title: IL-10 is up regulated in early and transitional stages in vervet monkeys experimentally infected with Trypanosoma brucei rhodesiense.
abstract: IL-10 has been suggested as a possible parameter for human African trypanosomiasis stage determination. However, conclusive experimental studies have not been carried out to evaluate this, which is a prerequisite before a potential test can be validated in humans for diagnostic purposes. We used the vervet monkey model of trypanosomiasis to scrutinize IL-10 in blood and cerebrospinal fluid (CSF). Five adult males were experimentally infected with T. b. rhodesiense. The infected animals became anemic and exhibited weight loss. Parasitemia was patent after 3 days and fluctuated around 3.7 x 10(7) trypanosomes/ml throughout the experimental period. The total CSF white cell counts increased from pre-infection means around 3 cells/micro l to a peak of 30 cells/micro l, 42 days post-infection (DPI). IL-10 was not detectable (<2 pg/ml) in serum prior to infection. IL-10 serum concentrations increased to 273 pg/ml 10 DPI coinciding with the first peak of parasitemia. Thereafter the levels declined to a mean value of 77 pg/ml 34 DPI followed by a significant rise to a second peak of 304 pg/ml (p<0.008) 42 DPI. There was no detectable IL-10 in CSF. IL-10 synthesis is thus stimulated both in the early and transitional stages of experimental trypanosomiasis. That IL-10 is produced in early stage disease is an interesting finding unlikely to be detected in humans where it is difficult to determine the exact time of infection. The IL-10 peak observed on day 42 of infection might indicate onset of parasite neuroinvasion coinciding with a peak in white blood cell counts in the blood and CSF.
authors.affiliation: KARI-Trypanosomiasis Research Centre, Kikuyu, Kenya.
authors.forename: Maina
authors.initials: M
authors.lastname: Ngotho
authors.forename: Naomi
authors.initials: N
authors.lastname: Maina
authors.forename: John
authors.initials: J
authors.lastname: Kagira
authors.forename: Felix
authors.initials: F
authors.lastname: Royo
authors.forename: Idle O
authors.initials: IO
authors.lastname: Farah
authors.forename: Jann
authors.initials: J
authors.lastname: Hau
mesh_terms: D000818:Animals; D001835:Body Weight; D002555:Cerebrospinal Fluid / Q000166:cytology; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D006400:Hematocrit; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000502:physiology; D007958:Leukocyte Count; D008297:Male; D009206:Myocardium / Q000473:pathology; D013997:Time Factors; D016833:Trypanosoma brucei rhodesiense / Q000472:pathogenicity*; D014353:Trypanosomiasis, African / Q000097:blood / Q000276:immunology / Q000503:physiopathology*; D015854:Up-Regulation / Q000276:immunology
chemical_list: D016753:Interleukin-10