title: The pathogenesis of Newcastle disease: a comparison of selected Newcastle disease virus wild-type strains and their infectious clones.
abstract: The effect of mutations of Newcastle disease virus (NDV) fusion (F) gene, hemagglutinin-neuraminidase (HN) gene, and phosphoprotein (P) gene and HN chimeras between the virulent Beaudette C and low virulence LaSota strains on pathogenesis and pathogenicity was examined in fully susceptible chickens. A virulent F cleavage site motif within a LaSota backbone increased pathogenicity and severity of clinical disease. A LaSota HN within a Beaudette C backbone decreased pathogenicity indices and disease severity. A Beaudette C HN within a LaSota backbone did not change either pathogenicity indices or severity of disease in chickens. Loss of glycosylation at site 4 of the HN or modified P gene of Beaudette C decreased pathogenicity indices and caused no overt clinicopathologic disease in chickens. Both pathogenicity indices and clinicopathologic examination demonstrated that the F, HN, and P genes of NDV collectively or individually can contribute to viral virulence.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, GA 30605, USA. wakamatsun@niehs.nih.gov
authors.forename: Nobuko
authors.initials: N
authors.lastname: Wakamatsu
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: King
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Seal
authors.forename: Siba K
authors.initials: SK
authors.lastname: Samal
authors.forename: Corrie C
authors.initials: CC
authors.lastname: Brown
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D015707:HN Protein / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics / Q000472:pathogenicity*; D010750:Phosphoproteins / Q000235:genetics; D014760:Viral Fusion Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence
chemical_list: D015707:HN Protein; C055691:P phosphoprotein, Newcastle disease virus; D010750:Phosphoproteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins