Genetic diversity of Gram-negative, proteolytic, psychrotrophic bacteria isolated from refrigerated raw milk.
The random amplified polymorphic DNA (RAPD) fingerprinting technique was used to assess the genetic diversity of 70 isolates of Gram-negative proteolytic psychrotrophic bacteria that were isolated from refrigerated raw milk. Three oligonucleotides, which generated 87 fragments of polymorphic DNA, were used in the amplification reactions. The genetic distance values calculated using Jaccard's coefficient showed there was high genetic variability among the isolates. Cluster analysis procedures suggested that the genetic variability among isolates belonging to the same species was as high as the variability among different species. Clustering by the UPGMA hierarchical method and data graph dispersion indicated a tendency of the isolates to group according to whether they did or did not ferment glucose.
Martins
Maurilio L
ML
Department of Microbiology, Federal University of Viçosa, Viçosa, MG 36570.000, Brazil.
Pinto
Claudia L O
CL
Rocha
Rodrigo B
RB
de Araújo
Elza F
EF
Vanetti
Maria C D
MC
D000818:Animals; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005784:Gene Amplification; D014644:Genetic Variation; D006090:Gram-Negative Bacteria / Q000235:genetics*; D006801:Humans; D008892:Milk / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012034:Refrigeration; D013045:Species Specificity
D004269:DNA, Bacterial