BDNF regulation in the rat dorsal vagal complex during stress-induced anorexia.
The dorsal vagal complex (DVC) is the satiety reflex-integrating center of adult mammals. Immobilization stress (IS) is known to elicit anorexia and to up-regulate BDNF expression in adult rat forebrain; intra-DVC delivery of BDNF was shown to elicit anorexia. Therefore, we addressed here whether IS would increase BDNF signaling in rat DVC by using PCR and western-blot on microdissected tissue extracts. Significant variations of BDNF expression in DVC after IS include exon V mRNA increase at 3 h, decreases of both protein and exon III mRNA at 24 h, and exon I mRNA decrease at 72 h. At the receptor level, IS elicited a highly significant induction of both full-length and truncated-1 TrkB mRNAs at 24 h after IS. In vivo recruitment of BDNF signaling in DVC during stress thus differs from hypothalamus, the relevance of which to anorexia is discussed.
Charrier
Céline
C
Lab Physiologie Neurovégétative, UMR 6153 CNRS-1147 INRA, Université Paul Cézanne, Avenue Escadrille Normandie-Niemen, 13397 Marseille cedex 20, France.
Chigr
Fatiha
F
Tardivel
Catherine
C
Mahaut
Stéphanie
S
Jean
André
A
Najimi
Mohamed
M
Moyse
Emmanuel
E
D000704:Analysis of Variance; D000818:Animals; D000855:Anorexia / Q000209:etiology / Q000378:metabolism*; D001835:Body Weight / Q000502:physiology; D001933:Brain Stem / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020813:Receptor, trkB / Q000235:genetics / Q000378:metabolism; D012149:Restraint, Physical / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013312:Stress, Physiological / Q000150:complications; D013997:Time Factors
D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D020813:Receptor, trkB