Development of a chemiluminescent ELISA for determining chloramphenicol in chicken muscle.
An indirect competitive enzyme-linked immunosorbent assay (ELISA) with chemiluminescent (CL) detection for chloramphenicol (CAP) in chicken muscle was developed. CAP-specific polyclonal antibody was raised in rabbit with a CAP-succinate derivative conjugated with bovine serum albumin. Luminol solution was used as the substrate of horseradish peroxidase. The detection limit was 6 ng/L. The CL-ELISA was 10 times more sensitive compared to the colorimetric-ELISA. When CAP was spiked in chicken muscle at levels of 0.05-5 microg/kg, recoveries ranged from 97 to 118% with coefficients of variation of 6-22%. In an actual residue study, the results obtained by CL-ELISA correlated well with those obtained by gas chromatography with microcell electron capture detector. The residue levels of CAP in treated chicken decreased with time and dropped rapidly after the first 6 h from around 50 to 10 microg/kg. After 3 days, CAP was not detected in chicken muscle. The developed method is therefore suitable for screening of CAP in chicken muscle samples.
Zhang
Suxia
S
Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Zhang
Zhen
Z
Shi
Weimin
W
Eremin
Sergei A
SA
Shen
Jianzhong
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002645:Chickens; D002701:Chloramphenicol / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008163:Luminescent Measurements; D018482:Muscle, Skeletal / Q000737:chemistry*; D011817:Rabbits; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D000906:Antibodies; D002701:Chloramphenicol