title: In vivo commitment to yeast cotranscriptional splicing is sensitive to transcription elongation mutants.
abstract: Spliceosome assembly in the budding yeast Saccharomyces cerevisiae was recently shown to occur at the site of transcription. However, evidence for cotranscriptional splicing as well as for coupling between transcription and splicing is still lacking. Using modifications of a previously published chromatin immunoprecipitation (ChIP) assay, we show that cotranscriptional splicing occurs approximately 1 kb after transcription of the 3' splice site (3'SS). This pathway furthermore protects most intron-containing nascent transcripts from the effects of cleavage by an intronic hammerhead ribozyme. This suggests that a high percentage of introns are recognized cotranscriptionally. This observation led us to screen a small deletion library for strains that sensitize a splicing reporter to ribozyme cleavage. Characterization of the Deltamud2 strain indicates that the early splicing factor Mud2p functions with U1 snRNP to form a cross-intron bridging complex on nascent pre-mRNA. The complex helps protect the transcript from ribozyme-mediated destruction and suggests an intron-definition event early in the spliceosome assembly process. The transcription elongation mutant strains Deltadst1 and Deltapaf1 show different cotranscriptional splicing phenotypes, suggesting that different transcription pathways differentially impact the efficiency of nascent intron definition.
authors.affiliation: Howard Hughes Medical Institute, Biology Department, Brandeis University, Waltham, Massachusetts 02454, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Lacadie
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Tardiff
authors.forename: Sebastian
authors.initials: S
authors.lastname: Kadener
authors.forename: Michael
authors.initials: M
authors.lastname: Rosbash
mesh_terms: D047369:Chromatin Immunoprecipitation; D007438:Introns / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D012326:RNA Splicing; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D017381:Spliceosomes; D000072668:Splicing Factor U2AF; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: C089980:MUD2 protein, S cerevisiae; D016337:RNA, Catalytic; D017412:Ribonucleoprotein, U1 Small Nuclear; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins; D000072668:Splicing Factor U2AF; C427204:hammerhead ribozyme; D001616:beta-Galactosidase