Selective detection and transport of fully matched DNA by DNA-loaded microtubule and kinesin motor protein.
DNA-loaded microtubules (MTs) moving on a kinesin motor protein-coated substrate can selectively hybridize with a target fully matched DNA over single-base mismatched DNA and transport it. This technique is capable of collecting target biomolecules toward one point site to design new methodology of DNA analysis.
Taira
Shu
S
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, AIST, Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan.
Du
Yong-Zhong
YZ
Hiratsuka
Yuichi
Y
Konishi
Kaoru
K
Kubo
Tai
T
Uyeda
Taro Q P
TQ
Yumoto
Noboru
N
Kodaka
Masato
M
D000327:Adsorption; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry*; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification*; D015342:DNA Probes; D016547:Kinesins / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000737:chemistry*; D013552:Swine
D001426:Bacterial Proteins; D015342:DNA Probes; D008869:Microtubule-Associated Proteins; C066114:biotin-streptavidin complex; D001710:Biotin; D004247:DNA; D016547:Kinesins