[Investigation of viral pathogens contributing to habitual abortion].
OBJECTIVE
To investigate the association of Coxsackie B virus (CBV) with habitual abortion.


METHODS
CBV IgM antibody, viral RNA and virions were detected in 86 women with habitual abortion and 40 with induced abortion by means of enzyme-linked immunosorbent assay (ELISA), RT-PCR and virus isolation, respectively.


RESULTS
The positivity rate of CBV IgM were 87.2% and 35% in the two groups, respectively, and the detection rate of the viral RNA was 53.5% and 17.5% in blood lymphocytes, and 59.3% and 17.5% in the placentas. The virions were found in the placentas in 41.9% and 6.9% of the women, respectively. The positivity rates of CBV IgM, viral DNA and virions showed significant difference between the two groups (P<0. 01).


CONCLUSIONS
CBV might be one of the causes responsible for habitual abortion.
Jiang
Wen-ling
WL
Medical Reseach Center, People's Hospital of Guangdong Province, Guangzhou 510080, China. jwenling@126.com
Liu
Zhao
Z
Yang
Zhan-qiu
ZQ
Xu
Shun-xian
SX
Luo
Xian-ling
XL
D000026:Abortion, Habitual / Q000097:blood / Q000209:etiology*; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D003384:Coxsackievirus Infections / Q000150:complications* / Q000821:virology; D029822:Enterovirus B, Human / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D008214:Lymphocytes / Q000648:ultrastructure / Q000821:virology; D008854:Microscopy, Electron; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000097:blood / Q000821:virology*; D012367:RNA, Viral / Q000097:blood
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D012367:RNA, Viral