Functional splice variants of the type II G protein-coupled receptor (VPAC2) for vasoactive intestinal peptide in mouse and human lymphocytes.
A PCR-based search for splice variants of the VPAC2 G protein-coupled receptor for vasoactive intestinal peptide (VIP) revealed: (a) a short-deletion variant in mouse lymphocytes termed VPAC2de367-380, that lacks 14 amino acids in the seventh transmembrane domain, and (b) a long-deletion variant in human lymphocytes termed VPAC2de325-438(i325-334), that lacks 114 amino acids beginning with the carboxyl-terminal end of the third cytoplasmic loop and has 10 new carboxy-terminal amino acids. VPAC2de367-380 binds VIP normally, but shows reduced VIP-evoked signaling and effects on immune functions, whereas VPAC2de325-438(i325-334) shows reduced binding affinity for VIP and a complex pattern of functional differences. These splice variants may modify the immunoregulatory contributions of the VIP-VPAC2 axis.
Miller
Allison L
AL
Department of Medicine, University of California Medical Center, Room UB8B, UC Box 0711, 533 Parnassus at 4th, San Francisco, CA 94143-0711, USA.
Verma
Deepti
D
Grinninger
Carola
C
Huang
Mei-Chuan
MC
Goetzl
Edward J
EJ
D000595:Amino Acid Sequence; D000818:Animals; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D012326:RNA Splicing / Q000235:genetics*; D051239:Receptors, Vasoactive Intestinal Peptide, Type II / Q000737:chemistry / Q000235:genetics*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D014660:Vasoactive Intestinal Peptide