Detecting nucleosome ladders on unique DNA sequences in mouse liver nuclei.
Nucleosome arrangements and possibly chromatin higher order structures differ in different regions of genomic DNA, and these differences could be functionally important. Nucleosome arrangements are reflected by the nucleosome ladders they give rise to upon micrococcal nuclease digestion of the chromatin. Here we describe how Southern hybridization can be used to detect the nucleosome ladders arising from different regions of DNA in mouse liver nuclei.
Fleury
Tomara J
TJ
Department of Biological Sciences, Purdue University, West Lafayette, IN, USA.
Cioffi
Alfred
A
Stein
Arnold
A
D000818:Animals; D015139:Blotting, Southern / Q000379:methods*; D002458:Cell Fractionation; D002843:Chromatin / Q000235:genetics; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D008099:Liver / Q000378:metabolism*; D051379:Mice; D015335:Molecular Probes; D009707:Nucleosomes / Q000235:genetics*; D010761:Phosphorus Radioisotopes; D016133:Polymerase Chain Reaction
D002843:Chromatin; D015335:Molecular Probes; D009707:Nucleosomes; D010761:Phosphorus Radioisotopes; D004247:DNA