Identification of mobile cholesterol compounds in experimental gliomas by (1)H MRS in vivo: effects of ganciclovir-induced apoptosis on lipids.
This letter presents a novel identification and analysis of mobile cholesterol compounds in an experimental glioma model by (1)H MRS in vivo. The cholesterol compounds turned out to comprise as much as 17 mol% of MRS visible total lipids. The results also imply partly associated accumulation of (1)H MRS detectable cholesterol compounds and unsaturated lipids during gene therapy-induced apoptosis, and indicate that the contribution of cholesterol compounds cannot be bypassed in spectral lipid analysis. The introduced (1)H MRS approach facilitates a non-invasive follow-up of mobile cholesterol compounds, paving way for studies of tumour cholesterol metabolism in vivo.
Liimatainen
Timo
T
A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O. Box 1627, FI-70211 Kuopio, Finland. timo.liimatainen@uku.fi
Lehtimäki
Kimmo
K
Ala-Korpela
Mika
M
Hakumäki
Juhana
J
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D005260:Female; D015774:Ganciclovir / Q000494:pharmacology*; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009368:Neoplasm Transplantation; D051381:Rats
D000998:Antiviral Agents; D002784:Cholesterol; D015774:Ganciclovir