Detection of antimicrobial activities and bacteriocin structural genes in faecal enterococci of wild animals.
The production of antimicrobial activities as well as the presence of bacteriocin structural genes (entA, entB, entP, entQ, cylL, entAS-48, bac31, and entL50A/B) were studied in 140 non-selected faecal enterococcal isolates recovered from wild animals. Eight different indicator strains (including Listeria monocytogenes, Pediococcus pentosaceus, and different enterococcal species) were used for antimicrobial activity detection. Twenty-five of the 140 enterococci (18%) showed antimicrobial activity against L. monocytogenes and 33 additional isolates (24%) showed antimicrobial activity against other indicator strains, but Listeria. At least one bacteriocin structural gene was detected in 17 of the 25 enterococci with antimicrobial activity against L. monocytogenes and different combinations of entA, entB, entP, entQ, entL50A/B, and cylL genes were detected; entA and entB were the most prevalent detected genes, and they were generally associated. Bacteriocin structural genes were detected in 10 of 33 isolates with antimicrobial activity against indicator strains other than Listeria, and the cylL gene was the most prevalent one, especially in E. faecalis isolates.
Poeta
Patricia
P
Universidade de Trás-os-Montes e Alto Douro, Departamento de Ciências Veterinárias, Vila Real, Portugal.
Costa
Daniela
D
Rojo-Bezares
Beatriz
B
Zarazaga
Myriam
M
Klibi
Naouel
N
Rodrigues
Jorge
J
Torres
Carmen
C
D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D001430:Bacteriocins / Q000235:genetics / Q000494:pharmacology*; D016983:Enterococcus / Q000187:drug effects*; D013293:Enterococcus faecalis / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D008089:Listeria monocytogenes / Q000187:drug effects*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
D000890:Anti-Infective Agents; D001430:Bacteriocins; D037521:Virulence Factors