title: Pharmacokinetics of chlorhexidine gluconate 0.02% in the rabbit cornea.
abstract: OBJECTIVE
  The aim of this study was to determine the pharmacokinetic parameters of chlorhexidine gluconate (CHG) in the rabbit cornea.
  
  
  METHODS
  Each eye of 16 New Zealand white rabbits were topically instilled with 50 microL of CHG 0.02% eye drops twice with a 5-min interval. Four (4) corneas of 2 rabbits were harvested at each time point. The concentration of CHG in the cornea was determined with high-performance liquid chromatography (HPLC) and 387 software to simulate the pharmacokinetic parameters.
  
  
  RESULTS
  The concentration of CHG in the cornea displayed an open two-compartment model. Tmax was 13.75 min, Cmax 0.713 microg.g1, clearance rate 1.64 microg.g-1.min-1, and t1/2alpha, t1/2beta, and t1/2ka was 2.65, 48.72, and 2.67 min, respectively.
  
  
  CONCLUSIONS
  The concentration of CHG in the rabbit cornea could be determined by means of HPLC. The maximum concentration of CHG in the corneal tissue was much higher than the trophozoite minimum amoebicidal concentration (TMAC) in vitro.
authors.affiliation: Department Ocular Microbiology, Beijing Institute of Ophthalmology, Beijing Tongren Hospital and Capital University of Medical Science, Beijing, China. sunxuguang@yahoo.com
authors.forename: Sun
authors.initials: S
authors.lastname: Xuguang
authors.forename: Liang
authors.initials: L
authors.lastname: Yanchuang
authors.forename: Zhang
authors.initials: Z
authors.lastname: Feng
authors.forename: Luo
authors.initials: L
authors.lastname: Shiyun
authors.forename: Yin
authors.initials: Y
authors.lastname: Xiaotang
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000493:pharmacokinetics*; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D003315:Cornea / Q000378:metabolism*; D009883:Ophthalmic Solutions / Q000493:pharmacokinetics; D011817:Rabbits; D014018:Tissue Distribution
chemical_list: D000891:Anti-Infective Agents, Local; D009883:Ophthalmic Solutions; C010882:chlorhexidine gluconate; D002710:Chlorhexidine