Transpupillary thermotherapy-induced modification of angiogenesis- and coagulation-related gene expression in the rat posterior fundus.
OBJECTIVE
To study gene expression changes in the rat retina and choroid following transpupillary thermotherapy (TTT) and to identify molecular mechanisms that may enhance treatment of choroidal neovascularization, complicating age-related macular degeneration.


METHODS
One fundus of Brown Norway rats was treated with an 810 nm diode laser while the contralateral fundus received no treatment. The mRNA was extracted and processed for cDNA microarray analysis. Genes with increased expression were validated by semiquantitative reverse transcription polymerase chain reaction (PCR) and quantitative real-time PCR (qRT-PCR).


RESULTS
Of the 14,815 cDNA elements on the array, 12 genes were up-regulated in TTT treated eyes. Upregulation of eight of these 12 genes could be verified by semiquantitative RT-PCR. The eight verified genes were EPCR, IL-1beta, MCP-1, TSP-1, Fgl, Asns, MT-2, and NMDMC, which included 4 angiogenesis- and coagulation-related genes.


CONCLUSIONS
This study demonstrates upregulation of angiogenesis- and coagulation-related genes following TTT. The response profile and its temporal relationships provide insight into the molecular mechanisms that lead to vascular occlusion and antiangiogenesis induced by TTT.
Ito
Yoko N
YN
Department of Ophthalmology, Saitama Medical University, Iruma, Saitama, Japan.
Ito
Mitsuteru
M
Takita
Hiroyasu
H
Yoneya
Shin
S
Peyman
Gholam A
GA
Gehlbach
Peter L
PL
Mori
Keisuke
K
D000818:Animals; D001777:Blood Coagulation / Q000235:genetics*; D002829:Choroid / Q000378:metabolism; D020256:Choroidal Neovascularization / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006979:Hyperthermia, Induced; D020411:Oligonucleotide Array Sequence Analysis; D011680:Pupil; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011914:Rats, Inbred BN; D012160:Retina / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D012333:RNA, Messenger