Chitinimonas koreensis sp. nov., isolated from greenhouse soil in Korea.
Strain R2A43-10(T) was isolated from a greenhouse soil in Korea. Cells were Gram-negative rods, motile by means of a single flagellum. Growth occurred at 10-40 degrees C and at pH 5-8. Ubiquinone-8 (Q-8) was the only respiratory lipoquinone. Major fatty acids were summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH) and C(16 : 0). 16S rRNA gene sequence analysis revealed that strain R2A43-10(T) was closely related to Chitinimonas taiwanensis cf(T) (sequence similarity of 94.8 %), but it exhibited low sequence similarities (<92 %) to other members of the Betaproteobacteria. The G+C content of the genomic DNA of strain R2A43-10(T) was 65.0 mol%. The novel isolate could be differentiated from C. taiwanensis cf(T) by several physiological properties. On the basis of genomic and phenotypic data, it is concluded that R2A43-10(T) (=KACC 11467(T)=DSM 17726(T)) is the type strain of a novel species of the genus Chitinimonas, for which the name Chitinimonas koreensis sp. nov. is proposed.
Kim
Byung-Yong
BY
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon, Republic of Korea.
Weon
Hang-Yeon
HY
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon, Republic of Korea.
Yoo
Seung-Hee
SH
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon, Republic of Korea.
Chen
Wen-Ming
WM
Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan.
Kwon
Soon-Wo
SW
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon, Republic of Korea.
Go
Seung-Joo
SJ
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon, Republic of Korea.
Stackebrandt
Erko
E
Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
D001482:Base Composition; D020562:Betaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D013696:Temperature
D003470:Culture Media; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S