Solution conformation of an immunodominant epitope in the hepatitis B virus preS2 surface antigen.
We have determined the solution conformation of the major B cell epitope (residues 123-145, adrl23 hereafter) in the preS2 region of hepatitis B virus known to be associated with infection neutralization. The adrl23 shows an "L" shaped helix-turn-helix topology with two beta-turns formed by residues Ala(130)-Asp(133) and Asp(133)-Val(136) intervening the N- and C-terminal helices. The N-terminal alpha-helix consists of residues Ser(124)-Gln(129) whereas the C-terminal 3(10) helix is formed by residues Val(136)-Tyr(140). The beta-turns overlap partially with the putative "conformational" epitope. The overall topology of adrl23 is primarily maintained by hydrophobic interactions involving Phe(127), Leu(131), Leu(132), Val(136), and Tyr(140) that are clustered on one side of the molecule. An additional hydrophobic stabilization comes from Phe(141) that is buried inside the concave side of the molecule. A network of hydrogen bonds formed among Thr(125), His(128), and Arg(137) further contribute to the "boomerang-shaped" architecture of adrl23. The N-terminus of adrl23 is immobile due to a hydrogen bond between the N-terminal amide proton of Asn(123) and the hydroxyl oxygen of Thr(126). The side chains of Asp(133), Arg(135), Val(136), Leu(139), and Tyr(140) that were shown to be important for binding to a monoclonal antibody H8 mAb are surface exposed.
Chi
Seung-Wook
SW
Molecular Cancer Research Center, Division of Molecular Therapeutics, Korea Research Institute of Bioscience and Biotechnology, Yusong P.O. Box 115, Daejon, Korea.
Kim
Do-Hyoung
DH
Kim
Jae-Sung
JS
Lee
Myung Kyu
MK
Han
Kyou-Hoon
KH
D019077:Helix-Turn-Helix Motifs; D006514:Hepatitis B Surface Antigens / Q000737:chemistry* / Q000276:immunology; D006515:Hepatitis B virus / Q000235:genetics*; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D016056:Immunodominant Epitopes / Q000737:chemistry*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D011498:Protein Precursors / Q000737:chemistry* / Q000276:immunology; D017434:Protein Structure, Tertiary
D006514:Hepatitis B Surface Antigens; D016056:Immunodominant Epitopes; D011498:Protein Precursors; C050807:presurface protein 2, hepatitis B surface antigen