title: SHV-5 extended-spectrum beta-lactamases in clinical isolates of Escherichia coli in Malaysia.
abstract: Escherichia coli isolates resistant to ceftazidime isolated in the University Malaya Medical Center (UMMC) Kuala Lumpur, Malaysia, between the years 1998 and 2000 were studied for extended-spectrum beta-lactamase (ESBL) production. All strains were analysed phenotypically and genotypically and found to be ESBL-producing organisms harbouring SHV-5 beta-lactamase. This was confirmed by PCR-SSCP and nucleotide sequencing of the blaSHV amplified gene. As there was no evidence of ESBL activity in E. coli prior to this, coupled with the fact that there was a predominance of SHV-5 beta-lactamases in Klebsiella pneumoniae isolates in UMMC, we postulate that the E. coli obtained the SHV-5 beta-lactamase genes by plasmid transfer from the ESBL-producing K. pneumoniae.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: G
authors.initials: G
authors.lastname: Subramaniam
authors.forename: S
authors.initials: S
authors.lastname: Palasubramaniam
authors.forename: P
authors.initials: P
authors.lastname: Navaratnam
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D004269:DNA, Bacterial; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D022761:Gene Transfer, Horizontal; D005838:Genotype; D006801:Humans; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008296:Malaysia; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002442:Ceftazidime; C117193:beta-lactamase SHV-5; D001618:beta-Lactamases