title: Determination and pharmacokinetic study of syringin and chlorogenic acid in rat plasma after administration of Aidi lyophilizer.
abstract: A high-performance liquid chromatographic (HPLC) method was developed for the first time to simultaneously quantify syringin and chlorogenic acid in rat plasma using wavelength-transfer technology. The analysis was performed on a Diamonsil C(18) column (200 x 4.6 mm i.d., 5 microm particle size) with isocratic mobile phase consisting of acetonitrile-0.05% phosphoric acid (12:88, v/v). The linear ranges were 0.20-10 and 0.25-30 microg/mL, respectively. The lower limits of quantification were 0.20 and 0.25 microg/mL, respectively. The method was shown to be reproducible and reliable with intraday precision below 8.5 and 6.1%, interday precision below 7.1 and 5.5%, accuracy within +/-7.1 and +/-8.6%, and mean extraction recovery excess of 92.1 and 80.9%, respectively, which were all calculated from the blank plasma sample spiked with syringin and chlorogenic acid at three concentrations of 0.20, 1.0 and 6.0 microg/mL for syringin and 0.25, 2.0 and 20 microg/mL for chlorogenic acid. This method was validated for specificity, accuracy and precision and was successfully applied to the pharmacokinetic study of syringin and chlorogenic acid in rat plasma after intravenous administration of Aidi lyophilizer.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, Liaoning Province, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Sun
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Jia
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Wang
authors.forename: Zhen-Duo
authors.initials: ZD
authors.lastname: Shen
authors.forename: Kai-Shun
authors.initials: KS
authors.lastname: Bi
mesh_terms: D000818:Animals; D002726:Chlorogenic Acid / Q000097:blood / Q000493:pharmacokinetics*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D010666:Phenylpropionates / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D014466:Ultraviolet Rays
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010666:Phenylpropionates; C517300:aidi herbal drug; D002726:Chlorogenic Acid; C028305:syringin