Haem oxygenase-1 expression and coronary heart disease--association between levels of haem oxygenase-1 expression and angiographic morphology as well as the quantity of coronary lesions.
OBJECTIVE
The aim of this paper was to investigate the association between levels of HO-1 expression and angiographic morphology as well as the quantity of coronary lesions in patients with coronary heart disease (CHD).


METHODS
110 patients with CHD were diagnosed by coronary angiography which contained coronary lesions in some way. Firstly, the patients were divided into 3 groups according to the angiographic morphology of their coronary lesions: type I (smooth borders) group (n1= 36), type II (irregular borders) group (n2= 48) and type III (long and irregular lesions) group (n3= 26). Secondly, the patients were split into a further 3 groups, named: single-vessel group (SV, 38 cases), double-vessel group (DV, 44 cases) and multi-vessel group (MV, 28 cases) according to the number of coronary lesions. Another 30 patients with normal coronary arteries (diagnosed by coronary angiography) were selected as the control group. The levels of HO-1 protein expression in monocytes and lymphocytes from the subjects were tested by immunohistochemistry and Western blot. A computer picture analysing system was also used to measure the levels of HO-1 protein expression.


RESULTS
HO-1 protein was located in cell plasma and the levels of HO-1 protein expression in patients with CHD were significantly higher than in those without CHD (p < 0.01). There were significant differences of HO-1 expression among patients with CHD. Patients with type III lesions had the highest levels, followed by those with type II lesions and the lowest levels were found in patients with type I lesions (p < 0.01). Also, levels of HO-1 protein expression in patients with multi-vessel disease and double-vessel disease were significantly higher than in those with single-vessel disease (p < 0.01).


CONCLUSIONS
There is a higher expression of HO-1 in patients with CHD and the levels of HO-1 protein are associated with severity of CHD angiographically.
Li
Yu-Guang
YG
Department of Cardiology, The First Affiliated Hospital, Medical College, Shantou University, Shantou Guangdong, China.
Wang
Dong-Ming
DM
Chen
Song-Ming
SM
Tan
Xue-Rui
XR
Fang
Xiao-Yi
XY
Wu
Jie-Wen
JW
Zhang
Guo-Hong
GH
Mai
Rui-Qing
RQ
D000368:Aged; D015153:Blotting, Western; D017023:Coronary Angiography; D003327:Coronary Disease / Q000000981:diagnostic imaging / Q000201:enzymology* / Q000473:pathology; D003331:Coronary Vessels / Q000201:enzymology / Q000473:pathology; D003593:Cytoplasm / Q000201:enzymology / Q000473:pathology; D005260:Female; D006419:Heme Oxygenase (Decyclizing) / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000201:enzymology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology / Q000473:pathology; D011379:Prognosis
D006419:Heme Oxygenase (Decyclizing)