title: Reduced bronchial CD4+ T-cell density in smokers with occupational asthma.
abstract: Cigarette smoking may alter bronchial inflammation in asthma. Multicolour immunohistofluorescent examination on bronchial cryosections was used to examine bronchial inflammatory cell infiltrate in patients with occupational asthma. Monoclonal antibodies to CD3, CD4, CD8, T-cell receptor-delta1, CD68 and human leukocyte antigen-DR were combined to identify T-cell subsets and macrophages in bronchial biopsies from 20 workers with occupational asthma (12 smokers and eight nonsmokers), 15 healthy workers (seven smokers and eight nonsmokers) and 10 nonsmoking, nonexposed controls. The increased subepithelial CD4+ T-cell density in nonsmoking asthmatics was not present in smoking asthmatics, who had the lowest CD4+ T-cell density of all groups. The decreased subepithelial CD4+ and CD8+ T-cell density correlated with a reduction in lung function, as measured by percentage predicted forced expiratory volume in one second, in smoking asthmatics only. Although smoking asthmatics had a significantly increased number of intraepithelial CD8+ T-cells and macrophages compared with nonsmoking asthmatics, the proportion of gammadelta-T-cells was significantly decreased in both asthmatic groups. Smoking asthmatics had a distinctly different distribution of T-cell subsets compared with nonsmoking asthmatics. The accumulation of subepithelial CD4+ T-cells, which was observed in nonsmoking asthmatics, appeared to be inhibited in smoking asthmatics, suggesting a smoking-induced bronchial immune modulation, at least in occupational asthma in the aluminium industry.
authors.affiliation: Laboratory for Mucosal Immunology (LMI), Dept of Oral Biology, University of Oslo, Oslo, Norway. tonebs@odont.uio.no
authors.forename: T
authors.initials: T
authors.lastname: Sjåheim
authors.forename: J
authors.initials: J
authors.lastname: Kongerud
authors.forename: O
authors.initials: O
authors.lastname: Bjørtuft
authors.forename: P A
authors.initials: PA
authors.lastname: Drabløs
authors.forename: D
authors.initials: D
authors.lastname: Malterud
authors.forename: T S
authors.initials: TS
authors.lastname: Halstensen
mesh_terms: D000395:Air Pollutants, Occupational / Q000009:adverse effects; D000535:Aluminum; D001249:Asthma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D001706:Biopsy; D001999:Bronchoscopy; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D016022:Case-Control Studies; D005541:Forced Expiratory Volume / Q000502:physiology; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007958:Leukocyte Count; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology; D009784:Occupational Diseases / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016273:Occupational Exposure; D012907:Smoking / Q000009:adverse effects* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*
chemical_list: D000395:Air Pollutants, Occupational; D006684:HLA-DR Antigens; D000535:Aluminum