title: Mutation rate and pattern of microsatellites in common carp (Cyprinus carpio L.).
abstract: Microsatellites are popular molecular markers in genetic and evolutionary studies. Their mutational dynamics have been extensively studied in humans and fruit flies, but few data were available in fish. By genotyping 55 individuals of a F1 pedigree, we investigated the mutation rates and patterns of 49 microsatellites in one of the most important fresh water fish species, the common carp (Cyprinus carpio L.). The overall mutation rate of the 49 loci was 5.56 x 10(-4)/locus/generation (95% confidence interval 1.52 x 10(-4) and 1.63 x 10(-3)). The change of allele size was between +2 to -5 repeat units, assuming that the mutation allele arose from the parental allele most similar in size to the mutant.
authors.affiliation: Molecular Population Genetics Group, Temasek Life Sciences Laboratory, Singapore, Singapore. genhua@tll.org.sg
authors.forename: G H
authors.initials: GH
authors.lastname: Yue
authors.forename: L
authors.initials: L
authors.lastname: David
authors.forename: L
authors.initials: L
authors.lastname: Orban
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics*; D019143:Evolution, Molecular; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics; D008957:Models, Genetic; D009154:Mutation / Q000235:genetics*; D017422:Sequence Analysis, DNA