Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains.
Bezerra
Claudia C F
CC
Serviço de Micologia, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Av. Brazil 4365, 21040-900 Rio de Janeiro, RJ, Brazil.
de Lima
Renata F
RF
Lazera
Márcia S
MS
Wanke
Bodo
B
Borba
Cíntia M
CM
D001938:Brazil; D003045:Coccidioides / Q000145:classification / Q000235:genetics*; D050296:Microbial Viability; D016133:Polymerase Chain Reaction; D011309:Preservation, Biological / Q000379:methods*; D013997:Time Factors