title: Nitrile hydrolysis activity of Rhodococcus erythropolis NCIMB 11540 whole cells.
abstract: The nitrile hydrolyzing properties of the bacterium strain Rhodococcus erythropolis NCIMB 11540 have been investigated. Using whole cells of the microorganism, a wide variety of aromatic and aliphatic cyanide-containing substrates was successfully hydrolyzed to the corresponding amide or acid. In the case of dicyanides, selective monohydrolysis took place, which was further explored in the desymmetrization of malononitriles resulting in the corresponding cyano amides in enantiomeric excesses of up to 98%.
authors.affiliation: J.H. van 't Hoff Institute for Molecular Sciences, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Mandy K S
authors.initials: MK
authors.lastname: Vink
authors.forename: Roel
authors.initials: R
authors.lastname: Wijtmans
authors.forename: Christoph
authors.initials: C
authors.lastname: Reisinger
authors.forename: Rutger J F
authors.initials: RJ
authors.lastname: van den Berg
authors.forename: Christien A
authors.initials: CA
authors.lastname: Schortinghuis
authors.forename: Helmut
authors.initials: H
authors.lastname: Schwab
authors.forename: Hans E
authors.initials: HE
authors.lastname: Schoemaker
authors.forename: Floris P J T
authors.initials: FP
authors.lastname: Rutjes
mesh_terms: D006868:Hydrolysis; D009570:Nitriles / Q000378:metabolism*; D012240:Rhodococcus / Q000145:classification* / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D009570:Nitriles