Redifferentiation of chondrocytes and cartilage formation under intermittent hydrostatic pressure.
Since articular cartilage is subjected to varying loads in vivo and undergoes cyclic hydrostatic pressure during periods of loading, it is hypothesized that mimicking these in vivo conditions can enhance synthesis of important matrix components during cultivation in vitro. Thus, the influence of intermittent loading during redifferentiation of chondrocytes in alginate beads, and during cartilage formation was investigated. A statistically significant increased synthesis of glycosaminoglycan and collagen type II during redifferentiation of chondrocytes embedded in alginate beads, as well as an increase in glycosaminoglycan content of tissue-engineered cartilage, was found compared to control without load. Immunohistological staining indicated qualitatively a high expression of collagen type II for both cases.
Heyland
Jan
J
Faculty of Life Science, Hamburg University of Applied Science, Hamburg, Germany.
Wiegandt
Katharina
K
Goepfert
Christiane
C
Nagel-Heyer
Stefanie
S
Ilinich
Eduard
E
Schumacher
Udo
U
Pörtner
Ralf
R
D000464:Alginates / Q000378:metabolism; D000818:Animals; D019149:Bioreactors; D002356:Cartilage / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D024043:Collagen Type II / Q000096:biosynthesis; D020723:Glucuronic Acid / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006603:Hexuronic Acids / Q000378:metabolism; D006874:Hydrostatic Pressure; D008863:Microspheres; D013552:Swine; D023822:Tissue Engineering
D000464:Alginates; D024042:Collagen Type I; D024043:Collagen Type II; D006025:Glycosaminoglycans; D006603:Hexuronic Acids; D020723:Glucuronic Acid