Emulsions of oil from Adenanthera pavonina L. seeds and their protective effect.
In our previous study, we developed very stable formulations of submicron oil-in-water emulsions from Adenanthera pavonina L. (family Leguminosae, subfamily Mimosoideae) seed oil, stabilised with soybean lecithin (SPC). Continuing our research, we introduced an additional co-emulsifier, Tween 80, to those formulations in order to decrease the size of the emulsion particles and improve their stability. Formulations with a mean particle size ranging from 43.6 to 306.5 nm and a negative surface charge from -45.3 to -28.5 mV were obtained. Our stability experiments also revealed that most of the tested formulations had a very good degree of stability over a 3-month storage period, both at 4 degrees C and at room temperature. Since many intravenous injectable drugs exhibit lytic activity against erythrocytes, we examined this activity for the emulsion form of cardol, a natural compound with already proven hemolytic properties. The incorporation of this agent into the emulsion caused an evident decrease in hemolytic activity (97-99%). This highly protective effect, observed against sheep erythrocytes, was independent of both the composition and the particle size of the emulsions used. Our studies suggest that nonionic surfactant/phospholipid-based emulsions containing this edible oil of A. pavonina L. may be useful as an alternative formulation matrix for pharmaceutical, nutritional or cosmetic applications of otherwise membrane-acting components.
Jaromin
Anna
A
Department of Lipids and Liposomes, Institute of Biochemistry and Molecular Biology, University of Wrocław, Przybyszewskiego 63/77, Wrocław, Poland. ajaromin@ibmb.uni.wroc.pl
Zarnowski
Robert
R
Kozubek
Arkadiusz
A
D000818:Animals; D004655:Emulsions; D004912:Erythrocytes / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects*; D010316:Particle Size; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D012756:Sheep; D013696:Temperature
D004655:Emulsions; D010938:Plant Oils; D020011:Protective Agents