title: Core/Shell nanoparticles with lecithin lipid cores for protein delivery.
abstract: Core/shell nanoparticles with lipid core, were prepared and characterized as a sustained delivery system for protein. The lipid core is composed of protein-loaded lecithin and the polymeric shell is composed of Pluronics (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer, F-127). Based on the preparation method in the previous report by us, the freeze-drying of protein-loaded lecithin was performed in the F-127 aqueous solution containing trehalose used as a cryoprotectant to form stabilized core/shell nanoparticles. Cryo-TEM (transmittance electron microscopy) and a particle size analyzer were used to observe the formation of stabilized core/shell nanoparticles. For the application of core/shell nanoparticles as a protein drug carrier, lysozyme and vascular endothelial growth factor (VEGF) were loaded into the core/shell nanoparticles by electrostatic interaction, and the drug release pattern was observed by manipulating the polymeric shell.
authors.affiliation: Department of Polymer Science and Engineering, Hannam University, 133 Ojeong Dong, Daedeog Ku, Taejeon, Korea.
authors.forename: Keun Sang
authors.initials: KS
authors.lastname: Oh
authors.forename: Sung Kyun
authors.initials: SK
authors.lastname: Han
authors.forename: Hyun Suk
authors.initials: HS
authors.lastname: Lee
authors.forename: Hyoung Mo
authors.initials: HM
authors.lastname: Koo
authors.forename: Ree Sun
authors.initials: RS
authors.lastname: Kim
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Lee
authors.forename: Sung Sik
authors.initials: SS
authors.lastname: Han
authors.forename: Sun Hang
authors.initials: SH
authors.lastname: Cho
authors.forename: Soon Hong
authors.initials: SH
authors.lastname: Yuk
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D009113:Muramidase / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010713:Phosphatidylcholines / Q000737:chemistry*; D020442:Poloxamer / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D010713:Phosphatidylcholines; D042461:Vascular Endothelial Growth Factor A; D020442:Poloxamer; D009113:Muramidase