title: Contribution of alpha2beta1, alpha3beta1, alpha6beta4 integrins and 67 kDa laminin receptor to the interaction of epidermoid carcinoma A-431 cells with laminin-2/4.
abstract: Laminin-2/4 is the major laminin isoform of normal muscle and nerve tissues and plays an important role in tumor invasion and metastasis. Despite the fact that laminin-2/4 has been found in the skin basement membrane, insufficient evidence is available on the effect of laminin-2/4 on the behavior of both normal and transformed skin cells. A comparison of the contribution of alpha2beta1, alpha3beta1, alpha6beta4 integrins and 67 kDa laminin receptor on the surface of the human epidermoid carcinoma cell, A-431, to interaction with laminin-2/4 was carried out. The cell interaction with extracellular matrix component is a multistage process. We employed new methods for studying different stages of the interaction of A-431 cells with laminin-2/4. We demonstrated that integrins alpha2beta1, alpha3beta1, alpha6beta4 and 67 kDa laminin receptor are involved in the interaction of A-431 cells with laminin-2/4. We found that contribution of the same receptors to different stages of the interaction with laminin can be different. alpha2beta1 integrins are involved in EGF-induced A-431 cells' migration on laminin-2/4. We demonstrated the cooperation between alpha2beta1 and alpha3beta1 integrins during adhesion and spreading of A-431 cells on laminin-2/4-coated substrate. These results provide information about laminin-2/4 receptors and their contribution to different stages of the interaction with cells.
authors.affiliation: Department of Cell Culture, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia. oac4x@virginia.edu
authors.forename: Olga A
authors.initials: OA
authors.lastname: Cherepanova
authors.forename: Natalia
authors.initials: N
authors.lastname: Kalmykova
authors.forename: Yury P
authors.initials: YP
authors.lastname: Petrov
authors.forename: Miralda
authors.initials: M
authors.lastname: Blinova
authors.forename: George
authors.initials: G
authors.lastname: Pinaev
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D016764:Cell Polarity / Q000187:drug effects; D006801:Humans; D038982:Integrin alpha2beta1 / Q000276:immunology / Q000502:physiology*; D039201:Integrin alpha3beta1 / Q000276:immunology / Q000502:physiology*; D039161:Integrin alpha6beta4 / Q000276:immunology / Q000502:physiology*; D007797:Laminin / Q000378:metabolism*; D051379:Mice; D017474:Receptors, Laminin / Q000502:physiology*; D012878:Skin Neoplasms / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D038982:Integrin alpha2beta1; D039201:Integrin alpha3beta1; D039161:Integrin alpha6beta4; D007797:Laminin; D017474:Receptors, Laminin; C102645:laminin alpha 2