Outer membrane protein changes and efflux pump expression together may confer resistance to ertapenem in Enterobacter cloacae.
We investigated ertapenem-susceptible and -resistant extended-spectrum beta-lactamase-producing Enterobacter cloacae isolates obtained from the same patient. Gene transcription of OmpD and OmpF was diminished in the ertapenem-resistant isolate. An efflux pump inhibitor decreased the MICs of ertapenem in the resistant strain, suggesting a potential role of efflux pumps in ertapenem resistance.
Szabó
Dóra
D
Division of Infectious Diseases, University of Pittsburgh Medical Center, PA 15213, USA.
Silveira
Fernanda
F
Hujer
Andrea M
AM
Bonomo
Robert A
RA
Hujer
Kristine M
KM
Marsh
Jane W
JW
Bethel
Christopher R
CR
Doi
Yohei
Y
Deeley
Kathleen
K
Paterson
David L
DL
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D016972:Enterobacter cloacae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D000077727:Ertapenem; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D014158:Transcription, Genetic; D047090:beta-Lactams / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D026901:Membrane Transport Proteins; D047090:beta-Lactams; D000077727:Ertapenem