[The correlated research of acute graft versus host disease after allogeneic stem cell transplantation].
OBJECTIVE
To analyze the relationship between the expression of FasL, Perforin and Granzyme B and the development of acute graft versus host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT).


METHODS
The peripheral blood mRNA expression of granzyme B, perforin, fasL from 17 patients after allo-HSCT was detected by competitive quantitative RT-PCR and the relationship between FasL, Granzyme B and Perforin expressions and clinical symptom of aGVHD was analyzed.


RESULTS
The expression level of Granzyme B, Perforin and FasL was 4.6 +/- 0.2, 4.5 +/- 0.1, 1.4 +/- 0.1 before aGVHD occurrence respectively, and was 98.7 +/- 2.5, 91.8 +/- 3.4, 61.5 +/- 2.2, after the occurrence in 14 patients (P < or = 0.05). Over expressions of Granzyme B, Perforin, and FasL during acute GVHD were detected in 13 of 14, 12 of 14, and 12 of 14 patients respectively. The upregulated expressions occurred prior to clinical symptom of aGVHD.


CONCLUSIONS
The expressions of Granzyme B, Perforin, and FasL were significantly high in patients with acute aGVHD. Monitoring of the expressions, might predict the occurrence of clinical aGVHD and it severity and prognosis.
Yang
Fan
F
The Aoademy of military Medical and Science, Beijing 100039, China.
Chen
Hu
H
Liu
Guang-xian
GX
Lu
Ming
M
Feng
Kai
K
Zhang
Ying-ge
YG
Zhang
Jing
J
D000208:Acute Disease; D000328:Adult; D053222:Fas Ligand Protein / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006086:Graft vs Host Disease / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D053804:Granzymes / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D006801:Humans; D008297:Male; D054353:Perforin / Q000235:genetics; D011183:Postoperative Complications / Q000097:blood / Q000175:diagnosis / Q000209:etiology; D011379:Prognosis; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014184:Transplantation, Homologous
D053222:Fas Ligand Protein; D012333:RNA, Messenger; D054353:Perforin; D053804:Granzymes