Differentiation of spontaneous and induced mammary adenocarcinomas of the rat by gene expression profiling.
The differentiation of spontaneous and induced adenocarcinomas is of interest in the setting of carcinogenicity studies. In the experiment reported here, a differentiation of morphologically similar spontaneous and induced mammary adenocarcinomas of the rat is achieved by gene expression profiling. By choosing one marker gene based on the gene expression profile for each tumour type, we were able to distinguish the tumours by real-time quantitative polymerase chain reaction (RT-QPCR) as well.
Marxfeld
Heike
H
Preclinical Safety, Novartis Pharma AG, Basel, Switzerland. heike.marxfeld@eur.crl.com
Grenet
Olivier
O
Bringel
Josiane
J
Staedtler
Frank
F
Harleman
Johannes H
JH
D015127:9,10-Dimethyl-1,2-benzanthracene; D000230:Adenocarcinoma / Q000235:genetics*; D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D015674:Mammary Neoplasms, Animal / Q000235:genetics* / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
D015127:9,10-Dimethyl-1,2-benzanthracene