title: A validated method for halquinol extraction from chicken liver matrix for residual HPLC studies.
abstract: Extraction is the key step in the analysis of food samples, from either plant or animal sources for residues of veterinary drugs and pesticides. Such residues pose an unacceptable risk to the consumer and information is insufficient to allow a full assessment. This paper presents a method for extracting and determining Halquinol, a veterinary drug in liver of chicken. The first step of the study was to establish and validate a method for determination of Halquinol, which was achieved using HPLC equipped with a C18 reversed-phase, monomeric column and a MWD-UV detector. In the second step, we extracted and purified the liver samples. Liver samples were homogenized in buffer, extracted with ethyl acetate, and cleaned up using a solid-phase extraction column. The limit of quantification of the method was 0.1 microg/l mL, and detection limit was 0.01 microg/l mL. Mean recoveries in chicken liver ranged from 57% to 91%.
authors.affiliation: Vetcare Research and Development Center, Division of Tetragon Chemie Private Limited, Bangalore -560064, Karnataka, India. mushigerisadanand@rediffmail.com
authors.forename: Sadanand
authors.initials: S
authors.lastname: Mushigeri
authors.forename: M
authors.initials: M
authors.lastname: Ganesh
authors.forename: P M
authors.initials: PM
authors.lastname: Radhakrishna
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D002739:Chloroquinolinols / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000890:Anti-Infective Agents; D002739:Chloroquinolinols; C011838:halquinol