Esterase activity of Candida species isolated from immunocompromised hosts.
A total of 149 clinical isolates of Candida species isolated from immunocompromised patients were examined to ascertain their esterase activity by the Tween 80 opacity test, which is a biochemical test used mainly to differentiate between Candida albicans and Candida dubliniensis. Our results showed that C. albicans (92.3%), Candida tropicalis (92.3%), Candida parapsilosis (25%), C. dubliniensis (16.6%), Candida inconspicua (100%), and Candida lipolytica (100%) produced opacity halos through the 10-day post-inoculation period. The remaining Candida species did not produce a positive test response. These findings indicate that Tween 80 opacity test cannot be used as the sole phenotypic trait in the differentiation of C. albicans and C. dubliniensis.
Kumar
Chethrapilly Purushothaman Girish
CP
Department of Microbiology, Dr. A. L. Mudaliar Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India.
Menon
Thangam
T
Sundararajan
Thangavel
T
Nalini
Sarathy
S
Thirunarayan
Mandayam Anandampillai
MA
Rajasekaran
Sikhamani
S
Venkatadesikalu
Manjetti
M
D002175:Candida / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology*; D011232:Chemical Precipitation; D003470:Culture Media / Q000378:metabolism; D004950:Esterases / Q000032:analysis* / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D016867:Immunocompromised Host; D008027:Light; D010641:Phenotype; D011136:Polysorbates / Q000378:metabolism; D012542:Scattering, Radiation; D013045:Species Specificity
D003470:Culture Media; D005656:Fungal Proteins; D011136:Polysorbates; D004950:Esterases