title: [Structure and function of pore-forming proteins from bacteria of the genus Yersinia: I. Isolation and a comparison of physicochemical properties and functional activity of Yersinia porins].
abstract: The molecular organization and functional activity of porins isolated from the outer membrane (OM) of the Yersinia enterocolitica and three phylogenetically close nonpathogenic Yersinia species (Y. intermedia, Y. kristensenii, and Y. frederiksenii) cultured at 6-8 degrees C were comparatively studied for the first time. The proteins were isolated in two molecular forms (trimeric and monomeric), and their spatial structures were characterized by the methods of optical spectroscopy, CD and intrinsic protein fluorescence. The studied porins were shown to belong to the beta-structural proteins (they have 59-96% total beta structures and 0-17% alpha helices). The spatial structures of the proteins were demonstrated to depend on the nature of the detergent used for solubilization. Unlike the enterobacterial pore-forming proteins, the porin trimers are less stable to sodium dodecyl sulfate (SDS). The spatial structures of the porins become more compact after the substitution of octyl beta-D-glucoside for SDS: the content of beta structures increases and the accessibility of Trp residues to solvent decreases. It was established with the use of the technique of bilayer lipid membranes that the functional properties of the porins are similar to those of the OmpF proteins of Gram-negative bacteria. Trimers are functionally active forms of the porins. Special features of the pore-forming activity of the Yersinia porins were revealed to depend on the microorganism species and the value of the membrane potential.
authors.forename: O P
authors.initials: OP
authors.lastname: Vostrikova
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Kim
authors.forename: G N
authors.initials: GN
authors.lastname: Likhatskaia
authors.forename: K V
authors.initials: KV
authors.lastname: Guzev
authors.forename: T I
authors.initials: TI
authors.lastname: Vakorina
authors.forename: V A
authors.initials: VA
authors.lastname: Khomenko
authors.forename: O D
authors.initials: OD
authors.lastname: Novikova
authors.forename: T F
authors.initials: TF
authors.lastname: Solov'eva
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000502:physiology*; D002942:Circular Dichroism; D018272:Porins / Q000737:chemistry* / Q000502:physiology*; D017433:Protein Structure, Secondary; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D015007:Yersinia / Q000378:metabolism / Q000502:physiology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018272:Porins; D012967:Sodium Dodecyl Sulfate