Enhancement of serotonin- and bradykinin-evoked calcium ion dynamics in differentiated NG108-15 cells.
Dynamic changes in the concentration of intracellular free-calcium ion ([Ca(2+)](i)) by carbachol (CCh) and neurotransmitter candidates was investigated in undifferentiated and differentiated neuroblastomaxglioma hybrid NG108-15 (NG) cells. [Ca(2+)](i) was increased in a dose-dependent manner by bradykinin (BK) and serotonin (5-HT) in differentiated NG cells, and the response to BK and 5-HT was significantly greater than that in undifferentiated NG cells. The EC(50) value of BK was approximately 1.5 x 10(-8)M in both undifferentiated and differentiated NG cells. The EC(50) value of 5-HT in differentiated NG cells was about 5 x 10(-6)M. The response to BK and 5-HT was almost completely inhibited by 10 nM Hoe140 (a BK B2 receptor antagonist) and 3 nM tropisetron (a 5-HT(3) receptor antagonist), respectively. These results suggest that there are some mechanisms by which the response evoked by BK and 5-HT is up-regulated in differentiated NG cells.
Imanishi
Takashi
T
Department of Biological Chemistry, School of Pharmaceutical Sciences, Kinki University, Kowakae 3-4-1, Higashiosaka 577 8502, Japan.
Matsushima
Kayoko
K
Kawaguchi
Akinori
A
Wada
Tetsuyuki
T
Yoshida
Shigeru
S
Ichida
Seiji
S
D000109:Acetylcholine / Q000494:pharmacology / Q000502:physiology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D005910:Glioma; D018698:Glutamic Acid / Q000494:pharmacology / Q000502:physiology; D006822:Hybrid Cells; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D009447:Neuroblastoma; D018377:Neurotransmitter Agents / Q000494:pharmacology / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology / Q000502:physiology; D051381:Rats; D012701:Serotonin / Q000494:pharmacology / Q000502:physiology*
D002413:Cations, Divalent; D018377:Neurotransmitter Agents; D012701:Serotonin; D018698:Glutamic Acid; D000109:Acetylcholine; D001920:Bradykinin; D002118:Calcium; D009638:Norepinephrine