title: Do protein molecules unfold in a simple shear flow?
abstract: Protein molecules typically unfold (denature) when subjected to extremes of heat, cold, pH, solvent composition, or mechanical stress. One might expect that shearing forces induced by a nonuniform fluid flow would also destabilize proteins, as when a protein solution flows rapidly through a narrow channel. However, although the protein literature contains many references to shear denaturation, we find little quantitative evidence for the phenomenon. We have investigated whether a high shear can destabilize a small globular protein to any measurable extent. We study a protein (horse cytochrome c, 104 amino acids) whose fluorescence increases sharply upon unfolding. By forcing the sample through a silica capillary (inner diameter 150-180 microm) at speeds approaching 10 m/s, we subject the protein to shear rates dv(z)/dr as large as approximately 2 x 10(5) s(-1) while illuminating it with an ultraviolet laser. We can readily detect fluorescence changes of <1%, corresponding to shifts of < approximately 0.01 kJ/mol in the stability of the folded state. We find no evidence that even our highest shear rates significantly destabilize the folded protein. A simple model suggests that extraordinary shear rates, approximately 10(7) s(-1), would be required to denature typical small, globular proteins in water.
authors.affiliation: Department of Physics, University of Florida, Gainesville, 32611-8440, USA.
authors.forename: Juan
authors.initials: J
authors.lastname: Jaspe
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Hagen
mesh_terms: D000818:Animals; D003198:Computer Simulation; D045304:Cytochromes c / Q000737:chemistry* / Q000648:ultrastructure*; D006736:Horses; D044085:Microfluidics / Q000379:methods*; D008956:Models, Chemical; D008958:Models, Molecular; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D033081:Shear Strength; D013314:Stress, Mechanical
chemical_list: D045304:Cytochromes c