Selective sensing of metalloproteins from nonselective binding using a fluorogenic amphiphilic polymer.
Nonconjugated fluorogenic amphiphilic polymers containing an anthracene chromophore exhibit fluorescence quenching in the presence of metalloproteins, although the binding of the polymer to proteins is not selective. The reason for this difference is that the possible conformational changes that protein binding could bring about on a polymer do not affect the fluorescence properties of a pendent chromophore in nonconjugated polymers. This is in contrast to the nonspecific binding and response found with conjugated polymers to proteins.
Sandanaraj
Britto S
BS
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Demont
Robert
R
Aathimanikandan
Sivakumar V
SV
Savariar
Elamprakash N
EN
Thayumanavan
S
S
D005456:Fluorescent Dyes / Q000737:chemistry*; D008667:Metalloproteins / Q000737:chemistry*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*
D005456:Fluorescent Dyes; D008667:Metalloproteins; D011108:Polymers; D013501:Surface-Active Agents