Temperature-induced cell detachment on immobilized pluronic surface.
The Pluronic F68 and F127, a triblock copolymer of ethylene oxide and propylene oxide, was activated using carbonyldiimidazole (CDI), and CDI-activated Pluronic F68 and F127 was subsequently immobilized on the surface of a poly-L-lysine-coated polystyrene tissue culture flask. Cell culture was performed on the Pluronic-immobilized flask. The morphology of fibroblasts (L929 cells) on the Pluronic F127-immobilized flask was mainly spherical, and showed less spreading behavior than that on the Pluronic F68-immobilized flask and conventional tissue culture flask. This observation indicates that L929 cells on Pluronic F127-immobilized flasks were cultured in a bio-inert environment. L929 cells were successively detached from both Pluronic F127-immobilized flask and Pluronic F68-immobilized flask by cooling the flask to 4-15 degrees C. This detachment is due to the hydration and dehydration properties of Pluronic, depending on the temperature. Umbilical cord blood was cultured in the Pluronic F127-immobilized and conventional polystyrene tissue culture flasks at 37 degrees C. The expression ratio of surface markers on hematopoietic stem cells (CD34 and CD133) cultured in the Pluronic F127-immobilized flask was significantly higher than that of the cells in polystyrene tissue culture flask.
Higuchi
Akon
A
Department of Materials and Life Science, Seikei University, 3-3-1 Kichijoji Kitamachi, Musashino, Tokyo 180-8633, Japan. higuchi@st.seikei.ac.jp
Aoki
Nobuo
N
Yamamoto
Taro
T
Miyazaki
Toyohiko
T
Fukushima
Hisashi
H
Tak
Tae Moon
TM
Jyujyoji
Shin
S
Egashira
Satsuki
S
Matsuoka
Yuki
Y
Natori
Shizue Hayashi
SH
D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D018952:Antigens, CD34 / Q000096:biosynthesis; D001672:Biocompatible Materials; D005312:Fetal Blood / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007093:Imidazoles / Q000737:chemistry*; D051379:Mice; D010455:Peptides; D020442:Poloxamer / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D014471:Umbilical Veins / Q000166:cytology
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D001672:Biocompatible Materials; D006023:Glycoproteins; D007093:Imidazoles; D010455:Peptides; D011137:Polystyrenes; C000606024:Prom1 protein, mouse; D020442:Poloxamer; D011107:Polylysine; C006900:N,N-carbonyldiimidazole