Determination and validation of ketoprofen, pantoprazole and valsartan together in human plasma by high performance liquid chromatography.
A rapid and specific high-performance liquid chromatographic method was developed and validated for the simultaneous determination of ketoprofen, valsartan and pantoprazole in human plasma. Chromatographic separation of ketoprofen, valsartan and pantoprazole was performed using a Chromasil C18 column (250 mm x 4.6 mm i.d., 5 microm particle size). The mobile phase consisted of a mixture of 0.02 M sodium dihydrogen phosphate buffer (pH 3.15) and acetonitrile (58:42, v/v) pumped through the chromatographic system at a flow rate of 1 mL x min(-1). The Diode Array detector was operated at 225 and 272 nm. Rofecoxib was used as an internal standard. Sample treatment procedure consisted of deproteinisation with acetonitrile-methanol (50:50 v/v). Analytical recoveries were in the range of 79.00-118.00% of nominal values of valsartan, ketoprofen and pantoprazole. The method was reproducible and accurate with lower limits of quantification 250 microg x L(-1) for pantoprazole and 500 microg x L(-1) for ketoprofen and valsartan. This method was relatively easy to perform and allows simultaneous determination of these three drugs in plasma at nanogram levels.
Koçyiğit-Kaymakçoğlu
B
B
Department of Pharmaceutical Chemistry, Facult of Pharmacy, Marmara University, Istanbul, Turkey.
Unsalan
S
S
Rollas
S
S
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D000897:Anti-Ulcer Agents / Q000097:blood*; D000959:Antihypertensive Agents / Q000097:blood*; D001562:Benzimidazoles / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007660:Ketoprofen / Q000097:blood*; D009853:Omeprazole / Q000031:analogs & derivatives* / Q000097:blood; D000077402:Pantoprazole; D012015:Reference Standards; D015203:Reproducibility of Results; D013454:Sulfoxides / Q000097:blood*; D013777:Tetrazoles / Q000097:blood*; D014633:Valine / Q000031:analogs & derivatives* / Q000097:blood; D000068756:Valsartan
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D000959:Antihypertensive Agents; D001562:Benzimidazoles; D007202:Indicators and Reagents; D013454:Sulfoxides; D013777:Tetrazoles; D000068756:Valsartan; D007660:Ketoprofen; D000077402:Pantoprazole; D014633:Valine; D009853:Omeprazole