title: Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.
abstract: BACKGROUND
  Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features.
  
  
  METHODS
  Cancer cells, isolated from pleural effusion using a gradient method, were cultivated.
  
  
  RESULTS
  Amylase production from the newly established cell line was confirmed by positive staining for alpha-amylase and increased amylase levels in the culture supernatant. Electron microscopy revealed zymogen granule-like structures. Sialylation of salivary-type amylase was confirmed directly from the cell line by examining the neuraminidase sensitivity and amylase elution profile under high-performance liquid chromatography. Neither EGFR or KRAS mutation were found.
  
  
  CONCLUSIONS
  This cell line offers a useful tool for analyzing the pathogenesis and pathophysiology of amylase-producing lung cancers. Moreover, it might be useful for probing the metastasis and invasiveness of lung cancer cells and for developing an early diagnostic method based on sialylated salivary-amylase production.
authors.affiliation: First Department of Medicine, Hokkaido University School of Medicine, North 15, West 7, Kita-ku, Sapporo, Japan. yokouchi@med.hokudai.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokouchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Asahina
authors.forename: Masahiko
authors.initials: M
authors.lastname: Shigemura
authors.forename: Takanori
authors.initials: T
authors.lastname: Moriyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Takaoka
authors.forename: Jun
authors.initials: J
authors.lastname: Moriya
authors.forename: Tomoo
authors.initials: T
authors.lastname: Itoh
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kinoshita
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Dosaka-Akita
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tsutsumi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Nishimura
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D000368:Aged; D000681:Amylases / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D012469:Salivary Glands / Q000201:enzymology
chemical_list: D000681:Amylases