title: Pin1 gene mutation is a rare event in gastric cancer.
abstract: The peptidyl-prolyl isomerase Pin1 is strikingly overexpressed in human cancers and is a novel regulator of beta-catenin. To determine whether somatic mutation of the Pin1 gene is involved in the development and/or progression of gastric cancer, we searched for mutations of the Pin1 gene in 95 gastric cancer specimens. The effect of Pin1 on beta-catenin expression was further examined in wild- and mutant-type Pin1-transfected HEK 293T cells. We found only one missense mutation that led to the substitution of alanine by aspartic acid at codon 118 of the Pin1 gene. On transfection study, the mutant Pin1 showed an increased expression of beta-catenin. However, the mutation had no effect on expression of the Pin1 protein in the case with Pin1 mutation. These results suggest that Pin1 may not play a role in the development or progression of gastric cancer.
authors.affiliation: Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Chang Jae
authors.initials: CJ
authors.lastname: Kim
authors.forename: Jae Hwi
authors.initials: JH
authors.lastname: Song
authors.forename: Yong Gu
authors.initials: YG
authors.lastname: Cho
authors.forename: Hiun Suk
authors.initials: HS
authors.lastname: Chae
authors.forename: Suk Woo
authors.initials: SW
authors.lastname: Nam
authors.forename: Nam Jin
authors.initials: NJ
authors.lastname: Yoo
authors.forename: Jung Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Won Sang
authors.initials: WS
authors.lastname: Park
mesh_terms: D002460:Cell Line; D004252:DNA Mutational Analysis; D018450:Disease Progression; D006801:Humans; D016297:Mutagenesis, Site-Directed; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D019696:Peptidylprolyl Isomerase / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D018807:Polymorphism, Single-Stranded Conformational; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D000072340:NIMA-Interacting Peptidylprolyl Isomerase; C000606846:PIN1 protein, human; D019696:Peptidylprolyl Isomerase