Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicus.
A restriction endonuclease activity from Sulfolobus islandicus REN2H1 was purified by phosphocellulose and cation exchange chromatography. The enzyme cuts DNA at the recognition site GCwGC as could be shown by restriction analysis of plasmids and short synthetic duplex DNA. The cleavage occurs after the first guanosine base and is inhibited by 5-methyl-cytosine methylation. The restriction activity is salt-sensitive and has an optimal activity around 70 degrees C.
Söllner
Stefan
S
Institute of Biochemistry, University of Bayreuth, Universitätstrasse 30, 95447 Bayreuth, Germany.
Berkner
Silvia
S
Lipps
Georg
G
D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002411:Cation Exchange Resins; D002482:Cellulose / Q000031:analogs & derivatives; D002852:Chromatography, Ion Exchange / Q000379:methods; D003596:Cytosine / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D019175:DNA Methylation; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011489:Protein Denaturation; D012965:Sodium Chloride / Q000737:chemistry; D016944:Sulfolobus / Q000201:enzymology*; D013696:Temperature
D019843:Archaeal Proteins; D002411:Cation Exchange Resins; D012965:Sodium Chloride; D003596:Cytosine; D002482:Cellulose; D004247:DNA; C008881:phosphocellulose; D015252:Deoxyribonucleases, Type II Site-Specific