title: [Prevalence of Borrelia burgdorferi sensu lato in ticks, Ixodes ricinus in Tarnowskie Góry district].
abstract: Borrelia burgdorferi is an aetiological factor of borreliosis (Lyme disease). The main vectors of Borrelia burgdorferi are larvae, nymphs, and females of Ixodes ricinus. The aim of this paper was to analyse infection parameters of Borrelia burgdorferi in a selected populations of Ixodes ricinus. The study was conducted in Tarnowskie Góry administrative district (Krupski Młyn, Zielona, Lubliniec, Tarnowskie Góry, Swierklaniec, Tworóg, and Zbrosławice). A total of 85 ticks were collected with a piece of cloth dragged over the vegetation. The Borrelia burgdorferi infection was confirmed with a PCR method, using flagellin protein gene DNA amplification. DNA of Borrelia burgderferi sensu lato was present in 14 ticks, which constituted 16.5% of the population studied. The percentage of infected females and nymphs was 26.8%, 22.2%, and 5.6%, respectively. A high prevalence of the pathogen (50%) was in ticks revealed in the recreation areas and the community forest in Swierklaniec.
authors.affiliation: Katedra i Zakład Ogólnej Biologii Lekarskiej, Slaska Akademia Medyczna, Zabrze.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Spausta
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wiczkowski
authors.forename: Jolanta
authors.initials: J
authors.lastname: Ciarkowska
authors.forename: Joanna
authors.initials: J
authors.lastname: Strzelczyk
authors.forename: Gizela
authors.initials: G
authors.lastname: Trapp
authors.forename: Brygida
authors.initials: B
authors.lastname: Adamek
authors.forename: Marzena
authors.initials: M
authors.lastname: Zalewska-Ziob
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000235:genetics / Q000382:microbiology*; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D018884:Ixodes / Q000235:genetics / Q000382:microbiology*; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013045:Species Specificity; D013984:Tick Infestations / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial