Proteomic analysis of the autoantibody response following immunization with a single autoantigen.
In most autoimmune diseases, the autoantibody response is directed against several antigens of the target organ whose identification is crucial for understanding the physiopathological process. Thus, technologies allowing a characterization of the whole autoantibody pattern of both human and experimental autoimmune diseases are required. Here we have used immunoproteomic analysis of human epidermal extracts to characterize the diversity of the anti-desmosome antibody response induced in normal mice immunized with desmoglein 1, the major autoantigen of pemphigus foliaceus, an autoimmune blistering skin disease. In particular, this analysis enables us to characterize the binding properties of anti-desmosome mAbs derived from these mice and to show that the autoantibody response induced upon immunization with a single autoantigen targets different epidermal autoantigens with a pattern similar to that observed in certain variety of human pemphigus.
Mouquet
Hugo
H
Inserm, U519 and IFR23, Faculté de Médecine et de Pharmacie, Rouen, France.
Drouot
Laurent
L
Charlionnet
Roland
R
Arnoult
Christophe
C
Bonnet-Bayeux
Florence
F
Thomas
Marlène
M
Leprince
Jérôme
J
Joly
Pascal
P
Tron
François
F
Gilbert
Danièle
D
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D051183:Desmoglein 1 / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D051179:Plakins / Q000276:immunology; D011498:Protein Precursors / Q000737:chemistry / Q000276:immunology; D040901:Proteomics; D012867:Skin / Q000276:immunology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001324:Autoantigens; D051183:Desmoglein 1; D008565:Membrane Proteins; C491674:PPL protein, human; D051179:Plakins; D011498:Protein Precursors; C100754:envoplakin