title: DNA integrity assay: a plasma-based screening tool for the detection of prostate cancer.
abstract: OBJECTIVE
  The aim of this study was to evaluate the utility of the DNA integrity assay (DIA) as a plasma-based screening tool for the detection of prostate cancer.
  
  
  METHODS
  Blood samples were collected from patients with biopsy-proven prostate cancer prior to prostatectomy (n = 123) and processed as two-spin plasma preparations. The three control groups included: males <40 years old with no history of cancer (group 1, n = 20); cancer-free postprostatectomy patients (group 2, n = 25), and patients with a negative prostate biopsy (group 3, n = 22). DNA in plasma preparations were isolated, hybrid-captured, and DNA fragments (200 bp, 1.3, 1.8, and 2.4 kb) were multiplexed in real-time PCR. A baseline cutoff was determined for individual fragment lengths to establish a DIA score for each patient sample.
  
  
  RESULTS
  Patients with prostate cancer (86 of 123; 69.9%) were determined to have a positive DIA score of >or=7. The DIA results from control groups 1, 2, and 3 showed specificities of 90%, 92%, and 68.2%, respectively. Of the patients with negative age-adjusted prostate-specific antigen (PSA) and prostate cancer, 19 of 30 (63%) had a positive DIA score. The area under the receiver operating characteristic curve for DIA was 0.788.
  
  
  CONCLUSIONS
  While detecting 69.9% of those with prostate cancer, DIA maintained an overall specificity of 68.2% to 92%, a range favorably comparable to that currently accepted for PSA (60-70%). The variability in specificity between control groups is likely explained by the established 19% to 30% detection of prostate cancer on subsequent biopsies associated with control group 3. DIA detected 63% of the prostate cancers undetected by currently accepted PSA ranges.
authors.affiliation: Robert E. Wise Research and Education Institute, Cell and Molecular Biology Laboratory, Lahey Clinic, Burlington, MA 01805, USA..
authors.forename: Robert
authors.initials: R
authors.lastname: Hanley
authors.forename: Kimberly M
authors.initials: KM
authors.lastname: Rieger-Christ
authors.forename: David
authors.initials: D
authors.lastname: Canes
authors.forename: Norah R
authors.initials: NR
authors.lastname: Emara
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Shuber
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Boynton
authors.forename: John A
authors.initials: JA
authors.lastname: Libertino
authors.forename: Ian C
authors.initials: IC
authors.lastname: Summerhayes
mesh_terms: D000328:Adult; D004273:DNA, Neoplasm / Q000097:blood* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004273:DNA, Neoplasm