Supplementation of the cultivation media with B-group vitamins enhances lovastatin biosynthesis by Aspergillus terreus.
The impact of the supplementation of cultivation media with B-group vitamins on the biosynthesis of lovastatin (mevinolinic acid) by Aspergillus terreus ATCC20542 was investigated. A hypothesis was formulated that as the biosynthesis of lovastatin requires a high throughput of coenzymes in the cells, the application of its precursors in the form of B-group vitamins might positively influence the process. In a nitrogen-deficient medium the B-group vitamins, both single, especially nicotinamide, pyridoxine and calcium D-pantothenate, and a mixture of thiamine, riboflavin, pyridoxine, calcium d-pantothenate and nicotinamide increased the efficiency of lovastatin biosynthesis. The vitamin supplementation also increased both volumetric and specific production rates of mevinolinic acid, especially before 80 h of the process, when no lactose limitation had been observed yet.
Bizukojc
Marcin
M
Department of Bioprocess Engineering, Technical University of Lodz, ul. Wolczanska 213/215, 90-924 Lodz, Poland. marcin.bizukojc@p.lodz.pl
Pawlowska
Beata
B
Ledakowicz
Stanislaw
S
D001230:Aspergillus / Q000187:drug effects* / Q000378:metabolism*; D018533:Biomass; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D008148:Lovastatin / Q000096:biosynthesis*; D013997:Time Factors; D014803:Vitamin B Complex / Q000494:pharmacology*; D015003:Yeasts
D003470:Culture Media; D014803:Vitamin B Complex; D008148:Lovastatin; D007785:Lactose