title: Proliferation and osteoblastic differentiation of human bone marrow-derived stromal cells on akermanite-bioactive ceramics.
abstract: In the present study, the effects of a calcium magnesium silicate bioactive ceramic (akermanite) on proliferation and osteoblastic differentiation of human bone marrow stromal cells (hBMSC) have been investigated and compared with the classical ceramic (beta-tricalcium phosphate, beta-TCP). Akermanite and beta-TCP disks were seeded with hBMSC and kept in growth medium or osteogenic medium for 10 days. Proliferation and osteoblastic differentiation were evaluated on day 1, 4, 7 and 10. The data from the Alamar Blue assay and lactic acid production assay showed that hBMSC proliferated more significantly on akermanite than on beta-TCP. The analysis of osteoblast-related genes, including alkaline phosphatase (ALP), osteopontin (OPN), bone sialoprotein (BSP) and osteocalcin (OC), indicated that akermanite ceramics enhanced the expression of osteoblast-related genes, but type I collagen (COL I) showed no noticeable difference among akermanite and beta-TCP ceramics. Furthermore, this stimulatory effect was observed not only in osteogenic medium, but also in normal growth medium without osteogenic reagents such as l-ascorbic acid, glycerophosphate and dexamethasone. This result suggests that akermanite can promote osteoblastic differentiation of hBMSC in vitro even without osteogenic reagents, and may be used as a bioactive material for bone regeneration and tissue engineering applications.
authors.affiliation: Orthopaedic Cellular & Molecular Biology Laboratory, Laboratory of Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences & Shanghai JiaoTong University School of Medicine, People's Republic of China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Sun
authors.forename: Chengtie
authors.initials: C
authors.lastname: Wu
authors.forename: Kerong
authors.initials: K
authors.lastname: Dai
authors.forename: Jiang
authors.initials: J
authors.lastname: Chang
authors.forename: Tingting
authors.initials: T
authors.lastname: Tang
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002130:Calcium Phosphates / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics / Q000378:metabolism*; D003764:Dental Materials / Q000378:metabolism; D006801:Humans; D008297:Male; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D002130:Calcium Phosphates; D003764:Dental Materials; C485817:beta-tricalcium phosphate; C023506:Glass ceramics; D000469:Alkaline Phosphatase