Nitrile hydrolysis activity of Rhodococcus erythropolis NCIMB 11540 whole cells.
The nitrile hydrolyzing properties of the bacterium strain Rhodococcus erythropolis NCIMB 11540 have been investigated. Using whole cells of the microorganism, a wide variety of aromatic and aliphatic cyanide-containing substrates was successfully hydrolyzed to the corresponding amide or acid. In the case of dicyanides, selective monohydrolysis took place, which was further explored in the desymmetrization of malononitriles resulting in the corresponding cyano amides in enantiomeric excesses of up to 98%.
Vink
Mandy K S
MK
J.H. van 't Hoff Institute for Molecular Sciences, University of Amsterdam, Amsterdam, The Netherlands.
Wijtmans
Roel
R
Reisinger
Christoph
C
van den Berg
Rutger J F
RJ
Schortinghuis
Christien A
CA
Schwab
Helmut
H
Schoemaker
Hans E
HE
Rutjes
Floris P J T
FP
D006868:Hydrolysis; D009570:Nitriles / Q000378:metabolism*; D012240:Rhodococcus / Q000145:classification* / Q000378:metabolism*; D013045:Species Specificity
D009570:Nitriles