title: Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae.
abstract: Yarrowia lipolytica was recently introduced as a new model organism to study peroxisome degradation in yeasts. Transfer of Y. lipolytica cells from oleate/ethylamine to glucose/ammonium chloride medium leads to selective macroautophagy of peroxisomes. To decipher the molecular mechanisms of macropexophagy we isolated mutants of Y. lipolytica defective in the inactivation of peroxisomal enzymes under pexophagy conditions. Through this analysis we identified the gene YlTRS85, the ortholog of Saccharomyces cerevisiae TRS85 that encodes the 85 kDa subunit of transport protein particle (TRAPP). A parallel genetic screen in S. cerevisiae also identified the trs85 mutant. Here, we report that Trs85 is required for nonspecific autophagy, pexophagy and the cytoplasm to vacuole targeting pathway in both yeasts.
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine.
authors.forename: Taras Y
authors.initials: TY
authors.lastname: Nazarko
authors.forename: Ju
authors.initials: J
authors.lastname: Huang
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Nicaud
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Klionsky
authors.forename: Andrei A
authors.initials: AA
authors.lastname: Sibirny
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism; D001343:Autophagy; D000071190:Autophagy-Related Protein 8 Family; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009154:Mutation; D020675:Peroxisomes / Q000502:physiology; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D015398:Signal Transduction; D014617:Vacuoles / Q000502:physiology*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*; D025062:Yarrowia / Q000378:metabolism*
chemical_list: C113757:ATG8 protein, S cerevisiae; D000071190:Autophagy-Related Protein 8 Family; D005656:Fungal Proteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; C406327:transport protein particle, TRAPP; D000626:Aminopeptidases; C019689:APE1 protein, S cerevisiae