[Experimental study of the inhibitory effects of Chelidonium majus L. extractive on Streptococcus mutans in vitro].
OBJECTIVE
To study the inhibitory effects of Chelidonium majus L. extractive on the growth of Streptococcus mutans in vitro, and to explore its mechanism in caries prevention.


METHODS
Streptococcus mutans 25175 was chosen as the experimental bacterium. The Chelidonium majus L. extractives chelidonine and chelerythrine were double diluted to different concentrations by two-fold dilution. The inhibitory effect of Streptococcus mutans was measured by slip diffusion method. The minimal inhibitory concentration(MIC) was also determined. 0.16% liquor hibitane was used as positive control. Spearman correlation was used for statistical analysis.


RESULTS
Inhibition zone of Streptococcus mutans appeared in some concentration of chelerythrine, but no inhibition zone in each concentration of chelidonine. The MIC of chelerythrine was 0.78 mg/ml which determined by liquid culture medium. The concentration of chelerythrine was highly related to the inhibitory zone of Streptococcus mutans (r=0.99, P<0.01).


CONCLUSIONS
The antibacterial activity of Chelidonium majus L. extractive chelerythrine on Streptococcus mutans was significant,and the antibacterial activity of the concentration 100 mg/ml was higher than that of 0.16% liquor hibitane (19.4 mm), indicating that chelerythrine can be used as an agent for prevention of dental caries.
Cheng
Rui-bo
RB
School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China. chengruibo@sohu.com
Chen
Xu
X
Liu
Shu-jie
SJ
Zhang
Xiao-fang
XF
Zhang
Guang-he
GH
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D053119:Benzophenanthridines / Q000494:pharmacology*; D028281:Chelidonium / Q000737:chemistry*; D003731:Dental Caries / Q000517:prevention & control; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D053119:Benzophenanthridines; D010936:Plant Extracts; C062047:chelidonine; C016299:chelerythrine