Rhus verniciflua stokes extract: radical scavenging activities and protective effects on H2O2-induced cytotoxicity in macrophage RAW 264.7 cell lines.
The goal of this study was to determine the effects of Rhus verniciflua stokes (RVS) on the radical scavenging activities and H2O2-induced cytotoxicity in macrophage 264.7 cells. An 80% ethanol extract of RVS was purified by ion exchange chromatography (IEC). Purified RVS extract contained a high amount of phenolics (55.5 g/100 g of extract) and flavonoids (5.9 g/100 g of extract) and its antioxidant activities, such as DPPH, superoxide anion and hydroxyl radical scavenging activities, was higher than the unpurified RVS extract. Purified RVS extract significantly reduced intracellular ROS formation caused by H2O2. Purified RVS extract also prevented the cell death of macrophage RAW 264.7 cells induced by H2O2. Interestingly, co-treated purified RVS extract effectively reduced thiobarbituric acid reactive substance (TBARS) formation, and attenuated catalase depletion at concentration of 100 microg/ml (p<0.05). Study using PDA-HPLC analyses have found that the purified RVS extract contains six major low molecular compounds as p-coumaric acid, fustin, kaempferol-3-O-glucoside, sulfuretin, butein and kaempferol. These results indicate that purified RVS extract, tested in this study, could contribute to the antioxidant activities and inhibition of intracellular ROS level.
Jung
Chang Hwa
CH
Laboratory of Clinical Biology and Pharmacogenomics, College of Oriental Medicine, Kyunghee University, South Korea.
Jun
Chan-Yong
CY
Lee
Sundong
S
Park
Chong-Hyeong
CH
Cho
Kiho
K
Ko
Seong-Gyu
SG
D000704:Analysis of Variance; D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D029045:Rhus / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D016166:Free Radical Scavengers; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D006861:Hydrogen Peroxide