Estimation of the contribution of the bioluminescent reaction rate and quantum yield to the enhancement of firefly bioluminescence in the presence of cationic liposomes.
Cationic liposomes containing phosphatidylcholine, cholesterol and distearyldimethylammonium chloride (DSDAC) enhanced maximum light emission (BL intensity) and total light emission from the firefly bioluminescence (BL) reaction. The increase in BL intensity was interpreted on the basis of the increase in both BL reaction rate and BL quantum yield (PhiBL) of the BL reaction. The increase in BL reaction rate was due to the increase in the localized concentration of BL reactants on the surface of cationic liposomes by electrostatic interaction. On the other hand, the increase in PhiBL was due to the change of light-emitting species in the presence of cationic liposomes. Each contribution of BL reaction rate and PhiBL to the enhancement of the BL intensity was estimated by measuring the BL reaction rate and PhiBL in the presence of cationic liposomes containing various amounts of DSDAC. The contribution of the BL reaction rate to the increase in the BL intensity was found to be two-fold greater than that of PhiBL.
Kamidate
Tamio
T
Graduate School of Engineering, Hokkaido University, Kita-ku, Kita-13, Nishi-8, Sapporo 060-8628, Japan. kamidate@eng.hokudai.ac.jp
Tani
Hirofumi
H
Ishida
Akihiko
A
Hayashi
Masaya
M
D000818:Animals; D002412:Cations; D048888:Fireflies; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D008156:Luciferases / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
D002412:Cations; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; C514922:distearyldimethylammoniumchloride; D008156:Luciferases