A novel mode of tangential migration of cortical projection neurons.
Projection neurons of the developing cerebral cortex are generated in the cerebral ventricular zone and subsequently move to the developing cortical plate via radial migration. Conversely, most inhibitory interneurons originate in the ganglionic eminences and enter the developing cortical plate by tangential migration. Using immunohistochemical analysis together with tracer labeling experiments in organotypic brain slices, we show that a portion of cortical projection neurons migrates tangentially over long distances. Lineage analysis revealed that these neurons are derived from Emx1+ cortical progenitors and express the transcription factor Satb2 but do not express GABA or Olig1. In vitro and in vivo analysis of reeler mutant brains demonstrated that although reeler mutation does not influence tangential migration of interneurons, it affects the tangential migration of cortical projection neurons.
Britanova
Olga
O
Department of Molecular Biology of Neuronal Signals, Max-Plank-Institute for Experimental Medicine, 37075 Göttingen, Germany.
Alifragis
Pavlos
P
Junek
Stephan
S
Jones
Kevin
K
Gruss
Peter
P
Tarabykin
Victor
V
D000818:Animals; D001921:Brain / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics; D002465:Cell Movement; D002540:Cerebral Cortex / Q000166:cytology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics; D006624:Hippocampus / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics; D036961:Matrix Attachment Region Binding Proteins / Q000378:metabolism*; D051379:Mice; D008818:Mice, Neurologic Mutants; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000235:genetics; D009474:Neurons / Q000502:physiology*; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D009924:Organ Culture Techniques; D020671:Posterior Horn Cells / Q000378:metabolism; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000099260:Empty Spiracles Homeobox Proteins
D015816:Cell Adhesion Molecules, Neuronal; D004268:DNA-Binding Proteins; D016326:Extracellular Matrix Proteins; D018398:Homeodomain Proteins; D036961:Matrix Attachment Region Binding Proteins; D009419:Nerve Tissue Proteins; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D000091042:Reelin Protein; D012697:Serine Endopeptidases; D014157:Transcription Factors; D000099260:Empty Spiracles Homeobox Proteins; C539883:Nr4a2 protein, mouse; C501473:SATB2 protein, mouse