title: Fine deletion mapping of chromosome 2q21-37 shows three preferentially deleted regions in oral cancer.
abstract: We analysed the loss of heterozygosity (LOH) of long arm of chromosome 2 by using 16 polymorphic microsatellite markers in 39 matched oral normal and cancer tissues, and defined the deletional mapping of the region with putative tumor suppressor genes. LOH was detected at least one location in 33 of 39 (85%) tumor tissues. Frequent deletions were detected at the locations of microsatellite markers, D2S2304 (35%), D2S111 (40%), D2S155 (35%), D2S1327 (29%), D2S164 (29%), D2S125 (68%) and D2S140 (32%). Three preferentially deleted regions at 2q21-24, 2q33-35 and 2q37.3 were observed. Several candidate tumor suppressor genes in these regions such as LRP1B, CASP8, CASP10, BARD1, ILKAP, PPP1R7, and ING5, are located. Further molecular analysis of each gene should be performed to clarify their roles in oral carcinogenesis.
authors.affiliation: Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayamashi 700-8525, Japan.
authors.forename: Beyhan
authors.initials: B
authors.lastname: Cengiz
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gunduz
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagatsuka
authors.forename: Levent
authors.initials: L
authors.lastname: Beder
authors.forename: Esra
authors.initials: E
authors.lastname: Gunduz
authors.forename: Ryo
authors.initials: R
authors.lastname: Tamamura
authors.forename: Naila
authors.initials: N
authors.lastname: Mahmut
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Fukushima
authors.forename: Mahmoud Al Sheikh
authors.initials: MA
authors.lastname: Ali
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Naomoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Shimizu
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagai
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D009062:Mouth Neoplasms / Q000235:genetics*; D009363:Neoplasm Proteins / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism
chemical_list: D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D010749:Phosphoprotein Phosphatases