[Qualitative and quantitative determination of the main components of huanglianjiedu decoction by HPLC-UV/MS].
OBJECTIVE
To establish a comprehensive HPLC analytical method of Huanglianjiedu decoction.


METHODS
This study was performed by HPLC-UV/MS to identify the chemical constituents of the whole and individual herbs of the "Huanglianjiedu decoction". Zorbax Extend C18 (150 mm x 4. 6 mm ID, 5 microm) column was used; the mobile phase was composed of acetonitrile (A) and water (B, with 0.5% acetic acid) with gradient elution; the flow rate was 1.0 mL x min(-1) and the column temperature was setup at 25 degrees C. The detection wavelength was 254 nm.


RESULTS
The chromatogram of Huanglianjiedu decoction showed 21 main peaks. Peaks 1, 2, 5 and 18 were from Gardenia jasminoides Ellis, Peaks 8, 13, 14, 15, 16, 17, 19 and 21 from Scutellaria baicalensis Georgi. While 10 from Coptis chinensis Franch and 20 from Phellodendron amurense Rupr., Peaks 3, 4, 6, 9, 11 and 12 came from them together. Peak 7 presented in the chromatograms of the herbs except Gardenia jasminoides Ellis. By comparison of the retention time, the on-line UV spectra and MS spectra, 11 peaks were identified as 5 (geniposide), 9 (jatrorrhizine), 10 (coptisine), 11 (palmatine), 12 (berberine), 13 (baicalin), 15 (oroxin A), 17 (wogonoside), 19 (baicalein), 20 (obaculactone), 21 (wogonin), then eight of them were quantified by HPLC-UV.


CONCLUSIONS
The method could represent the characteristics of Huanglianjiedu decoction, and it could be used to evaluate the quality and quantity of Huanglianjiedu decoction. It distinguished between Coptis chinensis Franch and Phellodendron amurense Rupr. by HPLC for the first time.
Sun
Jian
J
School of Pharmacy, Jilin University, Changchun, China. sunjiankw@163.com
Ma
Ji-sheng
JS
Jin
Jin
J
Wang
Huai-sheng
HS
Wen
Qing-hui
QH
Zhang
Hong-gui
HG
Zhou
Qiu-li
QL
D001599:Berberine / Q000031:analogs & derivatives / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D039323:Coptis / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D032062:Gardenia / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D032102:Phellodendron / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D031364:Scutellaria baicalensis / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; C045607:huang-lien-chieh-tu-tang; C055785:jatrorrhizine; D001599:Berberine; C005413:palmatine