title: Evidence of oxidative and nitrosative stress in patients with cervical squamous cell carcinoma.
abstract: BACKGROUND
  We conducted a study to evaluate reactive oxygen species (ROS) and reactive nitrogen species (RNS) simultaneously together with the antioxidant status in patients with cervical carcinoma.
  
  
  METHODS
  We measured lipid peroxidation product, including malondialdehye (MDA), nitric oxide (NO) products, including nitrite (NO(2)(-)), nitrate (NO(3)(-)) and total nitrite (TNO(2)(-)) and antioxidant enzymes in 45 patients with cervical cancer and compared them against 30 healthy controls.
  
  
  RESULTS
  Plasma as well as erythrocyte MDA and plasma NO levels was higher (p<0.001) in cervical cancer as compared to healthy controls. Antioxidant enzymes, Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, were decreased (p<0.001) whereas glutathione S-transferase (GST) activity was increased (p<0.001) in cervical cancer patients. Lipid peroxidation and NO products accumulation correlated significantly with a deranged antioxidant system.
  
  
  CONCLUSIONS
  We demonstrated a possible involvement of both oxidative and nitrosative stress, as evidenced by increased lipid peroxidation and NO levels with altered antioxidant defense system in the pathogenesis of cervical cancer.
authors.affiliation: Department of Physiology and Biochemistry, Ragas Dental College and Hospital, Chennai-600 119, Tamil Nadu, India. Sayeedamaricar@yahoo.co.in
authors.forename: Syed Sultan
authors.initials: SS
authors.lastname: Beevi
authors.forename: Muzib Hassanal
authors.initials: MH
authors.lastname: Rasheed
authors.forename: Arumugam
authors.initials: A
authors.lastname: Geetha
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000453:epidemiology; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D006801:Humans; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000453:epidemiology
chemical_list: D009569:Nitric Oxide; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase