[Application of monoclonal antibody immobilized polyurethane film for site-specific gene therapy].
OBJECTIVE
To study the feasibility of delivering viral gene vector from a collagen-coated polyurethane (PU) film through a mechanism involving monoclonal antiviral antibody tethering.


METHODS
Anti-adenoviral monoclonal antibodies were covalently bound to the collagen-coated PU surface. These antibodies enabled tethering of replication defective adenoviruses through highly specific antigen-antibody affinity. The PU film-based gene delivery using antibody-tethered adenovirus encoding green fluorescent protein (GEP) was tested in rat arterial smooth muscle cell (A10 cell) culture in vitro. The virus binding stability was studied by incubating the collagen-coated PU film in PBS solution at 37 degrees C for 20 days, followed with A10 cell cultures with the incubated films and the corresponding buffer solution.


RESULTS
PU films with antibody-tethered adenovirus encoding GFP demonstrated efficient and highly localized gene delivery to A10 cells. Virus binding was stable for at least 10 days at physiological conditions, more than 77% of the originally bound virus remained in the film after 15 day's incubation.


CONCLUSIONS
Gene delivery using PU film-based anti-viral antibody tethering of vectors exhibited potentials of applications in a wide array of single or multiple therapeutic gene strategies, and in further stent-based gene delivery therapeutic strategies.
Zhang
Lin-Hua
LH
Tianjin Key Laboratory of Biomaterial Research, Laboratory of Drug Controlled Release, Institute of Biomedical Engineering, CAMS and PUMC, Tianjin 300192, China.
Song
Cun-Xian
CX
Wang
Man-Yan
MY
Yang
Jing
J
Tang
Li-Na
LN
D000256:Adenoviridae / Q000235:genetics; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D011140:Polyurethanes / Q000737:chemistry*; D011485:Protein Binding
D000911:Antibodies, Monoclonal; D011140:Polyurethanes