title: Distinct roles of the Pumilio and FBF translational repressors during C. elegans vulval development.
abstract: The C. elegans PUF and FBF proteins regulate various aspects of germline development by selectively binding to the 3' untranslated region of their target mRNAs and repressing translation. Here, we show that puf-8, fbf-1 and fbf-2 also act in the soma where they negatively regulate vulvaI development. Loss-of-function mutations in puf-8 cause ectopic vulval differentiation when combined with mutations in negative regulators of the EGFR/RAS/MAPK pathway and suppress the vulvaless phenotype caused by mutations that reduce EGFR/RAS/MAPK signalling. PUF-8 acts cell-autonomously in the vulval cells to limit their temporal competence to respond to the extrinsic patterning signals. fbf-1 and fbf-2, however, redundantly inhibit primary vulval cell fate specification in two distinct pathways acting in the soma and in the germline. The FBFs thereby ensure that the inductive signal selects only one vulval precursor cell for the primary cell fate. Thus, translational repressors regulate various aspects of vulval cell fate specification, and they may play a conserved role in modulating signal transduction during animal development.
authors.affiliation: Zoologisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.
authors.forename: Claudia B
authors.initials: CB
authors.lastname: Walser
authors.forename: Gopal
authors.initials: G
authors.lastname: Battu
authors.forename: Erika Fröhli
authors.initials: EF
authors.lastname: Hoier
authors.forename: Alex
authors.initials: A
authors.lastname: Hajnal
mesh_terms: D000818:Animals; D019521:Body Patterning; D017173:Caenorhabditis elegans / Q000254:growth & development* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D003001:Cloning, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000378:metabolism*; D015398:Signal Transduction; D014844:Vulva / Q000196:embryology*
chemical_list: D029742:Caenorhabditis elegans Proteins; C510538:PUF-8 protein, C elegans; D016601:RNA-Binding Proteins; C109286:fem-3-binding protein, C elegans