Identification of transmembrane domain of a membrane associated protein NS5 of Dendrolimus punctatus cytoplasmic polyhedrosis virus.
We examined the intracellular localization of NS5 protein of Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV) by expressing NS5-GFP fusion protein and proteins from deletion mutants of NS5 in baculovirus recombinant infected insect Spodoptera frugiperda (Sf-9) cells. It was found that the NS5 protein was present at the plasma membrane of the cells, and that the N-terminal portion of the protein played a key role in the localization. A transmembrane region was identified to be present in the N-terminal portion of the protein, and the detailed transmembrane domain (SQIHMVWVKSGLVFF, 57-71aa) of N-terminal portion of NS5 was further determined, which was accorded with the predicted results, these findings suggested that NS5 might have an important function in viral life cycle.
Chen
Wuguo
W
State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, P. R. China.
Zhang
Jiamin
J
Dong
Changjin
C
Yang
Bo
B
Li
Yanqiu
Y
Liu
Chuanfeng
C
Hu
Yuanyang
Y
D000818:Animals; D002460:Cell Line; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins; D007304:Insect Viruses / Q000737:chemistry*; D016253:Microscopy, Immunoelectron; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry; D012087:Reoviridae / Q000737:chemistry*; D018411:Spodoptera / Q000166:cytology; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000737:chemistry*
D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; D049452:Green Fluorescent Proteins