Simplified techniques in rat heterotopic small bowel transplantation.
OBJECTIVE
Establish a simplified heterotopic small bowel transplantation (SBT) in the rat.


METHODS
Ninety pairs of male Wistar rats were used as donors and recipients. The whole small intestine with a vascular pedicle composed of superior mesenteric artery (SMA) and portal vein (PV) was harvested as the graft. Revascularization was accomplished by end-to-side anastomosis between donor SMA and recipient infrarenal aorta and cuffed end-to-end anastomosis between donor PV and left renal vein of recipient. The distal end of graft was exteriorized to form an enterostoma.


RESULTS
Average time of an operation was 130 minutes and the mean warm ischemia time of grafts was 30 minutes. The technical success rate of this model was 100% and 7-day survival was 95.6% (86/90).


CONCLUSIONS
This simplified technique was effective and practical to improve the outcome of rat heterotopic SBT.
Zhang
X-Q
XQ
Department of General Surgery, General Hospital of Jinan Command, Shandong, China.
Sun
J-L
JL
Ma
R
R
Fan
Z-Y
ZY
Dai
Y
Y
Sun
J-Z
JZ
D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D007421:Intestine, Small / Q000637:transplantation; D017538:Mesenteric Artery, Superior / Q000601:surgery; D011169:Portal Vein / Q000601:surgery; D051381:Rats; D016042:Transplantation, Heterotopic; D016896:Treatment Outcome