A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp.
Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes. Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular metabolites: DNA, RNA, enzymes, and secondary metabolites. The most suitable method for nucleic acid isolation was grinding under liquid nitrogen, while bead mill homogenization was the superior technique for isolation of active enzymes. A new effective method is proposed for isolation of secondary metabolites with the aid of bead milling of fungal mycelia.
Taskova
Rilka M
RM
Institut für Lebensmittelchemie, Universität Hannover, D-30453 Hannover, Germany.
Zorn
Holger
H
Krings
Ulrich
U
Bouws
Henning
H
Berger
Ralf G
RG
D000363:Agaricales / Q000737:chemistry* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D005656:Fungal Proteins / Q000302:isolation & purification; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry* / Q000378:metabolism; D013053:Spectrophotometry
D005656:Fungal Proteins; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase