BRAK/CXCL14 expression suppresses tumor growth in vivo in human oral carcinoma cells.
In order to find a suppressor(s) of tumor progression in vivo for oral carcinoma (OC), we searched for molecules down-regulated in OC cells when the cells were treated with epidermal growth factor (EGF), whose receptor is frequently over-activated in OC. The expression of BRAK, which is also known as CXC chemokine ligand14 (CXCL14), was down-regulated significantly by the treatment of OC cells with EGF as observed by cDNA microarray analysis followed by reverse-transcriptase polymerase chain reaction analysis. The EGF effect was attenuated by the co-presence of a MEK inhibitor. The rate of tumor formation in vivo of BRAK-expressing vector-transfected tumor cells in athymic nude mice was significantly lower than that of mock vector-transfected ones. In addition tumors formed in vivo by the BRAK-expressing cells were significantly smaller than those of the mock-transfected ones. These results indicate that BRAK/CXCL14 is a chemokine, having suppressive activity toward tumor progression of OC in vivo.
Ozawa
Shigeyuki
S
Department of Biochemistry and Molecular Biology, 82 Inaoka-cho, Yokosuka, 238-8580, Japan.
Kato
Yasumasa
Y
Komori
Reika
R
Maehata
Yojiro
Y
Kubota
Eiro
E
Hata
Ryu-Ichiro
R
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D045744:Cell Line, Tumor; D019743:Chemokines, CXC / Q000502:physiology* / Q000627:therapeutic use; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors / Q000502:physiology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D015398:Signal Transduction; D014062:Tongue Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000502:physiology
C118056:CXCL14 protein, human; D019743:Chemokines, CXC; D025521:Tumor Suppressor Proteins; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D048049:Extracellular Signal-Regulated MAP Kinases