title: Dodeca-CLE peptides as suppressors of plant stem cell differentiation.
abstract: In plants and animals, small peptide ligands that signal in cell-cell communication have been suggested to be a crucial component of development. A bioassay of single-cell transdifferentation demonstrates that a dodecapeptide with two hydroxyproline residues is the functional product of genes from the CLE family, which includes CLAVATA3 in Arabidopsis. The dodecapeptide suppresses xylem cell development at a concentration of 10(-11) M and promotes cell division. An application, corresponding to all 26 Arabidopsis CLE protein family members, of synthetic dodecapeptides reveals two counteracting signaling pathways involved in stem cell fate.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Ito
authors.forename: Ikuko
authors.initials: I
authors.lastname: Nakanomyo
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Motose
authors.forename: Kuninori
authors.initials: K
authors.lastname: Iwamoto
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Sawa
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism; D019659:Asteraceae / Q000166:cytology*; D001483:Base Sequence; D001681:Biological Assay; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D008024:Ligands; D018519:Meristem / Q000166:cytology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development; D018514:Plant Structures / Q000166:cytology*; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology*
chemical_list: C118167:AT2G27250 protein, Arabidopsis; D029681:Arabidopsis Proteins; D008024:Ligands; D009842:Oligopeptides; D010940:Plant Proteins