Temperature-dependent stress response in oysters, Crassostrea virginica: pollution reduces temperature tolerance in oysters.
Combined effects of temperature and a toxic metal, cadmium (Cd), on energy metabolism were studied in a model marine bivalve, the eastern oyster Crassostrea virginica, acclimated at 20, 24 and 28 degrees C and exposed to 50microgl(-1) of Cd. Both increasing temperature and Cd exposure led to a rise in standard metabolic rates, and combined stressors appeared to override the capability for aerobic energy production resulting in impaired stress tolerance. Oysters exposed to elevated temperature but not Cd showed no significant change in condition, survival rate and lipid peroxidation, whereas those exposed to both Cd and temperature stress suffered high mortality accompanied by low condition index and elevated lipid peroxidation. Furthermore, RNA/DNA ratios indicative of protein synthesis rate, and levels of glutathione, which is involved in metal detoxification, increased in Cd-exposed oysters at 20 degrees C but not at 28 degrees C. Implications of the synergism between elevated temperatures and cadmium stress on energy metabolism of oysters are discussed in the light of the potential effects of climate change on oyster populations in polluted areas.
Lannig
Gisela
G
Biology Department, University of North Carolina at Charlotte, 9201 University City Blvd., Charlotte, NC 28223, USA. glannig@awi-bremerhaven.de
Flores
Jason F
JF
Sokolova
Inna M
IM
D000818:Animals; D002104:Cadmium / Q000032:analysis / Q000633:toxicity*; D049896:Crassostrea / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005978:Glutathione / Q000032:analysis / Q000187:drug effects; D006358:Hot Temperature; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000032:analysis; D009696:Nucleic Acids / Q000032:analysis; D016019:Survival Analysis; D013997:Time Factors; D014877:Water Pollution, Chemical / Q000009:adverse effects*
D009696:Nucleic Acids; D002104:Cadmium; D008315:Malondialdehyde; D005978:Glutathione