Studies on the hemolytic activity of tentacle extracts of jellyfish Rhopilema esculentum Kishinouye: application of orthogonal test.
The present work is first reporting the hemolytic activity of venom from jellyfish Rhopilema esculentum Kishinouye extracted by different phosphate buffer solutions and incubated at different temperature according to the orthogonal test L6(1) x 3(6). Of the seven controllable independent variables, incubated temperature and phenylmethylsulfonyl fluoride (PMSF) had strongest effect on the hemolytic activity.
Yu
Huahua
H
Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
Xing
Ronge
R
Liu
Song
S
Li
Cuiping
C
Guo
Zhanyong
Z
Li
Pengcheng
P
D000818:Animals; D002645:Chickens; D003064:Cnidarian Venoms / Q000737:chemistry*; D006461:Hemolysis; D006358:Hot Temperature; D010664:Phenylmethylsulfonyl Fluoride / Q000737:chemistry; D011817:Rabbits; D037401:Scyphozoa / Q000737:chemistry*
D003064:Cnidarian Venoms; D010664:Phenylmethylsulfonyl Fluoride