title: Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes.
abstract: Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.
authors.affiliation: Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Ingo H
authors.initials: IH
authors.lastname: Gorr
authors.forename: Alexandra
authors.initials: A
authors.lastname: Reis
authors.forename: Dominik
authors.initials: D
authors.lastname: Boos
authors.forename: Martin
authors.initials: M
authors.lastname: Wühr
authors.forename: Suzanne
authors.initials: S
authors.lastname: Madgwick
authors.forename: Keith T
authors.initials: KT
authors.lastname: Jones
authors.forename: Olaf
authors.initials: O
authors.lastname: Stemmann
mesh_terms: D000818:Animals; D016203:CDC2 Protein Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006657:Histones / Q000378:metabolism; D006801:Humans; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D064249:Securin; D064247:Separase; D014981:Xenopus
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D006657:Histones; D009363:Neoplasm Proteins; D064249:Securin; C578002:pituitary tumor-transforming protein 1, human; D016203:CDC2 Protein Kinase; D010450:Endopeptidases; C577987:ESPL1 protein, human; D064247:Separase