Protection by rhamnose-rich polysaccharides against the cytotoxicity of Maillard reaction products.
Previous experiments have shown that AGE-products added to human skin fibroblast cultures increased the number of dead cells floating on top of the culture fluid and took up vital dye [1]. In these experiments, we tested several rhamnose-rich polysaccharides for protection against the cytotoxic effect of AGE-s. Added at relatively low concentrations (between 10 and 100 microg/ml) to the culture medium, several of the tested rhamnose-rich oligo- and polysaccharides (RROP-s) gave a significant protection against AGE-induced cytotoxicity. Their effect on cell proliferation was also tested. The number of cells at saturation density was also shown to be influenced by AGE-products added to the cultures. This effect was also, at least partially, corrected by the rhamnose-rich oligo- and polysaccharides. These substances might therefore be considered as of potential therapeutical interest against hyperglycemia induced cytotoxic effects as in type II-diabetes.
Ravelojaona
V
V
Laboratoire de recherche ophtalmique, Hôtel-Dieu, Université Paris-V, 1, place Parvis-Notre-Dame, 75181 Paris cedex 04, France.
Molinari
J
J
Robert
L
L
D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D006801:Humans; D015416:Maillard Reaction; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D012210:Rhamnose / Q000737:chemistry / Q000494:pharmacology*
D017127:Glycation End Products, Advanced; D009844:Oligosaccharides; D011134:Polysaccharides; D012210:Rhamnose