title: Salicylate induced tinnitus: behavioral measures and neural activity in auditory cortex of awake rats.
abstract: Neurophysiological studies of salicylate-induced tinnitus have generally been carried out under anesthesia, a condition that abolishes the perception of tinnitus and depresses neural activity. To overcome these limitations, measurement of salicylate induced tinnitus were obtained from rats using schedule induced polydipsia avoidance conditioning (SIPAC) and gap pre-pulse inhibition of acoustic startle (GPIAS). Both behavioral measures indicated that tinnitus was present after treatment with 150 and 250 mg/kg of salicylate; measurements with GPIAS indicated that the pitch of the tinnitus was near 16 kHz. Chronically implanted microwire electrode arrays were used to monitor the local field potentials and spontaneous discharge rate from multiunit clusters in the auditory cortex of awake rats before and after treatment with 150 mg/kg of salicylate. The amplitude of the local field potential elicited with 60 dB SPL tone bursts increased significantly 2h after salicylate treatment particularly at 16-20 kHz; frequencies associated with the tinnitus pitch. Field potential amplitudes had largely recovered 1-2 days post-salicylate when behavioral results showed that tinnitus was absent. The mean spontaneous spike recorded from the same multiunit cluster pre- and post-salicylate decreased from 22 spikes/s before treatment to 14 spikes/s 2h post-salicylate and recovered 1 day post-treatment. These preliminary physiology data suggest that salicylate induced tinnitus is associated with sound evoked hyperactivity in auditory cortex and spontaneous hypoactivity.
authors.affiliation: Center for Hearing and Deafness, Department of Communicative Disorders and Sciences, University at Buffalo, Buffalo, NY 14214, USA.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Edward
authors.initials: E
authors.lastname: Lobarinas
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhang
authors.forename: Jeremy
authors.initials: J
authors.lastname: Turner
authors.forename: Daniel
authors.initials: D
authors.lastname: Stolzberg
authors.forename: Richard
authors.initials: R
authors.lastname: Salvi
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
mesh_terms: D000818:Animals; D001303:Auditory Cortex / Q000187:drug effects* / Q000503:physiopathology; D001362:Avoidance Learning; D001522:Behavior, Animal; D003213:Conditioning, Psychological; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013216:Reflex, Startle; D012980:Sodium Salicylate / Q000633:toxicity*; D014012:Tinnitus / Q000139:chemically induced* / Q000503:physiopathology / Q000523:psychology
chemical_list: D012980:Sodium Salicylate