title: Rhodococcus imtechensis sp. nov., a nitrophenol-degrading actinomycete.
abstract: A Gram-positive actinobacterium, strain RKJ300(T), capable of utilizing p-nitrophenol and 2,4-dinitrophenol, was isolated from a pesticide-contaminated site in India. The morphological and chemotaxonomic properties of the isolate were typical of members of the genus Rhodococcus. The DNA G+C content was 72 mol%. Strain RKJ300(T) exhibited the highest level of sequence similarity with Rhodococcus wratislaviensis NCIMB 13082(T) (99.3 %), followed by Rhodococcus opacus DSM 43205(T) (98.8 %), Rhodococcus percolatus MBS1(T) (98.6 %) and Rhodococcus koreensis DNP505(T) (98.1 %). The low levels of DNA-DNA relatedness (49-58 %) with the above micro-organisms, and the differences in the biochemical and physiological properties, suggest that strain RKJ300(T) should be classified within a novel species of the genus Rhodococcus, for which the name Rhodococcus imtechensis sp. nov. is proposed. The type strain is RKJ300(T) (=MTCC 7085(T)=JCM 13270(T)).
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector-39A, Chandigarh -160 036, India.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector-39A, Chandigarh -160 036, India.
authors.forename: Debarati
authors.initials: D
authors.lastname: Paul
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector-39A, Chandigarh -160 036, India.
authors.forename: Dhan
authors.initials: D
authors.lastname: Prakash
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector-39A, Chandigarh -160 036, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Mayilraj
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector-39A, Chandigarh -160 036, India.
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Jain
mesh_terms: D001482:Base Composition; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007194:India; D008969:Molecular Sequence Data; D009596:Nitrophenols / Q000378:metabolism*; D010575:Pesticides; D010802:Phylogeny; D012240:Rhodococcus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D012989:Soil Pollutants; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D009596:Nitrophenols; D010575:Pesticides; D012989:Soil Pollutants