Inhibition of the TPA-induced cutaneous inflammation and hyperplasia by EC-SOD.
This study reports the roles of extracellular superoxide dismutase (EC-SOD) in the cutaneous inflammation and hyperplasia with 12-O-tetradecanoylphorbol-3-acetate (TPA) application in EC-SOD transgenic mice (Tg EC-SOD). Topical double TPA treatment induced the various inflammatory changes including the epidermal thickness, elevated the PCNA-labeling index, the edema formation, and increased production of hydrogen peroxide (H2O2) in wild type mice (WT). These changes were markedly suppressed in TPA-treated Tg EC-SOD. The expressions of the inflammatory cytokines, IL-1alpha and IL-1beta, were reduced in the TPA-treated Tg EC-SOD compared with those in TPA-treated WT. The expression of IL-1alpha was significantly increased in the skin of TPA-treated WT, especially in the basal and suprabasal layers, but it was restricted focally in basal layer of the skin of TPA-treated Tg EC-SOD. The number of infiltrating inflammatory cells and the IL-1beta expressing cells was obviously reduced in TPA-treated Tg EC-SOD in comparison with TPA-treated WT. The result suggests that EC-SOD might play an important role in the suppression of TPA-induced cutaneous inflammation and epidermal hyperplasia by regulating the expression of IL-1alpha and IL-1beta, although the mechanisms remain to be elucidated.
Ha
Hye-Yeong
HY
Department of Dermatology and Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-040, Republic of Korea.
Kim
Younghwa
Y
Ryoo
Zae Young
ZY
Kim
Tae-Yoon
TY
D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology / Q000517:prevention & control*; D006965:Hyperplasia / Q000139:chemically induced / Q000517:prevention & control*; D007375:Interleukin-1 / Q000096:biosynthesis*; D051379:Mice; D008822:Mice, Transgenic; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors*
D007375:Interleukin-1; C499090:Sod3 protein, mouse; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate