Cloning and expression of two crystal protein genes, cry30Ba1 and cry44Aa1, obtained from a highly mosquitocidal strain, Bacillus thuringiensis subsp. entomocidus INA288.
Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC50] = 6 ng/ml) and Aedes aegypti (LC50 = 12 ng/ml); however, Cry30Ba crystals were not toxic.
Ito
Takeshi
T
Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. ito-t@abs.agr.hokudai.ac.jp
Ikeya
Tomonori
T
Sahara
Ken
K
Bando
Hisanori
H
Asano
Shin-ichiro
S
D000330:Aedes / Q000187:drug effects*; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects*; D004731:Endotoxins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis