title: Altered allergen-induced eosinophil trafficking and physiological dysfunction in airways with preexisting virus-induced injury.
abstract: Although both asthmatics and allergic rhinitics develop an acute inflammatory response to lower airway allergen challenge, only asthmatics experience airway obstruction resulting from chronic environmental allergen exposure. Hypothesizing that asthmatic airways have an altered response to chronic allergic inflammation, we compared the effects of repeated low-level exposures to inhaled Alternaria extract in sensitized rats with preexisting chronic postbronchiolitis airway dysfunction versus sensitized controls with normal airways. Measurements of air space (bronchoalveolar lavage) inflammatory cells, airway goblet cells, airway wall collagen, airway wall eosinophils, airway alveolar attachments, and pulmonary physiology were conducted after six weekly exposures to aerosolized saline or Alternaria extract. Postbronchiolitis rats, but not those starting with normal airways, had persistent increases in airway wall eosinophils, goblet cell hyperplasia in small airways, and loss of lung elastic recoil after repeated exposure to aerosolized Alternaria extract. Despite having elevated airway wall eosinophils, the postbronchiolitis rats had no eosinophils in bronchoalveolar lavage at 5 days after the last allergen exposure, suggesting altered egression of tissue eosinophils into the air space. In conclusion, rats with preexisting airway pathology had altered eosinophil trafficking and allergen-induced changes in airway epithelium and lung mechanics that were absent in sensitized control rats that had normal airways before the allergen exposures.
authors.affiliation: School of Pharmacy, Department of Medicine, University of Wisconsin at Madison, 777 Highland Ave, Madison, WI 53705, USA. rlsorkne@wisc.edu
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Sorkness
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Herricks
authors.forename: Renee J
authors.initials: RJ
authors.lastname: Szakaly
authors.forename: Robert F
authors.initials: RF
authors.lastname: Lemanske
authors.forename: Louis A
authors.initials: LA
authors.lastname: Rosenthal
mesh_terms: D000403:Airway Resistance; D000485:Allergens / Q000008:administration & dosage / Q000633:toxicity*; D000528:Alternaria / Q000276:immunology / Q000472:pathogenicity; D000818:Animals; D001990:Bronchiolitis, Viral / Q000150:complications / Q000503:physiopathology; D004804:Eosinophils / Q000473:pathology / Q000502:physiology*; D007249:Inflammation / Q000473:pathology; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D012140:Respiratory Tract Diseases / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D010253:Respirovirus Infections / Q000150:complications / Q000503:physiopathology; D029082:Sendai virus / Q000472:pathogenicity
chemical_list: D000485:Allergens