[DNA genotyping of oral epithelial cells by laser capture microdissection].
OBJECTIVE
The STR genotypping of trace oral epithelial cells which are microdissected by laser capture microdissection system (LCM) is explored.


METHODS
The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures.


RESULTS
DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed.


CONCLUSIONS
It is viable in genotyping of trace oral epithelial cells by Laser Capture Microdissection as a new technology of seperating single cell.
Gu
Li-Hua
LH
Shanghai Institute of Forensic Science, Shanghai, 200083, China.
Zhang
Cheng
C
Chen
Lian-Kang
LK
Zhen
Hui-Fen
HF
Cheng
Li
L
Zhou
Huai-Gu
HG
D002469:Cell Separation / Q000379:methods; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004847:Epithelial Cells; D005838:Genotype; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000295:instrumentation / Q000379:methods*; D009055:Mouth / Q000166:cytology*; D020080:Tandem Repeat Sequences
D004247:DNA