Polymorphism of the major surface epitope of the CopB outer membrane protein of Moraxella catarrhalis.
The CopB outer membrane protein has been considered a vaccine candidate for the prevention of infections due to Moraxella catarrhalis. Monoclonal antibody 10F3 recognizes whole cells of about 70% of clinical isolates, suggesting that this epitope is reasonably conserved. To determine whether CopB has other surface epitopes, we analyzed M. catarrhalis isolates using polyclonal sera against recombinant CopB proteins from a 10F3 positive isolate and a 10F3 negative isolate, and polyclonal sera against synthetic peptides that contained the sequence corresponding to the 10F3 epitope region of three different isolates. Extensive cross-reactivity was observed with the anti-CopB sera towards purified recombinant CopB proteins in Western blot and antigen ELISA, implying that antigenic regions common to both proteins were present. However, anti-CopB sera resembled anti-CopB peptide sera in exhibiting similar binding specificity to whole cells, segregating M. catarrhalis isolates into four CopB groups. We subsequently cloned and sequenced the copB genes from representative isolates. The deduced CopB amino acid sequences and the degree of sequence identity also demonstrated the existence of the same four CopB groups. Each of the four groups had a unique sequence in the 10F3 epitope region and a fifth group had the epitope deleted. The polymorphism of the major surface epitope prompts further consideration regarding the utility of CopB as a vaccine component as well as the design of an efficacious CopB-based vaccine to achieve broad protection against Moraxella infection.
Liu
Dai-Fang
DF
Vaccines Research, Wyeth, Pearl River, NY 10965, USA. liudf@wyeth.com
Xie
Xiaoling
X
Mastri
Maria G
MG
Fortuna-Nevin
Maria
M
Colocillo
Christopher
C
Fletcher
Leah
L
Dilts
Deborah A
DA
McMichael
John C
JC
Baker
Steven M
SM
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D001936:Moraxella catarrhalis / Q000276:immunology* / Q000302:isolation & purification; D011110:Polymorphism, Genetic; D011994:Recombinant Proteins / Q000276:immunology
D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; C093197:CopB protein, Moraxella; D000939:Epitopes; D007106:Immune Sera; D011994:Recombinant Proteins