title: Serological investigation in children infected with Ascaris lumbricoides.
abstract: The study included 260 hospitalised children with suspected infection with human ascaris. In serological diagnostics a protein antigen obtained from Ascaris lumbricoides was used. ELISA method was applied. IgG antibodies were detected. Positive results were found in 15% of the examined children. No relation to the gender or demographic conditions was found. The most frequently observed symptoms in the patients with Ascaris lumbricoides were: abdominal pain--87%, diarrhoea 15%. In 31% of the cases eosinophilia was found. Scatoscopy was carried out for all the patients, using the PARASEPT system and Kato and Miura methods as well as decantation and flotation. The examination, which was repeated three times, did not show cysts or eggs. Serological investigation exhibits higher sensitivity than the traditional methods. Their use in recognising ascariasis in humans significantly facilitates diagnostic procedures, especially in the lung phase of the disease, the larval stage or in cases of infection with an individual parasite, when the faeces samples do not contain the eggs. Serological investigation is also useful in all cases of suspected VLM.
authors.affiliation: Department of Clinical Microbiology, Polish Mother Memorial Hospital and Research Institute, 93- 338 Lódź, 281/289 Rzgowska Street.
authors.forename: E
authors.initials: E
authors.lastname: Małafiej
authors.forename: E
authors.initials: E
authors.lastname: Spiewak
mesh_terms: D000293:Adolescent; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis / Q000145:classification; D001196:Ascariasis / Q000175:diagnosis*; D017164:Ascaris lumbricoides / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000145:classification; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis*; D008297:Male; D010270:Parasite Egg Count; D011044:Poland; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G