Determination of GHB in urine and serum by LC/MS using a simple one-step derivative.
A sensitive and specific method for the determination of gamma-hydroxybutyric acid (GHB) in urine and serum is described. Prior to quantification by LC/MS in a Pauli-type ion trap, the molecule is converted by a fast and simple one-step procedure into its n-butyl ester derivative. Hexa-deutero GHB has been used as internal standard.
Kaufmann
Eckhard
E
Department of General Physiology, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, FRG, Germany. eckhard.kaufmann@uni-ulm.de
Alt
Andreas
A
D049429:Forensic Pathology / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006885:Hydroxybutyrates / Q000097:blood* / Q000652:urine*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D006885:Hydroxybutyrates; C111420:4-hydroxybutyric acid