Gcn5p contributes to the bidirectional character of the UGA3-GLT1 yeast promoter.
Analysis of the UGA3-GLT1 bidirectional promoter has indicated that its transcriptional activation is determined by the combined action of Gcn4p and Gln3p, and that its bidirectional character is influenced by chromatin organization, through the action of an Abf1p binding site and a polydAdTtract. Results presented in this paper show that lack of Gcn5p impairs histone acetylation and nucleosomal organization of the UGA3-GLT1 promoter, resulting in an asymmetrical transcriptional activation response of UGA3 and GLT1. The phenotype displayed by a double mutant impaired in GCN5 and in the Abf1p binding site indicates that the combined action of these two elements determines the bidirectional capacity of the UGA3-GLT1 intergenic region.
Aranda
Cristina
C
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, 04510México D.F., Mexico.
Colón
Maritrini
M
Ishida
Cecilia
C
Riego
Lina
L
Deluna
Alexander
A
Valenzuela
Lourdes
L
Herrera
Jorge
J
González
Alicia
A
D000107:Acetylation; D001665:Binding Sites / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D027342:Excitatory Amino Acid Transporter 2 / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D051548:Histone Acetyltransferases / Q000737:chemistry / Q000502:physiology*; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000099224:p300-CBP-Associated Factor
D021901:DNA, Intergenic; D004268:DNA-Binding Proteins; D027342:Excitatory Amino Acid Transporter 2; D051548:Histone Acetyltransferases; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C061886:ABF1 protein, S cerevisiae; C092661:UGA3 protein, S cerevisiae; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor