Expression and GTP sensitivity of peptide histidine isoleucine high-affinity-binding sites in rat.
High-affinity-binding sites for the vasoactive intestinal peptide (VIP) analogs peptide histidine/isoleucine-amide (PHI)/carboxyterminal methionine instead of isoleucine (PHM) are expressed in numerous tissues in the body but the nature of their receptors remains to be elucidated. The data presented indicate that PHI discriminated a high-affinity guanosine 5'-triphosphate (GTP)-insensitive-binding subtype that represented the totality of the PHI-binding sites in newborn rat tissues but was differentially expressed in adult animals. The GTP-insensitive PHI/PHM-binding sites were also observed in CHO cells over expressing the VPAC2 but not the VPAC1 VIP receptor.
Debaigt
Colin
C
IPBC CNRS-UMR 6187 Pôle Biologie Santé, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
Meunier
Annie-Claire
AC
Goursaud
Stephanie
S
Montoni
Alicia
A
Pineau
Nicolas
N
Couvineau
Alain
A
Laburthe
Marc
M
Muller
Jean-Marc
JM
Janet
Thierry
T
D000818:Animals; D001665:Binding Sites; D016466:CHO Cells; D006224:Cricetinae; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D010451:Peptide PHI / Q000378:metabolism* / Q000494:pharmacology*; D051381:Rats; D051239:Receptors, Vasoactive Intestinal Peptide, Type II / Q000235:genetics / Q000378:metabolism; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity
D010451:Peptide PHI; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; D011994:Recombinant Proteins; D006160:Guanosine Triphosphate