Optimized adaptor polymerase chain reaction method for efficient genomic walking.
Genomic walking is one of the most useful approaches in genome-related research. Three kinds of PCR-based methods are available for this purpose. However, none of them has been generally applied because they are either insensitive or inefficient. Here we present an efficient PCR protocol, an optimized adaptor PCR method for genomic walking. Using a combination of a touchdown PCR program and a special adaptor, the optimized adaptor PCR protocol achieves high sensitivity with low background noise. By applying this protocol, the insertion sites of a gene trap mouse line and two gene promoters from the incompletely sequenced Xenopus laevis genome were successfully identified with high efficiency. The general application of this protocol in genomic walking was promising.
Xu
Peng
P
Laboratory of Molecular and Cell Biology and Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Hu
Rui-Ying
RY
Ding
Xiao-Yan
XY
D000818:Animals; D015820:Cadherins / Q000235:genetics; D003001:Cloning, Molecular; D005822:Genetic Vectors; D023281:Genomics / Q000379:methods*; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic; D000091363:Protocadherins; D029867:Xenopus Proteins / Q000235:genetics; D014982:Xenopus laevis
D015820:Cadherins; C117635:Pcdh8 protein, Xenopus; D000091363:Protocadherins; D029867:Xenopus Proteins