[Effect of rmu-IFN-gamma on the splenic T cell subsets and NK cells of pregnant mice infected with Toxoplasma gondii].
OBJECTIVE
To explore the effect of rmu-IFN-gamma on the change of T cell subsets and natural killer cells of pregnant mice infected with T. gondii.


METHODS
Early pregnant mice infected with T. gondii were administered with different doses (1 U/g or 10 U/g) of rmu-IFN-gamma for three days before euthanasia. The numbers of splenic CD4+ and CD8+ T cells and natural killer cells were detected by flow cytometry.


RESULTS
Compared with the infected mice that were not treated, the level of splenic CD4+ T cells in mice administered with two doses of rmu-IFN-gamma increased on the day 10, 12, 14 of gestation, while the level of CD8+ T cells decreased on the day 10, 14 of gestation. The ratio of CD4+/CD8+ T cells increased significantly on the day 10, 12, 14 of gestation. Survival days of the two administered groups were longer than those of the infected group.


CONCLUSIONS
A proper dose of rmu-IFN-gamma can reverse the decline of the ratio of T cell subsets, improve the proliferation of NK cells, and so increase the level of peripheral cellular immunity of pregnant mice.
Luo
Fang-jun
FJ
Department of Clinical Laboratory, Zhuji People's Hospital, Zhuji 311800, China.
D000818:Animals; D016516:CD4-CD8 Ratio; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology* / Q000627:therapeutic use; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D011994:Recombinant Proteins; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects*; D014122:Toxoplasma / Q000187:drug effects* / Q000254:growth & development; D014124:Toxoplasmosis, Animal / Q000188:drug therapy* / Q000276:immunology / Q000469:parasitology
D011994:Recombinant Proteins; D007371:Interferon-gamma