title: Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.
abstract: A zinc containing metalloprotease, 175 kDa collagenase, purified from adult female Setaria cervi showed strong cross-reactivity with sera from putatively immune (PI) individuals (unpublished observation) and induced cytotoxicity to B. malayi L3 larvae and microfilariae by ADCC mechanism [Srivastava Y, Bhandari YP, Reddy MVR, Harinath BC, Rathaur S. An adult 175 kDa collagenase antigen of Setaria cervi in immunoprophylaxis against Brugia malayi. J Helminth 2004;78:347-52]. These preliminary observations suggested the immunoprotective nature of collagenase. To confirm the vaccine potential of this protease, a vaccine trial was conducted in jirds (Meriones unguiculatus) against human filarial parasite B. malayi. The vaccination resulted into a mean protection level of 75.86% and produced high level of protease neutralizing antibodies. Cytokine analysis in immune jirds sera suggested a mixed Th1/Th2 type cellular immune response whereas ELISA, immunoblotting and enzyme antibody inhibition assay revealed the presence of specific anti-collagenase antibodies. Taken together, all these results suggest that S. cervi 175 kDa collagenase could form the basis of an effective molecular vaccine against human lymphatic filariasis.
authors.affiliation: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi-221005, UP, India. drpokharel@rediffmail.com <drpokharel@rediffmail.com>
authors.forename: Daya Ram
authors.initials: DR
authors.lastname: Pokharel
authors.forename: Reeta
authors.initials: R
authors.lastname: Rai
authors.forename: Krithika
authors.initials: K
authors.lastname: Nandakumar Kodumudi
authors.forename: Maryada Venkata Rami
authors.initials: MV
authors.lastname: Reddy
authors.forename: Sushma
authors.initials: S
authors.lastname: Rathaur
mesh_terms: D000818:Animals; D017178:Brugia malayi / Q000276:immunology*; D002020:Buffaloes / Q000469:parasitology; D017364:Collagenases / Q000276:immunology*; D004605:Elephantiasis, Filarial / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D005849:Gerbillinae; D008297:Male; D017182:Setaria Nematode / Q000201:enzymology* / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014612:Vaccines / Q000276:immunology / Q000627:therapeutic use*
chemical_list: D014612:Vaccines; D017364:Collagenases