title: Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus.
abstract: A novel sulphate-reducing bacterium (Ind 1) was isolated from a biofilm removed from a severely corroded carbon steel structure in a marine environment. Light microscopy observations revealed that cells were Gram-negative, rod shaped and very motile. Partial 16S rRNA gene sequencing and analysis of the fatty acid profile demonstrated a strong similarity between the new species and members from the Desulfovibrio genus. This was confirmed by the results obtained following purification and characterisation of the key proteins involved in the sulphate-reduction pathway. Several metal-containing proteins, such as two periplasmic proteins: hydrogenase and cytochrome c3, and two cytoplasmic proteins: ferredoxin and sulphite reductase, were isolated and purified. The latter proved to be of the desulfoviridin type which is typical of the Desulfovibrio genus. The study of the remaining proteins revealed a high degree of similarity with the homologous proteins isolated from Desulfovibrio gigas. However, the position of the strain within the phylogenetic tree clearly indicates that the bacterium is closely related to Desulfovibrio gabonensis, and these three strains form a separate cluster in the delta subdivision of the Proteobacteria. On the basis of the results obtained, it is suggested that Ind 1 belongs to a new species of the genus Desulfovibrio, and the name Desulfovibrio indonensis is proposed.
authors.affiliation: School of Biomedical and Pharmaceutical Sciences, University of Portsmouth, Portsmouth, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Feio
authors.forename: I B
authors.initials: IB
authors.lastname: Beech
authors.forename: M
authors.initials: M
authors.lastname: Carepo
authors.forename: J M
authors.initials: JM
authors.lastname: Lopes
authors.forename: C W
authors.initials: CW
authors.lastname: Cheung
authors.forename: R
authors.initials: R
authors.lastname: Franco
authors.forename: J
authors.initials: J
authors.lastname: Guezennec
authors.forename: J R
authors.initials: JR
authors.lastname: Smith
authors.forename: J I
authors.initials: JI
authors.lastname: Mitchell
authors.forename: J J
authors.initials: JJ
authors.lastname: Moura
authors.forename: A R
authors.initials: AR
authors.lastname: Lino