title: Two GacA-dependent small RNAs modulate the quorum-sensing response in Pseudomonas aeruginosa.
abstract: In Pseudomonas aeruginosa, the GacS/GacA two-component system positively controls the quorum-sensing machinery and the expression of extracellular products via two small regulatory RNAs, RsmY and RsmZ. An rsmY rsmZ double mutant and a gacA mutant were similarly impaired in the synthesis of the quorum-sensing signal N-butanoyl-homoserine lactone, the disulfide bond-forming enzyme DsbA, and the exoproducts hydrogen cyanide, pyocyanin, elastase, chitinase (ChiC), and chitin-binding protein (CbpD). Both mutants showed increased swarming ability, azurin release, and early biofilm development.
authors.affiliation: Département de Microbiologie Fondamentale, Université de Lausanne, Building Biophore, CH-1015 Lausanne, Switzerland.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kay
authors.forename: Bérénice
authors.initials: B
authors.lastname: Humair
authors.forename: Valérie
authors.initials: V
authors.lastname: Dénervaud
authors.forename: Kathrin
authors.initials: K
authors.lastname: Riedel
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Spahr
authors.forename: Leo
authors.initials: L
authors.lastname: Eberl
authors.forename: Claudio
authors.initials: C
authors.lastname: Valverde
authors.forename: Dieter
authors.initials: D
authors.lastname: Haas
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C091100:GacA protein, Bacteria; D012329:RNA, Bacterial; D012269:Ribosomal Proteins