Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression.
Multiple myeloma (MM) is a product of interactions between tumor plasma cells and multiple cell types native to the bone marrow (BM). We have used antibody array technology to examine the proteins produced by BM stromal cells in response to stimulation by BM taken from patients diagnosed with monoclonal gammopathy of undetermined significance (MGUS), smoldering multiple myeloma (SMM), and MM. We observed increased production of the chemokine IL-8 by stromal cells co-cultured with supernatants from bone marrow cells of patients with active myeloma. IL-8 production is correlated with active disease and is dependent upon IL-1beta and NF-kappaB signaling. Consistent with the pro-angiogenic activity of IL-8, increased BM microvessel density (MVD) correlated with stimulation of stromal cell IL-8 production. In addition, the majority of MM cell lines and MM patient plasma cells were found to express IL-8 receptors CXCR1 and CXCR2. We conclude that stromal cell IL-8 production parallels MM disease activity, is IL-1beta induced, and correlates with bone marrow angiogenesis.
Kline
Michael
M
Division of Hematology and Internal Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Donovan
Kathleen
K
Wellik
Linda
L
Lust
Christopher
C
Jin
Wendy
W
Moon-Tasson
Laurie
L
Xiong
Yuning
Y
Witzig
Thomas E
TE
Kumar
Shaji
S
Rajkumar
S Vincent
SV
Lust
John A
JA
D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D018925:Chemokines / Q000378:metabolism*; D018450:Disease Progression; D005434:Flow Cytometry; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism*; D046228:Microarray Analysis; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism; D009389:Neovascularization, Pathologic; D010265:Paraproteinemias / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D053700:Receptors, Interleukin-8 / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism
D018925:Chemokines; D053583:Interleukin-1beta; D016209:Interleukin-8; D016328:NF-kappa B; D053700:Receptors, Interleukin-8