title: Radiation hybrid map of the porcine genome comprising 2035 EST loci.
abstract: The IMpRH(7000-rad) radiation hybrid panel was used to map 2035 expressed sequence tags (ESTs) at a minimum LOD score of 4.0. A total of 134 linkage groups covers 57,192 cR or 78% of the predicted size of the porcine and 71% of the human genome, respectively. Approximately 81% (1649) of the porcine ESTs were annotated against the NCBI nonredundant database; 1422 mapped in silico to a location in build 35.1 of the human genome sequence (HGS) and 1185 to a gene and location in build 35.1 HGS. The map revealed 40 major breaks in synteny (1.00e (-25 )and lower) with the human genome, 37 of which fall within a single chromosome. At this improved level of resolution and coverage, porcine chromosomes (SSC) 2, 5, 6, 7, 12, and 14 remain "gene-rich" and homologous to human chromosomes (HSA) 17, 19, and 22, while SSC 1, 8, 11, and X have been confirmed to correspond to the "gene-deserts" on HSA 18, 4, 13, and X.
authors.affiliation: Department of Animal Biotechnology, College of Agriculture, Biotechnology and Natural Resources, University of Nevada, Reno, Nevada 89557, USA.
authors.forename: Anette
authors.initials: A
authors.lastname: Rink
authors.forename: Katie
authors.initials: K
authors.lastname: Eyer
authors.forename: Benjamin
authors.initials: B
authors.lastname: Roelofs
authors.forename: Kimberly J
authors.initials: KJ
authors.lastname: Priest
authors.forename: Kathleen J
authors.initials: KJ
authors.lastname: Sharkey-Brockmeier
authors.forename: Sudkamon
authors.initials: S
authors.lastname: Lekhong
authors.forename: Elif K
authors.initials: EK
authors.lastname: Karajusuf
authors.forename: Jason
authors.initials: J
authors.lastname: Bang
authors.forename: Martine
authors.initials: M
authors.lastname: Yerle
authors.forename: Denis
authors.initials: D
authors.lastname: Milan
authors.forename: Wan-Sheng
authors.initials: WS
authors.lastname: Liu
authors.forename: Craig W
authors.initials: CW
authors.lastname: Beattie
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D020224:Expressed Sequence Tags; D006801:Humans; D021101:Radiation Hybrid Mapping / Q000379:methods*; D013552:Swine / Q000235:genetics*; D026801:Synteny