[The enzymes of carbohydrates metabolism from Cysttidicola farionis (Cystidicolidae)].
The content of glycogen, glucose and trehalose was measured in larvae and adults of Cystidicola farionis, the parasite isolated from the swim bladder of Osmerus eperlanus from Vistula Lagoon. Activity of glycogen phosphorylase, alpha-amylase, glucoamylase, maltase, trehalase, and trehalose phosphorylase were measured. The highest activity was recorded for alpha-amylase 10.07 +/- 0.97 mu/mg and 7.47 +/- 0.24 mu/mg, next maltase 1.34 +/- 0.63 micromol/mg and 2.06 +/- 1.65 micronol/mg respectively for larvae and adults. The activity of glucoamylase was nearly the same for adults and larvae (about 0.20 micromol/mg). The trehalase activity was higher at adults (0.49 +/- 0.42 micromol/mg) than at larvae (0.18 +/- 0.12 micromol/mg). The activity of glycogen phosphorylase was much higher at larvae (3.58 +/- 1.49 micromol/mg) than at adults parasite (0.10 +/- 0.02 micromol/mg). The trehalose phosphorylase was present in both stages of parasite, but its activity was low. The content of glycogen and glucose was two-times higher in the adults' body than in larvae.
Zółtowska
K
K
Zakład Biochemii, Wydział Biologii, UWM, 10-561 Olsztyn, ul. Zołnierska 14.
Lopieńska
E
E
Rokicki
J
J
Dmitryjuk
M
M
D000818:Animals; D050260:Carbohydrate Metabolism / Q000502:physiology; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D006003:Glycogen / Q000302:isolation & purification; D024981:Glycogen Phosphorylase / Q000378:metabolism*; D007814:Larva / Q000737:chemistry / Q000378:metabolism; D009348:Nematoda / Q000737:chemistry / Q000201:enzymology*; D014198:Trehalase / Q000378:metabolism*; D014199:Trehalose / Q000302:isolation & purification; D000516:alpha-Amylases / Q000378:metabolism*
D006003:Glycogen; D014199:Trehalose; D024981:Glycogen Phosphorylase; D000516:alpha-Amylases; D014198:Trehalase; D005087:Glucan 1,4-alpha-Glucosidase