Sedimentation field flow fractionation monitoring of bimodal wheat starch amylolysis.
Enzymatic starch granule hydrolysis is one of the most important reactions in many industrial processes. In this study, we investigated the capacity of sedimentation field flow fractionation (SdFFF) to monitor the amylolysis of a bimodal starch population: native wheat starch. Results demonstrated a correlation between fractogram changes and enzymatic hydrolysis. Furthermore, SdFFF was used to sort sub-populations which enhanced the study of granule size distribution changes occurring during amylolysis. These results show the interest in coupling SdFFF with particle size measurement methods to study complex starch size/density modifications associated to hydrolysis. These results suggested different applications such as the association of SdFFF with structural investigations to better understand the specific mechanisms of amylolysis or starch granule structure.
Salesse
C
C
Laboratoire de Chimie Analytique, EA 3842 Homéostasie Cellulaire & Pathologies, Université de Limoges, Faculté de Pharmacie, 2 Rue du Dr Marcland, Limoges Cedex, France.
Battu
S
S
Begaud-Grimaud
G
G
Cledat
D
D
Cook-Moreau
J
J
Cardot
P J P
PJ
D000681:Amylases / Q000378:metabolism; D031521:Fractionation, Field Flow / Q000295:instrumentation / Q000379:methods*; D006868:Hydrolysis; D010316:Particle Size; D015203:Reproducibility of Results; D013213:Starch / Q000032:analysis* / Q000378:metabolism; D014908:Triticum / Q000737:chemistry*
D013213:Starch; D000681:Amylases