[Wilms tumor as a novel model of angiogenesis in childood nephropathies].
Wilms tumor has a unique possibility of recapitulation within its substance different stages of renal development. Vascular endothelial growth factor (VEGF) and its receptors (VEGFR-1 and VEGFR-2) are regarded to play the crucial role in the process of simultaneous development of tubules and glomeruli in animal kidney. Neoangiogenesis, secondary to rearrangement of epithelial elements in Wilms tumor, may therefore follow the lack of glomeruli in this neoplasm. The aim of the present research was an immunohistochemical analysis of VEGF-C and VEGFR-2 expressions in Wilms tumor and an attempt of explanation of neovascularisation process in this malignancy. The study group was composed of 16 children diagnosed with Wilms tumor (stage III of clinical classification) hospitalised in Department of Paediatric Oncology, Hematology and Transplantology, University of Medical Sciences in Poznan. The indirect immunohistochemical assay with the use of monoclonal antibodies directed against VEGF-C and VEGFR-2 was employed. VEGF-C expression was detected within blastemal and hypocellular stromal components of Wilms tumor. On the other hand, immuno-reactivity of VEGFR-2 was established in dysplastic tubules in the closest proximity of VEGF-C positive parts of stromal origin. VEGF-C dependent neovascularisation in Wilms tumor may follow an adequate differentiation of already existing epithelial elements. It may also explain the process of glomeruli-dependent physiological development of kidney and, what is also probable, the phenomenon of neoangiogenesis described in individual childhood nephropathies.
Nowicki
Michał
M
Katedra Histologii i Embriologii, Akademii Medycznej w Poznaniu. mnowicki@amp.edu.pl
Ostalska-Nowicka
Danuta
D
Zachwieja
Jacek
J
Lewandowska
Lidia
L
Kaczmarek-Kanold
Małgorzata
M
Miśkowiak
Bogdan
B
D002648:Child; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000648:ultrastructure*; D008954:Models, Biological; D009389:Neovascularization, Pathologic / Q000503:physiopathology*; D040262:Receptors, Vascular Endothelial Growth Factor / Q000378:metabolism; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism; D009396:Wilms Tumor / Q000378:metabolism* / Q000648:ultrastructure*
D042442:Vascular Endothelial Growth Factors; D040262:Receptors, Vascular Endothelial Growth Factor