Short-sequence-repeat analysis of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium isolates collected from animals throughout the United States reveals both stability of loci and extensive diversity.
We analyzed the multilocus short sequence repeats (SSRs) of 211 and 56 isolates of Mycobacterium avium subsp. paratuberculosis and M. avium subsp. avium, respectively. The M. avium subsp. paratuberculosis isolates could be differentiated into 61 genotypes. The M. avium subsp. avium isolates showed limited diversity. These SSRs are stable and suitable for studying the molecular epidemiology of M. avium subsp. paratuberculosis.
Harris
N Beth
NB
National Veterinary Services Laboratories, Ames, IA 50010, USA.
Payeur
Janet B
JB
Kapur
Vivek
V
Sreevatsan
Srinand
S
D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D009162:Mycobacterium avium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010283:Paratuberculosis / Q000382:microbiology*; D012091:Repetitive Sequences, Nucleic Acid; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary*; D014481:United States
D004269:DNA, Bacterial