PPARalpha and PPARgamma regulation of liver and adipose proteins in obese and dyslipidemic rodents.
Zucker fatty rats and ob/ob mice are both frequently used hyperlipidemic and insulin-resistant spontaneous genetic models of obesity. We used them to study the effect of PPAR agonists on the protein-expression level in liver and white adipose tissue. PPARalpha-agonist treatments of the rats resulted in that 27% of the quantified hepatic proteins were altered; implicating pronounced peroxisome proliferation and increase in capacity for beta-oxidation of fatty acids although no correction of plasma triglycerides were obtained. On treatment with PPARgamma agonists, adipose proteins were regulated to a much larger extent in the rats compared to mice, 18% and 2%, respectively.
Lanne
Boel
B
AstraZeneca R&D Mölndal, S-431 83 Mölndal, Sweden. boel.s.lanne@astrazeneca.com
Dahllöf
Björn
B
Lindahl
Charlotta
C
Ebefors
Kerstin
K
Kanmert
Ida
I
von Bahr
Helena
H
Miliotis
Tasso
T
Nyström
Ann-Christin
AC
Arnerup
Gunnel
G
Paulsons
Ivar
I
Kerb
Santy
S
Oakes
Nick
N
D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D050171:Dyslipidemias / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D008099:Liver / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008820:Mice, Obese; D047493:PPAR alpha / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D047495:PPAR gamma / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D020025:Peroxisome Proliferators / Q000494:pharmacology; D020543:Proteome / Q000032:analysis; D011743:Pyrimidines / Q000494:pharmacology; D051381:Rats; D011924:Rats, Zucker; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology
D007004:Hypoglycemic Agents; D047493:PPAR alpha; D047495:PPAR gamma; D020025:Peroxisome Proliferators; D020543:Proteome; D011743:Pyrimidines; D045162:Thiazolidinediones; D000077154:Rosiglitazone; C006253:pirinixic acid