Expression of single-chain Fv gene specific for gamma-seminoprotein by RTS and its biological activity identification.
Fabricating a single-chain variable fragment specific for human seminoprotein is very important in antibody-directed enzyme prodrug therapy and NMR imaging for prostate cancer. Here a single-chain Fv specific for gamma-seminoprotein was expressed by RTS. Its activity and the efficiency of entry into prostate cancer cells are investigated by immunoprecipitation and Western blotting and immunofluorescent staining, as well as entry of conjugated magnetic beads into cells. Results showed that ScFv peptides specific for gamma-seminoprotein were successfully prepared, which can bind with the prostate cells specifically and can bring magnetic beads into prostate cancer cells within 15 min, the amount of magnetic beads inside prostate cancer cells increased as the culture time prolonged. ScFv-conjugated magnetic beads did not enter into control cells. In conclusion, the ScFv peptide against human gamma-seminoprotein with biological activity was successfully fabricated, which can take magnetic beads to prostate cancer cells specifically and not to the control cells. This ScFv peptide against human gamma-seminoprotein should be useful in improving the detection and therapy of prostate cancer at early stages and NMR imaging.
Han
Yuedong
Y
Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, PR China. yuedonghan@162.com
Haun
Yi
Y
Deng
Jinlan
J
Gao
Feng
F
Pan
Bifeng
B
Cui
Daxiang
D
D001665:Binding Sites; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002474:Cell-Free System; D005455:Fluorescent Antibody Technique; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D018189:Immunomagnetic Separation; D008280:Magnetics; D008297:Male; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000737:chemistry / Q000276:immunology*; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000378:metabolism; D014176:Protein Biosynthesis; D012680:Sensitivity and Specificity
D007128:Immunoglobulin Fragments; C055966:immunoglobulin Fv; D017430:Prostate-Specific Antigen