Hemorphin and hemorphin-like peptides isolated from dog pancreas and sheep brain are able to potentiate bradykinin activity in vivo.
Hemorphins are biologically active peptides, derived from hemoglobin, which presents a number of physiological activities. Proteolytic generation of these peptides is not fully understood; however, among their roles, is to provoke reduction on blood pressure. In this work, this particular biological effect was chosen as the monitor for the selection of mammalian vasoactive peptides. By combining high-performance liquid chromatography and mass spectrometry, including 'de novo' sequencing, several hemorphin-like peptides were identified presenting bradykinin potentiating activity. Moreover, taking LVV-hemorphin-7 as model compound, we evaluated its biological effect on blood pressure of anaesthetized rats. By summarizing all the results, it is possible to present the hemorphins as a family of proteolytically generated peptides that are able to potentiate bradykinin activity in vivo.
Ianzer
Danielle
D
Center for Applied Toxinology, CAT-CEPID, Laboratório Especial de Toxinologia Aplicada, Instituto Butantan, Av. Vital Brasil 1500, Sao Paulo, SP 05503-900, Brazil.
Konno
Katsuhiro
K
Xavier
Carlos Henrique
CH
Stöcklin
Reto
R
Santos
Robson Augusto S
RA
de Camargo
Antônio Carlos Martins
AC
Pimenta
Daniel Carvalho
DC
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D001794:Blood Pressure / Q000187:drug effects; D001920:Bradykinin / Q000819:agonists / Q000494:pharmacology / Q000502:physiology*; D001923:Brain Chemistry; D004285:Dogs; D004357:Drug Synergism; D005260:Female; D006168:Guinea Pigs; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D010179:Pancreas / Q000737:chemistry*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012756:Sheep; D015854:Up-Regulation / Q000187:drug effects
D006454:Hemoglobins; D010446:Peptide Fragments; D010455:Peptides; C085122:hemorphin 7; D001920:Bradykinin