The genetic immunization with paraflagellar rod protein-2 fused to the HSP70 confers protection against late Trypanosoma cruzi infection.
The immunological properties of the Trypanosoma cruzi paraflagellar rod proteins (PFR2 and PFR3) administered alone as well as fused to HSP70 have been analyzed in mice in the context of genetic immunization. The immunization of mice with the DNA vectors containing the PFRs gene or PFRs-HSP70 fused genes induced high level of IgG(2a) anti-PFRs. However, only the immunization with the PFR2-HSP70 fused genes triggers in spleen cells a statistically significant enhancement of expression of IL-12 and IFN-gamma and a decrease in the percentage of cells expressing IL-4. Likewise, the PFR2-HSP70 molecule elicits a statistically significant activation of PFR2 antigen specific CTLs. Immunization with the PFR2-HSP70 chimeric gene provided a protective response against a T. cruzi experimental infection.
Morell
María
M
Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina López Neyra, CSIC, P.T. de Ciencias de la Salud. Avda del Conocimiento s/n, 18100-Granada, Spain.
Thomas
M Carmen
MC
Caballero
Trinidad
T
Alonso
Carlos
C
López
Manuel C
MC
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D049109:Cell Proliferation; D014355:Chagas Disease / Q000276:immunology* / Q000517:prevention & control*; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D016054:DNA, Protozoan / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
D016207:Cytokines; D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; C105582:PFR-2 protein, Leishmania; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic