[Polymorphism distributions of 9 microsatellite Loci in Chinese merino sheep].
The polymorphism distributions of nine microsatellite loci, BM6506, BM1824, BM6438, ILSTS004 and OarDB6 on Chinese merino sheep chromosome 1 and BM4621, OarHH55, BM143 and OarJMP8 in sheep chromosome 6 were determined by polymerase chain reaction (PCR) and multiplex gel electrophoresis followed by silver staining. Gene frequency (Pi), power of discrimination (DP), heterozygosity (H), polymorphism information content (PIC) and probability of paternity exclusion (PE) were calculated. All loci obeyed Hardy-Weinberg equilibrium. BM4621 displayed the highest DP, H, PIC and PE values among the nine microsatellite loci. Cumulative DP of the nine microsatellite loci is 0.99999 and cumulative PE is 0.99915. These results showed that the nine microsatellite loci could be used in linkage analysis, individual identification and paternity test in Chinese merino sheep.
Zhao
Zong-Sheng
ZS
College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China.
Wang
Gen-Lin
GL
Guo
Ji-Gang
JG
Li
Da-Quan
DQ
D000818:Animals; D002681:China; D005260:Female; D005787:Gene Frequency; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D012756:Sheep / Q000235:genetics*