The potato transcriptional co-activator StMBF1 is up-regulated in response to oxidative stress and interacts with the TATA-box binding protein.
To gain a better understanding on the function of the potato Solanum tuberosum Multiprotein Bridging Factor 1 protein (StMBF1) its interaction with the TATA box binding protein (TBP) was demonstrated. In addition we reported that StMBF1 rescues the yeast mbf1 mutant phenotype, indicating its role as a plant co-activator. These data reinforce the hypothesis that MBF1 function is also conserved among non closely related plant species. In addition, measurement of StMBF1 protein level by Western blot using anti-StMBF1 antibodies indicated that the protein level increased upon H(2)O(2) and heat shock treatments. However, the potato beta-1,3-glucanase protein level was not changed under the same experimental conditions. These data indicate that StMBF1 participates in the cell stress response against oxidative stress allowing us to suggest that MBF1 genes from different plant groups may share similar functions.
Arce
Débora Pamela
DP
Instituto de Investigaciones Biologicas, FCEyN, UNMDP, CP 7600 Mar del Plata, Buenos Aires, Argentina.
Tonón
Claudia
C
Zanetti
María Eugenia
ME
Godoy
Andrea Verónica
AV
Hirose
Susumu
S
Casalongué
Claudia Anahí
CA
D018869:Heat-Shock Response; D006861:Hydrogen Peroxide / Q000633:toxicity; D018384:Oxidative Stress; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism; D035181:TATA-Box Binding Protein / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D015854:Up-Regulation; D015003:Yeasts
D010940:Plant Proteins; D035181:TATA-Box Binding Protein; D015534:Trans-Activators; D006861:Hydrogen Peroxide