Requirement for coronin 1 in T lymphocyte trafficking and cellular homeostasis.
The evolutionarily conserved actin-related protein (Arp2/3) complex is a key component of actin filament networks that is dynamically regulated by nucleation-promoting and inhibitory factors. Although much is known about actin assembly, the physiologic functions of inhibitory proteins are unclear. We generated coronin 1-/- mice and found that coronin 1 exerted an inhibitory effect on cellular steady-state F-actin formation via an Arp2/3-dependent mechanism. Whereas coronin 1 was required for chemokine-mediated migration, it was dispensable for T cell antigen receptor functions in T cells. Moreover, actin dynamics, through a mitochondrial pathway, was linked to lymphocyte homeostasis.
Föger
Niko
N
Department of Immunology, Genentech, Incorporated, 1 DNA Way, South San Francisco, CA 94080, USA.
Rangell
Linda
L
Danilenko
Dimitry M
DM
Chan
Andrew C
AC
D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D019264:Adoptive Transfer; D000818:Animals; D017209:Apoptosis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000502:physiology; D016764:Cell Polarity; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte; D018390:Gene Targeting; D006706:Homeostasis; D007425:Intracellular Membranes / Q000502:physiology; D008198:Lymph Nodes / Q000276:immunology; D008564:Membrane Potentials; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000235:genetics / Q000502:physiology*; D008928:Mitochondria / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D016608:Talin / Q000378:metabolism
D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D008840:Microfilament Proteins; D011948:Receptors, Antigen, T-Cell; D016608:Talin; C072061:coronin proteins