title: Characteristics of a mesoporous silicate immobilized trypsin bioreactor in organic media.
abstract: Mesoporous silicates (MPS) materials are attractive materials for immobilizing proteins/enzymes because of their well ordered structures, large surface areas (up to 1000 m(2) g(-1)), narrow pore size distributions, large pore diameters, and pore volumes. MPS with average pore diameters ranging from 28 to 300 Angstroms were prepared using cationic and nonionic surfactants. The influence of water content, pH, storage, and thermal treatment on the activity of trypsin immobilized onto MPS was investigated. In a range of solvents, the amidolytic activity of immobilized trypsin was higher than that of the lyophilized preparation. Significant increases in k(cat)/K(M) occurred in propanol, ethanol, methanol, and formamide of 90, 62, 45 and 26, respectively. The observed increases were primarily a result of substantial increases in k(cat).
authors.affiliation: Materials and Surface Science Institute, Department of Chemical and Environmental Sciences, University of Limerick, Ireland.
authors.forename: Dimple
authors.initials: D
authors.lastname: Goradia
authors.forename: Jakki
authors.initials: J
authors.lastname: Cooney
authors.forename: B Kieran
authors.initials: BK
authors.lastname: Hodnett
authors.forename: Edmond
authors.initials: E
authors.lastname: Magner
mesh_terms: D000438:Alcohols / Q000737:chemistry*; D019149:Bioreactors; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005559:Formamides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010316:Particle Size; D016062:Porosity; D017640:Silicates / Q000737:chemistry*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D014357:Trypsin / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000438:Alcohols; D004800:Enzymes, Immobilized; D005559:Formamides; D017640:Silicates; D013501:Surface-Active Agents; D014867:Water; C031066:formamide; D014357:Trypsin