PACAP and ceramides exert opposite effects on migration, neurite outgrowth, and cytoskeleton remodeling.
During brain development, cells that fail to reach their final destination or to establish proper connections are eliminated. It has been shown that the proinflammatory cytokine second messenger ceramides and the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) play pivotal roles in the histogenesis of the cerebellum. However, little is known regarding the effects of these two factors on cerebellar granule cell migration. We have found that PACAP prevents the effects of C2-ceramide on granule cell motility and neurite outgrowth. These actions are attributable to opposite effects on actin distribution, tubulin polymerization, and Tau phosphorylation. These data suggest that PACAP and factors inducing ceramide production may control granule cell migration during cerebellar development.
Falluel-Morel
Anthony
A
INSERM U413, IFRMP 23, University of Rouen, 76821 Mont-Saint-Aignan, France.
Vaudry
David
D
Aubert
Nicolas
N
Galas
Ludovic
L
Benard
Magalie
M
Basille
Magali
M
Fontaine
Marc
M
Fournier
Alain
A
Vaudry
Hubert
H
Gonzalez
Bruno J
BJ
D017209:Apoptosis / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D002518:Ceramides / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000494:pharmacology*
D002518:Ceramides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide