title: Maintained ENaC trafficking in aldosterone-infused rats during mineralocorticoid and glucocorticoid receptor blockade.
abstract: Aldosterone induces redistribution of epithelial sodium channel (ENaC) to the apical plasma membrane from intracellular vesicles in renal connecting tubule (CNT) and cortical collecting duct (CCD). The role of the classical mineralocorticoid receptor (MR) in ENaC trafficking is still debated. We examined whether the MR antagonist spironolactone affects ENaC regulation in the kidney cortex of aldosterone-infused rats. Aldosterone infusion for 7 days resulted in a plasma aldosterone concentration in the high physiological range (3 to 4 nM). Aldosterone infusion decreased plasma K(+) concentration compared with untreated control rats. Cotreatment with spironolactone completely blocked the aldosterone-induced decrease in plasma K(+). Immunoblotting and immunohistochemistry showed increased protein abundance of Na-K-ATPase alpha(1)-subunit and NCC in the kidney cortex, in response to aldosterone infusion that was blocked by spironolactone. In contrast, aldosterone-induced redistribution of ENaC subunits from the cytoplasm to the apical plasma membrane domain in CNT and CCD was unaffected by spironolactone. Immunoblotting of alphaENaC showed increased protein abundance in aldosterone-infused rats that was not blocked by spironolactone treatment. To exclude possible glucocorticoid receptor (GR)-mediated effects of aldosterone, we treated aldosterone-infused rats with both spironolactone and the GR antagonist RU486. Combined MR and GR blockade prevented neither ENaC trafficking nor the upregulation of alphaENaC protein abundance in aldosterone-infused rats. We provide new evidence for ENaC trafficking occurring independent of MR and GR activation in aldosterone-infused rats.
authors.affiliation: The Water and Salt Research Center, Institute of Anatomy, Aarhus, Denmark.
authors.forename: Jakob
authors.initials: J
authors.lastname: Nielsen
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Kwon
authors.forename: Jørgen
authors.initials: J
authors.lastname: Frøkiaer
authors.forename: Mark A
authors.initials: MA
authors.lastname: Knepper
authors.forename: Søren
authors.initials: S
authors.lastname: Nielsen
mesh_terms: D000450:Aldosterone / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D004326:Drinking / Q000502:physiology; D053503:Epithelial Sodium Channels / Q000096:biosynthesis* / Q000235:genetics; D006727:Hormone Antagonists / Q000494:pharmacology; D015151:Immunoblotting; D007150:Immunohistochemistry; D007262:Infusions, Intravenous; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D015735:Mifepristone / Q000494:pharmacology; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D011188:Potassium / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors*; D012982:Sodium, Dietary / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis; D013148:Spironolactone / Q000494:pharmacology
chemical_list: D053503:Epithelial Sodium Channels; D006727:Hormone Antagonists; D000451:Mineralocorticoid Receptor Antagonists; D011965:Receptors, Glucocorticoid; D012982:Sodium, Dietary; D013148:Spironolactone; D015735:Mifepristone; D000450:Aldosterone; C499076:ATP1A1 protein, human; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium