title: Cell delivery in regenerative medicine: the cell sheet engineering approach.
abstract: Recently, cell-based therapies have developed as a foundation for regenerative medicine. General approaches for cell delivery have thus far involved the use of direct injection of single cell suspensions into the target tissues. Additionally, tissue engineering with the general paradigm of seeding cells into biodegradable scaffolds has also evolved as a method for the reconstruction of various tissues and organs. With success in clinical trials, regenerative therapies using these approaches have therefore garnered significant interest and attention. As a novel alternative, we have developed cell sheet engineering using temperature-responsive culture dishes, which allows for the non-invasive harvest of cultured cells as intact sheets along with their deposited extracellular matrix. Using this approach, cell sheets can be directly transplanted to host tissues without the use of scaffolding or carrier materials, or used to create in vitro tissue constructs via the layering of individual cell sheets. In addition to simple transplantation, cell sheet engineered constructs have also been applied for alternative therapies such as endoscopic transplantation, combinatorial tissue reconstruction, and polysurgery to overcome limitations of regenerative therapies and cell delivery using conventional approaches.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Joseph
authors.initials: J
authors.lastname: Yang
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Kohji
authors.initials: K
authors.lastname: Nishida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohki
authors.forename: Masato
authors.initials: M
authors.lastname: Kanzaki
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Sekine
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D021522:Bioartificial Organs; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004724:Endoscopy / Q000379:methods; D019573:Epithelium, Corneal / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D054018:Lasers, Excimer; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D018903:Photorefractive Keratectomy / Q000379:methods; D044968:Regenerative Medicine; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods; D014132:Trachea / Q000166:cytology / Q000637:transplantation
chemical_list: D000180:Acrylic Resins; C052970:poly-N-isopropylacrylamide