title: Absence of linkage to chromosomes 6q and 16p in a Greek population with knee osteoarthritis.
abstract: Osteoarthritis (OA) is a common age-related debilitating disease of the joints characterized by degeneration of the articular cartilage which leads to joint pain, discomfort, and immobility. Several risk factors have been associated with OA including a genetic predisposition. Specific chromosomal regions have thus far been associated with susceptibility to OA, the strongest being on chromosomes 2, 6, and 16. We hereby report our data on 34 Central Greek knee OA families that were investigated for linkage to the chromosome 6q and 16p susceptibility regions. All affected members had undergone total knee replacement surgery (TKR) at a single large Orthopedics Unit in Central Greece. Nineteen microsatellite markers were selected, 15 for chromosome 6q and 4 for chromosome 16p at a distance of approximately 7 cm. Allele fragment sizes were determined by an automated DNA sequencer using the Fragment Analysis Software. Our results revealed a statistically significant difference in the ratio of affected females to males with knee OA and also showed that there is no evidence of linkage to regions 6q and 16p in a cohort of Central Greek pedigrees with knee OA.
authors.affiliation: Department of Biology, Laboratory of Cytogenetics and Medical Genetics, Medical School, University of Thessaly, 41 222 Larissa, Greece. atsezou@med.uth.gr
authors.forename: Aspasia
authors.initials: A
authors.lastname: Tsezou
authors.forename: Theophilos
authors.initials: T
authors.lastname: Karachalios
authors.forename: Pelagia
authors.initials: P
authors.lastname: Fytili
authors.forename: Eirini
authors.initials: E
authors.lastname: Giannatou
authors.forename: Kyproula
authors.initials: K
authors.lastname: Christodoulou
authors.forename: Georgios M
authors.initials: GM
authors.lastname: Hadjigeorgiou
authors.forename: Konstantinos N
authors.initials: KN
authors.lastname: Malizos
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D015331:Cohort Studies; D004247:DNA / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D006115:Greece; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000209:etiology / Q000235:genetics*; D010375:Pedigree; D044465:White People / Q000235:genetics
chemical_list: D004247:DNA