title: Secretion of RANTES (CCL5) and interleukin-10 from mesenteric adipose tissue and from creeping fat in Crohn's disease: regulation by steroid treatment.
abstract: BACKGROUND
  Creeping fat represents a characteristic feature of Crohn's disease (CD) and adipose tissue is currently being recognized as a complex compartment secreting highly active molecules. Pro- or anti-inflammatory adipose tissue-derived secretory products (adipocytokines) might play a role in the pathogenesis of CD.
  
  
  METHODS
  Adipose tissue specimens were obtained from creeping fat contiguous to the involved intestine of 10 patients with CD. Mesenteric adipose tissue specimens resected in 13 patients with colon cancer (CC) and in seven patients with diverticulitis (DIV) served as controls. Three fat tissue specimens per well and n = 6-8 wells per patient were incubated ex vivo for 24 h. The release of regulated on activation, T-cell expressed and secreted (RANTES) and interleukin (IL)-10 into the supernatant was measured by ELISA.
  
  
  RESULTS
  Both RANTES and IL-10 secretion could be demonstrated from total adipose tissue explants. The RANTES secretion is increased from creeping fat in CD (3691 +/- 597 pg/g fat per 24 h) when compared to mesenteric adipose tissue from patients with CC (1690 +/- 191 pg/g fat per 24 h; P < 0.0001) or DIV (1672 +/- 336 pg/g fat per 24 h; P < 0.0001). In contrast, IL-10 secretion is downregulated significantly only in patients with DIV (1418 +/- 180 pg/g fat per 24 h; P = 0.016) when compared to CC patients (2368 +/- 259 pg/g fat per 24 h). Crohn's disease patients receiving steroids had a higher secretion rate of RANTES and IL-10.
  
  
  CONCLUSIONS
  Both RANTES and IL-10 secretion can be detected from mesenteric adipose tissue and from creeping fat. The elevated RANTES and IL-10 secretion from creeping fat in CD is not due to a CD-specific effect but caused by steroid treatment.
authors.affiliation: Department of Internal Medicine I, University of Regensburg, Regensburg, Germany. andreas.schaeffler@klinik.uni-regensburg.de
authors.forename: Andreas
authors.initials: A
authors.lastname: Schäffler
authors.forename: Alois
authors.initials: A
authors.lastname: Fürst
authors.forename: Christa
authors.initials: C
authors.lastname: Büchler
authors.forename: Gisela
authors.initials: G
authors.lastname: Paul
authors.forename: Gerhard
authors.initials: G
authors.lastname: Rogler
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schölmerich
authors.forename: Hans
authors.initials: H
authors.lastname: Herfarth
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D002097:C-Reactive Protein / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism*; D018925:Chemokines / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism; D003424:Crohn Disease / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D016207:Cytokines / Q000378:metabolism; D004238:Diverticulitis / Q000378:metabolism; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D008297:Male; D008643:Mesentery / Q000378:metabolism; D008875:Middle Aged; D013256:Steroids / Q000627:therapeutic use*
chemical_list: D018946:Chemokine CCL5; D018925:Chemokines; D016207:Cytokines; D013256:Steroids; D016753:Interleukin-10; D002097:C-Reactive Protein