title: Determination of organochlorine and pyrethroid pesticides in fruit and vegetables using solid phase extraction clean-up cartridges.
abstract: A method to determine six organochlorine and three pyrethroid pesticides in grape, orange, tomato, carrot and green mustard based on solvent extraction followed by solid phase extraction (SPE) clean-up is described. The pesticides were spiked into the sample prior to analysis, extracted with ethyl acetate, evaporated and reconstituted with a solvent mixture of acetone:n-hexane (3:7). Three different sorbents (Strong Anion Exchanger/Primary Secondary Amine (SAX/PSA), Florisil and C18) were used for the clean-up step. Pesticides were eluted with 5mL of acetone:n-hexane (3:7, v/v) and determined by gas chromatography and electron-capture detection (GC-ECD). SAX/PSA was the sorbent, which provided chromatograms with less interference and the mean recoveries obtained were within 70-120% except for captafol. The captafol recoveries for grape were within acceptable range with C18 clean-up column.
authors.affiliation: National Public Health Laboratory, Ministry of Health, Malaysia Lot 1853, Kpg. Melayu Sungai Buloh, 47000 Sungai Buloh, Selangor, Malaysia.
authors.forename: Zawiyah
authors.initials: Z
authors.lastname: Sharif
authors.forename: Yaakob Bin Che
authors.initials: YB
authors.lastname: Man
authors.forename: Nazimah Sheikh Abdul
authors.initials: NS
authors.lastname: Hamid
authors.forename: Chin Cheow
authors.initials: CC
authors.lastname: Keat
mesh_terms: D005591:Chemical Fractionation / Q000379:methods; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D011722:Pyrethrins