title: Production of reactive oxygen species in peripheral blood is increased in individuals with Helicobacter pylori infection and decreased after its eradication.
abstract: BACKGROUND
  Helicobacter pylori infection has been reported to cause gastroduodenal ulcer, mucosa-associated lymphoid tissue lymphoma, and gastric cancer. Recent studies have suggested that H. pylori infection may also associate with other diseases, including hematologic and dermatologic disorders, and cardiovascular injury, by unknown mechanisms.
  
  
  METHODS
  Production of reactive oxygen species (ROS) was determined in peripheral blood samples from 86 patients (34 H. pylori-negative and 52 H. pylori-positive subjects) using a highly sensitive chemiluminescence probe, L-012 (8-amino-5-chloro-7-phenylpyrido(3,4-d) pyridazine-1 and 4 (2H, 3H) dione). Eleven H. pylori-positive individuals were also analyzed their ROS production in peripheral blood after H. pylori eradication.
  
  
  RESULTS
  ROS production was significantly higher in individuals with H. pylori infection than in those without infection. Enhanced production of ROS was decreased significantly after eradication of H. pylori. No correlation was found between the extent of ROS production and sex, age, smoking status, alcohol ingestion, use of medications, or serum level of C-reactive protein.
  
  
  CONCLUSIONS
  These findings suggest that ROS production was enhanced in peripheral blood by H. pylori infection. Chemiluminescence analysis of blood samples using L-012 permits evaluation of systemic oxidative stress in patients with H. pylori infection.
authors.affiliation: Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, Osaka 545-8585, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mashimo
authors.forename: Manabu
authors.initials: M
authors.lastname: Nishikawa
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Higuchi
authors.forename: Masaki
authors.initials: M
authors.lastname: Hirose
authors.forename: Qian
authors.initials: Q
authors.lastname: Wei
authors.forename: Anwarul
authors.initials: A
authors.lastname: Haque
authors.forename: Eiji
authors.initials: E
authors.lastname: Sasaki
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Shiba
authors.forename: Kazunari
authors.initials: K
authors.lastname: Tominaga
authors.forename: Toshio
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Arakawa
authors.forename: Masayasu
authors.initials: M
authors.lastname: Inoue
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000428:Alcohol Drinking; D002097:C-Reactive Protein / Q000032:analysis; D016022:Case-Control Studies; D005260:Female; D016481:Helicobacter Infections / Q000097:blood* / Q000188:drug therapy / Q000473:pathology; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000097:blood*; D012737:Sex Factors; D012907:Smoking
chemical_list: D017382:Reactive Oxygen Species; D002097:C-Reactive Protein