Cell-cell fusion as a means to establish pluripotency.
Embryonic stem cells (ESCs), embryonic germ cells (EGCs), and embryonic carcinoma cells (ECCs) are three types of pluripotent cells derived from mammalian embryos. The three cell types are capable not only of self-renewal, but also of having the potential to give rise to cells of all tissue types in the fetal and adult body. In several reports, ESCs, ECCs, and EGCs have been described to reprogram somatic cells in vitro. After reprogramming caused by fusion, somatic cells exhibit various features of pluripotent cells: expression of pluripotency markers (e.g., Oct4, nanog, and Rex-1), absence of tissue-specific gene expression, reactivation of inactive X chromosome of female somatic cells, demethylation, as well as histone modification. An activity in pluripotent stem cells appears to be capable of inducing the global changes inherent in the reprogramming of somatic cells. Investigations involving pluripotent stem cells will yield substantial insight into various fundamental biological processes, such as cellular differentiation and de-differentiation. Most importantly for the public, however, is that such studies might lead into cell-based therapies and as such have the potential to change regenerative medicine.
Do
J T
JT
Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, Münster, Germany. jeong.tae.do@mpi-muenster.mpg.de
Schöler
H R
HR
D000818:Animals; D002277:Carcinoma / Q000196:embryology / Q000235:genetics / Q000473:pathology; D002459:Cell Fusion; D044127:Epigenesis, Genetic; D016678:Genome / Q000235:genetics; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology*