title: Characterization of emulsified chitosan-PLGA matrices formed using controlled-rate freezing and lyophilization technique.
abstract: This study evaluated the formation of chitosan-50:50 poly-lactic-co-glycolic acid (PLGA) blend matrices using controlled-rate freezing and lyophilization technique (CRFLT). An emulsion system was used in the presence of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), a cellular component, as a stabilizer. Blended scaffolds showed an open pore morphology and homogenous interdispersion of PLGA and chitosan. Forming emulsions after dissolving PLGA in chloroform, benzene, or methylene chloride indicated better emulsion stability with benzene and chloroform. Scaffolds formed by freezing at -20, -78, and -196 degrees C from these emulsions showed significant influence of the solvent and freezing temperature on the microarchitecture of the scaffold. By controlling the concentration of chitosan, scaffolds with greater than 90% porosity were attained. Since the two polymers degrade by different mechanisms, formed scaffolds were analyzed for degradation characteristics for 4 weeks in presence of 10 mg/L lysozyme. These results showed no significant difference in the weight loss and dimension changes, as all scaffolds showed significant (a) weight loss and (b) nearly 60% reduction in volume. Further, pH of the incubation media decreased in all the samples. When cellular activity of green fluorescence protein-transfected smooth muscle cells was analyzed, no apparent cytotoxicity was observed. However, the cell spreading area decreased. In summary, these results show promising potential in tissue engineering and drug delivery applications.
authors.affiliation: School of Chemical Engineering, Oklahoma State University, Stillwater, 74078, USA.
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Moshfeghian
authors.forename: Jeremy
authors.initials: J
authors.lastname: Tillman
authors.forename: Sundararajan V
authors.initials: SV
authors.lastname: Madihally
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D016503:Drug Delivery Systems; D005612:Freeze Drying / Q000379:methods*; D005615:Freezing; D049452:Green Fluorescent Proteins / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D015233:Models, Statistical; D009113:Muramidase / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D012997:Solvents; D049452:Green Fluorescent Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan; C421864:hen egg lysozyme; D009113:Muramidase; D004134:Dimyristoylphosphatidylcholine