Construction of a cultivation system of a yeast single cell in a cell chip microchamber.
A novel single cell screening system was constructed using a yeast cell chip in combination with the yeast cell surface engineering [NanoBiotechnology 2005, 1, 105-111]. Enzymes or functional proteins displayed on a yeast cell surface can be used as a protein cluster. To achieve high-throughput screening of protein libraries on the cell surface, a catalytic reaction by a single cell-surface-engineered yeast cell was successfully carried out in the microchamber on the yeast cell chip. After screening, to replicate a target cell for use in measuring of activity, DNA sequencing, and preservation, a novel single cell cultivation system in the yeast cell chip was constructed. To avoid damage of the rapid dry up of medium in the microchamber array, the yeast cell chip was modified with a protection sheet, so that the modified chip was like a micro-culture tank constructed on the yeast cell chip microchamber. As a result, single yeast cell cultivation in the yeast cell chip microchamber was observed, and the modified yeast cell chip was evaluated to be good for a single cell selection. The improvement showed that the single cell screening system coupled with the single cell cultivation using the modified yeast cell chip may be superior to that by a cell sorter for the isolation of a target cell and its practical use.
Fukuda
Takeshi
T
Fiber Amenity Engineering, Graduate School of Engineering, University of Fukui, Japan.
Shiraga
Seizaburo
S
Kato
Michiko
M
Suye
Shin-Ichiro
S
Ueda
Mitsuyoshi
M
D002452:Cell Count; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002469:Cell Separation; D003470:Culture Media; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods*; D008856:Microscopy, Fluorescence; D009172:Mycology / Q000295:instrumentation* / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins