title: [Study on endophyte and the growth stages of Ophiopogon japonicus].
abstract: OBJECTIVE
  To study on the relationship between the endophyte and the life cycle of Ophiopogon japonicus in Zhejiang.
  
  
  METHODS
  Sample roots of Ophiopogon japonicus at different growth stages were thoroughly washed and cut into small fragments, then cleared (removing cytoplasmic contents from cells) using hot 10% KOH and stained with acid fuchsin (alternative stain). The hyphae, the arbuscular and the vesicular of endophyte were examined.
  
  
  RESULTS
  The hyphae appeared and grew in the seedling stage, the hyphae grew into arbuscular in the root tuber generating stage and vesicular in the stage of root tuber expanding period.
  
  
  CONCLUSIONS
  The endophytes in Ophiopogon japonicus appeared in forms of hyphae, arbuscular and vesicular at different growth stages to meet the needs of Ophiopogon japonicus developing.
authors.affiliation: Department of Life Science, Zhejiang College of TCM, Hangzhou 310053, China. cdq@hztele.com.cn
authors.forename: Dong-Qing
authors.initials: DQ
authors.lastname: Cheng
authors.forename: Zhi-Ren
authors.initials: ZR
authors.lastname: Yu
authors.forename: Pei-Lei
authors.initials: PL
authors.lastname: Pan
authors.forename: Mei-Ai
authors.initials: MA
authors.lastname: Lin
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Chen
mesh_terms: D005658:Fungi / Q000648:ultrastructure; D031441:Ophiopogon / Q000254:growth & development* / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D010946:Plants, Medicinal / Q000254:growth & development* / Q000382:microbiology*; D027343:Rhizome / Q000254:growth & development / Q000382:microbiology; D012621:Seasons