title: [Hemangiopoiet in modulates adhesive properties of endothelial cells].
abstract: OBJECTIVE
  To explore the effect of hemangiopoietin (HAPO) on the adhesive properties of human umbilical vein endothelial cells (HUVEC).
  
  
  METHODS
  The adhesion of HUVEC and the expressions of CD54, CD102, CD106, CD31, CD62E, and CD62P were measured by adhesion assay, flow cytometry, and semi-quantitative RT-PCR.
  
  
  RESULTS
  HAPO enhanced the total adherence of HUVEC in a concentration-dependent manner. Flow cytometry analysis revealed that the treatment of HAPO resulted in a significantly increased expression of CD106 and CD62E on HUVEC in a time-dependent manner. When HUVEC were incubated with HAPO for 6 h, the percentage of CD106 + HUVEC and CD62E HUVEC increased about 2.10 folds and 5.84 folds, respectively, compared with control. The time-course of adhesive molecules mRNA expression indicated that the expression of CD106 and CD62E reached at the maximum 1.86 folds and 6.16 folds, respectively, compared with control.
  
  
  CONCLUSIONS
  HAPO may facilitate the homing of hematopoietic stem/progenitor cells.
authors.affiliation: State Key Laboratory of Experimental Hematology, Tianjin Economic-Technological Development Area Life and Technology Research Center, Institute of Hematology, CAMS and PUMC, Tianjin 300020, China.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Liu
authors.forename: Shi-Hong
authors.initials: SH
authors.lastname: Lu
authors.forename: Lin-Hui
authors.initials: LH
authors.lastname: Liang
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.forename: Zhong-Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D019040:E-Selectin / Q000096:biosynthesis / Q000235:genetics; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D011509:Proteoglycans / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D000099158:Proteoglycan 4
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1; D011509:Proteoglycans; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; C419197:PRG4 protein, human; D000099158:Proteoglycan 4