title: Nucleoside phosphocholine amphiphile for in vitro DNA transfection.
abstract: A new transfection reagent based on nucleoside phosphocholine amphiphile leading to high transfection efficacy and low cytotoxicity is described. TEM, ethidium bromide displacement assays, agarose gel electrophoresis and SAXS studies support the formation of lipoplexes for the transfection of CHO cells.
authors.affiliation: Faculté des Sciences d'Avignon 33, rue Louis Pasteur, F-84000, Avignon, France.
authors.forename: Louis
authors.initials: L
authors.lastname: Moreau
authors.forename: Philippe
authors.initials: P
authors.lastname: Barthélémy
authors.forename: Yougen
authors.initials: Y
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
authors.forename: Carla A H
authors.initials: CA
authors.lastname: Prata
authors.forename: Mark W
authors.initials: MW
authors.lastname: Grinstaff
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D005229:Fatty Acids, Monounsaturated; D017930:Genes, Reporter; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D010767:Phosphorylcholine; D010957:Plasmids; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection / Q000379:methods*; D014529:Uridine / Q000031:analogs & derivatives; D014961:X-Ray Diffraction; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D005229:Fatty Acids, Monounsaturated; D007202:Indicators and Reagents; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins; D010767:Phosphorylcholine; D004247:DNA; D001616:beta-Galactosidase; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane; D014529:Uridine