title: Architecture of the vimentin cytoskeleton is modified by perturbation of the GTPase ARF1.
abstract: Intermediate filaments are required for proper membrane protein trafficking. However, it remains unclear whether perturbations in vesicular membrane transport result in changes in the architecture of the vimentin cytoskeleton. We find that treatment of cells with Brefeldin A, an inhibitor of specific stages of membrane transport, causes changes in the organization of vimentin filaments. These changes arise from movement of pre-existing filaments. Brefeldin A treatment also leads to alterations in the microtubule cytoskeleton. However, this effect is not observed in cells lacking intermediate filaments, indicating that microtubule bundling is downstream of perturbations in the vimentin cytoskeleton. Brefeldin A-induced changes in vimentin architecture are probably mediated through its effects on ADP-ribosylation factor 1 (ARF1). Expression of a dominant-negative mutant of ARF1 induces BFA-like modifications in vimentin morphology. The BFA-dependent changes in vimentin architecture occurred concurrently with the release of the ARF1-regulated adaptor complexes AP-3 and AP-1 from membranes and adaptor redistribution to vimentin networks. These observations indicate that perturbation of the vesicular membrane transport machinery lead to reciprocal changes in the architecture of vimentin networks.
authors.affiliation: Graduate Program in Biochemistry, Cell, and Developmental Biology, Emory University, Atlanta, GA 30322, USA.
authors.forename: Melanie L
authors.initials: ML
authors.lastname: Styers
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Kowalczyk
authors.forename: Victor
authors.initials: V
authors.lastname: Faundez
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000235:genetics / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D020126:Brefeldin A / Q000494:pharmacology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D048430:Cell Shape; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D014746:Vimentin / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D011500:Protein Synthesis Inhibitors; D014746:Vimentin; D020126:Brefeldin A; D000244:Adenosine Diphosphate; D020823:ADP-Ribosylation Factor 1