MASQOT-GUI: spot quality assessment for the two-channel microarray platform.
MASQOT-GUI provides an open-source, platform-independent software pipeline for two-channel microarray spot quality control. This includes gridding, segmentation, quantification, quality assessment and data visualization. It hosts a set of independent applications, with interactions between the tools as well as import and export support for external software. The implementation of automated multivariate quality control assessment, which is a unique feature of MASQOT-GUI, is based on the previously documented and evaluated MASQOT methodology. Further abilities of the application are outlined and illustrated.


BACKGROUND
MASQOT-GUI is Java-based and licensed under the GNU LGPL. Source code and installation files are available for download at http://masqot-gui.sourceforge.net/
Bylesjö
Max
M
Research group for Chemometrics, Department of Chemistry, Umeå University SE-901 87 Umeå, Sweden. max.bylesjo@chem.umu.se
Sjödin
Andreas
A
Eriksson
Daniel
D
Antti
Henrik
H
Moritz
Thomas
T
Jansson
Stefan
S
Trygg
Johan
J
D003196:Computer Graphics; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods / Q000592:standards; D016247:Information Storage and Retrieval / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D011786:Quality Control; D012984:Software; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D014584:User-Computer Interface