title: Some aspects of the venom proteome of the Colubridae snake Philodryas olfersii revealed from a Duvernoy's (venom) gland transcriptome.
abstract: We investigated the putative toxins of Philodryas olfersii (Colubridae), a representative of a family of snakes neglected in venom studies despite their growing medical importance. Transcriptomic data of the venom gland complemented by proteomic analysis of the gland secretion revealed the presence of major toxin classes from the Viperidae family, including serine proteases, metalloproteases, C-type lectins, Crisps, and a C-type natriuretic peptide (CNP). Interestingly, the phylogenetic analysis of the CNP precursor showed it as a linker between two related precursors found in Viperidae and Elapidae snakes. We suggest that these precursors constitute a monophyletic group derived from the vertebrate CNPs.
authors.affiliation: Centro de Biotecnologia, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo, SP 05503-900, Brazil.
authors.forename: Ana T C
authors.initials: AT
authors.lastname: Ching
authors.forename: Marisa M T
authors.initials: MM
authors.lastname: Rocha
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Paes Leme
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Pimenta
authors.forename: Maria
authors.initials: M
authors.lastname: de Fátima D Furtado
authors.forename: Solange M T
authors.initials: SM
authors.lastname: Serrano
authors.forename: Paulo L
authors.initials: PL
authors.lastname: Ho
authors.forename: Inácio L M
authors.initials: IL
authors.lastname: Junqueira-de-Azevedo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017812:Colubridae / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D017815:Elapidae / Q000145:classification; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags / Q000737:chemistry; D005260:Female; D037181:Lectins, C-Type / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D008297:Male; D045726:Metalloproteases / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides / Q000737:chemistry / Q000145:classification / Q000235:genetics; D009842:Oligopeptides / Q000737:chemistry / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D011498:Protein Precursors / Q000737:chemistry / Q000145:classification / Q000235:genetics; D020543:Proteome / Q000737:chemistry / Q000145:classification / Q000235:genetics; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D012910:Snake Venoms / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D014158:Transcription, Genetic; D017819:Viperidae / Q000145:classification
chemical_list: D037181:Lectins, C-Type; D045265:Natriuretic Peptides; D009842:Oligopeptides; D011498:Protein Precursors; D020543:Proteome; D012910:Snake Venoms; C006791:bradykinin potentiating factors; D045726:Metalloproteases; D012697:Serine Endopeptidases