title: Fully automated gradient elution liquid chromatographic assay of omeprazole and two metabolites.
abstract: An automated liquid chromatographic method for the determination of omeprazole and two metabolites in plasma and urine is described. It utilizes the Technicon Fully-Automated-Sample-Treatment-LC system (FAST(R)-LC). Sample preparation is achieved by air-segmented continuous-flow providing solvent extraction, evaporation to dryness and reconstitution before injection onto a reversed-phase column. The compounds are separated by isocratic or gradient elution with acetonitrile-phosphate buffer mobile phases and quantified by UV-measurements at 302 nm. The limit of determination (relative standard deviation 10-15%) is 50 nmol l(-1) in plasma (800 microl) and 200 nmol l(-1) in urine (200 microl). The sample capacity is six or three samples per hour, depending on the elution mode.
authors.affiliation: Bioanalytical Chemistry, AB Hässle, S-431 83 Mölndal, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Grundevik
authors.forename: G
authors.initials: G
authors.lastname: Jerndal
authors.forename: K
authors.initials: K
authors.lastname: Balmér
authors.forename: B A
authors.initials: BA
authors.lastname: Persson