title: Cytotoxicity of a new root canal filling material on human gingival fibroblasts.
abstract: This study evaluated the cytotoxicity of the root canal sealing materials Resilon and Epiphany versus gutta-percha, Grossman's sealer, Thermaseal, and Sealapex. Using human gingival fibroblasts the fibroblasts cultures were incubated for either 1 or 24 h to test the cytotoxicity after freshly mixing or after 24 h of setting. Fibroblasts were then stained with trypan blue, to determine number of dead cells. Data were analyzed using ANOVA and t tests. Resilon was similar to gutta-percha and the control. Epiphany was less cytotoxic than Grossman's sealer at both the 1 and 24 h time periods. Epiphany was more cytotoxic than Sealapex at the 1-h time period but less cytotoxic at the 24 h time period. These results indicated that Resilon had a lower cytotoxicity and that Epiphany was more cytotoxic than conventional materials.
authors.affiliation: Department of Endodontics, University of Alabama at Birmingham School of Dentistry, Birmingham, Alabama 35294-0007, USA.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Key
authors.forename: Firoz G
authors.initials: FG
authors.lastname: Rahemtulla
authors.forename: Paul D
authors.initials: PD
authors.lastname: Eleazer
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D012387:Root Canal Filling Materials / Q000633:toxicity*; D013997:Time Factors
chemical_list: D012387:Root Canal Filling Materials; C486893:epiphany sealer; C486892:resilon sealer