Hotspots of transcription factor colocalization in the genome of Drosophila melanogaster.
Regulation of gene expression is a highly complex process that requires the concerted action of many proteins, including sequence-specific transcription factors, cofactors, and chromatin proteins. In higher eukaryotes, the interplay between these proteins and their interactions with the genome still is poorly understood. We systematically mapped the in vivo binding sites of seven transcription factors with diverse physiological functions, five cofactors, and two heterochromatin proteins at approximately 1-kb resolution in a 2.9 Mb region of the Drosophila melanogaster genome. Surprisingly, all tested transcription factors and cofactors show strongly overlapping localization patterns, and the genome contains many "hotspots" that are targeted by all of these proteins. Several control experiments show that the strong overlap is not an artifact of the techniques used. Colocalization hotspots are 1-5 kb in size, spaced on average by approximately 50 kb, and preferentially located in regions of active transcription. We provide evidence that protein-protein interactions play a role in the hotspot association of some transcription factors. Colocalization hotspots constitute a previously uncharacterized type of feature in the genome of Drosophila, and our results provide insights into the general targeting mechanisms of transcription regulators in a higher eukaryote.
Moorman
Celine
C
Department of Molecular Biology, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
Sun
Ling V
LV
Wang
Junbai
J
de Wit
Elzo
E
Talhout
Wendy
W
Ward
Lucas D
LD
Greil
Frauke
F
Lu
Xiang-Jun
XJ
White
Kevin P
KP
Bussemaker
Harmen J
HJ
van Steensel
Bas
B
D000818:Animals; D001665:Binding Sites; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000737:chemistry; D004331:Drosophila melanogaster; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D008957:Models, Genetic; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D002843:Chromatin; D004268:DNA-Binding Proteins; D014157:Transcription Factors