title: Analysis of secondary metabolites from eschscholtzia californica by high-performance liquid chromatography.
abstract: A rapid and precise analytical HPLC method has been developed for screening the major benzophenanthridine alkaloids produced by cell cultures of Eschscholtzia califomica, namely, sanguinarine, chelirubine, macarpine, chelerythrine and chelilutine. Separation was achieved on a C18, reversed-phase column with gradient elution using acetonitrile and 50 mM phosphoric acid. Detection was performed by both fluorescence (lambda(ex) 330 nm, lambda(em) 570 nm) and photodiode array, leading to good selectivity and precision in determining peak purity. A simple and quick sample preparation protocol was elaborated involving a methanolic extraction for the measurement of intracellular concentrations of the alkaloids and a solid phase extraction for their quantification in culture medium. Owing to the non-availability of commercially standards, a method for the purification of chelirubine, macar pine and chelilutine by semi-preparative HPLC was developed. Coupled together, the isolation method and the analytical method were highly reliable for screening the alkaloids of interest produced by E. califomica.
authors.affiliation: Department of Chemical Engineering, Ecole Polytechnique de Montréal, P.O. Box 6079 Centre-ville Station, Montréal, Québec, Canada H3C 3A7.
authors.forename: Maya
authors.initials: M
authors.lastname: Klvana
authors.forename: Jingkui
authors.initials: J
authors.lastname: Chen
authors.forename: François
authors.initials: F
authors.lastname: Lépine
authors.forename: Robert
authors.initials: R
authors.lastname: Legros
authors.forename: Mario
authors.initials: M
authors.lastname: Jolicoeur
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D053119:Benzophenanthridines; D002851:Chromatography, High Pressure Liquid; D031675:Eschscholzia / Q000737:chemistry* / Q000378:metabolism; D005470:Fluorometry; D007546:Isoquinolines; D010617:Phenanthridines / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000470:Alkaloids; D053119:Benzophenanthridines; D007546:Isoquinolines; D010617:Phenanthridines; C005705:sanguinarine; C016299:chelerythrine