title: [Cytoprotective effects of heat shock protein-70 in bronchial epithelium against neutrophil elastase-induced cell injury].
abstract: BACKGROUND
  Neutrophil releases several mediators during inflammation, including neutrophil elastase (NE) that impairs bronchial epithelial function. The stress response and stress proteins protect cells against a variety of cytotoxic conditions. Accordingly, we tested the hypothesis that bronchial epithelial heat shock protein (Hsp-70) would protect a NE-induced cell injury.
  
  
  METHODS
  Bronchial epithelial cells (BECs) obtained by bronchial brushing under bronchoscopy were cultured and used for experiments. Expression of Hsp-70 in BECs was confirmed by Western blot and flowcytometric analysis. To test Hsp-70 in BECs, induction of Hsp-70 protein into BECs was carried out by liposome-based delivery system. Introduction of Hsp-70 into BECs were examined by direct fluorescence microscope examination and flowcytometric analysis. NE-induced cytotoxicity was evaluated by cell culture supernatant LDH assay and cell detachment assay.
  
  
  RESULTS
  Higher expressions of Hsp-70 were observed in BECs, which were induced by sodium arsenite. Over expression of Hsp-70 in BECs reduced NE-induced cell injury. Introduction of Hsp-70 protein into BECs by liposomal delivery decreased LDH release, and inhibited necrosis and apoptosis of the cells by NE as compared to untreated control.
  
  
  CONCLUSIONS
  These data suggested that Hsp-70 in BECs may inhibit NE-induced airway epithelial damage. Liposomal delivery of Hsp-70 into BECs may be a possible means of protecting bronchial epithelium against inflammatory airway diseases including acute and chronic bronchitis.
authors.affiliation: Internal Medicine III, Iwate Medical University.
authors.forename: Harumasa
authors.initials: H
authors.lastname: Ito
authors.forename: Kenshi
authors.initials: K
authors.lastname: Sekimura
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Sasaki
authors.forename: Hiroo
authors.initials: H
authors.lastname: Nitanai
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Toshiki
authors.initials: T
authors.lastname: Shikanai
authors.forename: Takashi
authors.initials: T
authors.lastname: Mouri
authors.forename: Kohei
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Inoue
mesh_terms: D001980:Bronchi / Q000737:chemistry / Q000502:physiology*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000737:chemistry / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000502:physiology*; D006801:Humans; D019272:Leukocyte Elastase / Q000502:physiology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D019272:Leukocyte Elastase