Methylmercury determination in biological samples using electrothermal atomic absorption spectrometry after acid leaching extraction.
An efficient and sensitive method for the determination of methylmercury in biological samples was developed based on acid leaching extraction of methylmercury into toluene. Methylmercury in the organic phase was determined by electrothermal atomic absorption spectrometry (ETAAS). The methylmercury signal was enhanced and the reproducibility increased by formation of certain complexes and addition of Pd-DDC modifier. The complex of methylmercury with DDC produced the optimum analytical signal in terms of sensitivity and reproducibility compared to complexes with dithizone, cysteine, 1,10-phenanthroline, and diethyldithiocarbamate. Method performance was optimized by modifying parameters such as temperature of mineralization, atomization, and gas flow rate. The limit of detection for methylmercury determination was 0.015 mug g(-1) and the RSD of the whole procedure was 12% for human teeth samples (n=5) and 15.8% for hair samples (n=5). The method's accuracy was investigated by using NIES-13 and by spiking the samples with different amounts of methylmercury. The results were in good agreement with the certified values and the recoveries were 88-95%.
Saber-Tehrani
Mohammad
M
Department of Chemistry, Science and Research Branch, Islamic Azad University, P.O. Box 1415-775, Tehran, Iran. drmsabertehrani@yahoo.com
Hashemi-Moghaddam
Hamid
H
Givianrad
Mohammad Hadi
MH
Abroomand-Azar
Parviz
P
D000143:Acids / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006197:Hair / Q000737:chemistry; D006801:Humans; D008767:Methylmercury Compounds / Q000032:analysis*; D009942:Organometallic Compounds / Q000737:chemistry; D010165:Palladium / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013054:Spectrophotometry, Atomic / Q000379:methods / Q000592:standards; D013696:Temperature; D014050:Toluene / Q000737:chemistry; D014070:Tooth / Q000737:chemistry
D000143:Acids; D008767:Methylmercury Compounds; D009942:Organometallic Compounds; D014050:Toluene; D010165:Palladium; D006108:Graphite