Behavioural response of Colorado potato beetle (Leptinotarsa decemlineata) larvae to selected plant extracts.
Potato leaves were treated with 2, 20 or 200 g kg(-1) solutions of extracts of five plant species collected in Turkey, and then exposed to larvae of Colorado potato beetle, Leptinotarsa decemlineata (Say). During the first 24 h of exposure, leaf consumption was not affected by 2 g kg(-1) extracts, whereas significantly more leaf tissue remained on leaves treated with 20 g kg(-1) extracts of Arctium lappa L., Bifora radians M Bieb, Humulus lupulus L. or Xanthium strumarium L. than on untreated control leaves. Feeding was not affected by the 20 g kg(-1) extract of Verbascum songaricum Schrenk ex Fisch & Mey. Extracts of all species at 200 g kg(-1) reduced larval feeding, with H. lupulus and X. strumarium providing the greatest protection. Observations of larval behaviour over the first 15 min of exposure to these extracts revealed that the interaction of beetles with leaf tissue was significantly affected by plant extracts. Feeding frequency was not affected by 2 g kg(-1) extracts but was reduced by all higher concentrations. Feeding was inhibited completely by 20 g kg(-1) of H. lupulus extract and reduced significantly compared with the controls by all other extracts. Suppression of feeding was caused by all extracts at 200 g kg(-1), with V. songaricum providing 91% reduction in feeding duration. Rejection behaviour, in which larvae did not return to the leaf after their interaction with it, was rare on 2 g kg(-1) extracts but seen in over 60% of larvae on 20 g kg(-1) extracts and over 80% on 200 g kg(-1) extracts. The present results demonstrate that these extracts have significant ability to protect potato leaves for up to 24 h by prevention of feeding behaviour by L. decemlineata. Further studies are needed to determine the potential of these plant extracts, or their active components, for use in biologically based pest management strategies.
Gökçe
Ayhan
A
Department of Plant Protection, Agriculture Faculty, Gaziosmanpasa University, Tokat, Turkey. agokce@gop.edu.tr
Isaacs
Rufus
R
Whalon
Mark E
ME
D000818:Animals; D019661:Apiaceae / Q000737:chemistry; D031170:Arctium / Q000737:chemistry; D001517:Coleoptera / Q000187:drug effects*; D005247:Feeding Behavior / Q000187:drug effects*; D027582:Humulus / Q000737:chemistry; D007302:Insect Repellents / Q000032:analysis; D007814:Larva / Q000187:drug effects*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves; D013997:Time Factors; D032265:Verbascum / Q000737:chemistry; D031210:Xanthium / Q000737:chemistry
D007302:Insect Repellents; D010936:Plant Extracts