title: Fully automated analytical method for mycophenolic acid quantification in human plasma using on-line solid phase extraction and high performance liquid chromatography with diode array detection.
abstract: We have developed a new fully automated method for mycophenolic (MPA) acid quantification in plasma to optimize therapeutic drug monitoring of tranplant patients. This method involved solid-phase extraction on disposable extraction cartridges and reversed-phase high-performance liquid chromatography with diode array detection. Solid-phase extraction was performed automatically by an automated sample with extraction catridges system. After washing, MPA was eluted from the cartridge onto a Chromolith RP-18e column. MPA and the internal standard were detected at 306 nm. The retention time of MPA was 6.3 minutes. The developed method was linear from 0.2 to 20 microg/mL. The limit of quantification was 0.2 microg/mL. The method showed a good precision with intraday and interday variation coefficient less than 6%. The intraday accuracy ranged from 97.6% to 100.4% and the interday accuracy varied from 97.1% to 100.8%. The extraction efficiency was greater than 90%. This method is simple and shows a good specificity with respect to commonly co-prescripted drugs.
authors.affiliation: CHU de Bordeaux, Département de Pharmacologie Clinique et de Toxicologie, Bordeaux F33000, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Daurel-Receveur
authors.forename: Karine
authors.initials: K
authors.lastname: Titier
authors.forename: Ste Phane
authors.initials: SP
authors.lastname: Picard
authors.forename: Dominique
authors.initials: D
authors.lastname: Ducint
authors.forename: Nicholas
authors.initials: N
authors.lastname: Moore
authors.forename: Mathieu
authors.initials: M
authors.lastname: Molimard
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000097:blood / Q000302:isolation & purification / Q000627:therapeutic use; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D004355:Drug Stability; D006084:Graft Rejection / Q000517:prevention & control; D006801:Humans; D009173:Mycophenolic Acid / Q000097:blood* / Q000302:isolation & purification / Q000627:therapeutic use; D015203:Reproducibility of Results
chemical_list: D000903:Antibiotics, Antineoplastic; D009173:Mycophenolic Acid