title: The CRY box: a second APCcdh1-dependent degron in mammalian cdc20.
abstract: Cdc20 and cdh1 are coactivators of the anaphase-promoting complex (APC). APC(cdc20) is necessary for the metaphase-anaphase transition and, at the end of mitosis, vertebrate cdc20 itself becomes a target for degradation through KEN-box-dependent APC(cdh1) activity. By studying the degradation of fluorescent protein chimaeras in mammalian oocytes and early embryos, we found that cdc20 was degraded through two independent degradation signals (degrons), the KEN box and a newly described CRY box. In both oocytes and G1-stage embryos, the rate of degradation through the CRY box was greater than through the KEN box, although both were mediated by APC(cdh1). Thus, mammalian oocytes and embryos have the capacity to recognize two degrons in cdc20.
authors.affiliation: Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE2 4HH, UK.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Reis
authors.forename: Mark
authors.initials: M
authors.lastname: Levasseur
authors.forename: Heng-Yu
authors.initials: HY
authors.lastname: Chang
authors.forename: David J
authors.initials: DJ
authors.lastname: Elliott
authors.forename: Keith T
authors.initials: KT
authors.lastname: Jones
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D016193:G1 Phase; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D049469:Meiotic Prophase I; D051379:Mice; D009865:Oocytes / Q000378:metabolism; D011489:Protein Denaturation; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D043743:Ubiquitin-Protein Ligase Complexes / Q000378:metabolism*
chemical_list: D064199:Cdc20 Proteins; C577491:Cdc20 protein, mouse; D018797:Cell Cycle Proteins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome