Supercritical fluid extraction of limonoid glucosides from grapefruit molasses.
Limonoid glucosides (primarily limonin 17-beta-D-glucopyranoside, LG) were extracted from grapefruit molasses by supercritical fluid extraction using a supercritical carbon dioxide-ethanol (SC CO(2)-ethanol) system. Extraction conditions to maximize the yield of LG were determined by varying pressure, temperature, ethanol concentration, and extraction time. The highest yield of LG at 0.61 mg/g molasses was obtained at a pressure 48.3 MPa, a temperature of 50 degrees C, 10% ethanol (X(Eth) = 0.1), and 40 min of extraction time at a flow rate of 5.0 L/min. The results demonstrated that SC CO(2) extraction of limonoid glucosides from grapefruit molasses has practical significance for commercial production.
Yu
Jun
J
Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, 77845, USA.
Dandekar
Deepak V
DV
Toledo
Romeo T
RT
Singh
Rakesh K
RK
Patil
Bhimanagouda S
BS
D025924:Chromatography, Supercritical Fluid; D032083:Citrus paradisi / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification*; D036701:Limonins / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry
D005960:Glucosides; D036701:Limonins; D010936:Plant Extracts