title: Pyrosequencing as a rapid tool for identification of GES-type extended-spectrum beta-lactamases.
abstract: A pyrosequencing technique was used for identification of extended-spectrum beta-lactamases (ESBLs) of GES type. These beta-lactamases are isolated increasingly emerging in gram-negative bacteria worldwide. This rapid and reliable identification method is interesting, since GES variants, including not only expanded-spectrum cephalosporins but also carbapenems, cephamycins, and monobactams, are the only ESBLs that possess different hydrolysis profiles.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, 94275 K.-Bicêtre, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Thierry
authors.initials: T
authors.lastname: Naas
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D001426:Bacterial Proteins / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D001618:beta-Lactamases / Q000145:classification* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases