Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography.
A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4:1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their (1)H NMR and (13)C NMR spectra.
Han
Quan Bin
QB
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, ShaTin N.T., Hong Kong.
Song
Jing Zheng
JZ
Qiao
Chun Feng
CF
Wong
Lina
L
Xu
Hong Xi
HX
D003377:Countercurrent Distribution / Q000379:methods*; D013237:Stereoisomerism; D044004:Xanthones / Q000302:isolation & purification*
D044004:Xanthones; C052659:gambogic acid