[Analysis of comparative immunological profiles of intraocular fluid and serum samples in patients suspected of ocular toxoplasmosis or toxocarosis].
High seroprevalence of Toxoplasma and Toxocara spp. in populations of children and adults in Poland constitutes a significant risk of supradiagnosed parasitic eye infections. We described the clinical characteristics in relation to the analysis of comparative immunological profiles of T. gondii-specific antibodies in aqueous humour and serum samples in patients with reactivated retinochoroiditis, and of Toxocara spp. ones in cases with posterior granuloma, fibrotic and calcified tumor-like masses simulating retinoblastoma, detected by ophthalmoscopy and echography. Intraocular synthesis of specific IgG antibodies was detected in anterior eye chamber fluid in 1/2 and 2/3 of patients respectively, strongly suspected of ocular toxoplasmosis or toxocarosis. The evidence of a local production of specific antibodies in intraocular fluid shown by the Western blot seems to be a valuable immunodiagnostic method for a final confirmation of eye lesions of parasitic origin and crucial in the choice of an appropriate treatment regimen.
Paul
Małgorzata
M
Katedra i Klinika Chorób Tropikalnych i Pasozytniczych AM, Poznań.
Stefaniak
Jerzy
J
Pecold
Krystyna
K
Twardosz-Pawlik
Hanna
H
D000293:Adolescent; D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D001082:Aqueous Humor / Q000276:immunology / Q000469:parasitology*; D015415:Biomarkers / Q000032:analysis; D002648:Child; D002825:Chorioretinitis / Q000175:diagnosis; D003937:Diagnosis, Differential; D015822:Eye Infections, Parasitic / Q000097:blood / Q000175:diagnosis; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007815:Larva Migrans / Q000097:blood* / Q000175:diagnosis*; D008875:Middle Aged; D012175:Retinoblastoma / Q000175:diagnosis; D012698:Serologic Tests; D014126:Toxoplasmosis, Ocular / Q000097:blood* / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D015415:Biomarkers; D007136:Immunoglobulins