Genetic engineering of embryonic stem cells.
We describe a novel generation of lentiviral vectors that are particularly well suited for work with embryonic stem cells. The possibility of selecting cell lines with antibiotics and the rapid insertion of any combination of promoters and genes of interest makes them a powerful tool in the generation of transgenic ES cell lines. This vector can also greatly facilitate studies aimed at the improvement of neuronal engineering from ES cells, by making it possible to monitor the emergence and differentiation of neurons.
Suter
David M
DM
Department of Pathology and Immunology, Geneva Medical School, Switzerland.
Dubois-Dauphin
Michel
M
Krause
Karl-Heinz
KH
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D004622:Embryo, Mammalian / Q000166:cytology*; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics; D006678:HIV / Q000235:genetics; D006801:Humans; D009474:Neurons / Q000637:transplantation; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology