Acridine orange staining and viability of the coccoid form of Campylobacter upsaliensis.
Conversion of Campylobacter upsaliensis to the coccoid form during aerobic incubation at 37 degrees C was not prevented by treatment with chloramphenicol and was accompanied by severe decreases in isocitrate dehydrogenase activity and oxygen uptake. Although the coccoid forms fluoresced orange-red by acridine orange staining, agarose gel electrophoresis indicated an extensive degradation of the ribosomal RNA. This suggests that acridine orange staining may not be a good indicator of viability and that the coccoid form of C. upsaliensis at 37 degrees C is degenerative rather than part of the life cycle.
Santiwatanakul
Somchai
S
Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Thaveewattana, Bangkok 10300,Thailand. somchaii@swu.ac.th
Krieg
Noel R
NR
D000165:Acridine Orange; D044885:Campylobacter upsaliensis / Q000502:physiology* / Q000648:ultrastructure; D005456:Fluorescent Dyes; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D050296:Microbial Viability; D010101:Oxygen Consumption / Q000502:physiology*; D012335:RNA, Ribosomal / Q000502:physiology*
D005456:Fluorescent Dyes; D012335:RNA, Ribosomal; D007521:Isocitrate Dehydrogenase; D000165:Acridine Orange