title: Artificial ribonucleases: from combinatorial libraries to efficient catalysts of RNA cleavage.
abstract: Combinatorial libraries of small organic compounds capable of cleaving RNA were synthesized. The compounds contain benzene ring substituted with two residues of bis quaternary salt of diazabicyclo[2.2.2]octane (DABCO) bearing hydrophobic fragments of different length and structure, attached to DABCO at the bridge position. These compounds, lacking traditional functionalities involved in transesterification reaction, exhibit pronounced RNA cleavage activity. To identify the most active artificial ribonucleases, sublibraries and truncated libraries, containing compounds lacking one of substituents were synthesized. Analysis of ribonuclease activity of truncated libraries resulted in identification of the most active compounds, which are characterized by the presence of at least one long oligomethylene substituent.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, 8, Lavrentiev Ave., Novosibirsk, 630090, Russian Federation.
authors.forename: N
authors.initials: N
authors.lastname: Kovalev
authors.forename: E
authors.initials: E
authors.lastname: Burakova
authors.forename: V
authors.initials: V
authors.lastname: Silnikov
authors.forename: M
authors.initials: M
authors.lastname: Zenkova
authors.forename: V
authors.initials: V
authors.lastname: Vlassov
mesh_terms: D000473:Alkanes / Q000737:chemistry; D001555:Benzene Derivatives / Q000138:chemical synthesis / Q000737:chemistry; D002384:Catalysis; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D009843:Oligoribonucleotides / Q000737:chemistry; D010879:Piperazines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000473:Alkanes; D001555:Benzene Derivatives; D009843:Oligoribonucleotides; D010879:Piperazines; D012313:RNA; D012260:Ribonucleases; C007306:triethylenediamine