title: Effect of postharvest storage on the expression of the apple allergen Mal d 1.
abstract: Consumption of fresh apples can cause allergy in susceptible individuals. A competitive enzyme-linked immunosorbent assay (ELISA) has been developed to determine Mal d 1 levels in apple pulp using a monoclonal antibody (BIP-1). The ELISA was able to rank ten cultivars according to their Mal d 1 content (between 3.8 and 72.5 mug/g pulp). For the first time, it has been demonstrated that growing conditions and postharvest storage, using three different treatments over a 5 month period in 2 consecutive years, increase Mal d 1 expression at a translational and transcriptional level (3.5- and 8.5-fold under controlled atmosphere storage). Expression of three major Mal d 1 isoforms was observed by real-time polymerase chain reaction over the 5 month storage period, and Mal d 1.02 was the most highly expressed isoform. In conclusion, Mal d 1 gene expression was significantly increased during modified atmosphere storage. Individuals suffering from birch pollen-apple allergy syndrome might experience fewer problems consuming freshly picked apples.
authors.affiliation: Institute of Food Research, Norwich Research Park, Colney, UK. ana.sancho@bbsrc.ac.uk
authors.forename: Ana I
authors.initials: AI
authors.lastname: Sancho
authors.forename: Robert
authors.initials: R
authors.lastname: Foxall
authors.forename: Tom
authors.initials: T
authors.lastname: Browne
authors.forename: Rickmer
authors.initials: R
authors.lastname: Dey
authors.forename: Laurian
authors.initials: L
authors.lastname: Zuidmeer
authors.forename: Gorji
authors.initials: G
authors.lastname: Marzban
authors.forename: Keith W
authors.initials: KW
authors.lastname: Waldron
authors.forename: Ronald
authors.initials: R
authors.lastname: van Ree
authors.forename: Karin
authors.initials: K
authors.lastname: Hoffmann-Sommergruber
authors.forename: Margit
authors.initials: M
authors.lastname: Laimer
authors.forename: E N Clare
authors.initials: EN
authors.lastname: Mills
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000235:genetics; D052179:Antigens, Plant; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005519:Food Preservation / Q000379:methods*; D005638:Fruit / Q000737:chemistry / Q000276:immunology*; D015870:Gene Expression; D027845:Malus; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C095551:MALD1 protein, Malus domestica; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant