Procoagulant activity of surface-immobilized Hageman factor.
Procoagulant activity of surface-immobilized coagulation factor XIIa (activated Hageman factor) is reported. Activity of FXIIa immobilized onto the surfaces of three silanized-glass procoagulants spanning a wide range of wettability was assayed in normal and FXII-deficient plasmas. Previously published mathematical models were used to characterize the procoagulant activity of protein-immobilized materials and soluble enzymes. Results show that FXIIa activity is unrelated to underlying procoagulant surface chemistry and is similar to soluble FXIIa activity. The uninfluential role of the surface on FXIIa suggests that the solid surface activates FXII in biomaterial-induced blood coagulation but is not otherwise involved in FXIIa activity as described by the classical mechanism.
Chatterjee
Kaushik
K
Department of Bioengineering, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
Vogler
Erwin A
EA
Siedlecki
Christopher A
CA
D000327:Adsorption; D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D001792:Blood Platelets / Q000378:metabolism; D002417:Cattle; D003029:Coagulants / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005174:Factor XII / Q000378:metabolism*; D006801:Humans; D008433:Mathematics; D008962:Models, Theoretical; D010949:Plasma / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013499:Surface Properties; D017155:Wettability
D003029:Coagulants; D004800:Enzymes, Immobilized; D012710:Serum Albumin, Bovine; D005174:Factor XII