title: Meganema perideroedes gen. nov., sp. nov., a filamentous alphaproteobacterium from activated sludge.
abstract: An industrial wastewater treatment plant at Grindsted, Denmark, has suffered from bulking problems for several years caused by filamentous bacteria. Five strains were isolated from the sludge by micromanipulation. Phylogenetic analysis of the 16S rRNA gene sequences showed that the strains formed a monophyletic cluster in the Alphaproteobacteria, and they were phenotypically different from their closest relatives and from all hitherto known filamentous bacteria described (closest relative Brevundimonas vesicularis ATCC 11426(T), 89.8 % sequence similarity). In pure culture, the cells (1.5-2.0 microm) in filaments are Gram-negative and contain polyphosphate and polyhydroxyalkanoates. The optimum temperature for growth is 30 degrees C and the strains grow in 2 % NaCl and are oxidase- and catalase-positive. Ubiquinone 10 is the major quinone. The major fatty acid (C(18 : 1)omega7c) and smaller amounts of unsaturated fatty acids, 3-hydroxy fatty acids with a chain length of 16 and 18 carbon atoms and small amounts of 10-methyl-branched fatty acids with 18 carbon atoms (C(19 : 0) 10-methyl) affiliated the strains with the Methylobacterium/Xanthobacter group in the Alphaproteobacteria. The G+C content of the DNA is 42.9 mol% (for strain Gr1(T)). The two most dissimilar isolates by 16S rRNA gene comparison (Gr1(T) and Gr10; 97.7 % identical) showed 71.5 % DNA-DNA relatedness. Oligonucleotide probes specific for the pure cultures were designed for fluorescence in situ hybridization and demonstrated that two filamentous morphotypes were present in the Grindsted wastewater treatment plant. It is proposed that the isolates represent a new genus and species, Meganema perideroedes gen. nov., sp. nov. The type strain of Meganema perideroedes is strain Gr1(T) (=DSM 15528(T)=ATCC BAA-740(T)).
authors.affiliation: Section of Environmental Engineering, Aalborg University, Sohngaardsholmsvej 57, DK-9000 Aalborg, Denmark.
authors.forename: Trine R
authors.initials: TR
authors.lastname: Thomsen
authors.affiliation: Advanced Wastewater Management Centre, School of Molecular and Microbial Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia.
authors.forename: Linda L
authors.initials: LL
authors.lastname: Blackall
authors.affiliation: Section of Environmental Engineering, Aalborg University, Sohngaardsholmsvej 57, DK-9000 Aalborg, Denmark.
authors.forename: Marilena Aquino
authors.initials: MA
authors.lastname: de Muro
authors.affiliation: Section of Environmental Engineering, Aalborg University, Sohngaardsholmsvej 57, DK-9000 Aalborg, Denmark.
authors.forename: Jeppe L
authors.initials: JL
authors.lastname: Nielsen
authors.affiliation: Section of Environmental Engineering, Aalborg University, Sohngaardsholmsvej 57, DK-9000 Aalborg, Denmark.
authors.forename: Per H
authors.initials: PH
authors.lastname: Nielsen
mesh_terms: D020561:Alphaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D001482:Base Composition; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D003718:Denmark; D005227:Fatty Acids / Q000032:analysis; D007220:Industrial Waste / Q000032:analysis; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012722:Sewage / Q000382:microbiology*; D012965:Sodium Chloride; D013045:Species Specificity; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D005227:Fatty Acids; D007220:Industrial Waste; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014451:Ubiquinone; D012965:Sodium Chloride