Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae.
We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.
Oba
Takahiro
T
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume, Fukuoka 839-0861, Japan. ooba@fitc.pref.fukuoka.jp
Yamamoto
Yoshitsugu
Y
Nomiyama
Shuji
S
Suenaga
Hikaru
H
Muta
Shigeru
S
Tashiro
Kosuke
K
Kuhara
Satoru
S
D015079:2-Isopropylmalate Synthase / Q000235:genetics / Q000378:metabolism*; D025141:Drug Resistance, Fungal; D007930:Leucine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D000439:Pentanols / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D000439:Pentanols; D029701:Saccharomyces cerevisiae Proteins; C035177:5',5',5'-trifluoroleucine; C029683:isopentyl alcohol; D015079:2-Isopropylmalate Synthase; C080062:LEU4 protein, S cerevisiae; D007930:Leucine