Aeromonas sharmana sp. nov., isolated from a warm spring.
A Gram-negative, facultatively anaerobic bacterial strain designated GPTSA-6(T) was isolated from a water sample collected from a warm spring in Assam, India. Preliminary analysis of the 16S rRNA gene sequence of this isolate revealed its affiliation to the family Aeromonadaceae. Detailed characterization using a polyphasic approach indicated that strain GPTSA-6(T) is most closely related to Aeromonas sobria but differs significantly from existing members of the genus Aeromonas. Analysis of the almost-complete (1430 nt) 16S rRNA gene sequence of this strain revealed that its closest relative (99.23 % similarity) is an uncultured bacterial clone, A-8, isolated from an algal bloom. Of the taxa with validly published names, Aeromonas sobria ATCC 43979(T) showed the highest level of sequence similarity (95.13 %) with respect to strain GPTSA-6(T), followed by Aeromonas molluscorum 848T(T) and Aeromonas popoffii LMG 17541(T) (95.04 % similarity in both cases). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, it can be concluded that strain GPTSA-6(T) represents a novel species of the genus Aeromonas, for which the name Aeromonas sharmana sp. nov. is proposed. The type strain is GPTSA-6(T) (=MTCC 7090(T)=DSM 17445(T)).
Saha
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P
Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
Chakrabarti
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T
Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
D000333:Aeromonas / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014871:Water Microbiology
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S