Fast and precise protein tracking using repeated reversible photoactivation.
Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins' movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here we report 'protein rivers tracking' technique based on repeated identical rounds of photoactivation and subsequent images averaging, which results in dramatic increase of imaging resolution for fast protein movement events.
Chudakov
Dmitriy M
DM
Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, Moscow 117997, Russia Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia. chudakovdm@mail.ru
Chepurnykh
Tatyana V
TV
Belousov
Vsevolod V
VV
Lukyanov
Sergey
S
Lukyanov
Konstantin A
KA
D051023:BH3 Interacting Domain Death Agonist Protein / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D008027:Light; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D051023:BH3 Interacting Domain Death Agonist Protein; C494814:BID protein, human; C485182:Cyan Fluorescent Protein; C430876:FP595 protein, Anemonia sulcata; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins