title: Prophylaxis against carcinogenesis in three kinds of unestablished tumor models via IL12-gene-engineered MSCs.
abstract: Mesenchymal stem cells (MSCs) were adenovirally engineered to secrete interleukin-12 (AdIL-12-MSCs) and evaluated for their anticarcinogenesis efficacy against three kinds of unestablished tumor models including B16 melanoma, LLC Lewis lung cancer and HCC hepatoma. Injection of AdIL-12-MSCs into protected mice before tumor inoculation prevented all of 12 mice in B16 preventive groups, 10 out of 12 in LLC lung cancer model and 11 out of 12 mice in HCC hepatoma model from developing tumors, whereas the control groups pre-receiving PBS were validated for 100% carcinogenesis; the tumor formation rates in free-AdIL-12 and vacant MSC groups were unveiled between approximately 83 and 100% even with plentiful angiogenesis and newborn lymphatic vessels, as well as distant metastases. As a novel approach, AdIL-12-MSC has revealed expected preventive effects on carcinogenesis (P<0.01) with low-toxic, broad-spectrum and long-range superiorities. In conclusion, our data indicate that AdIL-12-MSC possess the potential for tropism to preclinical tumor lesions and deprives surviving or hibernating tumor cells, which have escaped from conventional treatments, of revival and recurrence.
authors.affiliation: National Key Laboratory of Biotherapy and Cancer Center, West China Hospital, and West China Medical School, Sichuan University, People's Republic of China.
authors.forename: Xian-cheng
authors.initials: XC
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Yu-quan
authors.initials: YQ
authors.lastname: Wei
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.forename: Yang-sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Ru
authors.initials: R
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
authors.forename: Lian
authors.initials: L
authors.lastname: Wang
authors.forename: Yong-qian
authors.initials: YQ
authors.lastname: Jia
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Zeng
authors.forename: Jin-liang
authors.initials: JL
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Tian
authors.forename: Bing
authors.initials: B
authors.lastname: Kan
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Lin
authors.forename: Song
authors.initials: S
authors.lastname: Lei
authors.forename: Hong-xin
authors.initials: HX
authors.lastname: Deng
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Wen
authors.forename: Yong-qiu
authors.initials: YQ
authors.lastname: Mao
authors.forename: Jiong
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000517:prevention & control; D045744:Cell Line, Tumor; D005818:Genetic Engineering; D018664:Interleukin-12 / Q000235:genetics*; D008113:Liver Neoplasms / Q000517:prevention & control; D008175:Lung Neoplasms / Q000517:prevention & control; D008546:Melanoma, Experimental / Q000517:prevention & control; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice
chemical_list: D018664:Interleukin-12