[HAI-1 expression in villous tissue of early gestational period in women].
OBJECTIVE
To survey the role of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in early embryo development, we study the HA-1 gene expressing in villous tissue after embryo implantation.


METHODS
Fifty villous tissue samples from pregnant 6 w to 10 w were divided into five groups and each group included ten samples. The HAI-1 gene and protein expressing in the villous tissue were detected by RT-PCR and immunohistochemistry.


RESULTS
The expression of HAI-1 had a gradual increase with the pregnancy weeks added. The HAl-1 gene expression was very low at pregnant 6 w(A(260/280) 0.3142), From pregnant 7 w to 9 w, the expression was increased gradually (A(260/280) 0.3476 in 7 w and 0.3910 in 8 w) and reached the pinnacle in 9 w (A(260/280) 0.4876). And then the pinnacle value lasted until 10 w (A(260/280) 0.4909). The proportion of HAI-1 mRNA/beta-actin mRNA between 9 w and 10 w was not significantly different (P > or = 0.05). But among the other 3 groups, the proportion was significantly different (P < 0.01). The proportion of 9 w and 10 w was significantly different from the other 3 groups (P < 0.01). The results of HAI-1 protein expression showed the similar conclusion by immunohistochemical analysis.


CONCLUSIONS
The HAI-1 gene may play an important role in early embryo development in human.
Fan
Cai-bo
CB
Department of Genetics, School of Public Health, Chongqing Medical University, Chongqing 400016, China.
He
Jun-lin
JL
Wang
Ying-xiong
YX
Liu
Xue-qing
XQ
Chen
Xue-mei
XM
Li
Hong-mei
HM
Ding
Yu-bin
YB
D000328:Adult; D002824:Chorionic Villi / Q000378:metabolism*; D005260:Female; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000378:metabolism*; D053491:Proteinase Inhibitory Proteins, Secretory; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D008562:Membrane Glycoproteins; D053491:Proteinase Inhibitory Proteins, Secretory; D012333:RNA, Messenger; C109149:SPINT1 protein, human