Influence of mechanical stress on cell viability.
The cartilage is a hydrated connective tissue in joints that withstands and distributes mechanical forces. The chondrocytes utilize mechanical signals to regulate their metabolic activity through complex biological and biophysical interactions with the extracellular matrix (ECM). The aim of this work was to study the influence of mechanical stress on cells behavior cultured in 3D biosystems (alginate and alginate supplemented with hyaluronate). After mechanical stimulation, cell viability and cell death process were the main studied parameters. Our results indicated that viability and cell cycle progression were inhibited under mechanical stimulation, as far as the extracellular matrix was not yet synthesized. In contrast, on day 21, the mechanical stimulation had positive effect on these parameters.
Huselstein
C
C
Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA UMR INPL CNRS 7563 et IFR 111 Bioingénierie, Faculté de Médecine, 54505 Vandoeuvre-lès-Nancy Cedex, France. Celine.Huselstein@medecine.uhp-nancy.fr
de Isla
N
N
Kolopp-Sarda
M N
MN
Kerdjoudj
H
H
Muller
S
S
Stoltz
J F
JF
D000464:Alginates; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002358:Cartilage, Articular / Q000166:cytology* / Q000502:physiology; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology; D016923:Cell Death / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology*; D005109:Extracellular Matrix; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid