Mass spectral analysis of the apolipoproteins on mouse high density lipoproteins. Detection of post-translational modifications.
Using mass spectrometry, we have recently reported on molecular masses of the apolipoproteins associated with porcine and equine HDL. In addition to obtaining accurate masses for the various apolipoproteins, we also were able to detect mass variations due to post-translational modifications. In the present study, we have used these same approaches to characterize the apolipoproteins in two inbred mouse strains, C57BL/6 and BALB/c. Comparing our molecular mass data with calculated values for molecular weight, we were able to identify the correct sequences for several of the major apolipoproteins. Analyses were carried out on the apolipoproteins of ultracentrifugally isolated HDL. Prior to analyses by electrospray ionization mass spectrometry (ESI-MS), the apolipoproteins were separated either by size exclusion or reverse phase chromatography. The molecular masses of apoA-I, proapoA-I, apoA-II, proapoA-II, apoC-I and apoC-III were obtained. Comparing the values obtained for the two strains, differences in the molecular masses of apoA-I, apoA-II and apoC-III were observed. In this study, post-translationally modified apolipoproteins, involving loss of amino acids from both the N- and C-termini, oxidation of methionine residues and possible acylation, were noted following reverse-phase separation. Further analyses by tandem mass spectrometry (MSMS) done on the tryptic digests of apolipoproteins separated by reverse phase chromatography enabled us to confirm sequence differences between the two strains, to verify selected apoA-I sequences that had been entered into the GenBank and to identify which methionines in apoA-I, apoC-III and apoE had been converted to methionine sulfoxides.
Puppione
Donald L
DL
The Molecular Biology Institute and The Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA. puppione@chem.ucla.edu
Yam
Lang M
LM
Bassilian
Sara
S
Souda
Puneet
P
Castellani
Lawrence W
LW
Schumaker
Verne N
VN
Whitelegge
Julian P
JP
D000595:Amino Acid Sequence; D000818:Animals; D001053:Apolipoproteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008075:Lipoproteins, HDL / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D014357:Trypsin
D001053:Apolipoproteins; D008075:Lipoproteins, HDL; D010446:Peptide Fragments; D014357:Trypsin