[Antibodies against heat shock proteins (HSP) in children with chronic renal failure (CRF)--preliminary results].
Current data suggest the role of autoimmune reactions in the pathogenesis of cardiovascular complications in CRF patients. Elevated levels of anti-Hsp autoantibodies (anti-Hsp60, anti-Hsp70) have been described in adults with atherosclerotic lesions and cardiovascular events. The aim of the study was to evaluate the levels of anti-Hsp60 and anti-Hsp70 in sera of CRF children. Serum uric acid, lipid profile, erythrocyte sedimentation rate (ESR) and hsCRP were also estimated.


METHODS
The study was performed on 18 CRF children treated conservatively, divided into 2 subgroups: 8 patients with serum creatinine levels below 265 micromol/l (gr. I) and 10 patients with serum creatinine levels above 265 micromol/l (gr. II). The control group consisted of 13 healthy age-matched subjects with normal kidney function. Anti-Hsp concentrations were evaluated by ELISA, hsCRP levels--by nephelometry.


RESULTS
There were no differences in median values of anti-Hsp60, anti-Hsp70 and total cholesterol (CHOL) between examined groups. Mean hsCRP levels were also comparable. No difference was seen when HDL-C and LDL-C levels in gr. I and control group were compared, whereas TGL concentrations were elevated in gr.I vs. controls (p<0.001). In gr. II HDL-C levels were lower vs. controls (p<0.01), whereas LDL-C and TGL concentrations were higher compared to controls (p<0.001, p<0.0001). LDL-C levels in gr.II were also elevated vs. gr. I (p<0.01). Anti-Hsp70 correlated with serum uric acid (R=-0.82, p=0.04), cholesterol (R=-0.78, p=0.03), CHOL/LDL-C (R=-0.89, p=0.007) and CHOL/TGL (R=-0.75, p=0.03) in gr. I and with ESR after 1h in gr. II (R=0.72, p=0.03). No correlations were found between hsCRP and other parameters.


CONCLUSIONS
Preliminary results point at relations between anti-Hsp70, markers of chronic inflammation (ESR) and atherosclerotic risk (uric acid, cholesterol) in CRF children.
Musiał
Kinga
K
Katedra i Klinika Nefrologii Pediatrycznej, Akademii Medycznej im Piastów Slaskich we Wrocławiu. kinga_musial@hotmail.com
Zwolińska
Danuta
D
Polak-Jonkisz
Dorota
D
Makulska
Irena
I
Miler
Maria
M
D000293:Adolescent; D001323:Autoantibodies / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000097:blood* / Q000276:immunology*; D006801:Humans; D007223:Infant; D007676:Kidney Failure, Chronic / Q000097:blood / Q000276:immunology* / Q000652:urine; D007677:Kidney Function Tests; D008055:Lipids; D008297:Male; D014280:Triglycerides / Q000097:blood*
D001323:Autoantibodies; D018840:HSP70 Heat-Shock Proteins; D008055:Lipids; D014280:Triglycerides