Functional genomics: applying calcium imaging and RNA interference to Drosophila cell lines to identify new roles for gene products.
The selective knockdown of genes using RNA interference, and the analysis of the resultant phenotype using calcium imaging, is proving to be a powerful combination for discovering new genes involved in calcium signalling. This technical note describes the application of this approach to the Drosophila S2 cell line and its derivatives.
Buckingham
Steven D
SD
MRC Functional Genetics Unit, Department of Physiology, Anatomy and Genetics Le Gros Clark Building, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK. steven.buckingham@human-anatomy.oxford.ac.uk
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003952:Diagnostic Imaging; D004330:Drosophila / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D008264:Macrophages / Q000378:metabolism*; D034622:RNA Interference
D002118:Calcium