Efficacy of planar chromatography coupled to (tandem) mass spectrometry for employment in trace analysis.
HPTLC/MS by a plunger-based extraction device was shown to be an appropriate technique for quantitative planar chromatography, even in trace analysis. Reproducible extraction from silica gel phases in the lower-pg range distinguishes this technique from other approaches. Repeatability of the MS signal showed a mean RSD of 12.5% for the example of Harman, a heterocyclic aromatic amine. Analytical response within a plate and over various plates/days showed determination coefficients of 0.9915 and 0.9488, respectively. Limit of detection (LOD) by a single quadrupole was better than 40 pg and limit of quantitation (LOQ) by a tandem mass spectrometer better than 20 pg. LOQ/LOD obtained were of similar magnitude as reported for HPLC/MS methods, however, this order of sensitivity was shown for the first time in the field of HPTLC/electrospray ionization MS.
Jautz
Ute
U
University of Hohenheim, Institute of Food Chemistry, Garbenstrasse 28, D-70599 Stuttgart, Germany.
Morlock
Gertrud
G
D002855:Chromatography, Thin Layer / Q000295:instrumentation* / Q000379:methods; D005504:Food Analysis / Q000379:methods; D006247:Harmine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D009153:Mutagens / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D009153:Mutagens; D006247:Harmine; D058428:Silica Gel; D012822:Silicon Dioxide; C005010:harman