Hydrogen peroxide-induced astaxanthin biosynthesis and catalase activity in Xanthophyllomyces dendrorhous.
Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) in shake-flask cultures was exposed to 10-20 mmol/L H(2)O(2) at various culture stages, and the astaxanthin production was significantly increased by H(2)O(2) fed at 0 or 24 h (exponential phase), but only slightly at 48 h (near stationary phase). The astaxanthin production was enhanced most significantly with double feeding of 10 mmol/L H(2)O(2) at 0 and 24 h, reaching a cellular content of 1.30 mg/g cell and a volumetric yield of 10.4 mg/L, which were 83 and 65% higher, respectively, than those of the control (0.71 mg/g cell and 6.3 mg/L). The intracellular catalase (CAT) activity was also increased after H(2)O(2) treatment. The increases in CAT and astaxanthin of cells could be detected within 4 h of H(2)O(2) treatment. The increase in the astaxanthin content of cells was concomitant with a notable decrease in the beta-carotene content. The older yeast cells at late culture stage (120 h), due perhaps in part to their higher astaxanthin contents, were more tolerant to H(2)O(2) toxicity than the younger cells (24 h). No enhancement of the astaxanthin biosynthesis was attained when H(2)O(2) was added to the yeast culture together with a sufficient amount of exogenous CAT. The results suggest that astaxanthin biosynthesis in X. dendrorhous can be stimulated by H(2)O(2) as an antioxidative response.
Liu
Yuan Shuai
YS
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Wu
Jian Yong
JY
D001487:Basidiomycota / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D004271:DNA, Fungal; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018384:Oxidative Stress; D024341:Xanthophylls / Q000096:biosynthesis; D019207:beta Carotene / Q000737:chemistry / Q000378:metabolism
D004271:DNA, Fungal; D024341:Xanthophylls; D019207:beta Carotene; C005948:astaxanthine; D006861:Hydrogen Peroxide; D002374:Catalase