title: Does a deficiency of the signal recognition particle (SRP)-pathway affect the biosynthesis of its components in Saccharomyces cerevisiae and Escherichia coli?
abstract: We studied the behavior of the signal recognition particle (SRP) components in Saccharomyces cerevisiae upon deficiencies of the protein transport caused by the absence of the SRP membrane receptor alpha-subunit. A decrease in the concentration of the SRP membrane receptor alpha-subunit in the cell significantly decreased the level of an SRP component, protein SRP72, as well as the levels of mRNAs of SRP protein components and the SRP receptor beta-subunit. But the amount of 7SL RNA remained unchanged. In contrast, in Escherichia coli cells the gradual decrease in the level of the protein FtsY (a homolog of the SRP membrane receptor alpha-subunit) was not associated with changes in the Ffh protein level.
authors.affiliation: Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia. avdeeva@genebee.msu.su
authors.forename: O N
authors.initials: ON
authors.lastname: Kovalskaya
authors.forename: P V
authors.initials: PV
authors.lastname: Sergiev
authors.forename: A A
authors.initials: AA
authors.lastname: Bogdanov
authors.forename: O A
authors.initials: OA
authors.lastname: Dontsova
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D021122:Protein Subunits / Q000378:metabolism; D020733:RNA, Small Cytoplasmic / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics; D018271:Signal Recognition Particle / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: C115990:7SL RNA; D029968:Escherichia coli Proteins; D021122:Protein Subunits; D020733:RNA, Small Cytoplasmic; D029701:Saccharomyces cerevisiae Proteins; D018271:Signal Recognition Particle