Extracellular matrix remodeling--methods to quantify cell-matrix interactions.
Tissue turnover during wound healing, regeneration or integration of biomedical materials depends on the rate and extent of materials trafficking into and out of cells involved in extracellular matrix (ECM) remodeling. To exploit these processes, we report the first model for matrix trafficking in which these issues are quantitatively assessed for cells grown on both native collagen (normal tissue) and denatured collagen (wound state) substrates. Human fibroblasts more rapidly remodeled denatured versus normal collagen type I to form new ECM. Fluxes to and from the cells from the collagen substrates and the formation of new ECM were quantified using radioactively labeled substrates. The model can be employed for the systematic and quantitative study of the impact of a broad range of physiological factors and disease states on tissue remodeling, integrating extracellular matrix structures and cell biology.
Abraham
Leah C
LC
Department of Chemical and Biological Engineering, and Bioengineering and Biotechnology Center, Tufts University, Medford, MA 02155, USA.
Dice
J Fred
JF
Finn
Patrick F
PF
Mesires
Nicholas T
NT
Lee
Kyongbum
K
Kaplan
David L
DL
D002450:Cell Communication / Q000502:physiology*; D002460:Cell Line; D003094:Collagen / Q000737:chemistry*; D003238:Connective Tissue / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological
D003094:Collagen