title: Effects of coffees before and after special treatment procedure on cell membrane potentials in stomach cells.
abstract: Coffee, one of the most excessively used beverages worldwide, commences the risk of gastroesophageal reflux (GER), which may lead to gastric ulcers and increase the risk of gastric cancer. Many attempts have been made by the coffee industry to diminish the irritating effect on mucosa by means of altering the extraction methods concerning gerbic acids and the roasting processes. This paper describes the effect of differently produced coffees involving two brands of Darboven and two brands of other coffee roasters. The aim of this study was to prove the results of gastric potential measurements we found in literature by using human AGS gastric epithelial cells (human adenocarcinoma). All four coffee extracts tested differentially affected the membrane resting potential of AGS cells. Coffees no. 1 and no. 2 depolarized the cells, presumably by increasing the cation entry into the cytosol. In marked contrast, coffee no. 4 hyperpolarizes the cells, possibly by H(+) extrusion and/or Cl(-) influx, suggesting that this coffee might increase acidity in the stomach, which might negatively affect the stomach, especially in people with gastroesophageal reflux symptoms. Overall, our data suggest that different roasting methods of coffees affect the membrane potentials of AGS stomach cells, resulting in increased influx of H+ possibly resulting in decreased stomach acidity and thus reducing GER. These results are in good accordance with clinical pharmacological results from potential difference measurements in healthy volunteers we found in the literature.
authors.affiliation: Department of Psychiatry, University of Freiburg Medical School, Freiburg, Germany. fiebich@vivacell.de
authors.forename: B L
authors.initials: BL
authors.lastname: Fiebich
authors.forename: P
authors.initials: P
authors.lastname: Valente
authors.forename: A
authors.initials: A
authors.lastname: Ferrer-Montiel
authors.forename: E
authors.initials: E
authors.lastname: Candelario-Jalil
authors.forename: J
authors.initials: J
authors.lastname: Menthe
authors.forename: P
authors.initials: P
authors.lastname: Luecker
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D002412:Cations / Q000032:analysis; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects; D003069:Coffee / Q000737:chemistry; D005744:Gastric Acid / Q000378:metabolism; D005745:Gastric Acidity Determination; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D010936:Plant Extracts / Q000494:pharmacology; D013270:Stomach / Q000166:cytology* / Q000187:drug effects; D013274:Stomach Neoplasms / Q000473:pathology
chemical_list: D002412:Cations; D003069:Coffee; D010936:Plant Extracts