Effect of 1,2-indanedione on PCR-STR typing of fingerprints deposited on thermal and carbonless paper.
1,2-Indanedione treated fingerprints deposited on two substrates (thermal and carbonless paper) were swabbed and tested to determine the effect of the chemical on PCR-STR DNA typing. Samples from 10 post-treatment intervals were analyzed. Two extraction methods, Chelex and Qiamp, were used to determine the effect of extraction procedure on the quality of the DNA profiles. The LCN DNA samples were concentrated using Microcon 100 and amplified using the Profiler Plus amplification kit. 1,2-Indanedione did not adversely affect the DNA profiles obtained from the treated fingerprints. Partial DNA profiles were obtained at all post-development time frames. Both extraction methods produced comparable profiles although more "drop-ins" were observed with the Qiamp method. More "drop-outs" occurred in the DNA profiles from samples deposited on the thermal substrate compared with carbonless substrate.
Yu
Pei-Hua
PH
Department of Sciences, John Jay College of Criminal Justice, City University of New York, 445 West 59th Street, New York, NY, United States.
Wallace
Margaret M
MM
D000483:Alleles; D016172:DNA Fingerprinting / Q000295:instrumentation* / Q000379:methods*; D003878:Dermatoglyphics; D006801:Humans; D007189:Indans; D010209:Paper; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D013499:Surface Properties
C413384:1,2-indanedione; D007189:Indans