Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex.
We are the first to have isolated a protein (186 amino acid residues) encoded by the open reading frame adjacent to the end of the BspD6I nickase (N.BspD6I) gene. Cleavage of both DNA strands near the sequence recognized by nickase (5 -GAGTC/5 -GACTC) occurs when this protein is added to the reaction mixture containing N.BspD6I. The protein encoded by the open reading frame and the nickase are suggested to be subunits of heterodimeric restriction endonuclease R.BspD6I.
Yunusova
A K
AK
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
Rogulin
E A
EA
Artyukh
R I
RI
Zheleznaya
L A
LA
Matvienko
N I
NI
D000595:Amino Acid Sequence; D004262:DNA Restriction Enzymes / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000235:genetics* / Q000378:metabolism; D019281:Dimerization; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D021122:Protein Subunits / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D029968:Escherichia coli Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; C514554:BspD6I protein, E coli