Geotrichum candidum link 1809: hydrolases activity and own method of digestive tract strains biotyping.
Hydrolase activity of (enzymograms, biotypes) in Geotrichum candidum, one of the poorly described pathogenic fungi, was studied 81 strains were isolated from oral cavity and faeces of patients with gastrointestinal tract disorders. Axenic strains were differentiated with API 20C Aux and API ZYM tests. Then, enzymograms and biotypes were determined for all strains based on the activity of 19 hydrolases. High variability of enzymograms (17 different types) was found. The highest activity was noted in case of: e2 - alkaline phosphatase, e6 - leucine arylamidase, e11 - acid phosphatase. E5 - lipase, e7 - valine arylamidase, e12 - naphtol-AS-BI-phosphohydrolase and e17 - beta-glucosidase were used for biotyping procedures. Our own system of biotyping of 81 strains of G. candidum was based on the mathematical binominal distribution formula (1 : 4 : 6 : 4 : 1) - all "+"; one "-", three "+"; two "two "+"; three "-", one "+"; all "-". We have found: A (11.1 +/- 3.5%), BI (6.17 +/- 2.67%), B2 (1.23 +/- 1.22%), B4 (4.94 +/- 2.41%), C, (1.23 +/-1.22%), C3 (63.0 +/- 5.4%), D2 (9.88 +/-3.31%), D3 (2.47 +/- 1.72%). Among all strains from 8 various biotypes of G. candidum.
Kurnatowska
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A
Chair of Biology and Medical Parasitology, Medical University Al. Kościuszki 85, 90-436 Lódź, Poland.
Białasiewicz
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D
D000135:Acid Phosphatase / Q000378:metabolism; D000465:Algorithms; D000469:Alkaline Phosphatase / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology*; D005847:Geotrichosis / Q000469:parasitology*; D005848:Geotrichum / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism; D009055:Mouth / Q000382:microbiology; D016533:Mycological Typing Techniques; D001617:beta-Glucosidase / Q000378:metabolism
D006867:Hydrolases; D008049:Lipase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D001617:beta-Glucosidase