title: Formation of GW bodies is a consequence of microRNA genesis.
abstract: GW bodies (GWBs), or mammalian P bodies, proposed to be involved in messenger RNA storage and/or degradation, have recently been linked to RNA interference and microRNA (miRNA) processing. We report that endogenous let-7 miRNA co-precipitates with the GW182 protein complex. In addition, knockdown of two proteins, Drosha and its protein partner DGCR8, which are vital to the generation of mature miRNA, results in the loss of GWBs. Subsequent introduction of short interference RNA specific to lamin A/C is accompanied by reassembly of GWBs and concurrent knockdown of lamin A/C protein. Taken together, these studies show that miRNAs are crucial components in GWB formation.
authors.affiliation: Department of Oral Biology, University of Florida, 1600 SW Archer Road, PO Box 100424, Gainesville, 32610-0424, USA.
authors.forename: Kaleb M
authors.initials: KM
authors.lastname: Pauley
authors.forename: Theophany
authors.initials: T
authors.lastname: Eystathioy
authors.forename: Andrew
authors.initials: A
authors.lastname: Jakymiw
authors.forename: John C
authors.initials: JC
authors.lastname: Hamel
authors.forename: Marvin J
authors.initials: MJ
authors.lastname: Fritzler
authors.forename: Edward K L
authors.initials: EK
authors.lastname: Chan
mesh_terms: D022083:Cytoplasmic Structures / Q000378:metabolism* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism* / Q000502:physiology; D008954:Models, Biological; D020871:RNA Stability; D034443:RNA Transport; D043244:Ribonuclease III / Q000235:genetics*; D014162:Transfection
chemical_list: D035683:MicroRNAs; C517918:DROSHA protein, human; D043244:Ribonuclease III