title: Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf).
abstract: The present study was undertaken to produce monoclonal antibodies (MAbs) against immunoglobulin (Ig) purified from black rockfish (Sebastes schlegeli Higendorf) serum using protein A, mannan binding protein, and goat IgG affinity columns. These three different ligands were found to possess high affinity for black rockfish serum Ig. All of the Igs purified eluted at only 0.46 M NaCl concentration in anion exchange column chromatography and consisted of two bands at 70 kDa and 25 kDa in SDS-PAGE; they also had similar antigenicity for MAbs to Ig heavy chain in immunoblot assays. Therefore, black rockfish Ig is believed to exist as a single isotype within serum. The MAbs produced against Ig heavy chain reacted specifically with spots distributed over the pI range from 4.8 to 5.6 with a molecular weight of 70 kDa on two dimensional gel electrophoresis immunoblot profiles.
authors.affiliation: Laboratory of Fish & Shellfish Diseases, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Geewook
authors.initials: G
authors.lastname: Shin
authors.forename: Hyungjun
authors.initials: H
authors.lastname: Lee
authors.forename: K J
authors.initials: KJ
authors.lastname: Palaksha
authors.forename: Youngrim
authors.initials: Y
authors.lastname: Kim
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Lee
authors.forename: Yongseung
authors.initials: Y
authors.lastname: Shin
authors.forename: Eunggoo
authors.initials: E
authors.lastname: Lee
authors.forename: Kyungdae
authors.initials: K
authors.lastname: Park
authors.forename: Taesung
authors.initials: T
authors.lastname: Jung
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002846:Chromatography, Affinity / Q000662:veterinary; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007136:Immunoglobulins / Q000097:blood / Q000276:immunology*; D010473:Perciformes / Q000097:blood / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins