Determination of antibiotics and antimicrobial agents in human serum by direct injection onto silica liquid chromatographic columns.
Antibiotics and antimicrobial agents are therapeutically monitored by the direct injection of untreated human serum onto silica columns. This simple approach does not lead to rapid column deterioration since the eluents utilized are almost totally aqueous and, consequently, prevent precipitation of the serum protein on the column. Column capacity of the drugs studied varies due to the mutual interaction of the eluent ionic buffer and organic modifiers. The influence of the number of serum injections on column-back pressure, column capacity and quantitative analyses is discussed.
Adamovics
J A
JA
Squibb Institute for Medical Research, New Brunswick, New Jersey 08903, USA.