Development of capillary electrophoresis  methods for quantitative determination of  taurine in vehicle system and biological  media.
CE methods have been developed for the determination of taurine in pharmaceutical formulation (microemulsion) and in biological media such as sweat. The CE system with end-column pulsed amperometric detection has been found to be an interesting method in comparison with UV and fluorescence detection for its simplicity and rapidity. A gold-disk electrode of 100 mm diameter was used as the working electrode. The effects of a field decoupler at the end of the capillary, separation voltage, injection and pressure times were investigated. A detection limit of 4 x 10(-5) mol/L was reached using integrated pulsed amperometric detection, a method successfully applied to taurine analysis of the biological samples such as sweat. For taurine analysis of oil-in-water microemulsion, fluorescence detector was the favored method, the detection limit of which was 4 x 10(-11) mol/L.
da Silva
Dayse L P
DL
Institute of Pharmaceutical Technology and Biopharmacy, Martin Luther University, Halle-Wittenberg, Germany.
Rüttinger
Hans H
HH
Mrestani
Yahia
Y
Baum
Walter F
WF
Neubert
Reinhard H H
RH
D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D014677:Pharmaceutical Vehicles; D012680:Sensitivity and Specificity; D013542:Sweat / Q000737:chemistry*; D013654:Taurine / Q000032:analysis*
D014677:Pharmaceutical Vehicles; D013654:Taurine; D006046:Gold