title: Effects of etamsylate on equine platelets: in vitro and in vivo studies.
abstract: The aim of this study was to investigate whether etamsylate produces equine platelet activation. In vitro and in vivo studies were designed in which seven and eight adult healthy horses were included, respectively. In the in vitro study, citrated blood was incubated with different concentrations of etamsylate, and P-selectin expression and annexin V binding were determined by flow cytometry. In the in vivo study, blood was collected before and 1 and 2h after IV administration of etamsylate, and P-selectin expression was evaluated. In the in vitro study, a significant increase in P-selectin expression, leukocyte-platelet aggregate formation and annexin V binding were observed. In the in vivo study, a marked increase in P-selectin expression and heterotypic aggregate formation was seen in two and five horses, respectively, although no significant differences were detected when analyzing results from all the animals together. The results of the in vitro study indicate that etamsylate produces a pre-activation state in equine platelets, but this fact could be confirmed by the in vivo study.
authors.affiliation: Servei de Medicina Interna Equina, Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Dídac
authors.initials: D
authors.lastname: Segura
authors.forename: Luis
authors.initials: L
authors.lastname: Monreal
authors.forename: Sílvia
authors.initials: S
authors.lastname: Pérez-Pujol
authors.forename: Antonio
authors.initials: A
authors.lastname: Alonso
authors.forename: Maribel
authors.initials: M
authors.lastname: Díaz-Ricart
authors.forename: Rosa
authors.initials: R
authors.lastname: Brugués
authors.forename: Antonio
authors.initials: A
authors.lastname: Ordinas
authors.forename: Ginés
authors.initials: G
authors.lastname: Escolar
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004979:Ethamsylate / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary; D006490:Hemostatics / Q000494:pharmacology*; D006736:Horses / Q000097:blood* / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D015539:Platelet Activation / Q000187:drug effects*; D010976:Platelet Count / Q000662:veterinary
chemical_list: D017304:Annexin A5; D006490:Hemostatics; D019007:P-Selectin; D004979:Ethamsylate