A genomewide screen for components of the RNAi pathway in Drosophila cultured cells.
Posttranscriptional silencing by RNAi is initiated by dsRNAs that are processed into siRNAs that ultimately target homologous mRNAs for degradation. We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway in Drosophila melanogaster. The screen identified seven genes that affect the RNAi response, five with previously described function (AGO2, Tis11, Hsc70-3, Hsc70-4, and hdc) and two annotated genes (CG17265 and CG10883).
Dorner
Silke
S
Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Lum
Lawrence
L
Kim
Michelle
M
Paro
Renato
R
Beachy
Philip A
PA
Green
Rachel
R
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004331:Drosophila melanogaster; D020868:Gene Silencing; D017344:Genes, Insect; D005821:Genetic Techniques; D016678:Genome; D008156:Luciferases / Q000378:metabolism; D008957:Models, Genetic; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D012330:RNA, Double-Stranded / Q000737:chemistry; D034741:RNA, Small Interfering / Q000378:metabolism
D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D008156:Luciferases