Cachexia--induced cerebellar degeneration: involvement of serum TNF and MCP-1 in the course of experimental neoplastic disease.
Cerebellar degeneration may be recognized as a remote effect of a growing tumor. We have analysed serum concentrations of tumor necrosis factor-alpha (TNF-alpha), macrophage chemoattractant protein-1 (MCP-1), thyroxine and insulin to elucidate the pathomechanism which may be of importance for the development of central degeneration in cachectic Morris hepatoma bearing rats. Serum TNF-alpha and MCP-1 levels were evaluated by means of the ELISA system, while thyroxine and insulin were estimated by radioimmunoassay. Microscopic examination using hematoxylin-eosin, Nissl and Klüver-Barrera staining revealed an atrophy in the cerebellum, homogenization changes of Purkinje cells and decreased cell density of the granular layer. In the Morris hepatoma bearing animals serum MCP-1 content was elevated while TNF-alpha, thyroxine and insulin concentrations were decreased. This study has demonstrated that circulating TNF-alpha and MCP-1, together with decreased levels of insulin and thyroxine accompany and may produce a milieu of factors involved in mechanisms of the development of cerebellar degeneration in cachectic hepatoma bearing rats.
Michalak
Sławomir
S
Department of Neurochemistry and Neuropathology, Chair of Neurology, University of Medical Sciences, 49 Przybyszewskiego St. 60-355 Poznań, Poland. slamic@yahoo.com.
Wender
Mieczysław
M
Michałowska-Wender
Grazyna
G
D000818:Animals; D001284:Atrophy; D002100:Cachexia / Q000209:etiology* / Q000378:metabolism; D018932:Chemokine CCL2 / Q000097:blood*; D007328:Insulin / Q000097:blood; D008113:Liver Neoplasms / Q000150:complications* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000150:complications* / Q000378:metabolism; D008297:Male; D009368:Neoplasm Transplantation; D020362:Paraneoplastic Cerebellar Degeneration / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D011689:Purkinje Cells / Q000473:pathology; D051381:Rats; D011915:Rats, Inbred BUF; D013974:Thyroxine / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D007328:Insulin; D014409:Tumor Necrosis Factor-alpha; D013974:Thyroxine