title: The CXXC motif at the N terminus of an alpha-helical peptide.
abstract: An active site containing a CXXC motif is always found in the thiol-disulphide oxidoreductase superfamily. A survey of crystal structures revealed that the CXXC motif had a very high local propensity (26.3 +/- 6.2) for the N termini of alpha-helices. A helical peptide with the sequence CAAC at the N terminus was synthesized to examine the helix-stabilizing capacity of the CXXC motif. Circular dichroism was used to confirm the helical nature of the peptide and study behavior under titration with various species. With DTT, a redox potential of E(o) = -230 mV was measured, indicating that the isolated peptide is reducing in nature and similar to native human thioredoxin. The pK(a) values of the individual Cys residues could not be separated in the titration of the reduced state, giving a single transition with an apparent pK(a) of 6.74 (+/-0.06). In the oxidized state, the N-terminal pK(a) is 5.96 (+/-0.05). Analysis of results with the modified helix-coil theory indicated that the disulfide bond stabilized the alpha-helical structure by 0.5 kcal/mol. Reducing the disulfide destabilizes the helix by 0.9 kcal/mol.
authors.affiliation: Manchester Interdisciplinary Biocentre, The University of Manchester, UK.
authors.forename: Teuku M
authors.initials: TM
authors.lastname: Iqbalsyah
authors.forename: Efrosini
authors.initials: E
authors.lastname: Moutevelis
authors.forename: Jim
authors.initials: J
authors.lastname: Warwicker
authors.forename: Neil
authors.initials: N
authors.lastname: Errington
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Doig
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002942:Circular Dichroism; D003198:Computer Simulation; D003545:Cysteine / Q000737:chemistry*; D004229:Dithiothreitol / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D017510:Protein Folding; D017433:Protein Structure, Secondary; D013879:Thioredoxins / Q000737:chemistry*
chemical_list: D013879:Thioredoxins; D003545:Cysteine; D004229:Dithiothreitol