Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor.
This chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine multidifferentiation potential of hESCs by differentiating into a specific type or random heterogenous cell populations in vitro. These approaches will become a fundamental platform to identify molecular networks regulating cell fate determination in hESCs and mouse embryonic stem cells.
Sato
Noboru
N
Laboratory of Molecular Embryology, The Rockefeller University, New York, NY, USA.
Brivanlou
Ali H
AH
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D018920:Coculture Techniques / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D017930:Genes, Reporter; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors*; D006801:Humans; D007150:Immunohistochemistry; D007211:Indoles / Q000494:pharmacology*; D008156:Luciferases / Q000235:genetics; D051379:Mice; D009474:Neurons / Q000166:cytology; D010091:Oximes / Q000494:pharmacology*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D015398:Signal Transduction / Q000187:drug effects; D017154:Stromal Cells / Q000166:cytology
C483321:6-bromoindirubin-3'-oxime; D004791:Enzyme Inhibitors; D007211:Indoles; D010091:Oximes; D008156:Luciferases; D038362:Glycogen Synthase Kinase 3