Apoptosis and nuclear proliferation in rat small bowel submitted to hypothermic hyperbaric oxygenation for preservation.
This study was conducted to assess apoptosis and nuclear proliferation in rat small bowel submitted to hypothermic hyperbaric oxygenation for preservation.


METHODS
Twenty two-month-old, male Wistar rats, weighing 250 g were divided into two groups: group I (n = 10), in which the small bowel was preserved for 12 hours, and group II (n = 10) in which the small bowel was preserved for 24 hours. After vascular and intraluminal perfusion, 3-cm segments were maintained in Ringer's solution at 2 degrees to 4 degrees C under normobaric conditions (groups Ia and IIa) or conditioned in a small hyperbaric metal chamber with 100% oxygen at 5.5 absolute atmospheres (groups Ib and IIb). After 12 or 24 hours, apoptotic and mitotic indices were evaluated by immunohistochemical methods.


RESULTS
The apoptotic index was significantly higher in small bowel segments in groups Ia and IIa compared with groups Ib and IIb. The mitotic index was significantly higher among group IIb.


CONCLUSIONS
Hypothermic hyperbaric oxygenation reduced intestinal epithelial apoptosis and increased nuclear proliferation during rat small bowel preservation.
Guimarães
F A G
FA
Department of Surgery, Universidade Federal de São Paulo/Escola Paulista de Medicina (UNIFESP-EPM), Rua Botucatu 740, CEP 04023-900 São Paulo-SP, Brazil. tguima@uol.com.br
Taha
M O
MO
Simões
M J
MJ
Moino
C A A
CA
Santos
I V
IV
Amador
J C
JC
Santos
R A
RA
Queiroz
R B
RB
Amaro
R R
RR
Jesus
M A S
MA
D000818:Animals; D017209:Apoptosis; D002455:Cell Division; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure*; D006931:Hyperbaric Oxygenation; D007036:Hypothermia, Induced; D007413:Intestinal Mucosa / Q000166:cytology / Q000502:physiology; D007421:Intestine, Small / Q000166:cytology* / Q000502:physiology*; D008297:Male; D008940:Mitotic Index; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar