Simple identification of transgenic Arabidopsis plants carrying a single copy of the integrated gene.
Transgenic Arabidopsis thaliana plants carrying a single copy of integrated DNA can be identified by single-step genomic polymerase chain reaction. The reaction employs two sets of primer pairs with the same melting temperature that amplify the amplicons derived from the integrated T-DNA together with those from an endogenous single-copy gene as a reference. When the band intensity ratio is one, this means that the transgenic plants are carrying a single copy of the integrated gene per haploid.
Kihara
Tomonori
T
Forestry Research Institute, Oji Paper, Kameyama, Mie 519-0212, Japan.
Zhao
Cheng-Ri
CR
Kobayashi
Yuriko
Y
Takita
Eiji
E
Kawazu
Tetsu
T
Koyama
Hiroyuki
H
D017360:Arabidopsis / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D018628:Gene Dosage; D018745:Genome, Plant; D006579:Heterozygote; D006720:Homozygote; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction
D018744:DNA, Plant