[Observation the electroporation of blood cells in vitro by scanning electron microscope].
OBJECTIVE
To observe changes on cell membrane in blood cells after they were been electrified.


METHODS
Blood were electrified for 5, 10, 20, 30 s, 1 min respectively, and Scanning electron microscope was used to detect the changes on their cell membranes.


RESULTS
Pores were detected both on electrified erythrocytes and leukocytes with round or ellipse shapes. The erythrocytes often have one or more pores while the leukocytes often have more pores looked like cribble. The rates of perforated cells were increased with the prolonging time of electrification, 5 s with 6% and 1 min increased to 40%.


CONCLUSIONS
Alternating current can cause the cell perforating, and the rates of perforated cell were increased with the prolonging time of electrification.
Wang
Ye
Y
West China Preclinical Medicine and Forensic Medicine School, Sichuan University, Chengdu 610041, China. wangyh11@163.com
Liu
Min
M
Peng
Xue-Mei
XM
Li
Fan
F
Zhang
Fu
F
Zhang
Kui
K
Liao
Zhi-Gang
ZG
D000328:Adult; D001772:Blood Cell Count; D002462:Cell Membrane / Q000648:ultrastructure*; D002463:Cell Membrane Permeability; D018274:Electroporation / Q000379:methods*; D004912:Erythrocytes / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000648:ultrastructure*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D055815:Young Adult