title: Determinations of the inclusion complex between gossypol and beta-cyclodextrin.
abstract: Features of the inclusion complex between gossypol (Gos) and beta-cyclodextrin (CD), such as its aqueous solubility, association constant, characteristics in the solid state and crystalline morphology, as well as the stoichiometry of this complex have been determined. The phase-solubility diagram drawn using UV detections belongs to an AN-type. Fluorescence detection and calculation with the modified Benesi-Hidebrond equation provide an 1:2 stoichiometry for the complex. Its apparent stability constant has been determined to be 3,203 M(-1) by fluorescence technique and confirmed by the calculation from UV spectroscopy. X-ray powder diffractions (XRD) and Scanning Electron Microscopy (SEM) observations showed a clear difference in the crystal morphology of the complex from those of both Gos and beta-CD.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Ying-lin
authors.initials: YL
authors.lastname: Shen
authors.forename: Wei
authors.initials: W
authors.lastname: Ying
authors.forename: Sheng-hua
authors.initials: SH
authors.lastname: Yang
authors.forename: Long-min
authors.initials: LM
authors.lastname: Wu
mesh_terms: D003505:Cyclodextrins / Q000737:chemistry*; D006072:Gossypol / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D003505:Cyclodextrins; D006072:Gossypol