Scale-up of recombinant Opc protein production in Escherichia coli for a meningococcal vaccine.
Opc is an outer membrane protein from Neisseria meningitidis present in meningococcal vaccine preparations. The opc gene, codifying for this protein, was cloned in to Escherichia coli and the Opc protein was expressed under the control of a tryptophan promoter. The recombinant strain was grown in batch cultures. Opc was expressed as inclusion bodies at about 32% of the total cellular protein. We examined the scale-up culture conditions for the production of the recombinant Opc. The scale-up process was performed from 1.5 l to 50 l culture, using first, the constant power per unit of volume (P/V) as main scaling criteria, and then the oxygen mass transfer coefficient (K(L)a) scaling criteria to adjust the optimal aeration conditions. A final productivity of 52 mgl(-1)h(-1) was obtained at the 50l culture scale compared with the 49 mgl(-1)h(-1) productivity at 1.5l laboratory scale.
Pérez
Raúl Espinosa
RE
División de Desarrollo Biofarmacéutico, Centro de Ingeniería Genética y Biotecnología, Ave 31e/ 158 y 190, Apdo. Postal 6162, Cubanacán, Playa, La Habana 10600, Cuba.
Lasa
Alexis Musacchio
AM
Rodríguez
Ricardo Silva
RS
Menéndez
Evelin Caballero
EC
Suárez
José García
JG
Balaguer
Héctor Díaz
HD
D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008585:Meningitis, Meningococcal / Q000517:prevention & control; D022401:Meningococcal Vaccines / Q000096:biosynthesis*; D008954:Models, Biological; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013678:Technology, Pharmaceutical / Q000379:methods*
D001425:Bacterial Outer Membrane Proteins; D022401:Meningococcal Vaccines; D011994:Recombinant Proteins; C074517:opc protein, bacteria