title: Phylogenetic analysis of Nosema antheraeae (Microsporidia) isolated from Chinese oak silkworm, Antheraea pernyi.
abstract: The microsporidian Nosema antheraeae is a pathogen that infects the Chinese oak silkworm, Antheraea pernyi. We sequenced the complete small subunit (SSU) rRNA gene and the internal transcribed spacer (ITS) of N. antheraeae, and compared the SSU rRNA sequences in other microsporidia. The results indicated that Nosema species, including N. antheraeae, formed two distinct clades, consistent with previous observations. Furthermore, N. antheraeae is clustered with N. bombycis with high bootstrap support. The organization of the rRNA gene of N. antheraeae is LSU-ITS1-SSU-ITS2-5S, also following a pattern similar to the Nosema type species, N. bombycis. Thus, N. antheraeae is a Nosema species and has a close relationship to N. bombycis.
authors.affiliation: Institute of Life Sciences, The University of JiangSu, ZhenJiang 212013, China.
authors.forename: Lin-Ling
authors.initials: LL
authors.lastname: Wang
authors.forename: Ke-Ping
authors.initials: KP
authors.lastname: Chen
authors.forename: Ze
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Gui-Tian
authors.initials: GT
authors.lastname: Gao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D012831:Bombyx / Q000382:microbiology*; D002681:China; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005800:Genes, Fungal; D020459:Genes, rRNA; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D016818:Nosema / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics*
chemical_list: D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal