title: A DNA prime-oral Listeria boost vaccine in rhesus macaques induces a SIV-specific CD8 T cell mucosal response characterized by high levels of alpha4beta7 integrin and an effector memory phenotype.
abstract: In this study in Rhesus macaques, we tested whether IL-12 or IL-15 in a DNA prime-oral Listeria boost amplifies the SIV-Gag-specific CD8 mucosal response. SIV-specific CD8 T cells were demonstrated in the peripheral blood (PB) in all test vaccine groups, but not the control group. SIV-Gag-specific CD8 T cells in the PB expressed alpha4beta7 integrin, the gut-homing receptor; a minor subset co-express alphaEbeta7 integrin. SIV-Gag-specific CD8 T cells were also detected in the gut tissue, intraepithelial (IEL) and lamina propria lymphocytes (LPL) of the duodenum and ileum. These cells were characterized by high levels of beta7 integrin expression and a predominance of the effector memory phenotype. Neither Il-12 nor IL-15 amplified the frequency of SIV-specific CD8 T cells in the gut. Thus, the DNA prime-oral Listeria boost strategy induced a mucosal SIV-Gag-specific CD8 T cell response characterized by expression of the alpha4beta7 integrin gut-homing receptor.
authors.affiliation: Microbiology Department, University of Pennsylvania, 323 Johnson Building, 3610 Hamilton Walk, Philadelphia, PA 19104, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Neeson
authors.forename: Jean
authors.initials: J
authors.lastname: Boyer
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Kumar
authors.forename: Mark G
authors.initials: MG
authors.lastname: Lewis
authors.forename: Lennox
authors.initials: L
authors.lastname: Mattias
authors.forename: Ron
authors.initials: R
authors.lastname: Veazey
authors.forename: David
authors.initials: D
authors.lastname: Weiner
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Paterson
mesh_terms: D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004064:Digestive System / Q000276:immunology; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018928:Immunity, Mucosal; D007117:Immunization, Secondary; D007156:Immunologic Memory; D016023:Integrins / Q000378:metabolism*; D008087:Listeria / Q000235:genetics* / Q000276:immunology; D008253:Macaca mulatta; D009092:Mucous Membrane; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D001428:Bacterial Vaccines; D015683:Gene Products, gag; D016023:Integrins; D019444:Vaccines, DNA; C070709:integrin alphaEbeta7