Remodeling of the cone photoreceptor mosaic during metamorphosis of flounder (Pseudopleuronectes americanus).
The retinal cone mosaic of the winter flounder, Pseudopleuronectes americanus, is extensively remodeled during metamorphosis when its visual system shifts from monochromatic to trichromatic. Here we describe the reorganization and re-specification of existing cone subtypes in which larval cones alter their spatial arrangement, morphology, and opsin expression to determine whether mechanisms controlling cell birth, mosaic position, and opsin selection are coordinated or independent. We labeled dividing cells with tritiated ((3)H) thymidine prior to mosaic remodeling to determine whether existing cone photoreceptors change phenotype. We also used in situ hybridization to identify mosaic type and opsin expression in transitional retinas to understand the sequence of transformation. Our data indicate that in the winter flounder retina the choice of new opsin species and the cellular rearrangement of the mosaic proceed independently. The production of the precise cone mosaic arrangement is not due to a stereotyped series of sequential cellular inductions, but rather might be the product of a set of distinct, flexible processes that rely on plasticity in cell phenotype.
Hoke
Kim L
KL
Program in Neurosciences, Stanford University School of Medicine, Stanford, Calif, USA. khoke@psych.stanford.edu
Evans
Barbara I
BI
Fernald
Russell D
RD
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005432:Flounder; D008675:Metamorphosis, Biological / Q000502:physiology*; D017949:Retinal Cone Photoreceptor Cells / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017299:Rod Opsins / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D017299:Rod Opsins; D000469:Alkaline Phosphatase