title: Expression of CYLD, NF-kappaB and NF-kappaB-related factors in salivary gland tumors.
abstract: The cylindromatosis (CYLD) gene was originally identified as a tumor suppressor that is mutated in familial cylindromatosis, an autosomal dominant condition that confers a predisposition to multiple tumors of the skin appendages. CYLD has deubiquitinating enzyme activity and inhibits the activation of transcription factor NF-kappaB. Therefore, loss of CYLD function correlates with tumorigenesis. Expression of CYLD has been detected in various organs, but its expression in salivary gland tumor (SGT) is still unknown. Adenoid cystic carcinoma (ACC) is a well known and typical malignant SGT ACC was previously known as cylindroma in view of its marked histological resemblance to dermal cylindroma. In this study, the expressions of CYLD and NF-kappaB mRNA in HSG, a human SGT cell line, were found to be increased by TNF-alpha stimulation. Immunohistochemistry clearly demonstrated the expression of CYLD and NF-kappaB-related factors in ACC tissue.
authors.affiliation: Second Division of Oral and Maxillofacial Surgery, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama, Japan. fukudam@dent.meikai.ac.jp
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Fukuda
authors.forename: Fumie
authors.initials: F
authors.lastname: Fukuda
authors.forename: Yuko
authors.initials: Y
authors.lastname: Horiuchi
authors.forename: Yuka
authors.initials: Y
authors.lastname: Oku
authors.forename: Seiji
authors.initials: S
authors.lastname: Suzuki
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kusama
authors.forename: Hideaki
authors.initials: H
authors.lastname: Sakashita
mesh_terms: D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D000074749:Deubiquitinating Enzyme CYLD; D016147:Genes, Tumor Suppressor; D006367:HeLa Cells; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D007150:Immunohistochemistry; D008954:Models, Biological; D016328:NF-kappa B / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D025521:Tumor Suppressor Proteins; D025801:Ubiquitin; D051550:I-kappa B Kinase; C409984:CYLD protein, human; D000074749:Deubiquitinating Enzyme CYLD