[Screening differentially expressed genes in human bone marrow stromal cells at defined stage of differentiation.].
To screen differentially expressed genes involved in osteogenic differentiation of human bone marrow stromal cells (BMSCs) at defined stages, subtractive cDNA library was established by means of suppression subtractive hybridization. The BMSCs cultured for 12 and 21 d were used as driver and tester, respectively. A subtract library was successfully constructed and five positive clones were selected from the library. Sequencing analysis and homology comparison showed that the five clones differentially expressed in BMSCs cultured for 21 d were at least 90% homologous with the known genes in human GenBank. It was interestingly found that the osteogenic BMSCs cultured for 21 d differentially expressed decorin and Bax inhibitor 1. RT-PCR was performed to confirm the differentially expressed genes. The results showed that the expression of Bax inhibitor 1 was significantly higher in the cells of 21-day than that of 12-day, while the expression of decorin was only detected in the cells of 21-day.
Liu
Mei-Ling
ML
National Population Research Institute for Family Planning, Beijing, China.
Shi
Xin-Quan
XQ
Zhou
Wan-Hao
WH
Liu
Hong-Wen
HW
Li
Dong
D
Jia
Meng-Chun
MC
D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D058575:Decorin / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*
D051017:Apoptosis Regulatory Proteins; C546878:DCN protein, human; D058575:Decorin; D008565:Membrane Proteins; C484209:TMBIM6 protein, human