Chryseobacterium taiwanense sp. nov., isolated from soil in Taiwan.
Among a large collection of Taiwanese soil isolates, a novel Gram-negative, rod-shaped, non-spore-forming, yellow-pigmented bacterial strain, Soil-3-27(T), was isolated from farmland soil in Wu-Feng, Taiwan. The isolate was subjected to a polyphasic study including 16S rRNA gene sequencing, DNA-DNA hybridization, fatty acid analysis and comparative phenotypic characterization. The 16S rRNA gene sequence analysis indicated that the organism belongs to the genus Chryseobacterium. The organism contains menaquinone MK-6 as the predominant isoprenoid quinone and 15 : 0 iso (43 %), 17 : 1 isoomega9c (17.5 %) and 17 : 0 iso 3-OH (16.6 %) as the major fatty acids. Phylogenetically, the closest relatives of strain Soil-3-27(T) are Chryseobacterium daecheongense, Chryseobacterium defluvii and Chryseobacterium taichungense with 96.7-97.2 % sequence similarity. DNA-DNA hybridization showed relatedness values of 8.5-24.2 % with these species. The DNA G+C content is 36.8 mol%. Strain Soil-3-27(T) is clearly distinguishable from other Chryseobacterium species and represents a novel species, for which the name Chryseobacterium taiwanense sp. nov. is proposed. The type strain is strain Soil-3-27(T) (=BCRC 17412(T)=IAM 15317(T)=LMG 23355(T)).
Tai
Chun-Ju
CJ
Bioresource Collection and Research Center, Food Industry Research and Development Institute, PO Box 246, Hsinchu 30062, Taiwan.
Kuo
Hsiao-Ping
HP
Bioresource Collection and Research Center, Food Industry Research and Development Institute, PO Box 246, Hsinchu 30062, Taiwan.
Lee
Fwu-Ling
FL
Bioresource Collection and Research Center, Food Industry Research and Development Institute, PO Box 246, Hsinchu 30062, Taiwan.
Chen
Han-Ken
HK
Bioresource Collection and Research Center, Food Industry Research and Development Institute, PO Box 246, Hsinchu 30062, Taiwan.
Yokota
Akira
A
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo 113-0032, Japan.
Lo
Chi-Chu
CC
Division of Bio-Pesticide, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Taiwan.
D001482:Base Composition; D045247:Chryseobacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013045:Species Specificity; D013624:Taiwan; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2