Identification of a novel Cys2/His2-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco.
In a previous work, we identified a Cys(2)/His(2)-type zinc-finger transcription repressor, (ZFT1), that functions in a spermine-mediated signal transduction pathway in tobacco plants. Database search disclosed the presence of another Cys(2)/His(2)-type zinc-finger protein ZFP1 (accession number AAC06243) in tobacco plants. In this work, we characterized ZFP1 and investigated whether this protein is also involved in a Spm-signaling pathway. This factor showed the highest identity to petunia ZPT2-2 and higher similarity to petunia ZPT2-3, Arabidopsis STZ/ZAT10, soybean SCOF-1, red pepper CAZFP1/CaPIF1 as well as to tobacco ZFT1. ZFP1 localized to the nucleus and had a specific DNA-binding activity, supportive to be a transcription factor. Furthermore, the protein had a mild repression activity on transcription in plant cells. The expression of ZFP1, encoding ZFP1, was upregulated during tobacco mosaic virus-induced hypersensitive response. ZFP1 expression was also induced by exogenously applied spermine and its induction was repressed by inhibitors of amine oxidase/polyamine oxidase. Collectively, our data indicate that ZFP1 is a new transcription factor which functions in a spermine-signaling pathway in tobacco.
Mitsuya
Yoshiko
Y
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi 980-8577, Japan.
Takahashi
Yoshihiro
Y
Uehara
Yukiko
Y
Berberich
Thomas
T
Miyazaki
Atsushi
A
Takahashi
Hideki
H
Kusano
Tomonobu
T
D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000032:analysis; D006146:Guanidines / Q000494:pharmacology; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000037:antagonists & inhibitors; D010802:Phylogeny; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D011073:Polyamines / Q000378:metabolism; D011700:Putrescine / Q000031:analogs & derivatives / Q000494:pharmacology; D016415:Sequence Alignment; D015398:Signal Transduction; D013096:Spermine / Q000378:metabolism* / Q000494:pharmacology; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000502:physiology; D014157:Transcription Factors / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D015854:Up-Regulation; D016335:Zinc Fingers
D006146:Guanidines; D010940:Plant Proteins; D011073:Polyamines; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; C044445:MDL 72527; D013096:Spermine; D010088:Oxidoreductases; C004479:pimagedine; D011700:Putrescine