[Determination of chloramphenicol residues in animal tissues by LC-MS/MS method].
A liquid chromatographic method with mass spectrometric determination and identification (LC-MS/MS) for the determination of chloramphenicol (CAP) in tissues of animal origin was presented. Homogenized samples were extracted with ethyl acetate and evaporated to dryness followed by a clean-up using the liquid-liquid distribution between acetonitryle and hexane. Chloramphenicol was determined by LC-MS-ESI-MS in negative mode. There was used Phenomenex column with the mixture of acetonitrile-water as a mobile phase. The method was validated according to the criteria of Decision Commission No 2002/657/EC. Samples were fortified at CAP levels between 0.1 and 0.45 ng/g with 5d-CAP as internal standard. Recoveries for the level 0.3 ng/g were in the range 76.3-100.3%. Limit of decision (CCalpha) was between 0.137-0.205 ng/g. Limit of quantification (LOQ)--0.1 ng/g.
Rodziewicz
Lech
L
Pracownia Badań Chemicznych Srodków Spozywczych, Zakład Higieny Weterynaryjnej, Białystok. rodziewicz@wiw.bianet.com.pl
Zawadzka
Iwona
I
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002701:Chloramphenicol / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D000900:Anti-Bacterial Agents; D002701:Chloramphenicol