Escherichia coli transcriptome dynamics during the transition from anaerobic to aerobic conditions.
Escherichia coli is a metabolically versatile bacterium that is able to grow in the presence and absence of oxygen. Several previous transcript-profiling experiments have compared separate anaerobic and aerobic cultures. Here the process of adaptation was investigated by determining changes in transcript profiles when anaerobic steady-state cultures were perturbed by the introduction of air. Within 5 min of culture aeration the abundances of transcripts associated with anaerobic metabolism were decreased, whereas transcripts associated with aerobic metabolism were increased. In addition to the rapid switch to aerobic central metabolism, transcript profiling, supported by experiments with relevant mutants, revealed transient changes suggesting that the peroxide stress response, methionine biosynthesis, and degradation of putrescine play important roles during the adaptation to aerobic conditions.
Partridge
Jonathan D
JD
Department of Molecular Biology and Biotechnology, the University of Sheffield, Sheffield S10 2TN, United Kingdom.
Scott
Colin
C
Tang
Yue
Y
Poole
Robert K
RK
Green
Jeffrey
J
D000222:Adaptation, Physiological; D000332:Aerobiosis; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000502:physiology; D004268:DNA-Binding Proteins / Q000502:physiology; D004579:Electron Transport; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism / Q000502:physiology; D005285:Fermentation; D020869:Gene Expression Profiling; D008715:Methionine / Q000096:biosynthesis; D010100:Oxygen / Q000494:pharmacology*; D012097:Repressor Proteins / Q000502:physiology; D015534:Trans-Activators / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C499695:IscR protein, E coli; C058169:MetR protein, Bacteria; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C499299:oxyR protein, E coli; D008715:Methionine; D010100:Oxygen