Cyclosporine inhibits endotoxin-induced vasodilation of isolated rat resistance arterioles.
BACKGROUND
An isolated arteriole fails to dilate in response to endotoxin unless a segment of aorta is included in the perfusion system. The unknown substance released by the aorta after exposure to endotoxin is dependent upon the NF-kappaB pathway and induces inducible nitric oxide synthase (iNOS) in the arteriole. The purpose of this study was to determine if cyclosporine A (CSA) that inhibits both NF-kappaB and iNOS would prevent the vasodilatory response to endotoxin.


METHODS
Rats were injected with either 10 mg/kg of CSA or oil vehicle followed by the removal of a cremaster muscle. The feeding arteriole was isolated from the cremaster and mounted on micropipettes and pressurized to 70 mmHg in a superfused tissue bath. After an hour equilibration to develop spontaneous tone, a 1 cm segment of aorta was placed in the superfusion system upstream from the arteriole and Salmonella enteriditis endotoxin was added to the buffer at a concentration of 2.5 microg/mL (ET) or continued infusion of buffer alone. Internal diameters of cannulated arterioles were measured with videomicroscopy and videocalipers for an additional hour.


RESULTS
Arterioles downstream from an aorta exposed to vehicle but not endotoxin developed 22.8 +/- 3.7% tone that remained unchanged over the following hour. Arterioles exposed to endotoxin started with 22.5 +/- 2.8% spontaneous tone and this fell over the following hour to 11.8 +/- 3.6%, P < 0.05. Pre-treatment of the rats with CSA tended to increase resting tone and completely prevented the loss of tone after endotoxin.


CONCLUSIONS
Pre-treatment of the aortic segment with CSA resulted in the development of increased tone in the downstream arteriole and completely blocked the vasodilatory response to endotoxin. These results suggest that CSA or a similar compound may be useful in the treatment of septic shock.
Obias
Vincent J
VJ
Department of Surgery, Eastern Virginia Medical School, Norfolk, Virginia 23507-1912, USA.
Rushing
Gregory D
GD
Prewitt
Russell L
RL
Rice
Darian
D
Britt
L D
LD
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D001160:Arterioles / Q000187:drug effects / Q000502:physiology; D016572:Cyclosporine / Q000494:pharmacology*; D019446:Endotoxemia / Q000188:drug therapy* / Q000503:physiopathology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000188:drug therapy* / Q000503:physiopathology; D014655:Vascular Resistance; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology
D007166:Immunosuppressive Agents; D016328:NF-kappa B; D016572:Cyclosporine; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat