Gene signature of breast cancer cell lines treated with lycopene.
Among the micronutrients studied in relation between nutrition and cancer, lycopene appears to be a breast cancer preventive phytochemical candidate found in raw tomatoes and tomato-derived products. In order to investigate the responsiveness of breast cancer genes to lycopene and to better understand the molecular mechanisms underlying the effects of lycopene, we used an oligonucleotide microarray approach. Human breast cancer cell lines (MCF-7 and MDA-MB-231) and a fibrocystic breast cell line (MCF-10a) were either exposed or not exposed to 10 microM lycopene for 48 h. Microarrays comprising 202 genes were used to identify genes responsive to lycopene supplementation. Hierarchical clustering revealed a cell line-specific lycopene modulation of breast cells. Based on the observed results, lycopene seems to exert regulation on apoptosis, cell cycle and DNA repair mechanisms according to estrogen and retinoic acid receptor cell status.
Chalabi
Nasséra
N
Centre Jean Perrin, Département d'Oncogénétique, 58 Rue Montalembert, BP 392, 63011 Clermont-Ferrand Cedex 01, France.
Delort
Laëtitia
L
Le Corre
Ludovic
L
Satih
Samir
S
Bignon
Yves-Jean
YJ
Bernard-Gallon
Dominique
D
D001943:Breast Neoplasms / Q000178:diet therapy / Q000188:drug therapy* / Q000235:genetics*; D002338:Carotenoids / Q000627:therapeutic use*; D006801:Humans; D000077276:Lycopene; D018551:Solanum lycopersicum; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D014407:Tumor Cells, Cultured
D002338:Carotenoids; D000077276:Lycopene