Accumulation of oxidative damage during replicative aging of the yeast Saccharomyces cerevisiae.
Comparison of senescent yeast obtained by the "baby machine" technique with 2-day-old stationary phase cells revealed decreased activities of glutathione reductase, glutathione S-transferase, glutathione peroxidase and alcohol dehydrogenase, reduction of total antioxidant capacity, protein glycation and accumulation of products of oxidative damage: protein carbonyls and DNA damage assessed by augmented content of 8-oxoguanine and increased tail momentum of cellular DNA in the comet assay. These results are consistent with a role for oxidative damage during replicative senescence of Saccharomyces cerevisiae.
Grzelak
Agnieszka
A
Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland. agrzelak@biol.uni.lodz.pl
Macierzyńska
Ewa
E
Bartosz
Grzegorz
G
D000975:Antioxidants / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D020552:Comet Assay / Q000379:methods; D004249:DNA Damage; D004271:DNA, Fungal; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
D000975:Antioxidants; D004271:DNA, Fungal; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione