Estimation of plasma and saliva levels of coenzyme Q10 and influence of oral supplementation.
Coenzyme Q10 (CoQ(10)) levels in human saliva were measured by HPLC with a highly sensitive electrochemical detector (ECD) and a special concentration column. This HPLC system showed satisfactory analytical results within the standard range of 0.78-50 ng/ml. We also found a significant correlation between CoQ(10) levels in plasma and in saliva from parotid glands, while this correlation was lacking between plasma CoQ10 and CoQ10 in whole saliva. Unlike in plasma, there are some fluctuations of saliva CoQ(10) levels throughout the day. A good correlation was obtained by collecting parotid gland saliva at times between meals. The mean saliva CoQ(10) level for 55 healthy volunteers was 17.0 ng/ml (S.D. 6.8 ng/ml); approximately one fiftieth of that in plasma. Regarding the influence of oral supplementation, CoQ(10) was analyzed in plasma and parotid gland saliva from 20 healthy volunteers supplemented daily with 100 mg of CoQ(10) for the first week and 200 mg for the second. The plasma CoQ(10) levels of all volunteers increased to different extents in accordance with the CoQ(10) daily intake and the corresponding change in saliva showed almost the same trend.
Sekine
K
K
Research and Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Tokyo, Japan.
Ota
N
N
Nishii
M
M
Uetake
T
T
Shimadzu
M
M
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003067:Coenzymes; D006801:Humans; D010306:Parotid Gland / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*; D013375:Substance Withdrawal Syndrome / Q000097:blood; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood
D003067:Coenzymes; D014451:Ubiquinone; C024989:coenzyme Q10