[Experimental study on effect of er'zhi tiangui granule in improving quality of oocyte and its correlation with level of insulin-like growth factor-1R mRNA expression in ovary of mice].
OBJECTIVE
To explore the mechanism of Er'zhi Tiangui Granule (ETG) in improving the quality of oocyte.


METHODS
Ninety mice were randomly divided into 6 groups. The number of high-quality oocytes was comparatively observed in the 1st experimental group and the 1st control group; the embryonic cleavage rate was observed in the 2nd experimental group and the 2nd control group and the quantity of insulin-like growth factor-1R mRNA (IGF-1R mRNA) expression in ovarian granular cells was determined by using in situ hybridization in the 3rd experimental group and the 3rd control group.


RESULTS
The high-quality oocytes rate, the embryonic cleavage rate and the quantity of IGF-1R mRNA expression in the three paired groups was (78 +/- 8)% vs (71 +/- 5)%, (88 +/- 3)% vs (83 +/- 5)%, 0.4890 +/- 0.0454 vs 0.4439 +/- 0.0283, respectively. The difference between the experimental groups to the respective control groups was significant (all P < 0.05), and positive correlation was shown between the high-quality oocytes rate and the quantity of IGF-1R mRNA expression.


CONCLUSIONS
The mechanism of ETG in improving the quality of oocyte may be related with the elevation of IGF-1R mRNA level in ovarian granular cells.
Lian
Fang
F
Reproductive Center, Affiliated Hospital of Shandong University of TCM, Jinan. flian@chinaivf.com
Sun
Zhen-gao
ZG
Mu
Lin
L
D000818:Animals; D002970:Cleavage Stage, Ovum / Q000502:physiology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression; D008297:Male; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010053:Ovary / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D017526:Receptor, IGF Type 1