Differential effects of embryo transfer and maternal factors on anxiety-related behavior and numbers of neuropeptide Y (NPY) and parvalbumin (PARV) containing neurons in the amygdala of inbred C3H/HeN and DBA/2J mice.
The effects of reciprocal embryo transfers were studied on anxiety-related behavior of inbred C3H/HeN and DBA/2J mice on the elevated plus maze (EPM), and related to amygdaloid neuropeptide Y (NPY)- and parvalbumin (PARV)-immunoreactive neurons. Embryo transfer significantly reduced closed arm entries in in-stain-transferred C3H mice, and maternal factors influenced open arm entries only in interaction with genetic background and sex. In DBA/2J-mice, embryo transfer resulted in a reduced number of NPY-immunoreactive (NPY-ir) neurons, while PARV-immunoreactive (PARV-ir) cells were not affected. In C3H/HeN mice, however, in-strain embryo transfer only resulted in a reduction of the number of PARV-immunoreactive neurons. Maternal factors mainly induced changes in the number of NPY-ir neurons in the basolateral complex of the amygdala either directly or in interaction with genetic factors. In summary, in-strain embryo transfer had a minor effect on the behavior of C3H/HeN mice, and a differential influence on the numbers of amygdaloid NPY-ir and PARV-ir neurons of inbred C3H/HeN and DBA/2J mice. Maternal factors had a stronger impact on the numbers of NPY-ir neurons than PARV-ir neurons. The present results indicate that alterations in behavior and amygdala morphology induced by embryo transfer or maternal factors depend on the genetic background of the mouse strains used.
Rose
Claudia
C
Institut für Anatomie, Section Neuroanatomy, Otto-von-Guericke Universität, Haus 43, Leipziger Street 44, D-39120 Magdeburg, Germany. Claudia.Rose1@gmx.de
Schwegler
Herbert
H
Hanke
Joachim
J
Röhl
Friedrich-Wilhelm
FW
Yilmazer-Hanke
Deniz M
DM
D000679:Amygdala / Q000378:metabolism* / Q000473:pathology; D000704:Analysis of Variance; D000818:Animals; D001007:Anxiety / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D004624:Embryo Transfer; D005106:Exploratory Behavior / Q000502:physiology; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D013045:Species Specificity
D009478:Neuropeptide Y; D010320:Parvalbumins