In vitro evaluation of antagonistic properties of Pseudomonas corrugata.
Pseudomonas corrugata, a soil bacterium originally isolated from a temperate site of Indian Himalayan Region (IHR) is examined for its antagonistic activities against two phytopathogenic fungi, Alternaria alternata and Fusarium oxysporum. Although the bacterium did not show inhibition zones due to production of diffusible antifungal metabolites, a reduction in growth between 58% and 49% in both test fungi, A. alternata and F. oxysporum, was observed in sealed Petri plates after 120 h of incubation due to production of volatile antifungal metabolites. Reduction in biomass of A. alternata (93.8%) and F. oxysporum (76.9%) in Kings B broth was recorded after 48 h of incubation in dual culture. The antagonism was observed to be affected by growth medium, pH and temperature. The reduction in fungal biomass due to antagonism of bacteria was recorded maximum in the middle of the stationary phase after 21 h of inoculation. The production of siderophore, ammonia, lipase and chitinase in growth medium by P. corrugata were considered contributing to the antagonistic activities of the bacterium.
Trivedi
Pankaj
P
Environmental Physiology and Biotechnology, GB Pant Institute of Himalayan Environment and Development, Kosi-Katarmal-Almora, Uttaranchal, India.
Pandey
Anita
A
Palni
Lok Man S
LM
D000528:Alternaria / Q000187:drug effects / Q000254:growth & development*; D000898:Antibiosis; D000935:Antifungal Agents / Q000494:pharmacology; D018533:Biomass; D003470:Culture Media / Q000737:chemistry; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D007194:India; D011549:Pseudomonas / Q000302:isolation & purification / Q000502:physiology*; D012988:Soil Microbiology; D013696:Temperature; D013997:Time Factors
D000935:Antifungal Agents; D003470:Culture Media