title: [Detection of rpoB mutations in rifampicin-resistant Mycobacterium kansasii].
abstract: OBJECTIVE
  To detect rifampicin-resistant mutations in Mycobacterium kansasii (M. kansasii).
  
  
  METHODS
  We examined the M. kansasii isolates from sputum of patients at National Hospital Organization Kinki-chuo Chest Medical Center from January 1, 2001 to November 30, 2005 using drug-susceptibility testing, and analyzed 69-bp fragment of rpoB gene in rifampicin-resistant strains.
  
  
  RESULTS
  Three strains from 314 isolates were determined as rifampicin resistant using drug-susceptibility testing. Those strains showed a rise in minimum inhibitory concentration (MIC), and had the mutations in rpoB gene. These point mutations in codons 513 and 516 were common mutations found in rifampicin-resistant clinical isolates of M. tuberculosis.
  
  
  CONCLUSIONS
  We verified the association between rpoB gene mutations and rifampicin resistance in M. kansasii.
authors.affiliation: Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, 1180 Nagasone-cho, Kita-ku, Sakai-shi, Osaka 591-8555 Japan. dustin@kch.hosp.go.jp
authors.forename: Shiomi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Kazunari
authors.initials: K
authors.lastname: Tsuyuguchi
authors.forename: Tomotada
authors.initials: T
authors.lastname: Iwamoto
authors.forename: Motohisa
authors.initials: M
authors.lastname: Tomita
authors.forename: Masaji
authors.initials: M
authors.lastname: Okada
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Sakatani
mesh_terms: D024881:Drug Resistance, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D009154:Mutation; D019909:Mycobacterium kansasii / Q000187:drug effects / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology*
chemical_list: D012293:Rifampin