title: Reverse optical probing (ROPING) of neocortical circuits.
abstract: We describe an optical technique to detect circuits of synaptically connected neurons. By combining calcium imaging of the spontaneous activity of neuronal populations with intracellular recordings from a given neuron, we perform a type of reverse correlation analysis to detect neurons that generate action potentials time-locked to the synaptic currents of the recorded cell. This technique can quickly reveal monosynaptically connected neurons.
authors.affiliation: HHMI, Department Biological Sciences, Columbia University, New York, USA. gaaron@wesleyan.edu
authors.forename: Gloster
authors.initials: G
authors.lastname: Aaron
authors.forename: Rafael
authors.initials: R
authors.lastname: Yuste
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging / Q000295:instrumentation*; D004594:Electrophysiology; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D019579:Neocortex / Q000033:anatomy & histology* / Q000166:cytology; D009434:Neural Pathways / Q000033:anatomy & histology* / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000502:physiology