Microarrays of heparin oligosaccharides obtained by nitrous acid depolymerization of isolated heparin.
Heparin oligosaccharides derived by nitrous acid depolymerization of heparin have been immobilized on amine-coated glass slides. The formation of a Schiff base creates heparin chips that are a suitable platform for the high-throughput analysis of carbohydrate-protein interactions.
de Paz
Jose L
JL
Laboratory for Organic Chemistry, Swiss Federal Institute of Technology (ETH) Zürich, Wolfgang-Pauli-Str. 10, HCI F315, 8093, Zürich, Switzerland.
Spillmann
Dorothe
D
Seeberger
Peter H
PH
D000990:Antithrombin III / Q000737:chemistry / Q000378:metabolism; D020099:Coated Materials, Biocompatible; D016220:Fibroblast Growth Factor 1 / Q000737:chemistry / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000378:metabolism; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D009608:Nitrous Acid / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
D020099:Coated Materials, Biocompatible; D009844:Oligosaccharides; D011506:Proteins; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D000990:Antithrombin III; D006493:Heparin; D009608:Nitrous Acid