title: Nitric oxide-evoked cGMP production in Purkinje cells in rat cerebellum: an immunocytochemical and pharmacological study.
abstract: The cerebellar cells that account for glutamate-dependent cyclic GMP (cGMP) production, involving activation of the ionotropic glutamate receptors/nitric oxide synthase/soluble guanylyl cyclase pathway, are not fully established. In the present paper we have searched for the localisation of the cGMP response to the nitric oxide (NO) donor S-nitroso-penicillamine (SNAP 1muM), expected to generate local NO concentrations in the low nanomolar physiological range and evoking a cGMP response dependent on glutamate release and on the consequent activation of ionotropic glutamate NMDA/non-NMDA receptors, in cerebellar slices from adult rat. We have found that low concentration of exogenous NO evoked cGMP accumulation in Purkinje cells in an ionotropic glutamate receptor-dependent and tetrodotoxin-sensitive manner. Such immunocytochemical localisation appears consistent with functional evidence for physiologically relevant glutamate-dependent cGMP production in Purkinje cells in rat cerebellar cortex.
authors.affiliation: Dipartimento di Medicina Sperimentale, Sezione di Farmacologia e Tossicologia, Università di Genova, 16148 Genova, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Marcoli
authors.forename: Guido
authors.initials: G
authors.lastname: Maura
authors.forename: Chiara
authors.initials: C
authors.lastname: Cervetto
authors.forename: Caterina
authors.initials: C
authors.lastname: Giacomini
authors.forename: Diana
authors.initials: D
authors.lastname: Oliveri
authors.forename: Simona
authors.initials: S
authors.lastname: Candiani
authors.forename: Mario
authors.initials: M
authors.lastname: Pestarino
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D064026:Calbindins; D002525:Cerebellar Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006152:Cyclic GMP / Q000096:biosynthesis*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D009924:Organ Culture Techniques; D011689:Purkinje Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D017470:Receptors, Glutamate / Q000187:drug effects / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D026941:Sodium Channel Blockers / Q000494:pharmacology; D000071756:Soluble Guanylyl Cyclase; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology
chemical_list: D015415:Biomarkers; D064026:Calbindins; D018691:Excitatory Amino Acid Antagonists; D005904:Glial Fibrillary Acidic Protein; D020030:Nitric Oxide Donors; D018160:Receptors, Cytoplasmic and Nuclear; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D064030:S100 Calcium Binding Protein G; D026941:Sodium Channel Blockers; D009569:Nitric Oxide; D018698:Glutamic Acid; D052248:Nitric Oxide Synthase Type I; D006162:Guanylate Cyclase; D000071756:Soluble Guanylyl Cyclase; D006152:Cyclic GMP