[Comparison of two types of diagnostic test detecting antibodies against Borrelia burgdorferi: EIA based on one genospecies antigens and ELISA based on recombinant antigens].
The aim of the study was to compare the results of ELISA diagnostic kit detecting antibodies against B. burgdorferi based on combination of three genospecies recombinant and EIA kits based on one of three genospecies. Sera of 351 forest workers were evaluated with ELISA kit (Recombinant antigen, IgG). Seropositive samples were tested with EIA kits based on B. burgdorferi s.s., B. garinii, B. afzelii antigens. Diagnostic kits based on combination of antigens of three genospecies more often detect antibodies against B. burgdorferi and are more usefull as screening tests, in comparison with those based on one genospecies. Among diagnostic kits based on one genospecies, the most sesnitive in detection of antibodies against B. burgdorferi are those based on B. afzelii.
Zajkowska
Joanna
J
Klinika Chorób Zakaźnych i Neuroinfekcji, Akademii Medycznej w Białymstoku.
Kondrusik
Maciej
M
Grygorczuk
Sambor
S
Pancewicz
Sławomir
S
Swierzbińska
Renata
R
Sienkiewic
Iwona
I
Hermanowska-Szpakowicz
Teresa
T
D000382:Agricultural Workers' Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000145:classification*; D015748:Borrelia burgdorferi Group / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016468:Forestry; D006801:Humans; D007124:Immunoenzyme Techniques; D008193:Lyme Disease / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D008297:Male; D011044:Poland; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial