Detection of genetic homogeneity of Panax notoginseng cultivars by sequencing nuclear 18S rRNA and plastid matK genes.
The nuclear 18S rRNA and chloroplast MATK genes of 18 samples of Panax notoginseng and its processed material Sanqi (Radix Notoginseng) were analyzed. The two genes, regardless of cultivar origin, were found to be identical to genotype R1 and M1, respectively, of the published sequences (GenBank accession no. D85171 and AB027526). This phenomenon implies that the species is highly conserved, which is probably caused by the use of the same strain in cultivation and the lack of active mutation in these two genes.
Zhang
Ying
Y
Institute of Materia Medica, China Academy of Traditional Chinese Medicine, Beijing, PR China.
Zhang
Jin-Chao
JC
Huang
Ming-Hui
MH
Yang
Meng-Su
MS
Cao
Hui
H
D001483:Base Sequence; D017343:Genes, Plant; D005838:Genotype; D008969:Molecular Sequence Data; D009154:Mutation; D005894:Panax / Q000145:classification / Q000235:genetics*; D018087:Plastids / Q000235:genetics*; D015138:RNA, Nuclear / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D015138:RNA, Nuclear; D012337:RNA, Ribosomal, 18S