Brooklawnia cerclae gen. nov., sp. nov., a propionate-forming bacterium isolated from chlorosolvent-contaminated groundwater.
Two novel facultatively anaerobic bacterial strains, BL-34(T) and BL-35, isolated from groundwater contaminated by a mixture of chlorosolvents were characterized using a polyphasic approach. The two strains exhibited essentially identical taxonomic features except for a vitamin B(12) requirement by strain BL-35 for optimal growth. Phylogenetically, the isolates were affiliated with members of the family Propionibacteriaceae and were placed in a phylogenetic branch adjacent to, but distinct from, those of the genera Propionimicrobium, Propionibacterium, Luteococcus, Propioniferax and Tessaracoccus. The cells of the novel strains were Gram-positive, non-motile, non-spore-forming pleomorphic rods. They produced catalase but not oxidase, and nitrate reduction did not occur in peptone/yeast extract/glucose medium. Propionate and acetate were the predominant products of glucose fermentation. Fermentation occurred in the presence of 1,2-dichloroethane and 1,1,2-trichloroethane at concentrations up to at least 9.8 mM. The genomic DNA G+C content was 67.5-67.9 mol%. Menaquinone MK-9(H(4)) was the predominant respiratory quinone and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer. The major cellular fatty acids were C(15 : 0) and anteiso-C(15 : 0). On the basis of the results obtained in this study, strains BL-34(T) and BL-35 should be classified within a novel taxon, for which the name Brooklawnia cerclae gen. nov., sp. nov. is proposed. The type strain of Brooklawnia cerclae is BL-34(T) (=LMG 23248(T)=NRRL B-41418(T)). An additional strain, BL-35 (=LMG 23249=NRRL B-41419), was also characterized.
Bae
Hee-Sung
HS
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Moe
William M
WM
Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Yan
Jun
J
Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Tiago
Igor
I
Departamento de Zoologia and Centro de Neurociências, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
da Costa
Milton S
MS
Departamento de Bioquímica and Centro de Neurociências, Universidade de Coimbra, 3001-401 Coimbra, Portugal.
Rainey
Fred A
FA
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
D039903:Actinobacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D001482:Base Composition; D002374:Catalase; D002713:Chlorine; D003470:Culture Media; D003487:Cyanoacrylates / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry; D003960:Diaminopimelic Acid / Q000032:analysis; D005025:Ethylene Dichlorides / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation / Q000187:drug effects; D016680:Genome, Bacterial; D005947:Glucose / Q000378:metabolism; D010461:Peptones; D010802:Phylogeny; D011422:Propionates / Q000378:metabolism*; D012997:Solvents; D014240:Trichloroethanes / Q000494:pharmacology; D024482:Vitamin K 2 / Q000031:analogs & derivatives; D014873:Water Pollutants
D003470:Culture Media; D003487:Cyanoacrylates; D004269:DNA, Bacterial; D005025:Ethylene Dichlorides; D005227:Fatty Acids; D010461:Peptones; D011422:Propionates; D012997:Solvents; D014240:Trichloroethanes; D014873:Water Pollutants; D024482:Vitamin K 2; C007166:cyacrin; C024567:1,1,2-trichloroethane; D002713:Chlorine; C073355:menaquinone 9; C024565:ethylene dichloride; D003960:Diaminopimelic Acid; D002374:Catalase; D005947:Glucose