The prohibitin genes in Arabidopsis thaliana: expression in seeds, hormonal regulation and possible role in cell cycle control during seed germination.
A fragment encoding a partial sequence of a prohibitin (Phb) gene was isolated. The expression of Phb mRNA and protein in seeds of wild type and mutant Arabidopsis thaliana is presented. Phb mRNA is abundant in wild-type seeds; thus, it may have sequence or structural characteristics responsible for this stability. The 3' untranslated region sequence of a Phb gene has interesting features. We found that Arabidopsis Phb does not interact with a retinoblastoma-related protein or E2F in a yeast two-hybrid system, thus suggesting that the plant protein may have not conserved such interaction, described for mammalian Phb. The possible role of Phb in cell cycle regulation during germination is discussed.
De Diego
Juana G
JG
Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, 37007, Salamanca, Spain.
David Rodríguez
F
F
Rodríguez Lorenzo
José Luis
JL
Cervantes
Emilio
E
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000502:physiology; D000091402:Prohibitins; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D012639:Seeds / Q000378:metabolism* / Q000502:physiology
D010937:Plant Growth Regulators; D000091402:Prohibitins; D012097:Repressor Proteins