New nonradioactive technique for vasoactive intestinal peptide-receptor-ligand-binding studies.
We describe fluorescent-labeled peptide (FLP) studies on living cells. The new technique is nonradioactive and it allows monitoring of the binding and internalization of Vasoactive Intestinal Peptide (VIP) in VIP receptor-expressing cells. The technique is easy to perform and the observed reaction is peptide sequence specific.
Haberl
Ines
I
Department of Internal Medicine I, Division: Institute of Cancer Research, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, Austria.
Habringer
Daniela Susanne
DS
Andreae
Fritz
F
Artl
Andreas
A
Mosgoeller
Wilhelm
W
D002232:Carbocyanines / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D008024:Ligands; D008856:Microscopy, Fluorescence / Q000379:methods*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D011485:Protein Binding; D011869:Radioligand Assay; D018005:Receptors, Vasoactive Intestinal Peptide / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000737:chemistry / Q000378:metabolism*
D002232:Carbocyanines; D008024:Ligands; D018005:Receptors, Vasoactive Intestinal Peptide; C089187:cyanine dye 3; D014660:Vasoactive Intestinal Peptide