Detecting transcriptionally active regions using genomic tiling arrays.
We have developed a method for interpreting genomic tiling array data, implemented as the program TranscriptionDetector. Probed loci expressed above background are identified by combining replicates in a way that makes minimal assumptions about the data. We performed medium-resolution Anopheles gambiae tiling array experiments and found extensive transcription of both coding and non-coding regions. Our method also showed improved detection of transcriptional units when applied to high-density tiling array data for ten human chromosomes.
Halasz
Gabor
G
Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, New York, NY 10027, USA. gh74@columbia.edu
van Batenburg
Marinus F
MF
Perusse
Joelle
J
Hua
Sujun
S
Lu
Xiang-Jun
XJ
White
Kevin P
KP
Bussemaker
Harmen J
HJ
D000818:Animals; D000852:Anopheles / Q000235:genetics; D002877:Chromosomes, Human; D023281:Genomics; D006801:Humans; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic