[Quantitative study on copy numbers of porcine endogenous retroviruses in genome of Banna miniature swine inbred line].
OBJECTIVE
To investigate the copy numbers of porcine endogenous retroviruses in genome of Banna miniature swines for screening of donors in xenotransplantation with porcine transplants.


METHODS
The genomic DNA of peripheral blood mononuclear cells(PBMCs) of 50 Banna miniature swines in 4 inbred sublines and 5 European Large White Pigs was extracted for the detection to pol, envA, envB, envC and envA/C recombinant in PERVs with real-time quantitative/semi-quantitative PCR and normal PCR.


RESULTS
PCR products of pol, envA and envB of PERV could be obtained from all the Banna samples, and the mean amount of these products was 24.2+/-9.4,13.1+/-8.4,16.4+/-9.8 respectively and was significantly different among the inbred herds (P<0.01). The envC and envA/C were not amplified.


CONCLUSIONS
The copy numbers of pol, envA and envB genes of PERV in porcine genome were significantly different among the inbred herds of Banna miniature swines, and envC and envA/C were absent in genome of Banna Miniature Swines.
Huang
Xiao-jie
XJ
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, Beijing 100083, China.
Chen
Ping
P
Zeng
Rong
R
Ma
Kang-tao
KT
Zeng
Yang-zhi
YZ
Zhou
Chun-yan
CY
D000818:Animals; D017849:Animals, Inbred Strains; D004279:DNA, Viral / Q000235:genetics; D020077:Endogenous Retroviruses / Q000235:genetics*; D005260:Female; D015686:Gene Products, env / Q000235:genetics; D015684:Gene Products, pol / Q000235:genetics; D016678:Genome; D008297:Male; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D013552:Swine / Q000235:genetics* / Q000821:virology*; D013556:Swine, Miniature
D004279:DNA, Viral; D015686:Gene Products, env; D015684:Gene Products, pol