Isolation and characterization of human embryonic stem cells.
The derivation of human embryonic stem (hES) cells is a challenging procedure. The isolation and maintenance of hES is visually and manually complicated, involving mechanical or enzymatic passaging using either collagenase or trypsin. This chapter describes detailed protocols that have been used for the derivation, maintenance, and characterization of hES cells in vitro along with protocols to test their differentiation potential in vivo. When used as a guideline, these protocols will assist researchers in setting up a hES cell laboratory.
Mitalipova
Maisam
M
Department of Animal and Dairy Sciences, University of Georgia, Athens, USA.
Palmarini
Gail
G
D015415:Biomarkers; D018929:Cell Culture Techniques / Q000379:methods* / Q000592:standards; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods* / Q000592:standards; D018920:Coculture Techniques / Q000379:methods; D015925:Cryopreservation; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D007753:Laboratories; D039904:Pluripotent Stem Cells / Q000166:cytology*; D011786:Quality Control
D015415:Biomarkers