title: Gordonia araii sp. nov. and Gordonia effusa sp. nov., isolated from patients in Japan.
abstract: Two bacterial strains, IFM 10211(T) and IFM 10200(T), were isolated from the sputum of two Japanese patients, and were subjected to a polyphasic taxonomic study. The two strains were found to have morphological, physiological and chemotaxonomic properties that were consistent with their assignment to the genus Gordonia, except for a few chemotaxonomic characteristics. Almost complete 16S rRNA gene sequences of the two strains were determined; the data showed that they are related distantly to Gordonia amarae, Gordonia hirsuta, Gordonia hydrophobica and Gordonia sihwensis, showing 16S rRNA gene sequence similarities to the type strains of these species of 96.2-97.9 %. DNA-DNA relatedness data coupled with the combination of genotypic and phenotypic data indicated that the two strains are representatives of two novel, separate species. The names proposed to accommodate these two strains are Gordonia araii sp. nov. (type strain IFM 10211(T)=DSM 44811(T)=NBRC 100433(T)=JCM 12131(T)) and Gordonia effusa sp. nov. (type strain IFM 10200(T)=DSM 44810(T)=NBRC 100432(T)=JCM 12130(T)).
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kageyama
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Soji
authors.initials: S
authors.lastname: Iida
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Katsukiyo
authors.initials: K
authors.lastname: Yazawa
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Kudo
authors.affiliation: Discovery Research Laboratories, Tanabe Seiyaku Co. Ltd, 2-2-50 Kawagishi, Toda, Saitama 335-8505, Japan.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Suzuki
authors.affiliation: First Department of Internal Medicine, Kurume University School of Medicine, 67 Asahimachi, Kurume 830-0011, Japan.
authors.forename: Takeharu
authors.initials: T
authors.lastname: Koga
authors.affiliation: Laboratory of Clinical Microbiology, Toranomon Hospital, Toranomon 2-2-2, Minato-ku, Tokyo 105-8470, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Saito
authors.affiliation: Laboratory of Clinical Microbiology, Toranomon Hospital, Toranomon 2-2-2, Minato-ku, Tokyo 105-8470, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Inagawa
authors.affiliation: National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Akihito
authors.initials: A
authors.lastname: Wada
authors.affiliation: Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
authors.forename: Reiner M
authors.initials: RM
authors.lastname: Kroppenstedt
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Mikami
mesh_terms: D000368:Aged; D001482:Base Composition; D003352:Corynebacterium / Q000737:chemistry / Q000145:classification* / Q000502:physiology; D003354:Corynebacterium Infections / Q000150:complications / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007564:Japan; D007674:Kidney Diseases / Q000150:complications; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010641:Phenotype; D018410:Pneumonia, Bacterial / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013183:Sputum / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S