[Purification and properties of isoflavone-glucosidase].
A high activity isoflavone-glucosidase, which hydrolysis glycosides, was obtainde using liquid fermentation from Absidia sp. R strain. The isoflavone-glucosidase was purified 11 folds with yielding rate of 10.9% after ammonium sulfate precipitation and DEAE-Cellocuse (DE-52) ion exchange chromatography. SDS-PAGE results showed that the molecular weight is 53kD. And the optimum temperature, the optimum pH, Km and pI of the enzyme are 50 deegrees C, 5.0, 1.3 x 10(-2) mol/L and 3.2, respectively. The isoflavone-glucosidase is also rather stable under 60 degrees C and in pH range from 5.0 to 7.0. The enzyme can be activated by Co2+ and Ca2+, and be inhibited by Ag+ and Cu2+.
Xie
Ming-Jie
MJ
College of Life Science, Liaoning Normal University, Dalian 116029, China. xmj1222@sina.com
Song
Ming
M
Zou
Cui-Xia
CX
Xu
Chun-Hua
CH
Lu
Ming-Chun
MC
Jin
Feng-Xie
FX
D020095:Absidia / Q000201:enzymology*; D005959:Glucosidases / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000378:metabolism*; D013696:Temperature
D007529:Isoflavones; D005959:Glucosidases