title: [Cloning, identification, and cellular localization of a down-regulated gene fragment related with Down Syndrome].
abstract: OBJECTIVE
  To clone a novel gene and explore its expression patterns in tissues and cells, so as to find its role in the process of encephalopathy in DS.
  
  
  METHODS
  On the base of our previous microarray's result together with the tissue type, we chose EST AI480014 to carry out RACE, then analyzed its expression profiles in liver, spleen, kidney, heart, brain by multi-tissues Northern blot, after that semi-quantitive RT-PCR was used to reexamine the expression profiles. Furthermore, we used ISH to find whether aim gene expressed in neuroglial cells cultured in vitro. Finally we performed semi-quantitive RT-PCR to explore whether it expressed differently between DS and normal.
  
  
  RESULTS
  We gained a 682 bp new cDNA fragment (DQ275636) which expressed in all the tissues examined and had no alternative splices in them. It expressed highly in brain especially in frontal lobe and hippocampus. According to the ISH result we convinced that it expressed in neuroglial cells. Using bioinformatics we mapped DQ275636 to chromosome 5q14.
  
  
  CONCLUSIONS
  We have obtained a new gene fragment based on the above results. According to its expression character and tissue type, it can be suggested that this gene has a probable role in the process of encephalopathy in DS.
authors.affiliation: Department of Pediatrics, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Shang
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Yu
authors.forename: Rong
authors.initials: R
authors.lastname: Liang
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Jing-zhu
authors.initials: JZ
authors.lastname: Guo
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004314:Down Syndrome / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary