Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.
Rust
Michael J
MJ
Department of Physics, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.
Bates
Mark
M
Zhuang
Xiaowei
X
D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D036103:Nanotechnology / Q000379:methods; D013269:Stochastic Processes
D005456:Fluorescent Dyes; D004247:DNA