Dodeca-CLE peptides as suppressors of plant stem cell differentiation.
In plants and animals, small peptide ligands that signal in cell-cell communication have been suggested to be a crucial component of development. A bioassay of single-cell transdifferentation demonstrates that a dodecapeptide with two hydroxyproline residues is the functional product of genes from the CLE family, which includes CLAVATA3 in Arabidopsis. The dodecapeptide suppresses xylem cell development at a concentration of 10(-11) M and promotes cell division. An application, corresponding to all 26 Arabidopsis CLE protein family members, of synthetic dodecapeptides reveals two counteracting signaling pathways involved in stem cell fate.
Ito
Yasuko
Y
Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Nakanomyo
Ikuko
I
Motose
Hiroyasu
H
Iwamoto
Kuninori
K
Sawa
Shinichiro
S
Dohmae
Naoshi
N
Fukuda
Hiroo
H
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism; D019659:Asteraceae / Q000166:cytology*; D001483:Base Sequence; D001681:Biological Assay; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D008024:Ligands; D018519:Meristem / Q000166:cytology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development; D018514:Plant Structures / Q000166:cytology*; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology*
C118167:AT2G27250 protein, Arabidopsis; D029681:Arabidopsis Proteins; D008024:Ligands; D009842:Oligopeptides; D010940:Plant Proteins