Blood compatibility of novel water soluble hyperbranched polyglycerol-based multivalent cationic polymers and their interaction with DNA.
A novel class of hyperbranched polymers based on polyglycerol (PG) and poly(ethylene glycol) (PEG) are synthesized by multibranching anionic ring opening polymerization. Multivalent cationic sites are added to these polymers by a post-amination and quarternization reactions. Blood compatibility studies using these polymers at different concentrations showed insignificant effects on complement activation, platelet activation, coagulation, erythrocyte aggregation and hemolysis compared to branched cationic polyethyleneimine (PEI). The degree of quarternization does not have large influence on the blood compatibility of the new polymers. Cytotoxicity of these polymers is significantly lower than that of PEI and is a function of quarternized nitrogen present in the polymer. Also, these polymers bind DNA in the nanomolar range and are able to condense DNA to highly compact, stable, water soluble nanoparticles in the range of 60-80 nm. Gel electrophoresis studies showed that they form electroneutral complexes with DNA around N/P ratio 1 irrespective of the percentage of quarternization under the conditions studied.
Kainthan
Rajesh Kumar
RK
Centre for Blood Research and Department of Pathology and Lab Medicine, 2350 Health Sciences Mall, Life Science Centre, University of British Columbia, Vancouver, BC, Canada V6T 1Z3.
Gnanamani
Muthiah
M
Ganguli
Munia
M
Ghosh
Tanay
T
Brooks
Donald E
DE
Maiti
Souvik
S
Kizhakkedathu
Jayachandran N
JN
D001672:Biocompatible Materials; D001769:Blood; D002413:Cations, Divalent; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D015539:Platelet Activation; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry*
D001672:Biocompatible Materials; D002413:Cations, Divalent; D011108:Polymers; D014867:Water; C043941:polyglycerol; D011092:Polyethylene Glycols; D004247:DNA; D005990:Glycerol