Primitive and bone metastatic renal carcinoma cells promote osteoclastogenesis through endothelial cells.
BACKGROUND
The contribution of angiogenesis to renal carcinoma bone metastases is virtually unknown.


METHODS
The effect of a cell line from a renal carcinoma bone metastasis (CRBM) was compared in vitro with the primitive renal adenocarcinoma line ACHN, by evaluating the influence on the ability of bone endothelial cells to activate osteoclasts.


RESULTS
The ACHN-conditioned medium produced a significant expression of macrophage-colony-stimulating factor mRNA. The conditioned medium from ACHN, CRBM, or from endothelial cells previously stimulated with the neoplastic cell-conditioned media, had no direct effect on osteoclast differentiation from blood precursors (PBMC), such as CRBM and ACHN co-cultured with PBMC. However, PBMC co-cultured with endothelial cells previously stimulated with the CRBM-conditioned medium showed significantly higher levels of tartrate-resistant acid phosphatase.


CONCLUSIONS
It is possible that the bone metastatic line CRBM releases factors that induce endothelial cells to favor osteoclast differentiation.
Cenni
Elisabetta
E
Laboratorio di Fisiopatologia degli Impianti Ortopedici, Istituti Ortopedici Rizzoli, Bologna, Italy. elisabetta.cenni@ior.it
Perut
Francesca
F
Granchi
Donatella
D
Amato
Ilaria
I
Avnet
Sofia
S
Brandi
Maria Luisa
ML
Giunti
Armando
A
Baldini
Nicola
N
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001859:Bone Neoplasms / Q000097:blood / Q000098:blood supply / Q000201:enzymology / Q000556:secondary*; D002292:Carcinoma, Renal Cell / Q000097:blood / Q000098:blood supply / Q000201:enzymology / Q000473:pathology* / Q000556:secondary; D002417:Cattle; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned; D042783:Endothelial Cells / Q000201:enzymology / Q000473:pathology*; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D007527:Isoenzymes / Q000378:metabolism; D007680:Kidney Neoplasms / Q000097:blood / Q000098:blood supply / Q000201:enzymology / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology; D010010:Osteoclasts / Q000201:enzymology / Q000473:pathology; D000071681:Tartrate-Resistant Acid Phosphatase
D017077:Culture Media, Conditioned; D015850:Interleukin-6; D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase