title: Blood leukocyte responses in rats vaccinated with cDNA encoding glutathioinie-S-transferase of Fasciola hepatica.
abstract: Changes in blood leucocyte levels were investigated in Spraque-Dowley rats vaccinated with cDNA or protein of glutathione S-transferase (GST) of F. hepatica and subsequently challenged with metacercariae of the liver fluke. The analysis of the leucocyte responses measured in vaccinated rats suggests that the form of antigen used for vaccination influenced dynamics of white blood cell response to the fluke infection. The most clear differences were observed in neutrophil and eosinophil levels. The weakest reaction of these cells to the challenge infection was observed in rats vaccinated twice with cDNA. In contrast, in rats which received the first antigen dose as cDNA and the second vaccination with GST protein, both neutrophil and eosinophil responses were much higher, especially at 5 and 9 WAI.
authors.affiliation: W. Stefanski Institute of Parasitology PAS, 00-818 Warszawa, Twarda 51/55.
authors.forename: I
authors.initials: I
authors.lastname: Wedrychowicz
authors.forename: L
authors.initials: L
authors.lastname: Jedlina-Panasiuk
authors.forename: P
authors.initials: P
authors.lastname: Szymanski
authors.forename: K
authors.initials: K
authors.lastname: Bieńkowska-Szewczyk
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D018076:DNA, Complementary / Q000008:administration & dosage; D018509:DNA, Helminth / Q000008:administration & dosage*; D004804:Eosinophils / Q000276:immunology*; D005210:Fasciola hepatica / Q000201:enzymology*; D005211:Fascioliasis / Q000276:immunology / Q000517:prevention & control; D005982:Glutathione Transferase / Q000008:administration & dosage*; D007962:Leukocytes / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014611:Vaccination / Q000379:methods; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D014612:Vaccines; D019444:Vaccines, DNA; D005982:Glutathione Transferase