[Aquaporin and Na+ transport of alveolar type II cells in rats with oleic acid-induced acute respiratory distress syndrome].
OBJECTIVE
To evaluate the capacity of active water and sodium transport of alveolar type II (AT II cells in rats with acute respiratory distress syndrome (ARDS).


METHODS
AT II cells were isolated and purified from rats with ARDS, and the distribution of aquaporin-1 (AQP-1) on the cells was observed by immunocytochemistry, immunoelectron microscopy and Western blotting. The sodium currents were detected by patch-clamp technique in whole-cell recording mode.


RESULTS
Immunocytochemistry showed staining for AQP-1 in rat AT II cells, and immunoelectron microscope demonstrated the presence of AQP-1, which was decreased as shown by Western blotting in comparison with the control group. The whole-cell currents were also decreased, but remained sensitive to terbutalin treatment.


CONCLUSIONS
The capacity of active water and sodium transport is damaged, but not totally lost in AT II cells of ARDS rats. Terbutalin can increase the currents even if in pathological conditions. AQP-1 and sodium transport of AT II cells play an important role in pulmonary liquid clearance.
Li
Tao-ping
TP
Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. ltp@fimmu.com
Shen
Hai-yan
HY
Liu
Lin
L
D000208:Acute Disease; D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism*; D015153:Blotting, Western; D027682:Cation Transport Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D017136:Ion Transport; D008171:Lung Diseases / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D016253:Microscopy, Immunoelectron; D019301:Oleic Acid; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*; D013577:Syndrome
C496020:AQP1 protein, human; D027682:Cation Transport Proteins; D051398:Aquaporin 1; D019301:Oleic Acid; D012964:Sodium