title: [Study in vitro on p16beta interfering the cell cycle signal conduction of laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the effect of p16beta interfering with the signal conduction of Hep-2 cell cycle in Vitro.
  
  
  METHODS
  AdEasy Vector System was used to construct the recombinant adenovirus vector AdEasy-GFP-p1613. The recombinant adenovirus vector could introduce p16beta gene into HEK 293 cell. Then the purified recombinant adenovirus was used to infect Hep-2 cells in vitro. The protein expression of p16beta, proliferation inhibition, cell cycle arrest, DNA content, apoptosis ratio in Hep-2 cells were examined by MTT, Western blotting analysis, Flow cytometry assay, Immunocytochemistry respectively.
  
  
  RESULTS
  The recombinant adenovirus Adeasy-p16beta was constructed successfully and higher titer recombinant adenovirus particle was got. When Adeasy-p16beta was transferred into Hep-2 cells, both the growth inhibition and P14 (ARF) protein overexpression in Hep-2 cells were visible effectively. The most Hep-2 cells were blocked in G1/G0 phase and the cell apoptosis ratio increased.
  
  
  CONCLUSIONS
  The p16beta in Hep-2 cell infected by recombinant adenovirus, which lead to express P14 (ARF) protein effectively and to inhibit cell proliferative activation, effectively interfere the signal conduction mechanisms of culture Hep-2 laryngeal squamous cell carcinoma, and the growth of much cultured cells is blocked in G1/G0 phase.
authors.affiliation: Department of Otolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Min
authors.initials: M
authors.lastname: Fan
authors.forename: Jun-ming
authors.initials: JM
authors.lastname: Xian
authors.forename: Guang-yao
authors.initials: GY
authors.lastname: Zhou
authors.forename: Chuan-yu
authors.initials: CY
authors.lastname: Liang
authors.forename: Shi-xi
authors.initials: SX
authors.lastname: Liu
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002453:Cell Cycle; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D015398:Signal Transduction; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16