title: Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment.
abstract: CLASP1 and CLASP2 are homologous mammalian proteins, which associate with the ends of growing microtubules, as well as the cell cortex and the kinetochores of mitotic chromosomes. Previous studies have shown that in interphase cells CLASPs can attach microtubule plus ends to the cortex and stabilize them by repeatedly rescuing them from depolymerization. Here we show that CLASP1 and 2 play similar and redundant roles in organizing the mitotic apparatus in HeLa cells. Simultaneous depletion of both CLASPs causes mitotic spindle defects and a significant metaphase delay, which often results in abnormal exit from mitosis. Metaphase delay is associated with decreased kinetochore tension, increased kinetochore oscillations and more rapid microtubule growth. We show that the association of CLASP2 with the kinetochores relies on its C-terminal domain, but is independent of microtubules or association with CLIP-170. We propose that CLASPs exhibit at the kinetochores an activity similar to that at the cortex, providing apparent stabilization of microtubules by locally reducing the amplitude of growth/shortening episodes at the microtubule ends. This local stabilization of microtubules is essential for the formation of normal metaphase spindle, completion of anaphase and cytokinesis.
authors.affiliation: KAN Research Institute, Kyoto Research Park, Kyoto 600-8815, Japan. y-kiyosue@kan.gr.jp
authors.forename: Yuko
authors.initials: Y
authors.lastname: Mimori-Kiyosue
authors.forename: Ilya
authors.initials: I
authors.lastname: Grigoriev
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Chiyuki
authors.initials: C
authors.lastname: Matsui
authors.forename: Anna
authors.initials: A
authors.lastname: Akhmanova
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Tsukita
authors.forename: Ivan
authors.initials: I
authors.lastname: Vorobjev
mesh_terms: D000818:Animals; D006367:HeLa Cells; D006801:Humans; D018386:Kinetochores / Q000378:metabolism*; D008677:Metaphase / Q000502:physiology; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis / Q000502:physiology; D009363:Neoplasm Proteins / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*; D014018:Tissue Distribution; D014981:Xenopus
chemical_list: C497850:CLASP1 protein, human; C497851:CLASP2 protein, human; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C076744:cytoplasmic linker protein 170