title: Analysis of sperm aneuploidy by PRINS.
abstract: Based on the direct in situ mixing of the colors of different fluorochromes (fluorescein isothiocyanate, tetramethylrhodamine isothiocyanate, Cascade Blue) incorporated in sequential primed in situ labeling (PRINS) reactions, a new multicolor PRINS procedure is described, allowing the rapid and distinct in situ labeling of three or four human chromosomes. Each PRINS reaction consists of a unique 5-min step for annealing and elongation. In combination with the 0.5 M NaOH pretreatment for simultaneous in situ denaturation and decondensation of sperm nuclei, this technique has been adapted to human sperm nuclei for the direct assessment of aneuploidy.
authors.affiliation: Institute of Human Genetics, CNRS UPR 1142, Montpellier, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Pellestor
authors.forename: Brigitte
authors.initials: B
authors.lastname: Andréo
authors.forename: Jacques
authors.initials: J
authors.lastname: Puechberty
authors.forename: Geneviève
authors.initials: G
authors.lastname: Lefort
authors.forename: Pierre
authors.initials: P
authors.lastname: Sarda
mesh_terms: D000782:Aneuploidy; D002877:Chromosomes, Human / Q000235:genetics; D006801:Humans; D008297:Male; D020401:Primed In Situ Labeling / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*