Synthesis of poly d(G-C) oligonucleotides.
Model sequences for evaluation of the GC dimer sequence repetition on synthesis success were prepared and analyzed by HPLC. Contiguous d(G-C) or d(C-G) sequences have a deleterious effect on DNA oligonucleotide synthesis. The critical number seems to be about 6 GCs in a row. If the GCs are separated by other nucleotides, the effect is not as severe.
Hachmann
John P
JP
Illumina, Inc., 9885 Towne Centre Dr., San Diego, CA 92130, USA.
Lebl
Michal
M
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D019281:Dimerization; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012680:Sensitivity and Specificity
D009841:Oligonucleotides; D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG)