Small bowel and colon transplantation in rats using porto-portal cuff anastomosis.
Portal versus systemic venous drainage and colon grafting are major controversies in the techniques of intestinal transplantation. The rat is the best animal for research in this field. Nevertheless, this model requires complex microvascular anastomoses that are responsible for the high incidence of technical failures. A cuff technique is an easier anastomosis method than a hand-suture. We describe a simplified rat model of small bowel and colon transplantation using a porto-portal cuff anastomosis. DONOR: The entire small bowel, cecum, and ascending colon are harvested on a vascular pedicle, consisting of a long aortomesenteric conduit and portal vein. The right colonic vessels are preserved. The graft is flushed and a cuff device is placed on the end of the portal vein. RECIPIENT: The graft is implanted through an end-to-side aorto-aorta hand-sewn anastomosis. A segment between the first and second jejunal branch is isolated between clamps to insert into the portal cuff. After reperfusion, the recipient's mesentery is divided just below the cuff anastomosis. The recipient jejunum, ileum, and ascending colon are removed en bloc, and the graft is anastomosed in continuity with the remaining naive intestine concluding the operation. This simplified technique surmounts the technical obstacles in rats because it is easily and quickly performed, maintaining the physiological portal drainage, preserving graft ileocecal valve and ascending colon, and reaching acceptable success after a short period of training.
Galvão
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FH
Transplantation and Liver Surgery Discipline, University of Sao Paulo, Av. Dr. Arnaldo 455, Sao Paulo-SP, 01246-903 Brazil. fgalvao@usp.br
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Neto
A Bakonyi
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Machado
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Bacchella
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D000714:Anastomosis, Surgical; D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D003106:Colon / Q000098:blood supply / Q000637:transplantation*; D007082:Ileum / Q000637:transplantation; D007421:Intestine, Small / Q000098:blood supply / Q000637:transplantation*; D023421:Models, Animal; D011168:Portal System; D051381:Rats