Two GacA-dependent small RNAs modulate the quorum-sensing response in Pseudomonas aeruginosa.
In Pseudomonas aeruginosa, the GacS/GacA two-component system positively controls the quorum-sensing machinery and the expression of extracellular products via two small regulatory RNAs, RsmY and RsmZ. An rsmY rsmZ double mutant and a gacA mutant were similarly impaired in the synthesis of the quorum-sensing signal N-butanoyl-homoserine lactone, the disulfide bond-forming enzyme DsbA, and the exoproducts hydrogen cyanide, pyocyanin, elastase, chitinase (ChiC), and chitin-binding protein (CbpD). Both mutants showed increased swarming ability, azurin release, and early biofilm development.
Kay
Elisabeth
E
Département de Microbiologie Fondamentale, Université de Lausanne, Building Biophore, CH-1015 Lausanne, Switzerland.
Humair
Bérénice
B
Dénervaud
Valérie
V
Riedel
Kathrin
K
Spahr
Stéphanie
S
Eberl
Leo
L
Valverde
Claudio
C
Haas
Dieter
D
D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism
D001426:Bacterial Proteins; C091100:GacA protein, Bacteria; D012329:RNA, Bacterial; D012269:Ribosomal Proteins