Determination and pharmacokinetic study of syringin and chlorogenic acid in rat plasma after administration of Aidi lyophilizer.
A high-performance liquid chromatographic (HPLC) method was developed for the first time to simultaneously quantify syringin and chlorogenic acid in rat plasma using wavelength-transfer technology. The analysis was performed on a Diamonsil C(18) column (200 x 4.6 mm i.d., 5 microm particle size) with isocratic mobile phase consisting of acetonitrile-0.05% phosphoric acid (12:88, v/v). The linear ranges were 0.20-10 and 0.25-30 microg/mL, respectively. The lower limits of quantification were 0.20 and 0.25 microg/mL, respectively. The method was shown to be reproducible and reliable with intraday precision below 8.5 and 6.1%, interday precision below 7.1 and 5.5%, accuracy within +/-7.1 and +/-8.6%, and mean extraction recovery excess of 92.1 and 80.9%, respectively, which were all calculated from the blank plasma sample spiked with syringin and chlorogenic acid at three concentrations of 0.20, 1.0 and 6.0 microg/mL for syringin and 0.25, 2.0 and 20 microg/mL for chlorogenic acid. This method was validated for specificity, accuracy and precision and was successfully applied to the pharmacokinetic study of syringin and chlorogenic acid in rat plasma after intravenous administration of Aidi lyophilizer.
Li
Qing
Q
School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, Liaoning Province, People's Republic of China.
Sun
Li-Xin
LX
Xu
Liang
L
Jia
Ying
Y
Wang
Zhi-Wei
ZW
Shen
Zhen-Duo
ZD
Bi
Kai-Shun
KS
D000818:Animals; D002726:Chlorogenic Acid / Q000097:blood / Q000493:pharmacokinetics*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D010666:Phenylpropionates / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D014466:Ultraviolet Rays
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010666:Phenylpropionates; C517300:aidi herbal drug; D002726:Chlorogenic Acid; C028305:syringin