Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae.
We developed a system that allows the selection of the reciprocal products resulting from spontaneous mitotic cross-overs in the yeast Saccharomyces cerevisiae. A number of other types of genetic events, including chromosome loss, can be monitored with this system. For a 120-kb chromosome interval on chromosome V (CEN5-CAN1), the rate of mitotic cross-overs was 4x10(-5) per division, a rate approximately 25,000-fold lower than the meiotic rate of cross-overs. We found no suppression of mitotic cross-overs near the centromere of chromosome V, unlike the suppression observed for meiotic exchanges. The rate of reciprocal cross-overs was substantially (38-fold) elevated by treatment of cells with hydroxyurea, a drug that reduces nucleotide pools and slows DNA replication.
Barbera
Maria A
MA
Department of Molecular Genetics and Microbiology, Box 3054, Duke University Medical Center, Durham, NC 27710, USA.
Petes
Thomas D
TD
D002503:Centromere / Q000235:genetics; D002872:Chromosome Deletion; D015825:Chromosomes, Fungal / Q000235:genetics; D003434:Crossing Over, Genetic / Q000187:drug effects / Q000235:genetics; D006579:Heterozygote; D006918:Hydroxyurea / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000235:genetics*; D012641:Selection, Genetic
D006918:Hydroxyurea