Genotyping of Histomonas meleagridis isolates based on Internal Transcribed Spacer-1 sequences.
C-profiling is a novel genotyping method for protozoan pathogens, based on polymerase chain reaction and sequencing of AT-rich Internal Transcribed Spacer-1 sequences. It was applied to various Histomonas meleagridis isolates originating from outbreaks of histomoniasis in six Dutch turkey and chicken flocks. Three different H. meleagridis genotypes were identified. Type I and type II were associated with clinical disease. In two flocks, both having recovered from an outbreak of histomoniasis, a type III strain was found that was also morphologically slightly different from the type I and type II isolates. C-profiling is a promising technique to differentiate between H. meleagridis subtypes, making it useful for epidemiological studies.
van der Heijden
Harold M J F
HM
h.vd.hejden@gdvdieren.nl
Landman
Wil J M
WJ
Greve
Sophie
S
Peek
Ron
R
D000818:Animals; D001717:Birds / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D056890:Eukaryota / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods
D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer