title: Involvement of protein kinase C in the PACAP-induced differentiation of neural stem cells into astrocytes.
abstract: Expression of members of the conventional protein kinase C (cPKC) family in the differentiation of mouse neural stem cells (NSCs) induced by pituitary adenylate cyclase-activating polypeptide (PACAP) was investigated. In particular, expression of the alpha and beta subtypes of cPKC in NSCs was observed. In response to activation by PACAP, cPKCbeta transiently increased twofold by day 2 and returned to basal levels by day 4, suggesting that cPKCbeta might be responsible for the differentiation process.
authors.affiliation: Laboratory of Biochemistry, Yokohama College of Pharmacy, 601 Matanocho, Totsuka-Ku, Yokohama, Kanagawa 245-0066, Japan. s.nakajou@hamayaku.ac.jp.
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.forename: Fusako
authors.initials: F
authors.lastname: Ohno
authors.forename: Seiji
authors.initials: S
authors.lastname: Shioda
authors.forename: Sakae
authors.initials: S
authors.lastname: Kikuyama
authors.forename: Kazuyasu
authors.initials: K
authors.lastname: Nakaya
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nakajo
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000494:pharmacology*; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011493:Protein Kinase C