title: Broad-range polymerase chain reaction for the diagnosis of Bartonella henselae endocarditis.
abstract: We present a case of subacute bacterial endocarditis in a 10-year-old girl with Di-George syndrome, congenital heart disease, and mild immunodeficiency. She was afebrile at initial presentation but was found to have massive splenomegaly, and signs of congestive heart failure. No causative organism could be identified on routine blood and tissue cultures. A detailed clinical history revealed a history that she had been scratched by a cat and developed intermittant fevers over 3 months. Bartonella henselae was identified by broad-range 16S r-DNA polymerase chain reaction on valvular tissue specimens.
authors.affiliation: Department of Infectious Diseases, Great Ormond Street Hospital for Children, London, UK. wallst@gosh.nhs.uk
authors.forename: Tony
authors.initials: T
authors.lastname: Walls
authors.forename: Karyn
authors.initials: K
authors.lastname: Moshal
authors.forename: John
authors.initials: J
authors.lastname: Trounce
authors.forename: John
authors.initials: J
authors.lastname: Hartley
authors.forename: Kathryn
authors.initials: K
authors.lastname: Harris
authors.forename: Graham
authors.initials: G
authors.lastname: Davies
mesh_terms: D018416:Bartonella henselae / Q000235:genetics*; D002648:Child; D004697:Endocarditis, Bacterial / Q000175:diagnosis* / Q000188:drug therapy; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S; D006113:United Kingdom
chemical_list: D012336:RNA, Ribosomal, 16S