title: Acute ethanol administration decreases GAP-43 and phosphorylated-GAP-43 in the rat hippocampus.
abstract: Acute alcohol ingestion is well known to have deleterious effects on memory and also known to inhibit long-term potentiation, a putative cellular substrate of memory. In this study, we for the first time revealed that growth-associated protein 43 (GAP-43), which is well known as a presynaptic substrate of protein kinase C and one of the major synaptic plasticity-related genes, was down regulated by single ethanol administration (2.5 g/kg, 15% in saline, i.p.) in the rat hippocampus. Using real-time PCR, we confirmed that GAP-43 mRNA level is significantly decreased 2 h after ethanol administration. GAP-43 and p-GAP-43 (Ser41) immunoreactivities in the hippocampus were also reduced 4 h after ethanol administration. Immunohistochemical study showed that the reduction of GAP-43 and p-GAP-43 expression was associated with the perforant and mossy fibers pathways. These results suggest that the reduction of GAP-43 in the hippocampus might be, at least in part, a cause of memory impairment after acute ethanol ingestion.
authors.affiliation: Department of Anatomy and Neurobiology, Institute of Health Sciences, College of Medicine, Gyeongsang National University, 92 Chilam-dong, Jinju, Kyungnam 660-751, Korea.
authors.forename: Hyun Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Choi
authors.forename: Bo Mi
authors.initials: BM
authors.lastname: Ku
authors.forename: Jihye
authors.initials: J
authors.lastname: Mun
authors.forename: Yeon
authors.initials: Y
authors.lastname: Joo
authors.forename: Jae Yoon
authors.initials: JY
authors.lastname: Han
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Gu Seob
authors.initials: GS
authors.lastname: Roh
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Kang
authors.forename: Gyeong Jae
authors.initials: GJ
authors.lastname: Cho
authors.forename: Wan Sung
authors.initials: WS
authors.lastname: Choi
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002492:Central Nervous System Depressants / Q000008:administration & dosage* / Q000097:blood; D000431:Ethanol / Q000008:administration & dosage* / Q000097:blood; D019922:GAP-43 Protein / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D002492:Central Nervous System Depressants; D019922:GAP-43 Protein; D012333:RNA, Messenger; D000431:Ethanol; D011493:Protein Kinase C