title: Diffusion-limited patterning of molecules in nanofluidic channels.
abstract: Diffusion-limited patterning (DLP) is a new technique that enables patterning of labile molecular species in solution phase onto surfaces that are not easily accessible. This technique is self-aligning and is simple to implement for patterning multiple species. We demonstrated DLP by patterning alternating bands of fluorescently labeled and unlabeled streptavidin in biotin-functionalized nanofluidic channels with spatial resolution better than 1 microm. The methodology of DLP also enables experimental measurement of a unique parameter that relates molecular surface grafting density, concentration, diffusivity, and channel geometry.
authors.affiliation: Department of Mechanical Engineering, University of California, Berkeley, California 94720, USA.
authors.forename: Rohit
authors.initials: R
authors.lastname: Karnik
authors.forename: Kenneth
authors.initials: K
authors.lastname: Castelino
authors.forename: Chuanhua
authors.initials: C
authors.lastname: Duan
authors.forename: Arun
authors.initials: A
authors.lastname: Majumdar
mesh_terms: D003460:Crystallization / Q000379:methods*; D004058:Diffusion; D008422:Materials Testing; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D049329:Nanostructures / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D044367:Phase Transition; D016062:Porosity; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D013499:Surface Properties
chemical_list: D011506:Proteins; D012996:Solutions