Usefulness of Toxoplasma gondii recombinant antigens (GRA1, GRA7 and SAG1) in an immunoglobulin G avidity test for the serodiagnosis of toxoplasmosis.
The precise diagnosis of an acute and recent Toxoplasma infection in pregnant women and the newborn child is important before treatment. This study describes a new Toxoplasma gondii IgG avidity test based on a combination of recombinant GRA1, GRA7 and SAG1 antigens and shows that this test is useful for diagnostic purposes and may replace the lysed, whole-cell antigens. Although more sera need to be tested, the results obtained here suggest that the IgG avidity test performed with rec-antigens correlated more with the stage of a T. gondii infection than the IgG avidity results obtained with the lysed, whole-cell antigen test, the VIDAS Toxo IgG avidity (bioMérieux).
Pietkiewicz
H
H
Department of Tropical Parasitology, Inter-Faculty Institute of Maritime and Tropical Medicine, Medical University of Gdańsk, ul. Powstania Styczniowego 9b, Gdynia, Poland.
Hiszczyńska-Sawicka
E
E
Kur
J
J
Petersen
E
E
Nielsen
H V
HV
Paul
M
M
Stankiewicz
M
M
Myjak
P
P
D000818:Animals; D000915:Antibody Affinity; D000953:Antigens, Protozoan / Q000276:immunology*; D007074:Immunoglobulin G; D015800:Protozoan Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D014122:Toxoplasma / Q000378:metabolism*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology
D000953:Antigens, Protozoan; C412913:GRA1 antigen; C112573:GRA7 protein, Toxoplasma gondii; D007074:Immunoglobulin G; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma