[Screening of peptide inhibitors of acetylcholinesterase from 12-mer random phage display peptide library].
OBJECTIVE
To screen the peptide inhibitor of acetylcholinesterase (AChE) from 12-mer random phage display peptide library.


METHODS
Human AChE was used as the target to screen its binding peptides from 12-mer random phage display peptide library. The positive phage clones were isolated after three rounds of biopanning followed then by sequence analysis and their activity evaluation.


RESULTS
Six positive phage clones binding to human AChE were obtained, and 4 of them sharing the conservative sequence W(S/P)HY inhibited the enzyme activity of AChE.


CONCLUSIONS
Acquisition of AChE inhibitor from phage display library provides clues for designing peptide inhibitors of AChE.
Zhang
Xing-mei
XM
Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China. shiys@fimmu.com
Shi
Yu-sheng
YS
Wang
Chun-xia
CX
D000110:Acetylcholinesterase / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding
D002800:Cholinesterase Inhibitors; D019151:Peptide Library; D010455:Peptides; D000110:Acetylcholinesterase