Evaluation of compliance and stiffness of decellularized tissues as scaffolds for tissue-engineered small caliber vascular grafts using intravascular ultrasound.
This study evaluated the compliance and stiffness of decellularized canine common carotid artery as well as decellularized canine ureter and compared it with that of polytetrafluoroethylene, elastin gel combined with polylactic acid tube, and canine common carotid artery. To calculate the compliance and stiffness, internal diameters and cross-sectional areas were measured according to changes in the intraluminal pressures using intravascular ultrasound in a closed circuit system equipped with a syringe pump. The pressure-area curve, stiffness parameter beta, and diameter compliance were evaluated. Canine common carotid artery and decellularized canine common carotid artery, as well as decellularized canine ureter, showed a compliant response, a J-shaped curve. However, the latter evidenced different characteristics in the low pressure range. Although the cross-sectional area of the elastin gel combined with polylactic acid tube showed some changes, it did not present a J-shape curve. Polytetrafluoroethylene exhibited a noncompliant response.The results in this study have shown that the compliance in the decellularized matrices was maintained after cell extraction, which demonstrated the importance of the remaining matrix structure in the mechanical properties of decellularized tissue. A clear difference between the decellularized matrices and synthetic materials was noted in terms of the compliance, even in materials composed of relatively elastic materials.
Murase
Yosuke
Y
Department of Cardiology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Narita
Yuji
Y
Kagami
Hideaki
H
Miyamoto
Keiichi
K
Ueda
Yuichi
Y
Ueda
Minoru
M
Murohara
Toyoaki
T
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D001673:Biodegradation, Environmental; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D017536:Carotid Artery, Common / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000637:transplantation; D003187:Compliance; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004285:Dogs; D004548:Elasticity; D004549:Elastin / Q000737:chemistry; D005069:Evaluation Studies as Topic; D005782:Gels / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D036103:Nanotechnology; D011091:Polyesters; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering; D014465:Ultrasonics; D014463:Ultrasonography; D014513:Ureter / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000637:transplantation
D001672:Biocompatible Materials; D003902:Detergents; D005782:Gels; D011091:Polyesters; D011093:Polyethylene Terephthalates; D011108:Polymers; D003840:Deoxycholic Acid; D019344:Lactic Acid; C033616:poly(lactide); D004549:Elastin