Molecular mechanisms of cellular mechanics.
Mechanical forces play an essential role in cellular processes as input, output, and signals. Various protein complexes in the cell are designed to handle, transform and use such forces. For instance, proteins of muscle and the extracellular matrix can withstand considerable stretching forces, hearing-related and mechanosensory proteins can transform weak mechanical stimuli into electrical signals, and regulatory proteins are suited to forcing DNA into loops to control gene expression. Here we review the structure-function relationship of four protein complexes with well defined and representative mechanical functions. The first example is titin, a protein that confers passive elasticity on muscle. The second system is the elastic extracellular matrix protein, fibronectin, and its cellular receptor integrin. The third protein system is the transduction apparatus in hearing and other mechanical senses, likely containing cadherin and ankyrin repeats. The last system is the lac repressor protein, which regulates gene expression by looping DNA. This review focuses on atomic level descriptions of the physical mechanisms underlying the various mechanical functions of the stated proteins.
Gao
Mu
M
Beckman Institute, Department of Physics, Center for Biophysics and Computational Biology, College of Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Sotomayor
Marcos
M
Villa
Elizabeth
E
Lee
Eric H
EH
Schulten
Klaus
K
D001696:Biomechanical Phenomena; D002468:Cell Physiological Phenomena; D004247:DNA / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry / Q000502:physiology*; D013329:Structure-Activity Relationship
D011506:Proteins; D004247:DNA