[A novel binary vector to get marker-free transgenic plant].
A novel practical binary vector to get marker-free transgenic plant was constructed. The estrogen-inducible Cre/loxP DNA recombination system was adopted in this system. All non-target genes located between two identical orientation loxP sites could be excised from the transgenic genome by the Cre expression. In order to analyze this system, the target gene, GUS expression box (CaMV35s: :GUS), was inserted in the MCS outside the region franked by two loxP sites. Then it was introduced into the tobaccos. Results showed that the high-efficiency DNA recombination had take place and the target gene was working order after DNA excitation.
Li
Xia
X
College of Life Sciences of Shanghai University, Shanghai 200436, China.
Weng
Hai-Bo
HB
Han
Shao-Yin
SY
Xi
Yu
Y
Yong
Ke-Lan
KL
D001483:Base Sequence; D005822:Genetic Vectors / Q000235:genetics*; D019426:Integrases / Q000378:metabolism; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics*; D011995:Recombination, Genetic; D014026:Nicotiana / Q000235:genetics
C045073:Cre recombinase; D019426:Integrases