Elucidation of the beta-carotene hydroxylation pathway in Arabidopsis thaliana.
The first dedicated step in plant xanthophyll biosynthesis is carotenoid hydroxylation. In Arabidopsis thaliana, this reaction is performed by both heme (LUT1 and LUT5) and non-heme (CHY1 and CHY2) hydroxylases. No mutant completely abolishing alpha- or beta-carotene hydroxylation has been described to date. We constructed double and triple mutant combinations in CHY1, CHY2, LUT1, LUT5 and LUT2 (lycopene epsilon-cyclase). In chy1chy2lut2, 80% of leaf carotenoids is represented by beta-carotene. In chy1chy2lut5, beta-carotene hydroxylation is completely abolished, while hydroxylation of the beta-ring of alpha-carotene is still observed. The data are consistent with a role of LUT5 in beta-ring hydroxylation, and with the existence of an additional hydroxylase, acting on the beta-ring of alpha-, but not beta-carotene.
Fiore
Alessia
A
Ente per le Nuove tecnologie, l'Energia e l'Ambiente (ENEA), Dipartimento Biotecnologie, Centro Ricerche Casaccia, C.P. 2400, Roma 00100, Italy.
Dall'osto
Luca
L
Fraser
Paul D
PD
Bassi
Roberto
R
Giuliano
Giovanni
G
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D017343:Genes, Plant; D006900:Hydroxylation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019207:beta Carotene / Q000378:metabolism*
D017931:DNA Primers; D019207:beta Carotene