Quantitative analysis of cutaneous malassezia in atopic dermatitis patients using real-time PCR.
We quantified the cutaneous Malassezia in patients with atopic dermatitis using a real-time PCR assay. Seven to 12 times more Malassezia colonized the head and neck compared to the trunk or limbs, and the species M. globosa and M. restricta accounted for approximately 80% of all Malassezia colonization at any body site.
Sugita
Takashi
T
Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. sugita@my-pharm.ac.jp
Tajima
Mami
M
Tsubuku
Hisae
H
Tsuboi
Ryoji
R
Nishikawa
Akemi
A
D000328:Adult; D003876:Dermatitis, Atopic / Q000382:microbiology*; D003881:Dermatomycoses / Q000382:microbiology*; D005260:Female; D006801:Humans; D008290:Malassezia / Q000254:growth & development / Q000302:isolation & purification*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods