title: Electrochemiluminescence determination of pipemidic acid using sulfite as energy transfer mediator.
abstract: An electrochemiluminescence (ECL) based on energy transfer from electro-generated triplet sulfur dioxide to pipemidic acid (PPA) was studied. A weak ECL from triplet sulfur dioxide (3)SO2 * was observed when sulfite was electrochemically oxidized in sulfuric acid solution on a Pt electrode. When PPA was present, the weak ECL was enhanced. The enhanced ECL was attributed to energy transfer from (3)SO2 * to PPA. Based on the enhanced ECL, a flow-injection (FIA) ECL method for the determination of PPA was proposed. The proposed method allowed the measurement of PPA over the range of 1.0x10(-7) to 2.0x10(-5)moll(-1). The detection limit was 3.9x10(-8)moll(-1), and the relative standard deviation for 1.0x10(-6)moll(-1) PPA (n=9) was 1.3%. This method was evaluated by the analysis of PPA in pharmaceutical preparations and urine samples.
authors.affiliation: Institute of Analytical Science, Northwest University, Xi'an 710069, PR China.
authors.forename: Yao-Dong
authors.initials: YD
authors.lastname: Liang
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Song
mesh_terms: D004735:Energy Transfer; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010876:Pipemidic Acid / Q000032:analysis* / Q000652:urine; D012680:Sensitivity and Specificity; D013447:Sulfites / Q000737:chemistry*; D013458:Sulfur Dioxide / Q000737:chemistry; D013997:Time Factors
chemical_list: D013447:Sulfites; D013458:Sulfur Dioxide; D010876:Pipemidic Acid