[Determination of artemisinin in Artemisia annuna by HPLC-ELSD].
OBJECTIVE
A HPLC method was first established to determine artemisinin in Artemisia annuna by HPLC-ELSD. Artemisinin in Herba of Artemisia annuna from different places was determined by this new method.


METHODS
The method utilized MeOH-H2O (75: 25) as mobile phase with flow rate of 1 ml/min, C18 (250 x 4.6 mm, 5 microm) column. The temperature of detector was 40 degrees C, the pressure of N2 was 3.5 bar, and gain value was 9.


RESULTS
The calibration curves were linear within the range of 1 - 5 microg, and the average recovery was 99.33% (RSD = 1.97%).


CONCLUSIONS
The method was rapid, convenient, and accurate. It can be used for the quality control of this herbal medicine.
Zhou
Ao-Ao
AA
National Engineering Research Center for Modernization of Extraction and Separation Process of TCM, Guangzhou 510240, China.
Zheng
Wen-Xin
WX
Ge
Fa-Huan
FH
D000962:Antimalarials / Q000737:chemistry; D031172:Artemisia annua / Q000737:chemistry* / Q000145:classification; D037621:Artemisinins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012621:Seasons; D012717:Sesquiterpenes / Q000032:analysis*
D000962:Antimalarials; D037621:Artemisinins; D004365:Drugs, Chinese Herbal; D012717:Sesquiterpenes; C031327:artemisinin