An oligodendroglial progenitor cell line FBD-102b possibly secretes a radial glia-inducing factor.
Interactions between oligodendroglial and astroglial lineages during development are still unclear. In this study, FBD-102b, derived from p53-/- fetal brains, was characterized as an oligodendroglial progenitor cell (OPC) line. In co-culture with 102b cells, cells of an astrocyte progenitor cell line, FBD-104, elongated monopolar processes, like radial glia, and actively proliferated. Conditioned medium of 102b cells also induced the process elongation that was associated with down-regulation of GFAP and up-regulation of vimentin and nestin. These results suggested that OPCs secrete a radial glia-inducing factor that might maintain the radial glial processes to use as scaffolds of migration.
Horiuchi
Makoto
M
Department of Biological Science and Technology, and Tissue Engineering Research Center, Tokyo University of Science, 2641 Yamazaki, Noda City, Chiba 278-8510, Japan.
Tomooka
Yasuhiro
Y
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000502:physiology; D015153:Blotting, Western / Q000379:methods; D001921:Brain; D001973:Bromodeoxyuridine / Q000378:metabolism; D002460:Cell Line; D019070:Cell Lineage; D018920:Coculture Techniques / Q000379:methods; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004622:Embryo, Mammalian; D015870:Gene Expression / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D007150:Immunohistochemistry / Q000379:methods; D008222:Lymphokines / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D004676:Myelin Basic Protein / Q000378:metabolism; D009836:Oligodendroglia / Q000187:drug effects / Q000378:metabolism*; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency
D017077:Culture Media, Conditioned; D005904:Glial Fibrillary Acidic Protein; D008222:Lymphokines; D004676:Myelin Basic Protein; D016159:Tumor Suppressor Protein p53; C032916:glia cell stimulating factor; D049452:Green Fluorescent Proteins; D001973:Bromodeoxyuridine