An essential GT motif in the lamin A promoter mediates activation by CREB-binding protein.
Lamin A is an important component of nuclear architecture in mammalian cells. Mutations in the human lamin A gene lead to highly degenerative disorders that affect specific tissues. In studies directed towards understanding the mode of regulation of the lamin A promoter, we have identified an essential GT motif at -55 position by reporter gene assays and mutational analysis. Binding of this sequence to Sp transcription factors has been observed in electrophoretic mobility shift assays and by chromatin immunoprecipitation studies. Further functional analysis by co-expression of recombinant proteins and ChIP assays has shown an important regulatory role for CREB-binding protein in promoter activation, which is mediated by the GT motif.
Janaki Ramaiah
M
M
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, Andhra Pradesh, India.
Parnaik
Veena K
VK
D000818:Animals; D001483:Base Sequence; D050882:CREB-Binding Protein / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D018900:Dinucleotide Repeats / Q000502:physiology*; D005786:Gene Expression Regulation; D006147:Guanine / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D034904:Lamin Type A / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D013941:Thymine
D034904:Lamin Type A; D006147:Guanine; D050882:CREB-Binding Protein; D013941:Thymine