title: Exotic flora dependence of an unusual Brazilian propolis: the pinocembrin biomarker by capillary techniques.
abstract: Significant amounts of pinocembrin (>10%), a dihydroxy-flavanone, was found in the composition of an unusual brand of a subtropical Brazilian propolis. Incidentally, this sealing material was obtained from hives surrounding a large forestry site based on a single exotic flora, namely poplar (Populus sp.). Examination of the different botanical parts of poplar revealed the buds as the main source of the flavanone. Techniques used for the establishment of the chemical correlation between the propolis brand and the poplar buds were TLC/densitometry, capillary GC-MS in the e.i. mode, and CZE with DAD monitoring. Since color enhancement after Al3+ complexation applies just for more hydroxylated flavonoids, the alternative techniques herein applied were of value for pinocembrin detection and estimation. Analytical data indicated the dominance of the main phenolic pinocembrin biomarker as well as the presence of other related flavonoids in the botanical source and in the propolis derived thereof.
authors.affiliation: Biomass Chemo/Biotechnology Laboratory (LQBB), Department of Pharmacy, Federal University of Parana, R. Lothario Meissner 632, Subsede Botânico, Curitiba PR (80210-170), Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Adelmann
authors.forename: Mauricio
authors.initials: M
authors.lastname: Passos
authors.forename: Daniel Henrique
authors.initials: DH
authors.lastname: Breyer
authors.forename: Maria Helena Rocha
authors.initials: MH
authors.lastname: dos Santos
authors.forename: Cesar
authors.initials: C
authors.lastname: Lenz
authors.forename: Natalício Ferreira
authors.initials: NF
authors.lastname: Leite
authors.forename: Fernando Mauro
authors.initials: FM
authors.lastname: Lanças
authors.forename: José Domingos
authors.initials: JD
authors.lastname: Fontana
mesh_terms: D000535:Aluminum / Q000737:chemistry; D000818:Animals; D001516:Bees; D015415:Biomarkers; D001938:Brazil; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002855:Chromatography, Thin Layer; D003720:Densitometry; D019075:Electrophoresis, Capillary; D044950:Flavanones / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D032107:Populus / Q000737:chemistry*; D011429:Propolis / Q000737:chemistry*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
chemical_list: D015415:Biomarkers; D044950:Flavanones; D005419:Flavonoids; D007202:Indicators and Reagents; C016063:pinocembrin; D011429:Propolis; D000535:Aluminum