title: Spleen versus pancreas: strict control of organ interrelationship revealed by analyses of Bapx1-/- mice.
abstract: During early stages of pancreatic development, the mesenchyme that contributes to the spleen overlies the dorsal pancreatic endoderm. Here, we show that interactions between splenic mesenchyme and pancreas proceed via a highly orchestrated morphogenetic program. Disruption of morphogenesis, as occurs in the Bapx1(Nkx3.2)(-/-) embryo, results in transformation of these tissues into well-organized, ectopic gut-like structures. Bapx1 plays a crucial organizing role effecting position and separation of the spleen and pancreas to prevent this metaplastic transformation. Similar transformations occur in organ cultures employing wild-type pancreatic endoderm and spleen mesenchyme, revealing the developmental plasticity of the pancreas and that precise spatial and temporal control of tissue interactions are required for development of both organs.
authors.affiliation: Umeå Centre for Molecular Medicine, Umeå University, S-901 87, Umeå, Sweden.
authors.forename: Amir
authors.initials: A
authors.lastname: Asayesh
authors.forename: James
authors.initials: J
authors.lastname: Sharpe
authors.forename: Robert P
authors.initials: RP
authors.lastname: Watson
authors.forename: Jacob
authors.initials: J
authors.lastname: Hecksher-Sørensen
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Hastie
authors.forename: Robert E
authors.initials: RE
authors.lastname: Hill
authors.forename: Ulf
authors.initials: U
authors.lastname: Ahlgren
mesh_terms: D000818:Animals; D004707:Endoderm / Q000378:metabolism; D005260:Female; D053823:Hedgehog Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D008297:Male; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D023421:Models, Animal; D009154:Mutation; D038081:Organogenesis; D010179:Pancreas / Q000196:embryology*; D013154:Spleen / Q000196:embryology*; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D053823:Hedgehog Proteins; D018398:Homeodomain Proteins; C107965:Nkx3-2 protein, mouse; C465570:Shh protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors