Capillary electrophoretic determination of thiamphenicol in turkeys serum and its pharmacokinetic application.
The pharmacokinetics of thiamphenicol were investigated in 12 healthy turkeys of both sexes, following intravenous (i.v.) and intragastric (p.o.) administration of a single dose of 30 mg/kg body weight (bw). Serum drug concentrations were determined by capillary electrophoresis technique on blood samples collected over 24 h following treatment. The method was statistically validated for its linearity, accuracy, precision and selectivity. The linear range was from 0.2 to 500 microg/ml with correlation coefficients greater than 0.999. The limit of detection of drug was 70 ng/ml, while the quantitative limit was 200 ng/ml, using 0.5 ml sample size. Pharmacokinetic variables of the drug were calculated after both administration routes.
Kowalski
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Medical University of Gdańsk, Faculty of Pharmacy, Hallera 107, PL-80-416 Gdańsk, Poland. piotrpl@wp.pl
D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D019540:Area Under Curve; D001682:Biological Availability; D019075:Electrophoresis, Capillary; D005260:Female; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D007441:Intubation, Gastrointestinal; D008297:Male; D012015:Reference Standards; D015203:Reproducibility of Results; D013839:Thiamphenicol / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D014422:Turkeys / Q000097:blood*
D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D013839:Thiamphenicol