Adiponectin deficiency does not affect the inflammatory response to endotoxin or concanavalin a in mice.
Adiponectin (APN) is an adipocyte-derived protein that regulates insulin sensitivity and displays antiinflammatory activities in a variety of experimental models. The present study aimed at investigating the effect of APN deficiency on the inflammatory response to endotoxin (lipopolysaccharide, LPS) and Concanavalin A (ConA) in vivo in mice. Administration of a high dose of LPS (100 microg/mouse) induced production of comparable amounts of IL-6, TNFalpha, and interferon-gamma in wild-type (WT) and APN knockout (KO) mice. Furthermore, LPS-induced hypoglycemia, anorexia, and body weight loss did not differ between WT and APN KO mice. Administration of a low dose of LPS (100 or 10 ng/g) in association with d-galactosamine induced equivalent mortality rates, hepatotoxicity, and serum IL-6 in WT and APN KO mice. Finally, ConA-induced cytokine production and hepatotoxicity were not significantly different between WT and APN KO mice. These data indicate that--despite its well-described role as an antiinflammatory molecule--endogenous APN does not play a critical role in modulating the inflammatory responses to LPS and ConA in mice.
Pini
Maria
M
Department of Human Nutrition, University of Illinois at Chicago, 1919 West Taylor Street, MC 517, Chicago, Illinois 60612, USA.
Sennello
Joseph A
JA
Chan
Lawrence
L
Fantuzzi
Giamila
G
D052242:Adiponectin / Q000097:blood / Q000172:deficiency / Q000502:physiology*; D000818:Animals; D003208:Concanavalin A / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay; D007249:Inflammation / Q000517:prevention & control*; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
D052242:Adiponectin; D008070:Lipopolysaccharides; D003208:Concanavalin A