title: Palladium(II) and platinum(II) organometallic complexes with the model nucleobase anions of thymine, uracil, and cytosine: antitumor activity and interactions with DNA of the platinum compounds.
abstract: Pd(II) and Pt(II) complexes with the anions of the model nucleobases 1-methylthymine (1-MethyH), 1-methyluracil (1-MeuraH), and 1-methylcytosine (1-MecytH) of the types [Pd(dmba)(mu-L)]2 [dmba = N,C-chelating 2-((dimethylamino)methyl)phenyl; L = 1-Methy, 1-Meura or 1-Mecyt] and [M(dmba)(L)(L')] [L = 1-Methy or 1-Meura; L' = PPh(3) (M = Pd or Pt), DMSO (M = Pt)] have been obtained. Palladium complexes of the types [Pd(C6F5)(N-N)(L)] [L = 1-Methy or 1-Meura; N-N = N,N,N',N'-tetramethylethylenediamine (tmeda), 2,2'-bipyridine (bpy), or 4,4'-dimethyl-2,2'-bipyridine (Me2bpy)] and [NBu4][Pd(C6F5)(1-Methy)2(H2O)] have also been prepared. The crystal structures of [Pd(dmba)(mu-1-Methy)]2, [Pd(dmba)(mu-1-Mecyt)]2.2CHCl3, [Pd(dmba)(1-Methy)(PPh3)].3CHCl3, [Pt(dmba)(1-Methy)(PPh3)], [Pd(tmeda)(C6F5)(1-Methy)], and [NBu4][Pd(C6F5)(1-Methy)2(H2O)].H2O have been established by X-ray diffraction. The DNA adduct formation of the new platinum complexes synthesized was followed by circular dichroism and electrophoretic mobility. Atomic force microscopy images of the modifications caused by the platinum complexes on plasmid DNA pBR322 were also obtained. Values of IC50 were also calculated for the new platinum complexes against the tumor cell line HL-60. All the new platinum complexes were more active than cisplatin (up to 20-fold in some cases).
authors.affiliation: Departamento de Química Inorganica, Universidad de Murcia, 30071-Murcia, Spain. jruz@um.es
authors.forename: José
authors.initials: J
authors.lastname: Ruiz
authors.forename: Julia
authors.initials: J
authors.lastname: Lorenzo
authors.forename: Laura
authors.initials: L
authors.lastname: Sanglas
authors.forename: Natalia
authors.initials: N
authors.lastname: Cutillas
authors.forename: Consuelo
authors.initials: C
authors.lastname: Vicente
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Villa
authors.forename: Francesc X
authors.initials: FX
authors.lastname: Avilés
authors.forename: Gregorio
authors.initials: G
authors.lastname: López
authors.forename: Virtudes
authors.initials: V
authors.lastname: Moreno
authors.forename: José
authors.initials: J
authors.lastname: Pérez
authors.forename: Delia
authors.initials: D
authors.lastname: Bautista
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D003596:Cytosine / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000494:pharmacology*; D010165:Palladium / Q000737:chemistry / Q000494:pharmacology*; D013941:Thymine / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D010165:Palladium; D003596:Cytosine; D004247:DNA; D013941:Thymine