title: [Detection of congenital toxoplasmosis in utero with a polymerase chain reaction on amniotic fluid].
abstract: To establish a prenatal diagnosis of congenital toxoplasmosis the PCR test was done on amniotic fluids from 47 women suspected of primary Toxoplasma infection during pregnancy. Fragments of Toxoplasma B1 gene were found in 5 examined samples. Positive tests were confirmed by mouse inoculation or by serologic testing of newborns. It was concluded that the PCR performed on amniotic fluid should be recommended for the prenatal diagnosis of congenital toxoplasmosis, however, negative results of the test can not rule out congenital infection.
authors.affiliation: Zakład Parazytologii Lekarskiej, PZH, ul. Chocimska 24, 00-791 Warszawa.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Gołab
authors.forename: Dorota
authors.initials: D
authors.lastname: Nowakowska
authors.forename: Maria
authors.initials: M
authors.lastname: Waloch
authors.forename: Tadeusz H
authors.initials: TH
authors.lastname: Dzbeński
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szaflik
authors.forename: Jan
authors.initials: J
authors.lastname: Wilczyński
mesh_terms: D000653:Amniotic Fluid / Q000469:parasitology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D016054:DNA, Protozoan / Q000302:isolation & purification; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis* / Q000469:parasitology; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis / Q000469:parasitology; D011296:Prenatal Diagnosis; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis / Q000469:parasitology; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan