Evaluation of sample work-up methods and internal standards for the determination of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in brain by HPLC with electrochemical detection.
A high-performance liquid chromatographic method with electrochemical detection is described for the determination of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in brain. Results of studies on sample work-up methods and on the use of various internal standards are reported. The reproducibility of determination of 5-HT and 5-HIAA in two regions of rabbit brain has been evaluated.
Duchateau
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A
Department of Biochemistry, University of Limburg, B-3610 Diepenbeek, Belgium.
Martens
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H
Teuchy
H
H
Schoups
A
A
Claeys
M
M