title: Optically specific detection of D- and L-lactic acids by a flow-injection dual biosensor system with on-line microdialysis sampling.
abstract: A flow-injection dual biosensor system with microdialysis sampling is proposed for the simultaneous determination of D-lactic and L-lactic acids. The dialysate from the microdialysis tube is delivered to a sample loop of the six-way autoinjector and then automatically injected into the flow-injection line with a dual enzyme electrode arranged in perpendicular to the flow direction. The dual enzyme electrode is constructed by hybridizing a poly(1,2-diaminobenzene) film into two sensing parts which respond selectively to D-lactic and L-lactic acids, respectively, without any cross-reactivity. The proposed flow-injection analysis method can be successfully applied to the simultaneous determination of D,L-lactic acids in alcoholic beverages.
authors.affiliation: Oji Scientific Instruments, Amagasaki, Hyogo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nanjo
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yano
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Hayashi
authors.forename: Toshio
authors.initials: T
authors.lastname: Yao
mesh_terms: D000434:Alcoholic Beverages; D015374:Biosensing Techniques; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D017022:Flow Injection Analysis / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry*; D017551:Microdialysis; D008956:Models, Chemical; D055095:Optics and Photonics; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: D019344:Lactic Acid