title: High-throughput and high-resolution flow cytometry in molded microfluidic devices.
abstract: We describe the design, fabrication, and operation of two types of flow cytometers based on microfluidic devices made of a single cast of poly(dimethylsiloxane). The stream of particles or cells injected into the devices is hydrodynamically focused in both transverse and lateral directions, has a uniform velocity, and has adjustable diameter and shape. The cytometry system built around the first microfluidic device has fluorescence detection accuracy comparable with that of a commercial flow cytometer and can analyze as many as 17 000 particles/s. This high-throughput microfluidic device could be used in inexpensive stand-alone cytometers or as a part of integrated microanalysis systems. In the second device, a stream of particles is focused to a flow layer of a submicrometer thickness that allows imaging the particles with a high numerical aperture microscope objective. To take long-exposure, low-light fluorescence images of live cells, the device is placed on a moving stage, which accurately balances the translational motion of particles in the flow. The achieved resolution is comparable to that of still micrographs. This high-resolution device could be used for analysis of morphology and fluorescence distribution in cells in continuous flow.
authors.affiliation: Department of Physics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA.
authors.forename: Claire
authors.initials: C
authors.lastname: Simonnet
authors.forename: Alex
authors.initials: A
authors.lastname: Groisman
mesh_terms: D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D005453:Fluorescence; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods