[PCR-RFLP analysis of the mtDNA Cytb gene in three different horse breeds].
Restriction endonucleases, namely BamH I, Taq I, Hae III, Rsa I and Hinc II, were used to analyze the polymorphism of partial mtDNA Cytb gene sequences from 256 horses 6 types (Thoroughbred, Sanhe, Wuzhumuqin, Xinihe, Wushen and Pony) including the imported breed, cultivated breed and local breed. The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining. Results indicated BamH I and Taq I polymorphism. In all 7 restriction patterns were defected that could be sorted into 3 haplotypes, of which haplotypes I and III were the basic haplotypes. We infer that these horses came from one female ancestor through the analysis thorough one pattern, namely BamH I-B.
Li
Jin-Lian
JL
College of Animal Science and Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China. lijinlian1979@yahoo.com.cn
Shi
You-Fei
YF
Bu
Ren-Qiqige
RQ
Mang
Lai
L
D000818:Animals; D001483:Base Sequence; D001947:Breeding; D045303:Cytochromes b / Q000235:genetics* / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000378:metabolism; D018397:Equidae / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006239:Haplotypes; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004272:DNA, Mitochondrial; D045303:Cytochromes b