Synthesis and enzymatic susceptibility of a series of novel GM2 analogs.
A series of GM2 analogs in which GM2 epitope was coupled to a variety of glycosyl lipids were designed and synthesized to investigate the mechanism of enzymatic hydrolysis of GM2 ganglioside. The coupling of N-Troc-protected sialic acid and p-methoxyphenyl galactoside acceptor gave the crystalline disaccharide, which was further coupled with galactosamine donor to give the desired GM2 epitope trisaccharide. After conversion into the corresponding glycosyl donor, the trisaccharide was coupled with galactose, glucose and artificial ceramide (B30) to give the final compounds. The result on hydrolysis of GM2 analogs indicates that GM2 activator protein requires one spacer sugar between GM2 epitope and the lipid moiety to assist the hydrolysis of the terminal GalNAc residue.
Fuse
Tomoaki
T
Department of Applied Bio-organic Chemistry, Gifu University, Gifu 501-1193, Japan.
Ando
Hiromune
H
Imamura
Akihiro
A
Sawada
Naoki
N
Ishida
Hideharu
H
Kiso
Makoto
M
Ando
Takayuki
T
Li
Su-Chen
SC
Li
Yu-Teh
YT
D004798:Enzymes / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D000939:Epitopes / Q000737:chemistry / Q000378:metabolism; D005678:G(M2) Ganglioside / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D013661:Tay-Sachs Disease / Q000201:enzymology / Q000378:metabolism
D004798:Enzymes; D000939:Epitopes; D005678:G(M2) Ganglioside