Inhibition of PRL-3 gene expression in gastric cancer cell line SGC7901 via microRNA suppressed reduces peritoneal metastasis.
High expression of PRL-3, a protein tyrosine phosphatase, is proved to be associated with lymph node metastasis in gastric carcinoma from previous studies. In this paper, we examined the relationship between PRL-3 expression and peritoneal metastasis in gastric carcinoma. We applied the artificial miRNA (pCMV-PRL3miRNA), which is based on the murine miR-155 sequence, to efficiently silence the target gene expression of PRL-3 in SGC7901 gastric cancer cells at both mRNA and protein levels. Then we observed that, in vitro, pCMV-PRL3miRNA significantly depressed the SGC7901 cell invasion and migration independent of cellular proliferation. In vivo, PRL-3 knockdown effectively suppressed the growth of peritoneal metastases and improved the prognosis in nude mice. Therefore, we concluded that artificial miRNA can depress the expression of PRL-3, and that PRL-3 might be a potential therapeutic target for gastric cancer peritoneal metastasis.
Li
Zhengrong
Z
Department of Gastrointestinopancreatic Surgery, First Affiliated Hospital, Sun Yat-sen University, Gastric Center of Sun Yat-sen University, Guangzhou 510080, China.
Zhan
Wenhua
W
Wang
Zhao
Z
Zhu
Baohe
B
He
Yulong
Y
Peng
Junsheng
J
Cai
Shirong
S
Ma
Jinping
J
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs; D009368:Neoplasm Transplantation; D010534:Peritoneal Neoplasms / Q000235:genetics / Q000556:secondary; D010957:Plasmids; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D017874:Immediate-Early Proteins; D035683:MicroRNAs; C111534:Ptp4a3 protein, mouse; D017027:Protein Tyrosine Phosphatases