BCNU-loaded PEG-PLLA ultrafine fibers and their in vitro antitumor activity against Glioma C6 cells.
The purpose of the present study was to develop implantable BCNU-loaded poly(ethylene glycol)-poly(L-lactic acid) (PEG-PLLA) diblock copolymer fibers for the controlled release of 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU). BCNU was well incorporated and dispersed uniformly in biodegradable PEG-PLLA fibers by using electrospinning method. Environmental Scanning Electron Microscope (ESEM) images indicated that the BCNU-loaded PEG-PLLA fibers looked uniform and their surfaces were reasonably smooth. Their average diameters were below 1500 nm. The release rate of BCNU from the fiber mats increased with the increase of BCNU loading amount. In vitro cytotoxicity assay showed that the PEG-PLLA fibers themselves did not affect the growth of rat Glioma C6 cells. Antitumor activity of the BCNU-loaded fibers against the cells was kept over the whole experiment process, while that of pristine BCNU disappeared within 48 h. These results strongly suggest that the BCNU/PEG-PLLA fibers have an effect of controlled release of BCNU and are suitable for postoperative chemotherapy of cancers.
Xu
Xiuling
X
State key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Chen
Xuesi
X
Xu
Xiaoyi
X
Lu
Tiancheng
T
Wang
Xin
X
Yang
Lixin
L
Jing
Xiabin
X
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage*; D002330:Carmustine / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers; D004355:Drug Stability; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D019344:Lactic Acid / Q000008:administration & dosage*; D011091:Polyesters; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D011108:Polymers / Q000008:administration & dosage*; D051381:Rats; D012995:Solubility
D018906:Antineoplastic Agents, Alkylating; D004337:Drug Carriers; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D002330:Carmustine