title: Pentagastrin-induced nitric oxide-dependent protein secretion from the parotid gland of the anaesthetized rat.
abstract: Infusion of pentagastrin (20 microg kg(-1) h(-1), i.v.) for 10 min evokes protein output but no overt fluid secretion from the parotid gland of the rat, as revealed by increased protein concentration in a subsequent wash-out flow of saliva in response to a bolus injection of methacholine (5 microg kg(-1), i.v.) 10 min later. Using this experimental set-up, the contribution of nitric oxide (NO) generation to the protein and amylase response evoked by pentagastrin was investigated. Neither the neuronal type NO synthase inhibitor N(omega)-propyl-L-arginine (N-PLA; 30 mg kg(-1), i.v.) nor the non-selective NO synthase inhibitor L-NAME (30 mg kg(-1), i.v.) as such affected the methacholine-evoked volume response or the outputs of protein and amylase. However, when preceeded by the pentagastrin infusion, the expected increases in concentrations of protein (145%) and amylase activity (127%) of the methacholine-evoked response (compared to a pre-infusion methacholine response) were reduced to 68 and 74%, respectively, in the presence of N-PLA, and to 70 and 63%, respectively, in the presence of L-NAME. Thus, NO generation resulting from the activity of the neuronal type NO synthase, most probably of parenchymal origin, plays an important role in the pentagastrin-induced protein and amylase secretion of the rat parotid gland.
authors.affiliation: Department of Pharmacology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at Göteborg University, Medicinaregatan 15 D, 405 30 Göteborg, Sweden.
authors.forename: Hülya Cevik
authors.initials: HC
authors.lastname: Aras
authors.forename: J
authors.initials: J
authors.lastname: Ekström
mesh_terms: D000681:Amylases / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D016210:Methacholine Chloride / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D010418:Pentagastrin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D027544:Salvia / Q000378:metabolism; D014474:Unconsciousness / Q000503:physiopathology*
chemical_list: C511598:Bpifa2 protein, rat; D004791:Enzyme Inhibitors; C109599:N(omega)-propylarginine; D012471:Salivary Proteins and Peptides; D016210:Methacholine Chloride; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D000681:Amylases; D010418:Pentagastrin; D019331:NG-Nitroarginine Methyl Ester