An unusual extended DNA loop attachment region is located in the human dystrophin gene.
We have found and characterized an unusual extended area of DNA association with the nuclear matrix in the human dystrophin gene. This extended DNA loop anchorage region (LAR) has been mapped and characterized using a variety of biochemical and microscopy techniques. It spans approximately 200 kbp at chromosomal locations 950-1,150 Kb downstream to the beginning of the first exon of the dystrophin gene Dp427m and covers a part of the intron 43, exon 44, and most of intron 44. The extended LAR harbors the major recombination hot spot of the dystrophin gene and a replication origin. We propose a model where DNA topoisomerase II-mediated cleavage at the nuclear matrix may enhance recombination events within this extended LAR.
Iarovaia
Olga V
OV
Institute of Gene Biology RAS, Moscow, Russia.
Borounova
Victoria
V
Vassetzky
Yegor S
YS
Razin
Sergey V
SV
D002874:Chromosome Mapping; D004247:DNA / Q000737:chemistry*; D016189:Dystrophin / Q000235:genetics*; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D045170:Matrix Attachment Regions / Q000235:genetics*; D015530:Nuclear Matrix / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D059005:Topoisomerase II Inhibitors; D014407:Tumor Cells, Cultured
D016189:Dystrophin; D059005:Topoisomerase II Inhibitors; D004247:DNA