Bioavailability of salvianolic acid B in conscious and freely moving rats.
Salvianolic acid B is an herbal ingredient isolated from Salvia miltiorrhiza. The aim of this study was to apply an automated blood sampling system coupled to a simple liquid chromatographic system to determine the bioavailability of salvianolic acid B in stress-free rats. The plasma sample (25 microl) was vortex-mixed with 50 microl of internal standard solution (chloramphenicol 10 microg/ml in acetonitrile) to achieve protein precipitation. Salvianolic acid B in the rat plasma was separated using a reversed-phase C18 column (250 mm x 4.6 mm, 5 microm) with a mobile phase of acetonitrile-methanol-20mM NaH(2)PO(4) (adjusted to pH 3.5 with H(3)PO(4)) (20:10:70 v/v/v) containing 0.1mM 1-octanesulfonic acid, and the flow-rate of 1 ml/min. The UV detection wavelength was 286 nm. The concentration-response relationship from the present method indicated linearity over a concentration range of 0.5-200 microg/ml. Intra- and inter-assay precision and accuracy of salvianolic acid B fell well within the predefined limits of acceptability (<15%). The plasma sample of salvianolic acid B was further identified by LC-MS/MS in the negative ion mode using mass transition m/z 358.2 to the product ion m/z 196.9. After salvianolic acid B (100mg/kg, i.v.; 500 mg/kg, p.o.) was given in conscious and freely moving rats, the AUC were 5030+/-565 and 582+/-222 min microg/ml for intravenous (100 mg/kg) and oral (500 mg/kg) doses, respectively. The oral bioavailability of salvianolic acid B in freely moving rats was calculated to be 2.3%.
Wu
Yu-Tse
YT
Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, 155, Li-Nong Street Section 2, Taipei 112, Taiwan.
Chen
Yen-Fei
YF
Hsieh
Yen-Ju
YJ
Jaw
Ine
I
Shiao
Ming-Shi
MS
Tsai
Tung-Hu
TH
D000818:Animals; D001331:Automation; D001572:Benzofurans / Q000493:pharmacokinetics*; D002626:Chemistry, Pharmaceutical / Q000295:instrumentation* / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D007477:Ions; D013058:Mass Spectrometry; D008956:Models, Chemical; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000378:metabolism; D014466:Ultraviolet Rays; D053630:Depsides
D001572:Benzofurans; D007477:Ions; C076944:salvianolic acid B; D053630:Depsides