[Association between polymorphisms of CYP17 and CYP3A4 genes and intrahepatic cholestasis of pregnancy in Chengdu].
OBJECTIVE
investigate whether polymorphisms in estrogen-metabolizing genes, CYP17 and CYP3A4, are associated with intrahepatic cholestasis of pregnancy (ICP) in Chengdu China.


METHODS
The -1931T/C polymorphism in the 5'-untranslated region of CYP17 gene and the -290A/G polymorphism in the 5'-regulatory region of CYP3A4 gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 100 pregnant women with ICP (ICP group) and 100 normal pregnant women (control group) in Chengdu.


RESULTS
The T/C polymorphism of the CYP17 gene was shown clearly in ICP and control groups, but the genotype distributions (TT, TC and CC) and allele frequencies (T and C) of CYP17 polymorphism were of no significant difference between the two groups (P > 0.05). No CYP3A4-V (CTP3A4 * 1B) allele was found in the 200 individuals studied. The genotype of CYP3A4 was wt/wt in all the pregnant women.


CONCLUSIONS
The T/C polymorphism of CYP17 gene is not associated with the risk of ICP in Chengdu. There are not -290A/G polymorphism of CYP3A4 gene among pregnunt women in Chengdu.
Zhang
Li
L
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Liu
Shu-yun
SY
Chen
Qiang
Q
Shi
Qing-yun
QY
Zou
Hai
H
Wu
Lin
L
D002681:China; D002780:Cholestasis, Intrahepatic / Q000235:genetics*; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D006801:Humans; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics*; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics*
D003577:Cytochrome P-450 Enzyme System; C104464:CYP3A protein, human; D051544:Cytochrome P-450 CYP3A; D013254:Steroid 17-alpha-Hydroxylase