Migratory dendritic cells transfer antigen to a lymph node-resident dendritic cell population for efficient CTL priming.
Skin dendritic cells (DCs) are thought to act as key initiators of local T cell immunity. Here we show that after skin infection with herpes simplex virus (HSV), cytotoxic T lymphocyte (CTL) activation required MHC class I-restricted presentation by nonmigratory CD8(+) DCs rather than skin-derived DCs. Despite a lack of direct presentation by migratory DCs, blocking their egress from infected skin substantially inhibited class I-restricted presentation and HSV-specific CTL responses. These results support the argument for initial transport of antigen by migrating DCs, followed by its transfer to the lymphoid-resident DCs for presentation and CTL priming. Given that relatively robust CTL responses were seen with small numbers of skin-emigrant DCs, we propose that this inter-DC antigen transfer functions to amplify presentation across a larger network of lymphoid-resident DCs for efficient T cell activation.
Allan
Rhys S
RS
The Department of Microbiology and Immunology, The University of Melbourne, Parkville 3010, Victoria, Australia.
Waithman
Jason
J
Bedoui
Sammy
S
Jones
Claerwen M
CM
Villadangos
Jose A
JA
Zhan
Yifan
Y
Lew
Andrew M
AM
Shortman
Ken
K
Heath
William R
WR
Carbone
Francis R
FR
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D002465:Cell Movement; D002478:Cells, Cultured; D045142:Cross-Priming; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D006561:Herpes Simplex / Q000276:immunology / Q000821:virology; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D018139:Simplexvirus / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000941:Antigens