The serodiagnosis of human infections with Yersinia enterocolitica and Yersinia pseudotuberculosis.
The techniques of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting were evaluated for the serodiagnosis of human infections with Yersinia enterocolitica and Yersinia pseudotuberculosis. Lipopolysaccharide (LPS) was prepared from strains comprising four serogroups of Y. enterocolitica and five serogroups of Y. pseudotuberculosis, tested against 200 sera submitted to the Laboratory of Enteric Pathogens for routine serodiagnosis, and shown to contain antibodies to Yersinia LPS by agglutination. Forty four sera were found to contain antibodies that bound to one of the LPS preparations used in the immunoassay. Thirty five of the sera contained antibodies to the LPS of Y. enterocolitica O3, whilst three contained antibodies to the LPS of Y. enterocolitica O5, 27 and Y. enterocolitica O9 LPS respectively. Two sera had antibodies to the LPS of Y. pseudotuberculosis II and a single serum contained antibodies to Y. pseudotuberculosis IV. The SDS-PAGE-immunoblotting procedure described proved to be a reliable procedure for the serodiagnosis of infections with Y. enterocolitica and Y. pseudotuberculosis.
Chart
Henrik
H
Laboratory of Enteric Pathogens, Centre for Infections, Health Protection Agency, London, UK. henrik.chart@hpa.org.uk
Cheasty
Thomas
T
D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D015009:Yersinia Infections / Q000097:blood / Q000175:diagnosis*; D015008:Yersinia enterocolitica / Q000276:immunology*; D015011:Yersinia pseudotuberculosis / Q000276:immunology*; D015012:Yersinia pseudotuberculosis Infections / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides