title: Identification of transmembrane domain of a membrane associated protein NS5 of Dendrolimus punctatus cytoplasmic polyhedrosis virus.
abstract: We examined the intracellular localization of NS5 protein of Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV) by expressing NS5-GFP fusion protein and proteins from deletion mutants of NS5 in baculovirus recombinant infected insect Spodoptera frugiperda (Sf-9) cells. It was found that the NS5 protein was present at the plasma membrane of the cells, and that the N-terminal portion of the protein played a key role in the localization. A transmembrane region was identified to be present in the N-terminal portion of the protein, and the detailed transmembrane domain (SQIHMVWVKSGLVFF, 57-71aa) of N-terminal portion of NS5 was further determined, which was accorded with the predicted results, these findings suggested that NS5 might have an important function in viral life cycle.
authors.affiliation: State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, P. R. China.
authors.forename: Wuguo
authors.initials: W
authors.lastname: Chen
authors.forename: Jiamin
authors.initials: J
authors.lastname: Zhang
authors.forename: Changjin
authors.initials: C
authors.lastname: Dong
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Li
authors.forename: Chuanfeng
authors.initials: C
authors.lastname: Liu
authors.forename: Yuanyang
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D002460:Cell Line; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins; D007304:Insect Viruses / Q000737:chemistry*; D016253:Microscopy, Immunoelectron; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry; D012087:Reoviridae / Q000737:chemistry*; D018411:Spodoptera / Q000166:cytology; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000737:chemistry*
chemical_list: D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; D049452:Green Fluorescent Proteins