title: Reorganization of mouse sperm lipid rafts by capacitation.
abstract: One of the hallmarks of mammalian sperm capacitation is the loss of cholesterol from the plasma membrane. Cholesterol has been associated with the formation of detergent insoluble membrane microdomains in many cell types, and sperm from several mammalian species have been shown to contain detergent-resistant membranes (DRMs). The change in cholesterol composition of the sperm plasma membrane during capacitation raises the question of whether the contents of DRMs are altered during this process. In this study, we investigated changes in protein composition of DRMs isolated from uncapacitated or capacitated mouse sperm. TX-100 insoluble membranes were fractionated by sucrose flotation gradient centrifugation and analyzed by Western and lectin blotting, and capacitation-related differences in protein composition were identified. Following capacitation, the detergent insoluble fractions moved to lighter positions on the sucrose gradients, reflecting a global change in density or composition. We identified several individual proteins that either became enriched or depleted in DRM fractions following capacitation. These data suggest that the physiological changes in sperm motility, ability to penetrate the zona pellucida (ZP), ZP responsiveness, and other capacitation-dependent changes, may be due in part to a functional reorganization of plasma membrane microdomains.
authors.affiliation: Department of Biology, University of Central Florida, Orlando, USA. cthaler@stanford.edu
authors.forename: Catherine D
authors.initials: CD
authors.lastname: Thaler
authors.forename: Monzy
authors.initials: M
authors.lastname: Thomas
authors.forename: Jenniffer R
authors.initials: JR
authors.lastname: Ramalie
mesh_terms: D000818:Animals; D003902:Detergents / Q000494:pharmacology; D037102:Lectins / Q000378:metabolism; D008297:Male; D008563:Membrane Lipids / Q000032:analysis; D021962:Membrane Microdomains / Q000737:chemistry* / Q000502:physiology; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D011975:Receptors, Mitogen / Q000378:metabolism; D013075:Sperm Capacitation / Q000502:physiology*; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: D003902:Detergents; D037102:Lectins; D008563:Membrane Lipids; D008565:Membrane Proteins; D011975:Receptors, Mitogen