title: Control of the innate epithelial antimicrobial response is cell-type specific and dependent on relevant microenvironmental stimuli.
abstract: Immune defence against microbes depends in part on the production of antimicrobial peptides, a process that occurs in a variety of cell types but is incompletely understood. In this study, the mechanisms responsible for the induction of cathelicidin and beta-defensin antimicrobial peptides were found to be independent and specific to the cell type and stimulus. Vitamin D3 induced cathelicidin expression in keratinocytes and monocytes but not in colonic epithelial cells. Conversely, butyrate induced cathelicidin in colonic epithelia but not in keratinocytes or monocytes. Distinct factors induced beta-defensin expression. In all cell types, vitamin D3 activated the cathelicidin promoter and was dependent on a functional vitamin D responsive element. However, in colonic epithelia butyrate induced cathelicidin expression without increasing promoter activity and vitamin D3 activated the cathelicidin promoter without a subsequent increase in transcript accumulation. Induction of cathelicidin transcript correlated with increased processed mature peptide and enhanced antimicrobial activity against Staphylococcus aureus. However, induction of beta-defensin-2 expression did not alter the innate antimicrobial capacity of cells in culture. These data suggest that antimicrobial peptide expression is regulated in a tissue-specific manner at transcriptional, post-transcriptional and post-translational levels. Furthermore, these data show for the first time that innate antimicrobial activity can be triggered independently of the release of other pro-inflammatory molecules, and suggest strategies for augmenting innate immune defence without increasing inflammation.
authors.affiliation: Division of Dermatology, University of California, San Diego and VA San Diego Healthcare System, San Diego, CA, USA.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schauber
authors.forename: Robert A
authors.initials: RA
authors.lastname: Dorschner
authors.forename: Kenshi
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Brook
authors.initials: B
authors.lastname: Brouha
authors.forename: Richard L
authors.initials: RL
authors.lastname: Gallo
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis* / Q000235:genetics; D001424:Bacterial Infections / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods; D002087:Butyrates / Q000494:pharmacology; D002478:Cells, Cultured; D002762:Cholecalciferol / Q000494:pharmacology; D003092:Colitis / Q000276:immunology; D004847:Epithelial Cells / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007113:Immunity, Innate; D007150:Immunohistochemistry / Q000379:methods; D015603:Keratinocytes / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017192:Skin Diseases, Bacterial / Q000276:immunology; D014162:Transfection / Q000379:methods; D023083:beta-Defensins / Q000096:biosynthesis* / Q000235:genetics; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D002087:Butyrates; C106641:DEFB4A protein, human; D023083:beta-Defensins; D002762:Cholecalciferol; D054804:Cathelicidins