title: Capillary electrophoretic determination of thiamphenicol in turkeys serum and its pharmacokinetic application.
abstract: The pharmacokinetics of thiamphenicol were investigated in 12 healthy turkeys of both sexes, following intravenous (i.v.) and intragastric (p.o.) administration of a single dose of 30 mg/kg body weight (bw). Serum drug concentrations were determined by capillary electrophoresis technique on blood samples collected over 24 h following treatment. The method was statistically validated for its linearity, accuracy, precision and selectivity. The linear range was from 0.2 to 500 microg/ml with correlation coefficients greater than 0.999. The limit of detection of drug was 70 ng/ml, while the quantitative limit was 200 ng/ml, using 0.5 ml sample size. Pharmacokinetic variables of the drug were calculated after both administration routes.
authors.affiliation: Medical University of Gdańsk, Faculty of Pharmacy, Hallera 107, PL-80-416 Gdańsk, Poland. piotrpl@wp.pl
authors.forename: P
authors.initials: P
authors.lastname: Kowalski
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D019540:Area Under Curve; D001682:Biological Availability; D019075:Electrophoresis, Capillary; D005260:Female; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D007441:Intubation, Gastrointestinal; D008297:Male; D012015:Reference Standards; D015203:Reproducibility of Results; D013839:Thiamphenicol / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D014422:Turkeys / Q000097:blood*
chemical_list: D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D013839:Thiamphenicol