A comparative study of reactive oxygen species in red muscle: pressure effects.
In ectotherms as well as in endotherms, the mitochondrial respiratory chain is the major source of reactive oxygen species (ROS) including the highly reactive, hydroxyl radical (OH*). It is known that the different steps involved in ROS production and the antioxidant systems are comparable in ectotherms and endotherms. But regulatory mechanisms in ROS production are less known especially in fish submitted to environmental changes. Firstly, we performed a ROS muscle metabolism interspecies study, using trout and eels as ectotherms and rats as endotherms, measuring OH* production, superoxide dismutase and catalase activities and in parallel oxygen consumption (MO2). Secondly, we studied the effects of an environmental factor (hydrostatic pressure) on ROS metabolism in both fish species. The results show that, at atmospheric pressure, fish have a higher OH*/MO2 ratio than rats which exhibit a higher superoxide dismutase activity. In fish exposed to hydrostatic pressure, this ratio is reversed so that for the same MO2 value, fish produce less OH* under hydrostatic pressure than at atmospheric pressure.
Amérand
A
A
UHPM-UPCI- EA3879, Faculty of Medicine, CS 93837 29238, Brest 3, France.
Vettier
A
A
Sébert
P
P
Moisan
C
C
D000818:Animals; D002374:Catalase / Q000378:metabolism; D004524:Eels; D006874:Hydrostatic Pressure; D017665:Hydroxyl Radical / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism; D014337:Trout
D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; D002374:Catalase; D013482:Superoxide Dismutase