title: The C-type Arabidopsis thioredoxin reductase ANTR-C acts as an electron donor to 2-Cys peroxiredoxins in chloroplasts.
abstract: 2-Cys peroxiredoxins (Prxs) play important roles in the antioxidative defense systems of plant chloroplasts. In order to determine the interaction partner for these proteins in Arabidopsis, we used a yeast two-hybrid screening procedure with a C175S-mutant of Arabidopsis 2-Cys Prx-A as bait. A cDNA encoding an NADPH-dependent thioredoxin reductase (NTR) isotype C was identified and designated ANTR-C. We demonstrated that this protein effected efficient transfer of electrons from NADPH to the 2-Cys Prxs of chloroplasts. Interaction between 2-Cys Prx-A and ANTR-C was confirmed by a pull-down experiment. ANTR-C contained N-terminal TR and C-terminal Trx domains. It exhibited both TR and Trx activities and co-localized with 2-Cys Prx-A in chloroplasts. These results suggest that ANTR-C functions as an electron donor for plastidial 2-Cys Prxs and represents the NADPH-dependent TR/Trx system in chloroplasts.
authors.affiliation: Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jeong Chan
authors.initials: JC
authors.lastname: Moon
authors.forename: Ho Hee
authors.initials: HH
authors.lastname: Jang
authors.forename: Ho Byoung
authors.initials: HB
authors.lastname: Chae
authors.forename: Jung Ro
authors.initials: JR
authors.lastname: Lee
authors.forename: Sun Yong
authors.initials: SY
authors.lastname: Lee
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Jung
authors.forename: Mi Rim
authors.initials: MR
authors.lastname: Shin
authors.forename: Hye Song
authors.initials: HS
authors.lastname: Lim
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Chung
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
authors.forename: Kyun Oh
authors.initials: KO
authors.lastname: Lee
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
mesh_terms: D017360:Arabidopsis / Q000201:enzymology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology; D004583:Electrons; D009249:NADP / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D054464:Peroxiredoxins; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D009249:NADP; D010544:Peroxidases; C493631:2-Cys peroxiredoxin A, Arabidopsis; D054464:Peroxiredoxins; C514013:ANTR-C protein, Arabidopsis; D013880:Thioredoxin-Disulfide Reductase