[Detection of rpoB mutations in rifampicin-resistant Mycobacterium kansasii].
OBJECTIVE
To detect rifampicin-resistant mutations in Mycobacterium kansasii (M. kansasii).


METHODS
We examined the M. kansasii isolates from sputum of patients at National Hospital Organization Kinki-chuo Chest Medical Center from January 1, 2001 to November 30, 2005 using drug-susceptibility testing, and analyzed 69-bp fragment of rpoB gene in rifampicin-resistant strains.


RESULTS
Three strains from 314 isolates were determined as rifampicin resistant using drug-susceptibility testing. Those strains showed a rise in minimum inhibitory concentration (MIC), and had the mutations in rpoB gene. These point mutations in codons 513 and 516 were common mutations found in rifampicin-resistant clinical isolates of M. tuberculosis.


CONCLUSIONS
We verified the association between rpoB gene mutations and rifampicin resistance in M. kansasii.
Yoshida
Shiomi
S
Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, 1180 Nagasone-cho, Kita-ku, Sakai-shi, Osaka 591-8555 Japan. dustin@kch.hosp.go.jp
Suzuki
Katsuhiro
K
Tsuyuguchi
Kazunari
K
Iwamoto
Tomotada
T
Tomita
Motohisa
M
Okada
Masaji
M
Sakatani
Mitsunori
M
D024881:Drug Resistance, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D009154:Mutation; D019909:Mycobacterium kansasii / Q000187:drug effects / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology*
D012293:Rifampin