Anthracene based base-discriminating fluorescent oligonucleotide probes for SNPs typing: synthesis and photophysical properties.
2- and 9-Anthracenecarboxamide labeled 2'-deoxyuridines were synthesized and their photophysical properties were examined. These oligonucleonucleotide probes are capable of detecting adenine base on a target DNA sequence. It was also found that 2-anthracene based oligonucleotide probe is more efficient than the corresponding 9-anthracene based oligonucleotide in the application for DNA chip based SNP detection, due to its longer emission wavelength and high fluorescence intensity.
Saito
Yoshio
Y
Department of Materials Chemistry and Engineering, School of Engineering, Nihon University, Japan. saitoy@chem.ce.nihon-u
Motegi
Kaori
K
Bag
Subhendu Sekhar
SS
Saito
Isao
I
D000225:Adenine; D000873:Anthracenes; D002264:Carboxylic Acids; D004252:DNA Mutational Analysis / Q000379:methods*; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013057:Spectrum Analysis
C476641:2-anthracenecarboxylate; D000873:Anthracenes; D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; C010030:9-anthroic acid; C034020:anthracene; D000225:Adenine