[Use of the PCR technique for diagnosis of symptomatic congenital toxoplasmosis based on own observations].
The aim of our study was to determine the usefulness of PCR technique in postnatal diagnosis of congenital toxoplasmosis in 17 infants aged from 1 week to 10 months. In 13 cases the diagnosis was established. The diagnostic procedures involved: clinical examinations, serological tests, nested-PCR method and neuroimaging examinations.


RESULTS
In almost all children (except one) a high level of toxoplasmatic IgG antibodies was found; in 1/3 we identified antibodies IgM and IgA. In 14 cases the PCR test from CSF was positive. In 4 infants the suspicion of congenital toxoplasmosis was ruled out; in 2 of them PCR from CSF was negative, in 2 - a false positive result was stated.


CONCLUSIONS
PCR is a valuable technique for diagnosis of congenital toxoplasmosis. Because of the possibility of false positive and negative results - it should be combined with other laboratory methods.
Lipka
B
B
Klinika Niemowleca lnstytutu "Pomnik - Centrum Zdrowia Dziecka", 04-736 Warszawa Al. Dzieci Polskich 20.
Milewska-Bobula
B
B
Kapusta
M
M
D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D016054:DNA, Protozoan / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis*; D012698:Serologic Tests / Q000379:methods; D014122:Toxoplasma / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000175:diagnosis; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000469:parasitology
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan