title: Improved glutathione production by gene expression in Escherichia coli.
abstract: OBJECTIVE
  To improve glutathione (GSH) production in Escherichia coli by different genetic constructions containing GSH genes.
  
  
  RESULTS
  GSH production was very low in E. coli by the expression of gshI gene. An increase of GSH production was achieved by the expression of both gshI and gshII genes in E. coli. A higher GSH production, namely 34.8 mg g(-1) wet cell weight, was obtained by simultaneous expression of two copies of gshI gene and one copy of gshII gene.
  
  
  CONCLUSIONS
  The simultaneous expression of two copies of gshI gene and one copy of gshII gene resulted in a significant increase in GSH production.
  
  
  CONCLUSIONS
  The expression strategy for GSH production described here can be used to increase gene expression and obtain high production rates in other multienzyme reaction systems.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi, China.
authors.forename: X-Y
authors.initials: XY
authors.lastname: Liao
authors.forename: W
authors.initials: W
authors.lastname: Shen
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: G-C
authors.initials: GC
authors.lastname: Du
mesh_terms: D004926:Escherichia coli; D018628:Gene Dosage; D015870:Gene Expression; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis* / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D005974:Glutamate-Ammonia Ligase; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione