title: Electrochemical impedance sensing of DNA hybridization on conducting polymer film-modified diamond.
abstract: The impedimetric sensing of DNA hybridization on polyaniline/polyacrylate (PANI/PAA)-modified boron-doped diamond (BDD) electrode has been investigated. An ultrathin film of PANI-PAA copolymer was electropolymerized onto the diamond surfaces to provide carboxylic groups for tethering to DNA sensing probes. The electrochemical impedance and the intrinsic electroactivity of the polymer-diamond interface were analyzed after the hybridization reaction with target and non-target DNA. The impedance measurement shows changes in the impedance modulus as well as electron-transfer resistance at the stage of probe DNA immobilization (single-strand), as well as after hybridization with target DNA (double-strand). DNA hybridization increases the capacitance of the polymer-DNA layer and reduces the overall impedance of the DNA-polymer-diamond stack significantly. The polymer-modified BDD electrode shows no detectable nonspecific adsorption, with good selectivity between the complementary DNA targets and the one-base mismatch targets. The detection limit was measured to be 2 x 10(-8) M at 1000 Hz. Denaturing test on the hybridized probe and subsequent reuse of the probe indicates chemical robustness of the sensor. Our results suggest that electropolymerization followed by the immobilization of biomolecules is a simple and effective way of creating a functional biomolecular scaffold on the diamond surface. In addition, label-free electrochemical impedance method can provide direct and noninvasive sensing of DNA hybridization on BDD.
authors.affiliation: Institute of Materials Research and Engineering, 3 Research Link, Singapore 117602.
authors.forename: Huiru
authors.initials: H
authors.lastname: Gu
authors.forename: Xiao di
authors.initials: Xd
authors.lastname: Su
authors.forename: Kian Ping
authors.initials: KP
authors.lastname: Loh
mesh_terms: D001895:Boron / Q000737:chemistry; D002627:Chemistry, Physical / Q000379:methods; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D018130:Diamond; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004583:Electrons; D008855:Microscopy, Electron, Scanning; D009693:Nucleic Acid Hybridization; D010710:Phosphates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; D010710:Phosphates; D011108:Polymers; D018130:Diamond; D004247:DNA; D001895:Boron