title: [Construction of the recombinant adenovirus vector with EGFR antisense cDNA of controlling cell cycle].
abstract: OBJECTIVE
  To recombine the adenovirus vector carrying EGFR sence/antisense cDNA which takes part in control of cell cycle.
  
  
  METHODS
  The 1032 bp EGFR sence/antisense cDNA fragment was cloned into the shuttle plasmid pAdTrack-CMV. The resultant plasmid and the backbone plasmid pAdEasy-1 were transferred into E. coli BJ5183 for homologous recombination, and the recombinant adenoviruses were generated in cells. The recombinant adenoviruses were packaged and amplified in the 293 cells. Then the viral titer was detected by GFP.
  
  
  RESULTS
  The recombinant adenovirus vector carrying EGFR sence/antisense cDNA to control the cell cycle was constructed successfully. The viral titers were 2.2 x 10(9) efu/mL and 2.5 X 10(9) efu/mL respectively.
  
  
  CONCLUSIONS
  The recombinant adenovirus vector constructed by us could introduce EGFR antisense cDNA into the laryngeal squamous cell carcinoma line or tumor tissue, which would provide an experiment basis to study further the interfered mechanism of signal transdution and the therapies of laryngeal squamous cell carcinoma.
authors.affiliation: Department of Otolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jun-Ming
authors.initials: JM
authors.lastname: Xian
authors.forename: Guang-yao
authors.initials: GY
authors.lastname: Zhou
authors.forename: Chuan-yu
authors.initials: CY
authors.lastname: Liang
authors.forename: Shi-xi
authors.initials: SX
authors.lastname: Liu
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002453:Cell Cycle; D018076:DNA, Complementary / Q000235:genetics; D004622:Embryo, Mammalian; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D007668:Kidney / Q000166:cytology; D007822:Laryngeal Neoplasms / Q000473:pathology; D016376:Oligonucleotides, Antisense / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D011994:Recombinant Proteins; D066246:ErbB Receptors