[Study on structures of blattela germanica allergen, Bla g 2 expressed by eukaryotic and prokaryotic vectors using fluorescence and CD spectra].
The recombinant allergen, Bla g 2, was expressed by prokaryotic vector E. coli and eukaryotic vector P. Pastoris. The different structures and configurations of the Bla g 2 from E. coli and P. Pastoris were studied by fluorescence and circular dichroism. The secondary structures of Bla g 2 in solution, and the composition besides the type of its tertiary structure were proposed. These studies help understand the differences between prokaryotic and eukaryotic expression systems, reveal the relationship between the structure and the function of Bla g 2, and improve the production of this significant allergen.
Liu
Zhi-Gang
ZG
College of Life Science, Shenzhen University, Shenzhen 518060, China.
Zhu
Jian-Qi
JQ
Huang
Hai-Zhen
HZ
Xu
Hong
H
D000485:Allergens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D002942:Circular Dichroism / Q000379:methods*; D003058:Cockroaches / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary
D000485:Allergens; D019476:Insect Proteins; D016282:Aspartic Acid Endopeptidases; C094699:allergen Bla g 2