Expression of intracellular adhesion molecule-1 linking superoxide to mobilization of granulocytes and macrophages after periarterial blood in rat femoral artery: effect of rebamipide.
OBJECTIVE
To clarify the mechanism(s) involved in the perivascular mobilization of granulocytes and macrophages by periarterial autologous blood (PAAB) in the vicinity of the femoral artery (FA) in rats, superoxide production as well as expression of intercellular adhesion molecule-1 (ICAM-1) were determined by conducting both in vitro and in vivo experiments.


METHODS
In an in vitro study, a significant amount of superoxide inhibited by diphenyleneiodonium (20 microM and 100 microM) was identified at 3 hours after application of 10% whole blood to the aortic segments, and these results were correlated with in vitro ICAM-1 expression. High expression of ICAM-1 was subsequently demonstrated in these segments at 24 hours in in vitro and in vivo studies. In the in vivo study, an increased mobilization of granulocytes paralleled with a high expression of ICAM-1 in the vessels at 24 hours after administration of PAAB to the FA and then declined. Subsequently, macrophage infiltration progressively increased at all layers throughout a period of 7 to 12 days. Pretreatment with rebamipide (100 and 300 mg kg(-1) day(-1), orally) significantly inhibited the expression of ICAM-1 with inhibition of mobilization of granulocyte/macrophage.


CONCLUSIONS
These findings suggest that application of PAAB to the rat FA causes superoxide-linked expression of ICAM-1 and mobilization of granulocyte and macrophages. Thus, the potential value in suppressing these variables stimulated by PAAB is indicated in therapeutic strategies for prevention and possible regression of vasospasm after subarachnoid hemorrhage.
Choi
J M
JM
Department of Pharmacology, College of Medicine, Pusan National University, Pusan, Korea.
Kim
C D
CD
Hong
K W
KW
D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005263:Femoral Artery / Q000187:drug effects / Q000502:physiology*; D015870:Gene Expression / Q000187:drug effects*; D006098:Granulocytes / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D015363:Quinolones / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000378:metabolism*
D000975:Antioxidants; D015363:Quinolones; D013481:Superoxides; D018799:Intercellular Adhesion Molecule-1; C052785:rebamipide; D000409:Alanine