title: Immunostimulating factor isolated from actinobacillus actinomycetemcomitans stimulates monocytes and inflammatory macrophages.
abstract: Immunostimulating factor (ISTF) isolated from Actinobacillus actinomycetemcomitans which has been described previously, is distinct from lipopolysaccharide and induces proliferation of B cells. This study was undertaken to investigate whether ISTF might enhance the stimulation of other immune cells. Immunohistochemically, ISTF exhibited a profound stimulating effect on macrophages and dendritic cells as well as B cells in the spleen of BALB/c mice. ISTF was also recognized for its capacity to induce direct activation of mouse macrophages to produce IL-6, TNF-alpha, and NO and MHC class II expression. Therefore, it is postulated that ISTF stimulates macrophages and possibly other cells to produce a wide variety of proinflammatory mediators, which may be involved in the chronicity and tissue destruction of periodontal disease.
authors.affiliation: Dong-A University Medical Science Research Center (BK21 Program), Busan, Korea.
authors.forename: Wol-Soon
authors.initials: WS
authors.lastname: Jo
authors.forename: Sung-Tae
authors.initials: ST
authors.lastname: Yee
authors.forename: Sik
authors.initials: S
authors.lastname: Yoon
authors.forename: Byung-Huouk
authors.initials: BH
authors.lastname: Nam
authors.forename: Eunju
authors.initials: E
authors.lastname: Do
authors.forename: Beom-Seok
authors.initials: BS
authors.lastname: Jung
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Jeong
authors.forename: Sook-Hee
authors.initials: SH
authors.lastname: Hong
authors.forename: Young-Hyun
authors.initials: YH
authors.lastname: Yoo
authors.forename: Chi-Dug
authors.initials: CD
authors.lastname: Kang
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Lim
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Jeong
authors.forename: Jae-Dong
authors.initials: JD
authors.lastname: Lee
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D004357:Drug Synergism; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D009569:Nitric Oxide / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D009569:Nitric Oxide