Rapid antibody screening by membrane chromatographic immunoassay technique.
This paper describes a novel membrane chromatographic immunoassay technique suitable for rapid screening of antibodies in serum samples. This technique could potentially be utilized for antibody screening in situations where screening for exposure to one of several possible antigens is required. A synthetic microporous membrane is first selectively loaded with antibodies from the test serum sample by hydrophobic interaction. The in situ affinity membrane thus formed is sequentially pulsed with the antigens corresponding to the antibodies being screened. From the antigen chromatogram peak profile thus obtained, inferences about the antibodies present in the serum sample can then easily be made. This technique in addition to being rapid and direct is conceptually simple, and does not use any expensive media or reagents. It would potentially be useful for rapid diagnosis of infections as well as for rapid assessment of conditions such as envenomation or exposure to toxic substances.
Ghosh
Raja
R
Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ont., Canada L8S 4L7. rghosh@mcmaster.ca
D000906:Antibodies / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D008567:Membranes, Artificial; D013056:Spectrophotometry, Ultraviolet
D000906:Antibodies; D008567:Membranes, Artificial