The expression of the carboxyl ester lipase gene in pancreas and pancreatic adenocarcinomas.
Since pancreatic cancer is an aggressive and often incurable malignancy, we investigated if the carboxyl ester lipase gene (CEL) is specifically expressed in pancreatic tissues and its promoter can be used for a specific suicide gene approach. Twenty-five tumor samples, 24 samples of normal pancreatic tissue and control tissues from other organs were examined by radioactive in situ hybridization (ISH) to localize CEL mRNA. Two carcinoma samples and 6 permanent cell lines were examined by reverse transcriptase-polymerase chain reaction (RT-PCR). By ISH, we verified a strong CEL gene expression in acinar cells of the normal pancreas. A minor expression was noted in a single sample of acinar cell carcinoma and adenocarcinomas did not show any expression. By RT-PCR, no specific expression in both tested adenocarcinomas was observed. In summary, these results show that, contrary to notable expression of carboxyl ester lipase in acinar cells of normal pancreatic tissue, this lipase is not significantly active in pancreatic adenocarcinomas and thus not an apt genetic marker for diagnostic or therapeutic approaches.
Reuss
Reinhard
R
Division of Molecular Genetics, Institute of Anthropology and Human Genetics, University Hospital Tübingen, Eberhard-Karls University Tübingen, D-72076 Tübingen, Germany. reinhard.reuss@neuro.med.uni-giessen.de
Aberle
Susanne
S
Klingel
Karin
K
Sauter
Martina
M
Greschniok
Annette
A
Franke
Folker Ernst
FE
Padberg
Winfried
W
Blin
Nikolaus
N
D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics; D002288:Adenocarcinoma, Mucinous / Q000201:enzymology / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D018267:Carcinoma, Acinar Cell / Q000201:enzymology / Q000235:genetics; D021441:Carcinoma, Pancreatic Ductal / Q000201:enzymology / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D017403:In Situ Hybridization; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics / Q000556:secondary; D010179:Pancreas / Q000201:enzymology*; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000235:genetics; D010195:Pancreatitis / Q000201:enzymology / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D015347:RNA Probes; D012333:RNA, Messenger; C054621:CEL protein, human; D008049:Lipase