[Influence of different doses of porcine pulmonary on the therapeutic effects in rats with oleic acid induced acute lung injury].
OBJECTIVE
To investigate the therapeutic effects and dose effect relationship of intratracheal instillation of different doses of porcine pulmonary surfactant (PPS) in rats with oleic acid (OA) induced acute lung injury (ALI).


METHODS
Arterial blood gases and respiratory rate during the experiments, survival rate, lung index, total protein (TP) content in bronchoalveolar lavage fluid (BALF), tumor necrosis factor-alpha (TNF-alpha) level in plasma, light microscopy examination of lung specimens after the experiments were determined and performed in control group, OA (0.2 ml/kg) + saline, OA + PPS 50 mg/kg, OA+PPS 80 mg/kg, OA+PPS 100 mg/kg, OA+PPS 150 mg/kg, OA+PPS 200 mg/kg groups, respectively.


RESULTS
In PPS 50 mg/kg group, arterial blood gases were improved and respiratory rate was reduced during the first 2 hours (P<0.05). Arterial blood gases and reduced breath rates respiratory rate were not improved in other PPS treatment groups but 4 hours-survival rate was lowered, lung index was decreased, protein content s in BALF and TNF-alpha level in serum were lowered, and pathological changes were ameliorated compared with group given saline after OA, especially in high dosage of PPS (150-200 mg/kg) group (P<0.05).


CONCLUSIONS
Administration of PPS via trachea provides obvious effects on respiratory functions in rats with OA induced ALI, moreover PPS (> or =80 mg/kg) alleviates lung injury. There is no dose-effect relationship of PPS in PPS-treatment groups.
Sun
Yu
Y
Department of Pathophysiology, College of Basic Medical Science, The Second Military Medical University, Shanghai 200433, China.
Huang
Jing-xia
JX
Wang
Yi-qun
YQ
Zhong
Ji-gen
JG
Lu
Jian
J
D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D008168:Lung / Q000473:pathology; D008297:Male; D019301:Oleic Acid / Q000633:toxicity*; D011663:Pulmonary Surfactants / Q000008:administration & dosage* / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine
D011663:Pulmonary Surfactants; D019301:Oleic Acid