title: Ameliorative effects of vitamin supplementation on ethyl methane sulphonate-induced genotoxicity in a fish, Anabas testudineus.
abstract: The efficacy of 0.02% vitamin C (VC; l-ascorbic acid) and 0.05% beta-carotene (BC) at the rate of 1 ml/100g of body weight in amelioration of ethyl methane sulphonate (EMS)-induced genotoxicity has been studied in an Indian endemic fish, Anabas testudineus by using several cytogenetical endpoints like chromosome aberrations, micronuclei (MN) and abnormal nuclei (AN), and sperm head anomaly at 6, 24, 48, 72 and 96 h after treatment, as compared to suitable controls (distilled water (DW)-treated control for EMS and VC-treated fish, and 1% alcohol-treated control for BC-treated fish). Both VC and BC reduced EMS-induced genotoxicity at all the fixation intervals as compared to their respective controls. Additionally, effects of two more doses of VC (0.01% and 0.05%) and BC (0.02% and 0.1%) were analyzed at 72 h after treatment (at the peak period of EMS genotoxicity) for testing their relative efficacy in amelioration of EMS-induced cytogenetical damage in this fish. All the three doses of both VC and BC appeared to reduce the EMS-induced genotoxicity in this fish to a variable extent, of which the higher dose of VC appeared to give marginally better protection while the dose-response relationship was inconclusive for BC. The results of this study can lead to future research for exploring if low doses of these vitamins may be useful in protecting fish from genotoxic damage on exposure to mutagenic agents in small confined/stagnant waters.
authors.affiliation: Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani-741235, West Bengal, India.
authors.forename: Bibhas
authors.initials: B
authors.lastname: Guha
authors.forename: Jayanta Kumar
authors.initials: JK
authors.lastname: Das
authors.forename: Anisur Rahman
authors.initials: AR
authors.lastname: Khuda-Bukhsh
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000008:administration & dosage*; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000187:drug effects; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004900:Erythroblasts / Q000187:drug effects / Q000473:pathology; D004913:Erythrocytes, Abnormal / Q000187:drug effects / Q000473:pathology; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced / Q000187:drug effects; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D010471:Perches / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D019207:beta Carotene / Q000008:administration & dosage*
chemical_list: D016587:Antimutagenic Agents; D009153:Mutagens; D019207:beta Carotene; D005020:Ethyl Methanesulfonate; D001205:Ascorbic Acid