T cells traffic from brain to cervical lymph nodes via the cribroid plate and the nasal mucosa.
Although drainage pathways of soluble antigens from brain to cervical lymph nodes have been well established, there is no direct evidence for similar routes of leukocytes leaving the central nervous system. We developed a protocol allowing the cross-sectioning of an entire head-neck preparation while preserving the signal of the GFP. We monitored how GFP-expressing CD4 T lymphocytes injected into the entorhinal cortex after lesion or the lateral ventricle of unlesioned C57/bl6 mice reach cervical lymph nodes. Irrespective of the injection site, we demonstrate their passage through the cribroid plate, appearance in the nasal mucosa, and specific accumulation in one of the cervical lymph nodes.
Goldmann
Jana
J
Department of Experimental Neuroimmunology, Charité, Universitätsmedizin, Berlin, Germany.
Kwidzinski
Erik
E
Brandt
Christine
C
Mahlo
Jacqueline
J
Richter
Daniel
D
Bechmann
Ingo
I
D000818:Animals; D001921:Brain / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D004195:Disease Models, Animal; D005004:Ethmoid Bone / Q000276:immunology*; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009297:Nasal Mucosa / Q000276:immunology*; D009333:Neck
D049452:Green Fluorescent Proteins