title: The NADPH- and iron-dependent lipid peroxidation in human placental microsomes.
abstract: In pregnant females, placenta is the most important source of lipid hydroperoxides and other reactive oxygen species (ROS). The increased production of lipid peroxides is often linked to preeclampsia. In our study, we revealed that NADPH- and iron-dependent lipid peroxidation in human placental microsomes (HPM) occurred. In the presence of Fe2+ ion, HPM produced small amounts of thiobarbituric acid-reactive substances (TBARS) - a final product of lipid peroxidation. NADPH caused a strong increase of iron stimulated TBARS formation. TBARS formation was inhibited by superoxide dismutase, butylated hydroxytoluene and alpha-tocopherol but not by mannitol or catalase. TBARS and superoxide radical production was inhibited in similar manner by cytochrome P450 inhibitors. The results obtained led us to the following conclusions: (1) microsomal lipid peroxidation next to mitochondrial lipid peroxidation may by an important source of lipid hydroperoxides in blood during pregnancy and (2) superoxide radical released by microsomal cytochrome P450 is an important factor in NADPH- and iron-dependent lipid peroxidation in HPM.
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University of Gdańsk, ul. Debinki 1, 80-211, Gdańsk, Poland. rysmil@amg.gda.pl
authors.forename: Ryszard
authors.initials: R
authors.lastname: Milczarek
authors.forename: Ewa
authors.initials: E
authors.lastname: Sokolowska
authors.forename: Anna
authors.initials: A
authors.lastname: Hallmann
authors.forename: Jerzy
authors.initials: J
authors.lastname: Klimek
mesh_terms: D000323:Adrenochrome / Q000096:biosynthesis; D000818:Animals; D002417:Cattle; D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D007501:Iron / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008861:Microsomes / Q000187:drug effects* / Q000201:enzymology; D009249:NADP / Q000494:pharmacology*; D010920:Placenta / Q000187:drug effects* / Q000201:enzymology; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
chemical_list: D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D009249:NADP; D000323:Adrenochrome; D007501:Iron; D013482:Superoxide Dismutase