Changes in vibrational modes of water and bioprotectants in solution.
Inelastic neutron scattering (INS) measurements have been performed on trehalose and sucrose/H(2)O mixtures at very low temperature as a function of concentration by using the TOSCA spectrometer at the ISIS Facility (DRAL, UK). The aim of this work is to investigate by INS the vibrational behaviour of water in presence of trehalose and sucrose in order to characterize the changes induced by these disaccharides on the H(2)O hydrogen-bonded network. In particular, we obtained information about the effects of the two disaccharides in the translational, librational and bending spectral regions of ice. The disaccharide bioprotective effectiveness can be linked by the high destructuring effect emphasised by the analysis of the librational modes region. On the other hand, the analysis of the vibrational region corresponding to the ice bending modes show a high "crystallinity" degree which can justify the cryptobiotic action of disaccharides.
Magazù
S
S
Dipartimento di Fisica, Università di Messina, P.O. Box 55, S.ta Sperone C.da Papardo, 98166 Messina, Italy. smagazu@unime.it
Migliardo
F
F
Ramirez-Cuesta
A J
AJ
D003451:Cryoprotective Agents / Q000737:chemistry*; D006860:Hydrogen Bonding; D033363:Neutron Diffraction; D013395:Sucrose / Q000737:chemistry*; D014199:Trehalose / Q000737:chemistry*; D014732:Vibration; D014867:Water / Q000737:chemistry*
D003451:Cryoprotective Agents; D014867:Water; D013395:Sucrose; D014199:Trehalose