Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions.
The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.
Dziekońska-Rynko
Janina
J
Department of Parasitology, Faculty of Biology, University of Warmia and Mazury, Zołnierska Str. 14, 10-561 Olsztyn.
Rokicki
Jerzy
J
Jabłonowski
Zbigniew
Z
D000818:Animals; D017163:Anisakis / Q000378:metabolism*; D002417:Cattle; D020536:Enzyme Activators / Q000378:metabolism*; D066298:In Vitro Techniques; D007814:Larva / Q000378:metabolism; D010206:Papain / Q000378:metabolism*; D010434:Pepsin A / Q000037:antagonists & inhibitors / Q000378:metabolism; D011480:Protease Inhibitors / Q000378:metabolism*; D013552:Swine; D014357:Trypsin / Q000378:metabolism*
D020536:Enzyme Activators; D011480:Protease Inhibitors; D014357:Trypsin; D010206:Papain; D010434:Pepsin A