Toward intrinsically fluorescent proteomimetics: fluorescent probe response to alpha helix structure of poly-gamma-benzyl-L-glutamate.
A fluorescent probe (1), developed for recognition of alpha helical secondary structure, shows a large fluorescence change upon titration with the synthetic protein PBLG. Compared to fluorophores of similar size and shape, 1 displayed the smallest dissociation constant (K(D)=80microM) when titrated with PBLG. These preliminary studies are directed toward developing small molecule proteomimetics that have intrinsic fluorescence and are specific for helical-protein binding-sites.
Hutt
Kenji
K
New Mexico Institute of Mining and Technology, Chemistry, 801 Leroy Avenue, Socorro, NM 87801, USA.
Hernandez
Randy
R
Heagy
Michael D
MD
D005456:Fluorescent Dyes / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D018716:Molecular Mimicry; D015394:Molecular Structure; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D017433:Protein Structure, Secondary; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid