PACAP inhibits oxidative stress-induced activation of MAP kinase-dependent apoptotic pathway in cultured cardiomyocytes.
The present article investigated the effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on oxidative stress-induced apoptosis in neonatal rat cardiomyocytes. Our results show that PACAP decreased the ratio of apoptotic cells following H2O2 treatment. PACAP also diminished the activity of apoptosis signal-regulating kinase. These effects of PACAP were counteracted by the PACAP antagonist PACAP6-38. In summary, our results show that PACAP is able to attenuate oxidative stress-induced cardiomyocyte apoptosis and suggest that its cardioprotective effect is mediated through inhibition of the MAP kinase-dependent apoptotic pathway.
Gasz
Balazs
B
Department of Anatomy, University of Pécs, 7624 Pécs, Szigeti u 12. Hungary.
Rácz
Boglarka
B
Röth
Erzsebet
E
Borsiczky
Balazs
B
Tamás
Andrea
A
Boronkai
Arpad
A
Gallyas
Ferenc
F
Tóth
Gabor
G
Reglodi
Dora
D
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D048848:MAP Kinase Kinase Kinase 5 / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D018384:Oxidative Stress; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D048848:MAP Kinase Kinase Kinase 5