Activation of lymphocyte proliferation by boronate-containing polymer immobilised on substrate: the effect of boron content on lymphocyte proliferation.
This study demonstrates that boronic acid-containing polymers coated onto solid support function as synthetic mitogens for mouse lymphocytes. The polymer was synthesized by radical copolymerization of 3-acrylamidophenylboronic acid with dimethylacrylamide (poly(AAPBA-DMAA)). The boronic acid in the trigonal form in the copolymer activated lymphocytes, probably by crosslinkage to glycoprotein moieties on the plasma membrane surface, as in the case of lectin stimulation. A higher concentration of phenylboronic acid on the copolymer surface resulted in greater activation of lymphocytes, suggesting that the number of phenylboronic acid residues per unit area may be a crucial factor in lymphocyte proliferation. The proliferative response of lymphocytes was also affected by the surface wettability, probably due to a difference in the flexibility of polymer strands at the cell-polymer interface.
Otsuka
Hidenori
H
Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.
Ikeya
Takeshi
T
Okano
Teruo
T
Kataoka
Kazunori
K
D000180:Acrylic Resins / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D001897:Boronic Acids / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008934:Mitogens / Q000737:chemistry / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000180:Acrylic Resins; D001672:Biocompatible Materials; D001897:Boronic Acids; D008934:Mitogens; C513773:poly(AAPBA-DMAA)