[Effect of interferon-gamma on bleomycin induced pulmonary fibrosis in rats].
OBJECTIVE
To observe the effects of interferon-gamma (IFN-gamma) on lung injury induced by bleomycin and its mechanism.


METHODS
Sixty-six SD rats were randomly divided into three group: the normal control group (group N, n=24), the bleomycin group (group B, n=24) and IFN-gamma treatment group (group I, n=18). The degree of lung injury and the concentrations of tumor necrosis factor-alpha (TNF-alpha) and IFN-gamma in bronchoalveolar lavage fluid (BALF) and serum were measured.


RESULTS
The degree of lung injury in group I was severer than group B, but with no significant difference. The concentration of TNF-alpha in BALF on 17, 21, 28 days and in serum on 21 and 28 days were significantly increased after IFN-gamma administration compared with group N and group B (all P<0.05). The concentration of IFN-gamma in BALF and serum increased on 21 and 28 days in group I (all P<0.05).


CONCLUSIONS
IFN-gamma might aggravate lung injury induced by bleomycin, and it might attribute to an increase in TNF-alpha.
Zhang
De-ping
DP
Department of Respiratory Medicine, Drum Tower Hospital, Nanjing University Medical College, Nanjing 210008, Jiangsu, China.
Qiu
Hui
H
Zhuang
Yi
Y
Meng
Fan-qing
FQ
D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004195:Disease Models, Animal; D007371:Interferon-gamma / Q000378:metabolism / Q000633:toxicity*; D008168:Lung / Q000187:drug effects / Q000473:pathology; D055370:Lung Injury / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D014409:Tumor Necrosis Factor-alpha; D001761:Bleomycin; D007371:Interferon-gamma