title: Enzymatic determination of total cholesterol in serum by flow injection analysis.
abstract: Several methods are proposed for the enzymatic determination of total cholesterol in serum by flow injection analysis (FIA). The use of the merging-zones mode, with or without halting of the reacting plug in the photometric or fluorimetric detector, has allowed the development of four precise methods for the determination of this analyte; calibration curves were linear for ranges of 0.02-0.2 and 0.005-0.05 gl(-1). These methods have been applied to the determination of cholesterol in serum with excellent results.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Córdoba, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Fernandez-Romero
authors.forename: M D
authors.initials: MD
authors.lastname: Luque de Castro
authors.forename: M
authors.initials: M
authors.lastname: Valcarcel