Enhanced sensitivity and stability in two-color in situ hybridization by means of a novel chromagenic substrate combination.
Double in situ hybridization analysis is a fundamental technique for studying the expression of two genes with high temporal and spatial resolution. However, due to the lack of sensitivity in current detection methods, this approach is powerful only when at least one transcript is abundantly expressed. Here, we report a new enzyme/chromagenic substrate combination that provides sufficient sensitivity for detecting two less abundant transcripts and stability for subsequent paraffin sectioning.
Hurtado
Romulo
R
University of California San Francisco, Cardiovascular Research Institute and Department of Anatomy, San Francisco, California, USA.
Mikawa
Takashi
T
D000818:Animals; D002642:Chick Embryo; D002863:Chromogenic Compounds / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D017403:In Situ Hybridization / Q000379:methods*; D016612:Paraffin Embedding / Q000379:methods; D015347:RNA Probes / Q000235:genetics; D015203:Reproducibility of Results
D002863:Chromogenic Compounds; D015347:RNA Probes