title: Activation profile of the F3/Contactin gene in the developing mouse cerebellum.
abstract: In this study, we address the activation profile of the gene encoding the mouse axonal glycoprotein F3/Contactin. Promoter sequences previously characterized in vitro are used to drive an Enhanced Green Fluorescent Protein reporter in transgenic mice. In developing cerebellum, differential transgene expression occurs within distinct cell populations. At P0 the transgene is activated in postmitotic granule neurons undergoing radial migration, a sharp upregulation occurring at P6-P8, with a gradual decline from this stage onward. In Purkinje cells, promoter activation, first detected at P3, peaks at around P6 and is fully downregulated by P16. The transgene is also expressed in Ng2- and O4-positive cells, mostly at the end of the first postnatal week, suggesting correlation with early oligodendrocyte differentiation. These data indicate that the complex organization of the regulatory region of the F3/Contactin gene is necessary for directing its articulated expression in different neural cells types and for its developmental function.
authors.affiliation: Department of Pharmacology and Human Physiology, Medical School, University of Bari, Bari, Italy.
authors.forename: Leonarda
authors.initials: L
authors.lastname: De Benedictis
authors.forename: Antonella
authors.initials: A
authors.lastname: Bizzoca
authors.forename: Patrizia
authors.initials: P
authors.lastname: Corsi
authors.forename: Ilaria
authors.initials: I
authors.lastname: Albieri
authors.forename: G Giacomo
authors.initials: GG
authors.lastname: Consalez
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Gennarini
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D000941:Antigens / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D058966:Contactins; D004789:Enzyme Activation / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000145:classification / Q000378:metabolism*; D019081:O Antigens / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D015816:Cell Adhesion Molecules, Neuronal; D058966:Contactins; D049452:Green Fluorescent Proteins; D019081:O Antigens; D011509:Proteoglycans; D014404:Tubulin; D000099216:Chondroitin Sulfate Proteoglycan 4