title: Purification, crystallization and preliminary X-ray crystallographic analysis of rice bifunctional alpha-amylase/subtilisin inhibitor from Oryza sativa.
abstract: Rice bifunctional alpha-amylase/subtilisin inhibitor (RASI) can inhibit both alpha-amylase from larvae of the red flour beetle (Tribolium castaneum) and subtilisin from Bacillus subtilis. The synthesis of RASI is up-regulated during the late milky stage in developing seeds. The 8.9 kDa molecular-weight RASI from rice has been crystallized using the hanging-drop vapour-diffusion method. According to 1.81 angstroms resolution X-ray diffraction data from rice RASI crystals, the crystal belongs to space group P2(1)2(1)2, with unit-cell parameters a = 79.99, b = 62.95, c = 66.70 angstroms. Preliminary analysis indicates two RASI molecules in an asymmetric unit with a solvent content of 44%.
authors.affiliation: Life Science Group, Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Yi Hung
authors.initials: YH
authors.lastname: Lin
authors.forename: Wen Yan
authors.initials: WY
authors.lastname: Peng
authors.forename: Yen Chieh
authors.initials: YC
authors.lastname: Huang
authors.forename: Hong Hsiang
authors.initials: HH
authors.lastname: Guan
authors.forename: Ying Cheng
authors.initials: YC
authors.lastname: Hsieh
authors.forename: Ming Yih
authors.initials: MY
authors.lastname: Liu
authors.forename: Tschining
authors.initials: T
authors.lastname: Chang
authors.forename: Chun Jung
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D012275:Oryza / Q000201:enzymology*; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013381:Subtilisins / Q000037:antagonists & inhibitors*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D010940:Plant Proteins; D000516:alpha-Amylases; D013381:Subtilisins