Alistipes onderdonkii sp. nov. and Alistipes shahii sp. nov., of human origin.
Two groups of previously unknown Gram-negative, strictly anaerobic, pigment-producing, rod-shaped bacteria, which phenotypically and phylogenetically displayed a close association with the recently described species Alistipes finegoldii, were characterized using phenotypic and molecular taxonomic methods. A 16S rRNA gene sequence divergence of approximately 3 % between the two unknown bacteria and A. finegoldii, as well as distinguishable biochemical characteristics, demonstrates that these organisms are genotypically and phenotypically distinct and that each group represents a previously unknown subline within the genus Alistipes. Chromosomal DNA-DNA reassociation studies further confirmed the separateness of the unidentified bacteria and A. finegoldii. On the basis of the phenotypic and phylogenetic findings, two novel species, Alistipes onderdonkii sp. nov. and Alistipes shahii sp. nov., are proposed. The type strains of A. onderdonkii and A. shahii are WAL 8169(T) (=CCUG 48946(T)=ATCC BAA-1178(T)) and WAL 8301(T) (=CCUG 48947(T)=ATCC BAA-1179(T)), respectively; their DNA G+C contents are 58 and 56 mol%, respectively.
Song
Yuli
Y
Research Service, VA Medical Center West Los Angeles, Building 304, Room E3-227, 11301 Wilshire Boulevard, Los Angeles, CA 90073, USA.
Könönen
Eija
E
Anaerobe Reference Laboratory, National Public Health Institute (KTL), Helsinki, Finland.
Rautio
Merja
M
Anaerobe Reference Laboratory, National Public Health Institute (KTL), Helsinki, Finland.
Liu
Chengxu
C
Research Service, VA Medical Center West Los Angeles, Building 304, Room E3-227, 11301 Wilshire Boulevard, Los Angeles, CA 90073, USA.
Bryk
Anne
A
Anaerobe Reference Laboratory, National Public Health Institute (KTL), Helsinki, Finland.
Eerola
Erkki
E
Department of Medical Microbiology, University of Turku, Turku, Finland.
Finegold
Sydney M
SM
Department of Microbiology, Immunology, and Molecular Genetics, UCLA School of Medicine, Los Angeles, CA, USA.|Department of Medicine, UCLA School of Medicine, Los Angeles, CA, USA.|Infectious Diseases Section, VA Medical Center West Los Angeles, Los Angeles, CA 90073, USA.
D041963:Bacteroidetes / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S