[Bevacizumab (Avastin) inhibits lung cancer-induced pulmonary microvascular angiogenesis].
OBJECTIVE
To study the effect of bevacizumab (Avastin) on pulmonary microvascular angiogenesis induced by lung cancer.


METHODS
The effects of Avastin on cell growth, cell cycle and apoptosis of lung microvascular endothelial cells were assayed by flow cytometry.


RESULTS
Avastin inhibited the growth of lung microvascular endothelial cells and induced increase of cell apoptosis index in a concentration-dependent manner. With the increment of Avastin concentration from 25 to 100 microg/ml, avascular area increased from 0.944-/+0.073 cm square to 5.189-/+0.192 cm square, and the cell apoptosis index increased from 32.5%-/+1.5% to 39.25-/+1.6%.


CONCLUSIONS
Avastin can inhibit the growth and induce apoptosis of lung microvascular endothelial cells in three-dimensional culture, possibly in association with cell cycle arrest in G(0/1) phase.
Liu
Jin-xin
JX
Cancer Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Wang
Xiao-guang
XG
Fu
Jun
J
Luo
Rong-cheng
RC
D000230:Adenocarcinoma / Q000098:blood supply / Q000473:pathology; D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D061067:Antibodies, Monoclonal, Humanized; D017209:Apoptosis / Q000187:drug effects*; D000068258:Bevacizumab; D002196:Capillaries / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000098:blood supply / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology; D051381:Rats
D020533:Angiogenesis Inhibitors; D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D000068258:Bevacizumab