Resonance Raman evidence of immobilization of laccase on self-assembled monolayers of thiols on Ag and Au surfaces.
Resonance Raman (RR) and surface-enhanced Raman scattering (SERS) spectroscopy have been used to study immobilization of laccase on self-assembled monolayers (SAMs) of thiols containing carboxylic and amino groups, deposited on silver and gold electrodes. A new, indirect way of monitoring laccase bound to the thiol-coated Ag and Au surfaces is presented. It was demonstrated that by recording the resonance Raman spectra of the colored product of the oxidation of syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehydeazine) by laccase in the presence of molecular oxygen, one may easily confirm binding as well as enzymatic activity of laccase immobilized on the SAMs modified silver and gold surfaces.
Michota-Kaminska
A
A
Department of Chemistry, University of Warsaw, Pasteur 1, 02-093 Warszawa, Poland.
Wrzosek
B
B
Bukowska
J
J
D015097:3-Mercaptopropionic Acid; D015374:Biosensing Techniques; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D004800:Enzymes, Immobilized; D013438:Sulfhydryl Compounds; D012834:Silver; D006046:Gold; D015097:3-Mercaptopropionic Acid; D042845:Laccase; D010100:Oxygen