title: Age- and dose-dependent effects of an eicosapentaenoic acid-rich oil on cardiovascular risk factors in healthy male subjects.
abstract: Supplementation with fish oils, rich in n-3 polyunsaturated fatty acids, modifies cardiovascular risk factors. However, dose-response relationships are poorly defined and whether similar effects are seen in young and older subjects is not known. This study determined the effect of supplementing the diet of young and older male subjects with different amounts of an eicosapentaenoic acid (EPA)-rich oil. Healthy young (18-42 years) and older (53-70 years) males were randomized to placebo or 1.35, 2.7 or 4.05 g EPA/day for 12 weeks. There was no effect of EPA on blood pressure or on plasma total, LDL or HDL cholesterol. EPA lowered plasma triacylglycerols, with the maximal effect at the lowest dose. Plasma lipoperoxides decreased in all groups. EPA decreased the lag time of copper-induced lipoprotein peroxidation and the ratio of reduced to total glutathione in the older subjects. The highest dose of EPA increased soluble E-selectin in young subjects, while increasing EPA tended to decrease soluble intercellular adhesion molecule 1 in young and older subjects. Young and older males will gain cardiovascular benefit from increased intake of EPA. Young males are unlikely to suffer adverse consequences from high EPA intake, whereas older males may have an increased risk of lipoprotein peroxidation.
authors.affiliation: Department of Preclinical Sciences LITA Vialba, University of Milan, 74 Via G.B. Grassi, Milan 20157, Italy. roberta.cazzola@unimi.it
authors.forename: Roberta
authors.initials: R
authors.lastname: Cazzola
authors.forename: Samantha
authors.initials: S
authors.lastname: Russo-Volpe
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Miles
authors.forename: Dinka
authors.initials: D
authors.lastname: Rees
authors.forename: Tapati
authors.initials: T
authors.lastname: Banerjee
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Roynette
authors.forename: Solenne J
authors.initials: SJ
authors.lastname: Wells
authors.forename: Marie
authors.initials: M
authors.lastname: Goua
authors.forename: Klaus W J
authors.initials: KW
authors.lastname: Wahle
authors.forename: Philip C
authors.initials: PC
authors.lastname: Calder
authors.forename: Benvenuto
authors.initials: B
authors.lastname: Cestaro
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002318:Cardiovascular Diseases / Q000097:blood / Q000517:prevention & control*; D003300:Copper / Q000494:pharmacology; D004311:Double-Blind Method; D019040:E-Selectin / Q000097:blood; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage* / Q000009:adverse effects; D005395:Fish Oils / Q000008:administration & dosage* / Q000009:adverse effects; D005978:Glutathione / Q000097:blood; D019803:Glutathione Disulfide / Q000097:blood; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008054:Lipid Peroxides / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012307:Risk Factors
chemical_list: D019040:E-Selectin; D005395:Fish Oils; D008054:Lipid Peroxides; D008055:Lipids; D018799:Intercellular Adhesion Molecule-1; D003300:Copper; D015118:Eicosapentaenoic Acid; D005978:Glutathione; D019803:Glutathione Disulfide