title: Adipose gene expression response of lean and obese mice to short-term dietary restriction.
abstract: Overweight and obesity lead to higher morbidity risks, which are alleviated even by mild weight loss. To gain insight in the molecular effects of weight loss in adipose tissue, we analyzed the effects of short-term dietary restriction (DR) on mice fed a low-fat diet (lean mice) or a high-fat diet (obese mice). Female C57Bl6/J mice on both diets were on DR until an average body weight loss of 20%, which was achieved in 8 to 12 days depending on body weight at the start of DR. Plasma free fatty acids and blood glucose levels decreased significantly on DR. In the (restricted) low-fat diet groups, gene expression analysis using adipose-enriched cDNA microarrays revealed only two transcripts to be significant differentially expressed by DR: up-regulation of malic enzyme (Mod1) and down-regulation of major urinary protein 1 (Mup1). Real-time polymerase chain reaction analysis confirmed these findings and showed, for the high-fat diet groups, an identical expression pattern for Mup1, whereas Mod1 showed an opposed gene expression pattern for the high-fat diet groups. In conclusion, initial weight loss induces transcriptional changes only in a very small number of adipose genes, which also depends on the (restricted) diet used.
authors.affiliation: Laboratory of Toxicology, Pathology, and Genetics, National Institute of Public Health and the Environment, Bilthoven, The Netherlands. Evert.van.Schothorst@rivm.nl
authors.forename: Evert M
authors.initials: EM
authors.lastname: van Schothorst
authors.forename: Jaap
authors.initials: J
authors.lastname: Keijer
authors.forename: Jeroen L A
authors.initials: JL
authors.lastname: Pennings
authors.forename: Antoon
authors.initials: A
authors.lastname: Opperhuizen
authors.forename: Charissa E
authors.initials: CE
authors.lastname: van den Brom
authors.forename: Thomas
authors.initials: T
authors.lastname: Kohl
authors.forename: Nicole L W
authors.initials: NL
authors.lastname: Franssen-van Hal
authors.forename: Barbara
authors.initials: B
authors.lastname: Hoebee
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D031204:Caloric Restriction / Q000379:methods; D004036:Diet, Atherogenic; D018752:Diet, Fat-Restricted; D005260:Female; D005786:Gene Expression Regulation; D008291:Malate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000378:metabolism; D013851:Thinness / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011506:Proteins; C035737:major urinary proteins; D008291:Malate Dehydrogenase