title: High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand.
abstract: The rumen flukes of 37 cows (Bos indicus) from Chiang Mai and Lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). Three species were found: Paramphistomum epiclitum, Orthocoelium streptocoelium, and Fischoederius elongatus with prevalences of infection of 75.68%, 48.65%, and 40.54%, respectively. Genomic DNA was amplified by polymerase chain reaction based on the high annealing temperature-random amplification of polymorphic DNA (HAT-RAPD) technique. Five random 10-mer oligonucleotide primers (OPA2, OPA4, OPB18, OPC9, and OPH11) produced distinct banding patterns in three species. No genetic variations in these three species were identified using 10 arbitary primers.
authors.affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50202, Thailand.
authors.forename: Pralongyut
authors.initials: P
authors.lastname: Sripalwit
authors.forename: Chalobol
authors.initials: C
authors.lastname: Wongsawad
authors.forename: Pheravut
authors.initials: P
authors.lastname: Wongsawad
authors.forename: Somboon
authors.initials: S
authors.lastname: Anuntalabhochai
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D017931:DNA Primers / Q000737:chemistry; D018509:DNA, Helminth / Q000032:analysis; D014644:Genetic Variation; D006358:Hot Temperature; D010250:Paramphistomatidae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D015995:Prevalence; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D012417:Rumen / Q000469:parasitology; D013272:Stomach Diseases / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary; D013785:Thailand / Q000453:epidemiology; D014201:Trematode Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth