[Effects of T cell receptor gene rearrangement on BV CDR3 in Jurkat cells].
OBJECTIVE
To investigate the effects of T cell receptor (TCR) BD2-BJ2 gene rearrangement on the complementary-determining region (CDR) 3 of TCR beta chain (TCR BV CDR3) in Jurkat cells.


METHODS
TCR BV gene subfamilies were detected by RT-PCR in Jurkat cells during proliferation and after induction with non-specific T cell activators and SEA, respectively. To determine the clonality of TCR BV subfamilies and the lengths of CDR3, the PCR products were analyzed by TCR GeneScan technique, and the sequences of CDR3 were further analyzed by DNA sequencer.


RESULTS
No new TCR BV subfamilies were found in Jurkat cells, a monoclonal BV8(+)cell line, either during cell proliferation or after stimulation with different treatments, nor were any differences found in CDR3 size or sequences.


CONCLUSIONS
TCR BD2-BJ2 rearrangement in Jurkat cells may not play a role in modification of TCR BV CDR3 domains or the consequent antigen immunorecognition of BV CDR3, but the possibility of TCR modification can not be excluded.
Zou
Hong-Yun
HY
Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
Ma
Li
L
Yao
Xin-sheng
XS
Wen
Qian
Q
Luo
Wei
W
Wang
Xiao-Ning
XN
D000595:Amino Acid Sequence; D001483:Base Sequence; D022801:Complementarity Determining Regions / Q000235:genetics*; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics*; D006801:Humans; D007155:Immunologic Factors / Q000235:genetics; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
D022801:Complementarity Determining Regions; D007155:Immunologic Factors; D011948:Receptors, Antigen, T-Cell