title: Influence of the methodology and evaluation criteria on determining microleakage in dentin-restorative interfaces.
abstract: This in vitro study compared microleakage along the dentin-restorative interface using a spectrophotometer protocol and two conventional single-surface methods (scores and percentages), using an organic dye (0.5% buffered methylene blue) or a tracer (50% silver nitrate). Occluso-proximal preparations with gingival margins in dentin were made in 40 human teeth. The teeth were divided into four groups (n=10) according to the solution dyes and adhesive system used: group 1, single bond/methylene blue; group 2, single bond/silver nitrate; group 3, Clearfil SE Bond/methylene blue; and group 4, Clearfil SE Bond/silver nitrate. The dye penetration measurements were made in all groups, using scores and percentages. Groups 1 and 3 were also assessed by UV spectrophotometer. For percentage measurement, the data were submitted to ANOVA and Tukey's test. For the material factor, there was a statistically significant difference between groups 1 and 3. For the dye factor, there was a statistically significant difference between groups 3 and 4. The score results were submitted to Kruskall-Wallis test and showed differences between groups 1 and 3 and groups 2 and 3. For spectrophotometer measurement, no significant difference was observed between groups 1 and 3. The results of dye penetration suggest that there was a difference between dyes and measurement methods, and this should change the interpretation of microleakage tests.
authors.affiliation: Department of Restorative Dentistry, Vale do Itajaí University-UNIVALI, Itajaí, Brazil.
authors.forename: Denise Arliane
authors.initials: DA
authors.lastname: Amarante de Camargo
authors.forename: Mário Alexandre Coelho
authors.initials: MA
authors.lastname: Sinhoreti
authors.forename: Lourenço
authors.initials: L
authors.lastname: Correr-Sobrinho
authors.forename: Manoel Damião
authors.initials: MD
authors.lastname: de Sousa Neto
authors.forename: Simonides
authors.initials: S
authors.lastname: Consani
mesh_terms: D000704:Analysis of Variance; D004396:Coloring Agents; D003763:Dental Leakage / Q000175:diagnosis*; D006801:Humans; D008751:Methylene Blue; D012835:Silver Nitrate; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D018709:Statistics, Nonparametric
chemical_list: D004396:Coloring Agents; D012835:Silver Nitrate; D008751:Methylene Blue