Multiple processing of Ig-Hepta/GPR116, a G protein-coupled receptor with immunoglobulin (Ig)-like repeats, and generation of EGF2-like fragment.
Ig-Hepta/GPR116 is a member of the LNB-TM7 subfamily of G protein-coupled receptors (GPCRs), also termed the adhesion GPCRs, whose members have EGF, cadherin, lectin, thrombospondin, or Ig repeats in their long N-terminus. In this study, we established that Ig-Hepta is processed at multiple sites yielding the following four fragments: (i) presequence (amino acid residues 1-24), (ii) proEGF2 (25-223, alpha-fragment), (iii) Ig repeats (224-993, beta-chain), and (iv) TM7 (994-1349, gamma-chain). The proEGF2 region is converted to EGF2 (52-223) by the processing enzyme furin and remains attached to the beta- and gamma-chains. Expression of some mRNA species was affected by the presence of alpha-fragment. These results suggest that the furin-processed alpha-fragment is involved in cellular signaling.
Fukuzawa
Taku
T
Department of Biological Sciences, Tokyo Institute of Technology, 4259-B19, Nagatsuta-cho, Midori-ku, Yokohama 226-8501.
Hirose
Shigehisa
S
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D017931:DNA Primers; D045683:Furin / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D047468:Immunoprecipitation; D046228:Microarray Analysis; D024101:Mitochondrial Proteins / Q000378:metabolism*; D020653:Peptide Elongation Factor G / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
C516038:ADGRF5 protein, human; D017931:DNA Primers; C441681:GFM2 protein, human; D024101:Mitochondrial Proteins; D020653:Peptide Elongation Factor G; D043562:Receptors, G-Protein-Coupled; D045683:Furin