Nanoscale resolution in GFP-based microscopy.
We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.
Willig
Katrin I
KI
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
Kellner
Robert R
RR
Medda
Rebecca
R
Hein
Birka
B
Jakobs
Stefan
S
Hell
Stefan W
SW
D000818:Animals; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D021621:Imaging, Three-Dimensional; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000451:nursing*; D008870:Microtubules / Q000648:ultrastructure*; D036103:Nanotechnology; D025682:Nanovirus; D021381:Protein Transport; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins