Adsorption of chlorhexidine and black tea onto in vitro salivary pellicles, as studied by ellipsometry.
The adsorption from 0.2% (w/w) chlorhexidine and black tea solutions onto an in vitro pellicle from whole unstimulated saliva on hydroxyapatite discs was studied by ellipsometry. It was found that chlorhexidine adsorbed to the pellicle and caused a modification of the pellicle properties, leading to a subsequent increase in adsorption of salivary and black tea components. There was a distinct order-of-addition effect, whereby chlorhexidine followed by black tea gave an overall greater adsorption of components compared with black tea followed by chlorhexidine. This increase in adsorption resulted in a concomitant increase in color or stain, as measured by a reflectance chromameter. The increase in adsorbed amounts and stain was modified, in part, by the adsorption of salivary fractions between the chlorhexidine and black tea treatments. In all cases, the chlorhexidine and black tea-modified pellicles were not readily removed by either phosphate or sodium dodecyl sulfate rinses. Thus, following exposure to chlorhexidine, the accelerated adsorption of salivary and black tea components can ultimately lead to increased staining of the pellicle.
Joiner
Andrew
A
Unilever Oral Care, Port Sunlight Laboratory, Wirral, Merseyside, UK. andrew.joiner@unilever.com
Elofsson
Ulla M
UM
Arnebrant
Thomas
T
D000327:Adsorption; D000891:Anti-Infective Agents, Local / Q000493:pharmacokinetics*; D002710:Chlorhexidine / Q000493:pharmacokinetics*; D003116:Color; D004396:Coloring Agents / Q000493:pharmacokinetics; D044622:Dental Pellicle / Q000378:metabolism*; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D055095:Optics and Photonics; D010710:Phosphates / Q000494:pharmacology; D012031:Refractometry; D012463:Saliva / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D013662:Tea / Q000378:metabolism*; D013997:Time Factors
D000891:Anti-Infective Agents, Local; D004396:Coloring Agents; D010710:Phosphates; D013501:Surface-Active Agents; D013662:Tea; D012967:Sodium Dodecyl Sulfate; D017886:Durapatite; D002710:Chlorhexidine; C018279:sodium phosphate