Bacterial extracellular DNA forming a defined network-like structure.
It is generally assumed that nucleic acids are localized inside of living cells and that their primary function is the storage of information. In contrast, extracellular DNA is mainly considered as a remnant of lysed cells. Here, we report the formation of extracellular bacterial DNA as a spatial structure. An aquatic bacterium, strain F8, was isolated, which produced a stable filamentous network of extracellular DNA. Different staining and enzymatic techniques confirmed that it was DNA. We were able to amplify the 16S rRNA gene from the extracellular DNA. Restriction endonuclease cleavage and randomly amplified polymorphic DNA analysis of extracellular and genomic DNAs revealed major similarities, but also some differences in both sequences. Our data demonstrate a new function and relevance for extracellular DNA.
Böckelmann
Uta
U
Department of Environmental Microbiology, University of Technology Berlin, Berlin, Germany. uta.boeckelmann@tu-berlin.de
Janke
Andrea
A
Kuhn
Ramona
R
Neu
Thomas R
TR
Wecke
Jörg
J
Lawrence
John R
JR
Szewzyk
Ulrich
U
D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013194:Staining and Labeling; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S