title: Nanoscale resolution in GFP-based microscopy.
abstract: We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Katrin I
authors.initials: KI
authors.lastname: Willig
authors.forename: Robert R
authors.initials: RR
authors.lastname: Kellner
authors.forename: Rebecca
authors.initials: R
authors.lastname: Medda
authors.forename: Birka
authors.initials: B
authors.lastname: Hein
authors.forename: Stefan
authors.initials: S
authors.lastname: Jakobs
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D000818:Animals; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D021621:Imaging, Three-Dimensional; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000451:nursing*; D008870:Microtubules / Q000648:ultrastructure*; D036103:Nanotechnology; D025682:Nanovirus; D021381:Protein Transport; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins