title: Effective chemopreventive activity of genistein against human breast cancer cells.
abstract: Chemopreventive and cytotoxic effect of genistein against human breast cancer cell lines was investigated. Genistein inhibited cell proliferation in estrogen receptor-positive (MCF-7) and estrogen receptor-negative (MDA-MB-231) human breast carcinoma cell lines. Cytochrome P450 (CYP) 1A1-mediated ethoxyresorufin O-deethylase (EROD)activity was inhibited by genistein in a concentration-dependent manner. Genistein significantly inhibited 12-Otetradecanoylphorbol-13-acetate (TPA)-induced cyclooxygenase-2 activity and protein expression at the concentrations of 10 (p < 0.05), 25 (p < 0.05) and 50 mM (p < 0.01). In addition, ornithine decarboxylase (ODC) activity was reduced to 53.8 % of the control after 6 h treatment with 50 mM genistein in MCF-7 breast cancer cells. These results suggest that genistein could be of therapeutic value in preventing human breast cancer.
authors.affiliation: Department of Pharmacology, College of Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea.
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Shon
authors.forename: Sun-Dong
authors.initials: SD
authors.lastname: Park
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Nam
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D019363:Cytochrome P-450 CYP1A1 / Q000037:antagonists & inhibitors*; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D008297:Male; D065108:Ornithine Decarboxylase Inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen
chemical_list: D016588:Anticarcinogenic Agents; D016861:Cyclooxygenase Inhibitors; D065108:Ornithine Decarboxylase Inhibitors; D011960:Receptors, Estrogen; D019833:Genistein; D019363:Cytochrome P-450 CYP1A1; D051546:Cyclooxygenase 2