[Enhancement of laboratory diagnosis specificity in human toxocariasis].
The aim of the study was to enhance specificity of immunological diagnosis in toxocarosis. The investigations concern: detection of circulating parasite antigens using dot-ELISA technique and detection of specific IgG antigodies using ELISA test with low molecular weight (< or = 30 kD) excretory-secretory (ES) larval T. canis antigens. The circulating antigens were investigated in 60 sera and 20 anterior chamber fluids from children with ocular form of toxocarosis. These antigens were present only in 3 sera and 2 anterior chamber fluids in children with active intraocular inflamation and fluid antibodies titers 1:5120 and 1:20 000. Comparative study with different ELISA tests indicated that use of low molecular weight antigens increase specificity of reaction.
Zarnowska-Prymek
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Klinika Chorób Odzwierzecych i Tropikaknych AM, 01-201 Warszawa, ul. Wolska 37.
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007815:Larva Migrans / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D017166:Toxocara canis / Q000502:physiology*; D014120:Toxocariasis / Q000175:diagnosis* / Q000469:parasitology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G