title: Studies on immunogenicity and protective efficacy of DnaJ of Salmonella Typhi against lethal infection by Salmonella Typhimurium in mice.
abstract: In the present study DnaJ (HSP40) of Salmonella enterica serovar Typhi has been evaluated for its immunogenicity and efficacy in protecting mice against lethal challenge by S. enterica serovar Typhimurium infection. DnaJ was amplified by PCR of the genomic DNA of S. Typhi and subsequently cloned in pQE-30 expression vector. The protein was induced by IPTG and purified using Ni-NTA chromatography under denaturing conditions. After refolding in vitro the immune response was evaluated by injecting 40 microg DnaJ protein/mouse i.p. on 0th, 7th and 28th day. The results showed a significant increase in antibody titre and lymphocyte proliferation in animals immunised with DnaJ as compared to control. Further there was an appreciable increase in IL-2, IL-4, IFN-gamma production in lymphocytes isolated from immunised mice as compared to control. In this limited study, immunisation of mice with DnaJ was found to provide 70% protection against lethal challenge by S. Typhimurium indicating the possible use of DnaJ as vaccine candidate against typhoid.
authors.affiliation: Department of Experimental Biology, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi 110054, India.
authors.forename: Sarada S K
authors.initials: SS
authors.lastname: Sagi
authors.forename: Piyush
authors.initials: P
authors.lastname: Paliwal
authors.forename: Anju
authors.initials: A
authors.lastname: Bansal
authors.forename: Chittaranjan
authors.initials: C
authors.lastname: Mishra
authors.forename: Nadeem
authors.initials: N
authors.lastname: Khan
authors.forename: Sai Ram
authors.initials: SR
authors.lastname: Mustoori
authors.forename: G
authors.initials: G
authors.lastname: Ilavazhagan
authors.forename: R C
authors.initials: RC
authors.lastname: Sawhney
authors.forename: P K
authors.initials: PK
authors.lastname: Banerjee
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D049109:Cell Proliferation; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007371:Interferon-gamma / Q000032:analysis / Q000378:metabolism; D007376:Interleukin-2 / Q000032:analysis / Q000378:metabolism; D015847:Interleukin-4 / Q000032:analysis / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012480:Salmonella Infections / Q000276:immunology* / Q000517:prevention & control*; D012485:Salmonella typhi / Q000276:immunology*; D012486:Salmonella typhimurium; D019444:Vaccines, DNA / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D007074:Immunoglobulin G; D007376:Interleukin-2; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D015847:Interleukin-4; D007371:Interferon-gamma