Affinity-based assay of Rho guanosine triphosphatase activation.
The recognition that Rho guanosine triphosphatases (GTPases) (Rho, Rac, and Cdc42) play important regulatory roles in many areas of cell biology has made the ability to measure their activity in cells an important biological tool. Because Rho GTPases become activated by conversion from guanosine diphosphate-bound states to guanosine triphosphate (GTP)-bound forms, affinity-based methods to detect the formation of GTP-Rho GTPases have been developed and are widely used for the purpose of assessing Rho GTPase activities in biological studies.
Stofega
Mary
M
Department of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA, USA.
DerMardirossian
Celine
C
Bokoch
Gary M
GM
D051017:Apoptosis Regulatory Proteins; D001681:Biological Assay / Q000379:methods*; D004789:Enzyme Activation; D019204:GTP-Binding Proteins; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D054462:p21-Activated Kinases; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; C100494:RTKN protein, human; D011993:Recombinant Fusion Proteins; C515903:PAK1 protein, human; D017346:Protein Serine-Threonine Kinases; D054462:p21-Activated Kinases; D019204:GTP-Binding Proteins; D020741:rho GTP-Binding Proteins