Gene expression changes after hypoxic preconditioning in rat hepatocytes.
BACKGROUND
Hypoxic preconditioning can protect hepatocytes against hypoxic injury, but its mechanism has not been elucidated. The aim of this study was to profile gene expression patterns involved in hypoxic preconditioning and probable mechanism at the level of gene expression.


METHODS
Hepatocytes were divided into 2 groups: control group and hypoxic preconditioning group. Biotin-labeled cRNA from the control group and the hypoxic preconditioning group was hybridized by oligonucleotide microarray. Genes that were significantly associated with hypoxic preconditioning were filtered, and validated at the level of transcript expression.


RESULTS
Forty-three genes with significantly altered expression patterns were discovered and most of them had not been previously reported. Among these genes, genes encoding superoxide dismutase 2 (SOD2) and interleukin 10 (IL-10) in the hypoxic preconditioning group were confirmed to be up-regulated with real-time quantitative PCR.


CONCLUSIONS
Many cytokines are involved in hypoxic preconditioning and protect hepatocytes from hypoxia-reoxygenation injury, and the increase of oxygen free-radical scavengers and anti-inflammatory factors may play a key role in this phenomenon. Diverse signal pathways are probably involved.
Chen
Wei
W
Department of General Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
Qiu
Jiang-Feng
JF
Zhang
Zhi-Qi
ZQ
Luo
Hai-Feng
HF
Rosello-Catafau
Joan
J
Wu
Zhi-Yong
ZY
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D015870:Gene Expression; D016753:Interleukin-10 / Q000235:genetics; D019194:Ischemic Preconditioning; D008099:Liver / Q000098:blood supply*; D008297:Male; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000235:genetics
D017931:DNA Primers; D016753:Interleukin-10; D013482:Superoxide Dismutase