Analysis of prominent flavonoid aglycones by high-performance liquid chromatography using a monolithic type column.
A fast RP-HPLC method for the simultaneous separation of eleven main flavonoid aglycones in food samples was developed. Flavonoids were divided in three groups according to their hydrophobicity and were resolved by a ternary gradient elution and diode array detection within analysis times less than 15 min. The detection limits of the flavonoids studied were in the range between 0.04 and 0.31 microg/mL, with repeatabilities values between 0.4 and 5%. The method was successfully applied to the analysis of these compounds in complex natural samples, like propolis and Ginkgo biloba.
Repollés
Carme
C
Departament de Química Analítica, Universitat de Barcelona, Diagonal 647, 08028 Barcelona, Spain.
Herrero-Martínez
José Manuel
JM
Ràfols
Clara
C
D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005504:Food Analysis; D020441:Ginkgo biloba / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results
D000097:Acetonitriles; D005419:Flavonoids; D000432:Methanol; C032159:acetonitrile