Biocompatible and biodegradable phosphorylcholine ionomers with reduced protein adsorption and cell adhesion.
In this paper a recently developed biodegradable phosphorylcholine ionomer (PC ionomer) was evaluated in different biological environments with a focus on the adsorption of proteins (fibrinogen) and the adhesion of cells. Our results have shown that the polar phosphoryl choline (PC) group may be enriched at the surface of cast films with an added hydrophilic environment. X-ray photoelectron spectroscopy confirmed the surface depletion of PC groups in dry conditions, as nitrogen and phosphorous atoms were found in the bulk of the material but not at the outermost surface layer. The surface enrichment leads to a strongly hydrophilic surface that prevents the adsorption of proteins and reduces the adhesion of cells. The non-functional and hydrophobic reference poly(trimethylene carbonate) (PTMC) adsorbs both proteins and cells, thus the wetting and low adhesion behavior of the PC ionomer can be attributed to the introduced PC functionality. Since the in vivo acceptance of biomaterials is determined by their ability to withstand protein adsorption the PC ionomer described in this paper is highly interesting for a number of in vivo applications in which the adsorption of proteins may be critical, for example, blood contact events.
Nederberg
Fredrik
F
Department of Materials Chemistry, Polymer Chemistry, Box 538, Uppsala University, S-751 21 Uppsala, Sweden.
Watanabe
Junji
J
Ishihara
Kazuhiko
K
Hilborn
Jöns
J
Bowden
Tim
T
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001673:Biodegradation, Environmental; D002254:Carbonates / Q000737:chemistry*; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line; D005340:Fibrinogen; D066298:In Vitro Techniques; D008422:Materials Testing; D051379:Mice; D015394:Molecular Structure; D010767:Phosphorylcholine / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*
D001672:Biocompatible Materials; D002254:Carbonates; D011091:Polyesters; C401957:poly(tetramethylene carbonate); D010767:Phosphorylcholine; D005340:Fibrinogen