Local hydration environments of amino acids and dipeptides studied by X-ray spectroscopy of liquid microjets.
The nitrogen K-edge spectra of aqueous proline and diglycine solutions have been measured by total electron yield near-edge X-ray absorption fine structure (NEXAFS) spectroscopy at neutral and high pH. All observed spectral features have been assigned by comparison to the recently reported spectrum of aqueous glycine and calculated spectra of isolated amino acids and hydrated amino acid clusters. The sharp preedge resonances at 401.3 and 402.6 eV observed in the spectrum of anionic glycine indicate that the nitrogen terminus is in an "acceptor-only" configuration, wherein neither amine proton is involved in hydrogen bonding to the solvent, at high pH. The analogous 1s --> sigma(NH) preedge transitions are absent in the NEXAFS spectrum of anionic proline, implying that the acceptor-only conformation observed in anionic glycine arises from steric shielding induced by free rotation of the amine terminus about the glycine CN bond. Anionic diglycine solutions exhibit a broadened 1s --> pi(CN) resonance at 401.2 eV and a broad shoulder resonance at 403 eV, also suggesting the presence of an acceptor-only species. Although this assignment is not as unambiguous as for glycine, it implies that the nitrogen terminus of most proteins is capable of existing in an acceptor-only conformation at high pH. The NEXAFS spectrum of zwitterionic lysine solution was also measured, exhibiting features similar to those of both anionic and zwitterionic glycine, and leading us to conclude that the alpha amine group is present in an acceptor-only configuration, while the end of the butylammonium side chain is fully solvated.
Messer
B M
BM
Department of Chemistry, University of California, Berkeley, California 94720, USA.
Cappa
C D
CD
Smith
J D
JD
Drisdell
W S
WS
Schwartz
C P
CP
Cohen
R C
RC
Saykally
R J
RJ
D005998:Glycine / Q000737:chemistry*; D006033:Glycylglycine / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000737:chemistry*; D008968:Molecular Conformation; D015394:Molecular Structure; D011392:Proline / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D013057:Spectrum Analysis / Q000379:methods*; D014867:Water / Q000737:chemistry; D014965:X-Rays
D012996:Solutions; D014867:Water; D006033:Glycylglycine; D011392:Proline; D008239:Lysine; D005998:Glycine