title: [Western-blot with VLSE protein and "in vivo" antigens in Lyme borreliosis diagnosis].
abstract: The aim of the study was the evaluation of the efficiency of Western blot (EcoLine) test detecting simoultanous presence of IgM and IgG antibodies against B. burgdorferi in diagnosis of early and late stage of Lyme borreliosis. The comparison of results achieved by performing test Western-blot, ELISA (based on recombinant antigens of three genospecies of Borrelia) and EIA (based on antigens of one B. burgdorferi genospecies). The tests Western blot: EcoLine (Virotech) with antygens "in vivo", ELISA Borrelia IgM, IgG recombinant (Biomedica), EIA: B. b. ss. IgG, EIA B. garinii IgG, EIA B. afzelii IgG (TestLine) were used. Results showed efficacy of detecting IgM, IgG antibodies against VlsE simultanously and IgG antibodies against "in vivo" antigens in diagnosis of early stages of Lyme disease when atypical picture skin lessions arise diagnostic doubts and in discerning early and late stage of disease. The EIA tests based on one B. burgdoreferi genospecies seem less effective in comparison to ELISA tests based on 3 genospecies antigens.
authors.affiliation: Klinika Chorób Zakainych i Neuroinfekcji AM w Białymstoku.
authors.forename: Joanna
authors.initials: J
authors.lastname: Zajkowska
authors.forename: Maciej
authors.initials: M
authors.lastname: Kondrusik
authors.forename: Sławomir
authors.initials: S
authors.lastname: Pancewicz
authors.forename: Sambor
authors.initials: S
authors.lastname: Grygorczuk
authors.forename: Renata
authors.initials: R
authors.lastname: Swierzbińska
authors.forename: Teresa
authors.initials: T
authors.lastname: Hermanowska-Szpakowicz
authors.forename: Anna
authors.initials: A
authors.lastname: Czeczuga
authors.forename: Iwona
authors.initials: I
authors.lastname: Sienkiewicz
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D025065:Borrelia burgdorferi / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D015787:Erythema Chronicum Migrans / Q000175:diagnosis; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008074:Lipoproteins / Q000378:metabolism*; D008193:Lyme Disease / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D020852:Lyme Neuroborreliosis / Q000175:diagnosis; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008074:Lipoproteins; C105546:VlsE protein, Borrelia burgdorferi