[Purification and viral inactivation of hemoglobin from human placenta blood].
This study was intended to establish a method for preparation of purified and viral-inactivated placenta hemoglobin. The optimum preparative condition resulted in the up-grading of purity,recovery and so on. A quality control was also established for the purification of hemoglobin. Compared to present purification methods, this method is easy to operate, needs low investment and running cost, and has the advantage of simultaneous operation for purification and viral-inactivation. The resulted hemoglobin had high purity and recovery, and the physicochemical property measured up to that in international reports. So this method is suitable for preparing purified and viral-inactivated hemoglobin on an adequate scale, and is useful for further development of blood substitutes.
Li
Tao
T
Department of Biopharmaceutics, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Zhang
Honghui
H
Wang
Hong
H
Yu
Rong
R
Yang
Chengmin
C
D001802:Blood Substitutes; D005312:Fetal Blood / Q000737:chemistry* / Q000821:virology; D006454:Hemoglobins / Q000302:isolation & purification*; D006801:Humans; D038301:Virus Inactivation
D001802:Blood Substitutes; D006454:Hemoglobins