Performance of a reactor containing denitrifying immobilized biomass in removing ethanol and aromatic hydrocarbons (BTEX) in a short operating period.
A horizontal-flow anaerobic immobilized biomass reactor (HAIB) containing denitrifying biomass was evaluated with respect to its ability to remove, separately and in a short operating period (30 days), organic matter, nitrate, and the hydrocarbons benzene (41.4 mg L-1), toluene (27.8 mg L-1), ethylbenzene (31.1 mg L-1), o-xylene (28.5 mg L-1), m-xylene (28.4 mg L-1) and p-xylene (32.1 mg L-1). The purified culture, which was grown in the presence of the specific hydrocarbon, was used as the source of cells to be immobilized in the polyurethane foam. After 30 days of operation, the foam was removed and a new immobilized biomass was grown in the presence of another hydrocarbon. The average hydrocarbon removal efficiency attained was 97%. The organic matter, especially ethanol, was removed with an average efficiency of 83% at a mean influent concentration of 1185.0 mg L-1. A concomitant removal of 97% of nitrate was observed for a mean influent concentration of 423.4 mg L-1. The independent removal of each hydrocarbon demonstrated that these contaminants can be biodegraded separately, without the need for a compound to be the primary substrate for the degradation of another. This study proposes the application of the system for treatment of areas contaminated with these compounds, with substitution and formation of a biofilm in a 30-day period.
Gusmão
Valquiria Ribeiro
VR
Laboratory of Biological Processes, Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo (USP), Av. Trabalhador São-carlense 400, São Carlos 13566-590, SP, Brazil. vgusmao@sc.usp.br
Chinalia
Fábio Alexandre
FA
Sakamoto
Isabel Kimiko
IK
Varesche
Maria Bernadete Amâncio
MB
D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D018914:Cells, Immobilized / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D010230:Paracoccus / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D018508:Water Purification / Q000295:instrumentation* / Q000379:methods*
D006841:Hydrocarbons, Aromatic; D000431:Ethanol