title: Usefulness of Toxoplasma gondii recombinant antigens (GRA1, GRA7 and SAG1) in an immunoglobulin G avidity test for the serodiagnosis of toxoplasmosis.
abstract: The precise diagnosis of an acute and recent Toxoplasma infection in pregnant women and the newborn child is important before treatment. This study describes a new Toxoplasma gondii IgG avidity test based on a combination of recombinant GRA1, GRA7 and SAG1 antigens and shows that this test is useful for diagnostic purposes and may replace the lysed, whole-cell antigens. Although more sera need to be tested, the results obtained here suggest that the IgG avidity test performed with rec-antigens correlated more with the stage of a T. gondii infection than the IgG avidity results obtained with the lysed, whole-cell antigen test, the VIDAS Toxo IgG avidity (bioMérieux).
authors.affiliation: Department of Tropical Parasitology, Inter-Faculty Institute of Maritime and Tropical Medicine, Medical University of Gdańsk, ul. Powstania Styczniowego 9b, Gdynia, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Pietkiewicz
authors.forename: E
authors.initials: E
authors.lastname: Hiszczyńska-Sawicka
authors.forename: J
authors.initials: J
authors.lastname: Kur
authors.forename: E
authors.initials: E
authors.lastname: Petersen
authors.forename: H V
authors.initials: HV
authors.lastname: Nielsen
authors.forename: M
authors.initials: M
authors.lastname: Paul
authors.forename: M
authors.initials: M
authors.lastname: Stankiewicz
authors.forename: P
authors.initials: P
authors.lastname: Myjak
mesh_terms: D000818:Animals; D000915:Antibody Affinity; D000953:Antigens, Protozoan / Q000276:immunology*; D007074:Immunoglobulin G; D015800:Protozoan Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D014122:Toxoplasma / Q000378:metabolism*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; C412913:GRA1 antigen; C112573:GRA7 protein, Toxoplasma gondii; D007074:Immunoglobulin G; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma