Correlation between the expression level of c-maf and glutathione peroxidase-3 in c-maf -/- mice kidney and c-maf overexpressed renal tubular cells.
Large mafs are transcriptional factors and members of the basic leucine zipper (b-Zip) superfamily. Since we previously identified expression of c-maf in mouse kidney, we presently investigated the mRNA expression profile in the kidney of c-maf gene knockout mice by using DNA microarray, and plasma glutathione peroxidase-3 (GPx3) was predominantly downregulated. We focused on the relation between the expression level of c-maf and GPx3 in vivo and in vitro. Since GPx3 is an antioxidant enzyme, oxidative stress was induced by exposing a culture cell derived from mouse renal tubules (mIMCD3) to hydrogen peroxide. Real-time PCR demonstrated that mRNA expression of both c-maf and GPx3 increased in parallel during exposure to oxidative stress in a time- and dose-dependent manner. Then, the mIMCD3 cells were transfected with c-maf-cDNA containing plasmid, which resulted in an increase in mRNA and protein expression of GPx3 compared with the control cells. Thus, c-maf may be transcriptional regulator of GPx3 expression and modulate the antioxidative pathway in the kidney.
Shirota
S
S
Department of Medicine IV, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Yoshida
T
T
Sakai
M
M
Kim
J I
JI
Sugiura
H
H
Oishi
T
T
Nitta
K
K
Tsuchiya
K
K
D000818:Animals; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D005979:Glutathione Peroxidase / Q000096:biosynthesis*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D018384:Oxidative Stress / Q000502:physiology; D051257:Proto-Oncogene Proteins c-maf / Q000096:biosynthesis*
C495608:Maf protein, mouse; D051257:Proto-Oncogene Proteins c-maf; D006861:Hydrogen Peroxide; C116888:Gpx3 protein, mouse; D005979:Glutathione Peroxidase