Calbindin D-28k immunoreactivity increases in the hippocampus after long-term treatment of soy isoflavones in middle-aged ovariectomized and male rats.
This article investigates the long-term effects of soybean isoflavones (ISO) on the changes of calbindin D-28k (CB) immunoreactivity in the hippocampus in middle-aged ovariectomized female rats as well as middle-aged control female and male rats to identify any correlation between calcium and phytoestrogens. In the CA1 region, CB immunoreactivity in the ovariectomized females was similar to that of the control females, whereas CB immunoreactivity in the males was significantly lower than that of the control females. In the dentate gyrus, CB immunoreactivity in the ovariectomized females and males was significantly lower than that of the control females. CB immunoreactivity in all groups was increased dose-dependently after ISO treatment in the CA1 region and dentate gyrus. This result suggests that ISO treatment enhances the expression of CB immunoreactivity in the hippocampus in the middle-aged rats.
Hwang
In Koo
IK
Department of Anatomy College of Medicine, Hallym University, Chunchon, South Korea.
Lee
Yoon-Bok
YB
Yoo
Ki-Yeon
KY
Kang
Tae-Cheon
TC
Lim
Soon Sung
SS
Sohn
Heon-Soo
HS
Kim
Sang Moo
SM
Kim
Woo-Jung
WJ
Shin
Hyun Kyung
HK
Won
Moo Ho
MH
D000367:Age Factors; D000818:Animals; D064026:Calbindins; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects*; D007150:Immunohistochemistry / Q000379:methods; D007529:Isoflavones / Q000008:administration & dosage*; D008297:Male; D010052:Ovariectomy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D030262:Soybean Proteins / Q000008:administration & dosage*
D064026:Calbindins; D007529:Isoflavones; D064030:S100 Calcium Binding Protein G; D030262:Soybean Proteins