title: Interaction of glutathione S-transferase with hypericin: A photophysical study.
abstract: The photophysics of hypericin have been studied in its complex with two different isoforms, A1-1 and P1-1, of the protein glutathione S-transferase (GST). One molecule of hypericin binds to each of the two GST subunits. Comparisons are made with our previous results for the hypericin/human serum albumin complex (Photochem. Photobiol. 1999, 69, 633-645). Hypericin binds with high affinity to the GSTs: 0.65 microM for the A1-1 isoform and 0.51 microM for the P1-1 isoform (Biochemistry 2004, 43, 12761-12769). The photophysics and activity of hypericin are strongly modulated by the binding protein. Intramolecular hydrogen-atom transfer is suppressed in both cases. Most importantly, while there is significant singlet oxygen generation from hypericin bound to GST A1-1, binding to GST P1-1 suppresses singlet oxygen generation to almost negligible levels. The data are rationalized in terms of a simple model in which the hypericin photophysics depends entirely upon the decay of the triplet state by two competing processes, quenching by oxygen to yield singlet oxygen and ionization, the latter of these two are proposed to be modulated by A1-1 and P1-1.
authors.affiliation: Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: M
authors.initials: M
authors.lastname: Halder
authors.forename: P K
authors.initials: PK
authors.lastname: Chowdhury
authors.forename: R
authors.initials: R
authors.lastname: Das
authors.forename: P
authors.initials: P
authors.lastname: Mukherjee
authors.forename: W M
authors.initials: WM
authors.lastname: Atkins
authors.forename: J W
authors.initials: JW
authors.lastname: Petrich
mesh_terms: D000873:Anthracenes; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004121:Dimethyl Sulfoxide; D005982:Glutathione Transferase / Q000737:chemistry*; D007700:Kinetics; D008027:Light; D010100:Oxygen / Q000737:chemistry; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D010777:Photochemistry; D010782:Photolysis; D011522:Protons; D012709:Serum Albumin / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000873:Anthracenes; D011522:Protons; D012709:Serum Albumin; D010569:Perylene; C004965:hypericin; D005982:Glutathione Transferase; D010100:Oxygen; D004121:Dimethyl Sulfoxide