Insulin resistance does not influence gene expression in skeletal muscle.
Insulin resistance is commonly observed in patients prior to the development of type 2 diabetes and may predict the onset of the disease. We tested the hypothesis that impairment in insulin stimulated glucose-disposal in insulin resistant patients would be reflected in the gene expression profile of skeletal muscle. We performed gene expression profiling on skeletal muscle of insulin resistant and insulin sensitive subjects using microarrays. Microarray analysis of 19,000 genes in skeletal muscle did not display a significant difference between insulin resistant and insulin sensitive muscle. This was confirmed with real-time PCR. Our results suggest that insulin resistance is not reflected by changes in the gene expression profile in skeletal muscle.
Nguyen
Lisa L
LL
School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW, Australia. L.Nguyen@mmb.usyd.edu.au
Kriketos
Adamandia D
AD
Hancock
Dale P
DP
Caterson
Ian D
ID
Denyer
Gareth S
GS
D000328:Adult; D001706:Biopsy; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D052097:Quadriceps Muscle / Q000473:pathology / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007328:Insulin