title: [Usefulness of the molecular techniques for detecting and/or identifing of parasites and fungi in humans and animals or pathogens transmitted by ticks (Part I)].
abstract: After a long period of using basic microscopic, immunological and biochemical methods for diagnosis, rapid development of nucleic acids investigation enabled introduction of specific and sensitive methods of detection of pathogenic agents on the molecular level. Among others, polymerase chain reaction (PCR), discovered in mid of 80'ies and then automatized, offered an attractive alternative to conventional testing systems. In this paper we describe reliable diagnostic tests widely used in the world, including Poland, and capable of detecting different disease agents as parasites and fungi in clinical specimens and pathogens of emerging zoonotic diseases in ticks. The possibilities of using molecular methods for determination of Plasmodium falciparum drug resistance is also discussed. Moreover, the report offers information concerning kinds of molecular tests and institutions in which there are executed.
authors.affiliation: Zakład Parazytologii Tropikalnej, Instytut Medycyny Morskiej i Tropikalnej, 81-519 Gdynia, ul. Powstania Styczniowego 9 b.
authors.forename: P
authors.initials: P
authors.lastname: Myjak
authors.forename: A C
authors.initials: AC
authors.lastname: Majewska
authors.forename: A
authors.initials: A
authors.lastname: Bajer
authors.forename: E
authors.initials: E
authors.lastname: Siński
authors.forename: H
authors.initials: H
authors.lastname: Wedrychowicz
authors.forename: E
authors.initials: E
authors.lastname: Gołab
authors.forename: A
authors.initials: A
authors.lastname: Budak
authors.forename: J
authors.initials: J
authors.lastname: Stańczak
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000145:classification / Q000175:diagnosis*; D003458:Cryptosporidium / Q000145:classification; D021744:Cyclospora / Q000145:classification; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis; D004446:Echinococcus / Q000145:classification; D004747:Entamoeba / Q000145:classification; D006801:Humans; D016814:Microsporidia / Q000145:classification; D010271:Parasites / Q000145:classification / Q000302:isolation & purification*; D010272:Parasitic Diseases / Q000145:classification / Q000175:diagnosis*; D010961:Plasmodium / Q000145:classification; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D013987:Ticks / Q000145:classification / Q000382:microbiology* / Q000469:parasitology*; D014122:Toxoplasma / Q000145:classification; D014234:Trichinella / Q000145:classification
chemical_list: D015342:DNA Probes; D016054:DNA, Protozoan