title: [Purification and properties of isoflavone-glucosidase].
abstract: A high activity isoflavone-glucosidase, which hydrolysis glycosides, was obtainde using liquid fermentation from Absidia sp. R strain. The isoflavone-glucosidase was purified 11 folds with yielding rate of 10.9% after ammonium sulfate precipitation and DEAE-Cellocuse (DE-52) ion exchange chromatography. SDS-PAGE results showed that the molecular weight is 53kD. And the optimum temperature, the optimum pH, Km and pI of the enzyme are 50 deegrees C, 5.0, 1.3 x 10(-2) mol/L and 3.2, respectively. The isoflavone-glucosidase is also rather stable under 60 degrees C and in pH range from 5.0 to 7.0. The enzyme can be activated by Co2+ and Ca2+, and be inhibited by Ag+ and Cu2+.
authors.affiliation: College of Life Science, Liaoning Normal University, Dalian 116029, China. xmj1222@sina.com
authors.forename: Ming-Jie
authors.initials: MJ
authors.lastname: Xie
authors.forename: Ming
authors.initials: M
authors.lastname: Song
authors.forename: Cui-Xia
authors.initials: CX
authors.lastname: Zou
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Xu
authors.forename: Ming-Chun
authors.initials: MC
authors.lastname: Lu
authors.forename: Feng-Xie
authors.initials: FX
authors.lastname: Jin
mesh_terms: D020095:Absidia / Q000201:enzymology*; D005959:Glucosidases / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000378:metabolism*; D013696:Temperature
chemical_list: D007529:Isoflavones; D005959:Glucosidases