Investigating the interactions of hyaluronan derivatives with biomolecules. The use of diffusional NMR techniques.
[Chemical structure: see text] The interactions between a biomaterial and biomolecules present in body fluids often determine the fate of the biomaterial. This paper presents a study on hyaluronan (HA)-containing materials (in soluble or colloidal form) that focuses on their interactions with lipids and proteins and for the first time uses PFG NMR as an analytical technique for probing these events. The interactions of HA-based polymers with phospholipids (DPPC and DPPG liposomes) are shown to depend both on charge and hydrophobicity factors. Despite the difference in behavior between albumin (substantially non-adhesive) and fibrinogen (adhesive), the interactions of the polymers with proteins do not seem to be based on hydrophobic effects but on surface polar interactions.
Taglienti
Anna
A
Department of Chemistry, University "La Sapienza", Piazzale Aldo Moro 5, 00185 Rome, Italy.
Cellesi
Francesco
F
Crescenzi
Vittorio
V
Sequi
Paolo
P
Valentini
Massimiliano
M
Tirelli
Nicola
N
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D008081:Liposomes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry
D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D012710:Serum Albumin, Bovine; D005340:Fibrinogen; D006820:Hyaluronic Acid