[Effects of perinatal exposure to bisphenol A inducing dopaminergic neuronal cell to apoptosis happening in midbrain of male rat offspring].
OBJECTIVE
To investigate the mechanism and effect of rat perinatal exposure to bisphenol A (BPA) resulting in midbrain dopaminergic neuronal cell apoptosis and tyrosine hydroxylase expression of male offspring.


METHODS
Rat dams were randomLy divided into 4 groups on gestational day(GD) 10 and given orally the bisphenol A doses as 0, 0.5, 5, 50 mg/kg x d from GD10 to weaning. The brains of male offspring were obtained for detecting, with immunohistochemistry protocol, the Caspase-3, Bcl-2 and tyrosine hydroxylase expression in the midbrain on postnatal day 21 or 30 respectively, and the midbrain apoptotic neuronal cell were detected by TUNEL on PND21.


RESULTS
The expression of Caspase-3 in the midbrain of rat male offspring were increased but bcl-2 were decreased on PND21 and 30, respectively. On PND21, apoptotic neuronal cell were found in the midbrain of high and medium doses groups. TH protein expression was decreased.


CONCLUSIONS
Perinatal exposure to bisphenol A can induce the apoptosis of midbrain dopaminergic neuron in the male rat offspring even after weaning, and concomitantly decrease the midbrain TH immunoreactivity, this may cause the abnormal function of dopaminergic pathway of rat male offspring.
Lin
Yong
Y
Department of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, China.
Zhang
Hao
H
Wang
Wen-dong
WD
Wu
De-sheng
DS
Jiang
Song-hui
SH
Qu
Wei-dong
WD
D000395:Air Pollutants, Occupational / Q000633:toxicity; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001559:Benzhydryl Compounds; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism*; D004968:Estrogens, Non-Steroidal / Q000633:toxicity; D005260:Female; D008297:Male; D008636:Mesencephalon / Q000473:pathology*; D009474:Neurons / Q000473:pathology; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis / Q000235:genetics; D000098826:Bisphenol A Compounds
D000395:Air Pollutants, Occupational; D001559:Benzhydryl Compounds; D004298:Dopamine; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D014446:Tyrosine 3-Monooxygenase; C006780:bisphenol A; D000098826:Bisphenol A Compounds