Optothermal molecule trapping by opposing fluid flow with thermophoretic drift.
Thermophoresis moves molecules along temperature gradients, typically from hot to cold. We superpose fluid flow with thermophoretic molecule flow under well-defined microfluidic conditions, imaged by fluorescence microscopy. DNA is trapped and accumulated 16-fold in regions where both flows oppose each other. Strong 800-fold accumulation is expected, however, with slow trapping kinetics. The experiment is equally described by a three-dimensional and one-dimensional analytical model. As an application, we show how a radially converging temperature field confines short DNA into a 10 microm small spot.
Duhr
Stefan
S
Biophysics, Ludwig Maximilians Universität München, Amalienstrasse 54, 80799 München, Germany.
Braun
Dieter
D
D004247:DNA / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D013696:Temperature
D004247:DNA