The bifunctional glyceryl transferase/phosphatase OzmB belonging to the HAD superfamily that diverts 1,3-bisphosphoglycerate into polyketide biosynthesis.
The HAD superfamily protein OzmB from the oxazolomycin biosynthetic pathway is shown to divert the primary metabolite 1,3-diphosphoglycerate into the polyketide biosynthetic pathway as glycerate via loading of a carrier protein. Each of the steps-activation of d-3-phosphoglycerate, dephosphorylation while attached to a cysteine on OzmB, and subsequent transfer of glycerate to the phosphopantetheinyl thiol of an acyl carrier protein-was monitored by nanospray Fourier transform mass spectrometry. This activation of phosphoglycerate represents a general mechanism of diverting glycolytic metabolites into glyceryl-derived polyketides.
Dorrestein
Pieter C
PC
Department of Chemistry, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
Van Lanen
Steven G
SG
Li
Wenli
W
Zhao
Chunhua
C
Deng
Zixin
Z
Shen
Ben
B
Kelleher
Neil L
NL
D000595:Amino Acid Sequence; D004163:Diphosphoglyceric Acids / Q000737:chemistry*; D004789:Enzyme Activation; D018942:Macrolides / Q000138:chemical synthesis / Q000737:chemistry*; D008780:Methyltransferases / Q000138:chemical synthesis / Q000737:chemistry; D008969:Molecular Sequence Data; D010080:Oxazoles / Q000138:chemical synthesis / Q000737:chemistry*; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry*; D011714:Pyrans / Q000138:chemical synthesis / Q000737:chemistry; D011760:Pyrrolidinones; D016415:Sequence Alignment; D013141:Spiro Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D013379:Substrate Specificity; D014166:Transferases / Q000737:chemistry*
D004163:Diphosphoglyceric Acids; D018942:Macrolides; D010080:Oxazoles; D011714:Pyrans; D011760:Pyrrolidinones; D013141:Spiro Compounds; C053079:tautomycin; C077919:diffusomycin; C015891:glycerate 1,3-biphosphate; D014166:Transferases; C082704:FK520 methyltransferase; D008780:Methyltransferases; C042308:phosphatidylglycerol glyceryl transferase; D010744:Phosphoric Monoester Hydrolases