title: A newly developed bioartificial pancreas successfully controls blood glucose in totally pancreatectomized diabetic pigs.
abstract: Construction of a safe and functional bioartificial pancreas (BAP) that provides an adequate environment for islet cells may be an important approach to treating diabetic patients. Various types of BAP devices have been developed, but most of them involve extravascular implantation of islets in microcapsules or diffusion chambers. These devices have poor diffusive exchange between the islets and blood, and often rupture. To overcome these problems, we developed a new type of BAP composed of polyethylene-vinyl alcohol (EVAL) hollow fibers that are permeable to glucose and insulin and a poly-amino-urethane-coated, non-woven polytetrafluoroethylene (PTFE) fabric that allows cell adhesion. Porcine islets attached to the surface of the PTFE fabric, but not to the surface of the EVAL hollow fibers, allowing nutrient and oxygen exchange between blood flowing inside the fibers and cells outside. We inoculated this BAP with porcine islets and connected it to the circulation of totally pancreatectomized diabetic pigs. We found that blood glucose levels were reduced to a normal range and general health was improved, resulting in longer survival times. In addition, regulation of insulin secretion from the BAP was properly controlled in response to glucose both in vitro and in vivo. These results indicate that our newly developed BAP may be a potential therapy for the treatment of diabetes in humans.
authors.affiliation: Department of Surgery, Graduate School of Medicine and Dentistry, Okayama University, Okayama, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ikeda
authors.forename: Naoya
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Shuhei
authors.initials: S
authors.lastname: Nakaji
authors.forename: Chen
authors.initials: C
authors.lastname: Yong
authors.forename: Teru
authors.initials: T
authors.lastname: Okitsu
authors.forename: Mizuko
authors.initials: M
authors.lastname: Oshita
authors.forename: Shinichi
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Noguchi
authors.forename: Michiki
authors.initials: M
authors.lastname: Narushima
authors.forename: Kimiaki
authors.initials: K
authors.lastname: Tanaka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miki
authors.forename: Jorge David
authors.initials: JD
authors.lastname: Rivas-Carrillo
authors.forename: Alejandro
authors.initials: A
authors.lastname: Soto-Gutierrez
authors.forename: Nalu
authors.initials: N
authors.lastname: Navarro-Alvarez
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanaka
authors.forename: Hee-Sook
authors.initials: HS
authors.lastname: Jun
authors.forename: Noriaki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Yoon
mesh_terms: D000818:Animals; D021522:Bioartificial Organs; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000628:therapy*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D008567:Membranes, Artificial; D051379:Mice; D019397:Pancreas, Artificial; D013552:Swine
chemical_list: D001786:Blood Glucose; D007328:Insulin; D008567:Membranes, Artificial