Altered peptide ligands control type II collagen-reactive T cells from rheumatoid arthritis patients.
We previously reported that peripheral blood mononuclear cells from HLA-DRB1*0101 Japanese patients with rheumatoid arthritis (RA) were highly reactive to 256-271 peptide of type II collagen (CII). In this report, we tried to regulate the CII reactivity of T cells from RA patients with HLA-DRB1*0101 by altered peptide ligand (APL), which is a single amino acid substitution of the T-cell epitope on CII 256-271 peptide. Antagonistic activity of 21 APLs was assessed using three different T-cell lines. Results showed that 262 (G-->A) APL of CII 256-271 exhibited antagonistic activity in all T-cell lines and it was suggested that the application of CII APL might be a new therapeutic strategy in the regulation of RA.
Ohnishi
Yasuyuki
Y
Division of Rheumatology, Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tenodai, Tsukuba, 305-8575, Japan.
Tsutsumi
Akito
A
Matsumoto
Isao
I
Goto
Daisuke
D
Ito
Satoshi
S
Kuwana
Masataka
M
Uemura
Yasushi
Y
Nishimura
Yasuharu
Y
Sumida
Takayuki
T
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000378:metabolism / Q000628:therapy*; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D015234:HLA-A Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D008213:Lymphocyte Activation / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D011948:Receptors, Antigen, T-Cell / Q000037:antagonists & inhibitors / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D024043:Collagen Type II; D015234:HLA-A Antigens; D059811:HLA-DRB1 Chains; C487961:HLA-DRB1*01:01 antigen; D008024:Ligands; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell