Massively parallel signature sequencing.
Massively parallel signature sequencing is an ultra-high throughput sequencing technology. It can simultaneously sequence millions of sequence tags, and, therefore, is ideal for whole genome analysis. When applied to expression profiling, it reveals almost every transcript in the sample and provides its accurate expression level. This chapter describes the technology and its application in establishing stem cell transcriptome databases.
Zhou
Daixing
D
Lynx Therapeutics Inc., Hayward, CA, USA.
Rao
Mahendra S
MS
Walker
Roger
R
Khrebtukova
Irina
I
Haudenschild
Christian D
CD
Miura
Takumi
T
Decola
Shannon
S
Vermaas
Eric
E
Moon
Keith
K
Vasicek
Thomas J
TJ
D018929:Cell Culture Techniques / Q000379:methods; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D017422:Sequence Analysis, DNA / Q000379:methods; D014158:Transcription, Genetic