Automated flow injection determination of salicylates using Trinder reaction for clinical analysis, assays and dissolution studies of formulations.
An automated flow-injection technique is described for salicylate (25-250 or 80-800 mg l(-1)), salicylamide (30-300 mg l(-1)), methylsalicylate (100-1000 mg l(-1)) and acetylsalicylic acid (after alkaline hydrolysis), based on their colour reaction with iron (III) in weak acid medium. The method was evaluated for the determination of salicylate in serum, the assay of the drugs in commercial formulations and automated dissolution studies of drug tablets. There were decreased interferences because of the short reaction time. The precision was good with RSD less than 1% in all cases. Recoveries of salicylate from spiked sera (100-1000 mg l(-1)) varied from 96.4-102.5% (mean 99.3%), and from spiked sample solutions of acetylsalicylic acid, 97.8-103.0% (mean 99.6%). The results of the analysis of commercial drug formulations obtained with the proposed method agreed well with the current USP and BP procedures, with differences of 0.4-1.5% (mean 0.8%). High measurement rates of 180 or 95 per hour were achieved using manifolds without and with predilution respectively.
Koupparis
M A
MA
University of Athens, Department of Chemistry, Laboratory of Analytical Chemistry, 104 Solonos str., Athens 106 80, Greece.
Anagnostopoulou
P I
PI