The antitumor and antioxidative activities of polysaccharides isolated from Isaria farinosa B05.
The water-soluble intra-polysaccharides WIPS1 and water-soluble extra-polysaccharides WEPS1 were isolated from Isaria farinosa B05 through ethanol precipitation and gel permeation chromatography (GPC). Their characteristics were determined by chemical analysis, gas chromatography, GPC and IR spectroscopy. The results show that WIPS1 contained 90.3% carbohydrate, 8.00% uronic acid, 7.15% protein and three kinds of monosaccharides including mannose, galactose and glucose with a molar ratio of 8.0:4.8:1.0. WEPS1 contained 93.4% carbohydrate, 8.06% uronic acid, 4.40% protein and three kinds of monosaccharides including mannose, galactose and glucose with a molar ratio of 21.6:4.7:1.0. WIPS1 and WEPS1 had a molecular weight of 42 and 208kDa, respectively. The in vivo tests in mice indicate that WIPS1 and WEPS1 had significant antitumor and antioxidative activities to some extent.
Jiang
Yan-Hua
YH
Institute of Food Science and Engineering, Division of Life Science and Technology, Ocean University of China, Qingdao, PR China. yanhuajiang777@hotmail.com <yanhuajiang777@hotmail.com>
Jiang
Xiao-Lu
XL
Wang
Peng
P
Mou
Hai-Jin
HJ
Hu
Xiao-Ke
XK
Liu
Shu-Qing
SQ
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002241:Carbohydrates / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D005591:Chemical Fractionation; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D005260:Female; D006999:Hypocreales / Q000737:chemistry*; D008099:Liver / Q000201:enzymology; D051379:Mice; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis; D012509:Sarcoma / Q000517:prevention & control; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000378:metabolism; D014574:Uronic Acids / Q000032:analysis
D000970:Antineoplastic Agents; D000975:Antioxidants; D002241:Carbohydrates; D011134:Polysaccharides; D011506:Proteins; D014574:Uronic Acids; D002374:Catalase; D013482:Superoxide Dismutase