title: No association of DNA ligase-I polymorphism with the risk of lung cancer in north-Indian population.
abstract: DNA ligases play an essential role in repair, replication, and recombination of DNA, and catalyzes the formation of a phosphodiester bond at a nick junction on single- and double-strand breaks. We have conducted a hospital-based case-control study to examine the role of polymorphism of DNA repair gene ligase I (LIGI) in the context of lung cancer risk for north Indian population. One hundred, fifty-one primary lung cancer cases and an equal number of matching hospital controls were collected. The LIGI polymorphism was determined by using the PCR-RFLP method. The association between polymorphisms in the LIGI gene with the risk of lung cancer was estimated by computing odds ratios (ORs) and a 95% confidence interval (CI) using a Multivariate Logistic Regression Analysis. The risk for lung cancer was not associated for individuals featuring LIGI (AC) (OR -0.8, 95% CI = 0.44-1.40) and (AA) (OR -0.8, 95% CI = 0.41-1.80) genotypes. The DNA repair gene (LIGI) may not be playing an important role in modulating the risk of lung cancer in the north Indian population.
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh, India. rcsobti@pu.ac.in
authors.forename: Ranbir Chander
authors.initials: RC
authors.lastname: Sobti
authors.forename: Pushpinder
authors.initials: P
authors.lastname: Kaur
authors.forename: Satinder
authors.initials: S
authors.lastname: Kaur
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Janmeja
authors.forename: Surinder Kumar
authors.initials: SK
authors.lastname: Jindal
authors.forename: Jai
authors.initials: J
authors.lastname: Kishan
authors.forename: Sara
authors.initials: S
authors.lastname: Raimondi
mesh_terms: D000368:Aged; D001483:Base Sequence; D016022:Case-Control Studies; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000378:metabolism*; D017931:DNA Primers; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007194:India; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D017931:DNA Primers; C000607892:LIG1 protein, human; D011088:DNA Ligases; D000072481:DNA Ligase ATP