[Molecular methods and techniques used in diagnosis and epidemiology of infections caused by pathogenic fungi].
In this paper we reviewed the latest literature on molecular techniques used in diagnosis and epidemiology of infections caused by pathogenic fungi. Traditional methods used for the identification and typing of medically relevant fungi include morphological and biochemical analysis. These methods are time-consuming and base on phenotypic features what makes them unreliable. We described the usefulness in mycological studies of fast and very sensitive molecular methods which rely on PCR and hybridization techniques.
Dobrowolska
Anita
A
Zakład Genetyki Drobnoustrojów, Instytut Mikrobiologii i Immunologii, Uniwersytet Lódzki, ul. Banacha 12/16, 90-237 Lódź.
Bojarski
Lukasz
L
Staczek
Paweł
P
D016172:DNA Fingerprinting / Q000379:methods; D004271:DNA, Fungal / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D017720:Molecular Epidemiology; D016533:Mycological Typing Techniques; D009181:Mycoses / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique
D004271:DNA, Fungal