Biosensing using lipid bilayers suspended on porous silicon.
We demonstrate for the first time the formation of a fluid lipid bilayer membrane on mesoporous silicon substrates for bioapplications. Using fluorescence recovery after photobleaching, the diffusion coefficients for the bilayers supported on oxidized, amino-, and biotin-functionalized mesoporous silicon were determined. The biodetection of a single human umbilical vein endothelial cell was accomplished using confocal microscopy and exploiting Foerster resonance energy transfer effects after the incorporation of RGD covalently linked lipid soluble dyes, with fluorescence donor and acceptor components, within the fluid membrane. A signal response of greater than 100% was achieved via the clustering of RGD peptides binding with areas of high integrin density on the surface of a single cell. These results are a testament to the usefulness of such functional molecular assemblies, based on mobile receptors, mimicking the cell membrane in the development of a new generation of biosensors.
Worsfold
Oliver
O
Frontier Research Division, Fujirebio Inc., 51 Komiya-cho, Hachioji-shi, Tokyo 192-0031, Japan. oworsfold@gmail.com
Voelcker
Nicolas H
NH
Nishiya
Takako
T
D015374:Biosensing Techniques; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D038761:Photobleaching; D016062:Porosity; D012825:Silicon / Q000737:chemistry*; D014471:Umbilical Veins / Q000166:cytology*
D008051:Lipid Bilayers; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D012825:Silicon