[An epidemiological investigation of bats carrying SARS-CoV in Guangzhou and its vicinity].
OBJECTIVE
To detect serve acute respiratory syndrome-associated coronavirus (SARS-CoV) and SARS-like-CoV in fruit bats captured in Guangzhou and its vicinity.


METHODS
Totally 927 bats of 9 species (Cynopterus sphinx, Rousettus leschenaulti, Miniopterus schreibersi, Hipposideros pratti, Rhinolophusasinicus, Scotophilusakuhlii, Hipposideros Pomona, Rhinolophus affinis, and Rhinolophus pusillus) captured in Guangzhou and its vicinity from September 2004 to November 2005 were available for this investigation, from which 3,043 samples (813 throat swasb, 524 sera, 853 lung tissues and 853 colorectal tissue specimens) were obtained. SARS-Cov and SARS-like-CoV were detected in these specimens using diagnostic kit for novel coronavirus N protein (ELISA), SARS-CoV Virus RNA detection kit, fluorescence PCR, Genchip, RT-PCR and cell isolation culture methods.


CONCLUSIONS
No SARS-CoV and SARS-like-CoV were detected in the 3043 samples, indicating the current absence of SARS-CoV and SARS-like-CoV in the bats captured in Guangzhou and its vicinity.
Li
Zhi-feng
ZF
Department of Epidemiology, Southern Medical University, Guangzhou 510515, China.
Hu
Yong
Y
Zhan
Hui-chun
HC
Yun
Xue-xia
XX
Du
Yu-ping
YP
Ke
Xue-mei
XM
Yu
De-xian
DX
Li
Jian-dong
JD
Dai
Ying-chun
YC
Chen
Qing
Q
Yu
Shou-yi
SY
D000818:Animals; D002681:China / Q000453:epidemiology; D002685:Chiroptera / Q000821:virology*; D000086462:Coronavirus Nucleocapsid Proteins; D004199:Disease Vectors; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019590:Nucleocapsid Proteins / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D045169:Severe Acute Respiratory Syndrome / Q000453:epidemiology / Q000635:transmission / Q000821:virology
D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D012367:RNA, Viral