Measurement of bisphenol A concentrations in human colostrum.
Bisphenol A (BPA), an estrogenic endocrine disrupting chemical, has been reported to affect embryos and alter their postnatal development. In the present study, we measured the concentrations of BPA in human colostrum by a competitive enzyme-linked immunosorbent assay (ELISA) with the aim of understanding the present status of BPA burden in human breast milk in Shizuoka, Japan. Human colostral samples were collected from 101 healthy mothers within three days after delivery. The BPA concentrations of colostral samples were estimated by ELISA after the acetonitrile extraction and solid phase extraction column purification. BPA in 101 samples was detected in the concentration range of 1-7 ng ml(-1). The mean concentration of BPA was 3.41+/-0.13 (mean+/-SD) ng ml(-1). This is the first demonstration as to what BPA concentrations are in human colostrum. The BPA concentrations in colostrum were higher than those in blood sera samples obtained from healthy women in a previous study. In our study, there was no significant correlation between the concentrations of BPA in colostrum and the age and parity of mothers.
Kuruto-Niwa
Ryoko
R
School of Food and Nutritional Sciences, Laboratory of Microbiology and Host Defenses, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. kuruto@u-shizuoka-ken.ac.jp
Tateoka
Yumiko
Y
Usuki
Yasuteru
Y
Nozawa
Ryushi
R
D001559:Benzhydryl Compounds; D003126:Colostrum / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008423:Maternal Age; D010298:Parity; D010636:Phenols / Q000032:analysis*; D011247:Pregnancy; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds