Detection of Rev peptides with impedance-sensors--comparison of device-geometries.
Two different impedance-sensor geometries have been compared for the detection of Rev peptides with a molecular weight of 2.4 kDa. Planar, two-dimensional interdigitated capacitor (IDC) sensors with electrode separations of 1.1 microm as well as three-dimensional nanogap-sensors with an electrode separation of 75 nm have been used. Both sensors have been operated at a fixed frequency of 980 MHz. We discuss the specific interaction of the Rev peptide to an immobilized RNA anti-Rev aptamer (9.2 kDa) for peptide concentrations in the range of 100 nM-2 microM. For the IDC sensor, only peptide concentrations above 500 nM gave detectable signals. For the nanogap sensor, the binding process was clearly visible for all concentrations applied. The higher sensitivity of the nanogap compared to the IDC is ascribed to the improved surface-to-volume ratio.
Schlecht
U
U
Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53173 Bonn, Germany. schlecht@caesar.de
Malavé
A
A
Gronewold
T M A
TM
Tewes
M
M
Löhndorf
M
M
D015374:Biosensing Techniques; D017097:Electric Impedance; D015694:Gene Products, rev / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*
D015694:Gene Products, rev; D010455:Peptides