Production of reactive oxygen species in peripheral blood is increased in individuals with Helicobacter pylori infection and decreased after its eradication.
BACKGROUND
Helicobacter pylori infection has been reported to cause gastroduodenal ulcer, mucosa-associated lymphoid tissue lymphoma, and gastric cancer. Recent studies have suggested that H. pylori infection may also associate with other diseases, including hematologic and dermatologic disorders, and cardiovascular injury, by unknown mechanisms.


METHODS
Production of reactive oxygen species (ROS) was determined in peripheral blood samples from 86 patients (34 H. pylori-negative and 52 H. pylori-positive subjects) using a highly sensitive chemiluminescence probe, L-012 (8-amino-5-chloro-7-phenylpyrido(3,4-d) pyridazine-1 and 4 (2H, 3H) dione). Eleven H. pylori-positive individuals were also analyzed their ROS production in peripheral blood after H. pylori eradication.


RESULTS
ROS production was significantly higher in individuals with H. pylori infection than in those without infection. Enhanced production of ROS was decreased significantly after eradication of H. pylori. No correlation was found between the extent of ROS production and sex, age, smoking status, alcohol ingestion, use of medications, or serum level of C-reactive protein.


CONCLUSIONS
These findings suggest that ROS production was enhanced in peripheral blood by H. pylori infection. Chemiluminescence analysis of blood samples using L-012 permits evaluation of systemic oxidative stress in patients with H. pylori infection.
Mashimo
Masayuki
M
Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, Osaka 545-8585, Japan.
Nishikawa
Manabu
M
Higuchi
Kazuhide
K
Hirose
Masaki
M
Wei
Qian
Q
Haque
Anwarul
A
Sasaki
Eiji
E
Shiba
Masatsugu
M
Tominaga
Kazunari
K
Watanabe
Toshio
T
Fujiwara
Yasuhiro
Y
Arakawa
Tetsuo
T
Inoue
Masayasu
M
D000328:Adult; D000367:Age Factors; D000368:Aged; D000428:Alcohol Drinking; D002097:C-Reactive Protein / Q000032:analysis; D016022:Case-Control Studies; D005260:Female; D016481:Helicobacter Infections / Q000097:blood* / Q000188:drug therapy / Q000473:pathology; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000097:blood*; D012737:Sex Factors; D012907:Smoking
D017382:Reactive Oxygen Species; D002097:C-Reactive Protein