[Characterization and phylogenetic analysis of the cytochrome oxidase subunit I gene of mitochondrial genome from Takifugu fasciatus].
The Takifugu fasciatus mitochondrial cytochrome oxidase I gene (COI) and its associated tRNA genes were sequenced by PCR. The open reading frame of COI gene contains 1,546 bp nucleotides, encoding a putative protein of 515 amino acid residues. The pattern of codon usage of the COI gene is less biased toward A+T. The COI gene of T. fasciatus shows a high degree of homology with that from the other 14 fish species recorded in the GenBank and has 97.6% homology with Takifugu rubripes, 76.5% with Masturus lanceolatus and 75.4% with Mola mola. The phylogenetic trees show that the relationships based on the homology is consistent with the morphological and taxonomic results. Predicted secondary structures of the tRNA genes suggest that they have the classical cloverleaf structures.
Shao
Ai-Hua
AH
Life Sciences School, Soochow University, Suzhou 215123, China. ahs6892@yahoo.com.cn
Zhu
Jiang
J
Chen
Kui
K
Shi
Quan-Liang
QL
Yao
Wei-Wen
WW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics*; D005399:Fishes / Q000145:classification / Q000235:genetics; D054629:Genome, Mitochondrial; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D023741:Takifugu / Q000145:classification / Q000235:genetics*
D029941:Fish Proteins; D024101:Mitochondrial Proteins; D003576:Electron Transport Complex IV