Tissue microarray analysis of FAS, Bcl-2, Bcl-x, ER, PgR, Hsp60, p53 and Her2-neu in breast carcinoma.
BACKGROUND
The aim of this study was to detect immunohistochemical markers in breast carcinoma by means of tissue microarray analysis (TMA) and to associate their expressions with clinicopathological features and prognosis. Fatty acid synthase, bcl-2, bcl-x, p53, estrogen and progesterone receptors, heat shock protein 60 and Her2-neu (c-erbB-2) were evaluated in a group of 149 breast carcinoma patients with a 5-year follow-up period.


METHODS
TMA blocks were made by using duplicate 0.6-mm diameter tissue cores from each paraffin block.


RESULTS
Statistical analysis revealed that tumor stage (p=0.003) and node status (p=0.001) were the only two prognostic markers of disease-free survival. Moreover, FAS and bcl-x showed an independent effect on recurrence (p=0.005). The node status was the only marker of overall survival (p=0.05).


CONCLUSIONS
Our data confirmed recent reports associating the stage of disease, FAS and Bcl-x expressions with recurrence and outcome. These data demonstrated that TMA is an effective substitute for conventional histochemical-immunohistochemical techniques.
Sebastiani
Valeria
V
Department of Experimental Medicine and Pathology, School of Specialization in Anatomic Pathology, University of Rome La Sapienza, Rome, Italy.
Botti
Claudio
C
Di Tondo
Ugo
U
Visca
Paolo
P
Pizzuti
Laura
L
Santeusanio
Giuseppe
G
Alo
Piero Luigi
PL
D001943:Breast Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D018834:Chaperonin 60 / Q000032:analysis / Q000096:biosynthesis; D064429:Fatty Acid Synthases / Q000032:analysis / Q000096:biosynthesis; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis*; D040081:Protein Array Analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000096:biosynthesis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000032:analysis / Q000096:biosynthesis; D011980:Receptors, Progesterone / Q000032:analysis / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis; D051020:bcl-X Protein / Q000032:analysis / Q000096:biosynthesis
D018834:Chaperonin 60; D064429:Fatty Acid Synthases; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D016159:Tumor Suppressor Protein p53; D051020:bcl-X Protein; C495901:TP53 protein, human