Production of D-arabitol by a metabolic engineered strain of Bacillus subtilis.
A novel method for D-arabitol production with a metabolically engineered Bacillus subtilis strain is described. A known transketolase-deficient and D-ribose-producing mutant of B. subtilis (ATCC 31094) was further modified by disruption of its rpi (D-ribose phosphate isomerase) gene to create a D-ribulose- and D-xylulose-producing B. subtilis strain. Expression of the D-arabitol phosphate dehydrogenase gene of Enterococcus avium in the D-ribulose- and D-xylulose-producing strain resulted in a strain of B. subtilis capable of converting D-glucose to D-arabitol with a high yield (38%) and little by-product formation.
Povelainen
Mira
M
Danisco Innovation, Sokeritehtaantie 20, 02460 Kantvik, Finland. mira.povelainen@danisco.com
Miasnikov
Andrei N
AN
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D016983:Enterococcus / Q000201:enzymology* / Q000235:genetics; D024861:Genetic Enhancement / Q000379:methods*; D005947:Glucose / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D013402:Sugar Alcohols / Q000302:isolation & purification / Q000378:metabolism*
D013402:Sugar Alcohols; C514286:D-arabitol phosphate dehydrogenase, Bacillus subtilis; D013401:Sugar Alcohol Dehydrogenases; D005947:Glucose; C014999:arabitol