DNA fingerprinting elicited evolutionary trend of oral Candida tropicalis isolates from diverse geographic locales.
OBJECTIVE
To study molecular profiles of oral Candida tropicalis isolates from five different geographic locales to determine the molecular diversity, clonality and evolutionary trends of this opportunistic pathogen.


METHODS
A total of 36 strains from five countries (China, Canada, Scotland, Japan and Tanzania) were genotyped by PCR fingerprinting with 11 separate primers. Of these, primers RSG9, RSG8, T3B and RSD12 generated complex fingerprinting patterns.


RESULTS
Three significantly dissimilar profiles were derived from the primer T3B and particularly focused on tDNA suggested the prevalence of genetic subtypes within the species. Comparison of tDNA and rDNA (RSD12) fingerprints of C. tropicalis suggested that rDNA is much more heterogeneous than the relatively distinct tDNA. Further analysis of similarity coefficient (SAB) of gel profiles derived from computer-generated dendrograms indicated some degree of similarity in isolates from five-disparate geographic locales as well as the presence of unique isotypes in each region.


CONCLUSIONS
This study demonstrates the evolutionary divergence of distinct genetic subgroups within Candida tropicalis .
Dassanayake
R S
RS
Faculty of Dentistry, University of Hong Kong, Hong Kong SAR, China.
Samaranayake
Y H
YH
Yau
Jjy
J
Samaranayake
L P
LP
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000382:microbiology; D041022:Candida tropicalis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D006801:Humans; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique
D004271:DNA, Fungal; D012335:RNA, Ribosomal; D012343:RNA, Transfer