title: Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship.
abstract: An expressed sequence tag library has been generated from a sand fly vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed from whole adults and 16,608 clones were sequenced from both ends and assembled into 10,203 contigs and singlets. Of these 58% showed significant similarity to known genes from other organisms, <4% were identical to described sand fly genes, and 42% had no match to any database sequence. Our analyses revealed putative proteins involved in the barrier function of the gut (peritrophins, microvillar proteins, glutamine synthase), digestive physiology (secreted and membrane-anchored hydrolytic enzymes), and the immune response (gram-negative binding proteins, thioester proteins, scavenger receptors, galectins, signaling pathway factors, caspases, serpins, and peroxidases). Sequence analysis of this transcriptome dataset has provided new insights into genes that might be associated with the response of the vector to the development of Leishmania.
authors.affiliation: Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK. Electronic address: r.j.dillon@liv.ac.uk.
authors.forename: Rod J
authors.initials: RJ
authors.lastname: Dillon
authors.affiliation: The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: Al C
authors.initials: AC
authors.lastname: Ivens
authors.affiliation: The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: Carol
authors.initials: C
authors.lastname: Churcher
authors.affiliation: The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: Nancy
authors.initials: N
authors.lastname: Holroyd
authors.affiliation: The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Quail
authors.affiliation: Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Rogers
authors.affiliation: Children's Memorial Research Center and Northwestern University, Chicago, IL 60611, USA.
authors.forename: M Bento
authors.initials: MB
authors.lastname: Soares
authors.affiliation: Children's Memorial Research Center and Northwestern University, Chicago, IL 60611, USA.
authors.forename: Maria F
authors.initials: MF
authors.lastname: Bonaldo
authors.affiliation: Department of Electrical and Computer Engineering, University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Casavant
authors.affiliation: Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK.
authors.forename: Mike J
authors.initials: MJ
authors.lastname: Lehane
authors.affiliation: Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Bates
mesh_terms: D000818:Animals; D019295:Computational Biology; D020224:Expressed Sequence Tags; D006790:Host-Parasite Interactions; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007891:Leishmania / Q000502:physiology*; D008969:Molecular Sequence Data; D011576:Psychodidae / Q000235:genetics* / Q000469:parasitology*; D017422:Sequence Analysis, DNA
chemical_list: D019476:Insect Proteins