Effects of wogonin on the levels of cytokines and functions of leukocytes associated with NF-kappa B expression in Sprague-Dawley rats.
The objectives of the study were to investigate the effects of wogonin on the cytotoxicity, natural killer activity, macrophage phagocytosis, release of interleukin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha), the expression of nuclear factor-kappaB (NF-kappaB) in leukocytes from Sprague-Dawley rats. Wogonin was added to the medium and the levels of IL-1alpha and TNF-alpha in the medium were measured using enzyme-linked immunosorbent assay. Both IL-1alpha and TNF-alpha mRNAs were measured by reverse transcription polymerase chain reaction and the levels of NF-kappaB and I-kappaB were measured by Western blotting. Natural killer cell activity and macrophage phagocytosis were measured by flow cytometry. The results demonstrated that wogonin induced cytotoxicity in rat leukocytes and promoted the activities of natural killer cells and macrophages. Wogonin increased the IL-1alpha and TNF-alpha levels in the leukocytes in culture media dose-dependently. IL-1alpha and TNF-alpha mRNA elevations were also promoted by wogonin. The data suggests that wogonin may affect the IL-1alpha and TNF-alpha mRNA expressions by affecting the NF-kappaB in rat leukocytes.
Tang
Nou-Ying
NY
School of Chinese Medicine, China Medical University Hospital, Taichung City, Taiwan.
Yang
Jai-Sing
JS
Chang
Yung-Hsien
YH
Lu
Hsu-Feng
HF
Hsia
Te-Chun
TC
Lin
Wen-Chuan
WC
Chung
Jing-Gung
JG
D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D044950:Flavanones / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D020672:I-kappa B Proteins / Q000032:analysis / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D010587:Phagocytosis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism
D016207:Cytokines; D044950:Flavanones; D005419:Flavonoids; D020672:I-kappa B Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C085514:wogonin