title: Partial two-dimensional gel electrophoresis (2-DE) maps of Streptococcus iniae ATCC29178 and Lactococcus garvieae KG9408.
abstract: To examine the proteomes of 2 important causative agents of fish streptococcosis, Streptococcus iniae ATCC29178 and Lactococcus garvieae KG9408, we used 2-dimensional gel electrophoresis (2-DE) followed by mass spectrometry to generate 2-DE maps of these type strains. Silver-stained 2-DE gels of S. iniae ATCC29178 and L. garvieae KG9408 revealed approximately 320 and 300 spots, respectively, and immobilized pH gradient strips (13 cm, pH 4 to 7) revealed that the majority of the detected spots were concentrated in the pH range of 4.5 to 5.5. The spots were randomly selected from the 2-DE profiles and identified by peptide mass fingerprinting using matrix-assisted laser desorption/ionization time of flight mass spectrometry. The majority of the identified proteins were functionally related to energy and carbohydrate metabolism (e.g. enolase ATPase, glyceraldehyde-3-phosphate dehydrogenase) or translation and translocation (e.g. elongation factor G, elongation factor Tu, DNA-directed RNA polymerase alpha chain). These data, along with our partial 2-DE maps of S. iniae ATCC29178 and L. garvieae KG9408, may help suggest antigenic proteins for the development of effective diagnostic tools and vaccines against S. iniae and L. garvieae.
authors.affiliation: Laboratory of Fish & Shellfish Diseases, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, ROK.
authors.forename: Gee-Wook
authors.initials: GW
authors.lastname: Shin
authors.forename: K J
authors.initials: KJ
authors.lastname: Palaksha
authors.forename: Hyang-Hee
authors.initials: HH
authors.lastname: Yang
authors.forename: Yong-Seung
authors.initials: YS
authors.lastname: Shin
authors.forename: Young-Rim
authors.initials: YR
authors.lastname: Kim
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Lee
authors.forename: Myung-Joo
authors.initials: MJ
authors.lastname: Oh
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000145:classification*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005399:Fishes; D017045:Lactococcus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D020543:Proteome / Q000235:genetics*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013291:Streptococcus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D020543:Proteome