[Antioxidation activities of Fructus Broussonetia haematochrome in vitro].
OBJECTIVE
To investigate the antioxidative effects of Fructus Broussonetia Haematochrome.


METHODS
NADH-PMS-NBT system was used to produce supetoxide free radical (O2-*), Fe(2+) -H2O2 system to generate hydroxyl free radical (*OH), H2O2 to stimulate oxidative hemolysis of erythrocytes of mice. MDA production in liver homogenate induced by auto-oxidation was measured by MDA kits method.


RESULTS
FBH not only scavenged O2-* and *OH produced by the experimental systems directly, but also inhibited H2O2 stimulated oxidative hemolysis of erythrocytes of mice, depressed MDA production in mice liver homogenate by auto-oxidation and hepatic mitochondria expanded induced by Vit C-Fe2+ system.


CONCLUSIONS
FBH possess an antioxidative activity.
Pang
Su-Qiu
SQ
College of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Wang
Guo-Quan
GQ
Qin
Lu-Ping
LP
Huang
Bao-Kang
BK
Zhang
Qiao-Yan
QY
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects*; D017665:Hydroxyl Radical / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D029586:Moraceae / Q000737:chemistry*; D010860:Pigments, Biological / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry
D000975:Antioxidants; D016166:Free Radical Scavengers; D010860:Pigments, Biological; D017665:Hydroxyl Radical; D008315:Malondialdehyde