title: [Proliferation and undifferentiation of spermatogonial stem cells in vitro].
abstract: OBJECTIVE
  To investigate the fluences on the proliferation and undifferentiation of spermatogonial stem cells in vitro.
  
  
  METHODS
  Mouse bone marrow stromal cells were subjected to primary culture; when the cells grew up and extended around, they were treated with mitomycin C. And then, to the bone marrow stromal cells were added spermatogonial stem cells for co-culture. The ambient temperature was 37 degrees C.
  
  
  RESULTS
  The spermatogonial stem cells increased in number obviously when cultured for 2-4 days, they could be single, paired or clustered, while the differentiated cells began to die; when cultured for 4-8 days, the cells proliferated not in a distinct manner, they seemed to be stable; but at 10 days, the cells began to grow, especially when cultured for 15 days, the cells had the characteristic structure of the spermatogonial stem cell; when cultured continuously for 25 days, the cells had the same morphology and very clear intercellular bridges.
  
  
  CONCLUSIONS
  Spermatogonial stem cells can proliferate and continue to be undifferentiated on the bone marrow stromal cells when the ambient temperature is 37 degrees C.
authors.affiliation: Department of Histology and Embryology, Luzhou Medical College, Luzhou 646000, China.
authors.forename: Fu-cui
authors.initials: FC
authors.lastname: Xu
authors.forename: Shao-hua
authors.initials: SH
authors.lastname: Wu
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.forename: Xin-ming
authors.initials: XM
authors.lastname: Mei
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D008297:Male; D051379:Mice; D016685:Mitomycin / Q000494:pharmacology; D013093:Spermatogonia / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology; D013696:Temperature
chemical_list: D016685:Mitomycin