Short-term heart retention and distribution of intramyocardial delivered mesenchymal cells within necrotic or intact myocardium.
Cell therapy with bone marrow mesenchymal stem cells (BMSCs) is a new strategy for treating ischemic heart failure, but data concerning the distribution and retention of transplanted cells remain poor. We investigated the short-term myocardial retention of BMSCs when these cells are directly injected within necrotic or intact myocardium. 111Indium-oxine-labeled autologous BMSCs were injected within either 1-month-old infarction (n = 6) or normal myocardium (n = 6) from rats. Serial in vivo pinhole scintigraphy was scheduled during 1 week in order to track the implanted cells. The myocardial retention of BMSCs was definitely higher in myocardial infarction than in normal myocardial area (estimated percent retention at 2 h: 63 +/- 3% vs. 25 +/- 4%, p < 0.001) and the estimated cardiac retention values were unchanged in both groups along the 7 days of follow-up. On heart sections at day 7, labeled BMSCs were still around the injection site and appeared confined to the scarred tissue corresponding either to the infarct area or to the myocardium damaged by needle insertion. BMSCs have a higher retention when they are injected in necrotic than in normal myocardial areas and these cells appear to stay around the injection site for at least a 7-day period.
Tran
Nguyen
N
Laboratory of Surgery School, Faculty of Medicine-Nancy, Avenue de la forêt de Haye, BPl84, 54505 Vandoeuvre-lès-Nancy Cedex, France. Nguyen.Tran@medecine.uhp-nancy.fr
Li
Yan
Y
Maskali
Fatiha
F
Antunes
Laurent
L
Maureira
Pablo
P
Laurens
Marie-Helene
MH
Marie
Pierre-Yves
PY
Karcher
Gilles
G
Groubatch
Frederique
F
Stoltz
Jean-François
JF
Villemot
Jean-Pierre
JP
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000000981:diagnostic imaging; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000000981:diagnostic imaging; D008856:Microscopy, Fluorescence; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery; D009206:Myocardium / Q000473:pathology*; D009336:Necrosis; D011877:Radionuclide Imaging; D051381:Rats; D013997:Time Factors; D014182:Transplantation, Autologous