title: Proteomic analysis of Escherichia coli biofilms reveals the overexpression of the outer membrane protein OmpA.
abstract: Bacterial colonisation and biofilm formation on the surface of urinary catheters is a common cause of nosocomial infection, and as such is a major impediment to their long-term use. Understanding the mechanisms of biofilm formation on urinary catheters is critical to their control and will aid the future development of materials used in their manufacture. In this report we have used proteomic analysis coupled with immunoassays to show that the major outer membrane protein (OmpA) of Escherichia coli is overexpressed during biofilm formation. A series of synthetic hydrogels being developed for potential use as catheter coatings were used as the substrata and OmpA expression was increased in biofilms on all these surfaces, as well as being a feature of both a laboratory and a clinical strain of E. coli. Up-regulation of OmpA may, therefore, be a common feature of E. coli biofilms. These findings present OmpA as a potential target for biofilm inhibition and may contribute to the rational design of biofilm inhibiting hydrogel coatings for urinary catheters.
authors.affiliation: University of Manchester, Faculty of Medicine and Human Health, Centre for Molecular Medicine, Department of Medical Genetics, Manchester, UK.
authors.forename: Rowan
authors.initials: R
authors.lastname: Orme
authors.forename: C W Ian
authors.initials: CW
authors.lastname: Douglas
authors.forename: Stephen
authors.initials: S
authors.lastname: Rimmer
authors.forename: Michelle
authors.initials: M
authors.lastname: Webb
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D020100:Hydrogels / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D008689:Methacrylates / Q000737:chemistry; D015394:Molecular Structure; D040901:Proteomics / Q000379:methods*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D020100:Hydrogels; D008689:Methacrylates; C078167:OMPA outer membrane proteins