[Sorcin overexpression and multidrug resistance in human gastric cancer cells].
OBJECTIVE
To screen multidrug resistance (MDR) related proteins in human gastric cancer using proteomics technique.


METHODS
Two-dimensional electrophoresis (2-DE) was used to separate the total proteins of vincristine-resistant human gastric cancer cell line SGC7901/VCR and its counterpart SGC7901. PDQuest software was used to analyze 2-DE images, and the differential expression proteins between the 2 cell lines were identified by both MALDI-TOF-MS and ESI-Q-TOF. The differential expression level of sorcin, one of the identified proteins, was confirmed by western blot analysis. The effect of sorcin on the development of MDR of SGC7901/VCR was determined by antisense oligonucleotides (ASO) technique.


RESULTS
Sorcin as a high expression protein in SGC7901/VCR was identified and the suppression of sorcin expression by sorcin ASO could enhance the vincristine chemosensitivity in SGC7901/VCR.


CONCLUSIONS
Sorcin overexpression is related to MDR in human gastric cancer cell line SGC7901/VCR.
Yi
Hong
H
Key Laboratory of Cancer Proteomics of Ministry of Health of China, Xiangya Hospital, Central South University, Changsha.
Yang
Yi-xuan
YX
Tang
Can-e
CE
Chen
Zhu-chu
ZC
Zhang
Gui-ying
GY
Xiao
Zhi-qiang
ZQ
D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008969:Molecular Sequence Data; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D014750:Vincristine / Q000494:pharmacology
D002135:Calcium-Binding Proteins; C503113:SRI protein, human; D014750:Vincristine