In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains.
BACKGROUND
Helicobacter pylori has now been widely recognized as a causative agent of gastroduodenal diseases. The development of safer anti- H. pylori compounds is desirable due to the antibiotic-resistant strains emerged to date.


METHODS
We successfully developed the compounds of Rice-fluid derived from unpolished, polished, and usually cooked Japanese rice, and investigated their in vitro antibacterial activities by means of the Time-Kill-Curve methods against various species of bacteria including H. pylori strains.


RESULTS
All of the compounds revealed keen bactericidal activities against H. pylori, followed by Streptococcus pneumoniae and Campylobacter jejuni strains, but failed to affect the viability of other bacterial species investigated including staphylococci, enterococci, Pseudomonas aeruginosa, and other gram-negative rods belonging to the family Enterobacteraceae. The bactericidal activities were demonstrated to be time- and concentration-dependent.


CONCLUSIONS
The compounds of Rice-fluid are considered to be potentially new and safe therapeutic regimens against H. pylori infections. The mechanism of their bactericidal activities against H. pylori strains remains to be elucidated.
Kawakami
Yoshiyuki
Y
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, Matsumoto, 390-8621, Japan. yk23724@gipac.shinshu-u.ac.jp
Oana
Kozue
K
Hayama
Masayoshi
M
Ota
Hiroyoshi
H
Takeuchi
Masahiko
M
Miyashita
Kazuhiro
K
Matsuzawa
Tsunetomo
T
Kanaya
Kiyomi
K