title: Increased large conductance calcium-activated potassium (BK) channel expression accompanied by STREX variant downregulation in the developing mouse CNS.
abstract: BACKGROUND
  Large conductance calcium- and voltage activated potassium (BK) channels are important determinants of neuronal excitability through effects on action potential duration, frequency and synaptic efficacy. The pore- forming subunits are encoded by a single gene, KCNMA1, which undergoes extensive alternative pre mRNA splicing. Different splice variants can confer distinct properties on BK channels. For example, insertion of the 58 amino acid stress-regulated exon (STREX) insert, that is conserved throughout vertebrate evolution, encodes channels with distinct calcium sensitivity and regulation by diverse signalling pathways compared to the insertless (ZERO) variant. Thus, expression of distinct splice variants may allow cells to differentially shape their electrical properties during development. However, whether differential splicing of BK channel variants occurs during development of the mammalian CNS has not been examined.
  
  
  RESULTS
  Using quantitative real-time polymerase chain reaction (RT-PCR) Taqmantrade mark assays, we demonstrate that total BK channel transcripts are up regulated throughout the murine CNS during embryonic and postnatal development with regional variation in transcript levels. This upregulation is associated with a decrease in STREX variant mRNA expression and an upregulation in ZERO variant expression.
  
  
  CONCLUSIONS
  As BK channel splice variants encode channels with distinct functional properties the switch in splicing from the STREX phenotype to ZERO phenotype during embryonic and postnatal CNS development may provide a mechanism to allow BK channels to control distinct functions at different times of mammalian brain development.
authors.affiliation: Centre for Integrative Physiology, School of Biomedical Science, Hugh Robson Building, University of Edinburgh, Edinburgh, Scotland, EH8 9XD, UK. macdonas@tcd.ie
authors.forename: Stephen H-F
authors.initials: SH
authors.lastname: MacDonald
authors.forename: Peter
authors.initials: P
authors.lastname: Ruth
authors.forename: Hans-Guenther
authors.initials: HG
authors.lastname: Knaus
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Shipston
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D002490:Central Nervous System / Q000254:growth & development / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D014644:Genetic Variation; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013116:Spinal Cord / Q000254:growth & development* / Q000378:metabolism
chemical_list: D051036:Large-Conductance Calcium-Activated Potassium Channels; D012333:RNA, Messenger