Growth inhibitory activity of wood of Taxus yunnanensis and its liquid chromatography Fourier-transform mass spectrometry analysis.
The wood of Taxus yunnanensis (Taxaceae) showed growth inhibitory activities against human cancer cell lines, such as cervical HeLa adenocarcinoma. The morphological changes indicated that the cellular growth inhibitions were caused by apoptosis. To determine the active components, T. yunnanensis wood was analyzed by using a liquid chromatography-Fourier-transform MS (LC/FT-MS) technique. As a result, taxane-type diterpenes, such as 10-deacetylcephalomannine and 10-deacetyltaxol, were found to be present in amounts consistent with the growth inhibitory activity.
Ueda
Jun-ya
JY
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Awale
Suresh
S
Tezuka
Yasuhiro
Y
Shimamura
Eriko
E
Hirai
Kei-ichi
K
Nobukawa
Takahiro
T
Sato
Akihiro
A
Kadota
Shigetoshi
S
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002853:Chromatography, Liquid; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005583:Fourier Analysis; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry; D024301:Plant Bark / Q000737:chemistry; D020946:Taxus / Q000737:chemistry*; D014934:Wood / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes