Anti-stress activity of mulberry juice in mice.
The possible anti-stress activity of mulberry juice was investigated in mice. When mice were subjected to water immersion restraint stress at 25 degrees C for 8 h, the plasma lipid peroxide level, determined by the d-ROMs test performed 12 h thereafter, was almost doubled. After administration of mulberry juice one or two weeks before the stress loading, the lipid peroxidation was completely blocked. Administration of mulberry juice after the stress loading, without pre-administration, was also protective. ESR spectroscopy revealed that mulberry juice scavenged superoxide anion (generated by hypoxanthine and xanthine oxidase reaction), hydroxyl radical (produced by the Fenton reaction) and NO radical (generated by a NO donor) at approximately 50% efficiency of blueberry juice. Mulberry juice produced smaller amounts of radical at neutral to alkaline pH. The cytotoxic and anti-HIV activities of mulberry juice were 18% and >4-fold those of blueberry juice, respectively. These data suggest that the anti-stress activity of mulberry juice in vivo may be derived from its radical scavenging activity.
Sakagami
Hiroshi
H
Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama, Japan. sakagami@dent.meikai.ac.jp
Asano
Kazuhito
K
Satoh
Kazue
K
Takahashi
Keiso
K
Terakubo
Shigemi
S
Shoji
Yoko
Y
Nakashima
Hideki
H
Nakamura
Wataru
W
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism; D005881:Gingiva / Q000166:cytology; D006801:Humans; D007101:Immersion / Q000009:adverse effects; D007938:Leukemia / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D031625:Morus / Q000737:chemistry*; D009062:Mouth Neoplasms / Q000188:drug therapy; D010513:Periodontal Ligament / Q000166:cytology; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013315:Stress, Psychological / Q000188:drug therapy*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D016166:Free Radical Scavengers; D010936:Plant Extracts