title: Biocompatibility of various formula root filling materials for primary teeth.
abstract: The aim of this study was to compare the effects of different materials used in primary root canal fillings on the cell viability of human osteosarcoma cell lines. The experimental group contained six different types of root canal filling materials, including zinc oxide (ZnO) + eugenol + formocresol (FC), Ca(OH)(2) + FC, Ca(OH)(2) + Iodoform, Ca(OH)(2) + Iodoform + camphorated parachlorophenol (CPC), Ca(OH)(2) + CPC, and Vitapex. Cell viability tests were performed using tetrazolium bromide colorimetric (MTT) assay on human osteosacorma cell lines (U2OS). The results were analyzed using one-way analysis of variance (ANOVA) and Student-Newman-Keul's test with p < 0.05 showed statistical differences. The ZnO + eugenol + FC group and Ca(OH)(2) + FC group showed the lowest survival rates (p < 0.05). The Ca(OH)(2) + Iodoform + CPC group and Ca(OH)(2) + CPC group showed significantly lower survival rates at concentrations above 6 microL/mL (p < 0.05). The Ca(OH)(2) + Iodoform group and Vitapex group showed the highest survival rates (p < 0.05). We concluded that the use of calcium hydroxide with iodoform as a root filling base material is a better option than other medications.
authors.affiliation: Dental Department, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Tsui-Hsien
authors.initials: TH
authors.lastname: Huang
authors.forename: Shinn-Jyh
authors.initials: SJ
authors.lastname: Ding
authors.forename: Chia-Tze
authors.initials: CT
authors.lastname: Kao
mesh_terms: D002126:Calcium Hydroxide; D002164:Camphor; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002733:Chlorophenols; D004338:Drug Combinations; D005054:Eugenol; D005566:Formocresols; D006801:Humans; D006847:Hydrocarbons, Iodinated; D066298:In Vitro Techniques; D008422:Materials Testing; D012387:Root Canal Filling Materials / Q000633:toxicity; D012828:Silicones; D014094:Tooth, Deciduous / Q000601:surgery*; D015035:Zinc Oxide-Eugenol Cement
chemical_list: D002733:Chlorophenols; D004338:Drug Combinations; D005566:Formocresols; D006847:Hydrocarbons, Iodinated; D012387:Root Canal Filling Materials; D012828:Silicones; C116273:Tifell; D015035:Zinc Oxide-Eugenol Cement; C029584:calcium hydroxide, iodoform, silicone oil drug combination; D005054:Eugenol; D002164:Camphor; C029804:camphorated parachlorophenol; C010473:iodoform; D002126:Calcium Hydroxide