Permeabilization and lysis induced by bacteriocins and its effect on aldehyde formation by Lactococcus lactis.
Permeabilization induced by lacticin 3147, lactococcins A, B and M, enterocin AS-48 and nisin, bacteriocins described as cell membrane-pore forming and lytic agents, enhanced in all cases aldehyde formation by Lactococcus lactis IFPL730. Nevertheless, the conversion of isoleucine into 2-methylbutyraldehyde depended not only on the degree of permeabilization but also on the bacteriocin that caused the cell membrane damage. The highest values of 2-methylbutyraldehyde corresponded to cell suspensions containing lacticin 3147 and lactococcins, treatments that provoked further lysis in addition to induced permeabilization.
Martínez-Cuesta
M Carmen
MC
Department of Dairy Science and Technology, Instituto del Frío (CSIC), José Antonio Novais 10, Ciudad Universitaria, 28040 Madrid, Spain. ifrcm94@if.csic.es
Requena
Teresa
T
Peláez
Carmen
C
D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism*; D001430:Bacteriocins / Q000145:classification / Q000494:pharmacology*; D001433:Bacteriolysis / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D002611:Cheese / Q000382:microbiology; D013294:Lactococcus lactis / Q000378:metabolism*; D013649:Taste
D000447:Aldehydes; D001430:Bacteriocins; C082508:lacticin 481