Acetylcholinesterase activity in rats experimentally demyelinated with ethidium bromide and treated with interferon beta.
The ethidium bromide (EB) demyelinating model was associated with interferon beta (IFN-beta) to evaluate acetylcholinesterase (AChE) activity in the striatum (ST), hippocampus (HP), cerebral cortex (CC), cerebellum (CB), hypothalamus (HY), pons (PN) and synaptosomes from the CC. Rats were divided into four groups: I control (saline), II (IFN-beta), III (EB) and IV (EB and IFN-beta). After 7, 15 and 30 days rats (n = 6) were sacrificed, and the brain structures were removed for enzymatic assay. AChE activity was found to vary in all the brain structures in accordance with the day studied (7-15-30 days) (P < 0.05). In the group III, there was an inhibition of the AChE activity in the ST, CB, HY, HP and also in synaptosomes of the CC (P < 0.05). It was observed that IFN-beta per se was capable to significantly inhibit (P < 0.05) AChE activity in the ST, HP, HY and synaptosomes of the CC. Our results suggest that one of the mechanisms of action of IFN-beta is through the inhibition of AChE activity, and EB could be considered an inhibitor of AChE activity by interfering with cholinergic neurotransmission in the different brain regions.
Mazzanti
C M
CM
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcellos, 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil.
Spanevello
R M
RM
Pereira
L B
LB
Gonçalves
J F
JF
Kaizer
R
R
Corrêa
M
M
Ahmed
M
M
Mazzanti
A
A
Festugatto
R
R
Graça
D L
DL
Morsch
V M
VM
Schetinger
M R C
MR
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology*; D002800:Cholinesterase Inhibitors / Q000378:metabolism; D003711:Demyelinating Diseases / Q000139:chemically induced*; D004996:Ethidium / Q000633:toxicity*; D016899:Interferon-beta / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000201:enzymology
D002800:Cholinesterase Inhibitors; D016899:Interferon-beta; D000110:Acetylcholinesterase; D004996:Ethidium