Bioenergy beads: a tool for regeneration of ATP/NTP in biocatalytic synthesis.
Active inclusion bodies of recombinant polyphosphate kinase were obtained by simple washing of Escherichia coli cells with nonionic detergent and then they were immobilized in agar/TiO2 beads. Bioenergy beads obtained are charged by polyphosphate to act as rechargeable supply of adenosine/nucleoside triphosphates (ATP/NTP), a practical tool for synthesis of artificial receptors.
Nahálka
Jozef
J
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Gemeiner
Peter
P
Bucko
Marek
M
Wang
Peng George
PG
D000255:Adenosine Triphosphate / Q000378:metabolism*; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000096:biosynthesis / Q000302:isolation & purification*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D002479:Inclusion Bodies / Q000378:metabolism*; D008863:Microspheres; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis / Q000302:isolation & purification*
D004800:Enzymes, Immobilized; D029968:Escherichia coli Proteins; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C475691:polyphosphate kinase, E coli