Conserved functions of Ikaros in vertebrate lymphocyte development: genetic evidence for distinct larval and adult phases of T cell development and two lineages of B cells in zebrafish.
Zebrafish has been advocated as an alternative animal model to study lymphocyte development, although the similarities in the genetic requirements of lymphopoiesis between fish and mammals have not yet been investigated. In this study, we examine the role of the transcription factor Ikaros in zebrafish lymphopoiesis. In fish larvae homozygous for an ikaros allele predicted to lack the C-terminal zinc fingers, T lymphopoiesis is absent; the presence of V(H)DmuJmu rearrangements in adolescent fish is delayed in mutants. In adolescent mutant fish, T cells expressing tcrb and tcrd and B cells expressing igm are formed with low efficiency and display an oligoclonal Ag receptor repertoire. By contrast, B cells expressing the igz isotype do not develop, providing genetic evidence for two separate B cell lineages in zebrafish. Thus, Ikaros appears to play similar roles in fish and mammalian lymphopoiesis.
Schorpp
Michael
M
Department of Developmental Immunology, Max-Planck Institute of Immunobiology, D-79108 Freiburg, Germany.
Bialecki
Mike
M
Diekhoff
Dagmar
D
Walderich
Brigitte
B
Odenthal
Jörg
J
Maischein
Hans-Martin
HM
Zapata
Agustin G
AG
Boehm
Thomas
T
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D019070:Cell Lineage / Q000235:genetics* / Q000276:immunology; D018389:Codon, Nonsense; D017124:Conserved Sequence; D051740:Ikaros Transcription Factor / Q000235:genetics / Q000502:physiology*; D007814:Larva; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D054388:Receptors, CCR; D019707:Receptors, Chemokine / Q000235:genetics; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D015027:Zebrafish / Q000254:growth & development* / Q000276:immunology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
C119309:CC chemokine receptor 9; D018389:Codon, Nonsense; D054388:Receptors, CCR; D019707:Receptors, Chemokine; D029961:Zebrafish Proteins; C497240:ikzf1 protein, zebrafish; D051740:Ikaros Transcription Factor