Expression of DNMT-1 in patients with atopic dermatitis.
DNA methylation is known to play an important role in gene transcription and alterations of methylation that contribute to the development of certain disorders such as cancer, immunodeficiency, and autoimmune diseases. We investigated the DNA methylation profiles in patients with atopic dermatitis (AD). Messenger RNA (mRNA) levels for DNA methyltransferase-1 (DNMT-1) in peripheral blood mononuclear cells (PBMC) were examined using a real-time quantitative polymerase chain reaction method. The levels of DNMT-1 mRNA were significantly lower in PBMC from the AD patients who had higher serum IgE levels compared with normal controls. Our observations suggest that suppression of DNMT-1 might be related to the pathogenesis of AD, especially in whom serum IgE level is high. This is the first report of DNMT-1 expression in AD patients.
Nakamura
Toshinobu
T
Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan. azki@med.juntendo.ac.jp
Sekigawa
Iwao
I
Ogasawara
Hitoshi
H
Mitsuishi
Kouichi
K
Hira
Kayako
K
Ikeda
Shigaku
S
Ogawa
Hideoki
H
D000293:Adolescent; D000328:Adult; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D007073:Immunoglobulin E; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases