title: Degradation of extracellular matrix proteins (fibronectin, vitronectin and laminin) by serine-proteinases isolated from Lonomia achelous caterpillar hemolymph.
abstract: Lonomia achelous is a caterpillar distributed in southern Venezuela and in northern Brazil that causes an acute hemorrhagic syndrome in people who have contact with its bristles. The effect of the crude hemolymph and its chromatographic fractions (FDII, Lonomin V and Lonomin V-2) on extracellular matrix proteins was studied. The chromatographic fractions show activities similar to plasmin and urokinase. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis, both lonomins appear as a protein band of 25 kDa under reduced conditions. By exclusion chromatography, the molecular weights of Lonomin V and Lonomin V-2 were 26.5 and 24.5 kDa, respectively. Fibronectin, laminin and vitronectin were degraded by all venom components. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, under reduced conditions, shows that lonomins degrade fibronectin in four main fragments of 116, 60, 50 and 30 kDa. Molecular exclusion chromatography in native conditions shows that the molecular masses of these fragments are > or = 300, 62 and 27 kDa. The proteolytic effect of lonomins was abolished by benzamidine/HCl, iodoacetic acid and aprotinin. The extracellular matrix protein degradation together with the fibrino(geno)lytic activity of hemolymph and its fractions could explain, in part, the hemorrhagic syndrome, and the wound dehiscence in persons who have had contact with the L. achelous caterpillar.
authors.affiliation: Instituto Venezolano de Investigaciones Científicas, Centro de Medicina Experimental, Caracas, Venezuela.
authors.forename: Sara
authors.initials: S
authors.lastname: Lucena
authors.forename: Belsy
authors.initials: B
authors.lastname: Guerrero
authors.forename: Ana M
authors.initials: AM
authors.lastname: Salazar
authors.forename: Amparo
authors.initials: A
authors.lastname: Gil
authors.forename: Carmen L
authors.initials: CL
authors.lastname: Arocha-Piñango
mesh_terms: D000818:Animals; D001180:Arthropod Venoms / Q000302:isolation & purification / Q000494:pharmacology*; D002847:Chromatography, Agarose; D016326:Extracellular Matrix Proteins / Q000187:drug effects*; D005353:Fibronectins / Q000187:drug effects; D006458:Hemolymph / Q000201:enzymology*; D006470:Hemorrhage / Q000139:chemically induced / Q000201:enzymology; D006487:Hemostasis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007797:Laminin / Q000187:drug effects; D009036:Moths / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000302:isolation & purification / Q000494:pharmacology*; D019096:Vitronectin / Q000187:drug effects
chemical_list: D001180:Arthropod Venoms; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D019096:Vitronectin; C108044:lonomin V; D012697:Serine Endopeptidases