Resident neuroelectrochemical interfacing using carbon nanofiber arrays.
Carbon nanofiber electrode architectures are used to provide for long-term, neuroelectroanalytical measurements of the dynamic processes of intercellular communication between excitable cells. Individually addressed, vertically aligned carbon nanofibers are incorporated into multielement electrode arrays upon which excitable cell matrixes of both neuronal-like derived cell lines (rat pheochromocytoma, PC-12) and primary cells (dissociated cells from embryonic rat hippocampus) are cultured over extended periods (days to weeks). Electrode arrays are characterized with respect to their response to easily oxidized neurotransmitters, including dopamine, norepinephrine, and 5-hydroxytyramide. Electroanalysis at discrete electrodes following long-term cell culture demonstrates that this platform remains responsive for the detection of easily oxidized species generated by the cultured cells. Preliminary data also suggests that quantal release of easily oxidized transmitters can be observed at nanofiber electrodes following direct culture and differentiation on the arrays for periods of at least 16 days.
McKnight
Timothy E
TE
Monolithic Systems Development Group, Oak Ridge National Laboratory, Molecular Scale Engineering and Nanoscale Technologies Research Group, Oak Ridge, Tennessee 37831-6006, USA. mcknightte@ornl.gov
Melechko
Anatoli V
AV
Fletcher
Benjamin L
BL
Jones
Stephen W
SW
Hensley
Dale K
DK
Peckys
Diana B
DB
Griffin
Guy D
GD
Simpson
Michael L
ML
Ericson
M Nance
MN
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D004563:Electrochemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008839:Microelectrodes; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D012680:Sensitivity and Specificity; D013997:Time Factors
D037742:Nanotubes, Carbon