[Effects of voltage during anodization on titanium surface on cell attachment and spreading of osteoblasts].
OBJECTIVE
The effects of the voltage during anodic oxidation on cell attachment and spreading of human osteoblasts in early stage were investigated.


METHODS
The samples were anodized in the electrolyte of 0.03 M calcium glycerophosphate (Ca-GP) mixed with 0.15 M calcium acetate (CA) under current density of 70 A/m2 and different voltages, 140V, 200V or 260V. The surface roughness of the specimens and the attachment and spreading of human osteoblasts in early stage were investigated. SPSS13.0 for windows was used for one-way ANOVA.


RESULTS
The surface average roughness of cp Ti was significantly improved from 0.17 micro m to 0.23 micro m, 0.26 micro m, 0.33 micro m, respectively, by anodic oxidation with increased voltage (P<0.05). After 2 hours of cell culture, cell skeleton was reorganized and cell morphology became more irregular with the increase of anodizing voltages. Cell attachment on the specimens anodized under 260V was significantly higher than on the surface of cp Ti (P<0.05).


CONCLUSIONS
The surface properties of cp Ti can be affected by anodic oxidation. The early cell attachment and spreading were enhanced with anodizing voltage.
Chen
Jun
J
Hangzhou Dental Hospital, Hangzhou 310015, Zhejiang Province, China. chenj99@gmail.com
Zhu
Xiao-long
XL
Scheideler
L
L
Geis-Gerstorfer
J
J
D000085:Acetates; D017610:Calcium Compounds; D002448:Cell Adhesion / Q000502:physiology*; D004563:Electrochemistry; D004595:Electroplating / Q000379:methods; D006801:Humans; D010006:Osteoblasts / Q000502:physiology*; D010084:Oxidation-Reduction; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D000085:Acetates; D017610:Calcium Compounds; D014025:Titanium; C120662:calcium acetate