Contamination with PCR detectable virus in a virus isolation quality assurance panel.
External quality control schemes are an essential part of the quality assurance of all diagnostic virology laboratories. There are a few providers of nucleic acid detection quality control panels. Consequently, diagnostic laboratories may test panels that are developed specifically for virus isolation by nucleic acid detection methods. Here, we report on a recent simulated eye swab panel from a National external quality assessment service, which was meant for virus isolation but which we tested by polymerase chain reactions assays. The results suggest that the samples had been contaminated at source leading to difficulty in interpretation of the panel and a poor score.
Gunson
R N
RN
West of Scotland Specialist Virology Centre, Gartnavel General Hospital, Glasgow G12 0YN, United Kingdom. Rory.Gunson@NorthGlasgow.Scot.NHS.UK
Abraham
E
E
Carman
W F
WF
D005189:False Positive Reactions; D016133:Polymerase Chain Reaction; D011786:Quality Control; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D014773:Virology / Q000379:methods*; D014780:Viruses / Q000302:isolation & purification*
D011933:Reagent Kits, Diagnostic