title: Hoeflea alexandrii sp. nov., isolated from the toxic dinoflagellate Alexandrium minutum AL1V.
abstract: A Gram-negative, aerobic, non-symbiotic bacterium (AM1V30(T)) was isolated from the toxic dinoflagellate Alexandrium minutum AL1V. On the basis of 16S rRNA gene sequence similarity, strain AM1V30(T) was most closely related (97.4 % similarity) to the type strain of Hoeflea marina, which belongs to the family Phyllobacteriaceae within the order Rhizobiales of the class Alphaproteobacteria. A polyphasic approach was used to clarify the taxonomic position of strain AM1V30(T). During the course of this study, a second species was described by others as belonging to the genus Hoeflea, namely Hoeflea phototrophica; it showed a somewhat higher level of 16S rRNA gene sequence similarity with respect to strain AM1V30(T) (98.2 %) and was also taken into account. The fatty acid profiles, physiological and biochemical data and DNA G+C content (59.7 mol%) support the classification of strain AM1V30(T) as a member of the genus Hoeflea. The characteristics of the novel strain were sufficiently distinct to indicate that it represents a separate species. To confirm this conclusion, DNA-DNA hybridizations were performed: low values (between 15.8 and 29.8 %) were obtained in all cases. Thus, AM1V30(T) represents a novel species within the genus Hoeflea, for which the name Hoeflea alexandrii sp. nov. is proposed. Strain AM1V30(T) (=CECT 5682(T)=DSM 16655(T)) is the type strain.
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa', Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Palacios
authors.affiliation: Colección Española de Cultivos Tipo (CECT) and Departamento de Microbiología y Ecología, Universitat de València, 46100 Burjassot, València, Spain.
authors.forename: David R
authors.initials: DR
authors.lastname: Arahal
authors.affiliation: Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, PO Box 1552, 36200 Vigo, Pontevedra, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Reguera
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa', Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Irma
authors.initials: I
authors.lastname: Marín
mesh_terms: D000818:Animals; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry; D004141:Dinoflagellida / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D008386:Marine Biology; D008969:Molecular Sequence Data; D010802:Phylogeny; D020560:Proteobacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013030:Spain; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S