Determination of arbidol in human plasma by LC-ESI-MS.
A sensitive, specific and accurate method for determination of arbidol in human plasma was developed. Arbidol and internal standard were extracted from plasma samples by liquid-liquid extraction with diethyl ether. The chromatographic separation was accomplished on a Shiseido C18 3 microm analytical column (100 mm x 2.0 mm i.d.) at a flow rate of 0.3 mL/min isocratically. Detection was performed on a single quadrupole mass spectrometer by selected ion monitoring (SIM) mode via electrospray ionization (ESI) source. The method had a chromatographic run time of 6 min and a good linear relationship over the range 1-1000 ng/mL. The limit of quantitation for arbidol in plasma was 1 ng/mL. The intra-day and inter-day precision (R.S.D.%) was lower than 7% and accuracy ranged from 95 to 105%. The proposed method enables unambiguous identification and quantification of arbidol in vivo and has been successfully applied to study the pharmacokinetics of arbidol in healthy male Chinese volunteers.
Liu
Xiao
X
Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China.
Huang
Yu-Wei
YW
Li
Juan
J
Li
Xiao-Bo
XB
Bi
Kai-Shun
KS
Chen
Xiao-Hui
XH
D000328:Adult; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005615:Freezing; D006801:Humans; D007202:Indicators and Reagents; D007211:Indoles / Q000097:blood* / Q000493:pharmacokinetics; D007369:Interferon Inducers / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature; D013440:Sulfides
D007202:Indicators and Reagents; D007211:Indoles; D007369:Interferon Inducers; C086979:umifenovir; D013440:Sulfides