title: [The study on the extraction and the antivirus activity of amylose extracted from Grateloupia filicina].
abstract: OBJECTIVE
  To determine the activity of anti-Herpes simplex II virus (HSV-2) of amylose extracted from Grateloupia filicina.
  
  
  METHODS
  Grateloupia filicina amylose was extracted by five kinds of abstraction methods and its suppression on Herpes simplex II virus was observed on cell level in three aspects: the drug activity of protecting cell, the drug influence of HSV-2 proliferation and the drug synthesis action of HSV-2.
  
  
  RESULTS
  Grateloupia filicina had prominence anti-Herpes simplex II virus activity, IC50 of amylose extracted by water distilling and ethanol sedimentation was 5.80 microg/ml.
  
  
  CONCLUSIONS
  It suggest that the antivirus activity happen in the stage of HSV-2 binding, adsorption and ingression with Vero cell.
authors.affiliation: Jinan University, Guangzhou 510632, China.
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Zhu
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Kang
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Men
authors.forename: Ying-Zhou
authors.initials: YZ
authors.lastname: Chen
mesh_terms: D000688:Amylose / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D000431:Ethanol; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry; D000461:Rhodophyta / Q000737:chemistry*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects; D014867:Water
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D014867:Water; D000431:Ethanol; D000688:Amylose