In vitro antibacterial activity of Lactobacillus helveticus strain KS300 against diarrhoeagenic, uropathogenic and vaginosis-associated bacteria.
OBJECTIVE
The purpose of this study was to investigate in vitro the antibacterial activity of the Lactobacillus helveticus strain KS300 against vaginosis-associated bacteria including Gardnerella vaginalis and Prevotella bivia, uropathogenic Escherichia coli, and diarrhoeagenic Salmonella enterica serovar Typhimurium.


RESULTS
The KS300 strain inhibited the growth of G. vaginalis, P. bivia, S. typhimurium, and pathogenic E. coli. After direct co-culture, data show that the Lactobacillus strain decreased the viability of G. vaginalis, P. bivia, S. typhimurium, and pathogenic E. coli. The adhering KS300 strain inhibited the adhesion of G. vaginalis DSM 4944 and uropathogenic Dr-positive E. coli IH11128 onto HeLa cells. Moreover, the KS300 strain inhibited the internalization of uropathogenic Dr-positive E. coli IH11128 within HeLa cells and S. typhimurium SL1344 within Caco-2/TC7 cells.


CONCLUSIONS
The findings demonstrate that L. helveticus strain KS300 is adhesive onto cultured human cells and has antagonistic activities against vaginosis-associated, uropathogenic and diarrhoeagenic pathogens.


CONCLUSIONS
Adhering L. helveticus strain KS300 is a potential probiotic strain displaying a strain-specific array of in vitro antibacterial activities.
Atassi
F
F
Institut National de la Santé et de la Recherche Médicale, Unité 756, Faculté de Pharmacie, Université Paris, Châtenay-Malabry, France.
Brassart
D
D
Grob
P
P
Graf
F
F
Servin
A L
AL
D001422:Bacterial Adhesion / Q000502:physiology; D018938:Caco-2 Cells; D018920:Coculture Techniques / Q000379:methods; D003967:Diarrhea / Q000178:diet therapy / Q000382:microbiology*; D004926:Escherichia coli / Q000254:growth & development; D005260:Female; D006194:Gardnerella vaginalis / Q000254:growth & development; D006367:HeLa Cells; D006801:Humans; D052198:Lactobacillus helveticus / Q000502:physiology*; D018720:Prevotella / Q000254:growth & development; D019936:Probiotics / Q000627:therapeutic use*; D012486:Salmonella typhimurium / Q000254:growth & development; D014570:Urologic Diseases / Q000178:diet therapy / Q000382:microbiology*; D016585:Vaginosis, Bacterial / Q000178:diet therapy / Q000382:microbiology*