Nitric oxide-independent lipid metabolism in RAW 264.7 macrophages loaded with oleic acid.
The role of nitric oxide (NO) in the regulation of lipogenesis and lipolysis in RAW 264.7 macrophages loaded with oleic acid (OA) was investigated in this paper. Magnolol stimulated full lipolysis without affecting NO levels. Both inhibition and elevation of NO production in OA-loaded macrophages did not induce lipolysis. Besides, lipopolysaccharide (LPS)-induced increased accumulation of lipid droplets was not reduced by down-regulation of NO levels. Moreover, incubation of macrophages with sodium nitroprusside (SNP), an NO donor, stimulated significant NO production without altering the lipid droplet accumulation. All these results clearly demonstrate that NO is not involved in the modulation of lipid metabolism in macrophages loaded with OA.
Huang
Wen-Chen
WC
Division of Plastic Surgery, Department of Surgery, Mackay Memorial Hospital, Taipei 110, Taiwan, ROC.
Chen
Jin-Shan
JS
D000818:Animals; D002460:Cell Line; D008066:Lipolysis / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism*; D019301:Oleic Acid / Q000494:pharmacology*; D010078:Oxazines / Q000378:metabolism
D008070:Lipopolysaccharides; D010078:Oxazines; D019301:Oleic Acid; D009569:Nitric Oxide; C044808:nile red