Effects of pulsed electric fields on DNA of human lymphocytes.
The effects of pulsed electric fields of low frequency (50 Hz) on DNA of human lymphocytes were investigated. The influence of additional external factors, such as hydrogen peroxide (H2O2) and gamma-irradiation, as well as the repair efficiency in these lymphocytes, was also evaluated. The comet assay, a very sensitive and rapid method for detecting DNA damage at the single cells level was the method used. A significant amount of damage was observed after exposure to the electric fields, compared to the controls. After 2 h incubation at 37 degrees C, a proportion of damage was repaired. H2O2 and gamma-irradiation increased the damage to lymphocytes exposed to pulsed electric fields according to the dose used, while the amount of the repair was proportional to the damage.
Delimaris
J
J
DNA Repair Laboratory, Institute of Biology, National Center for Scientific Research Demokritos, Athens, Greece.
Tsilimigaki
S
S
Messini-Nicolaki
N
N
Ziros
E
E
Piperakis
S M
SM
D000328:Adult; D020552:Comet Assay / Q000379:methods; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D016877:Oxidants / Q000009:adverse effects
D016877:Oxidants; D004247:DNA; D006861:Hydrogen Peroxide