title: Preparation of a single prostate needle biopsy specimen for histological diagnosis and RNA analysis.
abstract: OBJECTIVE
  When a prostate needle biopsy specimen is used for molecular study, a second specimen from the same area as the first one has previously been required for pathological diagnosis. However, we developed a method for obtaining substances such as RNA and we also made pathological diagnosis possible from a single needle biopsy specimen, improving reliability.
  
  
  METHODS
  Prostate needle biopsy specimens from 118 patients were frozen in optimal cutting temperature compound. Tissue from the upper part of the frozen specimen was sectioned longitudinally for histopathological examination. The remainder of the specimen was placed in TRIzol reagent to extract total RNA for molecular biological investigation.
  
  
  RESULTS
  Pathological diagnosis and total RNA extraction (1.6 to 32.7 microg) could be obtained from 1 needle biopsy specimen. When 2 specimens were obtained from the same area of the prostate, pathological diagnoses were discordant in 19% of the cases.
  
  
  CONCLUSIONS
  We established a method for histopathological diagnosis in the prostate needle biopsy specimen used for molecular investigation. This single specimen method may facilitate molecular research in prostate cancer.
authors.affiliation: Department of Urology, Dokkyo University School of Medicine, 880 Kitakobayashi, Mibu, Shimotsuga, Tochigi 321-0293, Japan. fukabori@dokkyomed.ac.jp
authors.forename: Yoshitatsu
authors.initials: Y
authors.lastname: Fukabori
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Yoshida
authors.forename: Katsuya
authors.initials: K
authors.lastname: Nakano
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Shibata
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Yamanaka
authors.forename: Tetsunari
authors.initials: T
authors.lastname: Oyama
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle; D006801:Humans; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology*; D012334:RNA, Neoplasm / Q000032:analysis*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D012334:RNA, Neoplasm