Rx-Cre, a tool for inactivation of gene expression in the developing retina.
Rx is a homeobox-containing gene that is critical for vertebrate eye development. Its expression domain delineates a field of cells from which the retina and the ventral hypothalamus develop. The 5' upstream regulatory sequences of the medaka fish Rx gene are functionally conserved during evolution to a degree that they direct gene expression into the Rx-expressing field of cells in mice. Using these sequences, we made a Cre line that can be used for inactivation of gene expression in the developing retina.
Swindell
Eric C
EC
Bailey
Travis J
TJ
Loosli
Felix
F
Liu
Chaomei
C
Amaya-Manzanares
Felipe
F
Mahon
Kathleen A
KA
Wittbrodt
Joachim
J
Jamrich
Milan
M
D000483:Alleles; D000818:Animals; D005136:Eye Proteins / Q000235:genetics* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000502:physiology*; D019426:Integrases / Q000235:genetics; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D009990:Oryzias / Q000196:embryology / Q000235:genetics; D012160:Retina / Q000196:embryology*
D005136:Eye Proteins; D018398:Homeodomain Proteins; C501274:Rx protein, mouse; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases