title: Rhythmic clock gene expression in heart, kidney and some brain nuclei involved in blood pressure control in hypertensive TGR(mREN-2)27 rats.
abstract: Hypertensive TGR(mREN-2)27 rats exerting inverted blood pressure (BP) profile were used to study clock gene expression in structures responsible for BP control. TGR and control Sprague Dawley male rats were synchronized to the light:dark cycle 12:12 with food and water ad libitum. Daily rhythm in per2, bmal1, clock and dbp expression in the suprachiasmatic nucleus (SCN), rostral ventrolateral medulla (RVLM), nucleus of the solitary tract (NTS), heart and kidney was determined in both groups. Sampling occurred in regular 4 h intervals when rats of both strains were 11-weeks-old. Blood pressure and relative heart weight were significantly elevated in TGR rats in comparison with control. Expression of bmal1 and clock was up regulated in SCN of TGR rats but daily rhythm in per2 and dbp expression was similar in both groups. Mesor of per2 expression in RVLM was significantly higher in TGR than in control rats. In NTS of TGR rats expression of per2 was phase delayed by 3.5 h in comparison with control and bmal1 did not exert rhythmic pattern. Our study provided the first evidence about modified function of central and peripheral circadian oscillators in TGR rats at the level of clock gene expression. Expression of clock genes exerted up regulation in SCN and RVLM and down regulation in NTS. Circadian oscillators in selected brain structures were influenced more than oscillators in the heart and kidney by additional renin gene. Interactions of RAS and circadian system probably contribute to the development of inverted BP profile in TGR rats.
authors.affiliation: Department of Animal Physiology and Ethology, Comenius University Bratislava, Mlynská dolina B2, 842 15, Bratislava, Slovak Republic.
authors.forename: Iveta
authors.initials: I
authors.lastname: Herichová
authors.forename: Boris
authors.initials: B
authors.lastname: Mravec
authors.forename: Katarína
authors.initials: K
authors.lastname: Stebelová
authors.forename: Ol'ga
authors.initials: O
authors.lastname: Krizanová
authors.forename: Dana
authors.initials: D
authors.lastname: Jurkovicová
authors.forename: Richard
authors.initials: R
authors.lastname: Kvetnanský
authors.forename: Michal
authors.initials: M
authors.lastname: Zeman
mesh_terms: D056930:ARNTL Transcription Factors; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D001683:Biological Clocks / Q000235:genetics*; D001794:Blood Pressure / Q000502:physiology*; D056926:CLOCK Proteins; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D006321:Heart / Q000502:physiology*; D007668:Kidney / Q000502:physiology*; D008297:Male; D008526:Medulla Oblongata / Q000033:anatomy & histology / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D056950:Period Circadian Proteins; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D017207:Rats, Sprague-Dawley; D012084:Renin-Angiotensin System / Q000502:physiology; D017552:Solitary Nucleus / Q000166:cytology / Q000502:physiology; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D056930:ARNTL Transcription Factors; D051792:Basic Helix-Loop-Helix Proteins; D056926:CLOCK Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D056950:Period Circadian Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C485975:DBP protein, rat; C513311:Per2 protein, rat; C538945:Clock protein, rat