[Effect of maixinkang capsule on Ca2+ and mitochondrial membrane potential in liver cells of ApoE(-/-) mice].
OBJECTIVE
To observe the effect of Maixinkang Capsule (MXK) on Ca2t concentration and mitochondrial membrane potential in liver cells of ApoE(-/-) mice.


METHODS
Liver cells from ApoE(-/-) mice were separated using collagenase digestive method. After the primary cells were cultured for 8 days in vitro, the concentration of 10% MXK contained rat's serum was added into the culture fluid. The Ca2+ concentration and mitochondrial membrane potential in liver cells after 48-hr culture were measured by confocal laser scanning microscopy with Flou-3 and Jc-1 as probes.


RESULTS
MXK could decrease Ca2+ concentration in liver cells, which was significantly different to that in the control group (P < 0.01). Meanwhile, MXK could significantly improve mitochondrial membrane potential in liver cells (P < 0.01). There was no obvious dose-effect relationship shown in both effects of MXK.


CONCLUSIONS
MXK can decrease Ca2+ concentration and improve the mitochondrial membrane potential in liver cells of ApoE(-/-) mice so as to regulate the lipids and prevent the occurrence and development of hyperlipemia and atherosclerosis.
Zheng
Guang-juan
GJ
Basal Medicine College, Shandong University of Traditional Chinese Medicine, Jinan. zgj640@sohu.com
Zhang
Wen-gao
WG
Zhu
Qing-jun
QJ
D000818:Animals; D000831:Animals, Newborn; D001057:Apolipoproteins E / Q000235:genetics*; D015220:Calcium Channels / Q000187:drug effects*; D022781:Hepatocytes / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D018345:Mice, Knockout; D051336:Mitochondrial Membranes / Q000502:physiology*
D001057:Apolipoproteins E; D015220:Calcium Channels