SNP genotyping by multiplex amplification and microarrays assay for forensic application.
OBJECTIVE
Research on the application feasibility of SNP genotyping for forensic identification by microarrays.


METHODS
Oligonucleotide microarrays which could detect 34 different SNPs were used. After hybridization and washing, the arrays were scanned and fluorescence intensities analyzed using Microarray software. Population studies on 34 SNP loci were carried out in a sample of 109 unrelated Chinese Han individuals using oligonucleotide microarrays for genotype detection. The method was also applied to cases.


RESULTS
According to the results of population studies, no deviations from Hardy-Weinberg equilibrium could be found. Among the 34 loci, 3 SNPs were low informative, 4 were medium informative and 27 were high informative. The combination discrimination power (CDP) of the 31 optimal polymorphic SNPs was 0.9999999999979. The matching probability was 2.13 x 10(-12). The average exclusion probability in paternity testing for duos was 0.9609. The average exclusion probability in paternity testing for trios was 0.9970.


CONCLUSIONS
The data and case application demonstrated that SNP typing by oligonucleotide probe microarrays was a useful technique for paternity testing and individual identification. Combined with the 28 SNPs loci distributed on HLA-DRB1 and ABO genes, the combination discrimination power (CDP) was 0.9999999999999910. The matching probability was 9.02 x 10(-15). The average exclusion probabilities in duos and in trios were 0.9894 and 0.9992, respectively. It may be concluded that the 59 SNPs loci yield the same power in forensic identification as CODIS STRs currently used.
Li
Li
L
Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China.
Li
Cheng-Tao
CT
Li
Rong-Yu
RY
Liu
Yan
Y
Lin
Yuan
Y
Que
Ting-Zhi
TZ
Sun
Mei-Qian
MQ
Li
Yao
Y
D002681:China; D016172:DNA Fingerprinting; D005006:Ethnicity / Q000235:genetics; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D012984:Software