MR tracking of transplanted glial cells using poly-L-lysine-CF3.
Magnetic resonance (MR) imaging using super-paramagnetic iron oxides (SPIOs) is a powerful tool to monitor transplanted cells in living animals. Since, however, SPIOs are negative contrast agents, positive agents have been explored. In this study, we examined the feasibility of FITC-labeled poly-L-lysine-CF3 (PLK-CF3) using glial cells. FITC-labeled PLK-CF3 was easily internalized by neuroblastoma cells and glia as adding it into culture medium. No toxicity was seen at the concentration of less than 80 microg/ml. MR images positively detected labeled cells transplanted in the brain of living mouse. The results indicate that FITC-labeled PLK-CF3 is a useful positive contrast agent for MR tracking.
Masuda
Chiaki
C
Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu 520-2192, Japan.
Maki
Zyunichi
Z
Morikawa
Shigehiro
S
Morita
Masahito
M
Inubushi
Toshiro
T
Matsusue
Yoshitaka
Y
Yamagata
Sonoko
S
Taguchi
Hiroyasu
H
Doi
Yukio
Y
Shirai
Nobuaki
N
Hirao
Koichi
K
Tooyama
Ikuo
I
D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D003287:Contrast Media; D052203:Ferrosoferric Oxide / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009457:Neuroglia / Q000502:physiology / Q000637:transplantation*; D011107:Polylysine / Q000378:metabolism*; D051381:Rats
D003287:Contrast Media; D011107:Polylysine; D052203:Ferrosoferric Oxide