Determination of amiodarone and desethylamiodarone in plasma with a standardised extraction and chromatographic optimisation procedure.
A previously described optimisation procedure was tested by developing suitable HPLC systems for the determination of amiodarone and desethylamiodarone in plasma. Chromatography is performed on a cyano-column (CN-column) in the reversed-phase mode. The optimisation strategy consists of carrying out a gradient elution from which an appropriate solvent strength for isocratic elution is determined. Binary and ternary mobile phase compositions with the same solvent strength but with different solvent selectivity are then used to evaluate selectivity. The anti-arrhythmic drugs are extracted from plasma using an ion-pair extraction procedure with sodium-n-octylsulphate as ion-pairing agent.
De Smet
M
M
Vrije Universiteit Brussel, Farmaceutisch Instituut, Laarbeeklaan 103, B-1090 Brussel, Belgium.
Massart
D L
DL