Toxoplasma gondii: comparison of human CD34+ and monocyte-derived dendritic cells after parasite infection.
Human dendritic cells (DC) obtained in vitro from CD34(+) progenitors (CD34-DC) or blood monocytes (mo-DC) are different DC which may be used in a model of T. gondii infection. We compared the survival, infection rate and cell surface receptor expression of both DC types after living T. gondii tachyzoite infection. CD34-DC appeared less resistant to the parasite than mo-DC. At 48h post-infection, chemokine receptors responsible for DC homing and migration were absent in mo-DC, while down regulation of CCR6 and up regulation of CCR7 was observed in CD34-DC. This result, suggesting migration ability of CD34-DC, was confirmed by in vitro migration experiments against different chemokines. Tachyzoite supernatant, used as chemokine, attracted immature CD34-DC as observed by MIP3alpha, while MIP3beta, as expected, attracted mature CD34-DC. Under similar conditions, no significant difference was noticed between mature or immature mo-DC. These data indicated that CD34-DC represent an alternative model that allows migration assay of infected DC by T. gondii.
Persat
F
F
Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard, Lyon 1, EA 37-32, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France. florence.persat@univ-lyon1.fr
Diana
J
J
Benadiba
C
C
Ferrandiz
J
J
Péguet-Navarro
J
J
Peyron
F
F
Picot
S
S
Schmitt
D
D
Vincent
C
C
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis* / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000469:parasitology; D005260:Female; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D019707:Receptors, Chemokine / Q000378:metabolism; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000097:blood / Q000276:immunology*
D018952:Antigens, CD34; D019707:Receptors, Chemokine