[Proliferative activity parameters and their correlation with genetic damage of blood lymphocytes during cultivation under the conditions of cytokinetic block].
The subjects of the study were 15 volunteers aged 22 to 25 years, who underwent 25 air ionization sessions. The effects of genome instability were evaluated, and correlations between indicators of genome damage (lesions of micronuclei and nucleoplasmatic bridges) and parameters of proliferative and replicative activity (mitotic index, proliferative pool, the fraction of rapidly dividing cells, and replication index) of blood lymphocytes in the culture were studied. In order to establish the associations between the parameters, the parallel cultures were exposed to 0.07 mM of the standard mutagen MNNG during 5 hours. The study showed that the course of air ionization did not induce the micronuclei and nucleoplasmatic bridges in binuclear cells, but increased proliferative cell activity. This effect was accompanied by an increase in the fraction of rapidly dividing cells among all the dividing cells, and an increase in the dispersion of all proliferation parameters. MNNG induced a constant level of micronuclei in binuclear cells during the whole course, but not before the beginning of air ionization. The changes in the parameter "the fraction of dividing cells" (proliferative pool) were the most prominent manifestation of the suppression of proliferation by MNNG. MNNG loading inhibited the formation of binuclear cells most of all. The results demonstrate a non-random character of the correlation between the level of micronuclei in binuclear cells and proliferative activity parameters during cell cultivation under the conditions of cytokinetic block.
Ingel'
F I
FI
Iurchenko
V V
VV
Gus'kov
A S
AS
Krivtsova
E K
EK
Iurtseva
N A
NA
D000328:Adult; D000390:Air Ionization; D049109:Cell Proliferation; D002869:Chromosome Aberrations; D003571:Cytochalasin B / Q000008:administration & dosage / Q000009:adverse effects; D016207:Cytokines / Q000187:drug effects / Q000235:genetics* / Q000276:immunology*; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology*; D008297:Male
D016207:Cytokines; D005819:Genetic Markers; D003571:Cytochalasin B