Diagnostic tools based on minor groove binder probe technology for rapid identification of vaccinal and field strains of canine parvovirus type 2b.
TaqMan-based diagnostic tests have been developed for the identification of canine parvovirus type 2 (CPV-2) strains in the faeces of dogs with diarrhoea, including a minor groove binder (MGB) probe assay for identification of type 2-based vaccines and field strains (types 2a, 2b and 2c). Since type 2b vaccines have been licensed recently in Europe, two novel MGB assays were developed for discrimination between type 2b vaccines and field strains of CPV. Such assays have been found to be highly sensitive, specific and reproducible, allowing for simultaneous detection of type 2b vaccinal and field strains present in the same specimens. These new assays will help resolution of the diagnostic problems related to the detection of a type 2b strain in the faeces of dogs shortly after the administration of a type 2b vaccine.
Decaro
Nicola
N
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy. n.decaro@veterinaria.uniba.it
Martella
Vito
V
Elia
Gabriella
G
Desario
Costantina
C
Campolo
Marco
M
Buonavoglia
Domenico
D
Bellacicco
Anna Lucia
AL
Tempesta
Maria
M
Buonavoglia
Canio
C
D000704:Analysis of Variance; D000818:Animals; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology; D015336:Molecular Probe Techniques; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology*; D017993:Parvovirus, Canine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014765:Viral Vaccines
D004279:DNA, Viral; D014765:Viral Vaccines