Tol2 transposon-mediated transgenesis in Xenopus tropicalis.
The diploid frog Xenopus tropicalis is becoming a powerful developmental genetic model system. Sequencing of the X. tropicalis genome is nearing completion and several labs are embarking on mutagenesis screens. We are interested in developing insertional mutagenesis strategies in X. tropicalis. Transposon-mediated insertional mutagenesis, once used exclusively in plants and invertebrate systems, is now more widely applicable to vertebrates. The first step in developing transposons as tools for mutagenesis is to demonstrate that these mobile elements function efficiently in the target organism. Here, we show that the Medaka fish transposon, Tol2, is able to stably integrate into the X. tropicalis genome and will serve as a powerful tool for insertional mutagenesis strategies in the frog.
Hamlet
Michelle R Johnson
MR
Department of Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Yergeau
Donald A
DA
Kuliyev
Emin
E
Takeda
Masatoshi
M
Taira
Masanori
M
Kawakami
Koichi
K
Mead
Paul E
PE
D000818:Animals; D001483:Base Sequence; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008845:Microinjections; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009990:Oryzias / Q000235:genetics; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D019076:Transgenes; D019895:Transposases / Q000235:genetics; D014981:Xenopus / Q000145:classification / Q000196:embryology / Q000235:genetics*
D006063:Chorionic Gonadotropin; D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins; D004247:DNA; D019895:Transposases; D003545:Cysteine