title: Spectroscopic investigation of the function of aqueous 2-hydroxyethylmethacrylate/glutaraldehyde solution as a dentin desensitizer.
abstract: Fourier-transform (FT)-Raman and -infrared (IR) spectroscopy were employed to investigate the function of the aqueous 2-hydroxyethylmethacrylate/glutaraldehyde solution (Gluma) as a desensitizer. 2-Hydroxyethylmethacrylate (HEMA), glutaraldehyde (GA), and the mixture of HEMA/GA (i.e. Gluma) were used to interact with dentin, collagen, hydroxyapatite (HAP), and bovine serum albumin (BSA) individually. All the interactions were monitored by an FT-Raman spectrometer. FT-IR spectroscopy was also used in this study. The results show that HEMA could be absorbed by dentin and collagen; GA could cross-link collagen and BSA; and when BSA was added to Gluma, polymerization of HEMA occurred. The results suggest that Gluma acts as a desensitizer whereby, first, GA reacts with part of the serum albumin in dentinal fluid, which induces a precipitation of serum albumin, then, second, a reaction of GA with serum albumin induces polymerization of HEMA. The function of Gluma as a desensitizer to block dentinal tubules occurs via these two reactions.
authors.affiliation: National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
authors.forename: Chuangye
authors.initials: C
authors.lastname: Qin
authors.forename: Jingwei
authors.initials: J
authors.lastname: Xu
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000042:Absorption; D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation; D024042:Collagen Type I / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry; D003804:Dentin / Q000187:drug effects*; D003807:Dentin Sensitivity / Q000188:drug therapy*; D017886:Durapatite / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry / Q000627:therapeutic use*; D008689:Methacrylates / Q000737:chemistry / Q000627:therapeutic use*; D011108:Polymers / Q000737:chemistry; D012709:Serum Albumin / Q000187:drug effects; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D024042:Collagen Type I; D003432:Cross-Linking Reagents; D008689:Methacrylates; D011108:Polymers; D012709:Serum Albumin; C111486:gluma desensitizer; C005044:hydroxyethyl methacrylate; D017886:Durapatite; D005976:Glutaral