[BRG1 expression in prostate carcinoma by application of tissue microarray].
OBJECTIVE
To investigate the expression of gene BRG1 in prostatic intraepithelial neoplasia and adenocarcinoma, and the relationship between gene BRG1 expression and the clinicopathological features of prostate carcinoma.


METHODS
Gene BRG1 expression was evaluated in 37 cases of human prostate carcinoma, 13 human prostatic intraepithelial neoplasia (PIN) and 14 human benign prostatic hyperplasia (BPH) by using immunohistochemistry (EnVision method) and tissue microarray.


RESULTS
The positive rates of BRG1 protein were 81.08% (30/37), 38.46% (5/13) and 14.28% (2/14) in prostate carcinoma, PIN and BPH, respectively, significantly higher in the first group than in the latter two (P < 0.05). There was no statistically significant difference in BRG1 gene expression either between PIN and BPH (P > 0.05) or between the groups of the moderate differentiation (the Gleason histologic grading: 5-7) and the lower one (the Gleason histologic grading: 8-10) (P > 0.05).


CONCLUSIONS
BRG1 may play an important role in the development of prostate carcinoma. Tissue microarray technology, with the advantages of high throughput, conciseness, rapidity, high efficiency, low cost, and nice reproducibility, has significant practical value and broad application prospects in pathology.
Li
Yan
Y
Department of Pathology, Clinical School of Nanjing University Medical College, Nanjing General Hospital of Nanjing Command, Nanjing, Jiangsu 210002, China.
Shi
Qun-li
QL
Jin
Xing-zao
XZ
Meng
Kui
K
Zhou
Xiao-jun
XJ
Sun
Li-ping
LP
D000368:Aged; D000369:Aged, 80 and over; D004265:DNA Helicases / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D046208:Microchip Analytical Procedures; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D015203:Reproducibility of Results; D014157:Transcription Factors / Q000096:biosynthesis*
D009687:Nuclear Proteins; D014157:Transcription Factors; C084108:SMARCA4 protein, human; D004265:DNA Helicases