title: B22 Glu des-B30 insulin: a novel monomeric insulin.
abstract: Studies on monomeric insulin with reduced self-association are important in the development of insulin pharmaceutical preparations with rapid hypoglycemic action on patients with diabetes. Here we report a novel monomeric insulin, B22 Glu des-B30 insulin, prepared from a single chain insulin precursor with B22 Arg mutated to Glu, which was expressed in Pichia pastoris and converted to B22 Glu des-B30 insulin by tryptic digestion. It still retains 50% of the in vivo biological activity of porcine insulin and does not form a dimer even at a concentration of 10 mg/ml, showing that B22 Glu plays a key role in reducing the self-association of the insulin molecule without greatly reducing its biological activity. This novel monomeric insulin might have potential applications in the clinic.
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Hai-Juan
authors.initials: HJ
authors.lastname: Du
authors.forename: Jia-Hao
authors.initials: JH
authors.lastname: Shi
authors.forename: Da-Fu
authors.initials: DF
authors.lastname: Cui
authors.forename: You-Shang
authors.initials: YS
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002942:Circular Dichroism; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D007328:Insulin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D010843:Pichia / Q000235:genetics; D011498:Protein Precursors / Q000378:metabolism; D011817:Rabbits
chemical_list: C513189:B22 Glu des-B30 insulin; D007004:Hypoglycemic Agents; D007328:Insulin; D011498:Protein Precursors