[Optimized culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides].
Culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides were optimized with single factor and uniform design experiment. The best medium recipe was found with 70 g/L glucose, 0.1 g/L (NH4)2SO4, 0.75 g/L yeast extract, 1.5 g/L MgSO4. 7H2O, 0.4g/L KH2PO4, sterilized at 121 degrees C for 15 min, and then supplemented with ZnSO4 1.91 x 10(-6) mmol/L, CaCl2 1.50 mmol/L, MnCl2 1.22 x 10(-4) mmol/L and CuSO4 1.00 x 10(-4) mmol/L. The optimal fermentation conditions were as follows: 50 mL of medium (pH 6.0) in 250 mL Erlenmeyer flask with 10% inoculum (28h) under orbital shaking at 200 r/min for 120h at 30 degrees C. Under these conditions, yeast biomass accumulated lipids up to 76.1%.
Li
Yong-Hong
YH
Department of Bioscience and Bioengineering, Dalian University of Technology, Dalian 116023, China.
Liu
Bo
B
Zhao
Zong-Bao
ZB
Bai
Feng-Wu
FW
D001487:Basidiomycota / Q000254:growth & development / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000096:biosynthesis*; D008278:Magnesium Sulfate / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D003470:Culture Media; D008055:Lipids; D008278:Magnesium Sulfate; D003300:Copper; D015032:Zinc