title: Methodological approach to minimal residual disease detection by flow cytometry in adult B-lineage acute lymphoblastic leukemia.
abstract: A flow cytometric approach to minimal residual disease (MRD) monitoring useful in childhood B-lineage acute lymphoblastic leukemia (ALL) is discussed here in the context of ALL in adults. Of 64 leukemia samples analyzed, 95.3% had at least one abnormal phenotype (57.3% had two or more) as compared to physiologic B-cell precursors in adult bone marrow. The method was sensitive enough to detect one leukemic cell among 10,000 normal cells in 16/19 experiments (84.2%). Blast phenotypes were stable in culture and at relapse, and were useful for MRD monitoring in patients. Marker combinations for childhood ALL are also applicable to adult cases.
authors.affiliation: Dipartimento di Medicina Clinica e Sperimentale, Sezione di Ematologia, Università di Verona. mauro.krampera@univr.it
authors.forename: Mauro
authors.initials: M
authors.lastname: Krampera
authors.forename: Omar
authors.initials: O
authors.lastname: Perbellini
authors.forename: Carlo
authors.initials: C
authors.lastname: Vincenzi
authors.forename: Francesca
authors.initials: F
authors.lastname: Zampieri
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pasini
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Scupoli
authors.forename: Anna
authors.initials: A
authors.lastname: Guarini
authors.forename: Maria Stefania
authors.initials: MS
authors.lastname: De Propris
authors.forename: Elaine
authors.initials: E
authors.lastname: Coustan-Smith
authors.forename: Dario
authors.initials: D
authors.lastname: Campana
authors.forename: Robin
authors.initials: R
authors.lastname: Foà
authors.forename: Giovanni
authors.initials: G
authors.lastname: Pizzolo
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000473:pathology; D001854:Bone Marrow Cells / Q000166:cytology / Q000473:pathology; D002051:Burkitt Lymphoma / Q000473:pathology*; D002648:Child; D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D018365:Neoplasm, Residual / Q000473:pathology*; D012016:Reference Values; D012038:Regeneration
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016650:Fluorescein-5-isothiocyanate