title: Protective activity of the antilipopolysaccharide antibodies from human cord serum.
abstract: We evaluated the ability of human maternal and cord serum antibodies to protect mice challenged with live Escherichia coli serotype O6:K2ac (E. coli O6). Mice received paired maternal or cord serum pools before a challenge with E. coli O6 to evaluate the mortality rate. All the pools were able to protect the animals challenged with bacteria except the test group from paired maternal and cord sera from preterm neonates containing less than 1.0 mg L(-1) immunoglobulin G antibody levels. In liver, spleen and mesenteric lymph nodes from the control group (phosphate-buffered saline), more than 10(2) CFU mL(-1) bacteria were found at 30 min and more than 10(5) CFU mL(-1) after 120 min. The test group showed lower bacterial counts in the organs, and no bacteria in the mesenteric lymph nodes during the evaluated period. Tumor necrosis factor alpha and interleukin 6 were undetectable in serum from animals pretreated with paired maternal and cord serum pools from full-term neonates and pools from preterm neonates containing high antibody and avidity levels. Our findings suggest that placental transfer of antilipopolysaccharide O6 immunoglobulin G antibodies to neonates has a high capacity to prevent lethal infection with E. coli O6 in a mouse protection model and that the degree of protection is determined by the concentration and avidity of these IgG antibodies.
authors.affiliation: Department of Immunology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, SP, Brazil. gepontes@usp.br
authors.forename: Gerlândia Neres
authors.initials: GN
authors.lastname: Pontes
authors.forename: Silvia Gomes
authors.initials: SG
authors.lastname: Massironi
authors.forename: Christina
authors.initials: C
authors.lastname: Arslanian
authors.forename: Dora Lisa
authors.initials: DL
authors.lastname: Friedlander-Del Nero
authors.forename: Magda Maria Sales
authors.initials: MM
authors.lastname: Carneiro-Sampaio
authors.forename: Aparecida Tiemi
authors.initials: AT
authors.lastname: Nagao
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000915:Antibody Affinity; D018988:Bacterial Translocation; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000254:growth & development; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D051379:Mice; D010529:Peritoneal Cavity / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D000907:Antibodies, Bacterial; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha