Detection of DNA using cationic polyhedral oligomeric silsesquioxane nanoparticles as the probe by resonance light scattering technique.
A novel cationic polyhedral oligomeric silsesquioxane nanoparticle (cationic POSS) was synthesized and successfully used as a new probe for the detection of DNA by resonance light scattering technique (RLS). It was found that the electrostatic interaction of cationic POSS and DNA could obviously enhance the RLS signal, the enhanced RLS intensity at 360 nm was proportional to the concentration of nucleic acids within the range of 0.35-42.82 microg ml-1 for calf thymus DNA, the determination limit (3sigma) was 0.32 ng ml-1. The results showed this method was very sensitive, convenient, rapid and reproducible.
Zou
Qi-Chao
QC
Faculty of Chemistry and Material Science, Hubei University, Wuhan 430062, PR China.
Yan
Qiu-Jun
QJ
Song
Gong-Wu
GW
Zhang
Shi-Lin
SL
Wu
Li-Min
LM
D004247:DNA / Q000032:analysis*; D008027:Light; D053758:Nanoparticles; D017646:Organosilicon Compounds; D012542:Scattering, Radiation
C512088:1,3-bis(1,1,3,3,5,5-hexaethoxy-1,3,5-trisilacyclohexyl)propane; D017646:Organosilicon Compounds; D004247:DNA