DQA1 and DQB1 association and nasal polyposis.
OBJECTIVE
The aim of this study was to determine the contribution of the human major histocompatibility complex (HLA)-DQA1, -DQB1, and TNFalpha genes with simple nasal polyposis.


METHODS
A comparative case-control study with 31 patients and 151 controls was performed. HLA-DQA1, -DQB1, and TNFalpha -238 promoter position loci were typed by polymerase chain reaction (PCR) and sequence specific oligonucleotide probes (SSOPs). TNFalpha -308 promoter position was determined by PCR and digestion with NcoI restriction enzyme.


RESULTS
The allele HLA-DQA1*0201 (P(c) = 0.019) had an etiologic fraction (EF) of 17%, whereas 13% EF was found for the haplotype HLA-DQA1*0201-DQB1*0201 (P = 0.016). Analysis of -DQB1 and TNFalpha promoter did not show significant differences between cases and controls.


CONCLUSIONS
HLA-DQA1*0201-DQB1*0201 haplotype is involved in susceptibility, conferring 5.53 times more risk of developing this disease.


METHODS
B-2b.
Fajardo-Dolci
Germán
G
Clinical Division of Otorhynolaryngology, Hospital General Dr. Manuel Gea González, SSA. Calz. de Tlalpan 4800, Col. Toriello Guerra, 14000 México, DF México. fajardo.german@gmail.com
Solorio-Abreu
Jesús
J
Romero-Alvarez
Julio César
JC
Zavaleta-Villa
Beatríz
B
Cerezo-Camacho
Oscar
O
Jiménez-Lucio
Rocío
R
Olivo-Díaz
Angélica
A
D016022:Case-Control Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006239:Haplotypes; D006801:Humans; D009298:Nasal Polyps / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; C057615:HLA-DQbeta antigen; D014409:Tumor Necrosis Factor-alpha