Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes.
Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.
Gorr
Ingo H
IH
Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
Reis
Alexandra
A
Boos
Dominik
D
Wühr
Martin
M
Madgwick
Suzanne
S
Jones
Keith T
KT
Stemmann
Olaf
O
D000818:Animals; D016203:CDC2 Protein Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006657:Histones / Q000378:metabolism; D006801:Humans; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D064249:Securin; D064247:Separase; D014981:Xenopus
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D006657:Histones; D009363:Neoplasm Proteins; D064249:Securin; C578002:pituitary tumor-transforming protein 1, human; D016203:CDC2 Protein Kinase; D010450:Endopeptidases; C577987:ESPL1 protein, human; D064247:Separase