No correlation of five gene polymorphisms with periodontal conditions in a Greek population.
BACKGROUND
Various studies have examined possible correlations between a number of cytokine gene polymorphisms and periodontal disease in populations of different origins. The present study sought the correlation between four single-nucleotide polymorphisms (IL1A+3954, IL1B+4845, TNFA-308, COL1A1 Sp1), a variable number of tandem repeats polymorphism (IL1RN intron 2) and periodontal conditions in subjects of Greek origin.


METHODS
One hundred and ninety-two healthy subjects, stratified as non-periodontitis and periodontitis (chronic and aggressive) cases, participated in the present study. Genotyping was performed by polymerase chain reaction-based techniques using the primers and conditions described in the literature. The frequencies of genotypes between study groups were compared using Genepop v3.3 genetic software and Instat statistical package.


RESULTS
No differences were observed among the groups concerning the distributions of genotypes under investigation.


CONCLUSIONS
Carriage rates of the polymorphisms under investigation in systemically healthy subjects of Greek origin are well within the range reported for Caucasians but these polymorphisms cannot discriminate between non-periodontitis and periodontitis (chronic or aggressive) cases.
Sakellari
D
D
Department of Preventive Dentistry, Periodontology, and Implant Biology, Dental School, Aristotle University of Thessaloniki, Thessaloniki, Greece. dimisak@med.auth.gr
Katsares
V
V
Georgiadou
M
M
Kouvatsi
A
A
Arsenakis
M
M
Konstantinidis
A
A
D000328:Adult; D016022:Case-Control Studies; D002908:Chronic Disease; D024042:Collagen Type I / Q000235:genetics; D000090602:Collagen Type I, alpha 1 Chain; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006115:Greece; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics; D053582:Interleukin-1alpha / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D007438:Introns / Q000235:genetics; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D010518:Periodontitis / Q000145:classification / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D016207:Cytokines; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha