title: Catalytic domain of AfsKav modulates both secondary metabolism and morphologic differentiation in Streptomyces avermitilis ATCC 31272.
abstract: Genetic characterization of afsK-av (SAV3816) in Streptomyces avermitilis ATCC 31272 was performed to evaluate the role(s) of this eukaryotic-type serine-threonine protein kinase (STPK) in the regulation of morphologic differentiation and secondary metabolism. The afsK-av::neo mutant (SJW4001) was defective in sporulation, melanogenesis, and avermectin production. These phenotypic defects were complemented by introduction of either the intact afsK-av or the 900-nt catalytic domain region. The catalytic domain restored sporulation and melanogenesis to SJW4001 whereas it partially recovered avermectin production. This study reveals that AfsKav is a pleiotropic regulator and demonstrates in vivo that the C-region of AfsKav is not essential for its regulatory role in S. avermitilis differentiations.
authors.affiliation: Department of Biologic Science, Institute of Bioscience and Biotechnology, Myongji University, Yongin 449-728, Korea.
authors.forename: A
authors.initials: A
authors.lastname: Rajkarnikar
authors.forename: H-J
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Y-W
authors.initials: YW
authors.lastname: Ryu
authors.forename: J-W
authors.initials: JW
authors.lastname: Suh
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain / Q000235:genetics*; D005816:Genetic Complementation Test / Q000379:methods; D007559:Ivermectin / Q000031:analogs & derivatives / Q000378:metabolism; D008543:Melanins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D013171:Spores, Bacterial / Q000235:genetics / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D008543:Melanins; D007559:Ivermectin; C019264:avermectin; D017346:Protein Serine-Threonine Kinases