[Effect of jiangu granule on levels of urinary deoxypyridinoline and osseous intigrin beta1 mRNA expression in Ovariectomized osteoporosis model rats].
OBJECTIVE
To investigate the regulatory effect of Jiangu Granule (JGG) on osteoblast and its effect on bone absorption on the molecular level and from aspect of bone metabolism.


METHODS
Osteoporosis model SD rats established by ovariectomy were interfered by drug. Levels of urinary deoxypyridinoline (D-Pyr) and osseous integrin beta1 (Itgbeta1) mRNA expression were detected by ELISA and in situ hybridization, respectively.


RESULTS
Level of urinary D-Pyr increased (P < 0.01) and osseous Itgbeta1 mRNA expression decreased significantly in the model rats. After treatment with JGG for 12 weeks, the D-Pyr levels in them decreased significantly (P < 0.05), while the quantity of osteoblasts and the expression of Itgbeta1 mRNA in bone tissue increased obviously. Conclusion JGG can not only decrease the catabolism of collagen fiber type I in bone matrix, but also promote the osteogenic activity of osteoblast. So, it can improve or reverse the pathological tendency of osteogenesis shortage caused by E2 reduction to play its action in strengthening bone.
Lin
Yan-ping
YP
Faculty of Orthopedics and Traumatology, Fujian College of TCM, Fuzhou. lyp66@126.com
Zhou
Rui-xiang
RX
Li
Yi-long
YL
D048868:Adaptor Proteins, Signal Transducing; D000596:Amino Acids / Q000652:urine*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010024:Osteoporosis / Q000378:metabolism* / Q000517:prevention & control; D010052:Ovariectomy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D048868:Adaptor Proteins, Signal Transducing; D000596:Amino Acids; D004365:Drugs, Chinese Herbal; C114243:Itgb1bp1 protein, mouse; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C036020:deoxypyridinoline