title: Phenylobacterium zucineum sp. nov., a facultative intracellular bacterium isolated from a human erythroleukemia cell line K562.
abstract: A bacterial strain HLK1(T) was isolated from the human erythroleukemia cell line K562. This bacterium is a Gram-negative rod, motile with a polar flagellum. It is strictly aerobic, nonfermentative, and oxidase and catalase positive. Its optimal growth occurs at 37 degrees C at pH between 6.5 and 7.5. Phylogenetically, although it shares 98% similarity with the 16S rRNA of Phenylobacterium lituiforme, the DNA-DNA hybridization value between the two species is only 43%. HLK1(T) has a DNA G+C content of 71.2+/-0.2 mol%. It is a facultative intracellular organism and may have pathogenic relevance with humans and mammals. On the basis of the phylogenetic and phenotypic characterization, strain HLK1(T) is proposed to be classified in the genus Phenylobacterium, as P. zucineum sp. nov. The type strain is HLK1(T) (=CGMCC 1.3786(T), DSM=18354).
authors.affiliation: Cancer Institute, The Second Affiliated Hospital, Zhejiang University Medical School, 88 Jiefang Road, Hangzhou 310009, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Weidong
authors.initials: W
authors.lastname: Han
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.forename: Qiangrong
authors.initials: Q
authors.lastname: Pan
authors.forename: Xun
authors.initials: X
authors.lastname: Hu
mesh_terms: D042063:Caulobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase