title: Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins.
abstract: Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads.
authors.affiliation: National Creative Research Initiative Center for Oxide Nanocrystalline Materials and School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea.
authors.forename: In Su
authors.initials: IS
authors.lastname: Lee
authors.forename: Nohyun
authors.initials: N
authors.lastname: Lee
authors.forename: Jongnam
authors.initials: J
authors.lastname: Park
authors.forename: Byung Hyo
authors.initials: BH
authors.lastname: Kim
authors.forename: Yong-Weon
authors.initials: YW
authors.lastname: Yi
authors.forename: Taeuk
authors.initials: T
authors.lastname: Kim
authors.forename: Tae Kook
authors.initials: TK
authors.lastname: Kim
authors.forename: In Hwan
authors.initials: IH
authors.lastname: Lee
authors.forename: Seung R
authors.initials: SR
authors.lastname: Paik
authors.forename: Taeghwan
authors.initials: T
authors.lastname: Hyeon
mesh_terms: D006639:Histidine / Q000737:chemistry*; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D006639:Histidine; D009532:Nickel; C028007:nickel monoxide