title: Laminin-332 alters connexin profile, dye coupling and intercellular Ca2+ waves in ciliated tracheal epithelial cells.
abstract: BACKGROUND
  Tracheal epithelial cells are anchored to a dynamic basement membrane that contains a variety of extracellular matrix proteins including collagens and laminins. During development, wound repair and disease of the airway epithelium, significant changes in extracellular matrix proteins may directly affect cell migration, differentiation and events mediated by intercellular communication. We hypothesized that alterations in cell matrix, specifically type I collagen and laminin alpha3beta3gamma2 (LM-332) proteins within the matrix, directly affect intercellular communication in ciliated rabbit tracheal epithelial cells (RTEC).
  
  
  METHODS
  Functional coupling of RTEC was monitored by microinjection of the negatively charged fluorescent dyes, Lucifer Yellow and Alexa 350, into ciliated RTEC grown on either a LM-332/collagen or collagen matrix. Coupling of physiologically significant molecules was evaluated by the mechanism and extent of propagated intercellular Ca2+ waves. Expression of connexin (Cx) mRNA and proteins were assayed by reverse transcriptase - polymerase chain reaction and immunocytochemistry, respectively.
  
  
  RESULTS
  When compared to RTEC grown on collagen alone, RTEC grown on LM-332/collagen displayed a significant increase in dye transfer. Although mechanical stimulation of RTEC grown on either LM-332/collagen or collagen alone resulted in intercellular Ca2+ waves, the mechanism of transfer was dependent on matrix: RTEC grown on LM-332/collagen propagated Ca2+waves via extracellular purinergic signaling whereas RTEC grown on collagen used gap junctions. Comparison of RTEC grown on collagen or LM-332/collagen matrices revealed a reorganization of Cx26, Cx43 and Cx46 proteins.
  
  
  CONCLUSIONS
  Alterations in airway basement membrane proteins such as LM-332 can induce connexin reorganizations and result in altered cellular communication mechanisms that could contribute to airway tissue function.
authors.affiliation: Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, University of Virginia Charlottesville, Virginia 22908, USA. bei6n@virginia.edu
authors.forename: Brant E
authors.initials: BE
authors.lastname: Isakson
authors.forename: Colin E
authors.initials: CE
authors.lastname: Olsen
authors.forename: Scott
authors.initials: S
authors.lastname: Boitano
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000166:cytology / Q000502:physiology; D020013:Calcium Signaling / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D002923:Cilia / Q000378:metabolism; D017630:Connexins / Q000096:biosynthesis* / Q000378:metabolism; D017629:Gap Junctions / Q000378:metabolism; D007797:Laminin / Q000008:administration & dosage / Q000502:physiology*; D011817:Rabbits; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*; D014132:Trachea / Q000166:cytology* / Q000378:metabolism
chemical_list: D017630:Connexins; D007797:Laminin; C097043:laminin alpha5