Strategies for drug metabolic profiling in human bile after administration of non-radioactive butoprozine.
A systematic approach to the recognition, isolation and identification of metabolites of butoprozine in human bile is described as an example of the general approach in this area. Human profiles are compared with those obtained after administration of 14C-labelled drug to the dog. The screening method is based on gradient-elution high-performance liquid chromatography (HPLC). Isolation is by isocratic reversed-phase HPLC and the identification procedure is performed using ultraviolet, mass and nuclear magnetic resonance spectroscopy.
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Department of Toxicology, State University, A. Deusinglaan 2, 9713 AW Groningen, The Netherlands.
de Zeeuw
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