Candida albicans Berthout, 1923: hydrolases activity and own method of digestive tract strains biotyping.
112 strains of Candida albicans were isolated from oral cavity and ontocenoses of the upper digestive tract (endoscopy) of children (age: 5-17) with gastrointestinal disorders. Axenic strains were differentiated with API 20C AUX and API ZYM tests (bioMérieux). Then enzymograms and biotypes were determined for all the strains based on the activity of 19 hydrolases. The highest activity was noted for: e2 - phosphatase alcaline, e6 - leucine arylamidase, e11 - phosphatase acid, e5 - lipase (c14); e7 - valine arylamidase, e12 - naphtol-AS-BI-phosphohydrolase, e16 - alpha-glucosidase and e18 - N-acetyl-beta-glucosamidase, and the latter four were used for biotyping procedures. Our own system was based on the mathematical binominal distribution formula (1 : 4 : 6 : 4 : 1): all "+"; one "-", three "+"; two "-", two "+"; three "-", one "+"; all "-". We have found the following biotypes: A (16.1 +/- 3.5%), B1 (2.7 +/- 2.53%), B3 (8.0 +/- 2.5%), B4 (22.3 +/- 3.9%), C2 (1.8 +/- 1.3%), C3 (7.1 +/- 2.4%), C6 (30.4 +/- 4.3%), D, (11.6 +/- 3.0%).
Kurnatowski
Marek
M
Clinic of Children's Gastroenterology and Allergology, Medical University, Sporna 36/50, 91-738 Lódź.
Wasowska-Królikowska
Krystyna
K
Kurnatowska
Alicja
A
D000293:Adolescent; D000946:Antigens, Fungal / Q000032:analysis / Q000145:classification; D002176:Candida albicans / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000382:microbiology; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D007410:Intestinal Diseases / Q000382:microbiology*; D008297:Male; D009055:Mouth / Q000382:microbiology; D016533:Mycological Typing Techniques; D013045:Species Specificity; D013270:Stomach / Q000382:microbiology
D000946:Antigens, Fungal; D006867:Hydrolases