title: A genomewide screen for components of the RNAi pathway in Drosophila cultured cells.
abstract: Posttranscriptional silencing by RNAi is initiated by dsRNAs that are processed into siRNAs that ultimately target homologous mRNAs for degradation. We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway in Drosophila melanogaster. The screen identified seven genes that affect the RNAi response, five with previously described function (AGO2, Tis11, Hsc70-3, Hsc70-4, and hdc) and two annotated genes (CG17265 and CG10883).
authors.affiliation: Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Silke
authors.initials: S
authors.lastname: Dorner
authors.forename: Lawrence
authors.initials: L
authors.lastname: Lum
authors.forename: Michelle
authors.initials: M
authors.lastname: Kim
authors.forename: Renato
authors.initials: R
authors.lastname: Paro
authors.forename: Philip A
authors.initials: PA
authors.lastname: Beachy
authors.forename: Rachel
authors.initials: R
authors.lastname: Green
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004331:Drosophila melanogaster; D020868:Gene Silencing; D017344:Genes, Insect; D005821:Genetic Techniques; D016678:Genome; D008156:Luciferases / Q000378:metabolism; D008957:Models, Genetic; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D012330:RNA, Double-Stranded / Q000737:chemistry; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D008156:Luciferases