Crystallization and preliminary X-ray diffraction analysis of BipD, a virulence factor from Burkholderia pseudomallei.
Burkholderia pseudomallei, the causative agent of melioidosis, possesses a protein-secretion apparatus that is similar to those found in Salmonella and Shigella. A major function of these secretion systems is to secrete virulence-associated proteins into target cells of the host organism. The BipD gene of B. pseudomallei encodes a secreted virulence factor that is similar in sequence and most likely functionally analogous to IpaD from Shigella and SipD from Salmonella. Thus, the BipD protein is likely to be a component of a type III protein-secretion system (TTSS) in B. pseudomallei. Proteins in the same class as BipD, such as IpaD and SipD, are thought to act as extracellular chaperones to help the hydrophobic translocator proteins enter the target cell membrane, where they form a pore and might even link the translocon pore with the secretion needle. There is evidence that the translocator proteins also bind an integrin which stimulates actin-mediated insertion of the bacterium into the host-cell membrane. Native BipD has been crystallized in a monoclinic crystal form that diffracts X-rays to 2.5 angstroms resolution. BipD protein which incorporates selenomethionine (SeMet-BipD) has also been expressed and forms crystals which diffract to a higher resolution of 2.1 angstroms.
Knight
M J
MJ
School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, England.
Ruaux
A
A
Mikolajek
H
H
Erskine
P T
PT
Gill
R
R
Wood
S P
SP
Wood
M
M
Cooper
J B
JB
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D016957:Burkholderia pseudomallei / Q000472:pathogenicity*; D003460:Crystallization; D012645:Selenomethionine / Q000378:metabolism; D012680:Sensitivity and Specificity; D037521:Virulence Factors / Q000737:chemistry* / Q000302:isolation & purification; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D037521:Virulence Factors; D012645:Selenomethionine