[Relationship of genetic polymorphism in APE1 and ADPRT to risks of chronic benzene poisoning].
OBJECTIVE
To explore the relationship between genetic polymorphisms in apurinic/apyrimidinic endonuclease (APE1) and ADP ribosyltransferase (ADPRT) and individuals' susceptibility to chronic benzene poison ing (BP).


METHODS
A case-control study was conducted. One hundred and fifty-two B P patients and 152 workers occupationally exposed to benzene without poisoning manifestations were investigated. The mismatched bases combined to create restriction site with restrained fragment length polymorphism technique (CRS-RFLP) was used for detecting the single nucleotide polymorphisms (SNPs) at Asp148Glu of APE1 gene and Val762Ala of ADPRT gene.


RESULTS
There was no significant difference in the distribution of genotypes of APE1Asp148Glu and ADPRTVal762Ala between the patients and the control groups. Compared with individuals having genotype of APE1Asp148Glu T/T without habit of alcohol consumption, there was a 4.13 times increased risk of BP for the alcohol user with genotype of APE1Asp148Glu T/T (OR = 4.13, 95% CI: 1.07 - 15.85, P = 0.03). The analysis of Logistic regression showed that smoking may play some role in modifying the risk of cironic benzene poisoning (OR = 0.33, 95% CI: 0.14 - 0.75, P = 0.01).


CONCLUSIONS
The genetic polymorphisms in APE1Asp148Glu, ADPRTVal762Ala are not related to the risk of BP. Potential interaction is found between alcohol consumption and polymorphism of APE1Asp148Glu. Further study is needed to elucidate this interaction.
Sun
Pin
P
Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.
Zhang
Zhong-bin
ZB
Wan
Jun-xiang
JX
Jin
Xi-peng
XP
Xia
Zhao-lin
ZL
D036002:ADP Ribose Transferases; D000428:Alcohol Drinking / Q000235:genetics; D001554:Benzene / Q000506:poisoning*; D016022:Case-Control Studies; D002908:Chronic Disease; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
D036002:ADP Ribose Transferases; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D001554:Benzene