T cell mediated immune responses in patients with tuberculous lymphadenitis from Butajira, southern Ethiopia.
The control of tuberculosis (TB) requires improved vaccines in addition to chemotherapy. It is essential to understand the immune response in tuberculosis to successfully evaluate potential vaccines. Current investigations have focused on immune responses in pulmonary forms. We studied the T-cell response of peripheral blood mononuclear cells (PBMC) from HIV-infected (n=8) and non-infected patients (n=19) with lymph node tuberculosis to PPD and short-term culture filtrates (ST-CF) of M. tuberculosis. PBMC from HIV-negative TB lymphadenitis patients proliferated in response to both antigens (p<0.001) and produced variably higher levels of IFN-gamma compared to healthy controls (p=0.02) (n=19) from the same area. Such responses were suppressed in HIV co-infected subjects. The results indicate that circulating PBMC in the apparently localized form of tuberculous lymphadenitis react to mycobacterial antigens in a similar pattern as those of patients with pulmonary disease.
Habte
Abebe
A
Armauer Hansen Research Institute, P.O. Box 1005, Addis Ababa, Ethiopia.
Geletu
Mulu
M
Olobo
Joseph Okao
JO
Kidane
Dawit
D
Negesse
Yohannes
Y
Yassin
Mohammed Ahmed
MA
Kifle
Bereda
B
Abate
Getahun
G
Harboe
Morten
M
Aseff
Abraham
A
D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005002:Ethiopia; D015658:HIV Infections / Q000150:complications / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*; D014388:Tuberculosis, Lymph Node / Q000150:complications / Q000276:immunology*
D000942:Antigens, Bacterial; D007371:Interferon-gamma