title: Formation of glucoside conjugate of acetaminophen by fungi separated from soil.
abstract: The phase II metabolite of acetaminophen in filamentous fungi and actinomycetes separated from soil was investigated. Fifty-four filamentous fungi and twenty-seven actinomycetes were screened to transform acetaminophen. The metabolites of acetaminophen were assayed using liquid chromatography-tandem mass spectrometry. The only metabolite was subject to enzymatic hydrolysis to confirm its structure. Acetaminophen was converted into glucoside conjugate, by filamentous fungi JX1-60, LN17-2, LN20-1 and the yield of the conjugate was 60.01%, 44.27%, 100%, respectively, and no phase I metabolites were detected. Glucoside conjugation of acetaminophen in filamentous fungi differs from the phase II metabolism of glucuronidation in humans. The fungus LN20-1 could be a suitable model to synthesize glucoside conjugate of acetaminophen.
authors.affiliation: Shanghai Drug-Metab Biotech Co., Ltd, People's Republic of China.
authors.forename: H H
authors.initials: HH
authors.lastname: Huang
authors.forename: L H
authors.initials: LH
authors.lastname: Lin
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: X L
authors.initials: XL
authors.lastname: Qi
authors.forename: D F
authors.initials: DF
authors.lastname: Zhong
mesh_terms: D000082:Acetaminophen / Q000737:chemistry / Q000378:metabolism*; D039903:Actinobacteria / Q000378:metabolism; D018712:Analgesics, Non-Narcotic; D002681:China; D002853:Chromatography, Liquid; D005658:Fungi / Q000378:metabolism*; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D050217:Metabolic Detoxication, Phase II; D015394:Molecular Structure; D012988:Soil Microbiology; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D018712:Analgesics, Non-Narcotic; D005960:Glucosides; D000082:Acetaminophen