Zebrafish promoter microarrays identify actively transcribed embryonic genes.
We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.
Wardle
Fiona C
FC
Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, Cambridge University, Cambridge, UK. fcw27@cam.ac.uk
Odom
Duncan T
DT
Bell
George W
GW
Yuan
Bingbing
B
Danford
Timothy W
TW
Wiellette
Elizabeth L
EL
Herbolsheimer
Elizabeth
E
Sive
Hazel L
HL
Young
Richard A
RA
Smith
James C
JC
D000818:Animals; D047369:Chromatin Immunoprecipitation; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005796:Genes / Q000235:genetics*; D023281:Genomics / Q000379:methods; D006657:Histones / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism
D006657:Histones