title: Micronuclei frequency in lymphocytes and antioxidants in the blood of traditional limited-resource farm workers exposed to pesticides.
abstract: Chronic low-level exposure to synthetic pesticides is implicated in many health conditions that result from the induction of oxidative stress, including cytogenetic damage. The objective of this study was to assess the risk of genotoxicity using micronuclei (MN) formation in lymphocytes and to determine changes in blood antioxidants superoxide dismutase (SOD) in erythrocytes (E) and glutathione (GSH) in E and plasma (PL) in farm workers for six months during a growing season. Blood and urine samples were collected once a month for six months (June to November 2003) from farm workers (n = 15) and urban unexposed controls (n = 10). Lymphocytes from blood were separated by density gradient centrifugation using Histopaque and cultured using the standard technique. There was no significant difference in the cytokinesis blocked proliferation index (CBPI) of lymphocytes between the farm workers and the control group, but there was a 76% increase in average MN frequency in lymphocytes of the farm worker group (P <or= 0.05). In addition, MN frequency peaked during August as compared to the other months and the controls (P <or= 0.05). An 18% decline was observed in the activity of E-SOD in the farm worker group (P <or= 0.05). GSH in E and PL were similar in both groups. These data suggest that the farm workers may be at a greater risk of developing genotoxicity due to continued exposure to pesticides, especially during the intensive growing season.
authors.affiliation: Nutrition and Health Program, Kentucky State University, Frankfort, Kentucky 40601, USA. avinash.tope@kysu.edu
authors.forename: Avinash
authors.initials: A
authors.lastname: Tope
authors.forename: Frederick N
authors.initials: FN
authors.lastname: Bebe
authors.forename: Myna
authors.initials: M
authors.lastname: Panemangalore
mesh_terms: D000975:Antioxidants / Q000032:analysis; D016022:Case-Control Studies; D018556:Crops, Agricultural; D004249:DNA Damage / Q000187:drug effects; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D005978:Glutathione / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects; D010575:Pesticides / Q000009:adverse effects*; D012307:Risk Factors; D012621:Seasons; D013482:Superoxide Dismutase / Q000032:analysis / Q000097:blood / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D010575:Pesticides; D013482:Superoxide Dismutase; D005978:Glutathione