title: Transcriptional profile of Haemophilus influenzae: effects of iron and heme.
abstract: Haemophilus influenzae requires either heme or a porphyrin and iron source for growth. Microarray studies of H. influenzae strain Rd KW20 identified 162 iron/heme-regulated genes, representing approximately 10% of the genome, with > or =1.5-fold changes in transcription in response to iron/heme availability in vitro. Eighty genes were preferentially expressed under iron/heme restriction; 82 genes were preferentially expressed under iron/heme-replete conditions.
authors.affiliation: Department of Pediatrics, CHO 2308, 940 NE 13th St., Oklahoma City, OK 73104, USA.
authors.forename: Paul W
authors.initials: PW
authors.lastname: Whitby
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Vanwagoner
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Seale
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Morton
authors.forename: Terrence L
authors.initials: TL
authors.lastname: Stull
mesh_terms: D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006193:Haemophilus influenzae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D006418:Heme / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D046228:Microarray Analysis; D018085:Regulon / Q000235:genetics
chemical_list: D003470:Culture Media; D006418:Heme; D007501:Iron