Full-length single-walled carbon nanotubes decorated with streptavidin-conjugated quantum dots as multivalent intracellular fluorescent nanoprobes.
We report the formation of a supramolecular luminescent nanoassembly composed of individual or small ropes of full-length, single-walled carbon nanotubes decorated with streptavidin-conjugated quantum dots. The supramolecular luminescent nanoassembly was stably dispersed under physiological conditions and was readily visible by both optical and confocal fluorescent microscopies. Jurkat T leukemia cells were able to internalize the nanoassembly by multivalent CD3 receptor-mediated endocytosis (adsorption by cell). Once internalized by cells, the nanoassembly was found to be transported to lysosomes. These properties should make this supramolecular luminescent nanoassembly an excellent building block for the construction of intracellular polyvalent nanoprobes, mimicking natural viral delivery entities with enhanced loading capacity compared to small molecules.
Bottini
Massimo
M
Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, California 92037, USA. mbottini@burnham.org
Cerignoli
Fabio
F
Dawson
Marcia I
MI
Magrini
Andrea
A
Rosato
Nicola
N
Mustelin
Tomas
T
D000327:Adsorption; D001692:Biological Transport; D017252:CD3 Complex / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods; D004705:Endocytosis; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D008247:Lysosomes / Q000378:metabolism; D015335:Molecular Probes / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry*; D045663:Quantum Dots; D019809:Streptavidin / Q000737:chemistry*; D014780:Viruses / Q000378:metabolism
D017252:CD3 Complex; D005456:Fluorescent Dyes; D015335:Molecular Probes; D037742:Nanotubes, Carbon; D019809:Streptavidin