title: Muscle regeneration by adipose tissue-derived adult stem cells attached to injectable PLGA spheres.
abstract: The [corrected] use of adult stem cells for cell-based tissue engineering and regeneration strategies represents a promising approach for skeletal muscle repair. We have evaluated the combination of adipose tissue-derived adult stem cells (ADSCs) obtained from autologous liposuction and injectable poly(lactic-co-glycolic acid) (PLGA) spheres for muscle regeneration. ADSCs attached to PLGA spheres and PLGA spheres alone were cultured in myogenic medium for 21 days and injected subcutaneously into the necks of nude mice. After 30 and 60 days, the mice were sacrificed, and newly formed tissues were analyzed by immunostaining, H and E staining, and RT-PCR. We found that ADSCs attached to PLGA spheres, but not PLGA spheres alone, were able to generate muscle tissue. These findings suggest that ADSCs and PLGA spheres are useful materials for muscle tissue engineering and that their combination can be used in clinical settings for muscle regeneration.
authors.affiliation: Asan Institute for Life Sciences, University of Ulsan, Seoul, Republic of Korea. mjkimhsc@korea.com
authors.forename: MiJung
authors.initials: M
authors.lastname: Kim
authors.forename: Yu Suk
authors.initials: YS
authors.lastname: Choi
authors.forename: Seung Hye
authors.initials: SH
authors.lastname: Yang
authors.forename: Hea-Nam
authors.initials: HN
authors.lastname: Hong
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Sang Myun
authors.initials: SM
authors.lastname: Cha
authors.forename: Jhang Ho
authors.initials: JH
authors.lastname: Pak
authors.forename: Chan Wha
authors.initials: CW
authors.lastname: Kim
authors.forename: Seog Woon
authors.initials: SW
authors.lastname: Kwon
authors.forename: Chan Jeoung
authors.initials: CJ
authors.lastname: Park
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D006801:Humans; D019344:Lactic Acid; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D009132:Muscles / Q000502:physiology*; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D012038:Regeneration; D033581:Stem Cell Transplantation / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid