title: Normal human fibroblasts exposed to high- or low-dose ionizing radiation: differential effects on mitochondrial protein import and membrane potential.
abstract: How oxidative metabolism modulates effects of ionizing radiation is incompletely understood. Because mitochondria participate in oxidative metabolism, we investigated the modulation of mitochondrial protein import and membrane potential (DeltaPsi) in irradiated cells. Our data show that effects at low dose cannot be predicted from effects at high dose. When density-inhibited normal human fibroblasts were exposed to a toxic dose of 4 Gy, protein import into mitochondria isolated from these cells was decreased. In contrast, protein import into mitochondria isolated from low-dose-irradiated (10 cGy) cells was enhanced, suggesting that mitochondria may play a crucial role in low-dose-induced adaptive responses. At high dose, import defects were not solely due to changes in mitochondrial DeltaPsi, and modulation of import was not tightly linked to the cellular capacity to repair radiation damage. Another striking observation is that in proliferating nonirradiated cells, mitochondrial protein import and DeltaPsi were regulated in a cell cycle-dependent manner, being lower in S phase than in G (1). Interestingly, when quiescent G (0)/G (1) phase cells exposed to high-dose radiation were stimulated to proliferate, events associated with S phase, but not G (1), significantly affected import. The strategy described here may serve as novel end points to study radiation-induced effects.
authors.affiliation: Department of Radiology, UMDNJ-New Jersey Medical School, Newark, 07101, USA. Painde@umdnj.edu
authors.forename: Badri N
authors.initials: BN
authors.lastname: Pandey
authors.forename: Donna M
authors.initials: DM
authors.lastname: Gordon
authors.forename: Sonia M
authors.initials: SM
authors.lastname: De Toledo
authors.forename: Debkumar
authors.initials: D
authors.lastname: Pain
authors.forename: Edouard I
authors.initials: EI
authors.lastname: Azzam
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D006801:Humans; D008564:Membrane Potentials / Q000528:radiation effects; D008565:Membrane Proteins / Q000378:metabolism* / Q000528:radiation effects; D008928:Mitochondria / Q000378:metabolism* / Q000528:radiation effects; D011829:Radiation Dosage; D011839:Radiation, Ionizing
chemical_list: D008565:Membrane Proteins