title: Functional genomics: applying calcium imaging and RNA interference to Drosophila cell lines to identify new roles for gene products.
abstract: The selective knockdown of genes using RNA interference, and the analysis of the resultant phenotype using calcium imaging, is proving to be a powerful combination for discovering new genes involved in calcium signalling. This technical note describes the application of this approach to the Drosophila S2 cell line and its derivatives.
authors.affiliation: MRC Functional Genetics Unit, Department of Physiology, Anatomy and Genetics Le Gros Clark Building, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK. steven.buckingham@human-anatomy.oxford.ac.uk
authors.forename: Steven D
authors.initials: SD
authors.lastname: Buckingham
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003952:Diagnostic Imaging; D004330:Drosophila / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D008264:Macrophages / Q000378:metabolism*; D034622:RNA Interference
chemical_list: D002118:Calcium