Mice congenic for a locus that determines phenotype in the neuroma model of neuropathic pain.
A common strategy for identifying physiological processes responsible for pain is to compare animal strains that show high versus low pain phenotype. However, this approach yields only weak inference because most strains differ from one another at a large number of genetic loci. We undertook a congenic breeding program aimed at transposing the gene(s) that confers dominant (low) pain phenotype in the neuroma model of neuropathic pain from mice of the C58/J strain onto a genetic background that normally expresses high pain phenotype (C3H/HeN). Successful transposition, and the observation of equal numbers of phenotypically high and low offspring in each of 11 generations of selective breeding, provides evidence that the trait is largely controlled by a single gene (or linked cluster) of major effect.
Raber
Pnina
P
Department of Cell and Animal Biology, Institute of Life Sciences and Center for Research on Pain, Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Del Canho
Sonia
S
Darvasi
Ariel
A
Devor
Marshall
M
D000818:Animals; D000831:Animals, Newborn; D002874:Chromosome Mapping; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008815:Mice, Inbred Strains; D009437:Neuralgia / Q000209:etiology* / Q000235:genetics; D009463:Neuroma / Q000150:complications* / Q000235:genetics; D010524:Peripheral Nervous System Neoplasms / Q000150:complications* / Q000235:genetics; D010641:Phenotype; D040641:Quantitative Trait Loci; D012641:Selection, Genetic; D012737:Sex Factors