title: Molecular characterization of the two-component histidine kinase gene from Monilinia fructicola.
abstract: Brown rot of stone fruit caused by Monilinia fructicola (G. Wint) Honey is one of the most common fungal diseases in California. In this study, two laboratory-induced iprodione-resistant (LIR) mutants of M. fructicola were characterized by osmotic sensitivity, virulence on prune and sequence of the two-component histidine kinase gene (Mfos-1). The LIR mutants showed more sensitivity to osmotic stress and lower virulence on prune than their wild-type parent. Analysis of deduced amino acid of Mfos-1 showed that this protein exhibited all the characteristic features of the two-component histidine kinase genes, including osmotic sensing domain, six 90-amino-acid repeat motifs (coiled coil region) and kinase core and response regulator domains. Comparison of DNA sequences of the Mfos-1 from LIR mutants and the wild-type sensitive (S) isolate showed that LIR mutants had single point mutations in the coiled coil region of Mfos-1.
authors.affiliation: Institute of Biotechnology, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, China. zhma@zju.edu.cn
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Ma
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Themis
authors.initials: T
authors.lastname: Michailides
mesh_terms: D000595:Amino Acid Sequence; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000633:toxicity; D001203:Ascomycota / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D025141:Drug Resistance, Fungal / Q000235:genetics; D005638:Fruit / Q000382:microbiology; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005659:Fungicides, Industrial / Q000633:toxicity; D000071677:Histidine Kinase; D006827:Hydantoins / Q000633:toxicity; D008969:Molecular Sequence Data; D009995:Osmosis; D010935:Plant Diseases / Q000382:microbiology; D017354:Point Mutation; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D017434:Protein Structure, Tertiary; D027861:Prunus / Q000382:microbiology; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014774:Virulence / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D005659:Fungicides, Industrial; D006827:Hydantoins; D000620:Aminoimidazole Carboxamide; D011494:Protein Kinases; D000071677:Histidine Kinase; C033148:iprodione