Optically specific detection of D- and L-lactic acids by a flow-injection dual biosensor system with on-line microdialysis sampling.
A flow-injection dual biosensor system with microdialysis sampling is proposed for the simultaneous determination of D-lactic and L-lactic acids. The dialysate from the microdialysis tube is delivered to a sample loop of the six-way autoinjector and then automatically injected into the flow-injection line with a dual enzyme electrode arranged in perpendicular to the flow direction. The dual enzyme electrode is constructed by hybridizing a poly(1,2-diaminobenzene) film into two sensing parts which respond selectively to D-lactic and L-lactic acids, respectively, without any cross-reactivity. The proposed flow-injection analysis method can be successfully applied to the simultaneous determination of D,L-lactic acids in alcoholic beverages.
Nanjo
Yoko
Y
Oji Scientific Instruments, Amagasaki, Hyogo, Japan.
Yano
Takayuki
T
Hayashi
Ryuzo
R
Yao
Toshio
T
D000434:Alcoholic Beverages; D015374:Biosensing Techniques; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D017022:Flow Injection Analysis / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry*; D017551:Microdialysis; D008956:Models, Chemical; D055095:Optics and Photonics; D015203:Reproducibility of Results; D013237:Stereoisomerism
D019344:Lactic Acid