title: Efficient hepatitis C antigen immunohistological staining in sections of normal, cirrhotic and tumoral liver using a new monoclonal antibody directed against serum-derived HCV E2 glycoproteins.
abstract: Detection and localization of Hepatitis C Virus (HCV) in liver tissue is useful for diagnostic purposes as well as to elucidate the mechanisms by which the virus participates in hepatocarcinogenesis. However, so far, a sensitive method for HCV detection at the cellular level is lacking. We describe here the application of a novel antibody, D4.12.9, developed against serum-derived HCV RNA-positive particles, for the detection of E2 proteins by immunohistochemistry in fixed, archived specimens including liver biopsies of HCV-infected patients and surgical specimens of hepatocellular carcinoma. We demonstrate that D4.12.9 is a powerful tool for sensitive and specific detection of HCV, independently of viral genotype. This approach has applications to diagnosis as well as exploratory pathological studies.
authors.affiliation: INSERM U271, 151 Cours Albert Thomas, 69424 Lyon Cedex 03, France.
authors.forename: Oivier
authors.initials: O
authors.lastname: Galy
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Petit
authors.forename: Soumaya
authors.initials: S
authors.lastname: Benjelloun
authors.forename: Philippe
authors.initials: P
authors.lastname: Chevallier
authors.forename: Michelle
authors.initials: M
authors.lastname: Chevallier
authors.forename: Petcharin
authors.initials: P
authors.lastname: Srivatanakul
authors.forename: Anant
authors.initials: A
authors.lastname: Karalak
authors.forename: Christine
authors.initials: C
authors.lastname: Carreira
authors.forename: Nicole
authors.initials: N
authors.lastname: Lyandrat
authors.forename: Abdellah
authors.initials: A
authors.lastname: Essaid
authors.forename: Christian
authors.initials: C
authors.lastname: Trepo
authors.forename: Pierre
authors.initials: P
authors.lastname: Hainaut
authors.forename: Isabelle
authors.initials: I
authors.lastname: Chemin
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D018936:Hepatitis C Antigens / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000821:virology*; D008103:Liver Cirrhosis / Q000175:diagnosis / Q000821:virology*; D008113:Liver Neoplasms / Q000175:diagnosis / Q000821:virology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000097:blood / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D018936:Hepatitis C Antigens; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus