Influence of the pH of the cultivation medium on survival and development of stage III larvae of Anisakis simplex.
The cultivation was done on Eagle's media as well as Eagle's media enriched with the supplement of 10 and 20% of bovine serum. In the media without serum the larvae lived significantly shorter and did not get through molting. They lived the longest and came through molting the fastest in the media with pH 2.0 supplemented with 20% SB. In the media with the pH 4.0 and 7.3 the larvae lived significantly shorter and only a small proportion reached stage IV.
Dziekońska-Rynko
J
J
Department of Parasitology, University of Warmia and Mazury, 14 Zołnierska Street, 10-561 Olsztyn.
Rokicki
J
J
Jabłonowski
Z
Z
Białowas
K
K
D000818:Animals; D017163:Anisakis / Q000254:growth & development*; D002417:Cattle; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D005399:Fishes / Q000469:parasitology; D048788:Growth and Development; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000254:growth & development*; D018978:Molting; D009068:Movement / Q000502:physiology; D010274:Parasitology / Q000379:methods*; D015996:Survival Rate
D003470:Culture Media