title: The effects of laser-mediated hair removal on immunohistochemical staining properties of hair follicles.
abstract: BACKGROUND
  The mechanisms involved in laser-mediated hair removal remain unclear. One means of reducing hair growth is alteration of follicular stem cells.
  
  
  OBJECTIVE
  We sought to examine the effects of laser hair removal on the immunohistochemical staining properties of human hair follicles, including the putative stem cells of the bulge region.
  
  
  METHODS
  Treatment of unwanted axillary hair was performed on one side using an 800 nm-wavelength diode laser and on the other side using a 1064 nm-wavelength neodymium:yttrium-aluminum-garnet laser. Serial skin samples were obtained at baseline and various times after treatment and stained using immunohistochemical techniques.
  
  
  RESULTS
  Hair shafts were thermally altered, but the immunostaining properties of much of the follicle, including the bulge region, remained generally unchanged.
  
  
  CONCLUSIONS
  This study only addressed the acute immunohistochemical changes found after a single treatment using specific laser parameters.
  
  
  CONCLUSIONS
  Laser-mediated hair removal does not appear to work by frank destruction of follicular stem cells. Other mechanisms including functional alteration of these cells may underlie the clinical efficacy of the procedure.
authors.affiliation: Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0314, USA. jorringe@umich.edu
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Orringer
authors.forename: Craig
authors.initials: C
authors.lastname: Hammerberg
authors.forename: Lori
authors.initials: L
authors.lastname: Lowe
authors.forename: Sewon
authors.initials: S
authors.lastname: Kang
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Johnson
authors.forename: Ted
authors.initials: T
authors.lastname: Hamilton
authors.forename: John J
authors.initials: JJ
authors.lastname: Voorhees
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Fisher
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001365:Axilla; D004307:Dose-Response Relationship, Radiation; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D006204:Hair Removal / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D053569:Keratin-15; D007633:Keratins / Q000378:metabolism; D053685:Laser Therapy; D008875:Middle Aged; D013194:Staining and Labeling; D013234:Stem Cells / Q000378:metabolism / Q000528:radiation effects
chemical_list: D018952:Antigens, CD34; C116015:KRT15 protein, human; D053569:Keratin-15; D007633:Keratins