Membrane's Eleven: heavy-atom derivatives of membrane-protein crystals.
A database has been assembled of heavy-atom derivatives used in the structure determination of membrane proteins. The database can serve as a guide to the design of experiments in the search for heavy-atom derivatives of new membrane-protein crystals. The database pinpoints organomercurials, platinum(II) and trimethyllead compounds as being particularly useful. On the other hand, lanthanide and uranyl compounds are poorly represented, which may be a consequence of these compounds having aggressive effects in crystal-soaking procedures. Furthermore, the database highlights the variety of methods applied in the preparation of heavy-atom-derivatized crystals and in phasing. Cocrystallization can be further exploited. Phases have predominantly been obtained by SIRAS/MIRAS methods rather than SAD/MAD in recent structure determinations.
Morth
J Preben
JP
Center for Structural Biology, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK-8000 Aarhus C, Denmark.
Sørensen
Thomas Lykke Møller
TL
Nissen
Poul
P
D018360:Crystallography, X-Ray; D030562:Databases, Protein; D008565:Membrane Proteins / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry*; D009941:Organomercury Compounds / Q000737:chemistry*; D017433:Protein Structure, Secondary
D008565:Membrane Proteins; D019216:Metals, Heavy; D009941:Organomercury Compounds