title: Nucleolar apparatus in the macronucleus of Didinium nasutum (Ciliata): EM and 3D reconstruction.
abstract: The three-dimensional (3D) organization of nucleoli in the somatic nuclei (macronuclei) of recently fed and starved Didinium nasutum was reconstructed on the basis of serial ultra-thin sections. It was shown that nucleoli, looking on the single sections like individual separate structures, appeared to be parts of the large complicated branchy nucleolar networks. A 30 h starvation did not lead to disintegration of this network, but stimulated formation of numerous vacuoles in the granular component of nucleoli, which becomes more condensed. Unlike starved D. nasutum, in fed ciliates numerous holes appeared in the fibrillar component located at the periphery of nucleoli. These holes may presumably serve as channels for transporting newly synthesized rRNA. To our knowledge, this is the first report of a 3D reconstruction of the nucleolar apparatus in ciliates.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 119991 Moscow, Russia.
authors.forename: Olga G
authors.initials: OG
authors.lastname: Leonova
authors.forename: Bella P
authors.initials: BP
authors.lastname: Karajan
authors.forename: Yuri F
authors.initials: YF
authors.lastname: Ivlev
authors.forename: Julia L
authors.initials: JL
authors.lastname: Ivanova
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Popenko
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure*; D016798:Ciliophora / Q000235:genetics / Q000648:ultrastructure*; D004275:DNA, Ribosomal / Q000235:genetics; D021621:Imaging, Three-Dimensional; D008854:Microscopy, Electron; D009697:Nucleolus Organizer Region / Q000648:ultrastructure
chemical_list: D004275:DNA, Ribosomal