A retrovirus-based system to stably silence hepatitis B virus genes by RNA interference.
RNA interference (RNAi) might be an efficient antiviral therapy for some obstinate illness. Herein, a retrovirus-based RNAi system was developed to drive expression and delivery of Hepatitis B virus (HBV)-specific short hairpin RNA (shRNA) in HepG2 cells. The levels of HBsAg and HBeAg and that of HBV mRNA were dramatically decreased by this RNAi system in HepG2 cells transfected with Topo-HBV plasmid. Retrovirus-based RNAi thus may be useful for therapy in HBV and other viral infections and provide new clues for prophylactic vaccine development.
Jia
Fang
F
School of Life Sciences, Sichuan University, Chengdu , 610064, China.
Zhang
Yi-Zheng
YZ
Liu
Chang-Mei
CM
D045744:Cell Line, Tumor; D005814:Genes, Viral; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis; D006513:Hepatitis B e Antigens / Q000096:biosynthesis; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D034622:RNA Interference; D012190:Retroviridae / Q000235:genetics*
D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens