Spores from mesophilic Bacillus cereus strains germinate better and grow faster in simulated gastro-intestinal conditions than spores from psychrotrophic strains.
The species Bacillus cereus, known for its ability to cause food borne disease, consists of a large variety of strains. An important property for discrimination of strains is their growth temperature range. Psychrotrophic strains can grow well at refrigerator temperatures but grow at 37 degrees C with difficulty. Mesophilic strains on the other hand are unable to grow below 10 degrees C, but grow well at 37 degrees C. Spores of six psychrotrophic and six mesophilic strains were investigated for their ability to survive and grow in simulated gastro-intestinal fluids, mimicking the conditions in the gastro-intestinal tract. The germination potential of psychrotrophic and mesophilic spores in simulated intestinal fluid does not differ much. Under conditions simulating the gastro-intestinal passage, 5 out of 6 mesophilic strains showed growth, and only 2 out of 6 psychrotrophic strains. Temperature (37 degrees C) and simulated gastro-intestinal conditions together influenced germination and growth.
Wijnands
L M
LM
Microbiological Laboratory for Health Protection, National Institute for Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. lucas.wijnands@rivm.nl
Dufrenne
J B
JB
Zwietering
M H
MH
van Leusden
F M
FM
D001409:Bacillus cereus / Q000254:growth & development / Q000502:physiology*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000517:prevention & control; D006863:Hydrogen-Ion Concentration; D013171:Spores, Bacterial / Q000254:growth & development; D013696:Temperature; D013997:Time Factors
D003470:Culture Media