title: Proteomic analysis of pulmonary sclerosing hemangioma.
abstract: Sclerosing hemangioma (SH) is a rare benign pulmonary tumor derived from the primitive respiratory epithelium. However, the pathogenesis of SH has not yet been clear. Surfactant protein, thyroid transcription factor-1, epithelial membrane antigen, cytokeratin, and vimentin have been identified in SH by immunohistochemistry and electron microscopy. To identify proteins specifically regulated in SH, 2-D PAGE was performed using SH and paired normal tissues. Ten selected differentially expressed protein spots were identified by PMF, MALDI-TOF-MS, and database searching. Apolipoprotein A-1, antizyme inhibitor, heat shock 27-kDa protein 1, and antioxidant proteins, such as peroxiredoxin II (Prx II) and GST, were identified among the down-regulated proteins in SH. Western blot and immunohistochemistry confirmed reduced expressions of Prx II and GST in SH versus normal lung tissue. This study is the first report on the reduced expressions of Prx II and GST in SH.
authors.affiliation: Korea Lung Tissue Bank, Korea University, Seoul, Korea.
authors.forename: Lian-Jin
authors.initials: LJ
authors.lastname: Jin
authors.forename: Bong Kyung
authors.initials: BK
authors.lastname: Shin
authors.forename: Woon Yong
authors.initials: WY
authors.lastname: Jung
authors.forename: Hyun-Juu
authors.initials: HJ
authors.lastname: Lee
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Cho
authors.forename: Joung-Ho
authors.initials: JH
authors.lastname: Han
authors.forename: Seong-Yeon
authors.initials: SY
authors.lastname: Ha
authors.forename: Ae-Ree
authors.initials: AR
authors.lastname: Kim
authors.forename: Young
authors.initials: Y
authors.lastname: Sik Kim
authors.forename: In
authors.initials: I
authors.lastname: Sun Kim
authors.forename: Chang-Sub
authors.initials: CS
authors.lastname: Uhm
authors.forename: Han Kyeom
authors.initials: HK
authors.lastname: Kim
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000737:chemistry / Q000378:metabolism; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism; D054464:Peroxiredoxins; D040901:Proteomics; D047868:Pulmonary Sclerosing Hemangioma / Q000378:metabolism* / Q000473:pathology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D016328:NF-kappa B; D013438:Sulfhydryl Compounds; D010544:Peroxidases; D054464:Peroxiredoxins