title: Cardiac sodium channel Nav1.5 is regulated by a multiprotein complex composed of syntrophins and dystrophin.
abstract: The cardiac sodium channel Na(v)1.5 plays a key role in cardiac excitability and conduction. The purpose of this study was to elucidate the role of the PDZ domain-binding motif formed by the last three residues (Ser-Ile-Val) of the Na(v)1.5 C-terminus. Pull-down experiments were performed using Na(v)1.5 C-terminus fusion proteins and human or mouse heart protein extracts, combined with mass spectrometry analysis. These experiments revealed that the C-terminus associates with dystrophin, and that this interaction was mediated by alpha- and beta-syntrophin proteins. Truncation of the PDZ domain-binding motif abolished the interaction. We used dystrophin-deficient mdx(5cv) mice to study the role of this protein complex in Na(v)1.5 function. Western blot experiments revealed a 50% decrease in the Na(v)1.5 protein levels in mdx(5cv) hearts, whereas Na(v)1.5 mRNA levels were unchanged. Patch-clamp experiments showed a 29% decrease of sodium current in isolated mdx(5cv) cardiomyocytes. Finally, ECG measurements of the mdx(5cv) mice exhibited a 19% reduction in the P wave amplitude, and an 18% increase of the QRS complex duration, compared with controls. These results indicate that the dystrophin protein complex is required for the proper expression and function of Na(v)1.5. In the absence of dystrophin, decreased sodium current may explain the alterations in cardiac conduction observed in patients with dystrophinopathies.
authors.affiliation: Department of Pharmacology and Toxicology, University of Lausanne, Switzerland.
authors.forename: Bruno
authors.initials: B
authors.lastname: Gavillet
authors.forename: Jean-Sébastien
authors.initials: JS
authors.lastname: Rougier
authors.forename: Andrea A
authors.initials: AA
authors.lastname: Domenighetti
authors.forename: Romina
authors.initials: R
authors.lastname: Behar
authors.forename: Christophe
authors.initials: C
authors.lastname: Boixel
authors.forename: Patrick
authors.initials: P
authors.lastname: Ruchat
authors.forename: Hans-Anton
authors.initials: HA
authors.lastname: Lehr
authors.forename: Thierry
authors.initials: T
authors.lastname: Pedrazzini
authors.forename: Hugues
authors.initials: H
authors.lastname: Abriel
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016189:Dystrophin / Q000502:physiology; D049029:Dystrophin-Associated Proteins / Q000502:physiology; D004562:Electrocardiography; D006321:Heart / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D032342:Muscle Cells / Q000166:cytology / Q000502:physiology*; D009124:Muscle Proteins / Q000235:genetics / Q000502:physiology*; D062554:NAV1.5 Voltage-Gated Sodium Channel; D018408:Patch-Clamp Techniques; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015222:Sodium Channels / Q000235:genetics / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D016189:Dystrophin; D049029:Dystrophin-Associated Proteins; D009124:Muscle Proteins; D062554:NAV1.5 Voltage-Gated Sodium Channel; D011993:Recombinant Fusion Proteins; C568320:SCN5A protein, human; C568321:Scn5a protein, mouse; D015222:Sodium Channels; C091824:syntrophin