title: Determination of proteins in infant formula by high-performance liquid chromatography-electrospray tandem mass spectrometry.
abstract: To determine the protein content of formula, gel electrophoresis was performed on the infant formula samples and the entire protein patterns were analyzed by nano-high performance liquid chromatography-electrospray tandem mass spectrometry (nano-HPLC/ESI/MS/MS). From the commercial infant formula profiled in this study, a total of 154 peptides, corresponding to 31 unique proteins were identified by nano-HPLC/ESI/MS/MS. Each of the identified peptides was reconfirmed by a strict integrated approach using tandem mass spectra. This protein profiling method using gel electrophoresis coupled with nano-HPLC/ESI/MS/MS and manual evaluation is a sensitive and accurate method for protein identification as well as a powerful tool for monitoring various types of food products.
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 151742, Republic of Korea.
authors.forename: Soon Sun
authors.initials: SS
authors.lastname: Hong
authors.forename: Jeong Hill
authors.initials: JH
authors.lastname: Park
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kwon
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004044:Dietary Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005504:Food Analysis / Q000379:methods; D041943:Infant Formula / Q000737:chemistry*; D036103:Nanotechnology; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004044:Dietary Proteins