Production of tannase from Aspergillus ruber under solid-state fermentation using jamun (Syzygium cumini) leaves.
Tannase producing fungal strains were isolated from different locations including garbages, forests and orchards, etc. The strain giving maximum enzyme yield was identified to be Aspergillus ruber. Enzyme production was studied under solid state fermentation using different tannin rich substrates like ber leaves (Zyzyphus mauritiana), jamun leaves (Syzygium cumini), amla leaves (Phyllanthus emblica) and jawar leaves (Sorghum vulgaris). Jamun leaves were found to be the best substrate for enzyme production under solid-state fermentation (SSF). In SSF with jamun leaves, the maximum production of tannase was found to be at 30 degrees C after 96 h of incubation. Tap water was found to be the best moistening agent, with pH 5.5 in ratio of 1:2 (w/v) with substrate. Addition of carbon and nitrogen sources to the medium did not increase tannase production. Under optimum conditions as standardized here, the enzyme production was 69 U/g dry substrate. This is the first report on production of tannase by A. ruber, giving higher yield under SSF with agro-waste as the substrate.
Kumar
Rakesh
R
Department of Biotechnology, Kurukshetra University, Kurukshetra-136 119, Haryana, India.
Sharma
Jitender
J
Singh
Randhir
R
D001230:Aspergillus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis* / Q000302:isolation & purification; D004783:Environmental Microbiology; D005285:Fermentation; D005618:Fresh Water; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D027842:Syzygium / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D002244:Carbon; D002265:Carboxylic Ester Hydrolases; C022711:tannase; D009584:Nitrogen