[In vitro and in vivo investigations on the treatment of presbyopia using femtosecond lasers].
BACKGROUND
Ultrashort (femtosecond) laser pulses can generate precise cuts in biological tissue without damaging the surface. The application of femtosecond laser technology at the lens was evaluated with respect to a possible treatment of presbyopia.


METHODS
Femtosecond laser lentotomy was performed on 150 pig lenses in vitro. Cutting geometry and laser settings were optimized to generate smooth cuts with a minimum of produced gas bubbles. Four rabbit lenses were treated afterwards in vivo and were controlled for 3 months post-treatment. The lenses were then extracted and evaluated.


RESULTS
With suitable laser settings, light scattering due to residual gas bubbles could be almost completely avoided in pig lenses. A pulse energy of less than 1.2 microJ and a cutting geometry with spot separations of more than 5 microm are important. The rabbit lenses stayed macroscopically clear for 3 months in vivo. Only the cell structures directly adjacent to the laser focus were cut; structures 5-10 microm away appeared to be intact. No cataract formation occurred during this time.


CONCLUSIONS
Femtosecond laser application allows precise and smooth cuts inside pig and rabbit lenses without damage to adjacent tissue.
Gerten
G
G
Laserforum Köln e.V., Augenklinik am Neumarkt, Schildergasse 107, 109, 50667, Köln, Germany. info@augenportal.de
Ripken
T
T
Breitenfeld
P
P
Krueger
R R
RR
Kermani
O
O
Lubatschowski
H
H
Oberheide
U
U
D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053685:Laser Therapy / Q000295:instrumentation* / Q000379:methods; D007908:Lens, Crystalline / Q000473:pathology* / Q000601:surgery*; D011305:Presbyopia / Q000473:pathology* / Q000601:surgery*; D013552:Swine; D016896:Treatment Outcome