title: Differential expression of the detoxification genes in the different life stages of the malaria vector Anopheles gambiae.
abstract: The diverse habitats and diets encountered during the life cycle of an Anopheles mosquito have necessitated the development of extensive families of detoxification enzymes. Expansion of the three detoxification enzyme families (cytochrome P450s, carboxylesterases and glutathione transfereases), has occurred in mosquitoes compared with Drosophila, however, very little is known regarding the developmental expression of theses genes. Using a custom made microarray we determined the expression profile of the detoxification genes in adults, larvae and pupae of the malaria vector A. gambiae. The expression of approximately one quarter of these genes was developmentally regulated. The expression profile of each of these genes and the information this data provides on putative functions of the mosquito detoxification enzymes is discussed.
authors.affiliation: Vector Research Group, Liverpool School of Tropical Medicine, Liverpool, UK. C.Strode@liv.ac.uk
authors.forename: C
authors.initials: C
authors.lastname: Strode
authors.forename: K
authors.initials: K
authors.lastname: Steen
authors.forename: F
authors.initials: F
authors.lastname: Ortelli
authors.forename: H
authors.initials: H
authors.lastname: Ranson
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism*; D007303:Insect Vectors / Q000235:genetics* / Q000378:metabolism; D008018:Life Cycle Stages / Q000235:genetics / Q000502:physiology*; D046228:Microarray Analysis
chemical_list: D019476:Insect Proteins; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D002265:Carboxylic Ester Hydrolases