title: Hemorphin and hemorphin-like peptides isolated from dog pancreas and sheep brain are able to potentiate bradykinin activity in vivo.
abstract: Hemorphins are biologically active peptides, derived from hemoglobin, which presents a number of physiological activities. Proteolytic generation of these peptides is not fully understood; however, among their roles, is to provoke reduction on blood pressure. In this work, this particular biological effect was chosen as the monitor for the selection of mammalian vasoactive peptides. By combining high-performance liquid chromatography and mass spectrometry, including 'de novo' sequencing, several hemorphin-like peptides were identified presenting bradykinin potentiating activity. Moreover, taking LVV-hemorphin-7 as model compound, we evaluated its biological effect on blood pressure of anaesthetized rats. By summarizing all the results, it is possible to present the hemorphins as a family of proteolytically generated peptides that are able to potentiate bradykinin activity in vivo.
authors.affiliation: Center for Applied Toxinology, CAT-CEPID, Laboratório Especial de Toxinologia Aplicada, Instituto Butantan, Av. Vital Brasil 1500, Sao Paulo, SP 05503-900, Brazil.
authors.forename: Danielle
authors.initials: D
authors.lastname: Ianzer
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Konno
authors.forename: Carlos Henrique
authors.initials: CH
authors.lastname: Xavier
authors.forename: Reto
authors.initials: R
authors.lastname: Stöcklin
authors.forename: Robson Augusto S
authors.initials: RA
authors.lastname: Santos
authors.forename: Antônio Carlos Martins
authors.initials: AC
authors.lastname: de Camargo
authors.forename: Daniel Carvalho
authors.initials: DC
authors.lastname: Pimenta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D001794:Blood Pressure / Q000187:drug effects; D001920:Bradykinin / Q000819:agonists / Q000494:pharmacology / Q000502:physiology*; D001923:Brain Chemistry; D004285:Dogs; D004357:Drug Synergism; D005260:Female; D006168:Guinea Pigs; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D010179:Pancreas / Q000737:chemistry*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012756:Sheep; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D006454:Hemoglobins; D010446:Peptide Fragments; D010455:Peptides; C085122:hemorphin 7; D001920:Bradykinin