Development of in vitro immunization in murine and human hybridoma technology.
The development of in vitro immunization in murine and human hybridoma technology is reviewed. Special consideration is given to: technical aspects of in vitro immunization of mouse B cells; quality of antibodies produced by in vitro immunization; advantages of murine in vitro immunization; technical aspects of in vitro immunization of human B cells; and the advantages of human in vitro immunization and human monoclonal antibodies. Future developments likely to be made in this area of immunotechnology are outlined.
Borrebaeck
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Department of Biotechnology, University of Lund, P.O. Box 124, S-221 00 Lund, Sweden.