title: Effect of hyperbaric oxygen on liver regeneration in a rat model.
abstract: Hyperbaric oxygen therapy is a treatment that has been gradually implemented for the treatment of several pathologic conditions. The present study evaluated the effect of hyperbaric oxygen therapy for hepatic regeneration and its relationship to mitochondrial function. Male Wistar rats underwent partial hepatectomy (70%) and subsequently underwent two sessions of hyperbaric oxygen (90 minutes each, at a pressure of 2 ATA). The animals were sacrificed at 24 and 48 hours after surgery. Hepatic regeneration was evaluated by the dry weight of the remaining liver, the hepatic regeneration rate, the hepatic DNA content, and the hepatocyte proliferation rate using the "proliferating cell nuclear antigen" (PCNA) content. Function of the mitochondria was evaluated by its oxygen consumption during respiratory states 3 and 4, its respiratory control ratio (RCR), its membrane potential, as well as its osmotic swelling. We also measured serum levels of aminotransferases. The results revealed an increased dry weight of the remaining liver, regeneration rate, and DNA content at 24 and 48 hours after hepatectomy. The hepatocyte proliferation rate was significantly higher among animals treated with hyperbaric oxygen therapy at 48 hours after surgery. There was no significant difference in aminotransferase levels. Mitochondrial respiration revealed reduced oxygen consumption in state 3 after 48 hours. These results demonstrated that hyperbaric oxygen stimulates hepatic regeneration at 24 and 48 hours after 70% hepatectomy. The effect of hyperbaric oxygen on hepatic tissue occurs without tissue damage and protects mitochondria after 48 hours.
authors.affiliation: Special Liver Transplantation Unit, Departments of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo, Avanida Bandeirantes 3.900, CEP 14049-900 Ribeirão Preto, São Paulo, Brazil.
authors.forename: E C
authors.initials: EC
authors.lastname: Tolentino
authors.forename: O
authors.initials: O
authors.lastname: Castro e Silva
authors.forename: S
authors.initials: S
authors.lastname: Zucoloto
authors.forename: M E J
authors.initials: ME
authors.lastname: Souza
authors.forename: M C J
authors.initials: MC
authors.lastname: Gomes
authors.forename: A K
authors.initials: AK
authors.lastname: Sankarankutty
authors.forename: G R
authors.initials: GR
authors.lastname: Oliveira
authors.forename: O
authors.initials: O
authors.lastname: Feres
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D006498:Hepatectomy / Q000379:methods; D006931:Hyperbaric Oxygenation; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D008930:Mitochondria, Liver / Q000502:physiology*; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000032:analysis
chemical_list: D012709:Serum Albumin; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin