title: An estrogen-platinum terpyridine conjugate: DNA and protein binding and cellular delivery.
abstract: A platinum metal complex in which terpyridine joins estradiol (via an ethynyl link) to a platinum with a labile ligand (chloride) has been designed, synthesised and its X-ray crystal structure determined. The aim of this work was to link a targeting motif (in this case estrogen) to a metal-based biomolecule recognition unit (the platinum moiety). The target molecule: 17alpha-[4'-ethynyl-2,2':6',2'-terpyridine]-17beta-estradiol platinum(II) chloride (PtEEtpy) has been shown to bind to both human and bovine serum albumin (SA) and to DNA. FTICR mass spectrometry shows that the bimolecular units are in each case linked through coordination to the platinum with displacement of the chloride ligand. Circular dichroism indicates that a termolecular entity involving PtEEtpy, SA and DNA is formed. A range of electrospray mass spectrometry experiments showed that the PtEEtpy complex breaks and forms coordination bonds relatively easily. A whole cell estrogen receptor assay in an estrogen receptor positive cell (MCF-7) confirms binding of both EEtpy and PtEEtpy to the estrogen receptor in cells. The work demonstrates the concept of linking a targeting moiety (in this case estrogen) to a DNA binding agent.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK. m.j.hannon@bham.ac.uk
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hannon
authors.forename: Philip S
authors.initials: PS
authors.lastname: Green
authors.forename: Dianne M
authors.initials: DM
authors.lastname: Fisher
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Derrick
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Beck
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Watt
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Ralph
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Sheil
authors.forename: Philip R
authors.initials: PR
authors.lastname: Barker
authors.forename: Nathaniel W
authors.initials: NW
authors.lastname: Alcock
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Price
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Sanders
authors.forename: Richard
authors.initials: R
authors.lastname: Pither
authors.forename: Julie
authors.initials: J
authors.lastname: Davis
authors.forename: Alison
authors.initials: A
authors.lastname: Rodger
mesh_terms: D000818:Animals; D001483:Base Sequence; D001681:Biological Assay; D002417:Cattle; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D006801:Humans; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D012709:Serum Albumin / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: C521296:17alpha-(4'-ethynyl-2,2':6',2'-terpyridine)-17beta-estradiol platinum(II) chloride; D009944:Organoplatinum Compounds; D011960:Receptors, Estrogen; D012709:Serum Albumin; D004247:DNA