title: The association of newly identified respiratory viruses with lower respiratory tract infections in Korean children, 2000-2005.
abstract: BACKGROUND
  This study was performed to evaluate the associations of newly recognized viruses, namely, human metapneumovirus (hMPV), human coronavirus (HCoV)-NL63, and human bocavirus (HBoV) with lower respiratory tract infections (LRTIs) in previously healthy children.
  
  
  METHODS
  To determine the prevalences of 11 viruses--respiratory syncytial virus (RSV), adenovirus, rhinovirus, parainfluenza viruses (PIVs) 1 and 3, influenza viruses A and B, hMPV, HCoV, HCoV-NL63, and HBoV--among infants or children with LRTIs, in association with their epidemiologic characteristics, we performed multiplex reverse-transcriptase polymerase chain reaction on nasopharyngeal aspirates obtained from 515 children < or =5 years old with LRTIs during the period 2000-2005.
  
  
  RESULTS
  Viruses were identified in 312 (60.6%) of the 515 patients. RSV was detected in 122 (23.7%), HBoV in 58 (11.3%), adenovirus in 35 (6.8%), PIV-3 in 32 (6.2%), rhinovirus in 30 (5.8%), hMPV in 24 (4.7%), influenza A in 24 (4.7%), PIV-1 in 9 (1.7%), influenza B in 9 (1.7%), and HCoV-NL63 in 8 (1.6%). Coinfections with > or =2 viruses were observed in 36 patients (11.5%). Twenty-two patients (37.9%) infected with HBoV had a coinfection. Bronchiolitis was frequently diagnosed in patients who tested positive for RSV, PIV-3, or rhinovirus, whereas influenza A, PIV-1, and HCoV-NL63 were commonly found in patients with croup. The age distributions of patients with viral infections differed; notably, RSV was responsible for 77% of LRTIs that occurred in infants < or =3 months old. The number of hMPV infections peaked between February and April, whereas the number of HCoV-NL63 infections peaked between April and May.
  
  
  CONCLUSIONS
  This study describes the features of LRTIs associated with newly identified viruses in children, compared with those associated with known viruses. Additional investigations are required to define the role of HBoV in LRTI.
authors.affiliation: Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Eun Hwa
authors.initials: EH
authors.lastname: Choi
authors.forename: Hoan Jong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sun Jung
authors.initials: SJ
authors.lastname: Kim
authors.forename: Byung Wook
authors.initials: BW
authors.lastname: Eun
authors.forename: Nam Hee
authors.initials: NH
authors.lastname: Kim
authors.forename: Jin A
authors.initials: JA
authors.lastname: Lee
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Song
authors.forename: So Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Ji Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Sung
mesh_terms: D002675:Child, Preschool; D006801:Humans; D007223:Infant; D007723:Korea / Q000453:epidemiology; D014777:Virus Diseases / Q000453:epidemiology* / Q000821:virology*; D014780:Viruses / Q000145:classification* / Q000302:isolation & purification*