title: Virus-like particles derived from major capsid protein VP1 of different polyomaviruses differ in their ability to induce maturation in human dendritic cells.
abstract: As polyomavirus major capsid protein VP1-derived virus-like particles (VLPs) have been demonstrated to be highly immunogenic, we studied their interaction with human dendritic cells (hDCs). Exposure of hDCs to VLPs originating from murine (MPyV) or hamster polyomavirus (HaPyV) induced hDC maturation. In contrast, exposure of hDCs to VLPs derived from human polyomaviruses (BK and JC) and simian virus 40 (SV40) only marginally induced DC maturation. The hDCs stimulated by HaPyV- or MPyV-derived VLPs readily produced interleukin-12 and stimulated CD8-positive T-cell responses in vitro. The highest frequencies of activated T cells were again observed after pulsing with HaPyV- and MPyV-derived VLPs. Monocyte-derived hDCs both bound and internalized the various tested polyomavirus VP1-derived VLPs with different levels of efficiency, partially explaining their individual maturation potentials. In conclusion, our data suggest a high variability in uptake of polyomavirus-derived VLPs and potency to induce hDC maturation.
authors.affiliation: Institute of Biotechnology, V Graiciuno 8, LT-02241 Vilnius, Lithuania.
authors.forename: Alma
authors.initials: A
authors.lastname: Gedvilaite
authors.forename: David C
authors.initials: DC
authors.lastname: Dorn
authors.forename: Kestutis
authors.initials: K
authors.lastname: Sasnauskas
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pecher
authors.forename: Aiste
authors.initials: A
authors.lastname: Bulavaite
authors.forename: Robert
authors.initials: R
authors.lastname: Lawatscheck
authors.forename: Juozas
authors.initials: J
authors.lastname: Staniulis
authors.forename: Tina
authors.initials: T
authors.lastname: Dalianis
authors.forename: Torbjörn
authors.initials: T
authors.lastname: Ramqvist
authors.forename: Günther
authors.initials: G
authors.lastname: Schönrich
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Raftery
authors.forename: Rainer
authors.initials: R
authors.lastname: Ulrich
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D006801:Humans; D011120:Polyomavirus / Q000235:genetics / Q000502:physiology*; D014771:Virion / Q000502:physiology*
chemical_list: D036022:Capsid Proteins; C062481:VP1 protein, polyomavirus