Genomic profiling of cortical neurons following exposure to beta-amyloid.
In vitro and in vivo studies have shown that beta-amyloid peptide induces neuronal cell death. To explore the molecular basis underlying beta-amyloid-induced toxicity, we analyzed gene expression profiles of cultured rat cortical neurons treated for 24 and 48 h with synthetic beta-amyloid peptide. From the 8740 genes interrogated by oligonucleotide microarray analysis, 241 genes were found to be differentially expressed and segregated into distinct clusters. Functional clustering based on gene ontologies showed coordinated expression of genes with common biological functions and metabolic pathways. The comparison with genes differentially expressed in cerebellar granule neurons following serum and potassium deprivation indicates the existence of common regulatory mechanisms underlying neuronal cell death. Our results offer a genomic view of the changes that accompany beta-amyloid-induced neurodegeneration.
Paratore
Sabrina
S
Functional Genomics Center, Institute of Neurological Sciences, Italian National Research Council, Italy.
Parenti
Rosalba
R
Torrisi
Antonietta
A
Copani
Agata
A
Cicirata
Federico
F
Cavallaro
Sebastiano
S
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects; D005260:Female; D020869:Gene Expression Profiling; D009474:Neurons / Q000187:drug effects* / Q000473:pathology / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar
D016229:Amyloid beta-Peptides