title: Aminobacterium colombiensegen. nov. sp. nov., an amino acid-degrading anaerobe isolated from anaerobic sludge.
abstract: A new gram-negative, non-sporulating, mesophilic, amino acid fermenting bacterium, designated strain ALA-1(T) (T = type strain), was isolated from an anaerobic lagoon of a dairy wastewater treatment plant. The strain is curved (3-4 microm x 0.2-0.3 microm) and occurs singly or in pairs. Optimum growth occurs at 37 degrees C and pH 7.3. The G+C content of the DNA is 46 mol %. The strain requires yeast extract for growth, grows poorly on casamino acids, peptones, cysteine, and alpha-ketoglutarate, but readily grows on serine, threonine, glycine and pyruvate. When cocultured with the hydrogenotrophic methanogen Methanobacterium formicicum, strain ALA-1(T) oxidized alanine, glutamate, leucine, isoleucine, valine, aspartate, and methionine. Phylogenetic analysis revealed that it forms a distinct and independent line of descent in the vicinity of Dethiosulfovibrio peptidovorans, Dictyoglomus thermophilum, and Anaerobaculum thermoterrenum which are members of the low G+C containing gram-positive bacteria. The phylogenetic results concur with the phenotypic and genomic data which reveal that it is a novel strain. Based on these findings, we designate strain ALA-1(T) as Aminobacterium colombiense (DSM 12261) gen. nov., sp. nov.
authors.affiliation: Laboratoire ORSTOM de Microbiologiedes Anaérobies, Université de Provence, CESB/ESIL Case 925, 163 Avenue de Luminy, 13288, Marseille, Cedex 09, France.
authors.forename: S
authors.initials: S
authors.lastname: Baena
authors.forename: M L
authors.initials: ML
authors.lastname: Fardeau
authors.forename: M
authors.initials: M
authors.lastname: Labat
authors.forename: B
authors.initials: B
authors.lastname: Ollivier
authors.forename: P
authors.initials: P
authors.lastname: Thomas
authors.forename: J L
authors.initials: JL
authors.lastname: Garcia
authors.forename: B K
authors.initials: BK
authors.lastname: Patel