[Cloning of distinguishing DNA sequences of Gastrodia elata Blume and application of them in identifying gastrodia tuber].
Gastrodia elata Bl. is a famous and costful traditional Chinese medicine. Their genomic DNA fingerprints were investigated using a modified Randomly Amplified Polymorphic DNA method. DNA fragments common to all or to fine populations were identified and recovered. Five DNA fragments were proven not to be reported through DNA cloning, PCR identifying, nucleotide sequencing and bioinformatics analyses and were received in and recorded by NCBI GenBank. Gastrodine contents of the Gastrodia tuber samples were determined using high performance liquid chromatography technique. The distribution of the five DNA fragments in 9 Gastrodia elata Blue populations and the correlation with gastromedicine content were studied. The results show the distribution of these DNA sequences varied greatly among the populations whereby DNA Sequence 1 was the common and distinguishing molecular marker for all the populations studied and DNA Sequence 2 may relate to higher gastrodine content. In conclusion, these DNA marker sequences can be employed to identify genuine gastrodia tubers, better varieties and optimize their selection and cultivating.
Tao
Jun
J
Bioengineering and Food Engineering School, Changsha University of Science and Technology, Changsha 410076, China.
Fu
Tie-Xiang
TX
Luo
Zhi-Yong
ZY
Wen
Li
L
Wang
Zhi-Cheng
ZC
Shu
Xiao-Shun
XS
Liu
Shui-Ping
SP
Tao
Yao
Y
Hu
Wei-Xin
WX
D001483:Base Sequence; D001592:Benzyl Alcohols / Q000032:analysis; D003001:Cloning, Molecular; D019295:Computational Biology; D018744:DNA, Plant / Q000737:chemistry*; D031668:Gastrodia / Q000235:genetics*; D005960:Glucosides / Q000032:analysis; D035281:Plant Tubers / Q000235:genetics
D001592:Benzyl Alcohols; D018744:DNA, Plant; D005960:Glucosides; C045345:gastrodin