title: Peritoneal perfusion with oxygenated perfluorocarbon augments systemic oxygenation.
abstract: BACKGROUND
  Despite maximal ventilatory support, many patients die from hypoxia in the setting of potentially reversible pulmonary failure. There remains a pressing need for additional pulmonary supportive care measures, especially techniques that do not require systemic anticoagulation. The objective of our experiments was to determine whether systemic oxygenation could be increased in a large animal, with induced hypoxia, by perfusing the abdominal cavity with oxygenated perfluorocarbons.
  
  
  METHODS
  Fifteen pigs with a mean (+/- SD) weight of 45 +/- 5 kg were intubated and rendered hypoxic by ventilating them with a blend of nitrogen and oxygen to achieve subatmospheric concentrations of inspired oxygen ranging from 18 to 10%, resulting in baseline mean Pao(2) range of 65.9 +/- 9.7 to 26.6 +/- 2.8 mm Hg, respectively. Peritoneal perfusion was performed in eight animals with oxygenated perfluorocarbon and in seven control animals with oxygenated saline solution.
  
  
  RESULTS
  The average increase in Pao(2) with oxygenated perfluorocarbon perfusion, compared to oxygenated saline solution perfusion, ranged from 8.1 to 18.2 mm Hg. A common treatment effect was estimated across all fraction of inspired oxygen (Fio(2)) values, representing the average mean difference in oxygen uptake between oxygenated perfluorocarbon and saline solution, irrespective of the level of Fio(2). This average was 12.8 mm Hg (95% confidence interval, 7.4 to 18.2; p < 0.001). The most clinically relevant results occurred at an Fio(2) of 14%, resulting in a baseline mean Pao(2) of 39.4 +/- 5.0 mm Hg with oxygenated saline solution perfusion, and a mean Pao(2) of 55.3 +/- 7.6 mm Hg with oxygenated perfluorocarbon perfusion. This corresponded to an increase in arterial oxygen saturation from 73 to 89%.
  
  
  CONCLUSIONS
  These results of our principle experiments demonstrate that the peritoneal cavity can be used for gas exchange and, in our model, yielded clinically relevant increases in systemic arterial oxygen levels. This technique may have the potential for the supportive care of patients dying from hypoxia in the setting of reversible lung injury.
authors.affiliation: Department of Surgery, University of Pennsylvania, Philadelphia, 19104, USA.
authors.forename: Shamus R
authors.initials: SR
authors.lastname: Carr
authors.forename: Joshua P
authors.initials: JP
authors.lastname: Cantor
authors.forename: Atul S
authors.initials: AS
authors.lastname: Rao
authors.forename: Thiru V
authors.initials: TV
authors.lastname: Lakshman
authors.forename: Joshua E
authors.initials: JE
authors.lastname: Collins
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Friedberg
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015199:Extracorporeal Membrane Oxygenation; D005466:Fluorocarbons / Q000008:administration & dosage*; D000860:Hypoxia / Q000628:therapy*; D010100:Oxygen / Q000008:administration & dosage*; D010477:Perfusion / Q000379:methods*; D010537:Peritoneum; D013552:Swine; D016896:Treatment Outcome
chemical_list: D005466:Fluorocarbons; D010100:Oxygen