FABP3 and FABP10 in Atlantic salmon (Salmo salar L.)--general effects of dietary fatty acid composition and life cycle variations.
The increased use of dietary plant oil supplementation combined with high dietary lipid loads challenges the lipid transport systems of cultivated fish species. Fatty acid binding proteins (FABPs) have been thoroughly studied as intracellular fatty acid transporters in vertebrates, but no data have been reported in Atlantic salmon. In the present study, comparative characterizations were performed, and dietary influence of plant oil supplementation on FABP3 and FABP10 expression was studied for several tissues in two separate dietary trials. In trial I, groups (6 fish each) were fed diets for 42 weeks (body mass 142+/-1 to 1463+/-83 g) (mean+/-S.D.), containing graded levels of rapeseed oil substituting for fish oil using a linear regression design. In trial II, groups (3 fish each) were fed 100% fish oil or 100% plant oil for 22 months (0.160+/-0.052 to 2523+/-590 g) (mean+/-S.D.) and sampled at regular intervals. Liver and muscle tissues appeared to express several FABPs possibly linked to different metabolic functions. FABPs mRNA expression did not change with dietary inclusion of 75% rapeseed oil, whereas FABP3 protein expression seemed to be affected by dietary rapeseed oil inclusion. Significant changes in red muscle FABP3 mRNA expression correlate to significant changes in total beta-oxidation capacity during the energy consuming process of smoltification.
Jordal
Ann-Elise O
AE
National Institute of Nutrition and Seafood Research, P.O. Box 2029 Nordnes, N-5817 Bergen, Norway. ann-elise.jordal@nifes.no
Hordvik
Ivar
I
Pelsers
Maurice
M
Bernlohr
David A
DA
Torstensen
Bente E
BE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004041:Dietary Fats / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D008018:Life Cycle Stages / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction; D010938:Plant Oils / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000074262:Rapeseed Oil; D020317:Salmo salar / Q000254:growth & development*; D013997:Time Factors
D004041:Dietary Fats; D050556:Fatty Acid-Binding Proteins; D005229:Fatty Acids, Monounsaturated; D029941:Fish Proteins; D010938:Plant Oils; D012333:RNA, Messenger; D000074262:Rapeseed Oil