Gillisia myxillae sp. nov., a novel member of the family Flavobacteriaceae, isolated from the marine sponge Myxilla incrustans.
A yellow-pigmented, Gram-negative, rod-shaped, strictly aerobic bacterium (strain UST050418-085(T)) was isolated from the surface of a marine sponge, Myxilla incrustans, at Friday Harbor, WA, USA. The DNA G+C content of this strain was 34.6 mol%. The predominant fatty acids were i15 : 0, a15 : 0, i15 : 1, i16 : 0, i17 : 0 3-OH, 17 : 0 2-OH and summed feature 3, comprising i15 : 0 2-OH and/or 16 : 1omega7c (altogether representing 69.0 % of the total fatty acids). MK-6 was the only respiratory quinone detected. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of UST050418-085(T) were members of the genus Gillisia, with sequence similarities of 93.2-96.6 %. Strain UST050418-085(T) differed from its closest relatives by 11 to 18 phenotypic traits. Molecular evidence and phenotypic characteristics suggest that strain UST050418-085(T) represents a novel species within the genus Gillisia. The name Gillisia myxillae sp. nov. is proposed, with UST050418-085(T) (=JCM 13564(T)=NRRL B-41416(T)) [corrected] as the type strain.
Lee
On On
OO
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, People's Republic of China.
Lau
Stanley C K
SCK
Division of Environmental Science and Engineering, The National University of Singapore, Singapore.
Tsoi
Mandy M Y
MMY
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, People's Republic of China.
Li
Xiancui
X
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, People's Republic of China.
Plakhotnikova
Ioulia
I
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, People's Republic of China.
Dobretsov
Sergey
S
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, People's Republic of China.
Wu
Madeline C S
MCS
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, People's Republic of China.
Wong
Po-Keung
PK
Department of Biology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, People's Republic of China.
Qian
Pei-Yuan
PY
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, People's Republic of China.
D000818:Animals; D001482:Base Composition; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D008386:Marine Biology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014481:United States; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6