[Three-dimensional spheroid model for cultivating WB-F344 cells in simulated microgravity].
Three-dimensional (3D) culture of cells could closely mimic the in vivo situation with regard to cell function and microenvironment compared with plane monolayer cultured cells. In this paper, we established 3D culture of rat WB-F344 cells with rotary cell culture system (RCCS) to simulate microgravity environment, and examined cells proliferation, morphology, microstructure, E-cadherin protein quantity and mRNA expression of adhesion molecules by count the number of cells, optical microscope, transmission electron microscope and reverse transcriptase-polymerase chain reaction (RT-PCR). The results demonstrated that cells were polyhedron with lots of micovilli and mitochondria, which grow well and packed together densely to form irregular aggregates. Adjacent cells were connected with desmosome and tight junction. With the regard, the aggregates behaved 3D growth characteristics. Moreover, compared with control, mRNA level of Fibronectin and E-cadherin protein were increased, the changes maybe is the part mechanism in this microgravity simulated cells culture models which strengthened cells junction. This rotating 3D model might facilitate the study of interactions of cell-cell, cell-matrix and the mechanisms.
Qu
Xin-Jian
XJ
Animal Science Department of Northwest Sci., Tech University of Agriculture and Forestry, Yangling 712100, China.
Li
Hui-Xia
HX
Sun
Shi-Duo
SD
Feng
Mei-Fu
MF
D000818:Animals; D015820:Cadherins / Q000235:genetics; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D005353:Fibronectins / Q000235:genetics; D051381:Rats; D018874:Spheroids, Cellular / Q000648:ultrastructure; D018474:Weightlessness Simulation
D015820:Cadherins; D005353:Fibronectins