An improved mRFP1 adds red to bimolecular fluorescence complementation.
Protein-protein interactions are fundamental to virtually every aspect of cellular functions. Blue, green and yellow bimolecular fluorescence complementation (BiFC) systems based on GFP and its variants allow the investigation of protein-protein interactions in vivo. We have developed the first red BiFC system based on an improved monomeric red fluorescent protein (mRFP1-Q66T), expanding the range of possible applications for BiFC.
Jach
Guido
G
Max Planck Institute for Plant Breeding Research, Department of Plant Developmental Biology, Carl von Linne Weg 10, D-50829 Cologne, Germany. jach@mpiz-koeln.mpg.de
Pesch
Martina
M
Richter
Klaus
K
Frings
Sabine
S
Uhrig
Joachim F
JF
D020869:Gene Expression Profiling / Q000379:methods*; D008164:Luminescent Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein