Effect of postharvest storage on the expression of the apple allergen Mal d 1.
Consumption of fresh apples can cause allergy in susceptible individuals. A competitive enzyme-linked immunosorbent assay (ELISA) has been developed to determine Mal d 1 levels in apple pulp using a monoclonal antibody (BIP-1). The ELISA was able to rank ten cultivars according to their Mal d 1 content (between 3.8 and 72.5 mug/g pulp). For the first time, it has been demonstrated that growing conditions and postharvest storage, using three different treatments over a 5 month period in 2 consecutive years, increase Mal d 1 expression at a translational and transcriptional level (3.5- and 8.5-fold under controlled atmosphere storage). Expression of three major Mal d 1 isoforms was observed by real-time polymerase chain reaction over the 5 month storage period, and Mal d 1.02 was the most highly expressed isoform. In conclusion, Mal d 1 gene expression was significantly increased during modified atmosphere storage. Individuals suffering from birch pollen-apple allergy syndrome might experience fewer problems consuming freshly picked apples.
Sancho
Ana I
AI
Institute of Food Research, Norwich Research Park, Colney, UK. ana.sancho@bbsrc.ac.uk
Foxall
Robert
R
Browne
Tom
T
Dey
Rickmer
R
Zuidmeer
Laurian
L
Marzban
Gorji
G
Waldron
Keith W
KW
van Ree
Ronald
R
Hoffmann-Sommergruber
Karin
K
Laimer
Margit
M
Mills
E N Clare
EN
D000485:Allergens / Q000032:analysis* / Q000235:genetics; D052179:Antigens, Plant; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005519:Food Preservation / Q000379:methods*; D005638:Fruit / Q000737:chemistry / Q000276:immunology*; D015870:Gene Expression; D027845:Malus; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D015203:Reproducibility of Results; D013997:Time Factors
D000485:Allergens; D052179:Antigens, Plant; C095551:MALD1 protein, Malus domestica; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant