title: Nourseothricin acetyltransferease: a positive selectable marker for Toxoplasma gondii.
abstract: Molecular analysis of parasite genomes will require new molecular genetic tools. The nat1 gene of Streptomyces noursei encodes nourseothricin acetyltransferase, conferring resistance to the aminoglycoside antibiotic nourseothricin. Electroporation of nat1 cassettes into RH or Prugniaud strains of Toxoplasma gondii allows for selection of stable nourseothricin-resistant clones.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 53706, USA.
authors.forename: T
authors.initials: T
authors.lastname: Van Tam
authors.forename: Peggy J
authors.initials: PJ
authors.lastname: Rooney
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Knoll
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015139:Blotting, Southern; D004351:Drug Resistance / Q000235:genetics; D018274:Electroporation; D005819:Genetic Markers; D006801:Humans; D008297:Male; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics; D013309:Streptothricins / Q000494:pharmacology*; D014122:Toxoplasma / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D005819:Genetic Markers; D013309:Streptothricins; D000123:Acetyltransferases; C050473:nourseothricin acetyltransferase