title: Complete sequence of the large virulence plasmid pSFO157 of the sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- strain 3072/96.
abstract: The large virulence plasmid pSFO157 of sorbitol-fermenting E. coli O157:H(-) strain 3072/96 has a size of 121,239bp and contains 96 open reading frames >50bp. It is therefore 29,162bp larger (ca. 32%) than plasmid pO157 of E. coli O157:H7 strain EDL933. Major differences between the plasmids are the absence of katP, espP, and toxB in pSFO157 and, instead of these, the presence of the sfp fimbriae gene cluster and a large part of an F-plasmid transfer region, the latter accounting for most of the additional DNA. The differences in the order of the genes and their composition, as well as the presence of a number of replication-associated genes and mobile genetic elements suggests that the large E. coli O157 virulence plasmids have a complex evolutionary origin.
authors.affiliation: Institut für Hygiene und Mikrobiologie der Universität Würzburg, Josef-Schneider-Strasse 2, D-97070 Würzburg, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Brunder
authors.forename: Helge
authors.initials: H
authors.lastname: Karch
authors.forename: Herbert
authors.initials: H
authors.lastname: Schmidt
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003227:Conjugation, Genetic; D004261:DNA Replication; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D019453:Escherichia coli O157 / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D005144:F Factor / Q000235:genetics; D005285:Fermentation; D010861:Fimbriae, Bacterial / Q000235:genetics; D023061:Gene Order; D020071:Interspersed Repetitive Sequences; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013012:Sorbitol / Q000378:metabolism; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D013012:Sorbitol