High-sensitivity detection for model organophosphorus and carbamate pesticide with quartz crystal microbalance-precipitation sensor.
The effect of acetylcholinesterase (AChE) immobilization over the surface of a quartz crystal microbalance (QCM), by chemisorption of the AChE thiolated with a heterobifunctional cross-linker, sulfo-succinimidyl-6-[3-(2-pyridyldithio)propionamido]hexanoate, and carboxyl-amine coupling of AChE to 3-mercaptopropionic acid self-assembled monolayer, on the responses of a batch-type QCM-precipitation sensor was compared, resulting in a better sensitivity and binding efficiency in the former method. When an inhibition study with the developed sensor was undertaken at the optimized AChE immobilization with varying concentrations of a model organophosphorus pesticide EPN and carbamate one carbofuran, a sensitive detection for them was possible with the limit of detection corresponding to 1.55 x 10(-8) and 1.30 x 10(-9)M, respectively.
Kim
Namsoo
N
Korea Food Research Institute, San 46-1, Baekhyun-dong, Bundang-gu, Songnam-si, Kyonggi-do 463-746, Republic of Korea. k9130sen@hanmail.net
Park
In-Seon
IS
Kim
Dong-Kyung
DK
D015374:Biosensing Techniques / Q000379:methods*; D002235:Carbofuran / Q000032:analysis*; D011232:Chemical Precipitation; D004800:Enzymes, Immobilized; D007306:Insecticides / Q000032:analysis*; D009943:Organophosphorus Compounds / Q000032:analysis*; D011791:Quartz; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D007306:Insecticides; D009943:Organophosphorus Compounds; D011791:Quartz; D002235:Carbofuran