Formation of GW bodies is a consequence of microRNA genesis.
GW bodies (GWBs), or mammalian P bodies, proposed to be involved in messenger RNA storage and/or degradation, have recently been linked to RNA interference and microRNA (miRNA) processing. We report that endogenous let-7 miRNA co-precipitates with the GW182 protein complex. In addition, knockdown of two proteins, Drosha and its protein partner DGCR8, which are vital to the generation of mature miRNA, results in the loss of GWBs. Subsequent introduction of short interference RNA specific to lamin A/C is accompanied by reassembly of GWBs and concurrent knockdown of lamin A/C protein. Taken together, these studies show that miRNAs are crucial components in GWB formation.
Pauley
Kaleb M
KM
Department of Oral Biology, University of Florida, 1600 SW Archer Road, PO Box 100424, Gainesville, 32610-0424, USA.
Eystathioy
Theophany
T
Jakymiw
Andrew
A
Hamel
John C
JC
Fritzler
Marvin J
MJ
Chan
Edward K L
EK
D022083:Cytoplasmic Structures / Q000378:metabolism* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism* / Q000502:physiology; D008954:Models, Biological; D020871:RNA Stability; D034443:RNA Transport; D043244:Ribonuclease III / Q000235:genetics*; D014162:Transfection
D035683:MicroRNAs; C517918:DROSHA protein, human; D043244:Ribonuclease III