[Suggestions concerning serological diagnosis of echinococcosis in Poland].
It has been suggested to change the present way of serological diagnosis of echinococcosis in Poland which utilizes immunoelectrophoresis for confirmation of positive results in a screening test. Diagnostic procedure should be indicated with a sensitive test, such as indirect haemagglutination or immunoenzymatic assay, and every positive result should be verifies later using a Western-blot method in order to detect the most specific reaction with 8 kDa fraction of Echinococcus antigen. Further diferentiation of the infecting species may be accomplished with the aid of Em2 plus ELISA test which detects antibodies specific for E. multilocularis.
Bitkowska
E
E
Zakład Parazytologii Lekarskiej Państwowego Zakładu Higieny 00-791 Warszawa, ul. Chocimska 24.
Gołab
E
E
Płonka
W
W
Dzbeński
T H
TH
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western / Q000379:methods; D004444:Echinococcosis, Hepatic / Q000175:diagnosis*; D004446:Echinococcus / Q000145:classification / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011044:Poland; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000947:Antigens, Helminth; D015415:Biomarkers