title: The transcript levels of two plant mitochondrial uncoupling protein (pUCP)-related genes are not affected by hyperosmotic stress in durum wheat seedlings showing an increased level of pUCP activity.
abstract: Etiolated early seedlings of durum wheat submitted to moderate and severe salt (NaCl) and osmotic (mannitol) stress showed no relevant increase of both transcript levels of two plant uncoupling protein (pUCP)-related genes and maximal pUCP activity in purified mitochondria (which estimates protein level); contrarily, pUCP functioning due to endogenous free fatty acids strongly increased. These results show that pUCP activation under hyperosmotic stress may be due to modulation of pUCP reaction rather than to an increased protein synthesis. Finally, a properly developed method, based on a single membrane potential measurement, to evaluate both pUCP maximal activity and functioning, is reported.
authors.affiliation: Istituto Sperimentale per la Cerealicoltura-CRA, S.S. 16 Km 675, 71100, Foggia, Italy. trono@iscfoggia.it
authors.forename: Daniela
authors.initials: D
authors.lastname: Trono
authors.forename: Mario
authors.initials: M
authors.lastname: Soccio
authors.forename: Anna M
authors.initials: AM
authors.lastname: Mastrangelo
authors.forename: Vanessa
authors.initials: V
authors.lastname: De Simone
authors.forename: Natale
authors.initials: N
authors.lastname: Di Fonzo
authors.forename: Donato
authors.initials: D
authors.lastname: Pastore
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017920:Antiporters / Q000037:antagonists & inhibitors / Q000378:metabolism; D001278:Atractyloside / Q000031:analogs & derivatives / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005786:Gene Expression Regulation; D017343:Genes, Plant; D006982:Hypertonic Solutions; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009997:Osmotic Pressure; D010085:Oxidative Phosphorylation; D012492:Salts / Q000737:chemistry; D036226:Seedlings / Q000502:physiology*; D014158:Transcription, Genetic; D014908:Triticum / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology*; D000071256:Uncoupling Protein 1
chemical_list: D017920:Antiporters; D005230:Fatty Acids, Nonesterified; D006982:Hypertonic Solutions; D007473:Ion Channels; D024101:Mitochondrial Proteins; D012492:Salts; D000071256:Uncoupling Protein 1; D001278:Atractyloside; D000255:Adenosine Triphosphate; C003853:carboxyatractyloside