Nourseothricin acetyltransferease: a positive selectable marker for Toxoplasma gondii.
Molecular analysis of parasite genomes will require new molecular genetic tools. The nat1 gene of Streptomyces noursei encodes nourseothricin acetyltransferase, conferring resistance to the aminoglycoside antibiotic nourseothricin. Electroporation of nat1 cassettes into RH or Prugniaud strains of Toxoplasma gondii allows for selection of stable nourseothricin-resistant clones.
Van Tam
T
T
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 53706, USA.
Rooney
Peggy J
PJ
Knoll
Laura J
LJ
D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015139:Blotting, Southern; D004351:Drug Resistance / Q000235:genetics; D018274:Electroporation; D005819:Genetic Markers; D006801:Humans; D008297:Male; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics; D013309:Streptothricins / Q000494:pharmacology*; D014122:Toxoplasma / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
D005819:Genetic Markers; D013309:Streptothricins; D000123:Acetyltransferases; C050473:nourseothricin acetyltransferase