title: The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants.
abstract: Considering that exogenously applied methyl jasmonate can enhance secondary metabolite production in a variety of plant species and that 2,3-oxidosqualene is a common precursor of triterpenes and sterols in plants, we have studied Centella asiatica and Galphimia glauca (both synthesizing triterpenoid secondary compounds) and Ruscus aculeatus (which synthesizes steroidal secondary compounds) for their growth rate and content of free sterols and respective secondary compounds, after culturing with or without 100 microM methyl jasmonate. Our results show that elicited plantlets of G. glauca and to a higher degree C. asiatica (up to 152-times more) increased their content of triterpenoids directly synthesized from 2,3-oxidosqualene (ursane saponins and nor-seco-friedelane galphimines, respectively) at the same time as growth decreased. In contrast, the free sterol content of C. asiatica decreased notably, and remained practically unaltered in G. glauca. However, in the case of R. aculeatus, which synthesizes steroidal saponins (mainly spirostane type) indirectly from 2,3-oxidosqualene after the latter is converted to the plant phytosterol-precursor cycloartenol, while the growth rate and free sterol content clearly decreased, the spirostane saponine content was virtually unchanged (aerial part) or somewhat lower (roots) in presence of the same elicitor concentration. Our results suggest that while methyl jasmonate may be used as an inducer of enzymes involved in the triterpenoid synthesis downstream from 2,3-oxidosqualene in both C. asiatica and G. glauca plantlets, in those of C. asiatica and R. aculeatus it inhibited the enzymes involved in sterol synthesis downstream from cycloartenol.
authors.affiliation: Laboratorio de Fisiología vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028 Barcelona, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Mangas
authors.forename: Mercè
authors.initials: M
authors.lastname: Bonfill
authors.forename: Lidia
authors.initials: L
authors.lastname: Osuna
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Moyano
authors.forename: Jaime
authors.initials: J
authors.lastname: Tortoriello
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Cusido
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Piñol
authors.forename: Javier
authors.initials: J
authors.lastname: Palazón
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D028041:Centella / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology*; D031582:Galphimia / Q000187:drug effects / Q000378:metabolism*; D015394:Molecular Structure; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology; D031443:Ruscus / Q000187:drug effects / Q000378:metabolism*; D013261:Sterols / Q000737:chemistry / Q000378:metabolism*; D014315:Triterpenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D013261:Sterols; D014315:Triterpenes; C072239:methyl jasmonate