Effect of phosphonoformic acid in the development of bovine embryos in vitro.
This research evaluated the ability of phosphonoformic acid to inhibit bovine herpesvirus 1 (BHV-1) in cumulus cells commonly used in co-culture with bovine in vitro-produced embryos. At 200 and 400 microg/ml, phosphonoformic acid inhibited 4 logs of BHV-1. Subsequently, phosphonoformic acid (200 and 400 microg/ml) added to both in vitro fertilization and culture medium resulted in a decrease in the proportion of developed blastocysts, and the number of cells per blastocyst was lower in the treated embryos. Therefore, while phosphonoformic acid can effectively inhibit replication of BHV-1 in co-culture cells, it also inhibits development of in vitro-produced bovine embryos.
Marley
Mylissa S D
MS
Department of Pathobiology, College of Veterinary Medicine, 126 Sugg Laboratory, Auburn University, Auburn, AL 36849, USA.
Givens
M Daniel
MD
Stringfellow
David A
DA
Galik
Patricia K
PK
Riddell
Kay P
KP
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002417:Cattle / Q000196:embryology*; D018920:Coculture Techniques; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000187:drug effects / Q000821:virology*; D047108:Embryonic Development / Q000187:drug effects*; D017245:Foscarnet / Q000494:pharmacology*; D007242:Herpesvirus 1, Bovine / Q000187:drug effects* / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D017245:Foscarnet