title: Human Dectin-1 isoform E is a cytoplasmic protein and interacts with RanBPM.
abstract: Human Dectin-1, a type II transmembrane receptor, is alternatively spliced, generating eight isoforms. Of these isoforms, the isoform E (hDectin-1E) is structurally unique, containing a complete C-type lectin-like domain as well as an ITAM-like sequence. So far, little is known about its function. In the present study, we demonstrated that hDectin-1E was not secreted and it mainly resided in the cytoplasm. Using yeast two-hybrid screening, we identified a Ran-binding protein, RanBPM, as an interacting partner of hDectin-1E. GST pull-down assays showed that RanBPM interacted directly with hDectin-1E and the region containing SPRY domain was sufficient for the interaction. The binding of hDectin-1E and RanBPM was further confirmed in vivo by co-immunoprecipitation assay and confocal microscopic analysis. Taken together, our data provide a clue to the understanding of the function about hDectin-1E.
authors.affiliation: State Key Laboratory of Genetic Engineering and Gene Research Center, Shanghai Medical College of Fudan University, Shanghai 200032, PR China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Xie
authors.forename: Maoyun
authors.initials: M
authors.lastname: Sun
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.forename: Weicheng
authors.initials: W
authors.lastname: Liu
authors.forename: Jianhai
authors.initials: J
authors.lastname: Jiang
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Jianxin
authors.initials: J
authors.lastname: Gu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D002460:Cell Line; D003593:Cytoplasm / Q000737:chemistry; D003598:Cytoskeletal Proteins; D006801:Humans; D037181:Lectins, C-Type; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D020033:Protein Isoforms / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D020931:ran GTP-Binding Protein / Q000378:metabolism*; D000099181:Dectin-1
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; D020931:ran GTP-Binding Protein; D000099181:Dectin-1; C116005:Ran binding protein 9