[The application of PCR-SSCP in forensic mtDNA typing].
OBJECTIVE
To study the application of PCR-SSCP in forensic mtDNA typing.


METHODS
Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed.


RESULTS
In 70 family trios, the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in 98.57% and 97.13% respectively but were identical with their mothers. In 140 unrelated Wuhan Han individuals, 21 haplotypes were found in HVI, GD = 0.9556; 16 haplotypes were found in HVII, GD = 0.9356.


CONCLUSIONS
PCR-SSCP technique may be useful in forensic mtDNA typing, especially for screening the suspects.
Ai
Hong-Wei
HW
Department of Forensic Medicine, Huazhong University of Science and Technology, Wuhan 430030, China. aimingxu03@163.com
Yu
Chun-Ying
CY
Zhao
Gui-Sen
GS
Huang
Dai-Xin
DX
Ke
Ji
J
Yue
Xia
X
Yang
Qing-En
QE
D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000097:blood / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods; D006239:Haplotypes; D006801:Humans; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004272:DNA, Mitochondrial