Cross-crystallization method used for the crystallization and preliminary diffraction analysis of a novel di-haem cytochrome c4.
The newly discovered di-haem cytochrome c4 from the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina is the first cytochrome c4 to be crystallized from an anaerobic organism. It was crystallized using the addition of metal-ion salts to the standard vapour-diffusion method. Coloured well shaped three-dimensional crystals with dimensions of approximately 0.6 x 0.05 x 0.02 mm grew within 3-4 d at pH 5 and diffracted to 1.72 angstroms without radiation damage. Cytochrome c4 crystallized in space group P4(1)2(1)2 as a primitive tetragonal system with unit-cell parameters a = b = 75.29, c = 37.12 angstroms, alpha = beta = gamma = 90 degrees.
Tomcová
Ivana
I
Institute of Physical Biology, University of South Bohemia in Ceské Budejovice, Zámek 136, CZ-373 33 Nové Hrady, Czech Republic. tomcova@ufb.jcu.cz
Branca
Rui Miguel Mamede
RM
Bodó
Gabriella
G
Bagyinka
Csaba
C
Smatanová
Ivana Kutá
IK
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D003460:Crystallization; D003574:Cytochrome c Group / Q000737:chemistry* / Q000302:isolation & purification; D020586:Thiocapsa / Q000201:enzymology*; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D003574:Cytochrome c Group; C034186:cytochrome C4