Centrally administered neuropeptide W-30 activates magnocellular neurosecretory cells in the supraoptic and paraventricular nuclei with neurosecretion in rats.
We examined the effects of i.c.v. administration of neuro-peptide W-30 (NPW30) on plasma arginine vasopressin (AVP) and plasma oxytocin (OXT) using RIA. The induction of c-fos mRNA, AVP heteronuclear (hn)RNA, and c-Fos protein (Fos) in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of rats were also investigated using in situ hybridization histochemistry for c-fos mRNA and AVP hnRNA, and immunohistochemistry for Fos. Both plasma AVP and OXT were significantly increased at 5 and 15 min after i.c.v. administration of NPW30 (2.8 nmol/rat). In situ hybridization histochemistry revealed that the induction of c-fos mRNA and AVP hnRNA in the SON and PVN were significantly increased 15, 30, and 60 min after i.c.v. administration of NPW30 (1.4 nmol/rat). Dual immunostaining for Fos/AVP and Fos/OXT revealed that both AVP-like immunoreactive (LI) cells and OXT-LI cells exhibited nuclear Fos-LI in the SON and PVN, 90 min after i.c.v. administration of NPW30 (2.8 nmol/rat). These results suggest that central NPW30 may be involved in the regulation of secretion of AVP and OXT in the magnocellular neurosecretory cells in the SON and PVN.
Kawasaki
Makoto
M
Departments of Physiology, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
Onaka
Tatsushi
T
Nakazato
Masamitsu
M
Saito
Jun
J
Mera
Takashi
T
Hashimoto
Hirofumi
H
Fujihara
Hiroaki
H
Okimoto
Nobukazu
N
Ohnishi
Hideo
H
Nakamura
Toshitaka
T
Ueta
Yoichi
Y
D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis / Q000097:blood* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos; D017403:In Situ Hybridization / Q000379:methods; D007276:Injections, Intraventricular; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology*; D010121:Oxytocin / Q000032:analysis / Q000097:blood*; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling; D013495:Supraoptic Nucleus / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
C467731:NPW protein, human; D009479:Neuropeptides; D012333:RNA, Messenger; D001127:Arginine Vasopressin; D010121:Oxytocin