title: Proteomic analysis of the autoantibody response following immunization with a single autoantigen.
abstract: In most autoimmune diseases, the autoantibody response is directed against several antigens of the target organ whose identification is crucial for understanding the physiopathological process. Thus, technologies allowing a characterization of the whole autoantibody pattern of both human and experimental autoimmune diseases are required. Here we have used immunoproteomic analysis of human epidermal extracts to characterize the diversity of the anti-desmosome antibody response induced in normal mice immunized with desmoglein 1, the major autoantigen of pemphigus foliaceus, an autoimmune blistering skin disease. In particular, this analysis enables us to characterize the binding properties of anti-desmosome mAbs derived from these mice and to show that the autoantibody response induced upon immunization with a single autoantigen targets different epidermal autoantigens with a pattern similar to that observed in certain variety of human pemphigus.
authors.affiliation: Inserm, U519 and IFR23, Faculté de Médecine et de Pharmacie, Rouen, France.
authors.forename: Hugo
authors.initials: H
authors.lastname: Mouquet
authors.forename: Laurent
authors.initials: L
authors.lastname: Drouot
authors.forename: Roland
authors.initials: R
authors.lastname: Charlionnet
authors.forename: Christophe
authors.initials: C
authors.lastname: Arnoult
authors.forename: Florence
authors.initials: F
authors.lastname: Bonnet-Bayeux
authors.forename: Marlène
authors.initials: M
authors.lastname: Thomas
authors.forename: Jérôme
authors.initials: J
authors.lastname: Leprince
authors.forename: Pascal
authors.initials: P
authors.lastname: Joly
authors.forename: François
authors.initials: F
authors.lastname: Tron
authors.forename: Danièle
authors.initials: D
authors.lastname: Gilbert
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D051183:Desmoglein 1 / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D051179:Plakins / Q000276:immunology; D011498:Protein Precursors / Q000737:chemistry / Q000276:immunology; D040901:Proteomics; D012867:Skin / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001324:Autoantigens; D051183:Desmoglein 1; D008565:Membrane Proteins; C491674:PPL protein, human; D051179:Plakins; D011498:Protein Precursors; C100754:envoplakin