title: Cryptosporidium, Giardia and Enterocytozoon bieneusi in cats from Bogota (Colombia) and genotyping of isolates.
abstract: The prevalence of Cryptosporidium, Giardia, and Enterocytozoon bieneusi in cats from Bogota (Colombia) was determined from fecal specimens and scrapings of duodenal and ileal mucosa screened by PCR. All PCR-positive specimens were sequenced to determine the genotype(s) present. Of 46 cats, 6 (13%) were positive for Cryptosporidium, 5 (11%) were infected with C. felis and one (2%) with C. muris. Three (6.5%) cats were infected with Giardia duodenalis Assemblage F. Eight (17%) cats were infected with four genotypes of E. bieneusi: genotype D-like (9%), K (4%), Peru 10 (2%), and Peru 5 (2%). This is the first report on the presence of zoonotic species/genotypes of Cryptosporidium and E. bieneusi in cats in Colombia.
authors.affiliation: Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.
authors.forename: Mónica
authors.initials: M
authors.lastname: Santín
authors.forename: James M
authors.initials: JM
authors.lastname: Trout
authors.forename: Jesús A Cortés
authors.initials: JA
authors.lastname: Vecino
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: Ronald
authors.initials: R
authors.lastname: Fayer
mesh_terms: D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000453:epidemiology*; D002415:Cats; D003105:Colombia; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000662:veterinary*; D003458:Cryptosporidium / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry*; D016054:DNA, Protozoan / Q000737:chemistry*; D021862:Enterocytozoon / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology / Q000469:parasitology; D005260:Female; D005784:Gene Amplification; D005838:Genotype; D005872:Giardia / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000662:veterinary*; D008297:Male; D016881:Microsporidiosis / Q000453:epidemiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary
chemical_list: D004271:DNA, Fungal; D016054:DNA, Protozoan