Proteomic analysis of pulmonary sclerosing hemangioma.
Sclerosing hemangioma (SH) is a rare benign pulmonary tumor derived from the primitive respiratory epithelium. However, the pathogenesis of SH has not yet been clear. Surfactant protein, thyroid transcription factor-1, epithelial membrane antigen, cytokeratin, and vimentin have been identified in SH by immunohistochemistry and electron microscopy. To identify proteins specifically regulated in SH, 2-D PAGE was performed using SH and paired normal tissues. Ten selected differentially expressed protein spots were identified by PMF, MALDI-TOF-MS, and database searching. Apolipoprotein A-1, antizyme inhibitor, heat shock 27-kDa protein 1, and antioxidant proteins, such as peroxiredoxin II (Prx II) and GST, were identified among the down-regulated proteins in SH. Western blot and immunohistochemistry confirmed reduced expressions of Prx II and GST in SH versus normal lung tissue. This study is the first report on the reduced expressions of Prx II and GST in SH.
Jin
Lian-Jin
LJ
Korea Lung Tissue Bank, Korea University, Seoul, Korea.
Shin
Bong Kyung
BK
Jung
Woon Yong
WY
Lee
Hyun-Juu
HJ
Cho
Su Jin
SJ
Han
Joung-Ho
JH
Ha
Seong-Yeon
SY
Kim
Ae-Ree
AR
Sik Kim
Young
Y
Sun Kim
In
I
Uhm
Chang-Sub
CS
Kim
Han Kyeom
HK
D000328:Adult; D000595:Amino Acid Sequence; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000737:chemistry / Q000378:metabolism; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism; D054464:Peroxiredoxins; D040901:Proteomics; D047868:Pulmonary Sclerosing Hemangioma / Q000378:metabolism* / Q000473:pathology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000737:chemistry
D016328:NF-kappa B; D013438:Sulfhydryl Compounds; D010544:Peroxidases; D054464:Peroxiredoxins