Factors affecting iron sulfide-enhanced bacteriophage plaque assays in Salmonella.
Reaction of ferric ions with hydrogen sulfide (H(2)S) enhances contrast of phage plaques in H(2)S+ Salmonella, but contrast diminishes in weak H(2)S+ strains. H(2)S was affected by concentrations of peptones, glucose, ferric ammonium citrate (FAC) and sodium thiosulfate (ST), and by FAC:ST ratio, temperature, pH, air, and host strain. Increasing peptone levels was most important for improving contrast in weak H(2)S+ strains.
McLaughlin
M R
MR
United States Department of Agriculture, Agricultural Research Service, Waste Management and Forage Research Unit, Mississippi State, Mississippi 39762-5367, USA. mmclaughlin@ars.usda.gov <mmclaughlin@ars.usda.gov>
D003470:Culture Media / Q000737:chemistry; D006862:Hydrogen Sulfide / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D012475:Salmonella / Q000378:metabolism / Q000821:virology*; D012483:Salmonella Phages / Q000254:growth & development*; D013696:Temperature; D010948:Viral Plaque Assay / Q000379:methods*
D003470:Culture Media; D006862:Hydrogen Sulfide