Involvement of protein kinase C in the PACAP-induced differentiation of neural stem cells into astrocytes.
Expression of members of the conventional protein kinase C (cPKC) family in the differentiation of mouse neural stem cells (NSCs) induced by pituitary adenylate cyclase-activating polypeptide (PACAP) was investigated. In particular, expression of the alpha and beta subtypes of cPKC in NSCs was observed. In response to activation by PACAP, cPKCbeta transiently increased twofold by day 2 and returned to basal levels by day 4, suggesting that cPKCbeta might be responsible for the differentiation process.
Watanabe
Jun
J
Laboratory of Biochemistry, Yokohama College of Pharmacy, 601 Matanocho, Totsuka-Ku, Yokohama, Kanagawa 245-0066, Japan. s.nakajou@hamayaku.ac.jp.
Ohno
Fusako
F
Shioda
Seiji
S
Kikuyama
Sakae
S
Nakaya
Kazuyasu
K
Nakajo
Shigeo
S
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000494:pharmacology*; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
D007527:Isoenzymes; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011493:Protein Kinase C