[Associated study on interleukin 10 gene promoter polymorphisms related to hepatitis B virus infection in Chinese Han population].
OBJECTIVE
To investigate the association that the polymorphisms of interleukin 10 gene (IL10) promoter region are related to the susceptibility and clinical phenotypes of hepatitis B virus (HBV) in Chinese Han population.


METHODS
With polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) method, the single nucleotide polymorphisms (SNP) of the promoter region of IL10 gene at position -1082G/A, -819T/C, -592A/C were detected in 231 patients with chronic hepatitis B, 165 individuals spontaneously recovered from HBV infection and 135 normal controls.


RESULTS
No significant difference was found in frequencies of genotypes and alleles of IL10 gene promoter region at position -1082G/A, -819T/C, -592A/C among normal controls, individuals spontaneously recovering from HBV infection and patients with chronic hepatitis B (P>0.05), also between patients with HBV infection with HBV-DNA<1x10(3)copies/mL and those with HBV-DNA> or =1 x 10(3)copies/mL (P>0.05). However, frequencies of TT genotype at position -819T/C and AA genotype at position -592A/C in chronic hepatitis B were significantly higher than that in asymptomatic HBV carriers (P<0.05).


CONCLUSIONS
It is possible that genetic polymorphisms of IL10 promoter region are not associated with both susceptibility of HBV infection and HBV-DNA replication after infected HBV in Chinese Han population. However, the polymorphisms of the promoter region IL10 at position -819T/C and -592A/C are related to inflammatory reaction to liver of the patients with HBV infection.
Zhang
Ping-an
PA
Department of Laboratory Science, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P. R. China. Zhangpingan@yahoo.com.cn
Li
Yan
Y
Yang
Xiang-sheng
XS
D044466:Asian People / Q000235:genetics; D002681:China; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006509:Hepatitis B / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*
D016753:Interleukin-10