title: hSef co-localizes and interacts with Ras in the inhibition of Ras/MAPK signaling pathway.
abstract: To study the mechanism of the inhibitory effects of Sef (similar expression to fgf genes) on Ras/mitogen-activated protein kinase (MAPK) signaling pathway, we observed cellular localization of this protein. Immunofluorescent staining results show that Sef locates in the vesicles of the cytoplasm without bFGF treatment but co-localizes with Ras on the plasma membrane (PM) in response to bFGF stimulation. The coimmunoprecipitation assay demonstrates that Sef interacts with Ras or RasG12V, respectively. We observed that Sef inhibited FGF induced, but not RasG12V mediated, signal transduction. We propose that Sef interacted with Ras in the inhibition of Ras/MAPK signaling pathway.
authors.affiliation: Tsinghua Institute of Genome Research, Department of Biological Sciences and Biotechnology, and School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Ren
authors.forename: Long
authors.initials: L
authors.lastname: Cheng
authors.forename: Zhili
authors.initials: Z
authors.lastname: Rong
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Li
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Li
authors.forename: Hongge
authors.initials: H
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Chang
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D047468:Immunoprecipitation; D020935:MAP Kinase Signaling System / Q000502:physiology*; D018123:Receptors, Interleukin / Q000502:physiology*; D018631:ras Proteins / Q000502:physiology*
chemical_list: C477526:IL17RD protein, human; D018123:Receptors, Interleukin; D016222:Fibroblast Growth Factor 2; D018631:ras Proteins