[HDMX amplification and high levels of HDMX-S splice variant are correlated with a poor prognosis in soft tissue sarcomas].
The aim of this study was to determine the amplification status of the HDMX gene and the expression of the HDMX mRNA particularly that of the HDMX-S splice variant in soft tissue sarcomas (STS). We show that the HDMX gene is amplified in 27% of STSs, which was associated with a worse prognosis (RR = 2.8, p = 0.03). We have also found that the transcript of the HDMX-S variant was predominant in a subset of (14%) of tumor samples, which was correlated with a significantly decreased overall survival time (15 vs. 53 months, p < 0.0001, log-Rank-test) and with a 9-fold-increased risk of tumor-related death (p < 0.0001). There was no correlation between the HMDX gene amplification and the HDMX-S splice variant overexpression. In summary, our data indicate that both the overexpression of the HDMX-S transcript, as well as, the HDMX gene amplification are important prognostic markers for STS.
Bartel
F
F
Institut für Pathologie, Martin-Luther-Universität Halle-Wittenberg.
Schulz
J
J
Blümke
K
K
Kappler
M
M
Bache
M
M
Schmidt
H
H
Taubert
H
H
D017398:Alternative Splicing; D018797:Cell Cycle Proteins; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005784:Gene Amplification; D005819:Genetic Markers; D014644:Genetic Variation; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D012509:Sarcoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016019:Survival Analysis; D014158:Transcription, Genetic
D018797:Cell Cycle Proteins; D017931:DNA Primers; D004273:DNA, Neoplasm; D005819:Genetic Markers; C482775:MDM4 protein, human; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm