title: [DNA genotyping of oral epithelial cells by laser capture microdissection].
abstract: OBJECTIVE
  The STR genotypping of trace oral epithelial cells which are microdissected by laser capture microdissection system (LCM) is explored.
  
  
  METHODS
  The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures.
  
  
  RESULTS
  DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed.
  
  
  CONCLUSIONS
  It is viable in genotyping of trace oral epithelial cells by Laser Capture Microdissection as a new technology of seperating single cell.
authors.affiliation: Shanghai Institute of Forensic Science, Shanghai, 200083, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Gu
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Lian-Kang
authors.initials: LK
authors.lastname: Chen
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Zhen
authors.forename: Li
authors.initials: L
authors.lastname: Cheng
authors.forename: Huai-Gu
authors.initials: HG
authors.lastname: Zhou
mesh_terms: D002469:Cell Separation / Q000379:methods; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004847:Epithelial Cells; D005838:Genotype; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000295:instrumentation / Q000379:methods*; D009055:Mouth / Q000166:cytology*; D020080:Tandem Repeat Sequences
chemical_list: D004247:DNA