title: Cross talk between PML and p53 during poliovirus infection: implications for antiviral defense.
abstract: PML nuclear bodies (NBs) are dynamic intranuclear structures harboring numerous transiently or permanently localized proteins. PML, the NBs' organizer, is directly induced by interferon, and its expression is critical for antiviral host defense. We describe herein the molecular events following poliovirus infection that lead to PML-dependent p53 activation and protection against virus infection. Poliovirus infection induces PML phosphorylation through the extracellular signal-regulated kinase pathway, increases PML SUMOylation, and induces its transfer from the nucleoplasm to the nuclear matrix. These events result in the recruitment of p53 to PML NBs, p53 phosphorylation on Ser15, and activation of p53 target genes leading to the induction of apoptosis. Moreover, the knock-down of p53 by small interfering RNA results in higher poliovirus replication, suggesting that p53 participates in antiviral defense. This effect, which requires the presence of PML, is transient since poliovirus targets p53 by inducing its degradation in a proteasome- and MDM2-dependent manner. Our results provide evidence of how poliovirus counteracts p53 antiviral activity by regulating PML and NBs, thus leading to p53 degradation.
authors.affiliation: CNRS FRE 2944, Institut Lwoff, Villejuif, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Pampin
authors.forename: Yannick
authors.initials: Y
authors.lastname: Simonin
authors.forename: Bruno
authors.initials: B
authors.lastname: Blondel
authors.forename: Yann
authors.initials: Y
authors.lastname: Percherancier
authors.forename: Mounira K
authors.initials: MK
authors.lastname: Chelbi-Alix
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D022003:Cell Nucleus Structures / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism; D010766:Phosphorylation; D017955:Poliovirus / Q000472:pathogenicity*; D000071156:Promyelocytic Leukemia Protein; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D034741:RNA, Small Interfering; D025841:Small Ubiquitin-Related Modifier Proteins; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; C070576:PML protein, human