New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells.
We present a new approach for biomass assessment in cell culture using a disposable microcentrifuge tube. The specially designed tube is fitted with an upper chamber for sample loading and a lower 5 microL capillary for cell collection during centrifugation. The resulting packed cell volume (PCV) can be quantitatively expressed as the percentage of the total volume of the sample. The present study focused on the validation of the method with mammalian cell lines that are widely used in bioprocessing. Using several examples, the PCV method was shown to be more precise, rapid, and reproducible than manual cell counting.
Stettler
Matthieu
M
Ecole Polytechnique Fédérale de Lausanne, Faculty of Life Sciences, Institute of Bioengineering, Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.
Jaccard
Nicolas
N
Hacker
David
D
De Jesus
Maria
M
Wurm
Florian M
FM
Jordan
Martin
M
D000818:Animals; D018533:Biomass; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D006224:Cricetinae; D005069:Evaluation Studies as Topic; D006801:Humans; D051379:Mice