Muscle regeneration by adipose tissue-derived adult stem cells attached to injectable PLGA spheres.
The [corrected] use of adult stem cells for cell-based tissue engineering and regeneration strategies represents a promising approach for skeletal muscle repair. We have evaluated the combination of adipose tissue-derived adult stem cells (ADSCs) obtained from autologous liposuction and injectable poly(lactic-co-glycolic acid) (PLGA) spheres for muscle regeneration. ADSCs attached to PLGA spheres and PLGA spheres alone were cultured in myogenic medium for 21 days and injected subcutaneously into the necks of nude mice. After 30 and 60 days, the mice were sacrificed, and newly formed tissues were analyzed by immunostaining, H and E staining, and RT-PCR. We found that ADSCs attached to PLGA spheres, but not PLGA spheres alone, were able to generate muscle tissue. These findings suggest that ADSCs and PLGA spheres are useful materials for muscle tissue engineering and that their combination can be used in clinical settings for muscle regeneration.
Kim
MiJung
M
Asan Institute for Life Sciences, University of Ulsan, Seoul, Republic of Korea. mjkimhsc@korea.com
Choi
Yu Suk
YS
Yang
Seung Hye
SH
Hong
Hea-Nam
HN
Cho
Sung-Woo
SW
Cha
Sang Myun
SM
Pak
Jhang Ho
JH
Kim
Chan Wha
CW
Kwon
Seog Woon
SW
Park
Chan Jeoung
CJ
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D006801:Humans; D019344:Lactic Acid; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D009132:Muscles / Q000502:physiology*; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D012038:Regeneration; D033581:Stem Cell Transplantation / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*
D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid