title: [Relationship between the injury time and DNA fragmentation and its quantity in rat brain].
abstract: OBJECTIVE
  To observe the changes of DNA fragmentation and its quantity along with the time of injury in nerve cells after brain contusion.
  
  
  METHODS
  The model of brain contusion caused by free drop hammer was established. TUNEL and Feulgen's DNA staining conjoined with image analysis technique were used for exploration.
  
  
  RESULTS
  With the gradually rising of DNA fragmentation, DNA quantity was declining in the brain tissue after contusion.
  
  
  CONCLUSIONS
  TUNEL and Feulgen's DNA staining conjoined with image analysis technique could be utilized in the timing of brain injury and provide a new approach for this issue.
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. fanlfy@mail.haust.edu.cn
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liao
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Ma
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Guan
authors.forename: Min
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology*; D004247:DNA / Q000032:analysis*; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D049429:Forensic Pathology; D020287:In Situ Nick-End Labeling / Q000379:methods*; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
chemical_list: D004247:DNA