Pluronic L81 enhances triacylglycerol accumulation in the cytosol and inhibits chylomicron secretion.
Pluronic L81 (PL81) inhibits fat absorption, and other Pluronic copolymers help overcome drug resistance in cancer cells. To understand how PL81 acts, we synthesized a radiolabeled analog, [14C]PL81, and showed that it was structurally similar to PL81 based on (1)H NMR as well as mass spectrometric analysis. [14C]PL81 inhibited the secretion of chylomicrons (CMs), lipoproteins essential for fat absorption, by differentiated Caco-2 cells similar to PL81. Moreover, PL81 competed with the cellular uptake of [14C]PL81. Thus, [14C]PL81 and PL81 behave similarly in these physiologic assays. Uptake of [14C]PL81 by Caco-2 cells was concentration-, time-, and temperature-dependent and occurred mainly from the apical side. Intracellularly, it was assimilated in the cytosol. Cells excreted PL81 toward the apical side via a pathway partially sensitive to verapamil. Small amounts were secreted toward the basolateral side unassociated with CM, and this secretion was unaffected by the inhibition of CM assembly. Nonetheless, PL81 significantly inhibited the secretion of triacylglycerols (TGs) and phospholipids as part of CM. PL81-treated cells showed decreased activity of microsomal triglyceride transfer protein and accumulated more TGs, but not phospholipids, in their cytosol. We propose that Pluronic copolymers act by interfering with the export of molecules from the cytosol. They inhibit fat absorption by decreasing TG transport to the endoplasmic reticulum and increase drug efficacy against cancer cells by competing for their excretion.
Fatma
Sumbul
S
Department of Anatomy & Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA.
Yakubov
Reuven
R
Anwar
Kamran
K
Hussain
M Mahmood
MM
D001692:Biological Transport; D018938:Caco-2 Cells; D002499:Centrifugation, Density Gradient; D002914:Chylomicrons / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006801:Humans; D008956:Models, Chemical; D020442:Poloxamer / Q000494:pharmacology*; D013347:Subcellular Fractions; D013501:Surface-Active Agents / Q000494:pharmacology*; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism*
D002914:Chylomicrons; D013501:Surface-Active Agents; D014280:Triglycerides; D020442:Poloxamer