title: The SIV tilted peptide induces cylindrical reverse micelles in supported lipid bilayers.
abstract: Elucidation of the molecular mechanism leading to biomembrane fusion is a challenging issue in current biomedical research in view of its involvement in controlling cellular functions and in mediating various important diseases. According to the generally admitted stalk mechanism described for membrane fusion, negatively curved lipids may play a central role during the early steps of the process. In this study, we used atomic force microscopy (AFM) to address the crucial question of whether negatively curved lipids influence the interaction of the simian immunodeficiency virus (SIV) fusion peptide with model membranes. To this end, dioleoylphosphatidylcholine/dipalmitoylphosphatidylcholine (DOPC/DPPC) bilayers containing 0.5 mol % dioleoylphosphatidic acid (DOPA) were incubated with the SIV peptide and imaged in real time using AFM. After a short incubation time, we observed a 1.9 nm reduction in the thickness of the DPPC domains, reflecting either interdigitation or fluidization of lipids. After longer incubation times, these depressed DPPC domains evolved into elevated domains, composed of nanorod structures protruding several nanometers above the bilayer surface and attributed to cylindrical reverse micelles. Such DOPC/DPPC/DOPA bilayer modifications were never observed with nontilted peptides. Accordingly, this is the first time that AFM reveals the formation of cylindrical reverse micelles in lipid bilayers promoted by fusogenic peptides.
authors.affiliation: Centre de Biophysique Moléculaire Numérique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés, 2, B-5030 Gembloux, Belgium.
authors.forename: Karim
authors.initials: K
authors.lastname: El Kirat
authors.forename: Yves F
authors.initials: YF
authors.lastname: Dufrêne
authors.forename: Laurence
authors.initials: L
authors.lastname: Lins
authors.forename: Robert
authors.initials: R
authors.lastname: Brasseur
mesh_terms: D008051:Lipid Bilayers / Q000138:chemical synthesis*; D008567:Membranes, Artificial; D008823:Micelles; D018625:Microscopy, Atomic Force / Q000379:methods; D015302:Simian Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics; D014760:Viral Fusion Proteins / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D008567:Membranes, Artificial; D008823:Micelles; D014760:Viral Fusion Proteins