[The estimation of Pneumocystis carinii in HIV-infected patients occurrence based on three different methods].
The frequency of Pneumocystis carinii occurrence in BAL of 38 HIV-infected patients was determined with three different method. BAL sediments were stained with Giemsa method, silvered according to Gomori-Grocott method and studied with indirect immunofluorescence assay. Using Giemsa method staining Pneumocystis carinii was diagnosed in 81.6% of patients, in Gomori-Grocott method--in 31.6% of patients, but results of indirect immunofluorescence assay were positive only in 23.,7%. In our study staining BAL sediments with Giemsa method allowed to detect Pneumocystis carinii in the highest percentage of examined patients.
Ciarkowska
J
J
Katedra i Zakład Ogólnej Biologii Lekarskiej Slaskiej Akademii Medycznej, 41-808 Zabrze, ul. Jordana 19.
Spausta
G
G
Wiczkowski
A
A
Adamek
B
B
Beniowski
M
M
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology*; D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D015897:Comorbidity; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D045363:Pneumocystis carinii / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis* / Q000453:epidemiology*; D015995:Prevalence; D012680:Sensitivity and Specificity; D013194:Staining and Labeling