title: Glycomic studies of Drosophila melanogaster embryos.
abstract: With the complete genome sequence of Drosophila melanogaster defined a systematic approach towards understanding the function of glycosylation has become possible. Structural assignment of the entire Drosophila glycome during specific developmental stages could provide information that would shed further light on the specific roles of different glycans during development and pinpoint the activity of certain glycosyltransferases and other glycan biosynthetic genes that otherwise might be missed through genetic analyses. In this paper the major glycoprotein N- and O-glycans of Drosophila embryos are described as part of our initial undertaking to characterize the glycome of Drosophila melanogaster. The N-glycans are dominated by high mannose and paucimannose structures. Minor amounts of mono-, bi- and tri-antennary complex glycans were observed with GlcNAc and Galbeta1-4GlcNAc non-reducing end termini. O-glycans were restricted to the mucin-type core 1 Galbeta1-3GalNAc sequence.
authors.affiliation: Division of Molecular Biosciences, Faculty of Life Sciences, Imperial College, London, SW7 2AZ, UK.
authors.forename: Simon J
authors.initials: SJ
authors.lastname: North
authors.forename: Kate
authors.initials: K
authors.lastname: Koles
authors.forename: Caleb
authors.initials: C
authors.lastname: Hembd
authors.forename: Howard R
authors.initials: HR
authors.lastname: Morris
authors.forename: Anne
authors.initials: A
authors.lastname: Dell
authors.forename: Vladislav M
authors.initials: VM
authors.lastname: Panin
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Haslam
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides