D609 blocks cell survival and induces apoptosis in neural stem cells.
In order to investigate the effects of tricyclodecane-9-yl-xanthogenate (D609) on the survival of neural stem cells (NSCs), which were isolated from rat forebrain, we treated the NSCs with D609 in the presence of basic fibroblast growth factor (bFGF). We found that when NSCs were exposed to 18.76-56.29 microM D609, the viability of the cells remarkably declined and apoptosis occurred. At the same time, the ROS level in NSCs was depressed. The data suggested that D609 was a powerful growth inhibitor and apoptosis inducer in NSCs.
Wang
Nan
N
Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
Lv
Xin
X
Su
Le
L
Zhao
BaoXiang
B
Zhang
ShangLi
S
Miao
JunYing
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006836:Hydro-Lyases / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D009636:Norbornanes; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology*
D001952:Bridged-Ring Compounds; D009636:Norbornanes; D017382:Reactive Oxygen Species; D013859:Thiocarbamates; D013871:Thiones; C046498:tricyclodecane-9-yl-xanthogenate; D006836:Hydro-Lyases; C045581:lactate dehydratase