Exotic flora dependence of an unusual Brazilian propolis: the pinocembrin biomarker by capillary techniques.
Significant amounts of pinocembrin (>10%), a dihydroxy-flavanone, was found in the composition of an unusual brand of a subtropical Brazilian propolis. Incidentally, this sealing material was obtained from hives surrounding a large forestry site based on a single exotic flora, namely poplar (Populus sp.). Examination of the different botanical parts of poplar revealed the buds as the main source of the flavanone. Techniques used for the establishment of the chemical correlation between the propolis brand and the poplar buds were TLC/densitometry, capillary GC-MS in the e.i. mode, and CZE with DAD monitoring. Since color enhancement after Al3+ complexation applies just for more hydroxylated flavonoids, the alternative techniques herein applied were of value for pinocembrin detection and estimation. Analytical data indicated the dominance of the main phenolic pinocembrin biomarker as well as the presence of other related flavonoids in the botanical source and in the propolis derived thereof.
Adelmann
Juliana
J
Biomass Chemo/Biotechnology Laboratory (LQBB), Department of Pharmacy, Federal University of Parana, R. Lothario Meissner 632, Subsede Botânico, Curitiba PR (80210-170), Brazil.
Passos
Mauricio
M
Breyer
Daniel Henrique
DH
dos Santos
Maria Helena Rocha
MH
Lenz
Cesar
C
Leite
Natalício Ferreira
NF
Lanças
Fernando Mauro
FM
Fontana
José Domingos
JD
D000535:Aluminum / Q000737:chemistry; D000818:Animals; D001516:Bees; D015415:Biomarkers; D001938:Brazil; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002855:Chromatography, Thin Layer; D003720:Densitometry; D019075:Electrophoresis, Capillary; D044950:Flavanones / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D032107:Populus / Q000737:chemistry*; D011429:Propolis / Q000737:chemistry*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D015415:Biomarkers; D044950:Flavanones; D005419:Flavonoids; D007202:Indicators and Reagents; C016063:pinocembrin; D011429:Propolis; D000535:Aluminum