title: Quantitation of mercapturic acids from acrylamide and glycidamide in human urine using a column switching tool with two trap columns and electrospray tandem mass spectrometry.
abstract: A sensitive and specific electrospray tandem mass spectrometry method using a column switching unit with two trap columns was established to quantify the mercapturates (MAs) of acrylamide (AA) and glycidamide (GA) in human urine. A specially endcapped material was applied for trapping the hydrophilic MAs and a pre-trap column was used to remove lipophilic compounds from the directly injected urine to protect the trap column. The limits of quantitation for AA-MA and GA-MA in urine were 0.5 microg/L and 1 microg/L, respectively. Urine was spiked with deuterated internal standards and injected directly into LC-MS/MS. Urine of smokers (n=13) revealed the highest concentrations of AA-MA and GA-MA in the range of 61-706 microg/L and 5-54 microg/L, respectively. Lower levels for AA-MA (14-102 microg/L) and GA-MA (1-11 microg/L) were detected in non-smokers (n=13).
authors.affiliation: Department of Toxicology, University of Würzburg, Versbacher Strasse 9, 97078 Würzburg, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Kellert
authors.forename: Karoline
authors.initials: K
authors.lastname: Scholz
authors.forename: Silvia
authors.initials: S
authors.lastname: Wagner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Dekant
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Völkel
mesh_terms: D000111:Acetylcysteine / Q000032:analysis*; D020106:Acrylamide / Q000737:chemistry / Q000652:urine*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004852:Epoxy Compounds / Q000737:chemistry / Q000652:urine*; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D004852:Epoxy Compounds; D020106:Acrylamide; C071834:glycidamide; D000111:Acetylcysteine