title: Proteomic analysis of recurrent joint inflammation in juvenile idiopathic arthritis.
abstract: The synovial fluid proteome in juvenile idiopathic arthritis was investigated to isolate joint-specific biomarkers that are expressed in patients displaying recurrent joint inflammation. To identify the synovial specific proteome, matched synovial fluid and plasma samples were subjected to protein separation by 2-dimension electrophoresis (2DE). Forty-three protein spots, overexpressed in the joint, were identified. Synovial fluids from children with single-event knee joint inflammation were then compared with a group with recurrent knee disease. Nine synovial specific proteins were significantly differentially expressed in the recurrent group. Proteolytic fragments of collagen X, fibrin beta-chain, and T-cell receptor alpha-region have been identified among this protein cluster. Putative biomarkers, overexpressed in the joint and differentially expressed in children with recurrent joint inflammation, have been identified. These proteins may play a significant role determining the pathological state within the chronically inflamed joint and influence disease progression in JIA. This is the first study of the synovial proteome in children.
authors.affiliation: Queen's University Belfast, Arthritis Research Group, Musculoskeletal Education and Research Unit, Musgrave Park Hospital, Belfast BT9 7JB, Northern Ireland. d.gibson@qub.ac.uk
authors.forename: David S
authors.initials: DS
authors.lastname: Gibson
authors.forename: Sarah
authors.initials: S
authors.lastname: Blelock
authors.forename: Simon
authors.initials: S
authors.lastname: Brockbank
authors.forename: Jim
authors.initials: J
authors.lastname: Curry
authors.forename: Adrienne
authors.initials: A
authors.lastname: Healy
authors.forename: Catherine
authors.initials: C
authors.lastname: McAllister
authors.forename: Madeleine E
authors.initials: ME
authors.lastname: Rooney
mesh_terms: D000293:Adolescent; D001171:Arthritis, Juvenile / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D002648:Child; D002675:Child, Preschool; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D007719:Knee Joint / Q000473:pathology*; D008297:Male; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D012008:Recurrence; D013582:Synovial Fluid / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D020543:Proteome