[The study of salivary S-IgA antibody activity to clinical Streptococcus mutans in heat treated stress].
OBJECTIVE
The purpose of this study was to compare the salivary immunoglobulin A antibody response to Streptococcus mutans in normal with in heat treated stress.


METHODS
Clinical Streptococcus mutans strains were isolated from 20 volunteers, serotyped by biochemical test and PCR, and genotyped by AP-PCR. Unstimulated secretions from submandibular glands and sublingual glands were collected from volunteers by modified collectors. Each identified genotype was cultured in two groups: control group was grown in BHI broth at 37 degrees C. for 3 hours; stress group was incubated in BHI broth at 42 degrees C. for 3 hours. Analysis of SIgA activity to clinical genotype strains and reference strains in different group was detected by Western blot.


RESULTS
There was no significant difference between stress group and control group,in spite that some bands had strong or weak intensity. Different genotypes of S.mutans could have different immunoblotting profile as for an individual. SIgA from different volunteers could have different immonoblotting profiles as to the same genotype strain.


CONCLUSIONS
Although Streptococcus mutans can express heat shock proteins in stress, this study suggests these new proteins have no significant effect on the reaction of SIgA to Streptococcus mutans. Different genotype strains may have different proteins, and different immunoreactivity to host. Different hosts may have different immunoreactivities to one genotypes of S.mutans.
Nie
Min
M
Department of Endodontics, School and Hospital of Stomatology, Wuhan 430079, Hubei Province, China. nm.minmin@tom.com
Bian
Zhuan
Z
Fan
Ming-wen
MW
Hu
Ping
P
Meng
Liu-yan
LY
Liu
Jia-rong
JR
D015153:Blotting, Western; D018869:Heat-Shock Response; D006358:Hot Temperature; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D012463:Saliva / Q000276:immunology*; D013295:Streptococcus mutans / Q000276:immunology*
D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory