title: Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination.
abstract: DNA double-strand breaks (DSB) can arise during DNA replication, or after exposure to DNA-damaging agents, and their correct repair is fundamental for cell survival and genomic stability. Here, we show that the Smc5-Smc6 complex is recruited to DSBs de novo to support their repair by homologous recombination between sister chromatids. In addition, we demonstrate that Smc5-Smc6 is necessary to suppress gross chromosomal rearrangements. Our findings show that the Smc5-Smc6 complex is essential for genome stability as it promotes repair of DSBs by error-free sister-chromatid recombination (SCR), thereby suppressing inappropriate non-sister recombination events.
authors.affiliation: Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK.
authors.forename: Giacomo
authors.initials: G
authors.lastname: De Piccoli
authors.forename: Felipe
authors.initials: F
authors.lastname: Cortes-Ledesma
authors.forename: Gregory
authors.initials: G
authors.lastname: Ira
authors.forename: Jordi
authors.initials: J
authors.lastname: Torres-Rosell
authors.forename: Stefan
authors.initials: S
authors.lastname: Uhle
authors.forename: Sarah
authors.initials: S
authors.lastname: Farmer
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Hwang
authors.forename: Felix
authors.initials: F
authors.lastname: Machin
authors.forename: Audrey
authors.initials: A
authors.lastname: Ceschia
authors.forename: Alexandra
authors.initials: A
authors.lastname: McAleenan
authors.forename: Violeta
authors.initials: V
authors.lastname: Cordon-Preciado
authors.forename: Andrés
authors.initials: A
authors.lastname: Clemente-Blanco
authors.forename: Felip
authors.initials: F
authors.lastname: Vilella-Mitjana
authors.forename: Pranav
authors.initials: P
authors.lastname: Ullal
authors.forename: Adam
authors.initials: A
authors.lastname: Jarmuz
authors.forename: Beatriz
authors.initials: B
authors.lastname: Leitao
authors.forename: Debra
authors.initials: D
authors.lastname: Bressan
authors.forename: Farokh
authors.initials: F
authors.lastname: Dotiwala
authors.forename: Alma
authors.initials: A
authors.lastname: Papusha
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhao
authors.forename: Kyungjae
authors.initials: K
authors.lastname: Myung
authors.forename: James E
authors.initials: JE
authors.lastname: Haber
authors.forename: Andrés
authors.initials: A
authors.lastname: Aguilera
authors.forename: Luis
authors.initials: L
authors.lastname: Aragón
mesh_terms: D018797:Cell Cycle Proteins / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D042822:Genomic Instability; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D012854:Sister Chromatid Exchange
chemical_list: D018797:Cell Cycle Proteins; C486627:SMC5 protein, S cerevisiae; C486631:SMC6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific