title: Measurement of bisphenol A concentrations in human colostrum.
abstract: Bisphenol A (BPA), an estrogenic endocrine disrupting chemical, has been reported to affect embryos and alter their postnatal development. In the present study, we measured the concentrations of BPA in human colostrum by a competitive enzyme-linked immunosorbent assay (ELISA) with the aim of understanding the present status of BPA burden in human breast milk in Shizuoka, Japan. Human colostral samples were collected from 101 healthy mothers within three days after delivery. The BPA concentrations of colostral samples were estimated by ELISA after the acetonitrile extraction and solid phase extraction column purification. BPA in 101 samples was detected in the concentration range of 1-7 ng ml(-1). The mean concentration of BPA was 3.41+/-0.13 (mean+/-SD) ng ml(-1). This is the first demonstration as to what BPA concentrations are in human colostrum. The BPA concentrations in colostrum were higher than those in blood sera samples obtained from healthy women in a previous study. In our study, there was no significant correlation between the concentrations of BPA in colostrum and the age and parity of mothers.
authors.affiliation: School of Food and Nutritional Sciences, Laboratory of Microbiology and Host Defenses, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. kuruto@u-shizuoka-ken.ac.jp
authors.forename: Ryoko
authors.initials: R
authors.lastname: Kuruto-Niwa
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Tateoka
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Usuki
authors.forename: Ryushi
authors.initials: R
authors.lastname: Nozawa
mesh_terms: D001559:Benzhydryl Compounds; D003126:Colostrum / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008423:Maternal Age; D010298:Parity; D010636:Phenols / Q000032:analysis*; D011247:Pregnancy; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds