Human soluble E-selectin immunoadhesin inhibits leukemic monocyte adhesion to endothelial cells.
Immunoadhesins are immunoglobulin (Ig)-like chimeric proteins comprised of target-binding regions fused to the Fc-hinge region of Ig, and are designed to have a long half-life and antibody-like properties. In an effort to find a good candidate for therapeutic use for inflammatory responses, we constructed a soluble human E-selectin immunoadhesin containing the extracellular region of human E-selectin fused to the Fc-hinge region of human IgG, and determined its effects on leukocyte adhesion and rolling in vitro. Our results revealed that the adhesion of leukocytes to endothelial cells was efficiently inhibited in the presence of 50 nM E-selectin immunoadhesin. In addition, the E-selectin immunoadhesin significantly inhibited leukocyte rolling on endothelial cells in perfusion experiments performed at 1.0 dyne/cm(2) wall shear stress. These findings indicate that our E-selectin immunoadhesin decreases leukocyte attachment and rolling in vitro, suggesting that this immunoadhesin may be a promising candidate for therapeutic anti-inflammatory use.
Sung
Bong Hwan
BH
Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
Kim
Sung Hyun
SH
Yeo
Myeong Gu
MG
Kim
Jin-Kyoo
JK
Song
Woo Keun
WK
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D019040:E-Selectin / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D036904:Leukocyte Rolling; D009000:Monocytes / Q000378:metabolism / Q000473:pathology*; D012995:Solubility
D019040:E-Selectin