Lamotrigine and phenytoin, but not amiodarone, impair peripheral chemoreceptor responses to hypoxia.
Amiodarone, lamotrigine, and phenytoin, common antiarrhythmic and antiepileptic drugs, inhibit a persistent sodium current in neurons (I(NaP)). Previous results from our laboratory suggested that I(NaP) is critical for functionality of peripheral chemoreceptors. In this study, we determined the effects of therapeutic levels of amiodarone, lamotrigine, and phenytoin on peripheral chemoreceptor and ventilatory responses to hypoxia. Action potentials (APs) of single chemoreceptor afferents were recorded using suction electrodes advanced into the petrosal ganglion of an in vitro rat peripheral chemoreceptor complex. AP frequency (at Po(2) approximately 150 Torr and Po(2) approximately 90 Torr), conduction time, duration, and amplitude were measured before and during perfusion with therapeutic dosages of the drug or vehicle. Hypoxia-induced catecholamine secretion within the carotid body was measured using amperometry. With the use of whole body plethysmography, respiration was measured in unanesthesized rats while breathing room air, 12% O(2), and 5% CO(2), before and after intraperitoneal administration of amiodarone, lamotrigine, phenytoin, or vehicle. Lamotrigine (10 microM) and phenytoin (5 microM), but not amiodarone (5 microM), decreased chemoreceptor AP frequency without affecting other AP parameters or magnitude of catecholamine secretion. Similarly, lamotrigine (5 mg/kg) and phenytoin (10 mg/kg) blunted the hypoxic but not the hypercapnic ventilatory response. In contrast, amiodarone (2.5 mg/kg) did not alter the ventilatory response to hypoxia or hypercapnia. We conclude that lamotrigine and phenytoin at therapeutic levels impair peripheral chemoreceptor function and ventilatory response to acute hypoxia. These are consistent with I(NaP) serving an important function in AP generation and may be clinically important in the care of patients using these drugs.
Faustino
Edward Vincent S
EV
Department of Pediatrics, Yale University School of Medicine, 333 Cedar St., PO Box 208064, New Haven, CT 06520-8064, USA. vince.faustino@yale.edu
Donnelly
David F
DF
D000200:Action Potentials / Q000187:drug effects; D000638:Amiodarone / Q000008:administration & dosage*; D000818:Animals; D000927:Anticonvulsants / Q000008:administration & dosage; D002628:Chemoreceptor Cells / Q000187:drug effects / Q000503:physiopathology*; D005260:Female; D006935:Hypercapnia / Q000188:drug therapy / Q000503:physiopathology*; D000860:Hypoxia / Q000188:drug therapy / Q000503:physiopathology*; D000077213:Lamotrigine; D008297:Male; D010672:Phenytoin / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015656:Respiratory Mechanics / Q000187:drug effects*; D014227:Triazines / Q000008:administration & dosage*; D014665:Vasodilator Agents / Q000008:administration & dosage
D000927:Anticonvulsants; D014227:Triazines; D014665:Vasodilator Agents; D010672:Phenytoin; D000638:Amiodarone; D000077213:Lamotrigine