title: A plant peptide encoded by CLV3 identified by in situ MALDI-TOF MS analysis.
abstract: The Arabidopsis CLAVATA3 (CLV3) gene encodes a stem cell-specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applied to in situ Arabidopsis tissues determined the structure of a modified 12-amino acid peptide (MCLV3), which was derived from a conserved motif in the CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying the typical phenotype of transgenic plants overexpressing CLV3. These results suggest that the functional peptide of CLV3 is MCLV3.
authors.affiliation: Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kondo
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Sawa
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kinoshita
authors.forename: Satoko
authors.initials: S
authors.lastname: Mizuno
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kakimoto
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
authors.forename: Youji
authors.initials: Y
authors.lastname: Sakagami
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D006909:Hydroxyproline / Q000737:chemistry; D018519:Meristem / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013234:Stem Cells / Q000166:cytology
chemical_list: C118167:AT2G27250 protein, Arabidopsis; D029681:Arabidopsis Proteins; D009842:Oligopeptides; D006909:Hydroxyproline