Pin1 gene mutation is a rare event in gastric cancer.
The peptidyl-prolyl isomerase Pin1 is strikingly overexpressed in human cancers and is a novel regulator of beta-catenin. To determine whether somatic mutation of the Pin1 gene is involved in the development and/or progression of gastric cancer, we searched for mutations of the Pin1 gene in 95 gastric cancer specimens. The effect of Pin1 on beta-catenin expression was further examined in wild- and mutant-type Pin1-transfected HEK 293T cells. We found only one missense mutation that led to the substitution of alanine by aspartic acid at codon 118 of the Pin1 gene. On transfection study, the mutant Pin1 showed an increased expression of beta-catenin. However, the mutation had no effect on expression of the Pin1 protein in the case with Pin1 mutation. These results suggest that Pin1 may not play a role in the development or progression of gastric cancer.
Kim
Chang Jae
CJ
Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Song
Jae Hwi
JH
Cho
Yong Gu
YG
Chae
Hiun Suk
HS
Nam
Suk Woo
SW
Yoo
Nam Jin
NJ
Lee
Jung Young
JY
Park
Won Sang
WS
D002460:Cell Line; D004252:DNA Mutational Analysis; D018450:Disease Progression; D006801:Humans; D016297:Mutagenesis, Site-Directed; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D019696:Peptidylprolyl Isomerase / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D018807:Polymorphism, Single-Stranded Conformational; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D000072340:NIMA-Interacting Peptidylprolyl Isomerase; C000606846:PIN1 protein, human; D019696:Peptidylprolyl Isomerase