Purification and characterization of Ophiophagus hannah cytotoxin-like proteins.
Three cytotoxin-like proteins from the venom of Ophiophagus hannah were isolated by a combination of ion exchange chromatography and reverse phase HPLC. Amino acid sequence analysis revealed that these proteins all consisted of 63 amino acids and shared approximate 50% and 56% sequence identity with Naja naja atra cardiotoxins and cardiotoxin-like basic proteins (CLBPs), respectively. CD spectra revealed that their secondary structure was dominated with beta-sheet as those noted with cardiotoxins and CLBPs. O. hannah cytotoxin-like protein exhibited a cell-lytic activity on SK-N-SH cells, but its activity was more weak than that noted for N. naja atra cardiotoxin 3. Alternatively, apoptotic cell death was induced by the addition of N. naja atra CLBP. Based on the sequence information with the toxin molecules, the functional residues and regions related to the differential activity with O. hannah cytotoxin-like protein, cardiotoxin and CLBP are discussed.
Chang
Long-Sen
LS
Institute of Biomedical Sciences, National Sun Yat-Sen Unversity-Kaohsiung Medical University Joint Center, National Sun Yat-Sen University, Kaohsiung 804, Taiwan, ROC. lschang@mail.nsysu.edu.tw
Chen
Ku-Chung
KC
Lin
Shinne-Ren
SR
Huang
Hsien-Bin
HB
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D004179:Cobra Cardiotoxin Proteins / Q000302:isolation & purification; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D004546:Elapid Venoms / Q000032:analysis*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011506:Proteins / Q000302:isolation & purification*
D004179:Cobra Cardiotoxin Proteins; D003603:Cytotoxins; D004546:Elapid Venoms; D011506:Proteins