title: [Occurrence of Babesia microti in ticks Ixodes ricinus on selected areas of western Pomerania].
abstract: The aim of present study was to evaluate acquisition risk of babesiosis in human population exposed to ticks Ixodes ricinus by examination of Babesia microti DNA occurrence in ticks of all development stages. The polymerase chain reaction (PCR) was applied to estimate the occurrence of DNA Babesia microti in Ixodes ricinus. The Bab1 and Bab4 primers were used to amplify fragment. 238bp in length, of 18S rRNA gene for small ribosomal subunit. Amplicons were electroforeticaly separated in agarose gels. Ticks were collected in year 1999 and 2000, twice in each year in spring-summer (May-July) and summer-autumn (August-October) seasons from Goleni6w Forest and Pobierowo. These places have been classified as people attendance and tourist areas. The 716 I. ricinus ticks were collected in 1999 with 61.3% of nymphs, 17.8% larvae, 10.9% females and 9.9% males. Highest range of infection was observed in females--28.8% studied, than males--18.3%, nymphs--7.7% and larvae--3.l1%. The total number of 416 I. ricinus was collected in year 2000 with 64% of nymphs. 13.4%/males. 11.9% females and 10.7% larvae. The infection with Babesia microtri occurred only in three nymphs, which was 0.7% of studied population.
authors.affiliation: Katedra Genetyki, Wydział Nauk Przyrodniczych, Uniwersytet Szczeciński, Al. Piastów 40B, 71-065 Szczecin. Skotarczak@sus.univ.szczecin.pl
authors.forename: Bogumiła
authors.initials: B
authors.lastname: Skotarczak
authors.forename: Marek
authors.initials: M
authors.lastname: Sawczuk
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology; D041001:Babesia microti / Q000145:classification / Q000302:isolation & purification*; D001404:Babesiosis / Q000175:diagnosis* / Q000453:epidemiology*; D005260:Female; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D007814:Larva / Q000382:microbiology; D008297:Male; D009758:Nymph / Q000382:microbiology; D011044:Poland / Q000453:epidemiology; D015995:Prevalence; D012189:Retrospective Studies; D012621:Seasons; D014197:Trees / Q000469:parasitology*