[Overexpression of PEMT2 inhibits the phosphorylation and translocation of PLC gamma 1].
OBJECTIVE
To explore the mechanism of CBRH-7919 cell proliferation inhibition by transfecting phosphatidylethanolamine N-methyltransferase 2 gene (PEMT2).


METHODS
The effects of PEMT2 transfection on phosphorylation and translocation from cytosol to plasma membrane of PLC gamma 1 in cells were studied using SDS-PAGE and Western blot techniques. The phosphorylation and activity of c-Met were determined.


RESULTS
After transfection of pemt2, the PLC gamma 1 and phosphorylated PLC gamma 1 conjugated with plasma membrane were decreased by 45% and 27% of that of control cells respectively, and the phosphorylated c-Met was decreased to 32% of that of control cells.


CONCLUSIONS
Transfection of phosphatidylethanolamine N-methyltransferase 2 gene can inhibit the phosphorylation and translocation from cytosol to plasma membrane of PLC gamma 1 in cells. At the same time, the autophosphorylation of c-Met was decreased, which suggests that transfection of phosphatidylethanolamine N-methyltransferase 2 gene can downregulate the c-Met/PLC gamma 1 signaling pathway in CBRH-7919 cells.
Li
Ya-li
YL
Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116027, China.
Shao
Yan-hua
YH
Liu
Zhi-li
ZL
Xia
Quan
Q
Ma
Ke-li
KL
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D008114:Liver Neoplasms, Experimental; D050918:Phosphatidylethanolamine N-Methyltransferase / Q000235:genetics* / Q000378:metabolism; D051966:Phospholipase C gamma / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D051381:Rats; D014162:Transfection
D050918:Phosphatidylethanolamine N-Methyltransferase; D019859:Proto-Oncogene Proteins c-met; D051966:Phospholipase C gamma