title: Simple identification of transgenic Arabidopsis plants carrying a single copy of the integrated gene.
abstract: Transgenic Arabidopsis thaliana plants carrying a single copy of integrated DNA can be identified by single-step genomic polymerase chain reaction. The reaction employs two sets of primer pairs with the same melting temperature that amplify the amplicons derived from the integrated T-DNA together with those from an endogenous single-copy gene as a reference. When the band intensity ratio is one, this means that the transgenic plants are carrying a single copy of the integrated gene per haploid.
authors.affiliation: Forestry Research Institute, Oji Paper, Kameyama, Mie 519-0212, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kihara
authors.forename: Cheng-Ri
authors.initials: CR
authors.lastname: Zhao
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Eiji
authors.initials: E
authors.lastname: Takita
authors.forename: Tetsu
authors.initials: T
authors.lastname: Kawazu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koyama
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D018628:Gene Dosage; D018745:Genome, Plant; D006579:Heterozygote; D006720:Homozygote; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction
chemical_list: D018744:DNA, Plant