title: Generation of a conditional null allele of jumonji.
abstract: The jumonji (jmj) gene plays important roles in multiple organ development in mouse, including cardiovascular development. Since JMJ is expressed widely during mouse development, it is essential that conditional knockout approaches be employed to ablate JMJ in a tissue-specific manner to identify the cell lineage specific roles of JMJ. In this report, we describe the establishment of a jmj conditional null allele in mice by generating a loxP-flanked (floxed) jmj allele, which allows the in vivo ablation of jmj via Cre recombinase-mediated deletion. Gene targeting was used to introduce loxP sites flanking exon 3 of the jmj allele to mouse embryonic stem cells. Our results indicate that the jmj floxed allele converts to a null allele in a heart-specific manner when embryos homozygous for the floxed jmj allele and carrying the alpha-myosin heavy chain promoter-Cre transgene were analyzed by Southern and Northern blot analyses. Therefore, this mouse line harboring the conditional jmj null allele will provide a valuable tool for deciphering the tissue and cell lineage specific roles of JMJ.
authors.affiliation: Department of Anatomy, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53706, USA.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Mysliwiec
authors.forename: Junqin
authors.initials: J
authors.lastname: Chen
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Powers
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Bartley
authors.forename: Michael D
authors.initials: MD
authors.lastname: Schneider
authors.forename: Youngsook
authors.initials: Y
authors.lastname: Lee
mesh_terms: D000483:Alleles; D000818:Animals; D019070:Cell Lineage; D005091:Exons; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D005818:Genetic Engineering / Q000379:methods; D006720:Homozygote; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics*; D063151:Polycomb Repressive Complex 2; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013234:Stem Cells / Q000378:metabolism; D019076:Transgenes
chemical_list: C093650:Jarid2 protein, mouse; D009419:Nerve Tissue Proteins; D063151:Polycomb Repressive Complex 2; C045073:Cre recombinase; D019426:Integrases