title: [Porcine follistatin cDNA cloning and expression in Escherichia coli].
abstract: The total RNA was extracted from porcine ovary. Porcine Follistatin cDNA was cloned by RT-PCR. Complete porcine follistatin cDNA coding sequences are presented including 1038 bp of open reading frame. The purified porcine follistatin cDNA was inserted into pGEX-4T-3 vector to construct the prokaryotic fusion protein expression vector. The recombinant expression plasmid was transformed into BL21 (DE3) and expression was induced by IPTG. Protein products were detected by SDS-PAGE and confirmed by Western blotting analysis, which showed that the yield of the Follistatin cDNA was a 63kD protein expression vector. Follistatin protein was expressed in the form of glutathione-S-transferase (GST) fusion protein in E. coli.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Xin
authors.initials: X
authors.lastname: He
authors.forename: Bing
authors.initials: B
authors.lastname: Qi
authors.forename: Li-Qian
authors.initials: LQ
authors.lastname: He
authors.forename: Yong-Fu
authors.initials: YF
authors.lastname: Chen
authors.forename: Gui-Sheng
authors.initials: GS
authors.lastname: Liu
authors.forename: Qing-Xuan
authors.initials: QX
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D038681:Follistatin / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D013552:Swine
chemical_list: D038681:Follistatin; D011993:Recombinant Fusion Proteins