Human and mouse hemoglobin association with the transgenic mouse erythrocyte membrane.
Transgenic mouse models of hemoglobinopathies unravel pathophysiological mechanisms; yet the validity of the red blood cell (RBC) model of human hemoglobin (hHb) enveloped by a mouse (m) membrane has been questioned. Isoelectric focusing of hHb and mHb from transgenic mRBC shows a greater association of mHb to the mouse membrane compared to normal hHbA, supporting a species-specific Hb-mRBC membrane interaction. Enhanced hmutant Hb (HbE, HbS and HbC)-mRBC membrane affinities correlates with enhanced membrane lipid peroxidation and parallel those reported in hRBC, lending support to transgenic mRBC as models of hemoglobinopathies. Species-specific Hb-membrane interaction may be overridden by Hb charge and conformational alterations.
Chen
Qiuying
Q
Department of Medicine, Division of Hematology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Balazs
Tania C
TC
Nagel
Ronald L
RL
Hirsch
Rhoda Elison
RE
D000818:Animals; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006441:Hemoglobin A / Q000378:metabolism; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015227:Lipid Peroxidation; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D013045:Species Specificity
D006454:Hemoglobins; D006441:Hemoglobin A