title: Protein translocation by bacterial toxin channels: a comparison of diphtheria toxin and colicin Ia.
abstract: Regions of both colicin Ia and diphtheria toxin N-terminal to the channel-forming domains can be translocated across planar phospholipid bilayer membranes. In this article we show that the translocation pathway of diphtheria toxin allows much larger molecules to be translocated than does the translocation pathway of colicin Ia. In particular, the folded A chain of diphtheria toxin is readily translocated by that toxin but is not translocated by colicin Ia. This difference cannot be attributed to specific recognition of the A chain by diphtheria toxin's translocation pathway because the translocation pathway also accommodates folded myoglobin.
authors.affiliation: Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Wu
authors.forename: Karen S
authors.initials: KS
authors.lastname: Jakes
authors.forename: Ben S
authors.initials: BS
authors.lastname: Samelson-Jones
authors.forename: Bing
authors.initials: B
authors.lastname: Lai
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Erwin
authors.initials: E
authors.lastname: London
authors.forename: Alan
authors.initials: A
authors.lastname: Finkelstein
mesh_terms: D003087:Colicins / Q000737:chemistry*; D004167:Diphtheria Toxin / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D021381:Protein Transport
chemical_list: D003087:Colicins; D004167:Diphtheria Toxin; D008051:Lipid Bilayers; D009211:Myoglobin; D010743:Phospholipids