Comparative inflammatory properties of staphylococcal superantigenic enterotoxins SEA and SEG: implications for septic shock.
The severity of Staphylococcus aureus sepsis is positively associated with staphylococcal enterotoxin A (SEA) and negatively associated with the enterotoxin gene cluster (egc), which encodes five staphylococcal enterotoxins. We postulated that the variable, clinical severity of S. aureus sepsis might be a result of differences in the inflammatory properties of staphylococcal superantigens. We therefore compared the inflammatory properties of SEA with those of staphylococcal entérotoxin G (SEG), a member of the five egc superantigens. We found that SEA and SEG had similar superantigenic properties, as they induced CD69 expression on T lymphocytes and selective expansion of Vbeta subpopulations. Contrary to SEG, however, SEA induced a strong proinflammatory/Th1 response, including TNF-alpha and MIP-1alpha production. These results suggest that the association of SEA with the severity of S. aureus septic shock, characterized by a deleterious, inflammatory cascade, may be explained partly by the specific proinflammatory properties of this superantigen.
Dauwalder
Olivier
O
Université Claude Bernard, INSERM E230, IFR-62, Lyon, France.
Thomas
Damien
D
Ferry
Tristan
T
Debard
Anne-Lise
AL
Badiou
Cédric
C
Vandenesch
François
F
Etienne
Jerome
J
Lina
Gerard
G
Monneret
Guillaume
G
D015703:Antigens, CD / Q000096:biosynthesis / Q000187:drug effects; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000187:drug effects; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000276:immunology* / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000276:immunology; D037181:Lectins, C-Type; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis; D012772:Shock, Septic / Q000276:immunology*; D013329:Structure-Activity Relationship; D018089:Superantigens / Q000276:immunology* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000187:drug effects; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D004768:Enterotoxins; D037181:Lectins, C-Type; D019402:Macrophage Inflammatory Proteins; D018089:Superantigens; D014409:Tumor Necrosis Factor-alpha; D000099192:CD69 Antigens; C419397:enterotoxin G, staphylococcal; C026066:enterotoxin A, Staphylococcal