Phosphodiester modification by zinc metalated adenine polymer with carboxyl pendants.
This report describes a novel carboxyl pendant containing adenylated polymeric template, its metalation with Zn (II), and manifestation of catalytic activity for the hydrolysis of model phosphodiester, bis(p-nitrophenyl) phosphate (bNPP), and plasmid cleavage. Observation of a bell-shaped pH-K(obs) profile suggested influence of pH variation over hydrolysis rate. This metalated polymer also afforded facile relaxation of pBR322 supercoiled DNA, with an interesting reusability feature intricately associated with heterogeneous catalysis.
Gupta
Yogita
Y
Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur 208016, India.
Mathur
G N
GN
Parvez
Masood
M
Verma
Sandeep
S
D000225:Adenine / Q000737:chemistry*; D002384:Catalysis; D004278:DNA, Superhelical / Q000737:chemistry; D019281:Dimerization; D004952:Esters / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008958:Models, Molecular; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D013997:Time Factors; D015032:Zinc / Q000737:chemistry*
D004278:DNA, Superhelical; D004952:Esters; D011108:Polymers; D015032:Zinc; D000225:Adenine