Phylogenetic analysis of enteric species of the family Enterobacteriaceae using the oriC-locus.
Phylogenetic analysis of 38 enteric species belonging to the Enterobacteraceae family was carried out using the non-coding locus oriC, the chromosomal replication origin. The oriC loci were amplified with conserved oligonucleotides and the PCR fragments were sequenced directly. The results establish a phylogenetic tree for the classification of different species of the genera Escherichia, Shigella, Salmonella, Enterobacter, Citrobacter, Klebsiella, Raoultella, Kluyvera, Cedecea and Buttiauxella. Functional important protein-binding sites located in oriC are well conserved throughout the enteric group. More over, due to a high overall divergence value phylogenetic trees were robust and well supported by bootstrap analysis. In comparison with 16S rDNA analysis, the oriC sequences indicated a greater evolutionary divergence for bacteria. We propose that the oriC locus might be a suitable phylogenetic marker for the identification and classification of bacteria, in particular for closely related species.
Roggenkamp
Andreas
A
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, 81377 Munich, Germany. Andreas.Roggenkamp@labor-schubach.de
D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D051738:Origin Recognition Complex / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C114650:OriC chromosomal replication origin; D051738:Origin Recognition Complex; D012336:RNA, Ribosomal, 16S