Morin promotes the production of Th2 cytokine by modulating bone marrow-derived dendritic cells.
Our previous studies had reported that morin decreased the interleukin-12 (IL-12) and tumor necrosis factor-alpha (TNF-alpha) production in lipopolysaccharide (LPS)-activated macrophages, suggesting that morin may promote helper T type 2 (Th2) response in vivo. Dendritic cells (DCs) are the most potent antigen presenting cells and known to play a major role in the differentiation of helper T type 1 (Th1) and Th2 responses. This study aimed to reveal whether morin is able to control the Th differentiation through modulating the maturation and functions of DCs. Bone marrow-derived dendritic cells (BM-DCs) were incubated with various concentrations of morin and their characteristics were studied. The results indicated that morin significantly affects the phenotype and cytokine expression of BM-DCs. Morin reduced the production of IL-12 and TNF-alpha in BM-DCs, in response to LPS stimulation. In addition, the proliferative response of stimulated alloreactive T cells was significantly decreased by morin in BM-DCs. Furthermore, allogeneic T cells secreted higher IL-4 and lower IFN-gamma in response to morin in BM-DCs. In conclusion, these results suggested that morin favors Th2 cell differentiation through modulating the maturation and function of BM-DCs.
Li
Chia-Yang
CY
Institute of Medicine, School of Medicine, China Medical University, Taichung 404, Taiwan, ROC.
Suen
Jau-Ling
JL
Chiang
Bor-Luen
BL
Lee Chao
Pei-Dawn
PD
Fang
Shih-Hua
SH
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D018122:B7-1 Antigen / Q000032:analysis; D051940:B7-2 Antigen / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D039521:CD11c Antigen / Q000032:analysis; D019013:CD40 Antigens / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018418:Th2 Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D047309:Flavones
D000975:Antioxidants; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D039521:CD11c Antigen; D019013:CD40 Antigens; D016207:Cytokines; D005419:Flavonoids; D008070:Lipopolysaccharides; C008548:morin; D047309:Flavones