title: Burkholderia silvatlantica sp. nov., a diazotrophic bacterium associated with sugar cane and maize.
abstract: In a previous study, nitrogen-fixing isolates were recovered from the rhizosphere of maize and from surface-sterilized leaves of sugar cane cultivated in Rio de Janeiro, Brazil. On the basis of 16S rRNA gene sequence similarities, these isolates were identified as belonging to the genus Burkholderia, and whole-cell-protein profiles demonstrated that they are closely related to each other. In the present study, novel isolates were recovered from the roots of different sugar-cane varieties cultivated in diverse geographical regions of Brazil. Twenty-one nitrogen-fixing isolates were analysed using polyphasic taxonomy criteria, including DNA-DNA relatedness, 16S rRNA gene sequence similarities, fatty acid profiles, whole-cell-protein patterns and multilocus enzyme electrophoresis profiles, as well as morphological, physiological and biochemical characterization. The analysis confirmed that these isolates belong to a novel species within the genus Burkholderia, for which the name Burkholderia silvatlantica sp. nov. is proposed. The type strain, SRMrh-20(T) (=LMG 23149(T)=ATCC BAA-1244(T)), was isolated from the rhizosphere of maize var. Avantis A2345 cultivated in Seropédica, Rio de Janeiro.
authors.affiliation: Embrapa Agrobiology, km 47, BR 465, C.P. 74505, Seropédica, Rio de Janeiro, Brazil.|Universidade Federal Rural do Rio de Janeiro, km 45, BR 465, C.P. 74505, Seropédica, Rio de Janeiro, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Perin
authors.affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, Mexico.
authors.forename: L
authors.initials: L
authors.lastname: Martínez-Aguilar
authors.affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: Paredes-Valdez
authors.affiliation: Embrapa Agrobiology, km 47, BR 465, C.P. 74505, Seropédica, Rio de Janeiro, Brazil.
authors.forename: J I
authors.initials: JI
authors.lastname: Baldani
authors.affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, Mexico.
authors.forename: P
authors.initials: P
authors.lastname: Estrada-de Los Santos
authors.affiliation: Embrapa Agrobiology, km 47, BR 465, C.P. 74505, Seropédica, Rio de Janeiro, Brazil.
authors.forename: V M
authors.initials: VM
authors.lastname: Reis
authors.affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Caballero-Mellado
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D001938:Brazil; D019117:Burkholderia / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D031786:Saccharum / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D003313:Zea mays / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S