Evaluation of pteridine metabolism in battery workers chronically exposed to lead.
Occupationally-exposed lead affects the neuromuscular junction and might cause disturbances in the locomotor activity. This study was undertaken to evaluate pteridine metabolism, in which neurotransmitters are synthesized in battery workers. Urinary neopterin, biopterin and creatinine were measured using high performance liquid chromatography. Serum neopterin concentrations were detected by enzyme-linked immunoassay. Blood dihydropteridine reductase (DHPR) activities and deltaaminolevulinic acid (delta-ALA) were measured spectrophotometrically. Blood and urinary lead were detected by atomic absorption spectroscopy. Significantly increased blood and urinary lead levels, urinary neopterin, biopterin and delta-ALA were found in workers, while DHPR activities were indifferent compared to control group. Urinary creatinine decreased. This is the first study to demonstrate that increased activity of the pteridine pathway results in the accumulation of the neurotransmitters that may be responsible for the neurological disorders.
Engin
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Department of Pharmaceutical Toxicology, Faculty of Pharmacy, University of Hacettepe, Sihhiye, Ankara, Turkey.
Tuzun
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Sahin
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G
D000328:Adult; D000395:Air Pollutants, Occupational / Q000097:blood / Q000633:toxicity* / Q000652:urine; D000622:Aminolevulinic Acid / Q000097:blood; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001708:Biopterins / Q000378:metabolism / Q000652:urine*; D003404:Creatinine / Q000652:urine; D004093:Dihydropteridine Reductase / Q000097:blood / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D007854:Lead / Q000097:blood / Q000633:toxicity* / Q000652:urine; D008297:Male; D019798:Neopterin / Q000097:blood / Q000378:metabolism / Q000652:urine; D020511:Neuromuscular Junction Diseases / Q000097:blood / Q000378:metabolism / Q000652:urine; D016273:Occupational Exposure; D011621:Pteridines / Q000097:blood / Q000378:metabolism* / Q000652:urine
D000395:Air Pollutants, Occupational; D015415:Biomarkers; D011621:Pteridines; D001708:Biopterins; D007854:Lead; D019798:Neopterin; D000622:Aminolevulinic Acid; D003404:Creatinine; D004093:Dihydropteridine Reductase