title: Routine culture and differentiation of human embryonic stem cells.
abstract: Human embryonic stem cells provide both an in vitro model of human development and a potential source of cells for treatment of degenerative, metabolic, or traumatic disorders. This chapter describes techniques for routine maintenance and differentiation of human embryonic stem cells in culture.
authors.affiliation: The Roslin Institute Department of Gene Function and Development, Scotland, UK.
authors.forename: Jim
authors.initials: J
authors.lastname: McWhir
authors.forename: Davina
authors.initials: D
authors.lastname: Wojtacha
authors.forename: Alison
authors.initials: A
authors.lastname: Thomson
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002455:Cell Division; D018920:Coculture Techniques / Q000379:methods; D003094:Collagen; D015925:Cryopreservation; D017077:Culture Media, Conditioned; D004338:Drug Combinations; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007621:Karyotyping; D007797:Laminin; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology*; D011509:Proteoglycans; D014357:Trypsin
chemical_list: D001672:Biocompatible Materials; D017077:Culture Media, Conditioned; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D014357:Trypsin