Siro(haem)amide in Allochromatium vinosum and relevance of DsrL and DsrN, a homolog of cobyrinic acid a,c-diamide synthase, for sulphur oxidation.
In the purple sulphur bacterium Allochromatium vinosum, the prosthetic group of dissimilatory sulphite reductase (DsrAB) was identified as siroamide, an amidated form of the classical sirohaem. The genes dsrAB are the first two of a large cluster of genes necessary for the oxidation of sulphur globules stored intracellularly during growth on sulphide and thiosulphate. DsrN is homologous to cobyrinic acid a,c diamide synthase and may therefore catalyze glutamine-dependent amidation of sirohaem. Indeed, an A. vinosumDeltadsrN in frame deletion mutant showed a significantly reduced sulphur oxidation rate that was fully restored upon complementation with dsrN in trans. Sulphite reductase was still present in the DeltadsrN mutant. DsrL is a homolog of the small subunits of bacterial glutamate synthases and was proposed to deliver glutamine for sirohaem amidation. However, recombinant DsrL does not exhibit glutamate synthase activity nor does the gene complement a glutamate synthase-deficient Escherichia coli strain. Deletion of dsrL showed that the encoded protein is absolutely essential for sulphur oxidation in A. vinosum.
Lübbe
Yvonne J
YJ
Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Germany.
Youn
Hyung-Sun
HS
Timkovich
Russell
R
Dahl
Christiane
C
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002841:Chromatiaceae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D017353:Gene Deletion; D005816:Genetic Complementation Test; D005970:Glutamate Synthase / Q000235:genetics / Q000378:metabolism*; D006418:Heme / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013455:Sulfur / Q000378:metabolism*
D001426:Bacterial Proteins; D011994:Recombinant Proteins; C093014:siroamide; D006418:Heme; D013455:Sulfur; D005970:Glutamate Synthase; D009247:NADH, NADPH Oxidoreductases; C078110:cob(II)yrinic acid a,c-diamide reductase; D050862:Oxidoreductases Acting on Sulfur Group Donors