Characterization of equid herpesvirus 1 (EHV-1) related viruses from captive Grevy's zebra and blackbuck.
Equid herpes virus 1 (EHV-1) related isolates from a captive blackbuck (strain Ro-1) and Grevy's zebra (strain T965) behaved similarly to EHV-1 and EHV-9 in respect to their host cell range. Restriction enzyme analysis and a phylogenetic tree confirmed that Ro-1 and T965 were identical and more closely related to EHV-1 than to EHV-9. Differences from EHV-1 became obvious firstly, by amino acid alignments revealing two unique substitutions in the gB protein of Ro-1 and T965. Secondly, an EHV-1 type-specific monoclonal antibody did not detect its antigen on Ro-1, T965 or EHV-9 infected cells by immunohistochemistry. The results support the view that Ro-1 and T965 isolates represent a distinct, previously unrecognized species of equid herpesviruses.
Borchers
Kerstin
K
Institut für Virologie, FU Berlin, Königin-Luise-Str., Berlin, Germany.
Böttner
Daniel
D
Lieckfeldt
Dietmar
D
Ludwig
Arne
A
Frölich
Kai
K
Klingeborn
Berndt
B
Widèn
Frederik
F
Allen
George
G
Ludwig
Hanns
H
D000595:Amino Acid Sequence; D000818:Animals; D000866:Antelopes / Q000821:virology*; D018397:Equidae / Q000821:virology*; D004861:Herpesvirus 1, Equid / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D014764:Viral Proteins