[Isolation, identification of lignin-degrading bacteria and purification of lignin peroxidase].
Two strains that could use lignin as sole carbon source and excrete peroxidases were isolated from activated sludge. Both strains are belonged to Pseudomanas based on morphological, physio-biochenical characterizatics and homology identification of 16S rDNA sequence, in which strain PKE117 is identified as a new species, while the strain PKE225 is identified as Pseudomanas thermaerum. Crude enzyme in liquid fermentation of PKE117 was analyzed with DEAE-cellulose 32 ion-exchanger resin chromatography and Sephadex G-75 gel-filtration in proper order. The lignin peroxidase specific activity increases from 0.87 U/mg to 204.5 U/mg, purification multiple is 235.1 and callback rate is 15%.
Yang
Jin-shui
JS
The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University, Beijing 100871, China. yangjsh1999@163.com
Liu
Wei
W
Ni
Jin-ren
JR
D008031:Lignin / Q000378:metabolism; D010544:Peroxidases / Q000302:isolation & purification*; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification*; D012722:Sewage / Q000382:microbiology*
D012722:Sewage; D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase