title: Diversified serum IgG response involving non-myelin CNS proteins during experimental autoimmune encephalomyelitis.
abstract: We sequentially analyzed the serum IgG response against normal mouse brain during experimental autoimmune encephalomyelitis in SJL/J mice injected with CFA, Bordetella pertussis toxin (BPT) and proteolipid protein 139-151 peptide, compared with mice that received CFA and BPT or were uninjected. Dynamic changes were observed from day 0 to day 28 in the 3 groups. Six highly discriminant antigenic bands (kappa=0.974) were identified. Three non-myelin proteins were characterized (mitochondrial aconitase hydratase 2, phosphoglycerate mutase 1, brain specific pyruvate deshydrogenase). The IgG response against two of them was less frequent in EAE whereas it was associated with multiple sclerosis in our previous work.
authors.affiliation: Laboratoire d'Immunologie EA2686, Faculté de Médecine, 1, Place de Verdun, Lille Cedex, France. H-ZEPHIR@chru-lille.fr
authors.forename: Helene
authors.initials: H
authors.lastname: Zephir
authors.forename: Lionel
authors.initials: L
authors.lastname: Almeras
authors.forename: Mohamed
authors.initials: M
authors.lastname: El Behi
authors.forename: Patricia
authors.initials: P
authors.lastname: Dussart
authors.forename: Jerome
authors.initials: J
authors.lastname: de Seze
authors.forename: Jerome
authors.initials: J
authors.lastname: Steibel
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Trifilieff
authors.forename: Sylvain
authors.initials: S
authors.lastname: Dubucquoi
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Dessaint
authors.forename: Patrick
authors.initials: P
authors.lastname: Vermersch
authors.forename: Lionel
authors.initials: L
authors.lastname: Prin
authors.forename: Didier
authors.initials: D
authors.lastname: Lefranc
mesh_terms: D000154:Aconitate Hydratase / Q000276:immunology; D000818:Animals; D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000276:immunology*; D015153:Blotting, Western; D001921:Brain / Q000276:immunology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000097:blood* / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood*; D051379:Mice; D018991:Myelin Proteolipid Protein / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D010736:Phosphoglycerate Mutase / Q000276:immunology; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D007074:Immunoglobulin G; D018991:Myelin Proteolipid Protein; D010446:Peptide Fragments; D011768:Pyruvate Dehydrogenase Complex; C435023:myelin proteolipid protein (139-151); D000154:Aconitate Hydratase; D010736:Phosphoglycerate Mutase