title: BRSK2 is activated by cyclic AMP-dependent protein kinase A through phosphorylation at Thr260.
abstract: Brain selective kinase 2 (BRSK2) has been identified as a member of AMPK related kinases. LKB1 can phosphorylate the Thr174 of BRSK2, increasing its activity >50-fold. In this study, we identified cAMP-dependent protein kinase A (PKA) as another upstream kinase of BRSK2, which can phosphorylate BRSK2 at Thr260. The association between these two proteins was confirmed by GST pull-down. Furthermore, our study indicated that the kinase activity of BRSK2 can be increased through phosphorylation by PKA.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China.
authors.forename: Zekun
authors.initials: Z
authors.lastname: Guo
authors.forename: Wenwen
authors.initials: W
authors.lastname: Tang
authors.forename: Jian
authors.initials: J
authors.lastname: Yuan
authors.forename: Xinya
authors.initials: X
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Wan
authors.forename: Xiuting
authors.initials: X
authors.lastname: Gu
authors.forename: Kuntian
authors.initials: K
authors.lastname: Luo
authors.forename: Yingli
authors.initials: Y
authors.lastname: Wang
authors.forename: Long
authors.initials: L
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004789:Enzyme Activation; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D016415:Sequence Alignment; D013912:Threonine / Q000378:metabolism*
chemical_list: D013912:Threonine; C507732:Brsk2 protein, human; D017346:Protein Serine-Threonine Kinases; D017868:Cyclic AMP-Dependent Protein Kinases