[Characteristics of atrazine-degrading genetically engineered microorganism (GEM) labeled with green fluorescent protein (GFP)].
The atrazine-degrading genetically engineered microorganism (GEM) was labeled by transforming a plasmid containing green fluorescent protein (GFP) gene. GFP gene could be expressed well in the cell of GEM after transforming. In the LB medium containing antibiotic, the expressing level of GFP was higher than in pure LB medium and in the basic medium. In the stable phase containing antibiotic, the expressing level of GFP was higher than in pure LB medium and in the basic medium. In the stable phase of cell growth, the expressing level of GFP was higher than in the lag phase and in the logarithmic phase. The transformation and expression of GFP gene wouldn't affect the degrading ability of GEM. The level of GFP expression was almost linear with the degrading activity. There exist two states of cells after inoculating GEM labeled with GFP into activated sludge: suspended state and adhered state to sludge floc, and the amount of suspended cells is higher than that adhered to sludge floc.
Liu
Chun
C
ESPC State Key Joint Laboratory, Department of Environmental Science and Engineering, Tsinghoa University, Beijing 100084, China.
Huang
Xia
X
Wang
Hui
H
D001280:Atrazine / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism; D012722:Sewage / Q000382:microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D006540:Herbicides; D012722:Sewage; D012989:Soil Pollutants; D049452:Green Fluorescent Proteins; D001280:Atrazine