title: Infrared multiphoton dissociation spectroscopic analysis of peptides and oligosaccharides by using fourier transform ion cyclotron resonance mass spectrometry with a midinfrared free-electron laser.
abstract: The fragmentation of peptides and oligosaccharides in the gas phase was investigated by means of electrospray ionization Fourier transform ion cyclotron resonance (FTICR) mass spectrometry coupled with dissociation by a laser-cleavage infrared multiphoton dissociation (IRMPD) technique. In this technique, an IR free-electron laser is used as a tunable source of IR radiation to cause cleavage of the ionized samples introduced into the FTICR cell. The gas-phase IRMPD spectra of protonated peptides (substance P and angiotensin II) and two sodiated oligosaccharides (sialyl Lewis X and lacto-N-fucopentaose III) were obtained over the IR scan range of 5.7-9.5 microm. In the IRMPD spectra for the peptide, fragment ions are observed as y/b-type fragment ions in the range 5.7-7.5 microm, corresponding to cleavage of the backbone of the parent amino acid sequence, whereas the spectra of the oligosaccharides have major peaks in the range 8.4-9.5 microm, corresponding to photoproducts of the B/Y type.
authors.affiliation: Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology, 2-41-6 Aomi, Koto-ku, Tokyo 135-0064, Japan. k-fukui@aist.go.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Fukui
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Takada
authors.forename: Tetsumi
authors.initials: T
authors.lastname: Sumiyoshi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Imai
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D004583:Electrons; D005583:Fourier Analysis; D007834:Lasers; D009844:Oligosaccharides / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D013055:Spectrophotometry, Infrared / Q000295:instrumentation / Q000379:methods
chemical_list: D009844:Oligosaccharides; D010455:Peptides