A plant peptide encoded by CLV3 identified by in situ MALDI-TOF MS analysis.
The Arabidopsis CLAVATA3 (CLV3) gene encodes a stem cell-specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applied to in situ Arabidopsis tissues determined the structure of a modified 12-amino acid peptide (MCLV3), which was derived from a conserved motif in the CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying the typical phenotype of transgenic plants overexpressing CLV3. These results suggest that the functional peptide of CLV3 is MCLV3.
Kondo
Tatsuhiko
T
Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
Sawa
Shinichiro
S
Kinoshita
Atsuko
A
Mizuno
Satoko
S
Kakimoto
Tatsuo
T
Fukuda
Hiroo
H
Sakagami
Youji
Y
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D006909:Hydroxyproline / Q000737:chemistry; D018519:Meristem / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013234:Stem Cells / Q000166:cytology
C118167:AT2G27250 protein, Arabidopsis; D029681:Arabidopsis Proteins; D009842:Oligopeptides; D006909:Hydroxyproline