[Effect of combination of glutamine and mitomycin C on human gastric carcinoma cell strain MGC-803].
OBJECTIVE
To evaluate the effect of combination of glutamine (GLN) and mitomycin C (MMC) on the human gastric carcinoma cell line MGC-803 in vitro.


METHODS
The effects of GLN and MMC were measured by MTT assay, and the interaction between the two agents was evaluated by the median-effect principle. Flow cytometry was used for cell cycle analysis.


RESULTS
GLN did not significantly stimulate the cell growth in vitro. High-concentration of GLN could inhibit the cell growth. MMC could effectively inhibit the cell growth in a time-dependent manner. The interaction of these two agents showed a weak antagonistic activity (1 < CI < 1.2703). MMC induced remarkable S-phase arrest. Low-dose GLN has limited effect on the S-phase arrest of MMC, while high-dose GLN significantly attenuated the S-phase arrest and lowered the proliferation index of MGC-803 cell.


CONCLUSIONS
Combination of GLN and MMC has a a weak and dose-dependent antagonistic activity in the treatment of gastric carcinoma cell line MGC-803. The combination of high-dose MMC and low-dose GLN may achieve better efficacy.
Peng
Wei-Zhao
WZ
Department of General Surgery, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Yu
Jian-Chun
JC
Liu
Yu-Qin
YQ
Kang
Wei-Ming
WM
Fan
Yue-Ping
YP
Guo
Wei-Dong
WD
Zhang
Qun
Q
D000230:Adenocarcinoma / Q000473:pathology; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005973:Glutamine / Q000494:pharmacology*; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000473:pathology*
D000903:Antibiotics, Antineoplastic; D005973:Glutamine; D016685:Mitomycin