PACAP stimulates the release of the secretogranin II-derived peptide EM66 from chromaffin cells.
The aim of the present article was to examine the effect of PACAP on the release of the SgII-derived peptide EM66 from primary cultures of bovine chromaffin cells. PACAP dose dependently stimulated EM66 release from cultured chromaffin cells. A significant response was observed after 6 h of treatment with PACAP and increased to reach a 3.6-fold stimulation at 72 h. The stimulatory effect of PACAP was mediated through multiple signaling pathways, including calcium influx through L-type channels, PKA, PKC, and MAP-kinase cascades, to regulate EM66 release from chromaffin cells. These data suggest that EM66 may act downstream of the trans-synaptic stimulation of the adrenal medulla by neurocrine factors.
Guillemot
Johann
J
INSERM U413, Laboratory of Cellular and Molecular Neuroendocrinology, European Institute for Peptide Research (IFRMP23), University of Rouen, 76821 Mont-Saint-Aignan, France.
Ait-Ali
Djida
D
Turquier
Valerie
V
Montero-Hadjadje
Maite
M
Fournier
Alain
A
Vaudry
Hubert
H
Anouar
Youssef
Y
Yon
Laurent
L
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000187:drug effects* / Q000378:metabolism*; D002864:Chromogranins / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000494:pharmacology*; D053381:Secretogranin II / Q000494:pharmacology*
D002864:Chromogranins; C557436:EM66 peptide; D010446:Peptide Fragments; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D053381:Secretogranin II