Optimization of extracellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis.
A Scopulariopsis brevicaulis poultry farm isolate was chosen to study factors influencing keratinase production. The parameters were optimized by factorial design. The highest enzyme production by this fungus was obtained at pH 7.5, a temperature of 30 degrees C and a growth period of 5 weeks. The production of the enzyme was enhanced when the culture medium was supplemented with glucose (1%), sodium nitrate (2%), feather (1.5%) and CaCl(2) (1 mM). According to the responses from the experimental design, the effects of each variable were calculated, and the interactions between them were determined. The experimental values were found to be in accordance with the predicted values, the correlation coefficient is 0.9978.
Anbu
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P
Centre for Advanced studies in Botany, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India.
Gopinath
S C B
SC
Hilda
A
A
Lakshmipriya
T
T
Annadurai
G
G
D000818:Animals; D002384:Catalysis; D003470:Culture Media; D005658:Fungi / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D011200:Poultry / Q000382:microbiology*; D013696:Temperature
D003470:Culture Media; D010447:Peptide Hydrolases; C035855:keratinase