Expression of CD44 adhesion molecule in rat testis with ischemia/reperfusion injury.
The expression pattern of CD44 was studied in the rat testis following ischemia/reperfusion (I/R) injury to elucidate the possible role of the CD44 adhesion molecule in acute experimental testicular torsion. Western blot analysis showed that CD44 expression began to increase significantly 24 hr after reperfusion, compared with the normal control; the increased expression persisted until 96 hr after I/R. Immunohistochemistry showed that, in the normal testis, CD44 was constitutively expressed mainly in ED2-positive resident macrophages in the interstitial space. After I/R, the majority of inflammatory cells in the interstitial space surrounding the damaged tubules were ED1-positive macrophages that were CD44-positive. These findings suggest that the significant increase in CD44 expression that occurs during the delayed phase after reperfusion originates from infiltrating macrophages possibly in anticipation of the migration and adhesion of additional macrophages into the affected testis.
Moon
Changjong
C
Department of Veterinary Medicine, College of Applied Life Science and Applied Radiological Science Research Institute, Cheju National University, Jeju, Republic of Korea.
Jeong
Chan-woo
CW
Kim
Heechul
H
Ahn
Meejung
M
Kim
Seungjoon
S
Shin
Taekyun
T
D000818:Animals; D018960:Hyaluronan Receptors / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*; D013737:Testis / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology
D018960:Hyaluronan Receptors