Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.
BACKGROUND
Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features.


METHODS
Cancer cells, isolated from pleural effusion using a gradient method, were cultivated.


RESULTS
Amylase production from the newly established cell line was confirmed by positive staining for alpha-amylase and increased amylase levels in the culture supernatant. Electron microscopy revealed zymogen granule-like structures. Sialylation of salivary-type amylase was confirmed directly from the cell line by examining the neuraminidase sensitivity and amylase elution profile under high-performance liquid chromatography. Neither EGFR or KRAS mutation were found.


CONCLUSIONS
This cell line offers a useful tool for analyzing the pathogenesis and pathophysiology of amylase-producing lung cancers. Moreover, it might be useful for probing the metastasis and invasiveness of lung cancer cells and for developing an early diagnostic method based on sialylated salivary-amylase production.
Yokouchi
Hiroshi
H
First Department of Medicine, Hokkaido University School of Medicine, North 15, West 7, Kita-ku, Sapporo, Japan. yokouchi@med.hokudai.ac.jp
Yamazaki
Koichi
K
Asahina
Hajime
H
Shigemura
Masahiko
M
Moriyama
Takanori
T
Takaoka
Kazuo
K
Moriya
Jun
J
Itoh
Tomoo
T
Kinoshita
Ichiro
I
Dosaka-Akita
Hirotoshi
H
Tsutsumi
Yutaka
Y
Nishimura
Masaharu
M
D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D000368:Aged; D000681:Amylases / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D012469:Salivary Glands / Q000201:enzymology
D000681:Amylases