title: Elevated levels of oxidative DNA damage in serum and myocardium of patients with heart failure.
abstract: BACKGROUND
  Oxidative stress has been implicated in the pathogenesis of chronic heart failure. The present study investigated whether the levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage, were elevated in the serum and myocardium of patients with dilated cardiomyopathy (DCM), and furthermore whether carvedilol, a vasodilating beta-blocker with antioxidant activity, could reduce the levels.
  
  
  RESULTS
  Serum levels of 8-OHdG were measured by enzyme immunoassay in 56 patients with DCM and in 20 control subjects. DCM patients had significantly elevated serum levels of 8-OHdG compared with control subjects. Endomyocardial biopsy samples obtained from 12 DCM patients and 5 control subjects with normal cardiac function were studied immunohistochemically for the expression of 8-OHdG. Positive 8-OHdG staining was found in the nuclei of cardiomyocytes from DCM patients but not in those from control subjects. After treatment with carvedilol, the serum levels of 8-OHdG in DCM patients significantly decreased by 19%, together with amelioration of heart failure.
  
  
  CONCLUSIONS
  Levels of 8-OHdG are elevated in the serum and myocardium of patients with heart failure. Treatment with carvedilol might be effective for decreasing the oxidative DNA damage.
authors.affiliation: Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, and Division of Cardiology, National Hospital Organization Okayama-Medical Center, Japan. y-kono@gk9.so-net.ne.jp
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kono
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hideo
authors.initials: H
authors.lastname: Kimura
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nishii
authors.forename: Atsuyuki
authors.initials: A
authors.lastname: Watanabe
authors.forename: Kimikazu
authors.initials: K
authors.lastname: Banba
authors.forename: Aya
authors.initials: A
authors.lastname: Miura
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagase
authors.forename: Satoru
authors.initials: S
authors.lastname: Sakuragi
authors.forename: Kengo Fukushima
authors.initials: KF
authors.lastname: Kusano
authors.forename: Hiromi
authors.initials: H
authors.lastname: Matsubara
authors.forename: Tohru
authors.initials: T
authors.lastname: Ohe
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D002227:Carbazoles / Q000494:pharmacology; D002303:Cardiac Output, Low / Q000097:blood* / Q000188:drug therapy / Q000503:physiopathology*; D000077261:Carvedilol; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D018384:Oxidative Stress; D011412:Propanolamines / Q000494:pharmacology
chemical_list: D000319:Adrenergic beta-Antagonists; D015415:Biomarkers; D002227:Carbazoles; D011412:Propanolamines; D000077261:Carvedilol; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine