Absorption of orthotopically transplanted intestine in rats: evaluation of amino acid absorption.
During the early phase of our intestinal transplant program, a low-lipid, amino acid-based feeding was employed for most recipients. However, relatively little is known regarding the amino acid absorption by a graft following intestinal transplantation. Sixty Wistar rats were randomly divided into two groups (n = 30). The animals received two-step orthotopic intestine transplantation (OIT), or the control; enterectomy with anastomoses. We measured mucosal Na(+)/K(+)-ATPase and intestinal absorption using stable isotope techniques in vivo. Compared with controls, mucosal Na(+)/K(+)-ATPase activity in OIT decreased by 38.3% and 38.4% at 2 and 4 weeks, and returned to baseline and control group values at 8 weeks. Glycine absorption decreased at 2 weeks and returned to baseline at 4 weeks in OIT compared with that in controls. These results suggested that absorption as assessed by mucosal Na(+)/K(+)-ATPase and glycine, returned to baseline at least 4 weeks after transplantation.
Li
Y
Y
Department of Surgery, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, China. liys@medmail.com.cn
Zhu
L
L
Li
J
J
D000596:Amino Acids / Q000378:metabolism*; D000714:Anastomosis, Surgical; D000818:Animals; D005998:Glycine / Q000378:metabolism*; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000637:transplantation*; D007583:Jejunum / Q000033:anatomy & histology / Q000166:cytology; D008297:Male; D023421:Models, Animal; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D000596:Amino Acids; D000254:Sodium-Potassium-Exchanging ATPase; D005998:Glycine