title: Development of capillary electrophoresis  methods for quantitative determination of  taurine in vehicle system and biological  media.
abstract: CE methods have been developed for the determination of taurine in pharmaceutical formulation (microemulsion) and in biological media such as sweat. The CE system with end-column pulsed amperometric detection has been found to be an interesting method in comparison with UV and fluorescence detection for its simplicity and rapidity. A gold-disk electrode of 100 mm diameter was used as the working electrode. The effects of a field decoupler at the end of the capillary, separation voltage, injection and pressure times were investigated. A detection limit of 4 x 10(-5) mol/L was reached using integrated pulsed amperometric detection, a method successfully applied to taurine analysis of the biological samples such as sweat. For taurine analysis of oil-in-water microemulsion, fluorescence detector was the favored method, the detection limit of which was 4 x 10(-11) mol/L.
authors.affiliation: Institute of Pharmaceutical Technology and Biopharmacy, Martin Luther University, Halle-Wittenberg, Germany.
authors.forename: Dayse L P
authors.initials: DL
authors.lastname: da Silva
authors.forename: Hans H
authors.initials: HH
authors.lastname: Rüttinger
authors.forename: Yahia
authors.initials: Y
authors.lastname: Mrestani
authors.forename: Walter F
authors.initials: WF
authors.lastname: Baum
authors.forename: Reinhard H H
authors.initials: RH
authors.lastname: Neubert
mesh_terms: D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D014677:Pharmaceutical Vehicles; D012680:Sensitivity and Specificity; D013542:Sweat / Q000737:chemistry*; D013654:Taurine / Q000032:analysis*
chemical_list: D014677:Pharmaceutical Vehicles; D013654:Taurine; D006046:Gold