Interleukin-1 modulation of human placental trophoblast proliferation.
During early pregnancy, interleukin-1 (IL-1) is mainly produced and secreted by maternal decidua. Yet, its biological function on placental cells is not well defined. In this study, we employed JAR choriocarcinoma cell line as a model of human placental trophoblast to study the effect of IL-1. Treatment with recombinant human IL-1beta resulted in significant inhibition of JAR proliferation (P < .05) paralleled with increased cytotoxicity. The inhibitory effect was blocked by both IL-1 receptor antagonist (IL-1Ra) and antihuman IL-1beta monoclonal antibody. Analyzing the mode of action, IL-1beta was found to induce cell cycle arrest in the G0/G1 phase and triggered apoptotic cell death. These findings demonstrated that IL-1 regulates human trophoblast growth by induction of cell cycle delay and cell death.
Nilkaeo
Athip
A
Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkla 90112, Thailand.
Bhuvanath
Suthinee
S
D000911:Antibodies, Monoclonal / Q000378:metabolism; D017209:Apoptosis; D002453:Cell Cycle; D049109:Cell Proliferation; D016193:G1 Phase; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000494:pharmacology; D053583:Interleukin-1beta / Q000378:metabolism / Q000502:physiology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry; D016192:Resting Phase, Cell Cycle; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D011994:Recombinant Proteins; D007770:L-Lactate Dehydrogenase