title: Preliminary characterization of (nucleoside-2'-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses.
abstract: Viral methyltranferases (MTase) are involved in the third step of the mRNA-capping process, transferring a methyl group from S-adenosyl-L-methionine (SAM) to the capped mRNA. MTases are classified into two groups: (guanine-N7)-methyltransferases (N7MTases), which add a methyl group onto the N7 atom of guanine, and (nucleoside-2'-O-)-methyltransferases (2'OMTases), which add a methyl group to a ribose hydroxyl. The MTases of two flaviviruses, Meaban and Yokose viruses, have been overexpressed, purified and crystallized in complex with SAM. Characterization of the crystals together with details of preliminary X-ray diffraction data collection (at 2.8 and 2.7 angstroms resolution, respectively) are reported here. The sequence homology relative to Dengue virus 2'OMTase and the structural conservation of specific residues in the putative active sites suggest that both enzymes belong to the 2'OMTase subgroup.
authors.affiliation: Department of Biomolecular Sciences and Biotechnology, CNR-INFM, University of Milano, Via Celoria 26, 20133 Milano, Italy.
authors.forename: Eloise
authors.initials: E
authors.lastname: Mastrangelo
authors.forename: Michela
authors.initials: M
authors.lastname: Bollati
authors.forename: Mario
authors.initials: M
authors.lastname: Milani
authors.forename: Xavier
authors.initials: X
authors.lastname: de Lamballerie
authors.forename: Xavier
authors.initials: X
authors.lastname: de Lamballeire
authors.forename: Nadège
authors.initials: N
authors.lastname: Brisbarre
authors.forename: Nadege
authors.initials: N
authors.lastname: Brisbare
authors.forename: Karen
authors.initials: K
authors.lastname: Dalle
authors.forename: Violaine
authors.initials: V
authors.lastname: Lantez
authors.forename: Marie Pierre
authors.initials: MP
authors.lastname: Egloff
authors.forename: Bruno
authors.initials: B
authors.lastname: Coutard
authors.forename: Bruno
authors.initials: B
authors.lastname: Canard
authors.forename: Ernest
authors.initials: E
authors.lastname: Gould
authors.forename: Naomi
authors.initials: N
authors.lastname: Forrester
authors.forename: Martino
authors.initials: M
authors.lastname: Bolognesi
mesh_terms: D003460:Crystallization; D005416:Flavivirus / Q000201:enzymology*; D008780:Methyltransferases / Q000737:chemistry* / Q000302:isolation & purification; D014764:Viral Proteins / Q000737:chemistry / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D014764:Viral Proteins; D008780:Methyltransferases; C055099:mRNA (nucleoside-O(2'))-methyltransferase