title: Cortical expression of nuclear factor kappaB after human brain contusion.
abstract: The aim of current study was to analyze the binding activity and the temporal and cellular expression of nuclear factor kappa B (NF-kappaB) in human contused brain. Eighteen contused brain samples were obtained from 17 patients undergoing surgery for brain contusions 5-80 h after trauma. NF-kappaB binding activity was detected by electrophoretic mobility shift assay (EMSA), and temporal and cellular expression of NF-kappaB subunits p65 and p50 was analyzed by immunohistochemistry. The results showed that a progressive upregulation of NF-kappaB activity occurred in the area surrounding the injured brain with the time from brain trauma to operation. The maximal expression of NF-kappaB was detected after 48 h postinjury. The expression of NF-kappaB p65 was mainly located at glial and vascular endothelial cells without expression at neurons. The expression of NF-kappaB p50 was mainly located at glial cells, a little at neurons and no expression at vascular endothelial cells. Within 24 h postinjury, both NF-kappaB p65 and p50 immunoreactivity was mainly observed in the nucleus of cells. After 24 h postinjury, NF-kappaB p65 labeling was found in the both nucleus and cytoplasm of glial and endothelial cells; otherwise, p50 labeling was primarily found in the nucleus of glial cells and in the nucleus, cytoplasm and process of neurons. It is concluded that NF-kappaB could be highly upregulated at human contused brain and the cellular pattern of p65 and p50 expression might be closely associated with the cell functions.
authors.affiliation: Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing 210002, Jiangsu Province, P.R. China. hang1965@public1.ptt.js.cn
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Hang
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Shi
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jing-Song
authors.initials: JS
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D001924:Brain Concussion / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism; D013997:Time Factors
chemical_list: D016328:NF-kappa B; D021122:Protein Subunits