title: Excision of the Sinorhizobium meliloti group II intron RmInt1 as circles in vivo.
abstract: Excision of group II introns as circles has been described only for a few eukaryotic introns and little is known about the mechanisms involved, the relevance or consequences of the process. We report that splicing of the bacterial group II intron RmInt1 in vivo leads to the formation of both intron lariat and intron RNA circles. We determined that besides being required for the intron splicing reaction, the maturase domain of the intron-encoded protein also controls the balance between lariat and RNA intron circle production. Furthermore, comparison with in vitro self-splicing products indicates that in vivo, the intron-encoded protein appears to promote the use of a correct EBS1/IBS1 intron-exon interaction as well as cleavage at, or next to, the expected 3' splice site. These findings provide new insights on the mechanism of excision of group II introns as circles.
authors.affiliation: Grupo de Ecología Genética, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Calle Professor Albareda 1, 18008 Granada, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Molina-Sánchez
authors.forename: Francisco
authors.initials: F
authors.lastname: Martinez-Abarca
authors.forename: Nicolás
authors.initials: N
authors.lastname: Toro
mesh_terms: D017398:Alternative Splicing; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D005091:Exons; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D017434:Protein Structure, Tertiary; D012313:RNA / Q000235:genetics*; D012326:RNA Splicing; D016962:Sinorhizobium meliloti / Q000235:genetics*
chemical_list: D017931:DNA Primers; D012313:RNA