A Mutation in the luxS gene influences Listeria monocytogenes biofilm formation.
Using a Vibrio harveyi reporter strain, we demonstrated that Listeria monocytogenes secretes a functional autoinducer 2 (AI-2)-like signal. A luxS-deficient mutant produced a denser biofilm and attached to a glass surface 19-fold better than the parent strain. Exogenous AI-2 failed to restore the wild-type phenotype to the mutant. It seems that an intact luxS gene is associated with repression of components required for attachment and biofilm formation.
Sela
Shlomo
S
Microbial Food-Safety Research Unit, Department of Food Sciences, Institute for Technology and Storage of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, Beth-Dagan 50250, Israel. shlomos@volcani.agri.gov.il
Frank
Shmulik
S
Belausov
Eddy
E
Pinto
Riky
R
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D013437:Carbon-Sulfur Lyases / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D007783:Lactones / Q000378:metabolism; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009154:Mutation; D016415:Sequence Alignment
D001426:Bacterial Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria