title: Early legume responses to inoculation with Rhizobium sp. NGR234.
abstract: Interactions between legumes and rhizobia are controlled by the sequential exchange of symbiotic signals. Two different techniques, 2D-PAGE electrophoresis and differential display were used to study the effects of rhizobial signals on legume development. Application of variously substituted lipo-oligo-saccharidic Nod-factors to roots of Vigna unguiculata resulted in changes in the phosphorylation patterns of microsomal proteins. Reliable amino-acid sequences were obtained for one Nod-factor enhanced protein which was highly homologous to the 57-kDa subunit from Arabidopsis thaliana vacuolar membrane H(+)-ATPase. Immuno-blotting techniques demonstrated that Nod-factors cause rapid and massive increases of this enzyme in treated roots, suggesting that H(+)-ATPases play symbiotic roles. Concomitantly, we used differential display (DD) techniques on mRNA isolated from root-hairs to analyse early root responses to NGR234. Significant matches of several DD clones to known sequences were found. Clone D2.62 was homologous to a multitude of receptor kinases including S receptor-like kinases of A. thaliana and clone D4.1 showed similarities to Lotus japonicus phosphatidylinositol transfer-like protein III and late nodulin 16. Independent confirmatory analyses of these differentially expressed clones indicated expression at very low levels.
authors.affiliation: LBMPS, Université de Genève, Sciences III, 30 quai Ernest-Ansermet, 1211 Genève 4, Switzerland.
authors.forename: N M
authors.initials: NM
authors.lastname: Boukli
authors.forename: E
authors.initials: E
authors.lastname: Sunderasan
authors.forename: A
authors.initials: A
authors.lastname: Bartsev
authors.forename: D
authors.initials: D
authors.lastname: Hochstrasser
authors.forename: X
authors.initials: X
authors.lastname: Perret
authors.forename: A J
authors.initials: AJ
authors.lastname: Bjourson
authors.forename: A
authors.initials: A
authors.lastname: Krause
authors.forename: W J
authors.initials: WJ
authors.lastname: Broughton
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D015180:Electrophoresis, Gel, Two-Dimensional; D007887:Fabaceae / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D020869:Gene Expression Profiling; D015151:Immunoblotting; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D012231:Rhizobium / Q000502:physiology*; D020539:Sequence Analysis, Protein; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D013559:Symbiosis
chemical_list: D012333:RNA, Messenger