title: Interleukin-3 and c-Kit/stem cell factor are required for normal eosinophil responses in mice infected with Strongyloides venezuelensis.
abstract: To evaluate the potential roles of Interleukin-3 (IL-3) and c-Kit, the tyrosine kinase receptor for stem cell factor (SCF), in eosinophil responses in vivo, we examined eosinophil numbers in uninfected or nematode-infected wild-type mice, IL-3-/- mice, and IL-3-/- mice that also have a marked reduction in SCF/c-Kit signaling (ie, Kit(W)/Kit(W-v), IL-3-/- mice). We found no significant differences in the numbers of eosinophils in the blood, bone marrow or various tissues of IL-3-/- vs IL-3+/+ mice, either at baseline or after the induction of bone marrow, blood or tissue eosinophilia in response to infection with Strongyloides venezuelensis (S.v.) or Nippostrongylus brasiliensis (N.b.). However, in mice with markedly impaired SCF/c-Kit signaling, IL-3 contributed significantly to the increased numbers of eosinophils that were observed in multiple tissues during S.v. infection, but not during infection with N.b.
authors.affiliation: Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA.
authors.forename: Koichi
authors.initials: K
authors.lastname: Kimura
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Song
authors.forename: Akhil
authors.initials: A
authors.lastname: Rastogi
authors.forename: Glenn
authors.initials: G
authors.lastname: Dranoff
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Galli
authors.forename: Chris S
authors.initials: CS
authors.lastname: Lantz
mesh_terms: D000818:Animals; D001491:Basophils / Q000276:immunology / Q000469:parasitology; D004195:Disease Models, Animal; D004802:Eosinophilia / Q000276:immunology / Q000469:parasitology; D007377:Interleukin-3 / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology / Q000469:parasitology; D051379:Mice; D018345:Mice, Knockout; D009559:Nippostrongylus / Q000276:immunology; D019009:Proto-Oncogene Proteins c-kit / Q000276:immunology*; D015398:Signal Transduction; D019089:Stem Cell Factor / Q000276:immunology; D013321:Strongyloides; D013322:Strongyloidiasis / Q000276:immunology*
chemical_list: D007377:Interleukin-3; D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit