title: In situ PCR protocols.
abstract: This chapter provides detailed methods and material descriptions of in situ polymerase chain reaction protocols. It describes all the essential components of the technique, various protocols suitable for different kinds of tissues and cell preparations, and also gives various instructions for troubleshooting. We have declined to devote detailed discussions on in situ hybridization because there are several excellent articles and books available on this subject.
authors.affiliation: Department of Biology, Claflin University, Orangeburg, SC, USA.
authors.forename: Omar
authors.initials: O
authors.lastname: Bagasra
authors.forename: Twaina
authors.initials: T
authors.lastname: Harris
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D004076:Digoxigenin / Q000378:metabolism; D005456:Fluorescent Dyes; D017403:In Situ Hybridization / Q000379:methods*; D009195:Peroxidase / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D011437:Propylamines / Q000737:chemistry; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D011437:Propylamines; D012821:Silanes; C088294:3-aminopropyltrimethoxysilane; D009195:Peroxidase; D000469:Alkaline Phosphatase; D004076:Digoxigenin