title: Comparison of real-time PCR assays for monitoring serum hepatitis B virus DNA levels during antiviral therapy.
abstract: The performance characteristics of the RealART and Molecular Beacons assays were compared with those of the Digene Hybrid Capture II assay (ultrasensitive). The results of the RealART and Digene Hybrid assays were related (r = 0.94; P < 0.001) and diverged by 2 orders of magnitude. The RealART assay can be used to effectively monitor serum hepatitis B virus DNA levels.
authors.affiliation: Department of Medicine, University of Hong Kong, Hong Kong, SAR, China.
authors.forename: Chee-Kin
authors.initials: CK
authors.lastname: Hui
authors.forename: Scott
authors.initials: S
authors.lastname: Bowden
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Zhang
authors.forename: Anita
authors.initials: A
authors.lastname: Wong
authors.forename: Sharon
authors.initials: S
authors.lastname: Lewin
authors.forename: Frank
authors.initials: F
authors.lastname: Rousseau
authors.forename: Herve
authors.initials: H
authors.lastname: Mommeja-Marin
authors.forename: Nikki P
authors.initials: NP
authors.lastname: Lee
authors.forename: John M
authors.initials: JM
authors.lastname: Luk
authors.forename: Stephen
authors.initials: S
authors.lastname: Locarnini
authors.forename: Nancy
authors.initials: N
authors.lastname: Leung
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Naoumov
authors.forename: George K K
authors.initials: GK
authors.lastname: Lau
mesh_terms: D004279:DNA, Viral / Q000032:analysis*; D016903:Drug Monitoring / Q000379:methods*; D006509:Hepatitis B / Q000188:drug therapy* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D004279:DNA, Viral