title: Embryo-induced transcriptome changes in bovine endometrium reveal species-specific and common molecular markers of uterine receptivity.
abstract: The endometrium plays a central role among the reproductive tissues in the context of early embryo-maternal communication and pregnancy. This study investigated transcriptome profiles of endometrium samples from day 18 pregnant vs non-pregnant heifers to get insight into the molecular mechanisms involved in conditioning the endometrium for embryo attachment and implantation. Using a combination of subtracted cDNA libraries and cDNA array hybridisation, 109 mRNAs with at least twofold higher abundance in endometrium of pregnant animals and 70 mRNAs with higher levels in the control group were identified. Among the mRNAs with higher abundance in pregnant animals, at least 41 are already described as induced by interferons. In addition, transcript levels of many new candidate genes involved in the regulation of transcription, cell adhesion, modulation of the maternal immune system and endometrial remodelling were found to be increased. The different expression level was confirmed with real-time PCR for nine genes. Localisation of mRNA expression in the endometrium was shown by in situ hybridisation for AGRN, LGALS3BP, LGALS9, USP18, PARP12 and BST2. A comparison with similar studies in humans, mice, and revealed species-specific and common molecular markers of uterine receptivity.
authors.affiliation: Institute of Molecular Animal Breeding and Biotechnology, Ludwig-Maximilians University, Feodor-Lynen-Str. 25, 81377 Munich, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Bauersachs
authors.forename: Susanne E
authors.initials: SE
authors.lastname: Ulbrich
authors.forename: Karin
authors.initials: K
authors.lastname: Gross
authors.forename: Susanne E M
authors.initials: SE
authors.lastname: Schmidt
authors.forename: Heinrich H D
authors.initials: HH
authors.lastname: Meyer
authors.forename: Hendrik
authors.initials: H
authors.lastname: Wenigerkind
authors.forename: Margarete
authors.initials: M
authors.lastname: Vermehren
authors.forename: Fred
authors.initials: F
authors.lastname: Sinowatz
authors.forename: Helmut
authors.initials: H
authors.lastname: Blum
authors.forename: Eckhard
authors.initials: E
authors.lastname: Wolf
mesh_terms: D000818:Animals; D002417:Cattle; D010064:Embryo Implantation / Q000502:physiology*; D004717:Endometrium / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011323:Primates; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050794:STAT1 Transcription Factor / Q000235:genetics; D012756:Sheep; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D050794:STAT1 Transcription Factor