Outer membrane vesicle production by Escherichia coli is independent of membrane instability.
It has been long noted that gram-negative bacteria produce outer membrane vesicles, and recent data demonstrate that vesicles released by pathogenic strains can transmit virulence factors to host cells. However, the mechanism of vesicle release has remained undetermined. This genetic study addresses whether these structures are merely a result of membrane instability or are formed by a more directed process. To elucidate the regulatory mechanisms and physiological basis of vesiculation, we conducted a screen in Escherichia coli to identify gene disruptions that caused vesicle over- or underproduction. Only a few low-vesiculation mutants and no null mutants were recovered, suggesting that vesiculation may be a fundamental characteristic of gram-negative bacterial growth. Gene disruptions were identified that caused differences in vesicle production ranging from a 5-fold decrease to a 200-fold increase relative to wild-type levels. These disruptions included loci governing outer membrane components and peptidoglycan synthesis as well as the sigma(E) cell envelope stress response. Mutations causing vesicle overproduction did not result in upregulation of the ompC gene encoding a major outer membrane protein. Detergent sensitivity, leakiness, and growth characteristics of the novel vesiculation mutant strains did not correlate with vesiculation levels, demonstrating that vesicle production is not predictive of envelope instability.
McBroom
Amanda J
AJ
Department of Biochemistry, Duke University Medical Center, Box 3711, Durham, NC 27710, USA.
Johnson
Alexandra P
AP
Vemulapalli
Sreekanth
S
Kuehn
Meta J
MJ
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000378:metabolism; D017353:Gene Deletion; D018272:Porins / Q000378:metabolism; D012808:Sigma Factor / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D022161:Transport Vesicles / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C113724:OmpC protein; D018272:Porins; D012808:Sigma Factor; D014157:Transcription Factors; C063202:sporulation-specific sigma factors; D012321:DNA-Directed RNA Polymerases