Formation of glucoside conjugate of acetaminophen by fungi separated from soil.
The phase II metabolite of acetaminophen in filamentous fungi and actinomycetes separated from soil was investigated. Fifty-four filamentous fungi and twenty-seven actinomycetes were screened to transform acetaminophen. The metabolites of acetaminophen were assayed using liquid chromatography-tandem mass spectrometry. The only metabolite was subject to enzymatic hydrolysis to confirm its structure. Acetaminophen was converted into glucoside conjugate, by filamentous fungi JX1-60, LN17-2, LN20-1 and the yield of the conjugate was 60.01%, 44.27%, 100%, respectively, and no phase I metabolites were detected. Glucoside conjugation of acetaminophen in filamentous fungi differs from the phase II metabolism of glucuronidation in humans. The fungus LN20-1 could be a suitable model to synthesize glucoside conjugate of acetaminophen.
Huang
H H
HH
Shanghai Drug-Metab Biotech Co., Ltd, People's Republic of China.
Lin
L H
LH
Zhang
P
P
Qi
X L
XL
Zhong
D F
DF
D000082:Acetaminophen / Q000737:chemistry / Q000378:metabolism*; D039903:Actinobacteria / Q000378:metabolism; D018712:Analgesics, Non-Narcotic; D002681:China; D002853:Chromatography, Liquid; D005658:Fungi / Q000378:metabolism*; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D050217:Metabolic Detoxication, Phase II; D015394:Molecular Structure; D012988:Soil Microbiology; D021241:Spectrometry, Mass, Electrospray Ionization
D018712:Analgesics, Non-Narcotic; D005960:Glucosides; D000082:Acetaminophen