title: Effects of gamma irradiation on BCL2 and TPR53BP2 expression in the porcine ciliary body.
abstract: BACKGROUND
  When dissection of porcine eyes from a living body results in the cessation of aqueous humor production and blood flow, programmed cell death regulated by TPR53BP2 and BCL2 genes may occur in the pigmented epithelium (PE) and non-pigmented epithelium (NPE) of the ciliary body. Blood products are subjected to gamma irradiation in order to prevent cellular damage resulting from transfusion-associated graft-versus-host disease. We investigated whether gamma irradiation influenced BCL2 or TPR53BP2 genes as well as the morphology of the porcine ciliary body.
  
  
  METHODS
  We irradiated the anterior segments of porcine eyes by using (60)Co gamma-rays (20 Gy). To study BCL2 and TPR53BP2 expression, the irradiated specimens were fixed in formalin and embedded in paraffin and then incubated with mouse monoclonal anti-human BCL2 or TPR53BP2 antibody.
  
  
  RESULTS
  Following dissection, an imbalance in homeostasis began with positive BCL2 and TPR53BP2 expression in the edematous ciliary processes, and resulted in atrophy of the NPE. Increased BCL2 and TPR53BP2 expression were evident just after gamma irradiation. Decreased TPR53BP2 expression occurred after 8 h of incubation, and thereby suppressed apoptosis in the NPE; hence, the structure of the ciliary body that was incubated for 8 h after gamma irradiation was well preserved.
  
  
  CONCLUSIONS
  Irradiation renders the ciliary body in enucleated porcine eyes less vulnerable to apoptosis, and thereby exerts a profound preservative effect.
authors.affiliation: Akeo Eye Clinic, 19-5 Ooyamahigashi-cho, Itabashi-ku, Tokyo 173-0014, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Akeo
authors.forename: Tomoo
authors.initials: T
authors.lastname: Funayama
authors.forename: Akira
authors.initials: A
authors.lastname: Ogawa
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamada
authors.forename: Yoko
authors.initials: Y
authors.lastname: Akeo
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D016923:Cell Death / Q000235:genetics; D002924:Ciliary Body / Q000473:pathology / Q000528:radiation effects*; D004848:Epithelium / Q000528:radiation effects; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects; D019254:Genes, bcl-2 / Q000528:radiation effects*; D066298:In Vitro Techniques; D009926:Organ Preservation / Q000379:methods; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D013552:Swine
chemical_list: D011518:Proto-Oncogene Proteins