Mono- and dicotyledonous plant-specific RNA editing sites are correctly edited in both in organello systems.
We set out to analyse the phylogenetic distribution of cox2 RNA editing sites. Database searches have revealed the presence of mono- and dicotyledonous-specific RNA editing sites. Therefore, to better understand tRNA editing system in plants, we developed a new dicotyledonous in organello RNA editing system using cauliflower mitochondria and analysed the transcription of the cox2 gene for both maize and Arabidopsis. These results were compared with those obtained from a maize mitochondrial in organello system. Surprisingly, both the mono- and dicotyledonous cox2 transcripts were efficiently edited in the mitochondrial cauliflower and maize in organello systems, respectively, even for RNA editing sites not present in the endogenous cox2 sequences. Taken together, our observations support a self-guiding-transcript model for RNA editing in higher plants.
Bolle
Nina
N
Abteilung Botanische Genetik und Molekularbiologie, Botanisches Institut und Botanischer Garten, Christian-Albrechts-Universität zu Kiel, Olshausenstrasse 40, 24098 Kiel, Germany.
Kempken
Frank
F
D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D001937:Brassica / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D050259:Genes, Mitochondrial; D017343:Genes, Plant; D019684:Magnoliopsida / Q000235:genetics*; D017393:RNA Editing; D016415:Sequence Alignment; D003313:Zea mays / Q000235:genetics
D003576:Electron Transport Complex IV