title: Evaluation of sample work-up methods and internal standards for the determination of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in brain by HPLC with electrochemical detection.
abstract: A high-performance liquid chromatographic method with electrochemical detection is described for the determination of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in brain. Results of studies on sample work-up methods and on the use of various internal standards are reported. The reproducibility of determination of 5-HT and 5-HIAA in two regions of rabbit brain has been evaluated.
authors.affiliation: Department of Biochemistry, University of Limburg, B-3610 Diepenbeek, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Duchateau
authors.forename: H
authors.initials: H
authors.lastname: Martens
authors.forename: H
authors.initials: H
authors.lastname: Teuchy
authors.forename: A
authors.initials: A
authors.lastname: Schoups
authors.forename: M
authors.initials: M
authors.lastname: Claeys