[The effects of flutamide on the full gene expression of early testis development of embryonic period male SD].
OBJECTIVE
To study the effects of Flutamide (Flu) on the full gene expression of early testis development of embryonic period in male SD rats.


METHODS
Flu was injected subcutaneously (sc) with NS at doses of 6.25 mg/kg x d from 12th to 17th gestation days (GD), and in control group with tales doses NS. Fifty pregnant SD rats were sacrificed on GD 20. The mRNA were extracted respectively from fetus testis with treated and untreated Flu, then transcribed reversely to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently, cDNA probes were hybridized to the Mouse40S cDNA microarray and fluorescence signals were analyzed. Meanwhile, we took out of the right testes of male offspring to make the detection of immunohistochemistry (IHC). Furthermore, we determined the level of testosterone by enzyme-linked immunosorbentassay (ELISA).


RESULTS
Compared with the control group, a total of 31 identified genes expressed differently, including 14 down-regulated and 17 upregulated genes. However, the function of 5 down-regulated and 10 upregulated difference expression genes are unknown in Genbank. This finding has provided important reference basis for the molecular mechanism of reproductive development of Flu in the future. The activity of two important enzymes was cut down by IHC. The level of testosterone was decreased by ELISA.


CONCLUSIONS
The results indicated that Flu administrated to F0 pregnant rats can damage apparently to the reproductive development of embryonic period male SD rats in many ways, and can cause the abnormality of sexual differentiation and sexual development in rats.
Li
Yan
Y
Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Zhang
Hao
H
Liang
Ying
Y
Wu
De-sheng
DS
D000726:Androgen Antagonists / Q000633:toxicity*; D000818:Animals; D005260:Female; D005485:Flutamide / Q000633:toxicity*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012741:Sexual Maturation / Q000187:drug effects*; D013737:Testis / Q000196:embryology*
D000726:Androgen Antagonists; D005485:Flutamide