Granulocyte antibodies in Korean neonates with neutropenia.
Neonatal alloimmune neutropenia (NAN) is a disease that can cause severe and prolonged neutropenia in neonates. However, no report is available on the incidence of granulocyte antibody in neonates, the target antigen of this antibody, and the estimated incidence of NAN in Korea. Among a total of 856 neonates admitted to a neonatal intensive care unit (NICU) over a five year period, a total of 105 neonates with neutropenia were enrolled in this study. Positive reactions were observed in the sera of six neonates (5.7%, 6/105) by mixed passive hemagglutination assay (MPHA). To confirm the presence of NAN, MPHA and granulocyte antigen typing (HNA-1a, -1b, -2a, -4a, and -5a) were performed on neonatal and maternal blood. To differentiate granulocyte antibody and HLA antibody, MPHA was also performed using HLA antibody adsorbed serum. We confirmed three cases (2.9%, 3/105) of NAN among neonates with neutropenia in which granulocyte antibody specificities (two anti-HNA-1b and one anti-HNA-1a) and fetomaternal granulocyte antigen mismatches were identified. In this study, the estimated incidence of NAN was 0.35% (3/856) among neonates admitted to NICUs in Korea.
Han
Tae Hee
TH
Department of Laboratory Medicine, Inje University Sanggye Paik Hospital, Nowon-gu, Seoul, Korea. kscosby@sanggyepaik.ac.kr
Chey
Myoung-Jae
MJ
Han
Kyou Sup
KS
D000918:Antibody Specificity / Q000276:immunology; D005838:Genotype; D006098:Granulocytes / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal / Q000706:statistics & numerical data; D007518:Isoantibodies / Q000276:immunology*; D007519:Isoantigens / Q000235:genetics / Q000276:immunology; D007723:Korea; D009503:Neutropenia / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods
D006680:HLA Antigens; D007518:Isoantibodies; D007519:Isoantigens; C067704:neutrophil-specific antigen NA1, human