mig-5/Dsh controls cell fate determination and cell migration in C. elegans.
Cell fate determination and cell migration are two essential events in the development of an organism. We identify mig-5, a Dishevelled family member, as a gene that regulates several cell fate decisions and cell migrations that are important during C. elegans embryonic and larval development. In offspring from mig-5 mutants, cell migrations are defective during hypodermal morphogenesis, QL neuroblast migration, and the gonad arm migration led by the distal tip cells (DTCs). In addition to abnormal migration, DTC fate is affected, resulting in either an absent or an extra DTC. The cell fates of the anchor cell in hermaphrodites and the linker cells in the male gonad are also defective, often resulting in the cells adopting the fates of their sister lineage. Moreover, 2 degrees vulval precursor cells occasionally adopt the 3 degrees vulval cell fate, resulting in a deformed vulva, and the P12 hypodermal precursor often differentiates into a second P11 cell. These defects demonstrate that MIG-5 is essential in determining proper cell fate and cell migration throughout C. elegans development.
Walston
Timothy
T
Laboratory of Genetics, University of Wisconsin-Madison, 1117 W. Johnson St., Madison, WI 53706, USA.
Guo
Chaobo
C
Proenca
Rui
R
Wu
Mingfu
M
Herman
Michael
M
Hardin
Jeff
J
Hedgecock
Edward
E
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000502:physiology*; D000818:Animals; D019521:Body Patterning; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000502:physiology; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation; D002465:Cell Movement; D000072261:Dishevelled Proteins; D004625:Embryo, Nonmammalian; D005260:Female; D006066:Gonads / Q000166:cytology; D007814:Larva / Q000378:metabolism; D008297:Male; D009024:Morphogenesis; D010750:Phosphoproteins / Q000235:genetics / Q000502:physiology*; D020671:Posterior Horn Cells / Q000196:embryology / Q000502:physiology; D015398:Signal Transduction; D008941:Spindle Apparatus; D040521:Subcutaneous Tissue / Q000196:embryology; D014844:Vulva / Q000166:cytology / Q000196:embryology / Q000502:physiology
D048868:Adaptor Proteins, Signal Transducing; D029742:Caenorhabditis elegans Proteins; D000072261:Dishevelled Proteins; C515316:Mig-5 protein, C elegans; D010750:Phosphoproteins