title: [Screen of media for insect cells (HzAm1) growth and its adaptation to low serum culture].
abstract: Insect cell-baculovirus system is a useful tool for both insecticidal virus production and the expression of medically useful foreign genes. Serum-free culture or low serum culture for insect cells is essential and significant. Media for the growth of insect cells HzAm1 from three kinds of commercial media TC-100,GRACE and IPL-41 with 10% serum were investigated and screened. The result shows that medium TC-100 is the most suitable medium for the growth of cells HzAm1. Adaptation of insect cells HzAm1 to cultures in medium TC-100 with serum reduction from 10% to 1% was carried out as cultures were supplemented with lactalbumin hydrolysate and yeastolate etc. Growth of insect cells HzAml in medium TC-100 with serum 1%, lactalbumin hydrolysate and yeastolate was well.
authors.affiliation: Hubei Key Laboratory of Novel Chemical Reactor & Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, China. youhong64@yahoo.com.cn
authors.forename: You-Hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Hua-Lin
authors.initials: HL
authors.lastname: Wang
authors.forename: Xiong-Wei
authors.initials: XW
authors.lastname: Zhu
authors.forename: Qin
authors.initials: Q
authors.lastname: Qin
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhong
authors.initials: Z
authors.lastname: Lü
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D049109:Cell Proliferation; D003470:Culture Media; D016895:Culture Media, Serum-Free; D007915:Lepidoptera / Q000166:cytology*
chemical_list: D003470:Culture Media; D016895:Culture Media, Serum-Free