Adherence of Staphylococcus epidermidis to biomaterials is augmented by PIA.
Staphylococcus epidermidis is the most common cause of orthopaedic prosthetic device infections. Polysaccharide intercellular adhesin (PIA) is important in the pathogenesis of intravascular catheter-associated infection, and has an essential role in cellular aggregation and biofilm formation. However, the role of PIA in orthopaedic infections is less well understood. We used genetically defined strains of S. epidermidis in an in vitro adherence assay to assess the importance of PIA in the adherence to various orthopaedic biomaterials. On all biomaterials tested (zirconia, ultra-high molecular weight polyethylene, polymethylmethacrylate, cobalt chromium, titanium, stainless steel, and silastic), PIA-positive S. epidermidis 1457 exhibited greater levels of adherence thanS. epidermidis 1457 M10, an isogenic icaA Tn917 mutant. PIA appears to play a critical role in the adherence of S. epidermidis to orthopaedic biomaterials, and may serve as an important virulence determinant in orthopaedic prosthetic device infections.
Olson
M E
ME
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.
Garvin
K L
KL
Fey
P D
PD
Rupp
M E
ME
D001422:Bacterial Adhesion / Q000502:physiology*; D001672:Biocompatible Materials; D002858:Chromium Alloys; D004129:Dimethylpolysiloxanes; D066298:In Vitro Techniques; D020959:Polyethylene; D019904:Polymethyl Methacrylate; D011135:Polysaccharides, Bacterial / Q000502:physiology*; D019736:Prostheses and Implants; D012828:Silicones; D013193:Stainless Steel; D013212:Staphylococcus epidermidis / Q000502:physiology*; D014025:Titanium; D015040:Zirconium
D001672:Biocompatible Materials; D002858:Chromium Alloys; D004129:Dimethylpolysiloxanes; D011135:Polysaccharides, Bacterial; D012828:Silicones; C113579:polysaccharide intercellular adhesin; D013193:Stainless Steel; C013830:baysilon; D020959:Polyethylene; D019904:Polymethyl Methacrylate; D015040:Zirconium; D014025:Titanium; C028541:zirconium oxide