Comparison of the effect of Ascaris trypsin inhibitor in various periods of mouse pregnancy.
Trypsin inhibitor isolated from Ascaris suum and injected into pregnant BALB/c mice (five times, in doses: 300 or 400 mg/kg/day) in various periods of pregnancy (early and late organogenesis) disturbed the development of fetuses. The nature and intensity of prenatal disturbances are determined by the inhibitor dose and time of injection. It has been found that administration of the inhibitor from 5-th until 9-th day of gestation did not delay or prevent implantation, but caused a high rate of intrauterine deaths and also specific congenital malformations (exnencephaly and hydrocephalus). Additionally, other types of defects were noted in fetuses after injection of the inhibitor between 8-th and 12-th day of pregnancy (cleft palate, fusion of ribs). Independent of the time of injection during gestation the inhibitor exhibited embriotoxic effects (e.g. decreased the number of live fetuses per litter and mean fetal weight).
Błaszkowska
J
J
Chair of Medical Biology and Parasitology, Medical University of Lódź, 90-436 Lódź, al. Kościuszki 85.
D000014:Abnormalities, Drug-Induced / Q000469:parasitology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005313:Fetal Death / Q000139:chemically induced; D020567:Fetal Weight / Q000187:drug effects; D005865:Gestational Age; D015801:Helminth Proteins / Q000633:toxicity*; D008098:Litter Size / Q000187:drug effects; D008431:Maternal-Fetal Exchange / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D038081:Organogenesis / Q000187:drug effects; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000139:chemically induced*; D011297:Prenatal Exposure Delayed Effects; D013723:Teratogens / Q000633:toxicity*
D015801:Helminth Proteins; D013723:Teratogens; C089852:trypsin inhibitor, Ascaris