title: [Comparison of two types of diagnostic test detecting antibodies against Borrelia burgdorferi: EIA based on one genospecies antigens and ELISA based on recombinant antigens].
abstract: The aim of the study was to compare the results of ELISA diagnostic kit detecting antibodies against B. burgdorferi based on combination of three genospecies recombinant and EIA kits based on one of three genospecies. Sera of 351 forest workers were evaluated with ELISA kit (Recombinant antigen, IgG). Seropositive samples were tested with EIA kits based on B. burgdorferi s.s., B. garinii, B. afzelii antigens. Diagnostic kits based on combination of antigens of three genospecies more often detect antibodies against B. burgdorferi and are more usefull as screening tests, in comparison with those based on one genospecies. Among diagnostic kits based on one genospecies, the most sesnitive in detection of antibodies against B. burgdorferi are those based on B. afzelii.
authors.affiliation: Klinika Chorób Zakaźnych i Neuroinfekcji, Akademii Medycznej w Białymstoku.
authors.forename: Joanna
authors.initials: J
authors.lastname: Zajkowska
authors.forename: Maciej
authors.initials: M
authors.lastname: Kondrusik
authors.forename: Sambor
authors.initials: S
authors.lastname: Grygorczuk
authors.forename: Sławomir
authors.initials: S
authors.lastname: Pancewicz
authors.forename: Renata
authors.initials: R
authors.lastname: Swierzbińska
authors.forename: Iwona
authors.initials: I
authors.lastname: Sienkiewic
authors.forename: Teresa
authors.initials: T
authors.lastname: Hermanowska-Szpakowicz
mesh_terms: D000382:Agricultural Workers' Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000145:classification*; D015748:Borrelia burgdorferi Group / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016468:Forestry; D006801:Humans; D007124:Immunoenzyme Techniques; D008193:Lyme Disease / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D008297:Male; D011044:Poland; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial