Scopoletine as fluorescence probe for determination of protein.
Scopoletine (SLT), 7-hydroxy-6-methoxylcoumarin, is known to possess biological activities such as abirritating and anti-tumor, it can quench intrinsic fluorescence of bovine serum albumin (BSA) and the fluorescence intensity of itself is enhanced. So, SLT is used as fluorescence probe for quantitative determination of protein. The experiments indicate that under optimum conditions, the enhanced intensity of fluorescence is in proportion to the concentration of proteins in a wide range, and their detection limits (S/N=3) are 1.4 x 10(-8)g mL(-1) for BSA and 1.1 x 10(-8)g mL(-1) for HSA, respectively. Samples were satisfactorily determined. The interaction mechanism is also discussed.
Chen
Yanjing
Y
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
Yang
Jinghe
J
Wang
Zhiling
Z
Wu
Xia
X
Wang
Fei
F
D000818:Animals; D002021:Buffers; D002138:Calibration; D002417:Cattle; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008027:Light; D012542:Scattering, Radiation; D012603:Scopoletin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry
D002021:Buffers; D005456:Fluorescent Dyes; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D012603:Scopoletin