Histogranin reduced brain injury after transient focal ischemia in rats.
Excitatory amino acids (EAAs) play an important role during ischemic brain injury. In this study we examined the protective effect of histogranin (HN), an endogenous peptide that antagonizes excitatory amino acids-mediated activity noncompetitively, in an animal model of cerebral ischemia. Adult rats were anesthetized with chloral hydrate. Histogranin was given intracerebroventricularly before a 60-min middle cerebral artery occlusion (MCAo). Animals were examined for their locomotor activity 2 days after MCAo. Histogranin significantly increased locomotor activity in the stroke rats. Histogranin pretreatment reduced the volume of cerebral infarction and the caspase-3 immunoreactivity in the stroke animals. Taken together, our data suggest that histogranin is protective against ischemic brain injury. The protective effect may involve anti-apoptotic mechanisms.
Chiang
Y H
YH
Dept Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
Chen
G J
GJ
Shen
H
H
Chou
J
J
Wang
Y
Y
D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal; D001930:Brain Injuries / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D004195:Disease Models, Animal; D018691:Excitatory Amino Acid Antagonists / Q000627:therapeutic use*; D007839:Functional Laterality; D002546:Ischemic Attack, Transient / Q000150:complications; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D011506:Proteins / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013778:Tetrazolium Salts
D018691:Excitatory Amino Acid Antagonists; D011506:Proteins; D013778:Tetrazolium Salts; C082312:histogranin; C009591:triphenyltetrazolium; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases