The expression of cytochrome P-450 and cytochrome P-450 reductase genes in the simultaneous transformation of corticosteroids and phenanthrene by Cunninghamella elegans.
The expression of cytochrome P-450 and cytochrome P-450 reductase (CPR) genes in the conterminous biotransformation of corticosteroids and PAHs was studied in Cunninghamella elegans 1785/21Gp. We had previously used this strain as a microbial eucaryotic model for studying the relationship between mammalian steroid hydroxylation and the metabolization of PAHs. We reported that cytochrome P-450 reductase is involved in the biotransformaton of cortexolone and phenanthrene. RT-PCR and Northern blotting analyses indicated that the cytochrome P-450 and CPR genes appear to be inducible by both steroids and PAHs. The expression of the cytochrome P-450 gene was increased ninefold and the expression of the CPR gene increased 6.4-fold in cultures with cortexolone and/or phenanthrene in comparison with controls. We conclude that the increase in cytochrome P-450 gene expression was accompanied by an increase in cytochrome P-450 enzymatic activity levels.
Lisowska
Katarzyna
K
Department of Industrial Microbiology and Biotechnology, University of Łódź, Poland.
Szemraj
Janusz
J
Rózalska
Sylwia
S
Długoński
Jerzy
J
D001711:Biotransformation; D015152:Blotting, Northern; D003350:Cortodoxone / Q000378:metabolism*; D020093:Cunninghamella / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D009251:NADPH-Ferrihemoprotein Reductase / Q000235:genetics*; D010616:Phenanthrenes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D010616:Phenanthrenes; C031181:phenanthrene; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase; D003350:Cortodoxone