Enantiomeric resolution of bifonazole by supercritical fluid chromatography.
The enantiomeric separation of bifonazole by supercritical fluid chromatography on Chiralpak AD has been studied. The effect of different modifiers (methanol, ethanol, 2-propanol, and acetonitrile) was examined. Enantioseparation was possible with all of them, but the best results were provided by the alcohol-type ones. The resolution was higher than 5 in all cases. The isoelution temperatures Tiso were calculated from the study of the temperature effect for the different organic modifiers. The value of Tiso was below the working temperature range on using methanol, but above it with ethanol or 2-propanol.
Toribio
Laura
L
Department of Analytical Chemistry, Faculty of Science, University of Valladolid, Valladolid, Spain. ltoribio@qa.uva.es
del Nozal
Ma Jesús
MJ
Bernal
José L
JL
Alonso
Cristina
C
Jiménez
Juan J
JJ
D000935:Antifungal Agents / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry*; D015394:Molecular Structure; D013237:Stereoisomerism; D013696:Temperature
D000935:Antifungal Agents; D007093:Imidazoles; C036596:bifonazole