[The experiment of porcine keratinocytes cultured on porcine small intestinal submucosa in vitro].
OBJECTIVE
To investigate the feasibility of acellular porcine small intestinal submucosa (SIS as bioscaffold of tissue engineering skin.


METHODS
The second passage keratinocytes were seeded on SIS, after seeded for 11, 13, 15, 17 days, the keratinocytes/SIS composites were observed by dye directly, histopathology, immunohistochemical studies with monoclonal antibodies against laminin and transmission electron microscope (TEM).


RESULTS
At eleventh day, keratinocytes were growth very well on the surface of SIS, there are 2-3 cell layers on partial of the SIS surface, the continued expression of laminin can be detected between the keratinocytes and the surface of SIS. After 13, 15, 17 days this stratified structure increased, cells contact more closely, the tonofibrils in cells, desmosome between cells and the basal membrane between the keratinocytes and the surface of SIS can be seen with TEM.


CONCLUSIONS
SIS is a kind of good bioscaffold in the culture of porcine keratinocytes in vitro.
Fan
Wei-jie
WJ
Division of Stem Cell and Tissue Engineering State Key Laboratory of Biotherapy, West China Hospital, Sichuan University.
Yang
Zhi-ming
ZM
Li
Xiu-qun
XQ
Wang
Zhen
Z
Zhi
Wei
W
Qiu
Lin
L
D000818:Animals; D001672:Biocompatible Materials; D018929:Cell Culture Techniques; D002470:Cell Survival; D002478:Cells, Cultured; D007413:Intestinal Mucosa / Q000166:cytology*; D007421:Intestine, Small / Q000166:cytology; D015603:Keratinocytes / Q000166:cytology*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials