title: G protein-coupled receptor kinase 2 and beta-arrestins are recruited to FSH receptor in stimulated rat primary Sertoli cells.
abstract: FSH-receptor (FSH-R) signaling is regulated by agonist-induced desensitization and internalization. It has been shown, in a variety of overexpression systems, that G protein-coupled receptor kinases (GRKs) phosphorylate the activated FSH-R, promote beta-arrestin recruitment and ultimately lead to internalization. The accuracy of this mechanism has not yet been demonstrated in cells expressing these different molecules at physiological levels. Using sucrose gradient fractionation, we show that FSH induces the recruitment of the endogenous GRK 2 and beta-arrestin 1/2 from the cytoplasm to the plasma membrane of rat primary Sertoli cells. As assessed by ligand binding, the FSH-R was found expressed in the fractions where GRK 2 and beta-arrestins were recruited upon FSH treatment. In addition, the endogenous beta-arrestin 1 was found dephosphorylated in an agonist-dependent manner. Finally, a significant FSH-binding activity was co-immunoprecipitated with the endogenous beta-arrestins from agonist-stimulated but not from untreated Sertoli cell extracts. This FSH-R interaction with beta-arrestins was sustained for up to 30 min. In conclusion, our data strongly suggest that the GRK/beta-arrestin machinery plays a physiologically relevant role in the regulation of the FSH signaling.
authors.affiliation: UMR 6175, INRA/CNRS/Université de Tours, Station de Physiologie de la Reproduction et des Comportements, 37380 Nouzilly, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Marion
authors.forename: Elodie
authors.initials: E
authors.lastname: Kara
authors.forename: Pascale
authors.initials: P
authors.lastname: Crepieux
authors.forename: Vincent
authors.initials: V
authors.lastname: Piketty
authors.forename: Nadine
authors.initials: N
authors.lastname: Martinat
authors.forename: Florian
authors.initials: F
authors.lastname: Guillou
authors.forename: Eric
authors.initials: E
authors.lastname: Reiter
mesh_terms: D000818:Animals; D019390:Arrestins / Q000378:metabolism*; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015151:Immunoblotting / Q000379:methods; D007124:Immunoenzyme Techniques; D047468:Immunoprecipitation; D008297:Male; D010766:Phosphorylation; D011480:Protease Inhibitors / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D011962:Receptors, FSH / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013268:Stimulation, Chemical; D000071559:beta-Arrestin 1; D000071557:beta-Arrestins
chemical_list: C000605256:Arrb1 protein, rat; D019390:Arrestins; D011480:Protease Inhibitors; D011962:Receptors, FSH; D043562:Receptors, G-Protein-Coupled; D000071559:beta-Arrestin 1; D000071557:beta-Arrestins; D005640:Follicle Stimulating Hormone