title: Cell-directed assembly of lipid-silica nanostructures providing extended cell viability.
abstract: Amphiphilic phospholipids were used to direct the formation of biocompatible, uniform silica nanostructures in the presence of Saccharomyces cerevisiae and bacterial cell lines. The cell surfaces organize multilayered phospholipid vesicles that interface coherently with the silica host and help relieve drying stresses that develop with conventional templates. These host structures maintain cell accessibility, addressability, and viability in the absence of buffer or an external fluidic architecture. The cell surfaces are accessible and can be used to localize added proteins, plasmids, and nanocrystals. Prolonged cell viability combined with reporter protein expression enabled stand-alone cell-based sensing.
authors.affiliation: Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, NM 87185, USA.
authors.forename: Helen K
authors.initials: HK
authors.lastname: Baca
authors.forename: Carlee
authors.initials: C
authors.lastname: Ashley
authors.forename: Eric
authors.initials: E
authors.lastname: Carnes
authors.forename: Deanna
authors.initials: D
authors.lastname: Lopez
authors.forename: Jeb
authors.initials: J
authors.lastname: Flemming
authors.forename: Darren
authors.initials: D
authors.lastname: Dunphy
authors.forename: Seema
authors.initials: S
authors.lastname: Singh
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Nanguo
authors.initials: N
authors.lastname: Liu
authors.forename: Hongyou
authors.initials: H
authors.lastname: Fan
authors.forename: Gabriel P
authors.initials: GP
authors.lastname: López
authors.forename: Susan M
authors.initials: SM
authors.lastname: Brozik
authors.forename: Margaret
authors.initials: M
authors.lastname: Werner-Washburne
authors.forename: C Jeffrey
authors.initials: CJ
authors.lastname: Brinker
mesh_terms: D001412:Bacillus subtilis / Q000502:physiology*; D002021:Buffers; D002462:Cell Membrane; D018914:Cells, Immobilized; D004926:Escherichia coli / Q000502:physiology*; D036681:Fluorescence Recovery After Photobleaching; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers; D008823:Micelles; D008854:Microscopy, Electron; D049329:Nanostructures; D010743:Phospholipids; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D012542:Scattering, Radiation; D012822:Silicon Dioxide; D014965:X-Rays
chemical_list: D002021:Buffers; D008051:Lipid Bilayers; D008823:Micelles; D010743:Phospholipids; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D012822:Silicon Dioxide