title: Expression analysis of CYP27B1 in tumor biopsies and cell cultures.
abstract: There is evidence for alternative splicing as a prevalent mechanism both in normal human cells and cancer cells. Alternative splice variants of the gene encoding the human 25-hydroxyvitamin-D3-1alpha-hydroxylase (CYP27B1) in human glioblastoma were recently reported. Using combined nested and touchdown PCR, full length CYP27B1 as well as several splice variants were cloned. Here, this approach was used to analyze the expression of CYP27B1 and its splice variants in normal tissues, tumor tissues and cell cultures. Expression of CYP27B1 was found in all tumor tissues. The expression of CYP27B1 was also found in several normal tissues including lung, kidney, placenta, thymus and stomach and in derived cell cultures. The expression of splice variants of CYP27B1 were detected in 1 out of 3 tested brain samples, with no evidence for the expression of normal CYP27B1.
authors.affiliation: Department of Human Genetics, The Saarland University Hospital, 66421 Homburg/Saar, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Radermacher
authors.forename: Britta
authors.initials: B
authors.lastname: Diesel
authors.forename: Markus
authors.initials: M
authors.lastname: Seifert
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Tilgen
authors.forename: Jörg
authors.initials: J
authors.lastname: Reichrath
authors.forename: Ulrike
authors.initials: U
authors.lastname: Fischer
authors.forename: Eckart
authors.initials: E
authors.lastname: Meese
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000096:biosynthesis* / Q000235:genetics; D017398:Alternative Splicing; D001706:Biopsy; D001921:Brain / Q000201:enzymology; D045744:Cell Line, Tumor; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D008545:Melanoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D009369:Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology
chemical_list: D007527:Isoenzymes; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase