Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana.
Thiamin pyrophosphate is an essential coenzyme in all organisms that depend on fermentation, respiration or photosynthesis to produce ATP. It is synthesized through two independent biosynthetic routes: one for the synthesis of 2-methyl-4-amino-5-hydroxymethylpyrimidine pyrophosphate (pyrimidine moiety) and another for the synthesis of 4-methyl-5-(beta-hydroxyethyl) thiazole phosphate (thiazole moiety). Herein, we will describe the three-dimensional structure of THI1 protein from Arabidopsis thaliana determined by single wavelength anomalous diffraction to 1.6A resolution. The protein was produced using heterologous expression in bacteria, unexpectedly bound to 2-carboxylate-4-methyl-5-beta-(ethyl adenosine 5-diphosphate) thiazole, a potential intermediate of the thiazole biosynthesis in Eukaryotes. THI1 has a topology similar to dinucleotide binding domains and although details concerning its function are unknown, this work provides new clues about the thiazole biosynthesis in Eukaryotes.
Godoi
Paulo H C
PH
Departamento de Física e Informática, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP, CP 369, 13560-970, Brazil.
Galhardo
Rodrigo S
RS
Luche
Douglas D
DD
Van Sluys
Marie-Anne
MA
Menck
Carlos F M
CF
Oliva
Glaucius
G
D000255:Adenosine Triphosphate / Q000737:chemistry; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D004926:Escherichia coli / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013835:Thiamine Pyrophosphate / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
D029681:Arabidopsis Proteins; C437085:THI1 protein, Arabidopsis; D013844:Thiazoles; D000255:Adenosine Triphosphate; D013835:Thiamine Pyrophosphate