Halothane affects focal adhesion proteins in the A 549 cells.
Halothane is a volatile anaesthetic, which is known to induce alterations in cellular plasma membranes, modulating the physical state of the membrane lipids and/or interacting directly with membrane-bound proteins, such as integrin receptors. Integrin-mediated cell adhesion is a general property of eukaryotic cells, which is closely related to cell viability. Our previous investigations showed that halothane is toxic for A 549 lung carcinoma cells when applied at physiologically relevant concentrations and causes inhibition of adhesion to collagen IV. The present study is focused on the mechanisms underlying halothane toxicity. Our results imply that physiologically relevant concentrations of halothane disrupt focal adhesion contacts in A 549 cells, which is accompanied with suppression of focal adhesion kinase activity and paxillin phosphorylation, and not with proteolytic changes or inhibition of vinculin and paxillin expression.We suggest that at least one of the toxic effects of halothane is due to a decreased phosphorylation of the focal contact proteins.
Valtcheva-Sarker
Ralitca
R
Faculty of Biology, Department of Cytology, Histology and Embryology, Sofia University, St. Kl. Ohridski 8, Dragan Tzankov str., 1164, Sofia, Bulgaria.
Stephanova
Elena
E
Hristova
Kamelia
K
Altankov
George
G
Momchilova
Albena
A
Pankov
Roumen
R
D002448:Cell Adhesion / Q000187:drug effects; D024141:Collagen Type IV / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D022001:Focal Adhesions / Q000187:drug effects*; D006221:Halothane / Q000633:toxicity*; D006801:Humans; D051419:Paxillin / Q000378:metabolism*; D019000:Phosphotyrosine / Q000378:metabolism; D014407:Tumor Cells, Cultured; D016596:Vinculin / Q000378:metabolism*
D024141:Collagen Type IV; D051419:Paxillin; D016596:Vinculin; D019000:Phosphotyrosine; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006221:Halothane