title: Ectopic expression of N-acetylglucosamine 6-O-sulfotransferase 2 in chemotherapy-resistant ovarian adenocarcinomas.
abstract: Mucinous and clear cell adenocarcinomas are the major histological types of ovarian epithelial cancer and are associated with a poor prognosis due to their resistance to chemotherapy. A novel tumor marker specific for ovarian mucinous and clear cell adenocarcinomas would be helpful for overcoming these serious diseases. We showed previously by enzymological characterization and RT-PCR that colonic mucinous adenocarcinoma tissues ectopically express GlcNAc6ST-2, a member of the carbohydrate 6-O-sulfotransferase family (Seko, A. et al. (2002) Glycobiology 12, 379-388). Here, we prepared a GlcNAc6ST-2-specific polyclonal antibody for immunohistochemical analysis and found that GlcNAc6ST-2 is ectopically expressed by not only colonic mucinous adenocarcinomas but also ovarian mucinous, clear cell and papillary serous adenocarcinomas. In contrast, solid serous adenocarcinomas, endometrioid adenocarcinomas, and mucinous adenomas expressed GlcNAc6ST-2 much less frequently or not at all. RT-PCR analysis confirmed that GlcNAc6ST-2 transcripts are expressed in ovarian mucinous adenocarcinomas but not in mucinous adenomas. In addition, immunohistochemical analysis using sulfated glycan-specific monoclonal antibodies showed that ovarian adenocarcinoma cells express GlcNAc 6-O-sulfated glycans, including an L-selectin ligand and its related glycans. These results indicate that GlcNAc6ST-2 would be a novel tumor antigen that is specifically expressed in ovarian mucinous, clear cell and papillary serous adenocarcinomas.
authors.affiliation: Hanno Discovery Center, TAIHCO Pharmaceutical Co. Ltd., Saitama, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kanoh
authors.forename: Akira
authors.initials: A
authors.lastname: Seko
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ideo
authors.forename: Midori
authors.initials: M
authors.lastname: Yoshida
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Nomoto
authors.forename: Suguru
authors.initials: S
authors.lastname: Yonezawa
authors.forename: Masaru
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Reiji
authors.initials: R
authors.lastname: Kannagi
authors.forename: Katsuko
authors.initials: K
authors.lastname: Yamashita
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D015238:Sulfotransferases / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured; D000097811:Carbohydrate Sulfotransferases
chemical_list: D000951:Antigens, Neoplasm; D015238:Sulfotransferases; D000097811:Carbohydrate Sulfotransferases