title: Flow cytometric quantification of antigen D sites on red blood cells of partial D and weak D variants in India.
abstract: The D antigenic sites are diminished in weak D and partial D variants. The aim of this study was to estimate D antigen on RBC in weak D and partial D variants in Indian population by using flow cytometric method. Blood samples of 42 cases of partial D, eight cases of weak D and 123 normal Rh phenotypes identified by serological methods were used in the study. An indirect immunofluorescence method was employed using 29 monoclonal anti-D as primary antibody. The D antigenic sites were calculated using control RBC as internal standard. The D antigenic sites in weak D and partial D variants are less than that found in normal Rh phenotypes. In weak D, the D antigenic sites were between 1500 and 7000 D antigens per cell. Among partial D variants, DVI had minimum and DVa had maximum number of D sites. Flow cytometry is a very good tool for demonstrating minor variation in D antigen when serological methods are inconclusive. The D antigenic sites per RBC in partial D variants identified in Indian population are reported for the first time.
authors.affiliation: Institute of Immunohaematology (ICMR), KEM Hospital Campus, Parel, Mumbai, India. swatiskulkarni@hotmail.com
authors.forename: S
authors.initials: S
authors.lastname: Kulkarni
authors.forename: D
authors.initials: D
authors.lastname: Mohanty
authors.forename: S
authors.initials: S
authors.lastname: Gupte
authors.forename: K
authors.initials: K
authors.lastname: Vasantha
authors.forename: S
authors.initials: S
authors.lastname: Joshi
mesh_terms: D000911:Antibodies, Monoclonal; D001665:Binding Sites; D004912:Erythrocytes / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect; D014644:Genetic Variation; D006801:Humans; D007194:India; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System / Q000032:analysis* / Q000235:genetics; D018029:Rho(D) Immune Globulin
chemical_list: D000911:Antibodies, Monoclonal; D007518:Isoantibodies; C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin; C026410:Rho(D) antigen