Structural and functional behavior of biologically active monomeric melittin.
Melittin is a well-known water-soluble toxic peptide present in bee venom of Apis mellifera, capable of interacting with and disrupting cell membranes thus producing many effects on living cells. Additionally, melittin induces activation of phospholipases and calmodulin upon interaction with cellular membranes. The conformation and aggregation state adopted by melittin in solution depends on several factors including the peptide concentration, ionic strength, pH and the nature of the ions in the aqueous medium. Such conformational dependence on the peptide environment gives new insights over the currently available 3D structures of melittin and, ultimately, over its biologically functional unit. Based on crystallographic data, the melittin tetramer has been proposed as its bioactive form. Contrarily to such data, we show in this work the results obtained from molecular dynamics simulations, which clearly indicate that the tetrameric organization of melittin is not stable under biological conditions dissociating after 2.5 ns through a 10 ns trajectory. We found that the tetrameric form of melittin is stable only in conditions of high pH and high peptide concentration in the molecular dynamics simulations. Moreover, when in plasma melittin appears to be a random coil monomer, folding only upon interaction with biological membranes. In summary, these findings elucidate several properties of melittin structure and dynamics, projecting significant implications in the study of its biological function.
Terra
Renata M S
RM
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, CP 15005, Porto Alegre 91500-970, RS, Brazil.
Guimarães
Jorge A
JA
Verli
Hugo
H
D000818:Animals; D001514:Bee Venoms / Q000737:chemistry*; D001516:Bees / Q000737:chemistry*; D003198:Computer Simulation; D008555:Melitten / Q000737:chemistry*; D008958:Models, Molecular; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary
D001514:Bee Venoms; D008555:Melitten