Cell-directed assembly of lipid-silica nanostructures providing extended cell viability.
Amphiphilic phospholipids were used to direct the formation of biocompatible, uniform silica nanostructures in the presence of Saccharomyces cerevisiae and bacterial cell lines. The cell surfaces organize multilayered phospholipid vesicles that interface coherently with the silica host and help relieve drying stresses that develop with conventional templates. These host structures maintain cell accessibility, addressability, and viability in the absence of buffer or an external fluidic architecture. The cell surfaces are accessible and can be used to localize added proteins, plasmids, and nanocrystals. Prolonged cell viability combined with reporter protein expression enabled stand-alone cell-based sensing.
Baca
Helen K
HK
Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, NM 87185, USA.
Ashley
Carlee
C
Carnes
Eric
E
Lopez
Deanna
D
Flemming
Jeb
J
Dunphy
Darren
D
Singh
Seema
S
Chen
Zhu
Z
Liu
Nanguo
N
Fan
Hongyou
H
López
Gabriel P
GP
Brozik
Susan M
SM
Werner-Washburne
Margaret
M
Brinker
C Jeffrey
CJ
D001412:Bacillus subtilis / Q000502:physiology*; D002021:Buffers; D002462:Cell Membrane; D018914:Cells, Immobilized; D004926:Escherichia coli / Q000502:physiology*; D036681:Fluorescence Recovery After Photobleaching; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers; D008823:Micelles; D008854:Microscopy, Electron; D049329:Nanostructures; D010743:Phospholipids; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D012542:Scattering, Radiation; D012822:Silicon Dioxide; D014965:X-Rays
D002021:Buffers; D008051:Lipid Bilayers; D008823:Micelles; D010743:Phospholipids; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D012822:Silicon Dioxide