title: Enantiomeric resolution of bifonazole by supercritical fluid chromatography.
abstract: The enantiomeric separation of bifonazole by supercritical fluid chromatography on Chiralpak AD has been studied. The effect of different modifiers (methanol, ethanol, 2-propanol, and acetonitrile) was examined. Enantioseparation was possible with all of them, but the best results were provided by the alcohol-type ones. The resolution was higher than 5 in all cases. The isoelution temperatures Tiso were calculated from the study of the temperature effect for the different organic modifiers. The value of Tiso was below the working temperature range on using methanol, but above it with ethanol or 2-propanol.
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, University of Valladolid, Valladolid, Spain. ltoribio@qa.uva.es
authors.forename: Laura
authors.initials: L
authors.lastname: Toribio
authors.forename: Ma Jesús
authors.initials: MJ
authors.lastname: del Nozal
authors.forename: José L
authors.initials: JL
authors.lastname: Bernal
authors.forename: Cristina
authors.initials: C
authors.lastname: Alonso
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Jiménez
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry*; D015394:Molecular Structure; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D007093:Imidazoles; C036596:bifonazole