Chromatography in analytical toxicology--state of the art and future perspectives.
The aims of systematic toxicological analysis are considered with respect to the principal techniques available for quantitative and qualitative purposes. The use of thin-layer (TLC), gas-liquid (GLC), capillary GLC and high-performance liquid chromatography (HPLC) is discussed, with special reference to the need for standardized systems for toxicological analysis. The selectivity of correlations presented as mean list length values, derived from archives of Kováts retention indices in GLC and of Rf-values in up to eight TLC systems, is illustrated by some typical examples. The dependence of Kováts retention indices on column packing materials and on load capacity in CGLC is discussed and the particular difficulties of comparable standard systems in HPLC are considered. Conclusions are drawn concerning the established 'workhorse' techniques, such as TLC and GLC. The need to adapt and standardize other powerful analytical methods, such as HPLC, MS and GLC-MS, is examined with regard to the requirements of toxicological analysis.
de Zeeuw
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Department of Toxicology, State University, A. Deusinglaan 2, 9713 AW Groningen, The Netherlands.