Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule.
In this study, we investigated the effect of the microenvironment provided by alginate-poly-L-lysine-alginate (APA) microcapsule with liquefied or gelled core on the proliferation, viability, and metabolism of human cells, including anchorage-dependent MCF-7 breast cancer cells and primary fibroblasts, and anchorage-independent K-562 leukemia cells; cells in conventional culture were used as control. The growth pattern of cells in microcapsule was examined by phase-contrast micrography. The cell viability, proliferation, organization, and gene expression were evaluated by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, hematoxylin and eosin staining, live/dead staining, 5-bromo-20-deoxyuridine labeling, and immunohistochemistry, respectively. Cell metabolism was determined by measuring glucose and lactate concentrations in medium. The results demonstrate that APA microcapsule with liquefied core provides a microenvironment for both anchorage-dependent and anchorage-independent cells to grow into a large cell aggregate and maintain cell viability at a constant level for a period of time. In conclusion, cells in APA microcapsule are alive and have proliferation potential with lower metabolism rate. APA microcapsule may be a useful tool for in vitro tumor cell modeling and anticancer drug screening as well as for cancer gene therapy. In addition, it lays a solid foundation for the use of microencapsulation in cell culture in vitro and cell implantation in vivo.
Zhang
Xulang
X
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Wang
Wei
W
Xie
Yubing
Y
Zhang
Ying
Y
Wang
Xiuli
X
Guo
Xin
X
Ma
Xiaojun
X
D000464:Alginates; D001672:Biocompatible Materials; D001709:Biotechnology; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005782:Gels; D005947:Glucose / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D019344:Lactic Acid / Q000378:metabolism; D008863:Microspheres; D009363:Neoplasm Proteins / Q000378:metabolism; D011107:Polylysine / Q000031:analogs & derivatives
D000464:Alginates; D001672:Biocompatible Materials; D005782:Gels; D009363:Neoplasm Proteins; C051546:alginate-polylysine-alginate; D011107:Polylysine; D019344:Lactic Acid; D005947:Glucose