title: Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicus.
abstract: A restriction endonuclease activity from Sulfolobus islandicus REN2H1 was purified by phosphocellulose and cation exchange chromatography. The enzyme cuts DNA at the recognition site GCwGC as could be shown by restriction analysis of plasmids and short synthetic duplex DNA. The cleavage occurs after the first guanosine base and is inhibited by 5-methyl-cytosine methylation. The restriction activity is salt-sensitive and has an optimal activity around 70 degrees C.
authors.affiliation: Institute of Biochemistry, University of Bayreuth, Universitätstrasse 30, 95447 Bayreuth, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Söllner
authors.forename: Silvia
authors.initials: S
authors.lastname: Berkner
authors.forename: Georg
authors.initials: G
authors.lastname: Lipps
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002411:Cation Exchange Resins; D002482:Cellulose / Q000031:analogs & derivatives; D002852:Chromatography, Ion Exchange / Q000379:methods; D003596:Cytosine / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D019175:DNA Methylation; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011489:Protein Denaturation; D012965:Sodium Chloride / Q000737:chemistry; D016944:Sulfolobus / Q000201:enzymology*; D013696:Temperature
chemical_list: D019843:Archaeal Proteins; D002411:Cation Exchange Resins; D012965:Sodium Chloride; D003596:Cytosine; D002482:Cellulose; D004247:DNA; C008881:phosphocellulose; D015252:Deoxyribonucleases, Type II Site-Specific