[A study on the expression of HSP70 in endothelial cells pretreated with ethanol and fluid shear stress].
OBJECTIVE
To detect whether ethanol can affect the expression of HSP70 in endothelial cells under fluid shear stress.


METHODS
Ethanol at different concentrations was added to the culture medium of endothelial cells, EA. Hy926, which was treated statically or under 1Pa fluid shear stress. After the incubation of 1 h, 2 h, 4 h and 6 h, the expression of HSP70 was detected by immunohistochemical method(SP).


RESULTS
In the control group, the expression of HSP70 was negative under static state, while it was positive under 1Pa fluid shear stress lasting 4 h even without ethanol. No statistic difference was found between the 50 mg/dL ethanol group and the control group with the same treatment time of fluid shear stress. HSP70 expression was found under static state with 150 mg/dL ethanol after 4 h or 300 mg/dL ethanol after 2 h respectively. The expression increased greatly under 1Pa fluid shear stress in the same ethanol concentrations.


CONCLUSIONS
Medium to high ethanol concentration in coordination with fluid shear stress can strongly stimulates the expression of HSP70 by a kinetic mechanism of time-dependent.
Yan
Hong-tao
HT
Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China.
Zhang
Yi
Y
Liao
Ga
G
Zhang
Kui
K
Li
Bin
B
Wang
Ye
Y
Liao
Zhi-gang
ZG
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D033081:Shear Strength; D013314:Stress, Mechanical
D018840:HSP70 Heat-Shock Proteins; D000431:Ethanol