Quantitative evaluation of bone marrow angiogenesis in idiopathic myelofibrosis.
Bone marrow angiogenesis in patients with idiopathic myelofibrosis (IM) was evaluated by using a quantitative morphometric analysis test system. IM specimens had a significantly higher degree of bone marrow microvessel density (MVD) compared with control specimens. The higher degree of angiogenesis was associated with increased expression of basic fibroblast growth factor in megakaryocytes and endothelial cells in the IM bone marrow specimens. Increased vascular endothelial growth factor expression was seen in some cases of the prefibrotic form of IM. Morphometric quantitative measurements revealed that the MVD was reduced progressively in 4 cases of IM after allogeneic stem cell transplantation. These findings suggest that angiogenesis likely has an important role in the pathogenesis of IM.
Ni
Hongyu
H
Department of Pathology, Section of Hematology/Oncology, University of Illinois Cancer Center, University of Illinois College of Medicine, Chicago, IL, USA.
Barosi
Giovanni
G
Hoffman
Ronald
R
D000328:Adult; D000368:Aged; D015415:Biomarkers; D001853:Bone Marrow / Q000098:blood supply / Q000473:pathology*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007124:Immunoenzyme Techniques; D008533:Megakaryocytes / Q000000981:diagnostic imaging / Q000378:metabolism; D008833:Microcirculation; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000473:pathology*; D055728:Primary Myelofibrosis / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D011859:Radiography; D033581:Stem Cell Transplantation; D014184:Transplantation, Homologous; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D015415:Biomarkers; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2