Usefulness of dura mater in providing DNA samples for identifying cadavers.
We examined the usefulness of the dura mater in identifying human remains. Dura mater was collected from 50 cadavers, including drowned, charred, and mummified remains. The STR genotype using the AmpFlSTR Identifiler Kit could be typed at 15 STR and amelogenin loci in 30 samples of 33 cases. Furthermore, the ABO genotype and amelogenin using gel-based methods could be typed in 44 samples of 50 cases. In cases with successful typing of STR, ABO-DNA, and amelogenin, the longest time after death was from 12 to 26 days in a drowned body. The minimum quantity of dura mater required for DNA extraction was about 2.5 mg, dried and fixed by ethanol, in a cadaver 15 h after death. The state of the DNA from the dura mater from the calvaria may be better than that from the basis cranii interna. We found that DNA from dura mater is one of the most useful samples for forensic identification.
Motani
Hisako
H
Department of Legal Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. hms1466@faculty.chiba-u.jp
Sakurada
Koichi
K
Akutsu
Tomoko
T
Ikegaya
Hiroshi
H
Hayakawa
Mutsumi
M
Sato
Yayoi
Y
Yajima
Daisuke
D
Sato
Kaoru
K
Kobayashi
Kazuhiro
K
Iwase
Hirotaro
H
D000017:ABO Blood-Group System / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D053523:Amelogenin; D002102:Cadaver; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D003746:Dental Enamel Proteins / Q000235:genetics; D004388:Dura Mater / Q000473:pathology*; D005260:Female; D018732:Forensic Anthropology / Q000379:methods*; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D019849:Sex Determination Processes; D020080:Tandem Repeat Sequences
D000017:ABO Blood-Group System; D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA