title: A novel fluorescence imaging technique combining deconvolution microscopy and spectral analysis for quantitative detection of opportunistic pathogens.
abstract: A novel fluorescence imaging technique based on deconvolution microscopy and spectral analysis is presented here as an alternative to confocal laser scanning microscopy. It allowed rapid, specific and simultaneous identification of five major opportunistic pathogens, relevant for public health, in suspension and provided quantitative results.
authors.affiliation: Florida Gulf Coast University, Fort Myers, FL, USA. mlepuil@fgcu.edu <mlepuil@fgcu.edu>
authors.forename: M
authors.initials: M
authors.lastname: Le Puil
authors.forename: J P
authors.initials: JP
authors.lastname: Biggerstaff
authors.forename: B L
authors.initials: BL
authors.lastname: Weidow
authors.forename: J R
authors.initials: JR
authors.lastname: Price
authors.forename: S A
authors.initials: SA
authors.lastname: Naser
authors.forename: D C
authors.initials: DC
authors.lastname: White
authors.forename: R S
authors.initials: RS
authors.lastname: Alberte
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016360:Clostridioides difficile / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008856:Microscopy, Fluorescence; D015269:Mycobacterium avium Complex / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D013050:Spectrometry, Fluorescence
chemical_list: D012336:RNA, Ribosomal, 16S