title: Usefulness of dura mater in providing DNA samples for identifying cadavers.
abstract: We examined the usefulness of the dura mater in identifying human remains. Dura mater was collected from 50 cadavers, including drowned, charred, and mummified remains. The STR genotype using the AmpFlSTR Identifiler Kit could be typed at 15 STR and amelogenin loci in 30 samples of 33 cases. Furthermore, the ABO genotype and amelogenin using gel-based methods could be typed in 44 samples of 50 cases. In cases with successful typing of STR, ABO-DNA, and amelogenin, the longest time after death was from 12 to 26 days in a drowned body. The minimum quantity of dura mater required for DNA extraction was about 2.5 mg, dried and fixed by ethanol, in a cadaver 15 h after death. The state of the DNA from the dura mater from the calvaria may be better than that from the basis cranii interna. We found that DNA from dura mater is one of the most useful samples for forensic identification.
authors.affiliation: Department of Legal Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. hms1466@faculty.chiba-u.jp
authors.forename: Hisako
authors.initials: H
authors.lastname: Motani
authors.forename: Koichi
authors.initials: K
authors.lastname: Sakurada
authors.forename: Tomoko
authors.initials: T
authors.lastname: Akutsu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikegaya
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Hayakawa
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Sato
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yajima
authors.forename: Kaoru
authors.initials: K
authors.lastname: Sato
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Hirotaro
authors.initials: H
authors.lastname: Iwase
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D053523:Amelogenin; D002102:Cadaver; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D003746:Dental Enamel Proteins / Q000235:genetics; D004388:Dura Mater / Q000473:pathology*; D005260:Female; D018732:Forensic Anthropology / Q000379:methods*; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D019849:Sex Determination Processes; D020080:Tandem Repeat Sequences
chemical_list: D000017:ABO Blood-Group System; D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA