Proteomic analysis of recurrent joint inflammation in juvenile idiopathic arthritis.
The synovial fluid proteome in juvenile idiopathic arthritis was investigated to isolate joint-specific biomarkers that are expressed in patients displaying recurrent joint inflammation. To identify the synovial specific proteome, matched synovial fluid and plasma samples were subjected to protein separation by 2-dimension electrophoresis (2DE). Forty-three protein spots, overexpressed in the joint, were identified. Synovial fluids from children with single-event knee joint inflammation were then compared with a group with recurrent knee disease. Nine synovial specific proteins were significantly differentially expressed in the recurrent group. Proteolytic fragments of collagen X, fibrin beta-chain, and T-cell receptor alpha-region have been identified among this protein cluster. Putative biomarkers, overexpressed in the joint and differentially expressed in children with recurrent joint inflammation, have been identified. These proteins may play a significant role determining the pathological state within the chronically inflamed joint and influence disease progression in JIA. This is the first study of the synovial proteome in children.
Gibson
David S
DS
Queen's University Belfast, Arthritis Research Group, Musculoskeletal Education and Research Unit, Musgrave Park Hospital, Belfast BT9 7JB, Northern Ireland. d.gibson@qub.ac.uk
Blelock
Sarah
S
Brockbank
Simon
S
Curry
Jim
J
Healy
Adrienne
A
McAllister
Catherine
C
Rooney
Madeleine E
ME
D000293:Adolescent; D001171:Arthritis, Juvenile / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D002648:Child; D002675:Child, Preschool; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D007719:Knee Joint / Q000473:pathology*; D008297:Male; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D012008:Recurrence; D013582:Synovial Fluid / Q000737:chemistry*
D015415:Biomarkers; D020543:Proteome