title: [Measurement of affinity constant of monoclonal antibody by competitive antibody binding to antigen].
abstract: OBJECTIVE
  To develop a more reliable and stable method for determining monoclonal antibody (mAb) affinity constant based on competitive antibody/antigen binding.
  
  
  METHODS
  The Kd value was calculated based on the relationship between the binding proportion of the antigen and the original concentration of the mAb that competed for the binding site of the antigen.
  
  
  RESULTS
  The Kd values measured with this improved method under two different conditions were 2.61x10(-12) mol/L and 2.39x10(-12) mol/L, and those with Friguent method in these two conditions were 5.57x10(-10) mol/L and 1.41x10(-10) mol/L, respectively.
  
  
  CONCLUSIONS
  Compared with Friguent method, the Kd values measured with this improved method are closer to the actual value, and the measurement results under different experiment conditions are more stable.
authors.affiliation: Zodolabs Biotechnology Limited Company, Nanchang 330029, China. Efjzlq@tom.com
authors.forename: Jie-biao
authors.initials: JB
authors.lastname: Guo
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Liu
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex