title: Brevibacterium samyangense sp. nov., an actinomycete isolated from a beach sediment.
abstract: A novel actinomycete, strain SST-8(T), was isolated from sand sediment of Samyang Beach in Jeju, Korea, and subjected to a polyphasic taxonomic study. The organism, which produced opaque, circular, yellow colonies, with a coryneform morphology, showed the following chemotaxonomic characteristics: meso-diaminopimelic acid as the diamino acid in the peptidoglycan, MK-8(H(2)) as the major menaquinone, phosphatidylglycerol as the only polar lipid, anteiso-C(15 : 0) and anteiso-C(17 : 0) as major fatty acids and a DNA G+C content of 70.7 mol%. The combination of morphological and chemotaxonomic features supported its classification in the genus Brevibacterium. Phylogenetic analyses, based on 16S rRNA gene sequence studies, showed that strain SST-8(T) formed an intermediate branch between the Brevibacterium luteolum/Brevibacterium otitidis and Brevibacterium mcbrellneri/Brevibacterium paucivorans clusters. Sequence similarity calculations based on a neighbour-joining analysis revealed that the closest relatives of strain SST-8(T) were the type strains of B. paucivorans (96.6 %), B. luteolum (96.5 %), B. mcbrellneri (96.3 %), Brevibacterium avium (96.0 %) and B. otitidis (95.9 %). Based on a broad set of phenotypic and genetic data, it was evident that the strain represents a novel species of the genus Brevibacterium. The name Brevibacterium samyangense sp. nov. is proposed, with SST-8(T) (=NRRL B-41420(T)=KCCM 42316(T)) as the type strain.
authors.affiliation: Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
authors.forename: Soon Dong
authors.initials: SD
authors.lastname: Lee
mesh_terms: D001482:Base Composition; D001951:Brevibacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003487:Cyanoacrylates / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D007723:Korea; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010715:Phosphatidylglycerols / Q000032:analysis; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D024482:Vitamin K 2 / Q000031:analogs & derivatives
chemical_list: D003487:Cyanoacrylates; D004269:DNA, Bacterial; D010457:Peptidoglycan; D010715:Phosphatidylglycerols; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C007166:cyacrin; C024223:vitamin MK 8; D003960:Diaminopimelic Acid