title: Intestinal damage in strongyloidiasis: the imbalance between cell death and proliferation.
abstract: Strongyloidiasis is an endemic tropical parasitosis caused by Strongyloides stercoralis that also affects immigrants in nontropical countries. The nematode colonizes the duodenum and upper jejunum, inducing mucosal alterations. Because integrity is essential for a functional barrier, we aimed to study apoptosis and proliferation in the small bowel epithelium infected with S. stercoralis. We evaluated 23 patients and 17 controls. Apoptotic cells were detected by TUNEL and M30 immunolabelling, whereas proliferation was scored by Ki67 immunostaining and mitotic counting. Infection increased apoptotic indices in duodenum and jejunum (P < 0.001). Conversely, it decreased cell proliferation in both segments (P < 0.001). Our results showed that intestinal strongyloidiasis promotes an imbalance between cell death and proliferation. This is the first evidence of disruption of the epithelial kinetics with S. stercoralis infection, though the mechanisms remain unclear. Furthermore, our results support the idea that strongyloidiasis disturbs the mucosal integrity and can compromise the intestinal barrier.
authors.affiliation: Department of Gastroenterology, School of Medicine, Laboratory of Investigation (LIM 07), University of São Paulo, São Paulo, Brazil.
authors.forename: Ana Luiza
authors.initials: AL
authors.lastname: Werneck-Silva
authors.forename: Eliana Parisi
authors.initials: EP
authors.lastname: Alvares
authors.forename: Patrícia
authors.initials: P
authors.lastname: Gama
authors.forename: Aderson Omar Mourão Cintra
authors.initials: AO
authors.lastname: Damião
authors.forename: Luciana Harumi
authors.initials: LH
authors.lastname: Osaki
authors.forename: Daniela
authors.initials: D
authors.lastname: Ogias
authors.forename: Aytan Miranda
authors.initials: AM
authors.lastname: Sipahi
mesh_terms: D000328:Adult; D000818:Animals; D017209:Apoptosis; D016022:Case-Control Studies; D049109:Cell Proliferation; D004386:Duodenum / Q000166:cytology / Q000469:parasitology*; D004848:Epithelium / Q000469:parasitology / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007583:Jejunum / Q000166:cytology / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D017171:Strongyloides stercoralis; D013322:Strongyloidiasis / Q000473:pathology*