title: [Effects and significance of gas explosion on expression of NF-kappaB and ICAM1 in lung tissue of rats].
abstract: OBJECTIVE
  To explore the expression of NF-kappaB and ICAM-1 in the gas explosion wounded lung of rats and the relationship.
  
  
  METHODS
  Digoxin labeled NF-kappaB was used as probe. In situ hybridization was performed to detect the NF-kappaB mRNA. Immunohistochemistry was used to detect the expression of NF-kappaB and ICAM-1.
  
  
  RESULTS
  The levels of NF-kappaB mRNA, the expression of NF-kappaB and ICAM-1 in the wounded rats were significantly increased and reached their peak two hours after injury. Pathology of lung tissue showed that some crachea epithelium mucosae were desquamated; congestion, edema of trachea wall and infiltration of neutrophilic granulocytes were found; hemorrhage, edema and infiltration of lots of inflammatory cells were present in alveolus cells. Electron microscope showed that type I, especially type II alveolus epithelia had degeneration and desquamation.
  
  
  CONCLUSIONS
  The injury of gas explosion can activate NF-kappaB, which has close correlation with the acute injury to lung.
authors.affiliation: General Hospital of Coal Mining Group of Pingdingshan, Henan Province 467000, China.
authors.forename: Lin-hong
authors.initials: LH
authors.lastname: Liu
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Ren
authors.forename: Gui-zhen
authors.initials: GZ
authors.lastname: Wang
authors.forename: Zu-xuan
authors.initials: ZX
authors.lastname: Qin
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Wang
authors.forename: Song-yuan
authors.initials: SY
authors.lastname: Wu
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.forename: Liang-ming
authors.initials: LM
authors.lastname: Liu
mesh_terms: D000818:Animals; D001753:Blast Injuries / Q000378:metabolism* / Q000473:pathology; D005107:Explosions; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1