Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species.
bla(OXA-51-like) was sought in clinical isolates of Acinetobacter species in a multiplex PCR, which also detects bla(OXA-23-like) and class 1 integrase genes. All isolates that gave a band for bla(OXA-51-like) identified as A. baumannii. This gene was detected in each of 141 isolates of A. baumannii but not in those of 22 other Acinetobacter species.
Turton
Jane F
JF
Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, 61 Colindale Ave., London NW9 5EQ, United Kingdom. jane.turton@hpa.org.uk
Woodford
Neil
N
Glover
Judith
J
Yarde
Susannah
S
Kaufmann
Mary E
ME
Pitt
Tyrone L
TL
D040981:Acinetobacter baumannii / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D019426:Integrases / Q000235:genetics; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D019426:Integrases; D001618:beta-Lactamases; C063912:carbapenemase