Purification and characterization of cellobiose dehydrogenase from white-rot basidiomycete Trametes hirsuta.
In order to save energy during the pulp making process, we tried to use white-rot basidiomycete, Trametes hirsuta, which degrades lignin efficiently. But a decrease in paper strength caused by cellulolytic activity ruled this out for practical application. Since the cellulolytic activity of the fungus must be decreased, we purified and characterized a cellobiose dehydrogenase (CDH) that was reported to damage pulp fiber. The CDH in the culture filtrate of C. hirsutus was purified by freeze-thawing and chromatographic methods. The pI of the enzyme was 4.2 and its molecular weight was 92 kDa. The optimal temperature and pH of the enzyme were 60-70 degrees C and 5.0 respectively. Since the purified CDH decreased the viscosity of pulp in the presence of Fe(III) and cellobiose, it was shown that the suppression of CDH should be an effective way to reduce cellulose damage.
Nakagame
Seiji
S
Technological Initiatives Research Laboratory, Oji Paper Co., Ltd., Tokyo 135-8558, Japan.
Furujyo
Atsushi
A
Sugiura
Jun
J
D002237:Carbohydrate Dehydrogenases / Q000737:chemistry* / Q000302:isolation & purification; D002475:Cellobiose / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry; D020072:Polyporales / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
D002475:Cellobiose; D002482:Cellulose; D007501:Iron; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase