[Optimization of SRAP & ISSR technology and its application in the identification of seeds of Brassica oleracea L].
In this study, the molecular marker technology of SRAP and ISSR were applied in rapid identification of seeds from eight species of Brassica oleracea L. Firstly, using the genomic DNA of cabbage as template, SRAP and ISSR reaction systems were optimized through testing every factor, respectively, that affects PCR amplification. Then, using the optimized reaction systems, 30 SRAP primer pairs and 15 ISSR primers were applied to amplify genomic DNA of cabbage, savoy, purple cabbage, borecole, cauliflower, broccoli, Brussels sprouts, and kohlrabi The results showed that high polymorphisms were exhibited among the eight species of Brassica oleracea L. by SRAP primer pairs of M3-E5 and M4-E5, as well as ISSR primers of 844 and 888, especially primer 844 which can identify all eight materials efficiently.
Liu
Chong
C
Horticultural Research Institute, SAAS, Shanghai Key Lab of Protected Horticultural Technology, Shanghai 201106, China.
Ge
Cai-Lin
CL
Ren
Yun-Ying
YY
Chen
Jin-Xiu
JX
Yang
Xiao-Feng
XF
Bo
Tian-Yue
TY
D001937:Brassica / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012639:Seeds / Q000235:genetics*