Tetrandrine concentrations not affecting oxidative phosphorylation protect rat liver mitochondria from oxidative stress.
The effects of tetrandrine (6,6', 7,12-tetramethoxy-2, 2'-dimethyl-berbaman) on the mitochondrial function were assessed on oxidative stress, mitochondrial permeability transition (MPT), and bioenergetics of rat liver mitochondria. At concentrations lower than 100 nmol/mg protein, tetrandrine decreased the hydrogen peroxide formation, the extent of lipid peroxidation, the susceptibility to Ca(2+)-induced opening of MPT pore, and inhibited the inner membrane anion channel activity, not significantly affecting the mitochondrial bioenergetics. High tetrandrine concentrations (100-300 nmol/mg protein) stimulated succinate-dependent state 4 respiration, while some inhibition was observed for state 3 and p-trifluoromethoxyphenylhydrazone-uncoupled respirations. The respiratory control ratio and the transmembrane potential were depressed but the adenosine diphosphate to oxygen (ADP/O) ratio was less affected. A slight increase of the inner mitochondrial membrane permeability to H(+) and K(+) by tetrandrine was also observed. It was concluded that low concentrations of tetrandrine afford protection against liver mitochondria injury promoted by oxidative-stress events, such as hydrogen peroxide production, lipid peroxidation, and induction of MPT. Conversely, high tetrandrine concentrations revealed toxicological effects expressed by interference with mitochondrial bioenergetics, as a consequence of some inner membrane permeability to H(+) and K(+) and inhibition of the electron flux in the respiratory chain. The direct immediate protective role of tetrandrine against mitochondrial oxidative stress may be relevant to clarify the mechanisms responsible for its multiple pharmacological actions.
Fernandes
Maria A S
MA
Departamento de Zoologia, Faculdade de Farmácia, Universidade de Coimbra, Couraça dos Apóstolos, 51, R/C, 3000-517 Coimbra, Portugal. mfer@ci.uc.pt
Custódio
José B A
JB
Santos
Maria S
MS
Moreno
António J M
AJ
Vicente
Joaquim A F
JA
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000633:toxicity*; D044182:Benzylisoquinolines / Q000494:pharmacology / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism; D019069:Cell Respiration / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects; D010085:Oxidative Phosphorylation; D018384:Oxidative Stress; D051381:Rats
D000975:Antioxidants; D044182:Benzylisoquinolines; C009438:tetrandrine; D006861:Hydrogen Peroxide; D002118:Calcium