In vitro evaluation of biospecific and pseudobiospecific ligands aimed at extracorporeal treatment for immunoglobulin E removal.
This work investigated the potential use of an alternative adsorbent to anti-immunoglobulin E (IgE)-agarose for IgE selective adsorption therapy. A screening of several commercially available adsorbents (Concanavalin A, Lens culinaris[Lc], d-tryptophan, poly-l-lysine, and aminohexyl immobilized on agarose) was done through batch system assays, considering some criteria, such as adsorption capacity, selectivity, and biocompatibility. In the Lc-agarose adsorbent, total IgE, and specific IgE--for the airborne allergens Dermatophagoides pteronyssinus and Blomia tropicalis--were significantly better removed (63, 58, and 59%, respectively) than immunoglobulin G (19%), immunoglobulin A (33%), immunoglobulin M (9%), and albumin (18%). This adsorbent was packed into a column and the effect of superficial velocity, ratio of plasma volume to bed volume, number of perfusions, and temperature on IgE adsorption were evaluated. In vitro simulation of therapeutic adsorption (single perfusion) indicated that about 50% of total IgE could be eliminated.
Duarte
Isa S
IS
School of Chemical Engineering, State University of Campinas, Campinas, São Paulo, Brazil.
Zollner
Ricardo L
RL
Bueno
Sonia M A
SM
D000327:Adsorption; D001781:Blood Component Removal / Q000379:methods*; D002847:Chromatography, Agarose / Q000379:methods; D050577:Complement C3-C5 Convertases / Q000378:metabolism; D005112:Extracorporeal Circulation / Q000379:methods; D006801:Humans; D006967:Hypersensitivity / Q000628:therapy; D007073:Immunoglobulin E / Q000378:metabolism*; D007163:Immunosorbent Techniques; D007164:Immunosorbents / Q000493:pharmacokinetics* / Q000627:therapeutic use; D008024:Ligands; D037121:Plant Lectins / Q000493:pharmacokinetics / Q000627:therapeutic use; D011107:Polylysine / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000627:therapeutic use; D012685:Sepharose / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000627:therapeutic use; D014364:Tryptophan / Q000493:pharmacokinetics / Q000627:therapeutic use
D007164:Immunosorbents; D008024:Ligands; D037121:Plant Lectins; C028326:concanavalin A-sepharose; C033619:lentil lectin; C041885:polylysine-agarose; D011107:Polylysine; D007073:Immunoglobulin E; C024903:aminohexyl-sepharose; D014364:Tryptophan; D012685:Sepharose; D050577:Complement C3-C5 Convertases