title: Analysis of neomycins A, B and C by high-performance liquid chromatography with post-column reaction detection.
abstract: The analysis of the antibiotics neomycins A, B and C was investigated. The separation of the components was studied using reversed-phase and reversed-phase ion-pair chromatography. The optimum separation was obtained utilizing a Lichrosorb RP-2 column with a mobile phase consisting of 75 mg/l sodium dodecyl sulphate, 0.5M Na2SO4 and 0.015 M sodium acetate buffer at pH 7.0. Using this mobile phase, baseline separation was obtained for all three compounds in approximately 20 min. Detection was via post-column derivatization of the analytes with ortho-phthalaldehyde in the presence of mercaptoethanol to form fluorescent iso-indole products. This system is applied to the analysis of a number of formulated products containing neomycin.
authors.affiliation: Department of Analytical Chemistry, Free University, Amsterdam, The Netherlands.
authors.forename: J A
authors.initials: JA
authors.lastname: Apffel
authors.forename: J
authors.initials: J
authors.lastname: Van Der Louw
authors.forename: K R
authors.initials: KR
authors.lastname: Lammers
authors.forename: W T
authors.initials: WT
authors.lastname: Kok
authors.forename: U A
authors.initials: UA
authors.lastname: Brinkman
authors.forename: R W
authors.initials: RW
authors.lastname: Frei
authors.forename: C
authors.initials: C
authors.lastname: Burgess