title: Experimental standards for high-throughput proteomics.
abstract: Proteome analysis, utilizing high-throughput proteomics approaches, involves studying proteins that a whole organism (or specific tissue or cellular compartment) expresses under certain conditions. Intrinsic difficulties of these studies, as well as the enormous volumes of data they typically produce, make the proteome analysis and interpretation very difficult. As with any high-throughput approach, proteomics experiments should be carefully designed, analyzed, and verified. In addition to computational standards,experimental standards--simple and complex mixtures of known proteins--for high-throughput proteomics have to be developed and utilized. This article discusses such experimental standards and their implementations.
authors.affiliation: The BIATECH Institute, Bothell, Washington 98011, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Hogan
authors.forename: Roger
authors.initials: R
authors.lastname: Higdon
authors.forename: Eugene
authors.initials: E
authors.lastname: Kolker
mesh_terms: D000818:Animals; D006801:Humans; D020543:Proteome / Q000032:analysis* / Q000592:standards*; D040901:Proteomics / Q000295:instrumentation / Q000592:standards*; D013673:Technology Assessment, Biomedical / Q000592:standards
chemical_list: D020543:Proteome