[Validation studies of the test strip of colloidal gold-labeled anti-fibronectin EIIIA splicing variant for estimating the time of injury].
OBJECTIVE
To evaluate the validation of the test strip of colloidal gold-labeled anti-Fibronectin EIIIA(Fn-EIIIA) Splicing Variant for estimating the time of injury.


METHODS
After skin tissues with variant injury times were collected from corpses, the test strip were used to detect the expression of Fn-EIIIA and its tested results were valued; The arisen autolysis which may influence the results of test were also observed by rats models.


RESULTS
In no-injured skins, no Fn-EIIIA were detected by the strip, while in all the injured skins it was detected successfully as soon as injured after 1h and it showed more obvious results with the elongating of injured time; No changes made by autolysis were found in our studied time ranges.


CONCLUSIONS
The test strip which used in this study showed to be a sensitive and steady method for estimating the time of injury in forensic science.
Zhang
Jian-Hua
JH
Institute of Forensic Science, Ministry of Justice, P R China. zjh7693@hotmail.com
Liu
Ning-Guo
NG
Chen
Yi-Jiu
YJ
Huang
Xiao-Hua
XH
D017398:Alternative Splicing; D000818:Animals; D001329:Autolysis; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D013997:Time Factors
D005353:Fibronectins; D007202:Indicators and Reagents