title: PACAP and ceramides exert opposite effects on migration, neurite outgrowth, and cytoskeleton remodeling.
abstract: During brain development, cells that fail to reach their final destination or to establish proper connections are eliminated. It has been shown that the proinflammatory cytokine second messenger ceramides and the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) play pivotal roles in the histogenesis of the cerebellum. However, little is known regarding the effects of these two factors on cerebellar granule cell migration. We have found that PACAP prevents the effects of C2-ceramide on granule cell motility and neurite outgrowth. These actions are attributable to opposite effects on actin distribution, tubulin polymerization, and Tau phosphorylation. These data suggest that PACAP and factors inducing ceramide production may control granule cell migration during cerebellar development.
authors.affiliation: INSERM U413, IFRMP 23, University of Rouen, 76821 Mont-Saint-Aignan, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Falluel-Morel
authors.forename: David
authors.initials: D
authors.lastname: Vaudry
authors.forename: Nicolas
authors.initials: N
authors.lastname: Aubert
authors.forename: Ludovic
authors.initials: L
authors.lastname: Galas
authors.forename: Magalie
authors.initials: M
authors.lastname: Benard
authors.forename: Magali
authors.initials: M
authors.lastname: Basille
authors.forename: Marc
authors.initials: M
authors.lastname: Fontaine
authors.forename: Alain
authors.initials: A
authors.lastname: Fournier
authors.forename: Hubert
authors.initials: H
authors.lastname: Vaudry
authors.forename: Bruno J
authors.initials: BJ
authors.lastname: Gonzalez
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D002518:Ceramides / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000494:pharmacology*
chemical_list: D002518:Ceramides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide