Characterization of field isolates of infectious laryngotracheitis virus from Ontario.
Five cases of infectious laryngotracheitis (ILT) occurred in the fall of 2004 in the Niagara Peninsula, in Southern Ontario. At about the same time two more cases occurred in Eastern Ontario and one case in South-Western Ontario. We examined, at a molecular level, 10 Ontario ILT virus field isolates from 2004 and early 2005 as well as four ILT vaccine viruses by polymerase chain reaction-restriction fragment length polymorphism analyses of ICP4 and glycoprotein E genes, and partial sequencing of UL47 and glycoprotein G genes. We determined that the five Niagara Peninsula ILT viruses were identical among themselves. They represented an independent cluster of ILT cases and were not related to other cases that occurred during 2004 and early 2005. Viruses isolated during the outbreaks in Eastern and South-Western Ontario could not be differentiated from chicken embryo origin ILT vaccine viruses. Niagara Peninsula isolates were different, at a molecular level, from all four vaccine viruses that were examined and from ILT viruses that had been previously analysed and reported in the literature. Taken together our data indicate that both "wild-type" and vaccine-derived viruses are involved in ILT cases in Ontario.
Ojkic
Davor
D
University of Guelph, Animal Health Laboratory, Canada. dojkic@lsd.uoguelph.ca
Swinton
Janet
J
Vallieres
Marie
M
Martin
Emily
E
Shapiro
Jan
J
Sanei
Babak
B
Binnington
Brian
B
D000818:Animals; D002645:Chickens / Q000821:virology*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D001352:Herpesvirus 1, Gallid / Q000235:genetics* / Q000302:isolation & purification*; D009864:Ontario; D011201:Poultry Diseases / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics
D014759:Viral Envelope Proteins; D054334:Viral Regulatory and Accessory Proteins