title: [The comparison of properties of alpha-amylase from the third and fourth stage of Anisakis simplex larvae].
abstract: alpha-Amylase is present in the third (L3) and in the fourth-stage (L4) of larvae from Anisakis simplex. The enzymes from both sources differ in some of their properties. alpha-Amylase from L3 showed a maximum at pH 7.8, enzyme from L4 stage at pH 6.5. The alpha-amylase from L3 was mainly lysosomal enzyme. The enzyme from L4 was located in the microsomal fraction. The L3 alpha-amylase showed the inhibition by EDTA and by -SH reagent iodoacetic acid. These agents did not change the activity of L4 enzyme. Both izoenzymes were unaffected by calcium and magnesium ions. Generally the alpha-amylase from L4 stage had higher activity (3.71 u/mg) than L3 one (2.29 u/mg).
authors.affiliation: Zakład Biochemii, Wydział Biologii, UWM, 10-561 Olsztyn, ul. Zołnierska 14.
authors.forename: E
authors.initials: E
authors.lastname: Lopieńska
authors.forename: K
authors.initials: K
authors.lastname: Zołtowska
authors.forename: J
authors.initials: J
authors.lastname: Rokicki
mesh_terms: D000818:Animals; D017163:Anisakis / Q000201:enzymology* / Q000254:growth & development; D004795:Enzyme Stability; D005399:Fishes / Q000469:parasitology; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D007814:Larva / Q000201:enzymology* / Q000254:growth & development*; D008018:Life Cycle Stages / Q000502:physiology*; D008861:Microsomes / Q000737:chemistry; D014894:Weights and Measures; D000516:alpha-Amylases / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D000516:alpha-Amylases