title: Intraspecific diversity of Oenococcus oeni strains determined by sequence analysis of target genes.
abstract: Using molecular techniques and sequencing, we studied the intraspecific diversity of Oenococcus oeni, a lactic acid bacterium involved in red winemaking. A relationship between the phenotypic and genotypic characterization of 16 O. oeni strains isolated from wine with different levels of enological potential was shown. The study was based on the comparative genomic analysis by subtractive hybridization between two strains of O. oeni with opposite enological potential. The genomic sequences obtained from subtractive hybridization were amplified by polymerase chain reaction and sequenced for the 16 strains. A considerable diversity among strains of O. oeni was observed.
authors.affiliation: Faculte d'Oenologie, UMR 1219, Oenologie-Ampelologie, INRA-Universite Victor Segalen Bordeaux 2, 351 cours de la Liberation, Talence 33405, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Delaherche
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Bon
authors.forename: Aurelien
authors.initials: A
authors.lastname: Dupé
authors.forename: Megumi
authors.initials: M
authors.lastname: Lucas
authors.forename: Benoit
authors.initials: B
authors.lastname: Arveiler
authors.forename: Antoine
authors.initials: A
authors.lastname: De Daruvar
authors.forename: Aline
authors.initials: A
authors.lastname: Lonvaud-Funel
mesh_terms: D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000378:metabolism; D014644:Genetic Variation; D016678:Genome; D005838:Genotype; D007777:Lactobacillaceae / Q000235:genetics* / Q000502:physiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial