Enantioselective reduction of acetophenone and its derivatives with a new yeast isolate Candida tropicalis PBR-2 MTCC 5158.
The enantioselective bioreduction of acetophenone and its various analogues has been carried out using a new yeast strain, Candida tropicalis MTCC 5158, to obtain the corresponding (S)-aryl ethanols with good yield and almost absolute enantioselectivity. The catalytic ability of this microbial strain for acetophenone reduction has been examined and also various parameters of the bioreduction reaction have been optimized. Studies on the catalytic performance showed that this microorganism is capable of carrying out the reduction in a broad range of pH (3-10) and temperature (25-40 degrees C), making it a more versatile biocatalyst. The preparative scale bioreduction of acetophenone using resting cells of Candida tropicalis yielded S-(-)-1-phenyl ethanol with 43% yield and >99% enantiomeric excess.
Soni
Pankaj
P
Biocatalysis laboratory, Department of Pharmaceutical Technology, National Institute of Pharmaceutical Education and Research, Nagar, India.
Banerjee
Uttam Chand
UC
D000098:Acetophenones / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D002175:Candida / Q000145:classification* / Q000378:metabolism*; D013045:Species Specificity; D013237:Stereoisomerism
D000098:Acetophenones; D000429:Alcohol Oxidoreductases; C038699:acetophenone