title: Expression and mapping of lubricin in canine flexor tendon.
abstract: Matrix composition and the biomechanical environment are intimately interdependent in most connective tissues. Lubricin has many distinct biological functions, including lubrication, antiadhesion, and cytoprotection in cartilage, tendons, and other tissues. This study investigated the distribution of lubricin in the canine flexor digitorum profundus (FDP) tendon by immunohistochemistry. Lubricin was found both on the tendon surface and at the interface of collagen fiber bundles within the tendon, where the cells are subjected to shear force in addition to tension and compression. The expression of lubricin in regions of the canine flexor tendon that differ in mechanical or nutritional environment was also investigated using RT-PCR. Six N-terminal splicing variants were identified from six distinct anatomical regions of flexor tendon. The variants with larger sizes were noted in regions subjected to significant shear and compressive forces. Lubricin is ubiquitous in the FDP tendon, with variations in distribution and splicing that appear to correspond to discrete anatomic locations that differ by mechanical or nutritional environment.
authors.affiliation: Orthopedic Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Sun
authors.forename: Evelyn J
authors.initials: EJ
authors.lastname: Berger
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Zhao
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Jay
authors.forename: Kai-Nan
authors.initials: KN
authors.lastname: An
authors.forename: Peter C
authors.initials: PC
authors.lastname: Amadio
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002874:Chromosome Mapping; D004285:Dogs; D005091:Exons / Q000235:genetics; D051445:Fibrocartilage / Q000378:metabolism; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D007150:Immunohistochemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013710:Tendons / Q000166:cytology / Q000378:metabolism*; D000099158:Proteoglycan 4
chemical_list: D006023:Glycoproteins; D000099158:Proteoglycan 4