title: [Expression of multi-copy and immuno-reactivity of recombinant type-specific epitope of herpes simplex virus type 1].
abstract: OBJECTIVE
  To express a multi-copy specific epitope recombinant protein of herpes simplex virus type 1 (HSV-1) with immuno-reactivity.
  
  
  METHODS
  Multi-copy genes with a specific epitope of HSV-1-glycoprotein G 112-127 were constructed by DNA recombination and cloned in E. coli JM109 pGEM-5Zf. The positive recombinants were determined by SDS-PAGE and Western blotting.
  
  
  RESULTS
  The recombinants with 4, 8, 16 and 32 copies of gG 112-127 were obtained. The 8-copy recombinant was expressed by 17.5%, mainly as inclusion body. And it reacted with antiserum HSV-1, but not with antiserum HSV-2.
  
  
  CONCLUSIONS
  The HSV-1-gG112-127 recombinant could be used to distinguish HSV-1 and HSV-2 in ELISA.
authors.affiliation: Department of Clinical Microbiology and Immunology, the Third Military Medical University and Chongqing Research Center of Biopharmaceutical Engineering Technology, Chongqing 400038, China.
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Ji
authors.forename: Xu-hu
authors.initials: XH
authors.lastname: Mao
authors.forename: Quan-ming
authors.initials: QM
authors.lastname: Zou
authors.forename: Qing-tan
authors.initials: QT
authors.lastname: Yu
authors.forename: Li-li
authors.initials: LL
authors.lastname: Zhao
mesh_terms: D000937:Antigen-Antibody Reactions; D004274:DNA, Recombinant; D000939:Epitopes / Q000096:biosynthesis* / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D004274:DNA, Recombinant; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins