title: Human and bovine lactoferrins in the milk of recombinant human lactoferrin-transgenic dairy cows during lactation.
abstract: Seven Friesian human lactoferrin (hLf)-transgenic primiparous dairy cows expressing recombinant hLf (rhLf) in their milk were included in the study. After calving, concentrations of rhLf and bovine LF (bLf) in the milk, somatic cell count and milk yield were determined. The concentration of rhLf was found to be constant, about 2.9 mg/mL, throughout the early lactation period of 3 months. The concentration of bLf in colostrum was higher after calving, but decreased rapidly during the first days of lactation. The mean concentration of bLf was 0.15 mg/mL, but concentrations varied between cows from 0.07 mg/mL to 0.26 mg/mL. Based on that, it may be possible to improve the non-specific host defence mechanism in the mammary gland of dairy cows by enhancing the content of rhLf in the milk.
authors.affiliation: Institute of Applied Biotechnology, University of Kuopio, Kuopio, Finland. paula.hyvonen@uku.fi
authors.forename: Paula
authors.initials: P
authors.lastname: Hyvönen
authors.forename: Leena
authors.initials: L
authors.lastname: Suojala
authors.forename: Johanna
authors.initials: J
authors.lastname: Haaranen
authors.forename: Atte
authors.initials: A
authors.lastname: von Wright
authors.forename: Satu
authors.initials: S
authors.lastname: Pyörälä
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D002417:Cattle / Q000235:genetics* / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D006801:Humans; D007774:Lactation / Q000378:metabolism*; D007781:Lactoferrin / Q000235:genetics / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D007781:Lactoferrin