Immobilized iminobiotin on magnetic poly (vinyl alcohol) microspheres for single-step purification of streptavidin.
Magnetic poly (vinyl alcohol) microspheres (MPVAMS) immobilized with iminobiotin as the ligand were prepared for the purification of streptavidin. Parameters of magnetic separation, including spacers, NHS-iminobiotin added, elution behavior, and sample treatment, were optimized to improve the purification efficiency to achieve the maximum recovery of the protein. Streptavidin was successfully purified 38-fold from culture supernatant in a single-step by iminobiotin-MPVAMS with an overall recovery of 90.15% and purity of 95.08%. Hence, this study effectively illustrated the favorable application of magnetic microcarriers for the purification of streptavidin.
Cao
Yu
Y
Department of Microbiology, College of Life Sciences, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin, PR China.
Bai
Gang
G
Zhang
Lei
L
Bai
Fang
F
Yang
Wenbo
W
D019149:Bioreactors / Q000382:microbiology; D002846:Chromatography, Affinity / Q000379:methods*; D007218:Industrial Microbiology / Q000379:methods; D008280:Magnetics / Q000295:instrumentation; D008863:Microspheres; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D019809:Streptavidin / Q000302:isolation & purification*; D013302:Streptomyces / Q000378:metabolism
D011142:Polyvinyl Alcohol; D019809:Streptavidin