Determination of flunixin in equine plasma by reversed-phase liquid chromatography.
Flunixin is determined in equine plasma by liquid chromatography on LiChrosorb RP-18 with 70% methanol in phosphate buffer pH 3.1 as the eluent, with detection at 284 nm. Plasma is deproteinized with methanol and the supernatant is then injected directly into the system. With a short pre-column (5 x 3 mm i.d.), which is replaced after 25-40 injections of sample, 420 plasma samples could be analysed on one analytical column. The detection limit in plasma is 0.30 micromol/l (89 ng/ml) and the method can be used in pharmacokinetic studies.
Johansson
I M
IM
Swedish University of Agricultural Sciences, Department of Pharmacology, Faculty of Veterinary Medicine, Biomedical Centre, Box 573, S-751 23 Uppsala, Sweden.
Schubert
B
B