Production of resveratrol in recombinant microorganisms.
Resveratrol production in Saccharomyces cerevisiae was compared to that in Escherichia coli. In both systems, 4-coumarate:coenzyme A ligase from tobacco and stilbene synthase from grapes were expressed. When p-coumaric acid was used as the precursor, resveratrol accumulations in the culture medium were observed to be comparable in E. coli (16 mg/liter) and yeast (6 mg/liter).
Beekwilder
Jules
J
Plant Research International, 6700 AA Wageningen, The Netherlands. Jules.Beekwilder@wur.nl
Wolswinkel
Rianne
R
Jonker
Harry
H
Hall
Robert
R
de Vos
C H Ric
CH
Bovy
Arnaud
A
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism; D003373:Coumaric Acids / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic; D000077185:Resveratrol; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D013267:Stilbenes / Q000378:metabolism*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics; D027843:Vitis / Q000235:genetics / Q000378:metabolism
D003373:Coumaric Acids; D013267:Stilbenes; D000217:Acyltransferases; C022674:stilbene synthase; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase; D000077185:Resveratrol