title: [A comparative study on isoenzymes of Sarcocystis spp. from cattle and water buffaloes].
abstract: OBJECTIVE
  To analyze the zymogram of peroxidase (PER) and phosphoglucose isomerase (PGI) of three species of Sarcocystis.
  
  
  METHODS
  The collected parasites were homogenized and fragmented by ultrasonication. After centrifugation, the supernatants were analyzed by isoelectric focusing electrophoresis.
  
  
  RESULTS
  The isolates of S. cruzi from infected water buffalo and cattle all showed identical enzyme profiles, 7 bands of PER at pH 4.44-6.98 and 6 bands of PGI at pH 4.66-6.53; and same with the isolates of S. hirsuta. 5 bands of PER at pH 4.97-7.15 and 4 bands of PGI at pH 4.70-6.51. The zymograms among S. cruzi, S. hirsuta and S. fusiformis were different considerably.
  
  
  CONCLUSIONS
  The data support the hypothesis that both water buffalo and cattle are the natural intermediate hosts of S. cruzi and S. hirsuta at the gene level. S. cruzi, S. hirsuta and S. fusiformis are different species.
authors.affiliation: Bioinformatics College, Chongqing University of Posts and Communications, Chongqing 400065, China.
authors.forename: Yi-long
authors.initials: YL
authors.lastname: Liang
authors.forename: Yong-fang
authors.initials: YF
authors.lastname: Xie
authors.forename: Yang-xian
authors.initials: YX
authors.lastname: Zuo
authors.forename: De-yong
authors.initials: DY
authors.lastname: Tan
authors.forename: Kun-xian
authors.initials: KX
authors.lastname: Shu
authors.forename: Xin-wen
authors.initials: XW
authors.lastname: Chen
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D002417:Cattle / Q000469:parasitology*; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism; D012522:Sarcocystis / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: D007527:Isoenzymes; D015800:Protozoan Proteins; D009195:Peroxidase; D005956:Glucose-6-Phosphate Isomerase