Evaluation of oriental medicinal herbs for estrogenic and antiproliferative activities.
Herb extracts commercially used in Asia were screened for their estrogenic activity with a recombinant yeast system with both a human estrogen receptor (ER) expression plasmid and a reporter plasmid. Pueraria lobata (flower) had the highest estrogenic relative potency (RP, 17-estradiol = 1.00) (7.8e-3) (RP for + control), followed by Amomum xanthioides (1.3e-3), Glycyrrhiza uralensis, Zingiber officinale, Rheum palmatum, Curcuma aromatica, Eriobotrya japonica, Sophora flavescens, Anemarrhena asphodeloides, Polygonum multiflorum and Pueraria lobata (root) (9.5e-4-1.0e-4), and Prunus persica, Lycoppus lucidus and Adenophora stricta (9.0e-5-8.0e-5). In the antiproliferative assay, five human cancer cell lines representing different tissues (breast, lung and ovary) were used. Eriobotrya japonica showed strong cytotoxicity in ER-negative breast cancer (MDA-MB-231), cervix epitheloid (HeLa) and lung (A549) carcinoma cell lines.
Kang
Se Chan
SC
College of Pharmacy, SungKyunKwan University, Suwon, Gyonggi-Do, South Korea.
Lee
Chang Min
CM
Choi
Han
H
Lee
Jae Hyun
JH
Oh
Joa Sub
JS
Kwak
Jong Hwan
JH
Zee
Ok Pyo
OP
D001208:Asia; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004967:Estrogens / Q000633:toxicity*; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D000432:Methanol / Q000737:chemistry; D030781:Organisms, Genetically Modified / Q000502:physiology; D010936:Plant Extracts / Q000633:toxicity*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000633:toxicity*; D011960:Receptors, Estrogen / Q000187:drug effects; D012441:Saccharomyces cerevisiae; D018675:Toxicity Tests / Q000379:methods; D001616:beta-Galactosidase / Q000032:analysis / Q000378:metabolism
D004967:Estrogens; D010936:Plant Extracts; D011960:Receptors, Estrogen; D001616:beta-Galactosidase; D000432:Methanol