Inhibitory effects of Orostachys japonicus extracts on the formation of N-nitrosodimethylamine.
In Korea, Orostachys japonicus has been used traditionally as a drug and health food. The aim of this study was to investigate possible inhibitory effects of O. japonicus extracts on the formation of N-nitrosodimethylamines (NDMA). Chloroform extraction was the most effective method for recovering the highest number of phenolic compounds and flavonoids; in these extracts the greatest nitrite-scavenging activity and inhibition of NDMA formation occurred at pH 2.5. The chloroform extract was separated into 10 fractions (J1-J10); fraction J4 inhibited NDMA formation by 90.1 +/- 0.4%. This fraction was then separated into five subfractions (J4-1-J4-5) using a silica gel column. Subfractions J4-2 [(+)-catechin] and J4-4 (3,4-dihdroxybenzoic acid) inhibited NDMA formation by 89.5 +/- 0.9 and 77.6 +/- 0.8%, respectively.
Choi
Sun Young
SY
Department of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, South Korea.
Chung
Mi Ja
MJ
Seo
Woo Duck
WD
Shin
Jung Hye
JH
Shon
Mi Yae
MY
Sung
Nak Ju
NJ
D002273:Carcinogens / Q000037:antagonists & inhibitors* / Q000138:chemical synthesis; D002725:Chloroform; D029627:Crassulaceae / Q000737:chemistry*; D004128:Dimethylnitrosamine; D005419:Flavonoids / Q000494:pharmacology; D009573:Nitrites / Q000737:chemistry; D009602:Nitrosamines / Q000037:antagonists & inhibitors* / Q000138:chemical synthesis; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*
D002273:Carcinogens; D005419:Flavonoids; D009573:Nitrites; D009602:Nitrosamines; D010636:Phenols; D010936:Plant Extracts; D002725:Chloroform; D004128:Dimethylnitrosamine