Aggregation studies of the water-soluble gadofullerene magnetic resonance imaging contrast agent: [Gd@C82O6(OH)16(NHCH2CH2COOH)8]x.
The aggregation behavior of the newly synthesized gadofullerene magnetic resonance imaging (MRI) contrast agent, i.e., Gd@C(82)O(6)(OH)(16)(NHCH(2)CH(2)COOH)(8) (abbreviated as AAD-EMF), was studied in detail by dynamic light scattering, scanning electron microscopy, T(1)-weighted magnetic resonance, and atomic force microscopy. It was revealed that the AAD-EMF aggregation in aqueous solution is pH-dependent. At pH 2, the AAD-EMF first self-assemble to form ca. 30 nm small clusters, and then dozens of the small clusters further aggregate to form large grapelike particles. At pH 7, the aggregates are also ca. 30 nm small clusters, but they are hard to further aggregate except for forming some cluster dimers or trimers, so AAD-EMF aggregates have a narrow size distribution by this time. At pH 9, the AAD-EMF aggregations cover a large range of continuous hydrodynamic diameters from 30 to 2000 nm. On the basis of the above observations, the aggregating mechanism of AAD-EMF under different pH values was proposed by concurrently considering the hydrogen-bonding effect and the dipolar interactions between AAD-EMF.
Shu
Chun-Ying
CY
Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Zhang
Er-Yun
EY
Xiang
Jun-Feng
JF
Zhu
Chuan-Feng
CF
Wang
Chun-Ru
CR
Pei
Xin-Long
XL
Han
Hong-Bin
HB
D003287:Contrast Media / Q000737:chemistry*; D037741:Fullerenes / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008279:Magnetic Resonance Imaging; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size; D012995:Solubility; D014867:Water / Q000737:chemistry
D003287:Contrast Media; D037741:Fullerenes; D009942:Organometallic Compounds; D014867:Water; D005682:Gadolinium