Thiorhodococcus mannitoliphagus sp. nov., a purple sulfur bacterium from the White Sea.
A novel purple sulfur bacterium, strain WS(T), was isolated from a microbial mat from an estuary of the White Sea. Individual cells are coccoid shaped, motile by flagella and do not contain gas vesicles. The mean cell diameter is 1.85 mum (range 1.5-2.0 mum). Cell suspensions exhibit a purple-violet colour. They contain bacteriochlorophyll a and carotenoids of the rhodopinal series as photosynthetic pigments. The novel bacterium is an anoxygenic photoautotroph, using sulfide, thiosulfate, sulfite and elemental sulfur as electron donors for photosynthesis and is capable of photoassimilating several organic carbon sources in the presence of carbonate and a reduced sulfur source (sulfide and/or thiosulfate). Sulfur globules, formed during oxidation of sulfide, are stored transiently inside the cells. Optimal salinity and pH for growth are at 0.5-2.0 % NaCl and pH 7.0-7.5. The DNA base composition of strain WS(T) is 61.8 mol% G+C. 16S rRNA gene sequence analysis showed that the new isolate belongs to the genus Thiorhodococcus, with Thiorhodococcus minor CE2203(T) as the nearest relative (sequence similarity of 97.3 %). Several distinct differences from described species necessitate the description of a novel species. Thiorhodococcus mannitoliphagus sp. nov. is the proposed name, with strain WS(T) (=ATCC BAA-1228(T)=VKM B-2393(T)) as the type strain.
Rabold
Sandra
S
Leibniz Institut für Meereswissenschaften IFM-GEOMAR, Düsternbrooker Weg 20, D-24105 Kiel, Germany.
Gorlenko
Vladimir M
VM
Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia.
Imhoff
Johannes F
JF
Leibniz Institut für Meereswissenschaften IFM-GEOMAR, Düsternbrooker Weg 20, D-24105 Kiel, Germany.
D001429:Bacteriochlorophylls / Q000032:analysis; D001482:Base Composition; D002338:Carotenoids / Q000032:analysis; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D020563:Gammaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride; D013045:Species Specificity; D013440:Sulfides / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D014871:Water Microbiology
D001429:Bacteriochlorophylls; D003470:Culture Media; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D002338:Carotenoids; D012965:Sodium Chloride; D013455:Sulfur