[Fusion tags technology and their applications].
Fusion tags are originally developed to facilitate the purification of recombinant protein from crude extracts. In recent years, the discovery of different tags and the development of fusion strategy make the function of fusion tags diversified. However, there was no a cure-all fusion tag for different applications. We here give an overview of fusion tag technology and the different applications of fusion tags, including the purification, detection and oriented immobilization of recombinant protein, the visualization of bioevent in vivo, the enhancement of the yield of protein, the improvement of the solubility and stability of the expressed protein.
Li
Yong-Jin
YJ
Protein Research Platform, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Chen
Yuan-Yuan
YY
Bi
Li-Jun
LJ
D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012995:Solubility
D011994:Recombinant Proteins