Fast adaptation in mouse olfactory sensory neurons does not require the activity of phosphodiesterase.
Vertebrate olfactory sensory neurons rapidly adapt to repetitive odorant stimuli. Previous studies have shown that the principal molecular mechanisms for odorant adaptation take place after the odorant-induced production of cAMP, and that one important mechanism is the negative feedback modulation by Ca2+-calmodulin (Ca2+-CaM) of the cyclic nucleotide-gated (CNG) channel. However, the physiological role of the Ca2+-dependent activity of phosphodiesterase (PDE) in adaptation has not been investigated yet. We used the whole-cell voltage-clamp technique to record currents in mouse olfactory sensory neurons elicited by photorelease of 8-Br-cAMP, an analogue of cAMP commonly used as a hydrolysis-resistant compound and known to be a potent agonist of the olfactory CNG channel. We measured currents in response to repetitive photoreleases of cAMP or of 8-Br-cAMP and we observed similar adaptation in response to the second stimulus. Control experiments were conducted in the presence of the PDE inhibitor IBMX, confirming that an increase in PDE activity was not involved in the response decrease. Since the total current activated by 8-Br-cAMP, as well as that physiologically induced by odorants, is composed not only of current carried by Na+ and Ca2+ through CNG channels, but also by a Ca2+-activated Cl- current, we performed control experiments in which the reversal potential of Cl- was set, by ion substitution, at the same value of the holding potential, -50 mV. Adaptation was measured also in these conditions of diminished Ca2+-activated Cl- current. Furthermore, by producing repetitive increases of ciliary's Ca2+ with flash photolysis of caged Ca2+, we showed that Ca2+-activated Cl- channels do not adapt and that there is no Cl- depletion in the cilia. All together, these results indicate that the activity of ciliary PDE is not required for fast adaptation to repetitive stimuli in mouse olfactory sensory neurons.
Boccaccio
Anna
A
International School for Advanced Studies, S.I.S.S.A., Sector of Neurobiology, 34014 Trieste, Italy. aboccac@sissa.it
Lagostena
Laura
L
Hagen
Volker
V
Menini
Anna
A
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000032:analysis / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D018118:Chloride Channels / Q000502:physiology; D002712:Chlorides / Q000378:metabolism; D002923:Cilia / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D000242:Cyclic AMP / Q000032:analysis / Q000494:pharmacology; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D054815:Cyclic Nucleotide-Gated Cation Channels; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005942:Gluconates / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008027:Light; D051379:Mice; D008807:Mice, Inbred BALB C; D009473:Neuronal Plasticity / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D010777:Photochemistry
D000067956:Adenylyl Cyclase Inhibitors; D018118:Chloride Channels; D002712:Chlorides; D054815:Cyclic Nucleotide-Gated Cation Channels; D004791:Enzyme Inhibitors; D005942:Gluconates; D007473:Ion Channels; D010726:Phosphodiesterase Inhibitors; C017759:9-(tetrahydro-2-furyl)-adenine; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D000225:Adenine; C030691:gluconic acid; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine