Interleukin 6 and 8 levels in plasma and fibroblast cultures in psoriasis.
Fibroblasts have been implicated in psoriatic inflammatory processes. The aim of the study was to evaluate soluble interleukin 2 receptor (sIL-2R), interleukin 6 (IL-6), and interleukin 8 (IL-8) plasma levels in psoriatic patients and IL-6 and IL-8 levels in fibroblast culture supernatants. Cytokines levels in plasma and supernatants were measured by ELISA. Plasma sIL-2R, IL-6, and IL-8 levels were higher before the treatment in comparison to healthy controls (P < 0.001) and decreased after treatment. Fibroblasts from healthy controls, psoriatic lesional skin, and noninvolved psoriatic skin, when stimulated with tumor necrosis factor alpha, released considerable amounts of IL-6 and IL-8. No significant difference between healthy controls and psoriatic fibroblasts was observed. Monitoring plasma sIL-2R levels could be employed as a reliable method of psoriasis activity. IL-8 and IL-6 plasma levels seem to reflect psoriasis activity, and treatment response, respectively. Fibroblasts are not a major source of increased IL-6 and IL-8 production in psoriasis.
Zalewska
Anna
A
Department of Dermatology and Veneriology, Medical University of Łódź, 5 Krziemieniecka Street, 94-017 Łódź, Poland.
Głowacka
Ewa
E
Wyczółkowska
Janina
J
Tchórzewski
Henryk
H
Narbutt
Joanna
J
Sysa-Jedrzejowska
Anna
A
D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation; D015850:Interleukin-6 / Q000097:blood* / Q000378:metabolism; D016209:Interleukin-8 / Q000097:blood* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000097:blood* / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000097:blood / Q000378:metabolism
D015850:Interleukin-6; D016209:Interleukin-8; D015375:Receptors, Interleukin-2