PEG-modified gold nanorods with a stealth character for in vivo applications.
Gold nanorods prepared in hexadecyltrimethylammonium bromide (CTAB) solution are expected to provide novel materials for photothermal therapy and photo-controlled drug delivery systems. Since gold nanorods stabilized with CTAB show strong cytotoxicity, we developed a technique to modify these with polyethyleneglycol (PEG) for medical applications. PEG-modification was achieved by adding mPEG-SH in the CTAB solution, then, excess CTAB was removed by dialysis. PEG-modified gold nanoparticles showed a nearly neutral surface, and had little cytotoxicity in vitro. Following intravenous injection into mice, 54% of injected PEG-modified gold nanoparticles were found in blood at 0.5 h after intravenous injection, whereas most of gold was detected in the liver in the case of original gold nanorods stabilized with CTAB.
Niidome
Takuro
T
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. niidome-tcm@mbox.nc.kyushu-u.ac.jp <niidome-tcm@mbox.nc.kyushu-u.ac.jp>
Yamagata
Masato
M
Okamoto
Yuri
Y
Akiyama
Yasuyuki
Y
Takahashi
Hironobu
H
Kawano
Takahito
T
Katayama
Yoshiki
Y
Niidome
Yasuro
Y
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000633:toxicity; D006046:Gold / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D049329:Nanostructures; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D014018:Tissue Distribution
D002593:Cetrimonium Compounds; D011092:Polyethylene Glycols; D006046:Gold; D000077286:Cetrimonium