The role of endogenous histamine on the pathogenesis of the lipopolysaccharide (LPS)-induced, acute lung injury: a pilot study.
Histamine is widely distributed in the lungs and increases capillary permeability and P-selectin expression. To observe the role of histamine in acute lung injury (ALI), we measured the histamine and protein concentrations and cell numbers in the bronchoalveolar lavage (BAL) of LPS-induced ALI in rats. We instilled LPS (3 mg/kg) intratracheally, in conjunction with the intravenous histamine receptor antagonists (mepyramine, a H1-receptor antagonist, or ranitidine, a H2-receptor antagonist). LPS increased protein concentration and neutrophil numbers in the BAL as well as myeloperoxidase (MPO) activity in lungs after 6 h. LPS also increased histamine concentration in BAL after 2 h. Mepyramine and ranitidine attenuated the increased histamine concentrations. Total cell number in the BAL and MPO activity in the lungs were significantly decreased and neutrophil numbers and protein concentration in the BAL seemed to decrease with the administration of ranitidine at 6 h. In conclusion, endogenous histamine might be involved in the recruitment of neutrophils and protein leaks in LPS-induced ALI via the H2 receptors.
Kim
Tae-Hyung
TH
Department of Internal Medicine, Hanyang University College of Medicine, Seoul, South Korea.
Yoon
Ho Joo
HJ
Lim
Chae Man
CM
Kim
Eun-Kyung
EK
Kim
Mi Jung
MJ
Koh
Younsuck
Y
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D006632:Histamine / Q000378:metabolism*; D007249:Inflammation; D008070:Lipopolysaccharides / Q000378:metabolism*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury; D008297:Male; D009504:Neutrophils / Q000378:metabolism; D019007:P-Selectin / Q000096:biosynthesis; D009195:Peroxidase / Q000378:metabolism; D010865:Pilot Projects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011970:Receptors, Histamine H2 / Q000378:metabolism; D013997:Time Factors
D008070:Lipopolysaccharides; D019007:P-Selectin; D011970:Receptors, Histamine H2; D006632:Histamine; D009195:Peroxidase