Proteome alterations in human host cells infected with coxsackievirus B3.
Coxsackievirus B3 (CVB3) is a common factor in human myocarditis. The interplay between host factors and virus components is crucial for the fate of the infected cells. Despite that, host protein responses, which characterize CVB3-induced diseases, have not yet been determined in detail. To investigate the nature of modified protein patterns in infected human cells compared with uninfected cells, two-dimensional gel electrophoresis in combination with matrix-assisted laser desorption/ionization-mass spectrometry were used. The regulated proteins, e.g. nucleophosmin (nucleolar protein B23), lamin, the RNA-binding protein UNR and the p38 mitogen-activated protein kinase, were sorted according to their functional groups and interpreted in the context of the myocarditis process.
Rassmann
Alexander
A
Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Beutenbergstrasse 11a, D-07745 Jena, Germany.
Henke
Andreas
A
Institute of Virology and Antiviral Therapy, Medical Center, Friedrich Schiller University, Hans-Knoell-Strasse 2, D-07745 Jena, Germany.
Zobawa
Monica
M
Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Carlsohn
Marc
M
Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Beutenbergstrasse 11a, D-07745 Jena, Germany.
Saluz
Hans-Peter
HP
Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Beutenbergstrasse 11a, D-07745 Jena, Germany.
Grabley
Susanne
S
Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute Beutenbergstrasse 11a, D-07745 Jena, Germany.
Lottspeich
Friedrich
F
Institute of Virology and Antiviral Therapy, Medical Center, Friedrich Schiller University, Hans-Knoell-Strasse 2, D-07745 Jena, Germany.
Munder
Thomas
T
Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Beutenbergstrasse 11a, D-07745 Jena, Germany.
D002460:Cell Line; D003384:Coxsackievirus Infections / Q000209:etiology / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D029822:Enterovirus B, Human / Q000145:classification / Q000472:pathogenicity*; D006367:HeLa Cells; D006801:Humans; D034882:Lamins / Q000302:isolation & purification / Q000378:metabolism; D009205:Myocarditis / Q000209:etiology / Q000378:metabolism; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism; D000090243:Nucleophosmin; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D048051:p38 Mitogen-Activated Protein Kinases / Q000302:isolation & purification / Q000378:metabolism
C079950:CSDE1 protein, human; D004268:DNA-Binding Proteins; D034882:Lamins; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D020543:Proteome; D016601:RNA-Binding Proteins; D000090243:Nucleophosmin; D048051:p38 Mitogen-Activated Protein Kinases