Influence of ginsenoside Rb1 on brain neurosteroid during acute immobilization stress.
This study examined whether or not acute stress is linked to increases in the neurosteroid levels, which is a well-known neurotransmitters associated with stress stimuli. The ginsenoside, Rb1, was tested in order to better understand its potential effects on altering the neurosteroid levels and ultimately attenuating stress. The optimal stressed condition was checked by measuring the 5a-dihydroprogesterone (DHP) and allopregnanolone (THP) levels in the brain after immobilization stress at various times. Based on this result, an acute stress model was set up to give 30 min of immobilization stress. The DHP and THP brain levels of the stressed mice were then investigated after administering Rb1 orally (10 mg/kg). These results were compared with the neurosteroid level in the stressed mice not given Rbl. Saline was administered orally to the nonstressed mice to check the placebo effect. Acute immobilization stress induced an increase in the THP and DHP concentration in the frontal cortex and cerebellum. When Rb1 was administered orally prior to immobilization stress, the THP level in the frontal cortex and cerebellum was significantly lower than that in the stressed animals not given Rbl. On the other hand, the DHP level was lower in the cerebellum only. This suggests that the metabolism of the brain neurosteroids is linked to psychological stress, and Rb1 attenuates the stress-induced increase in neurosteroids.
Lee
Sang Hee
SH
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Cheongryang, Seoul, Korea.
Jung
Byung Hwa
BH
Choi
Sang Yoon
SY
Kim
Sun Yeou
SY
Lee
Eunjoo H
EH
Chung
Bong Chul
BC
D043582:5-alpha-Dihydroprogesterone / Q000378:metabolism*; D000818:Animals; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism; D036145:Ginsenosides / Q000494:pharmacology*; D007103:Immobilization; D051379:Mice; D008813:Mice, Inbred ICR; D023421:Models, Animal; D011280:Pregnanolone / Q000378:metabolism*; D013315:Stress, Psychological / Q000378:metabolism / Q000517:prevention & control*; D013997:Time Factors
D036145:Ginsenosides; C442759:ginsenoside Rb1; D043582:5-alpha-Dihydroprogesterone; D011280:Pregnanolone