Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.
A zinc containing metalloprotease, 175 kDa collagenase, purified from adult female Setaria cervi showed strong cross-reactivity with sera from putatively immune (PI) individuals (unpublished observation) and induced cytotoxicity to B. malayi L3 larvae and microfilariae by ADCC mechanism [Srivastava Y, Bhandari YP, Reddy MVR, Harinath BC, Rathaur S. An adult 175 kDa collagenase antigen of Setaria cervi in immunoprophylaxis against Brugia malayi. J Helminth 2004;78:347-52]. These preliminary observations suggested the immunoprotective nature of collagenase. To confirm the vaccine potential of this protease, a vaccine trial was conducted in jirds (Meriones unguiculatus) against human filarial parasite B. malayi. The vaccination resulted into a mean protection level of 75.86% and produced high level of protease neutralizing antibodies. Cytokine analysis in immune jirds sera suggested a mixed Th1/Th2 type cellular immune response whereas ELISA, immunoblotting and enzyme antibody inhibition assay revealed the presence of specific anti-collagenase antibodies. Taken together, all these results suggest that S. cervi 175 kDa collagenase could form the basis of an effective molecular vaccine against human lymphatic filariasis.
Pokharel
Daya Ram
DR
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi-221005, UP, India. drpokharel@rediffmail.com <drpokharel@rediffmail.com>
Rai
Reeta
R
Nandakumar Kodumudi
Krithika
K
Reddy
Maryada Venkata Rami
MV
Rathaur
Sushma
S
D000818:Animals; D017178:Brugia malayi / Q000276:immunology*; D002020:Buffaloes / Q000469:parasitology; D017364:Collagenases / Q000276:immunology*; D004605:Elephantiasis, Filarial / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D005849:Gerbillinae; D008297:Male; D017182:Setaria Nematode / Q000201:enzymology* / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014612:Vaccines / Q000276:immunology / Q000627:therapeutic use*
D014612:Vaccines; D017364:Collagenases