Protein synthesized by dengue infected Aedes aegypti and Aedes albopictus.
The main objective of this study was to compare protein profiles of whole mosquitoes of Malaysian Aedes aegypti and Aedes albopictus after infection with virus and to investigate whether dengue virus would induce protein secretion in Ae. aegypti and Ae. albopictus. Using SDS -PAGE, it was shown that in uninfected Ae. aegypti and Ae. albopictus, the protein bands were within the range of 14 - 80 kDa with most of the bands overlapping for the two species. Comparison of the protein profile of infected and uninfected Ae. aegypti and Ae. albopictus showed five distinct molecular weight grouping at 73 - 76 kDa (Group 1), 44 - 50 kDa (Group 2), 28 - 31 kDa (Group 3), 20 - 25 kDa (Group 4) and 14 - 17 kDa (Group 5). Predominant bands for both species (infected and uninfected) were between 21 - 25 kDa and 44 - 50 kDa. Protein bands having a molecular weight of 70 kDa were only present in infected Ae. albopictus and those bands having molecular weight of 21 kDa were observed only in infected Ae. aegypti. The rate of digestion of blood meals was more rapid in Ae. albopictus than Ae. aegypti. Uninfected Ae. albopictus completed the blood digestion 2 days after ingestion of a blood meal whereas Ae. aegypti needed 3 days to complete the digestion. The rate of digestion for blood meals was slower for both mosquito species when fed with dengue virus infected blood. The digestion processes were completed 3 and 4 days after blood ingestion for Ae. albopictus and Ae. aegypti, respectively. This could be due to the presence of dengue virus in the blood, which slow down the digestion process. Appearance and disappearance of new protein bands was also observed even after the digestion has completed for both infected mosquito species. In conclusion, dengue virus was shown to induce specific proteins in both Ae. aegypti and Ae. albopictus.
Rohani
A
A
Unit of Medical Entomology, Infectious Diseases Research Centre, Institute for Medical Research, Kuala Lumpur.
Yunus
W
W
Zamree
I
I
Lee
H L
HL
D000330:Aedes / Q000145:classification / Q000502:physiology / Q000821:virology*; D000818:Animals; D001522:Behavior, Animal; D003716:Dengue Virus / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005247:Feeding Behavior; D005260:Female; D054884:Host-Pathogen Interactions; D019476:Insect Proteins / Q000378:metabolism*; D007303:Insect Vectors / Q000821:virology*
D019476:Insect Proteins