title: Novel nitrogen-fixing Acetobacter nitrogenifigens sp. nov., isolated from Kombucha tea.
abstract: The four nitrogen-fixing bacteria so far described in the family Acetobacteraceae belong to the genera Gluconacetobacter and Acetobacter. Nitrogen-fixing bacterial strain RG1(T) was isolated from Kombucha tea and, based on the phylogenetic analysis of 16S rRNA gene sequence which is supported by a high bootstrap value, was found to belong to the genus Acetobacter. Strain RG1(T) differed from Acetobacter aceti, the nearest member with a 16S rRNA gene sequence similarity of 98.2 %, and type strains of other Acetobacter species with regard to several characteristics of growth features in culture media, growth in nitrogen-free medium, production of gamma-pyrone from glucose and dihydroxyacetone from glycerol. Strain RG1(T) utilized maltose, glycerol, sorbitol, fructose, galactose, arabinose and ethanol, but not methanol as a carbon source. These results, along with electrophoretic mobility patterns of nine metabolic enzymes, suggest that strain RG1(T) represents a novel nitrogen-fixing species. The ubiquinone present was Q-9 and DNA G+C content was 64.1 mol%. Strain RG1(T) exhibited a low value of 2-24 % DNA-DNA relatedness to the type strains of related acetobacters, which placed it as a separate taxon. On the basis of this data, the name Acetobacter nitrogenifigens sp. nov. is proposed, with the type strain RG1(T) (=MTCC 6912(T)=LMG 23498(T)).
authors.affiliation: Department of Life Science and Biotechnology, Jadavpur University, Kolkata 700032, West Bengal, India.
authors.forename: Debasree
authors.initials: D
authors.lastname: Dutta
authors.affiliation: Department of Life Science and Biotechnology, Jadavpur University, Kolkata 700032, West Bengal, India.
authors.forename: Ratan
authors.initials: R
authors.lastname: Gachhui
mesh_terms: D000091:Acetobacter / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D001482:Base Composition; D002241:Carbohydrates; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004098:Dihydroxyacetone / Q000378:metabolism; D000431:Ethanol; D005516:Food Microbiology; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D011753:Pyrones / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013662:Tea / Q000382:microbiology*; D014451:Ubiquinone / Q000032:analysis
chemical_list: D002241:Carbohydrates; D003470:Culture Media; D004269:DNA, Bacterial; D011753:Pyrones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013662:Tea; D014451:Ubiquinone; D000431:Ethanol; D009584:Nitrogen; D004098:Dihydroxyacetone