Diversified serum IgG response involving non-myelin CNS proteins during experimental autoimmune encephalomyelitis.
We sequentially analyzed the serum IgG response against normal mouse brain during experimental autoimmune encephalomyelitis in SJL/J mice injected with CFA, Bordetella pertussis toxin (BPT) and proteolipid protein 139-151 peptide, compared with mice that received CFA and BPT or were uninjected. Dynamic changes were observed from day 0 to day 28 in the 3 groups. Six highly discriminant antigenic bands (kappa=0.974) were identified. Three non-myelin proteins were characterized (mitochondrial aconitase hydratase 2, phosphoglycerate mutase 1, brain specific pyruvate deshydrogenase). The IgG response against two of them was less frequent in EAE whereas it was associated with multiple sclerosis in our previous work.
Zephir
Helene
H
Laboratoire d'Immunologie EA2686, Faculté de Médecine, 1, Place de Verdun, Lille Cedex, France. H-ZEPHIR@chru-lille.fr
Almeras
Lionel
L
El Behi
Mohamed
M
Dussart
Patricia
P
de Seze
Jerome
J
Steibel
Jerome
J
Trifilieff
Elizabeth
E
Dubucquoi
Sylvain
S
Dessaint
Jean-Paul
JP
Vermersch
Patrick
P
Prin
Lionel
L
Lefranc
Didier
D
D000154:Aconitate Hydratase / Q000276:immunology; D000818:Animals; D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000276:immunology*; D015153:Blotting, Western; D001921:Brain / Q000276:immunology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000097:blood* / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood*; D051379:Mice; D018991:Myelin Proteolipid Protein / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D010736:Phosphoglycerate Mutase / Q000276:immunology; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001323:Autoantibodies; D001324:Autoantigens; D007074:Immunoglobulin G; D018991:Myelin Proteolipid Protein; D010446:Peptide Fragments; D011768:Pyruvate Dehydrogenase Complex; C435023:myelin proteolipid protein (139-151); D000154:Aconitate Hydratase; D010736:Phosphoglycerate Mutase