Nanostructured polymer matrix for oligonucleotide separation.
A nanostructured copolymer matrix has successfully separated oligonucleotides with high resolution by CE using a very short separation channel which simulates the real microchip condition for the first time. The triblock copolymer, E(45)B(14)E(45) (B20-5000) with E, B, and subscript denoting oxyethylene, oxybutylene, and segment length, respectively, has a unique temperature-dependent viscosity-adjustable property and a dynamic coating ability in 1xTBE buffer (89 mM Tris, 89 mM boric acid, 2 mM EDTA in Milli-Q water). The B-block is hydrophilic at low temperatures, e.g., 4 degrees C, and the polymer solution has a very low viscosity of about 100 cP in a 32.5% w/v solution. At room temperatures, the B-block becomes hydrophobic due to a breakdown of hydrogen bonds between B-blocks and water, and the polymer matrix forms a body-centered cubic structure at high concentrations. Oligonucleotide sizing markers ranging from 8 to 32 base could be successfully separated with one-base resolution in a 1.5 cm long separation channel by E(45)B(14)E(45) in its gel-like state.
Zhang
Jun
J
Department of Chemistry, Stony Brook University, NY 11794-3400, USA.
Burger
Christian
C
Chu
Benjamin
B
D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D008027:Light; D036103:Nanotechnology / Q000379:methods; D009841:Oligonucleotides / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012542:Scattering, Radiation; D014965:X-Rays
D009841:Oligonucleotides; C514700:poly(ethylene oxide)-poly(butylene oxide)-poly(ethylene oxide); D011092:Polyethylene Glycols