title: Proteomic methods in nutrition.
abstract: OBJECTIVE
  Proteomics, the comprehensive analysis of a protein complement in a cell, tissue or biological fluid at a given time, is a key player in the family of -omic disciplines, which encompass genomics (gene analysis), transcriptomics (gene expression analysis) and metabolomics (metabolite profiling). This review summarizes the state of the art of proteomics technology and puts it into perspective for food-related research. Learning from proteomic experiences in the pharmaceutical context, this article may help to translate proteomics into nutrition and health.
  
  
  RESULTS
  Mass spectrometric technology has progressed enormously with regard to mass accuracy, resolution and peptide sequencing power. Likewise, upstream separation, depletion and enrichment techniques now allow us to deal with the large complexity and wide dynamic range of proteomic samples more efficiently. Consequently, proteomic studies now provide a broader, but still far from complete, coverage of a given proteome.
  
  
  CONCLUSIONS
  Proteomics adapted and applied to the context of nutrition and health has the potential to deliver biomarkers for health and comfort, reveal early indicators of disease disposition, assist in differentiating dietary responders from non-responders, and, last but not least, discover bioactive, beneficial food components.
authors.affiliation: Bioanalytical Science Department, Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland. martin.kussmann@rdls.nestle.com
authors.forename: Martin
authors.initials: M
authors.lastname: Kussmann
authors.forename: Michael
authors.initials: M
authors.lastname: Affolter
mesh_terms: D015415:Biomarkers; D005524:Food Technology; D058870:Food, Organic; D006262:Health; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009747:Nutritional Physiological Phenomena; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers