Neurite outgrowth activity of cyathane diterpenes from Sarcodon cyrneus, cyrneines A and B.
Two new cyathane diterpenes, cyrneines A (1) and B (2), were isolated from the mushroom Sarcodon cyrneus. The structures of the novel diterpenoids were determined by analysis of their spectroscopic data. Neither cyrneine A nor cyrneine B at 100 microM showed cytotoxicity as determined by LDH analysis. The stimulating activity on neurite outgrowth of cyrneines was evaluated. Rat pheochromocytoma cells (PC12), used as a model system of neuronal differentiation, were cultured with cyrneine A (100 microM), cyrneine B (100 microM) or NGF (100 ng/ml) for 24 h. Interestingly, cyrneines A and B significantly promoted neurite outgrowth in addition to NGF as a positive control.
Marcotullio
Maria Carla
MC
Dipartimento di Chimica e Tecnologia del Farmaco, Sez. Chimica Organica, Università degli Studi, Perugia, Italy. marcotu@unipg.it
Pagiott
Rita
R
Maltese
Federica
F
Obara
Yutaro
Y
Hoshino
Tomohiro
T
Nakahata
Norimichi
N
Curini
Massimo
M
D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006133:Growth Substances / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015394:Molecular Structure; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D019906:Nuclear Magnetic Resonance, Biomolecular; D016716:PC12 Cells; D051381:Rats
D004224:Diterpenes; D006133:Growth Substances; C514905:cyrneine A; C514906:cyrneine B; D007770:L-Lactate Dehydrogenase