title: [Study on synthesis and purification of calcium 5-formyltetrahydrofolate].
abstract: OBJECTIVE
  To establish a new approach to synthesis and purification of calcium 5-formytetrahydrofolate.
  
  
  METHODS
  Target compound was synthesized by the use of folic acid as starting material via reduction, formylation, hydrolysis and salt formation
  
  
  RESULTS
  The structure of calcium 5-formytetrahydrofolate was confirmed by UV, 1H-NMR and elemental analysis and the overall yield was 54%-59%, 14%-17% higher than the reported yield.
  
  
  CONCLUSIONS
  A convenient synthetic route was developed and it would be suitable for industrial production.
authors.affiliation: Department of Chemical Biology, School of Pharmaceutical Science, State Key laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100083, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.forename: Zhi-li
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Wu
authors.forename: Jun-yi
authors.initials: JY
authors.lastname: Liu
mesh_terms: D002118:Calcium / Q000737:chemistry*; D002955:Leucovorin / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002955:Leucovorin; D002118:Calcium