Cloning of a CD59-like gene in rainbow trout. Expression and phylogenetic analysis of two isoforms.
CD59, the major inhibitor of the complement membrane attack complex, is an 18-20 kDa glycoprotein, linked to the membrane via a glycosylphosphatidylinositol (GPI)-anchor. It restricts binding of C9 to the C5b-8 complex, preventing the formation of the complement membrane attack complex C5b-9. In this study we report the cloning of a second CD59-like gene in the rainbow trout, Oncorhynchus mykiss (referred to as CD59-2 and the previously deposited trout CD59-like gene as CD59-1). Trout CD59-2 is 56% identical to CD59-1 at the amino acid level. Both of trout CD59s show the highest identity score (54%) with putative CD59-like molecules from other teleost, and the overall identity with their mammalian orthologs is less than 30%. Trout CD59s are expressed in brain, heart, intestine, kidney, liver and spleen. Particularly, CD59-2 is abundant in trout brain, while CD59-1 seems to be absent in the trout spleen. Moreover, both of trout CD59 genes seems to be present as a single copy in trout genome.
Papanastasiou
Anastasios D
AD
Department of Biology, School of Medicine, University of Patras, Rion, 26500 Patras, Greece.
Georgaka
Eleftheria
E
Zarkadis
Ioannis K
IK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018957:CD59 Antigens / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D010802:Phylogeny; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D016415:Sequence Alignment
D018957:CD59 Antigens; D018076:DNA, Complementary; D020033:Protein Isoforms