Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination.
DNA double-strand breaks (DSB) can arise during DNA replication, or after exposure to DNA-damaging agents, and their correct repair is fundamental for cell survival and genomic stability. Here, we show that the Smc5-Smc6 complex is recruited to DSBs de novo to support their repair by homologous recombination between sister chromatids. In addition, we demonstrate that Smc5-Smc6 is necessary to suppress gross chromosomal rearrangements. Our findings show that the Smc5-Smc6 complex is essential for genome stability as it promotes repair of DSBs by error-free sister-chromatid recombination (SCR), thereby suppressing inappropriate non-sister recombination events.
De Piccoli
Giacomo
G
Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK.
Cortes-Ledesma
Felipe
F
Ira
Gregory
G
Torres-Rosell
Jordi
J
Uhle
Stefan
S
Farmer
Sarah
S
Hwang
Ji-Young
JY
Machin
Felix
F
Ceschia
Audrey
A
McAleenan
Alexandra
A
Cordon-Preciado
Violeta
V
Clemente-Blanco
Andrés
A
Vilella-Mitjana
Felip
F
Ullal
Pranav
P
Jarmuz
Adam
A
Leitao
Beatriz
B
Bressan
Debra
D
Dotiwala
Farokh
F
Papusha
Alma
A
Zhao
Xiaolan
X
Myung
Kyungjae
K
Haber
James E
JE
Aguilera
Andrés
A
Aragón
Luis
L
D018797:Cell Cycle Proteins / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D042822:Genomic Instability; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D012854:Sister Chromatid Exchange
D018797:Cell Cycle Proteins; C486627:SMC5 protein, S cerevisiae; C486631:SMC6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific