title: A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor I7.
abstract: In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor I7 (OR I7) in its membrane fraction. The OR I7 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy (EIS) measurements on samples and controls.
authors.affiliation: Centre de Génie Electrique de Lyon (CEGELY), Ecole Centrale de Lyon, 69134 Ecully Cedex, France. yanxiahou24@yahoo.com
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Hou
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
authors.forename: Claude
authors.initials: C
authors.lastname: Martelet
authors.forename: Aidong
authors.initials: A
authors.lastname: Zhang
authors.forename: Jasmina
authors.initials: J
authors.lastname: Minic-Vidic
authors.forename: Tatiana
authors.initials: T
authors.lastname: Gorojankina
authors.forename: Marie-Annick
authors.initials: MA
authors.lastname: Persuy
authors.forename: Edith
authors.initials: E
authors.lastname: Pajot-Augy
authors.forename: Roland
authors.initials: R
authors.lastname: Salesse
authors.forename: Vladimir
authors.initials: V
authors.lastname: Akimov
authors.forename: Lino
authors.initials: L
authors.lastname: Reggiani
authors.forename: Cecilia
authors.initials: C
authors.lastname: Pennetta
authors.forename: Eleonora
authors.initials: E
authors.lastname: Alfinito
authors.forename: Oscar
authors.initials: O
authors.lastname: Ruiz
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gomila
authors.forename: Josep
authors.initials: J
authors.lastname: Samitier
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Errachid
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D017097:Electric Impedance; D009812:Odorants / Q000032:analysis*; D015202:Protein Engineering; D051381:Rats; D018035:Receptors, Odorant; D013057:Spectrum Analysis
chemical_list: D018035:Receptors, Odorant; C509506:olfactory receptor I7, rat