NQO1 and NQO2 regulation of humoral immunity and autoimmunity.
NAD(P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase 2 (NQO2) are cytosolic enzymes that catalyze metabolic reduction of quinones and derivatives. NQO1-null and NQO2-null mice were generated that showed decreased lymphocytes in peripheral blood, myeloid hyperplasia, and increased sensitivity to skin carcinogenesis. In this report, we investigated the in vivo role of NQO1 and NQO2 in immune response and autoimmunity. Both NQO1-null and NQO2-null mice showed decreased B-cells in blood, lower germinal center response, altered B cell homing, and impaired primary and secondary immune responses. NQO1-null and NQO2-null mice also showed susceptibility to autoimmune disease as revealed by decreased apoptosis in thymocytes and pre-disposition to collagen-induced arthritis. Further experiments showed accumulation of NADH and NRH, cofactors for NQO1 and NQO2, indicating altered intracellular redox status. The studies also demonstrated decreased expression and lack of activation of immune-related factor NF-kappaB. Microarray analysis showed altered chemokines and chemokine receptors. These results suggest that the loss of NQO1 and NQO2 leads to altered intracellular redox status, decreased expression and activation of NF-kappaB, and altered chemokines. The results led to the conclusion that NQO1 and NQO2 are endogenous factors in the regulation of immune response and autoimmunity.
Iskander
Karim
K
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA.
Li
Jessica
J
Han
Shuhua
S
Zheng
Biao
B
Jaiswal
Anil K
AK
D000818:Animals; D000917:Antibody Formation; D017209:Apoptosis; D001168:Arthritis / Q000378:metabolism; D015551:Autoimmunity; D001854:Bone Marrow Cells / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone); D009252:NADPH Dehydrogenase / Q000378:metabolism / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism; D010084:Oxidation-Reduction; D011808:Quinone Reductases / Q000378:metabolism / Q000502:physiology*
D016328:NF-kappa B; D016660:NAD(P)H Dehydrogenase (Quinone); C491732:Nqo1 protein, mouse; C419642:NRH - quinone oxidoreductase2; D011808:Quinone Reductases; D009252:NADPH Dehydrogenase