[Detection of SRPs in injection water of Shenli Oil Field by FISH].
Fluorescence in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes was applied for analyzing the structure of sulfate reducing prokaryotes (SRPs) community in injection water of Shengli Oil Field. Eight probes and their various combinations were used to detect SRPs in the water. Results showed SRPs detected in the water were diverse, which followed in 4 bacterial phyla and 1 archaeal phylum. Total amount of SRPs was 2.86 x 10(4) cells/mL, accounting for 20% of total cells of microorganisms in the water of the Oil Field. Desulfovibrio and Desulfotomaculum cells were about 8.71% (+/- 4.45%) and 12.15% (+/- 3.90%) of the total microbial cells respectively, being dominant in the water. The relative amounts of SRPs belonging to Desulfobacterales and Syntrophobacterales, Thermodesulfobacteriales, and Thermodesulfovibro to total microbial cells in the water were 7.59% (+/- 2.92%), 3.57% (+/- 1.39%) and 2.32% (+/- 0.80%) respectively. In addition, SRPs belonging to Archaeoglobus were also detected with the amount of 4.29% (+/- 1.75%) of total microbial cells, which tells that archeal SRPs are also very important sulfate reducing microorganisms in the injection water of oil field.
Zeng
Jing-hai
JH
ESPC State Key Joint Laboratory, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China. zengjh04@mails.gscas.ac.cn
Wu
Xiao-lei
XL
Zhao
Gui-fang
GF
Qian
Yi
Y
D001105:Archaea / Q000254:growth & development / Q000302:isolation & purification; D045858:Desulfitobacterium / Q000254:growth & development / Q000302:isolation & purification; D045859:Desulfotomaculum / Q000254:growth & development / Q000302:isolation & purification; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence; D010578:Petroleum; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D013431:Sulfates / Q000378:metabolism*; D016999:Sulfur-Reducing Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D014871:Water Microbiology
D010578:Petroleum; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates