Methods for identifying and mapping recent segmental and gene duplications in eukaryotic genomes.
The aim of this chapter is to provide instruction for analyzing and mapping recent segmental and gene duplications in eukaryotic genomes. We describe a bioinformatics-based approach utilizing computational tools to manage eukaryotic genome sequences to characterize and understand the evolutionary fates and trajectories of duplicated genes. An introduction to bioinformatics tools and programs such as BLAST, Perl, BioPerl, and the GFF specification provides the necessary background to complete this analysis for any eukaryotic genome of interest.
Khaja
Razi
R
Program in Genetics and Genomic Biology, Research Institute, The Hospital for Sick Children, Toronto, ON, Canada.
MacDonald
Jeffrey R
JR
Zhang
Junjun
J
Scherer
Stephen W
SW
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D019295:Computational Biology; D030541:Databases, Genetic; D020440:Gene Duplication; D023281:Genomics; D006801:Humans; D051379:Mice; D016415:Sequence Alignment; D012984:Software