title: Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice.
abstract: Most Oryza sativa cultivars die within a week of complete submergence--a major constraint to rice production in south and southeast Asia that causes annual losses of over US 1 billion dollars and affects disproportionately the poorest farmers in the world. A few cultivars, such as the O. sativa ssp. indica cultivar FR13A, are highly tolerant and survive up to two weeks of complete submergence owing to a major quantitative trait locus designated Submergence 1 (Sub1) near the centromere of chromosome 9 (refs 3, 4, 5-6). Here we describe the identification of a cluster of three genes at the Sub1 locus, encoding putative ethylene response factors. Two of these genes, Sub1B and Sub1C, are invariably present in the Sub1 region of all rice accessions analysed. In contrast, the presence of Sub1A is variable. A survey identified two alleles within those indica varieties that possess this gene: a tolerance-specific allele named Sub1A-1 and an intolerance-specific allele named Sub1A-2. Overexpression of Sub1A-1 in a submergence-intolerant O. sativa ssp. japonica conferred enhanced tolerance to the plants, downregulation of Sub1C and upregulation of Alcohol dehydrogenase 1 (Adh1), indicating that Sub1A-1 is a primary determinant of submergence tolerance. The FR13A Sub1 locus was introgressed into a widely grown Asian rice cultivar using marker-assisted selection. The new variety maintains the high yield and other agronomic properties of the recurrent parent and is tolerant to submergence. Cultivation of this variety is expected to provide protection against damaging floods and increase crop security for farmers.
authors.affiliation: Department of Plant Pathology, University of California, Davis, California 95616, USA.
authors.forename: Kenong
authors.initials: K
authors.lastname: Xu
authors.forename: Xia
authors.initials: X
authors.lastname: Xu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fukao
authors.forename: Patrick
authors.initials: P
authors.lastname: Canlas
authors.forename: Reycel
authors.initials: R
authors.lastname: Maghirang-Rodriguez
authors.forename: Sigrid
authors.initials: S
authors.lastname: Heuer
authors.forename: Abdelbagi M
authors.initials: AM
authors.lastname: Ismail
authors.forename: Julia
authors.initials: J
authors.lastname: Bailey-Serres
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald
authors.forename: David J
authors.initials: DJ
authors.lastname: Mackill
mesh_terms: D000483:Alleles; D032461:Chromosomes, Plant / Q000235:genetics; D018556:Crops, Agricultural / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D005030:Ethylenes / Q000494:pharmacology*; D017343:Genes, Plant / Q000235:genetics*; D006239:Haplotypes; D008969:Molecular Sequence Data; D012275:Oryza / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D014867:Water / Q000494:pharmacology*
chemical_list: D005030:Ethylenes; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D014867:Water; C036216:ethylene