title: Oxidative stress is closely related to clinical severity of pre-eclampsia.
abstract: Pre-eclampsia is a hypertensive disorder of pregnancy in which enzymatic antioxidant defenses fail and tissues are injured. This prospective case-control study evaluated whether pre-eclamptic women and their newborns show higher degrees of oxidative stress than normal pregnancies and sought to determine if this stress is related to clinical severity. Forty-four pre-eclamptic and thirty healthy pregnant women attending two hospitals in Valparaíso, Chile, were studied. The following plasmatic variables of antioxidant capacity were evaluated: glutathione peroxidase activity (GPx), total antioxidant capacity measured by oxidation of ABTS substrate (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), and superoxide dismutase activity (SOD). malondialdehide (MDA) was measured to evaluate lipoperoxidation. The evaluation was performed at diagnosis of pre-eclampsia, delivery, 30 days and 120 days post delivery. Newborns were studied at delivery through umbilical cord blood samples. Our analysis shows that antioxidant enzyme activity (SOD, GPx, ABTS) was significantly decreased, while lipoperoxidation (MDA) was increased in both pre-eclamptic groups compared to normal pregnant women (p<0.01). Statistically significant difference was found between mild and severe pre-eclamptic groups (p<0.01), for all biochemical markers studied. Therefore, the clinical severity of this pathology is closely related to the degree of oxidative stress.
authors.affiliation: Department of Gynecology and Obstetrics, School of Medicine, Universidad de Valparaíso, Chile. verónica.chamy@uv.cl
authors.forename: Verónica M
authors.initials: VM
authors.lastname: Chamy
authors.forename: Jaime
authors.initials: J
authors.lastname: Lepe
authors.forename: Alvaro
authors.initials: A
authors.lastname: Catalán
authors.forename: David
authors.initials: D
authors.lastname: Retamal
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Escobar
authors.forename: Eva M
authors.initials: EM
authors.lastname: Madrid
mesh_terms: D052160:Benzothiazoles / Q000097:blood / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005312:Fetal Blood / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D011225:Pre-Eclampsia / Q000175:diagnosis / Q000378:metabolism*; D011247:Pregnancy; D011446:Prospective Studies; D012720:Severity of Illness Index; D013451:Sulfonic Acids / Q000097:blood / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances
chemical_list: D052160:Benzothiazoles; D015415:Biomarkers; D013451:Sulfonic Acids; D017392:Thiobarbituric Acid Reactive Substances; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase