PRINS as an efficient tool for aneuploidy assessment in human oocytes and preimplantation embryos.
An ultrarapid three- and four-color primed in situ (PRINS) procedure has been developed for rapid chromosome identification and aneuploidy assessment on isolated cells. Based on the direct in situ mixing of fluorochromes (fluorescein isothiocyanate, tetramethylrhodamine isothiocyanate, Cascade Blue), this multicolor PRINS procedure is described on unfertilized human oocytes and isolated human blastomeres.
Pellestor
Franck
F
Institute of Human Genetics, CNRS UPR 1142, Montpellier, France.
Andréo
Brigitte
B
Anahory
Tal
T
Hamamah
Samir
S
D000782:Aneuploidy; D001755:Blastocyst / Q000378:metabolism*; D001757:Blastomeres / Q000378:metabolism; D002877:Chromosomes, Human / Q000235:genetics; D005260:Female; D006801:Humans; D009865:Oocytes / Q000378:metabolism*; D020401:Primed In Situ Labeling / Q000379:methods*