Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes: an AFM and DLS comparative study.
Atomic force microscopy (AFM) is used to describe the formation process of polymer/DNA complexes. Two main objectives of this research are presented. The first one is to apply AFM as an effective tool to analyse DNA molecules and different polycation/DNA complexes in order to evaluate their degree of condensation (size and shape). The other one is to search for a relationship between the condensation state of DNA and its transfection efficiency. In this study, linear methacrylate based polymers and globular SuperFect polymers are used in order to induce DNA condensation. Ternary complexes, composed of methacrylate based polymers and polyethylene glycol (PEG)-based copolymers, are also investigated. AFM allows us to confirm good condensation conditions and relate them (or not) to transfection efficiencies. These AFM results (obtained after drying in air) are compared with measurements deduced from Dynamic Light Scattering (DLS) experiments performed in water. This comparison allowed us to identify the structural modifications resulting from deposition on the mica surface.
Volcke
C
C
Laboratoire de Spectroscopie Moléculaire de Surface (LASMOS), University of Namur, Belgium. cedric.volcke@fundp.ac.be
Pirotton
S
S
Grandfils
Ch
Ch
Humbert
C
C
Thiry
P A
PA
Ydens
I
I
Dubois
P
P
Raes
M
M
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D008027:Light; D008689:Methacrylates / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012542:Scattering, Radiation; D014162:Transfection / Q000379:methods*
D008689:Methacrylates; D011108:Polymers; D011092:Polyethylene Glycols; D004247:DNA