Biotinylated glycopolymers synthesized by atom transfer radical polymerization.
Biotinylated glycopolymers that bind to the protein streptavidin were synthesized by atom transfer radical polymerization (ATRP). Poly(methacrylate)s with pendent N-acetyl-d-glucosamines were prepared by polymerizing the protected monomer, followed by deprotection. Alternatively, the unprotected monomer was directly polymerized. Both paths provided well-defined glycopolymers with narrow molecular weight distributions (PDI = 1.07-1.23). The number-average molecular weights determined by gel permeation chromatography increased with increasing initial monomer-to-initiator ratios. The polymers were synthesized using a biotin-functionalized initiator for ATRP. Confirmation of the end group and binding to the protein streptavidin was achieved by (1)H NMR and surface plamon resonance.
Vázquez-Dorbatt
Vimary
V
Department of Chemistry and Biochemistry and California Nanosystems Institute, University of California, Los Angeles, 607 Charles E. Young Drive East, Los Angeles, California 90095-1569, USA.
Maynard
Heather D
HD
D000117:Acetylglucosamine / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011485:Protein Binding; D019809:Streptavidin / Q000737:chemistry*; D020349:Surface Plasmon Resonance
D011108:Polymers; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D001710:Biotin; D019809:Streptavidin; D000117:Acetylglucosamine