Bacillus seohaeanensis sp. nov., a halotolerant bacterium that contains L-lysine in its cell wall.
A halotolerant, round-endospore-forming, aerobic, Gram-positive bacterium, designated BH724(T), was isolated from a solar saltern at Taean in Korea. Cells of this strain were rod-shaped and found to be non-motile. Strain BH724(T) grew at salinities of 0-10 % (w/v) NaCl with an optimum of 3 % (w/v) NaCl and at temperatures of 15-50 degrees C with an optimum of 40 degrees C. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BH724(T) belonged to the genus Bacillus and that Bacillus aquimaris TF-12(T), Bacillus marisflavi TF-11(T) and Bacillus vietnamensis JCM 11124(T) were its closest neighbours, sharing 97.3, 97.2 and 97.0 % 16S rRNA gene sequence similarity, respectively. The genomic DNA G+C content was 39 mol% and the predominant menaquinone was MK-7. Its major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0), iso-C(16 : 0) and iso-C(14 : 0). The peptidoglycan type was A1alpha, linked directly through l-lysine. On the basis of morphological, chemotaxonomic, physiological and phylogenetic properties, strain BH724(T) represents a novel species of the genus Bacillus, for which the name Bacillus seohaeanensis sp. nov. is proposed. The type strain is BH724(T) (=KCTC 3913(T)=DSM 16464(T)).
Lee
Jae-Chan
JC
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Lim
Jee-Min
JM
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Park
Dong-Jin
DJ
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Jeon
Che Ok
CO
Division of Environmental Biotechnology, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Kim
Chang-Jin
CJ
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D003470:Culture Media; D003487:Cyanoacrylates / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007218:Industrial Microbiology; D008239:Lysine / Q000032:analysis / Q000378:metabolism; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis / Q000378:metabolism; D010641:Phenotype; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride; D013045:Species Specificity; D013696:Temperature; D024482:Vitamin K 2 / Q000031:analogs & derivatives
D003470:Culture Media; D003487:Cyanoacrylates; D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C007166:cyacrin; D012965:Sodium Chloride; C062629:menaquinone 7; D008239:Lysine