Initial characterization of a bolA homologue from Pseudomonas fluorescens indicates different roles for BolA-like proteins in P. fluorescens and Escherichia coli.
The RpoS-regulated bolA gene in Escherichia coli is important for the decrease in cell size during stationary phase or sudden carbon starvation. A Pseudomonas fluorescens strain mutated in a gene with homology to bolA reduced its cell size upon carbon starvation, and RpoS had little effect on bolA expression. The mutant grew slower than the wild-type strain in minimal medium with L-serine as the sole nitrogen source, while growth rates were similar on a mixture of L-serine and L-cysteine. Reverse transcriptase polymerase chain reaction analysis indicated that the bolA homologue is the second gene in an operon where the two next ORFs encode putative proteins with homology to sulphurtransferases and protein disulphide isomerases. Complementation of the mutant phenotypes was only obtained by plasmids encoding BolA as well as the above two proteins. Growth phenotypes and gene homologies suggest that BolA-like proteins have different functions in E. coli and Pseudomonas.
Koch
Birgit
B
Genetics and Microbiology Group, Department of Ecology, The Royal Veterinary and Agricultural University, Frederiksberg C, Copenhagen, Denmark.
Nybroe
Ole
O
D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009876:Operon; D019704:Protein Disulfide-Isomerases / Q000235:genetics; D011551:Pseudomonas fluorescens / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D012329:RNA, Bacterial / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000502:physiology; D013466:Sulfurtransferases / Q000235:genetics; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D013466:Sulfurtransferases; D001616:beta-Galactosidase; D019704:Protein Disulfide-Isomerases