Core oligosaccharide structure from the highly phytopathogenic Agrobacterium tumefaciens TT111 and conformational analysis of the putative rhamnan epitope.
The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares structural features with other polysaccharides from well-known plant pathogenic bacteria. Its conformation was determined through extensive molecular dynamic (MD) analysis and presents an epitope similar to that recognized from the plant defense system.
De Castro
Cristina
C
Department of Organic Chemistry and Biochemistry, University of Naples, Complesso Universitario Monte Sant' Angelo, Via Cintia 4, 80126 Napoli, Italy. decastro@unina.it
Carannante
Anna
A
Lanzetta
Rosa
R
Liparoti
Valeria
V
Molinaro
Antonio
A
Parrilli
Michelangelo
M
D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D002240:Carbohydrate Sequence; D003837:Deoxy Sugars / Q000737:chemistry* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology; D012210:Rhamnose / Q000737:chemistry* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003837:Deoxy Sugars; D008070:Lipopolysaccharides; D008351:Mannans; D009844:Oligosaccharides; C065836:rhamnan; D012210:Rhamnose