title: Structural characterization of mumps virus fusion protein core.
abstract: The fusion proteins of enveloped viruses mediating the fusion between the viral and cellular membranes comprise two discontinuous heptad repeat (HR) domains located at the ectodomain of the enveloped glycoproteins. The crystal structure of the fusion protein core of Mumps virus (MuV) was determined at 2.2 A resolution. The complex is a six-helix bundle in which three HR1 peptides form a central highly hydrophobic coiled-coil and three HR2 peptides pack against the hydrophobic grooves on the surface of central coiled-coil in an oblique antiparallel manner. Fusion core of MuV, like those of simian virus 5 and human respiratory syncytium virus, forms typical 3-4-4-4-3 spacing. The similar characterization in HR1 regions, as well as the existence of O-X-O motif in extended regions of HR2 helix, suggests a basic rule for the formation of the fusion core of viral fusion proteins.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Yueyong
authors.initials: Y
authors.lastname: Liu
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lou
authors.forename: Jieqing
authors.initials: J
authors.lastname: Zhu
authors.forename: Xuebo
authors.initials: X
authors.lastname: Hu
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
authors.forename: Bingsheng
authors.initials: B
authors.lastname: Qiu
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
authors.forename: Po
authors.initials: P
authors.lastname: Tien
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D008561:Membrane Fusion / Q000502:physiology*; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000737:chemistry* / Q000472:pathogenicity; D045402:Parainfluenza Virus 5 / Q000737:chemistry / Q000472:pathogenicity; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D020449:Repetitive Sequences, Amino Acid; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000502:physiology
chemical_list: D010446:Peptide Fragments; D014760:Viral Fusion Proteins