title: Expression and GTP sensitivity of peptide histidine isoleucine high-affinity-binding sites in rat.
abstract: High-affinity-binding sites for the vasoactive intestinal peptide (VIP) analogs peptide histidine/isoleucine-amide (PHI)/carboxyterminal methionine instead of isoleucine (PHM) are expressed in numerous tissues in the body but the nature of their receptors remains to be elucidated. The data presented indicate that PHI discriminated a high-affinity guanosine 5'-triphosphate (GTP)-insensitive-binding subtype that represented the totality of the PHI-binding sites in newborn rat tissues but was differentially expressed in adult animals. The GTP-insensitive PHI/PHM-binding sites were also observed in CHO cells over expressing the VPAC2 but not the VPAC1 VIP receptor.
authors.affiliation: IPBC CNRS-UMR 6187 Pôle Biologie Santé, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
authors.forename: Colin
authors.initials: C
authors.lastname: Debaigt
authors.forename: Annie-Claire
authors.initials: AC
authors.lastname: Meunier
authors.forename: Stephanie
authors.initials: S
authors.lastname: Goursaud
authors.forename: Alicia
authors.initials: A
authors.lastname: Montoni
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pineau
authors.forename: Alain
authors.initials: A
authors.lastname: Couvineau
authors.forename: Marc
authors.initials: M
authors.lastname: Laburthe
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Muller
authors.forename: Thierry
authors.initials: T
authors.lastname: Janet
mesh_terms: D000818:Animals; D001665:Binding Sites; D016466:CHO Cells; D006224:Cricetinae; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D010451:Peptide PHI / Q000378:metabolism* / Q000494:pharmacology*; D051381:Rats; D051239:Receptors, Vasoactive Intestinal Peptide, Type II / Q000235:genetics / Q000378:metabolism; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D010451:Peptide PHI; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; D011994:Recombinant Proteins; D006160:Guanosine Triphosphate