Interactions of ribosomal protein S1 with DsrA and rpoS mRNA.
Ribosomal protein S1 is shown to interact with the non-coding RNA DsrA and with rpoS mRNA. DsrA is a non-coding RNA that is important in controlling expression of the rpoS gene product in Escherichia coli. Photochemical crosslinking, quadrupole-time of flight tandem mass spectrometry, and peptide sequencing have identified an interaction between DsrA and S1 in the 30S ribosomal subunit. Purified S1 binds both DsrA (K(obs) approximately 6 x 10(6) M(-1)) and rpoS mRNA (K(obs) approximately 3 x 10(7) M(-1)). Ribonuclease probing experiments indicate that S1 binding has a weak but detectable effect on the secondary structure of DsrA or rpoS mRNA.
Koleva
Rositsa I
RI
Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, ME 04704, USA.
Austin
Christina A
CA
Kowaleski
Jeffrey M
JM
Neems
Daniel S
DS
Wang
Leyi
L
Vary
Calvin P H
CP
Schlax
Paula Jean
PJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D024202:Electrophoretic Mobility Shift Assay; D029968:Escherichia coli Proteins / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism*; D058727:RNA, Small Untranslated; D022661:RNA, Untranslated / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D012808:Sigma Factor / Q000235:genetics*
D001426:Bacterial Proteins; C405431:DsrA RNA, E coli; D029968:Escherichia coli Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D058727:RNA, Small Untranslated; D022661:RNA, Untranslated; D012269:Ribosomal Proteins; D012808:Sigma Factor; C015494:ribosomal protein S1; C069567:sigma factor KatF protein, Bacteria; D012259:Ribonuclease, Pancreatic