title: MR tracking of transplanted glial cells using poly-L-lysine-CF3.
abstract: Magnetic resonance (MR) imaging using super-paramagnetic iron oxides (SPIOs) is a powerful tool to monitor transplanted cells in living animals. Since, however, SPIOs are negative contrast agents, positive agents have been explored. In this study, we examined the feasibility of FITC-labeled poly-L-lysine-CF3 (PLK-CF3) using glial cells. FITC-labeled PLK-CF3 was easily internalized by neuroblastoma cells and glia as adding it into culture medium. No toxicity was seen at the concentration of less than 80 microg/ml. MR images positively detected labeled cells transplanted in the brain of living mouse. The results indicate that FITC-labeled PLK-CF3 is a useful positive contrast agent for MR tracking.
authors.affiliation: Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu 520-2192, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Masuda
authors.forename: Zyunichi
authors.initials: Z
authors.lastname: Maki
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Morikawa
authors.forename: Masahito
authors.initials: M
authors.lastname: Morita
authors.forename: Toshiro
authors.initials: T
authors.lastname: Inubushi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Matsusue
authors.forename: Sonoko
authors.initials: S
authors.lastname: Yamagata
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Taguchi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Doi
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shirai
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirao
authors.forename: Ikuo
authors.initials: I
authors.lastname: Tooyama
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D003287:Contrast Media; D052203:Ferrosoferric Oxide / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009457:Neuroglia / Q000502:physiology / Q000637:transplantation*; D011107:Polylysine / Q000378:metabolism*; D051381:Rats
chemical_list: D003287:Contrast Media; D011107:Polylysine; D052203:Ferrosoferric Oxide