Flow-injection chemiluminescence determination of ofloxacin and levofloxacin in pharmaceutical preparations and biological fluids.
A novel, rapid and sensitive analytical method is described for determination of ofloxacin and levofloxacin by enhanced chemiluminescence (CL) with flow-injection sampling. The method is based on the CL reaction of the Ce(IV)-Na2S2O4-ofloxacin/levofloxacin-H2SO2 system. The enhanced CL mechanism was developed and the optimum conditions for CL emission were investigated. The CL intensity was correlated linearly (r = 0.9988) with the concentration of ofloxacin (or levofloxacin) in the range of 1.0 x 10(-8) - 1.0 x 10(-7) g ml(-1) and 1.0 x 10(-7) - 6.0 x 10(-6) g ml(-1). The detection limit (S/N = 3) is 7 x 10(-9) g ml(-1). The relative standard derivation (RSD, n = 11) is 2.0% for ofloxacin at 4 x 10(-7) g ml(-1) and for levofloxacin at 6 x 10(-7) g ml(-1). This method has been successfully applied for the determination of ofloxacin and levofloxacin in pharmaceutical preparations and biological fluids with satisfactory results.
Sun
Hanwen
H
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Boading, China. hanwen@mail.hbu.cn
Li
Liqing
L
Chen
Xueyan
X
D000892:Anti-Infective Agents, Urinary / Q000032:analysis*; D002563:Cerium / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000295:instrumentation / Q000379:methods*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D064704:Levofloxacin; D049449:Luminescence; D008956:Models, Chemical; D015242:Ofloxacin / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000379:methods
D000892:Anti-Infective Agents, Urinary; D004364:Pharmaceutical Preparations; D002563:Cerium; D064704:Levofloxacin; D015242:Ofloxacin