Poor recognition of HIV-1 Nef protein by CD8 T cells from HIV-1-infected children: impact of age.
Recognition of various HIV proteins by CD8 T cells from HIV-infected children was determined by two functional assays. First, using an Elispot assay, we show that 80% of patients recognized Gag, 77% recognized Pol, 61% recognized Env, 44% recognized Nef and 29% recognized Vif. Frequencies of Gag-, Pol-, and Env-specific IFN-gamma producing CD8 T cells were higher than frequencies of Nef and Vif-specific CD8 T cells. The poor recognition of Nef by ex vivo CD8 T cells was confirmed by CTL assays performed in HAART naïve children: 25% of children had positive response against Nef versus 44, 63 and 62% for Env, Gag, and Pol, respectively. Memory Gag-specific CTL were positively correlated with age, whereas Nef-specific CTL were negatively correlated with age. The poor Nef-specific CD8 T cell response in HIV-infected children contrasts with dominance of Nef-specific responses in infected adults.
Buseyne
Florence
F
Unité Postulante d'Immunopathologie Virale, URA CNRS 1930, Institut Pasteur, Bat Lwoff, 28 rue du Dr Roux, 75015 Paris, France. florence@pasteur.fr
Scott-Algara
Daniel
D
Corre
Béatrice
B
Porrot
Françoise
F
Monchatre
Elizabeth
E
Bellal
Nassima
N
Burgard
Marianne
M
Rouzioux
Christine
C
Blanche
Stéphane
S
Rivière
Yves
Y
D000367:Age Factors; D019380:Anti-HIV Agents / Q000627:therapeutic use; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002675:Child, Preschool; D015693:Gene Products, nef / Q000276:immunology / Q000378:metabolism*; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007223:Infant; D012189:Retrospective Studies; D054311:nef Gene Products, Human Immunodeficiency Virus
D019380:Anti-HIV Agents; D015693:Gene Products, nef; D054311:nef Gene Products, Human Immunodeficiency Virus