Single amino acid substitutions alter the efficiency of docking in modular polyketide biosynthesis.
Weissman
Kira J
KJ
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK. kjw21@cam.ac.uk
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D007783:Lactones / Q000378:metabolism; D018942:Macrolides / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary / Q000235:genetics; D016930:Saccharopolyspora / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
D007783:Lactones; D018942:Macrolides; D009097:Multienzyme Complexes; D048630:Polyketide Synthases