Synthesis and application of quantum dots FRET-based protease sensors.
Preparation of FRET-based quantum dots as protease sensors-RGDC peptide molecules are bound to the surface of CdSe/ZnS quantum dots. The peptide molecules are then labeled with rhodamine dye molecules. The emission color of the quantum dots change from green to orange due to fluorescence resonance energy transfer (FRET) between the quantum dots and the bound rhodamine molecules. Cleavage of the peptide by selective proteases releases the rhodamine molecules from the quantum dots surface, which results in decreasing FRET efficiency between the quantum dots and the rhodamine molecules. The emission color of the quantum dots changes back to green.
Shi
Lifang
L
Department of Chemistry and the Advanced Material Research Institute (AMRI), University of New Orleans, New Orleans, Louisiana 70148, USA.
De Paoli
Vania
V
Rosenzweig
Nitsa
N
Rosenzweig
Zeev
Z
D004789:Enzyme Activation; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D008666:Metalloendopeptidases / Q000032:analysis / Q000737:chemistry; D010447:Peptide Hydrolases / Q000032:analysis / Q000737:chemistry*; D045663:Quantum Dots; D012235:Rhodamines / Q000737:chemistry
D005456:Fluorescent Dyes; D012235:Rhodamines; C103220:rhodamine red-X succinimidyl ester; D010447:Peptide Hydrolases; D008666:Metalloendopeptidases