title: Circulating endothelial progenitor cells are inversely correlated with the median oxygen tension in the tumor tissue of patients with cervical cancer.
abstract: Tumor hypoxia leads to adaptive responses in cancer cells, including an induction of vasculogenesis initiated by circulating endothelial progenitor cells (EPCs) and circulating endothelial cells (CECs). The aim of the present study was to correlate the number of EPCs and CECs with the oxygenation of cervical cancer. Blood concentrations of EPCs were detected by FACS analysis with antibodies for CD34 and vascular endothelial growth factor receptor 2 (VEGFR2). CECs were evaluated by double staining for 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine-labeled acetylated low density lipoprotein (Di-LDL) and lectin in a cell culture assay. Ten patients with cervical cancer were compared with ten healthy volunteers. Intratumoral oxygen tension was assessed polarographically with the computerized Eppendorf histography system. Analysis of CEC numbers revealed no difference between patients and controls. However, patients had lower concentrations of CD34-positive hematopoietic stem cells (HSCs) but a significantly higher fraction of EPCs related to the number of HSCs (1.09% versus 0.53%). This fraction was significantly inversely correlated to the median oxygen tension (r = -0.74, p = 0.015). Our study shows for the first time a significant inverse correlation between the fraction of EPCs and intratumoral oxygen tension. We conclude that the fraction of EPCs should be further evaluated as a useful and convenient marker in the prediction of tumor tissue oxygenation.
authors.affiliation: Department of Hematology/Oncology, University of Leipzig, D-04103 Leipzig, Germany. martin.raida@medizin.uni-leipzig.de
authors.forename: M
authors.initials: M
authors.lastname: Raida
authors.forename: T
authors.initials: T
authors.lastname: Weiss
authors.forename: C
authors.initials: C
authors.lastname: Leo
authors.forename: D
authors.initials: D
authors.lastname: Lenz
authors.forename: A
authors.initials: A
authors.lastname: Tarnok
authors.forename: K
authors.initials: K
authors.lastname: Ameri
authors.forename: A L
authors.initials: AL
authors.lastname: Harris
authors.forename: M
authors.initials: M
authors.lastname: Höckel
authors.forename: D
authors.initials: D
authors.lastname: Niederwieser
mesh_terms: D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D006801:Humans; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
chemical_list: D040301:Vascular Endothelial Growth Factor Receptor-2; D010100:Oxygen