title: Demonstration of a new biosensing concept for immunodiagnostic applications based on change in surface conductance of antibodies after biomolecular interactions.
abstract: We report an important observation that the surface conductivity of antibody layer immobilized on polylysine-coated glass substrate decreases upon the formation of complex with their specific antigens. This change in conductivity has been observed for both monoclonal and polyclonal antibodies. The conductance of monoclonal mouse IgG immobilized on polylysine-coated glass substrate changed from 1.02x10(-8) ohm(-1) to 1.41x10(-11) ohm(-1) at 10 V when complex is formed due to the specific biomolecular interactions with rabbit anti-mouse IgG F(ab')(2). Similar behavior was observed when the same set up was tested in two clinical assays: (1) anti-Leishmania antigen polyclonal antibodies taken from Kala Azar positive patient serum interacting with Leishmania promastigote antigen, and (2) anti-p21 polyclonal antibodies interacting with p21 antigen. The proposed concept can represent a new immunodiagnostic technique and may have wide ranging applications in biosensors and nanobiotechnology too.
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Vashist
authors.forename: Inderpreet
authors.initials: I
authors.lastname: Kaur
authors.forename: Ram Prakash
authors.initials: RP
authors.lastname: Bajpai
authors.forename: Lalit Mohan
authors.initials: LM
authors.lastname: Bharadwaj
authors.forename: Rupinder
authors.initials: R
authors.lastname: Tewari
authors.forename: Roberto
authors.initials: R
authors.lastname: Raiteri
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D015374:Biosensing Techniques; D004553:Electric Conductivity; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011817:Rabbits
chemical_list: D000936:Antigen-Antibody Complex