title: Leukocytes-depleting filters preferentially remove activated leukocytes and reduce the expression of surface adhesion molecules during the simulated extracorporeal circulation of human blood.
abstract: The effect of leukocyte-depleting filters on the total and activated leukocyte counts and the expression of surface adhesion molecules CD11b, CD18, and CD62L during the in vitro extracorporeal circulation of human blood was studied. A 200 ml blood sample was taken from 10 patients undergoing CABG surgery. The blood was circulated for 60 minutes within an experimental extracorporeal circuit. A leukocyte-depleting filter was attached in five circuits (filtered group). In five other circuits, no filter was used (controls). Total leukocyte counts were determined manually. Activated leukocytes were identified using nitroblue tetrazolium staining. The expression of CD11b, CD18, and CD62L was measured with flow cytometry. At 60 minutes, total leukocyte counts were reduced by 49% from the baseline values in the filtered group and 10% in the control group (p < 0.0001). Activated leukocyte counts decreased by 86% in the filtered group and increased by 116% in the control group (p < 0.0001). In the filtered group, the expression of CD11b, CD18, and CD612L decreased by 60%, 21%, and 79%, respectively, and in the control group it increased by 24%, 6%, and 28% (p < 0.0001). Leukocyte-depleting filters preferentially remove activated leukocytes and reduce the expression of CD11b, CD18, and CD62L during the in vitro extracorporeal circulation of human blood.
authors.affiliation: Departments of Cardiac Surgery, The General Hospital, Southampton, UK.
authors.forename: Christos
authors.initials: C
authors.lastname: Alexiou
authors.forename: Stuart
authors.initials: S
authors.lastname: Sheppard
authors.forename: Augustine
authors.initials: A
authors.lastname: Tang
authors.forename: Arvind
authors.initials: A
authors.lastname: Rengarajan
authors.forename: David
authors.initials: D
authors.lastname: Smith
authors.forename: Marcus
authors.initials: M
authors.lastname: Haw
authors.forename: Roz
authors.initials: R
authors.lastname: Gibbs
mesh_terms: D000368:Aged; D039481:CD11b Antigen / Q000097:blood / Q000378:metabolism; D018821:CD18 Antigens / Q000097:blood / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003198:Computer Simulation; D001026:Coronary Artery Bypass; D005112:Extracorporeal Circulation; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D019041:L-Selectin / Q000097:blood / Q000378:metabolism; D007958:Leukocyte Count; D047589:Leukocyte Reduction Procedures / Q000295:instrumentation* / Q000379:methods; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D013997:Time Factors
chemical_list: D039481:CD11b Antigen; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D019041:L-Selectin