The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates.
Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same pattern. These findings are not consistent with a prior report. Differences in 23S rRNA gene sequences, between two B. murdochii strains, 56-150 and 155-20, were observed. These results indicate that 23S rRNA PCR-RFLP could be used as an identification method for pathogenic Brachyspira spp. (B. hyodysenteriae and B. pilosicoli) as well as an epidemiological tool for characterizing spirochetes isolated from swine.
Kim
Tae Jung
TJ
School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
Lee
Jae Il
JI
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D004405:Dysentery, Bacillary / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D007723:Korea; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics*; D013144:Spirochaetales / Q000235:genetics* / Q000302:isolation & purification*; D013145:Spirochaetales Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*
D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S