Lentibacillus halophilus sp. nov., from fish sauce in Thailand.
Fifteen strains of extremely halophilic bacteria were isolated from fish sauce (nam-pla) collected in Thailand at various stages of the fish-fermentation process. The isolates were strictly aerobic, spore-forming, Gram-positive rods. They grew optimally in the presence of 20-26 % NaCl. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C(15 : 0) and anteiso-C(17 : 0). Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The DNA G+C content was 42.1-43.1 mol%. On the basis of the 16S rRNA gene sequence, a representative strain, PS11-2(T), was found to be closely related to Lentibacillus juripiscarius JCM 12147(T) (97.3 % similarity). The 15 strains were included in the same species on the basis that the levels of DNA-DNA relatedness with strain PS11-2(T) were greater than 70 %. They could be distinguished from L. juripiscarius and other Lentibacillus species on the basis of several phenotypic characteristics and low levels of DNA-DNA relatedness (</=19.4 %). Therefore, the strains represent a novel species of the genus Lentibacillus, for which the name Lentibacillus halophilus sp. nov. is proposed. The type strain is PS11-2(T) (=JCM 12149(T)=TISTR 1549(T)=PCU 240(T)).
Tanasupawat
Somboon
S
Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Pathumwan, Bangkok 10330, Thailand.
Pakdeeto
Amnat
A
Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Pathumwan, Bangkok 10330, Thailand.
Namwong
Sirilak
S
Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Pathumwan, Bangkok 10330, Thailand.
Thawai
Chitti
C
Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Pathumwan, Bangkok 10330, Thailand.
Kudo
Takuji
T
Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Itoh
Takashi
T
Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
D000818:Animals; D001406:Bacillaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D003470:Culture Media; D003487:Cyanoacrylates / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D005396:Fish Products / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride; D013045:Species Specificity; D013785:Thailand; D024482:Vitamin K 2 / Q000031:analogs & derivatives
D003470:Culture Media; D003487:Cyanoacrylates; D004269:DNA, Bacterial; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C007166:cyacrin; D012965:Sodium Chloride; D003960:Diaminopimelic Acid; C062629:menaquinone 7