title: A 1H STD NMR spectroscopic investigation of sialylnucleoside mimetics as probes of CMP-Kdn synthetase.
abstract: CMP-Kdn synthetase catalyses the reaction of sialic acids (Sia) and CTP to the corresponding activated sugar nucleotide CMP-Sia and pyrophosphate PP( i ). Saturation Transfer Difference (STD) NMR spectroscopy has been employed to investigate the sub-structural requirements of the enzyme's binding domain. Sialylnucleoside mimetics, where the sialic acid moiety has been replaced by a carboxyl group and a hydrophobic moiety, have been used in NMR experiments, to probe the tolerance of the CMP-Kdn synthetase to such replacements. From our data it would appear that unlike another sialylnucleotide-recognising protein, the CMP-Neu5Ac transport protein, either a phosphate group or other functional groups on the sialic acid framework may play important roles in recognition by the synthetase.
authors.affiliation: Institute for Glycomics, Griffith University (Gold Coast Campus), PMB 50 Gold Coast Mail Centre, Queensland 9726, Australia.
authors.forename: Thomas
authors.initials: T
authors.lastname: Haselhorst
authors.forename: Melanie
authors.initials: M
authors.lastname: Oschlies
authors.forename: Tareq
authors.initials: T
authors.lastname: Abu-Izneid
authors.forename: Milton J
authors.initials: MJ
authors.lastname: Kiefel
authors.forename: Joe
authors.initials: J
authors.lastname: Tiralongo
authors.forename: Anja K
authors.initials: AK
authors.lastname: Münster-Kühnel
authors.forename: Rita
authors.initials: R
authors.lastname: Gerardy-Schahn
authors.forename: Mark
authors.initials: M
authors.lastname: von Itzstein
mesh_terms: D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D018716:Molecular Mimicry; D015335:Molecular Probes / Q000737:chemistry*; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry* / Q000378:metabolism; D009705:Nucleosides / Q000737:chemistry* / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D009705:Nucleosides; C079494:CTP-CMP-3-deoxynonulosonate cytidyltransferase; D009713:Nucleotidyltransferases; D019158:N-Acetylneuraminic Acid