Sequence characterization, tissue-specific expression and polymorphism of the porcine (Sus scrofa) liver-type fatty acid binding protein gene.
In this study, the full-length cDNA of porcine liver-type fatty acid binding protein gene (L-FABP) was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequence and the predicted protein sequence share a high sequence identity with their mammalian counterparts. Semi-quantitative RT-PCR revealed that porcine L-FABP gene is expressed in all twelve tissues studied, but a transcript is more abundant in liver and small intestine than in other tissues. The part genomic DNA of the porcine L-FABP gene was amplified by PCR. The coding region of the pig L-FABP gene is organized in four exons and spans an approximate 2.62 kb genomic region. Comparative sequencing of four pig breeds revealed a C-->T single nucleotide polymorphism (SNP) within exon 2. The allele and genotype frequencies differed significantly between indigenous Chinese Zang, Dahe, and Yanan pigs with higher frequencies of allele C and genotype CC and Yorkshire pigs with higher frequencies of allele T and genotype TT (P < 0.01). The association analysis suggested that the C-->T polymorphism was associated with intramuscular fat content, indicating that the SNP is a potential molecular marker for intramuscular fat content.
Jiang
Yan-Zhi
YZ
College of Animal Science and Technology, Sichuan Agricultural University, Ya' an 625014, China. jiangyz04@163.com
Li
Xue-Wei
XW
Yang
Guang-Xi
GX
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D034421:Sus scrofa / Q000235:genetics*
D050556:Fatty Acid-Binding Proteins