A binary DNA probe for highly specific nucleic Acid recognition.
A new concept for nucleic acid probe design is reported. The extremely high selectivity of the probe is predetermined by cooperative hybridization of the two relatively short (10 nucleotide) DNA hairpin fragments to the analyte. A binary DNA probe fluorescently reports the presence of 0.5% of the analyte in excess amount of a single base substituted oligodeoxyribonucleotide and distinguishes single nucleotide substitutions at any position of a 20-mer oligonucleotide at room temperature.
Kolpashchikov
Dmitry M
DM
Division of Experimental Therapeutics, Department of Medicine, Columbia University, Box 84, 630W 168th Street, New York, New York 10032, USA. dk2111@clumbia.edu
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000032:analysis*; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D004247:DNA