[Application of rat tail collagen in the primary culture of rats marginal cells of stria vascularis].
OBJECTIVE
To study the feasibility of application of rat-tail collagen in the primary culture of rats marginal cells of stria vascularis.


METHODS
The effect of self-made rat-tail collagen on the primary culture of rats marginal cells of stria vascularis was observed and estimated.


RESULTS
When cochlear stria vascularis fragment was isolated and cultured for 24 h, a few culture cells appeared around the fragments. About 48 to approximately 72 h later, clusters of culture cells could be seen,these cells showed the cobblestone shape under the microscope. The immunohistochemistry and the transmission electron microscopy showed the epithelial origination of these cells.


CONCLUSIONS
It is feasible to the primarily culture the rats marginal cells of stria vascularis with self-made rat-tail collagen.
Yan
Kaisheng
K
Department of Otorhinolaryngology, Union Hospital, Huanzhong University of Science and Technology, Wuhan, 430022, China.
Luo
Linghui
L
Fu
Yong
Y
Chen
Guangli
G
Gong
Shusheng
S
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003051:Cochlea / Q000166:cytology; D003094:Collagen / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013316:Stria Vascularis / Q000166:cytology* / Q000187:drug effects*
D003094:Collagen