Activation of adenosine receptor on gingival fibroblasts.
CD73 (ecto-5'-nucleotidase) on human gingival fibroblasts plays a role in the regulation of intracellular cAMP levels through the generation of adenosine, which subsequently activates adenosine receptors. In this study, we examined the involvement of ecto-adenosine deaminase, which can be anchored to CD26 on human gingival fibroblasts, in metabolizing adenosine generated by CD73, and thus attenuating adenosine receptor activation. Ecto-adenosine deaminase expression on fibroblasts could be increased by pre-treatment with a lysate of Jurkat cells, a cell line rich in cytoplasmic adenosine deaminase. Interestingly, the cAMP response to adenosine generated from 5'-AMP via CD73 and the ability of 5'-AMP to induce hyaluronan synthase 1 mRNA were significantly decreased by the pre-treatment of fibroblasts with Jurkat cell lysate. This inhibitory effect was reversed by the specific adenosine deaminase inhibitor. These results suggest that ecto-adenosine deaminase metabolizes CD73-generated adenosine and regulates adenosine receptor activation.
Hashikawa
T
T
Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Takedachi
M
M
Terakura
M
M
Yamada
S
S
Thompson
L F
LF
Shimabukuro
Y
Y
Murakami
S
S
D015720:5'-Nucleotidase / Q000378:metabolism; D000241:Adenosine / Q000096:biosynthesis / Q000378:metabolism; D000243:Adenosine Deaminase / Q000096:biosynthesis / Q000378:metabolism*; D000293:Adolescent; D002478:Cells, Cultured; D002648:Child; D000242:Cyclic AMP / Q000378:metabolism; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000382:microbiology; D005881:Gingiva / Q000166:cytology / Q000201:enzymology*; D014453:Glucuronosyltransferase / Q000096:biosynthesis; D006801:Humans; D000076002:Hyaluronan Synthases; D008297:Male; D018047:Receptors, Purinergic P1 / Q000096:biosynthesis*
D018047:Receptors, Purinergic P1; D000242:Cyclic AMP; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases; D015720:5'-Nucleotidase; D018819:Dipeptidyl Peptidase 4; D000243:Adenosine Deaminase; D000241:Adenosine