[Western-blot with VLSE protein and "in vivo" antigens in Lyme borreliosis diagnosis].
The aim of the study was the evaluation of the efficiency of Western blot (EcoLine) test detecting simoultanous presence of IgM and IgG antibodies against B. burgdorferi in diagnosis of early and late stage of Lyme borreliosis. The comparison of results achieved by performing test Western-blot, ELISA (based on recombinant antigens of three genospecies of Borrelia) and EIA (based on antigens of one B. burgdorferi genospecies). The tests Western blot: EcoLine (Virotech) with antygens "in vivo", ELISA Borrelia IgM, IgG recombinant (Biomedica), EIA: B. b. ss. IgG, EIA B. garinii IgG, EIA B. afzelii IgG (TestLine) were used. Results showed efficacy of detecting IgM, IgG antibodies against VlsE simultanously and IgG antibodies against "in vivo" antigens in diagnosis of early stages of Lyme disease when atypical picture skin lessions arise diagnostic doubts and in discerning early and late stage of disease. The EIA tests based on one B. burgdoreferi genospecies seem less effective in comparison to ELISA tests based on 3 genospecies antigens.
Zajkowska
Joanna
J
Klinika Chorób Zakainych i Neuroinfekcji AM w Białymstoku.
Kondrusik
Maciej
M
Pancewicz
Sławomir
S
Grygorczuk
Sambor
S
Swierzbińska
Renata
R
Hermanowska-Szpakowicz
Teresa
T
Czeczuga
Anna
A
Sienkiewicz
Iwona
I
D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D025065:Borrelia burgdorferi / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D015787:Erythema Chronicum Migrans / Q000175:diagnosis; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008074:Lipoproteins / Q000378:metabolism*; D008193:Lyme Disease / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D020852:Lyme Neuroborreliosis / Q000175:diagnosis; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008074:Lipoproteins; C105546:VlsE protein, Borrelia burgdorferi