The effect of ultrasound stimulation versus bioreactors on neocartilage formation in tissue engineering scaffolds seeded with human chondrocytes in vitro.
Pulsed ultrasound (1 MHz, 67 mW/cm(2) Ispta, and 10 min/day) promoted cell proliferation and matrix deposition in low-density 2D ( approximately 6 x 10(3)cells/cm(2)) as well as 3D ( approximately 4 x 10(6)cells/cm(3)) chondrocyte cultures. The beneficial effect of ultrasound on neocartilage formation only last 28 days, shorter than that of bioreactors.
Hsu
Shan-hui
SH
Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan, ROC. shhsu@nchu.edu.tw <shhsu@nchu.edu.tw>
Kuo
Chi-Ching
CC
Whu
Shu Wen
SW
Lin
Chen-Huan
CH
Tsai
Ching-Lin
CL
D019149:Bioreactors; D002358:Cartilage, Articular / Q000166:cytology / Q000254:growth & development* / Q000528:radiation effects; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology* / Q000528:radiation effects; D020219:Chondrogenesis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology* / Q000528:radiation effects; D010812:Physical Stimulation / Q000379:methods; D013010:Sonication; D023822:Tissue Engineering / Q000379:methods*