title: Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression.
abstract: The insertion element InsTipalpha was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach.
authors.affiliation: Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstrasse 5, D-91058 Erlangen, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Schlicht
authors.forename: Christian
authors.initials: C
authors.lastname: Berens
authors.forename: Janko
authors.initials: J
authors.lastname: Daam
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hillen
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D009154:Mutation; D010455:Peptides / Q000235:genetics* / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013753:Tetracycline Resistance / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; C470187:TetR(B) protein, E coli