title: Clinical evaluation of chemosensitivity testing for patients with unresectable non-small cell lung cancer (NSCLC) using collagen gel droplet embedded culture drug sensitivity test (CD-DST).
abstract: OBJECTIVE
  In the present study, we prospectively evaluated the clinical feasibility and efficacy of collagen gel droplet embedded culture drug sensitivity test (CD-DST) in unresectable non-small cell lung cancer (NSCLC) without previous treatment.
  
  
  METHODS
  Eighty patients with unresectable NSCLC, aged less than 81 years old, PS 0-1, and with evaluable tumor lesions, entered the study. If the patient had CD-DST active drugs, more than three cycles of chemotherapy containing these drugs were administered. If the patient did not have CD-DST active drugs, the patient could choose any treatment including best supportive care.
  
  
  RESULTS
  Of the 80 patients in this study, CD-DST yielded results successfully in 49 patients (61.3%). CD-DST active drugs were present in 22 patients, and significantly more female patients had in vitro active anticancer agents than male (P=0.0008). All of the patients with CD-DST active agents received chemotherapy including these agents. In these patients, the response rate was 72.7%, and median survival was 15.0 months. In the patients without CD-DST active agents, 11 patients received standard, empirical chemotherapy. In these patients, response rate was 0%, and median survival was 6.0 months.
  
  
  CONCLUSIONS
  The results show that CD-DST is capable of selecting the responders and the respective optimal regimens, and also delineating the patients less likely benefit from treatment.
authors.affiliation: Division of General Thoracic Surgery, Department of Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo, 160-8582, Japan. kawamura@sc.itc.keio.ac.jp
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kawamura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Gika
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Abiko
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Inoue
authors.forename: Takahiko
authors.initials: T
authors.lastname: Oyama
authors.forename: Yotaro
authors.initials: Y
authors.lastname: Izumi
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Koichi
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy*; D018929:Cell Culture Techniques / Q000379:methods*; D003094:Collagen / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005260:Female; D005782:Gels; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies
chemical_list: D005782:Gels; D003094:Collagen