Rapid and high-throughput forensic short tandem repeat typing using a 96-lane microfabricated capillary array electrophoresis microdevice.
A 96-channel microfabricated capillary array electrophoresis (muCAE) device was evaluated for forensic short tandem repeat (STR) typing using PowerPlex 16 and AmpFlSTR Profiler Plus multiplex PCR systems. The high-throughput muCAE system produced high-speed <30-min parallel sample separations with single-base resolution. Forty-eight previously analyzed single-source samples were accurately typed, as confirmed on an ABI Prism 310 and/or the Hitachi FMBIO II. Minor alleles in 3:1 mixture samples containing female and male DNA were reliably typed as well. The instrument produced full profiles from sample DNA down to 0.17 ng, a threshold similar to that found for the ABI 310. Seventeen nonprobative samples from various evidentiary biological stains were also correctly typed. The successful application of the muCAE device to actual forensic STR typing samples is a significant step toward the development of a completely integrated STR analysis microdevice.
Yeung
Stephanie H I
SH
UCSF/UCB Joint Graduate Group in Bioengineering, University of California, Berkeley, CA 94720, USA.
Greenspoon
Susan A
SA
McGuckian
Amy
A
Crouse
Cecelia A
CA
Emrich
Charles A
CA
Ban
Jeffrey
J
Mathies
Richard A
RA
D000483:Alleles; D016172:DNA Fingerprinting / Q000295:instrumentation* / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008904:Miniaturization; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
D005819:Genetic Markers