Flow cytometric analysis of micronuclei in peripheral blood reticulocytes: I. Intra- and interlaboratory comparison with microscopic scoring.
Accumulating evidence suggests that reticulocytes (RETs) in the peripheral blood of rats may represent a suitable cell population for use in the micronucleus assay, despite the ability of the rat spleen to selectively remove micronucleated erythrocytes from the peripheral circulation. To evaluate the analytical performance of a previously described flow cytometric method (Torous et al., 2003, Toxicol. Sci. 74, 309-314) that may allow this assay to be conducted using peripheral blood in lieu of bone marrow sampling, we compared the sensitivity and performance characteristics of the flow cytometric technique with two established microscopy-based scoring methods. Peripheral blood samples from single Sprague-Dawley rats treated for 6 days with either vehicle or cyclophosphamide were prepared in replicate for scoring by the three methods at different laboratories. These blood-based measurements were compared to those derived from bone marrow specimens from the same animals, stained with acridine orange, and scored by microscopy. Through the analysis of replicate specimens, inter- and intralaboratory variability were evaluated for each method. Scoring reproducibility over time was also evaluated. These data support the premise that rat RETs harvested from peripheral blood are a suitable cell population to assess genotoxicant-induced micronucleus formation. The interlaboratory comparison provides evidence of the general robustness of the micronucleus endpoint using different analytical approaches. Furthermore, data presented herein demonstrate a clear advantage of flow cytometry-based scoring over microscopy-significantly lower inter- and intralaboratory variation and higher statistical sensitivity.
Dertinger
Stephen D
SD
Litron Laboratories, Rochester, New York 14623, USA.
Bishop
Michelle E
ME
McNamee
James P
JP
Hayashi
Makoto
M
Suzuki
Takayoshi
T
Asano
Norihide
N
Nakajima
Madoka
M
Saito
Junichiro
J
Moore
Martha
M
Torous
Dorothea K
DK
Macgregor
James T
JT
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D019411:Clinical Laboratory Techniques / Q000592:standards; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005453:Fluorescence; D007441:Intubation, Gastrointestinal; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests / Q000379:methods / Q000592:standards; D008856:Microscopy, Fluorescence / Q000379:methods*; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity; D011419:Propidium / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012156:Reticulocytes / Q000187:drug effects* / Q000378:metabolism
D009153:Mutagens; D011419:Propidium; D003520:Cyclophosphamide; D016650:Fluorescein-5-isothiocyanate