title: A tuber lectin from Arisaema jacquemontii Blume with anti-insect and anti-proliferative properties.
abstract: A tuber lectin from Arisaema jacquemontii Blume belonging to family Araceae was purified by employing a single step affinity chromatography using column of asialofetuin-linked amino activated silica beads and the bound lectin was eluted with 100 mM glycine-HCl buffer pH 2.5. The purified A. jacquemontii lectin (AJL) showed a single protein band with an apparent molecular mass of 13.4 kDa when submitted to SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. The native molecular mass of AJL determined by gel filtration on a Biogel P-200 column was 52 kDa and its carbohydrate content was estimated to be 3.40%. Thus AJL is a tetrameric glycoprotein. The purified lectin agglutinated erythrocytes from rabbit but not from human. Its activity was not inhibited by any of the mono- and disaccharides tested except N-acetyl-D-lactosamine having minimal inhibitory sugar concentration (MIC) 25 mM. Among the glycoproteins tested only asialofetuin was found to be inhibitory (MIC125 microg/mL). A single band was obtained in native PAGE at pH 4.5 while PAGE at pH 8.3 showed two bands. Isoelectric focusing of AJL gave multiple bands in the pI range of 4.6-5.5. When incorporated in artificial diet AJL significantly affected the development of Bactrocera cucurbitae (Coquillett) larvae indicating the possibility of using this lectin in a biotechnological strategy for insect management of cucurbits. Larvae fed on artificial diet containing sublethal dose of AJL showed a significant decrease in acid phosphatase and alkaline phosphatase activity while esterase activity markedly increased as compared to larvae fed on diet without lectin. Out of various human cancer cell lines employed in sulphorhodamine B (SRB) assay, this lectin was found to have appreciable inhibitory effect on the in vitro proliferation of HCT-15, HOP-62, SW-620, HT-29, IMR-32, SKOV-3, Colo-205, PC-3, HEP-2 and A-549 cancer cell lines by 82, 77, 73, 70, 41, 41, 37, 29, 21 and 21% respectively.
authors.affiliation: Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar 143 005, India.
authors.forename: Manpreet
authors.initials: M
authors.lastname: Kaur
authors.forename: Kuljinder
authors.initials: K
authors.lastname: Singh
authors.forename: Pushpinder Jai
authors.initials: PJ
authors.lastname: Rup
authors.forename: Sukhdev Singh
authors.initials: SS
authors.lastname: Kamboj
authors.forename: Ajit Kumar
authors.initials: AK
authors.lastname: Saxena
authors.forename: Madhunika
authors.initials: M
authors.lastname: Sharma
authors.forename: Madhulika
authors.initials: M
authors.lastname: Bhagat
authors.forename: Sarvesh Kumar
authors.initials: SK
authors.lastname: Sood
authors.forename: Jatinder
authors.initials: J
authors.lastname: Singh
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D031057:Arisaema / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000302:isolation & purification / Q000494:pharmacology*; D037121:Plant Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D013696:Temperature; D033621:Tephritidae / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D007306:Insecticides; D037121:Plant Lectins