title: Bacteroides fragilis adherence to Caco-2 cells.
abstract: The ability of ten Bacteroides fragilis strains isolated from intestinal and non-intestinal infections, normal flora and environment to adhere to human colon carcinoma cells, Caco-2, was examined. The adherence capacity varied among the strains tested from strongly adherent (76-100%) to non- or weakly adherent (0-25%). Negative staining with Indian ink showed that all the strains were capsulated, although strain 1032 (strongly adherent and originated from bacteremia) had the highest rate of capsulated cells in the culture. All strains studied presented an electron-dense layer and no fimbrial structures in their surface after PTA negative staining. The analysis of the strains with ruthenium red showed the presence of an acidic polysaccharide and also surface vesicles in all of them. The strain 1032 presented an aggregative adherence pattern toward Caco-2 cells monolayers. It could be seen trapped by elongated microvilli and surrounded by extracellular material in the scanning electron microscope. Treatment with sodium periodate (100 mM/1 h) reduced significantly its adherence capacity and also the expression of an electron-dense layer and of the capsule, detected with PTA and Indian ink staining, respectively. We suggest that the capsular polysaccharide might mediate the adherence of the B. fragilis to Caco-2 cells.
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Góes, UFRJ, Av. Brigadeiro Trompovsky, s/n, CCS, Bloco I, 2o andar, sala 6, Ilha do Fundão, Rio de Janeiro 21941-590, Brazil.
authors.forename: E O
authors.initials: EO
authors.lastname: Ferreira
authors.forename: L S
authors.initials: LS
authors.lastname: Falcão
authors.forename: D C
authors.initials: DC
authors.lastname: Vallim
authors.forename: F J
authors.initials: FJ
authors.lastname: Santos
authors.forename: J R C
authors.initials: JR
authors.lastname: Andrade
authors.forename: A F B
authors.initials: AF
authors.lastname: Andrade
authors.forename: R C
authors.initials: RC
authors.lastname: Vommaro
authors.forename: M C S
authors.initials: MC
authors.lastname: Ferreira
authors.forename: R M C P
authors.initials: RM
authors.lastname: Domingues