VPAC2 receptor activation mediates VIP enhancement of population spikes in the CA1 area of the hippocampus.
The receptors mediating vasoactive intestinal polypeptide (VIP) enhancement of synaptic transmission to pyramidal cell bodies were investigated. RO 25-1553 (VPAC2agonist) mimicked the excitatory effect of VIP on population spike (PS) amplitude. [K15, R16, L27] VIP (1-7)/GRF (8-27) (VPAC1 agonist) caused only a small increase in PS amplitude. The effect of VPAC2 agonist (but not of the VPAC1 agonist) persisted upon blockade of GABAergic transmission and was strongly attenuated upon inhibition of PKA. In conclusion, VPAC2 receptor activation mediates VIP enhancement of PS amplitude in the hippocampus essentially through a PKA-dependent mechanism.
Cunha-Reis
Diana
D
Institute of Pharmacology and Neurosciences, Faculty of Medicine and Institute of Molecular Medicine, University of Lisbon, Av. Prof. Egas Moniz, 1649-028 Lisbon, Portugal. diana-reis@fm.ul.pt
Ribeiro
Joaquim Alexandre
JA
Sebastião
Ana M
AM
D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004594:Electrophysiology; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D051239:Receptors, Vasoactive Intestinal Peptide, Type II / Q000378:metabolism*; D015398:Signal Transduction; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D014660:Vasoactive Intestinal Peptide; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases