title: Platelet derived growth factor regulates ABCA1 expression in vascular smooth muscle cells.
abstract: The ATP-binding cassette transporter A1 (ABCA1) regulates lipid efflux from peripheral cells to High-density lipoprotein. The platelet-derived growth factor (PDGF) is a potent mitogen that enables vascular smooth muscle cells to participate in atherosclerosis. In this report, we showed that PDGF suppressed endogenous expression of ABCA1 in cultured vascular smooth muscle cells. Exposure of CRL-208 cells to PDGF elicited a rapid phosphorylation of a kinase downstream from PI3-K, Akt. The constitutively active form of both p110, a subunit of PI3-K, and Akt inhibited activity of the ABCA1 promoter. In conclusion, PI3-K-Akt pathways participate in PDGF-suppression of ABCA1 expression.
authors.affiliation: Department of Internal Medicine, Division of Endocrinology and Metabolism, Faculty of Medicine, Kagawa University, Kita-gun, Kagawa, Japan.
authors.forename: Sachi
authors.initials: S
authors.lastname: Nagao
authors.forename: Koji
authors.initials: K
authors.lastname: Murao
authors.forename: Hitomi
authors.initials: H
authors.lastname: Imachi
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Cao
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.forename: Junhua
authors.initials: J
authors.lastname: Li
authors.forename: Kensuke
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Takamasa
authors.initials: T
authors.lastname: Nishiuchi
authors.forename: Rania A M
authors.initials: RA
authors.lastname: Ahmed
authors.forename: Norman C W
authors.initials: NC
authors.lastname: Wong
authors.forename: Kazumitsu
authors.initials: K
authors.lastname: Ueda
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ishida
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D010982:Platelet-Derived Growth Factor; D002784:Cholesterol; D051057:Proto-Oncogene Proteins c-akt