Studies of interaction between a new synthesized minor-groove targeting artificial nuclease and DNA.
Nuclease plays an important role in molecular biology, such as DNA sequencing. Synthetic polyamide conjugates can be considered as new tool in the selective inhibition of gene expression and as potential drugs in anticancer or antiviral chemotherapy. In this paper, a new synthesized minor-groove targeting artificial nuclease, oligopyrrol-containing peptide, was reported. It was found that this new compound can bind DNA in AT-riched minor groove with high affinity and site specificity. DNA binding behavior was determined by UV-vis and circular dichroism (CD) methods. It was indicated that compound 6 can enhance the Tm of oligomer DNA from 51.8 to 63.5 degrees C and possesses large binding constant (Kb=8.83x10(4)L/mol).
Yin
Qiang
Q
The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, PR China. yinq03@mails.tsinghua.edu.cn
Zhang
Zhen
Z
Zhao
Yu-Fen
YF
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides; D004247:DNA; C427203:calf thymus DNA; D004720:Endonucleases