Nucleoside phosphocholine amphiphile for in vitro DNA transfection.
A new transfection reagent based on nucleoside phosphocholine amphiphile leading to high transfection efficacy and low cytotoxicity is described. TEM, ethidium bromide displacement assays, agarose gel electrophoresis and SAXS studies support the formation of lipoplexes for the transfection of CHO cells.
Moreau
Louis
L
Faculté des Sciences d'Avignon 33, rue Louis Pasteur, F-84000, Avignon, France.
Barthélémy
Philippe
P
Li
Yougen
Y
Luo
Dan
D
Prata
Carla A H
CA
Grinstaff
Mark W
MW
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D005229:Fatty Acids, Monounsaturated; D017930:Genes, Reporter; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D010767:Phosphorylcholine; D010957:Plasmids; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection / Q000379:methods*; D014529:Uridine / Q000031:analogs & derivatives; D014961:X-Ray Diffraction; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D005229:Fatty Acids, Monounsaturated; D007202:Indicators and Reagents; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins; D010767:Phosphorylcholine; D004247:DNA; D001616:beta-Galactosidase; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane; D014529:Uridine