Hair follicle reformation induced by dermal papilla cells from human scalp skin.
To investigate the possibility of hair follicle reformation induced by dermal papilla cells in vivo and in vitro. Dermal papilla cells, dermal sheath cells obtained from human scalp skin by enzyme digestion were mixed with collagen to form mesenchymal cell-populated collagen gels. Superior and inferior epithelial cells and bulb matrical cells were then cultured on these gels by organotypic culture to recombine bilayer artificial skins. Dermal papilla cells and outer root sheath keratinocytes were mingled together and transplanted under subcutaneous tissue of the dorsal skin of nude mice. The results of histologic examination was observed with HE stain. These recombinants by organotypic culture all reformed bilayer structure like nature skin. Hair follicle-like structure reformation was found in dermal sheath cell-populated collagen gel when combined with superior or inferior epithelial cells. Dermal papilla cells also induced superior and inferior epithelial cells to form hair follicle on nude mice. Low passage dermal papilla cells mixed with hair follicle epithelial cells reformed many typical hair follicle structures and produced hair fibres after transplantation on nude mice. The dermal part of hair follicle, such as dermal papilla cells and dermal sheath cells, has the ability to induce hair follicle formation by interaction with the epithelial cells of hair follicle.
Wu
Jin-Jin
JJ
Department of Dermatology, Institute of Battle Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, People's Republic of China. wjjjj@163.com
Zhu
Tang-You
TY
Lu
Yuan-Gang
YG
Liu
Rong-Qing
RQ
Mai
Yue
Y
Cheng
Bo
B
Lu
Zhong-Fa
ZF
Zhong
Bai-Yu
BY
Tang
Shu-Qian
SQ
D000818:Animals; D002450:Cell Communication; D018929:Cell Culture Techniques; D017690:Cell Transplantation / Q000379:methods / Q000502:physiology*; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology*; D018859:Hair Follicle / Q000033:anatomy & histology / Q000254:growth & development*; D006651:Histocytochemistry; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D012535:Scalp / Q000166:cytology* / Q000502:physiology; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods