Dietary ascorbic acid may be necessary for enhancing the immune response in Siberian sturgeon (Acipenser baerii), a species capable of ascorbic acid biosynthesis.
Siberian sturgeon (Acipenser baerii Brandt) is one of few fish species capable of synthesizing ascorbic acid (AA); however, it is unknown whether dietary AA is still necessary for this species to generate an optimal non-specific immune response, as is found in most teleostean species that do not synthesize AA. Sturgeon fed either 0 or 500 mg AA (AA polyphosphate) equivalent kg(-1) diets for 2 weeks were then either sham or experimentally injected with Aeromonas hydrophila lipopolysaccharide (LPS) to mimic infection and therefore instigate a non-specific immune response. No significant differences were found in growth and survival throughout the duration of the experiment between sham and LPS groups and between both diets. Two and eight days after injection, serum lysozyme levels were elevated in both LPS and non-LPS groups. Respiratory burst was not affected by dietary AA and significantly higher in LPS groups over non-LPS groups; however, dietary AA may have enhanced the effects seen in the LPS injected fish fed the AA supplemented diet. There were no significant differences in renal l-gulono-1, 4-lactone oxidase (GLO) activity between dietary and LPS treatments, but fish exposed to LPS without dietary AA demonstrated an inter-organ transfer of AA from posterior kidney to liver. Our results indicate that dietary AA may be conditionally necessary for Siberian sturgeon to achieve optimal immune response, particularly in early developmental stages, information imperative to developing successful aquaculture programs for sturgeon species.
Xie
Zhigang
Z
Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, 100875 Beijing, PR China.
Niu
Cuijuan
C
Zhang
Zuobing
Z
Bao
Lei
L
D000818:Animals; D001205:Ascorbic Acid / Q000096:biosynthesis / Q000494:pharmacology*; D004032:Diet; D005399:Fishes / Q000254:growth & development / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D050544:L-Gulonolactone Oxidase / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism
D008070:Lipopolysaccharides; D050544:L-Gulonolactone Oxidase; D009113:Muramidase; D001205:Ascorbic Acid