title: Identification of genes involved in siderophore transport in Streptomyces coelicolor A3(2).
abstract: The potential iron siderophore transporter genes have been determined from the genome sequence of Streptomyces coelicolor A3(2). One of these gene clusters, cdtABC, was disrupted and characterized to determine its role in the uptake of the siderophores produced by S. coelicolor. Resistance to the siderophore-like antibiotics, salmycin and albomycin, was tested in the parent and cdtABC mutant, showing that the parent, but not the mutant, was sensitive to salmycin, while both were resistant to albomycin. Ferrioxamine competition assays against salmycin suggest that the uptake of salmycin is via a ferrioxamine transport system. However, Fe-55 ferrioxamine B uptake experiments did not reveal any difference between the parent and mutant. This suggests that CdtABC specifically transports salmycin, while ferrioxamine uptake maybe substituted by another transport system.
authors.affiliation: Mikrobiologie/Biotechnologie, Eberhard-Karls-Universität Tübingen, Tübingen, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Bunet
authors.forename: Anita
authors.initials: A
authors.lastname: Brock
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Rexer
authors.forename: Eriko
authors.initials: E
authors.lastname: Takano
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D001692:Biological Transport / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D019295:Computational Biology; D003676:Deferoxamine / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005290:Ferric Compounds / Q000378:metabolism; D005291:Ferrichrome / Q000031:analogs & derivatives / Q000494:pharmacology; D017353:Gene Deletion; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D017262:Siderophores / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005290:Ferric Compounds; D017262:Siderophores; D005291:Ferrichrome; C015657:albomycin; C002577:ferrioxamine B; D003676:Deferoxamine