title: Gonadotrophin levels and morphological testicular features in rats after different doses of the phytoestrogen coumestrol.
abstract: BACKGROUND
  Phytoestrogens are a wide variety of chemical compounds, mainly isoflavonoids, from a vegetable source. Their name makes reference to their ability to induce estrogenic responses in mammals. Coumestrol is a phytoestrogen that can be found in high concentrations in the dietary elements of cattle. Some endocrine alterations have been reported for cows and ewes after ingestion of vegetables with high concentrations of coumestrol. However, these studies have been made mainly in females.
  
  
  OBJECTIVE
  To analyze some features of the masculine endocrine response in rats after several doses of the phytoestrogen coumestrol.
  
  
  METHODS
  Adult male rats were injected with several doses of coumestrol. Plasma gonadotrophins (LH/FSH) and testosterone levels were assessed. In addition, morphology of the testicles was analyzed.
  
  
  METHODS
  Experiments were done in the facilities of the neurosciences area at the University.
  
  
  RESULTS
  No significant changes were observed in gonadotrophin levels after the administration of coumestrol. Testosterone levels showed a significant decrease with the higher doses. Morphological analysis showed an inhibitory effect on spermatogenesis expressed mainly in the right testicle. Testicular volume decreased and the tubular area increased significantly after coumestrol treatment.
  
  
  CONCLUSIONS
  These results suggest that the endocrine effect of coumestrol is mainly expressed in peripheral targets in male rats. In addition, the possible mediation of estrogen beta receptors is discussed.
authors.affiliation: Department of Biology of Reproduction, Universidad Autónoma Metropolitana-Iztapalapa, CP 09340, México City, Mexico.
authors.forename: C Rosario
authors.initials: CR
authors.lastname: Tarragó-Castellanos
authors.forename: C Mario
authors.initials: CM
authors.lastname: García-Lorenzana
authors.forename: Vicente
authors.initials: V
authors.lastname: Diaz-Sánchez
authors.forename: Javier
authors.initials: J
authors.lastname: Velázquez-Moctezuma
mesh_terms: D000818:Animals; D003375:Coumestrol / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000097:blood; D006728:Hormones / Q000097:blood*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009929:Organ Size / Q000187:drug effects; D048789:Phytoestrogens / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D013739:Testosterone / Q000097:blood
chemical_list: D006728:Hormones; D048789:Phytoestrogens; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D003375:Coumestrol