Pregnancy, bovine somatotropin, and dietary n-3 fatty acids in lactating dairy cows: III. Fatty acid distribution.
Our objective was to examine effects of exogenous bovine somatotropin (bST), pregnancy, and dietary fatty acids on fatty acid distribution in various tissues of lactating dairy cows. Two diets were fed, starting about 17 d in milk (DIM), in which oil of whole cottonseed (control diet) was compared with a calcium salt of fish oil-enriched lipid (FO; 1.9% of dietary DM). Starting at 44 +/- 5 DIM, ovulation was synchronized with a presynchronization plus Ovsynch protocol (d 0 = time of synchronized ovulation). Some cows were inseminated (77 +/- 12 DIM) to create a pregnant group. On d 0 and 11, cows received bST (500 mg) or no bST, and were killed on d 17 (94 +/- 12 DIM). Number of cows in control group was 5 bST-treated cyclic (bST-C), 5 non-bST-treated cyclic (no bST-C), 4 bST-treated pregnant (bST-P), and 5 non-bST-treated pregnant (no bST-P) cows; and for the FO diet: 4 bST-treated (bST-FO-C) and 5 non-bST-treated cyclic (no bST-FO-C) cows. At slaughter, samples of endometrium, liver, muscle, s.c. adipose, internal adipose, and mammary gland were collected. Milk was collected at 75 +/- 5 DIM. Gas chromatography was used to determine fatty acid percentages in tissues and milk fat. Endometrium from the cows fed FO had increased proportions of C20:5 and C22:6, whereas C20:4 was decreased. Injections of bST reduced both C18:2 and the n-6:n-3 ratio, but increased C22:6 in endometrium of cyclic control-fed, but not pregnant cows. In addition, FO decreased the n-6:n-3 ratio in all tissues and milk fat except for s.c. and internal adipose tissue. Cows fed FO also had increased C18:3, C20:5, and C22:6 in the liver and mammary tissue, and C18:3 and C22:6 were increased in the milk fat. The FO diet decreased the Delta9-desaturase index [(product of Delta9-desaturase]/(product of Delta9-desaturase + substrate of Delta9-desaturase]; DIX) in muscle and s.c. tissues, accompanied by an increase in saturated fatty acid (SFA) percentage. In addition, FO diet decreased DIX in the endometrium. In mammary and internal adipose tissues, bST increased DIX in cyclic control-fed cows, whereas bST decreased DIX in FO-fed cows, with no difference in the concentration of SFA and UNSFA. Cis-9, trans-11 conjugated linoleic acid was increased in milk fat, but decreased in the muscle and s.c. adipose tissue of FO-fed cows. The FO-enriched lipid, bST treatment, and early pregnancy can alter fatty acid percentages and distributions that may alter tissue functionality and functional nutrients of consumer products.
Bilby
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TR
Department of Animal Sciences, University of Florida, Gainesville, Florida 32611, USA.
Jenkins
T
T
Staples
C R
CR
Thatcher
W W
WW
D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004032:Diet / Q000662:veterinary*; D004717:Endometrium / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage / Q000378:metabolism*; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage / Q000378:metabolism; D013006:Growth Hormone / Q000008:administration & dosage / Q000378:metabolism*; D007774:Lactation / Q000502:physiology; D016018:Least-Squares Analysis; D008099:Liver / Q000737:chemistry; D008321:Mammary Glands, Animal / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D014018:Tissue Distribution
D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; C073294:growth hormone, bovine; D013006:Growth Hormone