[Duration of tau hyperphosphorylation and spatial memory deficit induced by single injection of Forskolin into lateral ventricle of rat].
OBJECTIVE
To investigate the duration of tau hyperphosphorylation and spatial memory retentive deficit induced by single injecting with Forskolin, a protein kinase A activator, into lateral ventricle of rats, and the correlation between the two pathological alterations.


METHODS
Forskolin (80 micromol/L, 40 microl) was injected into the lateral ventricle by stereotaxic injection. Tau phosphorylation and spatial memory retention were measured by Western blot/immunocytochemistry and Morris-Water-Maze test, respectively.


RESULTS
The phosphorylation levels of tau at Tau-1, PHF-1, and pS214 epitopes were significantly elevated at 24, 48 and 72 h after single administration of Forskolin (P < 0.05). The most significant elevation was seen at 48 h (P < 0.01) and it tended to recover at 72 h (P < 0.05) after injection. The correlation between the two pathological alterations was positive at PHF-1 site (r = 0.97, P < 0.05), negative at Tau-1 site (r = -0.963, P < 0.05), and not significant at pS214 site (r = 0.705, P > 0.05).


CONCLUSIONS
Forskolin can induce tau hyperphosphorylation and spatial memory retentive deficit within a certain period of time. The level of tau phosphorylation in hippocampus is somehow correlated with the spatial memory deficit in rats.
Zhang
Jun-Xia
JX
Department of Pathophysiology, Tongji Medical College, Huazhong Science and Technical University, Wuhan 430030, China.
Wang
Yue
Y
Tian
Qing
Q
Zhu
Ling-Qiang
LQ
Wang
Jian-Zhi
JZ
D000818:Animals; D005576:Colforsin / Q000494:pharmacology*; D007276:Injections, Intraventricular; D020547:Lateral Ventricles; D008297:Male; D008569:Memory Disorders / Q000139:chemically induced*; D010766:Phosphorylation; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D016875:tau Proteins / Q000378:metabolism*
D016875:tau Proteins; D005576:Colforsin