title: Cephalopod tropomyosins: identification as major allergens and molecular cloning.
abstract: Heated extracts prepared from the mantle muscles (for decapods) or leg muscles (for octapods) of nine species of cephalopods were shown to be all reactive with serum IgE in crustacean-allergic patients. No marked difference in the reactivity with IgE was recognized among the cephalopods, suggesting that they are almost equally allergenic. Immunoblotting and inhibition immunoblotting data revealed that the major allergen is tropomyosin in common with the nine species of cephalopods and that the cephalopod tropomyosins are cross-reactive with one another and also with crustacean tropomyosins. Molecular cloning experiments first elucidated the primary structures of tropomyosins from five species of cephalopods. The cephalopod tropomyosins show high sequence identity (more than 92% identity) with one another, being the molecular basis for their cross-reactivity. Although the sequence identity between cephalopod and crustacean topomyosins is only about 63-64%, some of the IgE-binding epitopes proposed for brown shrimp Penaeus aztecus tropomyosin (Pen a 1) are well conserved in the cephalopod tropomyosins, supporting the cross-reactivity between cephalopod and crustacean tropomyosins.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan-4, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Motoyama
authors.forename: S
authors.initials: S
authors.lastname: Ishizaki
authors.forename: Y
authors.initials: Y
authors.lastname: Nagashima
authors.forename: K
authors.initials: K
authors.lastname: Shiomi
mesh_terms: D000485:Allergens; D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D049830:Cephalopoda / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005512:Food Hypersensitivity / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D016415:Sequence Alignment; D013045:Species Specificity; D014335:Tropomyosin / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000485:Allergens; D060829:Arthropod Proteins; D018076:DNA, Complementary; D011506:Proteins; D014335:Tropomyosin; C058449:shrimp allergens Sa; D007073:Immunoglobulin E