title: In vitro activities of ceftobiprole, tigecycline, daptomycin, and 19 other antimicrobials against methicillin-resistant Staphylococcus aureus strains from a national survey of Belgian hospitals.
abstract: The in vitro activities of 22 antimicrobial agents, including ceftobiprole, daptomycin, and tigecycline, against 511 methicillin-resistant Staphylococcus aureus (MRSA) isolates from 112 Belgian hospitals were studied by using the CLSI agar dilution method. Isolates were characterized by pulsed-field gel electrophoresis (PFGE) analysis and by PCR detection of determinants of resistance to aminoglycosides, macrolides-lincosamides-streptogramins, and tetracyclines. A representative set of isolates with different PFGE genotypes was further characterized by multilocus sequence typing, determination of staphylococcal cassette chromosome mec (SCCmec) type, and multiplex PCR for toxic shock syndrome type 1 (TSST-1) and Panton-Valentine leukocidin genes. MRSA isolates belonged to nine epidemic MRSA clones, of which sequence type 45 (ST45)-SCCmec IV and ST8-SCCmec IV were predominant, accounting for 49 and 20% of isolates, respectively. The distribution of antimicrobial resistance and TSST-1 genes was strongly linked to clonal types. Ceftobiprole, daptomycin, and tigecycline showed high activity against all isolates of these sporadic and epidemic MRSA clones, as indicated by MIC(90)s of 2 mg/liter, 0.5 mg/liter, and 0.25 mg/liter, respectively. The MIC distribution of daptomycin and tigecycline was not different in isolates with decreased susceptibility to glycopeptides or tetracyclines, respectively. Ceftobiprole MICs were not correlated with oxacillin and cefoxitin MICs. These data indicate excellent activity of the newly developed agents ceftobiprole, daptomycin, and tigecycline against MRSA isolates recently recovered from hospitalized patients in Belgium, supporting their therapeutic potential for nosocomial MRSA infections.
authors.affiliation: Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, 1070 Brussels, Belgium. odenis@ulb.ac.be
authors.forename: Olivier
authors.initials: O
authors.lastname: Denis
authors.forename: Ariane
authors.initials: A
authors.lastname: Deplano
authors.forename: Claire
authors.initials: C
authors.lastname: Nonhoff
authors.forename: Marie
authors.initials: M
authors.lastname: Hallin
authors.forename: Raf
authors.initials: R
authors.lastname: De Ryck
authors.forename: Raymond
authors.initials: R
authors.lastname: Vanhoof
authors.forename: Ricardo
authors.initials: R
authors.lastname: De Mendonça
authors.forename: Marc J
authors.initials: MJ
authors.lastname: Struelens
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001530:Belgium / Q000453:epidemiology; D002511:Cephalosporins / Q000494:pharmacology*; D017576:Daptomycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005098:Exotoxins / Q000235:genetics; D005798:Genes, Bacterial; D006761:Hospitals; D006801:Humans; D066298:In Vitro Techniques; D016106:Methicillin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000379:methods; D008911:Minocycline / Q000031:analogs & derivatives* / Q000494:pharmacology; D017720:Molecular Epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000078304:Tigecycline
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D005098:Exotoxins; C443755:ceftobiprole; D000078304:Tigecycline; D008911:Minocycline; D017576:Daptomycin