Fluorophore-quencher distance correlation functions from single-molecule photon arrival trajectories.
We generalize and simplify the method of Yang and Xie (J. Chem. Phys. 2002, 117, 10965) to obtain distance correlation functions from photon arrival trajectories of single fluorophores whose lifetime, [k(r)](-1), depends on the distance to a quencher. It is assumed that this distance does not change during the fluorescence lifetime. The experimental trajectory is first transformed by replacing the delay time (i.e., the interval between the photon arrival and the nearest laser pulse) by a certain function of this delay time. This function is the inverse Laplace transform of r(k), which is the solution of k(r) = 1. The correlation function of the transformed data then directly gives the distance correlation function. Illustrative examples include Förster energy transfer and quenching due to electron transfer.
Gopich
Irina
I
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Szabo
Attila
A
D004058:Diffusion; D004583:Electrons; D004735:Energy Transfer; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D015233:Models, Statistical; D008968:Molecular Conformation; D017785:Photons; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D005456:Fluorescent Dyes