Developmental distribution of CaM kinase II in the antennal lobe of the sphinx moth Manduca sexta.
The antennal lobe (primary olfactory center of insects) is completely reorganized during metamorphosis. This reorganization is accompanied by changing patterns of calcium signaling in neurons and glial cells. In the present study, we investigated the developmental distribution of a major calcium-dependent protein, viz., calcium/calmodulin-dependent protein kinase II (CaM kinase II), in the antennal lobe of the sphinx moth Manduca sexta by using a monoclonal antibody. During synaptogenesis (developmental stages 6-10), we found a redistribution of CaM kinase II immunoreactivity, from a homogeneous distribution in the immature neuropil to an accumulation in the neuropil of the glomeruli. CaM kinase II immunoreactivity was less intense in olfactory receptor axons of the antennal nerve and antennal lobe glial cells. Western blot analysis revealed a growing content of CaM kinase II in antennal lobe tissue throughout metamorphosis. Injection of the CaM kinase inhibitor KN-93 into pupae resulted in a reduced number of antennal lobe glial cells migrating into the neuropil to form borders around glomeruli. The results suggest that CaM kinase II is involved in glial cell migration.
Lohr
Christian
C
Abteilung für Allgemeine Zoologie, Fachbereich Biologie, TU Kaiserslautern, Postfach 3049, 67653 Kaiserslautern, Germany. clohr@rhrk.uni-kl.de
Bergstein
Sandra
S
Hirnet
Daniela
D
D000818:Animals; D001596:Benzylamines / Q000494:pharmacology; D015153:Blotting, Western; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018402:Manduca / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D019581:Neuropil / Q000187:drug effects / Q000201:enzymology; D009833:Olfactory Pathways / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D011679:Pupa / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D013449:Sulfonamides / Q000494:pharmacology; D000096926:Benzenesulfonamides
D001596:Benzylamines; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D004791:Enzyme Inhibitors; D013449:Sulfonamides; C072105:KN 93; D000096926:Benzenesulfonamides