title: Differential expression of glutathione-S-transferase isoenzymes in various types of anemia in Taiwan.
abstract: BACKGROUND
  Published reports concerning the expression of GST in various anemias including aplastic, hemolytic, iron deficiency and thalassemia anemia has been insufficient. We improved the conventional GST assay by incorporating a chloroform treatment to remove the interference of hemoglobin and evaluated the altered expression of GSTs in various anemias in Taiwan.
  
  
  METHODS
  We incorporated a chloroform treatment to eliminate the interference of hemoglobin. Erythrocyte total GST and isoenzymes activities from 35 control subjects and 125 subjects of various anemias, including aplastic, hemolytic, iron deficiency and thalassemia anemias were measured spectrophotometrically.
  
  
  RESULTS
  Chloroform treatment did not significantly affect GST activities in erythrocytes of control subjects while the activities of erythrocyte total GST and alpha-GST were significantly increased in all anemic patients (P<0.001). The expression of mu-GST was significantly decreased, although at a less extent, in cases of aplastic, iron deficiency and thalassemia anemia (P<0.05), but pi-GST was not physiologically different in various types of anemia.
  
  
  CONCLUSIONS
  The determination of changes in erythrocyte GST activity is a promising indicator of oxidative stress conditions that occur in various types of anemia. Measurement of GST activity might be useful for the evaluation of prophylactic treatment in trials of antioxidant strategies.
authors.affiliation: School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Whei-Ling
authors.initials: WL
authors.lastname: Chiang
authors.forename: Yih-Shou
authors.initials: YS
authors.lastname: Hsieh
authors.forename: Shun-Fa
authors.initials: SF
authors.lastname: Yang
authors.forename: Tsang-An
authors.initials: TA
authors.lastname: Lu
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Chu
mesh_terms: D000328:Adult; D000740:Anemia / Q000097:blood* / Q000453:epidemiology; D016022:Case-Control Studies; D002725:Chloroform / Q000737:chemistry; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005982:Glutathione Transferase / Q000097:blood*; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D007527:Isoenzymes; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D013624:Taiwan / Q000453:epidemiology
chemical_list: D006454:Hemoglobins; D007527:Isoenzymes; D002725:Chloroform; D005982:Glutathione Transferase