[Study on synthesis and purification of calcium 5-formyltetrahydrofolate].
OBJECTIVE
To establish a new approach to synthesis and purification of calcium 5-formytetrahydrofolate.


METHODS
Target compound was synthesized by the use of folic acid as starting material via reduction, formylation, hydrolysis and salt formation


RESULTS
The structure of calcium 5-formytetrahydrofolate was confirmed by UV, 1H-NMR and elemental analysis and the overall yield was 54%-59%, 14%-17% higher than the reported yield.


CONCLUSIONS
A convenient synthetic route was developed and it would be suitable for industrial production.
Wang
Biao
B
Department of Chemical Biology, School of Pharmaceutical Science, State Key laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100083, China.
Zhang
Zhi-li
ZL
Guo
Ying
Y
Wu
Xiao-hua
XH
Liu
Jun-yi
JY
D002118:Calcium / Q000737:chemistry*; D002955:Leucovorin / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet
D002955:Leucovorin; D002118:Calcium