Direct evidence for enzyme persulfide and disulfide intermediates during 4-thiouridine biosynthesis.
Two proposed mechanisms for 4-thiouridine generation share key cysteine persulfide and disulfide intermediates, and indirect evidence of their existence has been previously reported; chemical trapping and mass spectrometry have now provided direct and definitive evidence of these key intermediates.
Wright
Chapman M
CM
Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
Christman
Glenn D
GD
Snellinger
Ann M
AM
Johnston
Murray V
MV
Mueller
Eugene G
EG
D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D015394:Molecular Structure; D013891:Thiouridine / Q000737:chemistry / Q000378:metabolism*
D004220:Disulfides; D004798:Enzymes; D013891:Thiouridine; C116840:cysteine persulfide; D003545:Cysteine