title: Regulation of sexual development of Plasmodium by translational repression.
abstract: Translational repression of messenger RNAs (mRNAs) plays an important role in sexual differentiation and gametogenesis in multicellular eukaryotes. Translational repression and mRNA turnover were shown to influence stage-specific gene expression in the protozoan Plasmodium. The DDX6-class RNA helicase, DOZI (development of zygote inhibited), is found in a complex with mRNA species in cytoplasmic bodies of female, blood-stage gametocytes. These translationally repressed complexes are normally stored for translation after fertilization. Genetic disruption of pbdozi inhibits the formation of the ribonucleoprotein complexes, and instead, at least 370 transcripts are diverted to a degradation pathway.
authors.affiliation: Department of Parasitology, Leiden University Medical Centre, 2333 ZA Leiden, Netherlands.
authors.forename: Gunnar R
authors.initials: GR
authors.lastname: Mair
authors.forename: Joanna A M
authors.initials: JA
authors.lastname: Braks
authors.forename: Lindsey S
authors.initials: LS
authors.lastname: Garver
authors.forename: Joop C A G
authors.initials: JC
authors.lastname: Wiegant
authors.forename: Neil
authors.initials: N
authors.lastname: Hall
authors.forename: Roeland W
authors.initials: RW
authors.lastname: Dirks
authors.forename: Shahid M
authors.initials: SM
authors.lastname: Khan
authors.forename: George
authors.initials: G
authors.lastname: Dimopoulos
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Janse
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Waters
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D017125:Genes, Protozoan; D020411:Oligonucleotide Array Sequence Analysis; D010961:Plasmodium / Q000201:enzymology / Q000235:genetics; D010962:Plasmodium berghei / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D014176:Protein Biosynthesis; D015800:Protozoan Proteins / Q000378:metabolism; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D023121:RNA, Messenger, Stored / Q000378:metabolism*; D016053:RNA, Protozoan / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism
chemical_list: D015800:Protozoan Proteins; D012333:RNA, Messenger; D023121:RNA, Messenger, Stored; D016053:RNA, Protozoan; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; D020365:RNA Helicases