Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes.
A matrix assisted laser desorption/ionization time-of-flight mass spectrometry platform for quantitatively monitoring enzyme activity and screening enzyme inhibitors has been demonstrated. The described method employs a new matrix of oxidized carbon nanotubes. Compared with the traditional fluorescence approach, this label-free method has the advantage of directly identifying the substrates and products in enzymatic reactions. Moreover, the method could be conveniently carried out with any commercial mass spectrometer without modification. We quantitatively monitored the acetylcholinesterase activity and screened acetylcholinesterase inhibitors with a detection rate of about 3.3 s per sample.
Hu
Ligang
L
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, 100085, Beijing, China.|Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Jiang
Guibin
G
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, 100085, Beijing, China. gbjiang@rcees.ac.cn.
Xu
Songyun
S
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Pan
Chensong
C
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Zou
Hanfa
H
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
D000110:Acetylcholinesterase / Q000032:analysis* / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000378:metabolism; D006868:Hydrolysis; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D002800:Cholinesterase Inhibitors; D037742:Nanotubes, Carbon; D000110:Acetylcholinesterase