Starch-synthase III family encodes a tandem of three starch-binding domains.
The starch-synthase III (SSIII), with a total of 1025 residues, is one of the enzymes involved in plants starch synthesis. SSIII from Arabidopsis thaliana contains a putative N-terminal transit peptide followed by a 557-amino acid SSIII-specific domain (SSIII-SD) with three internal repeats and a C-terminal catalytic domain of 450 amino acids. Here, using computational characterization techniques, we show that each of the three internal repeats encodes a starch-binding domain (SBD). Although the SSIII from A. thaliana and its close homologous proteins show no detectable sequence similarity with characterized SBD sequences, the amino acid residues known to be involved in starch binding are well conserved.
Palopoli
Nicolás
N
Centro de Estudios e Investigaciones, Universidad Nacional de Quilmes, Bernal, Argentina.
Busi
María Victoria
MV
Fornasari
María Silvina
MS
Gomez-Casati
Diego
D
Ugalde
Rodolfo
R
Parisi
Gustavo
G
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000737:chemistry*; D001483:Base Sequence; D001665:Binding Sites; D005964:Glucosyltransferases / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D020539:Sequence Analysis, Protein
D029681:Arabidopsis Proteins; D005964:Glucosyltransferases; C504530:starch synthase III, Arabidopsis