[Study on the application of chemometrics methods in chemical characteristic fingerprinting of Fritillaria].
A method for determination of eleven samples in Fritillaria by HPLC with UV detection at 215 nm was described. The column was used a XDP-C18, 4.6 x 250 mm, 5 microm, and the mobile phase was methanol-water (volume: volume = 70: 30, to be added 7.5 mmol/L SDS, pH 4.5 +/- 0.1). Important parameters with chromatograms of eleven samples were obtained and crusted. The results indicated that it is similar between clustering analysis and taxonomy of Fritillaria. Identification of Fritillaria and establishment of the chemical characteristic fingerprinting can used the methods.
Li
Yu-Feng
YF
Department of Pharmaceutical Engineering, Xihua University, Chengdu 610000, China.
Yan
Fang
F
Tang
Lin
L
Chen
Fang
F
D000470:Alkaloids / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016000:Cluster Analysis; D031410:Fritillaria / Q000737:chemistry* / Q000145:classification; D010946:Plants, Medicinal / Q000737:chemistry*
D000470:Alkaloids