Increased expression of stress proteins in the surf clam Donax variabilis following hydrogen sulfide exposure.
Endogenous free radical production and resulting oxidative damage may result from exposure to hypoxia, hyperoxia, or hydrogen sulfide. Previous investigations of sulfide-induced oxidative damage have produced conflicting results, perhaps because these studies utilized species presumably adapted to sulfide. We examined the effects of sulfide, hypoxia and hyperoxia on the surf clam Donax variabilis to test whether these stressors induce a cellular response to oxidative stress. These clams inhabit high-energy sandy beaches and are unlikely to have specific adaptations to these stressors. In duplicate flow-through experiments performed in fall and spring, clams were exposed to normoxia (22 kPa P(O(2))), hypoxia (10 kPa), hyperoxia (37 kPa), or sulfide with normoxia ( approximately 100 mumol L(-1), 22 kPa respectively) for 24 h. We quantified whole-animal expression of three antioxidants (Cu/Zn and Mn superoxide dismutases, glutathione peroxidase), a lipid peroxidation marker (4-hydroxy-2E-nonenol-adducted protein), a DNA repair enzyme (OGG1-m), four heat shock proteins (small Hsp, Hsp60, Hsp70, and mitochondrial Hsp70), ubiquitin, and actin. Clams exposed to sulfide showed upregulation of the greatest number of stress proteins and the pattern was consistent with a cellular response to oxidative stress. Furthermore, there was a marked seasonality, with greater stress protein expression in clams from the spring.
Joyner-Matos
Joanna
J
Department of Zoology, University of Florida. Gainesville, FL 32611, USA.
Downs
Craig A
CA
Julian
David
D
D000199:Actins / Q000096:biosynthesis; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D049872:Bivalvia / Q000187:drug effects / Q000378:metabolism*; D045647:DNA Glycosylases / Q000096:biosynthesis; D005979:Glutathione Peroxidase / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D018496:Hyperoxia / Q000503:physiopathology; D000860:Hypoxia / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000502:physiology; D012621:Seasons; D013482:Superoxide Dismutase / Q000096:biosynthesis; D025801:Ubiquitin / Q000096:biosynthesis; D015854:Up-Regulation
D000199:Actins; D000447:Aldehydes; D000975:Antioxidants; D006360:Heat-Shock Proteins; D025801:Ubiquitin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D045647:DNA Glycosylases; C027576:4-hydroxy-2-nonenal; D006862:Hydrogen Sulfide