title: Plasma cytokines as markers of aseptic prosthesis loosening.
abstract: The proinflammatory cytokines IL-1beta, IL-8, and TNF-alpha play a major role in the process of bone resorption during aseptic loosening of large joint prostheses. These cytokines secreted locally during bone resorption in aseptic loosening may enter peripheral circulation. Increased concentration of IL-1gamma, IL-8, and TNF-alpha in peripheral circulation may indicate aseptic loosening. We determined whether bone resorption could be verified by cytokine presence in plasma. We recruited 50 patients with aseptic prosthesis loosening, 50 with stable prostheses, 50 with osteoarthritis, and 50 healthy individuals. Cytokine levels were determined in plasma by ELISA tests. Patients with prosthesis loosening had higher plasma levels (IL-10, 3.7 +/- 5.5 pg/mL; IL-8, 14.7 +/- 9 pg/mL; TNF-alpha, 32.7 +/-+/- 32.4 pg/mL) than patients with stable prostheses (IL-1beta, 1.5 +/- 2 pg/mL; IL-8, 8.1 +/- 4.7 pg/mL; TNF-alpha, 22.9 +/- 18.7 pg/mL), patients with osteoarthritis (IL-1beta, 0.7 +/- 1.1 pg/mL; IL-8, 5.8 +/- 3.8 pg/mL; TNF-alpha, 9.8 +/- 7.7 pg/mL) and healthy individuals (IL-1beta, 0.7 +/- 1.1 pg/mL; IL-8, 4.2 +/- 1.3 pg/mL; TNF-alpha, 3.9 +/- 3.9 pg/mL). Our data suggest elevated plasma levels of proinflammatory cytokines may be useful as markers of bone resorption in the laboratory diagnosis of prosthesis loosening.
authors.affiliation: Croatian Institute of Transfusion Medicine, Zagreb, Croatia. zeljka.hundric-haspl@hztm.htnet.hr
authors.forename: Zeljka
authors.initials: Z
authors.lastname: Hundrić-Haspl
authors.forename: Marko
authors.initials: M
authors.lastname: Pecina
authors.forename: Miroslav
authors.initials: M
authors.lastname: Haspl
authors.forename: Maja
authors.initials: M
authors.lastname: Tomicic
authors.forename: Irena
authors.initials: I
authors.lastname: Jukic
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D007595:Joint Prosthesis; D008297:Male; D008875:Middle Aged; D011475:Prosthesis Failure; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha