[Expression of multi-copy and immuno-reactivity of recombinant type-specific epitope of herpes simplex virus type 1].
OBJECTIVE
To express a multi-copy specific epitope recombinant protein of herpes simplex virus type 1 (HSV-1) with immuno-reactivity.


METHODS
Multi-copy genes with a specific epitope of HSV-1-glycoprotein G 112-127 were constructed by DNA recombination and cloned in E. coli JM109 pGEM-5Zf. The positive recombinants were determined by SDS-PAGE and Western blotting.


RESULTS
The recombinants with 4, 8, 16 and 32 copies of gG 112-127 were obtained. The 8-copy recombinant was expressed by 17.5%, mainly as inclusion body. And it reacted with antiserum HSV-1, but not with antiserum HSV-2.


CONCLUSIONS
The HSV-1-gG112-127 recombinant could be used to distinguish HSV-1 and HSV-2 in ELISA.
Ji
Xiao-wei
XW
Department of Clinical Microbiology and Immunology, the Third Military Medical University and Chongqing Research Center of Biopharmaceutical Engineering Technology, Chongqing 400038, China.
Mao
Xu-hu
XH
Zou
Quan-ming
QM
Yu
Qing-tan
QT
Zhao
Li-li
LL
D000937:Antigen-Antibody Reactions; D004274:DNA, Recombinant; D000939:Epitopes / Q000096:biosynthesis* / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000276:immunology
D004274:DNA, Recombinant; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins