Interleukin-10 inhibits gram-negative-microbe-specific human receptor activator of NF-kappaB ligand-positive CD4+-Th1-cell-associated alveolar bone loss in vivo.
To study anti-inflammatory cytokine effects on RANKL+-T-cell-mediated osteoclastogenesis in vivo, we injected human interleukin-10 (hIL-10) into pathogen-infected HuPBL-NOD/SCID mice. The results show significantly decreased RANKL+ Th1-associated alveolar bone loss and coexpression of human gamma interferon (hIFN-gamma) and human macrophage colony-stimulating factor, but not hIL-4, in RANKL+ Th cells compatible with those from successfully treated aggressive periodontitis subjects. Thus, there are critical cytokine interactions linking hIFN-gamma+ Th1 cells to RANKL-RANK/OPG signaling for periodontal osteoclastogenesis in vivo.
Zhang
Xiaoxia
X
Laboratory of Molecular Microbial Immunity and Division of Peridontology, Eastman Department of Dentistry and Centre for Oral Biology, University of Rochester, Rochester, New York 14620, USA.
Teng
Yen-Tung A
YT
D000189:Actinobacillus Infections / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology; D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology / Q000472:pathogenicity*; D016301:Alveolar Bone Loss / Q000276:immunology* / Q000503:physiopathology; D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010518:Periodontitis / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism*
D015704:CD4 Antigens; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; C505686:TNFRSF11A protein, human; C505688:TNFSF11 protein, human; C505687:Tnfrsf11a protein, mouse; C505689:Tnfsf11 protein, mouse; D016753:Interleukin-10; D007371:Interferon-gamma