The Hansenula polymorpha ATG25 gene encodes a novel coiled-coil protein that is required for macropexophagy.
We have isolated the Hansenula polymorpha ATG25 gene, which is required for glucose-induced selective peroxisome degradation by macropexophagy. ATG25 represents a novel gene that encodes a 45 kDa coiled-coil protein. We show that this protein colocalizes with Atg11 on a small structure, which most likely represents the pre-autophagosomal structure (PAS). In cells of a constructed ATG25 deletion strain (atg25) peroxisomes are constitutively degraded by nonselective microautophagy, a process that in WT H. polymorpha is only observed at nitrogen limitation conditions. This suggests that nonselective microautophagy is deregulated in H. polymorpha atg25 cells.
Monastyrska
Iryna
I
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.
Kiel
Jan A K W
JA
Krikken
Arjen M
AM
Komduur
Janet A
JA
Veenhuis
Marten
M
van der Klei
Ida J
IJ
D001343:Autophagy; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D020675:Peroxisomes / Q000378:metabolism / Q000648:ultrastructure; D010843:Pichia / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
D005656:Fungal Proteins