Assessment of adrenal reserve in pregnancy: defining the normal response to the adrenocorticotropin stimulation test.
BACKGROUND
Normal pregnancy is a state of hypercortisolism, making adrenal insufficiency difficult to diagnose.


OBJECTIVE
We sought to identify a normative, minimum-response threshold for the ACTH stimulation test in pregnancy. We hypothesized that salivary free cortisol (SaFC) would prove a more physiological and less variable measure of adrenal reserve in pregnancy than serum cortisol (SC).


METHODS
This is a prospective study of normal controls.


METHODS
The study was conducted in an obstetrical clinic in a tertiary care hospital.


METHODS
Patients included 36 healthy ambulatory pregnant women (aged 18-37 yr) with singleton pregnancies.


METHODS
The 250-microg ACTH stimulation test was performed in the healthy pregnant volunteers. Based on their gestational age at the time of recruitment, women were studied in one of the trimesters and were restudied at 11-14 wk postpartum.


METHODS
Total SC, aldosterone, and SaFC concentrations were measured before and after ACTH. The response in pregnancy was compared with postpartum values.


RESULTS
Basal SC (P = 0.01), aldosterone (P = 0.001), and SaFC (P = 0.01) values progressively increased during the trimesters of pregnancy and decreased postpartum, confirming that pregnant women have increased basal glucocorticoid and mineralocorticoid production. There was enhanced responsiveness of the maternal adrenal glands to ACTH stimulation as pregnancy progressed, as measured by peak stimulated SaFC (P = 0.009) and aldosterone (P = 0.01). In the milieu of altered binding globulins, SaFC is a more consistent, binding-globulin-independent measure of stimulated adrenal function than total SC. Minimum criteria for the normal SaFC response to ACTH stimulation in the second and third trimesters of pregnancy and postpartum have been generated based on a predominantly African-American group of subjects.


CONCLUSIONS
Reliable data are available for the evaluation of the adrenal axis in pregnancy with a noninvasive, outpatient measure of SaFC. Glucocorticoid therapy in pregnancy should take into account that adrenal reserve increases as pregnancy progresses.
Suri
Daesman
D
Thyroid Study Unit, Department of Medicine, University of Chicago, 5841 South Maryland Avenue, Mail Code 3090, Chicago, IL 60637, USA.
Moran
Jill
J
Hibbard
Judith U
JU
Kasza
Kristen
K
Weiss
Roy E
RE
D000311:Adrenal Glands / Q000502:physiology*; D000324:Adrenocorticotropic Hormone; D000328:Adult; D000450:Aldosterone / Q000097:blood*; D002352:Carrier Proteins / Q000032:analysis; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis* / Q000097:blood*; D011247:Pregnancy / Q000502:physiology*; D011446:Prospective Studies; D012463:Saliva / Q000737:chemistry*
D002352:Carrier Proteins; C027091:cortisol binding globulin; D000450:Aldosterone; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone