Pathological circumstances impair the ability of "dark" neurons to undergo spontaneous recovery.
The effects of dehydrating drugs (furosemide, mannitol and glycerine), potassium channel modulators (tetraethylammonium chloride, 5-hydroxydecanoic acid Na salt, minoxidil and pinacidil), sodium channel modulators (veratridine, brevetoxin-9, 5-(N,N-dimethyl)amiloride and benzamil-HCl) and mitochondrial enzyme inhibitors (3-nitropropionic acid, 2,4-dinitrophenol and chloramphenicol) on the fate of electrically produced "dark" hippocampal dentate granule neurons were investigated. All but one (chloramphenicol) of these bioactive reagents substantially retarded the recovery and increased the death rate of such "dark" neurons. As concerns the dehydrating drugs and ion channel modulators, these effects are considered to be consequences of the fact that relatively large volumes (more than half of the original cell volume) of cytoplasmic fluid (water molecules, inorganic ions and metabolites) leave the affected cells through passive pores within a few minutes. The effects of the mitochondrial enzyme inhibitors appear to indicate that restoration of the original cell volume (recovery) demands metabolic (enzyme-mediated) energy. All these features support our previous assumption that the exogenous circumstances existing acutely after the formation of "dark" neurons in neurological diseases decide whether they will recover or die.
Gallyas
Ferenc
F
Department of Neurosurgery, Pécs University, Hungary. ferenc.gallyas.sen@aok.pte.hu
Gasz
Balázs
B
Szigeti
András
A
Mázló
Mária
M
D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D003681:Dehydration / Q000473:pathology; D018891:Dentate Gyrus / Q000473:pathology; D004558:Electric Stimulation / Q000009:adverse effects / Q000379:methods; D004791:Enzyme Inhibitors / Q000009:adverse effects; D049990:Membrane Transport Modulators / Q000009:adverse effects; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D016622:Silver Staining; D013997:Time Factors
D004791:Enzyme Inhibitors; D049990:Membrane Transport Modulators