Sensitive HPLC analysis of basic drugs of abuse having weak UV absorptivity using columns with low carbon loadings monitored at 205 nm.
The selection of columns containing cyanopropyl-bonded silica (3.5% carbon loading) and their use under reversed-phase conditions has permitted short retention times with good resolution to be obtained for 16 basic drugs of abuse and three metabolites. Predominantly aqueous mobile phases enable the determination of these weakly UV-absorbing compounds over the concentration range 0.7-18 microg ml(-1) with detection at 205 nm. Trimethylsilyl-bonded silica (2.6% carbon loading) also provided good resolution but required longer analysis times.
Badiru
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School of Pharmacy and Pharmacology, University of Bath, Bath, BA2 7AY, UK.
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