title: Complete nucleotide sequence, genomic organization and phylogenetic analysis of Citrus leprosis virus cytoplasmic type.
abstract: The complete nucleotide sequence of the genomic RNA 1 (8745 nt) and RNA 2 (4986 nt) of Citrus leprosis virus cytoplasmic type (CiLV-C) was determined using cloned cDNA. RNA 1 contains two open reading frames (ORFs), which correspond to 286 and 29 kDa proteins. The 286 kDa protein is a polyprotein putatively involved in virus replication, which contains four conserved domains: methyltransferase, protease, helicase and polymerase. RNA 2 contains four ORFs corresponding to 15, 61, 32 and 24 kDa proteins, respectively. The 32 kDa protein is apparently involved in cell-to-cell movement of the virus, but none of the other putative proteins exhibit any conserved domain. The 5' regions of the two genomic RNAs contain a 'cap' structure and poly(A) tails were identified in the 3'-terminals. Sequence analyses and searches for structural and non-structural protein similarities revealed conserved domains with members of the genera Furovirus, Bromovirus, Tobravirus and Tobamovirus, although phylogenetic analyses strongly suggest that CiLV-C is a member of a distinct, novel virus genus and family, and definitely demonstrate that it does not belong to the family Rhabdoviridae, as previously proposed. Based on these results it was proposed that Citrus leprosis virus be considered as the type member of a new genus of viruses, Cilevirus.
authors.affiliation: UNESP, Universidade Estadual Paulista, Instituto de Biociências, Rod. D. Sartori 21-500, Distrito Rubião Jr 18618-000, Botucatu/SP, Brazil.|Centro APTA Citros Sylvio Moreira, IAC, Rod. Anhanguera, Km 158, CP 4, 13490-970 Cordeirópolis/SP, Brazil.
authors.forename: E C
authors.initials: EC
authors.lastname: Locali-Fabris
authors.affiliation: EMBRAPA Milho e Sorgo, Rod. MG 424, Km 65, CP 151, 35701-970 Sete Lagoas/MG, Brazil.|Centro APTA Citros Sylvio Moreira, IAC, Rod. Anhanguera, Km 158, CP 4, 13490-970 Cordeirópolis/SP, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Freitas-Astúa
authors.affiliation: Centro APTA Citros Sylvio Moreira, IAC, Rod. Anhanguera, Km 158, CP 4, 13490-970 Cordeirópolis/SP, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Souza
authors.affiliation: Centro de Recursos Genéticos Vegetais/IAC, CP 28, 13001-970 Campinas/SP, Brazil.|Centro APTA Citros Sylvio Moreira, IAC, Rod. Anhanguera, Km 158, CP 4, 13490-970 Cordeirópolis/SP, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Takita
authors.affiliation: Centro APTA Citros Sylvio Moreira, IAC, Rod. Anhanguera, Km 158, CP 4, 13490-970 Cordeirópolis/SP, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Astúa-Monge
authors.affiliation: Centro APTA Citros Sylvio Moreira, IAC, Rod. Anhanguera, Km 158, CP 4, 13490-970 Cordeirópolis/SP, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Antonioli-Luizon
authors.affiliation: Centro APTA Citros Sylvio Moreira, IAC, Rod. Anhanguera, Km 158, CP 4, 13490-970 Cordeirópolis/SP, Brazil.
authors.forename: V
authors.initials: V
authors.lastname: Rodrigues
authors.affiliation: Centro APTA Citros Sylvio Moreira, IAC, Rod. Anhanguera, Km 158, CP 4, 13490-970 Cordeirópolis/SP, Brazil.
authors.forename: M L P N
authors.initials: MLPN
authors.lastname: Targon
authors.affiliation: Centro APTA Citros Sylvio Moreira, IAC, Rod. Anhanguera, Km 158, CP 4, 13490-970 Cordeirópolis/SP, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Machado
mesh_terms: D001483:Base Sequence; D002957:Citrus / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics*
chemical_list: D012367:RNA, Viral