[Screen of media for insect cells (HzAm1) growth and its adaptation to low serum culture].
Insect cell-baculovirus system is a useful tool for both insecticidal virus production and the expression of medically useful foreign genes. Serum-free culture or low serum culture for insect cells is essential and significant. Media for the growth of insect cells HzAm1 from three kinds of commercial media TC-100,GRACE and IPL-41 with 10% serum were investigated and screened. The result shows that medium TC-100 is the most suitable medium for the growth of cells HzAm1. Adaptation of insect cells HzAm1 to cultures in medium TC-100 with serum reduction from 10% to 1% was carried out as cultures were supplemented with lactalbumin hydrolysate and yeastolate etc. Growth of insect cells HzAml in medium TC-100 with serum 1%, lactalbumin hydrolysate and yeastolate was well.
Zhang
You-Hong
YH
Hubei Key Laboratory of Novel Chemical Reactor & Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, China. youhong64@yahoo.com.cn
Wang
Hua-Lin
HL
Zhu
Xiong-Wei
XW
Qin
Qin
Q
Chen
Yan
Y
Lü
Zhong
Z
Ma
Jie
J
Zhang
Jing
J
D000222:Adaptation, Physiological; D000818:Animals; D049109:Cell Proliferation; D003470:Culture Media; D016895:Culture Media, Serum-Free; D007915:Lepidoptera / Q000166:cytology*
D003470:Culture Media; D016895:Culture Media, Serum-Free