title: Analysis of genetic variation in ribosomal DNA internal transcribed spacer and the NADH dehydrogenase subunit 4 mitochondrial genes of the onchocerciasis vector Simulium ochraceum.
abstract: Onchocerciasis is a serious disease vectored by black flies in the genus Simulium that are infected with the filarial parasite Onchocerca volvulus. In the Americas, black flies of the Simulium ochraceum s.l. species complex are important vectors of this parasite. Cytological studies have suggested that this species complex consists of at least three cytotypes that inhabit distinct habitats. In this study, the NADH dehydrogenase subunit four (ND4) and internal transcribed spacer (ITS) of the ribosomal RNA gene cluster were used to explore the degree of genetic diversity among S. ochraceum s.l. populations found in the three O. volvulus foci in Mexico. Both sequence regions were found to exhibit intra- and interpopulation variation. Four different ND4 alleles were found among the populations examined. Similarly, variation was noted in the ITS domain sequences within and among populations. Variation within the ITS sequence was primarily confined to a complex microsatellite locus. Four ITS length variants were observed, two of which were only seen in flies collected from the onchocerciasis focus in northern Chiapas. These data suggest that the ND4 and ITS sequences may prove to be useful markers for exploring interactions within and among the S. ochraceum s.l. populations in Mexico.
authors.affiliation: Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Blvd. del Maestro Esq. Elías Pińa, Col. Narciso Mendoza, 88710, Reynosa, Tamaulipas, México.
authors.forename: Mario A
authors.initials: MA
authors.lastname: Rodríguez-Pérez
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Núñez-González
authors.forename: Cristian
authors.initials: C
authors.lastname: Lizarazo-Ortega
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sánchez-Varela
authors.forename: Michael C
authors.initials: MC
authors.lastname: Wooten
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Unnasch
mesh_terms: D000818:Animals; D001483:Base Sequence / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D017344:Genes, Insect / Q000235:genetics; D050259:Genes, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D020180:Heteroduplex Analysis; D007303:Insect Vectors / Q000201:enzymology / Q000235:genetics*; D008800:Mexico; D018895:Microsatellite Repeats / Q000235:genetics; D009245:NADH Dehydrogenase / Q000235:genetics*; D009855:Onchocerciasis / Q000635:transmission; D016133:Polymerase Chain Reaction; D012843:Simuliidae / Q000201:enzymology / Q000235:genetics*
chemical_list: D017931:DNA Primers; D021903:DNA, Ribosomal Spacer; C098483:NADH dehydrogenase subunit 4; D009245:NADH Dehydrogenase