title: Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis.
abstract: A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients with culture-confirmed melioidosis. All six patients with melioidosis septic shock were blood PCR positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy.
authors.affiliation: Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.
authors.forename: Ella M
authors.initials: EM
authors.lastname: Meumann
authors.forename: Ryan T
authors.initials: RT
authors.lastname: Novak
authors.forename: Daniel
authors.initials: D
authors.lastname: Gal
authors.forename: Mirjam E
authors.initials: ME
authors.lastname: Kaestli
authors.forename: Mark
authors.initials: M
authors.lastname: Mayo
authors.forename: Joshua P
authors.initials: JP
authors.lastname: Hanson
authors.forename: Emma
authors.initials: E
authors.lastname: Spencer
authors.forename: Mindy B
authors.initials: MB
authors.lastname: Glass
authors.forename: Jay E
authors.initials: JE
authors.lastname: Gee
authors.forename: Patricia P
authors.initials: PP
authors.lastname: Wilkins
authors.forename: Bart J
authors.initials: BJ
authors.lastname: Currie
mesh_terms: D001692:Biological Transport / Q000235:genetics; D001769:Blood / Q000382:microbiology; D001826:Body Fluids / Q000382:microbiology; D016957:Burkholderia pseudomallei / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D019353:Endemic Diseases; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial