The effect of surfactants on the high-performance liquid chromatography of anthracyclines.
A rapid, reversed-phase high-performance liquid chromatographic method is described for the determination of the antineoplastic drug, doxorubicin (Adriamycin), and its major metabolites in plasma. The addition of anionic, cationic or non-ionic surfactants was found to reduce k'; the mechanisms of this finding are discussed and the performance of an optimized method using a non-ionic surfactant, Brij-35, is described for plasma samples.
Watson
I D
ID
Drug Investigation Unit, Department of Biochemistry, Royal Infirmary, Glasgow G4 0SF, UK.
Stewart
M J
MJ
Farid
Y Y
YY