Use of a putative transcriptional regulator gene as target for specific identification of Staphylococcus epidermidis.
OBJECTIVE
To investigate the use of a Staphylococcus epidermidis transcriptional regulator gene as target for species-specific determination.


RESULTS
Staph. epidermidis genes encoding putative transcriptional regulators were retrieved from GenBank and those showing no homology with other bacterial sequences were selected. Of the four PCR primer sets analysed, the primers Serp0107F/R from serp0107 amplified a specific product of 581 bp from Staph. epidermidis DNA only, and they did not cross-react in PCR with nonepidermidis staphylococci and other common bacteria.


CONCLUSIONS
Being uniquely present in Staph. epidermidis, putative transcriptional regulator gene serp0107 offers a valuable target for specific identification of Staph. epidermidis.


CONCLUSIONS
As a member of a specialized gene group, putative transcriptional regulator gene serp0107 may be important to Staph. epidermidis adaptation to its niche environment. Further analysis of serp0107 and its related protein may help reveal new insights on the molecular regulation of Staph. epidermidis survival and virulence.
Liu
D
D
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA. liu@cvm.msstate.edu
Swiatlo
E
E
Austin
F W
FW
Lawrence
M L
ML
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D005809:Genes, Regulator; D016133:Polymerase Chain Reaction; D013212:Staphylococcus epidermidis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014158:Transcription, Genetic
D004269:DNA, Bacterial