The mechanism by which overexpression of Gts1p induces flocculation in a FLO8-inactive strain of the yeast Saccharomyces cerevisiae.
GTS1 induces flocculation when overexpressed in the Saccharomyces cerevisiae strain W303-1A, which carries a mutant FLO8, the activator of flocculin genes. Herein, we report that the GTS1-induced flocculation was flocculin-dependent in nature and was caused by expression of the major flocculin Flo1p. Gts1p bound to the repressor Sfl1p, and their interaction at the transcriptional level was shown by reporter gene assays using the FLO1 promoter, suggesting that Gts1p induces the expression of FLO1 by inhibiting Sfl1p. Furthermore, the Q-rich domain with the preceding 18 amino acids of Gts1p bound mediators for RNA polymerase II.
Shen
Hua
H
Department of Biochemistry 2, University of Yamanashi, Faculty of Medicine, Shimokato, Tamaho, Nakakoma, Yamanashi, Japan.
Iha
Hidekatsu
H
Yaguchi
So-ichi
S
Tsurugi
Kunio
K
D019677:Artificial Gene Fusion; D015153:Blotting, Western; D005427:Flocculation; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D037241:Mannose-Binding Lectins; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012319:RNA Polymerase II / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
C087578:FLO1 protein, S cerevisiae; C097841:GTS1 protein, S cerevisiae; D037241:Mannose-Binding Lectins; C063404:SFL1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D012319:RNA Polymerase II; D001616:beta-Galactosidase