[Effects and significance of gas explosion on expression of NF-kappaB and ICAM1 in lung tissue of rats].
OBJECTIVE
To explore the expression of NF-kappaB and ICAM-1 in the gas explosion wounded lung of rats and the relationship.


METHODS
Digoxin labeled NF-kappaB was used as probe. In situ hybridization was performed to detect the NF-kappaB mRNA. Immunohistochemistry was used to detect the expression of NF-kappaB and ICAM-1.


RESULTS
The levels of NF-kappaB mRNA, the expression of NF-kappaB and ICAM-1 in the wounded rats were significantly increased and reached their peak two hours after injury. Pathology of lung tissue showed that some crachea epithelium mucosae were desquamated; congestion, edema of trachea wall and infiltration of neutrophilic granulocytes were found; hemorrhage, edema and infiltration of lots of inflammatory cells were present in alveolus cells. Electron microscope showed that type I, especially type II alveolus epithelia had degeneration and desquamation.


CONCLUSIONS
The injury of gas explosion can activate NF-kappaB, which has close correlation with the acute injury to lung.
Liu
Lin-hong
LH
General Hospital of Coal Mining Group of Pingdingshan, Henan Province 467000, China.
Ren
Wen-jie
WJ
Wang
Gui-zhen
GZ
Qin
Zu-xuan
ZX
Wang
Xiao-min
XM
Wu
Song-yuan
SY
Liu
Ya
Y
Liu
Liang-ming
LM
D000818:Animals; D001753:Blast Injuries / Q000378:metabolism* / Q000473:pathology; D005107:Explosions; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D016328:NF-kappa B; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1