title: Expressed sequence tags from Madagascar periwinkle (Catharanthus roseus).
abstract: The Madagascar periwinkle (Catharanthus roseus) is well known to produce the chemotherapeutic anticancer agents, vinblastine and vincristine. In spite of its importance, no expressed sequence tag (EST) analysis of this plant has been reported. Two cDNA libraries were generated from RNA isolated from the base part of young leaves and from root tips to select 9,824 random clones for unidirectional sequencing, to yield 3,327 related sequences and 1,696 singletons by cluster analysis. Putative functions of 3,663 clones were assigned, from 5,023 non-redundant ESTs to establish a resource for transcriptome analysis and gene discovery in this medicinal plant.
authors.affiliation: Department of Biological Sciences, Brock University, 500 Glenridge Avenue, St. Catharines, Ont., Canada L2S3A1.
authors.forename: Jun
authors.initials: J
authors.lastname: Murata
authors.forename: Dorothee
authors.initials: D
authors.lastname: Bienzle
authors.forename: Jim E
authors.initials: JE
authors.lastname: Brandle
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Sensen
authors.forename: Vincenzo
authors.initials: V
authors.lastname: De Luca
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D030008:Catharanthus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D020224:Expressed Sequence Tags / Q000737:chemistry; D015723:Gene Library; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000235:genetics; D026121:Indole Alkaloids / Q000378:metabolism; D008780:Methyltransferases / Q000235:genetics; D042282:Microdissection / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D026121:Indole Alkaloids; D014157:Transcription Factors; D003577:Cytochrome P-450 Enzyme System; D008780:Methyltransferases; D005964:Glucosyltransferases