title: Genetic segregation of microsatellite markers in Saccharum officinarum and S. spontaneum.
abstract: Genetic mapping techniques can be used to study the interaction between two different genomes after hybridization. This study investigated a Saccharum officinarum (Green German or GG, 2n approximately 11x approximately 110) x S. spontaneum (IND 81-146 or IND, 2n approximately 7x approximately 56) interspecific cross. Segregation of 193 microsatellite (SSR) loci was evaluated in the F(1) progeny of 169 full-sibs of the cross. Following the two-way pseudo-testcross strategy and 'cross pollination' population type, linkage groups (LG) and phases were established for each parent map, using the criteria of LOD score > or = 3.0 and a maximum recombination frequency of 0.35. Of the 193 markers analyzed, 61 were IND-specific, 106 were GG-specific, and 26 were heterozygous in both parents. About 78% of the markers segregated in a Mendelian fashion and 22% were distorted (as evaluated by chi(2)-tests, P < or = 0.01). The GG map included 91 marker loci arranged into 25 LG covering 1180 cM of the officinarum genome. The IND map consisted of 46 marker loci assembled into 10 LG, which spanned 614 cM of the spontaneum genome. A specific chromosome associated with segregation distortion was detected in the female (GG) genome only, probably as a result of double reduction. The segregation patterns of the marker loci indicated a centromere-driven distortion process with the shared allelic markers (as putative centromeres) regulating the placement and association of markers with opposite phase (coupling vs repulsion) and dosage on either side. Although incomplete, the framework maps were informative with respect to segregation distortion, chromosome fusion, rearrangements, and translocations, observed in both parental genomes as a result of their merger.
authors.affiliation: USDA-ARS Sugarcane Field Station, 12990 US Hwy 441 N., Canal Point, FL 33438, USA. sedme@saa.ars.usda.gov
authors.forename: S J
authors.initials: SJ
authors.lastname: Edmé
authors.forename: N G
authors.initials: NG
authors.lastname: Glynn
authors.forename: J C
authors.initials: JC
authors.lastname: Comstock
mesh_terms: D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D018745:Genome, Plant; D006824:Hybridization, Genetic; D018895:Microsatellite Repeats; D031786:Saccharum / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
chemical_list: D018744:DNA, Plant