Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses.
To enhance fungal disease resistance, wheat plants (cv. Bobwhite) were engineered to constitutively express the potent antimicrobial protein Ace-AMP1 from Allium cepa, driven by a maize ubiquitin promoter along with its first intron. The bar gene was used for selection of putative transformants on medium containing phosphinothricin (PPT). Transgene inheritance, integration and stability of expression were confirmed over two generations by PCR, Southern, northern and western blot analyses, respectively. The levels of Ace-AMP1 in different transgenic lines correlated with the transcript levels of the transgene. Up to 50% increase in resistance to Blumeria graminis f. sp. tritici was detected in detached leaf assays. In ears of transgenic wheat inoculated with Neovossia indica, Ace-AMP1 intensified expression of defense-related genes. Elevated levels of salicylic acid and of transcripts of phenylalanine ammonia lyase (PAL), glucanase (PR2) and chitinase (PR3) in the transgenic plants indicated manifestation of systemic acquired resistance (SAR).
Roy-Barman
Subhankar
S
Department of Microbiology and Biotechnology Centre, Faculty of Science, The M. S. University of Baroda, Vadodara 390 002, India.
Sautter
Christof
C
Chattoo
Bharat B
BB
D000935:Antifungal Agents / Q000494:pharmacology*; D015152:Blotting, Northern; D005821:Genetic Techniques; D005822:Genetic Vectors; D007113:Immunity, Innate; D008957:Models, Genetic; D010637:Phenolsulfonphthalein / Q000031:analogs & derivatives / Q000494:pharmacology; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020156:Salicylic Acid / Q000378:metabolism; D019076:Transgenes; D014908:Triticum / Q000235:genetics*
C096657:AMP1 protein, Allium cepa; D000935:Antifungal Agents; D010940:Plant Proteins; C007016:chlorophenol red; D010650:Phenylalanine Ammonia-Lyase; D010637:Phenolsulfonphthalein; D020156:Salicylic Acid