HPLC determination of omeprazole in human plasma using a monolithic column.
A rapid and sensitive HPLC method using a monolithic column has been developed for quantification of omeprazole (CAS 73590-58-6) in plasma. The method was specific and sensitive with a quantification limit of 10 ng/ml. Sample preparation involves simple, one-step extraction procedure and analytical recovery was complete. The separation was carried out in reversed-phase conditions using a Chromolith Performance (RP-18e, 100 x 4.6 mm) column with an isocratic mobile phase consisting of 0.01 mol/l disodium hydrogen phosphate buffer-acetonitrile (73:27 v/v) adjusted to pH 7.1. The wavelength was set at 302 nm. The calibration curve was linear over the concentration range 20-1500 ng x ml(-1). The coefficients of variation for inter-day and intra-day assay were found to be less than 7%.
Zarghi
Afshin
A
Department of Pharmaceutical Chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, No. 10, Shams Alley, across from Tavanir St., Vali-e-Asr Ave., P.O. Box 14155-6153, Tehran Iran. azarghi@yahoo.com
Foroutan
Seyyed Mohsen
SM
Shafaati
Alireza
A
Khoddam
Arash
A
D000328:Adult; D000897:Anti-Ulcer Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D009853:Omeprazole / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000897:Anti-Ulcer Agents; D009853:Omeprazole