title: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: A new matrix of 3,4-diaminobenzophenone (DABP) was demonstrated to be advantageous in the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. With DABP as a matrix, intact oligonucleotide ions can be readily produced with lower laser powers, resulting in better detection limits, less fragmentation and fewer alkali metal ion adducts compared with the results obtained with conventional matrices. Importantly, minimal fragmentation and fewer alkali metal ion adducts were seen even at low concentrations of oligonucleotides. It was also found that samples prepared with DABP are highly homogenous and therefore reducing the need for finding 'sweet' spots in MALDI. In addition, excellent shot-to-shot reproducibility, resolution and signal-to-noise ratio were seen with DABP as the matrix.
authors.affiliation: National Chromatographic R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fu
authors.forename: Songyun
authors.initials: S
authors.lastname: Xu
authors.forename: Chensong
authors.initials: C
authors.lastname: Pan
authors.forename: Mingliang
authors.initials: M
authors.lastname: Ye
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
authors.forename: Baochuan
authors.initials: B
authors.lastname: Guo
mesh_terms: D001577:Benzophenones / Q000737:chemistry*; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry; D010655:Phenylenediamines / Q000737:chemistry*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: C512423:3,4-diaminobenzophenone; D001577:Benzophenones; D009841:Oligonucleotides; D010655:Phenylenediamines