[Molecular dynamics modeling of the substitution of serine for the conservative glycine in the G loop in the yeast cdc28-srm mutant using the crystalline lattice of human kinase CDK2].
Two-nanosecond molecular dynamics modeling of the crystalline lattice of an active complex of kinase pT160-CDK2/cyclin A/ATP-Mg2+ substrate has been performed. The results of modeling indicated that the structures of the nonmutant CDK2 complex and mutant CDK2 complex, which involves the G 16S-CD K2 substitution corresponding to that of yeast, markedly differ, the differences in structural conformations being particularly well pronounced in those regions that play a key role in the functioning of kinase. Based on the results of computations, structural elements are considered that may affect the kinase activity and regulatory phosphorylation, and the binding of protein kinase to cyclins and substrates.
Kholmurodov
Kh T
KhT
Kretov
D A
DA
Gerasimova
A S
AS
Koltovaia
N A
NA
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D017489:CDC28 Protein Kinase, S cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008274:Magnesium / Q000737:chemistry; D008958:Models, Molecular; D017354:Point Mutation; D011485:Protein Binding / Q000235:genetics; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D000255:Adenosine Triphosphate; D017489:CDC28 Protein Kinase, S cerevisiae; D051357:Cyclin-Dependent Kinase 2; D008274:Magnesium