Functional analysis of an endo-1,6-beta-D-glucanase gene (neg-1) from Neurospora crassa.
The 1,6-beta-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.
Oyama
Seiji
S
Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
Inoue
Hirokazu
H
Yamagata
Youhei
Y
Nakajima
Tasuku
T
Abe
Keietu
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D003062:Codon; D003224:Congo Red / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D017354:Point Mutation; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D002593:Cetrimonium Compounds; D003062:Codon; D012967:Sodium Dodecyl Sulfate; D003224:Congo Red; D006026:Glycoside Hydrolases; C020105:endo-1,6-beta-glucanase; D000077286:Cetrimonium