title: Crystallization and preliminary X-ray crystallographic studies of transportin 1 in complex with nucleocytoplasmic shuttling and nuclear localization fragments.
abstract: Nucleocytoplasmic transport of proteins with molar masses of larger than 60,000 is mediated by transport receptors. The transport receptor transportin1 (Trn1) transports various kinds of RNA-binding proteins such as JKTBP, hnRNP D and TAP. Trn1 was successfully cocrystallized with nucleocytoplasmic shuttling fragments of JKTBP and hnRNP D and a nuclear localization fragment of TAP. The crystal of the Trn1-JKTBP fragment complex belongs to space group P2(1)2(1)2, with unit-cell parameters a = 131.5, b =171.5, c = 68.2 angstroms. The crystals of Trn1 in complex with hnRNP D and TAP fragments are orthorhombic, space group P2(1)2(1)2(1), with unit-cell parameters a = 69.1, b = 119.1, c = 151.1 angstroms and a = 69.0, b = 119.1, c = 146.0 angstroms, respectively. The crystals diffracted to beyond 3.0, 3.2 and 2.4 angstroms resolution, respectively, using synchrotron radiation at SPring-8.
authors.affiliation: Field of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Imasaki
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hidaka
authors.forename: Michiyuki
authors.initials: M
authors.lastname: Yamada
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sato
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D028884:Karyopherins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000737:chemistry; D017356:Synchrotrons
chemical_list: D028884:Karyopherins; D010446:Peptide Fragments; D016601:RNA-Binding Proteins