Polymorphic and sex-limited phosphoglucomutase in Parastrongylus cantonensis (Nematoda: Angiostrongylidae).
Phosphoglucomutase was studied by polyacrylamide gel electrophoresis in the Thailand and Hawaii isolates of Parastrongylus cantonensis (also known as Angiostrongylus cantonensis). Two loci were present. The faster-moving locus (PGM-1) was polymorphic in the Hawaii isolate, represented by two alleles - the faster-moving, less common Pgm-1A and the slower-moving, more common Pgm-1B . It was monomorphic for the faster-moving allele Pgm-1A in the Thailand isolate. The slower-moving locus (PGM-2) was invariant, with a single band of enzyme activity, in the female worms of both the Thailand and Hawaii isolates. There was no detectable enzyme activity at this PGM-2 locus in the male worms of both isolates. The non-expression or 'null' PGM-2 phenotype in the male worms was presumed to be sex- limited. The present findings differ significantly in several aspects (polymorphic locus, proportion of polymorphic loci, heterozygosity, deviations from Hardy-Weinberg expectations, sex-limited expression) from the Japan isolate of P. cantonensis reported in the literature.
Praphathip
Eamsobhana
E
Department of Parasitology, Faculty of Medicine Siriraj Hospital.
Naowarat
Dechkum
D
Yong
Hoi-Sen
HS
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005838:Genotype; D006254:Hawaii; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism; D006579:Heterozygote; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D010733:Phosphoglucomutase / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D051381:Rats; D012737:Sex Factors; D013785:Thailand
D015801:Helminth Proteins; D007527:Isoenzymes; D010733:Phosphoglucomutase