title: A porcine deep dermal partial thickness burn model with hypertrophic scarring.
abstract: We developed a reproducible model of deep dermal partial thickness burn injury in juvenile Large White pigs. The contact burn is created using water at 92 degrees C for 15s in a bottle with the bottom replaced with plastic wrap. The depth of injury was determined by a histopathologist who examined tissue sections 2 and 6 days after injury in a blinded manner. Upon creation, the circular wound area developed white eschar and a hyperaemic zone around the wound border. Animals were kept for 6 weeks or 99 days to examine the wound healing process. The wounds took between 3 and 5 weeks for complete re-epithelialisation. Most wounds developed contracted, purple, hypertrophic scars. On measurement, the thickness of the burned skin was approximately 1.8 times that of the control skin at week 6 and approximately 2.2 times thicker than control skin at 99 days after injury. We have developed various methods to assess healing wounds, including digital photographic analysis, depth of organising granulation tissue, immunohistochemistry, electron microscopy and tensiometry. Immunohistochemistry and electron microscopy showed that our porcine hypertrophic scar appears similar to human hypertrophic scarring. The development of this model allows us to test and compare different treatments on burn wounds.
authors.affiliation: Royal Children's Hospital Burns Research Group, University of Queensland, Department of Paediatrics and Child Health, Royal Children's Hospital, Herston, Queensland 4029, Australia. L.Cuttle@uq.edu.au
authors.forename: Leila
authors.initials: L
authors.lastname: Cuttle
authors.forename: Margit
authors.initials: M
authors.lastname: Kempf
authors.forename: Gael E
authors.initials: GE
authors.lastname: Phillips
authors.forename: Julie
authors.initials: J
authors.lastname: Mill
authors.forename: Mark T
authors.initials: MT
authors.lastname: Hayes
authors.forename: John F
authors.initials: JF
authors.lastname: Fraser
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Wang
authors.forename: Roy M
authors.initials: RM
authors.lastname: Kimble
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology*; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D003094:Collagen / Q000648:ultrastructure; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D012867:Skin / Q000293:injuries* / Q000648:ultrastructure; D013552:Swine
chemical_list: D003094:Collagen