Full validation of an analytical method for the HIV-protease inhibitor atazanavir in combination with 8 other antiretroviral agents and its applicability to therapeutic drug monitoring.
Because HIV medications are used in combination, it is important to develop multiplex assays to streamline the therapeutic drug monitoring process and provide rapid turnaround. This article reports full validation of an analytical method that combines atazanavir with 6 HIV-protease inhibitors (indinavir, amprenavir, saquinavir, nelfinavir, ritonavir, and lopinavir) and 2 nonnucleoside reverse transcriptase inhibitors (nevirapine and efavirenz). Using 200 microL of plasma and a simple liquid-liquid extraction method, this analytical method achieved a clean baseline and high extraction efficiencies (90.0% to 99.5%). A Zorbax C-18 (150 x 4.6 mm, 3.5 microm) analytical column was used along with a 27-minute linear gradient elution of the mobile phase to provide sharp peaks at 210 nm. This method was validated over a range of 25 to 10,000 ng/mL and is accurate (90.4% to 110.5%) and precise (precision within a day and between days ranged from 2.3% to 8.3%). Because this method is simple and inexpensive, it may have applicability in countries with low resources.
Rezk
Naser L
NL
Clinical Pharmacology/Analytical Chemistry Core, Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. naser2@unc.edu
Crutchley
Rustin D
RD
Yeh
Rosa F
RF
Kashuba
Angela D M
AD
D023241:Antiretroviral Therapy, Highly Active; D000069446:Atazanavir Sulfate; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D016903:Drug Monitoring / Q000191:economics / Q000379:methods*; D004355:Drug Stability; D017320:HIV Protease Inhibitors / Q000097:blood* / Q000627:therapeutic use; D006801:Humans; D015394:Molecular Structure; D009842:Oligopeptides / Q000097:blood* / Q000737:chemistry / Q000627:therapeutic use; D011725:Pyridines / Q000097:blood* / Q000737:chemistry / Q000627:therapeutic use; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions / Q000032:analysis / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D017320:HIV Protease Inhibitors; D009842:Oligopeptides; D011725:Pyridines; D012996:Solutions; D000069446:Atazanavir Sulfate