title: Chondrocyte gene expression under applied surface motion.
abstract: A cartilage bioreactor has been designed that is intended to approximate the kinematics of natural joints and allows for functional cartilage tissue engineering studies. In particular, interface motion can be generated by oscillation of a ball over the surface of a construct. The present study investigated the specific effect of applied articular motion on the gene expression of chondrocytes cultured in 3D scaffolds, with a particular emphasis on different superficial zone protein (SZP)/lubricin transcripts. Cylindrical porous polyurethane scaffolds were seeded with bovine articular chondrocytes and subjected to dynamic compression, with or without articulation against a ceramic hip ball. Articular motion markedly up-regulated the mRNA expression of the four previously described and two newly identified SZP/lubricin isoforms and of cartilage oligomeric matrix protein (COMP), and, to a lesser extent, aggrecan, type II collagen and TIMPs, while axial compression alone had no effect on the chondrocytes' gene expression levels. These results demonstrate the beneficial effect of articular motion not only for stimulation of important lubricating molecules, but also for the preservation of the chondrocytic phenotype.
authors.affiliation: Biomaterials and Tissue Engineering Program, AO Research Institute, Davos, Switzerland. sibylle.grad@aofoundation.org
authors.forename: Sibylle
authors.initials: S
authors.lastname: Grad
authors.forename: Cynthia R
authors.initials: CR
authors.lastname: Lee
authors.forename: Markus A
authors.initials: MA
authors.lastname: Wimmer
authors.forename: Mauro
authors.initials: M
authors.lastname: Alini
mesh_terms: D000818:Animals; D019149:Bioreactors; D002358:Cartilage, Articular / Q000166:cytology* / Q000502:physiology; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D019902:Chondrocytes / Q000378:metabolism* / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D009068:Movement / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011509:Proteoglycans / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013314:Stress, Mechanical; D023822:Tissue Engineering; D015854:Up-Regulation; D000099158:Proteoglycan 4
chemical_list: D006023:Glycoproteins; D011509:Proteoglycans; D012333:RNA, Messenger; D000099158:Proteoglycan 4; C498836:PRG4 protein, Bos taurus