M gene analysis of atypical strains of feline and canine coronavirus circulating in an Austrian animal shelter.
Coronavirus-positive samples of faeces collected in an Austrian animal shelter from 12 cats and 10 dogs were analysed by reverse transcriptase-pcr with primers amplifying a segment of the M protein gene, and by sequence analysis. In addition, the samples were subjected to S gene typing, using primers that differentiated between feline coronavirus (fcov) types I and II. A phylogenetic analysis of the M gene sequences revealed not only clearly segregating canine coronavirus (ccov) in the dogs, typical ccov sequences and the recently described fcov-like ccov, but also at least two genetic clusters of fcov in the cats, one species-specific, the other more closely related to fcov-like ccov. The M gene sequences of these new feline strains had at most 88 per cent identity with the fcov-like ccov strain 259/01 and only up to 85 per cent with any fcov sequence available in GenBank. In the phylogenetic tree they occupy an intermediate position between feline and canine coronaviruses.
Benetka
V
V
Clinical Virology, Department of Diagnostic Imaging, Infectious Diseases and Clinical Pathology, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.
Kolodziejek
J
J
Walk
K
K
Rennhofer
M
M
Möstl
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001317:Austria / Q000453:epidemiology; D002371:Cat Diseases / Q000453:epidemiology / Q000821:virology*; D002415:Cats; D017934:Coronavirus / Q000145:classification* / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D000086862:Coronavirus M Proteins; D017939:Coronavirus, Canine / Q000145:classification / Q000302:isolation & purification; D016765:Coronavirus, Feline / Q000145:classification / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis; D004283:Dog Diseases / Q000453:epidemiology / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014763:Viral Matrix Proteins / Q000032:analysis*
D000086862:Coronavirus M Proteins; D004279:DNA, Viral; D012367:RNA, Viral; D014763:Viral Matrix Proteins