Spectrophotometric method for exploring 3-methyl-2-butene-1-thiol (MBT) formation in lager.
The disappearance of riboflavin absorbance at 445 nm from beers or model beers on light exposure is directly linked to light-struck character formation. The addition of (+)-catechin, (-)-epicatechin, tryptophol, or ascorbic acid was able to reduce, but not stop, absorbance loss or light-struck character formation in either model beer or mainstream lager that was exposed to light. When isohumulone was present in model beer, the inhibitory effect of (+)-catechin, (-)-epicatechin, or tryptophol decreased with increasing isohumulone. The spectrophotometric method used in this study is a simple and effective method for determining light-struck susceptibility.
Pozdrik
Richard
R
School of Civil and Chemical Engineering, RMIT University, P.O. Box 2476V, Melbourne 3001, Australia.
Roddick
Felicity A
FA
Rogers
Peter J
PJ
Nguyen
Thang
T
D001205:Ascorbic Acid / Q000494:pharmacology; D001515:Beer / Q000032:analysis*; D002073:Butanes / Q000737:chemistry*; D002392:Catechin / Q000494:pharmacology; D003517:Cyclopentanes / Q000032:analysis; D007211:Indoles / Q000494:pharmacology; D008027:Light; D010777:Photochemistry; D012256:Riboflavin / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D000438:Alcohols
D001205:Ascorbic Acid; D002073:Butanes; D002392:Catechin; D003517:Cyclopentanes; D007211:Indoles; D012256:Riboflavin; D013438:Sulfhydryl Compounds; C005949:tryptophol; D000438:Alcohols; C081209:isohumulone; C512562:3-methyl-2-butene-1-thiol