title: Poor recognition of HIV-1 Nef protein by CD8 T cells from HIV-1-infected children: impact of age.
abstract: Recognition of various HIV proteins by CD8 T cells from HIV-infected children was determined by two functional assays. First, using an Elispot assay, we show that 80% of patients recognized Gag, 77% recognized Pol, 61% recognized Env, 44% recognized Nef and 29% recognized Vif. Frequencies of Gag-, Pol-, and Env-specific IFN-gamma producing CD8 T cells were higher than frequencies of Nef and Vif-specific CD8 T cells. The poor recognition of Nef by ex vivo CD8 T cells was confirmed by CTL assays performed in HAART naïve children: 25% of children had positive response against Nef versus 44, 63 and 62% for Env, Gag, and Pol, respectively. Memory Gag-specific CTL were positively correlated with age, whereas Nef-specific CTL were negatively correlated with age. The poor Nef-specific CD8 T cell response in HIV-infected children contrasts with dominance of Nef-specific responses in infected adults.
authors.affiliation: Unité Postulante d'Immunopathologie Virale, URA CNRS 1930, Institut Pasteur, Bat Lwoff, 28 rue du Dr Roux, 75015 Paris, France. florence@pasteur.fr
authors.forename: Florence
authors.initials: F
authors.lastname: Buseyne
authors.forename: Daniel
authors.initials: D
authors.lastname: Scott-Algara
authors.forename: Béatrice
authors.initials: B
authors.lastname: Corre
authors.forename: Françoise
authors.initials: F
authors.lastname: Porrot
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Monchatre
authors.forename: Nassima
authors.initials: N
authors.lastname: Bellal
authors.forename: Marianne
authors.initials: M
authors.lastname: Burgard
authors.forename: Christine
authors.initials: C
authors.lastname: Rouzioux
authors.forename: Stéphane
authors.initials: S
authors.lastname: Blanche
authors.forename: Yves
authors.initials: Y
authors.lastname: Rivière
mesh_terms: D000367:Age Factors; D019380:Anti-HIV Agents / Q000627:therapeutic use; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002675:Child, Preschool; D015693:Gene Products, nef / Q000276:immunology / Q000378:metabolism*; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007223:Infant; D012189:Retrospective Studies; D054311:nef Gene Products, Human Immunodeficiency Virus
chemical_list: D019380:Anti-HIV Agents; D015693:Gene Products, nef; D054311:nef Gene Products, Human Immunodeficiency Virus