From 'omes to biology.
Technologies that have emerged from the genome project have dramatically increased our ability to generate data on the way in which organisms respond to their environments, how they execute their programmes of development and growth, and how these are altered in the development of disease states. However, our ability to analyse these large datasets has not kept pace with our ability to generate them and consequently new strategies must be developed to address the issues associated with their analysis. One approach that we have employed quite successfully is to look at data from microarrays (or proteomics or metabolomics experiments) not as independent datasets, but rather as elements of a much larger body of biological information across various scales that must be integrated with, and interpreted within, the context of such ancillary data. Here we outline the general approach and provide three examples from published studies of the way in which we have applied this strategy.
Quackenbush
J
J
Dana-Farber Cancer Institute and Harvard School of Public Health, Boston, MA 02115, USA. johnq@jimmy.harvard.edu
D000818:Animals; D002874:Chromosome Mapping; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D016678:Genome; D023281:Genomics / Q000379:methods*; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D049490:Systems Biology