title: Neoplaether, a new cytotoxic and antifungal endophyte metabolite from Neoplaconema napellum IFB-E016.
abstract: A new diphenyl ether, named neoplaether, together with five known compounds monomethylsulochrin, physcion, helvolic acid, ergosterol and ergosterol peroxide, was isolated from the culture of Neoplaconema napellum IFB-E016, an endophytic fungus residing in the healthy leaves of Hopea hainanensis. The structure of neoplaether was elucidated by a correlative interpretation of its infrared spectrometry, mass spectroscopy and nuclear magnetic resonance spectra, and confirmed by its single-crystal X-ray diffraction analysis. Neoplaether exhibited significant cytotoxic activity against the human nasopharyngeal epidermoid tumor KB cell line, with an IC(50) value of 13.0 microg mL(-1), comparable to that of 5-fluorouracil (2.5 microg mL(-1)) co-assayed as a positive reference. In addition, it showed antifungal activity against Candida albicans, with a minimal inhibitory concentration value of 6.2 microg mL(-1) (amphotericin as a positive control had a value of 1.5 microg mL(-1)).
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, China.
authors.forename: Feng W
authors.initials: FW
authors.lastname: Wang
authors.forename: Yong H
authors.initials: YH
authors.lastname: Ye
authors.forename: Jing R
authors.initials: JR
authors.lastname: Chen
authors.forename: Xiao T
authors.initials: XT
authors.lastname: Wang
authors.forename: Hai L
authors.initials: HL
authors.lastname: Zhu
authors.forename: Yong C
authors.initials: YC
authors.lastname: Song
authors.forename: Ren X
authors.initials: RX
authors.lastname: Tan
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D018360:Crystallography, X-Ray; D020448:Ericales / Q000382:microbiology*; D006801:Humans; D007624:KB Cells; D010647:Phenyl Ethers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000382:microbiology
chemical_list: D000903:Antibiotics, Antineoplastic; D000935:Antifungal Agents; D010647:Phenyl Ethers; C031538:phenyl ether