title: Cloning and expression of two crystal protein genes, cry30Ba1 and cry44Aa1, obtained from a highly mosquitocidal strain, Bacillus thuringiensis subsp. entomocidus INA288.
abstract: Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC50] = 6 ng/ml) and Aedes aegypti (LC50 = 12 ng/ml); however, Cry30Ba crystals were not toxic.
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. ito-t@abs.agr.hokudai.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ito
authors.forename: Tomonori
authors.initials: T
authors.lastname: Ikeya
authors.forename: Ken
authors.initials: K
authors.lastname: Sahara
authors.forename: Hisanori
authors.initials: H
authors.lastname: Bando
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Asano
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects*; D004731:Endotoxins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis