Dynamic HPLC on chiral stationary phases: a powerful tool for the investigation of stereomutation processes.
Dynamic HPLC on enantioselective stationary phases has become a well-established technique to investigate chiral molecules with internal motions that result in stereoinversion and occur on the time scale of the separation process. Kinetic parameters for the on-column interconversion phenomena can be extracted from experimental peak profiles by computer simulation or by direct calculation methods. The technique has been used in a wide range of temperatures and is complementary in scope to dynamic NMR spectroscopy.
D'Acquarica
Ilaria
I
Dipartimento di Studi di Chimica e Tecnologia delle Sostanze Biologicamente Attive, Università degli Studi di Roma La Sapienza, Rome, Italy.
Gasparrini
Francesco
F
Pierini
Marco
M
Villani
Claudio
C
Zappia
Giovanni
G
D000577:Amides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D008962:Models, Theoretical; D015394:Molecular Structure; D010087:Oxides / Q000737:chemistry; D013237:Stereoisomerism; D013696:Temperature
D000577:Amides; D010087:Oxides