title: Alistipes onderdonkii sp. nov. and Alistipes shahii sp. nov., of human origin.
abstract: Two groups of previously unknown Gram-negative, strictly anaerobic, pigment-producing, rod-shaped bacteria, which phenotypically and phylogenetically displayed a close association with the recently described species Alistipes finegoldii, were characterized using phenotypic and molecular taxonomic methods. A 16S rRNA gene sequence divergence of approximately 3 % between the two unknown bacteria and A. finegoldii, as well as distinguishable biochemical characteristics, demonstrates that these organisms are genotypically and phenotypically distinct and that each group represents a previously unknown subline within the genus Alistipes. Chromosomal DNA-DNA reassociation studies further confirmed the separateness of the unidentified bacteria and A. finegoldii. On the basis of the phenotypic and phylogenetic findings, two novel species, Alistipes onderdonkii sp. nov. and Alistipes shahii sp. nov., are proposed. The type strains of A. onderdonkii and A. shahii are WAL 8169(T) (=CCUG 48946(T)=ATCC BAA-1178(T)) and WAL 8301(T) (=CCUG 48947(T)=ATCC BAA-1179(T)), respectively; their DNA G+C contents are 58 and 56 mol%, respectively.
authors.affiliation: Research Service, VA Medical Center West Los Angeles, Building 304, Room E3-227, 11301 Wilshire Boulevard, Los Angeles, CA 90073, USA.
authors.forename: Yuli
authors.initials: Y
authors.lastname: Song
authors.affiliation: Anaerobe Reference Laboratory, National Public Health Institute (KTL), Helsinki, Finland.
authors.forename: Eija
authors.initials: E
authors.lastname: Könönen
authors.affiliation: Anaerobe Reference Laboratory, National Public Health Institute (KTL), Helsinki, Finland.
authors.forename: Merja
authors.initials: M
authors.lastname: Rautio
authors.affiliation: Research Service, VA Medical Center West Los Angeles, Building 304, Room E3-227, 11301 Wilshire Boulevard, Los Angeles, CA 90073, USA.
authors.forename: Chengxu
authors.initials: C
authors.lastname: Liu
authors.affiliation: Anaerobe Reference Laboratory, National Public Health Institute (KTL), Helsinki, Finland.
authors.forename: Anne
authors.initials: A
authors.lastname: Bryk
authors.affiliation: Department of Medical Microbiology, University of Turku, Turku, Finland.
authors.forename: Erkki
authors.initials: E
authors.lastname: Eerola
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, UCLA School of Medicine, Los Angeles, CA, USA.|Department of Medicine, UCLA School of Medicine, Los Angeles, CA, USA.|Infectious Diseases Section, VA Medical Center West Los Angeles, Los Angeles, CA 90073, USA.
authors.forename: Sydney M
authors.initials: SM
authors.lastname: Finegold
mesh_terms: D041963:Bacteroidetes / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S