[Hemangiopoiet in modulates adhesive properties of endothelial cells].
OBJECTIVE
To explore the effect of hemangiopoietin (HAPO) on the adhesive properties of human umbilical vein endothelial cells (HUVEC).


METHODS
The adhesion of HUVEC and the expressions of CD54, CD102, CD106, CD31, CD62E, and CD62P were measured by adhesion assay, flow cytometry, and semi-quantitative RT-PCR.


RESULTS
HAPO enhanced the total adherence of HUVEC in a concentration-dependent manner. Flow cytometry analysis revealed that the treatment of HAPO resulted in a significantly increased expression of CD106 and CD62E on HUVEC in a time-dependent manner. When HUVEC were incubated with HAPO for 6 h, the percentage of CD106 + HUVEC and CD62E HUVEC increased about 2.10 folds and 5.84 folds, respectively, compared with control. The time-course of adhesive molecules mRNA expression indicated that the expression of CD106 and CD62E reached at the maximum 1.86 folds and 6.16 folds, respectively, compared with control.


CONCLUSIONS
HAPO may facilitate the homing of hematopoietic stem/progenitor cells.
Wang
Xiao-Jing
XJ
State Key Laboratory of Experimental Hematology, Tianjin Economic-Technological Development Area Life and Technology Research Center, Institute of Hematology, CAMS and PUMC, Tianjin 300020, China.
Liu
Yong-Jun
YJ
Lu
Shi-Hong
SH
Liang
Lin-Hui
LH
Wang
Han
H
Han
Zhong-Chao
ZC
D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D019040:E-Selectin / Q000096:biosynthesis / Q000235:genetics; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D011509:Proteoglycans / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D000099158:Proteoglycan 4
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1; D011509:Proteoglycans; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; C419197:PRG4 protein, human; D000099158:Proteoglycan 4