title: A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis.
abstract: Study of the complexome - all the protein complexes of the cell - is essential for a better understanding and more global vision of cell function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic and membrane protein complexes of Escherichia coli were separated. Then, the different partners of each protein complex were identified by LC-MS/MS. In this report, 306 protein complexes were separated and identified. Among these protein complexes, 50 heteromultimeric and 256 homomultimeric protein complexes were found. Among the 50 heteromultimeric protein complexes, 18 previously described protein complexes validate the technology. In this study, 109 new protein complexes were found, providing insight into the function of previously uncharacterized bacterial proteins.
authors.affiliation: Plateforme Génomique Fonctionnelle Bordeaux - Pôle Protéomique, Université Victor Segalen Bordeaux 2, Bordeaux, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Lasserre
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Beyne
authors.forename: Slovénie
authors.initials: S
authors.lastname: Pyndiah
authors.forename: Delphine
authors.initials: D
authors.lastname: Lapaillerie
authors.forename: Stéphane
authors.initials: S
authors.lastname: Claverol
authors.forename: Marc
authors.initials: M
authors.lastname: Bonneu
mesh_terms: D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis* / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D020543:Proteome