title: [Detection of Toxoplasma gondii DNA by PCR in mother's blood, amniotic fluid and child's blood in selected cases of pathological pregnancy].
abstract: Prenatal screening of Toxoplasma gondii infection is controversial. The diagnosis is based on serological tests detecting IgM and IgG antibodies against T. gondii, but interpretation of these tests results is often confusing. It is commonly made retrospectively when serological screening indicates a possibility of recent infection. Most women have antibodies against T. gondii and serial testing is required only in monitoring of pregnancies where initial screening is negative. The introduction of Polymerase Chain Reaction (PCR) assay for detection of Toxoplasma gondii DNA in selected cases of pathologic pregnancies has permitted a more accurate and faster diagnosis of congenital toxoplasmosis infections.
authors.affiliation: Katedra i Klinika Patologii Ciazy i Rozrodczości w Zabrzu, Slaskiej Akademii Medycznej 41 - 800 Zabrze, ul. Janika 18.
authors.forename: H
authors.initials: H
authors.lastname: Sławska
authors.forename: J
authors.initials: J
authors.lastname: Pendzich
authors.forename: B
authors.initials: B
authors.lastname: Czuba
authors.forename: U
authors.initials: U
authors.lastname: Mazurek
authors.forename: J
authors.initials: J
authors.lastname: Gola
authors.forename: T
authors.initials: T
authors.lastname: Wilczok
authors.forename: K
authors.initials: K
authors.lastname: Kamiński
mesh_terms: D000328:Adult; D000649:Amniocentesis / Q000379:methods; D000653:Amniotic Fluid / Q000469:parasitology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D016054:DNA, Protozoan / Q000097:blood; D005260:Female; D006801:Humans; D007231:Infant, Newborn / Q000097:blood*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D011296:Prenatal Diagnosis / Q000379:methods; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000097:blood* / Q000175:diagnosis; D014125:Toxoplasmosis, Congenital / Q000097:blood* / Q000175:diagnosis / Q000235:genetics
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan