title: [Effects and mechanism of ShenQi compound recipe on inflammation maker in GK rats].
abstract: OBJECTIVE
  To explore the effects and mechanism of ShenQi Compound Recipe on inflammation maker of type 2 diabetes mellitus in GK rats.
  
  
  METHODS
  Rats were ranodmly divided into Model group, Ramipril group (positive control, 1 mg/kg x d), SQCR low dosage (0.72 g/kg x d), SQCR high dosage group (2.88 g/kg x d) and Wistar control group. Each group was administrated correspondent substance respectively for 32 days. Determined C-reactive protein (CRP) by ELISA and tumour necrosis factor (TNF)-alpha by radioimmunassay. The mRNA expression of nuclear factor (NF)-kappaB p65 in aorta was determined by real time RT-PCR, and activation of it using immunohistochemistry staining.
  
  
  RESULTS
  Concentrations of CRP and TNF-alpha in serum and the expression of mRNA and activation of NF-kappaB were all decreased in SQCR low and high dosage groups compared with model group (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  These results suggest that SQCR can decrease the level of inflammation maker in serum, which may be resulted from reducing the mRNA expression and activation of NF-kappaB.
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Zhang
authors.forename: Shi-Wei
authors.initials: SW
authors.lastname: Chen
authors.forename: Chun-Guang
authors.initials: CG
authors.lastname: Xie
authors.forename: Yi-Qiang
authors.initials: YQ
authors.lastname: Xie
authors.forename: Xi-Fang
authors.initials: XF
authors.lastname: Deng
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism; D027885:Astragalus propinquus / Q000737:chemistry; D002097:C-Reactive Protein / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000188:drug therapy; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis; D011863:Radioimmunoassay; D017257:Ramipril / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein; D017257:Ramipril