title: The Hansenula polymorpha ATG25 gene encodes a novel coiled-coil protein that is required for macropexophagy.
abstract: We have isolated the Hansenula polymorpha ATG25 gene, which is required for glucose-induced selective peroxisome degradation by macropexophagy. ATG25 represents a novel gene that encodes a 45 kDa coiled-coil protein. We show that this protein colocalizes with Atg11 on a small structure, which most likely represents the pre-autophagosomal structure (PAS). In cells of a constructed ATG25 deletion strain (atg25) peroxisomes are constitutively degraded by nonselective microautophagy, a process that in WT H. polymorpha is only observed at nitrogen limitation conditions. This suggests that nonselective microautophagy is deregulated in H. polymorpha atg25 cells.
authors.affiliation: Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.
authors.forename: Iryna
authors.initials: I
authors.lastname: Monastyrska
authors.forename: Jan A K W
authors.initials: JA
authors.lastname: Kiel
authors.forename: Arjen M
authors.initials: AM
authors.lastname: Krikken
authors.forename: Janet A
authors.initials: JA
authors.lastname: Komduur
authors.forename: Marten
authors.initials: M
authors.lastname: Veenhuis
authors.forename: Ida J
authors.initials: IJ
authors.lastname: van der Klei
mesh_terms: D001343:Autophagy; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D020675:Peroxisomes / Q000378:metabolism / Q000648:ultrastructure; D010843:Pichia / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D005656:Fungal Proteins