Multilocus enzyme electrophoresis and cytochrome B gene sequencing-based identification of Leishmania isolates from different foci of cutaneous leishmaniasis in Pakistan.
Seventeen Leishmania stocks isolated from cutaneous lesions of Pakistani patients were studied by multilocus enzyme electrophoresis and by polymerase chain reaction amplification and sequencing of the cytochrome b (Cyt b) gene. Eleven stocks that expressed nine zymodemes were assigned to L. (Leishmania) major. All of them were isolated from patients in the lowlands of Larkana district and Sibi city in Sindh and Balochistan provinces, respectively. The remaining six, distributed in two zymodemes (five and one), isolated from the highland of Quetta city, Balochistan, were identified as L. (L.) tropica. The same result at species level was obtained by the Cyt b sequencing for all the stocks examined. No clear-cut association between the clinical features (wet or dry type lesions) and the Leishmania species involved was found. Leishmania (L.) major was highly polymorphic compared with L. (L.) tropica. This difference may be explained by the fact that humans may act as a sole reservoir of L. (L.) tropica in anthroponotic cycles; however, many wild mammals can be reservoirs of L. (L.) major in zoonotic cycles.
Marco
Jorge D
JD
Department of Parasitology, Kochi Medical School, Kochi University, Kohasu, Kochi, Japan. macrojd@med.kochi-ms.ac.jp
Bhutto
Abdul M
AM
Soomro
Farooq R
FR
Baloch
Javed H
JH
Barroso
Paola A
PA
Kato
Hirotomo
H
Uezato
Hiroshi
H
Katakura
Ken
K
Korenaga
Masataka
M
Nonaka
Shigeo
S
Hashiguchi
Yoshihisa
Y
D000818:Animals; D045303:Cytochromes b / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry; D004311:Double-Blind Method; D004586:Electrophoresis / Q000379:methods / Q000592:standards; D004798:Enzymes / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D010154:Pakistan; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods
D017931:DNA Primers; D016054:DNA, Protozoan; D004798:Enzymes; D045303:Cytochromes b