The rapid identification of human influenza neuraminidase N1 and N2 subtypes by ELISA.
An ELISA assay was developed to allow the rapid and accurate identification of human influenza A N1 and N2 neuraminidases. Initial testing using a fetuin pre-coating of wells correctly identified 81.7% of the neuraminidase type from a series of human A(H1N1), A(H1N2) and A(H3N2) viruses. This result could be improved to detect the neuraminidase subtype of almost all human influenza A viruses from a large panel of viruses isolated from 2000 to 2005, if the fetuin pre-coating was removed and the viruses were coated directly onto wells. This method is simple, rapid and can be used to screen large numbers of currently circulating human influenza A viruses for their neurraminidase subtype and is a good alternative to RT-PCR.
Barr
I G
IG
WHO Collaborating Centre for Reference and Research on Influenza, 45 Poplar Road, Parkville, Victoria 3052, Australia. Ian.Barr@influenzacentre.org
McCaig
M
M
Durrant
C
C
Shaw
R
R
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000201:enzymology; D053122:Influenza A Virus, H3N2 Subtype / Q000201:enzymology; D009980:Influenza A virus / Q000145:classification / Q000201:enzymology* / Q000276:immunology; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology; D009439:Neuraminidase / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D009439:Neuraminidase