title: Diagnostic tools based on minor groove binder probe technology for rapid identification of vaccinal and field strains of canine parvovirus type 2b.
abstract: TaqMan-based diagnostic tests have been developed for the identification of canine parvovirus type 2 (CPV-2) strains in the faeces of dogs with diarrhoea, including a minor groove binder (MGB) probe assay for identification of type 2-based vaccines and field strains (types 2a, 2b and 2c). Since type 2b vaccines have been licensed recently in Europe, two novel MGB assays were developed for discrimination between type 2b vaccines and field strains of CPV. Such assays have been found to be highly sensitive, specific and reproducible, allowing for simultaneous detection of type 2b vaccinal and field strains present in the same specimens. These new assays will help resolution of the diagnostic problems related to the detection of a type 2b strain in the faeces of dogs shortly after the administration of a type 2b vaccine.
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy. n.decaro@veterinaria.uniba.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Costantina
authors.initials: C
authors.lastname: Desario
authors.forename: Marco
authors.initials: M
authors.lastname: Campolo
authors.forename: Domenico
authors.initials: D
authors.lastname: Buonavoglia
authors.forename: Anna Lucia
authors.initials: AL
authors.lastname: Bellacicco
authors.forename: Maria
authors.initials: M
authors.lastname: Tempesta
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology; D015336:Molecular Probe Techniques; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology*; D017993:Parvovirus, Canine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014765:Viral Vaccines
chemical_list: D004279:DNA, Viral; D014765:Viral Vaccines