[Induction of protective immunity against Schistosoma japonicum by pcDNA3.1/Sj20.8 nucleic acid vaccine in BALB/c mice].
Sj20.8 gene was amplified by PCR and inserted into eukaryotic expression plasmid pcDNA3.1 to construct recombinant plasmid pcDNA3.1/Sj20.8, which was then injected into the quadriceps femoris of the BALB/c mice. Results showed that the Sj20.8 antigen was low expressed in the local tissue of the mice, and was not able to significantly reduce eggs in the liver than in the control mice.
Zeng
Qiao
Q
Institution of Pathogenic Biology, Nanhua University, Hengyang 421001, China.
Xiao
Jian-hua
JH
Wan
Zhi-gang
ZG
Liao
Li
L
Zhang
Yu-kuai
YK
Liu
Chuan-ai
CA
Yang
Qiu-lin
QL
D000818:Animals; D004274:DNA, Recombinant / Q000276:immunology; D005260:Female; D015723:Gene Library; D015801:Helminth Proteins / Q000235:genetics*; D007114:Immunization; D008099:Liver / Q000187:drug effects / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D010270:Parasite Egg Count; D010957:Plasmids / Q000235:genetics; D011897:Random Allocation; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D004274:DNA, Recombinant; D015801:Helminth Proteins; D019444:Vaccines, DNA