title: Effect of intramammary infusion of bacterial lipopolysaccharide on experimentally induced Staphylococcus aureus intramammary infection.
abstract: Mastitis due to Staphylococcus aureus is a significant problem in the dairy industry and is refractory to antibiotic treatment and/or vaccine prevention. Relative to other mastitis-causing pathogens, S. aureus elicits a diminutive host inflammatory response during intramammary infection. To determine whether induction of a heightened inflammatory response could influence outcome of infection, the highly pro-inflammatory molecule bacterial lipopolysaccharide (LPS) was infused into udder quarters experimentally infected with S. aureus. Relative to S. aureus-infected udder quarters receiving saline, quarters infused with LPS demonstrated a heightened inflammatory response as demonstrated by the induction of TNF-alpha and higher milk somatic cell counts and albumin levels. Although there was no overall effect on bacterial clearance, a trend toward reduced bacterial numbers during the immediate pro-inflammatory response following LPS infusion was observed suggesting that this novel approach to treating S. aureus intramammary infection may warrant further investigation.
authors.affiliation: Bovine Functional Genomics Laboratory, USDA-Agricultural Research Service, BARC-East, Building 1040, Room #2, Beltsville, MD 20705, United States.
authors.forename: Adam C W
authors.initials: AC
authors.lastname: Kauf
authors.forename: Bryan T
authors.initials: BT
authors.lastname: Vinyard
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Bannerman
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D007249:Inflammation / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008321:Mammary Glands, Animal / Q000187:drug effects*; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides