Valproate prevents MK801-induced changes in brain-derived neurotrophic factor mRNA in the rat brain.
To investigate whether the anticonvulsant valproate influences the changes in brain-derived neurotrophic factor (BDNF) mRNA expression induced by MK801 in rat brain, we injected valproate prior to MK801 and observed the changes in the BDNF expression 3 h later. MK801 significantly increased BDNF expression in the retrosplenial and entorhinal cortex, and these increases were prevented by valproate pretreatment. Valproate pretreatment significantly blocked the MK801-induced increase of BDNF expression in retrosplenial cortex at 3 h, 6 h, and 9 h after MK801 injection, suggesting that valproate pretreatment did not delay the MK801-induced increase of BDNF expression. However, MK801 significantly decreased BDNF expression in the granule cell layer of hippocampus, and valproate pretreatment before MK801 potentiated the MK801-induced decrease in BDNF expression in granule cell layer. These results indicate that valproate pretreatment differentially affects the MK801-induced changes in BDNF expression in a region-selective manner.
Jeon
Gye Sun
GS
Department of Pharmacology, Korea University College of Medicine, #5 Anam-dong, Seoul 136-705, South Korea.
Park
Sang-Ha
SH
Lee
Kuem-Ju
KJ
Lee
Min-Soo
MS
Chun
Boe-Gwun
BG
Shin
Kyung-Ho
KH
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014635:Valproic Acid / Q000494:pharmacology*
D000927:Anticonvulsants; D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D014635:Valproic Acid; D016291:Dizocilpine Maleate