title: Massively parallel signature sequencing.
abstract: Massively parallel signature sequencing is an ultra-high throughput sequencing technology. It can simultaneously sequence millions of sequence tags, and, therefore, is ideal for whole genome analysis. When applied to expression profiling, it reveals almost every transcript in the sample and provides its accurate expression level. This chapter describes the technology and its application in establishing stem cell transcriptome databases.
authors.affiliation: Lynx Therapeutics Inc., Hayward, CA, USA.
authors.forename: Daixing
authors.initials: D
authors.lastname: Zhou
authors.forename: Mahendra S
authors.initials: MS
authors.lastname: Rao
authors.forename: Roger
authors.initials: R
authors.lastname: Walker
authors.forename: Irina
authors.initials: I
authors.lastname: Khrebtukova
authors.forename: Christian D
authors.initials: CD
authors.lastname: Haudenschild
authors.forename: Takumi
authors.initials: T
authors.lastname: Miura
authors.forename: Shannon
authors.initials: S
authors.lastname: Decola
authors.forename: Eric
authors.initials: E
authors.lastname: Vermaas
authors.forename: Keith
authors.initials: K
authors.lastname: Moon
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Vasicek
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D017422:Sequence Analysis, DNA / Q000379:methods; D014158:Transcription, Genetic