title: Patterns of genetic diversity and differentiation in the tsetse fly Glossina morsitans morsitans Westwood populations in East and southern Africa.
abstract: Genetic diversity and differentiation within and among nine G. morsitans morsitans populations from East and southern Africa was assessed by examining variation at seven microsatellite loci and a mitochondrial locus, cytochrome oxidase (COI). Mean COI diversity within populations was 0.63+/-0.33 and 0.81 taken over all populations. Diversities averaged over microsatellite loci were high (mean number of alleles/locus>or=7.4; mean HE>or=65%) in all populations. Diversities averaged across populations were greater in East Africa (mean number of alleles=22+/-2.6; mean he=0.773+/-0.033) than in southern Africa (mean number of alleles=18.7+/-4.0; mean he=0.713+/-0.072). Differentiation among all populations was highly significant (RST=0.25, FST=0.132). Nei's Gij statistics were 0.09 and 0.19 within regions for microsatellites and mitochondria, respectively; between regions, Gij was 0.14 for microsatellites and 0.23 for mitochondria. GST among populations was 0.23 for microsatellite loci and 0.40 for mitochondria. The F, G and R statistics indicate highly restricted gene flow among G. m. morsitans populations separated over geographic scales of 12-917 km.
authors.affiliation: Department of Entomology, Iowa State University, Ames, Iowa 50011-3222, USA.
authors.forename: J O
authors.initials: JO
authors.lastname: Ouma
authors.forename: J G
authors.initials: JG
authors.lastname: Marquez
authors.forename: E S
authors.initials: ES
authors.lastname: Krafsur
mesh_terms: D000351:Africa, Eastern; D000353:Africa, Southern; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D051456:Gene Flow; D017344:Genes, Insect; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008297:Male; D018895:Microsatellite Repeats; D014370:Tsetse Flies / Q000201:enzymology / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV