title: Hydrogen sulfide is an endogenous modulator of leukocyte-mediated inflammation.
abstract: Hydrogen sulfide (H2S) is increasingly recognized as an important signaling molecule in the cardiovascular and nervous systems. Recently, H2S donors were reported to induce neutrophil apoptosis and to suppress expression of some leukocyte and endothelial adhesion molecules. Using rats, we examined the possibility that H2S is an endogenous regulator of key inflammatory events at the leukocyte-endothelial interface. Via intravital microscopy, we observed that H2S donors (NaHS and Na2S) inhibited aspirin-induced leukocyte adherence in mesenteric venules (ED50 of 5.0 micromol/kg for Na2S), likely via activation of ATP-sensitive K+ (K(ATP)) channels. Inhibition of endogenous H2S synthesis elicited leukocyte adherence. Leukocyte infiltration in an air pouch model was also suppressed by H2S donors (NaHS, Lawesson's reagent, and N-acetylcysteine; ED50 of 42.7, 1.3, and 29.9 micromol/kg, respectively) and exacerbated by inhibition of endogenous H2S synthesis. Carrageenan-induced paw edema was suppressed by H2S donors (NaHS and Na2S; ED50s of 35 and 28 micromol/kg, respectively) to the same extent as by diclofenac and enhanced by an inhibitor of H2S synthesis. Suppression of edema formation by H2S donors was mimicked by a K(ATP) channel agonist and reversed by an antagonist of this channel. These results suggest that endogenous H2S is an important mediator of acute inflammation, acting at the leukocyte-endothelium interface. These findings have important implications for anti-inflammatory drug development.
authors.affiliation: Inflammation Research Network, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Renata C O
authors.initials: RC
authors.lastname: Zanardo
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Brancaleone
authors.forename: Eleonora
authors.initials: E
authors.lastname: Distrutti
authors.forename: Stefano
authors.initials: S
authors.lastname: Fiorucci
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cirino
authors.forename: John L
authors.initials: JL
authors.lastname: Wallace
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology*; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D007249:Inflammation; D018836:Inflammation Mediators / Q000494:pharmacology*; D007962:Leukocytes / Q000473:pathology*; D018715:Microscopy, Video; D051381:Rats; D013152:Splanchnic Circulation; D013440:Sulfides / Q000494:pharmacology; D014699:Venules
chemical_list: D018836:Inflammation Mediators; D013440:Sulfides; C025451:sodium bisulfide; D001241:Aspirin; C033479:sodium sulfide; D006862:Hydrogen Sulfide