title: [Immunohistochemical analysis of angiogenesis in invasive ductal breast carcinoma with correlations to clinicopathological factor].
abstract: OBJECTIVE
  Angiogenesis is the formation of new vessels from preexisting ones. The aim of our study was to determine the relevance of tumor-induced angiogenesis, its correlation with some of the commonly used clinical, pathological factors and the recent biological indicators, and metastatic potential of the tumor in a series of 120 patients with invasive ductal carcinoma of the breast.
  
  
  METHODS
  The identification of microvessels was performed immunohistochemically with factor VIII-related antigen. The microvessel count (MVC) was assessed at the invasive front of each carcinoma. The cases were divided into high-and low-microvessel density groups according to an average number of microvessels found in the multiple fields of the most vessel-dense part of each tumor. The nuclear immunohistochemical staining for hormone receptors, and the p53, and the membranous staining for c-erbB-2 were evalueted.
  
  
  RESULTS
  There were significant correlations between a high MVC and a large tumor size, high histological grade, and c-erbB-2 protein overexpression. There was no association between tumor angiogenesis, as assessed by the MVC, and the hormone receptors status, and the p53 protein expression. In the cases with a high MVC, there was a significant number of tumors with lymph node metastases.
  
  
  CONCLUSIONS
  Our findings showed that a high MVC might point out an aggressive tumor phenotype. This might help to stratify patients for an appropriate therapy on an individual basis and, thus, offer the possibility of a more effectively tailored treatment program.
authors.affiliation: Institut za onkologiju Sremska Kamenica, Klinika za operativnu onkologiju, Odeljenje za patologiju, Novi Sad. kapicl@Eunet.yu
authors.forename: Tatjana
authors.initials: T
authors.lastname: Ivković-Kapicl
authors.forename: Slavica
authors.initials: S
authors.lastname: Knelević-Usaj
authors.forename: Milana
authors.initials: M
authors.lastname: Panjković
authors.forename: Katarina
authors.initials: K
authors.lastname: Mastilović
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D001943:Breast Neoplasms / Q000098:blood supply* / Q000737:chemistry / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000098:blood supply* / Q000737:chemistry / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor