title: Visfatin mRNA expression in human subcutaneous adipose tissue is regulated by exercise.
abstract: Visfatin [pre-beta-cell colony-enhancing factor (PBEF)] is a novel adipokine that is produced by adipose tissue, skeletal muscle, and liver and has insulin-mimetic actions. Regular exercise enhances insulin sensitivity. In the present study, we therefore examined visfatin mRNA expression in abdominal subcutaneous adipose tissue and skeletal muscle biopsies obtained from healthy young men at time points 0, 3, 4.5, 6, 9, and 24 h in relation to either 3 h of ergometer cycle exercise at 60% of Vo(2 max) or rest. Adipose tissue visfatin mRNA expression increased threefold at the time points 3, 4.5, and 6 h in response to exercise (n = 8) compared with preexercise samples and compared with the resting control group (n = 7, P = 0.001). Visfatin mRNA expression in skeletal muscle was not influenced by exercise. The exercise-induced increase in adipose tissue visfatin was, however, not accompanied by elevated levels of plasma visfatin. Recombinant human IL-6 infusion to mimic the exercise-induced IL-6 response (n = 6) had no effect on visfatin mRNA expression in adipose tissue compared with the effect of placebo infusion (n = 6). The finding that exercise enhances subcutaneous adipose tissue visfatin mRNA expression suggests that visfatin has a local metabolic role in the recovery period following exercise.
authors.affiliation: Centre of Inflammation and Metabolism, Rigshospitalet Section 7641, Blegdamsvej 9, DK-2100, Copenhagen, Denmark.
authors.forename: Lone
authors.initials: L
authors.lastname: Frydelund-Larsen
authors.forename: Thorbjorn
authors.initials: T
authors.lastname: Akerstrom
authors.forename: Søren
authors.initials: S
authors.lastname: Nielsen
authors.forename: Pernille
authors.initials: P
authors.lastname: Keller
authors.forename: Charlotte
authors.initials: C
authors.lastname: Keller
authors.forename: Bente Klarlund
authors.initials: BK
authors.lastname: Pedersen
mesh_terms: D050153:Abdominal Fat / Q000378:metabolism*; D000328:Adult; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D015444:Exercise / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D054409:Nicotinamide Phosphoribosyltransferase; D010919:Placebos; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D050151:Subcutaneous Fat / Q000378:metabolism*
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D010919:Placebos; D012333:RNA, Messenger; D011994:Recombinant Proteins; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human