Immunomodulating effects of fractioned polysaccharides isolated from Yu-Ping-Feng-Powder in cyclophosphamide-treated mice.
Yu-Ping-Feng-Powder (YP), a traditional Chinese medicine prescription, is widely applied in China for the cure and prevention of diseases related to immunodeficiency. To test whether the fractioned polysaccharides (YPF-P) isolated from YP have immunomodulating activities, the effects of YPF-P on cyclophosphamide (Cy)-treated mice were studied in relation to phagocytosis of macrophage, splenocyte proliferation, and humoral, and cellular immunity parameters. It was found that YPF-P enhances phagocytic activity, augments ConA- or LPS-stimulated T cell proliferation, increases the quantitative haemolysis of SRBC (QHS) and delayed-type hypersensitivity reaction (DTH) to dinitrofluorobenzene. Hence, YPF-P restored the immuno-competence suppressed by Cy. YPF-P also augmented IL-2 and IFN-gamma production, but failed to increase IL-4 production, which indicates that there is high probability that it enhance Th1 function. These results suggested that YPF-P has immunomodulating effects and that the polysaccharides constitute one of the active components of YP.
Chen
Xiang-Tao
XT
School of Pharmacy, Anhui Medical University, Hefei, China. chenxiangtao69@hotmail.com
Li
Jun
J
Wang
Hui-Li
HL
Cheng
Wen-Ming
WM
Zhang
Lei
L
Ge
Jin-Fang
JF
D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000633:toxicity*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007111:Immunity, Cellular / Q000187:drug effects; D007155:Immunologic Factors / Q000008:administration & dosage / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000633:toxicity; D007274:Injections, Intraperitoneal; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D011208:Powders; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D008934:Mitogens; D011134:Polysaccharides; D011208:Powders; D015847:Interleukin-4; D007371:Interferon-gamma; D003520:Cyclophosphamide