Development and evaluation of a diagnostic PCR for Mycoplasma synoviae using primers located in the intergenic spacer region and the 23S rRNA gene.
Mycoplasma synoviae (Ms) is an important pathogen of poultry, causing economic losses to this industry. Early and reliable diagnosis is a key to controlling the spread of this organism. In this study, a polymerase chain reaction with one primer based on the intergenic spacer region (ISR) was validated for detection of Ms. The ISR primer was paired with a general primer from within the 23S rRNA gene. The PCR primers were tested with the 22 other recognised avian Mycoplasma species to check the specificity and with 21 field isolates of Ms from various hosts and countries, and with several swab samples. The PCR appeared to be specific and sensitive. Four different sample preparation methods were compared for use in this PCR, and the amplification protocol was compared with three others, confirming the comparative sensitivity of the new PCR.
Ramírez
Ana S
AS
Department of Veterinary Pathology, University of Liverpool, Jordan Bldg, Neston CH64 7TE, UK.
Naylor
Clive J
CJ
Hammond
Philip P
PP
Bradbury
Janet M
JM
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D017931:DNA Primers; D021901:DNA, Intergenic; D005784:Gene Amplification; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D045805:Mycoplasma synoviae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014422:Turkeys
D017931:DNA Primers; D021901:DNA, Intergenic; D012338:RNA, Ribosomal, 23S