Human IRGM induces autophagy to eliminate intracellular mycobacteria.
Immunity-related p47 guanosine triphosphatases (IRG) play a role in defense against intracellular pathogens. We found that the murine Irgm1 (LRG-47) guanosine triphosphatase induced autophagy and generated large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. We also identified a function for a human IRG protein in the control of intracellular pathogens and report that the human Irgm1 ortholog, IRGM, plays a role in autophagy and in the reduction of intracellular bacillary load.
Singh
Sudha B
SB
Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
Davis
Alexander S
AS
Taylor
Gregory A
GA
Deretic
Vojo
V
D000818:Animals; D001343:Autophagy; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D008247:Lysosomes / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D050296:Microbial Viability; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009163:Mycobacterium bovis / Q000276:immunology* / Q000502:physiology; D010588:Phagosomes / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure*; D034741:RNA, Small Interfering; D014162:Transfection; D014617:Vacuoles / Q000378:metabolism / Q000648:ultrastructure
C096000:Ifi1 protein, mouse; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering; D007371:Interferon-gamma; D019204:GTP-Binding Proteins; C513539:IRGM protein, human