title: Enhanced separation of purine and pyrimidine bases using carboxylic multiwalled carbon nanotubes as additive in capillary zone electrophoresis.
abstract: This paper describes the enhanced separation of adenine (A), hypoxanthine (HX), 8-azaadenine (8-AA), thymine (T), cytosine (C), uracil (U) and guanine (G) by CZE dispersing carboxylic multiwalled carbon nanotubes (c-MWNTs) into the running buffer. The effect of important factors such as c-MWNT nanoparticle concentration, the acidity and concentration of running buffer, and separation voltage were investigated to acquire the optimum conditions. The seven purine and pyrimidine bases could be well separated within 16 min in a 35 cm effective length fused-silica capillary at a separation voltage of +8.0 kV in a 23 mM tetraborate buffer (pH 9.2) containing 8.0 x 10(-5) g/mL c-MWNTs. Under the optimal conditions, the linear ranges were of 2-250 microg/mL for A (R2 = 0.995), 3-200 microg/mL for U (R2 = 0.990) and G (R2 = 0.992), 3-250 microg/mL for T (R2 = 0.998), 2-200 microg/mL for C (R2 = 0.985) and 4-200 microg/mL for HX (R2 = 0.988) and 8-AA (R2 = 0.990). The detection limits were 0.9 microg/mL for A (S/N = 3), 2.4 microg/mL for U, 2.0 microg/mL for T, 1.5 microg/mL for C, 2.5 microg/mL for G and 3.0 microg/mL for HX and 8-AA. The proposed method was successfully applied for determining five purine and pyrimidine bases in yeast RNA.
authors.affiliation: Department of Chemistry, Beijing Normal University, Beijing, P R China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xiong
authors.forename: Jin
authors.initials: J
authors.lastname: Ouyang
authors.forename: Willy R G
authors.initials: WR
authors.lastname: Baeyens
authors.forename: Joris R
authors.initials: JR
authors.lastname: Delanghe
authors.forename: Xiumin
authors.initials: X
authors.lastname: Shen
authors.forename: Yiping
authors.initials: Y
authors.lastname: Yang
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011687:Purines / Q000302:isolation & purification*; D011743:Pyrimidines / Q000302:isolation & purification*
chemical_list: D037742:Nanotubes, Carbon; D011687:Purines; D011743:Pyrimidines; C030986:pyrimidine; C030985:purine