Methodological approach to minimal residual disease detection by flow cytometry in adult B-lineage acute lymphoblastic leukemia.
A flow cytometric approach to minimal residual disease (MRD) monitoring useful in childhood B-lineage acute lymphoblastic leukemia (ALL) is discussed here in the context of ALL in adults. Of 64 leukemia samples analyzed, 95.3% had at least one abnormal phenotype (57.3% had two or more) as compared to physiologic B-cell precursors in adult bone marrow. The method was sensitive enough to detect one leukemic cell among 10,000 normal cells in 16/19 experiments (84.2%). Blast phenotypes were stable in culture and at relapse, and were useful for MRD monitoring in patients. Marker combinations for childhood ALL are also applicable to adult cases.
Krampera
Mauro
M
Dipartimento di Medicina Clinica e Sperimentale, Sezione di Ematologia, Università di Verona. mauro.krampera@univr.it
Perbellini
Omar
O
Vincenzi
Carlo
C
Zampieri
Francesca
F
Pasini
Annalisa
A
Scupoli
Maria Teresa
MT
Guarini
Anna
A
De Propris
Maria Stefania
MS
Coustan-Smith
Elaine
E
Campana
Dario
D
Foà
Robin
R
Pizzolo
Giovanni
G
D000328:Adult; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000473:pathology; D001854:Bone Marrow Cells / Q000166:cytology / Q000473:pathology; D002051:Burkitt Lymphoma / Q000473:pathology*; D002648:Child; D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D018365:Neoplasm, Residual / Q000473:pathology*; D012016:Reference Values; D012038:Regeneration
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016650:Fluorescein-5-isothiocyanate