[Analysis of the local synthesis of Toxoplasma gondii-specific immunoglobulins in the anterior eye chamber fluid. Report of two cases].
We reported two cases strongly suspected of ocular toxoplasmosis, in which a puncture of anterior eye chamber was performed for a final verification of clinical diagnosis. In both patients, fresh focal lesions of retinochoroiditis associated with old pigmented retinal scars were diagnosed. The 62-year-old women had a serological evidence of recently acquired T. gondii infection with an increasing IgG titre of a low avidity and a presence of specific IgM and IgA antibodies. In the 13-year-old girl, an immunological profile of the chronic infection was shown, confirmed by a high IgG avidity value. In the patient with a clinical picture of recurrent congenital toxoplasmosis, intraocular production of T. gondii-specific IgG was found in anterior eye chamber. The analysis of the specific antibodies synthesis in ocular fluid by the Western blot seems to be a valuable immunodiagnostic method for a final diagnosis of ocular toxoplasmosis.
Paul
M
M
Klinika Chorób Pasozytniczych i Tropikalnych AM, 60-355 Poznań, ul. Przybyszewskiego 49.
Stefaniak
J
J
Pecold
K
K
Twardosz-Pawlik
H
H
Kacprzak
E
E
Rychlicki
W
W
D000293:Adolescent; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D001082:Aqueous Humor / Q000276:immunology / Q000469:parasitology*; D002825:Chorioretinitis / Q000175:diagnosis* / Q000469:parasitology; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D008875:Middle Aged; D012008:Recurrence; D012698:Serologic Tests; D014122:Toxoplasma / Q000302:isolation & purification; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000469:parasitology; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis* / Q000469:parasitology
D000913:Antibodies, Protozoan; D007136:Immunoglobulins