title: Inhibitory effects of Orostachys japonicus extracts on the formation of N-nitrosodimethylamine.
abstract: In Korea, Orostachys japonicus has been used traditionally as a drug and health food. The aim of this study was to investigate possible inhibitory effects of O. japonicus extracts on the formation of N-nitrosodimethylamines (NDMA). Chloroform extraction was the most effective method for recovering the highest number of phenolic compounds and flavonoids; in these extracts the greatest nitrite-scavenging activity and inhibition of NDMA formation occurred at pH 2.5. The chloroform extract was separated into 10 fractions (J1-J10); fraction J4 inhibited NDMA formation by 90.1 +/- 0.4%. This fraction was then separated into five subfractions (J4-1-J4-5) using a silica gel column. Subfractions J4-2 [(+)-catechin] and J4-4 (3,4-dihdroxybenzoic acid) inhibited NDMA formation by 89.5 +/- 0.9 and 77.6 +/- 0.8%, respectively.
authors.affiliation: Department of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, South Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Mi Ja
authors.initials: MJ
authors.lastname: Chung
authors.forename: Woo Duck
authors.initials: WD
authors.lastname: Seo
authors.forename: Jung Hye
authors.initials: JH
authors.lastname: Shin
authors.forename: Mi Yae
authors.initials: MY
authors.lastname: Shon
authors.forename: Nak Ju
authors.initials: NJ
authors.lastname: Sung
mesh_terms: D002273:Carcinogens / Q000037:antagonists & inhibitors* / Q000138:chemical synthesis; D002725:Chloroform; D029627:Crassulaceae / Q000737:chemistry*; D004128:Dimethylnitrosamine; D005419:Flavonoids / Q000494:pharmacology; D009573:Nitrites / Q000737:chemistry; D009602:Nitrosamines / Q000037:antagonists & inhibitors* / Q000138:chemical synthesis; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D002273:Carcinogens; D005419:Flavonoids; D009573:Nitrites; D009602:Nitrosamines; D010636:Phenols; D010936:Plant Extracts; D002725:Chloroform; D004128:Dimethylnitrosamine