[Adjuvant effect of CpG DNA recombinant plasmid on antigen of Cysticercus cellulosae].
In order to prove the adjuvant effect of CpG DNA recombinant plasmid, the total antibodies and their IgG2a subtype induced by antigen of Cysticercus cellulosae, and content of IL-4 and IFN-gamma secreted from splenic cell of mouse immunized were measured. The recombinant plasmids showed an adjuvant effect, and CpG2 was the best adjuvant among the plasmids. It is proved that the CpG DNA possesses a synergistic effect with AI(OH)3 and 206 adjuvant, and is an effective Th1 type adjuvant in mice.
Jing
Zhi-zhong
ZZ
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China.
Meng
Xue-lian
XL
Wang
Pei-ya
PY
Dou
Yong-xi
YX
Li
Hui
H
Luo
Xue-nong
XN
Zheng
Ya-dong
YD
Cai
Xue-peng
XP
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D018899:CpG Islands / Q000235:genetics; D003551:Cysticercosis / Q000097:blood / Q000276:immunology / Q000517:prevention & control; D003552:Cysticercus / Q000276:immunology; D004247:DNA / Q000276:immunology*; D005260:Female; D007114:Immunization; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010957:Plasmids / Q000235:genetics; D011897:Random Allocation; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; C423450:CpG ODN 1585; D007376:Interleukin-2; D007371:Interferon-gamma; D004247:DNA