[Sequence analysis of ITS2 and CO1 genes of Paragonimus harinasutai].
OBJECTIVE
To identify Paragonimus harinasutai from Ninghai, Zhejiang Province, China.


METHODS
Metacercariae were collected from the crabs Sinopotamon chekiangenes in Xixi village of Ninghai County for ITS2 sequence analysis, CO1 sequence analysis and endonuclease BsaHI and StuI analysis by PCR-RFLP. Results The fingerprintings of PCR-RFLP were virtually same to the isolate from Thailand (Nakorn-nayok). The ITS2 sequence with 366 bp and CO1 sequence with 390 bp of the metacercariae collected from Ninghai revealed a nucleotide identity 95.6% and 89.5% respectively to the Thai isolate.


CONCLUSIONS
The study confirmed that Paragonimus harinasutai is present in Ninghai, China, with certain variation on molecular biology in comparison to the Thai isolate.
Qian
Bao-zhen
BZ
Institute of Bio-engineering, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
Sugiyama
H
H
Waikagul
J
J
Zhu
Zhi-hang
ZH
D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D008969:Molecular Sequence Data; D010238:Paragonimus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV