title: Comparative trial of different anti-bacterial combinations with propolis and ciprofloxacin on Pseudomonas keratitis in rabbits.
abstract: The aim of the current study was to evaluate the effects of five different treatment combinations to find out whether propolis could be an alternative or an adjunctive treatment, in experimental Pseudomonas aeruginosa keratitis. Intrastromal P. aeruginosa strains were given to both eyes of 20 young New Zealand white rabbits. The rabbits were randomly divided equally into five treatment groups; ciprofloxacin and dexamethasone drops (C+D), ciprofloxacin drop (C), ciprofloxacin and propolis drops (C+P), propolis drop (P), 3% ethanol drop (control), respectively. Directly before the first treatment and 108 h after inoculation, the eyes were examined by slit lamp to assess the corneal opacity and rabbits were sacrificed for bacterial count. The mean corneal opacity scores and the mean bacterial counts log cfu/ml were significantly different in the treatment groups (P=0.001; ANOVA). According to post hoc tests for both the mean bacterial counts and corneal opacity scores, C+D, C, C+P groups were found to be statistically the same (P>0.05), and although the P group had significantly better scores than the control group it did not reach the scores of the rest of the treatment groups (P<0.01). We conclude that propolis may be a useful adjunctive agent but should not be regarded as a replacement for traditional antibiotic therapy for P. aeruginosa keratitis in rabbits.
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, School of Medicine, Mustafa Kemal University, Antakya/Hatay, Turkey. onlenyusuf@yahoo.com
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Onlen
authors.forename: Cengaver
authors.initials: C
authors.lastname: Tamer
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Oksuz
authors.forename: Nizami
authors.initials: N
authors.lastname: Duran
authors.forename: Mehmet Enes
authors.initials: ME
authors.lastname: Altug
authors.forename: Selvinaz
authors.initials: S
authors.lastname: Yakan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D002939:Ciprofloxacin / Q000627:therapeutic use*; D015169:Colony Count, Microbial; D003318:Corneal Opacity; D003907:Dexamethasone / Q000627:therapeutic use; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D000431:Ethanol / Q000627:therapeutic use; D005123:Eye / Q000382:microbiology; D007634:Keratitis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D011429:Propolis / Q000627:therapeutic use*; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000473:pathology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D011817:Rabbits; D011897:Random Allocation
chemical_list: D000900:Anti-Bacterial Agents; D000431:Ethanol; D002939:Ciprofloxacin; D003907:Dexamethasone; D011429:Propolis