Role of glyceryl trinitrate, a nitric oxide donor, in the renal ischemia-reperfusion injury of rats.
OBJECTIVE
Ischemia-reperfusion injury is a serious clinical situation which can cause serious morbidity and mortality. An experimental renal ischemia-reperfusion injury model was designed to evaluate the role of glyceryl trinitrate (GTN) on renal function and histology.


METHODS
50 Wistar albino rats were used in our study. Five groups were formed: (1) sham-control group; (2) acute renal ischemia (ARI) group with placebo (0.9% NaCl) infusion; (3) GTN infusion with a 75 microg/kg/min dose prior to ARI was administered; (4) GTN infusion with a 150 microg/kg/min dose prior to ARI was given, and finally (5) 150 microg/kg/min GTN infusion after the ARI period was applied. Serum BUN and creatinine levels were measured for evaluation of renal function. T(max-sec), glomerular filtration rate (GFR), and T(max-min) results following a (99m)Tc-DTPA diuretic renal scintigraphy were used. Histological examination was performed on nephrectomy specimens.


RESULTS
Groups 2 and 5 showed higher BUN, creatinine, and lower GFR values than the other groups (p = 0.0001). There was no difference in BUN, creatinine, and GFR levels between groups 2 and 5 (p = 0.971, p = 0.739, p = 0.393). Also the T(max-sec) values were higher in groups 2 and 5 compared with the other groups (p = 0.0001). The presence of tubular necrosis was different between groups and was higher in groups 2 and 5 (p = 0.002).


CONCLUSIONS
The application of GTN, a nitric oxide donor, has caused significant improvement in renal function when applied prior to an experimentally designed renal ischemia-reperfusion model. But administration of GTN had no effect after occurrence of ischemia.
Kucuk
Hasan Fehmi
HF
Department of General Surgery, Kartal Research and Education Hospital, Istanbul, Turkey. hasan.kucuk@sbkeah.gov.tr
Kaptanoglu
Levent
L
Ozalp
Figen
F
Kurt
Necmi
N
Bingul
Sadik
S
Torlak
Oguzhan Aziz
OA
Colak
Elif
E
Akyol
Huseyin
H
Gul
Aylin Ege
AE
D000818:Animals; D001806:Blood Urea Nitrogen; D003401:Creatine / Q000097:blood; D005919:Glomerular Filtration Rate; D007668:Kidney / Q000473:pathology / Q000503:physiopathology*; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors; D005996:Nitroglycerin; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology*
D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D005996:Nitroglycerin; D003401:Creatine