[A comparative study on isoenzymes of Sarcocystis spp. from cattle and water buffaloes].
OBJECTIVE
To analyze the zymogram of peroxidase (PER) and phosphoglucose isomerase (PGI) of three species of Sarcocystis.


METHODS
The collected parasites were homogenized and fragmented by ultrasonication. After centrifugation, the supernatants were analyzed by isoelectric focusing electrophoresis.


RESULTS
The isolates of S. cruzi from infected water buffalo and cattle all showed identical enzyme profiles, 7 bands of PER at pH 4.44-6.98 and 6 bands of PGI at pH 4.66-6.53; and same with the isolates of S. hirsuta. 5 bands of PER at pH 4.97-7.15 and 4 bands of PGI at pH 4.70-6.51. The zymograms among S. cruzi, S. hirsuta and S. fusiformis were different considerably.


CONCLUSIONS
The data support the hypothesis that both water buffalo and cattle are the natural intermediate hosts of S. cruzi and S. hirsuta at the gene level. S. cruzi, S. hirsuta and S. fusiformis are different species.
Liang
Yi-long
YL
Bioinformatics College, Chongqing University of Posts and Communications, Chongqing 400065, China.
Xie
Yong-fang
YF
Zuo
Yang-xian
YX
Tan
De-yong
DY
Shu
Kun-xian
KX
Chen
Xin-wen
XW
D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D002417:Cattle / Q000469:parasitology*; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism; D012522:Sarcocystis / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D013045:Species Specificity
D007527:Isoenzymes; D015800:Protozoan Proteins; D009195:Peroxidase; D005956:Glucose-6-Phosphate Isomerase