Estrogen and ghrelin decrease cytoplasmic expression of p27kip1, a cellular marker of ageing, in the striated anal sphincter and levator muscle of ovariectomized rats.
A study was carried out to investigate the effect of estrogen and/or ghrelin on the cellular marker of ageing, p27kip1, in pelvic floor muscles of ovariectomized rats. Virgin Wistar rats (13 months old) underwent ovariectomy followed (1 month) by 42 daily intraperitoneal 17-beta estradiol (10 microg/kg), ghrelin (2 microg/kg), both hormones, or placebo vehicle (n=6x4 groups). Six more age-matched animals underwent sham surgery without ovariectomy. Cytoplasmic expression of p27kip1 in the striated urethral and anal sphincters and levator muscle was measured by Western blot analysis in all animals (n=30). p27kip1 signal intensity significantly increased postovariectomy in all muscles compared to sham animals. In the anal sphincter and levator, signal intensity decreased to sham levels with ghrelin or estrogen and decreased further after estrogen or ghrelin and estrogen/ghrelin administration. Urethral sphincter signal intensity decreased without reaching sham levels after drug administration. Estrogen and/or ghrelin replacement reverses the ovariectomy-induced exacerbation of biochemical cellular ageing in the anal sphincter and levator muscle of middle-aged rats.
Rizk
Diaa E E
DE
Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates. rizk.diaa@uaeu.ac.ae
Al-Marzouqi
Ahmed H
AH
Hassan
Hazem A
HA
Al-Kedrah
Soha S
SS
Fahim
Mohamed A
MA
D000375:Aging / Q000502:physiology; D001003:Anal Canal / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27; D003593:Cytoplasm / Q000187:drug effects* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D054439:Ghrelin; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D010052:Ovariectomy; D017773:Pelvic Floor / Q000502:physiology*; D036361:Peptide Hormones / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; C436291:CDKN1B protein, human; D054439:Ghrelin; D047908:Intracellular Signaling Peptides and Proteins; D036361:Peptide Hormones; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004958:Estradiol