[Study on tissue culture and rapid propagation of Atractylodes macrocephala].
A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz.. The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation. MS + BA 1.0 mg/L + NAA 0.3 mg/L + GA3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also. MS + BA 3.0 mg/L + IBA 0.5 mg/L was suitable for the proliferation of axillary buds, with an induction rate of 95%. 1/2MS + IBA 0.1 - 0.5 mg/L was optimum for rooting, with a rooting percentage more than 90%. The survival rate of transplanted plantlets was more than 90%.
Zhu
Yu-Qiu
YQ
Faculty of Forestry and Biotechnology, Zhejiang Forestry University, Lin'an, Zhejiang 311300, China.
Xia
Guo-Hua
GH
Fang
Hui-Gang
HG
Fu
Shun-Hua
SH
He
Fu-Ji
FJ
D031174:Atractylodes / Q000254:growth & development*; D003470:Culture Media; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators