Characterization of the telomere regions of scleractinian coral, Acropora surculosa.
Terminal ends of vertebrate chromosomes are protected by tandem repeats of the sequence (TTAGGG). First thought to be vertebrate specific, (TTAGGG)( n ) has recently been identified in several aquatic invertebrates including sea urchin (Strongylocentrotus purpuratus), bay scallop (Argopecten irradians), and wedgeshell clam (Donax trunculus). We analyzed genomic DNA from scleractinian corals, Acropora surculosa, Favia pallida, Leptoria phrygia, and Goniastrea retiformis to determine the telomere sequence. Southern blot analysis suggests the presence of the vertebrate telomere repeats in all four species. Treatment of A. surculosa sperm DNA with Bal31 exonuclease revealed progressive shortening of the DNA fragments positive for the (TTAGGG)(22) sequence, supporting location of the repeats at the chromosome ends. The presence of the vertebrate telomere repeats in corals is evidence that the (TTAGGG)( n ) sequence is highly conserved among a divergent group of vertebrate and invertebrate species. Corals are members of the Lower Metazoans, the group of organisms that span the gap between the fungi and higher metazoans. Corals are the most basal organism reported to have the (TTAGGG)( n ) sequence to date, which suggests that the vertebrate telomere sequence may be much older than previously thought and that corals may share a number of genes with their higher relatives.
Sinclair
Colleen S
CS
Department of Biological Sciences, Towson University, Towson, MD 21252, USA.
Richmond
Robert H
RH
Ostrander
Gary K
GK
D000818:Animals; D037421:Anthozoa / Q000235:genetics*; D015139:Blotting, Southern; D017124:Conserved Sequence / Q000235:genetics; D004706:Endodeoxyribonucleases; D006141:Guam; D013045:Species Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics*; D016615:Telomere / Q000235:genetics*
D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31