Nanoparticle-based sensing of glycan-lectin interactions.
Here we present the first report on nanoparticle-based biosensing of glycan markers of diseases. The protocol relies on the competition between a nanocrystal (CdS)-tagged sugar and the target sugar for the binding sites of surface-confined lectin and monitoring the extent of competition through highly sensitive electrochemical detection of the captured nanocrystal. This development is expected to allow decentralized detection of carbohydrate moieties and lectin-carbohydrate interactions to be performed more rapidly, sensitively, inexpensively, and reliably.
Dai
Zong
Z
The Biodesign Institute and Fulton School of Engineering, Arizona State University, Tempe, Arizona 85287-5801, USA.
Kawde
Abdel-Nasser
AN
Xiang
Yun
Y
La Belle
Jeffrey T
JT
Gerlach
Jared
J
Bhavanandan
Veer P
VP
Joshi
Lokesh
L
Wang
Joseph
J
D037102:Lectins / Q000737:chemistry*; D053758:Nanoparticles; D011134:Polysaccharides / Q000737:chemistry*
D037102:Lectins; D011134:Polysaccharides