A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor I7.
In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor I7 (OR I7) in its membrane fraction. The OR I7 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy (EIS) measurements on samples and controls.
Hou
Yanxia
Y
Centre de Génie Electrique de Lyon (CEGELY), Ecole Centrale de Lyon, 69134 Ecully Cedex, France. yanxiahou24@yahoo.com
Jaffrezic-Renault
Nicole
N
Martelet
Claude
C
Zhang
Aidong
A
Minic-Vidic
Jasmina
J
Gorojankina
Tatiana
T
Persuy
Marie-Annick
MA
Pajot-Augy
Edith
E
Salesse
Roland
R
Akimov
Vladimir
V
Reggiani
Lino
L
Pennetta
Cecilia
C
Alfinito
Eleonora
E
Ruiz
Oscar
O
Gomila
Gabriel
G
Samitier
Josep
J
Errachid
Abdelhamid
A
D000818:Animals; D015374:Biosensing Techniques; D017097:Electric Impedance; D009812:Odorants / Q000032:analysis*; D015202:Protein Engineering; D051381:Rats; D018035:Receptors, Odorant; D013057:Spectrum Analysis
D018035:Receptors, Odorant; C509506:olfactory receptor I7, rat