Characterization and comparison of the chromatographic performance of different types of reversed-phase stationary phases.
The chromatographic performance of several base-deactivated stationary phases was evaluated with a specific chromatographic test. Seven basic test compounds, possessing different physico-chemical properties were injected on different supports with two mobile phases: one at pH 7.0 (acetonitrile-phosphate buffer, 40:60, v/v), and the other at pH 3.0 (acetonitrile-phosphate buffer, 15:85, v/v). Chromatographic parameters obtained under these conditions were treated by principal component analysis (PCA) to separate base deactivated supports according to their silanol activity (pH 7.0 mobile phase) and hydrophobic properties (pH 3.0 mobile phase). The information given by the specific test column evaluation was improved with complementary chemometric tools such as hierarchical cluster analysis. The same base deactivated supports were also tested following a general test procedure issued from the literature and obtained fundamental properties (in particular silanol activity and hydrophobicity) were compared with column evaluation obtained with the specific test: results were in good agreement, although the use of the specific test offered a better differentiation between numerous base-deactivated supports.
Stella
Cinzia
C
Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences, University of Geneva, Switzerland.
Rudaz
Serge
S
Gauvrit
Jean-Yves
JY
Lantéri
Pierre
P
Huteau
Alban
A
Tchapla
Alain
A
Veuthey
Jean-Luc
JL
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography / Q000295:instrumentation* / Q000706:statistics & numerical data; D016000:Cluster Analysis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010316:Particle Size; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry; D012984:Software
D007202:Indicators and Reagents; D012821:Silanes; C082343:silanol