Effects of Safflower seed oil in osteoporosis induced-ovariectomized rats.
The effects of Korean Safflower (Carthamus tinctorius L) seed oil (SSO) on osteoporosis induced-ovariectomized rats were investigated. A total of 90 female Sprague-Dawley rats, 4-month-old, weighing 200-230 g were randomly assigned into 3 groups (30 animals/group). The animals in group I were sham operated and those of group II and III were ovariectomized (Ovx). After eight weeks, the animals of group I and II received solvent vehicle daily, whereas those of group III were administered SSO orally (1 ml/kg) daily for 30 days. The changes in the serum levels of insulin-like growth factor-I (IGF-I), IGF-II, insulin-like growth factor binding protein-3 (IGBP-3), estrogen, total alkaline phosphatase (TALP), bone-specific alkaline phosphatase (BALP), calcium and phosphorous in serum, and also the histomorphology of the proximal tibia metaphysis and femur/body weight (F/B) ratio were examined in all the groups at every 10 days interval. Thirty days later, IGF-I, IGF-II, IGBP-3 and BALP levels were significantly increased (p < 0.05) in group III as compared to groups I and II. There were no significant differences in serum levels of estrogen, TALP and F/B ratio between groups II and III, but estrogen levels were higher in group I. These results suggest that the safflower seeds have possible roles in the improvement of osteoporosis induced-ovariectomized rats.
Alam
Md Rafiqul
MR
Bio-safety Research Institute, Department of Surgery, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Kim
Soo Mi
SM
Lee
Jong Il
JI
Chon
Seung Ki
SK
Choi
Sung Jin
SJ
Choi
In Hyuk
IH
Kim
Nam Soo
NS
D000284:Administration, Oral; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D002118:Calcium / Q000097:blood; D004967:Estrogens / Q000097:blood; D005260:Female; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D010024:Osteoporosis / Q000097:blood* / Q000517:prevention & control; D010052:Ovariectomy; D010758:Phosphorus / Q000097:blood; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012450:Safflower Oil / Q000008:administration & dosage / Q000494:pharmacology*
D004967:Estrogens; D018972:Insulin-Like Growth Factor Binding Protein 3; D010758:Phosphorus; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D012450:Safflower Oil; D000469:Alkaline Phosphatase; D002118:Calcium