[ICCD-based fast fluorescence micro-imaging technique and preliminary application in living cells].
A fast fluorescence micro-imaging system using mainly intensified charge couple device (ICCD), argon-ion laser, and xenon lamp was set up, and its preliminary application in living cells was presented. Real-time observation and imaging of fast concentration and distribution changing of intracellular Ca2+ labeled by Fluo-3, a fluorescent Ca2+ indicator, in the proliferation process of rat cerebral micro-vessels endothelial cells (rCMECs) were carried out, and curves of artificial intensity versus imaging sequence of four typical points were obtained. It is shown that the ICCD-based fast fluorescence micro-imaging system is a powerful tool for recording the real-time fast processes in living cells.
Lin
Dan-Ying
DY
The Key Laboratory of Atomic and Molecular Nanosciences, Ministry of Education, Tsinghua University, Beijing, China.
Sun
Yun-Xu
YX
Ma
Wan-Yun
WY
Han
Dong
D
D000818:Animals; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D007090:Image Interpretation, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000379:methods*; D051381:Rats