Effect of fish or soybean oil-rich diets on bradykinin, kallikrein, nitric oxide, leptin, corticosterone and macrophages in carrageenan stimulated rats.
We have previously demonstrated that both n-3 and n-6 polyunsaturated fatty acids (PUFA)-rich diets decrease the acute inflammatory response partially explained by the high corticosterone basal levels. The present study aimed to determine the effect of hyperlipidic diets (PUFA n-3 or n-6) on phagocytosis, hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) release by macrophages, bradykinin (BK) and NO release in the paw inflammatory perfusate and Kallikrein (KK), corticosterone and leptin blood levels. Hyperlipidic diets decreased H(2)O(2) release from macrophages stimulated by carrageenan or phorbol-miristate-acetate (PMA), NO release from macrophage stimulated by carrageenan, BK and NO release in the edema perfusate, KK plasma levels and the increase of serum leptin after carrageenan stimulus. These data show that both fish and soybean oil-rich diets promote similar alterations on inflammatory mediators of carrageenan edema and a causal association with the anti-inflammatory effect of these diets.
Wohlers
Marta
M
Physiology Department, Federal University of São Paulo-EPM, Rua Botucatu 862-2 andar, Vila Clementino-CEP, São Paulo 04023-060, Brazil.
Xavier
Roberta Araujo Navarro
RA
Oyama
Lila Missae
LM
Ribeiro
Eliane Beraldi
EB
do Nascimento
Cláudia Maria Oller
CM
Casarini
Dulce Elena
DE
Silveira
Vera Lucia Flor
VL
D000818:Animals; D001920:Bradykinin / Q000096:biosynthesis* / Q000378:metabolism; D002351:Carrageenan / Q000494:pharmacology*; D003345:Corticosterone / Q000378:metabolism*; D005395:Fish Oils / Q000378:metabolism*; D005399:Fishes; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D007610:Kallikreins / Q000378:metabolism*; D020738:Leptin / Q000097:blood / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013024:Soybean Oil / Q000378:metabolism*
D005395:Fish Oils; D020738:Leptin; D009569:Nitric Oxide; D013024:Soybean Oil; D002351:Carrageenan; D006861:Hydrogen Peroxide; D007610:Kallikreins; D001920:Bradykinin; D003345:Corticosterone