title: Protective effects of hyperoxygenated solution preconditioning on intestinal ischemia-reperfusion injury in rabbits.
abstract: BACKGROUND
  The aim of this study was to investigate the protective effects of hyperoxygenated solution (HOS) preconditioning on intestinal ischemia-reperfusion (IR) injury in rabbits.
  
  
  METHODS
  Thirty-two rabbits were randomly divided into four groups as follows: (1) control group in which sham operation was performed (Sham group); (2) sham operation and HOS treatment group (sham+H group); (3) ischemia-reperfusion group (IR group); (4) ischemia-reperfusion and HOS treatment group (H group). Intestinal IR model was produced by clamping superior mesenteric artery with an atraumatic vascular clamp for 1 h, followed by reperfusion for 2 h. Animals in H group received intravenous HOS infusion (20 mL/kg) every day for 5 days before ischemia-reperfusion; animals in the sham+H group received the same amount of HOS before sham operation, and animals in IR group received the same amount of normal saline in the same way. At the end of reperfusion, histopathological changes of intestine were observed, and malondialdehyde (MDA) levels, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities in intestinal tissues were also detected. Intestinal barrier function was assessed by blood d-lactate levels and bacterial translocation (BT).
  
  
  RESULTS
  The H group showed significantly lower MDA levels and higher activities of SOD, CAT, and GSH-Px in the intestinal tissue compared with the IR group. Furthermore, the mean d-lactate levels and incidence of BT in the H group were significantly lower than those in the IR group. Histopathological analysis also indicated that there were significant histological improvements in the H group compared with the IR group.
  
  
  CONCLUSIONS
  HOS preconditioning at an appropriate dose ameliorates the deleterious changes in intestinal mucosal injury and barrier function associated with IR by effectively preventing a decrease in the intestinal antioxidant defense system, which is another simple and effective measure to protect intestine from IR injury.
authors.affiliation: Department of Anesthesiology, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province 710032, China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Chai
authors.forename: Lixian
authors.initials: L
authors.lastname: Xu
authors.forename: Guihe
authors.initials: G
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Lichun
authors.initials: L
authors.lastname: Han
authors.forename: Xude
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply*; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D010100:Oxygen / Q000627:therapeutic use*; D011817:Rabbits; D015427:Reperfusion Injury / Q000517:prevention & control*; D012996:Solutions / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D012996:Solutions; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen