Inhibition of hcv replication in HCV replicon by shRNAs.
We show that the vector-derived long dsRNA specifically inhibits the replication of HCV RNA in HCV replicon. We designed a long dsRNA targeted to the full-length HCV IRES/core elements (1-to 377-nt). Our results revealed that the replication of HCV RNA was reduced to near background levels in a sequence-specific manner by the long dsRNAs in the HCV replicon. We also designed four shRNAs against several regions (120- to 139-nt, 260- to 279-nt, 330- to 349-nt, and 340- to 359-nt) of the HCV IRES/Core elements. The two HCV IRES/core-specific shRNAs, 330- to 349-nt and 340- to 359-nt, containing the AUG initiation codon sequence showed stronger HCV inhibitory effects than the other two shRNAs, 120- to 139-nt and 260- to 279-nt.
Hamazaki
Hiroyuki
H
Department of Life and Environmental Sciences, Chiba Institute of Technology, Chiba, Japan.
Takahashi
Hitoshi
H
Shimotohno
Kunitada
K
Miyano-Kurosaki
Naoko
N
Takaku
Hiroshi
H
D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D012330:RNA, Double-Stranded / Q000138:chemical synthesis / Q000494:pharmacology*; D012093:Replicon / Q000187:drug effects* / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects*
D012330:RNA, Double-Stranded