title: Neutrophil-mediated liver injury during hepatic ischemia-reperfusion in rats.
abstract: BACKGROUND
  Neutrophil plays an important role in hepatic ischemia-reperfusion injury. We investigated neutrophil infiltration in liver tissue, Kupffer cells' role in neutrophil accumulation, and apoptosis and regeneration of hepatocytes in liver ischemia-reperfusion injury.
  
  
  METHODS
  Vascular microclamps were placed across the pedicles of the median and left lateral lobes for 90 minutes after 30% hepatectomy with the resection of caudate, right lateral and quadrate lobes and papillary process. Gadolinium chloride (GdCl(3)) was used to destroy Kupffer cells. Neutrophil activity was inhibited with Urge-8, a monoclonal antibody against neutrophil produced in our laboratory. GdCl(3) (10 mg/kg) and Urge-8 (50 mg/kg) were given intravenously in respective groups. Ischemia control, GdCl(3) and Urge-8 groups were compared.
  
  
  RESULTS
  Following hepatic reperfusion, serum interleukin-8 (IL-8) levels and hepatic neutrophil counts peaked at 3 hours, and peak concentrations of alanine aminotransferase (ALT) occurred at 6 hours. Animals of the control group showed increases in neutrophil infiltration in liver tissue, liver enzyme levels, and apoptosis index of hepatocytes and decreases in overall survival rate and proliferating cell nuclear antigen (PCNA) expression of hepatocytes. The survival rates and PCNA proportion of hepatocytes were higher and the levels of hepatic neutrophil infiltration, liver enzymes, and hepatocyte apoptosis after reperfusion were lower in the GdCl(3) and Urge-8 groups than those in the ischemia control group.
  
  
  CONCLUSIONS
  Blockades of Kupffer cells' activity and neutrophil infiltration by GdCl(3) and Urge-8 eliminate neutrophil-mediated hepatic injury and enhance subsequent hepatic regeneration during liver ischemia-reperfusion.
authors.affiliation: Department of General Surgery, Qilu Hospital, Shandong University, Jinan 250012, China. yxchen@sdu.edu.cn
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Chen
authors.forename: Motomichi
authors.initials: M
authors.lastname: Sato
authors.forename: Kanji
authors.initials: K
authors.lastname: Kawachi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Abe
mesh_terms: D000818:Animals; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007657:Ketone Bodies / Q000097:blood; D008099:Liver / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism / Q000503:physiopathology; D008111:Liver Function Tests; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D007657:Ketone Bodies