title: Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells.
abstract: Amphiphilic macromolecules containing a polystyrene-adherent peptide domain and a cell-repellent poly(ethylene glycol) domain were designed, synthesized, and evaluated as a cytophobic surface coating. Such cytophobic, or cell-repellent, coatings are of interest for varied medical and biotechnological applications. The composition of the polystyrene binding peptide domain was identified using an M13 phage display library. ELISA and atomic force spectroscopy were used to evaluate the binding affinity of the amphiphile peptide domain to polystyrene. When coated onto polystyrene, the amphiphile reduced cell adhesion of two distinct mammalian cell lines and pathogenic Staphylococcus aureus strains.
authors.affiliation: Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA. kenan001@mc.duke.edu
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Kenan
authors.forename: Elisabeth B
authors.initials: EB
authors.lastname: Walsh
authors.forename: Steven R
authors.initials: SR
authors.lastname: Meyers
authors.forename: George A
authors.initials: GA
authors.lastname: O'Toole
authors.forename: Erin G
authors.initials: EG
authors.lastname: Carruthers
authors.forename: Woo K
authors.initials: WK
authors.lastname: Lee
authors.forename: Stefan
authors.initials: S
authors.lastname: Zauscher
authors.forename: Carla A H
authors.initials: CA
authors.lastname: Prata
authors.forename: Mark W
authors.initials: MW
authors.lastname: Grinstaff
mesh_terms: D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000502:physiology
chemical_list: D010455:Peptides; D011092:Polyethylene Glycols