Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis.
A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients with culture-confirmed melioidosis. All six patients with melioidosis septic shock were blood PCR positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy.
Meumann
Ella M
EM
Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.
Novak
Ryan T
RT
Gal
Daniel
D
Kaestli
Mirjam E
ME
Mayo
Mark
M
Hanson
Joshua P
JP
Spencer
Emma
E
Glass
Mindy B
MB
Gee
Jay E
JE
Wilkins
Patricia P
PP
Currie
Bart J
BJ
D001692:Biological Transport / Q000235:genetics; D001769:Blood / Q000382:microbiology; D001826:Body Fluids / Q000382:microbiology; D016957:Burkholderia pseudomallei / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D019353:Endemic Diseases; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000382:microbiology
D004269:DNA, Bacterial