Kinetic preference for interaction of cisplatin with the G-C-rich wobble basepair region in both tRNAAla and MhAla.
The anticancer active complex cisplatin interacts preferentially with the common, G-C rich, wobble base pair region of both tRNA(Ala) and Mh(Ala) in a reaction that at pH 6.3 is rate limited by the acid hydrolysis of the metal complex.
Papsai
Pal
P
Biochemistry, Chemical Center, Lund University, SE-221 00 Lund, Sweden.
Aldag
Jasmin
J
Persson
Tina
T
Elmroth
Sofi K C
SK
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001345:Autoradiography; D020029:Base Pairing; D002945:Cisplatin / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D007700:Kinetics; D012313:RNA / Q000737:chemistry* / Q000187:drug effects*; D012344:RNA, Transfer, Ala / Q000737:chemistry / Q000378:metabolism*
D000970:Antineoplastic Agents; D012344:RNA, Transfer, Ala; D012313:RNA; D002945:Cisplatin