rHsp90 gene expression in response to several environmental stresses in rice (Oryza sativa L.).
In this study, the gene for a rice (Oryza sativa L.) 90 kDa heat shock protein (rHsp90, GenBank accession no. AB037681) was identified by screening rice root cDNAs that were up-regulated under carbonate (NaHCO(3)) stress using the method of differential display, and cloned. The open-reading-frame of rHsp90-cDNA was predicted to encode a protein containing 810 amino acids, which showed high similarity to proteins in Hordeum vulgare (accession no. X67960) and Catharathus roseus (accession no. L14594). Further studies showed that rHsp90 mRNA accumulated following exposure to several abiotic stresses, including salts (NaCl, NaHCO(3) and Na(2)CO(3)), desiccation (using polyethylene glycol), high pH (8.0 and 11.0) and high temperature (42 and 50 degrees C). Yeast (Saccharomyces cerevisiae) over-expressing rHsp90 exhibited greater tolerance to NaCl, Na(2)CO(3) and NaHCO(3) and tobacco seedlings over-expressing rHsp90 could tolerate salt concentrations as high as 200 mM NaCl, whereas untransformed control seedlings couldn't. These results suggest that rHsp90 plays an important role in multiple environmental stresses.
Liu
Dali
D
Alkali Soil Natural Environmental Science Center (ASNESC), Stress Molecular Biology Laboratory, Northeast Forestry University, Harbin 150040, PR China.
Zhang
Xinxin
X
Cheng
Yuxiang
Y
Takano
Tetsuo
T
Liu
Shenkui
S
D000595:Amino Acid Sequence; D002254:Carbonates / Q000494:pharmacology; D003001:Cloning, Molecular; D003890:Desiccation; D018506:Gene Expression Regulation, Plant; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D017693:Sodium Bicarbonate / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism
D002254:Carbonates; D018841:HSP90 Heat-Shock Proteins; D010940:Plant Proteins; D012965:Sodium Chloride; C005686:sodium carbonate; D017693:Sodium Bicarbonate