Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins.
Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads.
Lee
In Su
IS
National Creative Research Initiative Center for Oxide Nanocrystalline Materials and School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea.
Lee
Nohyun
N
Park
Jongnam
J
Kim
Byung Hyo
BH
Yi
Yong-Weon
YW
Kim
Taeuk
T
Kim
Tae Kook
TK
Lee
In Hwan
IH
Paik
Seung R
SR
Hyeon
Taeghwan
T
D006639:Histidine / Q000737:chemistry*; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D006639:Histidine; D009532:Nickel; C028007:nickel monoxide