[Conservative nucleotide sites in promoters of tobamoviral subgenomic RNA].
A computer search for position-linked identical nucleotide sites in sequences of promoter regions of subgenomic RNA and coat protein of fifteen tobamoviruses was performed. It was discovered that promoters of three TMV strains tested as well as of tobacco mild green mosaic virus and turnip vein-clearing virus contain the nine-nucleotide sites gattcgttt or ggttcgttt. These sites are localized at 14-77 nucleotide upstream from the translation start codons of coat or movement protein genes and are flanged with length-variable gc- (upstream) and at-containing sequences (downstream). The middle part of the nine-nucleotide sites (tcgtt) is present in 22 of 30 promoter regions tested. The role of the conservative sites found as functional elements of tobamoviral subgenomic RNA promoters is discussed.
Kyrychenko
A M
AM
Shcherbatenko
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D001483:Base Sequence; D001665:Binding Sites; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014027:Tobacco Mosaic Virus / Q000235:genetics; D017898:Tobamovirus / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
D012367:RNA, Viral; D014764:Viral Proteins