Effects of etamsylate on equine platelets: in vitro and in vivo studies.
The aim of this study was to investigate whether etamsylate produces equine platelet activation. In vitro and in vivo studies were designed in which seven and eight adult healthy horses were included, respectively. In the in vitro study, citrated blood was incubated with different concentrations of etamsylate, and P-selectin expression and annexin V binding were determined by flow cytometry. In the in vivo study, blood was collected before and 1 and 2h after IV administration of etamsylate, and P-selectin expression was evaluated. In the in vitro study, a significant increase in P-selectin expression, leukocyte-platelet aggregate formation and annexin V binding were observed. In the in vivo study, a marked increase in P-selectin expression and heterotypic aggregate formation was seen in two and five horses, respectively, although no significant differences were detected when analyzing results from all the animals together. The results of the in vitro study indicate that etamsylate produces a pre-activation state in equine platelets, but this fact could be confirmed by the in vivo study.
Segura
Dídac
D
Servei de Medicina Interna Equina, Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Monreal
Luis
L
Pérez-Pujol
Sílvia
S
Alonso
Antonio
A
Díaz-Ricart
Maribel
M
Brugués
Rosa
R
Ordinas
Antonio
A
Escolar
Ginés
G
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004979:Ethamsylate / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary; D006490:Hemostatics / Q000494:pharmacology*; D006736:Horses / Q000097:blood* / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D015539:Platelet Activation / Q000187:drug effects*; D010976:Platelet Count / Q000662:veterinary
D017304:Annexin A5; D006490:Hemostatics; D019007:P-Selectin; D004979:Ethamsylate