Effect of methionine loading on pulse wave analysis in elderly volunteers.
OBJECTIVE
To test the hypothesis that an acute increase in plasma homocysteine produced by methionine is associated with an acute increase in pulse wave velocity.


METHODS
A double blind, cross over, placebo controlled design was used and pulse wave velocity, plasma homocysteine, total cholesterol: high density lipoprotein ratio, plasma triglyceride, oxidised low density lipoprotein cholesterol concentrations, apolipoproteins A1 and B, and C reactive protein were measured between 12.5 and 20 hours after methionine loading or placebo.


RESULTS
Between 12.5 and 20 hours after exposure to a methionine loading test, arterial pulse wave velocity showed no significant difference compared with placebo. At 12 hours after exposure to the methionine loading test, in the presence of a controlled diet, triglyceride concentration significantly increased by 32.6% (p<0.02), cholesterol: high density lipoprotein ratio increased significantly by 22.5% (p<0.05) compared with placebo. Simultaneously, systolic blood pressure increased significantly by 4.9% (p<0.02).


CONCLUSIONS
In elderly volunteers, acute hyperhomocysteinaemia induced by methionine loading resulted in no overall significant delayed reduction in peripheral arterial distensibility. A significant deterioration in the lipid profile and increased blood pressure was seen during acute hyperhomocysteinaemia.
Hart
S R
SR
Division of Clinical Neuroscience, University of Edinburgh, Western General Hospital, Edinburgh, UK. sh@skull.dcn.ed.ac.uk
Mangoni
A A
AA
Swift
C G
CG
Jackson
S H D
SH
D000368:Aged; D001783:Blood Flow Velocity / Q000187:drug effects; D002097:C-Reactive Protein / Q000378:metabolism; D003187:Compliance; D018592:Cross-Over Studies; D004311:Double-Blind Method; D004347:Drug Interactions; D006710:Homocysteine / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000097:blood; D008715:Methionine / Q000494:pharmacology*; D008875:Middle Aged; D011673:Pulsatile Flow / Q000187:drug effects / Q000502:physiology
D008055:Lipids; D006710:Homocysteine; D002097:C-Reactive Protein; D008715:Methionine