Expression of human immunodeficiency virus type 1 tat from a replication-deficient herpes simplex type 1 vector induces antigen-specific T cell responses.
Herpes simplex type-1 virus (HSV-1) based vectors have been widely used in different gene therapy approaches and also as experimental vaccines against HSV-1 infection. Recent advances in the HSV-1 technology do support the use of replication defective HSV-1 as vaccine vectors for delivery of foreign antigens. We have examined the ability of a recombinant replication-defective HSV-1 vector expressing the HIV-1 Tat protein to induce long-term Tat-specific immune responses in the Balb/c murine model. The results showed that vector administration by the subcutaneous route elicits anti-Tat specific T-cell mediated immune responses in mice characterized by the presence of the Tat-specific cytotoxic activity and production of high levels of IFN-gamma.
Bozac
Aleksandra
A
University of Ferrara, Department of Experimental and Diagnostic Medicine, Section of Microbiology, Via Luigi Borsari 46, 44100 Ferrara, Italy.
Berto
Elena
E
Vasquez
Federica
F
Grandi
Paola
P
Caputo
Antonella
A
Manservigi
Roberto
R
Ensoli
Barbara
B
Marconi
Peggy
P
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000281:Administration, Intranasal; D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D015696:Gene Products, tat / Q000096:biosynthesis* / Q000276:immunology*; D005822:Genetic Vectors; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000276:immunology*; D007115:Immunization Schedule; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D014709:Vero Cells; D014779:Virus Replication / Q000235:genetics*
D016915:AIDS Vaccines; D015696:Gene Products, tat; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; D007371:Interferon-gamma