title: Investigation of potential antibacterial properties of methanol extracts from fungus Ganoderma applanatum.
abstract: In this study we searched for antibacterial compounds in methanol extracts of three layers (tube layer, context and cutis) of Ganoderma applanatum fruit body against Bacillus subtilis ATCC 6051 as a gram-positive bacterium and Escherichia coli ATCC 25922 and Pseudomonas syringae pv. syringae DPIC 219 as gram-negative bacteria. We found antibacterial properties in all three layers only against gram-negative bacteria. The fractions were isolated and purified by thin layer chromatography (TLC) and high-performance liquid chromatography. After purification, four major compounds - A, B, C and D - were tested for antibacterial properties, and B and D were found to possess antibacterial properties. The results of TLC and spectral data, including mass spectra and (1)H NMR of compounds B and C, showed that they were saturated fatty acids and compound D was palmitic acid (hexadecanoic acid) (16:0).
authors.affiliation: Plant Protection Department, Faculty of Agriculture, Tehran University, Karaj, Iran.
authors.forename: Mohammad-Fata
authors.initials: MF
authors.lastname: Moradali
authors.forename: Hossien
authors.initials: H
authors.lastname: Mostafavi
authors.forename: Ghorban-Ali
authors.initials: GA
authors.lastname: Hejaroude
authors.forename: Abbas Sharifi
authors.initials: AS
authors.lastname: Tehrani
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Abbasi
authors.forename: Shirin
authors.initials: S
authors.lastname: Ghods
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D032902:Ganoderma / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D000432:Methanol / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D000432:Methanol