title: Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana.
abstract: Thiamin pyrophosphate is an essential coenzyme in all organisms that depend on fermentation, respiration or photosynthesis to produce ATP. It is synthesized through two independent biosynthetic routes: one for the synthesis of 2-methyl-4-amino-5-hydroxymethylpyrimidine pyrophosphate (pyrimidine moiety) and another for the synthesis of 4-methyl-5-(beta-hydroxyethyl) thiazole phosphate (thiazole moiety). Herein, we will describe the three-dimensional structure of THI1 protein from Arabidopsis thaliana determined by single wavelength anomalous diffraction to 1.6A resolution. The protein was produced using heterologous expression in bacteria, unexpectedly bound to 2-carboxylate-4-methyl-5-beta-(ethyl adenosine 5-diphosphate) thiazole, a potential intermediate of the thiazole biosynthesis in Eukaryotes. THI1 has a topology similar to dinucleotide binding domains and although details concerning its function are unknown, this work provides new clues about the thiazole biosynthesis in Eukaryotes.
authors.affiliation: Departamento de Física e Informática, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP, CP 369, 13560-970, Brazil.
authors.forename: Paulo H C
authors.initials: PH
authors.lastname: Godoi
authors.forename: Rodrigo S
authors.initials: RS
authors.lastname: Galhardo
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Luche
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Van Sluys
authors.forename: Carlos F M
authors.initials: CF
authors.lastname: Menck
authors.forename: Glaucius
authors.initials: G
authors.lastname: Oliva
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D004926:Escherichia coli / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013835:Thiamine Pyrophosphate / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
chemical_list: D029681:Arabidopsis Proteins; C437085:THI1 protein, Arabidopsis; D013844:Thiazoles; D000255:Adenosine Triphosphate; D013835:Thiamine Pyrophosphate