title: Characterization of the type III export signal of the flagellar hook scaffolding protein FlgD of Escherichia coli.
abstract: Transport of flagellar structural proteins beyond the cytoplasmic membrane is accomplished by a type III secretory pathway [flagellar type III secretion system (fTTSS)]. The mechanism of substrate recognition by the fTTSS is still enigmatic. Using the hook scaffolding protein FlgD of Escherichia coli as a model substrate, it is demonstrated that the export signal is contained within the N-terminal 71 amino acids of FlgD. Analysis of frame-shift mutations and alterations of the nucleotide sequence suggest a proteinaceous nature of the signal. Furthermore, the physicochemical properties of the first about eight amino acids are crucial for export.
authors.affiliation: Fachbereich Biologie/Chemie, Abteilung Mikrobiologie, Universität Osnabrück, Barbarastrasse 11, 49069 Osnabrück, Germany.
authors.forename: Corinna
authors.initials: C
authors.lastname: Weber-Sparenberg
authors.forename: Petra
authors.initials: P
authors.lastname: Pöplau
authors.forename: Heiner
authors.initials: H
authors.lastname: Brookman
authors.forename: Maike
authors.initials: M
authors.lastname: Rochón
authors.forename: Carolin
authors.initials: C
authors.lastname: Möckel
authors.forename: Monika
authors.initials: M
authors.lastname: Nietschke
authors.forename: Heinrich
authors.initials: H
authors.lastname: Jung
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005407:Flagella / Q000378:metabolism*; D019897:Periplasm / Q000378:metabolism; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; C517779:FlgD protein, E coli; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D000469:Alkaline Phosphatase; C114089:phoA protein, E coli