
authors.initials: JA
authors.lastname: Villadangos
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhan
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Lew
authors.forename: Ken
authors.initials: K
authors.lastname: Shortman
authors.forename: William R
authors.initials: WR
authors.lastname: Heath
authors.forename: Francis R
authors.initials: FR
authors.lastname: Carbone
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D002465:Cell Movement; D002478:Cells, Cultured; D045142:Cross-Priming; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D006561:Herpes Simplex / Q000276:immunology / Q000821:virology; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D018139:Simplexvirus / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000941:Antigens