title: An efficient genetic screen in Drosophila to identify nuclear-encoded genes with mitochondrial function.
abstract: We conducted a screen for glossy-eye flies that fail to incorporate BrdU in the third larval instar eye disc but exhibit normal neuronal differentiation and isolated 23 complementation groups of mutants. These same phenotypes were previously seen in mutants for cytochrome c oxidase subunit Va. We have molecularly characterized six complementation groups and, surprisingly, each encodes a mitochondrial protein. Therefore, we believe our screen to be an efficient method for identifying genes with mitochondrial function.
authors.affiliation: Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.
authors.forename: T S Vivian
authors.initials: TS
authors.lastname: Liao
authors.forename: Gerald B
authors.initials: GB
authors.lastname: Call
authors.forename: Preeta
authors.initials: P
authors.lastname: Guptan
authors.forename: Albert
authors.initials: A
authors.lastname: Cespedes
authors.forename: Jamie
authors.initials: J
authors.lastname: Marshall
authors.forename: Kevin
authors.initials: K
authors.lastname: Yackle
authors.forename: Edward
authors.initials: E
authors.lastname: Owusu-Ansah
authors.forename: Sudip
authors.initials: S
authors.lastname: Mandal
authors.forename: Q Angela
authors.initials: QA
authors.lastname: Fang
authors.forename: Gelsey L
authors.initials: GL
authors.lastname: Goodstein
authors.forename: William
authors.initials: W
authors.lastname: Kim
authors.forename: Utpal
authors.initials: U
authors.lastname: Banerjee
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics; D000818:Animals; D001126:Arginine-tRNA Ligase / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods; D003433:Crosses, Genetic; D004330:Drosophila / Q000235:genetics*; D004625:Embryo, Nonmammalian; D005123:Eye / Q000196:embryology / Q000254:growth & development; D005260:Female; D005820:Genetic Testing / Q000379:methods*; D019476:Insect Proteins / Q000235:genetics*; D008190:Lyases / Q000235:genetics; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000096:biosynthesis* / Q000235:genetics; D008954:Models, Biological; D009154:Mutation; D019884:Nitrogenous Group Transferases / Q000235:genetics
chemical_list: D019476:Insect Proteins; D024101:Mitochondrial Proteins; D019883:Alkyl and Aryl Transferases; C021386:4-hydroxybenzoate polyprenyltransferase; D019884:Nitrogenous Group Transferases; C047694:glutamyl-tRNA(Gln) amidotransferase; D008190:Lyases; C031581:cytochrome C synthetase; D001126:Arginine-tRNA Ligase