title: Detection of Candida dubliniensis in patients with candidiasis in Caracas, Venezuela.
abstract: Candida species are responsible for 80% of all nosocomial fungal infections. In 1995 a new yeast species was described, Candida dubliniensis which shares with Candida albicans characteristics. We have studied 109 yeast isolates identified as C. albicans to investigate the presence of C. dubliniensis by microbiological studies and PCR using DUBR/DUBF primers. Positive results using microbiological tools were between 90 and 98%. Two morphological and physiological of the 80 DNA examined samples (2.5%) showed a PCR product of 288 bp which allow the identification of C. dubliniensis. This is the first report in Venezuela of identification of this species using a PCR approach.
authors.affiliation: Escuela de Medicina José María Vargas, Universidad Central de Venezuela, Caracas, Venezuela.
authors.forename: Ana
authors.initials: A
authors.lastname: Brito Gamboa
authors.forename: Mireya
authors.initials: M
authors.lastname: Mendoza
authors.forename: Alexis
authors.initials: A
authors.lastname: Fernández
authors.forename: Elvia
authors.initials: E
authors.lastname: Díaz
mesh_terms: D002175:Candida / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D002176:Candida albicans / Q000235:genetics; D002177:Candidiasis / Q000453:epidemiology / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis; D005656:Fungal Proteins / Q000302:isolation & purification; D006801:Humans; D009172:Mycology / Q000379:methods; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014687:Venezuela / Q000453:epidemiology; D001617:beta-Glucosidase / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D001617:beta-Glucosidase