[Extracellular proteinases from the phytopathogenic fungus Fusarium culmorum].
The growth of Fusarium culmorum fungus on a medium containing thermostable proteins from potato tubers was accompanied by the production of proteinases, exhibiting activity over a broad pH range (from 6.0-10.0). When studied by SDS-PAGE in the presence of beta-mercaptoethanol, extracellular proteinases were represented by at least five species with a molecular weight of 30-60 kDa. Inhibitor analysis and studies of enzyme activities with synthetic substrates demonstrated that the culture liquid of Fusarium culmorum contained serine proteinases of various classes. The amount of subtilisin-like proteinases was the highest. A near-complete inhibition of the enzymes was caused by proteinaceous proteinase inhibitors from potato tubers. These data suggest that proteinases of the phytopathogen Fusarium culmorum serve as a metabolic target for natural inhibitors of potato proteinases.
Ievleva
E V
EV
Revina
T A
TA
Kudriavtseva
N N
NN
Sof'in
A V
AV
Valueva
T A
TA
D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology* / Q000254:growth & development; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D011480:Protease Inhibitors / Q000494:pharmacology; D011198:Solanum tuberosum / Q000737:chemistry / Q000382:microbiology
D005656:Fungal Proteins; D011480:Protease Inhibitors; D010447:Peptide Hydrolases