title: The ability of 67Ga scintigraphy to detect the lesions of Echinococcus multilocularis infection: preliminary results.
abstract: OBJECTIVE
  To assess the ability of 67Ga scintigraphy to detect the lesions of Echinococcus multilocularis (EM) infection.
  
  
  METHODS
  An animal model of EM infection was developed. The infected tissues taken from stock infection were placed into the abdominal cavity of uninfected animals operatively. The success of implantation was controlled 20-25 days after implantation. Five infected and 2 healthy animals were studied. All of the animals were examined by ultrasound before the scintigraphic evaluation. After the injection of 7.4 MBq (200 microCi) 67Ga citrate intravenously, static images from the whole anterior thorax and abdomen were obtained at 24, 48 and 72 hours. Visual and semiquantitative analyses were performed. In semiquantitative analysis, an irregular region of interest was drawn over the thorax as the background, excluding the heart and a second region of interest was drawn over the abdomen, excluding the liver and spleen. Abdomen/ background ratios were calculated using the mean counts.
  
  
  RESULTS
  In the visual evaluation, it was noticed that there was considerably increased 67Ga uptake in the abdomens of the infected animals. In infected animals, mean abdomen/background ratios at 48 and 72 hours (3.76 +/- 1.04, 4.13 +/- 0.72, respectively) were increased compared with mean abdomen/background ratios at 24 hours (2.94 +/- 0.77). These increases in abdomen/background ratios were statistically significant at 72 hours (p = 0.04). Between the infected animals and control group, mean abdomen/background ratios were compared, and statistically significant differences were found in the images obtained at 48 and 72 hours.
  
  
  CONCLUSIONS
  Imaging at 72 hours seems to be more suitable imaging time for the diagnosis of alveolar echinococcosis. 67Ga scintigraphy may successfully demonstrate the lesions of EM infection localized intraperitoneally. The method of 67Ga scintigraphy is useful because it is simple, non-invasive and relatively safe.
authors.affiliation: Department of Parasitology, School of Medicine, Dokuz Eylül University, Izmir, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Inceboz
authors.forename: A
authors.initials: A
authors.lastname: Mavi
authors.forename: G
authors.initials: G
authors.lastname: Capa Kaya
authors.forename: M
authors.initials: M
authors.lastname: Korkmaz
authors.forename: Y
authors.initials: Y
authors.lastname: Goktay
authors.forename: O
authors.initials: O
authors.lastname: Yilmaz
authors.forename: A
authors.initials: A
authors.lastname: Uner
authors.forename: H
authors.initials: H
authors.lastname: Durak
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004444:Echinococcosis, Hepatic / Q000000981:diagnostic imaging* / Q000378:metabolism*; D048210:Echinococcus multilocularis; D005240:Feasibility Studies; D005260:Female; D005708:Gallium / Q000493:pharmacokinetics*; D005849:Gerbillinae; D008657:Metabolic Clearance Rate; D009928:Organ Specificity; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014018:Tissue Distribution
chemical_list: D019275:Radiopharmaceuticals; C034163:gallium chloride; D005708:Gallium