title: Z39Ig is expressed on macrophages and may mediate inflammatory reactions in arthritis and atherosclerosis.
abstract: Z39Ig is a transmembrane protein containing two Ig homology domains with unknown functions. Immunohistochemical analyses of human carotid atherosclerotic plaques detected Z39Ig staining in areas rich in foamy macrophages. Z39Ig staining was also observed in macrophages in the lining layers and sublining areas of rheumatoid arthritis synovium. Z39Ig staining in the osteoarthritis synovium was restricted to macrophages in the lining layers. To identify the role(s) of Z39Ig in the function of macrophages, we used human monocytic cell lines TF-1A (Z39Ig-negative) and THP-1 (Z39Ig-positive). The expression of Z39Ig was induced in TF-1A cells ,when they were differentiated into macrophages by treatment with PMA. The stimulation of PMA-treated TF-1A or THP-1 cells with immobilized anti-Z39Ig mAb induced the secretion of IL-8 and matrix metalloproteinase (MMP)-9, which was dependent on NF-kappaB activation. These data indicate that the macrophage Z39Ig is involved in the pathogenesis of inflammatory diseases through chemokine induction, which will promote the migration of inflammatory cells into the lesion area, and MMP-9 induction, which will contribute to cartilage destruction or extracellular matrix degradation.
authors.affiliation: Department of Genetic Engineering, Kyungpook National University, Taegu 702-701, Korea.
authors.forename: Min-Young
authors.initials: MY
authors.lastname: Lee
authors.forename: Won-Jung
authors.initials: WJ
authors.lastname: Kim
authors.forename: Yoon-Joong
authors.initials: YJ
authors.lastname: Kang
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Jung
authors.forename: Young-Mo
authors.initials: YM
authors.lastname: Kang
authors.forename: Kyoungho
authors.initials: K
authors.lastname: Suk
authors.forename: Jeong-Euy
authors.initials: JE
authors.lastname: Park
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Choi
authors.forename: Beom-Kyu
authors.initials: BK
authors.lastname: Choi
authors.forename: Byoung S
authors.initials: BS
authors.lastname: Kwon
authors.forename: Won-Ha
authors.initials: WH
authors.lastname: Lee
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D000937:Antigen-Antibody Reactions; D001168:Arthritis / Q000276:immunology*; D050197:Atherosclerosis / Q000276:immunology*; D002460:Cell Line; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D016209:Interleukin-8 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D016328:NF-kappa B / Q000276:immunology; D011951:Receptors, Complement / Q000096:biosynthesis* / Q000187:drug effects / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D016209:Interleukin-8; D016328:NF-kappa B; D011951:Receptors, Complement; C414427:VSIG4 protein, human; D020780:Matrix Metalloproteinase 9; D013755:Tetradecanoylphorbol Acetate