Anti-CHH antibody causes impaired hyperglycemia in Penaeus monodon.
Crustacean hyperglycemic hormone (CHH) plays a major role in controlling glucose level in the haemolymph and also triggers important events during molting and reproductive cycles. In Penaeus monodon, three types of CHH, namely Pem-CHH1, Pem-CHH2 and Pem-CHH3, have been previously characterized. In this study, mouse polyclonal antibody was raised against recombinant Pem-CHH1 that was expressed in Escherichia coli. The anti-Pem-CHH1 antibody recognized all three types of Pem-CHHs but did not cross-react with either related hormone, molt-inhibiting hormone of P. monodon, or unrelated human growth hormone. The hyperglycemic activity in the extract from the eyestalk neural tissues was significantly depleted after incubating with anti-Pem-CHH antibody. Direct injection of the antibody into shrimp caused about 30-50% reduction in the haemolymph glucose level. The result demonstrates the ability of anti-Pem-CHH1 antibody to deplete the activity of CHH in vivo, and thus provides a possibility of using anti-Pem-CHH1 antibody to inhibit the hormone activity as a strategy to modulate growth and reproduction in this species.
Treerattrakool
Supattra
S
Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand.
Udomkit
Apinunt
A
Panyim
Sakol
S
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D060829:Arthropod Proteins; D001786:Blood Glucose / Q000378:metabolism*; D005260:Female; D006458:Hemolymph; D007447:Invertebrate Hormones; D051379:Mice; D009419:Nerve Tissue Proteins / Q000276:immunology*; D033561:Penaeidae / Q000378:metabolism*; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity
D000906:Antibodies; D060829:Arthropod Proteins; D001786:Blood Glucose; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C015947:hyperglycemic hormone, crustacean