[Coexpression of hepatitis B virus X gene and hepatitis C virus C gene in HepG2 cells and its effect on the expression of VEGF].
OBJECTIVE
To establish an experimental model of HCV C-HBV X co-expression protein and explore its effect on the expression of VEGF.


METHODS
The HBV X gene was recovered by enzyme excision and inserted into PBK-CMV and PBK-HCVC, and recombinant plasmids PBK-X and PBK-X-C were constructed. The plasmids PBK-CMV, PBK-X, PBK-HCVC and PBK-X-C were transfected into HepG2 cells with liposomes. After being selected by G418, resistant colonies were obtained. Reverse transcription PCR and Western blot were used to show HBV X and HCV core protein expression. VEGF was analyzed using immunohistochemical methods and Western blot.


RESULTS
The recombinant plasmid PBK-X-C expressed HBV X and HCV core protein efficiently under the control of the vectors promoter. VEGF and VEGF mRNA of the cells co-expressing HCV C-HBV X proteins were higher than those cells expressing HBV X, HCV C and vector alone.


CONCLUSIONS
HBV X-HCV C co-expression protein can increase the expression of VEGF of HepG2 cells. It suggests that HBV and HCV have a synergic action in the carcinogenesis.
Liu
Chong-yang
CY
Department of Gastroenterology, Daping Hospital, Third Military Medical College, Chongqing 400042, China. chongyangliu@yahoo.com
Liu
Wei-wen
WW
Chen
Dong-feng
DF
Wang
Jun
J
D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D056945:Hep G2 Cells; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D015534:Trans-Activators / Q000235:genetics*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014758:Viral Core Proteins / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins
D015534:Trans-Activators; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D014758:Viral Core Proteins; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; C069130:nucleocapsid protein, Hepatitis C virus