[Construction of eukaryotic expression recombinant plasmid of trichomonas vaginalis ferredoxin gene].
Total DNA was extracted from T. vaginalis with Chelex-100 method and used as templates for PCR. The ferredoxin gene was directionally cloned into plasmid pMD-18T vector and subcloned into eukaryotic expression vector pcDNA3. 1 (+). The transformants were screened and identified by PCR and restriction analysis. The size of amplified ferredoxin gene was 306bp and the DNA sequence of cloned gene was same with that published.
Xie
Hui
H
Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610044, China.
Wang
Ya-jing
YJ
Tie
Chao-nan
CN
Bi
Shi-liang
SL
Liu
Pei-na
PN
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005057:Eukaryotic Cells / Q000378:metabolism*; D005288:Ferredoxins / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics*; D014246:Trichomonas vaginalis / Q000235:genetics*
D005288:Ferredoxins; D015800:Protozoan Proteins