Transient ischemia-induced expression and changes of tyrosine kinase A in the hippocampal dentate gyrus of the gerbil.
The present study examined ischemia-related changes in tyrosine kinase A (trkA) immunoreactivity and its protein content in the dentate gyrus after 5 min of transient forebrain ischemia in gerbils. One day after ischemic insult, cresyl violet-positive polymorphic cells showed ischemic degeneration. The ischemia-induced changes in trkA immunoreactivity were found in the polymorphic layer (PL) and granule cell layer (GCL) of the dentate gyrus. In the sham-operated group, trkA immunoreactivity in the dentate gyrus was very weak. From 30 min after ischemia, trkA immunoreactivity was increased in the dentate gyrus and peaked in the dentate gyrus at 12 h after ischemia-reperfusion. Thereafter, trkA immunoreactivity was decreased time-dependently after ischemia-reperfusion. Four days after ischemic insult, trkA immunoreactivity was similar to that of the sham-operated group. In addition, it was found that ischemia-related changes in trkA protein content were similar to the immunohistochemical changes. These results suggest that the chronological changes of trkA in the dentate gyrus after transient forebrain ischemia may be associated with ischemic damage in polymorphic cells of the dentate gyrus.
Hwang
In Koo
IK
Department of Anatomy, College of Medicine Hallym University, Chunchon, South Korea.
Lee
Hyeon Yong
HY
Yoo
Ki-Yeon
KY
Kim
Dae Ho
DH
Kim
Jung Hwa
JH
Kim
Chang Ho
CH
Lim
Beong Ou
BO
Kang
Tae-Cheon
TC
Bang
Kyung Hwan
KH
Seong
Nak Sul
NS
Lee
Hak Ju
HJ
Kim
Jong Dai
JD
Won
Moo Ho
MH
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D005849:Gerbillinae; D007150:Immunohistochemistry / Q000379:methods; D002546:Ischemic Attack, Transient / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D020534:Parahippocampal Gyrus / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013997:Time Factors
D011505:Protein-Tyrosine Kinases