title: Nanoparticle-based sensing of glycan-lectin interactions.
abstract: Here we present the first report on nanoparticle-based biosensing of glycan markers of diseases. The protocol relies on the competition between a nanocrystal (CdS)-tagged sugar and the target sugar for the binding sites of surface-confined lectin and monitoring the extent of competition through highly sensitive electrochemical detection of the captured nanocrystal. This development is expected to allow decentralized detection of carbohydrate moieties and lectin-carbohydrate interactions to be performed more rapidly, sensitively, inexpensively, and reliably.
authors.affiliation: The Biodesign Institute and Fulton School of Engineering, Arizona State University, Tempe, Arizona 85287-5801, USA.
authors.forename: Zong
authors.initials: Z
authors.lastname: Dai
authors.forename: Abdel-Nasser
authors.initials: AN
authors.lastname: Kawde
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: La Belle
authors.forename: Jared
authors.initials: J
authors.lastname: Gerlach
authors.forename: Veer P
authors.initials: VP
authors.lastname: Bhavanandan
authors.forename: Lokesh
authors.initials: L
authors.lastname: Joshi
authors.forename: Joseph
authors.initials: J
authors.lastname: Wang
mesh_terms: D037102:Lectins / Q000737:chemistry*; D053758:Nanoparticles; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D037102:Lectins; D011134:Polysaccharides