Sensitivity of Aedes albopictus C6/36 cells line for the detection and infectivity titration of dengue virus.
Plaque assay procedure was carried out to determine the infectivity titration of dengue virus using Aedes albopictus C6/36 mosquito cell line. Cells were seeded in 6 wells plates and incubated until monolayer C6/36 cell lines were formed. Each well was exposed to a different dilution of dengue infected culture fluid, followed by addition of first and second overlay medium. Cells were observed under an inverted microscope and plaque staining was carried out. The results showed that countable plaques were not achieved and plaques formed were restricted to the central section due to the significant partial lysis of the C6/36 cells in the plates. However, the plaque assay technique using C6/36 cell line reported here appears to be promising and merits further detailed studies.
Zamree
I
I
Medical Entomology Unit, Infectious Disease Research Centre, Institute for Medical Research, Kuala Lumpur.
Drakes
N
N
Rohani
A
A
Lee
H L
HL
D000330:Aedes / Q000821:virology*; D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D003716:Dengue Virus / Q000254:growth & development* / Q000472:pathogenicity; D010948:Viral Plaque Assay / Q000379:methods*; D014773:Virology / Q000379:methods