title: Protection against adverse biological effects induced by space radiation by the Bowman-Birk inhibitor and antioxidants.
abstract: This study was undertaken to evaluate the protective effects of the soybean-derived Bowman-Birk inhibitor (BBI), BBI concentrate (BBIC) and/or antioxidants against the adverse biological effects induced by space radiation in cultured human epithelial cells. The effects of BBI, BBIC and a combination of ascorbic acid, co-enzyme Q10, L-selenomethionine (SeM) and vitamin E succinate on proton and HZE-particle [high-energy (high E) nuclei of heavier (high atomic number, Z) elements] radiation-induced cytotoxicity in MCF10 human breast epithelial cells and a phenotypic change associated with transformation in HTori-3 human thyroid epithelial cells were assessed with a clonogenic survival assay and a soft agar colony formation assay. The results demonstrate that BBIC and antioxidants are effective in protecting against space radiation-induced cytotoxicity in MCF10 cells and BBI, BBIC and antioxidants are effective in protecting against a space radiation-induced phenotypic change associated with transformation of HTori-3 cells.
authors.affiliation: Department of Radiation Oncology, the University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA. akennedy@mail.med.upenn.edu
authors.forename: Ann R
authors.initials: AR
authors.lastname: Kennedy
authors.forename: Zhaozong
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jeremiah J
authors.initials: JJ
authors.lastname: Donahue
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Ware
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D019610:Cytoprotection / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D011839:Radiation, Ionizing; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D014358:Trypsin Inhibitor, Bowman-Birk Soybean