Isolation from the rumen of a new acetogenic bacterium phylogenetically closely related to Clostridium difficile.
Five strains of filamentous acetogenic bacterium were isolated from high dilutions of ruminal content of newborn lambs. These Gram-positive spore-forming bacteria grew either chemolithotrophically with H2+ CO2 or chemo-organotrophically with glucose, cellobiose, fructose, maltose, mannose and syringic acid. The DNA base composition of the five strains were between 29.1 and 31.3 mol% G + C. Their temperature and pH optimum for growth were 35-40 degrees C and 6.5-7.0, respectively. The full 16S rRNA gene sequence analysis of the reference strain indicated that it was most closely related to Clostridium difficile. The sequence similarity value between the 16S rRNA gene of the reference strain and this pathogenic strain was 99.7%.
Rieu-Lesme
F
F
INRA, CR-Clermont-Ferrand-Theix, Saint-Genès-Champanelle, France. rieu@clermont.inra.fr
Dauga
C
C
Fonty
G
G
Dore
J
J