Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues.
Dietary conjugated linoleic acid (CLA) affects fat deposition and lipid metabolism in mammals, including livestock. To determine CLA effects in Atlantic salmon (Salmo salar), a major farmed fish species, fish were fed for 12 weeks on diets containing fish oil or fish oil with 2% and 4% CLA supplementation. Fatty acid composition of the tissues showed deposition of CLA with accumulation being 2 to 3 fold higher in muscle than in liver. CLA had no effect on feed conversion efficiency or growth of the fish but there was a decreased lipid content and increased protein content after 4% CLA feeding. Thus, the protein:lipid ratio in whole fish was increased in fish fed 4% CLA and triacylglycerol in liver was decreased. Liver beta-oxidation was increased whilst both red muscle beta-oxidation capacity and CPT1 activity was decreased by dietary CLA. Liver highly unsaturated fatty acid (HUFA) biosynthetic capacity was increased and the relative proportion of liver HUFA was marginally increased in salmon fed CLA. CLA had no effect on fatty acid Delta6 desaturase mRNA expression, but fatty acid elongase mRNA was increased in liver and intestine. In addition, the relative compositions of unsaturated and monounsaturated fatty acids changed after CLA feeding. CLA had no effect on PPARalpha or PPARgamma expression in liver or intestine, although PPARbeta2A expression was reduced in liver at 4% CLA feeding. CLA did not affect hepatic malic enzyme activity. Thus, overall, the effect of dietary CLA was to increase beta-oxidation in liver, to reduce levels of total body lipid and liver triacylglycerol, and to affect liver fatty acid composition, with increased elongase expression and HUFA biosynthetic capacity.
Leaver
Michael J
MJ
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK. m.j.leaver@stir.ac.uk
Tocher
Douglas R
DR
Obach
Alex
A
Jensen
Linda
L
Henderson
R James
RJ
Porter
Allan R
AR
Krey
Grigorios
G
D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000096:biosynthesis; D004041:Dietary Fats / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D015870:Gene Expression / Q000187:drug effects; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D050602:Linoleoyl-CoA Desaturase / Q000096:biosynthesis; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000737:chemistry; D008291:Malate Dehydrogenase / Q000096:biosynthesis; D009132:Muscles / Q000737:chemistry; D047492:Peroxisome Proliferator-Activated Receptors / Q000096:biosynthesis; D020317:Salmo salar / Q000254:growth & development / Q000378:metabolism*
D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D044243:Linoleic Acids, Conjugated; D047492:Peroxisome Proliferator-Activated Receptors; D008291:Malate Dehydrogenase; D050602:Linoleoyl-CoA Desaturase; D002334:Carnitine O-Palmitoyltransferase