title: Acetylcholinesterase mutation in an insecticide-resistant population of the codling moth Cydia pomonella (L.).
abstract: Two strains of Cydia pomonella (L.) (Lepidoptera: Tortricidae) were selected in the lab by exposure to increasing concentrations of diflubenzuron (Rdfb strain) or azinphos-methyl (Raz strain). Insecticide bioassays showed that the adults of the Rdfb strain exhibited a 2.6-fold and a 7.7-fold resistance ratio to azinphos-methyl and carbaryl, respectively compared to a susceptible strain (S) whereas the adults of the Raz strain exhibited a 6.7-fold resistance ratio to azinphos-methyl and a 130-fold resistance ratio to carbaryl. In the Raz strain, a target site resistance mechanism was suggested by the inhibition of acetylcholinesterase (AChE) activity. In fact the ki values did not discriminate the S and Rdfb strains, while the Raz strain exhibited a 1.7-fold and a 14-fold increase in ki value compared to the S strain for azinphos-methyl oxon and carbaryl, respectively. To verify this hypothesis, two cloned AChE cDNAs sequences (named cydpom-ace2 e cydpom-ace1) were compared between the susceptible and the resistant strains. No difference in the deduced amino acid sequence was found in cydpom-ace2 (orthologous to the Drosophila melanogaster AChE). In the putative cydpom-ace1 (paralogous to the Drosophila AChE), a single amino acid substitution F399V was exclusively present in the Raz strain. The F399 lined the active site of the enzyme and the F399V substitution likely could influence the accessibility of different types of inhibitors to the catalytic site of the insensitive cydpom-ace1.
authors.affiliation: Department of Agricultural Science, University of Modena and Reggio Emilia, Reggio Emilia, Via JFK Kennedy 17/19, RE 42100, Italy. stcass@unimore.it
authors.forename: Stefano
authors.initials: S
authors.lastname: Cassanelli
authors.forename: Maritza
authors.initials: M
authors.lastname: Reyes
authors.forename: Magali
authors.initials: M
authors.lastname: Rault
authors.forename: Gian
authors.initials: G
authors.lastname: Carlo Manicardi
authors.forename: Benoît
authors.initials: B
authors.lastname: Sauphanor
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D005784:Gene Amplification; D005838:Genotype; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D017422:Sequence Analysis, DNA
chemical_list: D007306:Insecticides; D000110:Acetylcholinesterase