Direct association between caspase 3 and alpha5beta1 integrin and its role during anoikis of rat fibroblasts.
We have demonstrated a direct association between alpha5beta1 integrin and caspase 3, both pro- and mature enzyme, in various sub-cellular compartments of rat fibroblasts undergoing anoikis. Integrin associated caspase 3 showed high activity in the plasma membranes, whereas in the cytosol and microsomal fraction it showed little or no activity. Our results suggest a possible role for recycled un-ligated alpha5beta1 integrin molecules between cytosol and plasma membrane, in regulation of caspase-3 activity and induction of cell death in adhesion-deprived cells.
Rajeswari
J
J
Centre for Cellular and Molecular Biology, Cell Biology Department, Habsiguda, Uppal Road, Hyderabad, Andhra Pradesh 500 007, India.
Pande
Gopal
G
D000818:Animals; D023102:Anoikis; D053148:Caspase 3 / Q000378:metabolism / Q000502:physiology*; D002448:Cell Adhesion; D002451:Cell Compartmentation; D002460:Cell Line; D053938:DNA Fragmentation; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D039081:Integrin alpha5beta1 / Q000378:metabolism / Q000502:physiology*; D018613:Microscopy, Confocal; D051381:Rats
D039081:Integrin alpha5beta1; D053148:Caspase 3