title: VIP decreases TLR4 expression induced by LPS and TNF-alpha treatment in human synovial fibroblasts.
abstract: It has been demonstrated that VIP produces beneficial effects both in a murine model of rheumatoid arthritis and in human rheumatoid synovial fibroblasts through the modulation of proinflammatory mediators. Toll-like receptors (TLRs) play a key role in the immediate recognition of microbial surface components by immune cells prior to the development of adaptative microbe-specific immune responses. In this study, we demonstrate that VIP decreases lipopolysaccharide (LPS) and TNF-alpha-induced expression of TLR4 and its correlation with the production of CCL2 and CXCL8 chemokines in human synovial fibroblasts from patients with rheumatoid arthritis and osteoarthritis. Our results add a new step for the use of VIP, as a promising candidate, for the treatment of rheumatoid arthritis.
authors.affiliation: Departamento de Biología Celular, Facultad de Biología, UCM, 28040 Madrid, Spain.
authors.forename: Y
authors.initials: Y
authors.lastname: Juarranz
authors.forename: I
authors.initials: I
authors.lastname: Gutiérrez-Cañas
authors.forename: A
authors.initials: A
authors.lastname: Arranz
authors.forename: C
authors.initials: C
authors.lastname: Martínez
authors.forename: C
authors.initials: C
authors.lastname: Abad
authors.forename: J
authors.initials: J
authors.lastname: Leceta
authors.forename: J L
authors.initials: JL
authors.lastname: Pablos
authors.forename: R P
authors.initials: RP
authors.lastname: Gomariz
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D013583:Synovial Membrane / Q000187:drug effects* / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
chemical_list: D008070:Lipopolysaccharides; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D014660:Vasoactive Intestinal Peptide