[Expression of various matrix metalloproteinases in mice with hyperoxia-induced acute lung injury].
OBJECTIVE
To investigate the role of matrix metalloproteinases (MMPs) and extracellular matrix metalloproteinase inducer (EMMPRIN) in the pathogenesis of acute lung injury induced by hyperoxia.


METHODS
Fifty four mice were exposed in sealed cages to >98% oxygen (for 24-72 hours), and another 18 mice to room air. The severity of lung injury was assessed, and the expression of mRNA and protein of MMP-2, MMP-9 and EMMPRIN in lung tissue, after exposure for 24, 48 and 72 hours of hyperoxia were studied by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry.


RESULTS
Hyperoxia caused acute lung injury; this was accompanied by increased expression of an upregulation of MMP-2, MMP-9 and EMMPRIN mRNA and protein in lung tissues.


CONCLUSIONS
Hyperoxia causes acute lung injury in mice; increases in MMP-2, MMP-9 and EMMPRIN may play an important role in the development of hyperoxia induced lung injury in mice.
Zhang
Xiang-feng
XF
Department of Pulmonary Medicine, Beijing Anzhen Hospital, Beijing 100029, China. xfzh119@hotmail.com
Ding
Shao-fang
SF
Gao
Yuan-ming
YM
Liang
Ying
Y
Foda
Hussein D
HD
D055371:Acute Lung Injury / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D018496:Hyperoxia / Q000150:complications* / Q000378:metabolism; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
C497887:Bsg protein, mouse; D012333:RNA, Messenger; D051926:Basigin; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse