[Immune response of jackdaw (Corvus monedula) to antigens of Capillaria resecta (Nematoda)-western blotting analysis].
Biotinylated surface and somatic protein extracts of the nematode Capillaria resecta were analysed by SDS-PAGE followed by Western blotting in order to examine their antigenic character. The antigens were probing with serum of C. resecta naturally infected birds (jackdaws). Surface and somatic antigens were recognised by serum IgG, IgA and IgM antibodies. The most intense reactivity of sera was evident with TBS-soluble surface proteins. The present paper has shown that surface proteins of C. resecta are more immunogenic than somatic and they are able to elicit stronger immune response in the bird's body.
Frońska-Popiel
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Zakład Parazytologii Ogólnej, Instytut Mikrobiologii, Uniwersytet Wrocławski ul.Przybyszewskiego 63, 51-148 Wrocław.
Okulewicz
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Perec
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D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000276:immunology; D015153:Blotting, Western / Q000379:methods; D002195:Capillaria / Q000145:classification / Q000502:physiology*; D046368:Crows / Q000276:immunology* / Q000469:parasitology*; D006790:Host-Parasite Interactions; D007136:Immunoglobulins / Q000032:analysis* / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D007136:Immunoglobulins