Designing, testing, and validating a microarray for stem cell characterization.
Microarray technology is a powerful tool that allows for simultaneous assessment of the expression of thousands of genes and identification of gene expression patterns associated with specific cell types. Here we describe a protocol using this method to examine stem cells.
Luo
Yongquan
Y
Stem Cell Biology Unit, Laboratory of Neurosciences, National Institute on Aging, Baltimore, MD, USA.
Bhattacharya
Bhaskar
B
Yang
Amy X
AX
Puri
Raj K
RK
Rao
Mahendra S
MS
D000818:Animals; D002454:Cell Differentiation; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards*; D015894:Genome, Human; D023281:Genomics / Q000379:methods / Q000592:standards; D006801:Humans; D006824:Hybridization, Genetic; D051379:Mice; D009474:Neurons / Q000166:cytology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D011786:Quality Control