Activation of bone marrow cells treated with Canova in vitro.
Canova is a Brazilian complex homeopathic medication produced from Aconitum, Thuya, Bryonia, Lachesis and Arsenicum. Previous studies demonstrated that Canova induces up-regulation in numbers of leukocytes. The bone marrow microenvironment is composed of growth factors, stromal cells, extracellular matrix, and progenitor cells that differentiate into mature blood cells. As it is the major site of blood cell formation, we studied in vitro Canova effects on bone marrow cells of mice. Swiss mouse femurs were dissected, cleaned, and the marrow was flushed. The cells were plated, treated or not, incubated for different times and processed for light, scanning electron, and confocal microscopy, and also flow cytometry. The treatment did not modify the expression of the analyzed surface markers or cytokine production. All microscopy techniques showed that a monocytic lineage (CD11b(+)) and stromal cells (adherent cells) were activated by treatment. Canova also increased cell clusters over adherent cells, suggesting proliferation areas.
Abud
Ana Paula Ressetti
AP
Setor de Ciências Biológicas, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brazil.
Cesar
Beatriz
B
Cavazzani
Luiz Felipe Moscaleski
LF
de Oliveira
Carolina Camargo
CC
Gabardo
Juarez
J
Buchi
Dorly de Freitas
Dde F
D000818:Animals; D001788:Blood Grouping and Crossmatching; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003435:Crotalid Venoms / Q000494:pharmacology*; D016207:Cytokines / Q000032:analysis; D005269:Femur / Q000166:cytology; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D010936:Plant Extracts / Q000494:pharmacology*
C513575:Canova medication; D003435:Crotalid Venoms; D016207:Cytokines; D010936:Plant Extracts