Quantitative in vivo microscopy: the return from the 'omics'.
The confluence of recent advances in microscopy instrumentation and image analysis, coupled with the widespread use of GFP-like proteins as reporters of gene expression, has opened the door to high-throughput in vivo studies that can provide the morphological and temporal context to the biochemical pathways regulating cell function. We are now able to quantify the concentration and three-dimensional distribution of multiple spectrally resolved GFP-tagged proteins. Using automatic segmentation and tracking we can then measure the dynamics of the processes in which these elements are involved. In this way, parallel studies are feasible where multiple cell colonies treated with drugs or gene expression repressors can be monitored and analyzed to study the dynamics of relevant biological processes.
Fernández-González
Rodrigo
R
Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA.
Muñoz-Barrutia
Arrate
A
Barcellos-Hoff
Mary Helen
MH
Ortiz-de-Solorzano
Carlos
C
D000818:Animals; D001695:Biology / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D053858:Metabolic Networks and Pathways / Q000235:genetics / Q000502:physiology; D008856:Microscopy, Fluorescence / Q000379:methods*
D049452:Green Fluorescent Proteins