title: Proteomics-based identification of autoantibodies in the sera of healthy Chinese individuals from Beijing.
abstract: The identification of panels of tumor antigens that elicit an antibody response may have utility in cancer screening, diagnosis and in establishing prognosis. However, autoantibodies normally exist in sera of healthy individuals and are enormously diversified. To explore the reservoir of autoantibody in healthy population, we performed a proteomics investigation of autoantibody profiles in the sera of 36 healthy Chinese individuals from Beijing, which may provide valuable reference information to the identification of disease-specific autoantibodies. The results showed that autoantibody profiles varied individually, but some autoantibodies were identified at a high frequency in the healthy population. The autoantibodies against alpha-enolase and those against heterogeneous nuclear ribonucleoprotein L were positive in more than 50% of the sera samples. The autoantibodies identified in more than 20% of samples included those against annexin II, F-actin capping protein beta subunit and calreticulin. Some of these autoantibodies have been previously reported to be involved in autoimmune conditions and cancers. Autoantibodies in the healthy population are important as a foundation from which disease-specific autoantibodies can be defined. Thus our report on autoantibodies in healthy individuals may be useful as a reference for defining new autoantibody biomarkers.
authors.affiliation: Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Hui-Yan
authors.initials: HY
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Li
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: He
authors.forename: Bing
authors.initials: B
authors.lastname: Liang
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Bei-Fen
authors.initials: BF
authors.lastname: Shen
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology; D002460:Cell Line; D002681:China; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006262:Health; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods*
chemical_list: D001323:Autoantibodies; D001324:Autoantigens