title: [Search for active psychrotrophic microbial oil degraders and their characterization].
abstract: The ability of 96 microbial strains degrading oil and 32 strains degrading polycyclic aromatic hydrocarbons (PAHs) to consume diesel fuel and oil at 4-6 degrees C and 24 degrees C and at elevated NaCl concentrations was studied. The temperature range, salt tolerance, ability to produce bioemulsifiers, range of substrates, and antibiotic resistance were determined. The eleven most active oil-degrading and PAH-degrading strains were genotyped by a polymerase chain reaction with BoxA1R primers and a restriction analysis of ribosomal DNA amplicons. For six strains, the degree of oil degradation at 4-6 degrees C was higher than at 24 degrees C. For the most active strains, the degree of oil degradation in liquid mineral medium ranged from 15 to 26% at 24 degrees C and from 28 to 47% at 4-6 degrees C. An artificial association of six of the strains degraded the oil by 46% at 24 degrees C.
authors.forename: I A
authors.initials: IA
authors.lastname: Pyrchenkova
authors.forename: A B
authors.initials: AB
authors.lastname: Gafarov
authors.forename: I F
authors.initials: IF
authors.lastname: Puntus
authors.forename: A E
authors.initials: AE
authors.lastname: Filonov
authors.forename: A M
authors.initials: AM
authors.lastname: Boronin
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D001673:Biodegradation, Environmental; D003080:Cold Temperature; D005742:Gasoline / Q000382:microbiology*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D005742:Gasoline; D012989:Soil Pollutants