Repetitive fluctuations in blood glucose enhance monocyte adhesion to the endothelium of rat thoracic aorta.
BACKGROUND
The aim of this study was to elucidate the effect of repetitive fluctuations in blood glucose concentrations on monocyte adhesion to the aortic endothelium.


RESULTS
Nonobese type 2 diabetes, Goto-Kakizaki (GK) rats were fed twice daily to induce repetitive postprandial glucose spikes. Then, we compared the number of monocytes adherent to the endothelium of thoracic aorta in these rats with that in rats fed ad libitum. To suppress the glucose spikes, rats were injected with an inhibitor of sodium-glucose transporter, phloridzin, just before each meal for 12 weeks. GK rats fed twice daily showed significantly lower HbA1c than GK rats fed ad libitum. However, the former group showed markedly higher number of monocytes adherent to the endothelium than the latter, together with increased arterial intimal thickening. Phloridzin significantly reduced the number of adherent monocytes in GK rats fed twice daily.


CONCLUSIONS
Our data demonstrated that repetitive postprandial fluctuation in glucose concentration evokes monocyte adhesion to endothelial cells that was worse than that induced by stable hyperglycemia in vivo. Suppression of such fluctuations efficiently suppressed monocyte adhesion to the aortic endothelium.
Azuma
Kosuke
K
Department of Medicine, Metabolism and Endocrinology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
Kawamori
Ryuzo
R
Toyofuku
Yukiko
Y
Kitahara
Yoshiro
Y
Sato
Fumihiko
F
Shimizu
Tomoaki
T
Miura
Kyoko
K
Mine
Tomoyuki
T
Tanaka
Yasushi
Y
Mitsumata
Masako
M
Watada
Hirotaka
H
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D001786:Blood Glucose / Q000378:metabolism; D002448:Cell Adhesion; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005508:Food Deprivation; D006943:Hyperglycemia / Q000097:blood / Q000150:complications / Q000503:physiopathology*; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D009000:Monocytes; D010455:Peptides / Q000378:metabolism; D019518:Postprandial Period; D051381:Rats; D011919:Rats, Inbred Strains; D012008:Recurrence; D017539:Tunica Intima / Q000473:pathology
D001786:Blood Glucose; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C076687:CS1 peptide