Comparison of exogenous glucose, fructose and galactose oxidation during exercise using 13C-labelling.
Six subjects exercised for 120 min on a cycle ergometer (65 (se 3) % VO2max) when ingesting a placebo or glucose, fructose or galactose (100 g in 1000 ml water) labelled with 13C. The oxidation of energy substrates including exogenous hexoses was compared using indirect respiratory calorimetry and 13CO2 production at the mouth. Total carbohydrate progressively decreased and total fat oxidation increased over the 120 min exercise period in the four experimental situations. During the 120 min of exercise, the amount of fructose oxidized (38.8 (se 2.6) g; 9.0 (se 0.6) % energy yield) was not significantly (approximately 4 %) lower than that of exogenous glucose (40.5 (se 3.4) g; 9.2 (se 0.8) % energy yield), while that of galactose (23.7 (se 3.5) g; 5.5 (se 0.9) % energy yield) was only 59 % and 61 % that of glucose and fructose, respectively. When compared with the placebo, the ingestion and oxidation of the three hexoses did not significantly modify fat oxidation or total carbohydrate oxidation, but it significantly reduced (9-13 %) endogenous carbohydrate oxidation. The present data indicate that fructose and exogenous glucose ingested during exercise could be oxidized at a similar rate, but that the oxidation rate of galactose was only approximately 60 % that of the exogenous glucose and fructose, presumably because of a preferential incorporation of galactose into liver glycogen (Leloir pathway). The reduction in endogenous carbohydrate oxidation was, however, similar with the three hexoses.
Burelle
Yan
Y
Department of Kinesiology, University of Montreal, CP 6128 Centre Ville, Montreal, Quebec, H3C 3J7, Canada.
Lamoureux
Marie-Catherine
MC
Péronnet
François
F
Massicotte
Denis
D
Lavoie
Carole
C
D000284:Administration, Oral; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D001944:Breath Tests; D002153:Calorimetry, Indirect / Q000379:methods; D002245:Carbon Dioxide / Q000502:physiology; D002247:Carbon Isotopes; D015444:Exercise / Q000502:physiology*; D005632:Fructose / Q000008:administration & dosage / Q000378:metabolism*; D005690:Galactose / Q000008:administration & dosage / Q000378:metabolism*; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D010084:Oxidation-Reduction
D001786:Blood Glucose; D002247:Carbon Isotopes; D007328:Insulin; D002245:Carbon Dioxide; D005632:Fructose; D005947:Glucose; D005690:Galactose