Effects of VIP and VIP-DAP on proliferation and lipid peroxidation metabolism in human KB cells.
In the present study, we have utilized the transglutaminase (TGase) enzyme to modify the primary structure of VIP with diaminopropane (DAP) at the level of the Gln16. We have investigated the conformational stability of VIP and VIP-DAP in solution by limited proteolysis experiments. The VIP-DAP appears to be more resistant to the proteolytic attack of trypsin, thus indicating that the derivatization in position 16 is able to stabilize the structure of the peptide. However, we have studied their role in cell cycle modulation and antioxidant activity in the oropharyngeal epidermoid carcinoma KB cells.
Caraglia
Michele
M
Dipartimento di Biochimica e Biofisica, Seconda Università di Napoli, Via Costantinopoli 16, 80138 Napoli, Italy.
Dicitore
Alessandra
A
Giuberti
Gaia
G
Cassese
Diana
D
Lepretti
Marilena
M
Cartenì
Maria
M
Abbruzzese
Alberto
A
Stiuso
Paola
P
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003959:Diamines / Q000494:pharmacology*; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D003959:Diamines; D014660:Vasoactive Intestinal Peptide