Characterization of the Drosophila melanogaster ribosomal proteome.
We have combined high-resolution two-dimensional (2-D) gel electrophoresis with mass spectrometry to identifying proteins represented in a 2-D gel database of Drosophila melanogaster ribosomes. First, we purified ribosomes from third instar Drosophila larvae and constructed a high-resolution 2-D gel database containing 58 Coomassie blue stained polypeptides. Next, we carried out preparative 2-D PAGE to isolate some of the polypeptides and characterize them by MALDI-TOF. Using this strategy we identified 52 ribosomal spots in the database, and in each case confirmed their identity by MALDI-TOF/TOF. The database can be used to analyze Minute mutants of Drosophila.
Alonso
Jana
J
Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Universidad Autónoma, Cantoblanco, 28049-Madrid, Spain.
Santarén
Juan F
JF
D000595:Amino Acid Sequence; D000818:Animals; D030562:Databases, Protein; D029721:Drosophila Proteins / Q000032:analysis* / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D007814:Larva / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000235:genetics; D020543:Proteome / Q000032:analysis*; D012269:Ribosomal Proteins / Q000032:analysis* / Q000235:genetics; D012270:Ribosomes / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D029721:Drosophila Proteins; D010455:Peptides; D020543:Proteome; D012269:Ribosomal Proteins