title: [Application of bronchoscopic lung volume reduction using one-way flap device in sheep model of heterogeneous emphysema].
abstract: OBJECTIVE
  To evaluate the effectiveness of bronchoscopic lung volume reduction (BLVR) using one way flap device in sheep model of heterogeneous emphysema.
  
  
  METHODS
  Six 6-month sheep (weight: 20-30 kg) were treated with localized papain instillations to generate heterogeneous emphysema, subsequently underwent BLVR using one way-flap device at subsegment. Lung functional residual capacity (FRC) was analyzed before and 8 weeks after operation. Animals were euthanized at the 8-week time point. Lungs were removed en bloc and inflated with a super syringe to look for areas of gross collapse. Samples were collected from collapsed and non-collapsed areas, fixed in 10% buffered formalin, made paraffin section and stained with hematoxylin-eosin (HE) to observe morphologic change of bronchi.
  
  
  RESULTS
  BLVR was well tolerated without complications, and it reduced lung volumes (change in residual volume 49.5%). There was no evidence of infection, abscess, or granuloma formation, or allergic reaction. Scar tissue, generated by BLVR, replaced hyperinflated lung,improved respiratory function safely and consistently.
  
  
  CONCLUSIONS
  BLVR using one-way flap device is a minimally invasive procedure and the stimulus of the devices to walls of bronchi is slight, moreover, the technique may attain effectiveness of surgery. Therefore maybe it will be a perspective treatment of chronic obstructive pulmonary disease.
authors.affiliation: Tianjin Institute of Respiratory Disease, Tianjin Haihe Hospital, Tianjin 300350, China. wqw572004@yahoo.com.cn
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: Jun-ping
authors.initials: JP
authors.lastname: Wu
authors.forename: Yong
authors.initials: Y
authors.lastname: Fan
authors.forename: Chun-bao
authors.initials: CB
authors.lastname: Liang
authors.forename: Jing-liang
authors.initials: JL
authors.lastname: Ma
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Jing-na
authors.initials: JN
authors.lastname: Hua
authors.forename: Yong-mei
authors.initials: YM
authors.lastname: Wang
authors.forename: Jin-rong
authors.initials: JR
authors.lastname: Wang
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Li
authors.forename: Bao-xiang
authors.initials: BX
authors.lastname: Ren
mesh_terms: D000818:Animals; D001999:Bronchoscopy; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000473:pathology; D011013:Pneumonectomy / Q000379:methods*; D011656:Pulmonary Emphysema / Q000473:pathology / Q000601:surgery*; D012756:Sheep; D015607:Stents