Simultaneous determination of fenbendazole, its sulphoxide and sulphone metabolites with the corresponding metabolites of triclabendazole in the plasma of sheep and cattle by high-performance liquid chromatography.
Pharmacokinetic studies following the simultaneous treatment of sheep or cattle with the anthelmintics triclabendazole and fenbendazole required an assay for determining the analytes in the plasma. An extraction and clean-up procedure is described which uses a C(18) Sep Pak cartridge, involves no evaporation steps and produces a clean extract from sheep and cattle plasma in which triclabendazole, fenbendazole and their respective oxidation products may be determined by reversed-phase high-performance liquid chromatography. Results from application of the method to a pharmacokinetic study of sheep are presented.
Bull
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CIBA-GEIGY Australia Limited, Western Road, Kemps Creek, N.S.W. 2171, Australia.
Shume
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GR