ORF18-disrupted mutant of Comamonas testosteroni TA441 accumulates significant amounts of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid and its derivatives after incubation with steroids.
In a steroid degradation gene cluster of Comamonas testosteroni TA441 consisting of ORF18, 17 and tesIHA2A1DEFG, ORF18 was implicated in encoding a CoA-transferase by database searches, but the matching substrate was not clear. In this study, ORF18 was shown to be necessary for conversion of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, a product of hydrolysis of 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-dien-4-oic acid in steroid degradation by TA441. The ORF18-disrupted mutant accumulates 7-hydroxy-9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid and 7,12-dihydroxy-9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid when incubated with chenodeoxycholic acid and cholic acid, respectively.
Horinouchi
Masae
M
RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. masae@postman.riken.go.jp
Hayashi
Toshiaki
T
Koshino
Hiroyuki
H
Kudo
Toshiaki
T
D002851:Chromatography, High Pressure Liquid; D020604:Comamonas testosteroni / Q000235:genetics / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D009635:Norandrostanes; D016366:Open Reading Frames; D013739:Testosterone / Q000378:metabolism*
C514444:9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid; D009635:Norandrostanes; D013739:Testosterone