Glutamate receptor-mediated inhibition of L-glutamate efflux from cerebral cortex in vitro.
We tested whether glutamate receptor ligands affect oxygen-glucose deprivation-evoked L-glutamate efflux from adult rat cerebrocortical prisms. The uncompetitive NMDA antagonist AR-R15896AR inhibited efflux (IC50 34 microM, 87% maximal inhibition). AMPA/kainate receptor blockade (NBQX, 100 microM) or Group II metabotropic glutamate receptor activation (DCG-IV, 10 microM) inhibited efflux (41%, 67% respectively) but Group I mGluR blockade (CPCCOEt/MPEP, 10 microM) was without effect. These data support a modulatory effect of glutamate receptors on L-glutamate efflux.
Hainsworth
Atticus H
AH
Pharmacology Research Group, Leicester School of Pharmacy, De Montfort University, Leicester, LE1 9BH, UK. ahainsworth@dmu.ac.uk
Nelson
Rachael M
RM
Lambert
David G
DG
Green
A Richard
AR
Webb
Tania E
TE
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000172:deficiency; D018698:Glutamic Acid / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism; D066298:In Vitro Techniques; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology*; D011725:Pyridines / Q000494:pharmacology; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000502:physiology*; D013997:Time Factors
D018691:Excitatory Amino Acid Antagonists; D011725:Pyridines; D011810:Quinoxalines; D017470:Receptors, Glutamate; C096406:alpha-phenyl-2-pyridineethanamine dihydrochloride; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; D018698:Glutamic Acid; D005947:Glucose