Comparison of real-time PCR assays for monitoring serum hepatitis B virus DNA levels during antiviral therapy.
The performance characteristics of the RealART and Molecular Beacons assays were compared with those of the Digene Hybrid Capture II assay (ultrasensitive). The results of the RealART and Digene Hybrid assays were related (r = 0.94; P < 0.001) and diverged by 2 orders of magnitude. The RealART assay can be used to effectively monitor serum hepatitis B virus DNA levels.
Hui
Chee-Kin
CK
Department of Medicine, University of Hong Kong, Hong Kong, SAR, China.
Bowden
Scott
S
Zhang
Hai-Ying
HY
Wong
Anita
A
Lewin
Sharon
S
Rousseau
Frank
F
Mommeja-Marin
Herve
H
Lee
Nikki P
NP
Luk
John M
JM
Locarnini
Stephen
S
Leung
Nancy
N
Naoumov
Nikolai V
NV
Lau
George K K
GK
D004279:DNA, Viral / Q000032:analysis*; D016903:Drug Monitoring / Q000379:methods*; D006509:Hepatitis B / Q000188:drug therapy* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019562:Viral Load
D004279:DNA, Viral