Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: intracellular concentration decrease in NAD and increase in cyclic ADP-ribose.
ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastomaxglioma NGPM1-27 hybrid cells was measured by monitoring [(3)H] cyclic ADP-ribose (cADPR) formation from [(3)H] NAD(+). Bradykinin (BK) at 100nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300nM BK to living NGPM1-27 cells decreased NAD(+) to 78% of the prestimulation level at 30s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B(2) BK receptors.
Higashida
Haruhiro
H
Department of Biophysical Genetics, Kanazawa University Graduate School of Medicine, 13-1 Takara, Kanazawa, Ishikawa 920-8640, Japan. haruhiro@med.kanazawa-u.ac.jp
Salmina
Alla
A
Hashii
Minako
M
Yokoyama
Shigeru
S
Zhang
Jia-Sheng
JS
Noda
Mami
M
Zhong
Zen-Guo
ZG
Jin
Duo
D
D036541:ADP-ribosyl Cyclase / Q000378:metabolism*; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D036563:Cyclic ADP-Ribose / Q000378:metabolism*; D006822:Hybrid Cells / Q000166:cytology / Q000187:drug effects; D051379:Mice; D009243:NAD / Q000378:metabolism*; D009447:Neuroblastoma; D051381:Rats; D015290:Second Messenger Systems / Q000502:physiology; D014665:Vasodilator Agents / Q000494:pharmacology*
D014665:Vasodilator Agents; D009243:NAD; D036563:Cyclic ADP-Ribose; D036541:ADP-ribosyl Cyclase; D001920:Bradykinin