Diterpenes from "Pini Resina" and their preferential cytotoxic activity under nutrient-deprived condition.
In the course of our search for anticancer agents based on a novel anti-austerity strategy, we found that the 70 % EtOH extract of "Pini Resina" showed 100 % preferential cytotoxicity at the concentration of 50 microg/mL. Further bioassay-guided fractionation and purification led to the isolation of 15 compounds including one new compound 7-oxo-13 alpha-hydroxyabiet-8(14)-en-18-oic acid (1). Their structures were elucidated on the basis of spectroscopic analysis. Among the isolated compounds, methyl abieta-8,11,13-trien-18-oate (7) showed the most potent preferential cytotoxicity at 10 microg/mL under nutrient-deprived condition.
Zaidi
Syed Faisal
SF
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Awale
Suresh
S
Kalauni
Surya Kant
SK
Tezuka
Yasuhiro
Y
Esumi
Hiroyasu
H
Kadota
Shigetoshi
S
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D045744:Cell Line, Tumor; D005591:Chemical Fractionation; D003470:Culture Media; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D006801:Humans; D028223:Pinus / Q000737:chemistry*; D012116:Resins, Plant / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*
D000972:Antineoplastic Agents, Phytogenic; D003470:Culture Media; D004224:Diterpenes; D012116:Resins, Plant; C013893:rosin