title: Augmentation of tendon attachment to porous ceramics by bone marrow stromal cells in a rabbit model.
abstract: Tendon attachment to interconnected porous calcium hydroxyapatite ceramics (IP-CHA) with cultured bone marrow stromal cells (BMSC) was analysed. The purpose of this study was to evaluate whether BMSC in IP-CHA could augment the tendon attachment to IP-CHA histologically and biomechanically. Eighteen Japanese white rabbits were used. Cultured BMSCs were subcultured in IP-CHA. The grafted tendon and IP-CHA with BMSC complex were implanted in a bone defect of the knee [BMSC(+) group]. In the contralateral knee, a tendon and IP-CHA without BMSC complex were implanted [BMSC(-) group]. Histological findings of the interface between the tendon and IP-CHA were similar in the two groups 3 weeks after the operation. However, 6 weeks after the operation, more abundant bone formation around the tendon was observed in the BMSC(+) group. The direct apposition of the tendon to bone in pores and collagen fibre continuity between the tendon and fibrous tissue in pores were observed. In biomechanical evaluation, the maximum pull-out load of the tendon from the IP-CHA in the BMSC(+) group was significantly higher than that in the BMSC(-) group 6 weeks after the operation. BMSCs cultured in IP-CHA could augment tendon attachment to IP-CHA.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Hiroshima 734-8551, Japan. omae@hiroshima-u.ac.jp
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Omae
authors.forename: Yu
authors.initials: Y
authors.lastname: Mochizuki
authors.forename: Shin
authors.initials: S
authors.lastname: Yokoya
authors.forename: Nobuo
authors.initials: N
authors.lastname: Adachi
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D001854:Bone Marrow Cells; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D017886:Durapatite; D020007:Implants, Experimental; D007719:Knee Joint / Q000601:surgery; D059630:Mesenchymal Stem Cells; D016062:Porosity; D011817:Rabbits; D013710:Tendons / Q000473:pathology / Q000637:transplantation*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D017886:Durapatite