title: Interactions of porphyrin covalently attached to poly(methacrylic acid) with liposomal membranes.
abstract: The interactions between the 5-(4-acryloyloxyphenyl)-10,15,20-tritolylporphyrin covalently attached to poly(methacrylic acid) chain (PMA-Po) and phosphatidylcholine liposomes in aqueous solution at different pH values were studied. The binding constants (K(b)) for the liposome- PMA-Po in solutions in the pH range from 6.5 to 9.2 were determined using fluorescence spectroscopy. The binding was found to be efficient. The acid-base properties of the porphyrin chromophores were also studied. Both pK values associated with imine-N protonation of the porphyrin core were found to be 6.4. The quantum yield (Phi(Delta)) of singlet oxygen production by Po in the lipid-PMA-Po system was found to be high (0.88 +/- 0.05).
authors.affiliation: Faculty of Chemistry, Jagiellonian University, 30-060 Kraków, Ingardena 3, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kepczyński
authors.forename: Anna
authors.initials: A
authors.lastname: Karewicz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Górnicki
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D015394:Molecular Structure; D010713:Phosphatidylcholines / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: C521430:5-(4-acryloyloxyphenyl)-10,15,20-tritolylporphyrin; D000179:Acrylates; D008081:Liposomes; D010713:Phosphatidylcholines; D011109:Polymethacrylic Acids; D011166:Porphyrins; D012996:Solutions; D014867:Water; C030613:polymethacrylic acid