Regulation of annexin I in rheumatoid synovial cells by glucocorticoids and interleukin-1.
The glucocorticoid (GC)-induced antiinflammatory molecule annexin I is expressed in leukocytes and has antiinflammatory effects in animal models of arthritis, but the expression of annexin I in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) is unknown. We report the constitutive and dexamethasone (DEX)-inducible expression of annexin I in RA FLS. DEX increased FLS annexin I protein translocation and mRNA expression. Interleukin (IL)-1beta also induced annexin I translocation and mRNA but also increased intracellular protein. DEX and IL-1 had additive effects on annexin I mRNA, but DEX inhibited the inducing effect of IL-1beta on cell surface annexin I. These results indicate that glucocorticoids and IL-1beta upregulate the synthesis and translocation of annexin I in RA FLS, but interdependent signalling pathways are involved.
Morand
Eric F
EF
Centre or Inflammatory Diseases, Monash Institute for Medical Research , Monash Medical Centre, Locked Bag No 29, Clayton Victoria 3168, Australia.
Hall
Pam
P
Hutchinson
Paul
P
Yang
Yuan H
YH
D017305:Annexin A1 / Q000096:biosynthesis*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D053583:Interleukin-1beta / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013583:Synovial Membrane / Q000166:cytology* / Q000378:metabolism*
D017305:Annexin A1; D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D007375:Interleukin-1; D053583:Interleukin-1beta; D012333:RNA, Messenger; D003907:Dexamethasone