title: Synthesis and in vitro sorption properties of PAA-grafted cellulose beads for selective binding of LDL.
abstract: Poly(acrylic acid) (PAA)-grafted cellulose copolymer beads were synthesized and tested in vitro as an adsorbent for selective removal of low-density lipoprotein (LDL) from human plasma. The copolymers were prepared by graft copolymerization of acrylic acid (AA) onto porous cellulose beads using cerium ammonium nitrate (CAN) as an initiator. The effect of initiator concentration, monomer amount and reaction time on the grafting was examined, and it revealed that the extent of grafting could be controlled by setting the appropriate reaction conditions. In vitro batch-wise adsorption tests were conducted to evaluate the lipoprotein sorption properties of the resulted copolymer beads, and the effect of grafting conditions on the adsorption performance was investigated. It was shown that the binding capacities of the best adsorbent derived from the appropriate reaction conditions could reach 4.96 mg/g total cholesterol (TC) and 4.46 mg/g LDL cholesterol (LDL-C) from human plasma, respectively, without significantly affecting the contents of beneficial constitutes such as high-density lipoprotein (HDL) and total proteins (TP). The influences of plasma amount and adsorption period on the adsorption properties were also determined and analyzed. It appears that this kind of copolymer is worthy of being developed as an alternative LDL adsorbent.
authors.affiliation: The Key Laboratory of Functional Polymer Materials, Institute of Polymer Chemistry, Nankai University, Tianjin, PR China.
authors.forename: Haofeng
authors.initials: H
authors.lastname: Yu
authors.forename: Guoqi
authors.initials: G
authors.lastname: Fu
authors.forename: Jichao
authors.initials: J
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Binglin
authors.initials: B
authors.lastname: He
mesh_terms: D000179:Acrylates / Q000737:chemistry* / Q000493:pharmacokinetics; D000327:Adsorption; D001781:Blood Component Removal / Q000379:methods*; D002482:Cellulose / Q000737:chemistry; D008078:Cholesterol, LDL / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D006937:Hypercholesterolemia / Q000628:therapy*; D066298:In Vitro Techniques; D007700:Kinetics; D008863:Microspheres
chemical_list: D000179:Acrylates; D008078:Cholesterol, LDL; D002482:Cellulose; C036658:acrylic acid