title: [The resistance to glycopeptide antibiotics among strains of Enterococcus sp. isolated in 1998-2005 from patients of one of the university hospitals in Warsaw].
abstract: Infections due to vancomycin resistant enterococci (VRE) constitute a serious therapeutic problem in some cases. Up to date there is only poor knowledge about the frequency of isolation of VRE inthe Polish hospitals. The aim of our investigations was to compare the number and the assortment of VRE isolated during eight years (1998-2005) in the one big clinical hospital in Warsaw. All resistant strains were checked for possessing the vanA or van B ligase genes in the PCR reaction. The identification of the strains was proven by PCR with using of the ddl primers. The significant increase in the number of VRE was observed in the 2005 compared with recent years. All strains isolated in recent years possessed vanA ligase genes. Although among all enterococci E. faecalis made majority (over 70%), among VRE predominated E. faecium.
authors.affiliation: Katedra i Zakład Mikrobiologii Lekarskiej A M w Warszawie.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Młynarczyk
authors.forename: Wanda
authors.initials: W
authors.lastname: Grzybowska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Młynarczyk
authors.forename: Stefan
authors.initials: S
authors.lastname: Tyski
authors.forename: Mirosław
authors.initials: M
authors.lastname: Luczak
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology; D015373:Bacterial Typing Techniques; D019729:Carbon-Oxygen Ligases / Q000502:physiology; D024881:Drug Resistance, Bacterial; D016983:Enterococcus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006020:Glycopeptides / Q000494:pharmacology; D006785:Hospitals, University; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies; D013045:Species Specificity; D020713:Vancomycin Resistance
chemical_list: D001426:Bacterial Proteins; D006020:Glycopeptides; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; D019729:Carbon-Oxygen Ligases