title: Differential effect of IFN-tau on proliferation and distribution of lymphocyte subsets in one-way mixed lymphocyte reaction in cows and heifers.
abstract: IFN-tau is a signaling protein secreted by the bovine conceptus during the peri-implantation period and responsible for pregnancy recognition. Its main role is the prevention of pulsatile release of luteolytic PGF2alpha, but it also exerts immunomodulatory activities characteristic for other type I interferons. The aim of the study was to examine the effect of IFN-tau on the proliferation and distribution of peripheral blood lymphocyte subsets during one-way mixed lymphocyte reaction (MLR) in cows and heifers. IFN-tau inhibited the proliferative response of lymphocytes in MLR both in cows and heifers in a dose-dependent manner, but cow lymphocytes were less susceptible than those ones from heifers. It was also showed that IFN-tau differentially changed lymphocyte subsets distribution in MLR in cows and heifers. In cows, the relative percentage of CD8(+) cells after MRL in the presence of IFN-tau was significantly lower than in heifers. Differential effect of rIFN-tau on proliferation and lymphocyte subsets distribution in a one-way MRL in cows and heifers indicated that the age of the mother is an important factor in immunomodulatory effect towards developing bovine embryo.
authors.affiliation: Faculty of Veterinary Medicine, Agriculture University of Wrocław, Norwida Str. 31, 50-375 Wroclaw, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Gierek
authors.forename: D
authors.initials: D
authors.lastname: Baczyńska
authors.forename: M
authors.initials: M
authors.lastname: Ugorski
authors.forename: F
authors.initials: F
authors.lastname: Bazer
authors.forename: M
authors.initials: M
authors.lastname: Kurpisz
authors.forename: T
authors.initials: T
authors.lastname: Bednarski
authors.forename: M
authors.initials: M
authors.lastname: Gorczykowski
authors.forename: A
authors.initials: A
authors.lastname: Chełmońska-Soyta
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation; D005260:Female; D007370:Interferon Type I / Q000502:physiology*; D007959:Lymphocyte Culture Test, Mixed; D016131:Lymphocyte Subsets / Q000166:cytology*; D008297:Male; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000502:physiology*
chemical_list: D007370:Interferon Type I; D011257:Pregnancy Proteins; C020947:interferon tau