Decellularized placental matrices for adipose tissue engineering.
A tissue-engineered adipose substitute would be invaluable to plastic surgeons for reconstructive, corrective, and cosmetic procedures. This work involves the design of a scaffold for soft tissue augmentation incorporating the decellularized extracellular matrix (ECM) of human placenta. We have developed a protocol to decellularize an intact, large segment (8 cm by 8 cm) of the human placenta. To facilitate the complete decellularization of the dense matrix, a system was designed to perfuse the required chemicals into the placenta via the existing vasculature. Following processing, the original architecture of the placental ECM was preserved, including an intact vascular network. Histological, immunohistochemical, and scanning electron microscopic analyses confirmed the removal of the cells and cellular debris and characterized the composition and structure of the matrix. In vitro cell culture experimentation showed that the placental decellular matrix (PDM) could facilitate the adhesion of primary human adipose precursor cells at early time points. The PDM has great potential for use as a scaffold for adipose tissue engineering, as the placenta is a rich source of human ECM components that can be readily harvested without harm to the donor.
Flynn
Lauren
L
Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario, Canada, M5S 3E5.
Semple
John L
JL
Woodhouse
Kimberly A
KA
D000273:Adipose Tissue / Q000378:metabolism* / Q000473:pathology; D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007511:Ischemia / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D010477:Perfusion; D010920:Placenta / Q000378:metabolism* / Q000648:ultrastructure; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials