Adult neural stem cells express glucose transporters GLUT1 and GLUT3 and regulate GLUT3 expression.
In the brain, glucose is transported by GLUT1 across the blood-brain barrier and into astrocytes, and by GLUT3 into neurons. In the present study, the expression of GLUT1 and GLUT3 mRNA and protein was determined in adult neural stem cells cultured from the subventricular zone of rats. Both mRNAs and proteins were coexpressed, GLUT1 protein being 5-fold higher than GLUT3. Stress induced by hypoxia and/or hyperglycemia increased the expression of GLUT1 and GLUT3 mRNA and of GLUT3 protein. It is concluded that adult neural stem cells can transport glucose by GLUT1 and GLUT3 and can regulate their glucose transporter densities.
Maurer
Martin H
MH
Department of Physiology and Pathophysiology, University of Heidelberg, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany. maurer@uni-hd.de
Geomor
H Kenny
HK
Bürgers
Heinrich F
HF
Schelshorn
Dominik W
DW
Kuschinsky
Wolfgang
W
D000818:Animals; D015687:Cell Hypoxia; D005786:Gene Expression Regulation; D005947:Glucose / Q000494:pharmacology; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism*; D051273:Glucose Transporter Type 2 / Q000235:genetics / Q000378:metabolism*; D051274:Glucose Transporter Type 3 / Q000235:genetics / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*
D051272:Glucose Transporter Type 1; D051273:Glucose Transporter Type 2; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D012333:RNA, Messenger; D005947:Glucose