title: PACAP stimulates the release of the secretogranin II-derived peptide EM66 from chromaffin cells.
abstract: The aim of the present article was to examine the effect of PACAP on the release of the SgII-derived peptide EM66 from primary cultures of bovine chromaffin cells. PACAP dose dependently stimulated EM66 release from cultured chromaffin cells. A significant response was observed after 6 h of treatment with PACAP and increased to reach a 3.6-fold stimulation at 72 h. The stimulatory effect of PACAP was mediated through multiple signaling pathways, including calcium influx through L-type channels, PKA, PKC, and MAP-kinase cascades, to regulate EM66 release from chromaffin cells. These data suggest that EM66 may act downstream of the trans-synaptic stimulation of the adrenal medulla by neurocrine factors.
authors.affiliation: INSERM U413, Laboratory of Cellular and Molecular Neuroendocrinology, European Institute for Peptide Research (IFRMP23), University of Rouen, 76821 Mont-Saint-Aignan, France.
authors.forename: Johann
authors.initials: J
authors.lastname: Guillemot
authors.forename: Djida
authors.initials: D
authors.lastname: Ait-Ali
authors.forename: Valerie
authors.initials: V
authors.lastname: Turquier
authors.forename: Maite
authors.initials: M
authors.lastname: Montero-Hadjadje
authors.forename: Alain
authors.initials: A
authors.lastname: Fournier
authors.forename: Hubert
authors.initials: H
authors.lastname: Vaudry
authors.forename: Youssef
authors.initials: Y
authors.lastname: Anouar
authors.forename: Laurent
authors.initials: L
authors.lastname: Yon
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000187:drug effects* / Q000378:metabolism*; D002864:Chromogranins / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000494:pharmacology*; D053381:Secretogranin II / Q000494:pharmacology*
chemical_list: D002864:Chromogranins; C557436:EM66 peptide; D010446:Peptide Fragments; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D053381:Secretogranin II