title: Oncofetal protein glypican-3 in testicular germ-cell tumor.
abstract: The expression of an oncofetal protein, the glypican-3 (GPC3), was immunohistochemically evaluated in a wide variety of primary testicular germ-cell tumors (GCTs) in comparison with other markers, alpha-fetoprotein (AFP), human chorionic gonadotropin (hCG)-beta, and OCT3/4. Eighty-nine cases of GCT including 22 cases of mixed GCT were evaluated with reference to each tumor component. GPC3 expression was observed in neoplastic cells of yolk-sac tumor (YST) (25/25), teratoma (2/10), components of syncytiotrophoblastic giant cells (STGCs) (10/14), and choriocarcinoma (1/3), but none in intratubular germ-cell neoplasias, unclassified type (0/33), seminomas (0/61), or embryonal carcinoma (0/19). All cases of YST showed diffuse labeling of neoplastic cells in cytoplasmic and membranous patterns, and the positive area of GPC3 was much larger than that of AFP. Glandular structures in teratomas showed GPC3 immunostaining as well as AFP. Although the number of GPC3-positive cells was smaller in STGC components and choriocarcinoma, there was no diffusion artifact in GPC3 immunostaining, as was frequently encountered in hCG-beta staining. Thus, GPC3 is a unique oncofetal protein, which is useful as an immunohistochemical marker for GCT differentiated to extraembryonic tissue, especially YST.
authors.affiliation: Department of Pathology, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo, Tokyo, 113-0033, Japan. soota-path@umin.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ota
authors.forename: Michiyo
authors.initials: M
authors.lastname: Hishinuma
authors.forename: Naoko
authors.initials: N
authors.lastname: Yamauchi
authors.forename: Akiteru
authors.initials: A
authors.lastname: Goto
authors.forename: Teppei
authors.initials: T
authors.lastname: Morikawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Fujimura
authors.forename: Tadaichi
authors.initials: T
authors.lastname: Kitamura
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aburatani
authors.forename: Masashi
authors.initials: M
authors.lastname: Fukayama
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D002452:Cell Count; D053673:Glypicans / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009373:Neoplasms, Germ Cell and Embryonal / Q000378:metabolism* / Q000473:pathology; D012680:Sensitivity and Specificity; D013736:Testicular Neoplasms / Q000378:metabolism* / Q000473:pathology; D013737:Testis / Q000378:metabolism / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D053673:Glypicans; C031192:oncofetal antigens