Isolation of Arabidopsis thaliana cDNAs that confer yeast boric acid tolerance.
An Arabidopsis thaliana cDNA library was introduced into a Saccharomyces cerevisiae mutant that lacks ScBOR1 (YNL275W), a boron (B) efflux transporter. Five cDNAs were identified that confer tolerance to high boric acid. The nucleotide sequence analysis identified the clones as a polyadenylate-binding protein, AtPAB2; a ribosomal small subunit protein, AtRPS20B; an RNA-binding protein, AtRBP47c'; and two Myb transcription factors, AtMYB13 and AtMYB68. The expression of these five genes also conferred boric acid tolerance on wild-type yeast. Two yeast genes, ScRPS20 and ScHRB1, that are similar to the isolated clones, were necessary for this boric acid tolerance. The possible roles of these A. thaliana and S. cerevisiae genes in boric acid tolerance are discussed.
Nozawa
Akira
A
Precursory Research for Embryonic Science and Technology, Kawaguchi, Saitama 332-0012, Japan.
Miwa
Kyoko
K
Kobayashi
Masaharu
M
Fujiwara
Toru
T
D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D001888:Boric Acids / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D015723:Gene Library; D026901:Membrane Transport Proteins; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
D001457:Anion Exchange Protein 1, Erythrocyte; D029681:Arabidopsis Proteins; C511808:Bor1 protein, S cerevisiae; D001888:Boric Acids; D018076:DNA, Complementary; D018744:DNA, Plant; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C032688:boric acid