title: Tissue microarray analysis of FAS, Bcl-2, Bcl-x, ER, PgR, Hsp60, p53 and Her2-neu in breast carcinoma.
abstract: BACKGROUND
  The aim of this study was to detect immunohistochemical markers in breast carcinoma by means of tissue microarray analysis (TMA) and to associate their expressions with clinicopathological features and prognosis. Fatty acid synthase, bcl-2, bcl-x, p53, estrogen and progesterone receptors, heat shock protein 60 and Her2-neu (c-erbB-2) were evaluated in a group of 149 breast carcinoma patients with a 5-year follow-up period.
  
  
  METHODS
  TMA blocks were made by using duplicate 0.6-mm diameter tissue cores from each paraffin block.
  
  
  RESULTS
  Statistical analysis revealed that tumor stage (p=0.003) and node status (p=0.001) were the only two prognostic markers of disease-free survival. Moreover, FAS and bcl-x showed an independent effect on recurrence (p=0.005). The node status was the only marker of overall survival (p=0.05).
  
  
  CONCLUSIONS
  Our data confirmed recent reports associating the stage of disease, FAS and Bcl-x expressions with recurrence and outcome. These data demonstrated that TMA is an effective substitute for conventional histochemical-immunohistochemical techniques.
authors.affiliation: Department of Experimental Medicine and Pathology, School of Specialization in Anatomic Pathology, University of Rome La Sapienza, Rome, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Sebastiani
authors.forename: Claudio
authors.initials: C
authors.lastname: Botti
authors.forename: Ugo
authors.initials: U
authors.lastname: Di Tondo
authors.forename: Paolo
authors.initials: P
authors.lastname: Visca
authors.forename: Laura
authors.initials: L
authors.lastname: Pizzuti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Santeusanio
authors.forename: Piero Luigi
authors.initials: PL
authors.lastname: Alo
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D018834:Chaperonin 60 / Q000032:analysis / Q000096:biosynthesis; D064429:Fatty Acid Synthases / Q000032:analysis / Q000096:biosynthesis; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis*; D040081:Protein Array Analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000096:biosynthesis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000032:analysis / Q000096:biosynthesis; D011980:Receptors, Progesterone / Q000032:analysis / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis; D051020:bcl-X Protein / Q000032:analysis / Q000096:biosynthesis
chemical_list: D018834:Chaperonin 60; D064429:Fatty Acid Synthases; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D016159:Tumor Suppressor Protein p53; D051020:bcl-X Protein; C495901:TP53 protein, human