Simultaneous saccharification and L-(+)-lactic acid fermentation of protease-treated wheat bran using mixed culture of lactobacilli.
Protease-treated wheat bran (20% w/v) of particle size less than 300 microm containing 65% (w/w) starch was used for the simultaneous saccharification and L-(+)-lactic acid fermentation by the mixed cultures of Lactobacillus casei and Lactobacillus delbrueckii. Maximum lactate yield after various process optimizations was 123 g l(-1) with a productivity of 2.3 g l(-1) h(-1) corresponding to a conversion of 0.95 g lactic acid per gram starch after 54 h at 37 degrees C. By using protease-treated wheat bran around tenfold decrease in supplementation of the costly medium component, like yeast extract, was achieved together with a considerable increase in the production level.
John
Rojan P
RP
Biotechnology Division, Regional Research Laboratory (CSIR), Trivandrum, 695 019, Kerala, India.
Nampoothiri
K Madhavan
KM
Pandey
Ashok
A
D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D004043:Dietary Fiber / Q000378:metabolism*; D005285:Fermentation; D007773:Lactates / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D007778:Lactobacillus / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry
D002241:Carbohydrates; D004043:Dietary Fiber; D007773:Lactates; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D010447:Peptide Hydrolases