Phototoxicity of a core-modified porphyrin and induction of apoptosis.
A core-modified porphyrin, 5-phenyl-10,15-bis(carboxylatomethoxyphenyl)-20-(2-thienyl)-21,23-dithiaporphyrin (IY69) was studied in vitro for photodynamic activity under a variety of experimental protocols. Variables included the cell line (the rodent mammary tumor cell line R3230AC or the human breast cancer cell line MCF-7), light fluence, time of exposure of the cell cultures to IY69, and the time post-irradiation for cell counting. The length of time cell cultures were exposed to IY69 impacted cellular accumulation and cellular localization, phototoxicity, and the apparent mode of cell death - apoptosis vs. necrosis.
You
Youngjae
Y
Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY 14260-3000, United States. Youngjae.You@sdstate.edu <Youngjae.You@sdstate.edu>
Gibson
Scott L
SL
Detty
Michael R
MR
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D051379:Mice; D009336:Necrosis / Q000139:chemically induced*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D011166:Porphyrins / Q000737:chemistry / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C516937:5-phenyl-10,15-bis(carboxylatomethoxyphenyl)-20-(2-thienyl)-21,23-dithiaporphyrin; D017319:Photosensitizing Agents; D011166:Porphyrins