title: Proteomic response of Schizosaccharomyces pombe to static and oscillating extremely low-frequency electromagnetic fields.
abstract: There is considerable public concern regarding the health effects of exposure to low-frequency electromagnetic fields. In addition, the association between exposure and disease incidence or the possible biological effects of exposure are unclear. Using 2D-DIGE and MS in a blind study, we have investigated the effects of static and oscillating extremely low-frequency electromagnetic fields (ELF EMFs) on the proteomes of wild type Schizosaccharomyces pombe and a Sty1p deletion mutant which displays increased sensitivity to a variety of cellular stresses. Whilst this study identifies a number of protein isoforms that display significant differential expression across experimental conditions, there was no correlation between their patterns of expression and the ELF EMF exposure regimen. We conclude that there are no significant effects of either static or oscillating EMF on the yeast proteome at the sensitivity afforded by 2D-DIGE. We hypothesise that the proteins identified must be sensitive to subtle changes in culture and/or handling conditions, and that the identification of these proteins in other proteomic studies should be treated with some caution when the results of such studies are interpreted in a biological context.
authors.affiliation: Ludwig Institute for Cancer Research, Department of Biochemistry and Molecular Biology University College London, UK.
authors.forename: John
authors.initials: J
authors.lastname: Sinclair
authors.forename: Mark
authors.initials: M
authors.lastname: Weeks
authors.forename: Amna
authors.initials: A
authors.lastname: Butt
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Worthington
authors.forename: Akunna
authors.initials: A
authors.lastname: Akpan
authors.forename: Nic
authors.initials: N
authors.lastname: Jones
authors.forename: Mike
authors.initials: M
authors.lastname: Waterfield
authors.forename: Donald
authors.initials: D
authors.lastname: Allanand
authors.forename: John F
authors.initials: JF
authors.lastname: Timms
mesh_terms: D004574:Electromagnetic Fields; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D040901:Proteomics; D012568:Schizosaccharomyces / Q000378:metabolism* / Q000528:radiation effects*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*; D055672:Static Electricity
chemical_list: D029702:Schizosaccharomyces pombe Proteins