Taste receptor cells express voltage-dependent potassium channels in a cell age-specific manner.
Two voltage-dependent potassium channels, KCNQ1 and KCNH2, are expressed in the taste buds and were identified as strong candidates involved in the repolarization of taste receptor cells expressing phospholipase C-beta2 and TRPM5 (beta2/M5-TRCs). In cell type-specific expression, KCNQ1 was expressed in most taste bud cells, including beta2/M5-TRCs, whereas KCNH2 was expressed in a subset of beta2/M5-TRCs with no correlation with their taste modality, such as sweet or bitter taste reception. Expression of KCNH2 was restricted to young beta2/M5-TRCs. These results suggest that taste bud cells other than beta2/M5-TRCs are depolarized by some stimuli and also that beta2/M5-TRCs have cell age-dependent molecular mechanisms of repolarization.
Ohmoto
Makoto
M
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Matsumoto
Ichiro
I
Misaka
Takumi
T
Abe
Keiko
K
D000200:Action Potentials / Q000235:genetics / Q000502:physiology*; D000375:Aging / Q000235:genetics / Q000502:physiology*; D000818:Animals; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels / Q000378:metabolism; D005786:Gene Expression Regulation; D007527:Isoenzymes / Q000378:metabolism; D051657:KCNQ1 Potassium Channel / Q000378:metabolism; D054799:Phospholipase C beta; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011984:Sensory Receptor Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D050053:TRPM Cation Channels / Q000378:metabolism; D013650:Taste Buds / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D010738:Type C Phospholipases / Q000378:metabolism
D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; D007527:Isoenzymes; D051657:KCNQ1 Potassium Channel; C000606918:Kcnh2 protein, rat; C496911:Kcnq1 protein, rat; D024642:Potassium Channels, Voltage-Gated; D050053:TRPM Cation Channels; D010738:Type C Phospholipases; D054799:Phospholipase C beta; C518038:Plcb2 protein, rat