title: Adiponectin attenuates allergen-induced airway inflammation and hyperresponsiveness in mice.
abstract: BACKGROUND
  Epidemiologic data indicate an increased incidence of asthma in the obese.
  
  
  OBJECTIVE
  Because serum levels of the insulin-sensitizing and anti-inflammatory adipokine adiponectin are reduced in obese individuals, we sought to determine whether exogenous adiponectin can attenuate allergic airway responses.
  
  
  METHODS
  We sensitized and challenged BALB/cJ mice with ovalbumin (OVA). Alzet micro-osmotic pumps were implanted in the mice to deliver continuous infusions of buffer or adiponectin (1.0 microg/g/d), which resulted in an approximate 60% increase in serum adiponectin levels. Two days later, mice were challenged with aerosolized saline or OVA once per day for 3 days. Mice were examined 24 hours after the last challenge.
  
  
  RESULTS
  OVA challenge increased airway responsiveness to intravenous methacholine, bronchoalveolar lavage fluid cells, and T(H)2 cytokine levels. Importantly, each of these responses to OVA was reduced in adiponectin- versus buffer-treated mice. OVA challenge caused a 30% reduction in serum adiponectin levels and a corresponding decrease in adipose tissue adiponectin mRNA expression. OVA challenge also decreased pulmonary mRNA expression of each of 3 proposed adiponectin-binding proteins, adiponectin receptor 1, adiponectin receptor 2, and T-cadherin.
  
  
  CONCLUSIONS
  Our results indicate that serum adiponectin is reduced during pulmonary allergic reactions and that adiponectin attenuates allergic airway inflammation and airway hyperresponsiveness in mice.
  
  
  CONCLUSIONS
  The data suggest that adiponectin might play a role in the relationship between obesity and asthma.
authors.affiliation: Physiology Program, Department of Environmental Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA. sshore@hsph.harvard.edu
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Shore
authors.forename: Raya D
authors.initials: RD
authors.lastname: Terry
authors.forename: Lesley
authors.initials: L
authors.lastname: Flynt
authors.forename: Aimin
authors.initials: A
authors.lastname: Xu
authors.forename: Christopher
authors.initials: C
authors.lastname: Hug
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000235:genetics / Q000494:pharmacology; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000485:Allergens / Q000494:pharmacology; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000276:immunology / Q000517:prevention & control*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007073:Immunoglobulin E / Q000097:blood; D007962:Leukocytes / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000494:pharmacology; D011014:Pneumonia / Q000276:immunology / Q000517:prevention & control*; D012333:RNA, Messenger / Q000096:biosynthesis; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D000485:Allergens; D015820:Cadherins; C099972:H-cadherin; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C475419:adiponectin receptor 1, mouse; C475421:adiponectin receptor 2, mouse; D007073:Immunoglobulin E; D010047:Ovalbumin