[Toxic effect of butenolide on chondrocyte differentiation and the protective effect of selenium].
OBJECTIVE
To study the effect of butenolide (BUT) on cultured chondrocytes differentiation and the possible protective effects of selenium (Se).


METHODS
Ex-vivo cultured chondrocytes were divided into six groups: (1) Control group (without BUT and Se); (2) Se 0.1 microg/ml control group; (3) BUT 0.1 microg/ml group; (4) BUT 1.0 microg/ml group; (5) BUT 5.0 microg/ml group; and (6) BUT 1.0 microg/ml + Se 0.1 microg/ml group. The expression of collagen II (Col II), collagen X (ColX), basic fibroblast growth factor (bFGF), and parathyroid hormone-related peptide (PTHrP) in (or around) chondrocytes in all groups were analyzed by immunohistochemistry.


RESULTS
The expressions of Col II in 1.0 microg/ml BUT group and 5.0 microg/ml BUT group were significantly lower than those in the control group (P < 0.05). The expression of Col II in 1.0 microg/ml BUT + Se group were significantly higher than those in the 1.0 microg/ml BUT group and 5.0 microg/ml BUT group (P < 0.05). The expressions of bFGF and PTHrP of BUT groups were significantly higher than those in the Se and control groups (P < 0.05). No expression of ColX was observed in all groups.


CONCLUSIONS
BUT can affect the collagen II synthesis of the chondrocytes. Selenium supplementation may play a protective role.
Zuo
Hong
H
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850.
Guo
Xiong
X
Wang
Shi-Jie
SJ
Shi
Zhong-Li
ZL
Peng
Shuang-Qing
SQ
Cao
Jun-Ling
JL
Zhang
Zeng-Tie
ZT
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D006801:Humans; D020011:Protective Agents / Q000494:pharmacology; D012643:Selenium / Q000494:pharmacology*; D013605:T-2 Toxin / Q000633:toxicity*
D020011:Protective Agents; C004511:butenolide; D012643:Selenium; D013605:T-2 Toxin; D015107:4-Butyrolactone