Thiamine models and perspectives on the mechanism of action of thiamine-dependent enzymes.
Thiamine dependent enzymes catalyze ligase and lyase reactions near a carbonyl moiety. Chemical models for these reactions serve as useful tools to substantiate a detailed mechanism of action. This tutorial review covers all such studies performed thus far, emphasizing the role of each part around the active site and the conformation of the cofactor during catalysis.
Malandrinos
Gerasimos
G
University of Ioannina, Department of Chemistry, Ioannina, Greece.
Louloudi
Maria
M
Hadjiliadis
Nick
N
D001665:Binding Sites; D002384:Catalysis; D003067:Coenzymes / Q000737:chemistry; D008025:Ligases / Q000737:chemistry*; D008190:Lyases / Q000737:chemistry*; D008954:Models, Biological; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry; D013835:Thiamine Pyrophosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
D003067:Coenzymes; D009942:Organometallic Compounds; D008190:Lyases; D008025:Ligases; D013835:Thiamine Pyrophosphate