Mosla dianthera inhibits mast cell-mediated allergic reactions through the inhibition of histamine release and inflammatory cytokine production.
In this study, we investigated the effect of the aqueous extract of Mosla dianthera (Maxim.) (AEMD) on the mast cell-mediated allergy model and studied the possible mechanism of action. Mast cell-mediated allergic disease is involved in many diseases such as asthma, sinusitis and rheumatoid arthritis. The discovery of drugs for the treatment of allergic disease is an important subject in human health. AEMD inhibited compound 48/80-induced systemic reactions in mice. AEMD decreased immunoglobulin E-mediated local allergic reactions, passive cutaneous anaphylaxis. AEMD attenuated intracellular calcium level and release of histamine from rat peritoneal mast cells activated by compound 48/80. Furthermore, AEMD attenuated the phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-stimulated TNF-alpha, IL-8 and IL-6 secretion in human mast cells. The inhibitory effect of AEMD on the pro-inflammatory cytokines was nuclear factor-kappaB (NF-kappaB) dependent. AEMD decreased PMA and A23187-induced degradation of IkappaBalpha and nuclear translocation of NF-kappaB. Our findings provide evidence that AEMD inhibits mast cell-derived immediate-type allergic reactions and involvement of pro-inflammatory cytokines and NF-kappaB in these effects.
Lee
Dong-Hee
DH
College of Pharmacy, Woosuk University, Jeonju 565-701, Republic of Korea.
Kim
Sang-Hyun
SH
Eun
Jae-Soon
JS
Shin
Tae-Yong
TY
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D024202:Electrophoretic Mobility Shift Assay; D006633:Histamine Antagonists; D006636:Histamine Release / Q000187:drug effects*; D006967:Hypersensitivity / Q000188:drug therapy*; D007073:Immunoglobulin E / Q000502:physiology; D007249:Inflammation / Q000378:metabolism*; D015850:Interleukin-6 / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D009687:Nuclear Proteins / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection; D014197:Trees / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D016207:Cytokines; D006633:Histamine Antagonists; D015850:Interleukin-6; D016209:Interleukin-8; D009687:Nuclear Proteins; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D008156:Luciferases; D002118:Calcium